LOCUS       DACCCS010000001       113899 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.001, whole genome shotgun sequence.
ACCESSION   DACCCS010000001 DACCCS010000000
VERSION     DACCCS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 113899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 113899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..113899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.001"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            173..445
                     /locus_tag="IVK82_000001"
     CDS             173..445
                     /locus_tag="IVK82_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391101.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            460..1383
                     /locus_tag="IVK82_000002"
     CDS             460..1383
                     /locus_tag="IVK82_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6391102.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(1523..2275)
                     /locus_tag="IVK82_000003"
     CDS             complement(1523..2275)
                     /locus_tag="IVK82_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6391103.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            2498..2860
                     /locus_tag="IVK82_000004"
     CDS             2498..2860
                     /locus_tag="IVK82_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP6391104.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            3497..4969
                     /locus_tag="IVK82_000005"
     CDS             3497..4969
                     /locus_tag="IVK82_000005"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP6391105.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            4981..5805
                     /gene="nadE"
                     /locus_tag="IVK82_000006"
     CDS             4981..5805
                     /gene="nadE"
                     /locus_tag="IVK82_000006"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP6391106.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(5878..6768)
                     /locus_tag="IVK82_000007"
     CDS             complement(5878..6768)
                     /locus_tag="IVK82_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP6391107.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            6966..8483
                     /locus_tag="IVK82_000008"
     CDS             6966..8483
                     /locus_tag="IVK82_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP6391108.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            8473..9744
                     /locus_tag="IVK82_000009"
     CDS             8473..9744
                     /locus_tag="IVK82_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6391109.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            9919..10815
                     /locus_tag="IVK82_000010"
     CDS             9919..10815
                     /locus_tag="IVK82_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6391110.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            10829..11692
                     /locus_tag="IVK82_000011"
     CDS             10829..11692
                     /locus_tag="IVK82_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6391111.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            11698..12261
                     /locus_tag="IVK82_000012"
     CDS             11698..12261
                     /locus_tag="IVK82_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP6391112.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            12271..13794
                     /locus_tag="IVK82_000013"
     CDS             12271..13794
                     /locus_tag="IVK82_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP6391113.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            13806..14765
                     /locus_tag="IVK82_000014"
     CDS             13806..14765
                     /locus_tag="IVK82_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP6391114.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            14851..15930
                     /locus_tag="IVK82_000015"
     CDS             14851..15930
                     /locus_tag="IVK82_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6391115.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(16182..17582)
                     /locus_tag="IVK82_000016"
     CDS             complement(16182..17582)
                     /locus_tag="IVK82_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP6391116.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            17800..19461
                     /locus_tag="IVK82_000017"
     CDS             17800..19461
                     /locus_tag="IVK82_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP6391117.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            19418..20116
                     /locus_tag="IVK82_000018"
     CDS             19418..20116
                     /locus_tag="IVK82_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP6391118.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            20132..21556
                     /gene="araA"
                     /locus_tag="IVK82_000019"
     CDS             20132..21556
                     /gene="araA"
                     /locus_tag="IVK82_000019"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP6391119.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            21863..23022
                     /locus_tag="IVK82_000020"
                     /pseudo
     CDS             21863..23022
                     /locus_tag="IVK82_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            23140..24999
                     /locus_tag="IVK82_000021"
     CDS             23140..24999
                     /locus_tag="IVK82_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP6391120.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            25109..25543
                     /locus_tag="IVK82_000022"
     CDS             25109..25543
                     /locus_tag="IVK82_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391121.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(25982..27004)
                     /locus_tag="IVK82_000023"
     CDS             complement(25982..27004)
                     /locus_tag="IVK82_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP6391122.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            27132..27485
                     /locus_tag="IVK82_000024"
     CDS             27132..27485
                     /locus_tag="IVK82_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP6391123.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            27497..28663
                     /locus_tag="IVK82_000025"
     CDS             27497..28663
                     /locus_tag="IVK82_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6391124.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(28755..29375)
                     /locus_tag="IVK82_000026"
     CDS             complement(28755..29375)
                     /locus_tag="IVK82_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP6391125.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(29547..29843)
                     /locus_tag="IVK82_000027"
     CDS             complement(29547..29843)
                     /locus_tag="IVK82_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP6391126.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            30047..30697
                     /locus_tag="IVK82_000028"
     CDS             30047..30697
                     /locus_tag="IVK82_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6391127.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            30713..32440
                     /locus_tag="IVK82_000029"
     CDS             30713..32440
                     /locus_tag="IVK82_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6391128.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            32437..34329
                     /locus_tag="IVK82_000030"
     CDS             32437..34329
                     /locus_tag="IVK82_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6391129.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            34788..35657
                     /locus_tag="IVK82_000031"
     CDS             34788..35657
                     /locus_tag="IVK82_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP6391130.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            35866..36891
                     /locus_tag="IVK82_000032"
     CDS             35866..36891
                     /locus_tag="IVK82_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP6391131.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            36930..37880
                     /gene="manA"
                     /locus_tag="IVK82_000033"
     CDS             36930..37880
                     /gene="manA"
                     /locus_tag="IVK82_000033"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP6391132.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            37974..39677
                     /locus_tag="IVK82_000034"
     CDS             37974..39677
                     /locus_tag="IVK82_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6391133.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            39679..41712
                     /locus_tag="IVK82_000035"
     CDS             39679..41712
                     /locus_tag="IVK82_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6391134.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            41723..42163
                     /locus_tag="IVK82_000036"
     CDS             41723..42163
                     /locus_tag="IVK82_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6391135.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(42363..43490)
                     /locus_tag="IVK82_000037"
     CDS             complement(42363..43490)
                     /locus_tag="IVK82_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6391136.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(43493..44170)
                     /locus_tag="IVK82_000038"
     CDS             complement(43493..44170)
                     /locus_tag="IVK82_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6391137.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            44434..46200
                     /locus_tag="IVK82_000039"
     CDS             44434..46200
                     /locus_tag="IVK82_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP6391138.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            46215..46652
                     /locus_tag="IVK82_000040"
     CDS             46215..46652
                     /locus_tag="IVK82_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6391139.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            46665..47816
                     /locus_tag="IVK82_000041"
     CDS             46665..47816
                     /locus_tag="IVK82_000041"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP6391140.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(47878..48576)
                     /locus_tag="IVK82_000042"
     CDS             complement(47878..48576)
                     /locus_tag="IVK82_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP6391141.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(48569..49921)
                     /locus_tag="IVK82_000043"
     CDS             complement(48569..49921)
                     /locus_tag="IVK82_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP6391142.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(50144..51544)
                     /locus_tag="IVK82_000044"
     CDS             complement(50144..51544)
                     /locus_tag="IVK82_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP6391143.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            51705..52442
                     /locus_tag="IVK82_000045"
     CDS             51705..52442
                     /locus_tag="IVK82_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP6391144.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            52567..53046
                     /locus_tag="IVK82_000046"
     CDS             52567..53046
                     /locus_tag="IVK82_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP6391145.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            53043..53810
                     /locus_tag="IVK82_000047"
     CDS             53043..53810
                     /locus_tag="IVK82_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391146.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            54040..54609
                     /gene="lepB"
                     /locus_tag="IVK82_000048"
     CDS             54040..54609
                     /gene="lepB"
                     /locus_tag="IVK82_000048"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6391147.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(54713..55315)
                     /locus_tag="IVK82_000049"
     CDS             complement(54713..55315)
                     /locus_tag="IVK82_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP6391148.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            55421..55890
                     /locus_tag="IVK82_000050"
                     /pseudo
     CDS             55421..55890
                     /locus_tag="IVK82_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            55996..56580
                     /locus_tag="IVK82_000051"
     CDS             55996..56580
                     /locus_tag="IVK82_000051"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP6391149.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            56583..57656
                     /gene="prfA"
                     /locus_tag="IVK82_000052"
     CDS             56583..57656
                     /gene="prfA"
                     /locus_tag="IVK82_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP6391150.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            57649..58488
                     /gene="prmC"
                     /locus_tag="IVK82_000053"
     CDS             57649..58488
                     /gene="prmC"
                     /locus_tag="IVK82_000053"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP6391151.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            58481..58660
                     /locus_tag="IVK82_000054"
     CDS             58481..58660
                     /locus_tag="IVK82_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391152.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            58682..59707
                     /locus_tag="IVK82_000055"
     CDS             58682..59707
                     /locus_tag="IVK82_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP6391153.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            59882..61126
                     /locus_tag="IVK82_000056"
     CDS             59882..61126
                     /locus_tag="IVK82_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP6391154.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            61279..62226
                     /locus_tag="IVK82_000057"
     CDS             61279..62226
                     /locus_tag="IVK82_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6391155.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            62360..62989
                     /gene="upp"
                     /locus_tag="IVK82_000058"
     CDS             62360..62989
                     /gene="upp"
                     /locus_tag="IVK82_000058"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP6391156.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(63128..63370)
                     /locus_tag="IVK82_000059"
     CDS             complement(63128..63370)
                     /locus_tag="IVK82_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391157.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            63491..63563
                     /locus_tag="IVK82_000060"
     tRNA            63491..63563
                     /locus_tag="IVK82_000060"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63524..63526,aa:Thr,seq:cgt)
     gene            complement(63682..64320)
                     /locus_tag="IVK82_000061"
     CDS             complement(63682..64320)
                     /locus_tag="IVK82_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP6391158.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(64443..65075)
                     /locus_tag="IVK82_000062"
     CDS             complement(64443..65075)
                     /locus_tag="IVK82_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP6391159.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            65426..65725
                     /locus_tag="IVK82_000063"
     CDS             65426..65725
                     /locus_tag="IVK82_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP6391160.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            66220..68385
                     /gene="acm"
                     /locus_tag="IVK82_000064"
     CDS             66220..68385
                     /gene="acm"
                     /locus_tag="IVK82_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP6391161.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(68657..69142)
                     /locus_tag="IVK82_000065"
     CDS             complement(68657..69142)
                     /locus_tag="IVK82_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP6391162.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            69622..70263
                     /locus_tag="IVK82_000066"
     CDS             69622..70263
                     /locus_tag="IVK82_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6391163.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            70282..70917
                     /locus_tag="IVK82_000067"
     CDS             70282..70917
                     /locus_tag="IVK82_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6391164.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            70935..71750
                     /locus_tag="IVK82_000068"
     CDS             70935..71750
                     /locus_tag="IVK82_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6391165.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            72069..73505
                     /locus_tag="IVK82_000069"
     CDS             72069..73505
                     /locus_tag="IVK82_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6391166.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            73694..76141
                     /locus_tag="IVK82_000070"
     CDS             73694..76141
                     /locus_tag="IVK82_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP6391167.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            76371..77438
                     /gene="rlmN"
                     /locus_tag="IVK82_000071"
     CDS             76371..77438
                     /gene="rlmN"
                     /locus_tag="IVK82_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP6391168.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(77631..78734)
                     /locus_tag="IVK82_000072"
     CDS             complement(77631..78734)
                     /locus_tag="IVK82_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6391169.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            79287..79835
                     /gene="aac(6')"
                     /locus_tag="IVK82_000073"
     CDS             79287..79835
                     /gene="aac(6')"
                     /locus_tag="IVK82_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP6391170.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            80381..82042
                     /locus_tag="IVK82_000074"
     CDS             80381..82042
                     /locus_tag="IVK82_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6391171.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(82112..82459)
                     /locus_tag="IVK82_000075"
     CDS             complement(82112..82459)
                     /locus_tag="IVK82_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP6391172.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            82543..83484
                     /locus_tag="IVK82_000076"
     CDS             82543..83484
                     /locus_tag="IVK82_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6391173.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            83485..84528
                     /locus_tag="IVK82_000077"
     CDS             83485..84528
                     /locus_tag="IVK82_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6391174.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            84534..85604
                     /locus_tag="IVK82_000078"
     CDS             84534..85604
                     /locus_tag="IVK82_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6391175.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            85594..86547
                     /locus_tag="IVK82_000079"
     CDS             85594..86547
                     /locus_tag="IVK82_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6391176.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            86559..87569
                     /locus_tag="IVK82_000080"
     CDS             86559..87569
                     /locus_tag="IVK82_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6391177.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(87854..88675)
                     /locus_tag="IVK82_000081"
     CDS             complement(87854..88675)
                     /locus_tag="IVK82_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP6391178.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(88772..89050)
                     /locus_tag="IVK82_000082"
     CDS             complement(88772..89050)
                     /locus_tag="IVK82_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391179.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            89318..91327
                     /gene="metG"
                     /locus_tag="IVK82_000083"
     CDS             89318..91327
                     /gene="metG"
                     /locus_tag="IVK82_000083"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP6391180.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(91806..92393)
                     /locus_tag="IVK82_000084"
     CDS             complement(91806..92393)
                     /locus_tag="IVK82_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391181.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            92568..93890
                     /locus_tag="IVK82_000085"
     CDS             92568..93890
                     /locus_tag="IVK82_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6391182.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            94045..94821
                     /locus_tag="IVK82_000086"
     CDS             94045..94821
                     /locus_tag="IVK82_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP6391183.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            94814..95383
                     /gene="rnmV"
                     /locus_tag="IVK82_000087"
     CDS             94814..95383
                     /gene="rnmV"
                     /locus_tag="IVK82_000087"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP6391184.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            95376..96260
                     /gene="rsmA"
                     /locus_tag="IVK82_000088"
     CDS             95376..96260
                     /gene="rsmA"
                     /locus_tag="IVK82_000088"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP6391185.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            96711..97340
                     /locus_tag="IVK82_000089"
     CDS             96711..97340
                     /locus_tag="IVK82_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391186.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(97464..98573)
                     /gene="ugpC"
                     /locus_tag="IVK82_000090"
     CDS             complement(97464..98573)
                     /gene="ugpC"
                     /locus_tag="IVK82_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP6391187.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            98782..99204
                     /gene="mgsA"
                     /locus_tag="IVK82_000091"
     CDS             98782..99204
                     /gene="mgsA"
                     /locus_tag="IVK82_000091"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP6391188.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            99527..100063
                     /locus_tag="IVK82_000092"
     CDS             99527..100063
                     /locus_tag="IVK82_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP6391189.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(100103..101866)
                     /locus_tag="IVK82_000093"
     CDS             complement(100103..101866)
                     /locus_tag="IVK82_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6391190.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(101869..103584)
                     /locus_tag="IVK82_000094"
     CDS             complement(101869..103584)
                     /locus_tag="IVK82_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6391191.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(104363..104587)
                     /locus_tag="IVK82_000095"
     CDS             complement(104363..104587)
                     /locus_tag="IVK82_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391192.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            104787..107555
                     /locus_tag="IVK82_000096"
     CDS             104787..107555
                     /locus_tag="IVK82_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6391193.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            107636..108055
                     /locus_tag="IVK82_000097"
     CDS             107636..108055
                     /locus_tag="IVK82_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP6391194.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            108067..108537
                     /locus_tag="IVK82_000098"
     CDS             108067..108537
                     /locus_tag="IVK82_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6391195.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            108554..109333
                     /locus_tag="IVK82_000099"
     CDS             108554..109333
                     /locus_tag="IVK82_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6391196.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            109320..110141
                     /locus_tag="IVK82_000100"
     CDS             109320..110141
                     /locus_tag="IVK82_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6391197.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            110158..111207
                     /locus_tag="IVK82_000101"
     CDS             110158..111207
                     /locus_tag="IVK82_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6391198.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            111220..112224
                     /locus_tag="IVK82_000102"
     CDS             111220..112224
                     /locus_tag="IVK82_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6391199.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            112857..113876
                     /locus_tag="IVK82_000103"
     CDS             112857..113876
                     /locus_tag="IVK82_000103"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP6391200.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        37820 a        19739 c        23400 g        32940 t
ORIGIN      
        1 cattcactaa taataaagat atatttacag tatcattata atgtattcaa tttaaaataa
       61 gagactttgg taataatgaa agaggaataa aacttttctt tggcaaaaca aactccttgc
      121 tatgagacgc gtttcataca ctatactcaa agtatcaaaa ggagggcttg caatgaatac
      181 ttatgtagag ataacagatg gcacaacgaa caattatttt tatgcgtttg tcgagttcaa
      241 gggatccgta ttaacgttgt acagttttca agggttgaat aggaatatag taaaagaaat
      301 accaatgaat gaaatcgaaa atttgacaaa agatatatat tggggcgggc agcggatcag
      361 tttttcgcat gaaggaaaaa tgtatcagtt ttttgaatgc ggtccagctg tggtagacta
      421 tttacaagaa aatctttttg tataggtggc gaagcagcag tggcaaacat tcgagatatc
      481 gcaaagctca caggttattc agtttctact atttcacgtg taatcaatgg acatccatat
      541 gtagatgagg aaaaaaggaa agaaatactt gcggtcatga aagaagtcga ttatatccct
      601 aatgcaaatg ccagacagtt aagttatgga aaaacgaaaa gcattggagt gattctgcct
      661 tataccaatc atccgtattt tgatcagttg atcagtggga tcattgaagc agcgtttgat
      721 tatgattaca aagtgacact tttaccgacg ggctatcata aagaaaaaga gcgaaggtat
      781 cttgaagaat ttgcagctaa agctttcgat ggattgatca ttacgtctcg tgccaataca
      841 ctagatgatt tattggcata tcaaaaatat ggacctctag ttttttgtga agaaatcaag
      901 ggaaaacagg caagctgtgt ctttattgat cgagaacaat caattacaga aggtcttact
      961 tatttgaagc agcaaggtat aaagcaattg ggcatcacgt tggggcgaag tggcaggcta
     1021 agttataact caaaaatcac tttgcagcta tgtcatcaac tttttccatc ttttgatgaa
     1081 tcacttgttt tttgggattg tatagatgca gagaaaggaa aacaagcagg cttctttttt
     1141 aaagagcttg gagttgatgg tgtctttaca aattcggata tggttgcagc aggcatcttg
     1201 caaagctatc tgcaagataa gacaccagtg gtgattggac gcgataattt gctgatcagc
     1261 gagcttcttg gtttcccaac catcgaccac catctggaag cttgcggaga aatggcattt
     1321 caattgtttc ttacagaaaa aaaggataaa gtgaaaatac cttatacgtt tattcaacga
     1381 tgaatatatt taaagtagga tgaaaaaact tgtgaaagaa tggagcagac accaaaagtt
     1441 agcataaaaa tctaacttgg gtgtctgctg ttttgttatt aggtcatttt acgtgtactt
     1501 ttttgttgga tcatgtaaaa gattacgcct ttttttggat aatttcattt aaagcttttg
     1561 ctgcaccatg accacttcgc tcgtcataat atgcagtgaa ccgttcatcg gcttcgtaca
     1621 tcattcctag tcctttatgc gcttttggct gatattcagg ccagctgtaa gagaaccact
     1681 tcttatgagc ttggaagata cggtctgcta agctttcatc ttctggttct tttaagtaaa
     1741 gagtgagatc aaataacatc tgcttttcaa cagtttgcat ttcttcatac gtttctttat
     1801 ccatattcat ccatttttca ttggatgctg taaccttttt ttttccgtat ttttgtcgaa
     1861 tctcttgtcc ataggattct tcattttctt tcaacttttg ttgtttaaac ccttcaaatt
     1921 tttctttgtc actcattttt cttcctcctt cgtacatatc aagtgtctgt tggacagttg
     1981 tcaagagggt atcgatttgt gcccgttttt ccagcaacat ttgctgatgc ttgattaaag
     2041 ctttctggtg gtcaaaagat ggattgtcta agacctcttg gatatcatcc aacttgaatg
     2101 ccattgttcg ataaaataag atgtgttgca gacgatcgac ttcttttttg ccataaatcc
     2161 gataacctga agaattgatc ctagctggtt ttagcagtcc aatctcatca taaaagcgca
     2221 aggttctcgg actgactcct gatatctctg acattttctt tattgtgtac tccattattt
     2281 ttcctcctga caaatgtaag cataatcctt tacgtaacga gatggtcaag aaaaaattgt
     2341 atttttttct cacgaaatta tgttatacta ttaagtgata aaaggtaaat ccttttgtct
     2401 ttaaaggttc aattgtaacc gattccttaa tgctaggaaa gatggtaaca taacttaaga
     2461 ttggatctaa atcgactata tagaaaagag gaatgagatg aagctaatcg aaacacctgt
     2521 gaatagtaat ttaaatatta agacatttta tcctaaagta gtggaattct tttttgggaa
     2581 tacggctatt aattattaca aattattttc gttagatcga acacaactgc tattggtaga
     2641 cacatatgat aaaaagcaag tcgtgatgat caatacgaag aaaaaaatca caaggcaaga
     2701 aatcgactat gcgattcatc acgttttaaa aatgacacga gaagatgtca aagtccatgt
     2761 aggagtgaaa caggagctag aacgtgccgg aatacaattc aaacgtccaa ataaagatat
     2821 tgtagtgata gaacaaaaaa atacgaagga ttctatttaa taacgactaa taatcatctt
     2881 tatggtaacc tgctccatat ctaatgtata caataaaaaa caaggcgaga ctgattgatg
     2941 tctcgccatt tttgtttcca ttctattgac agctgctttc ttactttgta tctttttaaa
     3001 aacgtgtgat attttttccg cgagtttttt caacgcaagg tatatcttgt cataattcgc
     3061 ctttccaaaa aaattcaata cataataagt tatatccgtt tctttgggga taagaagcaa
     3121 aaaaggaaag acgtattatt gcttcaaaaa cttttgccat tgattatgtt gtagagacat
     3181 ttggttatat ttgttcaatc atcggtatct taacggctac gattgtagta ggaaaatcta
     3241 cttattttat caagtaagaa aaatagaaaa cgctaatttc ttcgtgtctc ggatacattg
     3301 aataaaataa gtattctttc tcgtttaggt taaataccaa taagaaagga tagttttagc
     3361 agatggagtt catttttagc aagaaatatc cgtaaaacat gtgctttttc agaaaaaatg
     3421 gaagatttct tttcctatta attttttttg ttataatggg taggaaatga aaacttgcca
     3481 agaggagtaa tacaagatga ataatactta cccagacgac agtttgacgc tgcatacgga
     3541 tctatatcaa atcaatatga tgcagacata ctgggaatta ggtcgagctg atttacatgc
     3601 tgtttttgaa tgttactttc gagaaatgcc ttttaaccac ggatacgcgg tttttgcagg
     3661 attggaacgg ttagtcaatt atttagagaa tctgacgttc aatgattccg atattgctta
     3721 cttgcgtgga ttagaagtct atcctgaagg ttttttggag tatttacaaa attttgaatt
     3781 taaagcaaca gttcgttctg ctcgtgaagg agaactagtt tttgcaaatg aaccattaat
     3841 ccaagtggaa gggcctttag cgcactgcca attagtagaa acagcactgt tgaatatggt
     3901 caatttccaa acgttgatcg cgacaaaagc agctcgtatc aaatctgtca tcggagaaga
     3961 tccactattg gaatttggaa ctagacgggc acaagaactg gatgcagcag tctggggaac
     4021 tagagcggca tatatcggcg gagcagatgc aacaagcaat gtacgtgctg ggaagatatt
     4081 tgggatacca gctagcggga cccatgctca ttcgttagtc caatcttatg gaaatgatta
     4141 tgaagcattt atggcctacg ctaaaaccca taaagactgc gtgttcttag tcgacactta
     4201 tgatacgttg aaatcaggtg tgccaagtgc gattcgcgtg gcgaaagaat taggtgacaa
     4261 aatcaacttc caaggcgtac gcatcgacag tggagatatg gcctatatat ctaagcgtgt
     4321 tcgggaacaa ttagatgcag ccggatttac ggaagcaaaa atctatgctt cgaatgactt
     4381 ggatgaagca acgattttga atttaaagat gcaaaaagct aaaattgatg tatggggtgt
     4441 tgggacaaaa ttgatcacag cctatgatca gccagccctt ggtgcagtat tcaagctagt
     4501 atcgatcgaa gatgatgaag gaaaaatgat ggatacgatc aagttatcaa gcaatgcaga
     4561 aaaagtaact actccaggga aaaaacaagt gtggcgcatc actcgaaatt ttgatgggaa
     4621 atctgaagga gactacgtga cattatggga tgaagaccct cgagaagaag aagctatctt
     4681 catgttccat ccagtgcata cgtttatcaa taaaactgtt cgagacttta cagctcgccc
     4741 agttttacaa gatatcttta tcgaaggaaa aagagtttac gaattacctg cactaaacga
     4801 aatcaaagaa tatacaaaag aaaatctaga ttcactatgg gaagagtata aacgagactt
     4861 gaatccgcaa aaatacccag tcgatctgtc tactgaatgc tggaaccata aaatggctat
     4921 catggaacga atgaaaaaaa gcgtagcaga acttcataca gaagcatagg aggagaataa
     4981 atgagcctgc agacagaaat tatcaatgaa ttaggtgtca aaccaacaat tgatccaaaa
     5041 gaagaaatac gtaaaagtat tgactttatg aaagcatatt tgaaaaaata tccgtttctt
     5101 aaaacttttg tactaggtat cagcggcggc caagattcta ctttagcagg ccgtttggcg
     5161 caactaacga tggaagaaat gcgtgaagaa acaaaagatg aaaactatca atttatcgct
     5221 gttcgtcttc cttatggtga acaagcagat gaagaagatg caaaagctgc tcttgacttt
     5281 atccagcccg atgttagtct gcgagtaaat atcaaaccag ctgttgatgc acaagtacaa
     5341 gtattaagcg aagcaggtgt gggaatcagc gattttaaca aaggaaatat caaagcacgt
     5401 caacgtatga ttacgcaata tgccgtagct ggagaaagag caggagccgt attagggaca
     5461 gatcatgctg cagaaaacat tactggattc ttcacgaaat ttggtgatgg tggagccgac
     5521 attttgccat tattccgttt ggacaaacga caagggaaac aattgcttca agcattgaat
     5581 gcaccggaaa aattatacac aaaaattccg actgcagatc ttgaagatgg gaaaccaatg
     5641 attgcagatg aagtcgcact aggtgtcaca tataatgaaa ttgatgatta tctagaagga
     5701 aaagaagttc ccgctcaagc acaagaaaaa atcgaagcat ggtggaacaa gacacaacat
     5761 aaacgacatt tgccaatttc cgttttggat gatttctgga aataacaata aagcaaaaac
     5821 agccgtccct tcatagggat tggctgtttt tgttttataa aatagaaaag aaaaatatca
     5881 gctgtagtaa acttttaatt tgatatcctg atcgtaatta ccgaatcctt taccaaataa
     5941 agtacagccc cccacatgtt tggcatcgtc tttcacttcg attcgcaaat gccaagtgtc
     6001 ttgctcttta gggatgtcgt tgatcgtgat gtttgacaag ggttctccat tcatattcgt
     6061 caagtgtttg gtaattcgga tcgtcttcag caagccgtat tgattaagat tatctggata
     6121 ccaatcaggc gtatatttac cgcggatatc agcaaaatct ccagggctcg tccatgttcc
     6181 tagctccaca ccatttaggc tgaaagtgat atctgaaggc caattgtcat ttgaaaaagg
     6241 aaattcagaa gaaatttcca cagaaacttc caacatctcc aatgtatcct ctgtatttaa
     6301 aaaattaggt gcctgatact cgacaaatcc gctggtaaac cagagaatgc gggcatccat
     6361 ccttcgagga tccataaaat agcgtggttc atccactttc ccgataaaat catggatcgt
     6421 agctaaaccg catgttggtt caattgcata atttgtatag tgaccaatag gtatcgatgt
     6481 ttctttcgta tcaaaagcat tgaatatttt ttctggaaaa ctgatatcga ttttatcgac
     6541 acgaaggctc gaaatttttt gttgtcctac tttttctgtt ttgatgatgt ttgcttcctc
     6601 tagctttttc acgtgcatcg tcgtgatagc actgctcact ccaagttctt ttgccaaatc
     6661 tttgacattc atcttttttt tggaaagtag ctggatgatc ttgatccttg ttttactagc
     6721 taatgcttca tataccggta aggattcttc gtttatttgt agttgcatat atatcacctc
     6781 ttaaaaggtt tttcttatat attaacatat ttgttaataa aagttatatt aaattatttg
     6841 ttttaatcat tattaatatt ttggttaatt aaaatcgata aaaaataagt aagtttaaca
     6901 tttatattat tgacaacgct ttcaacgagg tgtattattc atgtataaat taaaggaggt
     6961 cagttatgga agccacaatg ttgattagta gtgtcaagcg tgtaggcaaa gttgatccgc
     7021 ggttgtacag ttcatttatt gaacatatgg gacgagcagt atatgaaggg atttatcagc
     7081 cagatcatcc atcagctaat aaaagtggat ttcgccaaga cgtcattcac ttggtaaaag
     7141 aattgaacat tcccatgatt agatatcctg gtggaaattt tgtgtcagga tttcgttggg
     7201 aagacagtat tgggcccata gcaaatagac ctagaaggtt ggatttagct tggcggacaa
     7261 ttgaaacgaa tgaagttggg attcatgaat tttataactg gtgtcagcat gtgggggccg
     7321 aaatcaatat ggctgtcaat ctgggtactc gtggagtaga tgcagctcgt aatttagtcg
     7381 aatattgtaa ttttcctaaa ggaacttatt ggagtgattt aaggcgttcg aatggtcaag
     7441 agagcccttt tggaattaag acatggtgtt tagggaatga aatggacggt ccttggcaga
     7501 ttggacataa aacagcggaa gaatatggac ggttagcaga ggaaacggct aaggcgatga
     7561 aactcgtaga tgattcaatt gaggtggttt tatgcggaag ttcgaatcgg caaatgccta
     7621 catttggtga ttgggagtta acagttcttg atcaagcgta cgatcaaata gattatttgt
     7681 ctctgcatca atattatggc aaccaaaaaa atgatctttc tcattatctt gcacgttcct
     7741 tagacatgga tcggttcatt tctgaagtga cagcgatgtg cgatacagtc aaggcgaaga
     7801 aacatagtaa gaagcagatc aacctgtcct ttgatgaatg gaatatttgg tatcattcag
     7861 cggaacaaga tgagcaagca gcgccttggc agattgctcc tcctttacta gaagataact
     7921 ataattttga agatgcatta ctattaggct gtctattaat cactttattg aaacatgcag
     7981 atagagtaaa gattgcttgt cttgctcaat tggtaaatgt tattgcacca atcaggacag
     8041 aaacaaacgg agaagcttgg agacagacga tctattatcc atttatgcaa gctgctgtgt
     8101 atggacaagg agaggtgtta agcccacaaa ttcgttcagc aaactatgcg acggaagatt
     8161 ttgaagaagt gccgtattta gaaagtatag ccgtaataaa tgacaaagaa cttgtcatat
     8221 ttgcagttaa ccgtggagaa gaagaaatga atctaactgt tcacttgtct gaattatcat
     8281 tagagggagt gattgatttt tctgaaatga gtggatttga tacaaaagca actaatcttc
     8341 ctaaaagtga acaagtaaaa ccaagtcatt caacaaatgt aaaagctgaa gatgggaaaa
     8401 tcaattgttt attgaaaccg ctatcttgga atgtggtcag atgtcgttta tcagatgaag
     8461 gaggaacaaa aaatgaaagg taaaaaatgg ttaattggct ctttgttggt agctgcaaca
     8521 ggtattttag gagcttgcgg tggcggaagt tcagcagaat ctgattctga taaagaagtg
     8581 actttgactt tttggaatgg gtttactgca tctgatggtg aaatattgca agaaatcgtt
     8641 aatgaattta ataaaacaaa tgataaaaac atcaaaattg aaatggatat catgacttgg
     8701 gccaatttga atgaaaaact tcctacagcg atttcttcta aaaaagcacc tgactttatt
     8761 gcattaaatt atccagattt tgcacagtat gtagaaaacg gagcggtaca accattagac
     8821 gatttctggg aatatgatgg agtagataaa tctgatttta aagaaacggc tattgaatta
     8881 ggtgaagtag aaggtaaaca gtattttgtc cccatgcagg tacaaggcat gtatatgtat
     8941 tggaataatg atttgtttaa acaagctgga ttagatactg aacaaccacc tactacttgg
     9001 gaagaattga cagaaatggc accaaaatta actgatagta gtaaaaatgt atcaggtttt
     9061 gtcttcaata aggatggaac tgcaccgtta tataactgga tgttagcaaa tggcggtaaa
     9121 ttagtgaacg atgattatac taagtctgaa tttgcgtctc ctgagaacct taaaacgtta
     9181 aaagcgatcc aaaaaatgat ccatgtagac aagactggtc cagaatcgat ttctggcgcc
     9241 gaaatggaca atttaatgaa tgctggtcaa ctagccattg aaatcaatgg gccatggtta
     9301 aataacggat tgaaagcaaa cgagattgat tataatgtga ctaccttacc acaagggagt
     9361 gattcagaca aaaaaacagc tattttagat ggcgtaggat atgccattcc ggcaagtaca
     9421 gataataata aaaaagaagc tgtctatgag tttttgaaat attggaatac aactgaaatt
     9481 ggtaaaaaat ggagtatcga aaatggtttt cctgcctact tggattcagt agcaaaagat
     9541 gaagaagtaa aaaatgatcc tattgtatct gaattatcga aacaaatgga ctatgcagag
     9601 ccatatttac caggttttac ccaaatctct gcgatcaata acgatatcat caatccactg
     9661 attgaaaaat tattagcagg agaagatcct gagacactca tgaacgatgc agatgatgcc
     9721 atcaatcaat tactgcaaaa atagtacaga actttataca ggatagactg ttggcaagat
     9781 acagatatct gaatgatcga cctagtattt gcagataggt gttattgcaa aaatcacagc
     9841 gattgttcgg atatcttatt cagattcgtt taaaccaaga ttgtatattt tatcaaaatg
     9901 aaagatgagg tggactatat gaacggattc accaaatatt ggaatcgtcc tgatcgtgct
     9961 gcgtatttgt ttttaactcc atctttttta gttttattat tatttactgt cataccgctt
    10021 ttaggaacat ttgctattag ttttacagat ttgaatattt tctttacatc aagaaatttt
    10081 gtaagttttg aaaatttcac acgtatattt agtgatgaac gagcagttaa ttcgttgatt
    10141 cattctttat atttcaccgt actcgaaacg ccaacacaaa ttatcgttgg tttattagca
    10201 gcagtagcat tatctaaaaa taccagattc aatcgttttt gtcgttcgac attttacatt
    10261 ccagttattt gttcattgac gtcaattgcg attatttttt ctatgttgct tgatccgaat
    10321 gtaggggcga ttccttactt gttttcacaa gtcggcttgc cgatccctca attttttcgc
    10381 gatccaaccc ttgctatgcc gacggtagcc atcatgactg tttggaagaa ttttggtgtt
    10441 actatgacga ttctcttgac tgctattcag ggaatctctc cctctttgta tgaatctgct
    10501 caaatggatg gtgcaacaaa tcgacagcag ttttttaaca ttacgctacc tcagatcgtt
    10561 ccctccttag gtttttgtat tttaacgaat ctgattggtt cgatgcaagt gtttgaccaa
    10621 gtatatgttg caaccgctgg tggcccacaa tttaagacag aaacagctgt tcaatatatt
    10681 tacagtcgag gatttacagc accttatgaa ttgggctatg catcagcact gtcttcggtt
    10741 ttgtttatca ttgttgcaac tatagctatc tccactaatg tgtacatgtc acggaaagaa
    10801 aaacaaatga aataggaggg aaacctgtat ggcaccagaa agctcgatta cagttactgt
    10861 gaatagtcga aaagcagtta gtggtaaaaa aatcattcga acgattagta tgctagttat
    10921 attgttggtt attgcgtttc cttttttgtg gctgattatt tcttctttta aacacgaaaa
    10981 agacattatc tcatttccgc ctagaatatt tgcagatagc tataccctag ataactatat
    11041 taaggtttgg acaacgatcc ctttattaga ttacatcaaa aatacagtga tttttgctgg
    11101 agggacagtg atcacttctg tattctttga ttctcttgcg gggtatgcat ttgctcgtat
    11161 gcgtttcaaa ggaaaatcag ttttgttcta tttcgttttg ctgacaatga tgatcccatt
    11221 ccaagtcttc atgattcctc tatttattga agtcaatttg cttggcatgc ttgatactta
    11281 tgcaggtttg atcattcctc gtatgacaac tgcatttggt atcttcatga tgcgttcctt
    11341 ttttattact ttaccagatt ctttggaaga ggcagcacgc attgatggat taagtgagtt
    11401 taatattttt ttgaaaatca tgttgccatt gagcaaacct actcttttat cattagggat
    11461 tttcacgctg atgaatagtt ggaatgactt gctttatcca ttgatattga caagtagttc
    11521 aaaaatgaga actttaccag cagggttagc tttatttaca gggcagaaca tttcttttta
    11581 tggacctgta atggctggaa cggttatttc aatgctgcca ttactagtgg tttatatttt
    11641 tgcacagaaa tattttgttc aaggaacagc tatgtctgga atgaaagagt gaggaagatg
    11701 aaaatagttg agactaaact ttatggacag ttgattgtgg tttctgatta catcttatta
    11761 ggtattctct gggtaatcgt gtgtttgcct gttattacta tcgtgcctgc tacatgtgct
    11821 gttttttatg taatggcgca atggaaaggt gagggatcag gaaatatcat ttattttttc
    11881 tttcaaggaa taaagcaaca ttttggtaag aacttgttcc ttagtctgtt aacgatcatt
    11941 atttattttc ttgttaatca attattagaa gaacagacaa tcgtactttc aattagtgga
    12001 tatgtagttt tacttattta ttccatgttt cttttatcct ggatatatgt gatttctgaa
    12061 aacaaagata tcacattttt acaaattttt gaacaaagtg catggaaagt attttttgat
    12121 tttttgagaa atattctttg ttgcgtattg ttattaggat ttgtactgct catatttctt
    12181 ttccctcctt ttatttttat ctttgctggt ggcgtatgga aacttgtatc tattatcttg
    12241 actataggaa atgaaaggta gggaaagctg ttggacgaag aagtgaaagt aaaaatcaga
    12301 gaggatttta cggtaggaga gatcagttct cggctgtttg gctcatttgt tgagcatatg
    12361 ggaagtgtcg tctatacagg gatttatgaa ccgggccatg aaacagctga tggtgacggg
    12421 tttagacaag atgtcctctc ttatgtaaag gaattagaat taggtgtcat tcgctatcct
    12481 ggaggaaact ttacatctgg ttatgattgg gtggatactg taggtaggaa agaacaacga
    12541 ccaaaaaaaa ttgatttagc atggcgaggt atagaaccaa attcttttgg actccacgaa
    12601 ttttttaaat ggatcagtca tgtacaagcg aaaccgatat tcacggtgaa tttaggaaca
    12661 aaaggaattg atgaagcaaa aaatttagta gaatattgta attttccaaa aggaaccttc
    12721 tggagtgatt taagaataaa aaatgggcaa aaaaagcctt ttggcgttaa attatggtgt
    12781 ttagggaatg agctagacgg tccatggcaa gtgggggcta agagtgctta tgaatatggt
    12841 aggttagcaa atgaagcctc aaaagtcatg aagttagtgg atgattcaat tgaaacaatc
    12901 ttagttggaa gctctacgcc acgtttacca tcgtaccctg aatgggatcg tatcgcatta
    12961 gaagaagctt atgagaatgt agattattta gctttgcata actatattga taagtatgat
    13021 gaagaagact tgactaaacc tcctaaaaat gagcgtgatg atacagcgac ataccttgca
    13081 aaagcttatc gattcgatcg tcaaattgaa gaaatcgttg ctacttgtga ttatgtaaaa
    13141 ggatatttga gaagtgagaa aacaatgtat ctagcgtttg atgaatggaa tgtacatgcc
    13201 caaccggaaa aaagacatag ggattttgaa gtcggtagtc ccatcgattg gtgttacttt
    13261 actatggaag atacattatt atttggttca ttagggctgg ctattttacg acacgcagat
    13321 cgcgtaaaga ttagttgtca atcattgtta gtaaacacaa tacctttaat actgacagat
    13381 gctaaaggac aagcatggag gaatccaact tattatgtga tgcaacactt atcgaaatat
    13441 gctaaaggtc aagtattgaa acaacaaatc atttgccctg tttatgattg tgaaaagata
    13501 aaaaatgttc cttatgtgga cagtgtgatc gtagataacg gcaatcagct gactattttc
    13561 ttgatcaatc gcacaaatga acaacaaaag ataacgattg aacatgattt caatttgcta
    13621 gatcaaggag agcactacac attaaccagt agcgatctgt tggacaaaaa tacacgtgaa
    13681 gaaccagaaa aaataaaacc tgaaataaac tatttttcaa tagaaaaaca tgacgaatta
    13741 aatgtaggga tagaaaaata ctcttggaat gtaattagaa taaagaaaat gtgaggaaat
    13801 ttgttatgat gaagacactt tataataatc ctatcgttcg gcaacgggca gatccttggg
    13861 tatataaaca tacagatggt tattattatt ttacaggatc ggttccaggc taccaagtga
    13921 ttgaattaag aagggcaaag tcactgaatg aattagaaca tgctgcaagc ctgattgtat
    13981 ggcaggctca tgaagaagga ccgatgagtg aattgatctg ggcacctgaa gtccattata
    14041 tcaatggaaa atggtatatt tactttgctg caagcaatca taaaactata cgtgatgaat
    14101 ctcatcatca ccgcatgttc gtacttgaaa atcagcatat agatccaatg aatacagatt
    14161 ggatagaaaa gggacaaata aaaacgaaaa gggaaacttt cagtttagac ggaacaactt
    14221 tctcttctca aggaaagcta tattatgtgt gggcccaaca agatcctgaa attactggga
    14281 attctaatct ttatctatcc gaaatggata atccttggac gttaaagggg aagcagatcc
    14341 tcctttctat acctgagtat gaatgggaaa aacggggttt ttcagtaaat gaaggtccag
    14401 cagtccttac tcgcaatggg aaaatattca ttacgtattc tggtagtgca acagatgaaa
    14461 actatgccat gggcctgctg tgggctgatg agcacaagaa tcttttatta aaagaatcat
    14521 ggcataaatt atcggagcca gtattcgttt cttctgaaaa aaataagcaa tttggtcctg
    14581 gtcacaactc gtttacggtt tcagaagacg ggtgctttga tattttgatc tatcatgctc
    14641 gaccagagaa gaaccaaaaa ggggatccgt tagacaatcc taatcggcac gcgaacgcgc
    14701 aatgctttac gtggaatgag caaggatttc cagaatttgg tgaacctgtt ccgtatgcct
    14761 tatgagcaca agaaaccttg atttttgctt gaataaacct tgcagactcg ttatgatata
    14821 tgtacgtaca aataaaaagg ggcaaggcaa atggcaaaat atcaagatat tgcagaagag
    14881 ataaaacaaa agatccttcg tgaggaatac gaagtaaata caaccattcc ctcagaattc
    14941 aagctgcaag aaatatatga cgtcagcaga catacgattc ggcaagcaat cgctgtgttg
    15001 gtaaatgagg gctatctgcg taaagaaaaa gggtcgggca catatgtaga tgacagctat
    15061 aagaaagaaa aaacaccatt gaacagaaca aaaacgatcg gtgtcatcac cacatatctc
    15121 tctgattata tttttccgtc gattatccga ggaatcgaaa atgaattacg agaatctggg
    15181 tattcattgc ttttagccag tacgaataac gatcacgaat tggagaaaga gtgtttgaag
    15241 aaaatgattg cttatggggt agatggtttg atcgttgagc ctacgaaaag taatcagtat
    15301 aacccaaact tagcacttta tgttgctttg agagaacaag gcattccgat ggtgatgatc
    15361 aatgctgctt atgaagagtt aaatgcctct tatatttgtt tagatgatgt gaaagtcggt
    15421 tttatggcag cagaatattt gataaaaaaa ggacatcagc gtctgttgtt catcacaaaa
    15481 atcgacgatc ttcaaggaaa atacaggatg aaaggtttta tcagcgcgtg tgaacagtat
    15541 aatgtgcgtt ttttgacgga agatatcatt actttcacca cggaaacacg agaaacaatc
    15601 gctgaaaaaa cagtacaacg gttgaaagaa gaacaagctt tagatggcat catttgctat
    15661 aacgatcaaa tagctagttt aatgctagat aagttgacag aaaatggcta tcatattcca
    15721 gatgacctat cgatcgtagg aacagacgat tcttctttaa gccatttagg gaataaaaaa
    15781 ttgacaacat tgacgcatcc aaaagaaaag ttgggacaag atgccgcacg gtggatcgtc
    15841 acaacgattg aaaaaggaca ggcacagcca aatattctct atgaacctgt actagttgaa
    15901 cgggaaacag tgaaagaaaa cgaaaaataa gagacgggtt tttaccgcct cttattgctc
    15961 ctacggttac tcgtcgcaat gtttgtgaga gcttttgtca tcgctcaagg ctgaacgttt
    16021 ctttcaccac ctctccacta aacggtgttc tcctaccaac tggtcacaag tcacaatatt
    16081 catcaaacat gagaagctgt tttttgaaat tgttccttgt gactggaaac taagcggttc
    16141 cttcttattt cttgaagctg accacttctg tcacaacctc tttaagaatg atggatagaa
    16201 atacgtttag gataagtttt tttagatgca ctttgttcga tttcctctaa agatttccca
    16261 cgagtttctg gtacgatctc ttgaataaat aaaaccccta aaaagcagat gatcccaaaa
    16321 atgccgaaaa cgatttcttg agacatagaa gcagtcatca caggaaaaag caagccaact
    16381 aggaaagaac caatccaatt aaaagaagag gctaggccag atgctcgccc gcggacagct
    16441 agtgggaata tttcgcctac gattacccat gtcagtggag cccatgtgca agaataaaat
    16501 gcgacataaa tacataaaaa caagagaatc aacaacgaat ttgtatgagc atccagtaca
    16561 gttcctaaaa cagctggtag gatgaaagaa agtcccatca cgctaccgcc tattttcaat
    16621 aaagttcgcc gattgaactt gtctgcaata accaaaaaga gcaaagaacc agcaactaaa
    16681 atgattcctt ggatgatcgg ccacatcaat gcatcgctcg ctgcatgccc agtcgctttt
    16741 tctacgatca gcggaatata gtaaaaaata gcattagctc cttgaaattg ctggaatact
    16801 gctacaccaa taccggcaat gactaaggaa cgatattttt ctaaaaacaa agtatgccaa
    16861 gaagttccgg ttgtcttttc ttgtttgaca ttcagctgga tttgttcgaa ttcctgttca
    16921 gcttcttttg gtgtccgaat atagttcaat actctttttg cctcttccaa gcggccagat
    16981 tgcataagaa aacgcggtga ctctggtaga gctaaaacac cgaaaaataa aatcagcgcg
    17041 gggacagcag cgagccctaa catcacgcgc caagccatcg tttcaggcaa cccttttagc
    17101 aaataatctg caacatagga aaggagcatg ccagaagcaa tcatcacttg attgatccct
    17161 gacaatcttc cccttagtct agcaggagcc atttcagaca tataagcagg aactagggca
    17221 gatgcagcgc caacagctaa acctagaaga atccttgctg caatcagaaa ataagaacca
    17281 ttgtgagggg cagatgcaga aaggacagaa ccagccataa aaataagtgc agaaagaaga
    17341 atcatttttc gtcgtccaag cttatcagaa agaagaccag ataaagagcc gccaaggatc
    17401 gcacctaaca tgaccgaaga cgtgatccat cctatcaaag aagctttacc tgccaaaccc
    17461 caatcatgct gtaaaaaagg taaggcgccg gtcataacac caatatcata tccaaataga
    17521 atgccgccaa atgcgccaaa aaaataaata aacgatgagg aaactttttt ttcttttttc
    17581 ataagtattc tctccttcta gaatttcgag aaggcggttg atcagatttt gtaagcaatt
    17641 tcattttata acttatgtac gtacaaaaca agtgtttttt attgtattct tgtaaataac
    17701 tgcttgacat gtacgtacaa aagtatacaa taatcattgt aagcgctact aaaaagacgg
    17761 tttgatcagg tttgacgaga aggagggagt taaatcatta tgaaggaagt aaatgcgcaa
    17821 agtatgttag atgtcataga tggtcgtaca tctttaggga ttgagtttgg atcaactagg
    17881 atcaaagctg tattgattga ttcatctttt actcctattg ccgctggtag ttatgagtgg
    17941 gaaaacaaac tagaaaatgg tatctggacc tattcattag aagatatttg gaaaggactg
    18001 cgcgtcagtt accgaaaaat ggcagcagaa gtattcgaaa tctatggcga gaatcttacg
    18061 acaatcgggt cgattgggtt cagtgcaatg atgcacggtt atctagcatt tgatagcaag
    18121 gaacagttac tagtaccatt tcgtacttgg cgaaatacaa tcactgaaga agcagaacga
    18181 atattaacgg aaaaatttca atacaatatt ccgcagagat ggagtatcgc tcatttgtat
    18241 caatctattt tgaataaaga agaacatgtg aaagaaatta attttttgac aacgcttgca
    18301 ggttatgtcc attggcagct tactggtgag aaagtactag gcgtaggaga tgcatcaggc
    18361 atgtttccta tcgatccttc aacgaaaacc taccatcaag aaatgaaaaa tatttttggt
    18421 cagttaatcg aaaaagcgca cgtagcgatt gacttggatc acattttgcc taaagttcta
    18481 gtagcaggag aagaagctgg caagttaagc gaatcagggg cttatctgat cgaccctacg
    18541 ggaaatttga gaccaggaat cccggtttgt ccgccagaag gggatgcagg aaccggtatg
    18601 gtcgctacga acagtgtagc caaagggaca gggaacattt cggctggtac ttccgccttc
    18661 gctatgatcg tattagaaaa agaactagaa aatgtccatc cggaaatcga catggtcact
    18721 acaccttcag gagaactagt aggcatggtt catacgaata actgctcttc ggatatcaat
    18781 gcttgggtca gtatttttga agaatttacc aattctttgg gtatggagat aagcagagat
    18841 aaattattca cggtattatt caacaaagcg ctagaaggcg atacggattg cgggggatta
    18901 ctttcttatg gttatttttc cggtgaaaac attacaggag taaatgaagg aaggccatta
    18961 tttgtacgta caccaggtag ccgttttgat ttagcgaatt tcatgcggat ccatttagcc
    19021 agtgcttttg gtgcaatgag aatcggaatg gatatattga aatctgagaa tgtccaaatt
    19081 gatcgtttag tggggcatgg ggggattttt aaaacaccag aagttggaca gcggattcta
    19141 gcatctgcta tggaggcacc agttacagtg atggacacag ccggagaagg tggagcgtgg
    19201 ggaatcgcgt tattagcagc ctatatgatg gatcgacatg ggaagtcttt agaaaacttc
    19261 ttggcagaag acgtgtttgg acaagaccaa ggaatcacca tcacaccttc agcagaagaa
    19321 atagcaggct atcagatatt tatgagacgt taccgtgagg ggatcgacat tgaaaaaaca
    19381 gcgattcatc aattgcaaga aaaaggagtg atagaaaatg ctggaacaac tgaaagaaga
    19441 agtataccaa gcaaatcttg acttgcctaa acacggactc gtcaaatata catggggaaa
    19501 tgtgagcgct tttgatcctg aaacacgata tttcgttatc aaacctagcg gagtcagtta
    19561 tgaagagatg acagcagacg acatggtcgt tgtggacttg gacaatcata tcattgaagg
    19621 taaactcaat ccctcttcgg atacgcctac tcatgctgtt ttatatcgtt catttcctca
    19681 aatcggagga atcgtccata ctcactcgac atgggcgacg atctgggcac aagcaggatt
    19741 ggatgttcct gcgatgggga cgacgcatgc agatactttt tatggatcgg taccttgtgc
    19801 tcgatttttg acacaacagg aaatcgacag cgggtacgaa tatgaaacag gaaaagttat
    19861 catagaaaca ttcaaagaac gcaaaattga tcccttagca attccagggg tacttttaca
    19921 cggacacggt ccatttactt gggggaaaga tgcgcaaagt gcggtaatga acgcagtcgt
    19981 actagatgaa gtatgcaaga tgaatttatt taccagacag ctcaattcat tttcagaaga
    20041 gctgcctcaa cggattttag ataaacatta tttacgaaaa cacggaaaaa atgcgtatta
    20101 cggacaaaac aaataaaagg agtggctaat tatgttgaat attggagaaa aagaattttg
    20161 gtttgttgta ggatcacagc atttatatgg ggaagaagca ctgcgagaag taaagaaaca
    20221 tgcacaagaa atggtcgatg aactgaatga aaatggccaa ttgccttatc caatccgcct
    20281 tcaagaatta gcagttactg ctgatacgat cacaaaaatc atgaaagagg tcaattatcg
    20341 agaagaagta gcaggtgtga tcacttggat gcatactttc tcaccagcaa aaatgtggat
    20401 ccgcgggaca aagcttctac aaaaaccatt gcttcattta gctacgcaat ataacgaaag
    20461 cattccttgg aaaacgatcg atatggattt catgaattta aatcaatccg ctcatggtga
    20521 ccgcgaatat gggttcatca atgctcgatt aaataaacaa aataaaatcg tcgttggcta
    20581 ctggaaacga cctgagatcc aaaaagagat tgctgattgg atggatgttg cagtagccta
    20641 taatgaaagt tttggaataa aagtggctcg ttttggtgac aacatgagaa acgtgggtgt
    20701 cacagaagga gacaaagtgg aagcacagat tcaatttggt tggactgttg attattttgg
    20761 tatcggagat cttgtacaag taatcgatcg cgtatcagac gaagaagtcg atcaattatt
    20821 tgaagagtac aaggaattgt ataccttcga ctatggtgat tatgaagaaa agacatggga
    20881 ggaacatgta aaagtacaag ctcagcagga aatcggtatt cgccgtttct tagaagaagg
    20941 aggctataac gcttttacga ctaattttga agacctgtat ggaatgaaac aactacctgg
    21001 actagctgta caacgtttga tggcagaagg atacggtttt gctggggaag gcgactggaa
    21061 aactgcagcg attgatcggt tgctgaaaat catggctcga gggaaagata caggattcat
    21121 ggaagattat acttacgagc tagctagtgg ccaagaagca atcttggaat cccatatgat
    21181 ggaagtagat ccgacgcttg ctgcgacgaa accaagaatc gttgtttctc ctttatcaat
    21241 gggagataga gaagatccgg cgcgtcttgt atttgatggg aaagcaggag aaggggtagt
    21301 cgtatcgatg gcagatttcg gtacgcatta taaactgctg atcaatgaag tagaagcctt
    21361 tgaaccaacg actgaagcac ctaatttacc tgttgcacgt gtgttatgga aaactaaacc
    21421 gaatttccat gaaggtgtcc attcatggat tcaggcaggt ggcggtcatc ataccgttgt
    21481 ctcgctaaat ctaacaacag atcaaattga aacgtgggca aaattagttg agttggaaac
    21541 agtggttatt agataaaaag aggttgtgaa aaagccactt agttttaaga aataagaaga
    21601 gattgacgaa atttgctttt caactttttt gaaatttagt cttattatca atgtcagcta
    21661 acggaacacc atttatcaag atatctcacc ctcaatattt tgctctcgaa aataaggctt
    21721 atggcagtaa cttgtcatag ccttattttt atcactcagc ctactctgct ctaactgctc
    21781 tttttgcttc taggaagtca aatggtaaac agttgtctaa ttgcagaaat ttcgagatac
    21841 ttgttagtaa ggagggggtt ttatgtatca cgttcatttg attgatgcta ttccagaaca
    21901 agagatagaa aagttacaag gggaatattt caaaataata gatgatttgc cagccgaagg
    21961 aatagttatt cgtagtacga aagtgaaaga tgaatggctt acaccagatt tgctagcaat
    22021 ttcaagagct ggtgttgggg tgaacacgat caacgttgaa aaagcttctg aaaacgggac
    22081 gatcgtcatg aatacaccag gtgtaaatgc taatgcagta aaagagcttg ttttgtgctg
    22141 tttgctctta tcttcccgtc cgataatcga agcaagtcgt atggtacaaa cactaacagg
    22201 tcctaatatt ttagaacagg cagaaaacaa aagaagtgct tatgtgggac gagaattgca
    22261 aggcaaaacc atcggtcttc ttggtcttgg agcgataggc acaaaagtag cgctaagttg
    22321 ctatagctta gggatggatg tcttgggtta ttctatccgc gatgcccagt tggattatgt
    22381 tcggcaagca gatttagaaa cagttctttc tacctcggat tacatcgtag tcatgctgcc
    22441 gttgacagaa gatacaaaag gattgatcga tcaggcgaat atagaaaaaa tgaaaaaaga
    22501 tgctgtactt atcaatgtag gaagaagtga aattgttgat aaatatgctg ttatgcaagc
    22561 attggaaaaa caacatctgg ctaaatactt gactgatttt ccagaagaag agttcttaga
    22621 aaatgaccgt atacttatgt tgcctcatct aggggatcga ctcaggaagc tttggcagac
    22681 agcggacgtt tggcagttga agccttaaaa gattatcttt tgttcggtac ggttagagaa
    22741 gcggtcaatt atccatccgc acgaatgttt ttccaagcgc cgtttcgatt cactatattt
    22801 tatcaaaaga aaaccaatat attagcagag atattttctc tgctgaatga gaacgagcta
    22861 gcgatcgcag atatatcgcg taaccataag aatgagcatg tctacatatt gattgacata
    22921 gacagtactg attttgaaca gctcaatcaa gtgaaacaac agctagaaaa aatttctgga
    22981 gtacgaaagg tccgcttatt aaagaaacca gaaaggccat aaagaaagag aatatttgga
    23041 tttcattaat tcttctttcc gttctttact ttcgaatttc gtacaactaa aataagagaa
    23101 gcaacattct tttattgata gaaatgaagg agcaagcatt tgaaaagata tataagcaga
    23161 ctttcgacca aacagaaaaa gattttaaaa ggaacagcag caatcatcag tatctttttt
    23221 cttgttgttt ttagtaattt atttttgcag tggtgtcaaa atgatctatc cgtagacctt
    23281 gcgttgaaat ttgctttttc ttggcatacg gaaaaattct tcttagcctg tctcgtcttg
    23341 ctgattattc ttatttttct gattgcacta gcaggatctg tccctttagg ttcccttacg
    23401 tatgtcgtag ccatcggtgt attaggtttt gcaaattata tgaaaatgag ttatcggcaa
    23461 gaaccgattt atccagacga tttgaagatg atcactgaaa tcggattgct gaaagatatg
    23521 accggcacta tgctgtttac agtgatctta gctgccgcag gaaccgtgat aggattgttt
    23581 tgctggtaca tgtttcgaag cctgaaaaaa ggaaggcgat tccaattgat ccgattaacg
    23641 acactgcttg tggcaattgg actgctaggg tatatcagta atttcaacaa tcccgataat
    23701 ttgttgcgaa aagcatataa taaaacggca ctatggatac cttacagcca gaaaatgaac
    23761 tactataaca ctggttttat cggtgggttt ttgtacaatc tgaaggtaga accaatggat
    23821 gaacctgaag gatattcgaa agaaaagatc aaagaaatca ctgaaaaata ccagaagcta
    23881 gccgatgaaa aaaataaaac tgtagaggaa gagtcgccga acatcgtgtt cgtgatgagt
    23941 gaaagtttct cagatcctag tcgattaaac ggcgtagaag tatctggtga gccattggca
    24001 gattattatg aagtagctga tcaaacatat agtggcaata tgctttctca aaactatggc
    24061 ggtggaacag caaatatcga atttgaagca ttgactggtt tttctatggc gttatttaat
    24121 gcgcaactca ccacccctta cacgatgctt gtaccaaaaa tggatcagct tccttctatc
    24181 gtttcagcgt taaacgcaca atcataccag acgacagcga tccatccata caatacttcg
    24241 atgtataaaa gagaggatgt ttatcagaca ttaggatttg accagttcat cagcgagcgt
    24301 acgatgacct atacggatac gatagagaat aatccatata tttctgatga gtcagcttac
    24361 aaagaagtaa tgacacttct caaggaagaa aaaacacctc agttcattca tttagtcacg
    24421 atgcagacac atatgcccta cgatggaaag tatgataatc tagactatcc tgcaaaaact
    24481 tcagatggaa gtgggacact tgacttagag aattatctgc aagatatttc ctatagcagc
    24541 acggcactga agcagttcac ggaagaattg aagaatcttt ctagaaggac tttagttgtt
    24601 ttttgggggg atcatcttcc agggatctat tcagatacga tccaagcaaa aaatgacaaa
    24661 caaacattgc atgaaacaca atttcttatg ttcgacagta aaggagaatt agaaaagcaa
    24721 acgactcacg atgcaatcac cagtccgttt tattttgcag cgaatctaat ggaacagacg
    24781 aatcagacaa caaatggctt ttatcagctg cttttatcat tagaacagga acttccggcg
    24841 tttgaacgag aactgtacta tcaaaacgga cagtggtata aagaagcgca attcaatcgc
    24901 tcgcaacaag agatttacga cgaatatcaa ttgatccaat atgacattgt cgcagggaaa
    24961 caatatagtt tagcagatgg tttctttgag cacgaataag aatggcgagc tgtatagaaa
    25021 ccattaaatt ttttcaagta cttgtcattt gaggataaaa acaagtagaa taaataataa
    25081 caacaatgga gaaagtagga aaacgaacat gttaccgaat attggctatt atgtgcagtc
    25141 catcgatcaa atcgtcaagg aaacagaagc aatcggagaa ggaatgcatc cgaaatatga
    25201 agtgatcaga aacgcaattg atgaaggaaa aacaagtgaa ctggacaaat cgactcttga
    25261 agagatcatc ctgttatttg aagaaggaac aaaaaaatat cgggcaatgc ttatgaaagt
    25321 cagtactttg cgtccaccag caaaagcaat gggcattcat aaaaattttg aaaaagctta
    25381 tctttcatat gtagcaggct gtgaagaaat gatccaaagt atcgatcttg aatcaggggt
    25441 agatgaagat ctattcaata catctgaagc aaaacaagac caagcgacag accaattgac
    25501 agctgccatc caaaaaatga ctaatttgtt gttgaaaaaa taaaagaaat ctttgataac
    25561 aataaaaact agagttatct aatataaatg taatattacc tttatgatag agtgatcaaa
    25621 atcctttatg ataatgacat acctataacc aacaacaacc ttgtacttac tcaaaatcga
    25681 gtaaatactc cttttccgtc gagtctaacc atctcggcgg ttttttattt gtagaaaaga
    25741 acatcaggat agacggaaaa gtcttgtcct gatgttcttt tcagatagta tggaaagctg
    25801 attagttttc tacattctgt acatattgtt caaagtagtt ctgtaaatcg gctttgagtt
    25861 tctgatattc ctcttcgcta tactctttat cttcgatttt cccatcaaag aaaggaatat
    25921 ttagttcttc ccgtagtctg tctaatacgt cttgctgatt catttcattc tccttttctt
    25981 actaacgatc aaaataaaca cagacacatt ctggtacata gacatctttt ttcagtaatt
    26041 ccagtaatcc tgtttcagga tgacggcgat ataatgtcag attatcggtg ttctgattgg
    26101 cacacacgac aaaattattg cttggatcta gtgcgaaatc gcggggaaaa tccccttctg
    26161 ttgagatgat ttgcttgttt tgaatgtggg aaccatcttc aaaaactgcg aatacagcaa
    26221 tcgagttatg tcctcggtta gaagcataaa ggaacttgcc atcatttgaa atgcggatag
    26281 ctgcccctcc attttcaccg tcgaattctt ctggcaaggt cgatattttc tgtaattctt
    26341 caaaagcacc tgtttcttta ttataagaaa gcacgcggac tgtgctgtct aattcaccaa
    26401 ataaataagc gaattgtcca tttggatgaa agacaagatg gcgagggcct gttcctggct
    26461 ctgcagtgaa ggtagttaca agattcaatt tgccgttttc ggacacatca taagtataca
    26521 cacgatctgt accaagatca catacgacta atcgttggtc aggagtcaga tccgtatagt
    26581 ggacatgcgc atgatcttgg tttttatgag gacctgtcgc ttcttgatgg aatattgaat
    26641 ctgctgcttt cagttcgcca tctcctaata tatggtagac atttacttca cctttatgat
    26701 agttcgcccc gtatagtaat tgacgtgctt catcaaccgc tacataacac aaaggagcgc
    26761 cagactctgt taccgcattc aaaaaatgaa aatccttatc gtacgcaccg gcaccaccta
    26821 atccatccac actagtaact gtgtaaagat tgccagcttt actttttgct acataggtag
    26881 ggctattttc ttcaacagct aatgaaaggt tggctaattc ttctttttct gtatcaaggt
    26941 cgatcgtata gattccttta ctttcgcgac gcgtataggt tcctaaaatt atcttttcga
    27001 tcatatgagc tgattcctcc taattgtaat gaaatagttt tcgtttttat tctatcacat
    27061 aattattcca cacggctaaa gaatgagaat gtgttatact gtttttgaaa gcaattaaag
    27121 gagagagtgc aatgattcag gcaaagatta cagaaattgg gacagaagca ataaacaagg
    27181 aagaacccat gctgattttg ttcgataaaa cagcaacagc tgccttacga aaatattcgg
    27241 tgattcaaga agtatcaaaa gacgaacctt tttctttgaa agcaggcgga acaattgctt
    27301 ttgatgatca ggtatatacg atcgattatg tcggtccgat ggccaatggg aatttaaaga
    27361 cagtagctca cgtatcattg atttttgatg cttgtccaga agaaggacaa attgcaaatg
    27421 gaatctatct tcatccgcat agactgcctg ctattggttt gggaagccaa attttttata
    27481 actaacagga ggaattatgg aaggaaaaaa taagaagaac agaccggtat cgtggttttg
    27541 gcgctggttt ttaaataatc aagtagtcac agccttactt gttgtattgt taatattgct
    27601 gatcgtcttt ttatttacaa aagtttcata tttgtttgca ccaatctggc aatttttagc
    27661 aatcgtaggt ctgcctatca ttttagcagg aatcttgtat tacttgatga atccggtagt
    27721 tgattacttt gaaaagcgaa aagtaccaag gctttacagt atcattgggc tgtttgtttt
    27781 ggttgttgca ctaatcgcat gggggagcgt agtgatcatc cctaagattc aggaacaaac
    27841 agtgagcttc atcagtaact tccctcaata tgtagatacg attgataata aattaaccga
    27901 gattttgcgg gacccgctgt tcaaccaatt tcgcgaacag ttggaaacag caggtgataa
    27961 gttcatgaat tcggcaggcg atatgatcca agatatatct aagagtacag ttcagagtct
    28021 tggaagtttt gtcggcgcgg tagcgactat tttagtggct ctgctgacga tgccgtttat
    28081 cttattttat ctattgaaag acggtaaaca attagcacct tattttgtca aatttttacc
    28141 gacacgcatg caaaaaccga cgttgaatgt gctgagtgaa atgaatagtc aagtttcttc
    28201 ttatataaga ggacaactaa ctgtggcttt tgctgtggca atcatgttta tgattggttt
    28261 tgcggtcatt ggtttagagt atgctgtcac gttggggatt attgcgggtt ttttaaacct
    28321 gatcccttac ttaggatcat ttttagcgat gattccggct atctttttag ccattgtagc
    28381 aggtccattc atgatcatca aagtactgat cgtttttgca gtagagcaga caatagaagg
    28441 acgcttgatc tctccattag tactagggaa tcagttagca atccatccag taacgatttt
    28501 attagttctg ctgacttctg ggaaattgtt cggaatcatc ggtgtgatct tggggattcc
    28561 tgtttatgca gcagctaaag tagtgattac tcatgttttt gaatggtaca caacaatctc
    28621 ttctctttat gaagaaaatc cgttgaaaaa gcagaaagaa taacaaaaag agacagatgg
    28681 gaaaatatcc aaggtacaaa tgctgctttg aagatttttt cctctgtctc ttttgtatgc
    28741 atttatctca atttttaaaa tgatttacct aaagcagcag ctttatctaa tgcattttgc
    28801 atcaactcat ctgcacggtc agggtcataa tcgattcctt cgatgaatag ctgatctact
    28861 tgatcgatgc ctacaaagtt taaaatacct tttacatatt gagaggcaaa gtcttgtccg
    28921 ttgtatacac cgccattaga ttggatgtgc agtgcttttt tacctgtcac taatccttct
    28981 ggcccgtttt ctgtatagcg gaacgttttt ccagctacat tgacggtatc gaaccaagct
    29041 tttaattttg ttgggatatt tagattccat aaggcgtttg cgataacgat tttatcagca
    29101 gcaaggaact gatcagtcaa ttcattgaaa cgagcaattt tttgttgttg actttctgat
    29161 aaggaagtga attctgcacc agcacgtaat gcttcccaac ctgataataa ttcttcatcg
    29221 atttccggaa tatcatcgcg ataaagttcc aaaacagtca cttcatcatc tggatgattt
    29281 tctttataac ttgttaaaaa agacgtcaat gccttaactg tgcgcgattc ttctgcagtt
    29341 aggggatgac ctttgactac taaaacagat gccatatgct taccttctcc ttttattgct
    29401 atgattgtta taactatgag aaaaagaaaa ggctatcaag tcatattgat aaggttgaag
    29461 aataaataac taaaaaaaga cagggtataa aagaggctgt gacacgattt tttgtcacag
    29521 cctcttttga atctaacaag caatattcaa gcaacactcg actgaagagc ttccatccca
    29581 ccaatgatat aaacagcacg aaaaccatta gcagtaagtt gatcggctaa tgcttctgat
    29641 cgcctgcctg tatagcttag aatataataa agtttctttg gatctagatt ttttaattgt
    29701 cttggcaaga tagcagcggg gatagaaagc gagtggctta agtggttttt tgtgtaatct
    29761 aaggtatttc ttgtatctaa aacgatgctg tcagcatcga gaggcaattt attgaagtct
    29821 tggacactaa ttgattgggc catttatctc atcctttgtt tagtatgtgg ctattttaat
    29881 gggcaactct cattaattca aatggaagga ttcctcttat cactttttta atgtatcgga
    29941 tgataatgaa aaaaactatg caaatgtctt gcaaaacagg taggaggtgt tataatgaca
    30001 cagcgtcata tgacaatgtg tcatcttgaa agaaaggagg atccatatgc cgacacagac
    30061 atttttccat ttgcctaaag aaaaacaaaa aagactaatc gaggctgcga gaatcgagtt
    30121 ttctagagta cccttaaaag aagcttcgat tgctaatatc gtcaaattag cggatatccc
    30181 tagaggcagt ttctatcagt attttgaaga taaagaggat ctttattttt attactttga
    30241 gacaatgaga cgtgacagct ctaaagatat gttacaacta atgaaagaat ccgatggtga
    30301 tctattcaaa ggattcgaat cctatttttc taaaatgatt tatgagattt tggaaggcga
    30361 gaatgcttct ttttatcgta acttgtttat gaacatggat taccgtgcct caagaaaagt
    30421 cgctcctcat acgagtcgcc aaaaaacgcc tcacgatcca cataaagaac acaaacaaga
    30481 tgcaaaagag ttgcttgaat tgatcgatcg gacgaagctg aaagtaaaaa atgatcatga
    30541 attggaatta ctgatccaat tagtgatgaa tactgtattt acaacaattg cgcattcgta
    30601 tcgcttattt tcaacatctg aagattactc agtagagcaa gcaatcagtg aattcaattt
    30661 gaaactttct tggctgaaaa acggagtata taaatagaaa gaagggaact agatgctaaa
    30721 aatcgtaaaa cggatctcct tgacttcagc aattgcagct gcagttttca tggtgatcca
    30781 agtactggca gatttatatt tgccgacact tacctccaat attattgata aaggggtagc
    30841 gcaaggagat gtcgattata tttggcatac aggttttgtg atgatcggct tttctttgat
    30901 cagtatctta gctgctatcg caaatacatt ttttgctact cgtgaatcac agaaactggg
    30961 taagcaattg cgtacggatg tctataaaaa atcagagagt ctgacaaaag atgcgtttga
    31021 taaatatgga acggcctctt tgatcacaag aacgaccaat gatgtgactc aaatccaaat
    31081 ggttacacaa atgtttttac gtatgatgat caatgcacca atcacgttga ttggcgcgag
    31141 catacttgct tatcaaaaag atcatcagtt gacaaaaatc ttcttagttg tcatcccagt
    31201 aatgattatt ttgatcggcg gaatcatgta ctttgctgtt ccattattca aaagtatgca
    31261 aaagaaaact gaccgtttaa acctggtatt tcgagaagga ttgacagggg tcagagtgat
    31321 tcgagcattc gataagacac gctttgaaga aaatcgtttt gatttagcga ataaagatta
    31381 tacaaatacg gcaatcaaag ttaatacgat cgtcgcattg atgatgccaa tgatgacatt
    31441 gatcatgagc ggtacaaacg tagcaatcac ttggtttggt ggacactaca tagcagatat
    31501 gactttggaa gtcggtaatc tgattgcatt tatgacttat gcgatgcaga tcttgatcag
    31561 ctttatgatg ctttccgcta tttttatcat ggtgcctcgt gcacaagctt cagctgaccg
    31621 tattaatgaa gtgttggatg aaaaaatcgg tatccatgac cctgaaaatc ctaaaacagt
    31681 ttcttttgct ggaaaaaacg caacattggc cttcaatcat gtcaattatc gatatcatgg
    31741 agcagaaaaa cttgcattgg aagatattga tttccaagcc aagtcgggcg agatcgtcgc
    31801 aatcataggg gggactggtt caggtaaaac gaccttagtc aatttgatcc ctcgattata
    31861 tgacatcgaa tctggttcga tccaaataaa tggaacggac attagtgaca tgactcaata
    31921 taatttgcga gaattaatgg gctttgttcc tcaaaaagca gttctgttct ctggaacgat
    31981 ccgagacaat atgcagtatg gtaagccgga tgcaacagat gaaatgattt ggaaagcatt
    32041 agaaatcgca caagctaaag attttgtttc agagatggaa gatggcttag atagccatgt
    32101 ggaacaagga ggaggaaact tctctggtgg acaaagacag cgtctggcaa ttgcacgggc
    32161 attagtcaaa acagcagata tctatgtgtt tgatgattcc ttctcagcgt tggatttcaa
    32221 aacggatgcc aatcttcgcc aagcattgaa aacgaatatg aaagaaagta tcaccgtttt
    32281 agttgctcaa cgagtaagta cagtaatgga tgcagatatg atccttgtgc tagatgaagg
    32341 aaaactggtt ggcaaaggaa cacatgaaga actattagca acaaacgaaa cttatcaaga
    32401 aatcgttcat tcacaactaa gagaggagga cttagcatga gtaaaacaaa cacacgtccc
    32461 tctcatggac cagcaggcgg tccaggacat ggtggacctg gcagaaatct cggtgtaaaa
    32521 ggtgaaaagc ctaaaaattt ctggggaacg gtacgccgat tgtttggcta tatgtctaaa
    32581 cgtatggttg cgatcatcgc tgtatttgta ctagcaatag cagccgtgat cttccaaatc
    32641 caaacaccaa aggtcttggg gaaagcaacc acagaaatat tcaaaggtgt aatggctggc
    32701 gctcaacaga tgcaacaagg ccaacaagta acaaaatttc cgatagactt tgataaaatc
    32761 gcgcagatca ttgcgacagt aatcgtgatg tatttgattt ctgctgtatt caacttttta
    32821 cagcaattta ttatgacacg tgtttcccaa cgaacagttt atgaattacg tcatgatttg
    32881 gaagccaaaa tgaatcgtgt accgatttcc tattatgata cacatacaaa tggggatatc
    32941 atgtctcgag caatcaatga tatggataac atcgcaagca cgctgcagca aaatttgact
    33001 caatttgtaa ctagtgcagt cacattgatt ggggtcatcg tgatgatgct ttcaatcagc
    33061 tggcaattga cgttagtcgc gttattgatg atccctttaa gcttgatcat cgtgatgatc
    33121 gtagcgccga aatcacaagt gtttttcgca gatcagcaaa aaagtttagg tttattgaat
    33181 aaccaagtag aagaaacata tggtggtcat accattgtta aaacattcaa tcatactgaa
    33241 aaagaccagg aagtatttga aaaagaaaat cagaatctgt atgccgctgg ttggaaagct
    33301 caatttattt cggcaatcat catgccgttg atgaatttcg ttaaaaatct aggctatgtg
    33361 ttcgtcgctg tactaggcgg agtaaaagtt gcaaatggga acatgacatt aggggatgtt
    33421 caagcgttct tgcaatatac aacacagttc tctcaaccaa tcactcaact tgcaagtttg
    33481 atgaacacta tccaatcaac agttgcttca gccgaacggg tatttgaagt gttggatgaa
    33541 gaagagatgt ccaatgaaaa atctggcctt ccagaagaaa aagatacacc atacaaagtt
    33601 cgttttgaaa atgttcaatt cggctacaca ccagataact tgctaatgac tgattttaac
    33661 ttggatgtca aacctggtga gatggtcgct attgtaggtc cgactggtgc agggaagacg
    33721 acactgatca accttcttga acggttctac gatgtttctg gaggtagtat tcgttacgat
    33781 ggtgtagata cccgtgatct ttcacgtgat gaattgcgcg cacagttttc aatggtactg
    33841 caagatactt ggctgtttac aggaagtatc tatgacaata tcaaatatgg gaacgacgat
    33901 gcaacagacg aacaaatcat tgaggctgct aaagctgctc acgtagacga ttttgttcgt
    33961 aaattgccag aaggatataa cactgtatta aacgaggatg caagcaatat ttcgcaaggg
    34021 cagcgtcagc taatcacaat tgcgcgggca ttcttagcaa atccagatgt cctgatcctt
    34081 gatgaagcaa catctagtgt agatacacga acagaaatac tgattcaaaa agcgatgaac
    34141 cgcctattgg aaaaccggac aagtttcgtt gttgctcacc gcttgtctac aatacgcgat
    34201 gcagacaaca ttattgtgat gaatcatggt tcgatcgtag aaacaggaaa ccacgataca
    34261 ttgatggaaa aagacggatt ctatgcagat ctttataaca gtcagttttc tgaagaagaa
    34321 gcttcataga atagtacaaa tttattattt tttttagggg ctgtgacaaa agtcgtgtca
    34381 cagtcccttt ttccgaataa acggtgttca atcagctgtc cttcggtatt agctcaaaaa
    34441 acggcaaaat atgagaagct atttttccgt tttgtatctc taatactcag gtcaaaccgc
    34501 tgattttaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagccc
    34561 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcaga
    34621 acagcttttt cacaacctct ttttataaga gtttttactc attaatttac aaaaaacaag
    34681 caaaatgcga attttctcac atttttttta acaaaatcaa agttattgct tgaaagaaga
    34741 agaatccatc gttaaaataa agcttgacgt agaggggagc aagaaatatg aaagattttc
    34801 aattgggggc tgccacctat ttaaaaagtg tagccattcg ggtgaaagac cgagataaaa
    34861 tgattgattt ttacaagaac gttgttggct ttgatttgaa aagagaagaa aacgaattag
    34921 ccattttagg aacagaagaa gctggaagtg aaatgttgct gctggaagaa agtcctagag
    34981 cgaatgatca catgggggtg atcaaaaagc tgaaccgttt ttcattgatt atccctagca
    35041 cagaagaaat ggcagatatc ctttgtcgta tccgtaaaat gaattatcca atcaaagaag
    35101 ctttagaaga tcatggacgt atggggattt tgctcgtaga cccagaagag aatgaattgg
    35161 aaatttacca tgaagagaat aagaacgacc aaagagaaat tccagaaaca atggatcaag
    35221 aagcattatt aagaaaatct gaagaaaaat tcagtaaact atcaacaggt tcttattttg
    35281 aaaaaatcca tttaaatgtt actaacttaa caaaagaacg ccaatttctt aatcatgtac
    35341 ttggtttgaa ggtacataat gaagaagagg gcatccatgt attgaatgaa ggagatttcc
    35401 atgttggttt gacagaagct agcggaggcg caattgattt gcctacagat gaagtgattg
    35461 gacttgattt tctgaaattc atgatcgata ctgaaagcat ggaaggactg attgctcact
    35521 tgcagcaact atcagttgat ttcttcttag acaaaaagca cacagtactc acaatctatg
    35581 atgctatagg aatcgaatgg tggttcgtca gtgagccaaa agaaaagaat aaaactgaca
    35641 ttcgatgcga cgcttaactt tttctttctt aacgattgtc gcatgtctcg tcattttatg
    35701 aacggtcata ctatcgctta ttctagagat agaaagagaa aatagtatac cgggggaaac
    35761 tgcgcggttt ttcgcgcagt ttttttgtgt aaaaaaggtt gattcctcat agattaagct
    35821 ttaagcttgg actgtaagaa tctgatagaa aagcaggaga aaaagatgta tccacacacg
    35881 aaacagctta ttttgaaaaa gatggaagaa ggattttatc caggtgtagc agtcgccttc
    35941 ataaaagaaa atcaagtaga aacatttaca gccggctttg cccaagtcgt tcctgaaaag
    36001 cagctgatga cagaagatct gttgtttgat gcagcctcac tgacgaaagt cgttgcaacc
    36061 acttcattag ttttacgtct attagaagat ggaaagatag aactcgatca accgcttcat
    36121 aaatatcttc ctgaatttga aaatcagaca atcactttgc gtcatttact gacacatact
    36181 tctgatatca aaacatggat accaaacaga gataagctat ctcaggaaga attgatcgcc
    36241 gcttatttga agctagaacc aggtgagcaa ctaggaaaag aagtaaaata tacagatgcc
    36301 ggtacgattc ttcttgggtt gatgctagag gaaatttacc aagaaactgc agtggaaatc
    36361 tttcaagaac aagtattgga accattagga atgatgaaca gcttatttct tccttttcct
    36421 tccaaaaaaa tcgttcccac agaacaactg ccttcgggtg aggttttgcg tggacaaact
    36481 catgatccga aagcaagagt tttggctgaa catgcaggta acgcgggtct ctttattgat
    36541 ttgaaagaca gcattacttt tgtccaaatg tatttgaaca agggaaaaat ccgtaatggt
    36601 cgttttttag aagaagagac gattgattct ttactgcaag atcaaacacc gaaaaaaaat
    36661 ggctatcgtt ctttaggatg ggacttaaaa aaggatgtag aagacggtca tgcgattctt
    36721 tttcatactg gttatacagg tacgtttttc ttgattgata taaaaacaca agaagcgttg
    36781 atatttttgt caaatcgcgt acatcctgtc gatcatcgtg cggagtatct catatgtcga
    36841 gatgaaattg ttgaaaactt tttgaaagag aaagcggtat taccgaaata atgctataat
    36901 agctatgaaa aatgagagga gtttgaatta tgcaggaacc tatgttttta aaaccagttt
    36961 ttcaagaaaa aatttgggga ggaagtcgtt tacgttcagt ctttggtttt gatatcccta
    37021 acgataaaat cggagaagat tgggcgatca gtgcgcatcc tcatggtgtc agtgtagtag
    37081 aaaatggcga attcaaaggg aaaagattag atgaactatg gtcagaacac aaggaattgt
    37141 ttggccatcc ttcagaacca gtgtttccat tattgatcaa gatcttagac gcagaagatg
    37201 atctatctgt acaagtacat ccagatgatg cctatgggat gaaacatgaa ggtgaattag
    37261 gaaaaactga atgctggtat atcatcgatg ctgaacctgg tgcagaaatc atctatggtc
    37321 atcatgcaaa gacaagagaa gaactagctg agatgatcaa agatgggcgc tgggatgacc
    37381 ttttgaagaa agtaccagtt aaaaaaggtg atttctttta tgtaccaagc ggaacgatcc
    37441 atgctattgg taaaggaatc atgattttag aaacacagca aagttctgac acgacttacc
    37501 gcgtgtatga ctacgacaga aaagatgcgc aaggacaaac aagagagctg catatccaac
    37561 aatcgatcga tgtaacaact gtccctgcga aaacaccaga attacaaatc aaagaagtcc
    37621 gaaaagggaa ttcatcgatc gtcacttatt tagaaactga gtttttcaat gtatatgagt
    37681 gggatattaa aggaatcaca tctttcaaaa aacaagcccc atatacattg atgacggtaa
    37741 tcgaaggagc aggagacctt gtcgttgacg gaaaaacata tccattagaa aaaggaacaa
    37801 gctgtattat tccaaatggc gtttctgagt gggcggtcca aggtgaacta gcaatcattg
    37861 cgtctgaacc aggcaaatag ttcccagata gctgaaagca gcagacggtt tttcgtttgc
    37921 tgctttttct acataaaatc cgtccatcaa taaatagaag tctggtgaaa aagatggaat
    37981 acaaatggaa agcaaacaac agcagtcgcg tgtctatgaa acaactgaga aaagctggac
    38041 atcacttaaa tgaaatgatc atgccaaatc taagtctgtt tatcgcttgg ggaatcttgt
    38101 cattagcaac aaaaggtgtg tctggcgatt tgcacaccac attagaagag gtatgccatt
    38161 ggatgatcca gctgctttta cccatcttga tcagttattc aggcgggaaa tcggtagggg
    38221 gacaaaaagg ggcagtcgcc ggtgcaattg cttctttagg gttgatcgct cgttccaatg
    38281 ctccacaaat cgtaggagcg atgattatcg ggccgcttgc cggcatcgta tatgaacaat
    38341 ttgtttctag attccatgtg aaattcaagg ctggatatga aatgcttgtc agcaacctac
    38401 tagtaggact gactggtagc gttatttgta taattggaat cgtttttatt gaaccagttt
    38461 taaatagtgt tcacttgttg ctcgcctccg ttgtgtcttg gctagtcaca atgaatctgt
    38521 taccacttgt cagtctgatc ttagaacctc taaaagtact atttttcaat aatgcaatca
    38581 atcatggtgt attgacacct ttaggaatcg aattctcaca aacagtcggg tactcttcct
    38641 tatttttgat ggaggcaaat cctggacctg gtttagggat cttactttct atcctttgtt
    38701 ttgcagaaaa acaggaaaaa gtaaatgcga gtggagcatt gatgatccat gcgattggcg
    38761 ggattcacga aatctatttt ccatttgtct tgctgcgacc gtatctattt ttagctgtaa
    38821 tggctggtgg ggcaagcggc acgttgatat ttcaatggtt agatacggga ttagctgcac
    38881 ctgtatcgcc tgggtcacta ctcgtgattt ttgccaatgc gccttatcca caactagctg
    38941 gggtaagtat agggatagca atcagtacat ttgtgacctt catttgtgca gcgatactat
    39001 tgaaattacc aaataaagaa aaagagaaca aaacggtcaa agaaagggga gaaatcttgg
    39061 aaccatctat cattcattcc gtcatttttg cttgcgactc aggtatggga tcaagtgcta
    39121 tgggagccgc cctgctgaaa aagaaattag cagcagaaaa aatcgatctt tctgtcggat
    39181 acagctctat ctatgaattg aataaccaag taaatcagct ggttatcgtg caacatgaac
    39241 tagcgggtct tgcaagagaa aaagtacctc aaagtcaaat ctatgtactg gatcactttt
    39301 tggaaacaga tatcttgctg tcacgaataa aagaagcgat ccaatttcca aagcgggaag
    39361 aaaaaaaaga gatagcagat atcacagaac aaaaaacgtc gcctgctaat attgtatttt
    39421 tgtataaaaa caacagacgc ggctcacaaa ctatgggcat gacgatttta agtcaattag
    39481 caaaaaaagc gaaaaaacag tttgttatct caaaagaatc aatagaaaaa atcaaaatcc
    39541 aagaagaaac gatttatatc gcacaaagag aactagctgt aactgaacag ctagaagaaa
    39601 agatccctcg gctagttcta gttgaacatt gggtagctac gaatgaatac gaaaaactcg
    39661 tgaagggaga aatataagat gtatttatcg cccagagaaa aacaattatt agcagaattt
    39721 ctcagtagcc cgtcacctgt atctatccaa aaaatgatga atttattgaa ggtcagtaaa
    39781 cggactattt accgcgaatt agataatctt gaagtctcat tgaaatcggt aaatgcttct
    39841 ttgaaaaaag cagctagagg ctcatttgtc attgaggcat cagaagaagt attagagcaa
    39901 ttaaaagaaa atacagatgg gaaaagtaat gacttatcca ctgctaaacg acaacacgcg
    39961 attttagccg agttgttact gacgacagag ccgttgagtc ttgctcattt catggaatca
    40021 tatcgcatca gtaataccac tttttatgca gatatcaaac agttggaaga aagtattcgc
    40081 caactgcctt tggaaatcat tcggaaccag ggatacgaga tcgaaggacc ggaaaaatac
    40141 cgccggcttc tgacagcgaa tgtattagaa ttagaaatca atgaatatga actttttcat
    40201 agtatttctt tcgatgcatc actgaattat ttttttcgat tcgttgatcc gcaacatcta
    40261 tctcttgcac gcaaagtaat cggggaagaa ttgatccaaa aaaagaccaa tctaagtgat
    40321 agaaagcttg aacatttggt tttgatgctg acattgacga tggagcgtgt gacgaaaaat
    40381 catttactga caaatgagac atataatagt ctggcaaaca aagaattact acagacagct
    40441 aaacgattgt tttcacggat cgcttctgag acaaaacaac tttatccagt aaatgagatc
    40501 gtcttttttg caggattatt aaatgacttc acgaactcat ttgatcagaa tttctttgaa
    40561 gagacgttcg atacacagct tgcttattta gtaaaacaac tgattcaaga agtaagtgaa
    40621 gagacgaatg tccattttta tgaagatgaa actttgtaca aaatgctgtt gactcattta
    40681 tctggtgtgt tttcaagagc cgttttacaa gaagagacac tgaccaatcc gattttagaa
    40741 aaaatcatgg ttcaatacga ggaagttgcg aatgcaatcc gtaaagcagt agagcggatt
    40801 tttcaagaaa aaaaattatc cgaagaagag atcgcttata tggtccttca ttttgcgaat
    40861 tcattggaac gaagtccgaa agaaagcaat atcagtattg caggattttc gccaagtgga
    40921 ttagcttcta ctagcatgtt ggaactacga ttgaaaaggt attttccctt catccaacaa
    40981 attcatttct atcggattgc tgatttaaaa aaaatcgatt tacaagaaga atatgatgtt
    41041 gttatttcta catctatttt accaggatac ggtggaaaat atattttagt ctccccgcta
    41101 ctgctagaag atgaagtcaa gcagctaaaa gaagcttttt cgcatattga gaagaaaaaa
    41161 aggcaaaaaa ttacgtataa cagcaataac gagtggatga tagaagatac ttatgaggaa
    41221 acaatgcatt tcatggatca aatcaatcaa ttactcggtc gtttttttat ccaagaactg
    41281 gataatcctg agacgatttc cgaaacgatt cgcagcttat ttccttattt tcctgaagga
    41341 ctagtaacgg atgaagaggt ggttcatcgc cggctgctta agcgatatga acaagcacct
    41401 atcggtattc ctcatacaaa tatggggtta ttccatacat ccagctcatt gatttctaaa
    41461 ccgatctttt gtattttcaa tttgaaacat ccgttgacca ttgaagggat ggatagacag
    41521 ccagtcgatt tgtcccgtat gttagttatg ctggcgcctt cgccaatcca agaaaaagac
    41581 gggattcttt taggaaagat cagtggagca atcatcatga atgacttgaa caccgagatc
    41641 ttccgctcag gaaataaaga aatcgtttat cagttgttag cgaaaatcct gattgaagaa
    41701 ataaagaaat gaaggtgaaa agatgataca gctaccagca gaaaaaatat tgctcgatca
    41761 atcctatcaa aattggaaag aagcgttgac cgagatcggc cagtggatga cccaacaagg
    41821 gggaatcaaa ccggcatata tccaatcaat gatcgaaaga caagaaaaag cgagtgtcta
    41881 tatcgggaat ttcgtagcct tgccacatgg gactgacaaa gatgaagaag tagtagaaga
    41941 aggaatctat ttagtccaag tgcctgatgg tgtggacttt ggaacaaaag aggaaccaaa
    42001 aatcgcgaca ctcttatttg tagtggcttt aaaaaaagaa catcagttag cggtgctcca
    42061 agatttagcc tttttctgtt cagacatcga ccaagtaatg gcgctcagtg atgcggaaac
    42121 cattgaagaa gtccaaatga tttttgaaca tggacatata taaccaatgt tcacagtaaa
    42181 aaaagctgcc ttgcatcaag taataacttc aaccaagata aaaataagag gagctatttt
    42241 tgttgggagg ttcttattac tgcggatgca agcttttgaa aactatttat tctagaaaac
    42301 cgtttatttg aaatagggga cggtgacaag gtttttgtca ccgcccctat tatttttgtg
    42361 ttttaatagc tcttttttat atagatagtg cccataccta tgaataataa aacgatactc
    42421 catataccaa gtgtcaagat accattcatg aaagtaacct gttctgaagc tagtggcgag
    42481 aaaatatctc ctcctatgat cacattttga aaatcaacat aagaagttgg caagtaatga
    42541 ctgattgatt ctatccatcc aatcgaagaa aacaagcgat actctgctaa gaaaagacaa
    42601 agaagtgtac ctaaaagatt gactagagaa tgttcaaata aagcagatag aagaaaactg
    42661 attgtactta aaaagagcac ccacaacaac aggaacagaa agctctggaa aagatactca
    42721 tacatcggca ggattcgtat ctcctgacca atgatcgttc ctacaggata acggaagtct
    42781 cccaaaccat ttttgattat tactagtata gagacgatca aaaatggcat cacgagactt
    42841 agcatagctg caccataata aatggtaaaa cggatgctat gtttctttac catactatcc
    42901 ggtaatagat tgactagaga gattacctgc tttctttttt cataagagat gatggaagaa
    42961 agaaacaagg cggttataca taaaatcaaa aaactagaaa tcattcctga aaaaatattt
    43021 tcgtagtaat ttgctaaaga gagatgcgct ggcaagtcaa aaatagaata tctttggatc
    43081 tgatgttctt tcatataacg gagcaactcg acacgttttt gttgttcaat gattggaatt
    43141 ccttgtcttt gaccagaaat cagtcgatcc agtatatctt tttcataatg ataagttgct
    43201 tcgaccattt gctggacatt ccctgtgttt tttgcatgta agattgcttc gatcagtttg
    43261 ttgcttttct ctaaactttc cacaaattct ttctcattct catcttcttt caacgtttca
    43321 atcaaacgtt gcatctcttc atagttatga ttcagttcat cgaattctgt ataagcaagt
    43381 ttatgatcaa aaaaaagtgg gaatgcaaag agcacgacca acaatagagg aaataaaaag
    43441 tttactttgt tttttagtaa gagattgcgt tcaaatgtaa gtaaagacat agttacacct
    43501 catataattt acgataaata gctaaaatct cttccatatt tgaggattct gtgacgattt
    43561 ctccattttt tagaaaacat gctttagaac aaatcgattg gatattcgcg atagcgtggg
    43621 aagaaagaag aatcgtcttc ccctctttct gccattgtct gatgaggcta ttcaagatat
    43681 caatacttcc tgggtctaat ccgttcaacg gttcatcgaa taacatgaca gatgaatcac
    43741 tgatcaagta agcagcaagc aagacatgtt gtttcattcc tagagacatt tttccaatcg
    43801 gcaaagtaag gtactcagtc atcttcaatc gttggaaaac agtttggcat tctactttgc
    43861 tctgccaata gtatttgatc aaagtcaaat aatcaacagc cgatagatag tcggataact
    43921 gattcgttga ttctagaaaa aagaaagacc ggcgataatc tgtataagca gtaggagaga
    43981 tgttgtgaag ggacaattga tcataagttg ttggcagcag ttgggcaatg ccattcagca
    44041 aagttgtttt gccaaatccg ttcggggcaa tcaaagcagc tacttctcct gattctacta
    44101 aaagattcaa ttgtcgaatc acacgttttt taccacgttt catgttcatg tttttgatca
    44161 gtaatgtcat agataggcct ccaatataaa atttaataac ataatagaat ggaaaacatc
    44221 taaattaaat gctaacaaaa gttattgttt gcatattata aaaaaattaa aataacagtt
    44281 ttatttaaaa ttgaagtttt ctgataccga aatcttttta ttttgctgac atactcaaac
    44341 ctttggcaca aagtttcgtg ccaaagtacg actatttctc tttttagtaa gcggatacaa
    44401 ttagagtatc aaaaggagag agggagagac aaaatggaac ggacgattcc caaaaacaac
    44461 atttcgctaa aagcgagggt acaaaaatta ggcagtactt tatcaagcat ggtcatgcca
    44521 aatattggag cactgattgc ctggggtgta ttaacggctc tatttatccc agatggttat
    44581 ttaccaaatg aggctttcgc taccatggta agcccaatgc taacttatct gattccttta
    44641 ttgatcggtt atactggcgg aaaagtaatt gctggtgacc gcggatcagt tgttggggca
    44701 attgcaacga tgggtgtgat tgtcggaaca gatattccaa tgatgctagg cgcgatgatc
    44761 atgggacctc ttggtggata tgccatcaag aaatttgatc agctattcca aaaacggatc
    44821 aaatccggtt ttgaaatgct agtcaataat ttttcagcag gtcttatcgg ttttggatta
    44881 gcgttattag gtttttctgc gattggtcca gttgtcgatg cattgactca agcaatggca
    44941 aaaggtgtag agatcatttt atctgcccat ttgattccat tgacaagtat ttttattgaa
    45001 ccagcaaaaa tcttattttt gaataatgca atcaatcatg ggattttgac accattggga
    45061 actgaacaag tagcagaggc tggaaaatct attctattct tgcttgaagc aaatcctggt
    45121 ccaggacttg gcgtattact tgcttttact ttgtttggca aaggagcagc aaaatcttca
    45181 gcaccaggag ccatgatcat tcacttttta ggcgggatcc atgaaatcta ttttccttac
    45241 gttatgatga aaccgttatt attcttatca gttataggcg gaggaatgtc aggaagtttc
    45301 attttccaat tgatggatgc tggactaaga gcgccagctt ctccagggtc gatcattgcg
    45361 attctagcga tgacaccgtt caattcttac cttcctgtta ttttgggcgt gattgttgcc
    45421 gcagctgttt cgtttggtat ttctgcagta attttgaaag cggatgcgaa agatacaaat
    45481 gaagacttcg aaaaatctgt tcaagagaca aaggcagcta agcaagcttc caaaggtgcc
    45541 caaggtattc ctcaaggagc tacattctct ggcgtacaaa aaatcatttt tgcttgtgat
    45601 gcaggaatgg gatcaagtgc gatgggcgct tcgatcttga gaaaaaaagt acaggaagca
    45661 gacttgcctc aaacggtgac caatcaagca atcagccaac tgacagatga ctcgaataca
    45721 ttgatcgtta ctcaggtcga actacaagaa agagcaaaac aaaaagcacc gagcgcgcgt
    45781 tttgtctcag tagaaaactt tttgaattca cctcgatatg atgaaatcat tcaagaatta
    45841 agcagcaagc agaaaacatc agtttctgaa attgagaatg agccagcaga agagtttctt
    45901 tcagcaaaca tcaaacaaat caaagagatt ctacttgtcc acgatgatcg ttccggatca
    45961 gcaacaatgg ggatgaccgt gctcaaagat atattagaca agaaccattt aactatcccg
    46021 ttaagaaaag tcaatatcca agatttaaaa gatgaacctg attcactgat cgtagccaaa
    46081 gaaagtttag cggaaaaagt acagatggct gcacctcaat ccattcattt atcagtcaac
    46141 agcatggtca atccgcaaaa atacgaatcg attgcacaat atttgaaaca gacagcttaa
    46201 gggaggaaaa cagcatgcaa agtttgccat tcgacatgat cgagctcaat cgatctttta
    46261 aaaccaaaga agaagcgatc cgctattgcg gtcagatgct tgtagaagca gggtgcgttg
    46321 atgaggaata tatcgatgcc atggtagata gagacaaaat gttatctgtt tatatgggga
    46381 attttatcgc gattcctcat ggaacagatg aagcaaaaaa atatgtgaaa aaatccggca
    46441 tctgtgtcgt tcaagtacca gatggtgtag attttggtac cgaagaagaa gaaaagattg
    46501 cgactgtttt gttcggtatt gcaggagtcg gagatgagca tttgcaactt gtacagcaaa
    46561 tcgcacttta ttgcagtgat atggacaatg ttgtccagct agcagacgcg ctcagcaaag
    46621 aagaagtaac ggaaaatcta gcaattgctt aaggagagag aaaaatgaga gcagtacatt
    46681 ttggtgcagg aaatatcggt cgaggattta ttggagaggt tttggcagct aatggatttg
    46741 agattacttt cgtagatgtg aatcacacga tcattgatgc tttaaatgaa agaaaagaat
    46801 acgaaatcga attagcagat gaatcaaaag aaaaaatcac tgtaaaaaat gtcaaaggaa
    46861 tcaacaatca aacaaatcca gaaaaagtaa tccaagaaat cgctgaagca gatttagtta
    46921 ctaccgccat tggcccgaat atcttacctt ttattgctga gttgattgca aaaggtatcc
    46981 aagaaagaga aaaggaagga agcacaacat ctttagatat cattgcctgt gaaaatatga
    47041 ttggcggttc agcatttcta gaaaaagaag tgtataaata tcttccggaa acatcattta
    47101 ctgacaaata tatcggcttt ccaaatgcag cagtcgatcg aatcgtccca ttacaacacc
    47161 atgaagatcc tttattcgtc caagtggaac ctttcaaaga atgggtcatt gacgatagcc
    47221 aaagaaaaaa taaaaaaatc caattaaaag gggttttata tgtagatgat ctagaaccat
    47281 atatcgaacg caaactattt agtgtcaata ctggtcatgc gactgtcgct tatacaggtg
    47341 ccttgttagg ttaccagacc attgatgaag caatgcaaga tgcgttagtt gtggcacaat
    47401 taaaatcagt tttgcaggaa acaggaagtt tgctgattgc caaatggggg tttgatgcag
    47461 agcagcatca tgcgtatatt gaaaaaatca ttcatcgctt ccaaaacaaa aatatctctg
    47521 atgcgatcac acgtgtcgct cggacaccat tacgtaagtt aggttatcaa gaaagattta
    47581 cacgtccagt gagagaacta caagaacata acttgacttg cccacatttg acggcaacaa
    47641 tggggattat ttttaattac tatgatcctg aagatgaaca aagccgtcaa ttgcatgaaa
    47701 tgaagatcca tgaaaacttg gaacaattga ttcaagaagt cacaggaatc aatgatccga
    47761 aaacaatcgg gaatatcaag caaaacgtga accgttatgc caagcaagtc gcttaaaata
    47821 ggatacgaaa aaggggaatg tgacgaaatt tttgtcacat tccccttttt cgaataacta
    47881 ggctgtttgt ccttcaagat agggcagatc caattcttta ccgtaacgtt gttctaaagc
    47941 taggcggatc aaacgagctg ccaggttctc atttcttgca aggataggtc catggaaata
    48001 agaaccgaag acatttcggt aaatgacacc ttcagattgg tcttcaccat tatttccttg
    48061 accttgtacc actttcccca aaggtcgttc accttcacct aagaaagtcc gaccattgtg
    48121 gttttcaaat ccataataag tttcattgaa ttcttcgtta tggataacga tatcaccgat
    48181 aaaccggtta ttttcctgac ttaacgtgta gtgatccagt gcactgattc cttggatctt
    48241 ttctccatta gctcccatgt agtagtgacc tagcaattgg tagccaccac aaatggctaa
    48301 catgacgcca tcattttcga tatagttgat caaagaatct ttctttttct ggatgtcttt
    48361 tgaaatgatt aattgctcaa agtcttgacc accgccaaag aaaaccaagt catatttatc
    48421 agcatcaaaa ggttcatgta tactaacgat ctccgtatgg caagaaaccc ccattttttc
    48481 tgctatatat ttcaacatca aaaggtttcc gttatcacca tatgtgttca tcaaatttcc
    48541 ataaaggtgg gcgatattga gttcataatt agccacggtt cattcctcct ttgataaatc
    48601 cttgagcagc tagttcttta cgaagctgca atacagcagt gtaagtagct aaaatataga
    48661 catgctcagt tggcaattct ttgatttttt cgattacttc tggtaattct tttgtttctg
    48721 tcaatttatt ttctggaatc ccggcaactt ttagacgtaa agtcatatct gtatgacggt
    48781 ctcctccaga aataacagct gggatatcca tatcggcaaa gttttcaaaa ttaccatccc
    48841 aaatccagct gacatcgatt ccgtctgcgt aattggcatt taataaagcc actaaagaaa
    48901 atggataagg agctagacca atcatatcga ttacttgatt gatacctaca ggatttttga
    48961 caagtactaa tgtacatttt ttcccattga tattgatgac ttcttgtcgg ccgaatactt
    49021 tctcgtcata tcctaaacct tgtttgattt tatcaggatc gacattgaag tactctgcca
    49081 ctgcagtagc tgctaaagca ttgtagacat tatacatacc gccaacttcg attccgtatt
    49141 catgaccatc gatcacgaaa tctgctgaca cattgtccat ccgtaccata tcagttaatc
    49201 gataatccaa ttcaggacgt ttgaagtcac aatttggaca gtaatatttc cctaagtttg
    49261 cataagtgat cattttataa tgcaagatgt gatgacaatt tggacataag acaccgtctg
    49321 tattgtaatg agccatttgt tcacgatctt cttcatggtc aaagccataa tattttctag
    49381 gattgactgt ttcgatagaa ttgaagattg gtgaatcacc gttactgata atcgttgcat
    49441 ttggtgactt tgcggcaccg tctacaatca ttttataggt cgtatagatt tctccatacc
    49501 gatccatttg atcacggaaa atatttgtaa ataagaacag ttctggtttc atatattctg
    49561 tgactttgct caaacttgct tcatctattt ctaggacagc aaactttttc tgtccttttt
    49621 ttggttttgc ctgcaagaaa gtcgatacaa ttccctgtac catattcgca ccagtagggt
    49681 tggttagaac ttcatcgaat tgctggcgca aaatattgac tgttaaagcg gtagtcaatg
    49741 ttttaccgtt tgttccggtg acaacgacga cttggtagtc ttttgccaaa ctatctagta
    49801 tatgtggatc gactttcagc gctagtttac ctggtaaact tgatccgcct ttaaaaaaag
    49861 tttttaatgc ccattgcgac gtttttccaa ctgcaatcgc aaaatgactg cgtattccca
    49921 ttatgtaacc tccaaaaacg atttgctgac gaataaattc aactaatcat accatatgtt
    49981 gaaaaaaagg ttaaattgct tttcttattc ttaagaaaag gttacggctg aaagtgcatg
    50041 aaatcggaca aaatcgcatg atttaacaaa aaaacagctt gaaacaaaat gattgtttca
    50101 agctgctgtg attttattat tccctcttac ctatattccc attttaaccg ataataattt
    50161 tctcgcttgg atattcatag cctagatctc gtgtttcttt tggcacgaat aacatcaatg
    50221 tatatagcat tccgatccgc ccaataaaca tcagtgtagc aatcgtgatt ttgcccacaa
    50281 cagacaagtc tccggtaatc cctaaagata aaccagttgt tccaaacgca gaagttactt
    50341 cgatgatgat cgagatcagc gtttgttctt ctgtagcaga taaaaaaacg atacaaaaga
    50401 aacacatacc aagagaaagc ataaagacaa cgactgattt tcgcacatca tcttgatcga
    50461 ttctccgtcc aaaaatattg atgttatctt cacttttgag gaaggaatat agatacaaac
    50521 cgatgattgc tacagtagtt gttcgaatcc cgccgcctac agaactagga ctgcatccga
    50581 taaacatcag taaagaaaaa atgatcagtg ttgtgatttg aaaatcaccc aaatcgtgaa
    50641 tctgcagacc agcattacga gtcgtaatcg aataaaacat cgaattgatc catttgacac
    50701 tcatattgga atcttggaat aaatggtcct tttccaatag ataaatcaag acagttcccg
    50761 aaacaaacaa gataacaaaa gctaacacag ctaatttggt aaacaaggaa aaacgaaacg
    50821 gaagcttcgc attggaacgt ttgtataata gccattcccg gcactccatc aatacaggaa
    50881 aaccaattcc tccgacaaaa atcaaaaaca tgatcaggat caagaaaaaa tagtcatgtg
    50941 caaagggctt gatggaatcc ccagtgacgt caaacccaga gttcgtgaca gccgatatcg
    51001 cctgataaaa accgtaaaaa acggcatcac gccaacgatc aaaatagcct ctgtaataaa
    51061 aataaataga aaagaatgtc ccaaaaagta attgaaacca gattaaaata gcaaaagtga
    51121 tccgaatcag tcggacgata ccagacaaac gcggttgatt catatccgtc atgatcagct
    51181 gtcgctgttt cagtgtgatc cgtcgtttag aaaaaatgac aaaagcagta gaaatcatca
    51241 tgatccccaa accaccaact tgaaataata tttccagcag tataataccg ttatcattga
    51301 acacagaatt gatatcaaaa gtcgatagtc cagtcacact cactgtactg attgccatga
    51361 acagcatgtc cacaaaagaa acatggctat ctggttcgcg aaaaatcggc atataaaata
    51421 gaacgagtga taagaccgtc atcaatatat aataaaaaac gatgatttgg atcgatgaga
    51481 aatgattggc agcaaacttc tgtcctcgcc gctgcagcct tcgaaagaat aaatcaacat
    51541 gcacagtact atcctcctga caatagtcgt ttctaccagt atacaagggg aattaggaat
    51601 actcaaagaa attatcaaac agataacaat acagctttcg attaaaatag aaatggctta
    51661 taataaggaa aatcctagaa aaaagaagga ggaggaataa caaattgatt aatgctcaac
    51721 aaatcgtaga aaaaatgaaa aatcaaaaaa taaattatga tacagttctg aaaaaaatga
    51781 tcatgcaatg ggagaaagaa gaagtaaagc ctaagatatt aatacataca tgctgtgctc
    51841 cttgcagtac gcacacattg gaattcatgt ctcaatatgc agagatcacg ttattttttt
    51901 caaattcgaa tattcatcct aaaagtgagt atttgcgaag attacatgaa caaaaacgat
    51961 ttgtagacca atttaatgag aaaactgacc atcatgttgc gctaatcgtt gatgaatacc
    52021 gtccaaatga attcaccaaa atggttttaa caaaccatct tgaaaacgaa aaagaaggtg
    52081 gtgctcgctg cagtgcatgc ttcaatatgc gattagatct tgttgcgcaa aaagcgcaag
    52141 aactaggata cgattacttt ggaagtgccc tgaccatctc gccaaagaaa aatgcagctt
    52201 tgatcaatca gatcggaatg gatatccaaa agatttacgg gacacaatac ttgccaagtg
    52261 atttcaagaa aaacaaaggc tatgaacgct ctttgcaaat gtgcaaagag tatgatattt
    52321 atcgccaatg ttattgtggt tgcgtgttcg ctgccaagca gcaaggatgc gatctgaaag
    52381 aaatcaatcg ggaagcaaaa gcttacttaa aaacatcaac cgtagttttt gaggaaaatt
    52441 aaagaaatct ttcggaaaaa cgtaagaaaa aaataaagaa tttatggtag aaataaggta
    52501 gactgtataa tttgtccgta gtaaagtaag gaaacaagaa taaatttcaa tgaaggaaga
    52561 gataagatgc gaaaagtagc acctattttg aagccaatcg ctgtattaac aatctcgacg
    52621 attgtcgcga ctactacagg tattgtgaat agttccagct accgtgcacc aagttcaagt
    52681 tctagtactg tagaagaaac aacaccaact acttcagtct cgacatctga accaacaaca
    52741 acggaaagtt tgaatgaaac cactacgaca agtacatggg acgatacaac aactagttcg
    52801 tcgtatgatg agacatatac aactgacagc tcaagcgaaa caagtaatac ggtaccagat
    52861 ggcactccat cggattcaac caatccaagt acaagtccgg atacaagtgc tagctccgaa
    52921 acgataccaa gcagtacgac tgtagatgat aacacgcaaa atgatgatgc agcacgtaca
    52981 gcacagcaaa accaagattc aggaaatacc caaacgtata accagcaagc aggtgattct
    53041 caatgacatt tttactagac cttatcaacg gggtgcataa attccttggc tatttagaca
    53101 tcagtcctaa atatcttaac cgtggatata cgattttaag tgtgattccc acgctttatt
    53161 tattgagaat tgtttatgga ctttggcaaa accaaaacta tcttcaattt ttcttatatg
    53221 gacttgcatt tctagtgctt gtttatttta ctgttttaaa tgtattttat tactttttag
    53281 ataaaaacac gaaagccgat gtcactcagc tttttgtgaa gtatttacct gatgaagcgt
    53341 tcaacatcca gacagaagaa tcgatagata atggaaattc gatcgataaa gtgaatacac
    53401 aagaggtcat ggtttcttat gacgaagatt atcaattgaa attagccgag aatatgcggt
    53461 atttgattgg aaatggtgag atcaaaacaa atgaactcgg tcgcatggat ggatttctgg
    53521 tcgatagaaa tacattgtat ccttattact atgtcaagaa aacaggagag aaagagtatg
    53581 ttctaaatat tggacgaagc tactcggaca tggaaaaagt agggaccatt cgacttgact
    53641 caacagaaga cacattggaa cctgtcggat tgttcatcgt cggcggtgat tttgtcaaag
    53701 acgggttacg ctatcatgag ccttatcgtc tgaaattgat tgcaaaaaag gcacagtcgc
    53761 aggaagaacc agtggtttat agtcgtagcc aaagaagaag acattcataa tgtgatgaaa
    53821 cgccgtgtat cagtggttgc gataaaaacg tttattcgga ataaggggga tgtgacacgt
    53881 cttttgtcac gttccttttt tatataacga gaaagataag acgaacacat cacttattct
    53941 ttcctttcat ttataaacat tgtagaataa agaaagtaca acgaagtatt gtgaaaaaac
    54001 gtaataaata acgaggaaaa tacaagtagg aggtaagctg tgaaagaatt tttgaaaaat
    54061 tgggggattt tgatccttgt attagcggct attttattag ctagagtgta cgtgtttaca
    54121 ccagttactg taaacgggca ttcgatggac cctacattaa gtgatggaca acgattgatt
    54181 tcatctaaga tctcaaacta tgaacgaatg gatattatta ctacaaagga accgggggat
    54241 gaagaacgga tgattgttaa acggatcatc ggtatgcctg gagatacagt caagatggaa
    54301 aatgaccagt tgaccattaa tggcaaaaag tatgatgaac cttatctgga tgagttcaaa
    54361 aaagaatttt cagaagataa gttacaagga gaatatgctt atagctcagg attccaagca
    54421 caagcagaaa gttcatccac ctttacgagt gactttgaat atactgtgcc aaaaggaaag
    54481 tatcttgttc taggcgataa ccgcttgatt tcaaaagaca gccgaatgtt tgggttagtc
    54541 gacaaagata tgatccaagg aaaagttgtt ttccgttatt ggcctctttc agaaattaaa
    54601 atcatgtgat gaaaaaataa aaagaagctg tgacatgttt tttgtcacag cttctttttc
    54661 cgaaaattcc ttcttatttc ttggagttgc tcactttttt tgcaatctca ttttaagctt
    54721 tttggctgat ggataagaaa agcgaagcgt ttttgttcat aaatagcttt tccccacgat
    54781 ggaaggtatg actagtagca gagacaaagc ccgttttttc caatcgatta ttcaattctt
    54841 cttggctaaa gccattatgg actttcggat gactaatttg ttcattcttg tcaaagtcta
    54901 cgatgatcaa ttgaccgcca ggagctaaaa tttcataaag ttttgtaaga atattctccg
    54961 tatcaggaat gtgtaaaagt acaagtgata gaaggatgag attagcttgg acagcaggaa
    55021 attcgacaga agcatcagcg tggatcgttc ggacattttt cagatctgct gcttggatct
    55081 tttcttctgc cattttcagc atagtttctg aagcatcggc aatgatcagt tctttgaatc
    55141 tttccgcaag cggtaaacta acaagcccag ttccgcctcc gtaatccagc aggacttttt
    55201 ggtggctgtc gcgcggtaga taagtagtta gttcttcgcg aataatcttg gctaatgctt
    55261 gacggtcagg ggaatcataa tggttagcga tggaatcaaa aatatttttt gtcatcaaat
    55321 agctccttac gtaaaacttt gaaattttaa cttattatag cacgtgaaaa gaaagaaagc
    55381 atgctatact caattcaaaa agggagaatc tagaaagaat atgccgatat cgtttgtaaa
    55441 agatagagag gaaaaaggaa agtgtgtccg agaaatatta ctagacttgc ctgagtggtt
    55501 tgggcttcct gaatcaacgg aaaaatatat cgaggaatca agtaaactac ctttatggtg
    55561 tgaaaaaaga aaagaagaat atttaggctt tatcactctt tcacagacaa gtgaagatac
    55621 tgccgaaatc tattctattg tatgggaata aaaaaagact atcatcacca aggattagga
    55681 acaaaacttt ttaaagaagc agaaggttac gcagcaaaac attacaaata tttgcaagtg
    55741 aagactgtag acgaggggca ctactctata tatgatcaaa ctatttgttt ctacgaatca
    55801 ttaggatttt caaggttgga agtttttcca aatttatggg atgaatggaa cccatgttta
    55861 gttcttgtca aaaaattgga acaaaagtga gcaatagatt taatactaat ctgtgcttga
    55921 tgaaaattct cactgtaaaa aatgctgaaa atcaagtata ataccatagg aacaaactgg
    55981 aaggtgaaaa agagcatggc acaactattt tttaagtatg gcgcaatgaa tagcggcaag
    56041 acgatcgaga tcctaaaagt cgcacataat tatgaagaac aaaataagcc agttgtctta
    56101 atgacaagcg gtattgatac aagagatggt ttaggcgttg tttcaagccg aatcggactg
    56161 aaacgtaatg cgatgccgat atttgaagaa acagacgtat ttgacgtaat tcagaaaatg
    56221 gaaagcaaac cctattgtgt gctgatcgat gaagcgcaat tcttaaaaaa gaaacatgtc
    56281 attgctttta cgagaattgt ggacgaattg gatattccgg ttatggcatt tggtttgaaa
    56341 aatgattttc gaaacgagtt atttgaagga tcaaaatatt tattgcttta tgcagacaaa
    56401 atcgaagaaa tgaaaacaat ctgttggttc tgccacaaaa aagcaatcat gaatttacat
    56461 tatattgatg gaaaccctgt ttatgaaggc gatcaagtgc aaattggcgg aaatgaagct
    56521 tattaccccg tttgtcgaaa acattacttt cacccatcaa tgattacaga aggagaataa
    56581 gcatgtacga tcaattacaa gcaattgaag accgttatga agagcttgga gaacttttaa
    56641 gtgacccaga agtgatttct gatacaaagc gttttatgca gctttcaaaa gaagaagcaa
    56701 acacgcgcga aacagtcgaa gtttatcgtc gttataaaga agtagtacaa ggaatcaccg
    56761 atacagaaga actgcttggt gaaaaactgg atgatgaaat ggctgaacta gcaaaagaag
    56821 aattatctga attaaaaaaa gaaaaagaag tcttagaaga aaaaatcaag atcttgcttt
    56881 taccaaaaga tccaaatgac gataaaaata tcatcatgga aatccgtgga gcagcaggcg
    56941 gagacgaagc agcattattc gctggcgatc ttttcaatat gtaccaaaag tatgctgaag
    57001 cacaaggctg gaaaacagaa gtgatggaag ctaacgttac cggaatcggt ggatataaag
    57061 aagtgatcat gatgatcacc ggagacaacg tatattcgaa attaaaatat gaaagtggtg
    57121 cgcaccgcgt ccagcgtgtt ccttcaactg aatcgcaagg gcgtatccat acttccaccg
    57181 caacagttgt cgtgatgcca gaagctgaag aagtagagat cgatattgaa gacaaagata
    57241 tccgtacaga tatctatcat gcttcaggtg ctggcggaca gcacgtcaat aaaacagcat
    57301 ctgctgtacg tttgactcac ttaccaacag ggatcgttgt tgctatgcaa gatgaacgtt
    57361 ctcaaatcaa gaaccgtgaa aaagcaatga aaatcttgcg tgctcgtgtg tacgataaga
    57421 tccagcaaga agcacaaagc gagtatgatg ccaatcgaaa atcagcagtc ggaactgggg
    57481 atcgttcaga acggatccgg acgtataatt tcccgcaaaa tcgtgtgact gatcatcgta
    57541 tcggattaac tatccaaaaa ttagatcaga ttttagcagg gaaattagat gagatcgttg
    57601 atgcattgat catgtatgat caaacatcaa aactggaagc tatgcaaaat gggtaaaatg
    57661 acctatcgag aagtcctgtc acgggcttct tcttttttag aggatcatgg gaaagaagca
    57721 tatagtatcc aatttttatt tttggaacga aaacaatgga aaaaacttga ttggctcctt
    57781 catatgaacg aagagatttc agaagaagaa cagcggttga tcgaaaccga tctgcgactt
    57841 ctgcttgcag atcatccacc tcagtatttg ctaggatatg cagattttta tgatcatcgg
    57901 ttaaaggtaa ctgaagcaac actgatccct cgaccagaaa cagaggagtt agtcgaatgg
    57961 tgtttagacg agacgcctgg tgtaccgtta gaagtgatag atattgggac agggactgga
    58021 gcaattgcta ttagtttgaa agcagcaaga aaaaattggc atatatctgc tgtggatctt
    58081 tcagaagaag cattagaagt agcgaaagaa aatgctcaga aagaaggaac gaaaatcagt
    58141 ttttatcatg gagatacatt aggaccagta atggatcagc aatttgatgt gattatttct
    58201 aatccgccgt acatcagtcg gaatgaatgg gaactgatgg atgaaagtgt ccggtcattc
    58261 gaacctaaaa tggccctttt tgctgagaac gatggattag ctatttatga aaaaatagca
    58321 gttgaagcac caagcgtact aaaatcaaat ggaaagattt ttttagaaat cggatttcgc
    58381 caaggtgaag cagtaaaaaa aatatttcag caagcatttc cagataaaaa agtggcgata
    58441 aaaaaggatc tttttggcaa tgaacgaatg atccaagttt atggttgaag ttgtggatga
    58501 aaaatttttt atttgttttt ttattataaa aaaacactta tttgacaagg atgtaaacga
    58561 ctttttggtt tatcaacaag tttatccact attgacaaat agattatcca caaacagtgg
    58621 aaaacttgag gataaactca gacaaaataa gaatgtatga aaaaggggtt gtggataaac
    58681 tgtggaaacg aaacgatatg atgaaacaga attgcaagag gcagccgaag cactgaaaga
    58741 aggagaactt gttgcttttc ctaccgaaac agtatacgga ttgggcgcca acgctctttt
    58801 accaaacgct gtcaagaaag tgttttctgt aaaagggaga ccgcaagata atccattaat
    58861 cgtccatgtc gcctcttttg aacaagtaaa agagtatgtg gacaactttc atccggcagc
    58921 agagaagatt gtgaagaact tctggcctgg accattaact ttgatattta agatcaaaaa
    58981 agacactttg ccctcagttg tgacgggagg attatccaca gctgcatttc gtataccgga
    59041 taacaaaaaa acactagaag taatcgaact ttccggtgtt ccgcttgtcg gtccaagtgc
    59101 aaatacgtcg ggaaaaccaa gtccaacgac tgcagatcat gtatatcatg atctacaagg
    59161 aaaaattacc gggattattg atgatggagc tacgcgaatt ggtgtagaat ccactgttct
    59221 tgatttaagt gatccaacag ccatgcctat gattttacga ccaggtgcag taacgaaaga
    59281 acaaatcgaa gcagtcatcg aatctcctgt tgctattgat cagcatcttg taaaagaaaa
    59341 tgaaacacca aaagcccctg ggatgaaata caaacactac tcacccgata cacgtgttct
    59401 gatggtcaga gatggcgatt ggtcaactgc agttcaatgg gcaaaaaata agaagatccg
    59461 tgcaggtgtc atagcaagcc ccgaaattgc ggatcaagtg cggacagata ctgcagctgt
    59521 ttatatgtat aacgacaact cagtagaagc ggcggctaaa ggattgtttg caggtctaag
    59581 aggattagat gaaccaacac ttggattaga tttgattttt gtacaagtgt atccagaaac
    59641 tggattgggg aatgcttaca tgaatcgctt gaaaaaagca gcaggacaaa attattttga
    59701 aaaatgatca attggttatt gtcgcattct tttagtatga tacaatgcta tcaagtaaat
    59761 cttctatcag tgaacgaaaa actaagcgta actaatcgta atagaagaga tacgaatatt
    59821 tggcaatttg tttttgatgg gaaaaataaa attgtaggaa aataaaaagg agctgttgtt
    59881 tgtggtagat tacaaaacgt ttgaccctga tctttgggct gcgatcgcca aagaagaaga
    59941 aagacaagaa cataatttgg agctgattgc ctctgaaaac tttgtctccg aagcagtgat
    60001 ggctgcacaa ggaagtatct tgacaaataa atacgcagaa ggttatccag ggcatcgcta
    60061 ttatggtggg tgtgaatttg tcgatatcgt tgaaaattta gcgattgacc gtgcaaaaga
    60121 attattcgga gcgaaatttg cgaacgtcca gcctcactcc ggctctcaag ccaacacggc
    60181 tgcttatctc gctttggtag aaccagggga cacgatctta ggaatggatc tttccgcagg
    60241 cggacatcta acacatggat caccagtcaa cttcagtggc aaaacctatc actttgttgc
    60301 ttacggtgtt gatccgacaa cagaagttat tgattataat gtggtacgta ttttagctag
    60361 aaaacatcaa ccaaaattga tcgtagcagg tgccagtgct tacggacgaa cgatcgactt
    60421 tgcgaaattc agagaaatcg cagatgaagt aggcgcaaaa ctgatggtag atatggcaca
    60481 tatcgcagga ttagtagcag caggacttca tcctaatcca gttccttatg cagatatcac
    60541 aacgactacc actcataaaa cattgcgtgg ccctcgaggc ggcatgattt tgacaaacga
    60601 tgaagcatta gcaaaaaaaa taaatagtgc ggtatttcct ggtattcaag gcggccctct
    60661 agagcatgtg attgcgggta aagcagtagc attcaaagaa gcattagatc ctgcatttaa
    60721 agaatatagt gaacaaatca tcgccaatgc aaaagcaatg gtcaaagttt tcaatcaagc
    60781 aattggaacc agagtcattt caggggcgac agataatcat ctgatgttga tcgatgttcg
    60841 cgagttaggt ataaatggaa aagaagcaga gagcattttg gacagtgtga atatcactgt
    60901 aaataaaaac tcgattcctt ttgaaacact aagtccattc aaaacaagcg gtattcgaat
    60961 cggcacgcca gcaatcacta ctcgcgggtt caaagaagaa gatgctgtaa aagtggctga
    61021 actagttgtc aaagcattac aagcaaaaga tgataacgct caactagacg aagtcaaaac
    61081 aggtgtacgc gaattaactg aaaaatttcc gttgcataaa aaataaaaat ttaaattcca
    61141 ttagtaaaca gaaaagttca atgttgggct tttctgttta ttttttggga aaacggattc
    61201 tttgacaata tcggtgatgc tctctagtat atgaggtgta attttagtct tcacactaaa
    61261 tctcagggag gaaaagtcat gactgatcgt atctcgttag aaaaggaagc agtagcattc
    61321 agtgatgcaa atgcgcctca tccgcgtatc tatgaactag caccggaaga aggaagagtt
    61381 ttactagaag aagtacaaga atcacctgtg aacaaatatt ccgtcgatat tgaagacttg
    61441 atggtggata ctggcgaatg gggaaaactc aatattcgtc tcgtacgacc agaagggaat
    61501 atggataaat taccagttat tttttatatt catggagcag gctgggtttt tggcagtgct
    61561 caaacccatg ataaattgat cagagaactt gcggttcgta cgaattccgt cgttgtcttt
    61621 ccagaatata gtcgatcacc agaagcaaaa tatccaacag caattgaaca aaattatgct
    61681 gtgttgcaac agctgaataa attagccaag tcaaaaaatc tggatctcga gaatctaatc
    61741 gttgcaggag attcagtagg tggtaacatg gctacagtca tgacgatctt aacaaaagaa
    61801 cgaaagggcc tcccaatatc caagcaattg ctgtattatc cagtcacaaa cgctgatttt
    61861 gatacagatt cctatcatga attcggagaa aactattttt tgacaagaga aggaatgatg
    61921 tggttttggg accaatatac gacgaatgaa gaagagcgtg ctgaaatcac agcttcgcct
    61981 ttgagagcaa atataaatga actaaatgat cttccttcag caatgatttt aacgggagaa
    62041 gcggatgtat taagagatga aggtgaggct tatgcgcgca aacttcgtga tgcaggggtt
    62101 cctgttacac aagtacgctt ccaaggaatc atccatgatt ttgtcatggt aaatgctatg
    62161 gatcagacaa aggcaacgcg tgcagcaatg gcattatcta cttcttggat caagaaaaaa
    62221 gaataagctt tgcttgtccg caggaaaact tcttttcaaa aacggatttt cttaggaaaa
    62281 atggcgagtt tgactagtca atcacttgat tttgttttac aattaaaaag aaacgacaga
    62341 acaaaggaga gcttaaaaaa tgggcaaatt tcaagtgatc gatcatccat tgattcaaca
    62401 caaactaact attattcggg acaaaaactg cggaacaaag gtttttcgtg aagtagtcga
    62461 tgaaatcgct atgttgatgg catatgaagt atcacgggat atgccattgg aagacatcgt
    62521 gatcgaaaca ccgatcactg aaacaacaca aaaaacgttg tctggtaaaa aagtagcgat
    62581 tattccaatc ttgcgcgcag gtattggaat ggttgacggt atcttggaat tgattccagc
    62641 tgctaaagtg ggacatatcg gtctttatcg agatgaagaa acattggaac cacatgaata
    62701 tttcgtcaaa cttccagaag acattgatgc acgtcagttg tttgtagtag atccaatgtt
    62761 ggcaacaggc ggatcagcaa tcatggcaat cgatgcattg aaagaacgtg gcgcgtcgaa
    62821 cattaaattt gtttgtttgg tagcagcacc tgagggagta aaagctttgc aagaagcgca
    62881 tcctgatatt gacatctata cagcttctct agatgaaaaa ttgaatgaac atggttatat
    62941 cgttcctgga ctaggggatg cgggtgaccg tttgtttggt acaaaataag taaatacgaa
    63001 aaagggtgtg acacaacttt tgtcacatcc ttttttggtc atttttaacg tttgatgcag
    63061 aacagctttt tcacagcttt cgtttgcata taaaaaaacc atcccaaaaa tgcagagatg
    63121 gtcaaattca aatctttttt agttctttgt agttaacgat tactacaccc ttactttgcg
    63181 tcatcaagtt cgcattttct gcagtttcat caatctcaac aacagcggaa tttttcaatt
    63241 gctttgaaat cgttccagtt tgcgacttgc cgtgaattgt gaacattacg tgagtattga
    63301 tcttgaattt ttcaccaatt tccgatggta cgatagttgt atcaaatttt ccaaaagttg
    63361 ccattttcat aaaacctgcc tttcacatca attataccac gttttatgaa aaatatcatg
    63421 aaattaaact aattttccat cggactcttt acaatcagcc aaatagagta tataatttct
    63481 tttggataag gccagcttag ctcagttggt agagcaacgc actcgtaacg cgtaggtcgt
    63541 aggttcgatc cctatagctg gcattaaaag agaagacaga tatatcaagg atttttaatc
    63601 cattgataca tctgtctttt tgtttaaagg aaataaagta tgctttctta gcaatagagc
    63661 atgtattatt tctataaaaa tttaggagaa taaaacgact agcaatatag ctgtgaaatc
    63721 aaatatcaca tgaactgccc aacttgtaat gatcgagttg cttttaatgg cagcttggtt
    63781 gaataacaat cttgccaccc cttgtatcaa aaggatgtgt gctacagtgt gtaaaatatt
    63841 tccattatca taagtactgt aatggataaa tccaaagatg attgctgaca agaaactagc
    63901 gacaggaatg ctgaacttac tagaaaaaat agcgagaaaa tacactgaga acaattcttc
    63961 tccaagtatc atgataggca aaataataaa taataacggg ctgaaagcct catttattgg
    64021 attgcctgtt aaaccccatt tcaaaatttg ttgcaaaaat accgagactg cggcactgat
    64081 tactacattg ataaaaaaat atttggctat atttttgaca ggagcgacag gtttcgagaa
    64141 catttttttc gtatcttttt taccaaaaac aagtgtaaat gctataaatc cagtgatagt
    64201 taaagaaaaa atgctaataa tggatggcgc aaatagcaac cctaacaaac cgagtacaac
    64261 gacaatagtg cccaatatcc cttttttaag ttctattttt tttcctaaaa tgttattcat
    64321 gataataact cctactattc agtttttgaa gatagagtca gtatacatga agagttaaaa
    64381 taggcaatgt ttttttatgg atatttgtat tgctaaaaat ttatcttata tattcatttt
    64441 gattatacta cttttatttg taaaaatttt ctagcaaaac caagtgcttg caactgatta
    64501 agcgtacaat tttttagatg ttccgagcgt acttcaatca tttcaaagga gttgctactt
    64561 aaatcgatat tttttaaatc agtatcaaaa aagctagcat tttctaaatc attagtagaa
    64621 aacattattt tagtgaggga acagtctaca aaatctgcgc ttgataagac agagtgctca
    64681 aaggtacttt cttttatgtt tgaaccagat aaatttacat agtccatttt acaattctcg
    64741 aataacgtgt tcccaatata actttccatc aatttagtac ctgttaattt acaattgaca
    64801 aatttcacgc gatgcaacat gctttttgtc aatatacagc ctgaaaaatc acagttttcg
    64861 aatatacagt cggtaatctc cattctttga aaatcattgg aagcaaaaaa tgcatacttg
    64921 aaaatagatt tgtaaataat tggatggcag attatttggt tttcgatgac ctctttttgt
    64981 gaaaggatat tatacagttc aaaatcttcg ttttcataga tatgaaaaaa ggatccttta
    65041 ttcaagcttg cttcattaac tttaggaaaa atcatattta attacctcca ttttcataaa
    65101 gtcatattaa caaaagctaa accaacttac attagcagaa aatgagatag gttttcgcct
    65161 ttttcgataa cgatagtgtc gctatctctt gaaacgttct tagaattgcc ctgagagaca
    65221 caaattaggc gtaattctaa taaaaatctt agaataaaaa tttttactga tttaatttag
    65281 aatgttttta aaataaaaat cgttgccata acagtgtatt gaaaggtttt aacaatccag
    65341 taatgattta aaaaaatcta gatgtagcgt atactttgtt tatagaatgt agtgggaaga
    65401 aatatatttt tgggaggaaa tcgtgatgtc taaaagtgaa gaaatagaaa acatggtact
    65461 aaaattatat gatctcgttt taaatccaga gatcaaagaa aaggaacgta tacttttgat
    65521 agacgcaaag acaggcttag aaaaaggtca gtattatcct aaagtcataa ataatttgga
    65581 acgtagttta cgtccgttag caatcagggg agaattgtct aaaccagtat caccgtttta
    65641 tatggagata tcaacaatag gaaaatttga aaaagaatta ggtcgaggaa tggcttcggc
    65701 accgattact tttgggcatt tataaaaacg agttattcat ctcaatgaat taaaaagtat
    65761 agtcaatctc ataatgcagg ctaggttcat ttatttgaac agagtctgta tttttttatt
    65821 ctaaataaag aaacagatgt ttctttatat tgtattttat cctttaatta acacaaaaac
    65881 agctagtata aactaataat gttaattaaa agtgtatttt atgaaactat cgttatttat
    65941 taatgttgca tttttgtaag ttaaaaattg gaaatataga ttgaaaaaat gaaagcaata
    66001 agtaaaataa aatgaatata tgcgaaaaaa taatttacat taggaaaaac cttgtaaaaa
    66061 cagttttttg gcatatttat tttttataaa tatttgtaaa ataactatca attttttagt
    66121 taaaagattg gaaatgagtg ggggaggaat tgaagaaagg atttttgaga gatgatatag
    66181 taggttttat ttttagttaa aataggggag atagaaaaaa tgaaaaagtg tagaaatatt
    66241 attttatcaa tgttatttat tattactaat ataacatcac tgattcctgt tcatgtctat
    66301 gcggatgcag gcagagatat cagcagtaat gtcacttcgt tgaccgtgga cccaacgaat
    66361 attacagatg gaggaaacat aaaagtaaaa ttctcgtttg acgaaaaaaa acaaaatatc
    66421 caaccaggtg attatctttg gatcaattgg ccaagtgaag gaaatattcg cggtgaagga
    66481 ttccaaaaag aaatcccatt aatgattgaa aataaaaatg ttggaacttt aacggtcaga
    66541 aaagattctg cgcaggttgt gtttaatgaa aatattaaaa acctagactc agtagaaggt
    66601 tgggggcaat ttgagataca ggccagaaac gtaaccgata ccggtgaaga aaatacggga
    66661 ccattcaccg tgacgagcgg tgataaaaca gctactgtaa atgtgacaaa gccagcttct
    66721 ggttcttctt ctagcgtttt ttattataaa acaggagaca tgctacctga agatactaag
    66781 cacattcgat ggtttttgaa tattaacaat aacggcactt atgttgaaca accagttaaa
    66841 atcagtgatg aaatccaaag tggacaaaga ttagatccaa gtacgtttga aattaatcaa
    66901 atacacttag gtgaacagaa ggtttataga ggagaagagg gaatccaaca atttttacaa
    66961 gatttcccca gcgctacatt taatttttca gtaactgata attacattga aataactata
    67021 ccaaaaaact ttgtcaatct taggaaaata atggtgagtt ataagacgat catagaaaac
    67081 ccagaacaaa taaattttga gaatcattcg gaagcttggt ttaaagaatt taataaacct
    67141 gctgttgatg gagaaagttt caatcataca gttaaaaata tttcagcttc tggtggagtc
    67201 aacggtacag ttagaggcga attgaagatt ttcaaatata tcaatgacac agagattggt
    67261 atcccaaatg tgactttcga attaagacga gcagatgaac agcctattca aggacagtct
    67321 agtatcctgt tgacttcgaa tgaacaggga gaagctagta tcaaaggcct gcaagtagga
    67381 gactatgttg taaaagaaaa agaagcaccc aattggattg attttgatcc actaagttca
    67441 aatgaactca aattctccat aaacgaaaat gatacagagg gagtcagttt acctatttat
    67501 aacaagaaaa aggttacgaa catcacagca actaaaattt ggaacggtgg aaccactcca
    67561 agaccgagta tttattttaa attattcaga gcaagtacaa acaattggga accagttcct
    67621 gatgcagaaa caaaacgatt agacaatgga ataacttcag tgacttggga agatatccaa
    67681 cagtatgatg atagtggaaa cgaatatacg tttaaagtgc aagaagtaga tcaaaacgga
    67741 aatgattatg tcccttcagg ttatcaaaaa attgaaaacg ggctagtcgt tacaaatgag
    67801 aataaagaag tcatttcagt ctcaggacaa aaaacatggg aagacaatga gaaccaagac
    67861 ggcaaacgac cgacgacgat cacagtaaat ctattagccg acggtcaacc gatccaacac
    67921 aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca caaatctgcc aaaatacaga
    67981 gacgggcaag aaatcatcta tactgtaacg gaagacaacg tgcccgaata cagtacgacg
    68041 attgacgggt ataacatcaa aaacagttat acaccggaaa aaacaaatat atctatacca
    68101 ggtagccata taacaccgtg gaaaaatttt ccagagaaga cagagagaga gaaaattaga
    68161 aatctattct ctcaaaaatt gcaaatattt gctcatacag gagaatccag cgaacaaaga
    68221 agcaattata ttagtaagag agctgtacct aaacagataa caaataaatc aaaaagcatt
    68281 cttcctaaaa caagtagtaa aaaatctagt tttgctgtaa tcattggttt attgatagta
    68341 actatctgtg ctgggatatt cttacaaaaa tataaaaaag tttagttaga atataatatt
    68401 ttttgttaaa tatgactgaa atcaaaagaa taaaattccg atttataatc gaaaaaagaa
    68461 gcatttttat atatgcttct tttttcgtag aatcaataaa tatggatttg gctaactatg
    68521 gaagaatact cgtattagtt ttcagcgttc atactagttg ttacattgtt tgattggcca
    68581 aaagaatttc tgatatattt tgtgctaaat aaagaaaaag acgcttgaat tgggaaataa
    68641 gaaaactact ctatcattac aaattttcat aaactggcag atgttctaag taaacaagta
    68701 tcttttcaaa gtcggaaaaa tccataatca acgtagtggt attgatattt ggatgaaagc
    68761 ctacggtatc ttgacgatca atagcagcat cgatcacgac ttggattttt ttctctttgt
    68821 cgtgcatcaa tgctaatgga gtcactgtac ctgctggaac gcctaggagg tcaggaagct
    68881 gttcttcaga aacaaaagat aagcgctttt cttttaattg ttcagctagt tgttttaaat
    68941 cggcgtgctt ttcatcaggc aagatgacaa gatagatttg ttttcctttt cttgatttca
    69001 ataatagatt ttttacttgc ggaccaggta gggaaaaagg gacatttttg actgacgtaa
    69061 tcgcaggatg atcgactttt tgaaaagaga tacccagttt ttttagtagt tcataagctt
    69121 cttgttcggt tgcacgactc atcgcgcatc accctccttt ttttattagt gtatcataaa
    69181 aaagagttga caaatgaaaa ataatcatgt aaattacacg taaagttaaa acaaatgaca
    69241 tcgaaaagaa gagtagccgt taggtgtttt tagagagtct ctggttggtg gaaagaggca
    69301 gcagttaacc gtgaaacaca tcttggagtc gattttctga agcgtatagt aggaaaatcc
    69361 ggggagcccg ttacagctgc ggtcgttgct agatgaccag ataaggctgg cttttccgtg
    69421 aggagtcggt aaaaaaaggt ggaaccacgt gattaacgtc ctttgatctt cggatcaaag
    69481 gactttttgt tttcatctga acggcaacga ctgtgtgagg ttttttctgc aaggaaaaaa
    69541 taaacagagg tggaaccacg tataatacgt cctcttggca tttgccaaga ggattttttt
    69601 attttaaagg aggaaaagaa gatggagtat ttgattgaag ttatgccgca attattagta
    69661 ggagcactca caacattgaa attattttta tttaccttga ttggctcatt accattaggg
    69721 attgttttag cattaggatt gtctttgcat tttaagccgc tacgctactt gcttcaaaca
    69781 tatgtatggg ttatgcgggg aacaccgctg ttgttgcaac tgatttttat tttttatggg
    69841 ttgccgacga ttgggatcgt ttttgatcgt ttcgaagcag cactcattgc ctttatctta
    69901 aactatgcag cttattttgc agagatcttt cgtggaggga tacaatcgat tcctcaaggt
    69961 caatatgaag cagcgcaagt tttgcaatta agtccctttc aaacggtgac gaaaatcgtc
    70021 atcccacaag tgacaaagat tgttttacct tctgtaggga atgaagttat caacttggtc
    70081 aaagattctt ccttgatcta tatattaggt ttaggtgacg taatgcgtgc tgggaaaatc
    70141 gcgatggaac gcgacgcgac tttactgcct ttgctaggcg tggcaatcat ctatcttaca
    70201 ttgactggta ttctaacaat cgtaatgaaa caagttgaag gaaaattaaa ttattaccgc
    70261 taactgatag gaggaagaaa tatgattgaa ttgaagaaag tgaacaaagc atttggtgaa
    70321 aaaaaaataa tcaaagattt gaatctaatc attccagatg ggaaaacatt agctatcgta
    70381 ggtccttccg gtggcggtaa aaccacgctt ttacgtatct tggctggctt agaaagttct
    70441 gacagcggag aattttattt agacggtgta ccgttcaacc cattcgagct taaaatgaaa
    70501 gaacaagttg taggagtcgt attccaagac ttccagctat ttccacatct gaatattttt
    70561 gaaaacatta ccttagcacc aaaaatggta ttgaaacaga caaaagaaga atacaccaaa
    70621 aaagcagaag aattagcggc ttcattaggg attagtgatt tattgaatat ctatccttat
    70681 cagctttctg gcggacaaaa acagcgggta gcgattgcca gagcattggc tatgaatcca
    70741 cgggtattgg cttatgatga accaacaagt gctttagatc ctgaattacg ccagcaagtc
    70801 gagcgtttga tcctatcatt gaaagaagat ggggtaaccc aaattattgt cacccatgat
    70861 ttagtatttg cagaaaatgt cggtgatcaa ttactaaaag tttccccaat tcattaagaa
    70921 aggagaagat agagatgaaa aaaaccagtt ttctattcgc tttgatcgca ggtcttcttc
    70981 tttttgcagg ctgttcgaac aaaaaaactt cagcagataa ttgggcgact atcaacgaag
    71041 agaaaaaaat cgttataggt ttagacgata catttgttcc tatgggattt agagataaag
    71101 acgggaagct cacaggattt gatattgatt tagccaaagc agtctttgaa caatatggga
    71161 ttacggtaga ctttcagccc attgactggt caatgaagga atttgaactg aacaatggaa
    71221 cgatcgattt gatatggaac ggctattcca aaacgccggc aagagaaaag aaagtgcaat
    71281 ttacaaaacc ttatatggaa aacgatcaag tgctgatcac acctaaagaa tcaggaatca
    71341 caaactttgg acaaatgaat aataagagat taggtgcgca aaatgggtca tctggttatg
    71401 atgtatttac gaaacaacca gaagtattaa aagagatagt caaaaaccaa gatgctgttt
    71461 tgtatgatag ttttaatgaa gcattgatcg atctgaaaag cagacgggtc gatggtctgc
    71521 tgatggataa agtatacgca gactattatt tgaaacaaag aaacgaacta gaagacttca
    71581 acataacaaa aggttcttat gagagtgagg attttgcagt tggtgcacgc aagtcagata
    71641 gtacattagt gaaaaaaatc aatgaagccc tagatcaact agagaaaaca ggtgaattcc
    71701 aaaaaatatc cgataaatgg tttggcgaag atgttacgcc gagaaattaa caaatagggg
    71761 actgtgacaa aagacgtgtc acagtccctt tttccgaata aacagtgttc tcctgccaac
    71821 tccttgtgac tcggagctga atggcaggct cacaacctct tggtaaacgg tgttcaaaag
    71881 ccagtttttc ggtattaagc caaatttatg caaaaatgta gaaagccatt ttcacaaaat
    71941 ttttcttact actcgaaact aagcgacttt ttcacaacct cctttacata tttttatttt
    72001 gttgtggaag gggtttcgtg ttattctaaa agaggaacga ttgaaggtta taggaaagga
    72061 agagatgaat ggaacacaaa gaacttacct catttccaaa agattttctg tggggctctg
    72121 cttctgcagc gtatcaagta gaaggagctt ggcaagaaga tgggaaagga gaatcagtct
    72181 gggatcgatt tgtacggata cctggaaaga catttaaagg aacgaacggt gatttggcag
    72241 tagaccacta tcatcgttat aaagaagata ttgctttaat gaaagaacaa ggtctgaaag
    72301 cttaccgctt ctctgtagct tggacacgga tttttcctaa tggacgagga gaagtcaatc
    72361 aagctgggct cgctttttat gagagattga tcgacgaatt gattgaaaac gatattgaac
    72421 ctatgttgac tttatatcat tgggatcttc cacaagcatt gcaagatgaa tataatggtt
    72481 gggaatcacg tcagatcatt gaagatttca ctcattatgc agaaacatta tttgaggcat
    72541 tccgcgggaa agtccattac tggattagtt tgaatgaaca aaatatcttc acttcattag
    72601 gttatttgtt agcagcacat ccgccaggtg tgacagaccc gaaacgaatg tatgaagtta
    72661 accatattgc taatttagca aatgcatctg tcatcaataa atttcacgag ttagaaatgc
    72721 ctggaaaaat cggaccaagc tttgcgtata cgccaaatta tccaattgac agtaatcctg
    72781 aaaatgttct ggctgctgaa aatgcagaag acctgatggc caattattgg atggatgtct
    72841 atatgtgggg aaaatatccg attgctgcca tgaggttctt agaagaaaaa ggatgggcac
    72901 caacgattga agctggcgat gcagaattgc tagaatctgc taaaccagat tttcttggga
    72961 tcaattacta tcaaacggcg acgaatgcat tcaatccatt aaacggagtt ggtgctggaa
    73021 aaatgaatac gacaggaaaa aaaggttcat cagaagaaac tggagttcct ggaatgtata
    73081 aaaaagttga gaatccattt gtcgaacgaa caaattggga ctgggagatt gacccagaag
    73141 gattaaggat cggactgcgt cgtatcacaa gccgttaccg catgccggta ctgattacgg
    73201 aaaatggctt aggagagtat gacaagttga cagaagataa acaaatccat gacgactatc
    73261 gaatcaatta cttacaaagc cacgtcaaag ccatcaaaga agcaatttcg gatggagcag
    73321 aagtcctagg ctattgcacc tggtcttata cagatttgct tagctggctg aacggttatc
    73381 aaaagcgata tggatttgta tatgttgatc aagacgaaac acaagaagga tctttggaac
    73441 gatataagaa agacagttat tactggtacc aaaaactgat tgaagaaaat ggagagaacc
    73501 tttagttcac atacttaaaa caagaaggta cggctggata tggccgtgcc ttttcttgat
    73561 aaaatcattt agaaattaag aaaaactgta aaataagctg aaaaatgaaa gaaagcgtca
    73621 gttctttcaa acaaaacttt agttccctta cttttttttg tataatgaaa cttgatgaaa
    73681 tgagggataa taaatggcac aaaagaaacg gcctactaaa aaaaggaaaa agacgaaaaa
    73741 aacgaaaaag caaaaacagc agcaggaaca aatgctgacg attggtgttg ggtttgcctt
    73801 gattttattt gctgtcttcg gctttttaaa attaggtttt ttaggaatat tgattgcaaa
    73861 cggctttcgg atcattgcag gaaatactta ccaaatcctc tgtcttttat tagccgtatt
    73921 aggattttgg attgtcatta agaatacaga gttttcaatt ggtaaaaacc gacgctggtt
    73981 tggcggcata ctcttttatt tcggtatctt attattgtta catgcccatt tatttggcaa
    74041 actccatact ggggaaccaa atatcatggg gacgacatgg gatttgttag ccagtgatat
    74101 aaaacaaagt caagtagaca ataacgttgg tggtgggatg attggagcaa tcctttatca
    74161 ttttacttac ttcttgattg cacagcctgg aagttattta gcagcagtgc tattactggc
    74221 aggaggagca ttcctgttca gtaatctgga aggctatcag ctgttaaatg gtatccaatc
    74281 aattggagaa cgagtacagg aattattgga aggtgatcct gccaagcaag ctcgaaagca
    74341 agcggcaaag gaagagagaa tgaaacagcg cgcagaagcg aaagaagcaa ggcgcttagc
    74401 tgcaaaagaa gctgcagaaa aagaagctgc agaatatgag aagaaaaaag cggtgagcca
    74461 gcaaagggaa aatactgctg atgaatggca ggaaccagaa aatacagagc cggagcagct
    74521 aagttttgtt ccaatcgaca gttttcaaga aaatatccat cctgcgaatc tagagaaacc
    74581 agtaccagat acaccaaaac agaccaatac agcagaaggt ttttcagatg agttgccaga
    74641 ggatgacggt acctcattag aatttgagat tgaagcagaa caggaaaatc aagactatga
    74701 attaccgaca gttgatttgc ttgattctat accaactgtg gatcaaagtg atgagtataa
    74761 aaaaattgaa aaaaatatcg gcgtattaga acaaactttc caaagtttcg gtgtcgatgc
    74821 caaggtcgtc aaagccagct taggaccatc cgtcacaaaa ttcgaagtcc agccggctgt
    74881 aggggtaaaa gtaagtaaaa ttgtgaactt aacagatgat atcgcgcttg ctttagcggc
    74941 gaaagatgtc cggatggaag caccgattcc aggtaaatca ctgattggta ttgaagtacc
    75001 aaatggaaag atcagtatgg tttcctttag agaaatcatc gaagcacaac caaaccaccc
    75061 agataaatta ctagaagtac ctttaggacg tgatgtttct ggacgggtac aaacggctga
    75121 tctttcgaag atgcctcact tactcgtggc tggttcgact ggtagtggga agtcagtagc
    75181 aattaatggg attatcacaa gtatattaat gcgtgctaag ccccatgaag taaaactgat
    75241 gatgatcgat ccgaaaatgg ttgaattgaa tatgtacaac ggcatacctc atctgttgac
    75301 gccggtcgtg acgaacccta gaaaagcagc gcaagcctta caaaaagtag tacaagaaat
    75361 ggaagaacga tatgaaaaat tcgcggcaac cggagtaaga aatatttctg gttacaatga
    75421 atttgttcaa cagaaaaact tggaaaacgg aacgaaacat ccaaccttac cattcatcgt
    75481 agtgatcgta gatgaattag ctgatttgat gatggtagcc agcaatgaag ttgaagatgc
    75541 gattattcgt ttagcgcaaa tggctcgggc agcggggatt catatgatcc tagctactca
    75601 acgtccaagt gtggatgtta tcacaggaat catcaaagca aatgtacctt ccagaatggc
    75661 atttgcggta tcaagcggta cagattctcg gacgatcatt gattctaatg gggcagaaaa
    75721 gctcttaggg cgaggagata tgctattctt gccaatgggt gagaataaac cgatccgtgt
    75781 gcaaggagca ttcatttctg accacgaagt agaacgggtc gttcaatttg ttacagatca
    75841 gcaagaagct cattatgaag agaaaatgat gccgacagat gaagttgaaa cgacaggtgc
    75901 tccagaacag ccacaagatg agctgtttga agaagcaaaa gcacttgtcg ttgaaatgca
    75961 aacggcaagt atttcccttc tccaaagaag atttcggatc ggttacaacc gagcagcacg
    76021 tttagtagat gaactggaag ctcatggagt tgttggcccg tcagaaggaa gcaagcctcg
    76081 gaaagtattc attgagcagg aagaaacatc agaaacgttg ccggacgata atgacatgta
    76141 aaacttgtgt gatgaagggt tgaaaaacga ataaccaata aaaagacaag gcatgatgat
    76201 accaaaagaa aaaatggttt ttttccatct tttctaatac ggtatgatgt cttgtctttt
    76261 tagtttttgt gagattaaag ttctattcga tttatttgtg aagtattgca ttaaggaaat
    76321 gaatcatgta tagtatgtag cgaaactttg agaaaaagag ggaaaatact ttggaaaaac
    76381 agtccattta tggcttgacg aatgaagaat taatcaattg gtttatagaa aacggcgaga
    76441 aaaaattccg agctgcccaa gtttgggaat ggttgtatca aaaacgtgta agtaatttca
    76501 cagaaatgac gaatctatcg aaacaattga tcgaaaaatt atcggagcat tttatcatca
    76561 atcctctaaa acagatggtc gtccaagaag cttcggatgg tacagtaaaa tatttgtttg
    76621 aactccctga taaaaacatg attgagactg tactgatgag acaagaatat ggcttgtcag
    76681 tttgtgtgac gacacaagta ggatgtaata tcggctgtac gttttgtgca agcgggctgc
    76741 tcaaaaagaa ccgtgatctg actgctggag aaattgttgc acagatcatg atggtccaac
    76801 attattttga tgagcgcaat cttggcgaac gcgtttcaca tgtcgttgta atggggatcg
    76861 gtgagccatt tgataattac gacaatgtaa tggacttttt gcatatcatc aatgatgcca
    76921 aaggattggc aattggtgct cggcacatta cagtttccac tagtggatta gcacacaaaa
    76981 tcaaggaatt cgctaataac ggcttgcaag tcaatctggc tatttccttg cacgcgccga
    77041 ataatgaggt gcgaacatcg atcatgcgga tcaaccgaag tttcccaatt gagaaattga
    77101 tggaagctgt ggatgagtat ttagagaaaa caaaccgtcg aatcacgttt gaatatatca
    77161 tgttgaatca agtaaatgat agaccagagc atgcgcaaca attagcagat ctattaaaag
    77221 ataagaaaaa attagcatat gtcaacttga ttccatataa tccagtcagt gaacatgatc
    77281 aatattctcg cagtccaaaa gctgatgtat tgagatttta tgatgtattg aagaaaaacg
    77341 gcgtaaactg tgtgatacgt aaagaacatg gtacagatat tgatgcagct tgcggacaat
    77401 tgcgaagcaa gcaaatgaaa aaaacagttg ccaaataatc gtatcctact cgttttttca
    77461 attaccataa aaggagcagt tttcttaatt aaggaactgt tcaacttaaa taagagaaaa
    77521 gattctatag cgaaagctat aaagcttttt ttgacaagaa aaaccctcat caagcaaaga
    77581 aatgtcctca atttgatgac agcctgcttg atgggggttt tagcaattat ttaactgatt
    77641 ttcttatatc cttgctggct ttcttgtgta gcatgataag tttctacgtc aagaatggcc
    77701 tgtttaaatt tttctcgggt gatttcataa ggcacatgtt taatatcatt tgttgccatg
    77761 gctttatcta agattgcttc taaatcggtc gtgacattga gattcatgtc ggttaacgat
    77821 actggaagtt ggttcttttt cataaacaca tagattttat caaacaaatc gaactgctga
    77881 tccatcatca ataaggtcag tacaccataa ctgacagatg ctccgtgaag gtatccctcc
    77941 attttttcta gaacagtcgt accgtaatag aatgagtgtg ccatagcaga attatagtta
    78001 ttgttaacta aaacagaaac caatccggta gtaacaatga tatccgaaac gacttgttca
    78061 attgcatatg agggacgatc gtcggcacta tctttcaatg cttgcgtacc ataagtcaag
    78121 agacgatcaa tgcacatttt tccgatttgg attcctaatc tatccgtgta gcctagatca
    78181 tcgccacgag cagaaaaagt agattcgact tctttggata aagcatcgcc aatacctgcc
    78241 cataagtatt tatcaggtgc ttcggcaata attgctgtat caatgaaggt gtgtacaggt
    78301 ggttttgaag gaaagaagag tccgtccatt acgccttctt cgttatagat gacacatacc
    78361 gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg tgaagactgg tttagctagt
    78421 ttttcaccaa tgacttttac agtatcgcaa gcacggccac caccgactgc aaacaggacg
    78481 tctgcttttt ggacactttc atgctggatc aactgttcaa tatttccata agttgaatta
    78541 cccccgtacc agagactggc gacaacctct atttcctgtg tcaaagctac ttttattttg
    78601 ttttctgcct ttgaaagtgc agtcttccct ccaatcagca ctacttttgt cccatattct
    78661 ttgacgattc catttgcttt ttcatacgca tctgttccaa tggtataatt tggtaagaaa
    78721 acactttgac tcatattcat ttccttcttt cttcgttgaa acgggttaaa attttaaaga
    78781 gtgatcgatc tgactgcggc tgtagatgga gtagaggtga acctaagaca ggataagtga
    78841 tgaataaggt ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa cgaacaagta
    78901 caaaaacatt gaaaacgaca ataatgaatt tttgttcctc ttagtatttc taccgattcc
    78961 ctttgctttt ttgtaattat ctaaaaattc taaaaattaa gattattata tgaaaacagg
    79021 ttttatttgt caattcataa ttggtttttt ttaaagcaaa agataatcta tatcatgatg
    79081 aacaaacaga gttttttcct ccaattgatg gctttcctga tctttcctat tttgaataaa
    79141 gtgtttctgt ataatttttt tattttataa gtaagataaa taatgaaaga atagagatac
    79201 tgattggtag ctaaagattt ttgagcgaga acatttcgtt ttttcaaaag gactgttaga
    79261 ataagcaaga acaaggggga agacgtatga taatcagtga atttgaccgt aataatccag
    79321 tattgaaaga tcagctttct gatttactga gactgacttg gccggaagaa tatggagaca
    79381 gctcggcaga agaagtagaa gaaatgatga atccagaacg aatcgcggta gcagcggtag
    79441 accaagatga gttagtagga tttattggtg caatccctca atacggtatc acaggttggg
    79501 aattgcatcc attagttgta gaaagctccc gacgaaagaa ccaaataggt actcgattag
    79561 tcaattactt agaaaaagaa gtagcttcca gaggaggaat cacgatttat ttaggtacgg
    79621 atgatttaga ccatggaaca acgttaagtc aaacggacct gtatgagcat acatttgata
    79681 aagtggcttc catccagaac cttcgtgaac atccgtatga attctatgaa aaattaggtt
    79741 ataaaatcgt aggtgtctta ccaaatgcaa atggctggga caaaccggat atttggatgg
    79801 caaaaacgat tattcctcga ccaaattctc aataataaaa agaggttgtg acagaagtaa
    79861 tcagcttcaa gaaataagaa ggaatttacg aaaattactt cttgaatttt tgtgaaattc
    79921 caacttattt ccgaagaagc tacttttgga acaccgtgta ccagaggttg tgaaaaagtc
    79981 gcttagtttc gagtagtaag aaaaattttg tgaaaatggc tttctacatt tttgcataaa
    80041 tttggcttaa taccgagaaa ctggcttttg aacaccgttt attcagataa gggactgtga
    80101 caagactttt gtcacagttt ctttttgttt attctttttt atctttgtat ggcaataata
    80161 gacaatataa cgaaaattat ttaatggctt ttggaagacc atggaaaacc ttgtacaaag
    80221 ctattttttc tgaaaaattc cttgaaacct cacgaaaaaa tcattatttt atgaaaaaga
    80281 caaaaaaaag tgttgatttt ttaataatta ttcattaaac tcttaaacaa cttcagaaaa
    80341 tattaattat tctgaaaatt ccaataaagg ggtacgtaaa atgaaaaaga aatttacatt
    80401 tggtttggtt gcggtatgcg gccttgtact tgctggatgt tatggcggca gttcagccac
    80461 aagcagtagt tcagcaaaaa gcggttcagc agatggagga ggcgtattta acttagtcgt
    80521 gcctcaagaa atgcctacag ctgacttatc agttgcaaca gatacaatca gttttacagc
    80581 attgaataac gtttatgaag gaatttaccg tcttgatgaa gacagcaaac cacagccggc
    80641 aggagctagc gagttggcag aagtcagtga agacggatta acttacaaaa tcaaattacg
    80701 agaagacgca aaatggtcaa atggtgatcc tgttacagca gcggattacg tttatggctg
    80761 gcaacgaaca gtagacccag ctacagcttc tgaatatgct tacctgtatg cgccagttga
    80821 aaatgcagaa gacattacag ctggaaagaa agataaatca gaactaggga tcaaagcagt
    80881 tggtgactat gaattagaaa tcaaattgac gaaacaaaca ccatatttcc aatatctgct
    80941 tgctttccca tcatttttcc cacaaaaaca atccgtagtg gaagaaaatg gggatgccta
    81001 tgcatctgca agtgataaag ctgtttataa tggaccattc acattagaag attttgatgg
    81061 accaggaaca gatacagaat ggacttataa gaaaaatgat cattactggg acaaagatac
    81121 agtaaaactt tctgaaatca aagtcagtgt agtaaaagaa tcttctactg cattgaactt
    81181 attcaaagat ggccaagcag atgacgtgat cttgtctggt gagttagcac agcaaaacgc
    81241 aaacgatcca gcttatactt ctgttaaaga agcgcggaca agctatatcg aattcaatca
    81301 acgtgaaaag aattcaccat tcaacaatgt gaacctaaga aaagctattt cttattctat
    81361 cgatcgtgaa gctttagtaa aacaagtact aggtgacgga tcagttgtct ctacaggatt
    81421 gatcccatca gatatggtaa aaagtccaac aacgaacgaa gactttgcaa aagaagctgg
    81481 aaaattagtt tcttatgaca aagataaagc aaaagaatat tgggaaaaag cgaaaaaaga
    81541 attgggcgtt gattctttag aatttgattt gatggcttct gatgatgatt catcgaaaaa
    81601 agtaatcgaa tatgttcaaa actcaatcca agaaaacttg gatggcgtaa cagtcaaacc
    81661 aacacctgtt ccattctctg tacgtcttga ccgctcaact tcgggtgatt ttgacgcagt
    81721 actaggcgga tgggctgctg actatgcaga cccaagcagc tttactgatt tgtttgtcac
    81781 aggtaattca tacaaccgtg gtcgctggag caatgcagaa tatgacgaag cagttaaagc
    81841 agcttcatca acagacgctg gcgatgaaca agcccgttgg gatgatttcc aaaaagcaat
    81901 caagatcatc tctgatgata tgggtgtcgt tccggtgtac caaaaagcag aaggtcacct
    81961 agtgaatgaa aaaatcaaag gtatcgttca ccatgcggca ggcgcttcat gggattacaa
    82021 atggacatac gttgaagaat aatcgttaat accgtgagca tgagaaaaaa cgaggctgcg
    82081 gcaaatgtcg caacctcgtt ctcatattca cttatcctaa caaagaaaaa agcatgaaaa
    82141 tagcagaaag agcaattagt attccggcaa tgatcagcca aaagctgtac ataccgtagc
    82201 tggcagacga gagcgaggaa aattctggtt ttttctttcg gttccttgtt cgtgcaagat
    82261 ggaccgatcg ctgaaaatga tcgaagaaac ccgaagaaaa gacccataaa aaaccgccga
    82321 taatcagaaa cggcaatgac cataagaata gatcattaga taattgaatg agtgtaaagg
    82381 aatggttgat gagatttttt attaaaataa ccagaatcag tagaaaagct actgcgtagg
    82441 ggatagattt ttttttcatt tgattgctcc tttatttttg gttcattatc taataatgta
    82501 gcgaaacatc tgtcagaagt caaaagctag ggggaaacat tagtgaatag ttatatcaaa
    82561 tatgtattaa aacgtgtctt tttcatgatc atcacgcttt ggttaattgc aacaatcacc
    82621 tttttcttga tgcaattgct gccgggcacg ccatatacga atcaggaacg tttgagtcct
    82681 gaaacaatcg agatgctaaa taaacaagtc ggtttggata aaccagttat cgtacaatat
    82741 gggatttatc tttctaattt gcttcaagga gattttggaa tttccttcca atttaaaaac
    82801 cagccagtcg cacatttgtt agctggaaga atcggaccat ccttgcagtt agggttacag
    82861 gcaattatct ttggaacagt cctagggacg attttaggaa cgatctctgc catgaagcaa
    82921 aatacatggg cagatacatc ttctacactc gtagcgatat taggtcgttc cattccaaac
    82981 tttgtatttg ctgttttatt acaatatatc tttgcaatca aattacaagt tttgccaatc
    83041 gcaaaatggg atggctttgt gtacacaatc ttaccaacaa ttgcgttggc catgtcgccg
    83101 cttgctgact ctgctcggtt tatccgtacg gaaatggtgg aagtactgca tagtgattat
    83161 gtggaattag caaaagcaaa aggattgagc cgttgggaaa tcgcctttaa acatggtctt
    83221 cgaaatagtt tgattccatt gatgacttta ttaggaccgt tagctgtagc attgatgacc
    83281 ggatcactgg tggtagaaaa tatctttgct attcctggta tcggggaaca attcgttaag
    83341 tcaatcacaa caaatgatta tcctacgatc atggctgtaa cgatccttta ctcatttatg
    83401 ctgatttttg ttattttgat cgtggatcta ttgtacggtc tagttgatcc aagaattcgt
    83461 gtttcagaag ggagccgagg ataaatggaa atgataccaa agcgttatga aacagttgca
    83521 gatatccctg cggatgaatt tcttccatta caaaaaaata ccgaagaaga acgtgaacag
    83581 attactgcac cttctttgaa ttttatccaa gattcatggc gccgtttaaa gaaaaacaaa
    83641 gctgccgtta tttcaatggt tttactattg gtcatcattt tgatctctgt gatcacaatc
    83701 tttgtttctc ctcatgatcc aactgcccaa aacgtggctt atatcaattt gcctcctcgt
    83761 attccgggaa tcaatatcga tggattgaat ggaaaagcaa tggttggtgg tgaacttgtt
    83821 gataaatatg ctcaagcaaa cgtcccttca aacgtaaatt tctttttagg aacagacggt
    83881 ttgggacgtg atgtattaag ccgcttattt atgggaatga gaatctcgtt gctgatcgca
    83941 tttattgctg cattactaga tgtaacgatt ggtgtagctt acgggttggt ttctggtttg
    84001 ttaggcggac gtgtcgataa cgtgatgcaa cgttttttgg aaatcttatc aggaattcca
    84061 aacctcgttg tcatgatttt gatgttggtc gtttttgaac caggaatttt ttcaatcgtg
    84121 gctgctatgg ccatcacaaa ctggatacca atggcacgta tcgttcgtgc acaaacaatg
    84181 aaattaaaag atcaagaata tgttttagct ggattgacat taggtgaatc aagaatgaaa
    84241 attgcattca aacatatttt gccgaatatc tctagtgtca ttattattca aatgatgttt
    84301 agtattccat ctgctatttt cttcgaagcc tttttaagct ttatcggttt aggtcttacg
    84361 cctccgtcag cttcattagg aacgatgttg agcgaagggt acaagacatt cctgtatctg
    84421 ccttatctat tatggattcc ggcagtaact ttatcggtta tcatgattgg ctttaactta
    84481 cttgctgatg gattgcgtga tgcctttgat cctaaaatga aagagtgaag acgatgacga
    84541 aaattttaga agtgaaagac ttagaaatct cttttgatac gtatgctgga aaggtacgag
    84601 cgattcgcgg tgtcaatttt catttgaata aaggagaaac attggcgatc gttggggaat
    84661 ctggaagtgg aaaatcagtg acgaccagaa gcatcatgcg cttgctgtca agcaacgcca
    84721 atatagatgc tggtcaaatc ctctttaaag gtaacgatat cgttggaaaa tcagaaagag
    84781 agatgcaaaa aatccgtggt aaagaaatag cgatgatttt ccaagatcca atgacctctt
    84841 tagatccgac gatgcccatt gggaagcaag tcgctgagtc ccttagaaag cataataaag
    84901 tttcaaaaaa agagggacaa aaagctgcat tagaattgct taaattagtt ggaatacccg
    84961 aagctgagaa acgtatcaac agttatccgc atcagttctc aggaggtcag cgtcaacgga
    85021 tcgtgatcgc gattgcactg gtatgttatc cggaaatcct gattgctgat gaacctacaa
    85081 cagcgcttga tgtgactatt caagcacaga ttttggagct gctgaaagaa attcagacaa
    85141 aaatcgatac atcaattatc tttatcactc atgaccttgg tgtagtagcg aatgtcgctg
    85201 atcgtgtcgc tgtcatgtat ggaggaagaa tcgttgaagt tggaacatcg gaagaaattt
    85261 tctacaaccc gcaacatccc tatacttggg gactgcttgg ttcaatgccg acattagata
    85321 gtgcgaacga tcgattgtat gctattcctg gttctccacc ggatttattg aacccgccaa
    85381 aaggagacgc attctatcca agaaatgaat tcgcgatgaa aatcgatgca gaacaagaac
    85441 caccattttt tgaactatca aaaacacaca aggcagcaac ttggctactt gctccgcagg
    85501 cacccaaagt gacaccgcca gaagaaatcc aacgtcgctg ggctatcttt aaagagaaac
    85561 aaaaacatac ttacggtcaa ggaggaattg ctgatgccaa ataaactact tgaagttaaa
    85621 ggattgaaac agtatttcaa tgtaggaaaa aagaatgaag tacatgctgt aaatgacatc
    85681 agctttcaca tttatgaagg agaaacattc ggtttagttg gagaatctgg aagcggaaaa
    85741 tccactactg gccgtacgat cattcgcttg aatgaaccta ctgatggtga gatattattc
    85801 gacggtcaag atataacgaa gatcaaagat aaaaaaggtt tgacgaaatt ccggcatgat
    85861 gttcagatga tctttcaaga tccatacgct tcgttgaatc ctcgtatgaa agtcagagat
    85921 atcattgccg aaggaattga tgttaatgga ctagctaaat catctgaaga gcgtgcaaaa
    85981 aaagtcgatg atctattgag aactgttgga ttgaatccaa gccacggtac tcgttatcct
    86041 catgaatttt caggtgggca acgccagcgt attggtattg cacgagcatt ggcagtaaaa
    86101 ccgcgtttta tcatttgtga tgaaccaatt tctgcactag atgtgtcgat ccaagcccaa
    86161 gtggtcaatt tgttgcaaga tctccaaaaa gaacatcaat tgacctatct atttattgca
    86221 catgatttgt cgatggttaa acatattagt gaccgaatcg gtgtaatgca taacggcttg
    86281 ttattagaga tgggaacaag tgatgaaatc tataatcacg gtgttcatcc ttatacagaa
    86341 agtttgttat ctgctattcc tctaccagat ccagaccatg agagaaaacg cagaagaatc
    86401 aaatatcaac ctgaaccaga tgacggacaa gtacgtcaac ttcgtgaaat cgcaccagaa
    86461 cattttgtct atgcaactga gcaggaagtc ccatattacg caaaaaaact aaaacgacaa
    86521 aaggaatctc ttctggttgc taactaggag ggacatagat gctaaaagct tttaaattcc
    86581 gtatgtatcc gacagaagaa caaaaacagc aattgattcg tacttttgga tgtgctcgat
    86641 ttacctataa ccatttgctg aaaaagcggc agaaaagttg gcaacaaaca ggagtagcca
    86701 atttttcgct cacacccgct acattaaaaa aagaataccc attcttaaaa gaagtagaca
    86761 gtcttgcttt agcaaatgca cagcttaatt tggatcgggc ttttcgcaat tatttcaagg
    86821 gacgtgccag cttcccaaag cttaaaacga aaaaaagtat gtggcaatct tatacgacca
    86881 acaaccaaac ccgtacgatc tatctggaaa atggctactt aaagttacca aaacaaaaag
    86941 aactaatcaa gataaatcag catcggccag ttgaaggatc gattcgctcc gctacgattt
    87001 ctgctcgata taatgaagaa ttttatgtcg cactactttg tgatgtttcg ccagtaaaaa
    87061 aagaatcttt agctaaatgg atcgggatcg cctatcatcc gaagacattg atccaaacat
    87121 ctcgacctct agaagtaact ttaccaaaat ttcaccagac agaagaaaag ctgcaacatg
    87181 cacagcggaa attgaatgtg aaagtccgct ccgctcacca tagaaagatc aggttagatc
    87241 aggcaagcaa ttatcagaaa caaaagagaa aagtaatgga tctttatctg aaacaaaaga
    87301 accagcgaga agattatctt gaacaattat ctgggaaatt ggtcaaacaa tatgactact
    87361 tatttgtaga atcttttcca aaagaagagg cacatgctga tttttctatt cacgactggc
    87421 ataaattaat aacaaaattg cgttataagt cccaatggta taataaaaaa tttcttttga
    87481 tcaataccga cggagcagaa gaaagcaatt ccgtaagaaa gagccaagta gtagaaaaaa
    87541 tgggcagaca ttcggttatc aagggataaa aaatgaatga aactgatagg agcgttaggg
    87601 ttagccttgt taataaaaag tcttaatgac ttttgtttct aggaattttt cttttgaaga
    87661 aaagctcact aattatgaca aaccaataaa acaatagcag aaaaagttcg tacgagcttt
    87721 ttctgctatt gttaattttt tattcagaaa gtttacgaat acatcaaaat tctgaaacaa
    87781 aaaacgtaac tcacaaagag ttgtttttgc gttttttctt gaatgattag gtctgaatct
    87841 tttgctcaca accttattca tggtgccccg gcttgagctg atagattttt gaaggacggc
    87901 cgattcctac aggacgttca ccgatctcat cgaaatattg gagcatagct ttcttaaaat
    87961 tggaatgatc gatcgtttta taatcgatcc ctaagaattt agcaaatact tttcgagctt
    88021 ccgtaatcgt gaaatcttta cctaaaactt gtagaacttg cggctcgtgt tccattttat
    88081 tgactacacg gttgaatgct ttcaagatga tttgactatg gtcaaaagcc aaagtatctt
    88141 tgccagaaga ctctccagtg actaaatcaa gcgagatagc cacttctccg ctagataaat
    88201 ggatcttgtt actatgacgt tctagtgtga accagcgtac ttccttggca tcatctccgg
    88261 cgattagcgg ttcttctcca ataaaggcta agtagctgac tgtgacgacc cagcctcgcg
    88321 gatctcgatt aggcgtactg aatgtatgca gttgctcaat attttctttt gaaataacca
    88381 caccagtttc ttcctttgtt tctcttaaga cgctttctcc agttgactca tccttttgga
    88441 caaagccacc aggcaatgcc catgaatttc gataaggatg cccttttctt tggatcaaca
    88501 aaactttcag ctgatcttct tctttgttat agcacatcag tacgatatcg acagtcaaag
    88561 agggcttgtc atattctgga cgttcctgtt tttgatacca gttaagaaat tcttcttcac
    88621 ttgctttatg ttcataatat aatttttctt cttctttaga agcaaaacgg ttcatctgac
    88681 cacctccatt gacagtaaaa gtacttttta ccttattgta gcagactctg aaaaaaaacc
    88741 aaggatttac ttgaaaaatc taggttatca tttaaatatg aaaaataaat ggaagtatga
    88801 gattgaataa aaagaaaagc agataaaacg gcaatacttt ataccgctct tcacgaatgg
    88861 caaagaagcc acaaataacg atatatacgt caatgataaa acgcaaaatc caagctgctg
    88921 tcataaaact gttcaaatgc ccatggaggt taaaataacc atacatacga atataaaaat
    88981 agctgaatat taaatgtgct aatgaaagaa aagcaatact aagtgtaaaa aaaagatgtt
    89041 tcttctgcat gattgttcca gtcccttact aagatagata gtctttatta taccacgtgt
    89101 gaggggagaa ataaaaagcc gttttttagt agatatgaaa caaaacaaaa acccgcattt
    89161 attcaaccta ttcatccttt taaaggatag attgaacgta gatagaagtc gtttatccag
    89221 tctttttcat tttgaaaaaa agctaacagc atgctttttt tatggtatac tagcaaggaa
    89281 acgaattcgc atcatgcgat aaaggaggaa gagccccgtg gcagaaaagg aaacttttta
    89341 tatcactacg ccaatctatt atccaagcgg caaacttcat atcggcaact cgtatactac
    89401 gattgcatgt gacgcaattg ctcgttataa acggttgatg ggctttgatg tgttttatct
    89461 cactggagtg gatgaacatg gtcaaaaaat cgaaacaaaa gcagaagaac taggtgtaaa
    89521 accgcaagaa tatgtagata aaatggcggc tgatgtcaag aaattatgga aaacgcttga
    89581 tatcagttat gataaattca ttcgcacgac agatgattac cataaagcag ctgttcaaaa
    89641 aatatttgat cgtttattag aacagggaga tatttactta ggtgaatatg aaggctggta
    89701 ctctgtttct gacgaagaat tctttacaga aacacaactt gcagaagtgt acaaagatga
    89761 cgaaggtaac gtgatcggtg gtaaagcgcc aagtggacac gaagtagaat tggtaaaaga
    89821 agaatcatac ttcttccgca tgagcaaata cgcagaccgt ctattggaat actatgaaga
    89881 acatcccgaa ttcatccagc cagaatctcg taaaaacgaa atgatcaaca actttatcaa
    89941 accaggatta gaggatcttg cagtttcacg aacaactttt acttggggag taccagtcaa
    90001 gagtgatccg aaacatgtgg tgtatgtatg gatcgatgca ttatccaatt atattactgc
    90061 actaggatat ggctccgaag atgaaagtct attcgaaaaa tactggcctg cagatgttca
    90121 gatggtcggt aaagaaatcg ttcgctttca tacgatctat tggccaatca tgctgatggc
    90181 ccttgatttg cctctaccta aaaaagtatt tgggcatggc tggctattga tgaaagacgg
    90241 aaaaatgtct aaatcaaaag gaaatgtcgt ttatcctgaa atgcttgtgg aacgttatgg
    90301 tttagatgca ctccgttatt atttgcttcg tgctgttccg tttgggtcag atggtgtctt
    90361 tacgccagaa gattttgttt cccgtgtcaa ttatgatctg gcaaatgatc tagggaactt
    90421 attgaatcga acgattgcta tgatcaacaa atattgtgat ggactcgttc ctgactatgc
    90481 ctcacttgtc actccatttg atagtgaact gtctacgacg gctgctaacg ttgtcggacg
    90541 ttaccatgat gcaatggaaa aaatggaatt caatacagca ttggctgaaa tctgggtgct
    90601 gatttctcgt gcaaacaaat acattgatga aacagaacca tgggtattag caaaagacga
    90661 agaaaagaaa gctgaattga atagtgtcat gatccattta gctgaatctt tgcgtatcgc
    90721 agcgatatta cttcagccaa tcatgacaga aacgccagag aagatcttca atcaactagg
    90781 attggactct gaaacaatga atcttgaagg attacacttt ggcgaatttc cttcaggaac
    90841 aaaagtagtt gcaaaaggta caccaatctt cccacgttta gatatggaag aagaagttgc
    90901 ttttattcaa gaaaaaatgt ctgaaggtac tcaaacaaac gaagatacag tcaaatggga
    90961 tccagaagaa acagaacttg tttcaacgaa agaaaaacaa atcaaatttg atgtatttga
    91021 aaaagtagaa ctaaaagtag cagaagtcat caactgtcaa aaagtagaag gggcagataa
    91081 gttgctgcaa ttccgactag atgctggaga cagccaagac cgtcaaatct tgtcaggaat
    91141 cgctgaattt tatcctgatc caagcgaatt gatcgggaaa aaagtcgtca ttgtagcaaa
    91201 tctgaaacca cgaaagatgc gtggacaaat cagtcaagga atgatccttt ctgcagaagc
    91261 accagatggc tctttacaag taatcgaagc accaaaatcc atgccaaatg gctcagaaat
    91321 cgcatagaaa ttcaaagggg gtatgacact agttgtgtca tacccccttt ttccgaaatt
    91381 tgagaagcaa ttttcgtaaa ttccttctta ttacttgaag ccaatcatat aatacagaaa
    91441 cagagaacta tccaaaataa atcctagaaa aagaagtacg agcttcgctg atttttgtta
    91501 atttagaata atactaatag gaatcgtaaa aacagaaaaa aagcataatg ataaagggtc
    91561 gggcagattt taattgattc atctggtact cttttttatt gtccttacat tcataaagtt
    91621 tgaatgcaat gactttatta catctatatt tatatctaac agtagcgttg atgtgttaat
    91681 tataagttct atcactttat gttcaattag tttgtgctct tatatgtgtg attgaaaata
    91741 tagataaaaa aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa
    91801 tcctatcact tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga
    91861 gcagtaagca gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca
    91921 taatgttgat agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag
    91981 ccagctataa accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa
    92041 ggatagaaat aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg
    92101 atattcaata aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc
    92161 aaaaaggaat acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt
    92221 gcgatgaaca aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca
    92281 aataaaatta atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg
    92341 acccggttgg ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt
    92401 tcctccttcg tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc
    92461 ttaagaggga gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca
    92521 gttatactga aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac
    92581 taaaacgaga aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg
    92641 caggcgtctt cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat
    92701 tgctagcttg gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat
    92761 tagctacagc aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa
    92821 aattgcccag ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg
    92881 ctgcattatc gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt
    92941 atcttttgtt gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga
    93001 caaaagtcgg tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg
    93061 tgattgtcat atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg
    93121 aagctggaaa agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt
    93181 ttgcttatga cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa
    93241 aagatttgcc taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga
    93301 tcaattttgt ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag
    93361 cttctgaagc atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga
    93421 tcttgatctc ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt
    93481 atgcgatggc tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga
    93541 agtttacggt tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga
    93601 gccttggttc tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt
    93661 tactgatttt tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat
    93721 ataaagtacc actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt
    93781 taggtatgac gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga
    93841 taggtatccc agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa
    93901 caacagacag aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat
    93961 aaaacaagtt cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag
    94021 taagaatgaa aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa
    94081 cagttcaatg aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa
    94141 atggcggtcg tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac
    94201 tatccaaata tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa
    94261 gaaatcgaaa ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa
    94321 attggcttgg attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc
    94381 agacagattg ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca
    94441 cttgaagaca cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg
    94501 catagtttca gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt
    94561 tcatttagcg gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat
    94621 gtaccaatcg aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt
    94681 agaggaaagc gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt
    94741 cggaatcttc cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt
    94801 ggtctaagcg aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga
    94861 cgatacaaga aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc
    94921 aatcaatgaa gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt
    94981 ttttaccgat cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc
    95041 tcaggcaaaa catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct
    95101 tggtgtcgag catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc
    95161 agacatttcc caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt
    95221 aatagctggg gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg
    95281 ttacacgaac ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga
    95341 actcgcccaa gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga
    95401 caccctctag aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag
    95461 gacaaaattt tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag
    95521 atgaacatac gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag
    95581 ccaagcatgc aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg
    95641 ctgatacgat gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg
    95701 atttgtcaac agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga
    95761 atttacctta ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg
    95821 tggatgaact agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag
    95881 gaacgaaagc ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag
    95941 cattcatcgt accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga
    96001 aactgacaag aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat
    96061 tgacaaaagc ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt
    96121 atggcaaaga tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg
    96181 atccaaaacg tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga
    96241 tgtcagaaaa tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg
    96301 catcaagtaa taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc
    96361 ttattaccgc agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt
    96421 ttaactctaa gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt
    96481 ggtcttatta cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac
    96541 aaattttgtc acatctccta tttttgtttc ttgatcaaaa aattcttata cttgatactc
    96601 acactcattt tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag
    96661 ttatactaag tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa
    96721 attcagttcg aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa
    96781 tggtcatgcc taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat
    96841 tatttattgc gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat
    96901 ttggtttctt tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt
    96961 ttgcgttgat tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac
    97021 agtttacttt tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt
    97081 ctttagcaga aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat
    97141 attattctca aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc
    97201 acagtttagt tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg
    97261 atatccccgt atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg
    97321 cgaagaaaac gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga
    97381 taataaatcc tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg
    97441 gcacagctct aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca
    97501 aagaagtggc ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa
    97561 tctcttgcat ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt
    97621 tcagctccaa gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct
    97681 tcaagggcta cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt
    97741 ccttcataac ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc
    97801 ccatgtccat ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga
    97861 gatccaataa agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta
    97921 ccgatttgtt ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct
    97981 tctgtctgat cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca
    98041 atctcagcac gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt
    98101 aagaagactt ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca
    98161 ccagataaag cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca
    98221 ttgtccacac gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc
    98281 atattatcaa aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata
    98341 tcacggtctt taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca
    98401 gagatatctt ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga
    98461 ccaacaaaaa cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa
    98521 ttttctgcat tgtcatattt tttatatacg tttttcagag ccatttctac cataatacgt
    98581 tccacacttt cttttttagt tttattaact atttaaagta taaatgaaag cgttaactaa
    98641 cttcaatgtc atgttgaaca agaatttttt cttttttttg tcaatgtgct agttttaagc
    98701 tttaataaat atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca
    98761 atatcaaaag gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct
    98821 atgatggtga aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct
    98881 accgggacga ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa
    98941 tccggaccgt taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat
    99001 ttggtgattt ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca
    99061 cttatccgtt tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag
    99121 gtgctactaa gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata
    99181 gacaagcatc cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg
    99241 cctatatgta gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt
    99301 ggataatata aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat
    99361 agtttctgtg ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct
    99421 aagaggatgg gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca
    99481 ttcgctgcat gatacgatgt agcagctctt aaaggagatg ctaaaaatga agaaacaccg
    99541 gttagacgca cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc
    99601 ccgactggta gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt
    99661 gatcatgggc attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt
    99721 agtaggaatg gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt
    99781 tattgaagga gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc
    99841 cattctggct acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct
    99901 tgcgttgatg taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa
    99961 aacagtaata tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc
   100021 ttacaaaaaa attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc
   100081 catcccagaa gactgcttgt ttttattgtg ctgtgaattg tgcttgatag agccgtcggt
   100141 aataaccatc tttttgaagc agctcatcat gggtgcccat ttcgacaata gctccctgat
   100201 ccatcactaa tatctggtcc gcatttttga ttgtggacag gcgatgggca atcacaaaac
   100261 tggtttttcc ttccatcatt gctaagaagg cttgttggat ttttttctct gtcaatgtat
   100321 cgactgagct ggtagcttca tctaagatca acataggggg attactgatc atcgttcgag
   100381 caatcgtcaa cagttggcgt tgtccatctg atatctttaa cccttgaccg ccaatcttag
   100441 tatctaattt ttccggcaaa cgttctacaa attcatacat ataagaggct tttagggcat
   100501 cattgatttc tgcgtcactt gcttccggat tcccaaaacg aagattttct cgcaatgtac
   100561 tgtcaaatag ccaagtatct tgtaacacca ttccaaaact tttacgtaat gtatcacgtt
   100621 ggatagagcg gatgtcatgg tcatcgactg taattgttcc ttttgttgtg tcataaaaac
   100681 gcatcagcaa attaacaagt gtggattttc cggcccctgt ttttcctact atagcaatgg
   100741 tttgacctgg ataagcagta aaattgaaat cttcgatcaa acgctgcttg gggtcataag
   100801 aaaaagaaac atgatcaaaa gagacttttc ctttgatatt tttgggttcc aaggcatagg
   100861 catctggcaa atcaggcttt tcttctggct gatcgatcaa atcaaatgct cgttctagac
   100921 cagaaaaagc ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa
   100981 actgacttga gtagatcgta aagctggaaa tcacaccaac agtgattgac gaaccactct
   101041 tcaatgccaa taggccgcca actaaaccaa tcgataaata agcaaggtga tctacaaaac
   101101 gagaagacgg gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt tcatatagat
   101161 cagcatttag cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa gctttcacga
   101221 ttttctggtt accgattcgt tcggtcacaa agcccgagat atccccaata atcctttgct
   101281 gtctatcgaa atttgtttgt gaagcacgag caacgatcca actcacaagg aaaataattg
   101341 gtgtagaaca aagaacgacc aatgtcagaa gtggacttag acgcatcata aaaataaacg
   101401 aaagaatgat gattgccact ccagaaaata attgattgaa ggccgcagaa attgcaatgg
   101461 aaatattgtc catatcattt gtaaatcgac tgacgatatt tccgtgggag ttttgatcat
   101521 aataacgaag tggcaatcga ttcagatgag caaatgcatc ttttcttaat tttgcaacgg
   101581 ataagtaagc taaccgattg cttaacactt ggatgagcca ttgactgact acgttgatta
   101641 aaacgatacc accgaataga ataagtaaat ggaacagtcg attgaagcgg acctgtcctt
   101701 ctccaatcat ttgatcgata ctttgaccaa tttgtaacgt catcacaact gttgcaatcc
   101761 cattgatgat tcctagtatc acagctagcc aaatgtcttt tgcataggca gtaaggtaag
   101821 gaatgaatcg gcggagcgtc tgcaaggata atttttttac tttcataact attcttcctc
   101881 ctgtgatgcg acgatttcct gatattctgc tgattgagca agtaattctt catgggtacc
   101941 ctgagcagca atcttacctt gatccatcac taagatatga tgtgcttctt gaatagagcg
   102001 gacacgttgg gagataataa tcactgtact gccaagataa gtctgtagcg ccttacgcaa
   102061 atccaagtct gtttgataat ccaacgcact taaagagtca tctaaaatca gtaatgctgg
   102121 cttggcaatc aaagctcttg caatcgtcag tcgctgtttt tgtccgccag agaaattttt
   102181 cccattttcc atgacttttg tatctaaacc atttggaagc tgacggacaa attcttctgc
   102241 ctgagcgatt ttcagagctt cccaacattc tttttctgtg gcgtctttct taccccattg
   102301 aaggttttca cgaatagtcc cggtaaatag tacagaagtt tggggaacaa gagcaatttg
   102361 ctgacggaga tgatccagct gccaagaacg aacatccatt ccgtccatta taactgtacc
   102421 ggaactgaca tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt
   102481 tggaccaatg attccaagga ctgtatttcg cttcagctga aatgtaatat tttggagaga
   102541 aagaccagaa tcaggagtat aacggaagtc aacatggtca aaagcgataa ttgcttcaga
   102601 agaaaaatcg ggagtagtac tgacctcagc ttcaagaatc gaattttctg tgtctagcac
   102661 ctctttcacc cgatttcctg aagcttcggc acgtgtgaag atcacgacga ggtttgaaac
   102721 aacgatcagt gccaaaagca tttgattcat ataattgatc aaagcaagaa cttgtccttg
   102781 ttgcaagcta ccaatattta ctttgatccc gccaactgtc agcaaacaga tgatcccgac
   102841 attcatgatc aaggttgtag caggtgataa tagggcagag atattggaaa cacgtaaata
   102901 attgttcgct agatcatttg tgcttttatc gaacttctct gtttcttttt ttgtctgcgc
   102961 aaatgcgcgg atgacacgaa caccgctaag attctgacta acgtgttcat tgagctgatc
   103021 tagtttttct tgcacttttt ggtagagagg aacggttttt ttgatgataa aaaataaaat
   103081 gattgagaaa ataggcaata atgctaagaa aaacagacct acttcccagt cgatcacaaa
   103141 agccatgacc actgacccga tacttaaaaa cggtgctctg atcaataaac gaatgaccat
   103201 tgctaaagct agctgaagct gattgatgtc gtttgtcatt cgggtgatca aggtatctgt
   103261 tccaaaatga ttcaactcgt tgtgagagaa agtattgatt tttttgatta gctgattacg
   103321 taactctgta ccaaatcctt gtgaagctac tgaagcatag tattggcaaa tgatagcaca
   103381 aatcagacca atcacagaca tagcgaccat ccataagccc atttcaatga tttttcctcg
   103441 gtcattcatt tttagtccat tatcgatcaa ggaagccatc attaaaggca ggacaagttc
   103501 gaagacagct tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac gatagtcttt
   103561 tgcataacgt aataactgaa tcatatgatt cctcctatta tttatttgtc cacaattgaa
   103621 aaaacaagta ctaccacatt gtagcttagg aaatttagaa tgaaaagaga acaagccttt
   103681 tcataaatga aagttttatt atgaagaagc tttagaaatt gaatagacta ttttttttaa
   103741 aaaaaagaaa atgagaaaac atgccagtct cttcattcat aggaacgtct attttactta
   103801 atttttctat aaatgaattc tgcataaaaa atatatcaag taaatgttta tagatgaaac
   103861 tctatttact tcttttttaa tttgttcttg caatcttttt tgctacgtaa tttttgtaaa
   103921 aaagtgggtt gagagaaatc taattgattt tcaaagaagg agtatgaaaa tgagaaaaat
   103981 atttattcgt ttattgtggg tactatctta gaggaataaa taatagtgga gaaaactatg
   104041 gtttggcgca gattctatca ggtttgatgg aacgaagaca acctcccgac atcaagtcaa
   104101 aagctacttg agattttgat ctgaaaataa aaaaagaaaa caaggtagga acatcaatgt
   104161 ttctaccttg ttttcgtaat caatgggaaa aaactaagag tggtgtacgt aatcaataaa
   104221 aaggatggtg acaacagtac ctctttactc tctgaaagca tcataactcg tttttcctca
   104281 ttatgccaat aaattgttgt tataccacat ataaggattt gtaatagcta aaaacagaca
   104341 ggaaagttga aatctgtgaa tcttattttg tatggaagga tttatagaac gcaaaaatca
   104401 agataaagat gaataaaaat cctgtttcgg ctatatattg cgtatctacg ccaataaaac
   104461 gagtttgttc tgtcatagcc atataaagca aaggcaaaaa tacaactaca gcgagataag
   104521 atccgatgat ttctcgaaaa gttttccagg aagtatgttt cctgttttta tatttatccg
   104581 atttcattga atagtcatag cctccttatt ctttcatatg ttgaaattcc agtataaaaa
   104641 ctggaaggaa ctgtacgttt tttagaataa aaattataag ggataagtag gctgctgtct
   104701 ataataagat atatttttca attctttatt aaagatttta ttgacaaaat gaatccgttt
   104761 tctatgatgg aggaggggtg aataagttgt tgaagaagga aattttatgt tacttagaga
   104821 accagacagc ctttttcgat ttagaacata tgaacgaggt ctttacagca caaaggttag
   104881 ctgatcattt tggggtaaaa agaaacacag tcagtcatta tttgaaccag ttaaatgatg
   104941 agcaactact agtcaaaatc aattctcgac cagtgattta tttccacaaa gaagcttttg
   105001 aaaaacaatt ttttaagtta cgcacgaatt tttatttttc tatcgatgat ctcaaagagg
   105061 aacagccttt tttcgcgcaa ccaaaagact tattctcttt tatgatcgga tacgacgcca
   105121 gtttgaaaga aagtatcgaa caaatcaaaa cagctcttta ctatccagat ggcggattac
   105181 cacttttgat tacaggcgaa agtggcacag gtaaaagtta tttagttaat ttggtctatc
   105241 agtattgtct gcttcatgat ctgctagaag attctgcacc attcatcact tttaattgtg
   105301 cacaatatgc agataatcct gaacttttga ctagcaattt gtttggacat gttaagggag
   105361 cgtacactgg tgcagaagaa aataaaaaag gggcttttga agcagcagat ggtgggatct
   105421 tgtttttaga cgaggtacat cgcttaacac cagaaggtca ggaaaaactg ttcacctatc
   105481 ttgatcaagg tgtgatttat cgtatgggag aacctaattt gtcacgccgg atcaaaacac
   105541 gtctcttttt tgctacgact gaagagatta ctagtcattt cttaacaact ttcattcgaa
   105601 gaatacctat ccgaatcgaa ctacctgcac ttaatcagcg tagtcgtaat gaacggctag
   105661 aactgattta ctcttttttt ctcgaagaac aaagaaaaat ggggagccct ttgtcagtca
   105721 gtggaccagc tttgtcactt ttaacaagtc agaaattatc cggaaacata ggagagctga
   105781 aaaatatcgt caaagtttca gcagcaaaag catatgctga acagcgtgaa cagcctgaaa
   105841 tccatgtcac gatccatcat ttgcaaaaag aactacttgc gcagcctatt actaaaaata
   105901 cagcacagca aggaatctat attacgcaag accagacctt agagcaatta atggagaagc
   105961 aacagcccga acagcagcga atcgttaaaa gttttgagca aatcctaatt gatttcaaga
   106021 aaaataactg tctgctatat tcttgcgaag gaaaattaaa gcaagaagtg attcaattat
   106081 ttgatttttt gctgtttgag accagtagac aagaaaaaca cgagctgctg gtttattgga
   106141 cacaatctat ccgagagacc tttcgacaaa tggaaactgc ctatcaaatc aaattcgatg
   106201 gaaacagtgt ctatgccttg agttactatc tctatcagcg aagcactatc aaatggttac
   106261 cggaagaaca tgatattatc caactaatca aagaattaga acaacaagtt tctcagtctt
   106321 atccatctag ttatcactat gtccaacgaa ttctggaatt aagtaagatc aaattagata
   106381 ttgaagacac aagtatggat cggattttac tgacgatcta tctaaaaaaa gcgaaatggt
   106441 caaaagaaaa acgactgcca aaagcgatga ttgctgctca cggttatgca actgctagca
   106501 gtatggcaaa tgtagtaaat cgtttattac aagaggatct atttgaatca tttgatatgc
   106561 cactagatgt cacaccacag cagattgctt cagaaatcct tgattattgc gaaaacagcg
   106621 atgtttcaaa tggattgatc atattagtag acatgggatc tctgaaagaa atccatcagt
   106681 tttttaaaaa acagttatcc gtaccgcttt tgattttgaa taatgtgacg acacctttag
   106741 caatcacagt aggggaatgc ctgcaaaaaa atgtatcatt agaagagata gcagaagaga
   106801 ctgttcgaca gatccagcca gaatggcgac tgctctatcc agaggaaaac aaacccaaag
   106861 cactcattac tacttgcttc actggaatcg gtacggcggt ccacttaagt gaactgctag
   106921 aaaaaagctt acctacggct tgtcagttga aaatcatccc atatgaatat cagcaattaa
   106981 aagataagaa aaacagtgac ccattatttt ccatatacga agtcgtagga atgattggca
   107041 ccacagatcc tgatatcacc aatatcgact atctttcatt agaagacctc atctcggggg
   107101 aaaagatgtc tgtactagct gaatggttgg acactactat gaatgcttca gagaaggaaa
   107161 tattcaatca gcgcatcatt cgtaattttt cattggaaaa agtactggat tccgtgacta
   107221 ttctagatac agagaaggtg atgggagaaa tcgatcattt tatgagagaa ttagaggtgc
   107281 agttgaatcg aagcatcagc aatcccaaaa aattagcgtt gtatgtccat gtgagctgtc
   107341 tcattgaacg attgatccga caaatgccaa tcgaaaccta tcaaggatta gagaaatgga
   107401 agcagtgtca aaaagaaggg ttatctgcta ttaaatctgc gtttagtgtc attgaagagc
   107461 attatagtgt catgatcccc aattctgaga ttgcctatat ctatgatatt ttgagtgaag
   107521 atacagagtt tctctcgata gaagaagaat tttgaacata agcaagttgt tgccactaat
   107581 tttggcatgc aacttgcttt tttagtatgt gtaagtgaaa agaggagatg aaaaagtgga
   107641 tagagaaatt atcttagctt cccatggaaa atttgcttca gggatattag attcattaga
   107701 gctgatctat gggaaaaatc attctattac tgtattcgat tgctatactg atgagaattt
   107761 tgatttagcg gaaacagtaa ggcagctttt tatgaggtac aaagataaag aaatcattgt
   107821 tctgacggat ctttttggcg gaagtgtcaa caacgaattc ttacaatata tcaatcggga
   107881 tcatgtctat ctcgttgccg gattgaattt gccactattg attgagctag tatctcggtt
   107941 ggacgctgaa gtagagacaa ctgttttgat ccagcaggtg ctaactgatt caaaaaaaat
   108001 gatccagttt tgtaacgagc ggctcaatca gaaaatagaa gaagacgaat tttaggagga
   108061 agaaaaatga ttgtattgac aagagtcgac catcggttat tacacggaca agtagctttt
   108121 tcatggacac aaacaatagg cgcagactgt attttgattg ctaatgatga tgtacctacg
   108181 aatgagatac gaaagacaac gatcaaatta gccaagccac aaggagtcaa actagtgatc
   108241 aaatcaattg atgactcgat tgccgcgttg aaaagcgggg tgacagataa atataagtta
   108301 ttcatcgtag tggaatcgat cgaggatgca tataagctgg cagaagcgta tccagaaatc
   108361 aaaaccatca acttaggagg gatcaaggca aaacctggta ctcgcagcat cggaaaagcg
   108421 gtaaatattt taccagaaga ggaggaacgc ttgaaacgat taattgacaa acaaatagaa
   108481 gtagaaatcc gtcaagtacc agcagataaa aaagtggatg taagagaagt tttgtaagaa
   108541 aggaagaacc tcaatgattg tacaagcagt cttattagga attgttgcat ttgtcgcaca
   108601 atcagaatat gcactaggga catcgttgct ttctcgtccg attgtgacgg ggctgtttac
   108661 aggaatcgtg ttaggtgatg taaaaactgg gatcattatg ggcgcaactt tagagttagc
   108721 atttatcgga tcgttctcag taggtgcttc gattccacct gatgttgtga ccggtgggat
   108781 tttagggacg gcttttgcta ttacagctgg agccggtaca gaaaccgcgc ttttactagg
   108841 tttgccgatt gctacgttga cattgatatt gaaaaatatt tacttagggc ttttgattcc
   108901 aattatgaac cataaagcag acaattatgc agaagaagga aattataaag gagtcgagca
   108961 tatgcacttg cttgccggta tcggtctttc cttgatgctt ggtgttgtgg taatggtttc
   109021 ttatatggtg ggaagcaata cgatcggaaa tctattaaat atgattcctg acttcgttca
   109081 aaaggggttg tcagtagcta cagggttgat tccagctctt ggttttgcga tgcttgctcg
   109141 tttgctgatc aacaaacaag ttgcaccata ttttttctta ggttttgcta tagcttctta
   109201 cttagagatt cctgtgaccg gagtagctat ttttggtgcg attttagcag ttgtggtagt
   109261 caatattatg aatgtccgtc aactaccact tcaaacaact gcaggggagg tagaagaaga
   109321 tgaagaattc tgagatcaaa aacagcaaaa taacgaaaaa agagctaaat cacgtatttt
   109381 ggcgttcttt tcaaatggag ttttcttgga attatgaacg tcagatgaat atggcttatg
   109441 tttatgcaat gattccgatt ttgaaaaagt tatatcagtc gaaagaagaa atggcagcag
   109501 ctttgaaacg gcatctagag ttttttaaca cgaccccaca tatcgttaca ctgattttag
   109561 gaatcacggc tgcaatggag gaagaaaata aggatgatcc tgaatttgat gtgactgcga
   109621 tcgatagtat caaaacatct ttgatgggac ctcttgcagg gttaggagat tctttctttt
   109681 ggggaacttt gcgattgatt gccacaggtg tgggtacctc tttagccatg caaggaaata
   109741 ttttaggccc tatcttgttc attttgattt tcaatattcc gcacattctg tttcgctatc
   109801 tagcaacagg ttggggatat cgcttaggaa cgggtttttt gaaaaagatc caggcaaacg
   109861 gtatgatgga aagtttgaca ttaggcgcgt ctattattgg actgatggtc gttggagcaa
   109921 tgactgccac gatgatcacc atcaatattc ctttaaaaat cggttcagga gaaaatgctg
   109981 tgactgttca aagtattttt gatgatatcg ttccaaatat cttatgtctt ggagcatttg
   110041 gcatagtgtt ttatctattg aagaaagaag tgaaaccatt aacgatcttg atcggactag
   110101 ctttctttgg aatttttggt tcgtggatag ggatttttta aaaaaggagc agagaaaatg
   110161 tttacgatgc aagattatat ctatgaagaa aaggaagtac taagtacgat cttgaaaaag
   110221 aatgattttt cgacaagaga acatatgaaa aaaacagtga atttactgat cttagcaacg
   110281 ggttcatctt ataacgcttg tttagcagcg aagccagcat tggaatctta tggtgatctt
   110341 actgtcgata ttcaagaacc tttttatttt ttcaattatg gaaaactttc tccttcaatc
   110401 gatactgtga ttgctgtgtc acaaagcggg aaaagtgctt ctactgttga agcagtaaaa
   110461 atgatacaaa aacaagggtt acctgttgtt gctatcacaa atgacgtgca aagtccactt
   110521 gctttagaag ctgcacaaat tattgattta ggtgccgggg tagaatcagt aggcttcgtt
   110581 acaaaaggct attctgcgac tgttttacag ttactactct tagggatagg aattggaata
   110641 agtaaaaata aaatcagcaa aaaaatagaa caagactaca tgcaacagtt gcgcaaaata
   110701 atcaaccatt taccagcgat tattcaaaaa acggaagaat tttttgatgg atatcaaagt
   110761 ttatttcgtt tagcacagcg tttcgtcacc attggatatg gtccaaactg gggaacagct
   110821 aaagaagcag agacgaaatt gactgaaaca atccgcgttc cgtcacaagg atttgagtta
   110881 gaagcttata tgcacggccc atacctggag gcagatgctt cgcatttgct attctttatt
   110941 gaaggggaaa gtgtaaataa ggaacgttcg caaaagttgc aaagatatat gagtcggtac
   111001 gttggagaga ctctcaccat taccacaaaa aaagcaagaa acgagaaaac acttggatta
   111061 gcaatagaat gtgatgaata tctgtctgta ttagcgcttg tcgttccgtt tcaactattc
   111121 gcttataaaa ccgcagtagc taaaggtatt gacttgaaca aaaagatttt tgaagacttt
   111181 gatacagtct tgaaaagtaa attataagag gtggaaatca tgctgaaatt caatgaagaa
   111241 gaacaaatca aagcaataaa aggcgcacta gctttacgtc cgcaagtaga agaaatcatc
   111301 gataaacttt atgcggaaaa atttgacgct gtctattatc taggaatcgg tggaacgtat
   111361 gcttcctcga tgcaagctgt cacttatatg aatgggaaaa gcaacttgcc tgtctttgta
   111421 cagcatgctg cagaatatta cactactgga aacaaaagat taacaaaaga ttctatcgtt
   111481 attttgtcct ctgttaccgg tacaactcaa gaagtagtga aagcagtaga acaaatcaag
   111541 aaagtaggag cgacactgat tggatttatc gataaagcga acagtaagtt gtctcagcta
   111601 tgcgattttg tggtcactta tccagctcct ggaacagaac aaatcaaatt ttttatggca
   111661 gcagatcgat tgatgtattt gaatggagaa ttcgaagctt attcagaata ctatgaacag
   111721 ctcgaacgtt atttaccaac agggttagtg gaagcagaga aaaaagcaga tgcatttggc
   111781 ttatcatttg cagaaaaaca tcgacgtgat agtatgcatt attttattgg agcagggaat
   111841 caatggggag ctgtttattc atatgccatg tgttactggg aagaacaaag ctggcttcca
   111901 tcgaaatcta tccatgctgc cgagtttctt cacgggacgt tagagatcgt agaagaaaca
   111961 accccagtca cattattttt aggcgaagat gaacagagag tgttatctga acgtgtggct
   112021 aaacttttgc caaaaatttg cagcaattat acattgatag acacaaaaga ctatccagta
   112081 gaaggtatca gtgaaaaata tcgcggacgt gttttgtctt tcttattgat gcatgtagtg
   112141 acacaacgca tcgatgctca tgtggagcag ttgaactgcc atccattaga gatacgccgc
   112201 tactatcgtc agtttgatta ttaggataga ctggataaaa agaatataaa aaataaagag
   112261 agcagcgata actgctccct ttattttttt aaaatattgg catcaattac gttataaaac
   112321 acattttttg cattatctac agttcaactt ataatctgtt taatgggaca tgtgggtgcc
   112381 aaccattttt tgttatgcaa tagtactatt tagttgttgc atggatccgt agtaaaagtc
   112441 caagtacaac gagttgcgcc agtttacata gcgttttgta tccagaatgt tgactaaaac
   112501 aaaagttcct atgtcaaccg tcaacagaga caatctgtcc gttgcaggac ctgacagatt
   112561 tagaaatgtt caatctgtct cttatgtatt ttaaatcaat actcactagg agaagtttcc
   112621 taacctccta atagtaatgc tggtagtaac attgtcaaaa ctgcttcctc ttaggaacaa
   112681 gcaaagcata acgagtgtaa aaataaaatg tagactattg taacatatca ttgattattt
   112741 caagtaaaaa cgataaaaga agtaaacaaa cacttaaaaa caatggaaat tagtcccaac
   112801 tttaagaaat gtatactata atggaaatta tcaattattt atttggagga atatccatga
   112861 aacaaaaaat cgcagttctt ggtcctggat cttggggcac tgctttagca caaacattgg
   112921 cagaaaacgg gcatgatgtc cgtatttggg gaaatgtacc tgaacagatt gatgaaatca
   112981 ataagtatca tacaaatcag cattttttac ctgatttgac gatcccagaa tcgattatcg
   113041 gatataaaga attggcagaa gcagtggata acgcagatgc tattttattt gttgttccaa
   113101 caaaagcgat tcgttcagtc gcacaggaac ttattggtaa aatgaatact aagccaatca
   113161 ttatacatgc aagtaaagga ctagaacaag atacgcataa acgaatctca gaagttttgg
   113221 ctgaagaaat tccagaagaa aaaagacagg caattgttgt attatctggt cctagccatg
   113281 ctgaggaagt ggcagtacat gatatcacaa caattacagc tgcaagtatt gatcaaaaag
   113341 cagcagccta tgtacaaaaa ctgttcatga atgattattt caggatttat acaaataatg
   113401 atgtgattgg cgtagaaact ggtgcagcac tgaaaaatat cattgcaatt ggtgccggcg
   113461 ctatccatgg attaggattc ggagatgatg caaaagcagc aatcatgact cgaggattgg
   113521 cagaaatcag ccgtcttggt gtcgcgatgg gtgccaatcc tctgacattt atcggtttga
   113581 gtggtgtggg ggacttgatc gttacttgta caagtgttca ttctcgcaat tggcgtgccg
   113641 gtaacttgtt aggacaggga cataagctgg aagaagttct agaaaacatg ggaatggtcg
   113701 ttgagggtgt ggcaacgaca aaagcagcag tagagctagc tcaacagcta ggtgttgaga
   113761 tgccaattac tcaaacaatc tataatgttt tgtataatgg agaagatatc aaaaaagcag
   113821 caaaagaaat catgttacgt gacggaaaaa tggagaatga attttctctt cgttaaatag
   113881 atgatcaata gttaatcgt
//
LOCUS       DACCCS010000002        84730 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.002, whole genome shotgun sequence.
ACCESSION   DACCCS010000002 DACCCS010000000
VERSION     DACCCS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.002"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            83..970
                     /locus_tag="IVK82_000104"
     CDS             83..970
                     /locus_tag="IVK82_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6391201.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IVK82_000105"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IVK82_000105"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP6391202.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IVK82_000106"
     CDS             2272..2718
                     /locus_tag="IVK82_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP6391203.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IVK82_000107"
     CDS             2991..4343
                     /locus_tag="IVK82_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP6391204.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IVK82_000108"
     CDS             4514..4801
                     /locus_tag="IVK82_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391205.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="IVK82_000109"
     CDS             5027..5470
                     /locus_tag="IVK82_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6391206.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="IVK82_000110"
     CDS             complement(5476..6660)
                     /locus_tag="IVK82_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP6391207.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="IVK82_000111"
     CDS             6928..7626
                     /locus_tag="IVK82_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP6391208.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="IVK82_000112"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="IVK82_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP6391209.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="IVK82_000113"
     CDS             complement(8436..8969)
                     /locus_tag="IVK82_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391210.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="IVK82_000114"
     CDS             complement(9266..11647)
                     /locus_tag="IVK82_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP6391211.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IVK82_000115"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IVK82_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP6391212.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12887
                     /locus_tag="IVK82_000116"
     CDS             12348..12887
                     /locus_tag="IVK82_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP6391213.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12977..13396
                     /gene="gpsB"
                     /locus_tag="IVK82_000117"
     CDS             12977..13396
                     /gene="gpsB"
                     /locus_tag="IVK82_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP6391214.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13418..13785
                     /gene="rnpB"
                     /locus_tag="IVK82_000118"
     ncRNA           13418..13785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVK82_000118"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13994..15160
                     /locus_tag="IVK82_000119"
     CDS             13994..15160
                     /locus_tag="IVK82_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP6391215.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15206..16702
                     /locus_tag="IVK82_000120"
     CDS             15206..16702
                     /locus_tag="IVK82_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP6391216.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16783..17466
                     /locus_tag="IVK82_000121"
     CDS             16783..17466
                     /locus_tag="IVK82_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6391217.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18111..19301
                     /locus_tag="IVK82_000122"
     CDS             18111..19301
                     /locus_tag="IVK82_000122"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP6391218.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19403..20356
                     /locus_tag="IVK82_000123"
     CDS             19403..20356
                     /locus_tag="IVK82_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP6391219.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20425..21774
                     /locus_tag="IVK82_000124"
     CDS             20425..21774
                     /locus_tag="IVK82_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6391220.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21957..22280)
                     /locus_tag="IVK82_000125"
     CDS             complement(21957..22280)
                     /locus_tag="IVK82_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391221.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22409..23572
                     /locus_tag="IVK82_000126"
     CDS             22409..23572
                     /locus_tag="IVK82_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391222.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23642..24991
                     /locus_tag="IVK82_000127"
     CDS             23642..24991
                     /locus_tag="IVK82_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP6391223.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25036..25467)
                     /locus_tag="IVK82_000128"
     CDS             complement(25036..25467)
                     /locus_tag="IVK82_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6391224.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25855..28497
                     /gene="alaS"
                     /locus_tag="IVK82_000129"
     CDS             25855..28497
                     /gene="alaS"
                     /locus_tag="IVK82_000129"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP6391225.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28697..29293
                     /locus_tag="IVK82_000130"
     CDS             28697..29293
                     /locus_tag="IVK82_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6391226.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29415..30563
                     /locus_tag="IVK82_000131"
     CDS             29415..30563
                     /locus_tag="IVK82_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP6391227.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30601..31014)
                     /locus_tag="IVK82_000132"
     CDS             complement(30601..31014)
                     /locus_tag="IVK82_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP6391228.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31093..31524
                     /locus_tag="IVK82_000133"
     CDS             31093..31524
                     /locus_tag="IVK82_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP6391229.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31623..31856)
                     /locus_tag="IVK82_000134"
     CDS             complement(31623..31856)
                     /locus_tag="IVK82_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP6391230.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32039..33709
                     /locus_tag="IVK82_000135"
     CDS             32039..33709
                     /locus_tag="IVK82_000135"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP6391231.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33856..34242
                     /locus_tag="IVK82_000136"
     CDS             33856..34242
                     /locus_tag="IVK82_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391232.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34229..34927
                     /locus_tag="IVK82_000137"
     CDS             34229..34927
                     /locus_tag="IVK82_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6391233.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34932..35396
                     /locus_tag="IVK82_000138"
     CDS             34932..35396
                     /locus_tag="IVK82_000138"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP6391234.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35393..36304
                     /locus_tag="IVK82_000139"
     CDS             35393..36304
                     /locus_tag="IVK82_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP6391235.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36539..37078
                     /gene="pyrR"
                     /locus_tag="IVK82_000140"
     CDS             36539..37078
                     /gene="pyrR"
                     /locus_tag="IVK82_000140"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP6391236.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37092..38375
                     /locus_tag="IVK82_000141"
     CDS             37092..38375
                     /locus_tag="IVK82_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP6391237.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38388..39314
                     /locus_tag="IVK82_000142"
     CDS             38388..39314
                     /locus_tag="IVK82_000142"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP6391238.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39333..40613
                     /locus_tag="IVK82_000143"
     CDS             39333..40613
                     /locus_tag="IVK82_000143"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP6391239.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40618..41694
                     /gene="carA"
                     /locus_tag="IVK82_000144"
     CDS             40618..41694
                     /gene="carA"
                     /locus_tag="IVK82_000144"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP6391240.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41699..44884
                     /gene="carB"
                     /locus_tag="IVK82_000145"
     CDS             41699..44884
                     /gene="carB"
                     /locus_tag="IVK82_000145"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP6391241.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44938..45714
                     /locus_tag="IVK82_000146"
     CDS             44938..45714
                     /locus_tag="IVK82_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP6391242.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45711..46643
                     /locus_tag="IVK82_000147"
     CDS             45711..46643
                     /locus_tag="IVK82_000147"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP6391243.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46640..47356
                     /gene="pyrF"
                     /locus_tag="IVK82_000148"
     CDS             46640..47356
                     /gene="pyrF"
                     /locus_tag="IVK82_000148"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP6391244.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47363..47992
                     /locus_tag="IVK82_000149"
     CDS             47363..47992
                     /locus_tag="IVK82_000149"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP6391245.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47980..48294
                     /locus_tag="IVK82_000150"
     CDS             47980..48294
                     /locus_tag="IVK82_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391246.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48332..48973
                     /locus_tag="IVK82_000151"
     CDS             48332..48973
                     /locus_tag="IVK82_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6391247.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49004..49708
                     /locus_tag="IVK82_000152"
     CDS             49004..49708
                     /locus_tag="IVK82_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP6391248.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            49705..50607
                     /locus_tag="IVK82_000153"
     CDS             49705..50607
                     /locus_tag="IVK82_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6391249.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50765..52471)
                     /locus_tag="IVK82_000154"
     CDS             complement(50765..52471)
                     /locus_tag="IVK82_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP6391250.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52661..53284
                     /locus_tag="IVK82_000155"
     CDS             52661..53284
                     /locus_tag="IVK82_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391251.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53734..54783
                     /locus_tag="IVK82_000156"
     CDS             53734..54783
                     /locus_tag="IVK82_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6391252.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54780..55661
                     /locus_tag="IVK82_000157"
     CDS             54780..55661
                     /locus_tag="IVK82_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6391253.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55664..56434
                     /locus_tag="IVK82_000158"
     CDS             55664..56434
                     /locus_tag="IVK82_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6391254.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56713..57567
                     /gene="aroE"
                     /locus_tag="IVK82_000159"
     CDS             56713..57567
                     /gene="aroE"
                     /locus_tag="IVK82_000159"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP6391255.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57745..58908
                     /locus_tag="IVK82_000160"
     CDS             57745..58908
                     /locus_tag="IVK82_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP6391256.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59025..59435
                     /locus_tag="IVK82_000161"
     CDS             59025..59435
                     /locus_tag="IVK82_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP6391257.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59609..61837
                     /gene="topB"
                     /locus_tag="IVK82_000162"
     CDS             59609..61837
                     /gene="topB"
                     /locus_tag="IVK82_000162"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6391258.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            61975..62292
                     /locus_tag="IVK82_000163"
     CDS             61975..62292
                     /locus_tag="IVK82_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391259.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            62452..62688
                     /locus_tag="IVK82_000164"
     CDS             62452..62688
                     /locus_tag="IVK82_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6391260.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62685..63083
                     /locus_tag="IVK82_000165"
     CDS             62685..63083
                     /locus_tag="IVK82_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP6391261.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(63750..64694)
                     /locus_tag="IVK82_000166"
     CDS             complement(63750..64694)
                     /locus_tag="IVK82_000166"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP6391262.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(64990..65934)
                     /locus_tag="IVK82_000167"
     CDS             complement(64990..65934)
                     /locus_tag="IVK82_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6391263.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            66155..67369
                     /locus_tag="IVK82_000168"
     CDS             66155..67369
                     /locus_tag="IVK82_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6391264.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            67391..69406
                     /locus_tag="IVK82_000169"
     CDS             67391..69406
                     /locus_tag="IVK82_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP6391265.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(69489..70862)
                     /locus_tag="IVK82_000170"
     CDS             complement(69489..70862)
                     /locus_tag="IVK82_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP6391266.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(70888..71172)
                     /locus_tag="IVK82_000171"
     CDS             complement(70888..71172)
                     /locus_tag="IVK82_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6391267.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(71174..73189)
                     /locus_tag="IVK82_000172"
     CDS             complement(71174..73189)
                     /locus_tag="IVK82_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6391268.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(73368..73673)
                     /locus_tag="IVK82_000173"
     CDS             complement(73368..73673)
                     /locus_tag="IVK82_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP6391269.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(73762..74793)
                     /locus_tag="IVK82_000174"
     CDS             complement(73762..74793)
                     /locus_tag="IVK82_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP6391270.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            75000..75875
                     /locus_tag="IVK82_000175"
     CDS             75000..75875
                     /locus_tag="IVK82_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6391271.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(76027..76869)
                     /locus_tag="IVK82_000176"
     CDS             complement(76027..76869)
                     /locus_tag="IVK82_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6391272.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(76961..78838)
                     /locus_tag="IVK82_000177"
     CDS             complement(76961..78838)
                     /locus_tag="IVK82_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6391273.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(78835..80256)
                     /locus_tag="IVK82_000178"
     CDS             complement(78835..80256)
                     /locus_tag="IVK82_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6391274.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(80259..83648)
                     /locus_tag="IVK82_000179"
     CDS             complement(80259..83648)
                     /locus_tag="IVK82_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP6391275.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            83905..>84730
                     /locus_tag="IVK82_000180"
     CDS             83905..>84730
                     /locus_tag="IVK82_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6391276.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKN"
BASE COUNT        27129 a        14958 c        17481 g        25162 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaat cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
    12661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
    12721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
    12781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
    12841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
    12901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
    12961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
    13021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
    13081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
    13141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
    13201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
    13261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
    13321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
    13381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
    13441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
    13501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
    13561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
    13621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
    13681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
    13741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
    13801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
    13861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
    13921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
    13981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
    14041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
    14101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
    14161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
    14221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
    14281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
    14341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
    14401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
    14461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
    14521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
    14581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
    14641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
    14701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
    14761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
    14821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
    14881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
    14941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
    15001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
    15061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
    15121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
    15181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
    15241 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
    15301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
    15361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
    15421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
    15481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
    15541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
    15601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
    15661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
    15721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
    15781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
    15841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
    15901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
    15961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
    16021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
    16081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
    16141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
    16201 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
    16261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
    16321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
    16381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
    16441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
    16501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
    16561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
    16621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
    16681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
    16741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
    16801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
    16861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
    16921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
    16981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
    17041 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
    17101 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
    17161 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
    17221 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
    17281 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
    17341 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
    17401 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
    17461 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
    17521 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
    17581 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
    17641 cggaattggc aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc
    17701 acagtccttt tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt
    17761 aaaaattgct gataaacgaa gatggaaatt ttatgtattt ttattggtct atgcaataaa
    17821 taaattaaat attcagaata caattgattt taacgaaagg gcttctatca atggttgtgt
    17881 tgtatttatc tgcccaggta gagtaaaata gatcattatc taaaaggtaa ttgttcatac
    17941 tatataagtc atttgaaagt aaaaagatac tgtaaataaa aaatgtggtt aaataattct
    18001 cataatcagt gtaagcgtta tcaaaaatat agcatacttg ctttgtagat gaatgacacg
    18061 atatatgttt ggtatgttct gcaagacaga tgaatatgga ggtggagaca atgtcttcca
    18121 aagaactaac aatgtattta gagaaagaat tggatcaact gaaagaaaag gggttataca
    18181 atacgatcga catactagaa agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga
    18241 tgatcaatct agcatccaac aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag
    18301 catgtattga agcaaccgaa acttatggtg taggagcagg agcagtccga acgatcaatg
    18361 gttcgctgaa aatccatcag caattagaag aaaaaatcgc agaattcaaa gggacagaag
    18421 cagcaatcgc ctttcaatct ggattcaact gcaatatggg cgctatctct gcagtaatga
    18481 caaaagaaga tgctatctta tcggatgaat taaaccatgc atctattatt gacggttgcc
    18541 gcttatccgg agcaaaaatc atccgaatta aacatcaaga tatgaaagac cttgagaaaa
    18601 aagcaaagga agcgatagaa agtaagaaat ataaaaaaat catgtatatc acagatggtg
    18661 ttttctccat ggatggagac attgcacgat tgccggaaat tattccgatt gttgaaaaat
    18721 atggactgat cacctacgtc gatgatgcgc acggctcagg cgtaactgga aaaggtgcag
    18781 gaaccgtcaa gcattttgga ttaagcgaca aaatcgatat gcaaatgggc acgttatcga
    18841 aagctatagg tgttgttggg ggttatgtag ctggatcgaa aacactaatc gactggttga
    18901 aagctagaag tcggccattc ctattttcta cttcgctaac tccaggtgca gctgcagctg
    18961 cattggcttc gattactttg atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa
    19021 atgctaatta ttttaaagaa gaattgaaaa aagtaggcta taacatcggt atgtctgaaa
    19081 cacctattac tccagtcatt ttgggagacg aaaaagtgac tcagactttc tctaagaagc
    19141 tgatcgaaca tgggatttat gctaaaccaa tcgtttatcc aactgtgcct cttgggacag
    19201 gcaggatcag aaatatgccg actgcggaac atacgaggga aatgcttgat gaagcagtag
    19261 cagtttatca aaaaatagga aaagaaatgg aaattattta actaaattta gttttttcgt
    19321 ctatttaaaa ttagagaaca tgttcacgca tatattgtta atcatctgaa gatagtagta
    19381 aaaaagatca gggaggatca tcatgaaaaa agtattggtg acaggatgtt taggacaaat
    19441 tggttcagag ttgatcacac ggctacgtca agaactaggc agtgaaaatg tcattgcgac
    19501 agatatacgc atgcctgaag tcattgagaa aaacgatatc tttgagatcc tagatgtaat
    19561 ggattacgaa aaaatgcgca gcatagcaga acaatatcaa gtggacacat tgattcattt
    19621 ggctgctctt ttgtcagctg ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt
    19681 gactgggctg gtcaatgcat tggaagtcgc tagagaattc cagctgaaat tttttacgcc
    19741 aagttcaatc ggcgcgtttg gtccaaatac tccaaaagat cataccccac aagatactct
    19801 tcagcgtcct acaaccatgt atggggtaac gaaggtagct ggagaactac tatgtgatta
    19861 ttattatgtg aaatatggcg tagatactcg tggtgtccgt ttccctggat tgatttctta
    19921 tgcaaccttg ccaggaggcg gtacgacaga ctacgcagtc gatatttatt attcagcaat
    19981 caaagaagga aagtacacta gtttcattga tcaaggtaca tcaatggata tgatgtatat
    20041 gccagatgcg atcgatgcca ttataaaact catgaatgca gatcctgatc gattggttca
    20101 tcgaaatgcc ttcaatatat cggcgatgtc ttttgacccg gaagagatca aagcatctat
    20161 tcaaaaaatc ataccagatt ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat
    20221 agcagaatct tggccaaatt cactagatgt ttcttgtgcg caagaggaat ggggcttttc
    20281 gccgaaatac gacttagatg tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt
    20341 ggtagagacg gtataaaaca agaaccacat ccaaaaagtt ttttatactt ttgggattgg
    20401 ctatggtatg aaaggggaca agggatggaa gaatatcaag ccacgccagt aaaagaagta
    20461 aaaatagaaa atgaattaaa acgtacgatg ggatttttta ctgctttatc gactgttatg
    20521 ggcacagtga tcggtgcagg ggtatttttt aaagccgcaa gcgtagcaga agttacagga
    20581 tccgccagtt tgcatatgtt ttcttggttt ctcggtggta tgatttctgt ctgtgcagga
    20641 ctaacaggag ctgaactggc agctgctatt ccagaaacag gagggatgat taaatatatc
    20701 gaacggattt acggaaatac agcagctttt ttattgggct gggcgcaagt cgtgatttat
    20761 tttccagcaa atgtagcagc cttgtcgatc attttcggta cacagttcgt caatctattt
    20821 ggtctttccc agtcaatgat tgttcccgta gctgttacag ctgctgtatc gatcctgttg
    20881 atcaattttt taggttctaa agctggaggg gcttttcagt cgattacgct tgtctgcaag
    20941 cttattcctt tgtttgtgat cgtcattttc ggtttgttcc gacaagaagg agtagacttc
    21001 caactgtttc cgatccaagc aggagaaaat ctttcatttt tctcagcctt gggagcgggc
    21061 ttgctagcaa cgatgttcgc atacgacggt tggatacacg taggaaatat ttcgggagaa
    21121 ttgaaaaagc cggcgaaaga tttgccaaaa gcaatttctt taggaatcat cggaatcatg
    21181 atcgtttatt tattagtaaa tgcggtgttc ttaagaaccg catctatcga cggagtcgca
    21241 ggaaacagca atgcggcaag tgacgttgca aaaatgattt ttggcggctt cggtggaaga
    21301 ctggtcacag ttggtatctt gatttctgtt tatggaacca tcaatggcta tacccttact
    21361 ggaatgcgtc tcccttatgt tatggcaaaa gaaaacaacc tgccttttag caagctcttt
    21421 gctaaacttc atgataaaac taaagttcct gtagccgcag gtatcttaga gctagtcatc
    21481 gctattggca tgatgatgat cggtggtttt gatacactaa cggatatgct gatctttgtc
    21541 atttggattt tttatacgat ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct
    21601 gatttgtttc gaccatacaa agttccaatg tatccgttta ttccactagt agcaatcatt
    21661 ggagggactt ttatcgtatc aagcacgctt atcactcaga cctttttagc ttcgatgggg
    21721 atagtattga cacttgctgg tatcccgatt tatctctatt taaaacgtag atagctaaaa
    21781 cattcgaatt ttattagaac tgcacttctc tgccaaacaa aatattttat ttaaatgagg
    21841 agtacgtgga agaaatgtca aaagtactcg ttaattttga tcacgtaaat gtcgttttgt
    21901 ctacgatgaa atagcaaagt aaaagacaga aatcgggctc aaccgatttc tgtcttttac
    21961 tttgatttgc gtaactgaac tattcggcgt acgccgcgat aaatatttaa taaaaccaag
    22021 caaccagaaa tgaaaatggg aagccaaatc agatagacag ggtagccaag cactttagac
    22081 aaagcatgtg gagtcatttt atccatttca agaagtgctt gctgccaagt ctgaagattg
    22141 aagactagca tgaaacccga aataatcagg agaataaatc ctaaataatg aattgtagtt
    22201 ctttttctta cataagcgat cgtagcataa aggaacagta aaaaaatagg tacgccaaaa
    22261 acaaagtaca tattcagcat ctcgacaccc cctttgtgtt tatcttactt tactttgaaa
    22321 gaaatgagaa ataatagcct cgttacttgc ttgaaacagc aaaggactgc taagataaga
    22381 gagatacaaa gaatcaagga ggagtcatat ggcaagttat atcgaaccga ttcgttttgc
    22441 gctgattgtt tttccatttt tagccttagc gatctcatct gtttttttta tttatgaata
    22501 tcgaaagtat ggaacatttc tagtaacaag ggcagttatt ttgtattctt tcgtttttta
    22561 tttattgtgt gcgtattttt tggtgatctt accacttccg gcaaaggaga cggttgccca
    22621 aatgacaggg ccaaagatgg agctgcattt atttgcttca tggcaacatt tcagagacaa
    22681 cacggtattg gtcttgtctg aacctagtac ctacctgccg gcaatgaagc agagtgtatt
    22741 tttggaacca gcttttaatg ttttattact cataccattt ggcatttatt taagatatta
    22801 tttccggctt tcttttatga aaacgatgat tgctagtttc tgcctttctc tattttttga
    22861 attgacgcag ctaagtggac tttattttat ctacccaaga ccataccgct tatttgatgt
    22921 gaatgattta gtgacaaata cattgggtgg aatcattggt tttgtcgtta cgccgttatt
    22981 tacatttatg cttcctacaa gaagacgaat ggatgaactt tcttatgaaa aaggaaagac
    23041 tgtctcattg actcgacgct tggtcgctta tttgatcgac tggacatttt tatctcttat
    23101 taatttggta gcggcgattt tctttaaaat tgcgttcgat tcaggggcag ttacttctca
    23161 tatctggtgg cttttattag aagtaggggt atactttatg attgtaccgt ttataaccaa
    23221 tggatggaca cttggcaaaa aaattgtgcg tatccgtttg gtggaagaag accgcgaacg
    23281 aatttcttta aaagcattga tgatccgcta cgggtatctg tactttcttt tttatggttt
    23341 aagctggttg gggcagttgc ttcagcaaat catgaattcg acaacgaata cggtgttgca
    23401 gatgagtgca gtattcattt ctctcggtgt cttgttacta caaggtattt ttattttaaa
    23461 tatcttgcta tcatttttga gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac
    23521 atatactgtt agcacgattg gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa
    23581 aaacgctttg tctgtgttat aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg
    23641 aatgaataat tttgatatat tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa
    23701 gggcttcaaa cagcccacag aggtccagga acgtttgatt cctttaattg caaaaggaaa
    23761 aaatatcatt gggcaatctc aaactggtac aggaaaaaca catacgtttt tgttgccttt
    23821 gtttgaaaaa atcaatgcag aaaaagagga agtacaagtt gttattacag ctccaagtag
    23881 ggaactagct gcacagatct atgaagcagc tacacaaatc gctaaatttt ctacaccgga
    23941 gatccgtgtc tcgaattttg tcggcggtac agacaaacag cgtcaattaa atcgtctgca
    24001 tcaccaacag ccacaggttg tagtaggcac gcctggtaga atattagaca tgatgaatga
    24061 acaagcgctg aagattcaca ccgcatttgc atttgttgtc gatgaagcgg atatgacatt
    24121 ggatatgggt tttttaaatg aagtagacca aattgcaagt cgattaccag aaaagctgca
    24181 gtttttagtt ttttctgcca ctattccaga aaaacttcgt ccattcttac gaaaatacat
    24241 ggaaaatcca atcattgaag aaatcaaacc aaaagcagtt atttctgaag acattgaaaa
    24301 ttggctgatt tcaacaaaag gtaaagatga gaatcaaatg atttatcaac tgctgacaat
    24361 tggtcatcct tatttagcaa tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc
    24421 cgattacttg aaaaaccaag gcttgaaagt agcaaagatt catggagaca tcaccccaag
    24481 agagagaaaa cgagtgatgc gacaagtaca gaatctggag tatcaatatg ttgtagcgac
    24541 tgatttagct gctagaggga tcgatatcga aggtgtctct catgttatta atgcagaaat
    24601 tccagaagat ctagacttct ttatccatcg tgtcggacgt actggacgta atggcttaaa
    24661 aggaacggcc attacgttat attcgccaaa tgatgagcaa gccattgatg cgatagaaaa
    24721 attgggcgtc tcatttgaac caaaagaaat caaaaacggg gaaatcgtca aaacatacga
    24781 tcgtaataga cgtgccaaac gagaaaaatc aaaagatgta ttagatccca cgctgattgg
    24841 tcttgtgaag aagaaaaaga aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc
    24901 aattgcagaa aaaaataaaa aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc
    24961 tcgtaaaaat aagaaaaatt cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt
    25021 cacaatctct tgtttttaag ctagactttt tttcattccg atacaaagta cttcatcttc
    25081 aataaaaggt tctgtgcaag tatgataccc ttgtttttca taaaaatcta cagccgtcat
    25141 ttcagctact acataactag tctgacaacc ttctgaaacc gcccgttttt cagcagcttc
    25201 aagcaactga ccgcctaacc cttttcctcg gtattctttt gctacgcaaa atcgttccgg
    25261 cttgaagcaa gtaggtgttt tcttttcata acgcaaagta gctactggtc ttttttgatc
    25321 aagaaataga taataggttt gttgcgggat atcttttccg tcgaattcta gttctgcagg
    25381 aatcccttgt tctaatacaa aaacttgata tctcaaaaag cagctagctg cgcgtatcca
    25441 ttcatccgct ccaactattt ttttcatcat attcctcctt gtcatgaata gcagagatgt
    25501 attgattcct ttattcttat cgtaccaaaa atcgtggagt atgaccaaaa ggaaatgaaa
    25561 ataaaaattg agatattgac agtgatcaat agaatgcgta tactaaataa gtacgaaaag
    25621 gatatgattt gttttttaag actcacagaa aattctttct tggctgaaaa aagaatagtc
    25681 actaatcaaa ttgctccctt tttctatttt ggtagaaata ccgacgcagc tcagcgttat
    25741 cgagtttaag aggtaatgat tacacatcat tgcaatcaag gtggtaccgc gagttttcgt
    25801 ccttggctgt gatggtgttt ttttgtattt aatttataga aagcaggtaa tgacatgaaa
    25861 caattaagta gtgcacaagt tcgacaaatg tttcttgatt tcttccaatc taaaggacat
    25921 acgattgaac ctagtgcgtc tttgatcccc gtgaacgatc caaccttatt gtggatcaac
    25981 tctggtgttg caacattgaa aaaatatttt gatggttcag ttgtgccaga aaatccaaga
    26041 atcacaaatg cccaaaaatc tattcgaaca aatgatatcg aaaatgtcgg taaaacagca
    26101 cggcaccata cgatgtttga gatgctgggt aatttttcga ttggtgacta tttcaaaaat
    26161 gaagccatcc actgggcatg ggaatttttg actgatccaa aatggatggc attcgatcca
    26221 gaaaaacttt atgtaacagt ttatccaaaa gatacagaag caaaacgcat ctggcatgaa
    26281 gaagttggcc tgccactaga tcacattatt gatgtagaag acaacttctg ggatatcggt
    26341 gcaggcccat gtggtccgga tacggaaatt ttctatgacc gtggagaagc ttataacgat
    26401 ttagcagaag acgatcctga aaactatcca ggaggagaaa atgaacgtta tttagaaatc
    26461 tggaacttag tattttcaga attcaaccat aagcctgata acacatttga accacttcct
    26521 cataaaaaca ttgatacggg gatgggattg gaacgcatgg tctcaatcgt tcaagatgcg
    26581 tcaacgaact ttgagacaga cttgtttatg cctattatcc acgccgtaga aaagctaagc
    26641 aaacaagtaa aatatggtga aaacccagta acagatattt cttacaaagt aatcgcagat
    26701 catattcgcg ccctttcatt tgctattggc gacggtgcat tgccatcgaa tgaagggaga
    26761 gggtatgtct tacgccgttt gcttcgtcgt gctgtcatgc atgggaaaaa attaggtatt
    26821 gaacaagcat tcttatatcg tttagtccct gttgttgggg aaatcatggt aagttattat
    26881 cctgaagtac ttcaacaaaa agaatttatt gaaaaagtta tccgtacaga agaagaacgt
    26941 ttcaatgaaa caatcaatga aggtttggaa atcttaaatc aagtgatcca agaagtaaaa
    27001 aacgaaaacg ggacgactct agcaggaaaa gacatcttca aactatacga cacatatggc
    27061 ttcccggtag aattaacgga agaggtggca gaagatgaag gactgaaagt tgatcatgct
    27121 ggttttgaaa aagaaatgga tgcacaacgc gaacgtgctc gctctgcccg cagtactgaa
    27181 aaatcaatgg gggtacaatc tgctttattg acagatatca aagtagaaag taaattcatt
    27241 ggatacacag aacaagaatc agatagtcaa ttattggtca tcgttaaagg tgagtctctt
    27301 ttgaatgaag tcgcagaagg tgaagctcaa atgatctttg atcaaacacc tttctatgca
    27361 gaaatgggtg ggcaagtagc ggatcaagga tggatcatga atgaagtagg cgaaatcgta
    27421 gcagttgtta cagatgttca aaaagcgcct aatggtcaat ttatgcacac cgtagaagta
    27481 aaagcaccaa tgagtgaagg acaaacctat catctattga tcgatcaatc gatgcgcaat
    27541 cggatcatca aaaaccatac agcaacccat ctactgcaca aggcattgaa agaagtactt
    27601 ggcgagcatg caaatcaagc tggttcttta gtcgctcctg gacatttacg attcgacttt
    27661 acccactttg gtcaaatcac ttcagatgaa ttagctgaaa tggaaagaat cgtcaacgaa
    27721 aaaatctggg aagctatccc agttgaaaca attgaaacag atatcgatac agccaaaaac
    27781 atgggtgcaa tggctttgtt tggtgaaaaa tacggacatg acgttcgagt agtaaatatt
    27841 ggcggctggt caatcgaact gtgcggaggc acacatgtag caaatacaga aaacatcgga
    27901 atcttcaaaa tcgtttctga atcaggaatt ggtgcaggcg tgcgacgaat cgaggctgta
    27961 acaagtaaag aagcgtacga actacttgaa gaagaagaaa aacaactaaa acaagtagca
    28021 acaattgtta aatcacctca attgaaagaa gttgtttcaa aagcggaaca attgcaacag
    28081 caagtaagag agctacaaaa ggaaaatgaa caattagcta gcaagttagc gaatcaacaa
    28141 gctggtgatg tattcaaaga tccagaaacg atcaatggaa aaacaatcat tgcagcacaa
    28201 gtaaaagtga aagatatgaa tcagctgcgt caattagctg accaatggaa acaaaaaaat
    28261 atttcagata ttcttgtttt agcaaacgaa caagaaggaa aagtcagtct gctagcggca
    28321 atgacgaaag aatcaaatga agctggacta aaagctggcg acttgattaa agcaatcgca
    28381 ccgaaagtag gcggtggcgg tggtggacgt ccagacatgg cacaagctgg cgggaaaaat
    28441 cctgctggta ttccagatgc acttaaagct gcgaaagaat gggttgcgca acaatagggc
    28501 gtttacgttt gacagaaaaa caaaagttca aacattgaac gaaacatgat tatgggggcg
    28561 aagacaaaca agtcttgccc tcatactttt ttcaattgga tggcgcatct aatcaaatgg
    28621 tcggattcgg atggtcttta gttgacgaaa gtgacagaaa tttttacgat tagtataaat
    28681 aaaaaggggt gaaaaaatga atagtagaaa taagaccttt cgactagtct tacgtgcgat
    28741 cttgttagca atcatcatcg tacaagcgat ggttccttgg ctggggttta ttccattagg
    28801 attcattagt ttaacaatta tccatatcac agtaattatt gctgcagtag tattaggccc
    28861 aaaagatggc atggtgattg gcttgttttg gggaatcgct acaattgttc gtgcctatgc
    28921 catgccaaca acgccatttg atacattagt gttcacgaat ccgattatct cggtagttcc
    28981 cagagtactt gttggcttag tagcaggctt ggttttccac tggatttatc aaaaaaataa
    29041 atcaataacg atcagctcag cctttgcagg ggtactgggt tcattggtca atacagtctt
    29101 agtgttagga ttcatgggat tattttatac aggtgcaacg gcagatgcct atggtgtaga
    29161 ctctgctctt ttatttaaaa ccttagcagg ggttgcagca atcaatggga taccagaagc
    29221 aataggtgcg ggaatcataa caccattact agcaaaagcg ttatttgcag caacaccgtt
    29281 gaaaccagaa taatgaccat cttttaatag taataggacg cacgatagat agaaaaagga
    29341 agagaggaga ggaaaacaat cgtcctctcc atctttttac ctgagcaaca agcaaactag
    29401 aaggggtaaa aaacttggct aagaaacaag aagaaaaaaa gaaacaaata ccgcaacagc
    29461 ggacaaaaaa aggagtacta gcgggtatct taggattggt tacgaatctg atcttgtttg
    29521 tctctaagct aatgatcggg cttttttcag gaagtgtctc cattatggct gatgcagtga
    29581 acagtctatc agatactgct tcatccatat tgactttgat cggatttaaa attgctgcaa
    29641 agccagcaga tcaggaacat ccttatggac acgaacgctt cgaatatatc agtgggttat
    29701 ttgtttcgat catcattact tatgtaggat ttcaattttt agagtcttct gctgaaaaaa
    29761 tcatccatcc agaaaatgtc tctttaacac ccatcgtctt ttttgtccta atcatttcca
    29821 tctttttgaa atttgcacaa ggcaaaatgt atcaggttat tgcgcaatcc attgattctg
    29881 aaacattgag agctacagca acagatagtt ataatgatgt ctttactaca ctggctgtat
    29941 tgctttcagc agcaatcgaa tgggtaactg gatggcgtat tgatggatat ataggttttg
    30001 ctttagcatt atatatcctt tatagtggat tgacgatgct tcgaaatttt gtatacgaac
    30061 tattaggaag tcgcccaacg ccagcagaga ttgaagaaat ggaagagcat ttaaatgctt
    30121 gcgatgatat acttggctat catgatttat tggtgcataa ctatggaccc aataaacgct
    30181 ttgcatcagt ccacatcgaa gtagatgaat caatgaattt gaatgtggct cataagatta
    30241 ttgatctgat agaaaaagac tttaaaaagt ctttggatgt ggaacttgtc tgtcacttgg
    30301 atccaatgcc tgttgataac gaaaaatact ataatatctt gcggcagtta aagcaaattc
    30361 ttcttgaaac aggtacaggg cttaatattc atgattttag agtaattcat caaggaaaaa
    30421 cgctgcaatt tgatgtagtg gtccctgaga aattccagtt tgttgataca gaactagaaa
    30481 gaatcttacg tagtcaaata gaagaaaaaa tcggagatta ccaattggaa attacgtttg
    30541 atcacaatta tcttttatat taaatgagaa actataaaaa gactccttaa agtttttaat
    30601 ttaaagagtc tttttttctt ttaattgttt atggagagcc tgacgtgcaa gattatctgc
    30661 tcctttattt ttgttttcgg gcaaccattg caggatcaat aaagaaaatt cggatagaag
    30721 ttcttggatt ctttgaaggt agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac
    30781 cgtagaaact agtgttttgc tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt
    30841 tttctttaga aattctaaag tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc
    30901 tatagaaagc gggatagata gctgttcttg tttaccttga tagacaatca agattccacc
    30961 tccactagga ccagggttgc cttttgtaga ggcatcaatg tatactttta acataaaaag
    31021 agattctcct ttttgttcaa actaatgtta tggtaagatt atatcattac tgaatcggtg
    31081 agaggttaga gaatgaaaaa aaatattcga tggcagcctg aactggcaca tacgatcatc
    31141 tattggtcat tgaccttgat cattctgttt tttagtctga ttctttcttt agaaaacaca
    31201 ggtccttatt ggaaaagcaa ttttgtttta ggtatttttc ttgtttttct atttctaggt
    31261 ttgcggcgtt cttttatttt aaaatcaaac tctgtgatta tacgttatgc tgctttttgg
    31321 aaagaccgag aaatatctgt cggacagatt cgatctttga cgccaactaa tactggggtg
    31381 caaatgatgt tgcaagatga gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa
    31441 gtagtccttt ttgatcattt tgaaaaaaac ggacccaaat cagaatcaat tccttatcct
    31501 tatacgaaaa aaagaaaaag ctaattttaa aaatcagaac ctaaaaaaac gaatttttac
    31561 aggaaaagaa aagagtgaga cgtacggttc atcccgttta gcgtctcact ctatttgttt
    31621 atctattcat gcatactagt atcggtagtc tgaacgttgc ctttcttgac atgggcagct
    31681 tgtgttcctc gattcccttc gatgatctca aattcgacca cttgattttc ttccaaagtt
    31741 ttgaatccct cttcttcaat cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa
    31801 ctgataaagc cgtatccttt tttgttgtca aaccatttta caattcctgt ggtcaaagca
    31861 ctcacgacct ttctaaagtc tgatcatagc cttcattata aagagtattt ataagtacgt
    31921 caaataaatt aaaaacctgt aattttatgt tttttttctg aataaaaaaa ggtataattg
    31981 gcatatctta acaaagaaga tattttgttg aataaagttc gtgaaaaggg gtaatcaaat
    32041 gaagaatgat ctgcaaattt cacaagaaac agagctagaa ccgattgttg aagtggctag
    32101 taaattagat cttaaggaag acgatttgga actgtatggg aaatataagg cgaaaatcaa
    32161 tttttctgga atcaatcgat caaaggataa cgctgagggg catttgatct tagttacttc
    32221 catcaatcca acaccagctg gcgaaggaaa gtcaacgatc actgtagggt taggagacgc
    32281 gctcaataaa atcaataaaa aatctgttat tgcattgcga gaaccctcac ttggtcctgt
    32341 gatgggaatc aaaggtggtg ctgcaggcgg aggatatgca caggtattgc ctatggaaga
    32401 tatcaacttg cattttactg gtgacatgca tgctatcaca acagctaata atgcattatc
    32461 tgctctttta gataatcata ttcatcaagg aaatgaattg aacatcgatg cccgccgagt
    32521 gatttggaaa cgggtagtcg atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt
    32581 gggtgggcca atccaaggtg ttccaagaga agatgggttt gatatcacag ttgccagcga
    32641 aatcatggct atcttgtgtc ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag
    32701 gatcgttgtc ggctatacat ttgaccgtca gcctgtgact gctggagact tgaaagcaga
    32761 aggcgctctg gcattactgc tgaaagatgc aatcaagccg aatcttgtac aaacaattta
    32821 cggaacacca gcttttgtcc acgggggacc gtttgccaat atcgctcatg gctgcaatag
    32881 tattttagct accaagacag cattgagatt agcagattac gttgtcacag aggcaggatt
    32941 tggtgcagat cttggcggag agaaattttt agatatcaaa gtacctaact tgaaaaaagc
    33001 gccggatgct gtggtgattg tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa
    33061 aaaagatgaa cttaaaaatg agaatcttga tgctttgaaa gtaggttttg caaatttaaa
    33121 gcgccatata cgtaatatgg aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt
    33181 cgtaacagat acagacaatg aactcacact gttggagcac ttgtgtgaaa atcaggggat
    33241 tcttgccaag agagcgagtg tatgggcaga tggagcagaa ggcggcgttg atttagcaaa
    33301 agctgtagta cgattgattg atagaaaaga agctaattac aagccattat accgcctaga
    33361 agaaacgata caagaaaaga cagaaacaat cgtcaagaaa atttacggag gaaatggtgt
    33421 tgttttcagc aaaaaagcgc aaaaacaaat agaagagttt acgaagaatg gatgggacaa
    33481 actgccgatt tgtatggcaa aaacacagta ttcattttct gatgatcctt cactgctagg
    33541 tgcaccggaa gatttcacca tcacgatccg cgaaatcatc ccaaaattag gggcaggatt
    33601 tttagtagcc ttgactggag atgttatgac gatgcctggt ttgcctaaaa aacctgctgc
    33661 attgaatatg gacgtgacaa atgacggaga agtgttggga ttgttttaag cttgattata
    33721 atctaccatc gaaggagctg tattttgact acagcttctt tttttagacg atagggaatt
    33781 aattttgttt acactaggat cattcttttt ctttattgta ttttgagcta tactaaagca
    33841 taataggagg gtatgatgaa aacgacattt ataagaaaaa ccggttttta cggtatgggt
    33901 agctcgattc agctaagaat caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag
    33961 ataacgcttg atctggaagt gccctttacg atgcaggtca ccttttattg gctaaaaagc
    34021 cccgtttata cagtgaaaga agcaaagcct gcttatgtga tcacaatgaa ttacttgatt
    34081 cttcaaatct atccgttttt atttctagca actgggttgg gggccgtttt cctacaaaat
    34141 ttatttttca gtttttttgc agttattgtg ttgattgcgt tctttttcta tataaaaaac
    34201 cgggcatatt tgatcaagga ggaaaattat ggcaaactct gatctttttt taatcagttt
    34261 caatcgaatc gaaaaatgga tgagagacga gatgggaaat gcacgaaata tgggatttac
    34321 cgaacttgtc agacgcctag ctcaaagaaa acaattaatg atcagaaaat acgaagatga
    34381 tcttttacag cttgctcagt taaggaatgc gatcgtccat gatcggatcg cagtcgattt
    34441 tgtcattgca gagccaaatg attggacagt caagcgaatc cagctgatcg aacaagaact
    34501 gattcagcct gaaaccgtgc tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga
    34561 tcttccgtta cttgaactgt taaagatagt agcagaaaaa aggtattcac aatttccgtt
    34621 atacaataaa ggaaaatttg tagcattgat tactttacgt aatatcgggt tttggctagc
    34681 aaaagaaagc caaaaaggtc cggttgattt aacaaataaa cgagcgttgg atttgatcat
    34741 ccaaaacgga aagtacacaa attatcattt tgttccagct aaaacacata tttacgaggt
    34801 tgaagcgatg tttcgtgagc aagggacttt ggaagccgta cttatcacta aagatgggaa
    34861 tcccgatggt aatctgctcg ggatcgtacg acctcgtgat atttataaac aaatagaaaa
    34921 ggattgattt tttgttaatt atttattgga tattcagcgc tttgctagta ggagtggatc
    34981 agttggtcaa atggtggatc actgataatc tagcgcttgg agagacgaaa aatcttatac
    35041 caggtatttt gtcactgaat catattcgaa acactggagc cgcatggagt atgctagaag
    35101 ggaagatgtg gttctttact atagtgacac tcattgcagt cgttgtgatt ctcacattga
    35161 tgattaaaaa tcgtgaaaaa ggtaatcgct ggttcatgat tggattgagt ttgattctag
    35221 ctggtgcgat tggcaatttt atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc
    35281 agacagattt tatgagcttt ccgattttta acgtagcaga tgtgacactt gtatgtggag
    35341 tgatttgtat gttaatttat attattttag atgaaaagga tcaaagaaaa aaatgacaga
    35401 acttaaaacg acgattcacc atgaatctgg taggatcgat aaagtattga ctaatttatt
    35461 caccgattac agtcgttcgc aaattcagct gtggctgaaa aatggagcgg tttctgtcaa
    35521 tggacagcca gtcaaagcaa attacaaagt aaaacaagga gacgagatta ctgtcttgat
    35581 ccccgaacca gaaacattag atgtggtccc agaaaatatc ccgttgacga tcgtttacga
    35641 agatgaagat gtagcggtga taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg
    35701 tcatacgcat ggcacaatgg tcaatgcgct gatgtaccat atcaaggatc tttctacgat
    35761 caacggcgtt attcgtccag gtattgttca ccgaatcgac aaagatactt ccggactgct
    35821 aatggttgcc aaaaatgacc aggcacacga atcattagct cagcaattaa aagataagac
    35881 ctctttgcgc aaatatgttg ctttagtcca tggagagatt cctcacgaaa aaggaaccat
    35941 caatgcgcca atcggccgtt cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg
    36001 gaagtcggct gtcacacatt ttactgtatt ggaacgtttc gaaggattta ctctggttga
    36061 attaaaacta gaaaccggaa gaacacacca gatccgtgtg catatgaagt atatcggtta
    36121 tccgttagct ggagatccag tatacggacc gaagaaaact ttgaaaggca atggtcagtt
    36181 tctccatgcg caaatgttag gttttacgca cccgactaca aacgaaaaga tgatctttga
    36241 agcacctctt ccagaagttt ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt
    36301 ttaatttaga taacggtata gtaattaaca acgaaagcat aaaacttaat agaattcctt
    36361 taagtttagt cccgtgaggc taagaagggt gggagcaagg atacgtatgg tgcgccataa
    36421 gtagattgct cattgacata gtatagctat cagtgtatca gtatacgctc agtctaaaca
    36481 ttcctcctgt cctcttaggg caggagtttt tttatgcaaa aaagtagggg gaatgaaaat
    36541 gcaagcaaaa gaagtagtag atcaggtaac catgaagcga gcattgactc gaattactta
    36601 tgagattatc gagagaaacc atagtatcca agatatcgta ttggtaggta tcaaaacacg
    36661 aggaatctac attgcatcaa gaattgccga acgcctgaag cagctagaag atatcgatat
    36721 tcctgtagga gaactggata tcactttata ccgagatgac aaaaaagaaa atccagaaga
    36781 accggaactt cattcttctg acatcccagt ttccttagaa ggaaaagaag ttattttgat
    36841 tgatgatgtt ttgtatacag gacgaacgat tcgagcagca atggacgcag taatggattt
    36901 tggcagacca agaaaaattt cattagcagt cctggtcgat cgtgggcatc gagaattgcc
    36961 aatccgtgca gattatgttg ggaagaacat tccaacatct cgggcggaag agattttagt
    37021 agagatgcaa gagcttgatg gacaagatcg catcatgatt ttgaaagagg aagattagag
    37081 gagagagaaa catgtcggag aaaaaaggat ttcataatga tgacgtggtg ttagatatcc
    37141 acgatcgccc aaccccagga cattgggtag gattaagttt acaacacttg ttcacgatgt
    37201 tcggagcaac cgtgttagtc cccatcttag taggtatcga tccaggtatt gcgctagtca
    37261 gttcaggact aggtacattg gtgtacttat ttactactaa aggaaagata cctgcgtact
    37321 taggaagcag cttcgctttc attgcagcga tgcagctttt gatgaaaaca gatggttatc
    37381 cagcgattgc acaaggagca gtcacaactg gattagtcta tttgattgtt gcgctgatca
    37441 tcaaaaagat cggttcagca tggctggata aaatcttgcc tccaatcgtc gtcggtccag
    37501 tcatcatggt tatcgggttg ggacttgcgg caaatgcagc aacaaatgct atgtataatc
    37561 agggcacgta tgatttcaag tatattgctg tagcgctgat cacattagga ttaacaattt
    37621 tcttcaacat gtggctaaga ggttttctcg gattgattcc gattttacta ggtattatta
    37681 gcggatacat cgtagccttg ctgtttggaa tcgtggatac taagccaatc gtagacgctg
    37741 cctggtttgc tgttcctaac tttgagattc cctttgtaca atatgaacca aagctatatc
    37801 ttggagcaat cacaacaatg gcacccatcg cttttgtgac gatgacagag catatcggcc
    37861 atcttatggt attgaataaa ctaactaaac gtaatttctt tgaagaaccg ggtcttcata
    37921 agacgttgac aggtgatgga ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg
    37981 taacaagtta cggtgaaaac attggtgttc tggctatcac acgtgtccat agcgtgtttg
    38041 tgatcggtgg agctgcagta tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt
    38101 tgattttaag tatccctgga ccagtcatct caggaatcag ttttatctta tttggagtaa
    38161 ttgccgcaag tggaatgaag attttaatcg agaacaaaat cgattttgat aaaaagaaaa
    38221 atctgttgat tgcttcagtt atcttagtag taggaattgg cggactcgtc tttgaagtag
    38281 gcacatttac attgtcggca atggcattag caactgtact tggcatcatt ttaaatctta
    38341 tcttaccaga aacttcacga agcgaagaaa actaaggagg aaacattatg atcatcaaat
    38401 cagaacgtat cagcttaaag catttattga ccgttgaggc acttactgat caagaagtta
    38461 tgggcttgat ccgcagaggg caggaattca agcaaggtgc taattggact ccgcaaaagc
    38521 gccagtactt tgctacaaac ctctttttcg aaaatagtac tcgcacacat aaaagcttcg
    38581 atgtagcgga gaaaaaacta gggctagaag tgattgaatt tgaagcaagc acaagctctg
    38641 tccaaaaagg ggaaacacta tacgatacag tactgacgat gtcagcatta ggcgtagacg
    38701 tagcggttat ccgtcatggt gatgagaact attatgacga actgatacaa agtaaaacaa
    38761 tccagtgttc gattatcaat ggaggagacg gaagcggaca gcatccaacc caatgcttgc
    38821 ttgatttgat gaccatttat gaagaatttg gtcattttga aggtctgaaa gttgccatcg
    38881 taggtgatat cactcactct cgcgtagcta aatccaatat gcagatgtta aaacgtctag
    38941 gcgcacaagt attcttctca ggtcctagag aatggtacga cgatgaattc gaagtgtacg
    39001 gacattatat gccattggat gaactactag atcaagttga tgtcatgatg atgcttcggg
    39061 tgcaacacga acgccatgat ggaaaagaaa gtttctcaaa agaaggctac catcaggaac
    39121 atgggttaac agttgagcgt gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag
    39181 caccagttaa ccgtgatgta gaattagctg attcattagt ggaaggatta cagtcgcgga
    39241 tcgttcagca aatgagcaat ggagtattcg tcagaatggc aatcttagaa gcagtgctaa
    39301 atggaaaagc gtaattatag gaggatgaaa aaatgaaaac actcattaaa aacggtcaga
    39361 tcaatacacg aaaaaatatg acgacaccag cagaaatttg gatagaagat gggcgaatca
    39421 aagcaatcgg tacaggattc tcagaagccg aatttgatga agtgtttgat gcaaagggac
    39481 aattgatcac acctggttta gtggacgtcc atgttcattt gagagagcca ggatttacat
    39541 acaaggagac gatcgaagct gggactcgct cagctgctcg tggcggtttc actactgtat
    39601 gcgcgatgcc gaatttaaat cctgtacctg atacggcaga aaagctgaga caagtatatg
    39661 acatcattag aaaagatgca gttgtgaagg tattgcagta tgcgccaatc acagaaaatc
    39721 tgcgaagtga gaaattagtt gatcaagaag cactgattga agaaggtgct tttgctttca
    39781 caaatgacgg tgtaggtgtc caaacagctg ggacgatgta tctggctatg aaagaagctg
    39841 caaaaaacaa taaagcccta gtagcccata cagaagatga atctttatta tttggcggcg
    39901 ttatgcacgc tggcaagaag gcagaagaac ttggtcttcc cgggatctta agcgtaaccg
    39961 agtcttctca aattgctaga gatctgttgc tagctgaagc aactggtgtt cattaccatg
    40021 tttgccatgt ttctactaaa gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa
    40081 tccacgtaac agcagaagtt tcaccccatc atcttatttt gatcgacgaa gatattcccg
    40141 aagatttcgg cttttggaaa atgaacccgc cgctaagagg acgggaggac agagaagctt
    40201 tgatcgaagg cttgcttgac ggtacgatcg attgcatcgc tacagaccat gccccccatg
    40261 gattagaaga aaaaagccaa agtttcatga aatcaccatt tggtatcgta ggaagcgaaa
    40321 cagcgtttca actgatctat actcattttg tagaaactgg tcgattcacg ttggaacaag
    40381 tgatcaattg gttggcagtt aaaccggctg agatttttgg tttgaatgca ggaacgctaa
    40441 cggttggtgc ccccgctgat gtagcagttt ttgatattac acaaacttgc acgatcgata
    40501 aagaagactt tttatcaaaa ggggagaata caccttttat cggctggaaa gtaaaaggcg
    40561 aaacacaaat gacttttgtc aacggaaaac ttgtgtggca gaagggagaa taaaagtatg
    40621 gaacgtttgc ttatattaga agatggaaca gttttcgaag gaaaggcttt tggtgcagat
    40681 gctagtgtct tcggagaaat cgtttttaca acaagtatga ctggttatca agaaactatc
    40741 actgatcaaa gttttaacgg tcaaatcatt acatttacct atcctatggt gggtaattat
    40801 ggggtaaata gagatgatta tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa
    40861 gaacatgcac gtgtagcttc aaactggcga aaccaaatga cgctagattc atttttgaaa
    40921 caaaaaggaa ttcctggaat atcaggtatc gatacgcgcg cccttactcg caaaatccgt
    40981 gaagtcggaa cgatgaaagc tagtatagtt gatgctggag attccttcga acatgcattt
    41041 gatcagctga aagcaaccgt tctagcaacc aatcaagtgc agcaagtctc aactaacaaa
    41101 ccttatccaa gtccaggaac aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat
    41161 agtattttac gagaattgag taaaagaaac tgtaacctca cagtacttcc ctataacaca
    41221 gatgccgaga cgattttagc gctatcacca gacggagtca tgctgacgaa tggacctggt
    41281 gatccaaaag atgtgccaga agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg
    41341 atttttggta tttgtctcgg tcaccagtta tttgctttag ccaacggtgc agatacctat
    41401 aaaatgaaat tcggacatcg aggattaaat cacccggtac gagaagtagc gaccggaaga
    41461 attgatttta cttctcaaaa tcatggctat gcagtcgatg aagccacgat tgatccagaa
    41521 aaactcatcg taactcatgt agaagtaaat gacggaacag tagaaggagt caaacacaga
    41581 gattatcccg ctttcagtgt gcagtttcat ccagatgctg cccctggtcc gcatgatgca
    41641 ctacatttat ttgatgaatt tatggaaatg atggacgcag gaaaggagca gtaaacagat
    41701 gccgaaacgt acagatatca agaaaattat ggtgattgga tctggtccga tcatcattgg
    41761 tcaagccgca gaatttgact acgcaggaac acaagcctgc cttgccttaa aagaagaagg
    41821 atatgaagtg gttttagtaa attctaatcc tgcgacgatc atgacggata aagaagtggc
    41881 agatcaagta tatatcgaac cgatcacact tgaatttgta tcaaggattc ttcgaaaaga
    41941 gcgtccagat gctctccttc caactttagg cggacagaca ggcctcaata tggcaatgga
    42001 gctggcagca tcaggtattt tagaagaatt gaacatagaa cttctaggaa caaagctttc
    42061 tgcgatcgat caagcagaag atcgtgatct attcaaacag ttgatgaaag aattgaatca
    42121 gccaatcccc gagtctgaaa tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa
    42181 aatcggctat cctgtcatcg ttcgtccagc ctttacttta ggcggaactg gtggaggtat
    42241 gtgtgaaaat gaagaagaat tacgtatcgt atcagaaaat ggattaagtc tttcccccgc
    42301 tacccaatgt ttgattgaac gaagtattgc aggttttaaa gaaattgaat atgaagtgat
    42361 gagagattcg gctgataatg caatcgttgt ctgcaatatg gaaaattttg acccggtggg
    42421 tatccataca ggtgattcta tcgtatttgc ccctagtcaa acattgtctg accatgaata
    42481 tcaaatgctt cgtgatgctt ctctttcgat tattcgagca cttaagatcg agggaggctg
    42541 taatgtccaa ctcgctcttg atccacatag ttttaattat tatgtgattg aagtcaatcc
    42601 gcgtgtttct cgttcatctg cgttagcaag taaggcgact ggctatccga tcgctaagct
    42661 agctgctaaa atagcgcttg gattaacatt agacgaaatg aaaaatcctg ttacaggaac
    42721 gacttttgca gaatttgaac ctgcattaga ttatgttgta gctaaaattc ctcgctggcc
    42781 gtttgataag tttgaaaacg gagaacggct tttaggcacg cagatgaaag caactggtga
    42841 agtgatggct atcggtcgga atatcgaaga gtctttattg aaagctgtgc gttctttaga
    42901 aatcggtaca caccatatcg aacttcctga gttggcagac atcacggacg atgacctaat
    42961 ccaaaaagtg gttcgagcac aagatgatcg cctgttttat gtagctgaag ctattagaag
    43021 agggtacacc atagaagaat tagcggattt gacaaaaatc gatttattct ttttagataa
    43081 actgttgcat atcgtagaaa tcgaacagca gttgaaacag cagccgttgg atgacaagat
    43141 attgaaaaca gcgaaacaaa acggttttac agacaaaaaa gtggcggagc tgtgggaaat
    43201 aaacgaagaa gccgttcgcc agatgagaaa agaaaatgat ttgattcctg tatataagat
    43261 ggttgacact tgtgcagcgg aattcgaatc aaaaacacct tatttttata gttcctatga
    43321 atatgaaaat gaaagttcac gatcagaaaa gccctcaatt ctagtactgg gatctggtcc
    43381 tatccgtatc ggacaagggg ttgaatttga ctatgcgact gtgcattctg tcaaagcaat
    43441 ccagcaggca ggatacgaag caatcatcat gaacagcaat cctgaaacag tttcgactga
    43501 cttttctatt tcggataaat tatattttga gccattgacc ttcgaagatg tgatgaatgt
    43561 gatcgagcta gaacagccga taggagtaat cgttcaattt ggtggtcaga cagcaatcaa
    43621 cttagccgaa cctttgactg ctgctggtgt aaaaatctta gggacaacga tcgaagactt
    43681 ggatcgtgcg gaaaatcgtg atttattcga gcaagcgctg aaagagttgg atattcctca
    43741 acctttaggg gatactgcta caagtaaaga agaagccgtg gttatcgctt cgagaattgg
    43801 ttatccagtt cttgttcgtc caagctatgt attaggcgga cgtgcgatgg aaatcgttga
    43861 aaaccagcga gatttagaag attacatgga acatgctgtg aaagcatctc cagaacatcc
    43921 agtactagtg gaccgttact tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg
    43981 tgaaacagta ctcatcccag ggatcatgga gcatattgag cgtgctggtg tccattcagg
    44041 ggattcaatg gctgtgtatc cacctcaaac attatctgaa tcgatcaaga aaacgatcga
    44101 agattacacg atccgcttag cattaggttt gaattgtgtc gggatgatga atatccagtt
    44161 tgtcatccat gaaaatgagg tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt
    44221 tccattctta agtaaggtga caggaattcc aatggcacaa attgcgacga aagctatttt
    44281 gggagagaaa ctaaagacac ttggttatga aaacggactt tatccagaaa caaaagaagt
    44341 tcatgttaaa gcacctgtct tttcttttac gaaattgcag aaagttgata cttacttagg
    44401 gcctgaaatg aaatcgactg gagaagtaat gggatctgac agacaattag acaaagcttt
    44461 gtacaaggca ttcgaagctt ctggattaca cttgcctgaa tatggagccg tattatttac
    44521 gattgcggac gaaacaaaag aagaagcact agaattagct aaacgtttta cagaaattgg
    44581 ctatagtctg ataggaacga aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt
    44641 tgaaacagta gcgaaaattt ctgaggaagc agcagaaaaa aatgtagtcg acttaataag
    44701 agatggtaaa acacaagtgg ttgtcaatac gattgataaa gatcggggaa atgcttctaa
    44761 agacggtttc atcatccgaa gagaagcagt tgaacatggt attccattgt ttacttcttt
    44821 agacacagca gatgcgatcg ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat
    44881 ttaacggcga gcacatgtta agacaaaaag acgaaaaacc gttgcaggag gggacagatg
    44941 aaacaagaaa tgatggaaat cgttcagcaa aaacagctgg caccaaagat ttttgaaatg
    45001 acattgaaag gtgatctagt aaaagaaatg cagcaaccag gacagttctt gcatattctc
    45061 gtgccgcgtg cagacttgct attacgccga ccgattagct tgaatcatat cgaccacgaa
    45121 acaaatactt gccgtatcat ttatcgggtg gaaggtgagg ggactagagt cttctctact
    45181 ctttctgccg gagaccattt agatgtcatg ggtcctttag gcaatggttt tgatttgtcc
    45241 aatcttcata caggagacga agtatttata atcggtggag gaatcggtgt ccctcctcta
    45301 tatgagttat caaaacagtt agtcgaaaaa ggaattaagc caacgcattt tttaggtttt
    45361 gccacacatg aagtcgttta ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc
    45421 gctactgacg atggaacata cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg
    45481 aagacggaac ctacagctgt ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa
    45541 cagctgtttt cttcgcatca aaatgtccag ttatcattag aatcccgcat ggcatgtggg
    45601 ataggggctt gctatgcttg cgtttgtcat acgccggaag atgaaacagg aacaaaaagt
    45661 gtgaaagttt gtgacgaagg accgattttt aaaataggag aggtaattat atgaatgaga
    45721 atcctttagc ggtcaaactt cctggcctag aattaaaaaa tcctatcatg ccggcaagcg
    45781 gctgtttcgg ttttggcaaa gaatacggaa aatactatga tttgaatcag cttggatcga
    45841 tcatggtcaa ggcaacgact ccaaatgccc gctttggcaa tcccacgcca agagtggcgg
    45901 aaacaccatc tggcatgttg aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta
    45961 tgcataaact tcttccagaa ttagaggagt acaatgagtt acccatcatc gcaaatgttg
    46021 ctggtgcttg tgaagaagac tatgtagaag tttgtagcaa aattggcgaa gcaccaaatg
    46081 tgaaagcaat cgagctgaac atttcttgcc caaatgtcaa acatggcgga attgcttttg
    46141 gaacagattc tgatgttgct tttcaattaa cacaagcagt aaagaaagtt tcttctgttc
    46201 ctgtctatgt caaattgtcc ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg
    46261 aagctggagg ggcagatggg tttacgatga tcaatactct tcttggtatg cggattgacc
    46321 ttaagacaag aaaaccgatt ttagccaatc aaacaggtgg actctccgga ccagcaatca
    46381 agcctgtcgc tattcgtttg attcaccaag tagcaagtat atccagcctt ccaatcattg
    46441 ggatgggcgg cgtgcagaca gtggatgatg ttcttgagat gtttatggct ggagcaagtg
    46501 ctgtagctat agggaccgcg aatttcaccg atccgtatat ctgtcctaaa ctaattaaag
    46561 agctccctgt gcgaatgagc gaactaggca tcgaatcgct ggaaagatta atcaaagaag
    46621 tgagagagga acgtgaacga tgaatcaact agaccaacga ccaattattg cattagattt
    46681 ttcaactagg caagaggtag aagactttct tcgatttttc ccgaaagaag aaaaattgtt
    46741 tgtgaaaatc ggaatggaat tgttctacca agaaggacct gaaatcgtac gttatctaaa
    46801 agaggctggt cataatgtat ttttagatct gaaacttcac gatattccga atacggtcga
    46861 aaaagcaatg cggggattgg caaagcttgg tgttgatctc acctgtgttc atgccgctgg
    46921 cggtattcgt atgatggagg cggcaatgcg aggattagaa gaggggactc cagaaggtgg
    46981 aaaacgtccg ctacttctag ctatcactca gcttacttct acaagtgaag aagaaatgca
    47041 tgccgatcaa ctaatcgaag tttctttaga aaaaagtgtg atccattatg ctagctgtgc
    47101 caaaaaagca ggattagatg gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga
    47161 aatggcagga gacgaatttg tttgcttaac tccgggtata agaccagaag gaacagtcgc
    47221 aggagatcag acaagagtag tcacaccttc tcaggcaaaa aagatcggct caacttttat
    47281 cgttataggt cgaccaatca ctcagtcaac taatccttat aaagcatatc aaacgattca
    47341 aacagaatgg agctaaccta aaatgaatgt tgaacaaagt atcgcaaaag acttattaga
    47401 aattgaagca gtatttttaa atccaagtaa tccttttaca tgggcaagtg gaatcaaaag
    47461 cccaatttac tgtgataacc gaatcacgat gagctatcca aaagtccgaa aagagatcgc
    47521 aaaaggatta gcaagcaaaa tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac
    47581 tgcaacagca ggaatccctc atgccgcatg ggtagcggaa atactggatt tgccaatggt
    47641 ctatattcga agtaaggcga aagatcatgg gaaaggtaac caaatcgaag gacgtatatt
    47701 tgaaggtcag aagatggttg tgatcgagga tttgatttct acaggcggaa gtgtcttgga
    47761 agcagctgaa gcagcaaaaa gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac
    47821 gtatgagttg ccaaaaggaa aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt
    47881 gaccaattat tctgtattga ttgaagcagc tttagaagat cgttatatcg atgagcaaga
    47941 attaactctg ctaaaagaat ggaaaaaaga tcctgaaaat tggcagattt aagcgcgctt
    48001 gatgaagata gagaaatccg atttgtgtct ccagaatcat caattggaat agcaaaaatc
    48061 caaaaactga ttgcgagaag catcttttca tcaggtatga aaagaaactt ctatcgtgat
    48121 cagtttttga tttttttaaa attcattttt ctaagactgg ttagcagaaa gaagagaata
    48181 atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa gggagctgtt
    48241 tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat atgatattcg
    48301 gaaaaagaaa tctcaaaggg aggtaacttt tatggggttg aatgaattta ttttaggatt
    48361 gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag agcatacagt
    48421 tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc
    48481 gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc atgactacac
    48541 agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg
    48601 gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag agattccagc
    48661 tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg
    48721 agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg atgaaatcat
    48781 caaaaataac ccggaaaaag tttataagga gatgttcatt gaggcagtga ctacgattaa
    48841 aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc cggaactatt
    48901 gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa
    48961 agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa agaatatgga
    49021 tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt tatgtgattg
    49081 gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca agggggaaga
    49141 aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt ttactgacaa
    49201 ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg tagagttcaa
    49261 gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac atatcatcaa
    49321 cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa aagagaatct
    49381 agttgtgggt gtcatgtacc agctaatggt acaggaaggt tggctgtata atcaagaaaa
    49441 tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg tgttctttcc
    49501 ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta ccagcgggcc
    49561 aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagagtttc ttctcccctt
    49621 cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg gaagagaaat
    49681 ttattatttc gaggagtttg agcaatgaat attcagcaga tgaaatacgt ggtcgctgta
    49741 gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca gccaagtctt
    49801 tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac tcgaacgaat
    49861 cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga gaaaatattg
    49921 gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga caatttttcg
    49981 atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact cgaggaattc
    50041 aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt gatcgaagaa
    50101 gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa tcaagctggt
    50161 atcagaagat atttacaaca aggagagcta agtggccgca gcattggaaa attcaaaacg
    50221 catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac caaagaagaa
    50281 ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc gtatttttat
    50341 gaagacctta tcgagatccc tgatcaagaa acagtgattt atacgagtga tcgaggtact
    50401 ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat tgtgattgga
    50461 gggattcgcg agcacttgcg gttaatccct ttggcagaag gcgaactaaa tgaattttat
    50521 atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat caaagagctt
    50581 actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt tattgaacac
    50641 cgttgatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt tttgtagcaa
    50701 acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta gaaacactca
    50761 ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc acgataatcg
    50821 tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt cggatatgct
    50881 ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa aaataggcag
    50941 ctaactcggc agcttctgta attgtttcat ctgagggctt gtcactttta atgatgacgt
    51001 gcgaaccggg tatatttttt gcatgaagcc aatagtcggt ttttttagct gtcttcattg
    51061 tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg tcagaagaaa
    51121 agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc ttctttttca
    51181 ggtatccttc tgaagtcaat tcttctttaa ttgcttcgat atccattggg ccggcaattt
    51241 ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct tggatttgtt
    51301 cgccaatcag cttaacagcg ttcttcagct tttggtatcg atggaaatat ttctgtgcat
    51361 tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct tcttcataat
    51421 aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta
    51481 gttcgccatt tctacggtaa ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt
    51541 ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc agttctccgc
    51601 cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct aataattgac
    51661 tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga taagcaatcg
    51721 gtgtgaaaaa atctttattt tccgtttcgt aaaaagtagg aataggctga tggctgatgg
    51781 ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt aatcttttgg
    51841 tcagctcttg agcgatatcg aaaccgattc cctgaaactg acgttggatt gcttttggat
    51901 ctagttcagt ttgggaaagc cgctgaaaga ttttttcttt ctcggagcta aatgggttca
    51961 agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa cgataagtgt
    52021 tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct cgattaacta
    52081 agacgatcgt actatgacgt cccattaatt caacgatcaa tatgatattt tgtagatcac
    52141 ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg atttgttcga
    52201 tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag ttaggcggat
    52261 tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt gggtgcgcag
    52321 ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt tcattttcat
    52381 aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat tcgttgacca
    52441 tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt ttcataagta
    52501 tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc
    52561 tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa atccaaattt
    52621 ttaggtataa taaaaaatag gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt
    52681 agttaaatcg agagtacaag aaatgaatga acatttctca gtgtcattcg cagaaaatcc
    52741 aagtaagtat tttcgacgtc ttgtttttct gctcatctta tcattgctgt taacagttgc
    52801 aatgttttta ttgccggaaa aaatccgaga agagcaagta aatagtctcg tttattcgtc
    52861 cataccaacc agcaaattga aaccttttga ttacagcaaa gctgatcaga aaatcaaaca
    52921 aagccgggcg atatcggtga tgttcagcaa accgaacggg aaaacctatc aggacatgct
    52981 tgatgttttt aatgatcctg agcttatgga agaattgaat cgaccgatct tttattatcc
    53041 gatcgtttat gatgtacatg aactggaaca aaaatataac atacgaacgg atgaagtgac
    53101 atttattttc tttgatggag gaaaagaagc aaatcggata actgcgggta agggaggaat
    53161 aactgatttt gataaggagt tgatccctga actgaatcga cttccgttag ccaatatcaa
    53221 acaattagaa gaagaattgg atagaacggg agtaacttcc caaaattccg agattgctaa
    53281 ttgagacatt ggcgcgatga aacggtgggt ctatatagcg ctatttttaa ttatcagaac
    53341 aagtgatgtt tgatcttcaa aataacaaat aaaaaattaa aaaatatagt tgacgaaatt
    53401 caaaaattcg attagactta aatcaattct taaggaaagg gtgacctgta atgaacttat
    53461 caaaacgtat ttcagcagaa aacaaaaaaa attggcgcag ctggcgtaga taagttgtat
    53521 taatggtgag actttcccat agatacaact ttttagagtg catttctaaa gcttgtatct
    53581 atgctagaca tgaacattga ctgccgcata gattagaatc tatgcggttt tttgtgtaaa
    53641 gacaatgacc gctttggcca ttgtcttttt ttgtagactg gatacttgcc tgattataat
    53701 aaataaaaag attgactttg caggaggaaa aaaatgaaaa acaaacgatt aataacagtt
    53761 gtagtaatta ttttagttta tttagcaggt gcatttattt atggaaaagc ctcaggcgaa
    53821 aaagaaagta cttcgacgaa ccagacaaaa aaaacaacag ttgttggggt attgcaatac
    53881 gtgagccatc cggcattaga tgagatttat cgaggaatca aagatggtct gaaagaagaa
    53941 ggattaaaag aaggagaaaa tcttgagatc aaatttcaaa atggacaagc cgatcagagc
    54001 aagctagcca caatgagtca acagctagta caatctgatc cagatgcctt gattggaatc
    54061 gcaacacctg ccgctcaatc attagcaaac acgacttcat cgatcccagt tgtgttaggt
    54121 gctgtaacgg atcctgtggg ggctggatta gtcgcttctt tagagaaacc tggtggtaat
    54181 gtaacaggtg tttctgatca gccgcctgtt acttcacaaa tcaagcttgc ttctgatttg
    54241 ttgccaaatg caaaaaaagt cggaatcttg tatgcttctt cagaggataa ttcaaaatac
    54301 caagtagcac aagcagaaga agctgcaaaa aaagaagggt tagaatcagt aaaatatgcg
    54361 gtgccttcca ctaatgagat tgcacaaacg gttcaagtga tgagccgaca agtcgatttt
    54421 atctatgttc cattagataa taccattgcc aatgcgatgc aaacagtggt aaaagaagca
    54481 aacaaagcaa atatccccgt cattccatct gtggatacaa tggtggaaca aggtggacta
    54541 gcaacaatcg ggatcaatca atatcaatta gggcttcaaa gcggtaagat ggcggcagaa
    54601 cttgcgagtg gaaaagaaaa accggaaacg acgcctgttt atacatttga tcaaggggat
    54661 acagtgatca atcaatctca agctgatcgc ttaggaatca ttgttcccca atcgatgaag
    54721 gaaaaagcca aaatcattac agatgactcg cagcaagaaa catctaagga ggatgacaaa
    54781 tgatcgtatc agctatttca caaggcatgc tttgggcaat attaggatta gggattttca
    54841 tgacttatcg gatcttagac tttcctgata tgacgacaga agggtccttc ccattaggtg
    54901 ctgctgtctg tgtgacagct attactcatg gggtagcgcc gattgtagct acattattag
    54961 gcgtaggtgc tggcatgata gcaggactcg ttacggggtt actttataca aaaggaaaga
    55021 taccggttat tttagcaggg attttagtga tgtcaggatt gaactcagtt attttgtttg
    55081 tgatgcgaac accaaatctt tctttattga atcatccgat tctacaagac agcttccatc
    55141 gctttgggtt acctgattac tttgacacag tccttcttgg cttcatctct ttgggcatct
    55201 tgattgctgt actgctattc ttcttcaata cagatttggg tcaaggctat attgcgacag
    55261 gagacaacga aacgatggct cggtcgttag ggataaaaac cgatcgtatg aaaattttag
    55321 gtttaacttt gtcgaacggt gtcattgcat tatccggtgc gctgattgcc cagaacgacg
    55381 gatatgcaga tgtaaataaa gggattggcg taatcgtcat cggtcttgct tccatcatca
    55441 tcggtgaagt gatcttccaa gaattgacgc tagcagaaag attgattgct attgttgtcg
    55501 gcagtattat ttatcagcta ttgattttag tagtaatcaa actagggttt gacacgactt
    55561 atctgaagtt gttctccgct atcatcttag ctatttgtct gatgatcccg caattgaaga
    55621 aagcactgaa cttaaaaacg ggttttgaga aggaggcata gtaatgactg atatattgac
    55681 aatcaaaaat ggcagaaaag tcgtaaacaa tggaatgaat gaagagaaag tactattgga
    55741 tgaaattaac cttacaatca gagaagggga cttcatcact gtccttggcg ggaatggtgc
    55801 tggaaaaagc acactgttca atacgatcgc tggtacgctt caactcagtc aaggatcgat
    55861 ctcttttaaa gatcagctga tcacaaaaga ttcagaagaa aaaagagcac gattcctttc
    55921 tcgagttttc caagacccca agatgggaac ggctcctcgg atgacagtag cagaaaattt
    55981 gctacttgca caaaaaagag gtcaaaaacg gaaattccgt ttacgtaact tgaaaaatca
    56041 aaaagaagat tttttcagac tatgtcaaga agtcggtaat ggcctagagc aacacttgga
    56101 tactccagcg ggtgaattat ctggtggtca acgccaagca ttaagcttgc tgatggcgac
    56161 gatcacagct cctgagttac tattattaga tgaacatact gcagctttag atcctaaaac
    56221 agctaaagca ttaatgcgat taacaaatca gcggatcaat gaacaaaaat tgacttgtct
    56281 catgatcacg catcgaatgg aagacgcctt gaaatacgga aatcgtctga tcgtactcca
    56341 aaaagggaaa atcgttcaag atatttctga aaaagaaaag caacatttga caatgggcga
    56401 ccttttgcag ttctttgaag aagtagtgga ttgatttcga cagaaaaatt cctttcagta
    56461 gaaggacttg aaaactcatt gacatcttcc tttgattcat cctatactta aaacaataaa
    56521 caattaaaca tatttgggtg ttttgtagga tcacgtagtc aatgatttta acaattaaat
    56581 agaatattct ttgctctgac aagtaaacga tgagaaaaga atttgctact ttgccaccta
    56641 gacgtttcgt actttttcgt atcaaacgct aagtggcttt ttttattgaa gtaagaacaa
    56701 aggaggaata agatgatttc tggacaaacc aaattagctg ggctctttgc tgatccggct
    56761 tctcacagtt tatccccatt gatgcacaat actgcttttg aagcaaacaa catcgatgcg
    56821 gtgtatttag ctttccaagt aaatccaatg aatttgaagc aagcagtcga aagtattcga
    56881 acttttgata tgttaggtgt aaatctttcg atgccgaata aaacagcggt tattccttat
    56941 ctggatgagg taagccagga agcgcaattg atcggagcag tgaatacgat cgtgcacgaa
    57001 aagggtcgtt tgattggcta taatactgat ggtgcgggct tcatgcaatc cgtgcaagaa
    57061 gctgggattt cgatcaaaaa gaaaaaaatg actctactgg gagctggagg tgcagcaaag
    57121 gcagtcactg ttcaagcagc gttagacggc gctgaggaaa tcgtagttta taaacgaaac
    57181 aaccaaacat tcgctcaagt aaaagaaaca ttcgaacaac tcagctatcg aacttcttgt
    57241 aagataaaag ttcgtgactt tgccgatacg catcaattaa gaaaagactt gaaagaaagc
    57301 tgtcttctag tcaatgcaac agatatcggg atgggaaaga aaatcaatca gtcaccgctc
    57361 ttagatataa gcatgatgca tgagaatcta gcggtttttg atttgattta tgcacctaga
    57421 gaaacacgtt tattaaaaga agcaaaaaaa gtaggagcaa aaggatacaa tggacttggc
    57481 atgttgatct accaaggagc aatcgcgttt gaattatgga caaaggaaaa gatgccgatc
    57541 catgtgattg aaaagctgtt tgattaaaaa aggataatag ataaagatct tttattctcg
    57601 cataaacggt ctcaagaaaa gtaatccttt atgtatcgaa gcatttattt cgttattttt
    57661 tattaaatta tcaaaaaatg ataggctttt gtcgattggt tatcgtatcc tttatttatc
    57721 agtaagaaac gaggaggaac cacaatgagt cggatggatc gctataagga catccataag
    57781 aaggcgaaac ctctgaagcg ggatacgggg agtatttttg ctagaagaga acgaaaacga
    57841 acgcgtatgg aaaatgagga agatgctacc cgtatttatg aaagagagca gcattcattt
    57901 gaacatttga accaatacga gcaacagata tacagagatg caccaagagc agaagaacaa
    57961 caaaaaaata aaaaattttt caatcggcaa aagaagaaca aaccgttaaa acctcgaaga
    58021 aagcgatcgt ggaaaaaaat cattctatcg attttgcttg ttttgatcct ttattcaggt
    58081 atctcatttt ttattggtaa atatgtagct gaacatgaca caagcttaga ggcagcgact
    58141 tcagaaacct ttcatgggca aaaagcgacg aatggtgcgc ataatatttt gatccttgga
    58201 agtgatacac gcggagaaga tgccgggcga gccgatacga tcatggtctt acagttggat
    58261 ggtccttccc ataagccgaa actgatttct tttatgcgtg acagcttcgt tacgatccca
    58321 ggggtaggac aaaataagat caattcagct tatgcatacg gaggagccga tctagtgcgt
    58381 caaactttag tagaaaattt tggaatcgat tgtcaatact atgcgaaagt cgatttcaaa
    58441 tcgtttgaaa aagtgatcga tgccttattc atgaacggag tgaagatcga tgcagaaaaa
    58501 gatctgaatc tggatggtgt agatattaaa aaagggggcc agaagatgga tggacatgtt
    58561 cttttgcagt acgcacgttt ccgaatggat gaacaaggag actttggccg tgtcagacgc
    58621 caacagcaag taatgaacgc tattttcagc caattgaaga atccattgaa tctgatccgt
    58681 gcaccttatg cagctggtaa agctttagga tatacatcca cagatgtcac tgcttggttc
    58741 ttgacgaaaa acctcttttc gattgcacga ggagctggtg gcattgatcg aataagtgtg
    58801 ccagtagacg gttcgtggaa ttatgggcaa agtgattatg ctggcagtat tcttgaaatt
    58861 gataatcaaa tgaatcaatc tgctattaca gattttttga ataaatgatc ataaaaaaga
    58921 tcaagaagaa gctgcgcaag gcttttgtca tgaatcaatc aaacgattca ctcgtaaaaa
    58981 acgctttctt atggtagatt taaccaagag aggagctgtt tacattggat atctatctga
    59041 acttcaaaaa tgagtcgcgt gaagccatcg ctttttatga aaaagttttt cagactgaag
    59101 catcaggtat caccacatac ggtgaaatgc ctggaataga acaaatgaac ttgccagaag
    59161 aagcaaaaaa tctaatcacg aatgcacggc tgaacattta tgatacatta gttatgtttg
    59221 cagatacgcc agaaggaatt ggtcctgaat tagttgtggg aaataatgtt tctttagtca
    59281 tccagacaag aaatgaagaa gaaacgaagc gcctatttga aagcttagct gagggtggta
    59341 agattgagat gccactggat gctgttccgt gggctcagtt gtatgggatg gtaactgatc
    59401 gatttggcat caactggcag attaaccaag agtgacagct aaagatgaat attttctgga
    59461 aacatcaagc agcaaacgga tcatcgtttg tttgcttgat gtttttgtgt ctatttcgac
    59521 tatacggtat ctttttttta ttgccagatg cgaaaatatg ttccataata gagaagtccg
    59581 caaaaaatta agtaacggag gtcagaagat gaagacggtg atactagctg aaaaaccttc
    59641 acaagcaaaa gcctatgccg aaaccttcca acagcaaaag cggcatgaag ggtattatga
    59701 aatcagcgat ccattgtttg ctggtgaagt aaccatcact tatggttttg gacatttggt
    59761 agatatggtt gctccaggag cttatgaaga gcgttgggca aaatggtctt tagaaaattt
    59821 gcctattttc ccagaaacct tccgttacga agttccaatg gacaaacgtg cacaattttc
    59881 gatcgtaaaa cgagaattgc agtcagccga taccatcatt attgcaacag atggtgatcg
    59941 agaaggtgaa gccatcgcgt ggtcgattat tcgacaagca aacgcatttt ctaaggaaaa
    60001 gaactattta cgtttatgga tcaattctct tgaaaaagaa gcaatctatg aaggttttaa
    60061 gaatctccaa cctggaaaga actattttcc aaaatataaa gaagcgcaag caaggcagaa
    60121 cgctgattgg ctgattggaa tgaatggaag tccattatat agcttgctac ttcaacgaaa
    60181 agggattgat ggcagttttt ctcttggtcg tgtacaaaca ccgacactct atatgattta
    60241 tcagctgcaa gaagaaatca aacacttcaa aaaagaaacg tactttgaag gagaaggttt
    60301 aattacgaca aacaaagggc agttttctgg aaaaatcgta ccaaaaaaag catttaaaac
    60361 ccaagaagag ttaacgaagg aaatagaaaa acttggagcg catttaggta agcaaaacgg
    60421 acggattctt gaagttacga aaaaggaaaa gaggttgcat agtccacgtt tattttcgct
    60481 atctagtttg cagacaaaga tgaatcagct aatgaaagca agtgccaaag ctacacttga
    60541 tgctgctcaa ggattgtacg aaagcaagtt tctaagctat ccaagaacgg attcctttta
    60601 cattacagaa aatgagcatc agtatctggt taaacatctg caaaattata aaaaattttt
    60661 aggaatagaa gaagtacaga caactgagat caaagcaaag aaacgatatg tcgatgcgaa
    60721 aaaagttcaa gaacatcatg caattattcc gacaaagacg attccgactc ctgcacgctt
    60781 tgcgaagctt tccaaattac agcaagcaat ctatttgcaa gttttgcgca ctactgtggc
    60841 tatgtttgct gcagattata tctacgaaga gacgaccgta atgacgggcg tacagcaatt
    60901 aaagctaaaa agcatcggaa agatcccatt acagcaaggt tggcaggaaa tcttacgcat
    60961 caagtcaaaa aagaaagaag ctcagactct tcctcctatc gtagaaaatg aagaagcaga
    61021 agtagaactg aaaagtatgg agaaagaaac acaacctcca aaaccctata ccgaaggaac
    61081 gttgatcaca gcaatgaaaa ctgcgggaaa aacgctggat aatgatgaag cgcaagaaat
    61141 tctcaaagaa gtagaaggaa tagggacaga agctacacga gccaatatca ttgaaaatct
    61201 taagcagaag cattacatcg aagtcaataa aaacgaaata actgttacgc caaaaggaat
    61261 cactctttgt aaggcagtag ccaatgaacc attactgacg agcgcagaaa tgactgcgcg
    61321 ctgggaaggg tacttgagaa aaattggtaa acaagagggg acaccatctg tttttttaga
    61381 gaatatcaaa aaattcatcc tacatttatt ggcagaagtt cctaatcaaa tcgacaaggt
    61441 agacttttca gatgagatta gcgggacacg tcagataaaa gcgatggaga agaaaaacga
    61501 tcaattagga agatgtccaa aatgtaaaaa gggaatagtc atgctttatc ctaaaatcgc
    61561 tacatgcttg aatccggaat gcgacttcaa attgtggcca acagtagcta aaaagaagct
    61621 gactaaaacg aatctcagag atttgctttc aaaaggaaag acaagtaaag ttgtcaaagg
    61681 ttttactggt aaaaaaggga aattcgatgc agttttagta ttaaaagaag atatgacaat
    61741 cggtttttct tttccatgga tcgaaaacag tggaacaaaa gcagttgatg gaaacaaaga
    61801 aacatcaaga aaaaataatg aaaacgaagt agagtaaatg aaaacaatga aatagcggaa
    61861 tatttattcc gaaaagtgag aatataaata ccaataaaac taaaaaatat cagtgaatca
    61921 agcaaaaacg ttggggtagg ctgattttat atagatagca aggagttttt ttatttgaaa
    61981 aaagtgcatt catttttaaa ttatcttatg ggagcaatca cgattgtggc tgtgtaccaa
    62041 gcatttctaa agtatcctga ttacgggacg aagttgttgt cattgacgca caatcaaaca
    62101 gcatattcac caacgttgat tttatcgact ttgactgcag gagctaaaag cgctacttat
    62161 ttttttcata aaacgacaag catgctaaag cgaaagaaac agcgaaaaca attgttggtg
    62221 gtggtagcgg ctatgatcat tatttatttt ctaccagtta ttacacaagt atttcatcaa
    62281 tattttggtt aagaatacga gagtttgtga caaaagtcat gtcacaaact tttttctttg
    62341 tcattttttc gtaagaaaaa gcaaaataaa agtatataca ttgattaaaa gtatatactt
    62401 ggatataatg tacttactat agaatgagaa tggtatagag ggtggcgaaa tatgaaaaaa
    62461 atgaaaaagt ataaaattag aaaatctggc aatagcgatg taacaactat cccaccagaa
    62521 gtaaaagaat tcatgggtgt gcagacaggt gacgcaatca gttatgtttt tcaatctgat
    62581 ggttctgtac gaatgatcaa agcacaagaa gaacctgata ttgattcatt agttgattcg
    62641 atcatgaatc aatatgaaga cgcgttaaaa gatttggttg atttatgagg tatctttcga
    62701 ctaaagaaat tatcaaaatc aacgcgaaag ttattttacg tcactcgcca ggcgaaatga
    62761 tcggtgtaaa agatgcaaat gcattagata tggccgtcaa acagccttca caagcagtat
    62821 tcaatcaaga actctatcct gaaatctatg aaaaagcggc gattttagca attaatttag
    62881 ctaaaaaaca tccttttcat aatggcaata aacgaactgc tcttgttgcc atgttgttgt
    62941 ttcttcaatt gaataattgt ccagttgctt tttctcgtca agaagctgtt gattttatca
    63001 taatgattac cactagttca ttggatttcg attcactaaa gtccaagata accagttatt
    63061 tacgacaaac cgcaataaaa tgaacgaatt aaaaacttta tttctcaatt agatcagcat
    63121 gtcattaagg aatatgctga tttttttatg tttaatcata caaaatagtt ttttcataaa
    63181 aatatggaaa atatggatta attttgaaat aattgattta aaaggaatac aaaagagtga
    63241 atagtaggaa atagtggttt agaactagtg ggctgatttt ttatatattt tagtttacat
    63301 aatatatatt atacaaagta gatttaaaaa atgataaacc tctctcgaac ggttttttat
    63361 caaaaatcgt catttttcca tcaaaatctg cttatgcaaa attataaaaa aattttgcag
    63421 tttttcttac tttttttgtc gtttaattga atgataaaaa ccagtttctt tattcagtta
    63481 atcaaatatg aacgaattag atatgaatca ttgtatttgc cgttgcggaa ggatttttta
    63541 attaaccaca gataaacatt gataacgatt cgcttattgt aatcaacaga ttaaatttca
    63601 tatgttcata tgtacggata cattttcgga ccgattaatt ttaaccattc tgtttatttg
    63661 cttacctatt agatatcaat aaaaaactgc ttaacagcta tttcagctgt taagcagttt
    63721 taggattttt agttcatatt cattgtttct tataatacag tgttcaatac ttctcttaaa
    63781 gcacttgttg atttgctgaa cattgctttt tcttcttcgt tgattgataa ttcaacaact
    63841 tcacgaacac cattttcgtc aacgattgat ggcacacctg taaagatatc tttttcgtcg
    63901 tattcaccag ttaaataagc agatacaggc aatacagctt gttcgttatt caagatagct
    63961 tttacgatac gtgcagtact cataccaata ccatagtaag ttgctttttt acgatcaatg
    64021 atctcataag cagcattacg gactttatca gcaatcacat ctaattcatc ttgggcaata
    64081 cgatggtctt tttctacaat ttcaaacact ggtttaccac cgacagttgt atgtgaccat
    64141 gcagcaactt ctgaatcacc atgttcacct aagatatagc cgtggacgct gcgtgggtca
    64201 acttttaatt tcaatgcgat ttctttacgg aaacgagttg tatccaatgt tgttccagta
    64261 ccgataacac gtgatgttgg cagaccagat tcgttccaag cgatatatgt caaaatatct
    64321 actgggtttg aagcaacaac gataataccg tcgaatcctg atcccatgat ttcttttacg
    64381 atttggcgca tgattgacgc atttgtttta actaaatcta aacgagattg accaggtttt
    64441 tggttgatac cagctgtgat aacaacgata tcagcatctt tacattcttc atagccacct
    64501 gaccatacag ccacattttc atcgccccaa gccataccat ctaaaaggtc caatgcttcc
    64561 ccttctgctt tttcttgatt cacatcgatc aatactaatt cattagagat tccttggttg
    64621 atcattgcat aagcgatact tgttccaaca aaacctgtac cgacgattac tactttacga
    64681 cttgtttttt tcatgaataa acagctcctt ttcataacta tttgttactt tcatgaaaag
    64741 tatagtcttg tttattcatt ttatcaacat gttaattata atagttgtta ttataatttt
    64801 tgatggaaac gaattcctga atacgcataa cagataaatg acaaatgttt acacctatat
    64861 cccctgataa tacaaccaaa agtttagaac tgaaaataaa tccaaaaaaa ttcgctgcaa
    64921 cgatataata agacagcaat cgcacctaaa acaattttcc ttcatatgct ttagtgaaag
    64981 attttcttct taatgtttat ttctccattc attgggagta catgttgaat acttcttgaa
    65041 taatttgaat aaagaagtcg tttgtttata tccgatacga ttagcgattt cttccaacga
    65101 aaaactcgtt tcttgaatca gttccaacgc aaagtcgaat tttttcttta gaaccagttc
    65161 ttgaaaggaa gcatcaaaat acattttcaa tttattacta agatagaccg gattgtagcc
    65221 aaaatgagct gctgtttctt ttaacgtaca agaagtaaaa tgcttatcga tataatctaa
    65281 tactctaagc atctcatttt ctgcgcatcc atctgctgat gaaaagcttt gttgttctaa
    65341 taaatgagtc aattcgatca gtaatagtga tgtatagaga tttaacgaac ggatcttatg
    65401 actgtatgta tcaaaataaa tcaacagcat attttcaatc aaatgcgtca aacgacttgt
    65461 ttttccgccg cgaaaatgaa tgaattgtgt atgttcccct ttttcttttg atgcatttat
    65521 caaaaaagaa gaaagtagat tatttgtgct tgccaaatga ttcaatatct ctgtagataa
    65581 ggtttcttct tttaataaga tgttgattaa aatatcctct tgccctgtgt attcgattct
    65641 ttgagtagct tctttatcaa tgatcaccac gtcactttct tccatctcta tcctttctcc
    65701 attaataatt tgtaaacatg taccgctata catataattg atttcaacaa aatcatgtgt
    65761 atgctcggga acttccgcat atcgacggtg tttactgata tgaatatttc cctgttgaaa
    65821 tagatcccct cggtggattt ttggcggtgt ctgaagatgg taagctaagt ttaaatcatt
    65881 gatattcatt ccttgttcac gctgcagagt ttcgattgat tcttcgttac gcaaaaaaaa
    65941 tgtctaattc agctagttcc aattgactcc ctcctttttg taagcggttt ctttcgctta
    66001 ttagttcacg ctgtactgct attatatgca aagctatttt tggtttcata aagactttct
    66061 atggttggga catgaacttc ctgtgattcc gctattgaga gaataaccac ttcatactat
    66121 atttttgata agcgcttaca aaaatgaaag gaacatgctt atgaataaaa agatgatgaa
    66181 attttctctg ctgtcagtct ctcttatttt gacttctgct ggtgcagtca cgtctgcaat
    66241 tcccagtatg tcagctactt atgccacgcg ttctttatct gctgttgaat tattaacgac
    66301 tgctccctct tttatggtca ctatttttgt tttgattagt agttttattg ctaggaaaat
    66361 cggacaaaaa caaactgtga tcatcggatt agtgattgca tcgatttgtg gtatcctccc
    66421 cctcttctca caaagttaca gtattgtctt agcttcaaga gctggactag gtattggctt
    66481 tggattgatc aattctttgg cagtcagcat gattgctgcc ttttatcacg gagatgaacg
    66541 agcctctatg attggttttc aaagtgcatt tcaaggattt ggtgcagctt taatgacatt
    66601 tgttgcagga cagctagtac agttcggttg gcaatatacc tttttagtct acgccatcag
    66661 cttacctatt cttgtcttgt ttgttttgtt tgttcctaca cctgaaacaa ctgaacttca
    66721 atcgcatggc acagccccaa caagacaagt aatcaaaacg aatcctaaaa tgattagtta
    66781 tggtattttt ctcttgctgg tcatcgtggt atataatgct gtatcgatta aattagccac
    66841 agtcctgacg acttattctc taggaaacga aactaatgca gcgaatatct taacaatcat
    66901 gcaattggca agtatggctg ccggagtttg ttttggctgg cttcagacaa aagcacatcg
    66961 ctatatgctt acactttctt tactactgat gggaatcggc tttgtattga ttgccactgt
    67021 gataaacctg tatgtgattg ggtttggtgc gatcctaaca ggaatttctt tttcgttatt
    67081 cattccttac ttattcaacc aagtaagtat ccaagcgccc aagtacgcgg agagtttcaa
    67141 tacttcaatc ttattggttg gcgctaattt aggtactttc attgcaccat atggtttgct
    67201 ctttctctct tttgcttctc ttgggagtaa aacacttgct gttttcgtca atggcagtat
    67261 cttgctgttg attagcgcgc ttattagcct tctagtagta cacaggacga atagaaagac
    67321 agtgataaga gtagatgaag aaataaaaga acaattgaca acaaaataaa tttgatggag
    67381 gtcgtgtaaa atgacaaatg aaaaacataa agatagatgg ctttggtatc ctggagactt
    67441 tgagatccgc catggactgc tacaaaattt ccaacgggaa gaacgaggtt ttgactggcc
    67501 agcgtactgg tacatggatg attgccatcg gaacgtcaag tttaagagat attactttct
    67561 tgatcaacct tccatgttta aagtgacgat ccaaggagtt gggtatgtag aaattaatgg
    67621 acaaaaacat ccatgtggaa aatggctgac ttgtccagct ggtaaagcaa aaattcgtat
    67681 ctttgtagga catacatcag ggctaccagc gatgtttatc gagggagacg aagtaaaatc
    67741 agatattggc tggacagcaa gtaattttat tgaagaatat ccagctggct ggagtccgct
    67801 ttatgtcgat atcgcaaaag atccaaatca aatctattat caaaaagaat gcgtccatcc
    67861 agtcactgaa acggagattg gcggaggaat cttattcgat tttgggcgag cagtgaatgg
    67921 ctgtgtagaa ctaatatctc tggatgacca tatcgaatct atcactctat gttatggaga
    67981 gtccgatgta gaagctatgg atatcgatta ttgctactat aagcaaatag gaatgacaag
    68041 aaaaatgaaa gctcgtaaac gcgcttttcg ctatttgttt ataccagagg tttctactgg
    68101 aatggttcaa cttcaagttt ttcatgaatt tttatctctt tcgaaacaag ctgctttttc
    68161 aagcgattct tctttactga atcagatctg gtcagtatca gaagaagcct tcaagttatg
    68221 tagtgatttg ttttttatcg acggtatcaa acgagatcgc tggatttggt ctggagatgc
    68281 gtatcaatca ttcatgatca atcaatatct tttttttgac gaggaaatca ataaacgaac
    68341 gatattagca ttaagaggac aaaatgaaat caagcagcat atgaatacta tcgttgacta
    68401 ttcgatcttg tggttgattg gtatcgaaaa ccactatatg atgacgaaag actatgaatt
    68461 tttacgcatc gtctacccta agatgcaatc attaatgaga tacttgatgg agcaaacgaa
    68521 tgaattaggg tttatttatg ggagagcaaa agattggata tttattgatt ggtctgaaat
    68581 ggataaagaa ggaacgcttg ctgcagaaca agtcttatta ctgaaagcct atcattccat
    68641 gatcacttgc ggagatatct tagctgaaac atatggagag aacgaaaatt atcatactca
    68701 tctctatcaa gaaaaatatc aactattgaa acaaaaccta tttgactact tttgggatga
    68761 agaacttggc gcttttatcg attgctatga atctgggaga agaaatgtga cgcgtcatgc
    68821 gaatattttc gctgttttat tcgattttgc taacaaagac caacagcaat cgatcttaaa
    68881 gaatgtctta ttgaatgaca ctatcacaca gattactact ccatatttca aattctatga
    68941 acaagatgct ttatgcaaac tcggacagac gaatatcgtc taccaaacga ttcttgatta
    69001 ttggggaggg atgttatctc atgatgcggt aactttctgg gaagaatacg accctactca
    69061 aacaggaaat gaaaagtaca gtatgtatgg agatccttat ggaaaaagct tatgccatgc
    69121 atggggagca agcccaatct atcttttagg acgctatttt atcggtttgt atccaacaaa
    69181 gccaggttat gaagaattta tgatacaacc taatctagat tcatttgaaa cactgaattg
    69241 tacgctacct atcaaagatg gaaagatttc gcttgaaaaa acaaaagata agatcgtgat
    69301 tacttctgat agaacaggcg gaacagtgaa aatgaatggt aaaagttatc ctcttctagc
    69361 aaaacagcca cttgtcttaa caagctattc aaaaataaat gcataaaaca aaaaactgct
    69421 tgcgacatat cagaagactt taaatcttct tctgatattg tccaagcaat ttttattttg
    69481 aagtttgctt aattctgttc tttagaaaca atgtttgttc tttttccggc aaaaagaaag
    69541 ctagataata cgacaagtaa aaagaaagcg atgattccaa ctgtcaaggc tatttgaccg
    69601 tattctccta gtttcccaag tacaagtcct ggtactagat aatcagtatc ggcaaatgta
    69661 gaagaagcga ttttaagatc tcctaatact ggaaggataa ataatggcag ccaactaatc
    69721 aataagctat tgaaagcaga acctaatacc gcccctcttc taccgccagt cgagttgccg
    69781 aagacaccag aagcagctcc gcagaagaaa tgtcctacta ccccaggaat gataactgta
    69841 gtttgcaagc ctatcatagc aaacatggat agtgttccta caaagaagct aacaaaaaag
    69901 ccaattaata cagcattagg tgcatatgga aaaacgatag gaacatctaa tgctggttta
    69961 gaatcgggta cgagtttttt agcaatccct tggaatgctg gaacgatttc tgccaacacc
    70021 attcgaaccc cttgaaggac gataacaaac ccagctgtga atgttccagc ttggatcagt
    70081 gcaaagatta cagcgttgtc tccattgctt agttctcttt caacaaatcc aggaccagct
    70141 aacaaagcaa gtgcgacata tacgatcgac atcaagagca tgatcgatac agtagaatct
    70201 cgcaaaaagc tgaaactttt aggaatcttt atgtcctctg tagaacggtc tttgtttcca
    70261 aaaaactttc cgatccatcc actagaagca taaccgatat ttccggtatg ccctaacgca
    70321 acttgattgt ttccagtgat gttcttcata aacggttgac aaagagcagg cgtaatagtt
    70381 aatagtgttc cttcgaagac cccgcctaat aagatcatcc acaaatttgc agtaaacccc
    70441 gcgcttacta aaatgactgc tgttagacaa gaaacataaa gcattgcttg acctgtaaga
    70501 aaaatgaatt tatatcttgt gaatctagca agtaaaatat tcacgatcat ccctactagc
    70561 atgatcaaag cagtaggtgt accgtattgt tgcaaagcaa tcgccactac tgcttcattg
    70621 ctaggaacta ctccttgagt atgcagagct gtttggaaca tcgaagcaaa aggggttagt
    70681 gaattactga ttaaaccagc accgccgatc agtactagga atccgacaaa tgttttgata
    70741 gttccttgga tcacttctga aaatttcttc ttctgaagaa cgagaccaag cagtgcaatc
    70801 aaagaaacga ggatggaggg ctgactgacg attgacacaa acaaattcaa caatttatcc
    70861 attgatagtt cctcccttga atcattttta ttcttttgac aagatcttcg ttactttttc
    70921 ttttacttct tcgctgtcaa tgatcgattg caaaggtacg agcttttcat caggtataga
    70981 agttagcgta tctttcaaag aatgctcaat gaagaaataa tcagccatat cgctagatac
    71041 acttccaaca tccgtatgac ttacttcaaa ctcgtccaca tctgctccaa gatctgcaag
    71101 tgcttgtttg atgttcatct ctaccataaa acttgttcct aatccacttt gacatactgc
    71161 aaaaattttc atatcattcg tctccttttc ttatgatcgc taatatttct tcttttgttg
    71221 atgcgtgtaa taaatcttga agattttgtt tattagaaag tagctttgtt aaatttgcca
    71281 aagcccgaag atgtgcctca ttatcgatag cagctaaaca gatgaacaaa ctaatgggat
    71341 gggctggatt atcaagcaga tccacaggtt gttgaagttt cagcaaagac atccctactt
    71401 cgtttacgcc atcttctgga cgagcatgag gtaatgcgat gtgatcgcca atatggataa
    71461 acgcaccata ctgctccacg cgctcgatca tagctttgat ataactttct tctattttat
    71521 tttctgataa tagcggctgt gcggcaaatg tgattgcttc ttgccagttt tttggttgat
    71581 ccgataattg gatcatttct tctgttaata attcagatag cataggacgt tgatcctcct
    71641 tcttttctaa aagtttgttc attttacgat ttaatacttg atagagcttt tcttcgtcta
    71701 cgccttcttt taattcgata taaggcagta gcgccgacaa caattcttgg gcagaaggca
    71761 ttgaaaatcc tggtatcagc caatctcttt gtacttgatt gatcagctga tttttttcca
    71821 agtcagtcat gattggatgc agaacataga gagacttttt cttatcagta agtgtgagag
    71881 gtacagtaga aaaaataata tcgtaagaag tttcgtcaac ctcgtgtaag cggtcagctg
    71941 aattagtctc tgtaaaaaag atcatcggga ataattgctg aagttcactt tttaagatca
    72001 atgaagagct aatgccactt ggacagacga tcacggctct tatttttcta tttctttctt
    72061 ctgcgtccgc ttttctgatc tctccgccaa atagtatagt gaaaaaacca atctcttctt
    72121 cagggatgct cttccctatt cgtctttcta acggaagtaa tgcttttctt gtcatttcgt
    72181 agatggaagc atattgatgc ttaatttgat cgatcatcac attaggtaaa taaaaagaat
    72241 aggaaatacg aaaataggca gggaccaagt gataaaagac attcatcaat aattcacgaa
    72301 acgaagcaaa ttctacggcc atcaggaatt ccatgcgatg gatgacttct gaagaacatt
    72361 ccagcaaaaa atcaagtgca ggctcctgga catctccttc aacgatcgat gctaagcaaa
    72421 gagagaaaaa tataatctcc gaattttgat gttcggaata gccaaggcca gtcaatactt
    72481 gtcgactaat gtcagtgact gcaagagaag ccaacagttc agcatctttt accagttcca
    72541 tggatgtttt tccaattcga atggttgtca aactcaaata ataagctaat tcatccattc
    72601 gatttggtgc gaatcgaaga tctgatagtg aaatcgtttt atctagcaaa tctctaactt
    72661 tggcgtaata atcaatggaa agggttgcgg ttaaatacgt gagcccaaat cttccagcaa
    72721 gtgactgcat caaacgagca gtgcgatttt ttgcttctct tcttaatatt gcttctgacc
    72781 ctagcagcct aaaaccagtt ttcctagtat acgacagctg acaatcttcc acttctaatt
    72841 catttcttat ctttttgata tctgacagga ctgtcccttt agaaacctga agaaaatctt
    72901 gaaaatgaaa aacagaaaca gacgttgcag tcgaataaac catcaaataa agaagatctc
    72961 ttcgctcttg ttcggtatac acagcttcaa atcgagtaat atccaaaaat ttttctgccc
    73021 agtcgattgc tcggaatttc tttatacgga tacgggtatc atccacatat tcggcaaaag
    73081 gagttaattg tttgatcgcc tcgcttgtat ttacttcgat gatctctgct gctttttcta
    73141 gactgatttc tcttggtgtt ttcaggagat aactaatttt gtagttcaca gcgctgttca
    73201 cctcctactt atattctagt atggtagcgc ttttaaatcg aggggtgttt ggtacaactt
    73261 acagttggat tttttttacg actgtaccga acgatttcta tcgaatggat acagaaaaaa
    73321 agcaggtgac caaatgtctc ctgcttttat gaattgattt cgtccgatca atgatgatca
    73381 tgtttatttt ttgaggtcag ttcttcaaat ttttctttga catcttcaac cgctttttcg
    73441 atatcttttt caaagtgatt gggctcatta ggctttgttt cctcttctac agaatttgaa
    73501 ccagataaag ctgtttcttt attttctgtt tcaccagagt ttgtgccagc taatgattct
    73561 tctttgtctt ctgtttcacc agaattagtt cctgctaaag ccacttcttc ttctgtttta
    73621 ggggccattg aacctcttaa tcgtttgtgt ttttcaccgt cataatttgt catgttggtt
    73681 cctcctagta gttctatact ttttcatcgt acaataagac agtttatgcg tcaaaaaaca
    73741 aacccgattc ttacaaatat gtcatatttt tccttctgat tcttgaatgc ttttgattcc
    73801 ttcttccaaa attttcattt gtaagattgt ttcttcatct ttgatgactt tttctgcgcc
    73861 attaactaat gtctcgtaaa tcccttcgta gacacggctg taatcaccaa cctcggaaat
    73921 tactttttct tcatgataac tcccttgttc atctatatag gtcaatacac cataatgttc
    73981 gggcaaatcg ataccaaagt cttcatgatc aggcatgtaa aacattttca gatgttcttc
    74041 ttgtcgatct tttgtttgtt tcacgaacat cccttttttt ccgtaaacga cgaaacttgg
    74101 acgttctttg atgcggaaat aactggattt tatcgaaatt ttcatatttc catagaaaaa
    74161 atccagatcg aagtaatcat tcattcgtcc tttccctaaa agttgacgta catcatagct
    74221 gatatcatct ggttcgccga aataagaaag aacttgatct actgtatgac aagcatgtcc
    74281 atataaatag ctgttcgcag tagaaaattc atgcgtattt tctggtactt ccggtctgaa
    74341 ataatcataa tgcatttcta cttctaaaat atcccctaat tttccgcttt cgattacttt
    74401 ttggactgtc aagaagtcag aatcgaaccg tctgttttgg taacattgga taaatagacc
    74461 tttttctttt gctaatgcaa ataactcttc tgcttccttt gacgtttctg caaaaggttt
    74521 ctctactagt acattcttac catgttcgag tgccattttt ccatattcat aatgaaattg
    74581 actaggtgta gtgatgacaa ctaattggat ctcaggatca tcccaaagat cagacagctg
    74641 atcagtatat tcgatgtctt cgaatgtttt ccacactggt tcagatgggg aaaaaatttt
    74701 tttgatattg attttgttcg taagttttct tgaaaaaggc aaatgatagc gatttgtact
    74761 ctttccattg ccaagataag cgattgtaag catgagtggt gcctcctttt attgatgttt
    74821 tcagtatatc ctagaaaagc gcagaaagta tttataatag ctattctggt aaaaaagttc
    74881 agaaaaacac ttgttatcat tcggtttggt gaaattaatg ttcaaagtaa ggataaaaat
    74941 caaaaagata gtagcggaac caagtgttta tcagtaagat agaatttgga ggtcagatca
    75001 tgcttattga agaaaaacta acaaaacaag agttgtttac cacgacagag aaacggattg
    75061 tcgattacat ccgtaggaat atcgaagctg cagtctacat gacgattgaa gaattagcaa
    75121 aagctacgta tacttcccat tcagctatca ttcgtttatg caaaaaaatg ggattcagcg
    75181 gatttaaaga attccgtttc gagctggggc gggaagttca tcaattgatt gctcagttca
    75241 atcaaacgat tgatcctaat tttccttttt caagtgaaga tagcactcag caaattgcaa
    75301 agaaaatggc ggatttatcg atccagtcaa taaaaaaagc acaattgcag attgaacatg
    75361 aagatttaga tcaaatcggt aaagtattga acaaggcaca gcgaatcttt ctttttgcaa
    75421 aaggtgattc tcaaatcacg gcaaggaaat tccagaataa aatggtcaag ttgaataagt
    75481 ttttgatcat ggctgaagaa tacagtgatt cttcttggaa tgcagccaat ttgactagcg
    75541 aggattgcgc catttttatt agctacagcg gtcgcatcca tcattatgaa cgaataatga
    75601 cctatttgaa gcatgtagag gcccctacct tgctgattac cggaaatcag cattcagaaa
    75661 tggccaaaca ggctgatatg tgtcttgtta tatcgcagga ggaatatgat tttgctaaag
    75721 tagcgacttt ctcctctcaa attgccttcg attacgtatt aaatacgtta ttctcagtga
    75781 tatattctca aaattttgaa gagaatatcc gcaacttgaa aaacaaacaa gaattgatgc
    75841 aaaaaggcct tcttaaagaa tatcggaaaa cttaagaaaa gatcctcatt tagtaataag
    75901 aaagatagtg tttgataggc tctctttgat atcatcataa aaacaggctg aagttttctt
    75961 tttcaaacct cagcctgttt gtttacggaa cgattcgaat agatatatta tagaaatgag
    76021 gaatagctaa cttctgtttc ttattttgaa cgattgacga aaaagatata taagaaggag
    76081 gaaaatcagt atacatgcag ctaacaatcc atacaggaaa aatcgtttgt tctcggatac
    76141 tttctgtact tctttttgaa cttcttttgc atgataagga acccggtgcc ctctgacaag
    76201 taagcgatga ctgttgacca tataaggtgt acaagttagg agtgtcacga aatcctgccc
    76261 tttttcgatt ctcagatctt ttgtatcagt cggttctacc gtcttgatct gatcgacttg
    76321 ataagcgagt gtccgtccat tgatttcgat atagaaagaa tcacctaaag tcagtttcgg
    76381 taagtctgtg aacaactttg cttgaggcag tcctcgatgg ctggaaataa cggcatgtgt
    76441 attttctccc cccgtaggat aagacgttcc ttccaataat gaggcacctt tttctagcag
    76501 cacagtattc gtttcatcaa aaattggcaa atggacattg atctcaggga tcgtcaaaat
    76561 accgatcgta tgtttctcaa aataggttct atcctttttc gcaggttctt ttttcttagt
    76621 aaagggatct gcacctggat ttccaccttc ttttgctaat tgctggtttt tctttgtcat
    76681 gttttcttgg attttagcca tgacttcttc attttctttg accgcttgtt gctgataatg
    76741 agagatgatc tgctgatcca gataattatt cagcgcgtcg ctaacaaatg gataagctag
    76801 tatccctatt cctgagagaa gcaagaaaag catcagcaga tctagaagtt tccttttctt
    76861 tggtttcatt tgtgttctcc ttgtgatgac tgtttgataa taaaagaaaa aggagcattt
    76921 gcgtaatccg ctgctccttt ttccaattct tgcaaaaaga ctaaatctga ctgtgcttgc
    76981 gtctagcaaa gtacagtcca gcaatcagta gaaggactac tcctgcaccg atatacacat
    77041 agattccctt accgcctgtt gaaggaagtg tacctttgtg tttatttggt attttttcag
    77101 gagaaatcaa ctgtcctgct gttacataag attgttcatc gatagtaaat gctacacggt
    77161 ttgttaatgg aacatatttt tctggcgctt tcgtttcttc cagatagtac gtgccatatt
    77221 ttagacctgt cacatcgatt aaaccgttac ttgtggttgt aaaaaccgtt gctgattctt
    77281 tcgccgatac ccagctgacg gcttttgtgg atgggtcgat cgataaatat ttcgctgtgt
    77341 cactatcttg atcacgaacg acgaattctg ctccagcaag tgtttggtct gatgtaacgt
    77401 caccatctac tttaacaaat cgtttgcccc cagtaacgac atcgactgac ggtggtgttt
    77461 gatcatttgt atgcccgtta tcgacatttg cttggttaga aaatcctttg gttggatctg
    77521 ctttttcgtt caaatgcata tagtaaacga atttcaatgt accgccagga gttaatgaag
    77581 gaatataatt cggatcaact gaaaccgtga atccgtctgt ttgctcagtg acagaataat
    77641 ttactgggtc gatttcttta tttccatcat ataaggcata agcatacgtt cctgaagaat
    77701 cattatcaaa tgttaaagca gcgtcatgag tatcgataag tttgaatgtt gtgtacttgt
    77761 tttgcgttcc ttctttatca gcgataccta atgggatatt gacagaaatc tcatattgaa
    77821 ttttttcacc gattgcgaca tcttttccat tcaattgagg tgttgtttta tcaactttag
    77881 acgtatcatt tttgatggtc ttttctactg gtgtttggct atttgccagg atctcaaaag
    77941 gcgtgattgt ttgcttttca atcatttccg cattatcagg agcttttact tcttctaaat
    78001 tatattttcc aacttctaaa tgattaacga tcaactggcc tttttcaata gatgcctcgg
    78061 caaagtcatt gttgccgatg tcataagaat gaccagtaat gaaatgtttg gctttggttt
    78121 gatcagttgt ccaagtgtac aatccatctg tgacactttg gatgtatttt ttgacgcttg
    78181 gtgttccttc ttctttagaa ataataaatt ctgctccatt tagtgcttcg ttttcggcag
    78241 taccgatttt tgtaactttc aacgttccat cattaccgac tgtattttta gggtagagat
    78301 ggatagtatc tagttcttct gtcccgtatt tataagagcc atctgcttgt ttgatcatct
    78361 catatacagg gaaagctaaa accatgtttg cggcagctga cactccgtct tttggttctt
    78421 ctttgatcgt gtagactgca tctttcccat tttgtttttt aggtaaagat aaagtgacat
    78481 ttccatcagc atctgtcgtt cctgaagcaa ctggtgtacc aggagtcaaa gactggactg
    78541 cttgttttgc tgcatccacg gacgctcctt tatctcgttg cgcataaaat tcttgagtga
    78601 cgttataaac tgaaaatgaa atatcggcta atccttggta ttgatcgaat tcgctcattt
    78661 ctttcccgct gttttggatt aaaggatcgg gtaaatcagt cattttcttt ttatgaagga
    78721 tgacttgagc tgctgtctcc tctgcaaaca ctttttttgc gccgaagctg tttgtgagta
    78781 atggtaaaat aaggaaaagg actcctaaca taacgtttat ttttttatga tttttcattt
    78841 tttcactcct gttctttttt tgataaacaa ccagattgca aagcttgcga ataccatacc
    78901 aacaacgcct aaaagtgtat ttttcgtttc gtttgttttt ggaagaaatg gacggctatt
    78961 ttctttttta ttgactactt tcaatatact gtccgcctct ttatcctcag aaagaaaagt
    79021 tgaaatggta aacggaattt ctgtttcact taaaatataa ccagaaggtg cttcgatctc
    79081 ttttaatcga taacttcctg ttttcaaccc ggaaattgaa aaagcgccat ttttatcgga
    79141 aatcaggtga agcgcatctg attcgttctt tgtccacttg tatccgttgg ctgtttgctg
    79201 gaggtaattt cctgctttgt ttttaataag gaatactgcg ccagctaatc gtttatctgg
    79261 tttatttgtc tcgattttga caaattgctt actccctgta tatacagaga ctttctttgt
    79321 actgacacca aaaccaaaat ccagtgttcc gttgttgttg atttccttgt tcgcctgtgc
    79381 tgtactgctt agctgcatct gataagaaat ggtcagtttt tttccagcat atttttccaa
    79441 cgaagggatc gaaaataaaa catcaaatcc gtgaggtttc ttgtgtatga caaagccttc
    79501 tgtcagcttt tctccattcg atgaaatcgt taaactatta ggtaaaaacg ttaatgcttc
    79561 atccgcacta tctgacaatt cgaacttttg atagtcaagg atatttacag gaattgtcgt
    79621 atgcaaggaa taagtgattt tttcaccaat cgtaaaatca tttctaggct cttctaatac
    79681 cttctcaaaa ggtggatcag ggtagtcgtt ttcttcattt ttaggataca aatggatagt
    79741 tgaaagtttt tgattgtttt gtccatgtac aggaagaaca actaccatat tctctgcctt
    79801 ttctttgacg acttctggta ctttggattc aatgaaaaga tacgctttat ctcttttttc
    79861 ttgatcttta gcggccagag aaaaagaagc gataccatct tcgttgttaa gagttgtagt
    79921 tgttgcttct gcggtaaaca taccggaaga cgcaccgttt tttgcgatct ctgcttgtgc
    79981 ttcttctacc gttttgccct tttcccgtag atggtaaaaa gaatctgtga catcataaac
    80041 ttggaatgtg acaccattta atcctcgata cgtttgtaat aaagcttttt cttgtccgtc
    80101 atttggatga tttttcggca gttgcccgtt tgggaaaagc aatttgtgta aagtaatctg
    80161 aaccatttct gtttctgttt ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa
    80221 gaagagacac atactaagcc aaccaagttt tttcatattt agctgccccc tctttctttt
    80281 ttcaaaaaaa gataccaaat agaaaacgca caaccaatca tccctactag atagattcct
    80341 aaacggccaa ttcctcccgt ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa
    80401 gaaatctggt tgtttttggc tcctggtgac agaacagatt catgatcttg tccatccact
    80461 tgaattgacc catcttcgtg tataactata gtaactggct cttttagacc ttggtatcct
    80521 tctggtgtaa aagtttcggt cagcgtataa gttccaggtt ttaaattttc gaatagaaag
    80581 gtatctgttt cttgtccatc ttttggcgat tctaagtcca tttctggtcc ctgcagtctg
    80641 aattttgctc cttttaatgt ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac
    80701 gcttttagtc gattttgatg agtgagcgtc catacattat ttgtcatctc ggtatttttt
    80761 cccgtgtaaa tgatttttcc atatttatcg atttggaatt cataatttcc cggcgaagta
    80821 tcatatccgg caggaccagc tgtttcaacg actcgatatt taccaggtgt gagtgaatca
    80881 aaactggcaa gacctttttc attcgttgtt tggctgtcaa ttggctgata agtaccattt
    80941 gcttcttttt tctgtaggga aaatgctgct cctttaagat ttatctcttt gtccgtccct
    81001 tgcatcgcat acttacgtac tgctacagga acttcaacaa aaggattttc aatagtcaac
    81061 tggatcgtat catcggaagt agtgactgtt tttccatcaa tggttaccgt accatcagaa
    81121 gcgatcttga tttcccaagt agtctttttg cttaatccat gcccttctgg agcttttgtt
    81181 tctgtcagcg tataggtcat ttctttttgc aatttgacat tttctggaag agaataggtt
    81241 ccgtcgccat gatctgttag taaagtatca atagaatctc ctgttaattt gaaaacagcg
    81301 ccaataagat ccttttcacc tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg
    81361 aattgtttag tatttttcca tgtcattgca tctatttgtt gagaatcgta tcctggtaca
    81421 ggtaattctt tgattgtttg gtaactgaat gcttgacctt gattattgta ttgaggtagt
    81481 gataatatct cttgataact ttctccgcta tttgcagata atttgtcaat gtctgcacgt
    81541 tcccacgtat ttgttgtatc tttagctggt ttaatgattc gaatatatcc gtttttccaa
    81601 gctgcagcat ttgtcgtatg ttcccgttga atctcaaaag taacttgatc tggacgatca
    81661 gctagattgt tgtcaaattc ttcccataac tttttgatgt gaagactgac tccgggagct
    81721 ttagcagatg gtataccgaa ttcagctaat tcattggtat caatgtttgg ctggaaagtt
    81781 gtcctgccgt tcatttgata ccagaaattt ggatggaagt cctcattttc tgtttggatt
    81841 ctcacttggt aatggatttg gatttcttgg tcttttccta aatagatctg attgctcttg
    81901 atggtatttc cttctatgga aatagatgga gtgaccgttg taggacttgt ccccacactc
    81961 ttgactgaaa gtgtaccagg ctgataaatg aatggttctg cgattggatc atttatttgt
    82021 ccattgctta cagttgctga gatctgtaca gcttttttgg ctaggtattc agagatatct
    82081 gttgcatgat cagctgattc atagtaaaga tcgccttttt catctgatga aaccatttgt
    82141 cgcatacgag actctacttc tgcttttgag agaccagctg ccggatcgct ttgaagttgg
    82201 atgccaagac catgtatttc gattcctcgt tctttgagtg ccatcgcttc tccgatggtt
    82261 gcgataaacg tactgtcgat tcttctggat agattgtttt ggtcaggtgc atcatagatt
    82321 cttgagatta gagaagtatt tcccggccga tcttgcgtat tagaaaactg agttccgtaa
    82381 taattgctgc ttgattgtgc gtgtacccgc tgtactttat aggaaaatgt tggtacacca
    82441 tccgtcagca aaacgatcac ttttttatgt ccatttggaa cggatagcat gcttcctgca
    82501 tctcttagtg ctttttgtgt aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt
    82561 acttgatttt tcactgaatc aaatgaaccc atctgtactg caccgttact gtagctatat
    82621 ccttcgcttg agtagccgac atatcccata ttgattttgt ctgtgatacc gctatctgct
    82681 aaagtatcga caaaacgatc gacaccaatc tttacttcac cgatccgatt attgtcgttc
    82741 atacttcctg accagtctac gaccaatacg agatcaagag gcgtgatatc tttttgtaca
    82801 tttccacgag cattcaaata aacatcaaac aatccgggtg tacttgtttc ctttgcatac
    82861 tttcgtatcg catagtctgc ttgattaccg gttcctccat attcgatata cgaatgggtc
    82921 cgatcattag gatctccatt ccaagaattt ataccatccc attgatttgt tccgttttta
    82981 ttgccttgat gatcaagaac atttgtattt cctgtaggtt gccaactggc tttaggataa
    83041 gtgccctgat catctgttgt atattctgta ggaatcgttt gattagatgg cacactcaca
    83101 gctggcaagg ataatgtagg caatgtaaat gggctgacag aattttcgtt ttcttttgat
    83161 gattcttctt ctatttcgtc tggctttggt actgacacat ctgtatcttt cttattttct
    83221 gtctcatcaa taatgtcata ttctttatta tcgacgatca gcttgtttgc ctggctgtgg
    83281 taaagtgctg gatcgattgg aaaactcaga tcgatagttt gtttagtgtt tgatgagatt
    83341 tccaacgtaa ttttcccgct atttacctga taagcatagc catctccttc ttttctttcc
    83401 acatcactta agtaattttc gactagacca atttcttttt ctatcttttc agcactggtg
    83461 ttattcgcat ttagcttgat gttcagtgct ttttttccag actgatccga aatgacttca
    83521 gctgaaattg tttctaaaac tgttggatca tctgtagtgt ctgctattgc agataatggc
    83581 acaaagtttg ataaactcaa tattatcagc atgaaaataa ctttcagttt cttccctgtg
    83641 gtcgtcattt ttcccctcct ttttcttttt ttctgttttg acgtgaatag tgaaaattat
    83701 taaaataaac aggcgttaca taattttatt tattttaata tgataattat tatacgaagg
    83761 gcgctaaagg gaatcacacc aattttcata ccaattggtt tcatttatga agttgattcc
    83821 ttcgattaat gagcgtttca ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg
    83881 ttgttggaac gaaactatag aaagatggaa aaaatcattc gaattctgtc catttctgac
    83941 aagtggatga ctaaaaaaga acttgccaaa cgaattggta gttcagaatc tacatttgta
    84001 aggtatatcg aagagatcaa aatccgctgg ggaacggatg taacgattca atcttcacaa
    84061 aaattggggt atcgtcttga acatttgaat gtaccagctt atttaaatgt tctgatggaa
    84121 atgtcgcaaa gttctacaaa cagtcgttta ttaaatgaga ttattgtcaa tccgggtaaa
    84181 acgattgagt attattgtga aaaaatttcc atcagccggt cttcttttac acgtaagttg
    84241 aaacaatgca atcaaatttt agaaagctat ttattaaaag tgatcgttga ccaaggtttt
    84301 cagttaacta gccttaaaag tgagctccag ttgcgtatct ttgttacctt tttcttttta
    84361 agttactacg gaagacgcac gttgccattt ccaatcgaaa aaaagaagat caaagaaatt
    84421 atggtcaaga ataattgtca gatgaatttg tttcaggaag aaagcccata tgagcaatct
    84481 ttctttatta tgtattttgt tgtgcatctc atgagagaat cccaaggaac tccatttgaa
    84541 tggaaagctg gagactctta tgtacagttg ataaatatcg ataaaaaaga cttcttaatc
    84601 ttgaaaaatc tcttaggaaa attgaagtac agcagctata taaaagtagt gaactatttt
    84661 gtcaatacga ttttttcaga attttcggag aaagaaaaaa atgaaatcag tgagcagata
    84721 cgaaaaaata
//
LOCUS       DACCCS010000003        82424 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.003, whole genome shotgun sequence.
ACCESSION   DACCCS010000003 DACCCS010000000
VERSION     DACCCS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.003"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(60..404)
                     /locus_tag="IVK82_000181"
     CDS             complement(60..404)
                     /locus_tag="IVK82_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP6391277.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(541..1677)
                     /locus_tag="IVK82_000182"
     CDS             complement(541..1677)
                     /locus_tag="IVK82_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP6391278.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(1866..2840)
                     /locus_tag="IVK82_000183"
     CDS             complement(1866..2840)
                     /locus_tag="IVK82_000183"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP6391279.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(2920..3795)
                     /locus_tag="IVK82_000184"
     CDS             complement(2920..3795)
                     /locus_tag="IVK82_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP6391280.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3925..5832
                     /locus_tag="IVK82_000185"
     CDS             3925..5832
                     /locus_tag="IVK82_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP6391281.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(6373..9651)
                     /locus_tag="IVK82_000186"
     CDS             complement(6373..9651)
                     /locus_tag="IVK82_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP6391282.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(9922..10467)
                     /locus_tag="IVK82_000187"
     CDS             complement(9922..10467)
                     /locus_tag="IVK82_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6391283.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(10479..11171)
                     /locus_tag="IVK82_000188"
     CDS             complement(10479..11171)
                     /locus_tag="IVK82_000188"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP6391284.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(11190..11474)
                     /locus_tag="IVK82_000189"
     CDS             complement(11190..11474)
                     /locus_tag="IVK82_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391285.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(11487..12050)
                     /locus_tag="IVK82_000190"
     CDS             complement(11487..12050)
                     /locus_tag="IVK82_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP6391286.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            12278..12940
                     /locus_tag="IVK82_000191"
     CDS             12278..12940
                     /locus_tag="IVK82_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP6391287.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            13064..14260
                     /locus_tag="IVK82_000192"
     CDS             13064..14260
                     /locus_tag="IVK82_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP6391288.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(14361..15737)
                     /locus_tag="IVK82_000193"
     CDS             complement(14361..15737)
                     /locus_tag="IVK82_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6391289.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(15774..16640)
                     /locus_tag="IVK82_000194"
     CDS             complement(15774..16640)
                     /locus_tag="IVK82_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP6391290.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(16729..17754)
                     /locus_tag="IVK82_000195"
     CDS             complement(16729..17754)
                     /locus_tag="IVK82_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6391291.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(17772..18446)
                     /locus_tag="IVK82_000196"
     CDS             complement(17772..18446)
                     /locus_tag="IVK82_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6391292.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(18630..19523)
                     /locus_tag="IVK82_000197"
     CDS             complement(18630..19523)
                     /locus_tag="IVK82_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6391293.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(19597..21294)
                     /locus_tag="IVK82_000198"
     CDS             complement(19597..21294)
                     /locus_tag="IVK82_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6391294.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(21287..22600)
                     /locus_tag="IVK82_000199"
     CDS             complement(21287..22600)
                     /locus_tag="IVK82_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6391295.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(22600..26637)
                     /locus_tag="IVK82_000200"
     CDS             complement(22600..26637)
                     /locus_tag="IVK82_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP6391296.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(26650..27108)
                     /locus_tag="IVK82_000201"
     CDS             complement(26650..27108)
                     /locus_tag="IVK82_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6391297.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(27406..27924)
                     /locus_tag="IVK82_000202"
     CDS             complement(27406..27924)
                     /locus_tag="IVK82_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP6391298.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(27926..28543)
                     /locus_tag="IVK82_000203"
     CDS             complement(27926..28543)
                     /locus_tag="IVK82_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP6391299.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(28540..29403)
                     /locus_tag="IVK82_000204"
     CDS             complement(28540..29403)
                     /locus_tag="IVK82_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6391300.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(29390..30301)
                     /locus_tag="IVK82_000205"
     CDS             complement(29390..30301)
                     /locus_tag="IVK82_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6391301.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(30342..31676)
                     /locus_tag="IVK82_000206"
     CDS             complement(30342..31676)
                     /locus_tag="IVK82_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6391302.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            31884..32714
                     /locus_tag="IVK82_000207"
     CDS             31884..32714
                     /locus_tag="IVK82_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP6391303.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(32958..33207)
                     /locus_tag="IVK82_000208"
                     /pseudo
     CDS             complement(32958..33207)
                     /locus_tag="IVK82_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(33398..33592)
                     /locus_tag="IVK82_000209"
     CDS             complement(33398..33592)
                     /locus_tag="IVK82_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391304.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(34097..34282)
                     /locus_tag="IVK82_000210"
     CDS             complement(34097..34282)
                     /locus_tag="IVK82_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391305.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(35132..35473)
                     /locus_tag="IVK82_000211"
     CDS             complement(35132..35473)
                     /locus_tag="IVK82_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP6391306.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(35473..35703)
                     /locus_tag="IVK82_000212"
     CDS             complement(35473..35703)
                     /locus_tag="IVK82_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6391307.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            35990..36328
                     /locus_tag="IVK82_000213"
     CDS             35990..36328
                     /locus_tag="IVK82_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391308.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(36533..36772)
                     /locus_tag="IVK82_000214"
     CDS             complement(36533..36772)
                     /locus_tag="IVK82_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP6391309.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(36748..37095)
                     /locus_tag="IVK82_000215"
     CDS             complement(36748..37095)
                     /locus_tag="IVK82_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP6391310.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(37827..38408)
                     /locus_tag="IVK82_000216"
     CDS             complement(37827..38408)
                     /locus_tag="IVK82_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6391311.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(38591..39217)
                     /locus_tag="IVK82_000217"
     CDS             complement(38591..39217)
                     /locus_tag="IVK82_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6391312.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(39239..40570)
                     /locus_tag="IVK82_000218"
     CDS             complement(39239..40570)
                     /locus_tag="IVK82_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP6391313.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(40725..41126)
                     /locus_tag="IVK82_000219"
     CDS             complement(40725..41126)
                     /locus_tag="IVK82_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP6391314.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(41212..41418)
                     /locus_tag="IVK82_000220"
     CDS             complement(41212..41418)
                     /locus_tag="IVK82_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391315.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(41513..43015)
                     /locus_tag="IVK82_000221"
     CDS             complement(41513..43015)
                     /locus_tag="IVK82_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP6391316.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(43033..43488)
                     /gene="argR"
                     /locus_tag="IVK82_000222"
     CDS             complement(43033..43488)
                     /gene="argR"
                     /locus_tag="IVK82_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP6391317.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(43586..44203)
                     /locus_tag="IVK82_000223"
     CDS             complement(43586..44203)
                     /locus_tag="IVK82_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391318.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            44424..45797
                     /locus_tag="IVK82_000224"
     CDS             44424..45797
                     /locus_tag="IVK82_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6391319.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            45872..46984
                     /locus_tag="IVK82_000225"
     CDS             45872..46984
                     /locus_tag="IVK82_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP6391320.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(47056..48693)
                     /locus_tag="IVK82_000226"
     CDS             complement(47056..48693)
                     /locus_tag="IVK82_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP6391321.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(48861..49469)
                     /locus_tag="IVK82_000227"
     CDS             complement(48861..49469)
                     /locus_tag="IVK82_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6391322.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(49853..51004)
                     /gene="queG"
                     /locus_tag="IVK82_000228"
     CDS             complement(49853..51004)
                     /gene="queG"
                     /locus_tag="IVK82_000228"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP6391323.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(51023..51775)
                     /locus_tag="IVK82_000229"
     CDS             complement(51023..51775)
                     /locus_tag="IVK82_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391324.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(51916..52866)
                     /locus_tag="IVK82_000230"
     CDS             complement(51916..52866)
                     /locus_tag="IVK82_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP6391325.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(52867..53706)
                     /locus_tag="IVK82_000231"
     CDS             complement(52867..53706)
                     /locus_tag="IVK82_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP6391326.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(53703..54449)
                     /locus_tag="IVK82_000232"
     CDS             complement(53703..54449)
                     /locus_tag="IVK82_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP6391327.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            54687..56057
                     /locus_tag="IVK82_000233"
     CDS             54687..56057
                     /locus_tag="IVK82_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6391328.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(56112..57008)
                     /locus_tag="IVK82_000234"
     CDS             complement(56112..57008)
                     /locus_tag="IVK82_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6391329.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(57289..57966)
                     /locus_tag="IVK82_000235"
     CDS             complement(57289..57966)
                     /locus_tag="IVK82_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6391330.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(58057..58962)
                     /locus_tag="IVK82_000236"
     CDS             complement(58057..58962)
                     /locus_tag="IVK82_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6391331.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            59137..60603
                     /locus_tag="IVK82_000237"
     CDS             59137..60603
                     /locus_tag="IVK82_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6391332.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPPIKKGS"
     gene            60606..61637
                     /locus_tag="IVK82_000238"
     CDS             60606..61637
                     /locus_tag="IVK82_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP6391333.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            61644..62831
                     /locus_tag="IVK82_000239"
     CDS             61644..62831
                     /locus_tag="IVK82_000239"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP6391334.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            62987..64369
                     /locus_tag="IVK82_000240"
     CDS             62987..64369
                     /locus_tag="IVK82_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP6391335.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            64496..64726
                     /locus_tag="IVK82_000241"
     CDS             64496..64726
                     /locus_tag="IVK82_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391336.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(64926..65594)
                     /locus_tag="IVK82_000242"
     CDS             complement(64926..65594)
                     /locus_tag="IVK82_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP6391337.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(65783..66580)
                     /locus_tag="IVK82_000243"
                     /pseudo
     CDS             complement(65783..66580)
                     /locus_tag="IVK82_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(66861..68240)
                     /locus_tag="IVK82_000244"
     CDS             complement(66861..68240)
                     /locus_tag="IVK82_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6391338.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            complement(68415..69584)
                     /locus_tag="IVK82_000245"
     CDS             complement(68415..69584)
                     /locus_tag="IVK82_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6391339.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            complement(69559..70710)
                     /locus_tag="IVK82_000246"
     CDS             complement(69559..70710)
                     /locus_tag="IVK82_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP6391340.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            complement(70670..72031)
                     /locus_tag="IVK82_000247"
     CDS             complement(70670..72031)
                     /locus_tag="IVK82_000247"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP6391341.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            complement(72028..73029)
                     /locus_tag="IVK82_000248"
     CDS             complement(72028..73029)
                     /locus_tag="IVK82_000248"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP6391342.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINENRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            complement(73045..73740)
                     /gene="dapD"
                     /locus_tag="IVK82_000249"
     CDS             complement(73045..73740)
                     /gene="dapD"
                     /locus_tag="IVK82_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP6391343.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            complement(73746..74462)
                     /locus_tag="IVK82_000250"
     CDS             complement(73746..74462)
                     /locus_tag="IVK82_000250"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP6391344.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            complement(74459..75340)
                     /locus_tag="IVK82_000251"
     CDS             complement(74459..75340)
                     /locus_tag="IVK82_000251"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP6391345.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            complement(75343..76143)
                     /gene="dapF"
                     /locus_tag="IVK82_000252"
     CDS             complement(75343..76143)
                     /gene="dapF"
                     /locus_tag="IVK82_000252"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP6391346.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            complement(76130..77389)
                     /gene="lysA"
                     /locus_tag="IVK82_000253"
     CDS             complement(76130..77389)
                     /gene="lysA"
                     /locus_tag="IVK82_000253"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP6391347.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            complement(77875..78438)
                     /gene="efp"
                     /locus_tag="IVK82_000254"
     CDS             complement(77875..78438)
                     /gene="efp"
                     /locus_tag="IVK82_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP6391348.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            78866..79783
                     /locus_tag="IVK82_000255"
     CDS             78866..79783
                     /locus_tag="IVK82_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP6391349.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            79796..80940
                     /locus_tag="IVK82_000256"
                     /pseudo
     CDS             79796..80940
                     /locus_tag="IVK82_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            81111..81569
                     /locus_tag="IVK82_000257"
     CDS             81111..81569
                     /locus_tag="IVK82_000257"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP6391350.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            81728..82198
                     /locus_tag="IVK82_000258"
     CDS             81728..82198
                     /locus_tag="IVK82_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP6391351.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFSKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(82251..82366)
                     /gene="rrf"
                     /locus_tag="IVK82_000259"
     rRNA            complement(82251..82366)
                     /gene="rrf"
                     /locus_tag="IVK82_000259"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        23568 a        17289 c        14663 g        26904 t
ORIGIN      
        1 aaagttagtc atctaacaat aggccgtttc aaaactagtt gtctttttat atccacagat
       61 cattcgactt tttcgaataa cccacggcta atcatcccat ccatcagaaa ataaaattta
      121 tcttggctca ttttctgttt tggatctttg aaaatattga ctgctttcaa tccatttgct
      181 gtagtgatcg acattttcaa tgtttgaata tcttttgata cagtgatttt taatttgaat
      241 ccttctttgc tttgcggcgt ggcatctaat ggtcggatca attgataatt gcctccgttt
      301 gtttctgtaa atccattatc gcgcaatgta tatctttttg catcaggatg caaacgatag
      361 ctaattggtg cgcccagtac cgttgctgtt gtttcaaatg ccatgatttt cacctcgata
      421 aaattagttt ctctttatta taaaagttta tcctgatttt tactaggttt ttcagttaag
      481 ttccctaaaa aatgaaggcg ttctctcaga catctgttca tataagaatg acttcactag
      541 ttattttttg acagcacgaa ctaattgatc aaggaaatac tggatgtcgt cttcggtatt
      601 tcccaaacca aagctgatac ggatggattc ttttagtatc ggtgtatctt ctccatacat
      661 tgcttctaat acgtgtgaag gctcgatatt tcctgctgta caagctgaac cagtagaaat
      721 agcgacccca ttaagatcaa gcttcatcag tagcaaatca tttgcttcgc caattaagcg
      781 aagattcaac acatgaggga gtttattcgt taaatcacca tttacttgat aagggatttc
      841 gtttgcttct aatgtatcaa gaatcaattg ctggaatcct ttatatttgt tgttacgtgc
      901 ttcacgttct tcaggtgaca gcagttcaac tgcttttgcc ataccccaga tccctgctaa
      961 attttctgta cctgcacgcc gcttttcttc ctgctccccg cctaaaagca atgcagggat
     1021 atttactccg tctcgtttat ataagaaacc gacgcctttt ggcccattga ttttatgagc
     1081 agagatactt aataaatcga ttcctaattc ttctggatgg atcgtttggt ttccgtacgc
     1141 ttgtaccgca tctgtatgga aataagctgg atgatccgct aaaagttcac cgatttcttt
     1201 gattggcatc aaatttccga tttcattgtt cccgtacatc acagagacca agatcgtatc
     1261 ttctcgtaac gcttttttga cctcttcgat cgacagattc cctttttcat ctacaggcag
     1321 atatgtaacg tcaaacccct ttgtctctaa atatttcatg gtattaagaa cagcaggatg
     1381 ttcgatagct gtagtgatta gatgtctgcc ttctttttga cgcgagaacg ctgtttctaa
     1441 gatagctgta ttatcccctt ctgttccccc actgttaaag atgatctcgt gaggttttgc
     1501 ttgcagactg tctgcaatga tttgtcttgc tgtttccaat ttttcatgcg cgtggcgacc
     1561 aaatccatgt atgcttgaag gatttccaaa cgtgtcttgc atgattgccg tcatcgcttc
     1621 aattacttgt ggatgcatgg gggtagttgc cgcatgatcc agataaatag ttttcacttc
     1681 attcatccct tccaaattat tgctaggcac attctagcat aaaatagcga acaaaccaaa
     1741 gaagagctca tttttgtcta gaggacgttt catgggcata gaaaaaagga gctgtgacaa
     1801 aagtcttgtc gcagctcctt gaagctgctc acttctgtca caacttcttt ccctcttttt
     1861 tttgtttatt cgaattcttt ttcttccaga cggaataacg gactcatcgg tgtattttca
     1921 tggatgcgtt tgaccgcttc acccattaat tctgaacatg taacgattgt taagttttct
     1981 ggatgacgat tttcttctgt gaaaacagaa tcagtgatac aaatatcttt gattggtgca
     2041 ttgtctaaaa tacttttcgc atgatcggac aataatccat gtgacgcaca ggcatatact
     2101 tcttttgcgc cgttttcttt taatacattt gctgctcgtg caagcgttgc tcctgtgttc
     2161 aagatatcat ctaccatgat acatgtcttt ccttgaacat caccgatcac ataaccaccc
     2221 tcagttgaag catcatctgc atgatctacg atggctaatg tgctgttcaa atattctgaa
     2281 agactacgtg cacgctgaac accgctgttt tttggcgaaa caactacgat gtcatctccc
     2341 gtcagaccta attgacggta ataatgagca aacaacggca tcgtaaataa attatctaca
     2401 ggaatatcaa agaatccttg tacttgaacc gtgtgcagat ctaaagtcag tactcgagtt
     2461 gcccctgctt cttcgatcaa gttagcgatc aatttcgcag tgatcggttc atgaggtttc
     2521 gccgtgcggt cttgcctagc atacccatag taaggcaata cgacattgat cgttttcgca
     2581 cttgctcgct tcattgcatc gatcatgatc agtagttcca tatagtaatc attgacaggt
     2641 gcattcgttg attgaacgat atacacatga tcccctcgaa tactttcttc gatattgatg
     2701 gaaatctcgc catcactaaa ttgcttcacg acgcttttac ctaattctgt tccgaacact
     2761 ttcgcgattt tttcagctaa tggacggttg ccattcaaac taaaaatctt caacgtgtca
     2821 tcctgatact tattactcat gtagtcctcc ccaaaacttc tctattcttc gatagtttaa
     2881 catatttttc caagattttc tgttaatttc agcaaattct taaggaagtg aaactgttcc
     2941 gttactccaa ctggcaggaa tgactttatg ataatcgccc cagttgtcgt gtactttgta
     3001 ataacgttct cctgtttcag tttcaaaata agcgtacgct gtcacccaat gattgccata
     3061 tgtactgcca agtactttca aaataccaat catcacaggt ttgccttcac gtatacgttt
     3121 tgtagctcgc tgccatgaac ctagaagagt tgctcgtgca cgctcatgat tgccattctt
     3181 ctttaaaaaa ctagaaatcc ctgtactaac ctgaaaagga atcgtaggaa gtcctagtgg
     3241 ttggatctgg ctacgcaatg ccttcgtcaa aaccaacgaa tcagccgtat ttttcttgcg
     3301 aatctcattt ggaagcatcc accccttacg atagtcttga taataggcaa ggagaactgc
     3361 tgccgcgtaa gtaccacaaa gatagcctga gggggtagcc catttcttat acctcaaaaa
     3421 acgttcttct ggcaatccca cccagcaatt tgcgagttcc tttcctgacc ttttttcttc
     3481 gatcaaaacg ggaatatctt cttttctgtt gatccatcta gataactcga ttggctcttc
     3541 attgatcagc ttccattttt tacaagaaag accctcttga taagtatatt ttgctggaga
     3601 atattctacc ggatgtcctg attgatcgat aatcacataa ctttgatctt caaaaaccag
     3661 agctaaaaaa agcaatttac tttttcttga ctgcagctgc tgagtgctgg ctaatttttt
     3721 aattttttta tctactttga aatataaagg tttatccact acttgattca gttgggatcg
     3781 cctccttttt ttcattataa gtgaaggatt gaaaaaaaca aatcagtttg attgttcgaa
     3841 ttttcaattt tttatgttct atttgttaat tccttcattt tgtaagataa tgggcacacg
     3901 aaacaaaaaa ggagcgatcg atgaatgaac aaagaacgat tgaaacaaga tttctatgaa
     3961 gcagtcaatg aagaatggca aaaaacagca aaaatccctg cagataaacc agctacaggc
     4021 ggtttccaag atttagttga cgggatcgac caacttttga tgggagaaac agacgatctg
     4081 cttgcccatc cagaaaaagt gtcttcagaa aaaatgaaac atttccttgc atattatcaa
     4141 ctggcaaacg attatgaccg aagaaatgag ttgcatgctc aaccgcttca acctataatc
     4201 gaaaaagttg aatcattgca gtctttcgac gaattgaata agcaatttcc agaatggctg
     4261 ttggatacat taccattgcc atttaatctg gatgtagatg cagatatgaa gaatgcgcaa
     4321 acgaacgcct tctttgctta tccgccttct ttattccttc cagataagac gtattatgca
     4381 tcagaccatc ccaacggtgc acaactaata ggcgttttct ttgatatggc tgttcagtta
     4441 ttagagcttg ccggcaaaga aaaaagtcag gcagaagcaa tcacggaaca agcagtgcaa
     4501 tttgataaat tgattgctcc tcatgtaaaa agtgcagaag aaaacgcaga ttacagcaaa
     4561 atgtataatc cgcaatcttt tgatacattt attaaataca cagataaact ggacttgaaa
     4621 aaacttgtca cgggtttgat tgggacaact ccagataaag tgatcgtaac tgatccagtg
     4681 tacttcgatc atttgaatga catcctgaca gaagctcatt ttccgttatt caaaagctgg
     4741 atgatcatta aaacagtacg ttctcttagt ggttatctat cagaagaatt ccgccaagtc
     4801 agcggaatct attctcgcgt attgtctgga acagacgaag caatgcctca gaaaaaagca
     4861 gcgtattacc ttgcctctgg acaattcgat caagtagttg gcgattacta cggaaaaaaa
     4921 tattttggcg aagctgctaa aaaagacgta gaacaaatgg tgaaaaaaat gatcgccgtt
     4981 tataaaaaac gtctagaaga aaatgactgg cttggggatg caacaaaaga aaaagccgtc
     5041 ataaaattaa ataacttagg tatccaagta ggctaccccg ataagatccc agcattattc
     5101 gaccagttca aaacagtacc tgcagatcag ggtggtactt tgctttcaaa tactcttgtt
     5161 ttcagcaaac tcacattaaa agatcgtttc agcaaatgga acaaaccagt ggatcgttca
     5221 gaatgggaaa tgagtgctga cacggtcaat gcttattatc atccatttcg gaatgtgatc
     5281 gtctttccgg cagcgatcct tcaagcacca ttttacagtc tagaacaatc aagctctgct
     5341 aattatggtg gaatcggagc tgttatcgct catgagattt cccatgcatt tgataataac
     5401 ggcgctttat ttgacgaata tggcaacttg aacaactggt ggacagaaga agatcttaca
     5461 caatttcaaa acaaagcaca agcaatgatc gaagaattcg atggtctgcc ttttgctgac
     5521 ggaaaagtaa acggaaaatt aaccgtttcg gaaaatattg ctgatgcagg cggtctaagc
     5581 tgtgcattgg aagcagcaaa aacggaatca gatctttcta tcgaggaatt ctttgtcaat
     5641 tgggcaacga tttggcgtac aaaagcaaaa aaagaatacc aacaactttt actgcaaatc
     5701 gatgtccatg cccctgctaa actccgagca aacgtgcaac caaaaaacct tgacgaattc
     5761 tatgaaacat ttggtatcac ggaacaagac gctatgtatc tagcgccaga aaaaagagtg
     5821 cgaatctggt aagaggttgt gccagaagtg gtcagcttca agaaataaga aggaatttgg
     5881 gaaaatcgct tctcaaattt ttgtgacatt tcggcttatt tttcgaagaa gttacttctg
     5941 gaataccatg tattagaggt tgtgaaaaag ctgcttagtt tcaagtagta agaaaaattt
     6001 tgtgaaaatg gctttccaca tttttgcata aatttgactt actaccgaga aactggcttt
     6061 tgaacaccgt ttatcgagag gttgtgaaat cagcgtcctg acctgagtat tagagaaaaa
     6121 aacggaaaaa tagttcctca tattttgccg tttgttgagc taataccgag ggtcagctga
     6181 ttgaacaccg tttagtggag agattgtgaa aaagctgtcc tgcatcaagt aataagaaca
     6241 gccaatcaaa agcagcaagg agaacctcat ctataaaaag atagaaggaa gtgagacatc
     6301 tattggtctc atttccttct atttttactt tcctcagtca attgttttac atcataaaag
     6361 gagaaattcc acttattttt ttcgctgttt catgacataa acaactacac cgattaacaa
     6421 aattcctcca gctaatgcta gcaacgggtt attaaattct cccgtttttg ggagtccttg
     6481 agtgccattt ttttcacttt cttttgctgt cctatccgga tgagaaggtt ttccatttgg
     6541 ttttccattt ggtgattcat ttcctggatt ttctctttct ggtttgtcat ttcccggggg
     6601 cacatcggca gtttctcgca cagtgatcgc tttttgctgg gtaacagatt ctgtatgagc
     6661 agaccatttt tccccatcaa atatttccga acgataagtg acttctagcg tatattctcc
     6721 ttttgaaggt gctgaaaaaa tagcggtatg attagaaaat tgagcacttg actgtgtttc
     6781 aacgatttgg atcttttctg ggaaataacg catatcttca aagacaggcg ttccttgatt
     6841 tggtgtgaaa gttgacacaa aaactgtaaa aggcttcagt tcaatgatat ttccttctgc
     6901 attctccgcc acaccatttg cttcttgtat aataatcgga ttattttgtt catttggttt
     6961 tgtctgattt ggttcttgta cgattgcata ctctggatat tccgaagacg gtgaggtttc
     7021 tccgtcaaaa atagcaccta cagataatgg aagaccatcc actctttcct tttctgtttg
     7081 acgattcccc ggtttaatct gcatcagtcc atgaacatgg atacttccat tttcatagcg
     7141 cgtaacaggc tgctgacctt ctttcgaaca tgtatttaga gactcgatcc aatcttttga
     7201 tatggtatgt ccaagagtat tttctgattg tttctctcgt tcattccaat agtagttcaa
     7261 cgagtggtag atttgatctt cgttttgatt caatgggcgg atctgctctc tcatctcaag
     7321 attctctgtt cggaaagata agataccaga agcatggtag tctgtgtttc ctgctgaaga
     7381 ggtataaaga gccacattat atgaaccgtt attaaaactt gtactgctat tgacgataat
     7441 atctgttccg ctattggaat cgattccttt cgcaagattt tcgaaagaca aactattgat
     7501 caattcatga gggcccgtta aagaagaacc gcccattttg aatccattac cgttaccttc
     7561 tccctcaatt cctggtaccc agccgtttcg ataagcaact gagtttttga tgattacttt
     7621 tccgatcgaa cctgtttcat ttttagcaaa aaggtcccaa ccgtcatctg cattatgata
     7681 cgcgatacaa ccgtcaaata catttccttc tcctattgtc agtttggccg caaatccatc
     7741 ggcatcttca aatcctggat cggcattttc aaaagatgta cagttcttga ctaagttatg
     7801 cgcgggccat gcttcgaaag gatcatttga gctaccgctg atttggattc ctgtatttcc
     7861 attccgataa gcatgaatct cttcgatcaa attatgattt ccagaaatct gtaatccttt
     7921 ttgcatagct gcactatttg taatgtcaaa ccctcgtaaa ccccagtagt gactgaacag
     7981 tgtcatccca tttccagttc ctttaaaatc aagtacagga cgcttgtttt ctggattttt
     8041 ctctgcaatc aactgaatcg ggttttctct tgtcccgtta acgccttttg gaatcgtcaa
     8101 tcctctttcg aaggaataag ttccaggcgc catgacgatt gtctgcccag catatgcgta
     8161 tctcaatgct tgtgtcagag ataatggatt ttgtctgctt ccgtttcctg catctgaccc
     8221 ttctggtgtt acataaactg tcgaatattt aggtttacgg tagtccactg tatgaagaat
     8281 ctcttttgtt tcataactag tcatctctac gtattcttct ggcggatagt ttttatctgg
     8341 cgtaaaactg aaagtaaatt cgttcgttcc ttccatcagt tttgtctgat aatcaaattc
     8401 ccgattcgca gtgatagcca agttttttgc gttcttaagc acctctcctg ttgctttatt
     8461 tttaattgtc aataatccgt cagcatttgc tctaaaggac atagtatgat cttggtttcc
     8521 tgaatgatta ctagaggtca cagtgtaaat cggatcaatc aagcgagtag gtctttcttg
     8581 cccaggagga tctgtcgcag gatctgagta agcaacttcg attccttcta ccttgatcgt
     8641 catattcctt gctgcagcaa atcctgcata aataaccgat tcatctgaag cgtataattt
     8701 ttcccagtca tacatgatgt cagaagccat ttcttctcct gtttcaggat cgtaataata
     8761 tgcctctaaa ccagtattcg tttttttcat ctctacgtcc agtttagtaa tcgagttcgt
     8821 ggccggaaac agattcccat gaccgtttcc gaatatatta taagaacctg tttctttttc
     8881 caagaaccct gctgaagtct ctaaaggtgt tgtttccact gagacactcc caggagctgg
     8941 cgcagcttgc ggatcttcag atgatattcc tgtgattgcc cgtgtcccca atccaagtcg
     9001 catcgtatat ttgctgccgc tcgtgttgac cacttcttgt ctatccgagt cccagacata
     9061 ttcgatccga ctgcctagta tggcaaatga atttgaataa aatgcctgcc caaacaaatg
     9121 attttttggt actgcgtctc ttaccattaa agcaaatcct tcttgggcat tcgtataggt
     9181 ccaagattct atttcgatcg tcgcccgcat cctaaagttg atgtctttgg ggattgcttg
     9241 ataataatag gtcattccat ctgatccgct tgactggaat tttccgccgt tattcgtgga
     9301 agagagccgc acgccgtctt ctaatggttc aatggcatac ctactagcac ttgtgctttc
     9361 tccgaaaaca acccctttcc actcttcatt tgcttcttca gattgctttt ttgtcttttc
     9421 attttctgat tctttcatca taggatcatt tccttcttcc gtatccatat cttcactagt
     9481 tcttggtatt tcctccattg ttgacatccc ttcaatgttc acgtgtgcat tttcttcgtt
     9541 cacagactgt tgttcgttag cagcaacttc aacgacagaa attaaaaccg gggtactgag
     9601 cataaggaga ttcatcatta gcgcactgta ttttatacgt ttttttaaca agtgtattcc
     9661 tcccgtaaca aattagaaag cgtttactta aagtattacc taatttattt ttactgtcaa
     9721 taaaaaacaa aattataata agtgtatttt ataaaaagaa aaaatataac ttaagttgtt
     9781 gataataaag agatttatta acggaaaaag aataagtttt tttaaaataa cgtataaaaa
     9841 agagcataaa acaaagtcgt ttgttttatg ctcttctttt attattttgt gctttttatt
     9901 ttatcaaatc actttgtttt tttataaaat ttctgcgcct agtgtggact caaaatgtct
     9961 caatgcccat tcatgtccaa cgggatcgaa acttgccacg gaatctttga aaatatggag
    10021 tttgtatcct agattatacg catcgactgc tgtatgcaac acacagatat ctgtacatac
    10081 gccggtcaaa taaatatctg tgatctggcg ttctcgcaaa cggatgtcta gatctgttcc
    10141 actaaaagcg gaataatgcc gtttgtctat ccaataaaca ttttcctctt gctcatagtc
    10201 ttggtataaa ctttttaaag agccgtacaa ttcccgtcca cccgttccta tcacattatg
    10261 cggaggaaat aagcggttct ccggatgaaa ggaatctttt ggatcatgcg catcaatggc
    10321 aaaaacgaca aaatcctttt gatcaataaa cttctttgta tgagcagtca gctttttttc
    10381 aattacttgt cctgctgtcc cagtagtcag ctttccatcc tctgcaacaa aatcatatgt
    10441 gtagtcaatc gagatcaatg cgttcatctt agtcctcctt atacgagatg tttgacaaat
    10501 tcgatcacca tttctgctga tcgtttgcct gcgtcttcga taaattcgtc aaagctttgt
    10561 gtcgccgagt gatctgctgt gtcgctcatt gcacgtacga tcaaaaatgg gatattgaac
    10621 tgacgagctg tttgtccaac agcggctcct tccatttcgc aagctaatgc ttcagggaaa
    10681 ttcgttaaga tttctttgat tttatctggc gagtcgacaa atgtatcacc tgtaacgatc
    10741 aatccttctt ttgcgttcaa attcgttgct tttgctgctt taaccatttc actgcgcaaa
    10801 tatgtgcttg cttcataata tagaggcatt cctggtaatt gtcctgggtt gtagccaaat
    10861 cctgttgcat ctgcatcaaa ataagctact ttgtctgata taacgatgtc acctacttgt
    10921 aagccttcac cgattccgcc agctgaacct gtattgatca ccatgttcac gccgtattgt
    10981 tggatcaaca gacttgtggt aatagatgct aagacttttc ctataccgga acgtacgacg
    11041 atcacttcgt ggttacctaa tgaacctgaa ataaataaag cgcctgcgcg ttcccatgat
    11101 aatggttcgc ttaggttctc tctcaaaatt tttacttctt cttccattgc gccgataata
    11161 ccgattttca tctttttaat tccttctttc tatataaatg cgattgctaa aaaaaccaca
    11221 cataacatta ggataacaat aataagagat ttcatcaaaa aagagttcca tttggtcgtc
    11281 ttttcccgtt cgctgatcct tgttttcgtg attggtttat tctttttgct ttctttacga
    11341 taaaagctgg caatcttttt ttcttcttgc tgataagctg tttctgcttt tcgctgtttt
    11401 tttaatgact cgcttgcttg tgcttgttgt cgcttgcgaa gttcgcttcg cgtaataagc
    11461 ggtcctttgg ccataagccg atccctctac tttcctttgg tcaatagttc ccagtattga
    11521 atcgcataaa tcgtctttgc atcacaaatc aactgatctt tcatcgcttg ttgcgcctct
    11581 tcaagagtta gatgatacaa ctccagtact tcgtcttcat cttgtgctaa aggattctcg
    11641 accttctcta ccccttgtgc atgataaata tgaaggactt catttgcaaa acctggtgaa
    11701 agatacatcg aagtaagatg gctcagcgac ttcgcacgat agccagtttc ttcttctaat
    11761 tctctagctg ctgtcatttc gggattttgt ccttctcctg gatcgatttt tccagctgga
    11821 atctccaaaa tcactttttc taaaggtttt cgaaattgtt tgacaagaag cagacgatct
    11881 tgttcgtcaa aagcaatgat cccgacaccg ccaggatgaa ataccagttc tctcttcgaa
    11941 gttcctccat ctggcaaacg aacttcgtct acagcaacat caataatttt tccgctatag
    12001 atttctttgc gactgattgt tttttcttca aattgctcat tgttcagcat atcttctcct
    12061 gctttctttg taatcataac tgcttttttt tcatttttca atttttctca aaaactcctt
    12121 attagtttac catagataaa ccttagattt tctctaaata tagaaaatcc ttctttagtc
    12181 cgatactctt tctagtagtc cgtatgctaa aataagtatg taatcgaata acgaaaagaa
    12241 ttcaatactc aacgataaat gacatggagg gtttattatg ataaaacgaa attggccgtg
    12301 gcttctagca ttggtcttgc tatttctctt tgtccgaaac aatagttttt cttttgttct
    12361 aggactttta ttgataggtg tagttttagt tttgtgggga ctctttggtt cgcgcaggaa
    12421 aaagggaaaa caagaagaaa ttcctgcact gactgacgaa cttgaaactc actatgaaaa
    12481 aagcggtatg acagcgaatg aaatttcatt ttttcgacaa acaatgaatc aaacaaaaaa
    12541 tgagatcaat caattgcaaa tgaatatgca gcagacagcc aaacttaaag caatcgatct
    12601 ccgccatgat ccagtcaaag cagcgaaagc tttattcaaa gaattggtga aagaaccaaa
    12661 ccgactgcat gaagcaagtc agtttcttta tacgcatctg ccgaatctag ttgatttgac
    12721 aaataagtat aatgagatca atgaccatga gatcaaaaac aaacagacct atgaaaaact
    12781 agaagaaagc gcgcagatca tcgaccagct gtcagcttta atcgcacaag attatcaaaa
    12841 attcgttgct gaggatctag atgatatcga cgtagagata tctgttgcga aacagagctt
    12901 aaagcgtgat aataaatatg aaccagaaca aaaggattaa ctcttttgtt cataaaagaa
    12961 gactcggaac attcagatga tgtctggctg tttcgagtac tttttctaaa gtagagacat
    13021 tcatgtaaga atcgagaaca gattcaacaa ggaggaattt tttatggatc ataaaccgac
    13081 tgaaacaaaa gatgttacac cagttaacga tacacttgaa gatttattga acaacccgtt
    13141 ttcaactcca acagatacgc tgaccacttc acaacaagcg gagattgatc agctccaaga
    13201 gcaacagaca gctgctcgtt taattgacaa actaccggcg gaacgccaag aacaagctaa
    13261 acagctagca gcaaaaatcg atgctaatga tgcacagtct gtcatcagct acggatcggc
    13321 tgcccaagca aaattaagtg aattctcaca atcgatgttg aatcatgtgc aagcacaaga
    13381 tattggtcct gtaggagatt ccttaactga actgatgtat cgtctccaag aagccaatcc
    13441 tgacgaactg cgtgccggag aagggaacat cttccagcgt gtcttcggga aggtcaaaca
    13501 atcgatctat gaagtgacag caaaatatca aaagattggt gcacaaattg acaagatttc
    13561 agtgaaatta gataaagaaa aagacggtct gctaaaagat aatctgatgt tagaacagct
    13621 ttaccagaaa aacaaagact actttgatgc actgaatatt tatatcgctg ctggagaatt
    13681 gaaaatggaa gaacttcaaa caacgatcat tccagaagcg atgaaaagag cagaagaaac
    13741 tggcgatcag atggatgttc agattgccaa tgattacacg caattcctag atcgtctgga
    13801 taaacgtacg catgatcttc gcttagctag acagatcacg atccagcaag caccacaaat
    13861 ccgtttgatc caaaacacaa atcaggcctt ggctgaaaag atccaagcat ctatcgctac
    13921 agccattccg ctgtggaaaa accaagtcgt gatcgcattg accttgcttc gtcaaaaaga
    13981 tgcagtaact gctcaacgcc aagtctctga aacaacaaac gacttattga agaaaaattc
    14041 agaaatgctg aaaatctcag ccatcgaaac agcgaaagaa aatgaacgtg gaatcgtgga
    14101 tatcgagacc cttcaaacca ctcaaaatga tttgatcgaa acaatccaag agacattgcg
    14161 tatccaaaaa gaaggaaaag aaaaacgtcg tcatgcagaa gtagagcttg gacacatgga
    14221 agaagatctg aaacaaaaac tgttggattt aacccaatag atcacctttc gcttgttact
    14281 aaaacaaacg cccaacaaca gtttattaca actgttgatg ggcgtttttc ttatctttct
    14341 tctaacttct ccattggagt ctatttctca ttcatcttgc tgtgtccata gccatagcca
    14401 aaataaatga tcagtccaat aaccattacg ataccgaaca cgatccatgt cacagtttgc
    14461 agacgaagca tcaatacaat acaagtcaag atggagacga ttggcaatag cggaacgaat
    14521 ggcactttga attctccagc ttttggtttg ccaaacattt ttctcaactt cagtaagcct
    14581 agactaacaa gcatcaaata ggcgagcgcc atgatattcg ttagttccgc taatacattc
    14641 aatgggaaaa atccggcaaa gaatacagct gccacaccag cggcataagt cgcttttttc
    14701 ggtgtacgat gcttttgatc gatttctgtc ataaacttag gaagtaagcc atccttgctg
    14761 acagcaaaca gtaaacgagc aagtgaatac atcatcgaga tcgttaccgt cagtagcgtt
    14821 aaaattgccc ctacagcgat caccgcgccc actgttccat gtcctacata cctcattgca
    14881 aaagccactg ggtcattgac tcctagttca gtatacggaa cgattcctgt caagatcagc
    14941 gtcacgatga tgtaaaggac agtagcaatc agtatcgaac caatgattcc tcttggcaca
    15001 tctttttgcg ggtttttgac ttcctcggct gccattgcaa cggcatcaaa tcccaagaaa
    15061 gcaaaaaaca caagagctgc accatctagc acaccggaaa aaccaaaagg catgaatggc
    15121 tgccagttat caggtttaac gaaaaatatt cctacaatga taaacaacgc gatgatacca
    15181 aatttgacat ataccatggc attattcaaa cgtaatgctt tcttggcttc aagcgacacg
    15241 atgtaagtga cgaatatcac tacgagcacc gcaatcaagt caatgtatgt accattttcc
    15301 ggattgtacc cgccagttaa tgcttgtgga agttcggccc ctaaactttt caaaaaacct
    15361 tgcatatagc cagaccatcc ggaagcaaca gaggaaaccg ctagtaaaaa ttccccgatc
    15421 aacaaccacc ctgtcagcca ggcagcaaat tcaccaaata caacatacag ataggcatat
    15481 ggcccaccaa tcactggcac tcgcgaagca aattctgcga aacttagtcc agacaaaatg
    15541 acaacgattg cagctaagac aaatgaaagt gacagtgctg gaccagcatt ttgattggcc
    15601 gcaacccctg tcaccacaaa tattcccgta ccgatgatcg caccaattcc aagcatgatc
    15661 aaatcagacg tttttagttc ttttttcatc tcactcgtat gattgagatt gatctctttc
    15721 ttacgaaaca aatccattga agttcctccc cattttaggt tggaaaacag cgattaaact
    15781 gttttcatat accctaaaag gataccacct aaaatgacta aaaggcaacc aacaataaca
    15841 taaatgagct cttttttcgt ttttctttcg ccaaggatga aaatcccacc taaagtagag
    15901 ataatgatcc ccatttgtga aagtgagaag ccaacagcta aaccaagtga tttgactgtt
    15961 agaagcatgc agatgtttcc taagccccat aataatccac agatcatgtt tttccacaca
    16021 aactgatcga ctttaacttt acggaaagca aagaagcttg cgcctaagat cattccgata
    16081 ctttgaggta agataattga catcgcatcc aaattcgccc agttgacgat gatggtatat
    16141 acaccataac caatcgtaga gaagatcaat gctctaaaac ccttaccaaa atcaagcatt
    16201 ttattatctg tatctatttt tccttcgtca tcttgtcggg cagtcaaata cgcaccgctt
    16261 acaagaagag ctaaagcaat aataccgtat acataatctt tcgtttgtgt ccattcatgg
    16321 aagaaaacgg ctcccgcaat cgtattcaaa attaattgaa aaccagtaga caatggtagc
    16381 cctacagaaa cacccatgta cttcattcca tggaactgtc cattttgtcc aacactccaa
    16441 gctaagccgg ataaaaatcc gatcaagaaa atccaagggg tgattacagg actaacaacg
    16501 aaaaagacaa ctaaagaaaa cagaaatgca ccgatcgtca ttcctagtgt ttgttgattg
    16561 gcatcgccac caattttacc acttactaaa ccaatactac cccatgccaa catagggact
    16621 aatgctatta aaatttccat aaccaatctc cttttatttc acatgataag aaacttatca
    16681 gcgaaaacgt taacaacaca agcacttttt ttcgtttcta acgattttct aacgtttttc
    16741 attataaaaa tttaagattg ggaaaaataa agtcattgtt gtcccttttc cttcagttga
    16801 ttcagccgtc aaatggatac ctaatttttc ggccagattt tttgctaaat aaagtcctaa
    16861 tcctgttgaa tgtgttttgc taagtcgacc atttctccca gtaaatccct tatcaaagat
    16921 ccgtttcaag tcttccgctg ggataccgat ccctgaatct ttaactgaaa ggctgactcc
    16981 ttgcgcttcc ttttctatgc tcacaatgat gtttccgtga tccggcgtat atttgatggc
    17041 attactaagc aattggttga agataaaagc gatccattta gcatcggtca aaacagtatg
    17101 atcctcacca acgatctcgt agcgaatatt tttttggata aaataatttg cctgactgcg
    17161 tatcacaggc tgaatgattc cttttagtcc atattcttgg atgaggtagt cccgcgagaa
    17221 actgtcgagt cttgcataat acaaaacttg ttctacgtat tcatcgattt ttgataattc
    17281 attttctagt aatgtatact tttgatcgtc aatatcgaac tcgatcgaac gaactagcaa
    17341 tttagaagcg gaaagtggca ctttgatttc atggacccat gagtcaatat aatctttttg
    17401 gtcttgttga ttactgattg ctgattgcat tacttcttgg tgttcacgga tcagctgatt
    17461 gatatattct tctaccagtt tttcttcttc tgtcctccca ccactcaaat aattttgcaa
    17521 aggggaagtt ttttgtatgt cggctaattt tccccaccat cttctcttgc tcaagtaacc
    17581 acccagcaga taaaaaatca gaaagacgcc ctcaatcaaa gctaaataag caattgtgct
    17641 caaatcaact ttgatgtttg gcgccagcca catcacaaag atcgtcaatg caataaaaaa
    17701 cagccatccc aagatcaatg tccactgatc tttcaaatat ttaaaaaaat tcatcttaac
    17761 cgctcatcct ttcagggaac gatatatccc tgtcctactt ttgtttcgat atatccttgg
    17821 atacctgcct gttcgatttt tcggcgtaac cgattgatgt tgactgtcaa tgtattgtca
    17881 tctacaaatc gttcgtcatc ccacagtgct cgcagcaatt tttcccgact taagatcttt
    17941 ccattgtgct tcatcaaaat aaataataga cgatattcat ttttgctcaa atcaatgatc
    18001 tctccgttga tttccatact tccgttgtca acattcaacg taatgccatt gtggctcatc
    18061 atttcactag ataattctgt gtagttgtaa gaacggcgaa gtaatgcatt gatttttgca
    18121 atcagtacat cgatttggaa atgttttgtc acaaaatcat ctgcccccat gttcatagcc
    18181 atgatcatat ccatattcgt attcctgctg gagataaaaa tgatcggtac tttcgatact
    18241 tcacgtattt tttgacacca ataatagcca tccaaaacgg gaagattaat atctagtaat
    18301 acaagctgtg gttcttctct ttcaaagtct gagaacactt gattgaaatc ggtcgttcca
    18361 aaggtttcaa attgccattt ctcaagttcc tccatgatca attgtcgaat gatctcttcg
    18421 tcttcgacta ccatgatttt tgccataaaa aatccctcca tctttcttct tgctactaca
    18481 atatcaaaga agcccttgtg aaacaagttg gcttacctgg aaaatgtcat tttagcaaac
    18541 gttaaaaaaa actggaaata tgcgatttaa tcgcgtttgt ctcttccaag cagcttaaat
    18601 ttattatgtt attcttaata attggtctat tatctgttat ttttctttcg ttgtttttgt
    18661 cttttaaatt ttcgataaca ccaataaccg aaaagcgtta gcactagcaa tatacctaga
    18721 aggagtataa gtaatcgata aagatggtat ttttttgtct gttctatctt tgaagagact
    18781 tcttctgtaa agggaactcg tcttcctgtc actagtaatc ggtgagtgtt cacggtgtaa
    18841 ggtgtacaag tgataagtgt gactagatcc tgcttttctc taattgctaa tgtatttgtt
    18901 tttgttggaa ggacaacaat tttctcaacg acttcataag cgagcatccg accatacact
    18961 tcaagataga atcggtcatc ttgctccatt tcttcaagat ccgtaaagag ttttttctca
    19021 gcaataccgc tatgggcggt aatcacggaa tgtgtactct ctcctccagt tggaaaagag
    19081 gtgccttgca ggactgtcgc tcctctatct aacaaaagat cattcgtttc atcaaataaa
    19141 ggcaagctaa cattgatttt cggaatatag atggctccga tcatatgttg ttcgtaatat
    19201 tcttttcctg gattttggac atttctaacc gcttgctcaa aaggatcttt gacttgtccc
    19261 attcctggga ttactgatgt atgagcaagc gtctgatttt tcttttcatg cactgctaat
    19321 tgttgctcat gtttttcttc tttatcgcgt tcaagctgct tttgataatt ctcgatcctt
    19381 ctttgatcca aataattgct gagagcatct gctaaaaatg gatatgtaaa aactgcagct
    19441 ccggcaagaa acatcactgc cataaataat tgtaataaaa gactgatttg ggtttgaggt
    19501 ctattaccta ttttcttttt cattttcttc accttcttat ttaaaaaaac tgcagcaacc
    19561 tttataaaac cgattgctgc agcttatttt tgttgcttat acttcagact tcattttaga
    19621 tttacggtac cagctgtacg ctccaatcat caaacttatt ccgattagta agaatgccaa
    19681 gagcccgttc ccaccagtag caggaagtga cccgcgtttc atattcggta ctctttctgg
    19741 ccctggattt tctactccta cgatctgaat ttcttcatta tagcttccat aagagatgat
    19801 aaattcagta aaggctgcct cacctgttgg aagtacataa ccttctggcg ccatggtctc
    19861 tttcatttga taggttccat aatctaaccc ttgaacctca aagtatccat ttccatctgc
    19921 gacaaattct gttgcttgtc cgcttgttgc ccactcaatt ctatctgctc ctgattcata
    19981 aacagctaaa ggcgactccc ctttataaaa tattgcgtat tcagaacggt cttcattcca
    20041 caattcgaat cttgcatctt taagcaattc actgcttgat gcatcatatt tttcaaactt
    20101 tcttccacct gttgtgactt tctctggcgg ctctactcct tcttcataat caaattcatc
    20161 atttcgtccg atttcaataa ccgctgtgtt gttatggaaa tcatccgctt ctagttctgt
    20221 ggaaaccttc aaattatacg tgatcgttaa atctcctcca cgtgcatctg ctaatctacc
    20281 taaagtatct actttggcta ctccattttc ttctgtcaaa atcgtcacga caaacccgtt
    20341 gcttactact tctactatat aatcttgtcc ttttgttaaa atcgtctcgc cggctcgcac
    20401 ttctatttcc tgatcataat aagttagacc aactgtaggc gtatctgtga ttttgtaatt
    20461 tttgataaca gcgcttccgt tttcaaggac agaatcgatg aaataaggaa taggtgctgt
    20521 gatcgtgtaa gaagccgttt ttcctctttc tagatcaata taagaaatcg tttcttgttc
    20581 tccagcttca ttaataatcg ttacttgtct aaggtcgctt tcctctacat tcatctgttt
    20641 atctgcttct ctgatttcgt ttttcggata caaatgcaca tctgtattgt atgtgtcttt
    20701 ccatgtagaa ccatcccaca cgccatccac catatcaggc atcatcactg gcatcataac
    20761 aaccattgga gacgcgattc tggaaatatg agctggcgaa taagtttcta agaaaagata
    20821 gactttatct ctagaagaag gtgcttcttc cgtcacttgg acggtagaga ataccgcttc
    20881 tccagcttca ttcgttgttt gtttgtcaat acttgtcagt cgatatttta aaaaccaact
    20941 ttctgtgtgc cgtttttgaa tccattcaat cgcttcggca gaattaagac cgtcttctcg
    21001 ttccggtgtt aaaggattat ctcgaatcaa atcgtaatat acttccgtta cgtcaaacaa
    21061 atcaaaatcg actcctggaa tattttcttc accaaaatcc gtagaaacca atccgctatt
    21121 cggtcgttct tccggaacag atgagaaacc ttttttgtgt aatgtaatcg tcacttcact
    21181 tggtttttgt gcgacttctt cggctttgac tagttttccg ttcccgaaaa tccctattcc
    21241 cgcagtaacg agtaacagta cactcattcc cgtgacaatc atttttttat tcattttttt
    21301 ctccccgttc tttttttatt aataaaacag cacctaacaa taaaagaatt cctagtaaag
    21361 aaatcaacgt tcttgcttca ttcgtttgcg ggaatacgga ccattttcct gattgacctg
    21421 gattgcttgg accactcggc tctttcggtt tgtccgttgt ttcttttacg gtctcgttat
    21481 aaacacgtgg tgttgcggat tcataggcag agactggaac tttcccggat tctaattcag
    21541 ccatctcctg tccgttaact actacatatt gtggttggcc ttttgcatcc acccaaaatt
    21601 cagggatgat gacctcgatt ctcttacttg cttcatccat ctcgtaacct tccacacctt
    21661 ttaattcttc aaaataatag gtgccagaag gcaagaaacg ttgtcctgtc gtaacaaggc
    21721 cctctttatc cgaaatgaat ttgcttacat ttttgtcatt caaaggatca ttgtctgctg
    21781 gtttgatcca ttgatttttc aagtcattag caggagccat atctaaataa tatttccccc
    21841 cttctatcat ctgatataga gcaaaaacag caccttctaa tggttttcct ttttcagttg
    21901 ttcctctttg acgtccatac ttgaagaagt atggatctct tagataaccg acatttttag
    21961 gatagatatg aatctcttgc agttcctcgg ttgattctgt cgggtgataa attggcaaaa
    22021 tagcagcaat tgggatagct gtctgatcga tatcaacatc caatccaact tctggatcaa
    22081 tcgattcttc gaaaatcaaa taaactgcat cccgatcttg gtttttctta ggtgctttga
    22141 tacgtgcaat cccgtctcct aatccgccaa ccgtatcttt cccagttgtt accgaagtcc
    22201 ctatttggac aagcctttct tctgtgatta gctgacggat attgtttctg ttcgtttcag
    22261 cgatttgctg gacgaattct ttaggagtca tgttttcagc agcttttttt tcatgataat
    22321 aatcggtcat gtcgtaaatg gcaaatgtcg ctccatttag tggcaccgtc tgtgaaatga
    22381 tatctgctgt ttcccctgct tcctcagaga tagccaagcc atcattttca tggtattcaa
    22441 attgatcgtt ataatcgata tctcgaatca tccgtttatg caacacgaat tccacgacat
    22501 cttcccctga ttcttctgcc gacatcgcat gtggaagaag gactgctgca agcggtagta
    22561 aaacaagtaa ttgaagcatc aaacacaaca gctttttcat taccttgctc cctccttttt
    22621 agattgtctg acaaacaaac cataggctgc tcctgcaagc gccatgaaga atgcagcaag
    22681 caggatatag aataatcgtc cggatcctcc tgtagcaggc aacggatttt caggatcgtt
    22741 cccgacacta agctgtaatc tgattcgatt tgtctccaaa agttcatatt ctaactcttg
    22801 ctgtagatca ggcgtctctg cttcttcgtt tggataatac gaaatctcaa cttttagtgt
    22861 acccgcttct tttcctggaa ctacccgatc attttctatg tcaggttctt ctcgataact
    22921 aatttctaaa ataaagtaac ctggaagttt tctatagctg tcaggtgact tcacttcctt
    22981 gatccgatag accccttcca acaagtctga agcattgaat agggaagaat cgtttggatc
    23041 tggtgccaag cctccctcga tgatttcata ctctcctgca ctattttgtt tctcaattga
    23101 aaattctgcg ccttcaagac gtcggctatt catactgtca attttcaaaa gttccaaatc
    23161 gattggcgct agttcatctt ctaaggttac ttgtagtaca ccatcgatga tttcatagcc
    23221 ttccggcaag ggagcagttt cgtcttcgat gaccgtatct tcaatgataa acttaccttc
    23281 ttcattcagt gcaaactgga acacttgatc agatagccga tacccatttg gtgcgacaga
    23341 ttccgagacc tcatattcac caatagacag atgcagaggt tggtcgtaat ttaaatcgac
    23401 atacttttct actcctacgg cacgagaata gaagtatccg atcccaggca aacgtttcga
    23461 ttcttctggt tctaacggat taccagtgta tgctcgatac agcgggaagg aagaatcccc
    23521 cactttcctg atatcgaaac ctgcaccatc taatgtatgg ccttcatggt ctaattttct
    23581 aactcggatc tcccgataat caaacgtatt gtaagcagtg aatgctaccc ataaacgatc
    23641 ctcttcatct gttgtatacg tgagatattg ccaattttct tcacctgttt gcttgagttg
    23701 gatagtccct atagcattat cgtaaagtcg caagatccat gtgtcatttt catcaggttc
    23761 tttgaaacct tctgggttct ggctctcaat gattttgtat tctgcatagt tggtagtttc
    23821 ttcactatcc ttataaacaa atgaatctgg ctctccgatt ggatgaataa ctttctcacc
    23881 atccacgatt acatatttcc cgtcttcatc tactgaatag aacgtgagtc tccctttctc
    23941 ttcctctact tcgttcacac cagaaagccc tttcgctact ggatatccga tttccagtcc
    24001 gttaccatct gtttcataaa gttcaaactc tgcgccattg attggcatat tactgccttc
    24061 aattagttta ttgatattca tgtggattgg cttgaattca tttcgcattt ccagtttgac
    24121 atacaactca tcatactctg tgccttctat gttaggctct tgtacaacag aaagcttgtg
    24181 atattctcct tcataatagg aattcggatc tttaccttcc ggtccgccga ctctcgaacc
    24241 agatgtatat tcatctacaa gctcgtagtg acctaacatc tcatcttcgt tggctgcata
    24301 ttttttgatt tggaattgat acgtctcatc tactaactga taacgtggtg gtccagcgat
    24361 ctctctgatc caaaatatgt cgctgactgc tccagtttca tctcctaaaa tcggcatctt
    24421 cgtttcaaag atcgctattc cgtccgtggt agttttactt gtttcgattg gttcggcttc
    24481 cccactctct ggattttggt catataatgc aaattcgact ccagccaacg gctcgccatt
    24541 ttgacccttc ttgctgactt ggaaattacg gcctttatgt tcgtaacgaa tggaagaaga
    24601 tgggaaatac atccgtccag tagtgatatc attctcataa tcctccaggt tctgaatata
    24661 ggccatattg ttcactgtgc aaaaatttcc gtcttgagct cccgcatcaa gttttacttg
    24721 gtacttgatc ctgaccattt ccccttcacc taaagaaatc ggagaaaatt tataagcttg
    24781 gttttcccgt ccgacatacg ttgtcggagt cgccactact tctggtgggg cttgtccatg
    24841 acgttcttca tcccatattt ctggctcttg gtacttcatg ccgttttctc ctggatctag
    24901 atggtaaaac tgaatatctg ccgatctgcc agctccaaat ggtgagacat taaaaccagt
    24961 ttcatcataa agtgtggctt gctggatcgt tctcttgaag gtagaagaga tatcctccaa
    25021 ggctgtaaca atgtcattct tgttgttcgc attgtaatat cgcggtgtta cgccatcttc
    25081 ttctaaatca gcaatatttt tcaatacatt tcgtccaata gcatctagtc gttgtctccc
    25141 gctaacgttt ccatcgattc ctaaaccaat ggagaagata cttggcgggt tctcttgata
    25201 cttgtttttc agccaatgat gataagcaac tgtgttgatc gcagatgact tttcttgcga
    25261 agtaggactg ccattcgtat tgttattaga agaaccatca ccaacccagt gagtcgcttt
    25321 attatctcct tcttttacct ctccataacg atagtgtaca tttacttcat cttctggacg
    25381 gttaaagtca gatgcatagg taactggatg agtaaaccct gcattagaag tattcccaga
    25441 aaactcctca aaatttctga acaaaacgtc attatcttca gcaatcttat cttccatcac
    25501 agtccagttg ctccaagctc cattagtgct cgtacgataa cttgactgaa tcggtgcata
    25561 actgaaggtc ggtgtcccat ctcccacaac gattaggatt ttttctggat ttctattttc
    25621 tccgccattg tctttataca atgtttcata gccattttta agccctagtg taagcggagt
    25681 tcctccgatt ggtgtacgat ttaatgctgt attctcactt cccgtcaaac gattgatatc
    25741 atttgtcaat ggaaagatgt ccgaactgat ttgctcatgg ttattgatag ctgtactatt
    25801 atggtaaaaa ttcaccatac cgattcgaat atccagactc gggtcagaga gcagatcttt
    25861 actcatttcg tttactgcct caatcaacgc agcattttta gtttgactat tggtcccagc
    25921 ggttaattca ctcatactcg ctgacttatc tagaacaaaa acgatatcga ttggactgat
    25981 ttcctttttc tcgcccccga tgacatctag atagacattg taaagggttg ttgcatcttc
    26041 aggatgtaga tcagatgctg tgctgcttgg cagaacaggt tctacccatt ttttgatgta
    26101 cgccccgtta taatccaagt aactgttttc aaaattccgt tcatttcccc aaagctgaac
    26161 ggtttgagct ggaacgtttc tatcttcttc gcctgcagtt gatccattat tagtagcata
    26221 gtcgtaattt cgccagttcg ctcccccgtt tgtataatca ctatcatcga tcaagttctc
    26281 tccgtcatat ggcgagcctt tgtctccttg atagcgttca ttgctttgtg tccaatttcc
    26341 taacactttt gtatagtcat tcgttgtaaa cttcgggtag cgatgctgtt cgttttcaac
    26401 ttctccgcta ggatagatat attcataggg gtcttgcgta gctgttggcc caagacgctc
    26461 caaaataccg ttgaataact ggcgttgaat gcgctggagt gcttgaccgc tgggtacgat
    26521 tggttcgtcc agcttttcca tctcattttc ttcgctacgt tgaatttcag cggaatcttc
    26581 tgtatcagtt tcattttctt gtaattcttg gttatctatt ctttcatcaa ccaccacttg
    26641 aagaggtagt tacccttcgt tcacatcggg gatcttttct gtttcaaaaa tcaatgtgcc
    26701 ggtttctgct tcttttgaat aaacttttcc gacataccat tcttgaccat cgatcgtctc
    26761 gacttttagg tcttctccat cttcatcata ggaggataga cctgttccac gaacgttttt
    26821 tacggtacca atcccggcgg ctgccgcatc gattgctaaa cgtattctac cttcattggc
    26881 actttccttt ttttcaaaag ccagatgcca ttcaagggtt tcttcttttt caatatactt
    26941 cccttcaatt tttgcatagt cctcatctaa gaatacctca tcctcctttg tttctgttga
    27001 ctcagcagct acatccaggg tcgtcatatg atagactgac tggaaatatg gtccaaatag
    27061 caaactgaca gttactaact gcaaaagctt caattttaac cctttcacat ccttatactc
    27121 ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa acaatgccca tctaattata
    27181 atttaattta attttaattt taaatcaaat gatatccttt ttagttaaaa aacaccaatt
    27241 atacaacatt tattattaaa attaaattat aaaattttat aacgttctca tttaacaaaa
    27301 cttaagttat taagacaaat gaataaacaa ctacgctttc ctcgaaacaa aaaaataaag
    27361 cacaaatgcg catttacgca ctcatgcttt attcgtacaa ttaattcaga taacggcagc
    27421 gcataggatg ccaataacac ttcctaaaat agctcctcct aaaatgtcag taggataatg
    27481 taaaagtaaa aaaatcctcg aataagcgat caaacaagca aataaaaagg ctaatattcc
    27541 tgcaactgga cttaattcga acaacgcagc tactgataaa aaggctagcg ctgtatgtcc
    27601 tgaaggaaaa gaagaagtct tcggtttact tgtgtaaagg acaagccgac gatttttttc
    27661 ataaggtctt ttccgtcttg tcgttttttt gataaaaagc aaaccaacaa aaaaatagat
    27721 caaactgccg ataagaatgg ctagaccata ttctctagta tcaggtatca agactaaaac
    27781 agcagcaagg agcaaccaaa taaaaccacc attagagata ttactgataa atttcaaaaa
    27841 attgattttt cgactatccg ttttccgttg cttggcgaac ttgttgataa tactatcatc
    27901 gattttagca atcaaacgca acatgttatt cctccttttt aagaaccttt tcttgcgtac
    27961 gatggataaa aagcaggact tgaaaataaa acaagaaact gaaacctaat aaagcaaaat
    28021 agattggtcc tgcaaaccag aatgcgacaa gaaggatcaa cgtgcctgcc aaatagaaaa
    28081 gcgcgctttt gaaatcatta aaaaacaaga aaaatgcagt aatccaagaa gcttcttttt
    28141 gttccagttg aagcaagatt ttgtaactaa agagaagcgc gcttaataga agcaaaaaca
    28201 acgtagtata catcaatgta tagccaattc ccccacccca atagcggaaa atctgataat
    28261 cgatcaatag aagaagaaaa taaacacttg ctaaactatc ttgctttcca taaagagaga
    28321 agttctcacg aaactttttc cagaatagtg aaccgagctt tttctcttca ttttttttgt
    28381 aatgataaat catatacaga actgttcgac ttgcagttcc taatgtgaaa acaggcaaga
    28441 cgcacaggaa ccatagtagc tgtaaatgga taagacgtgg aatccattca atgatttcta
    28501 agaaataatc attggctttt ttaccaatcg atcgattcat catccttttg aacctcctgt
    28561 caaatccatc ccttcaatga aataacgttg tgcaaaaaag aacagcaaaa tcgtcgggat
    28621 caagacaagt gttgctcctg ccatcagata attccattgt gtcgttgctt ggttctgaaa
    28681 aacttgcaag ccaatctgta acgtgtattt ttcttgatct tggatatata acagaggacc
    28741 taagaaatca ttccaagctg cgttgaagga attgatcgca atggtaatca aggcaggttt
    28801 cgtcaatgga agcatcaaat gactccaaat atacaaatga ttcgctccgt cgatttcagc
    28861 agcttcgatc aattcattat tgatcgataa gtaaaattga cgcatcagaa atatattgaa
    28921 cgcattgccg aagaacgatg gaacgatcag gggcaaataa gtacctaccc agccgatctt
    28981 ggagaataat acatattgag ggatcatcgt tacaaacccc gggatcatca tcgtagaaag
    29041 aaccaaagca aacatcagct ttttcccagg aaaatcaagc ttcgcaaatc cataagcaat
    29101 aaaagaattt gaaaggacat taccgatcac tactagcaca gtgatgaata atgtatttct
    29161 tgcatagcgc caaaaaggaa aggcggcaat cgtatccaga tagttttccc atacgatttt
    29221 ttctgggaaa aaggtcggag gataacgaac gatttcttcc attggtttca atgaagtaaa
    29281 caccatccaa agcaatggaa tgatcaaaat gattcctatt actgtcatga ggacataact
    29341 tagtattttt ttgtttgccg attcacactg gtctttttga aataatccat catttgccgt
    29401 cacctccaga ataatagacc cacttaggtg caagcttcaa attgacaaac gtcagaacca
    29461 acacgatcag aaacaggatc attgacatag ccatcgcata acccatatca aaagctacga
    29521 acgccttgtt ccacatatgc agattataaa aaagcaaaga atttgcaggt ccgcctgttc
    29581 catttttcgt catgacataa gcttcttgaa aaatttggaa agaaccgatc gtagaggtga
    29641 tcacatcgta aaaaataata ggagtgatta atggcaaagt aatacttttg aactttcccc
    29701 aaacgcctgc tccatcgatt tctgctgatt catataaaga tctaggaaca ttttgtaatg
    29761 tagctaagta aagtagcatt gctcctccag aactccatat tttcatcaaa atcaacgcag
    29821 gtttcgtcca ttgtggatca aacagccaag ctggtccagg gatatggaac cagcccagaa
    29881 atgtgttgat caatcctgca gatggagaaa gcagctgcat ccataagatg tagactgcta
    29941 ctcctgataa gactgccggt aaatagaaag ctgtacggaa tacactgatt cctttgattt
    30001 tttgattgag tactgtggct aaaaacacac tgaaaatcgt cgtcagcggt acattgaaga
    30061 tgacgaaata aacggtgttg tataacgatt tccaaaataa atcatcgtga acgaacattc
    30121 ttttgaaatt tgctagacca atgaaattca tcttggatgt caaattataa tcggtgaaac
    30181 taccaataac tgaaaaaagc ataggcccta gcataaatac caagaacccg ataataaaag
    30241 gcgagataaa taaataggcc catcttgctt cttttttctt tcggatagat aaacgcttca
    30301 aattttcttg cctcctctgt acctagacag tcaaaaatgg attattgatt ttgcttgact
    30361 aagttttcta ctgctttttg tgcatctgcc aatccttctt ttggtgattt tgttcctaaa
    30421 agaatctggt caatctgttc attcactaat gtcgtgtaat ccggagcatc taaaggaact
    30481 ggtgtgatca ccgtattttt aaagttttcg tccgccattt gatagatcat ttttccttta
    30541 tccgttaggt tgtcattatt cactaaattc tcattggctg tcttgctcgc catgatatca
    30601 aaactttttt cgccgaattt ttcttgtact tctggagaag aaagatattt gatgaattca
    30661 taagaggcct caggatcttt tgctccgtat ggtacttcca agacgaatcc gccaccccac
    30721 gaccaatggc ctgaaccttc ttctttttct gggagctgca tcacgccaaa atcaaaatca
    30781 tccggtgcat tttccatcaa actagagtaa taattgatat tttgggcacg catggctact
    30841 aatccggaaa taaatggatc agatacacca gacccaaatt cggcttctaa tttattgatc
    30901 gtatttttcc cataataatc ttgccagtct tggatccact ccaaggcttc cactttattt
    30961 ttagtatcaa tcttgacttt ttcatctttg tcaaaccaac tagtcccgtt atctgcgttt
    31021 agcgcccata catcagcccc taaattccat aatggataaa aaccgatgcg tgtaaattct
    31081 tttccttctt ttttatctag tttacgtgct gtttcttcta attcatccca agttgtcggt
    31141 agatcttgtt cactgattcc agcttcttta aatatctttt tattataaaa aatgacttgt
    31201 gtatctgtat tgaatgggat cgcataagga tcaccttcat attctactgt atcccaaagc
    31261 tgcggataaa aatccttaga agtgtctttt tctacatatt tggagagatt agttgcttgc
    31321 tgtgcttctg ctctttgggc aacggaattt atgtcctgta caataacatc gggtgcattt
    31381 ccagctgtta ttgcagataa agatttagtc cagatgtcac cccatggctg atacttataa
    31441 tttacatgga tctcgttttg tgattgattg aaatcctcaa tgatttcttc gatagcttct
    31501 tttcgtgcac cggatcccca aaaggaccag aaatcaatat tttttcttcc atcacttgtt
    31561 ttttcagaag aatttgctgc acttgaactg gcatcgcttg cattacttga tccgcatcct
    31621 cctaataaaa acaatgctga acttgccagc aaccctgtaa acaataattt tttcatattt
    31681 tacctccaaa ttttaatgta accgatgccg gttttaatat aacgatatca ttttgttctg
    31741 aaaataacaa tttataaggt atgattctaa aaaaattatt aatgatacca atactttata
    31801 taattcgcga gcgctttctt tgaatttttt aatctttcgt agcagaatga gaatgtatta
    31861 aaaatagatt aggaggaatt ttgatgttta gacaccagaa agaattgcag tttgaagcaa
    31921 aaccagatcg tccgaatcca ttaatcgcga aatatctgca ggagctgatc ggcggccaat
    31981 atggcgagat gagtgtagct atgcagtacc tgttccaagg ctggagctgc cgaggagaag
    32041 aaaaatataa agatatgctg atggacattg caacagaaga aatcggccac gtggaaatgt
    32101 tggcgacaat ggttgctcgt ttattagagg gtgcgcctgt tgaagatcaa gaagcagcga
    32161 tgcaaaaaga tccaacaatc ggtgccatta tctctggaat gaatccacag cacgcgatcg
    32221 tagctggctt aggacctcgt cctgctgaca gcgtgggcaa tccatggagc ggtgcctata
    32281 tcattgcgag tggcaacttg ttagcagact ttagagctaa tttaaatgcc gaatcacaag
    32341 gaagacttca agtagcacgt atttacgaaa tgatcgatga tcgtggcgta aaagatatgc
    32401 tgtctgttct ccttgcaaga gatagctatc atcaaaatct atgggaatgt gcagtgaaag
    32461 aattagaaga aaaagaaggt tcaatcttag tcccaagtac tttcccgcgt gacaaagaac
    32521 gaaaagacat tgcttatgac ttctataact tctcagaggg agaccaaagt agtgaaggtt
    32581 cttgggctag tggcaaagca ctggatggcg aaggcgaata tcaatggttg aaagaaccta
    32641 agatcgaagg cgctgcacca aaattagaac cagtaacacc aaaaatgtat gggacacctc
    32701 cattcaagaa ataaatatct acccgtagaa acacccgttt tttgttccac acgctttgtg
    32761 agcaaaactg actaaagtca ttaatcataa ccacccttca aaagggtggt tttctctaga
    32821 gctataagct caaatcaccg gccagcgcct aaagacgctg gctttcatat tttatgttca
    32881 agccatattg cctttgccag ttttgcccac ctcttaaaag gtttatgtac tactccttag
    32941 cccttaaaag gatcgtctta ctctcgtaca ctcagcttat ctaacgcact atcatgtttt
    33001 tcttgttctt gaatgtactt tttgatggtc gcttcatttt gtcctacttt tgtcacatag
    33061 tatccttcca cccaaaagtg tcgataccca aatttatatt ttagatttgc acaatctctt
    33121 gatgatctcc tgaatattgg ctcgatattg attataaatt atttttcttc tatactttgg
    33181 tgtgaataca atgtggtatt tacacatcta ctttgtacgg gctaaactat ttccctttat
    33241 tgctccaccc gcatagcaag tggttttgta tttcaatcgc tcggcgcgct caattaggtc
    33301 taaagacaat aaatctaaaa acccgctgct tcactgacag aaacaacggg ttttcagaaa
    33361 aaaatgaaat gaaaaagatc tcttacggca atctatttta tttttttgat tttttataaa
    33421 tggaaaatgc ttcaagaagt ccaagaaata aagccgtata aaaacaagaa cctatgaaat
    33481 ctttatttat tagatattca acagaaatcc cgataacact agccaacaat atatttatca
    33541 gaagtgtaaa ataaggatgc tttttataga atagaactag tgttgttttc atagactctc
    33601 ctttcttcat atttccaatt aatcacaaat tcacggcatt actagaaaaa ttaaaggggc
    33661 gcagaagtgg aaaaatactc ttcgactttc cctacaaacc aggccatctt gtgtttaata
    33721 actataccaa atctaaggta cctctaaatc taaattccat ttgtggcggt gacctccaat
    33781 ttttaactct tctatctccc atccagtata cataaccttg atacttcgac cacttatata
    33841 tcgaacacac aaatacctat ccattgcagg agtactacct aacttagttg ctacttgcat
    33901 ctaaataaca gctgaaaaga taggacttac tcttagtaaa gtatctgaac tattaatttt
    33961 taaactatca tttttatcct caactacatg cacatctgtt gcaacttcac tttctgtctc
    34021 atccagataa atgattaagg aatcgggaat tgcaatatct tcagctgacg ctggaatagt
    34081 tgagctaaat tagtcatcat atctttatta taaaataacc aacagcaaaa aatacaatga
    34141 aaaacaaact tggaactaat gtatcattaa taaatgttga aaaattcagc aactcattac
    34201 tgtatgattt tagcagccca accaacatat accctataaa agctaataag atggttagga
    34261 atcctttaga atttttactc attcatagac acttccttaa ataactttta ttatagaagg
    34321 tattgtaaat aataatattg acaaaaagtg actcaaaata aaaaaaacct tacattatac
    34381 tggaatgaac tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg
    34441 gtaaagtaat tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa
    34501 aggtactttt acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc
    34561 aattgcacat tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta
    34621 gcggtagatt tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt
    34681 tctcttttca ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta
    34741 tagaaattct ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata
    34801 gtttaaaatg acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag
    34861 ttgttctttt tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat
    34921 gtcgtgaaaa aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa
    34981 tcattcgttt aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac
    35041 cacactagta gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat
    35101 ataaaaatat tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa
    35161 ttttagccat gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat
    35221 caagagattt taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg
    35281 agtaacgtgt cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg
    35341 ttttacggtt aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt
    35401 ttcctacgga gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc
    35461 tttcttctga cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt
    35521 ctcataatac tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt
    35581 aattgttcca tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc
    35641 gttatcagaa ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag
    35701 cattatcatc actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc
    35761 ctttgtaaac tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga
    35821 aataaattgt agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa
    35881 cctatggatc caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc
    35941 agataaaacg ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta
    36001 taaacaaaaa gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa
    36061 actagcaggt gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac
    36121 attggttcaa cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc
    36181 tatttttgta gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca
    36241 acctcttatc tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt
    36301 tagattccaa gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga
    36361 ctcgcagcac gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct
    36421 atagatgcag tatatgcagc ttatgagcat ttcttctgtt ctctttgttt ttgggatctg
    36481 tgctgaagtc tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct
    36541 tctccattac taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg
    36601 ttacccaagg ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg
    36661 cgactgtgcg gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat
    36721 ggctacgata aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt
    36781 ctttttcttc attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa
    36841 ggtctttttt tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa
    36901 tccggtaaat ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa
    36961 cgtcacaaca agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg
    37021 ttgtttcgtc tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa
    37081 ataacgacca cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga
    37141 agttcgcacc aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac
    37201 aaactctcca gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa
    37261 caaacatgag aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct
    37321 tacgacctct gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg
    37381 caaaaatgta gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt
    37441 ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc
    37501 aaacgaaaaa tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag
    37561 ctttttcaca acctctccac taaacggtgt tcaatcagct aaccttcggt attagctcaa
    37621 aaaacggcaa aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc
    37681 gctgatttca caacctctaa tacacggtgt tccagaagta gcttcttcga aaaataagcc
    37741 gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg
    37801 accacttctg tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct
    37861 ttgatcaatt gaaaattctg cctaagtagt gatagatcct gtgtgacttt caacaataag
    37921 atacttccct ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggac
    37981 agtttttctt cagaaagtgc caggatattt tgtttcgtat acttgaccca ttgattgact
    38041 aattctagta gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta
    38101 cctacaggac agccatgata agtgatctgt gtctcatgat cagcgaaaat ccagtccaac
    38161 atttcttcta atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct
    38221 gtctgccata cgtccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa
    38281 aagtaataat aaaattgacc ttttcctgta tcagaagctg ctagaatatc tcggattgac
    38341 gtttcatgat agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt
    38401 gttttcaatg atattctcct ttttcatttc tcatagtcct atattataca gtaaattcat
    38461 cattttttct tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac
    38521 tataaaactg ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc
    38581 aattcctatc ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa
    38641 taaagccaaa gttcgtcaac gctgcatcca ttgcttcttg gccaggagca ccaattgcat
    38701 ctgtcgctac cacaacattg aatccactct ctaccaattc acgcatatgg gaatcgctac
    38761 acaagtttcc gttcattcca ccaagaatta ctgtgtcgat tccatttttt ctcaattgga
    38821 gcgccaaatc attgctttct ggtccaaaga ttttatgagg agagctgatg atcgtatttt
    38881 catccaaata aggtttcaat tcttcgataa aatcggctcc tgaaccttct tccactgatt
    38941 catattgact ttttctagca aacatctgct gctcaagcat catatgttct cctgcaccgc
    39001 caaattgcca tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc
    39061 ctttttcttt tgcttgtttt accatttcaa tcatattttc gatcgtattg tatttttcta
    39121 agatatcttt tgtcaaacca aagccaccgc ctgctggttt caagaactcg acttgcgggt
    39181 cagtgagaac tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt
    39241 attttaatac atcatttaga gcttcactca ttgtcggatg agaatagatt tgatctctca
    39301 ataacgtata aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag
    39361 aatcttccgc atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt
    39421 tgaatacacc tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact
    39481 tgaataattt gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg
    39541 ataaagtcgg agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt
    39601 tattctcgcc cttcaactga tccataatga ttcgataatc atctagagag atataagtga
    39661 actgcaatcc gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt
    39721 attcatccac agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa
    39781 gtgatgctgt acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta
    39841 cttcttggcc atcttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat
    39901 ctacagatac acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt
    39961 gcgcgatatc ttcatcttca cgtggaagaa attcagaatg ggcatcaaga acgacaactt
    40021 tagatccgta ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga
    40081 tcactaattt ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc
    40141 gacttgtatt caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa
    40201 tccgttcgcc ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag
    40261 aaagaaaacg tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat
    40321 aatttttggc gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt
    40381 cgattcctct ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac
    40441 atgttccgcc atacatacgt gttgactttt caacgactaa aaccgattca ccttttgaag
    40501 ctaacgcctt cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt
    40561 tttccatcat ttttccctcg cttctttttt acctaccgat tggtacaaaa atatgataac
    40621 cgataattgt ctattctcca aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag
    40681 cggtcctttc acgtttttag tctggttcga tttcctttat tcttctaaac aatcgttact
    40741 tctacatcga tatttcctct agttgctttg gagtaagggc agaattcatg cgcatcttct
    40801 gcaatttttt gggcttcttc ttcgctgatg ccatcgatat gcacttctaa tactacaccg
    40861 atctgatagc cgccatcatc taaattataa agagagacac gtgcttttac tgtggactct
    40921 gctgtcattc ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca agaagcataa
    40981 cctgctgcaa ataattgttc tggatttgtt ccttctgcat ggattcctgg aggagtgatt
    41041 ttcatatgca tcgagccatt tggtgcctcg acttctccat tgcgcccgcc atggttgatc
    41101 attgctgttt cgtatgcttt tttcattgta ctgctccttt cgattgacca tttgctactt
    41161 ggctataatt atagtgtatg acaagtaagg cgctttctac aaaaattctg cttattcgaa
    41221 taccagaatg ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa
    41281 cagcgtcata tttgttccaa aataaatagc aatcttttct acataatctt ttatatgatt
    41341 gattttaaga tacatatata ccagaaagtt gaccccataa accaatactg ttaaaaaaat
    41401 aaccatattc gttaccattt tttcctcctt ttgctggtct ccctaccagc actcgattta
    41461 accataccac tttttttgcc gtccaagcaa ctagcctttc aaaaagactg gtttattctg
    41521 ctgttaatga ttcaagatca gaaaaatgtc cgaataattt tgctaatttt actaacgcca
    41581 acttgttctt tccgaattca tcattttcta tctgtaatac tagtaacggc tcatgtaagc
    41641 ttaaacggat caaaaaccag ccgtttccgt atggctcttc tagatcatat cgaatacctt
    41701 cttcattttc cgggtcgaag ttcatccctt tttcttcttc caacagtcgc ttcatttctt
    41761 ctaatatctt gtttccttgt tcgcgtggtt catcgctgaa caattttaat cggatttctt
    41821 gtacttctac gggctgtttc aactctttga tttgttcatc taatgtgata ccttttcttt
    41881 ttaattctgg caaaagcatc aggatctttg cgatgacata tgcaccatca tccagaaaat
    41941 agttttcctt gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta tctttcccaa
    42001 gacggttaag ttctaatgct ttattgatta catttcgata gccggaaata taacgaactt
    42061 ggatgcctcc tttttcttca atgaatcttt gtagatgaga agaagtaggg gaattcgtca
    42121 cgattcttgt tcctggatgc tcttttaata cgattgtact taatactgcg atcaaattat
    42181 ttcgattgat cactgttcca gaagcactga caactgcaga acgatccaca tctgtatcga
    42241 aaatgatccc aagatctgct tcatttgcta aaacagcctg cttgatactt tccatcgctt
    42301 ctttattatc aggatttggg atatgattag gaaatcggcc atctggttcc aaaaattggg
    42361 aacctgttga atccgcacct aagctattca atactttttc agcaaagaat ccaccagcac
    42421 catttccggc atcaacaatg attttccaac catttagtgg tttgctttct agtgttccaa
    42481 tccccttttt aatttttgtg actaaatctt gcgcataagg tgtcaaaagg tctgcttttt
    42541 ctacgcttcc agtttgttcg ctttttattt tttgctgctg tgataggata aaagcaatat
    42601 cttctttttc tgctcctcct tctgaggtaa agaattttat tccattataa taatagggaa
    42661 gatggctagc agtcagcata accgcagcat cgcacgagaa ctgttcaaat tgagtggaca
    42721 taaacatagc aggcgtagta gctaattcga aatcgatgac atgtatccct tgttctttca
    42781 gactttggat caacgcctct ttcagtgctg gtccgcttat cctactgtca tgaccaatcc
    42841 caatcgtcca ttttttattt ggatcggttg atttcttctt tttctttatc cagtttgcta
    42901 gtccccagcc gatctttttt accatgtctg tcgtcaagtt tacttcatat ccttcaactg
    42961 gtaatgcgat tcctcgaata tccgatccat tttgcagcgc tttcatatct ttcatataaa
    43021 agtcctcctc atttattccc tcattttttc cagcttttct gcaaatcgct gtgcttcttc
    43081 ttctgagcgg caaataataa tgaatgtatc tacaccggcc atcgttccga caacttcagg
    43141 gtaagccact tcatctagcc aattcgttac cacatcggca ccatttcgtt cagtcaatac
    43201 gataaccatg aactgaacag actggatacg caaaacctcg cgtctaattg ctgtacgcaa
    43261 acgttcttca tttaaagaaa cagatggctg gttaaataat gtatagcgta ctattttgtt
    43321 ttcattttga ctttttacta acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt
    43381 cacatcggcg cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc caataggttg
    43441 ttctgtaatg atctggcgga tcagtgcttg tcgctgtttc tttttcatat tagacacctc
    43501 tcaatgttga ttatagcaaa agataagtaa tatttcttca tattttggta ttttttactt
    43561 ttattttttg ttttcattca ggttgctaac ctttttctat tttaacacgc aaaatcgtct
    43621 tcattttttc aggtgctgat tttcttggat cgctgatgta aatctctttg tgttctttag
    43681 aagctctttc atagccttct tttgcgatga atgcttctaa tttctcaaac gttgctggtt
    43741 cttcggcata gggcccgacg tgtaagattt gggcacaaag accttcttcc atctttttga
    43801 atactacttg ttctttcaag tcttccggaa gtttgctaga acgctgaatg gcttcttgtg
    43861 ctatcgcttc tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt
    43921 ttttcatcac tggttcatgc aaatactttt cttgcagtcc ccattgtcct tccagtggat
    43981 agacagtata catctgataa tcaggaatca gccaattttt tttaggcgac atgcgtattg
    44041 catagctgac tgcatataga caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc
    44101 cgatccctct gatgctaaag aatgattttt taggaacctc tagtatttgc gggctttttt
    44161 tcatactata gctcttgtct ttacgccatt caattttttc catttttctt cctccgcgaa
    44221 attccattta gttatttatt atcatacctc tttttgagtg acttggctaa tcgtgttgac
    44281 actatttttc agtttgtgct agaataagtg aagtaaataa atcgtacaga taacacgttt
    44341 cggcgtgtcg agagagaggc ggtttctatt tttataatag aaatcactct ctttttttac
    44401 gaaatttttt agaggtgatg taaatgacaa aacaaaaaca gctgctttta gtacctgcag
    44461 taattgcaac agggatgatg tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa
    44521 ctttcccaac tcttatcaga cagtttgatt taacgactgc acaagtccaa tgggtaacaa
    44581 cgatttatct tttgatgatt tcgatcattg ttccgctgtc tacttatttg aatcgcaatt
    44641 ttagtctcaa aaaactcttt ttagcttcaa atctgctttt cttggcaggt ataatgatcg
    44701 atttcttctc tcctaccttc atggtcctat tgtttggtcg attgctccaa ggtgcagcga
    44761 caggtatcgc tcttccgttg atgttccata ttatcttgac atttgccccc ctcaatcgac
    44821 gaggcgcgat gatgggtgtc ggtactttga caacggccat tgctccagca attggcccca
    44881 cttatggagg cattatgact tcacatttta catggaatca tatcttcctg tttcttgtgc
    44941 ccgttttgtt tctttctttg attatcggct ggtttgctat tcccgagatg gcagtacaaa
    45001 aaagtggtta tttggattgg ataagcgttt taggaatttg tcttttgttt agtggaagtc
    45061 taacattttt gagtacgttg ggaacagttt ccagctggat tgctttaatc atcggcggta
    45121 ttggctggtt ccttttttac tatcgaagta aaaaagtgga gcatccactg atccgactgt
    45181 ctattctttc aaataaaaca ttccgcttgt ttttgattgg cttcttagtt tatcagtttt
    45241 tattacttgg tgtatctttt gtcttgccga atttccttca gatcgttcaa ggaagtagtc
    45301 cttttgtcgc tggattagcg atgcttcccg gcgcagcggt tggtgctttg ctttctcctt
    45361 tttccgggaa aatgctggat caatatggtc cgaagaagcc aatttttgct gggctgattc
    45421 tttctttgat tggttggcta gcattattcc tactcatagg acacgcttca gtgggtgtat
    45481 tgattgcttg tcatgtgttt tatatgatcg gtatcggatt atcttacagc aatatgatga
    45541 ccaccggaat gaatgcactg aatgaagaac tacaaggaga cgggaacgca atgttcaata
    45601 ccttgcagca gttttctggc gctgtggcta cttcattagt agcggtcatt atcaattacg
    45661 tccagcatca tacttcccat aactatgaag tgtctactac attaggatca aaagcagcat
    45721 taggcgtctt gttgctgttg cttctcgtaa gctttgctcg attcgcctat tatctgttct
    45781 ttgctaaaaa agcgtagatt ccatatacgg caggtgttgc atctgccgtt tttttgtgtg
    45841 aaaatggttg tttgtaaaag gaggcttttt tatggaaaaa attgccatta tcggtggcgg
    45901 gatcattggt atgaccctag ccagttactt agatacatca aaattcaatg ttgctttatt
    45961 tgatgaaggg acaggacagg caactaaagc tagcgctggg attatctccc cttggttgtc
    46021 gaaacgcaga aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa
    46081 acttataaaa gatttccaac ttgatcaaac gatttacgca caatcaggga cacttttgct
    46141 tcgacctgaa aaagatttag ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc
    46201 gccagaaatt ggaaacatcg aatggctttc acctgaacag acatctcaag cacttccgtt
    46261 attgaaacca gcagtttcac tgcgtatcag tggtggtggt cgtctggatg gaaaagcata
    46321 tttagaacat cttggtcagc gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt
    46381 cactttgcat aagaagagcg attcttggct catcaaggga ccgtttggag aatacacagc
    46441 tgatcgtgta attttgacac ctggcccggc acttaagcct ttattacaag aattaggcta
    46501 tgctgcagat atacgtccgc aaaaagggca gctcttagca tttcagacat cttataagat
    46561 gagtcaagac tggcctgttg ctatgcttga cggagaagct gatttcattc ctttcatgga
    46621 tggaaagatc cttttaggtg ctacccatga aaacgatcaa ggctgggatt tggctcctac
    46681 tagcgaagct tataagggat tgactaagag tacagaacag ttcttgactg atccagatca
    46741 gataaaaaac tggccatacg agttccgtgt aggtacccgt gcttatacgt cagatttttc
    46801 cccatttttc ggcgtactgc cagatgatcc aagcttactg gctgctagcg gtctaggctc
    46861 ttctggcttg accacaggtc cttatatcgg ctatctatta gccaacttct taaatactgg
    46921 tcattggcct acggaagatt atcaaaagga catctccacc tatattacga aaaatccatc
    46981 atagaaaaac agctttttca agtaaggatc ccgagcgata ttgcgcatgg attacttctt
    47041 gaaaaagctg ttattttagt tcaatttctg aatcaaattc tccaaacttt cttttgcttc
    47101 atctggcaag agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg
    47161 ttgtttcacc agatttttat aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg
    47221 taaatagtgg atgtcttcaa atggaccaaa cgacttgaat tctgccgttt gttgcacgat
    47281 tctttcaacg attgacaatg tcgtttcagg tttgattttt ttatcatcga aatatccagt
    47341 attcaggaca tagcaggctg tttgctgttt caaaaacaac tctcgaaaag cttgatagtc
    47401 ttccctaaga ggatagacac ggaaaggatt agcaaatggt tcaatgacta gctcatctag
    47461 ctgtcttttt gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa
    47521 agacgctgct aaaactgggt cattgatctt gataattggc ggaagcgaat catctttcat
    47581 gatccaaaaa acagcatcga tcttttcgct aagatgatcg actcggtcgg gtgtcgcata
    47641 acgtgatttg actgtccgcc catttccgtt tcgtatatct cctgttacta ggactttttc
    47701 tcctttactt gtacatgtca cgcccacatt ctgacaagta aggaaagaag aaatggccgg
    47761 atcatcagac ggatagtctt gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa
    47821 accatttttt cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc
    47881 cgtatgtttt gctaaggtaa tcgttgattt tccagaaccg gacaacccaa atacagccat
    47941 cgtataggtc tggtcattac tagcatattg tttcatccca ccgtggcagg caataaagcc
    48001 attgcgattt cccaatgccc aagcaagcgt caaagtcgct ttttttaatt ctccaaagta
    48061 acgtaatcct agcacagccg ctgtattatg gagagcatca aacaaaatga gtccatttgg
    48121 aaatgtttca tgtgtccatt cgggatcaca aaagatataa agatctccct catcaaagga
    48181 aacagacttt tgataagtct ctaagatctt cgaattcgct ggctgaaaat tcagcaaata
    48241 agaatacaaa ttgttctcaa atccttctgg aatcagcaaa tgactttgca cagaaaattc
    48301 ttcagatagg ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac tttgatacac
    48361 agcttctctt aaaattttct cgtatttgct ttgttccttg tctaagtcct caaagatccg
    48421 ccgagcactt gctgttcttc ccacaatgct tccatcattg aatactagtg tcctaacatc
    48481 agctggtaaa tctagttctt cagcatgtga gattgttaaa tctgtgatga tcgttcctgg
    48541 actccctttt gctagttgat aagctgtctt cctatcaaaa acttcatgaa cattattacc
    48601 gtaaaatgtc gtttctacaa tcgatcgaat actggataac agtggatttc cacttgtgat
    48661 ttcgtttaat tcgagactct cttttgtact catggctcct cctcattccc taaacaaaaa
    48721 aacgttttct ttattataca ttattcgagt ttttatttgt actgctgcgc tgtgggaagc
    48781 aaacgagtta tttttcacac aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc
    48841 atcattttcc ttttatcggc ttagtaccag ccgtttgcta accagaatgt tttagcagca
    48901 tcccatgaac cataacgaga tgttacatat tgttctgcta cacgttcttg attggcagct
    48961 gaatagtcgc cgtttaagta tgaagcatct aattgataac ggccgatgta acgtccgtta
    49021 gtagctgtgt atgaaccgcc tgattctttt tgtgcgatcc attcttttgc agagcttgaa
    49081 gttggcgctg ttgcagcagc tggggcagca ggagctactt caactgtttc aacaggtgca
    49141 acgtatgtat gttcttgcgt tgctgtttca gttgtagatg ctgtattttc tgttgttttg
    49201 ttttctgttg ttgctgaaga atcatttgaa gttgggattg ttaattgttc accaacataa
    49261 atgcggttga tgtttgcgat cgaatttttt tcagcaattg catcgatcat tgagttgtct
    49321 cctgcaaatt tttgtgagat cttagataat gaatcacctg attttactgt ataaacttca
    49381 tcagcgtgag catttgtagt tcctacaaac attgcagtag caccagcagc aaaagttgta
    49441 cctaaaacaa ttttcttaaa tgaattcata ttaaactcct ttttttctcc gttttttatt
    49501 ctttcgacgc tattaacttt atcatttaga aatatttcat aggtaaaaga aacatgacat
    49561 ttaatgacaa aataacgaat tatttaatat ttgtaacatt taaacaaata tttttttgat
    49621 acaaaaccat aaaagccttg ttgtataagc aaagtaatcg tttttctatg tttttttatt
    49681 tttgaatgaa aaacgcagat tctgtaacat tcactgcaat aatccataaa aaaaagcaaa
    49741 aaaatttttg atttttatca aattctctta aaaaaatgta aaaaatacaa aaaaaccagt
    49801 aattgttaca acaattactg gacaaaaact ttgaataatg ttaactattt ttttatttta
    49861 actttctccg caataatttc tgggcatgtt ccaattcgtt tataacgact tcttctgttt
    49921 ctttagctaa tatttcatct aatacatcaa gccatttttc cggttctttt gttcctagac
    49981 gtccaatagc ccaagctgct gttcctctga tgacagggcg gacatctgtt aaacattctg
    50041 aaatctcagg caatgccgtt cgatcaccta gattagctaa agcaatcaat gcgtttctct
    50101 gcaaaggttt cttcccacgc catgaaccag ctaaatgccc aaattgctgt ttgaagtctt
    50161 tattagacat cgaaagcatt ggctttaatt ttggatatat ttcgtctact tttggttcca
    50221 tctcttcatg aaaatgaaaa tcttttcctt gattgtaagg gcagactaac tggcagatat
    50281 cacatccata aatgacattt cgcatttttt tacgatattc ttccggcatc atcccttttg
    50341 tctgagtttg ataagacaaa catcgctggg cattcattct tccgtcacca agcaatgctt
    50401 ttgtcggaca agcagtcacg cagcgcatac agtcaccgca accaaattct cctggtttat
    50461 caggctcaaa tacaatatta gtaacgatct caccaagata gacaaatgaa ccgaactctt
    50521 cagtgatcaa caatccgttt cgtccaataa atcctaaacc agctcgctgt gctacagcga
    50581 catcgatcag ttctccagta tctacttgcg gagctagacg ccattcctct tctttttgcc
    50641 atttttctgc tcttgtttgg atgaattcga tcaatcggtt caaacggtct ctgagtatat
    50701 catggtaatc gatcccccaa gaagcacgag caaactgccc gcgtttttca tccctaggca
    50761 cttcttcatg gatcctagta ggatacgcaa gtgcaatgga aatgatcgat ttcggctgat
    50821 caaatgtcat ttctggataa agacgctcat cgatatttgg atgttcaaag ccagaagtat
    50881 ggccagctgc tttttgttct tccaacgatt cttttaatga atcaaaaggt tccgccgtgg
    50941 tgaacccgat tttgtcaatt cctaatcgtt tgctttcttg tatgatttct tcttttaatg
    51001 tcatggtctt cctcgcttgc tgttaacggc tgtataaccc ataatatagt ttttcttttt
    51061 tcagctggac aaactggatc tgctgatcgg caatcttctt ttcttccatc catttttttc
    51121 ttgcagcaaa tacttcccaa taatttgttg aaattaattt tcttttgtta tctaatgcat
    51181 taaaaaaatt caatgaaact ggttttgtac ttaacagttc atggagcctt acctcttccc
    51241 atggcgaaaa ctcagacaga tcgcgggaat acaatacttc ttcatagaga tttttttgct
    51301 gtgtgtacgt gaatgtttgc gggattttcc caggcctagc aatttctact agacgattat
    51361 aaaacatatt cattaatttg ctgttggcaa taaattgact tttataatag gccgactgga
    51421 tctttttgat tactccttct gctttgatga ctgaaaataa tgcaggccat gctttgacgg
    51481 ttatttcgtc gttttgttta actaatacgc caggtgtgaa gccgactgga gaaaggaaca
    51541 caaaataacc gataccggta ttttcatcta aaggctgaaa ttcatcaaac aaccatttgc
    51601 ctatgtacag ctcttcatct ggttctgttg tctcattttg atttgctatg aataatttag
    51661 ctaattctgc ttcaagaaat tgaaggtctt gatccttgtc ggtattttcc tgctggctga
    51721 tcaatcgaaa gacttcttct ttaatcgtat atttatcttt acgtgtaaaa ttcatagcca
    51781 caccttcctt ccttactcta actataacgg aatttcttca aaaaaggtat ctgtacgacg
    51841 aaaaacagca taattttttt tcatatctct tataaaaaat gcaaggtctg ctcctcaagc
    51901 tagaccttgc acttttcagc tttcttttgt ttgtgacaaa ccttcatgga tcgtttcaat
    51961 attccatttc atcatctgat agtaagaatc tccttgttct ccttcttttg ctatggaatc
    52021 agtgaacagt ttatcataga ttttcaaacc cgtttctttt gctacccgtt ccatgcttct
    52081 tgagtctaca ctggtttcca cgaataaaac cggtacttct tttgctctta tctgatcaat
    52141 gatcgctttc atttgatcag gcgtaccttg actttctgta ttgatctccc atatataagc
    52201 tgctggcaaa tcatatgctc gtgaaaaata cttgaatgcc ccttcacttg ttactaataa
    52261 ttttttgttc tcttcgatag aagcaaaact ttccttcgct tgtgtatcta attcttttag
    52321 tttttccaca tatgcttctg catttttttt atagagtgtc gcattttctg catctttttc
    52381 agagaatata cgtgcgattt cctccacata ttttattccg ttagataggt ctagccaagc
    52441 atggggatct gcttttgtat gttcttcacc gctagttaaa taaagaggtt ctacattttt
    52501 gctaactgca aaataatctt ttccttcttc ttttttagcc gtttccatca agttatcgaa
    52561 ccagctgtta cctgtttcaa gattcaaacc gttgtataaa ataacatctg catcgcttgc
    52621 ctttttgatg tcttctggta gtacttcata ttcatgcgga tctgttccga ctggtacgat
    52681 actgtggata tctatttgat ctgttccgac ctcttttgcc atgtccgcca agatcgaatt
    52741 ggtagccacg actgacaact tttctttact tccttcatcc gcttggtcag aagcctgctt
    52801 tccgcaaccg gctaatacca agaaactaac tgccaagatt aaaaagagtg atttttttgt
    52861 tttcatctat ctgcttcctt tctgaaataa aattcctttt gttggagaaa ataaaaaagc
    52921 aaggataaaa aatgcggtac atgctaacac gatcgtagct cctgatggcc aattgtaact
    52981 ataactgaaa aacacaccga caactgcact tagcataccg attcctgcag ctgtaatgat
    53041 cattttcagt aaatggttcg ttagcaagta agccgtggcg gctggtgtga tcaacatggc
    53101 aatcaccaag attgttccta ctgtctgtaa actgactaca gccactaatg tcaaaaagaa
    53161 catcaaaaga taatggatca gcgatgtgtt cagcccataa gcttttgcca ttgtcggatc
    53221 gaatgatgtg atcttcaatt ctttataaaa gaaaaagaca aatatcaaga cgattccact
    53281 aacgagtgct gtcagcaaca aatcgctttc cctaacagct aagacattcc caaataaaat
    53341 atgatacaaa tctgtcgcac tctgggcata tgaaatcaag ataatcccta atgcaaaaaa
    53401 ggagctaaag acaattccaa tcgctgtatc atttttcaat cggctccgct gagtcacgaa
    53461 accaatcact gctgctgtta gcatcccaaa agcagtggct ccaaagatgt aactaaaacc
    53521 aaacatataa gaaatggcta cacctggaag aactgcgtga gagatagcgt ctcccatcaa
    53581 agacatccct cgtaaaataa taaaagaacc gatgactcca gaagcaagtc ctacaagcat
    53641 ggaagtgacc aaagcgtatt gcaggaattg ataattcatt aatccatcaa taaaggattg
    53701 gatcattttt tctctcctcc ttttactaaa tgtctgatga tctcaccata ggccaattga
    53761 aggttcttcg atgtaaagac ttcttgtact ggaccggaag cgatcagctg tttatttaaa
    53821 agaataacct catcgaaata ttcttctact ttatgaagat catgatggac gatcaaaatc
    53881 gtttttcctg aattcttcaa ttcctgcaag atgtcaaaga tctttcgttc acttaacgca
    53941 tcaatcccta cgaatggttc atctaaaaag atccattctg ctccttgggc tagagctctt
    54001 gcaataaaaa ctctctgaag ctgtccaccc gatagttcgc tgatctgtct tttggcatat
    54061 tcttccatcc ctactttttt caaagcttgt cttgcacgtt ctttttcttt tttcccaggt
    54121 ctttgtccaa ttcgtaaaga tggatatgtt cctaaaagta caacgcctaa tacatcaatt
    54181 ggaaaggaaa gatccaattc acttcgctgt tccacgtagg ctattttttt acggactgag
    54241 tttaccgact gtccagaaaa ggtgacttta ccagtagtag ctggtatcaa ctctagcaaa
    54301 gatttcataa atgttgattt tcctgcaccg tttggtccga taataccggt aattttttgt
    54361 ggatttatcg tcaagttgat agttttcaaa acagttttcc cttgataagc ggctgaaagg
    54421 tcttgaatta caatggccgt acgttccaaa tcttacactc ctttttaggt ttacctaact
    54481 ttttatttaa atatagttta actaaaatgg aaagcatttt caagtagaat gatgaaaaag
    54541 tttttttcga attttcctcg cattttatga aaattttctg tagaatcaca tcaagagttc
    54601 aataaggcta acctaataat gaaaaacatg tgttgaaacg tttgattatt ttttcttgtt
    54661 gactcaacaa cgaaaggagt ttttgtatgg aagaagaaaa gttaactcga agtcttagtg
    54721 cccgtcatat ccaaatgatc gcactaggtg gaacgatcgg tgtcggtctg tttatgggtg
    54781 cctcctcaac catacgttgg acagggccct cagtgatggg agcctatgcg attgcaggat
    54841 tctttttgta tttgattatg cgtgctttag gcgaaatgct ttacgttgat ccttcgactg
    54901 gttcttttgc aaattacgcg agcgagtaca tccatcctgt ggctggctac ttgaccgctt
    54961 ggagtaatat ttttcaatat attgtcgtag gaataagtga agtgattgct gtaggatctt
    55021 atatgaatta ttggtggcct gatttgccgg caattattcc cggaatcatt gtcgtcttgt
    55081 ttcttatgct ggcaaatctt atttctgtca aggcgtttgg cgaattagaa ttttggtttt
    55141 cgatcatcaa agtcataacg atcattttga tgatcattgc aggtcttggg gtaatcttat
    55201 ttggatttgg taatcatggt caggcagtag ggatttcaaa tctttggaaa aatggtggat
    55261 tcttcactgg aggcgtcaaa ggatttttct ttgcattatc gatcgttgtt gcttcctatc
    55321 aaggaatcga actgatcgga atgactgctg gtgaagcaga gaatcctaaa aagacgatca
    55381 ttgaagctgt ccagtccact atcggtcgaa tcttgatttt ttatattggt gctattttta
    55441 tcattgtgag tatctatccg tggaatcaat tgagtgagat tggttcacct tttgtacaaa
    55501 ctttttctaa agtcggcatt acatttgctg caggactgat taattttgtc gtgattacag
    55561 cggcattatc aggttgcaat tcagggattt tcagcgcaag tcgtatggtc tatacacttg
    55621 ccataaatgg aaagatgtct aaaaaatttc tgaaactatc tcgtcacggt gcgccttttt
    55681 atcctgtcgt agcaatctca ggtggaatct taattggtct gatcttaaat tatcttctac
    55741 caatgttcat caaccagtca gaaaatattt tcgttttcgt atacagctca agtattttgc
    55801 ctggtatggt tccttgggta gtgattttga tcagcgagat tcgcttcaaa aagaagcacg
    55861 cagataaaat gcatgatcat ccttttaaaa tgccactatc tccttatacg aattatgtga
    55921 cacttgcttt tttagctatc gtattgattt tcatgtttat caatcctgaa acgagaatct
    55981 cattgattat tggactcgtt ttcttgcttt acatgacgat ccattacttt gtacgcgaaa
    56041 aaagaaccgg aaaataaaaa aagaacgaag cttcacctta gtggaagctt cgttcttttt
    56101 tattgctttt atcaattcaa ttttagcgtc tcaccgggag tcaggaaata attctccatg
    56161 ctcatcccat tcagcgacaa gatcgtttct actggtacgc cagttcgttc ggctatttga
    56221 tttggacctt ctccttccaa aactacggtt gtcgcagcct gtgaagtcgc gctgttggta
    56281 ttagtttcag aggaatctgt tgtgtcttgt gttgttcgag cttgcatttc agaagcttct
    56341 accgactctg ctgtctcaga atcagaagat gttgtgtctt gtgtgcttgt tgactctgct
    56401 tgaagagagg cgctagtctg caaataaata tcggaaatct tagcgacatg attcgtataa
    56461 ttagccgata attgattgat caaagtatct aagtctacac cgtctttatt ttctgctgcc
    56521 tgatacgctg cacgtaattt tctgatccct tcgtcggaga ttgcttgaag ctcacgtgct
    56581 ttttggttag caactgctga taaaccagct attccctctt gatttccctg aatttctgtc
    56641 tggtattctt caatcaatgc aggcgtcttt tcttcgatct ttttcgaata atcatttacg
    56701 attgcttgaa gttgctctgg caaaccattt gctgtttttt cttcagaagt ttgtgtctct
    56761 tttgttttag ttgatgactc acttgtttgt ttcgtctctg ttttctttgt cgttgtttcc
    56821 gttatggtcg tacttttcgc ttctgacgaa ttttttccat gaaaatatag acccgcaact
    56881 cctcctgcta tgatcaaaaa cacggcaact gctgccccaa tcatgatcat ccgcttacga
    56941 gctgcctgtt tttgctgtct tcttcgggtc atccgaacat tttctcccca ctcattttca
    57001 tcatacattt ttttcaccac caatttttat gtataatagc gttatcaaac gcttcatatt
    57061 cttattgtag caaataattg tcgctgtgca catttttatc tttgttattt ataagaaaat
    57121 ttcaccagtt gagatgattc caagattgtc cgtcgatttc tccttgcagc tgcattgttc
    57181 gttcgtctat ctcagccatc agtttcttta tttcatataa gagcgcacgg tcttgatatt
    57241 ctgatttttc gcttaattca tctaattgtt ggatcagcca atcatttgtc ataatgaagt
    57301 tcctttcatt tcttgcagtg taaaggtcaa gtcttcgatc tgctcttttg cctcagtcag
    57361 tgcttctacg atatatgctt gtttttcaga ttcttcttcc tgcattttct gattttcctg
    57421 tactctttga ataaaccagt caatccgctg agtcagcgat ggtttgcctt ccaatgagag
    57481 atactgctga taagcaatta gcttctccca acgtttttcc tgggataaat acataaattg
    57541 tgctaaaacg aatggcggaa gataggtcat atggaattta tttgcgattc gctgatacgg
    57601 cctcaaaaat tcggaaatcg tatattcttc ttttcctcca gcttggtatt ctttttccgc
    57661 cacacctgtt gtcaatacga ttcctagttc tttccctttt aatacatgtg ctgctcgttc
    57721 caagacatta tcttgccaaa tttttaaatg agcaggagaa ctataccagt aaaacggaaa
    57781 ctggaaaatg atccgatcat gctgggccaa gagctgtagt tcagtctcta tatctatttt
    57841 tccgtctgga taacttgatt ccaaatgatg ccaagtagct ggtaaaccag cagaagcttc
    57901 tttgaaaaat tgctgattta aagattcttc gatcgttgga tggctaatga tgataagtgt
    57961 acgcatccct tttcctcctt tttagtcttc gctttacagt gtagcataac cgccacaagt
    58021 ttcttttgcc gatgcttgtc attttaacca gactaatcag gttgtccaag aaactgcggt
    58081 ttgtttttcc aactgttttt tcaaggtatc caaagcaata ttcttaaaat tttcgttttt
    58141 catacgttta cgatagttcg tagccgtttc tccggtactc tttttgaaca ttcggctaaa
    58201 tacgactaga ctattgaacc ctacttgata ggcaatactt tccacggatt catccgtcag
    58261 agaaagaaga aacgcccctc gattgatccg taccccgatc aaatattcct tgatactggt
    58321 acccatatga tttttgaata ctcgcgacaa atggctccga ttgaccgcta aatgctgggc
    58381 gatttcctca acagacaatt tttcagcata atgtgcctcg atgtatttga ctgtttcaac
    58441 agctaaagag gaatgaacct tttgtacacc accgctgacc tttccgccat cttttaaaag
    58501 gactgataaa aaacggtagg ttaatgctgt taactgtaat tcattttcca aggtttcttc
    58561 cgtatctttc atgcattcta aaataatgtc gatatatttt tgtatgtcag aagagaccat
    58621 cgtgtattgg tcttctctga aaagcgactg ccgaatgatt tcttctgcag cactcccacc
    58681 aaatgaaagc cagcaataca tccacggatc ttctccatct gataagtaaa acgtcgagtc
    58741 tcctggacga atcaagaaca gatcaccttt tttcaattgg aattgttgat ttttcacatg
    58801 ataagatcct ttgccttcca tcacgacatg caggatgtaa tcattgcgaa tagcaggacc
    58861 aaaagaatga aacggcatcg ttttagagaa accacaaaaa tcaaaataca attctttttc
    58921 tacttgttga tgttcctttt ttaaaaaaat cgattgctcc acgtctatcc ctccttactc
    58981 ctttagtata atgcaacaaa aagttaaata aaagtcacag aaatatatta aaaaggaagc
    59041 gatttcttgt taagtttaag tcattaaaag atctttgtta ttcgttgtaa ccggtttact
    59101 tagtattata tattgaacag gagaggaata cagattatga aaagcaaaat gatccaacgt
    59161 gtgtcctacg ctttaggcgc ttttggacac gacacattct atgttacatt aagcacttat
    59221 tttatggtat tcgtgacaag ccagatgttc gcaggaacag ataaggcaac gaatgcaaaa
    59281 atgattgcta cagttacaag cttagttgta ggtatccgct tagttgaaat tatttttgat
    59341 cctttgattg gtggcatcat cgataatact cgtacaaaat acggtaaatt caaaccatgg
    59401 ttagttatcg gcggcgttgt tagttccgta ctcttggtag caatctttac caatttcttt
    59461 ggcttagcta ctcataatac gactttattt atcatattat ttgcaatcgt tttcatctta
    59521 ttggatagtt tttattcatt taaggatatc gctttttggt caatgattcc agctttgagt
    59581 acggaaacaa aggaacgtga aaaatttgct acgtttgctc gtttcggctc ttctctaggt
    59641 gcaaatggta ctactctagt cgttgtccct atcgttactt tctttaccta tcttgttact
    59701 cataaacata ttgaaggcgc ttcaggctgg ttctggttcg gtgtgattgc tgctgttatt
    59761 tcaggcggta ctgcgattat cactgccttg ggcacaaaag aagtcgatac tgcgatccgt
    59821 cagcaaaatg aaaaagctgg gatcaaagat gtgttcaaag cagttgctca aaatgatcag
    59881 ttgatgtggc ttgcccttgc ttatatcgct tatgcgattg ccaatgtagc aacaactgcc
    59941 gtattattct acttcttcaa atatgtactt ggccaaccaa ctgaattttg gatcgttggt
    60001 ctagttgctg ccttacttgg tttagtagct gttccactat tcccaatgct tacacgcgtc
    60061 atctctcgtc gctacgttta ccttggaggg atcgcaatga tgatttcttc ttactttttc
    60121 tttacgatcg caggttcaag tttgccaatc gttatcatcg ggctagtatt gttctacttc
    60181 cctcaacaat tgattttctt atccgctttg atgacgatca cagactctgt tgaatatggt
    60241 caatggaaaa atggtatccg taacgaagct gttactctat caattcgccc tttactagat
    60301 aaaatcgctg gtgctttgtc taacgggatc gtagggtttg tcgctatcgc ttctgggatg
    60361 actggtaatg ctactgctgc tgatatcact gctcatggcg taacgacttt caaagcttat
    60421 gcattctatg ctcctgctgc tttgatgatt ttctccgcta ttatttttgc ttggaagatt
    60481 acattgacag agaaaaaaca tgcacaaatc gttgagaaat tggaaaacaa attggctcca
    60541 gctgatacaa tggaagatga acttcacgat gcagtagttc ctcccattaa gaaaggatct
    60601 tgaaaatgaa gattacagaa aaagattttg gccaaggtta tcacttgata accttggaga
    60661 acaaaaacaa gctagcactt tctatctctg atttaggtgc tagaatcgtc agtttaaaat
    60721 caaatgatcg agaattagtt ttgggatttg acaccgctga agaatatatc gaaaaagatc
    60781 cgtatatcgg tgcttctatc ggtcgcaccg cagggagaat cgaaaatggc cgcttttctc
    60841 tcaatggaaa aacttaccaa ttagccacag atccaaaaac tggacataat ctgcatggtg
    60901 gtgctcctgg atttgaatta aaaaaatggt cttatgtcat tttgaatggc gaaaatgaag
    60961 cttctgtcat ttttactact acaagtcccg acggagaaca tggttttcca ggtacaatgg
    61021 atgtggaaat ccgctatact ttgacaaaag ataatatctg gcgagtgacc tctcgcggca
    61081 ccagtgacca agatacttta ttcaatccga caaatcatgt ttacttcaac ttgacgggag
    61141 atgcttctca gtcaatcgat caacatgagc tttggctcaa tagcgaagct tatgctcctt
    61201 tgcgaacaga tagtattcct ataggggtaa aagaaaatgc tgcaggaagt gctttcgatt
    61261 ttcaaatacc taaaaaatta gcgtccgttt ttgctagtga tttggatcaa aaaaatcttg
    61321 ttgacggaat cgatcatcca tttttcttaa aagaaaccgg cttgggtaaa gaagccgctc
    61381 gcttgactag ccccgataaa agaatccaag tagatattgc tacagatgct tctagtgtgg
    61441 ttattttcac tgcaaatttc ggcacagaaa caccagaaat gcgcaaccga aaactggcac
    61501 atcacggtgg catcacattt gagactcaaa cagctccagg tgcagagcgc ttctcagcat
    61561 ttggttcgat tcatttaaaa gccggatcag tatttgaaac agtaacagaa ttcaaaatca
    61621 aaacaagaaa ggaataactc atcatggaaa tcaaaagaag cttaaacaca aaatttgccg
    61681 aaacattcgg acctaaaaaa actacacaat acttctcacc aggacgcatc aatttgattg
    61741 gtgaacacac agattacaat ggaggccatg tcttccccgc ttccatcact tatggaacgc
    61801 aaggtgtagc agcacctcga gacgataata aaatattagt ttattccaca aactttgacg
    61861 atatcggtgt cattagtttt actttagatg agctagaata tgataaaaaa gcaaactggg
    61921 caaattacgt aaaaggaatg gtccttaaat taaaagaagc tggacactca atcgatcatg
    61981 ggtttgagtt attagtggaa ggaacaatcc ctaatggtgc aggactttcc agtagcgctt
    62041 cattagaatt attagtaggt gttgtattag aagatctgtt tgatttaaaa acagatcgct
    62101 tggaactagt ccaaacaggt aaaaaagtag aaaatgaatt catcggtgtc aattcaggta
    62161 tcatggatca gtttgccatc ggcttcggtg agctagatca tgcaatctta ttagatacaa
    62221 atacgctgaa atacgaaatg gttccagtga aactagatgg ttatgcaatc gtcatcatga
    62281 atacgaataa gcggcgggaa ttggcagatt caaaatacaa tgaacgccgt agcgaatgcg
    62341 aagaagcctt ggcacgtttg caaaaaactt tgcctatctc ttctttagga gacttagatg
    62401 aagaagaatt tgaatcaact attgaccaaa tcggcgatga tacattgata cgcagagctc
    62461 gccatgccgt ttctgaaaat cagcggactt tgaaggcaaa agctgaatta gaagctggga
    62521 acttagaagc atttggtcaa ctgttaaatg attctcacca ctctcttcgc tatgattatg
    62581 aagtaactgg gatcgaatta gatacactag tagatgctgc tcaaaaacaa gaaggtgtct
    62641 taggtgcacg aatgacagga gctggatttg gtggctgtgc gattgctctt gtcaaagaag
    62701 aaaatatacc tgccttcaaa aacaatgtct atgatgaata catgaaagtt gtcggctatg
    62761 ctccggaatt ttatgttgca cacattggaa atgggacaac aaagatcgat ccagaagctg
    62821 ttcatgtttg ataaaaaaat caacgatctc agctaaaaac aaatagataa aaataacctc
    62881 tcagcaattt ccaaatgtga gccttttgct cgtatttgaa aattgctgtt ttttattgac
    62941 acggaaagag aacgtgtgtt ctaattgtgt tgaagaggtg attgttatgt gggcgaatca
    63001 aaatccggta tttgtttatg aaaaagaacc ttcaagagat attttatgta ttgactgcaa
    63061 atctttttac gcctccgtag agtgcgttga aaaggatctt gatcccctta caactaaatt
    63121 agtagtcatg tcctatccgt ccgattcgac agaaacacgg ggtagcggat taatcttggc
    63181 ttccagtcct gcagcaaaaa aagcttacgg catcaccaat atcagccgtg ccagagatct
    63241 tcccttcccc tatccaagtg atttatatat tgttgcccca aggatggcct attatatgga
    63301 aaagaacaga gaaatcaatt ctatctataa aaaatatgca gacgaagcga accatcatgt
    63361 ctattccgtc gatgaaagct tccttgatgt aacaaatagc atggcactct ttggtaaaac
    63421 aaatgcgtac gacatggcta aactgatcca taaagatgtg tttgagcaga ccggaatcta
    63481 cacgactata ggaattggcg ataacccgct acttgctaaa ctagccttag acaatggctc
    63541 aaaagacgga aagcaaatgt ttgctgaatg gcgttatgaa gatgtaccag aaaagttatg
    63601 gaaaatctca gatatcactg acttttgggg aatcggcaaa cgaacagcaa aacgtctgaa
    63661 acaaatgggt atacattctg tttatgattt agctcacgcg gattattatc acttgaaaag
    63721 caagctaggg gtcatcggta ctcagcttta tgcccatgct tggggcatcg atcgaagttt
    63781 tttgggacaa gaatatacac caaaatcaaa aagtatcgga aatagtcaaa tcctagatag
    63841 agattataca agacgagaag aaatcgagat cgttatcaaa gaaatggccg accaagtcgg
    63901 cactcgcttg agacgatctg cagcaaaaac acaagtagtc agcttatggg taggatactc
    63961 tttaggctat cttgatccaa atggcaagac cgggtttcac cagcaaatga aaatcgaagc
    64021 gacaaatgct agcagagatc tagcagatgc tttattgacc attttccacc agcattacca
    64081 ctaccaggat attcggcgtg taggcgtgaa ctgttccgac ctcgtatacg cgacaggttt
    64141 acagcttaac ctttttgaag atcctgaaaa gcagataaat gaagcgaaag ttgattttgt
    64201 tgtcgatacg attagaaaaa aatacggctt tcaatcaatc atccatgcgc atagtctctt
    64261 gcaaggagga cgcgccatcg cacgtagttc tctcgttggc gggcatgcag gtggtatgag
    64321 tggattagaa ggtggaaaag atgagacgga cagcaaaaaa attcattgac tataacgagt
    64381 atcgagatcg gccatttgga ttaaaatggg ggactgcttt tgcgatggaa gaactagtca
    64441 acggtatcaa aaaaatgaag aagaagcacg caaacagcct cttcctaaac aacaaatgac
    64501 attgcaagaa atcgatagta tactgacaga agccttttta tctcgtaaag cagtaatcat
    64561 tcagctcaat ttaaaagatg aatttggaag actcttggat gaggtcgaag ggcacttcat
    64621 tggtgaagcc tatgaagatt actttatgat tgaccatcag ccgatattgt gggaagacgt
    64681 acggtatatt gagttaaagg aaacaaaaaa atggtttgtg acatgagagg ttgtgacaga
    64741 agtgatcaac ttcaaaaaat aagaaaggat ttttcgaaaa ttgcttctta aatttttgtg
    64801 gaattttggc ttatttttcg aagaagcaac ttctggaaca acgtatatta gatatcagag
    64861 gttgtgaaaa agtcgcttag ttttgagtaa caagccaagg cgctgatttc acaacctctg
    64921 tctatctatc catctattcg gtaagattca tgcatgacgt cttctttatt tagtatgtag
    64981 agcccttttt cttctgacca ttcaacataa atcagctgtt ttacatctgc atagccttct
    65041 ttcttcaagt tctccataag ccatgcttca gtcaattgat tggactgaag cactttatgc
    65101 tgaatttgcc cttcatctac aagcaggatc gataaagctt tgtcttcatc tgattttttc
    65161 aatacactca tttgaccggc attctctagg ataacatgtt tggcattttc aagaaaaaag
    65221 cattcttgct gtcttaagat tgctcgcaac tgctccatct cgatatggtt atttgtcaac
    65281 tctgtcatgt tgatgacgcc gtctttgatt agaacagatg gctcgccttt gacccaacga
    65341 ttgacctttt catttttttg gacgatacgt tcgataccat aaatcattac tgcccaaagc
    65401 gcaatggcaa ataggacatg ccctacatgg acattgtcat cataggttga ttcttctaag
    65461 atcccaccta aaacaagcgt atagactaaa tcaaacggtg tgatttctga catggatttc
    65521 ttcccaagca aacgtaccac taataataaa ccgatcaacc cgaacaccag tttcattgta
    65581 attgaaaaaa tcattttttc tccccctaac tatctgttaa ttaaattata tatgctttct
    65641 cacgaacacg catggaatat acaaggaaga aatgtatcct tttactaaaa aaaggactgt
    65701 gacactgtag tgacccccaa aagttagact tttttggagt ggagaaatcc actccttttt
    65761 tttatgctct aaaatgatat ttttacacaa ccgttacggt tgttttacga aattggacga
    65821 gactttgcca gtttaatttc tgttttatcc gttcattatt gtagtaatag atatagctat
    65881 cgatggctga ttttaattcc tcgaaacagt tataaacttt cccgtgaaaa atctcttgtt
    65941 ttaataagcc aaaaaaattc tccataagtg aattatctaa gcagttccct ttccgagaca
    66001 tgctttgaaa gattttgtac tccttcaatt tgtttctata agctttcatt tgatacgccc
    66061 aaccttgatc tgagtgaaac gtacgacgat agggacaatc gttagtggtc tggatagctt
    66121 cctccaaacc ttccattacc gctaatgcat tgggcttttc agagatacga taggataaaa
    66181 tctcactatt gaacatatcc ataaaaggat ctagatacaa tttcttttga cggataatgc
    66241 ctttggcatc cacttcatgg tatttaaatt cagttgtatc cgtcgtgatt ttctgatggc
    66301 agatactggt gtagaaacga cgatggatcc gatttttggc tactgctcca acttttcctc
    66361 gataagaatt gtagcgacgt gatttacgcg tgtatgccgt tacttttatt cctagcttct
    66421 tgatcaagcg ctgaactttc tttttattga tagagaaacc gcggtttctt agttcattgg
    66481 tcatccgtaa acagccataa tccttgtgct tctcccgaat tttaagcatt tccgcttcaa
    66541 tctcttgatc tggattttta cggtccaatc gcctttgcca tccaaataat catgaacgat
    66601 ctttaattta agttcaaaag tatatttaac cataaaaaat accgacctcc aaaagttaga
    66661 ttttttggtc taacttttgg gggtcggtac acaaatcgtg tcacagtccc ttttttgaaa
    66721 ataaacggtg ttcaaaagct tgcatctgcg gtaataagtc cagccaagtt aaaaacataa
    66781 gaagctgttt ttacttggct gttcttatta cttgatgcaa ggcagctttt tcacaacctc
    66841 ttttcaaata tttcgttttc ttattctaca aatggttctt ctcctaacgc ttgttgagcg
    66901 gctagattca acatgctcca ttgtcggtca aagcctggct ggaagaagaa gtcagcttct
    66961 gctagttctt ctaaagtcag tttgttttgg atcgctagtg ccaatacatt tccttgacct
    67021 gtaacatcat aggtagataa tactgctcct cctagcaatt gatgggtcat tggatccgag
    67081 aataattgga tggacacttt tggatttcct tgttcttctg gtacatattt tggtcgtaac
    67141 gtatcttcat aataagacac tcgtacatct actcctgctt ttgctgctgt aaagctattc
    67201 aatccgcttg ctgcaaaatg atagtcaaag acacttaatg cagaggaacc tacgactccg
    67261 ccgaacggtt tgtttggtac ttcttcaaaa agatgtttta ctacataacg tgcttctcta
    67321 cgtgcaacag tcgctaaagc gatcggcatt tttttgcgtg ctgggataga ataagcaagt
    67381 gtagcatctc ctactgcata cacatctgga agattcgttt gcaagtattc atttgtttgg
    67441 atccagccac gttcatctaa gtcgacgatc ccttttaacc attctgtatt tgccttcaca
    67501 ccggcagctt gaatgactag atctgctggg atttcttttt cacttgtttt gattgcctca
    67561 acttttccgt taccagtaaa agcttcgatt tttgcatttg tcaaaacttc aataccgttt
    67621 tcatttaggt gttgttctaa aatatctgtc atctcagcat ctaaataagt gcctaaaggt
    67681 ctatcgatca catcaagcaa tacgacttct ttcccagctt ttcggcttgc ttctgctgct
    67741 tcgatcccaa tgtaacccgc acctacgaca gcgatttttt taatagccgg atcatttaat
    67801 ttttctttga ttttggttgc ccaattgtat ccacgcatca agtagacatt ttctagatct
    67861 gctcctggta ctggcagatt gtttggtttg acaccagaac tcaaaatcaa tttgtcataa
    67921 ggatattctt ttgtttcgtt atctgatgcg ctagtaaccg ttactgtttt tttatcggca
    67981 tcgatcgttt ttacttcgtg attatttaaa atatgaacat tggattgcgg aaagttttct
    68041 ggtctgaaat tacgtacatc atttacatct gttaccttgt cttctaaata cagctccata
    68101 ccacatgaca tgaaggaaat atagtctcct gcttcaaata gcgtgatatc tacattttca
    68161 ccgtatcgtg tcaatagttc caagatcgat tgatgccctc catgagatgc acctacaatg
    68221 acaactttga ttttttccat tgatgaaacc tccttgaata ttcttttaaa atcattctaa
    68281 acaatttaaa cttaacatta cattagttat gaatcaaaag aaacgcgttc atttgaacta
    68341 aatttaaaaa acaatcgtct ccttgcagaa tataccgcaa aattaacgat tgtttttttg
    68401 ataaaatcta tgaatcactg ttctgttaaa aagtctttga tccgtttgca ggcctcatgt
    68461 aatttttcca tgctgctagc ataagaaatc cggatataac cttctccttc tgggccaaat
    68521 gcaattcctg ggatacacgc aacttttgcc tgttccgcta attctacaca aaactgccaa
    68581 gagtcagttt gaatggcagc tgggagtttg caaaacaagt aaaatgcacc gtttggctgg
    68641 gtcacttcaa atcctaaagg cgccaattgc tggaccagat agtctcttcg tgtttgatat
    68701 tgctctttca tattctcgct atctttcacc ccatttatta gcgcttcagc cgcagctttt
    68761 tgcgacatcg ttgtagcggc agtcaccaga tattgatgga ctttaattag ttcgtcaata
    68821 agggcttttt gagcaaaaat aaagcccact cgccaaccag tcatcgcatg tgacttggat
    68881 aacccgctta tcacgattgt ttgttctgga agataagtag ctatggacat atgcgttcca
    68941 ctataagtca attcagcata tacttcatca cttataacaa ataccggata tttttttaaa
    69001 acctcagcga ttgctttcaa ttgttttgca gacaatgtcg tcccagtagg attgttagga
    69061 tagtttaaaa tgactgcttt gacagctgat ccatattgtt caaatgctcg ttcaaattgt
    69121 tccggctgac agacaaaccc tgtgtcagaa gtatctatcg ggatcaattc agcaccagct
    69181 agttccacta atggctgata tcctgaataa gcaggcgctg gaataagaac cttgtctcct
    69241 tcttccaaaa cagataacaa cgcagaggca atcgcttccg ttgcaccaac tgtcgtcaaa
    69301 acttccgttt gcggatcata agttaatcca taacgctgtt gctgaaatat acaagccgct
    69361 tcccgcagtt cctctaatcc tctcattccg gtatagtgag aaaagtcttc ttcaatcgct
    69421 gctattcctg cttgcttgac atgttcagga gttggaaaat ccggttctcc cagggtgagt
    69481 ttgatcacat ctggtatcga cgatatttgc tgatcgaatt gtcgtatttt tgatacttct
    69541 attctagaaa gcctaggact aaatcgattg gataaattca tcattattta ctcgctcctc
    69601 ttttgaactg ctatcgattt ttacaagaag attttctgta aaaagaatcg ttttgtacca
    69661 ttctacacca ttttctaaaa catccatatc aaaatcgaac gtcggatcat gtaaagcagg
    69721 ggtatcaccg acacctaaaa agaaaaatac acatggatat tgttgcgtat atatgccaaa
    69781 atcttcggct tgaagaactg gagcatctaa ttcatgtagt ttgtttgaat ctaacagtgc
    69841 tgcatataag cctctatggt tgattacagc tggatagccg tcattatagc gaaaagaaat
    69901 agtagctgta gagtgaaact gttctgctaa atggtctaat cccctcttca aataattttg
    69961 catatctgaa ctgtaagaac ggatacttcc ggatacaata acttctccag ctaaaacatt
    70021 tcggatcgtt ccaccttgga tttggccaaa tttcattaga tgattccact ctgccggtaa
    70081 ttgccggtca aaatcataaa cattcttcaa aaatcgtaca gctgcctcca gactatcgat
    70141 tcctttttca ctagaagcaa tatgtgcaga ctggccttta atgataatgt ctgtttcact
    70201 gctttgtgcc atcaaaggtc ctggtcgaga gaaaacttct cccttagcta gtccaggcca
    70261 tagatgaaag ccaaatattg cttgtacttc atatttatcc aacaatcctg actgaataac
    70321 gctttgtgcg ccaccggaaa tttcttctga tggctgatag atcagtacta cattataaat
    70381 aagatcctca atgtgtttag ttataaagtc agtcaaccct aaaagcatcg ccatatgtcc
    70441 gtcatgacca catgcatgca tataaccttc atgcttagaa gcaaaagaga gattcgtttc
    70501 ttctttgact ggcagtgcat cgatatctgt tcgaaaagca atcgtttctt cttgctgatt
    70561 atcaaaataa aggagtagtc ctgtttcgcc gatttcatgg ataacggcct cgctgttttt
    70621 tacttgttca tataaatatt tttttgtctt gaattcatga aacccgattt caggaatttg
    70681 atggagtgcc attcgatatt ctttcagcat ccgtattcac catcccttca tagataccct
    70741 tcactactgt ttcatattga ttattcggta caccgatgat cagattcaac tcctgtgctc
    70801 cttgtgataa gatcgatgtg cgaatttcta atttgttcaa tatcgataac actttcccag
    70861 ataatccaat caattcttta tgcggacccc caacgacaga gatcaaagct aagtcttcga
    70921 tcacgacgat ttcttccacg ccaagtgttt cttttaattc tttcgtgatg agaaataact
    70981 tatcggcaat cgcttctgct gagacgatca ctccgatatt gtcgatccct gaaggaatat
    71041 gttcgattga tatctcatgt ttagcaaaaa tgctcattgc tttccagata aacccgattt
    71101 catcggacat atgacgtttg aataaagtga tggaaagaaa atccttgcgt ccagctattc
    71161 ctgtcagtcc attttccttt tctatttcgt gattcgatat ttttgtccct tgagcgaaag
    71221 gatcattggt attttttatt tggatgggga tatcagcctc ttgaactggg aaaacagctg
    71281 cttcatgaag aacgttagcc cccatataag ccatctctct cagttctcta aaagagattt
    71341 cctcgatgat tttaggatga tcgatgattc gcggatcagc catcatgatt cctgaaacat
    71401 ccgtccaatt ctcataactt tccgcttgca acatcttcgc cagtatcgcg ccggtaatgt
    71461 cgcttccccc acggcccaat aatttttctt ttcctgcagg gttcgccccg taaaaccctg
    71521 gaacaaccat tttcggatat cttttaaatg cttttgtgat ttcctgtgct gttttttcct
    71581 gatcgatttt cccatcatag tcaaagaaaa tcaggtcttt tgcgtcaata aactggtaac
    71641 caagatactc agccatcagt tgagcagtca gatattcacc tctacttact aaataatctt
    71701 cagttatctt ttcgtcttcc agttgtttct gtatgcgtgc tagttcatct tcaataggta
    71761 actgcagggc aagaacatct cttacttgaa tgaaacgatc acaaatcctt ttccaaaggg
    71821 gtgtccaatc aacatgatgc tgaaggtaag cataaatcaa ataaagtaag tctgttactt
    71881 tgtcatcatc tgacgtacgt ttgcctaaag cagaaacaac gatcacttgg cgattgggat
    71941 ctgaaagaac aattgtacgg acttttttga attgctcttc atgagacatc gatgttccgc
    72001 cgaatttcgc tactttcagc gtattactca tcttgccacc tccgctattt tctgcattaa
    72061 agctgcagca atctgtacgg cattcgcagc ggctcctttg cgtatgttat cggcagtcgt
    72121 atagaacaac aatccgttct ctaaagaagt gtcttttctg atcctcccaa cataaaccat
    72181 atctgttcct ctagcaagaa tgctcgtcgg atatttatgt gctgctagat catcttctaa
    72241 ttgaatccct ggaaactggc tgagacactc tctaatttct tccgtcgtaa actctttttg
    72301 cagttgaact gccacagaga ctccatgtcc attttcgatt ggcacacgta cgcaggttgc
    72361 tgatactggc aaagcaggct gatgcagaat cttacggttc tcatttatca ttttctgttc
    72421 ttcttttgta tacccatttt ccaaaggctg atcgatttcc ggaatcaccg tttggctaat
    72481 attataagga tacaaaactg gttcctcgtc attttgcgta cgatgaagat cttcaatgcc
    72541 tttttgcccg gaaccagaaa ctgcttgata ggtcgaataa ttgactcgct ggataccaaa
    72601 ggcttcttgt aaagctttaa gcggaagtac cgcttgaatg gttgaacaat ttggatttgc
    72661 aatcagccga ttcaatgtat agtcttccag attgacctct ggaacgatta atggaatatc
    72721 cgaatcttcc cgccatgctg aggaattatc tatgacaaat agtccctgac ttcgtgcgat
    72781 tggcgcaaac tctttggata cagaagcacc tgcagaaaat agcgctaaat catatccttc
    72841 aaatgaagtt tctgtcaatt cctgtacagt gtgtttcttt ttcttgaata cacaaacttt
    72901 accggctgaa cgttttgaag ctaataattt caatcgtcct actggaaaat catattcttc
    72961 taacactttg agcatcgttt ggccgactaa tccggttgcc cccacaatac aggtatctat
    73021 ttcgcgcatt tttctccatc tcctctataa ttcgcgtaac gcagcaatca atgctgtatt
    73081 ttcttttgtc tgatcatcac ttatttttaa tacacgtgca ggtattccac cgacaacagt
    73141 ttctgctgcc acatcttcta aaaccactgc tcctgctgcc acgactgcat cttttccgat
    73201 atggacacct tcaacaatca ctgcattcgc acctaccagt acgccgtctt ctacgataac
    73261 cggtttggcc gatgctggtt cgatcacacc tgctaagaca gctcctgcac caatatggca
    73321 gtttttgccc actgtcgctc gtccacctaa gacagcaccc atatcaatca tcgtgttttc
    73381 accgattact gcaccaatat tgataatcgc tcccatcatg atcacggcat tgttgccgat
    73441 tgacacttga tctctgataa tagcacctgg ttcgatacga gcattaattt ttttaatatc
    73501 tagcaaagga actgcgctgt atcttgcagc agtttcgatt tcgatttcaa tgattttttc
    73561 tgattcagct tctaaaaccg gagcaatcgt ctcatagtca ccaaaaacaa cgaagctgct
    73621 tccttcacca aaaactttac tttctttaaa ttcgattggc tcggttacat tcagatagac
    73681 tttcacaggc gtctttttag atgccgtttg aatgtagcgg atgatttctt gagcttccac
    73741 gttttttaca cccctttgtc cattaataaa tcttctaatt tgtaataacc tggtgactga
    73801 tctttcaacc agtccgctgc ttctactgcg cctgcagcaa agattcgatt agaaaaagat
    73861 tcatgtttga tcgtaaatat ttcatcagta gcagcaaaca atacatcatg ttcaccaggt
    73921 aaagaacctg cacgaatact gtgtacacca atttggtttt cttctctttt tttcccttgc
    73981 cagttgtata cgggctgcag cttttgcact tcttggattg tttgaagcag catatttgca
    74041 gtaccagatg gtgcatcttt tttacgacta tgatgttttt caatcagttc gatctgccag
    74101 ccttgcaaca ttgcagctgc ttgcttgatt agttgattca tcaccatgat gccgaacgaa
    74161 aaattagcac tatacatcac aggtacatgt ttggcatgtt cttttatttc ttggaactgt
    74221 gattgtgtat aacctgtcgt gccaatgact aacggaagtt gatatgataa gctatacgaa
    74281 aggattttct ctaaattatc tggatgagaa aaatcaataa gcagttctgg cagttccgta
    74341 aaagcaagcg tagttgcttc tttaggtgcc gtccgtaaaa gaaggaccgt atctcctctt
    74401 tcttgtgcaa cttcttcgac tctatttccc atctgtccac tgccaatcag tccgattttc
    74461 atttttcaac cgtcctttct tttaacaaaa gctgagctaa ttgtttcttt ttctcttttg
    74521 aaagagaaac taaaggcagg cgtagaggtc cggaaggtaa gtctaataat gacatggctg
    74581 ctttgacagg aataggattg gtctcactga acaaggcatg gatcagttca agtgcattta
    74641 attgcttttc ttttgcttgc tgccatcttc cgtcctgcgt atcttttact aattcatgga
    74701 ctattttagg ttggatattc gcccatacac ttattacacc gcttcctcct acactcatta
    74761 ctggtaggat caaatcatca tttccactgt aaaggaagaa ttcttctcca atcaaccgag
    74821 cggcatccat cacataagcc atatctcctg aggcttcttt caaaccaatg atattaggat
    74881 gttctgctaa atttactaat acatggattg gaatcgacat gcctgttcta gaaggaacat
    74941 tatacaaaat aagcggaatc ggtgaggctt ctgcaattgc tgtaaaatga gcagctagtc
    75001 ctgcatcact tgttttgttg tagtaagggg tgatgaccaa aagtgcgtct gcccccaaag
    75061 aagcaaaatg cttcgctttt tcaatcgttt ctttcgtatt attcgttcca gctcctgcaa
    75121 tcaccggcac acggccagcc actttcttta ctgtcagttg aaggatctgc tcctcctcag
    75181 attgtgtgag cgtagaagat tctcctgttg tacctaaaac taataatcca tctgtttgtt
    75241 catctaaatg gaattggatc aatttttcca aaccagcata atctactgat ccctcttcat
    75301 tcatcggcgt gatcagcgcc acgatcgaac cttttatcat cttcattcct ccaatattct
    75361 tgtccattca tgaactgctg ggccagccat ttgtatcgca tcctcaccag agatcatcaa
    75421 gtctccttgt tccaagtgaa caactacctg atctgctgaa attttcccag tttccttagc
    75481 aaccacataa gaggcacagc aacctgtacc gcaagcaagt gtccaaccta ctcctctttc
    75541 ataggtacgg acgatcaatt cttgttcatt gactacttct acaaagttga cattggtttt
    75601 ttctttgaac aaaggatgat gacagatcag ttcacctctt tttctccaga cttcctcacg
    75661 cgcattttca acaaaaacaa cggtatgaat cgttcctaaa aataaactag tgatcgttac
    75721 tttttcacca ttgatctcta tttcctggtt ttttattgga taagtttgat tagctttgat
    75781 tttttccgga gaatcatctg gcactcccat atctacgctg caataaaaag gtgagggatc
    75841 caacaccgta atttttcttg tgcctgctaa tgtctctacg ttgaattcct gttcattcat
    75901 catgttctga tcatttacgt atcgagcaaa acagcgaatg ccatttccgc acatcggcgc
    75961 gcggctgcca tctttgttgt aatagatcat ttccaacgga tcgattttta cggcgatcaa
    76021 tccgtctata tcatattttt cttttgtgca taaagaagcg gctaattgac tataatctgt
    76081 gtcttctgcg taatctaata agataaaatc atttccgcag ccattatatt tatgaaggat
    76141 cataggaaac ttcccccctt agtagatcag aaaatgcttg tctgcgaaca atcagtcttg
    76201 attggccatt attgacaaaa acaactgctg gtcgtgtcgc tcgattgtag ttgctgctca
    76261 tcgagtatcc ataagctccc gtggaataaa cagctaaaag atcacccgat tctgctttcg
    76321 gcaaataagc ttctttaatt accacatcgc ctgattcaca catttttcct gcaatcgtca
    76381 ctttctctgt tttttcttcc gacagttttg ctgctaaatc acaatcgtat tctgcctgat
    76441 acagagctgg acggatatta tctgtcatcc cgccatctac aaagtaatat tttttatgtg
    76501 gtgtcttttt gatgaaacca atcgtgtata gtgttgtacc agcttctcca acaatacttc
    76561 tacccggttc gatcagtaat tgacgaatgg tcagttttcg tttttccaaa gcttcttccg
    76621 tatattcaat cagttttttt agtgcctctt cgactgggat cggctgatca ttttgtgtat
    76681 aacgaatccc aaaccctccg ccaagattca aggaaagagg ttggtcaaaa tcggttaagt
    76741 aattcgtcaa tttgtcgatt tcagctaacc aagcagtaac atcaaaaatt tgtgacccga
    76801 tatgagcatg aaatccttct aactctagat agctgctttc ttttatcaac tgaagggctt
    76861 gttggcactc tttactttca tagctcatgc cgaattttga atcaatatgc gcagtgacga
    76921 tatattcatg agtatgtgct tctactccga cattcaaccg aagcatgata cagatttttt
    76981 ttaaatagcg ttggcttaat tctgctaata attcaacttc catcaaatta tcggcaacaa
    77041 tatgcaacaa atcattttca agtgcataga tcagttcctc tggcgtcttg ttgttaccat
    77101 ggaagtagat agtatctcta ggaaaatcag cttgcaaagc ggtatagatt tccccaccac
    77161 tgacaacatc tagcccgaat ccatattcac gaactaaatg cagcatctca atcgtttgga
    77221 atgctttaga ggcgtaaagc acttttgtat caaaacgttt cgatacaaag cctcgcttga
    77281 atgacagcat cgtttgtctc atcttttctt catcatacac atacagaggt gtcttatact
    77341 tttctgccaa tgtgcttgct gtgactccgc caattatcaa ttctgtcatt ttttcattcc
    77401 tcttttcact ttttttccgt ttttattgga atttcatttg ataaagattt gaattttatt
    77461 ggaataatat taaacaaaat tttctgaatt gtctagtata tttcgaaata tttttttaaa
    77521 aataagaata aaaatggtta taaaaacaaa atacccgaac tattagaagc tatttataca
    77581 taaaaacata aattcaacac tatttagtga tgaaaaaata ttttcgcttc aatatagttc
    77641 ggaatagttc ggatattttc aattttatga atcatttcaa tttcttcata aataagtgca
    77701 gttttttctt tctctagctt ttgccattca tctttcaaaa tataaataaa atcaaatact
    77761 tttttatttt tcaatttgct gattccacaa aaaacctctt gaaaggcaga tccgatgatc
    77821 tgcctttcaa gaggtcaagc tctgttttca aattcttatc aatccgttag cgaattattt
    77881 tgacgcacgt tttacataag aaccttctgc tgtattgatt tccaacaatt catctgtttc
    77941 gatgaagtca ggaacattga ctactaagcc agtttccatt gtcgctggct tacctgaacc
    78001 agtgactgtt gcacctttga tggaaggttg tgtttccaca acacgtaata cgacagttgt
    78061 tggtaattga acgccgatga cttcgctacc gaaaaattgg atttttactt ccatgttttc
    78121 taggatatat ttcatttctt cttctactgt ttctacagga atttcatatt gctcatatgt
    78181 ttccaaatcc atgaagaaag ctgtatcatc catcgtgtac aaatattgta caggttttgt
    78241 gtcgatatgt gctttttcaa atttttcttc tggtctgaac gttgtgtcgt atgttgcacc
    78301 tgaacggaca tctttcagtt tcatacgcat aactgtattt cctttaccag gtttatggtg
    78361 gcttgcatcc agaactttaa tcagtttgcc gtcttgtacg aaagtcatac cggctcttaa
    78421 atcacttgct gagatcatta tttttgctcc ttcttttctt tatcataatc aactttattg
    78481 tatcagacaa ccggccagaa aactagagta ttctctatct tttaaggtct catgcctatt
    78541 tgtcagaaaa ataaacacgg gtaaacgatg ctatcgaaat atttttttct ttcaagcctt
    78601 tgacttttat tttttttcat gctatcgtta ataaaaattt tacttggagg gatacacatg
    78661 acagtcagca accaaaaaca attgaatcgt tgctgttgta tggtgcataa aagcagcata
    78721 ctatttgtca tgtctattaa ttgcccttca tagaatgagt accaggtaac ttatcttttt
    78781 atctactcaa tttttctcta tgtctttcaa ggagctgatt taatagtcag ctcctttttt
    78841 tattatcttt ttttaggagg cccacatgat tatctcgaca attacagaag ctattggttc
    78901 cacaccgctt tatcgctttg acaatcagca ctattctatg cctaaaggtt ctgctattta
    78961 tgcaaaacta gaatatttaa atcctggagg aagtatcaaa gatagattgg gcagttattt
    79021 gatcaaagaa ggatttgccc aaggaaaaat caatgaaaaa acgacgatca tcgaaccaac
    79081 tgccggaaat acaggaattg ggatcgctct ggccgcactc acatacaaat taagaacggt
    79141 ttttgttgtt ccagagaagt ttagcttaga aaaacaacaa ttgatgaaag cattgggagc
    79201 aaagatcatc cattctccta gtgagcaagg aatcatcggt gccatcagca aaagtaaaaa
    79261 acttgcagag gagatatcaa acagctatct gcctcttcaa tttgaaaata aagataatcc
    79321 tgctgcttac tatcatacgt tagggccaga aatctttcat gaaatcaaag aaaatatcca
    79381 tagttttgta gcaggaattg ggagtggtgg aacctttgct ggaacttcta cctttttgaa
    79441 agaaaaatat cccgacattc gaataatcgg tgtagaacca gagggctctg tgttaaatgg
    79501 tggtgatcca gcccctcacg aaattgaggg tatcggtgta gaattcattc cgccgtttct
    79561 ttctcccctt tcaatcaatc agatcgagac aatctctgat gtagaagggt ttaactatac
    79621 taggcagtta gcacgagaac aaggactatt agttggaagt tcgagtggtg cggcttttgc
    79681 agcagcatta agagaaattc gtcgtttacc acctggtcat cgtgtcgtta cgatttttcc
    79741 agacgcagca gaccgctatt tatcaaaaaa catttactta taggaggaaa gaaacatgca
    79801 cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag gagctgtcag
    79861 tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc ctaaacaata
    79921 tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga ttgctgattt
    79981 ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc atgccgtttt
    80041 ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg gaggaacatt
    80101 ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca tagatacgag
    80161 taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt atcttgaaac
    80221 acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc tggctcatca
    80281 acatggattg atagttattg ctgacaatac atttgctact ccttattttc aaagaccttt
    80341 agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg gacacagcga
    80401 tgtagtagct ggtttagtga cttctaacca aaagatctag ccgatcagat cggattttac
    80461 caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca gcgcggaatc
    80521 aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt agctgatttt
    80581 ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga tcatgaactc
    80641 catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt tactttaaaa
    80701 aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt agcagaaagt
    80761 ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc gtctattcca
    80821 aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc tgtaggaatt
    80881 gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat caagaactag
    80941 aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg ctacaccttg
    81001 gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac taaataattc
    81061 tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa atgagaatga
    81121 atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta cgtgtagctg
    81181 atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc tttaaacaac
    81241 caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg actgccgaac
    81301 taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc caaacaggct
    81361 tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt cttgaagcaa
    81421 caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca caatgtggct
    81481 gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt ttagcaaaac
    81541 gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat gagaagcaat
    81601 atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag ttctaaacgg
    81661 gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat agaaaggaga
    81721 aaactttatg tctatttata attttgaaac tactttagaa gacggcactg tctattcatt
    81781 gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat gcggtctagc
    81841 acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag gtctggttat
    81901 cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg aagcagcaga
    81961 agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg acgtaaatgg
    82021 agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta ctgtcactaa
    82081 agcaataaaa tggaatttta gtaaattttt gattgataga aatggcaaag taatcgaacg
    82141 atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg ttctttaact
    82201 taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgccttt gcgtggcgac
    82261 gtcctactct cacaaggggc aacccctcac tacaatcggc gctaagaagc ttaacttctg
    82321 tgttcggcat ggttacaggt gtatccttct cgctatcgcc accacactgt ggtgttatct
    82381 tttattgagt aaattttgtt cactcaaaac tggatttgaa gtca
//
LOCUS       DACCCS010000004        77359 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.004, whole genome shotgun sequence.
ACCESSION   DACCCS010000004 DACCCS010000000
VERSION     DACCCS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.004"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(524..751)
                     /locus_tag="IVK82_000260"
     CDS             complement(524..751)
                     /locus_tag="IVK82_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6391352.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            complement(760..1233)
                     /locus_tag="IVK82_000261"
     CDS             complement(760..1233)
                     /locus_tag="IVK82_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP6391353.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            2463..3029
                     /gene="amaP"
                     /locus_tag="IVK82_000262"
     CDS             2463..3029
                     /gene="amaP"
                     /locus_tag="IVK82_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP6391354.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            3042..3230
                     /locus_tag="IVK82_000263"
     CDS             3042..3230
                     /locus_tag="IVK82_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP6391355.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            3243..3800
                     /locus_tag="IVK82_000264"
     CDS             3243..3800
                     /locus_tag="IVK82_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6391356.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            3832..4074
                     /locus_tag="IVK82_000265"
     CDS             3832..4074
                     /locus_tag="IVK82_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6391357.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            4415..5629
                     /locus_tag="IVK82_000266"
     CDS             4415..5629
                     /locus_tag="IVK82_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP6391358.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            5660..5878
                     /locus_tag="IVK82_000267"
                     /pseudo
     CDS             5660..5878
                     /locus_tag="IVK82_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6297..6770
                     /gene="dfrF"
                     /locus_tag="IVK82_000268"
     CDS             6297..6770
                     /gene="dfrF"
                     /locus_tag="IVK82_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP6391359.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            6897..7553
                     /locus_tag="IVK82_000269"
     CDS             6897..7553
                     /locus_tag="IVK82_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP6391360.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            7735..8265
                     /locus_tag="IVK82_000270"
     CDS             7735..8265
                     /locus_tag="IVK82_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6391361.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            8811..9053
                     /locus_tag="IVK82_000271"
     CDS             8811..9053
                     /locus_tag="IVK82_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAP6391362.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            9050..9520
                     /locus_tag="IVK82_000272"
     CDS             9050..9520
                     /locus_tag="IVK82_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6391363.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            9648..10862
                     /locus_tag="IVK82_000273"
     CDS             9648..10862
                     /locus_tag="IVK82_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6391364.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(11572..11760)
                     /locus_tag="IVK82_000274"
     CDS             complement(11572..11760)
                     /locus_tag="IVK82_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391365.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            11851..12060
                     /locus_tag="IVK82_000275"
     CDS             11851..12060
                     /locus_tag="IVK82_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAP6391366.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            12172..13785
                     /locus_tag="IVK82_000276"
     CDS             12172..13785
                     /locus_tag="IVK82_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAP6391367.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            13820..15031
                     /locus_tag="IVK82_000277"
                     /pseudo
     CDS             13820..15031
                     /locus_tag="IVK82_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            15054..15980
                     /gene="glsA"
                     /locus_tag="IVK82_000278"
     CDS             15054..15980
                     /gene="glsA"
                     /locus_tag="IVK82_000278"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP6391368.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            16188..17648
                     /locus_tag="IVK82_000279"
     CDS             16188..17648
                     /locus_tag="IVK82_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391369.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            17869..19632
                     /locus_tag="IVK82_000280"
     CDS             17869..19632
                     /locus_tag="IVK82_000280"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP6391370.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            19716..20174
                     /locus_tag="IVK82_000281"
     CDS             19716..20174
                     /locus_tag="IVK82_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP6391371.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            20283..20966
                     /locus_tag="IVK82_000282"
     CDS             20283..20966
                     /locus_tag="IVK82_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP6391372.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            21113..21727
                     /locus_tag="IVK82_000283"
     CDS             21113..21727
                     /locus_tag="IVK82_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP6391373.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            21743..21949
                     /locus_tag="IVK82_000284"
     CDS             21743..21949
                     /locus_tag="IVK82_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391374.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            22490..23620
                     /locus_tag="IVK82_000285"
     CDS             22490..23620
                     /locus_tag="IVK82_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP6391375.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            23712..24467
                     /locus_tag="IVK82_000286"
     CDS             23712..24467
                     /locus_tag="IVK82_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP6391376.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            24525..26564
                     /locus_tag="IVK82_000287"
     CDS             24525..26564
                     /locus_tag="IVK82_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP6391377.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(26673..27824)
                     /locus_tag="IVK82_000288"
     CDS             complement(26673..27824)
                     /locus_tag="IVK82_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP6391378.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(27978..28508)
                     /locus_tag="IVK82_000289"
     CDS             complement(27978..28508)
                     /locus_tag="IVK82_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391379.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(28607..28957)
                     /locus_tag="IVK82_000290"
     CDS             complement(28607..28957)
                     /locus_tag="IVK82_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP6391380.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(29114..30643)
                     /gene="ahpF"
                     /locus_tag="IVK82_000291"
     CDS             complement(29114..30643)
                     /gene="ahpF"
                     /locus_tag="IVK82_000291"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP6391381.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(30653..31216)
                     /gene="ahpC"
                     /locus_tag="IVK82_000292"
     CDS             complement(30653..31216)
                     /gene="ahpC"
                     /locus_tag="IVK82_000292"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP6391382.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(31360..32022)
                     /locus_tag="IVK82_000293"
     CDS             complement(31360..32022)
                     /locus_tag="IVK82_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6391383.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            32206..33852
                     /locus_tag="IVK82_000294"
     CDS             32206..33852
                     /locus_tag="IVK82_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP6391384.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            33843..34925
                     /gene="uxuA"
                     /locus_tag="IVK82_000295"
     CDS             33843..34925
                     /gene="uxuA"
                     /locus_tag="IVK82_000295"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP6391385.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(35033..36586)
                     /locus_tag="IVK82_000296"
                     /pseudo
     CDS             complement(35033..36586)
                     /locus_tag="IVK82_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            36734..37875
                     /locus_tag="IVK82_000297"
                     /pseudo
     CDS             36734..37875
                     /locus_tag="IVK82_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(38001..39080)
                     /locus_tag="IVK82_000298"
     CDS             complement(38001..39080)
                     /locus_tag="IVK82_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6391386.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(39183..39623)
                     /locus_tag="IVK82_000299"
     CDS             complement(39183..39623)
                     /locus_tag="IVK82_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6391387.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(39680..40090)
                     /locus_tag="IVK82_000300"
     CDS             complement(39680..40090)
                     /locus_tag="IVK82_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP6391388.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(40270..40704)
                     /locus_tag="IVK82_000301"
     CDS             complement(40270..40704)
                     /locus_tag="IVK82_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP6391389.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(40804..41652)
                     /locus_tag="IVK82_000302"
     CDS             complement(40804..41652)
                     /locus_tag="IVK82_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6391390.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            42041..43807
                     /locus_tag="IVK82_000303"
     CDS             42041..43807
                     /locus_tag="IVK82_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP6391391.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            43909..45168
                     /locus_tag="IVK82_000304"
     CDS             43909..45168
                     /locus_tag="IVK82_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6391392.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            45315..46616
                     /locus_tag="IVK82_000305"
     CDS             45315..46616
                     /locus_tag="IVK82_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6391393.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            46619..47470
                     /locus_tag="IVK82_000306"
     CDS             46619..47470
                     /locus_tag="IVK82_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6391394.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            47483..48298
                     /locus_tag="IVK82_000307"
     CDS             47483..48298
                     /locus_tag="IVK82_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP6391395.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            48449..49615
                     /locus_tag="IVK82_000308"
     CDS             48449..49615
                     /locus_tag="IVK82_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6391396.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            49845..50492
                     /locus_tag="IVK82_000309"
     CDS             49845..50492
                     /locus_tag="IVK82_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP6391397.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            50576..51520
                     /locus_tag="IVK82_000310"
     CDS             50576..51520
                     /locus_tag="IVK82_000310"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP6391398.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            51655..53106
                     /locus_tag="IVK82_000311"
     CDS             51655..53106
                     /locus_tag="IVK82_000311"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP6391399.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            53127..53963
                     /locus_tag="IVK82_000312"
     CDS             53127..53963
                     /locus_tag="IVK82_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP6391400.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            53963..54622
                     /locus_tag="IVK82_000313"
     CDS             53963..54622
                     /locus_tag="IVK82_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6391401.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            54748..55437
                     /locus_tag="IVK82_000314"
     CDS             54748..55437
                     /locus_tag="IVK82_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391402.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            55443..56399
                     /locus_tag="IVK82_000315"
     CDS             55443..56399
                     /locus_tag="IVK82_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP6391403.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            56443..56955
                     /locus_tag="IVK82_000316"
     CDS             56443..56955
                     /locus_tag="IVK82_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6391404.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(57211..57759)
                     /locus_tag="IVK82_000317"
     CDS             complement(57211..57759)
                     /locus_tag="IVK82_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6391405.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(57848..59029)
                     /locus_tag="IVK82_000318"
     CDS             complement(57848..59029)
                     /locus_tag="IVK82_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP6391406.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(59265..60773)
                     /locus_tag="IVK82_000319"
     CDS             complement(59265..60773)
                     /locus_tag="IVK82_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP6391407.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            60932..61123
                     /locus_tag="IVK82_000320"
     CDS             60932..61123
                     /locus_tag="IVK82_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391408.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            61326..62243
                     /locus_tag="IVK82_000321"
     CDS             61326..62243
                     /locus_tag="IVK82_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP6391409.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            62236..63309
                     /locus_tag="IVK82_000322"
     CDS             62236..63309
                     /locus_tag="IVK82_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391410.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            63457..63975
                     /locus_tag="IVK82_000323"
     CDS             63457..63975
                     /locus_tag="IVK82_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391411.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            63956..64348
                     /locus_tag="IVK82_000324"
     CDS             63956..64348
                     /locus_tag="IVK82_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP6391412.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            64424..65359
                     /locus_tag="IVK82_000325"
     CDS             64424..65359
                     /locus_tag="IVK82_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP6391413.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            65492..66271
                     /locus_tag="IVK82_000326"
     CDS             65492..66271
                     /locus_tag="IVK82_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP6391414.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            66264..66581
                     /locus_tag="IVK82_000327"
     CDS             66264..66581
                     /locus_tag="IVK82_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP6391415.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            66825..68435
                     /locus_tag="IVK82_000328"
     CDS             66825..68435
                     /locus_tag="IVK82_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6391416.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            68592..69440
                     /locus_tag="IVK82_000329"
     CDS             68592..69440
                     /locus_tag="IVK82_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6391417.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            69671..70150
                     /locus_tag="IVK82_000330"
     CDS             69671..70150
                     /locus_tag="IVK82_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391418.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            70183..72411
                     /locus_tag="IVK82_000331"
     CDS             70183..72411
                     /locus_tag="IVK82_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP6391419.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            72540..74183
                     /locus_tag="IVK82_000332"
     CDS             72540..74183
                     /locus_tag="IVK82_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP6391420.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(74256..74801)
                     /locus_tag="IVK82_000333"
     CDS             complement(74256..74801)
                     /locus_tag="IVK82_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP6391421.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(74824..75309)
                     /locus_tag="IVK82_000334"
     CDS             complement(74824..75309)
                     /locus_tag="IVK82_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6391422.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(75299..76111)
                     /locus_tag="IVK82_000335"
     CDS             complement(75299..76111)
                     /locus_tag="IVK82_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP6391423.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(76250..>77359)
                     /locus_tag="IVK82_000336"
     CDS             complement(76250..>77359)
                     /locus_tag="IVK82_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP6391424.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        26028 a        13423 c        15201 g        22707 t
ORIGIN      
        1 cattattatc aaaataatgt taaaaataaa aaagaattgt ccatcactgt tattcaaatc
       61 attttatcaa aactacatag taaatgttaa aaagcaacat gacataatat atagaaggct
      121 ttacagtata tatgggagct tactaaaaaa gaatggagta agagttaaca caatgggcca
      181 ttagctgaag ttttaagcac ttcaaggtat gaaacagaat ggcctgtaat actacatgat
      241 tatttagact gctcaaactg ctatcagtct ggtggaatga ataaagtatt atactaaaag
      301 cagatccaac tacaacgtaa caggaaacca gttgtatcag gaaaagtgat agtacatgga
      361 gaagaaaagg ccccctttgt atgagtcagt acatatctta tttatagata aaacggtatt
      421 caatcgaaat aaaaaatctg aataatagac gtaaataact tctaaaagtc tcacacatct
      481 atcattcaga ttatttttta tttactgttt gttctacttt attttattct tctggtatag
      541 tattcgagtc atttttatag gtcaacaaat cacctggttg acactccaaa acttggcaga
      601 ttttttcgag agtggaaaaa cggatggctt ttgcttttcc attttttaga atagagatgt
      661 ttgccatcgt aatgcccact ttctcactca attcagtcac actcattttt ttttgagcta
      721 atactacatc taaattaacg ataattgcca tgtcgcacct cacactgtca aatcatgatc
      781 tgtcttaagt aaaatcgcat gttcaagcag tttttccagc acgcttgtta atgtgtatac
      841 tgcaaaagga atgaatacga cagcgattcc taccaacatc aaacctggag catcgtctaa
      901 atccgctact ttcataaatt taggaagtaa gccaaaaaag gcgaaaccaa taaacaaaat
      961 ggttcgttcg atttttttta gtatgtttaa gctttgagtg gaaaaagctt ggttatcttc
     1021 gatttttttc aatagtttca ataagaaata agcaataacg atccctagga gaagggcaaa
     1081 gtaaatgaca gaaacaaata atgtggtctg ccaatcaaaa ttgaatcctt tttcgcttgt
     1141 agcgatctga gtataaaaaa gtacgccgaa caaaataaat acggcactta tacctaaaac
     1201 gacaaatttt aatagcaatg ttttaaattt catttttatc cctcttttat ctttgatagc
     1261 taaaatatag cagaataatt attgtaaaac aactattttt tatcgaaaaa caataaaaat
     1321 atatcgttta taaaaaatga tagagtatga aggtgtggag tgcatttaac agcttttttt
     1381 gattgtaaga aacatttaaa accagttatt ttagattcta tgtgtgtctc ttctataatg
     1441 ctagtttaga tgcctgggat tatattggac aatacagttg gaggattttt tattatctag
     1501 cgaagagaag tgtaagttaa cagaactgta tgagaaaaaa gacaactacg tagtagctaa
     1561 ccgattcatt aattttttca agttcatgtt tatttacgta tcagaatttt tcttattcaa
     1621 tggtttggtg taatcaccac ttatttgtcc tttattgctt atacaatgtt atacaaaagg
     1681 aaaatcaagc attgacgcat ggggtggtac tatctagctg aaatagctta ataagcgcaa
     1741 gtagatgcat tggattagtt tataaatcaa aagtgttaga ggtattaaga agaagtagaa
     1801 aaataaaatg gattgccgaa taaaaagtac cataaatcct aaaacatgtg ccaacagata
     1861 gatggcacat gtttattttt ttcgataatt ttgtaacaaa aatacgaaga tgaaaaagaa
     1921 tagagattcc cataatgtag tagtccaact gaattcttta ctgataccaa ataccatatt
     1981 tcctagattt gatacaatca aatagccaat cgttatgatc aatgaatagt acaatctttt
     2041 catcatagga catacctcgt ttttatttat aagaatagta taaaccaaag aagtgaataa
     2101 gaaaaatgat aatataaaaa taaagtaaga acgcattaaa gtaactaaag ttatttattg
     2161 tattttttaa ttctccgaaa aaatttaata aataaataga atcttgtaat aataactgag
     2221 cataatcttt gcaattttta atcttgaata atatccttta agtatagcca gcaatcaaaa
     2281 gtaaatagag aataagtaag caattgttat ttcagtatca gactggcaat aaaacgccgt
     2341 tatgatccag caatgtttgg tagcgcttta tttactgatg ctatgctaac tgttcgctat
     2401 ctatactgtg attgtcggta gagttattta cagggtaaaa ataagtttat aggaggtgga
     2461 aaatgaataa ggggttaaaa ataatcggtg ttatcatgag tttactgcta ctttcagtat
     2521 ttctagcgat tgcgttgatc aatgcgccgt atattatgcc tgaacaattg gaacgatttc
     2581 gcttctttac tttaacgaat tactacatgc aacaatatat tttctgggca gctgtagtat
     2641 ttgccgttct tgctattatc atcttgctag tcgttctttt ctatccaaag tcaagaggaa
     2701 ctttcgttat gaaacgggaa gatggcaaac taacaatcga caaaaaagcg atcgaagggt
     2761 tggtacgttc ccatttacac gaagaagagt tcattcattc acctaaagta cggatacgtt
     2821 caacgaaaaa tcgaatccat atccatgtca acggagactt gaaacgaact tcttcattag
     2881 tagggaaaac aggtgtattg atgcaagata tcgaagaaga agtaactaaa gtactcggaa
     2941 caaaagaaac ggtcaaagtg gcagtcacat acagcggtta tgaagaacaa gaagatacaa
     3001 gagactacaa gcactctcga gtggaatagg agggccaaat aatgaaagaa ctattttcag
     3061 catacaagct ccctattatc tttgggggtc ttggactact gctagctgtt ttatggttaa
     3121 cgattggatt tttcaagaca ttgttgatca taatcatgac agcaattggc gcaggaatcg
     3181 gtttttattt gaaacagtca ggaatagttg atcaattttt taaatcatag gaggaaacaa
     3241 agatggaaaa caaaaacatg aatacaacaa atggatcagt tttggcaaat gaaaaaggag
     3301 aaaaccgcat gatgaatggt gaattgactt atgaagacaa agtcatccaa aaaatcatcg
     3361 gttatgcatt gaaagatgta gatggtctat tgacattaga tggcggattt ttctcaaata
     3421 tggctgaaaa attagtcaac acagataacg taacagcagg tatcaataca gaagtaggta
     3481 agaaacaagt agctgtggat ctagatgtga tcgtagaata cggtaaagac atcgaagata
     3541 tttacaacca aatcaaagaa ttgatcagta ctgaagtaaa caatatgaca catttagatg
     3601 ttatcgaagt gaatgtgaat gttgcagaca tcaaaacaca agaagaatac caaaaagatc
     3661 aagaaacagt acaagacaaa gtaacagaag cagcaaaatc cacaggacaa tttgcttcac
     3721 gccaaacaga taaagcaaaa tctgctgtag gcaaaggtgc acaaaaagtc aaagaaaata
     3781 acgaaccacg tgtacaataa gaagtaagaa gtcagttagg aggaatttaa aatgggattt
     3841 atttgggcat taatcgtcgg cggtatcatt ggagcaattg caggagcaat cacgaataaa
     3901 ggatcatcaa tggggatcat cgcaaacatc attgctggtt tagtaggttc agcaattgga
     3961 caagctttac taggaacttg gggaccaaca attgcaggaa tggctatcat tccatcattg
     4021 atcggtgcaa tcattttagt attagtcgtt tcattctttg cacgtaaatt ataagcaaat
     4081 tgaagaaaaa cttatgttga aatgaagaac aaatttgaga accgtagcga cactcgctgc
     4141 ggttcttttt tatatatttt atatgaagta ttctttattc cttatgaaaa aatatcttta
     4201 aaaataaaaa aagacgaaaa taaacggttt tctataaaaa gaagaccgtt tattttttgt
     4261 ctttatttgt gtgaaaaacg aaaaagagca caacacatca agcttttatt aattgtctgt
     4321 cttttataaa tgcaagtatt aattcaaaaa aacagaagaa aaaaacaact acaccttctt
     4381 ttatactcgt aatcgtagaa aggaggcgaa aaaaatgaat acattaccta tcaatacagg
     4441 agatacagca ttcatgattt tatgtacagc aatggtttgc ctgatgactc ctggtctagc
     4501 ttttttttac ggcggattag cacggaagaa taatatacta acgatcatgt ctcaatcttt
     4561 tctctccgtt gggataacaa cgattatgtg ggtatttggt ggattcggat tggcctttgg
     4621 acgggatatc ggcggtgtta ttggaaaccc ggcagatttc tttttgatga gacatgttac
     4681 aagcttcccc aacatgtttc atggtgcaac gattccattt ttaatgttct ttctttacca
     4741 actgatgttt atcgtgatta ccgttccttt gatgacagga gcttttgcag ggagattgaa
     4801 tttaaaaggc tatattatac ttgttatttt ttggaatctg ctgatttatt tccctgtttg
     4861 ccactggata tggggtggcg gatttttgga tcagatggga tttagagatt tcgctggtgg
     4921 agcagtcatc cacacgacag ctggattcgg ctctttagca tgtatcctca cacttggtca
     4981 aagaaagatt cctttaggag aaagaagtca gcatagcagt cttatggcag ctgctatcgg
     5041 tacaggactg ctttggtttg gctggttcgg ttttaattcc ggtggtgcac ttcgagcaga
     5101 tgaacaagca gtcaatgcat ttggttcaac attcatcgct cttgcatttg cgatgattac
     5161 ttggctgatc attgcaaaag taaaaggaaa tggctttgat tttgtcgacg tactaacagg
     5221 atccgttgct ggattggcag ccattacgcc ttgtgccggt tatgtagaag caaaaagtgc
     5281 catgctgatt gggatcattg cgggtatcgt ctgtcatgct gctgttgatt ttcgtaaaaa
     5341 gaaacaatgg gatgatgcac ttgatgtgtg gggtgttcat ggcatgggcg gattcacagg
     5401 tacgattctg atagggatct ttgcatctaa cagccattta ctaacgaatc caagcagctg
     5461 gtacttctta gggattcaag tgattggtgt catcattact gccgtgtatg cctatgtgct
     5521 gacaacagtg attttaaaat cggcgatgca ctttacaacg atcactacta caaaagaaga
     5581 acaagaagaa ggattggact ttttcttctt tggcaacaaa ctcaattgaa acaaattcaa
     5641 atagaaaaga ggaagattca tgcaacaaag caaaacgaaa attcctgcaa aaaaattaac
     5701 gttctttggc tttttggcta tgaccatctc aatggtcgtt tcattatatg aatatcccac
     5761 attcgcgacc tcaggcttca gcttagtatt tttcttgtta ttaggcggtt tgctatggtt
     5821 tatccctgtg gcattgtgcg cagcagagat ggcaacggtg aaaggatggg aaaaaggtgt
     5881 ttcccaaaca aaaagatatt aagaaacagc ccatgtttac ctgcttgcaa acaaaagaat
     5941 attgctacta gactaagaga tactacataa gtgtgttggt atctcttttt tgttgccgtg
     6001 aaacagcaga ctatttcatt tctgcttgat taccttaaaa cttattagtc tatggtttgt
     6061 caagggcttg ttgcgtgaga aatgctgaat atcacccttt acaaaacata ggctgataag
     6121 taacttttca aaagcagaaa agaaatggca atgcttataa caatatgata taatgcagat
     6181 gaagacaaaa aaggaatttg tcgagctggt tagttcgaga taattttgag tttgttaaga
     6241 tgtggtgcac ttgatggaat cacaggtacg acatcatatt atggaggatg aaatagatga
     6301 taggtttgat tgttgcgagg tcaaagaata atgttatagg caagaatggt aatataccat
     6361 ggaaaataaa gggagaacaa aagcaattta gagagttaac aacgggtaat gtggttatta
     6421 tggggcgaaa gtcttatgaa gaaatcggtc atccgttgcc taatagaatg aatattgttg
     6481 tttccaccac aacagagtat caaggagata atttagtttc agttaaatca ttagaagatg
     6541 cattattatt ggctaaagga cgagatgtat acatatctgg tggatatgga ctatttaagg
     6601 aagctttgca aatagtagat aaaatgtata tcacagaagt agatttaaat attgaagatg
     6661 gagatacatt ctttccagaa tttgatatca atgattttga agttttgata ggggaaacac
     6721 ttggtgagga agtgaaatat acgagaacat tttatgtaag gaaaaaatga attgagtaaa
     6781 ttttggattt agggtgtttt cataaatata ttgcttttta tgcatatata aattgttgta
     6841 agacaaattc cagtttgtat ggggcgataa aatcagaatt tgagagagga ttgaatatga
     6901 ataaggaatg gtctgaactt aataaaacaa tgcaggcaca aataaaaaag aaagatactt
     6961 ataagagggg tattgatact ttacttactt tacgaagtca gttaatacaa accttagtat
     7021 ctttcaaaga agaattatgc agagaagatt ttaactcaat ccccttcata aacgctgatg
     7081 gttatcatag taagacgatt gcttattcta tttggcatat tttccgcatt gaagatatag
     7141 ttgtgcatac agtgataaat gaagatgaac aagtattttt tgcaggcaat tatcaagagc
     7201 gtatcaattc acctatcatt acaacaggaa atgaactcat gaaacagcag attgcagact
     7261 tttcaaaaca gcttaatctg gaagaattat atttatatat tttcgaagta tgggaaagca
     7321 ctgagaaaat gcttgaacgg ttatcttatg atgaattgaa aagaaaaata ccgaaagaaa
     7381 gaaaaggata cttagaatcg ttgaatgtag taaacgacaa tgaaaaagca atttggctga
     7441 ttgattattg gtgtaataaa gatatttgtg gactaattca aatgccgttt tcaagacatt
     7501 ggattatgca cacagaagcc tgtttgcgta taaagaacaa gatacattca taggcaaaaa
     7561 tttcagtttg tagaactaag gaaattggaa tttaaggagg aataaagtat ggttagtgat
     7621 tttataagag cagcatttcc atggatttta atgggtttat ttgtagcaat cagttgttcc
     7681 cttatgagta aaaaagagaa ataaattcct gtttgtattt ataaaagaga aaaaatgaga
     7741 aaagtagttt tatttattgc catgagcctt gacggatata ttgcggacgg taatggtgga
     7801 gtagcttggc taaatggtca tggaaatgac aatgaaaaca tcgacactta tacagaattt
     7861 accaaagata tagatactgt cttaatgggg tggaatactt ctcatcaggt tgttactgaa
     7921 ctttctccgc aagaatgggt atataataaa tttacaactt atgtattaac acataaagag
     7981 tgcaattctt cccaacaaat tcattttaca agtgaaaatc ctgttttatt attggaacgg
     8041 ctaaaacaag aagcaggaaa ggatatttgg atttgtggcg gagcaaatct tgttcagcag
     8101 ttggtaagca aaaatatgat tgataaatat tacatttctg ttattcctac tctgttagga
     8161 aatggggttc gtcttttggg caacatagac agggaaataa agttaaggct ttgtaaaaca
     8221 cagacttata acggaataac cgatttgatt tatatgcgta gataactaga gagaaacaga
     8281 aaagctgtta aaccgacgga gcgatccgac cacgttggcg ggaaaatcct taagattttt
     8341 ccgccatgct tgcaaggggg cggttcgctt cggcggttcg gcgagcttgc gagccgttca
     8401 aagcccccgt tatctaacaa ggggggcaca aaaaacaaga aacactatac actaacaagc
     8461 ggaaaacgct gtatttataa ggcaaaaccc catttttaca gtagtgatat aaattgtacc
     8521 tatatcacta tgaaaattga atagtaaata tgagaaagac agttgatttc aaaaaataag
     8581 aaattgattg tcgttttctt ttgcagaaat ttaagtgcag gcaattatgg tagttgtcgt
     8641 aattgctgtc agctcagtgt ttatcactga tggtgtgaaa gatttacggt tggagatgtt
     8701 caataagatt attggtgctt aattattttt attgtagtgc tgatatgaga atgttataat
     8761 tggggtataa aacatacaaa taaatttgct gaaaaatgag gtatcaaaat atggaaaatg
     8821 aattactgga aaacttagga agacttcaca caactgaact tggcgttatt agaattaaaa
     8881 agaacttatc tttgaatgta gaaaatgtaa ttgaatggtg caaagaaaaa attagccttt
     8941 ctaatgcgga aatcacaaga aaaggaaaaa actggtatat aacgattgat aattgtatta
     9001 taacggtaaa tgcgtacagt tataccgtta tcactgctca caaggtaaaa tgataaattt
     9061 ttctataaag gaattaaacg catttgcagt tattgggcag gaagtagaac tgactaatta
     9121 tcaaaaaaag aatattcaga ttagtacaca gttttggaga aagtttaaca gtagtttgaa
     9181 aaaagcgtat ctttcacaat caggaaattg ggtaaaatat gcttttatgg aaagaagaaa
     9241 tggaaaactt tattatttct gttctattcc ccaaagaacc attaccccag agggctttct
     9301 gtataaagaa ataccgtctt ataaatattt ggttgtggag catattggtg ctatggataa
     9361 aatatatgaa acttatggca agatttatga agaaattatt cctagtacat cgtatattcc
     9421 tataaaagat attatcctac actttgaaaa atacgattat cgttttcatt ggaatagtga
     9481 taattcagtt attgaaattt ggatacctat tcaagattag aagcaatttt atatccatac
     9541 acacaaagca gttagtctta ggattgactg ctttttctat acagattttt aatgacaggc
     9601 aattatcaat tatggtagtt gccttttttg attggaggaa ttgaagaatg aatgataaaa
     9661 actttattga agaactaaga caaaagcgtg aggaatacgg agtaacacaa acaaggcttg
     9721 ctgttgcctg tggtatcagc cgtgaatatt acaaccgcat tgaaaaagga aaacagccat
     9781 tgaatgacga attaagagaa atcatagaaa aacagattga gcgtttcaat ccgcaagaac
     9841 cactattctt attgattgat tactttcgtg tccgctttcc tactacggac gcattggcga
     9901 ttatccgtga tgtattacaa ctgaaatcta attacatgct ttatgaagat tatggaaaat
     9961 acggatatga aagcaaatat gtacttggcg acatcaatat catgtgttcc atgcaggagc
    10021 atttaggtgt tttattggaa ctgaaaggca aaggctgtcg gcaaatggaa tgttatctgc
    10081 tggcacagga acgctcatgg tatgacttca tgctggattg tatgacggct ggtggtgtga
    10141 tgaaacggct tgatttggct atcaatgata aagcaggaat attggatatt ccaaagttaa
    10201 aggaaaaata caaggctggc gaatgtatgt cctacttccg tatgcagaaa gattacagcg
    10261 gtacagaaaa atgcggtagc gatttaccaa agaatacagg agaaacctta tatctcggtt
    10321 caacaagtag cgaattatat atgtgtgctt atcagaaaaa ttatgagcag tatgtcaaga
    10381 atggcacaga aattgaagat acggagatta agaaccgttt tgaaatacgc atgaagaacg
    10441 aacgagccta ttatgcggtt gtagatttac tgacctatcg ggacgctgaa cgcaccgctt
    10501 tttctatcat caatcattat gtccgctttg ttgacagaga ggacgacaag ccaaaaagtc
    10561 aatggataac aaatgatgat tgggcatggt ttgtagggga aaacagagag ccgatacgct
    10621 taaccacaaa gccagagcct tacactttac aaaaagcgtt gcattggcta caaagacaag
    10681 tcgcaccaac cataaaaatg gtacaggcat tagacagaga aaatcgtaca acgatactga
    10741 aagatatgat tgagcaggca gaactaaaag acaagcataa acatttatta caattagaga
    10801 aatcaaccat agaagaacgt atagataccg ctgttccaca agagaatgac ggtatttttt
    10861 aatgtaattg aaaaatttca gatttagtca gcaaagagct gttttcattc cctatgtgaa
    10921 gtgaaagaag aaaaataaat tttttattaa atctgcaaca aaatggctac ttgcgtacag
    10981 atatggtgcg agaaaaggaa atctaaactt tttttgaaaa taggtcatta aatcggggct
    11041 tgctgtccct atatgaagtg aaagaagaaa aaagttttga aaaaatgacg gaaattcgtg
    11101 attagtggac agtcttctgt gaaaagagga aaaacatcat cattttgtat tcaaacaggc
    11161 attagcagtt gagtgttgat gtgaaaagat gaaaatactt tgttctcaac ttaacaattt
    11221 aaggtttccg ttccctatat ggtgcaaaga aaatatttca ttctattgtt ggcagtaacg
    11281 gtatggtttg ggtagttagg gtgtgaaaag gaaaatattc ttttttcatc atcaacttag
    11341 caaaatgaca ttttctatcc ctatatggtg catgaaaaga agttaaaatt tttccattta
    11401 aacttagcaa atggacgttt gctgtacaga tagtagtgaa aaaggagaaa atcaaccgtt
    11461 gattgccaaa atgaaccgtt ccatttgtac taataatgga aaaagttttg gattttggtt
    11521 acgtttctcc tcttttccaa cgcgatatat aggaaagacg atttttttct ttcaatcgtt
    11581 ctttgacaac tgaataaagc tattcaatac gtttgacaga cagctagccg ttgaaacaaa
    11641 tacgccacga cctttcccct gcaagagcga gcgaaaacct atttgtgttc ttgtaaaaga
    11701 tttgtcctct tttatcgcca tgacctgtac tgtcagataa tgatactccc gtacagccac
    11761 agcttgagca ttaagagcgt cgcacgcaat gggtacggct acataagaac tatgcagagg
    11821 tggaactcct gtgggctatg acaaaagccg ttgaattgct tagaaagatt ttacagaaag
    11881 ggggtatgtg ttattgataa tgctgtaaaa acaattcaaa agaaaaatgg caagtgtggc
    11941 attggcaaca gaggaaagac aaagattgta gtaaaagagc atttttccga aaagggcaaa
    12001 acaatggaag aacttctaac tgatgtcatg ctggaaaaag caaagcagac aatcgcataa
    12061 aatcggtgca gttgtaagaa atagattgaa tgacaaaaag tacccgtgtt atactttact
    12121 tgcaagcagg tattgtaaac acgggttagt tataaaggag gataactgac aatgaaacaa
    12181 cagatttaca atactgcact ttatcttagg ttaagccgag atgatgaatt acagggcgaa
    12241 agttccagca ttaccacaca gagaagtatg ttgcgtttat atgcaaaaga acatcatttg
    12301 aatgtcattg atgaatatat tgatgacggc tggtcgggaa caaattttga cagaccgagt
    12361 tttcaaagaa tgattgagga tatagaggca ggaaaaatca actgtgttgt aacgaaagac
    12421 ctttcccgtc ttggcagaaa ttatattatg acaggacaat atacagaatt gtatttccct
    12481 agtcataatg tccgctacat agcgattgat gacggtgtag acagcgaaaa aggcgaaagt
    12541 gagattgcac catttaagaa catcatcaat gaatgggtgg caagagatac aagccgtaaa
    12601 gtcaaatcag catttaagac aaagtttgcg gagggggctc attacggtgc ttatgctccg
    12661 ttaggatata agaaacaccc tgacatcaaa ggaaaactgt tggttgatga tgaaacaaaa
    12721 tggattattg aaaaaatctt ttccctagcc tatcaaggtt acggaagtgc aaaaatcaca
    12781 aagcagttaa gagcagaaaa agttccgaca gcgtcatggc taaattttac aagatacggt
    12841 acttttgcac atatctttga gggaaaaccc gaaagtaagc gttatgagtg gacgattgct
    12901 catgtcaagg cgatattgaa aagtgaggtt tatatcggaa acagtgttca caatatgcag
    12961 tctacggtat cgttcaagag caaaaagaaa gtgcgtaagc ccgaaagtga atggtttcga
    13021 gtagaaaaca ctcacgagcc gattattgac aaggaagtgt tctatcgtgt gcaggagcag
    13081 ataaaatcaa gacgcagaca gacaaaggaa aaggcaacgc cgatttttgc agggcttgtc
    13141 aagtgtgcgg attgtggttg gtctatgaga tttggaacga ataagacaaa taaaacgcct
    13201 tacagttatt atgcttgcag ttactacggg cagtttggca aaggtaattg ttctatgcac
    13261 tacattcgtt atgatgtgct gtatcaagcc gtattggaac gcttgcagta ttgggctaag
    13321 gcagtacagc aggacgaaga aaaggtattg aacaagatac agaaagctgg caatgcagag
    13381 agaatacggg aaaagaagaa aaaggcaagt acattgaaga aagccgagaa cagacaaaat
    13441 gagattgacc gtttatttgc gaaaatgtat gaagatagag cctgtgagaa gataacagag
    13501 cgaaattttg tgatgttgtc cagcaagtac caaaaagaac agatagaact ggaacagcag
    13561 ataacaagcc taagagaaga actaagtaaa atggaacagg atatgatagg tgctgaaaag
    13621 tggattgagt taatcaagga atattccgta ccaaaggaac tgacagcacc attattaaat
    13681 gccatgatag aaaaaatcct catacatgaa gcaacaacga atgaggataa cgaaagaata
    13741 caggaaattg agatatatta ccgatttatt ggaagagttg agtaatcaac aagagtaata
    13801 tttttaacta agggaaaccg gcggtgtcta tacttgggtc agtcgcacat tgggaaaacg
    13861 attcggtttt gccgcaatct ttttccaatg gtttgagata acagttggct acttgaccat
    13921 gctgtatttc ttaactggtg cattatccta tgcaacgggt attggtgcta tacaaaacaa
    13981 taaatatctg aaattggcaa tcctattgat tattttctgg gctattttac tctcacagtt
    14041 aaaaggaacc aaatacactt cattgattgc tcggatcggt tttgtagctg gtatcttgtt
    14101 gccagcatta gtcttgtttg gcttagggat ccattatgtc gcttcaggcg ctccactaca
    14161 aacgatactg tctatgaaaa cattgattcc tgatttttcg aaattgccta cgctggttgt
    14221 ttttgtttcc tttattttag catatatggg cgtagaaaca tctgccagtc atgcaaatga
    14281 aatggaaaac cctaaaaagg attatccatt agcaatgttt gtattggtta ttctggcaat
    14341 cattttagat acttttggcg gattgaccgt cgctactaca gtacctcaac atggtttgtc
    14401 tcttaataca ggggtgattc aatcgttaga attcctatta agacacattg atccaaatct
    14461 tgcatggatt gcgaaaattc ttgcgattct tgtttgttta ggtgtcatcg gagaaattgc
    14521 ttcttgggta acctctcctt caaaagcact tcacgtagca gcagaagaag gattacttcc
    14581 tgaatatttt gcaaaagaaa acacacacgg tgttcctgct catttgatga tcgcaaatgg
    14641 gatcgttgca acaatctggg cagccgtctt tacattaagc ggcgggggga ataatctttc
    14701 atttcttgca gcgatgtcat tgactgttgt aatctacctg atgttgtact ttatgttttt
    14761 cgcgggctat ttgaagctgg tttttaaaga tgacagtctt gaaagagcct atcaggttcc
    14821 aggcggaaaa gttgtaaaag taatcgttgc ttgtttaggt cttttgactt cactttttgc
    14881 tttttgtatc tcatttgttc cacctgctag tatcgcagct ggacagcatg ggatttatga
    14941 aacgattctg attgtttctt atcttatcgt cttagttatt ccatttacga tctacgcatg
    15001 ccgtcataaa ttccagcatg cagaaaaata aaaaatgaaa gctggttatt caaatggaag
    15061 taaaagaatt agaacaattg attggaaaaa atttccacta ttatcaaaca ggacaagtgg
    15121 cggattacat tccagcatta gctgaagtgg atcccaaaca attaggtatg gcgatttatg
    15181 atttggatac aaaacaactg attgagggag gagacgccga tgtgcgcttt gccatcgaaa
    15241 gcatgtcaaa agttcccgta ttgttattag caatcaaaga caacggaata gaaaaagtct
    15301 ttcaaacaat caacacggaa ccaaccggtt ttgccttcaa ttctatcatg aatatggaaa
    15361 tcaatcatcg caaacatcca atgaatcctt ttgttaatgc aggagcgatt gcaacaacct
    15421 cgttgattgc cggtaaagat gcagatgaaa aatttgagcg aatcttgtca tttatgaaag
    15481 agatctgtga tgatcctaat atcacattga atgaagaaat ctatcattct gaatcaagaa
    15541 caggtgatat caatcgctct cttgcttact atatgaaagg taatcaaatg atcgaaggag
    15601 acgtaccgga gatactcgat gcttatttca gacaatgctc cctcaatgtc accgctacag
    15661 gcatagcaaa attagctgct gtgctggcaa ataaaggaat tgctccttgg aatggcaaac
    15721 aaatcattac tgaagaaagt gctacaatcg tcaaatcaat tatgacaact gctggtttgt
    15781 atgatgaatc aggagagttt tccgtccatg ttggtgtacc tgcaaaaagc ggtgtaggcg
    15841 gcgggttgat ggcagctgtg ccaaatcgtt acggcatagg tgtcttcagc ccggcactag
    15901 atccatttgg taacagcgca gctggtatcc aattgttgaa agatgtagtc aaagaactag
    15961 acgctgacat ctttgaataa ccattcttga aagaggctgg ggtaaaagtc caaacggctc
    16021 aaagctacct acaatgaggc tgaaattgtg agcttgtttg acttttgtcc cagtttcttt
    16081 ttttattggt ttttgaaagg gcttttctaa ataaaaagga aacaaaaata cgatttacag
    16141 atatgattaa ataaaatcat aagaaaaact ggaggcgtgt atcaggaatg ctggaaaatt
    16201 acatcgagcg aaatatattt agaaaagtgt atctgtgtga acaattattt gaattccaag
    16261 aaatagacat cgaacaaaca gctatttctc taagggtgac tacaccaacg atccttcacg
    16321 atctagaaag tctagccgag tgtctagaat attgtataaa agaacaagta agagagaagc
    16381 ataagtataa actcgtattc aaacatggta ttgctctttc tgagctcaca cagtttctgt
    16441 acggtcaatc ttatttttta aagtttttaa gttattattt taatggtata ttcacgagta
    16501 cagaactggc tgatcttgaa tttatttctt tgagtaaagt atatacaatc aaaaaaatcg
    16561 tattggattt ttttaaagaa aatgattatt tgaaaaataa agaaatcatt atcccagaat
    16621 ttgactcgcg gaacatcttg ctagctttag tacgttatgt caattgggaa ggctatgaaa
    16681 ataaaaatcc gtatgtagaa gaagcttgta acgagttgat tgattatgta gagactcatt
    16741 tttttaaaag aagatattcg gaagatgaaa agtcctttat catacgagaa atagagatcg
    16801 caatcggaag aaaggcattt ccccttggac tgaaagaaga agataagaaa agcgcagaaa
    16861 cgaagccgtt atttcaatta gtctgtcaag gattggaaga gcaaaaagga attctagact
    16921 tacaagaaga agatgtctat tatatttttt ctttgttcaa cagtagaaat tataccaatg
    16981 agaacatgga gctgttaaga aaagatttcg aagttgttta taaaaatttt gttctcaatg
    17041 acctatcact gaatgaattg attttacaga ttattagtat cgctgaaatc gacgatgcag
    17101 aaaattttat tttcaagcaa tcctttttac aatttgtccg tacgttgtgg gcagatggac
    17161 aagtattttt accggaaagg atctatcttc tatcagaaaa agaacaactt ctgtacggaa
    17221 aaattgtaga aatattggaa gagtggaaaa agaaaaatca aatccaacta agatggaatg
    17281 aaaatttcat tcgaaaattc gtcaaaagtc tttctttgat caacagtgaa caagcgggga
    17341 gacaggaaat agaaattttt gttgtttcag aaagttcagt caaacaattt ttttatcgaa
    17401 cccaatttca tttgtatgcg gaagaaggca tcgctgaaat aaatcctatt ttatttcgtt
    17461 cattagaaga attgcctgat gaatgtctat gtgcgaaaaa aagagtggtt ttgtgcgatg
    17521 ctgctagcta tcaaaatgga ttagaaactg aaaagacaaa gatctatcca atctcattaa
    17581 aaggtgcaaa catccatttg tatcatattg ctcaggaact tcacctatta aaaaaacata
    17641 tttcttgatg agaccttaat taggagacct tctacaaagc tttttaattg tctgtagaag
    17701 gtttttattt gagtcctgat tcgtgcgaag atctacttat ataacaagct ttctctgaaa
    17761 tttttttaaa atgctattga cttagagtca actctaagta gtagaataga taaagtgtaa
    17821 caaatctatt ttcattaaaa gaagatagta cggaggagag aatgtattat gagtacagta
    17881 ttacatgtat tggatgcaat caatagcccc gcagatgtca aaaaattatc tgttgaggaa
    17941 atgaaacagt tggcagaaga gatgcgccaa aaaatcttgc aaaaggacag cgatgtaggt
    18001 gggcacgttg gtccgaatct aggtgtagta gaattaacga ttgcgtttca ttatgtattt
    18061 gattcaccaa aggataaaat cgtttgggat gtttcccatc agtcttatgg tcataagctt
    18121 ttaactggtc gaaaaaatgg ttttgaaaaa ggacattacc gtgaagtgag cggatattcc
    18181 aaccaacatg aaagtaaaca tgactatttt acagtggggc acacatctac atctatcagc
    18241 ttagcaacag gtttagctaa agcgagagat ctgaagaaag aatcaggaaa tgtggtagca
    18301 tttatcggtg acggctccct aagtggtggc ttagcttttg aaggattgaa caatggtggt
    18361 gttttacaat ccaatctaat cgtattagta aacgataatg agatgtctat tgatgagaat
    18421 catggcagtt tatataaaaa cttagctgag cttcgtcaaa ccaacggtaa aagcagcaat
    18481 aacctattta aagcaatggg atttgattat cgttacgtgg ataatggaaa cgatctggaa
    18541 acggtgatcc gtttatttga agaagtaaaa gatattgatc atccgattgt acttcacgtc
    18601 catacggaaa aaggtcgcgg ttatcagccg gcaatcgata ataaaatgaa atatcattgg
    18661 catgtaccat ttgatctgtc caccggggag gcaaagcaag taacttctgg agaaacattt
    18721 aatatgatca tcatggatta tttggataag aaaatgcaaa caagtacagc tcctattgcc
    18781 gcagtcaatg cagcgatacc aggcgccttt ggactgaaaa cattccaaga aaaacatcca
    18841 gatcattatt ttgacgtggg gatcgctgaa cagcactcta tcactttcgc ttctgctttg
    18901 gcttctcagg gagtacgtcc agtcgtcttc caatcaagta ctttcttgca acgggcatat
    18961 gatcaattat cacatgattt agcgatcaac gagaatccag tgattatttt agtcggtggc
    19021 ggaacgatct cagctagtga ccgaacacac caaggaacat ttgacattcc ttacttatca
    19081 agtatcccga acatcaaata tcttgctcca gcaacaaaag aggatatgat ctctatgcta
    19141 gattgggcgt tcgaacaaac agcgcagcct gtagcaatcc gaattcctac acatggtgta
    19201 gaacatggta cagctatcgc aaatgattat agtattccag cctatcaaac agtaaataat
    19261 ggaggaaaag tagcaatttt agctttaggc ggttttcttg aattaggaaa agaaacagta
    19321 aaagctttta gaaaaaaagg aatcgaagca acgttgatca atccaggaac gatcacagaa
    19381 aatgacgtag aaacattaga taaattaaaa gaaacacacg aattagtcat cacattagaa
    19441 gatggtagtt tgtcaggagg atttggcgaa aatatcagtc gttattatgg tgattcttca
    19501 atgaacgtat taaattttgg tgcagagaaa gaattcacgg atagcgttcc tgtagggcag
    19561 ctttatgaac gttatcattt aacaccaaaa caaatcgttg aagatagtct gcgtgtcttg
    19621 aatattcaat aattcattat gctcaccatc cagataattg tttatactag tatctggatg
    19681 gttttttcat tataaaaagc aaaggggtgg atgttatgta tcagcaagta gaagaatatg
    19741 tcaaaaagtt ccaagaaaaa tatcctagtt atcaatcaga aaaattaggt ttcttttcat
    19801 ttggggatac agaaaaaatg gcagacgagt tgagcgaact agttgtaaaa aagataaaga
    19861 ctggtacatc aagtggttac gatctgtata aacaagagaa tgagccttta ccaaaagttg
    19921 gtcaattaga cgtggtcctg aatggaaaaa atgaaccagt ttgtatcatc aaaaatataa
    19981 atgtgaccat cttgcctttt agtgaagtca cgaaagagca tgcttggaaa gaaggagaag
    20041 gggatcggac attagcttat tggcggaaag tccatgctga gttttttaaa catgcttata
    20101 aagaagcgtt gaacatagaa tttacagaag agaagctggt cgtatacgaa gaatttgaag
    20161 taattttctt ttagtaagaa aggtctgctg aaagaaatag ctcattattg tctcctgaat
    20221 agaaaagggt tacattttta tatgaagctg tatttttata taacattact ggaggagata
    20281 ttgtggatac actggaacaa atcaaaggcg gagtcgtcgt ttcttgtcaa gctttggaaa
    20341 acgagccgct acacagttct tttattatgg ggagaatggc tttggctgca aaagaaggcg
    20401 gagcagttgg tattcgagca aatacgacag aagatatcaa tgaaatcaag cgaatcgttg
    20461 atttaccggt aatagggatt atcaaaagag attacgatgg gtcagaagtg tttattactg
    20521 cgacagaaaa agaagtggaa gaacttttgg agacgcaagc agaaatcatt gcactggatg
    20581 caacttctcg taaacgtccg acagaaaaaa caactgctga acttgtccaa atgattcacg
    20641 aacacggtcg gttagctatg gcagatattt cgacttttga ggaagcgctt caagcgcaag
    20701 aagatggttt tgatcttttg tctacgactt tagcaggcta tacagaatat tcacgaaaga
    20761 cgcagacacc tgattttgat ttattgaatc aaatcataga acaagtcgat atcccagtaa
    20821 ttatggaagg acatactgac tctcctgaac aagtagagaa agcatatgaa ttaggtgctt
    20881 tttcagtcgt agttggatct attatcaccc gtccgcaatt gatcactaaa agatatgtcg
    20941 aagccgcaag taaagcaaat aactaaaaaa cacgcacggc acagattctt gacaaaaaag
    21001 aaaaattgat agatagttta aagcagattc taagaagatg aaaaaagcat tacttttcat
    21061 tttgtcttca agatctgctt tttaacgaat tttatagaaa gaaggcgaaa ccatgtttga
    21121 acggagtcct tcagaattga cagaacgtga aaattacaag ttgctgattg gttcagtcat
    21181 ccctagacca gtagcagtag taacgactaa atcagcaaaa gatatagtga atatcgcacc
    21241 cttcagttac ttcaatatcg tcagttcgaa tcgacccatc ttatccttgg cgatccagag
    21301 aaaacaagat gcagtaaaag acacagcaaa gaatattctt gagacaagag aagctgttat
    21361 ccatatcctt gatgaggaca acgtagaaga agtaaataaa acagcagcca atctgcctaa
    21421 tgaggaaagc gaactgtacc tgactgactt gactttaact gattcagtaa tcatttcagt
    21481 accagcattg acagaagcaa aagttcgttt tgaaacatcc ttatatgaac atgtcgagat
    21541 acggaataaa aataaagtaa cagctgattt atttttgcta gaagtacaga aatatcattt
    21601 aaatgaaaca gtctatgatg ataagacagg aaggatcgat ccaagaaaat tgaatgctgt
    21661 aagccgtttg gcaggaaatg attatgcagc aataggagaa attttcacga ttgcacgtcc
    21721 cgaataaaaa ggagcactag ttatgttaaa aataagctat aaaccatcga cagattcaaa
    21781 agaaatgaaa aaagaatatg aaacagtgaa tgattttctg caagggcagt acttagaagt
    21841 tccgccattg caagaccatt ttgtcgttac tactgttact ttagatggta aggaaataga
    21901 aatgccagat caaacgatta gcggattgtt taattacttt aacaaataaa tatttattcc
    21961 gaaaagtgaa aaaacatata aaaaaacaca taaaactaaa aaattatcaa agaatcctgg
    22021 tgcaactgaa agaatttgca acgggattct ttcaacttat ttgttatttt tttctaaaaa
    22081 atattaaaga ttgttaaatt tcactatttc atttcaaatt ccattgccta atcattagga
    22141 aaagtctatc atgaactcgt aaatagaata tagtaataac cttgttaggt gaggctccta
    22201 tacaaacata ggctgttgcc cagaaacgtc gaaagtcgcc aatgggtaaa acagaaactg
    22261 ttggattaag gctttttcca atagctaaga atgttttctt tacgttgtat agtgctaaaa
    22321 ctcggacgat gagatgatgc taggtcagaa ttcttgttcg aaaaggtttc tgaccttttc
    22381 tttatatttt tgtgactgac ttatttgatg agacaaactc gctcctcacc tgacaagtgg
    22441 attgctgtgt gaggaggaga tgcggtgaaa aaagatcgat tggtggattt tgggtcctta
    22501 tttgactttg tccatgatcg gtcttttaga agtatacagt gccagttctt atcgtttatt
    22561 gcaagccgat gaaaatacaa aaagtctatt attgcgtcaa ctaattttca tatttttgag
    22621 ttggagcgtg atcttcttag ctcgttcagt caaactacac tatttacttc accctaaaat
    22681 agcaggatac ggtttagcct tatcgatttt ctttttagta ttagtaagaa tagggatatt
    22741 cggtgtcact gtcaacggcg cacaacgttg gatctctctg tttggcattc aattccagcc
    22801 ttctgaactg gcaaatcttt ttttgatttt ttatttaagc tggttttttc gtgacggaaa
    22861 tagtagccca aaagatctaa aaaaaccatt cctgattaca gtaggtataa cttttctgat
    22921 tttatttcag ccaaagattg ctggagcatt gatgatcctt tcgattgcgt gggtcatatt
    22981 ttgggcagcg gcggttccat ttaaaaaagg gatctatcta atcgttactt tttctgcatt
    23041 gctgattgga gcagcaggcg gggtattata tttaggaaat aaaggttggc ttccacagat
    23101 gtttaatcat gcttatgaaa ggatcgcgac tttaagagat tcttttatcg atagtcatgg
    23161 tgcgggctat caaatgaccc attcgtttta tgcactatat aatggtggca tatttgggcg
    23221 gggattagga aatagtatca caaaaaaagg gtatcttcca gagacagaaa cagattttat
    23281 tttttcgatt attacagaag agcttgggct aatcggtgca ttgtgtgtgc ttttcttatt
    23341 gttctcatta tgcatgagaa tcttttgttt aagcagtcgc tgtaaaaatc agcaagctgg
    23401 attgtttctt ttaggattcg gaactttgct attcgttcag acgataatga acgtgggaag
    23461 tatcgcgggg ctgatgccaa tgactggtgt gccattgcca tttgttagct acggaggaac
    23521 aagttatctg atcctttctt taggaatcgg aatcacgttg aatatttctt caaagataca
    23581 ggccgaagag ttggcgctct atcgacctga aaaacaataa agagaaactg agaaactctc
    23641 agtttgaatt atcataaagt acaatctttt atttttcaag cgagaggttc ttttagaaag
    23701 aggaattcgc aatgaaagta tctcaaaaaa tcatattatc tattttgtta ctgcttacat
    23761 tagtagttgg ctatttttcg attgagttta tccatggatt ttcgtctgca aaacagagtt
    23821 caactgtgaa aaaggtagag ccgaaaagta tccctaccac actaaatgtg gctttgattg
    23881 gttcagatgc tcgttcgaaa gacgaaaacg gtcgctcgga ttcacttctg gttgcacaat
    23941 acaaccagaa aacacaacaa gcaaaactga tctctatcat gagagattca tatgtcgata
    24001 ttccaggtta cggaatgaat aagataaatg cagcttactc ttatggggga gttgatttat
    24061 taaatcaaac attaaaggaa aatttcaaat ttgaagctcc ctattatgca agtatcacat
    24121 ttcaagattt tatcgattgc gttaatgaac tgtttcctgg tggagtcaag atcgatgcag
    24181 aaaaatctct agatttagat ggcgtatata taaacaaagg agagcaagtg atggatggta
    24241 atactttact ccagtatgct cgatttcggg aagacaaaga aggagacttc gggaggatca
    24301 gaaggcagca gcaagtgatc aaagctatct ctcagcagct gaaagacgta acttcaatat
    24361 tcaagctacc caaagcggtt gggaaattac ttggtagtat ccaaacgaac cttccagaaa
    24421 gtgtgcttct ggattgtggt atggatttcc tcaaagacat atgttagaat aaacaggtat
    24481 aaatagtgaa ataaaggaat gacaagcaag agaaggagga aaaaatgaaa agaagtgaca
    24541 agcacggcaa aaatcgaaca ggcgcttata ttgccggcgc agtgatctta atagcagctg
    24601 cgggtggcgg ttatttttac taccagcact atcaagaaac ccaagcagta gaagctggag
    24661 aaaagacagt tgagcaattt gtccaagctt tgaacaaggg agattataac aaagctgcag
    24721 aaatgacatc aaaaaaggcg gcaaataaaa gtgcattatc tgaaaaagag atcttagata
    24781 aataccagaa tatatacggt gctgctgatg tcaaaggact tcagatatcg aatctaaaag
    24841 tagataaaaa agacgattct acttacagtt tttcatataa agcaaagatg aatacctcat
    24901 taggtgaatt gaaagatctt tcttataaag gaacattaga cagaaatgat ggtcaaacca
    24961 cgatcaactg gcaacccaac ttggtttttc cagaaatgga aggaaatgac aaagtaagcc
    25021 tgaccacaca agaagcagca agagggaata ttatagatcg aaatggtgaa ccattagcaa
    25081 caacaggcaa actaaaacaa ttaggagtcg ttccaagtaa acttggagat ggaggcgaaa
    25141 aaacagccaa tatcaaagcg attgcttcct ccttcgactt aacagaagat gctatcaatc
    25201 aggcgatttc acaaagctgg gtacaacccg attactttgt cccattgaaa atcattgatg
    25261 gagcaacgcc agaacttcca gctggagcta ccattcaaga agtagacggc agatattatc
    25321 ctttgggtga agcagctgct cagctgattg gttacgtggg agatatcaca gcagaagata
    25381 ttgataagaa tccagaatta agcagtaatg gaaaaatcgg tcgctcaggt ttggagatgg
    25441 cttttgataa ggatcttcgt gggacaacag gtgggaaatt aagcattaca gatgcagacg
    25501 gtgtagagaa aaaagttctg atcgaacatg aagttcaaaa tggcaaagat atcaaattga
    25561 caatcgatgc gaaggcacaa aaaacagctt ttgacagtct aggaggaaaa gctggatcaa
    25621 ctgttgcgac aacgccaaaa accggtgatc ttcttgcgct tgctagctct ccaagctatg
    25681 atccaaacaa aatgacaaac gggatctcac aagaagatta caaagcttat gaagaaaatc
    25741 ctgaacatcc attcatcagc cgatttgcga caggttatgc tcctggatct acgtttaaaa
    25801 tgattacagc agcaatcggt ctcgacaacg gcactatcga tccaaatgaa gtgttgacga
    25861 tcaacgggct taaatggcaa aaagacagtt cttggggatc ttatcaagca acgcgtgtca
    25921 gtagtgatgt atcacaagta gacttaaaaa ctgctttgat ctattccgat aatatatatg
    25981 cggcacaaga aacgttgaaa atgggtgaga aaaaatttcg tacaggctta gataaattca
    26041 tttttggtga agaccttgat ttgccaatta gtatgaatcc agcacaaatt tctaatgaag
    26101 atagctttaa ctcagatatc ttgctagctg atactggata tggacagggc gaacttctaa
    26161 ttactcctat ccagcaagca gcaatgtatt ctgtttttac caacaatggc acacttgtct
    26221 atcctaaatt gattgcagat aaagagacaa aagataagaa gaatgtcatc agcgaaacag
    26281 cagtacaaac gatcgtgcca gatctgagag aagttgtgca agatgtaaat ggtacagcac
    26341 attctctttc tgctttaggg attccgttgg cagcgaaaac tggtacagcg gaaatcaaag
    26401 aaaaacagga tgtaaagggg caagagaaca gtttcttgtt tgctttcaat cttgataacc
    26461 aaggttatat gatggtcagc atgttggaaa ataaagaaga tgatgattca gcaaccaaac
    26521 gagcatcgga actattgcaa tacctcaacc aaaattatca ataaaaaatt gctttttaag
    26581 tacagtaaaa ggactgtggc gttatcgtca cagtcctttt ttcaaataaa cggttttcac
    26641 gatgtctctt tcacaacttc tttttgattt actcacactt cataaacttt ttttgtaaaa
    26701 tatttgagaa caagtggaga aataagtgta cttaataaaa tcacgataac taaaggtgaa
    26761 tagtactgtg gttcgatcaa ttgactttgc tgtccaattt gtaaaatgat caaagccatt
    26821 tctccacgcg aaatcatacc agcaccgacc atcaatgaac tatgttgaga aaaaccggca
    26881 atctgagcac caaaataacc tccaagcaat ttggtcaaaa tcgctacaag cgtcaatata
    26941 gaaataaaga gtagttgttc actaaagtca gaaaaatcaa cttctaatcc tacactgaca
    27001 aaaaatactg gtataaatac tgcatatcct aaagcctcta cattgtggta tacttcttct
    27061 ttaacctttg tctgactgac agctatccca gcaaaaaaag caccaatgac agagctaagc
    27121 ccaacaagat ccgctacgta agacattcct aaacaaataa ctagcgatac gatgataata
    27181 gaagaatttg cgtacacttt ttctgcaaat gaaagtagga aaggcgcaat ccatttgact
    27241 aataagaaaa taaaaacaaa ataaattagc tgttcaatca aaacaagcgg taaagaagca
    27301 ttgctagaag cttcccccgt tagaaaagat aggctgaaac ttagaactaa aacgactaaa
    27361 atgtcatcta ccacagaagc acccaaaatt gttgatcctt cttttgtatt cactacgttt
    27421 agttctttta atacttctac tgaaatactg acagaagttg ccgcaagaat aatcccaaag
    27481 aaaatcgctt catttcccga aacttggaat gctttgcctg tcaaccagcc aaaaacaacc
    27541 gggaagatga tcccaagcaa agcaacgaac ataccaggtc ggaaatattt tttcaaaaga
    27601 gataaatcac tttccagacc agctaaaaac attaataaga tcacaccaat ctccgaaaag
    27661 tcatggacta gaatatcagg tttgacccaa ttcaatccag cttggcctag caaaacacca
    27721 acaagtaatt gtccaatcac tgctgggata cctaatctcc gcgagatatg tgagccaatc
    27781 gttgttgaaa tcaatatcag gcataaaata ccaataaatt ccattctcat cattcctttg
    27841 tataaaatat agcccgaact tagcataaca tacattattg aaaaaatagg aaaatatact
    27901 cacaaaaaaa ccaacaatcc aaaagatttt tttggattgt tggtttttat acggtctatt
    27961 ttaataacta aaaatgatta acgtatagtc atttttctta ttctgatgaa attgataatc
    28021 tcctggatgc ccatgaaaat caaaataaag ccgaatattt ggaatagaag catcaaactt
    28081 ttgaatggat tgaacaataa gacgaatccg ccaatcaaca acagaatact actataaaca
    28141 ctccaacttt tagcttttgt attcatgctg aagaacaact ggaacaagct gttgatgata
    28201 acagctaatc caagaagaat cggcaggata gaaacgattg caggtgcaaa agcatagacg
    28261 atcagagcta agatcaggaa aaaaagacca ctgaacagac cgatactcca agaacctgtt
    28321 ttccttttaa aacggtaagc ctcaaaaaga ttcaacaacc caagcaaaac aagataggcg
    28381 gtgatcagat aaccgataaa atgaaaaaca gcagaagggt ttagcacaat cgcaatccca
    28441 gaaataatat aaacagcagc tcttaataag gcgtattttt gaatattttg tttaatcgtc
    28501 tcaaacatat aagaaaacct cctttgactt attttaacgt gaactagtca ttttaccaaa
    28561 ttaaatgtta caaaatgagc gaaggagaag aatatctctg gaaaaatcaa cttctgagga
    28621 tcttttccat ttttttgcct tttgctagct catcgatcat tttatctaaa taacgaattt
    28681 ctttcatcaa gttatcctcg atgtcttcta ctcgtacacc acaaacaaca cctgtaatca
    28741 aggaacgtga aggattgggg acaggggctt ccgcaaaaaa ttgttcgaaa gtcacttgtt
    28801 tttgtagttg atattctagt tctgtttggg aataacccgt cagccaacaa atgatttcat
    28861 ctacttcctc ttttatacga tcttttctct ccgcttttgc tacatacata ggataaacgc
    28921 ttgcaaaact tgtcgtaaaa atacgatttt gcgccataat cagtcacctt tctttttttc
    28981 aaattattta ctacttatag tatataagaa aaagagaaga aaaagcgatt attcaaaaaa
    29041 acaaaaagga ttgtgacaaa attcttgtca ccatcctttt ttccgctctc tgacttttta
    29101 caatcttcat agctcaagtg cgcatcagat aatcgaatgc tcctaaagct gcagtagctc
    29161 ctgaacccat tgaaacaatg atttgtttgt atggactatc tgtacaatca ccagctgcga
    29221 aaacgccggg gatattcgtt gcgccatttt tctcggtcac tatttcattt cttggattca
    29281 tttcgatcgt tccatctagc caatccgtat tagggagtaa gccgactaga atgaatactc
    29341 ctgaaacagc tagtgtatgc atctccatcg tacggcgatc gatataggag agagattcta
    29401 cctgattcgt accgctgatt tctttcgtcg ctgcatgttt gataacagtg acattcgcaa
    29461 gagagactag tttatcctgt aagatttgat ctgcttttaa ttctggtaag aattctagaa
    29521 catatacatg ttttgctaac ccagccaaat cgattgctgc ttcaacacca gaattaccac
    29581 caccgataac agcaatctct tttcctttaa ataaagggcc gtcacagtgt gggcaataag
    29641 caattccttt atttttaaac tctttttcgc ctggtacatt gatagaacgc cagtgcgcac
    29701 ctgtcgcgat gatggcggtt tttgctttaa gtgatatacc gttagctaag ccaatattaa
    29761 taaaatgttg ctctttattt aaggaaacgg cctgttgatt catcataatg tcgatagggt
    29821 aactacgcac atgactttct acttgctgca tcaattttgg accttctgtg taagaggtac
    29881 caatcatatt ttcaatccct agtgtttcaa cgacttgtcc gccgaaagaa tcagcgacaa
    29941 gtcctgtacg gattccttta cgtgctgcat agatagctgc actactagct gctggtccac
    30001 cgccaatcac taatacatca taaggttctt tttcaaacaa ctcttcttca atcaaagggc
    30061 cagtgatttt ttcaacaatt tgttcgatcg tcatgcgtcc gctactaaat tcttctccat
    30121 ttaaaaacac agttggcact gccatgattt ttttatcatc gatttctttt tggaacatcc
    30181 caccttcaat catcgtatga gtaatatttg gatttaatac agctaatata ttcaatgcct
    30241 gtacaacgtc ggggcaatta tgacaagtca agctaacata actgtcaaaa tgcaatgttt
    30301 gtgttatttt tttgatttta agactcaaag attcactgat tttcggtgcc cgtccactaa
    30361 tttgtaacat agccagtaaa aaagaggaaa attcatgacc gagggggata ccagcaaaaa
    30421 cgattccact ttctttgcca actggattga tagaaaaact aggtgtgcgc atcgaatctc
    30481 ttttttccca agagatttta ggagacattt ctgctatttc cttcgtaaat tcagctagtt
    30541 tttgactatt ttcactatta tccaaactaa cagaaaaaac aataggagat tcaatcagtg
    30601 ctaaatattg ttcgagattt tgtttagttt ctttgtctaa catatccacc acctaaatct
    30661 ttccaacaag atcaaaactt ggtttcaatg tttcgccact ttctttccat tttgccggac
    30721 aaacttcacc tgggtgggta cgtacatatt gagcagcgcg aatcttatca atcagtgcac
    30781 tagcgtcacg accgattcca tctgcattga tttccatagc ttgaacgatt ccgtctggat
    30841 caataataaa tgttcctcgc tgagctaatc cttgctcttc atccaaaaca tcaaaaatac
    30901 gagaaatctg atgagacgga tcaccgatca tcacgtattc gattttaccg atagcatctg
    30961 atgtatcatg ccatgctttg tgagtaaagt gggtatcggt agagcaagaa aagacttcaa
    31021 cacctaaaga tttcaatgta ctgtattgtt cttgtaagtc ttctaattca gtaggacaaa
    31081 caaaagtaaa atcagctgga tagaagcaaa gaatactcca gttccccata agatcttttt
    31141 cactgacttc taaaaattct ccttgatgat aagcatgtgc attaaattct tcgatttttt
    31201 tcccaatcaa tgacatagta aaacctccaa taataattat ttatttataa ttattgtaat
    31261 ttagttggag ttgcaaagtc aatggatatt ttgttattta ataaagatta ctcaaaaacg
    31321 aaggttaatt ataaaaatag aagaggtcgc aagaaaatgc tactgctgga aatactcggg
    31381 ataacgttga gtaacagttt cctgttcttt cagcatcatg tgtaagtgca atcccgtttg
    31441 aaatcgagct tcttctggtt ttttatcaac taaagccttg agtaaatcat aatgttgttc
    31501 catgatcgtt tcccatggaa gcccctgaat ggtcactcgt aaccagcgga aacgttctaa
    31561 atgggtatta tggtcatcta accagttcca aacttctttt cttcctgcaa tttcgaaaca
    31621 agcttcatga aataaattat ctgcacggaa aaaagcgcgt tcgtcattta ttttgactgc
    31681 tttttcttgt tcttctaaaa tcgtttctag cactattcgt gtttgtgtgt ccaatttgga
    31741 acaacattca atcatgattt gctcttccaa ctgttctcgg acaaagcgag cattttctgc
    31801 agaaacaaga ttaattttgg aaacatacgt accactttga ggtatcgtat acacgagttc
    31861 ttgctgacgt agttgaatca atgcttcgcg aatgggagtc cggccgatag caagatgttc
    31921 ctccagtgcc ttttcagata ttttccttcc aggttctaag tcagcataga taatttgttt
    31981 acgaatcgct tgataagctt gtgattgcaa gctgcttttc atctttattc ctccaaaatc
    32041 atttctatga acgtagtata acagaagagg tctgaaaaac aatctggtat gtcacattaa
    32101 ctagtaaatt tgtataaata gtaaaatttt ggttgacata gcgattttta ttgcataaaa
    32161 ttcacttgaa tacaaaaact aatatgttag tttaggaggg gaaaaatgat tgatttaaac
    32221 aactggagga actttatccc tgaattatct caaaaagaag tagaaatacc tcattttgac
    32281 cgtgcatcat taagagaaaa tggacaaaaa aatcctgtct ggatccattt cggtggcgga
    32341 aatctttatc gaggatttca tgcagaaatc gctcaacgtt tagcggatct aggcgaattg
    32401 tccactggtg tcattgtttg tgaaacattc gatgaagaaa taattgaaag ggcttaccaa
    32461 gattttgatg atttgattct tgaagtggtc atgccaagca gtggagagtt cgtcaaacgt
    32521 atattagctg cgacagctga cagtctgtat tgtcatccag attgttctga acactttgat
    32581 aagatgattc gacactttga aaatcctagt ttacaattag ttacttttgc tattactgaa
    32641 aaaggttatc gattgaaaga tacaaataat aaatatatag gaccgattgc agtcgatata
    32701 aaaaatggtc caaagaagcc atcacataca atgagtattc ttgctgtgct actttataaa
    32761 cgttttttat ctggagcttg gccgattgca cttgtctcta cagataattt ttcacaaaac
    32821 ggtagtcaat tacgtacttc tttgctaacg atcgcaaaag aatggcaaaa ggaaggttat
    32881 gtacaaaata catttatcca atacttatcg aatgaagaaa ttgttgcgtt tccatggacg
    32941 atgattgatc gtatcacccc gaatccttcc ttgtcagtta gcaaaatgtt ggcaaaagag
    33001 ggcttttcaa atatgtcgat tttccaaacc gcaaaaggaa ataatattgc tccatttgtc
    33061 aatacagagg acatccatta tttaatcatg gaagatcatt ttcccaatgg acgtccgaaa
    33121 ttcgaaaaag ctggggtatt cttgactgat cgtgcaacgg tagataaagc agatacgatg
    33181 aaggtgacga cttgccttaa tcctttgcat acgtctttag ctattatggg ttgtttactg
    33241 gggtatagga gcatagctga ggagatgaaa gatccagatt tatcgctgct agtttctgaa
    33301 atcgggtaca atgaaggcct cccagttgtg gagtctccag gtatcattga tccaaagatc
    33361 tttctggaag aagtaattac tgtgcgcttt tctaatccaa atattcctaa tatgcctcag
    33421 cgaatcgcca cggatacctc tcaaaaggtg gcgattcgtt atggtgagac gattaaaaaa
    33481 tatatggaaa tagaagttaa agacccgcag acacttgtat tcataccatt gactctcgct
    33541 gcttggcttc gataccttat tggcattgat gatcaaggaa aaccattcat cccgagtcca
    33601 gacccactat tgacagacct tcagcataag ctttccgata ttcaactggg tacgcaagat
    33661 acatcacata ttcatgagaa aatggctcct attttgtcaa ataaagaaat ttttggtgta
    33721 gatctttacc aagcagagct tggcagaaaa atagaagaaa tgttcatcaa gatggtagca
    33781 ggaccaaatg ccgtacgaaa aacattacag gattacttac aagaacatgg aggaaattta
    33841 tcatggaaat gacttttaga tggtacggat cacaggaaga aaagattacc ttagatgaaa
    33901 tcagacagat tcctgcggtc aaagggattg taggcacatt gatggatata ccagtaggag
    33961 aggtatggcc tagggagagg atcaaagaac taaaagaaga aattgagtct cgtgggttaa
    34021 cactaaaagt aattgaaagc gtcaatgtgc atgatgccat taaaattgga ttaccttccc
    34081 gtgagcagta tatcgaaaac tataagcaga caatccgaaa cttggcagaa tatggaatag
    34141 aagtcatctg ctataatttt atgcctgtat ttgactgggt gaaatctgac cttgactacc
    34201 gcttagaaga cggctcatcc acgttagctt ttatatctga tgatattcca gatgatccaa
    34261 gagaaatcgt cgcgcgcatc gaacattcca gcaatgaatt tggattgccg ggatgggaac
    34321 ctgagcgctt agctcctatc aaatccttgc ttgaagccta tgcatcagta gacgaagaaa
    34381 aacttcgaga aaattttgcc tattttctcc aatcaatcat cccaacatgt gaagaggtag
    34441 gggtcaaaat ggctgtacat ccagatgatc cgccatactc gatttttgga ttagctcgaa
    34501 tcgtaaagaa tcgagaagat ttagattggt tgtgtcgcat agtagacagc ccttataacg
    34561 gaatcactct ttgtacaggg agtattgcag aagatccaga aaatgatgta tatgggattt
    34621 tagcagaatt ttgccaaaga gatcgaatcc catttgcaca tgttcgaaat attaaatttt
    34681 tagctaatcg agatttctat gaggcaccac ataaatccga atacggttca ttagacatgt
    34741 accgcatact aaaaacaatg tatgacaacg gatttaacgg atacatccgt cctgatcatg
    34801 gaagaatgat ctggaatgag acaggaagac caggatacgg cttatatgat cgagcattag
    34861 gtgctgttta tctaaatgga ttatgggaag caatcgaaaa aggaaacaaa agtaaagata
    34921 attagtgaaa aaaactgtaa ttttattatg taaataagaa ggatgttcaa attcttgata
    34981 cataaatccg gaggttgtta ataagcattc ggatttatgt atcttatttg gtctaggaag
    35041 taacttgatt ttcttttttc gattgatcca tgagtgcttc ttcttttaat tcaaagatat
    35101 attctgagat cctcataatc acttttttcc agtcttctgg tgtgatgatt ccttttaaaa
    35161 tatagatttt tttgatatga gaaggcaatt gtttcaaagg gcagtcgctg ataacgatgt
    35221 tgcagttctc cataaaatca ttttgtattt tgatcgattc atttgaaaaa atttgttcta
    35281 tcttaacgat cagatcttta ctgatataaa aatcacgcgt atagttcaca ccaatcacaa
    35341 ttcgcggctg ttttttgtag cgatcataca aatgtactag gtcttcaacc atccattgca
    35401 cattttcctt ttctaaaata actggtaaca agtgttgatt ttgttctctg aaatccatat
    35461 aaaattgttc aatggcggtt tggacttctt tcaatacgct atgttcttga taaagcatga
    35521 aagtaggaag gggcgtattt ggttgattga agttaagaaa tccattataa agtttatttc
    35581 gtgcgaatgc caatgagtaa agaatctttt ctttttcagg tataaacaaa tcaaatacag
    35641 tacaaaggga ctccaccatt tctcttgaat aaagaacatg aggaacgttt gcttttttta
    35701 aaagtttgta ttgctccaag cttgtttctt ccgattcgat tttcgtgata gaaagtcgag
    35761 ctaaaatatt cactaagatg cgttgttcgc gacaaattgg cagattaggt cgcaaaatat
    35821 ctacagagag atcaataaaa aagtcaatat attcatactc tgtgaattct ttttgtacgt
    35881 ctttcacaga agttgcagga aaattctctt tacatatttt taatagaaaa tataatttat
    35941 ctatcatacc gctatttagt cggtcaagta cttctttttt aaaaaaagta ttcatcataa
    36001 aagccactac ttgcggatct tcatcataaa attctgtagc agtacttgaa aagatgttcc
    36061 aataaatatc taatgctgca tattgaattt gtacctcttt tccagaaaaa gactttctgc
    36121 cattattaga aaaggagata gacaaaccgt actatgctaa aaatgcatta atctttttca
    36181 ttttcgtata caaataagag gcactatagt ttgtttgctg actgaaactg ataatcgaat
    36241 ttgtctgaca gctgaacaaa gaagtcaaga tctgataact gactgattcg ttgcagtatc
    36301 ttccaaatag acgataatag cagtaagccg gatcaattcc ttcaaaattc aactttaaaa
    36361 aaggtttttg cttatcaata tgcagttctg ttagattgag acgctcaaag tcttgttcta
    36421 attctttgat gtatagtaaa aaatttgatt cagacatatg gaaatgatct gctaaatatt
    36481 gtgtagtaat aaagaaacga tgttcagata ggaaaagaaa aatatctaat tttgtattgc
    36541 tttttttctt caagataaac gttttaatcg cttcttgagt ttgcatgaaa gaggctcctt
    36601 tttacttaaa ttgttaaaac ttgaaaatat tactgttttt ttcataaatt tagacaaaaa
    36661 aacagtaaac atataggtag aatctctact atcttatttc ttgcatacaa aaaaatcaag
    36721 gagtgagaga gttatgagat attcaaccat cttaatatca acatttgcag ccttaagctt
    36781 atcgcttact ataaatcaga cagtccacac agaggagaca gaaacagcca aatggacaga
    36841 aaccaagtgg gccaacgtca atcgaattac ctttcaggat gaattttcat ccttcaattc
    36901 aggagtcgca aaattaagaa aaggacaaac agcgaccatc aaagcaaaaa ttgactattc
    36961 gggagactta gcacctatac tacaatcagc tgttacattt gagaatgttg atcccacttt
    37021 gtcgattggc tatgacgaga acactttgac tatccaacac aagcaagcga tatttacctt
    37081 caccgttaca ttaaatcagg acttgttaaa accagcgctt tttactgtca aggtatcaga
    37141 tggactaccg aacgatcatc atttaacgcc gtatagtcag cggcagacaa ttgagcaaga
    37201 ttcagccatc gactcaggag gagatcttgt tgaagaacca acagataaac cagaaaatga
    37261 aaataagcca gaagtaccgc ctacagaaaa tccagacgga gaacagaaac cggaaataga
    37321 acctggagaa gaacccgata cagaaactca accagagcca gacaataaat caaaacctga
    37381 aataacccct ggagaaaaac cagatgtaac tccagaacca gatacggatt ctggaaacca
    37441 aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaaccaga
    37501 ggtagatcca gaagaaaaac cagatgtgac tccagaacca gatacggacg caagagacca
    37561 aggaatacca gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa aagagagtaa
    37621 aaaatcaaat ctagcaattc taaaaataaa tgaagagcag ctgaataaaa aatcacgaat
    37681 atttgatagc gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa cctttgctgt
    37741 cctgaaacga aaaataaaca gctaccaaaa tccggagagt cacaaaacaa agttatactg
    37801 tggagcggaa tcattctatt aagtatagca acaatgcttt ctgcaaaaag atttaaacaa
    37861 aacagatctc tctaacattt gaataatgtc tgtgaacgca tttttcaaat aaaaagaaat
    37921 atccagaaaa tgaccccaaa ataccagatt gaaatctgat atttggggtc gttcatactt
    37981 tatctttctt catatttatt ttaagggata tctgtttctt ttggaagatg gttcgggatc
    38041 tttttcactt tcaatacatc taaaaagaag gtaaagatcg gaatgccgac aatcaagccc
    38101 caaacgccaa agaaccgttc gctaacaagt aagaccacaa atgtataaaa aatcggcagt
    38161 tctgttttgc tggacatcaa cttcggattc agcacataag actcaattag atggacaatc
    38221 aaaatcgtaa tcaggatata aacaacatct cggatgcctc cttgggaata agctatgaaa
    38281 gatagaggaa tacaagaaat gataacacct gctactggga tcagacttaa aatgaaaatc
    38341 atgatcgcta aactcagcag ctgatgaaag ccaaatacag ccaaggcgat tgtagtgagc
    38401 aacgtattta aaagagcgac aacaaattga gcttctaata ccaaaccaaa tgtattgaca
    38461 aacttatcgg caaaatagta aatatcttga aagaaccaag aaaattcact ttttaaaaac
    38521 aatcttgaga atacaattgt ttttttcttt tcaatcataa aaaagaaact taagatgaat
    38581 gacatagcaa acgccactgc catttttcct atatcctgta aatagccaag cgcaatcgaa
    38641 gcgccatttt gaagctgttc aaaaaaatcg tttcgctcaa tataagtatg gatatattgt
    38701 aaaacaggat ctgcatcttt tggctgatgc tcgtaaaaat taataacgga ctggatcatg
    38761 tcaaatgttt gatgaataag catcgggata tactttgtga atgccagata gataaggtaa
    38821 acgatcagtg tataaatcat gatagctaaa acacctgtag ggattcggac aaatcgctga
    38881 atgatttgaa caagatgaat catcaaataa gtaaagataa atgtgagcaa aatggttgta
    38941 atcatacttc taactaagaa aagaacaaaa acgacaaata ataaaacagt gagacggcgt
    39001 attttttcat tcgccaaaaa acgctgataa acggtcaaat cgacaacctc caaataacaa
    39061 aaaataaaca cgttattcat tatatgatgg aatatgaaaa taagaaagag caaaaagaaa
    39121 aaccgctaaa tgacgttatc ttatgagttg gttttcctca taaaatcgtt catctagcag
    39181 tttcagccct ttttctggtc ttgatgaaaa ttatctagca tttttcgggt cagatgttgc
    39241 agtgtatcca attcttcctg ttcaagtgga agataagact tgatcttttg tggaatacct
    39301 tctgcttgtt tttgcaacgc agcaccttga gcagtcagcg taacatttac gattctttca
    39361 tcacccgttt cacgctgacg attaatataa tttttttctg ctaacttttt tagtaaggga
    39421 gtcagcgtac cagaatccag acacaaatca tttcctagct ctttcactga agtctttttc
    39481 ttttcccata aaactaacat cacaagatac tgggtatagg taagattcag cggcttgagg
    39541 aggggagtgt agtgtctaat gatttcttta gaggcggcat ataaaggaaa acatacttga
    39601 ttaactaaat atagatcttc catttttcca cctccgctgt tttactatag tgtatcatag
    39661 cggatttaca caaaaaagtc tattcactaa ctgctttcag agtaacttct atatttccag
    39721 ctgtagcttt tgaatacgga cacactttat gcgctgcctc taataattct tgcgtttctt
    39781 ccagagaaag accttcgata gatccttcaa tctctacacc taagacaaag ctcgtttcac
    39841 tttctgaata aagggaaact tgtgcactga cagtagaagc cccagagata tttttttgct
    39901 gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa accagctgca aacaattgtt
    39961 cagggttggt tgcattttcg acgcgtttac caggagctgc aatattcaat tcgaatgtac
    40021 gatcgggatt atgcacttct ccagagcgtc cgcctgtgtt gatcattttc gtagaataca
    40081 tttttttcat aaaattacca cctttaatta tattttatac aattgaattt tacacaatat
    40141 aattgtggaa agcaaagaaa tggattggca taagataaat aagagaaagc acatgaaaca
    40201 gacgcgcttt ctcgaatagc aaacatatac aaaaaatatc agagacaaaa aataatctca
    40261 tcataaacat tacatatatt tctcaaaaag ttgttttctt tcttcaaaaa ttacttcacg
    40321 atggacctga tgaagcatcc ataaataacc aaaaggatct ctgaacattg agttagtcac
    40381 accaaagtcc atcatttctg tcatttcttg aatctctaca cagccatttc gtaatgcttt
    40441 attatgtgtg tcctcgatat cagagacgat gatattgaac cagatcgtat ttggatgttc
    40501 agcagtagga gcagtcaaac caagagacgt gttttcatcc agcatatgga atctcgtacc
    40561 atacaaggta aaaatgactt cattattgcc tctagggtaa tctgttgcct caatcagttg
    40621 tatttcatca aagattgttt gatacagttc tagtgctttc aaactatcat ttaatacaaa
    40681 atcgatttcc gttcctttat gcataaagat ctcctctcct tttcagtcga acagttacta
    40741 gattctttta ttataccagc taaagaaaac gcattcaaaa aaatagcgga tttacttacc
    40801 aactcattcg attggatgat aagtaaatcc gcgtcccatt acttctgcta cgctgctgac
    40861 aatgacaaag gcacttggat cgatttcttc tatcaatggt ttcattcggg aaaattcatg
    40921 atcagtcaca acgatcaata aaacctcttt tgactttcga ttatacccgc cttttgcctc
    40981 tagcagggta aggccacgtc caatctcagc acttaagcga aggcggatgt cttccaaata
    41041 atgttcactc atgaccataa cagatttctt tcgattgata cctgtttcga tataagtcat
    41101 gacaattgaa gtgattgcaa tagataaaat agcataaaga aactcttcta ttccaaaaat
    41161 aaacagatta agagaaacaa cgaatgcgtc tgtaaaaaat aagccaatcg aagtattcaa
    41221 gtggaaatat tttttaaaaa tgaggggagg gatcgtcgta ccgccgctag aagagttgtt
    41281 tttatataaa atcgcaactc ctaaagcaaa aatcgcactt ccaaaaatga cagacaacaa
    41341 ccgatctgaa gtaatcattg tttctggaac tattgcaatg attaaaggaa aaaccaagct
    41401 tccgaaaagt actttaaaaa agggtttttt acctaaaaat agaagagcta gtaccaacat
    41461 aacgatattc aacactaaaa taactatagc tcggtcaaag cctaaagcag cttccagtaa
    41521 tattcctaga ccgctgacac ctccagcagc aatatgatga ggccctaaaa acatattgat
    41581 agcgactcct aatatgaaga gggcaaaaat aatcttaatg tatgtccata taaattgtct
    41641 cgtttctttc atttattcac ttccttaatt cattaataat actcttatct tattataact
    41701 tacttttcac cttaagttaa ctttaattaa taaaaaaatt aggctagtct aaattattat
    41761 gtttcattct tctagaatat acttctacgc caaaaactgt taaaaaactt ttactttaga
    41821 aaattggaga aattgatcgc ttaagataaa ttcaggacac taaatagtac aggaagtaga
    41881 ctaaaacttt taaaagtttt aagtctatct atttggcaag tttaaaaaat aaaaattcgg
    41941 tatcaaaaaa ctgttaccga taacattttt gaaagcgttg tcttttgctt ctctatcact
    42001 tatagtgatg acgtcatatt aaaacacaag gaggaaatca atggatacag ctgcaattta
    42061 tcatcgaccg gaaagtgaat atgcatttct atatacgaat acaagatttc gtattcggct
    42121 tcgtactagg aaaaatgata tcaaaaaagt atatgtgcta tgtggagatc catataccat
    42181 aacaactgaa aaatggtatc aaaaacaacg accaatgaaa aaatgtctaa gtaccaatgt
    42241 tcatgattat tgggaaattg aagtgacgag cgaaacaagt cgcttacagt atgcgttcca
    42301 tgttgttggg gaagataaca cggactgttt ttacggtgat caaggaattt ttccttatcg
    42361 agaaaatgtg attactgaac caaattttta ttttcgaata ccttattttc atcaaatcga
    42421 tcgattcact attccagaat gggtaaaaga aacagtatgg tatcaaattt ttcccgaaag
    42481 atttgcaaac ggggacaaag caaatgaccc cgaacacact ttaccttggg gaagtaaaga
    42541 tccagaccga gaagactttt tcgggggaga tttgcaaggt gtgatggatc atttggatta
    42601 cttgacggac cttggcatca atggcatcta tttttgtccc atatttaaag ccacatccaa
    42661 tcataaatac gatacgattg attattatga ggtggatccc gcttttggcg ataaaaaact
    42721 tttaaaaaac ttgattgatg aagcacataa aagagggata cgtatcatgt tcgatgctgt
    42781 attcaatcat atgggtgtcc attcaccaca gtggcaagac gttctcaaaa acggagaaaa
    42841 atcgatctat aaagactggt tccacatccg ttttttccca gtagattctt atcagatgac
    42901 tgacttacca gaaactgctg aaaatatccc atatgatact tttgctttta ctccattcat
    42961 gccgaaacta aatacagcaa atccagaagt gcaaaaatat ttgctggata tcgcaactta
    43021 ttggataaaa gaattcgata ttgatggatg gcgtcttgat gttgctaatg aagtcgatca
    43081 tcatttttgg aaaaaattca gagaagcagt tacagaaatc aaaccagaca tctatatcct
    43141 tggagagatt tggcattcct ctcaagcttg gctgcaaggg gatgaattcc atgcagtgat
    43201 gaattatgca tttacagatt cgattaaaga ttattttgca aaaaagaaaa tcacagcttc
    43261 acaaatggtc agtggaatga atcatcagca aatgctttac agagatcagg tgaacgaagg
    43321 aacattcaac ttattagatt ctcatgatac tgccaggatc ttgacccttt gccaaggtaa
    43381 caaagaattg atgaagtctg tgatggcctt tatgtttctt caaaaagggt ctccttgtat
    43441 ctattacggg acagaaatag ggatgactgg tgaagatgac ccggattgcc gcaaatgtat
    43501 gatatgggag aaggaggaac aagatctaga gctattcggt tttataaaag aacttgtttc
    43561 tctaaggaaa cagttgtcta agattatttc tgaaggttct actcagtggc taatagtaaa
    43621 tgatcgtgaa gataagctct atttcacacg gaaactagag ggacagatca tctatgttta
    43681 ctttaatcag tcaaaagaac cttgggtagt agaacaggaa aatgaggtga ttcttagtca
    43741 aaattgtcag ttgttagaag acgggaaagc agagatacga caatacggat ttttgatcat
    43801 gtgttgaaaa ctgttaccgg taacaacaag aaagcgtttg caaatgaaga tttgtgccca
    43861 ttataataag ggtgtaacag cacgttgaat acaaaggagg aaatgaagat gaaaaaaaga
    43921 agtgtgaaac agttgggttt agggttaatc tctttaggaa tcttaggcgg attagcggct
    43981 tgtggcaatg gtaactcttc agcagaaggg gaagccgatg aaggaaaaac gttaacagtt
    44041 tccgttgatt ctggatataa agattatata aacgaaataa aaaacacttt cgaaaaagaa
    44101 aacgatgtga aaatcaagct agtagaaaag gacatgttcg atcagttgga atcattagcg
    44161 ttagatgggc ctgctggaaa gggacctgac gttatgatgg cagcatatga ccggatcggt
    44221 gcgttaggac agcaaggaca tctggcggaa gtaaaactgg gaaatgaagc agcttacgat
    44281 gaaacagaca aagcccaagt gacttatgat ggaaaaatct acggagaacc tgcagtaatc
    44341 gagacccttg ttttgtacta taacaaagat ttagtcgata cagcaccatc aacgtttaaa
    44401 gatttggaga atctatccaa agacagtcgt tttgcttttg agtcagaagc aggaaaaaat
    44461 acaggatttt tagctaaatg gactgacttt tattattcct atggtttaat tgcaggttac
    44521 ggtggctatg tatttggtga tgatggcaca aatccttcag atatcggttt gaataatgca
    44581 ggtgctgtag aaggaatctc ttatgcaaca gattggttca aaaatgtttg gccaaaggga
    44641 atgcaagaca taaaaagtgc aggagacttc gccagccagc aattcatgtc caataaaact
    44701 gcagcaatca ttgacggtcc atggcaagcg caaacataca aagaaaacaa tatcaattat
    44761 ggtgtagcca aaattcctac tctaaataat ggacagcctt atcaaccgtt tggcggagga
    44821 aaaggatggg tagtcagcaa ttatgctaaa aacaaagacc tctctcaaaa atggcttgat
    44881 tatgtaacga atcaggaaaa tcaagaaaaa ttctttgaaa tggtaaatga aatccctgca
    44941 aaccaacaag ctagggaaac agcaaaggct aaaaacgatg aattaacaac agctgtgatt
    45001 gaacaatatg aaacagctca agcgatgcca aacatcccag aaatgggcga agtttggaca
    45061 ggtgcagaaa acttgatgtt cgatgcagca tcaggttcaa aaacaccaaa agaatccgca
    45121 gatgaagcag taaaaacaat cagtgaagcc atagaacaaa aatactgatt aaaatgataa
    45181 ccgtccattt ttataaaaaa tggaacaggt gcttcagata aaagtggcaa gagcgagatg
    45241 gaaaattatc ttgctcttgt tgccattatc ttaaaagcag tttgcggttt agcaagttta
    45301 gagggggcat tcagatgcag caagataaaa caagcaatat caacgaaaaa aaaatcacgc
    45361 tttgttcact tattccaggg atggggcagt ttgtaaatgg ccaagctttt aaaggtatcg
    45421 tttttttcgc catattcgtc ctattcattg gtcagatgct tctgggcgga tttcatgcat
    45481 tggtaggatt ggttactttg ggaagtatac caatggaaga tcattcatta tttattttga
    45541 ttgaaggcac tttgcaattc atcattacaa tcattttctt gattttttac ggtttgaatc
    45601 ttttagacgc ttatcgagta gcgaagatgc gtcaagaggg caaaaaaatc aatcggacga
    45661 tgaaggaagt catacataat ataggtgatg aaggttttcc ttatctattg acacttcccg
    45721 cttatcttct catgattttt acgatcattt tcccagtcct agttacattg ttcactgcat
    45781 ttactaatta tgacttcaat catattccac cagccagttt aattgattgg gtaggggcta
    45841 aaaacttttt tagtatcttc tttttaagct catatcgtaa tacttttttt gcagtctttt
    45901 cgtggaccgt gatttggact ttatgtgcaa caactttgca aatcaccctt ggtattctca
    45961 cagcggttgt tgctcaccag aaatttatta aattcaaacg tattttcggt atcattttct
    46021 tattaccatg ggcggtccca gcttttatta ctatcatgag tttttcgaat atattcaacg
    46081 atagcgtagg tgccatcaat actcaagtga ttcctttcat aaaccagctt ccttttgtag
    46141 atatcagtgc aatctcttgg aaaacggacc cgaactggac gaaagtagca attatcttag
    46201 tgcagggctg gttaggattt ccctatatct atgtgatggt tacaggaatc ttacaatcga
    46261 tttcagaaga cttatatgaa gcagcaaaaa tcgacggagc aaatgcatgg cagcgctttt
    46321 caaacatcac gcttcctgct gtcttcctag tggcagcgcc aacatttatt acacagtata
    46381 cggggaattt caataatttc tccatgatct atctgtttaa caatggcggt cccggaagcg
    46441 taggcggagg agctggttct actgatattc ttatttcctg gatctataaa cttacgactg
    46501 gaacttctcc gcagtattct atggcttcag caatcacgtt gattatctcc gtcatcgtta
    46561 tctgtgtgtc attgctggta tttaaaaaga caaatgcgtt caatatgggg gattaacaat
    46621 gaaaaaattt cattcgagtg ctcgattcca aaaacgactt tctcactttt ttacttactt
    46681 ttttctttta atactgtcag tcattatcat ctatccgctt ttgatcacag caagtactgc
    46741 cttcaagtca ggaaatattg cagcttttag tttagatctt tcaagtgact ggactttagg
    46801 aaactttact cggctattca gtgaaacact ttacgggact tggtataaaa atacattgat
    46861 catttctgtc ctaacaatga tcgtacaagt gactgtagta actttggcgg gctatactta
    46921 tagtcgctat cgttttatcg gccgcaaatc aagtttgatg ttctttttag tgattcagat
    46981 ggtaccgact atggcagcat tgactgcctt ttatgtcatg gctttcttat tagaagcgct
    47041 cgataagtac tggttcttga caatgatcta tataggcggt ggaattccaa tgaatacttg
    47101 gctgatgaaa ggatactttg acactgtacc aatagattta gatgaatctg ctaaacttga
    47161 tggggcaggg catttcagga tttttttcca gattgtatta ccgcttgtca aaccgatgct
    47221 cgccgttcaa gcattatggg cttttatggg accttttggg gatttcatgt tagctaagtt
    47281 cctgcttcga acacctgaaa atcagactgt tgctgtcggc ttacaaacat ttatttctga
    47341 tgtgacaaac caaagagtct ctctttttgc tgctggggca attttgattg cactaccgat
    47401 ttgtctatta ttcttctttt tacaaaagaa ctttgttacc ggcctgacat ctggcggaac
    47461 aaaaggatag gagcagagta tcatgcaaac atttccgtta aactattttt ccgcattacg
    47521 tacgccaact caagtgtttg cgggacgcaa actattatca tggcctaaat ttttcttgat
    47581 atttgtattt ttagtttcct tgatggtcat gcctgtgact cttttttatg ccaatcagat
    47641 ccaagcgatt ccattagaac aatttctatc cgttcattcg ctgatcgatg aacaaggaac
    47701 ccaaaaattt tctgaacttg aattatctga aactgggtta caagcatcgc aacaaacgat
    47761 agctgtgacc cctgaaattt tggtaggtgt gtctttatct gagaaacaac aatccgatca
    47821 tggaactttt attgattttg agaaggaaca atgggtgatc caacaaaagg ataaatctgg
    47881 tatacgccgc tatacgatga attatagccc atctttccaa ccagattctg tacgtacgcc
    47941 cgaagatttc caacgatttt tagaaagaga attttatgcc agtaatcgtc caacaatcat
    48001 tttatcttat agtttaagtc ttggtcttgt cctatttgtc atgacgagtc tcattttatt
    48061 cggggcttcc tttttcttgt ggatgacaag aaaaagccaa ctttcatcca ttcatacatt
    48121 caaagagagt gcaaatttga tgctgaatgt tatgggaata ggttccatgg cagcagcagt
    48181 tgtagggttt atccactttg actttatctt gatgctaagt gtccaaacaa cgataactgt
    48241 cttgctgctt ttatgggttt ttgtcaaaac aagattcaaa gataagcgtg tcgaatgaca
    48301 agtttaagcg atttatgcga caatgaatgt gaaaaaaatg aaaaataaag tagtttttga
    48361 aaaatttctg aaaaaattaa acttcttagt tgattctttt cttttttagt attaacgtaa
    48421 tagggtagtt tttttaagga ggaaaatcat gttagagaaa actttataca atcaattatt
    48481 ttcacgttct ttctcgctcc ctgtcgaagt aaattattgg gacggtacaa cgaaacaata
    48541 cggagatacg gacactccta cccaaatcaa aatcaatatc catgaagaaa tcccaatgaa
    48601 agagattaca agtaacgcat ctttggcact aggagaagcc tatatggatc atcgaatcga
    48661 gatcgaggga agtatccaag cattgatcaa cgatgcttat cagcaagcaa acagcttttt
    48721 acgaggaaaa gattatctaa aatggctgcc aaagaaagaa aaacatacga aacaaaaaaa
    48781 caaagaagat atccatgcgc attatgactt aggtaacgac ttttataaac tttggctaga
    48841 tccaactttg acttattctt gtgcttattt caatacacct gaagatacgt tggaacaagc
    48901 acaaatcaat aaagtccatc atatcttaga caaactcttt atcaaagaag gcgaaacgtt
    48961 gcttgatatc ggttgtggct ggggaacatt gatgttcact gctaccaaag agtataatgt
    49021 caaggcaact gggatcacac taagtgaaga acaatacgat ttgattacca aaagaataaa
    49081 agaagaacat ttagaagata aatgccgtgt attattaatg gattaccgcg aactaaaagg
    49141 cgaaacgttt gatcatatca caagtgtagg tatgttcgaa catgtaggtt ctgaaaatct
    49201 tgaaggttat ttcaaagtgg taaaagattt attgaaacca aaaggaacag ctttgatcca
    49261 tggaatcagc cgacaacaag gcggtgcgac aaatgcttgg atcaataaat atattttccc
    49321 tggcggttat gtccctggtg ttactgaatt agttgataat attacaaaaa acgatttgca
    49381 actgattgat ttggaaagct tacgtcgtga ctaccaattg acattagaac attggacaaa
    49441 gaattttcat caagtccaag atcaagtcac gaaagaaatg ggtgattcct tctatcgaat
    49501 gtgggatcta tatttacaag cttgtgcagc ttcatttgaa tcaagtaata tcgatgtgat
    49561 tcaatatttg cttgttcaac ctggaaacaa tgacatccca atgcatcgtg cataattgag
    49621 gcaaaagtac tgtttaaaga ctatttaaaa agaagctaag ttttatgatg ctccagaagt
    49681 gaactttttt gtctaacttc taggattgtc tctaaagctt agtttctttt tctttatatt
    49741 ttcataataa aagtttggtt ctttaatttt taaacatgac agaaaatttt ttcttcgtta
    49801 tacttgtaat agaatactat tttataacaa aggggatcga atcaatgaaa aaaactgtta
    49861 tttatactag tttatttgca gctttgtttt tagctgcttg tcaatcaaat accaatcaag
    49921 gcacatctgg gaatacgtct tccagtagcg aacagcgctc ttcacaggta tcggactcct
    49981 caacaaaagc aagcacatct tcgtctagcc agacaaaaag ttctagtgaa gcaagtactc
    50041 agtcagatat caaagtgtct gcagctcgtg cgatcgaatt gttccaagat aaatacccag
    50101 atactgcaat tactagtttg gaattagata gtgattgggg atcttacttc tacaagatag
    50161 aaggtgtcga tgatcaaaaa gaatacgaag caagaattga tgctcaaaat gaaaaaatgg
    50221 agacagagaa tccagaaact ctagatcgag atgaacaaaa tggtcaaaag aaaaaagaag
    50281 atggtctgga tgttgataaa ttgatcacga ttgaggaagc agcaaaaatc gccgtcgaca
    50341 aagtaggaag cggtacggca acagattggg atttggataa agatttaggt actacttatt
    50401 gggaagtcaa agtaaaagac ggcaatcaaa gtacatcagt caagatcaat agccaaacag
    50461 gcgaagtact gtcaactgaa caagatgatt aagaatttct tcggaaaaca agagttgatt
    50521 tttcttttag tcatgataca ctagacaaaa taacttgttt gaaggagcga tttacttgag
    50581 tttagaaacg acctttgcca atcatatctt tgccaatcct ttgatgaatg catctggtgt
    50641 ccactgcatg actacccagg aattagatga actggctcat tcagaagcag gagcatttat
    50701 cactaagagc tgtacgatca atgaacgaaa aggaaatccc gaaccacgct attttgatgt
    50761 tcctttaggg agtatcaatt ctatgggact tccaaactta ggtttttctt attatctaga
    50821 gtatgctctt gcgtatgaaa aagtgcagga aaaccaaaat caaccgttat ttttttccat
    50881 tgccgggatg agtgtccagg aaaacttgga aatgcttgag aaaatagaaa aaagtgggtt
    50941 taatgggatc actgaattaa atttatcttg tcccaatgtg cctggaaaac cacaactagc
    51001 ttacgatttt gaggccactt atgaaacact gaaagaagta ttcagcatct tttctaagcc
    51061 gctaggaatc aaattgcctc cttattttga ttttgctcat tttgatcaga tggctgatat
    51121 cttgaatcag tttccattga cctatgtcaa tgctatcaac agtgtgggca atggtttgta
    51181 tatcgataca gaacaagaag cagtagtcat caaaccaaaa gaagggttcg gcggcattgg
    51241 cggagagtat atcaagccga ctgctttggc gaatgttcgc gctttttata cacgtttgaa
    51301 accagaaatc caaatcattg gtacaggtgg gattcggaca ggacaagatg catttgaaca
    51361 tctgctatgt ggcgcaagca tgttacagat tggtaccgag cttcataaag aagggccgga
    51421 aattttttct agaatcataa aagaattaac gcagattatg tcagaaaaag gatatacgtc
    51481 aattgacgaa tttaaaggca aattacgcac catttcatag caaagagacg aaggtcatga
    51541 ttgatcttcg tcttttttta taaaaaacac tcgtctgttc attcctgtgc aagcgtactc
    51601 attgtatgtt agacgaactt gtgggaaaat aacagaggca aaaaggagga taatatgaat
    51661 gatgcaacag aattagcaaa agagcttaaa gaaaaagaat ttagttttca agaactagtt
    51721 acggatatac agaaaaaaat aaagaagcaa aatcatgaat tgaatgcgtt tgtcgcgctt
    51781 gaaccattcg agagtaattc ccacgagcta ttgattcccc agtcagaaaa agggccattg
    51841 gctggtatcc catttccttt aaaaatgctc ggtcaagaaa aaaagggatg gctagctact
    51901 tcaggatctc gtttatttga aacacaccga gcttctcata catctaatta tgtgcggcaa
    51961 gcagaagcga taggtctagt tccttttgga caaaccaatg cgccagagtt tggtttcaaa
    52021 aatattacag acccagtcat ctatggccca gcaagaaacc cttggaacct tgaatgtaca
    52081 ccgggcggat caagtggagg agctgctgct gcagtttcat caggaatcgt tcctttagct
    52141 ggtgctagtg atggcggtgg ttctattaga atacccgctt cgttttgcgg attgattggg
    52201 ctgaaacctt cgcgagggac gatgccagtt ggtccatatg cttggagagg atggcaagga
    52261 gctgctattg atttcggctt gacagtttct atgagagaca cggaggcttt attttatggg
    52321 atgagaagta tccattccgg tgcgccatat caagtatcgc cagttgagtg gcaaacacat
    52381 ccaagaaaaa aacggttgaa gatagctttt tgtgttgatt caccgatcca ctcacagatt
    52441 agtgaagagg cagtacttgc tgtaaaaaaa gctgtagatt tccttggaaa agaagggcat
    52501 gatttgacag agattcctta cccactagat ggtcgtaaac tgatcacgtc ttattatcag
    52561 atgaatggcg cagaaacagc agcaatgatc cgctcgattg aaacaggcat ccagcgagct
    52621 gtcagaaaag aagaacttga acccatttct tggactttgt tccaatatgg tcagaagctc
    52681 tctgccgcta cttacatcga ttctctgcat gtatgggatg aagcagcaat tgtcatggaa
    52741 aacctacatc aagaatacga tctattcctt tcaccaacga ctgcatttac cgcccctaaa
    52801 atcagcgaag atttgcaaag tgatgcgata agagcacaga tggaacacat cagtgattgt
    52861 tctgaagtag aaggtttaga cgttgtttct tccatgtttg aggagagctt gaagatcaca
    52921 ccttatacac agttggctaa tctgactgga caaccagcaa tcagcttgcc tactcatttg
    52981 acaaaagaaa acatgcctct aggaatccaa tttatggctg ccagaggaag agaagatttg
    53041 ctatttcaaa tcggctatct atttgaagca aatcatcagt tccatttacc aaatccatgg
    53101 aaatagcaga aacaaggaga agaagaatga agaaaaaaag attgtttgct ctaataacta
    53161 gtgccttttt tattttaagc gcttgcggta cagctgccgc taatcaggaa gataacagct
    53221 ggagtaatgt agaaaaaaag aaagaattag tagtagcgac ttctggcaca ctgtttcccg
    53281 catcttatta taatgatcaa aatgaattag ttggttatga tgtagatttg gcaaaagagg
    53341 tggctaaacg gttaaatgtc aaaatctcct ttaaagaata caacgtggat ggacaaatca
    53401 cttctgtttc aaaaagagaa tcagattttg ctgcgaacga ttttggtatc acaaaagaaa
    53461 gagaaaaaaa attctccctt tcagagactt tcaaatattc atttgacagt atgatcgtac
    53521 gaaaaaaaga tgattcagga atccactcac ttgaagatct taaagggaaa aaagcagctg
    53581 gcgaacccaa tactagctat atgcgtttag cggaaaaaca cggagctaaa ttagtcacat
    53641 atgacaatgc cacgaatgat cagtatttaa gcgatgtagc aaatggtcga acagatgtta
    53701 ttctaaatga ttattatctg cagaaaatgg ccgtatctgc tttaccagat atccctgtaa
    53761 aaatactgga gaacgtttat ttcaatgaaa acgaaagtgg ttttttattg aaagaaggaa
    53821 atgatgctct gaaaaagaaa atcgatggcg tattaaaaga gatgaaagaa gatgggacga
    53881 caaagaaaat atctgaaaaa tattttcaaa ctgatgtttc agtcaaaccg aaagaaacga
    53941 tggctgagtc ggtgagtgag tgatgtactt tcctctaatt gatttttccc tcatgtggac
    54001 ttcacttcct ttcgtactgc aaggcttggt atacacatta gggattggct tcgtgtcgtt
    54061 tcttttagga aatctggtag ggttgctttt gacagtttta ggtcttttgg attggctgcc
    54121 tctgaatgta tttattcgtt tttatttatc gttctttaga ggcattcccg cactagtcct
    54181 gctgtttctg ttatattttg ggttacctta tcaattaagt gccttaacgg cttcagtcat
    54241 ctgctttacg attaccagca gtgcatttat tggtgaaatc tatcgaggat cactagcagg
    54301 cgtttcttca ggtcagtggg acgctggttt agcttctggt ttttctttct ttggggtatt
    54361 gaaatatatc gttttgccgc aagcttttcg aatctcagta ccggcactca gtaacgtagc
    54421 tatggatcta gtgaaaggta cttcgttagc agcaacaatc acagtgccag aaatatttca
    54481 aaaagcaaag atcataggcg gtcggacatt cgattatttt tctctctata ttttagttgc
    54541 actgatttat tgggtcattt gtatcgggat cgaaaaagtg caacagtatt tagaaaaacg
    54601 ttctatgcat cagtcaatct gaaaaataga aatggtgcta gcgtggctgg caccatttct
    54661 attttttgtt tcttgtatat tctgcctagt attttaaaac catttcatgt aaaatgtaaa
    54721 tcgaaaatat gttcggaggt agaaaacatg aagaccccgc taaccatcga aatagaatcc
    54781 tcgttatacc aatattgcat cgagcaaggc ggcttggttg tagaagaagt cacgatgcct
    54841 gatgaccaag gaattgtaga tacgcttagc tgcttgacaa aatttgatgg aagccgagaa
    54901 tggcgatgct atgagctgaa agtgacaaaa gcagattttc gctctacagc caaactgtca
    54961 tttgttggac attataatta ttttgtttta ccaagtaaac tatatgaaga agtaaaaact
    55021 gaaatcccta caggaatagg tgttttagtg tatcgtccgt acacggttga agaggagatg
    55081 cctgcctctg gtacatttct gattgcgaaa aaagcagcaa agaaagagct tgctgtaccg
    55141 gaagaagctt tgacaaaccg cttcatggct tctttattca gggaagtaag aaaagcaaag
    55201 caaatggagc gaggcgtaaa atattttcca acagatcaat tgtacaaaga attgaaaaga
    55261 cgttcagaaa atcaagatcc attttctaac gaacgatatt atgaacggtt tttagaggaa
    55321 atagctagtc aggcaatcaa tgagttgcaa gaagaagtta tcgcgcttca gcatgattat
    55381 gaatatttga ggcaacaaca gcagatccgt cggttgccta cagagccatt ggagtgaaaa
    55441 atatgtacta tccttattta agaggaaaac aatttgacct attagcatta aaagaagcac
    55501 tgtcgcgagg attgctctcc aataaaatcc aaccagtgat cgaaccagta cgtgattccg
    55561 ctacgcttaa aaatgtgatc gaattatttc aaaaaaagca ccatcgaatc gctgtgatcc
    55621 aaaatcccca agtaggacaa ttcaaattat tcgatcagca tgtgcacagt tggacagtca
    55681 aagaaaatag ctctgtcgtc cctgcacaga tcttaacgcc agaaaatctc agtgaagtac
    55741 tggattcacc tccagcgttt cttgttttcg atggacagca ctacccaaaa gatacagagg
    55801 tgtggaaaca gttggcagga gttgattcga aatttttgat accagatact agtcgctttc
    55861 gcatctggct gcccgaaaac aaaattgtga tccgtgactc ttttcaaacg agaaagcatg
    55921 ttgaaagtta tgcagataaa acagatgact tttttagtga tgactatctt tttttccacg
    55981 cagacggata tattggattt tctgatttca cgatcgaagg tagccgttat ttcgataaag
    56041 ggttccctag ccgagcgctt gctatccatc taacgtatat tgatgcttac ggcaatatac
    56101 gcgtgaaaca ttttgtctct gacagtaacg actctgctaa agaccaagca ttgaagtttc
    56161 ttgaagcagg agataaaatg aaaaaatgga tcatgcgtca ccatcatcaa ctcttgatta
    56221 cttcaggaat gattgaatta ttggctcttt accagcaaca aaaattccct ggactgggag
    56281 tgttgaaaaa gtggtcactt atgcatcatt tggaacttat tagtcacctg ctagatcatc
    56341 caaaagattg gcttaaatat tattcaaaag ttcaggaact atatgaagtg atccaatgat
    56401 tagataatgt aaatgaaaaa ggaatagaaa agaggaaact ggatgtatat tcggcaaaca
    56461 aggaaagaag aagtagaaga agtggtagaa atcattaatg ccgcagcgga tttcattgct
    56521 tcgcaaggct ctcctcaatg gcaaaacggg cagaaaccaa caaaagaaac gattttgaac
    56581 gatattgaaa gaaaagaaag ctatgtatta atagtggaag atcaaattgc tggaacagct
    56641 gcattagtct caagcgtgga tcccgtttac acaaaaatag atggtagttg gaaagaatca
    56701 actggtcctt atttatcgat tcatcgtgtc gctttagcag aaagtactcg aggaaaaaaa
    56761 ctagcaaaac cgttcttaga acatttgttg acgattgcag ccagcaaaaa tatccaagat
    56821 atccgtatcg atacttatcc agctaatgaa ccgatggaaa agaccatcta ttctgctgga
    56881 ttcacttatc aagggatgat tgaatttcct tttgaacacg gcgaaagaaa agcatatcaa
    56941 ataatcatca attgactata aaatcatctg agcaaagata aagacattaa cactactgaa
    57001 aaaaacaatc atatgatagc caagaggttg taacagaagt ggccagcttc aaaaaacaag
    57061 aaagaatttc cgaaaatgct tctcaaattt ttgtggaatt ttagcgtatt ttacgaaata
    57121 gctgctttct gaaactccct tcatcgagag gtcgtgacat agcttttgtc acgacctctc
    57181 ggcttatcct acgagtatta ttcgatacat ttatatcaat aatccttttt ttattttatc
    57241 tgcagcttct tggaacgctc catcgtttga cacgactaaa agtgagggga tagctttcaa
    57301 acgtttcaac tgtgctaaat cttctacata aaccgccagt ctggctgaaa tctgttcaga
    57361 tgaatctcca cgttttttca ggcgcgattg caatgtttct ttagagatcg taataaagat
    57421 aggacggact accgtcccga aatactcata taaatgccaa aaaccatttg ctgtcaaaac
    57481 ggcgtaacaa tttcctactg tcagtttctt tttaatttct gctttactgg aaccatattt
    57541 atgttgagca tattggtccc actctgcaaa ctcatttccc ttgatcatct gattgaatgt
    57601 attgtcagaa acaaaataat aatcctcttg atctttttcc ccaggtcttg gtgatcgatt
    57661 cgtgtaagta atgatctttt gttccggctg aaaaatagct tctgcgagtg aagtttttcc
    57721 agaaccagaa ggaccgataa atacatagca ataattcatt gtcatttctc ctttgatgat
    57781 tgaacaagtg aaaaagcttt ggaaaatatt tccaaagctt ttaaaagtaa ctttgctttt
    57841 ttgtatatta taccagcaag ccagtcacaa tagcacttaa aaagctgact aaagtcgctg
    57901 ttagcaaaat tttcaatccg tgttttgcga caagatttcc tttttcttca tttaatccct
    57961 tcatcgcacc agaaatgatc ccgatagaag agaagttggc aaatgaaacg aggaagacag
    58021 atataattgc tgttgtacgc tcagtaaaat ggaaattacc atttgctaat tctgtcatag
    58081 caacaaattc atttgtaacc agtttagtcg ccatgatact accggcatcc actgcttctt
    58141 tccatgggat tccgctgatg aaggcaagag gggcaaagat gtaacctagg atctcttgga
    58201 aagtgatgcc gaatatcccg tggaagatag cattgatcat ggcaatcaaa gcaacaaaac
    58261 cgataagcat agcagcaacc gtgatcgcca catggaaacc atcaagaatg tattctccca
    58321 acatttggaa aaatgtctgt tctttgtttt cttcaatgat cagctcatct tctttttctt
    58381 ctaatacata gggatttaca attgaagcca gaatataacc gccaaataag ttcaaaacaa
    58441 gggcagtgat cacatattta gaatcgatca gtgccatata agatccgacg atcgacatag
    58501 aaactgtaga catagccgat acactcatgg tgaacaaacg tttctcagat aggaagggta
    58561 attccttttt tatggaaata aaaacttccg attgtcctaa aatagcagaa gctactccat
    58621 tataagattc caatttgccc atcccgttga ttttacttaa tcctagacca acagctttca
    58681 taaataatgg aagaatcttg atatagcgta gaataccgat aatagcagaa ataaatacaa
    58741 ttggcatcaa tacggaaaga aagaaaggtg cacttccttt atttgctaca ccggcaaaaa
    58801 caaagtcaac accttgccca gcaaaagcaa gaagatgatc aaacattttt gccaatacag
    58861 aaacaaccgc tgtaccaaaa gttgtacgta ataatacgaa accaagaaca agctgtaaaa
    58921 cgaacatcac aattagtggt ttcaatttga ttgctttttt gttactgctg atcaaaaaag
    58981 caagccctaa tgtgacaaat aaaccaataa tacctaataa ataactcaaa taatcactcc
    59041 tattccaaat tccgttttat tttacctgtt ttgcaaataa aatactaatc tttagtgaaa
    59101 ttttcattac ttttttagag tgaaacgcat tcattttcca ttagaaaata aagcgaacaa
    59161 aataaaaggt ttaaaaagtg taacgtctta gtaaagtcta taaaaaaacg aaactatcga
    59221 ttaagctaat agtttcgttt tcaacattta taagtaaact ttttctattg gtaatataga
    59281 ttttcggaag ggtagacagg tgcgcaggta atatcgattc ctagtttttt caatgtttgt
    59341 tcatcatttt tattgaccat cacagtagag tgggcttgtg ctccctcaag ttcagctagt
    59401 ttatcataag caacttgagc gattggattc gttactgcac tgattgataa agcgatcaag
    59461 acttcattga tgcttagtgc atccaaagag gaatgaagtg tctttgattt caatccttgt
    59521 attgtttcaa gaactaatgg tgacaataaa ggaatgtcat ctgcgatatg ggctaaatgt
    59581 tttactgcat ttaagataac tgccgctgac gcatccatca atgaacttgt acgcccggta
    59641 atgatgactt catctggtaa ttcgattgca atcacagcag aaggatcttt tgtaggactt
    59701 tgcgcttgaa tcttatctgc atagtcatgg gcagggacta ccacgtttcg gtcttcagga
    59761 cgtaaaccga cttcttccat aatcagtttg attcgattaa ctgcttcttc gtcgatcaat
    59821 ccttttttga agtcgcattc tgtttggaaa taacgacgga tgatttcttg tttagaggct
    59881 tcttggacaa cggtatcatc cacgatccca aatcctacac gattcacacc catgtcagta
    59941 ggagattgat aaacagattc tttaccagta attttttcaa taatacgttt gattacagga
    60001 aatgtctcaa catcccgatt atagttcaca gctactttat tgtaggcatc aaagtggaag
    60061 gaatcgatca tattgacatc ttttagatcg actgtggctg cttcataagc aatgttcagc
    60121 ggatgtttca acggaacatt ccatactggg aaagtttcga acttggagta accagctgca
    60181 tgtccttttt ggctttcatg atacagttgg ttcaaggaag tagcgagctt tccacttcct
    60241 gggccaggag ctgtcactac aacgattggt ttagttgttt caatatactc attttttcca
    60301 aaaccatctt ctgagacgat tttttctacg ttgactggat agtcttcgat ttctgagtgt
    60361 ttatagacct tgatgtttcg tctttctagt ttattgataa agactttcgt ggcaggctgt
    60421 ccactatagc gagtgataac gacactgttg attaataagc cataaccacg taattcatcg
    60481 attaaccgca agatatccat atcataagta atcccataat caccacggat cttattacgt
    60541 tcaatatctc cagcatagac gcaaatcagt atttcagctt gatctttcaa tttttgcagc
    60601 aatttgattt tcgcatcttc ttcaaatcct ggaagtacac gtttcgcatg cttgtcgtca
    60661 actagcttcc caccaaattc taaatataat ttgtcatagt ggttcactcg ttccaaaata
    60721 tacgctgact gctcttcaat atatttttct gaatcaaaac caattttttt cactaggttc
    60781 cctccttaag tactaaagca ttttttcatt tttttgagga ttcgtcaact ggaagagcga
    60841 aattcctcaa aaaaacaaag aaaatggttt aaaccaactt gtctagatag ttattcattg
    60901 gtatgatagt ttttgataag gaaggtgcta catgaaaaaa agacagaaaa agaaaaacgc
    60961 atataaacac tatatccgtt cgatttttac cggttatgaa agaatgcttg aagatcccga
    61021 attggaacaa ttgacgttta cttatctaaa cgaagaaacg cagctaactc gtgatgagca
    61081 tcagcgcatt catttcacaa caagagatct gcctagtaaa taaaaaataa gataaaggtc
    61141 atcggattcg attctgtcct atcagatgga aagactagtt gatcgaaaaa aaggctgtac
    61201 caaattttgg tgcagcctgt tttatggagc attgcccgct cttttttata acaatttttt
    61261 acagaaaatg gtactattga agtattaata agtgaaaaac agatgagtaa gaggaggcaa
    61321 gtagtatgaa agagccagat acaaatgcaa attatgagcc agacgaacta gaggagacga
    61381 atgcggatca ggatacagga caagaatcgt cttcttttta cgatatcaaa gaaaaagcaa
    61441 gcatgaatct ttcccaatta ttagaagact tacaggcttt tgaaaaagct gtccgcgaag
    61501 aagatatccc tgagatctac cgtttataca atggccgatt gaatgaagaa ttaaaaagaa
    61561 gctcaaatga aaatcacgag atcgatcaat tgctgatgcg taagatccat gatcgttttt
    61621 tacaggaatt tccttttatg gaacaagtag aaaacatctc gccaacgatg agctattaca
    61681 aaatcggcac ttattaccat gatcggccca cggtcggtat tgatgcaagt ctgccagaaa
    61741 tttttgtttt gcctaagatc gatgaagagt gggagaaata ttcccaaccg gagtcaacgg
    61801 actatgacaa aaaaatcaat gagctagacg caaaggtgat cactgctcag acagaaattg
    61861 aacgtttaga tgaacaaata aaagaaatca atcgacaaat gactgatttg aacgacaata
    61921 aaggattctt gaatcgtaaa aaaatcgagg aagaaattca agaattagag aagaagaagc
    61981 aagtactatc taatgaaaaa ttaggctggc ttccttatat tgagacgcct gaaacaatcc
    62041 agcagcaaaa agaagcattg aagcaagaag caagagcaga ccagcttcgt gcagccatcg
    62101 tagaaaaaga acaaaggcaa atcagacgct atttcggtag caaagaggga ttcggtcaag
    62161 caatccatga attcttgatg aattatcttg gcaatgagaa tcctaataat caatctaatg
    62221 aaggaggttc cgagtatgaa tgaacacgat gaagcagaat ggacaaaaaa tgaagaacaa
    62281 acagaagatc atgaagaaac gaacacttca ttggaagaac aaacaagtaa tcctctggat
    62341 gacacagaag aaatccctat gctagaggaa acaaaaatac acgaaaaaga aaaagtaact
    62401 ccgacgaata cgaatactgc agaaaacacc catgcttacg aaataactga aaatcccggg
    62461 tatttagaag gaaaagagca agatcatcaa gatatcgtca gtttgattga aacagcggaa
    62521 gcagaattag cggatttacg tatgagaaaa gatttgatcg aatcagattt tccacaatta
    62581 ttagaatcat atgatcgttt tatattgaat gaagaaccaa tctccacgat cggaaagaca
    62641 agcctgatcg aatacttcac gtatgaatta ctgaaaccgt tgaatgatat tggtctggaa
    62701 cggtcaaatt cagcttatga cacatggaaa acaagacatt tttcgatcaa ttatacgttg
    62761 aatggacaaa acgaattagt cttttccttt gtattaccga ccatcgatca acgataccaa
    62821 gtggaatcga tgagtctcct aaacgtatca ccagagacaa tggaaatcaa tgtccaagat
    62881 gaccgggtgc tttctttgat ccgacactgg tcagtggatc gagtattctc tgcagggcag
    62941 atcacgatat tcaatcacaa attgaatcaa ttattagccc atgcaagaac acttggtttt
    63001 accgttaatc agacattact agacaatacg aagccacttc gtttgaatct tcagagcgaa
    63061 tttgaattga cgaatgaagt attggatgat atttttatcg tggcaatgaa aaatccatcc
    63121 tacgatatgg aaaagatcaa cgaaactact tatcaagtac gattagataa aggacagtct
    63181 ttaacgataa caacaaacga aaaagatcaa acagtcttgt cgatctcatc tggagactac
    63241 ccacgatctg tcattgattt ctttatcaat tacgaattct tggtcccatt aatggtgaga
    63301 aagtcctgat tggataagtt ggattaccag atgtcattca gataattctg ttccaaagga
    63361 cttgcggcag ttatccagaa taattgtcac aagtcttttc tgtatgtact aaatggacgt
    63421 gaataacttg ctgatgaagg agggatttca ttaggaatgt ccacaaataa aatagcacag
    63481 cttagcttat tgtctgctgc ttgtgtagca ggtaggatcg cttttcaatt tattccgaat
    63541 ttccagccaa tgactgctat ttttttattt atagtacttt acttaagctt aaaagatgcg
    63601 ttaattgtca tgagtctttc tattgcgatc agcagttttt attttggtgt tggaccatgg
    63661 gtcatcggcc aatggatcag ctatactgtt gttctgagtt tgttttcaat cgtctgcttg
    63721 agaaaaacag tgcaaaggaa tctatggtta aagggagtct gtttttttct ggcgggcatg
    63781 gtttacggaa taggtatgac cgtttttgat acgctgttat atcgtttgcc tcaaccttgg
    63841 atctattacc ttcaaggggt gtcgtttgat ttgatgcaca gtataggcaa tgttgtgttc
    63901 tttcttgttt ttctgccagt cgtcaaacgt ttttacaatc attcaggagg aaaaaatgaa
    63961 aaaaataatt tatagtttgc ttgcaatgag tgtcagtata ctcattttga caagttgcag
    64021 tacaacctat acacaatcgg cttccaacca aaaggaggaa gtaaaaacat cgcaaaaagc
    64081 aacaatcacg ttacaagaaa atggtcaaaa cttttcggaa aaagaagtgt tcttcaaaaa
    64141 aggcgatgat ttactgagta ttttgaaacg aaattttgaa gtcaaagaag ataacggctt
    64201 catcacttcg cttgaagggc actcacagga tgagaaaaat caactctatt ggatgtttac
    64261 tgtggacggg aaaagtgcta ctactggtgc caaagagatc aaattgaatg atggacaatc
    64321 ggtcattttc aatttgtcta aactataagt ttcataaaaa actgttaaag ggcgaatatt
    64381 gccataattc agtgagttca gttgatgaaa gaaggaaaat aacatggaaa attttaaaaa
    64441 aagcggaatg ttttccgtaa ttgctgctct aggggctaat attctagttg ctatcagcaa
    64501 atttgtcggc tatgcaatca gtggcagtgc cgccatgcta aacgaaagca tccacagtat
    64561 cgttgattgc agcaatcaga ttttgctgtt attcggtgat aaacaagcca ataaaggaca
    64621 gagcgagttg catcaatttg gtgaaggacg agcaaaatac tttttcagta caatcgtcgc
    64681 aatgatgctt ttcttcggcg gtggggcact tggggtgatg gaagcttttg aaaagttgac
    64741 gcatccatcc catgaagtgg gtaatccgat cattgttata gcaatactgt tatttggttt
    64801 ggtaatcgaa ggaagttcgt tacgcgtggc aatgaaagaa atcaaagaat tgaatactga
    64861 aaaactttcc acgatgcgct ttttgcgcga aagtcgacac agtgaaatac tgattatttt
    64921 tacggaagac ttgtgtgctg taatcggttt ggtcttggct ttagcaggaa caatattgac
    64981 ttatgcaacc gggattgctg catttgatgc gatcagcgga ttgctgatcg gtttcttgct
    65041 gatgggagcc gccattttcc tagcaaaaga attttatagt ctgatcatcg gtgaaagcgt
    65101 gacagcaagc gatttatcaa aaatcaagtt agcttttgaa cgtcctgaga tcagtcggct
    65161 gatcgatata aaaacagttc atttaagtcc aacggagatt ttagttgcag caaaagcaga
    65221 tatcgttggt aacaaagaga atgaagctta ctcggtcatc aatgatattg aaaaaaatat
    65281 gcgtcagtct cttcctgaca agaagatgta tatatacatt gaaacagata aatatgaccc
    65341 aaattattct cgtaaataaa acgtttattc atgaattgaa gcaaaaatga gaaaaatata
    65401 atcttgtttt taaataaaac ggctacaatt tagcgaattt atgttacaat gagtccgctg
    65461 tttatttgtt gtaattaaag gagatatttt aatgaacaaa aagaaaagtg cgccatggct
    65521 tcaagctttg catgcagtga tgccactttg tataagctat ataccagtag gattagcttg
    65581 cggagtattg ttgcaaaaag ttggatttga tcctctctta tcaggactgt tatcgattct
    65641 ggtcttttcc ggtggcgcgc agtttttagt tgcatctatg cttacgacgc aagcatcatt
    65701 tgcaacgaca ttattgatgg ttttcttttt agaattacgc tatacattat taggagcaag
    65761 tcttgccggt tttatgaaaa aagaaaagcg gacgtttttg gctgtctttt ctcaatcact
    65821 gaatgatgaa aactatgcgg tgaactactt aaagttttca actgaccctt cctggaataa
    65881 aagaaaggct ttatatgtca attggttctc aatgactgca tgggccggta gcaatatgat
    65941 cggtaatttt ttcggctcgg tgattcgagt agatgctgac cttgtccatt ttgctttgac
    66001 ggccatgttc attttcatgt ttatcatgca gatgaaaagc gcactactga tctttacagg
    66061 tttattctca ggcgtattag gggtcgtatt catggtatta ttccaaaata ctttcggatt
    66121 gattgcagca acagtcattg cttcatttgc cggatttatg ctggagaagg cattcaaaaa
    66181 agaaaaagct aagaaactaa gagaccgcgg taaagatgtc aatgagccgc tcttcaactt
    66241 cccggaacag gaggccaatc accatgacta aactatattt gctactttta gttgcttgtc
    66301 tgttcatcgt ggcatatatc ccgcgcttgt tcccgatgct ttattttact catcgaaaag
    66361 tgccatcctg gttcagtgac tggatgaaat atgtaccggt ggcattattt gcggcgttag
    66421 cttttaaaga tgttttcatt actcatgaac atctggatat tgcttggaat atcaaaatag
    66481 ctgcaatgat tctagtagca ggcgtggctt acaaaacacg ttcgatggcg ttgtcagttt
    66541 tgacaggact tgcgtctgtc tttttgctgt ctatgcttta aactgtttta ttttattaaa
    66601 aacagagatt gcgacataag ttgttcaaac tgatctatcc gaactgcatt tgcaacagtc
    66661 aattctggat gcaagtttat tcgtagggat aaaaaagatt agtgttggct tatgatgcaa
    66721 gctctttttt attttaggaa ttagagaggg atttgtcctt ctatgatttt ttgctataat
    66781 agggaagatg tttattttat gaaagaatag ttgagggaat ttaaatgaat aaaaaattga
    66841 tgcaaggaac attttggcta acctttgcta atcttctatg taaagtttta ggggtagtct
    66901 atttgattcc ctggctaagt atgatgggga acaatcaaga tggtatgctt gctacgacat
    66961 tgtataatgt tggctatctt ccttatggat tgttcctgat gcttggtaca gtcggatttc
    67021 caaatgcgat tgcaaaaaaa gtagctgtag ccacaaaaga aggagataac gctgcttgtc
    67081 ggctgatttt tagaagtacg atcaatatca tgtttgtcat cggtatcgcc tctgctgcac
    67141 tgatgtattt atttgctcct ttactagccg gaatcagtcc gattgctaac gttaataatg
    67201 ggatactagc tatcagaagt ttatgcccat ctttgattgc tatcccaatt cttagtgcaa
    67261 tgagaggata tttccaagga aaaaatgatc tgcggcctta tggaacttct ttgattatcg
    67321 aacaggctgt gcgagtgatc gttatcttgg ctggtactta ttacttgcgc gtattgactg
    67381 acggaaccat attagaagct gtattgatca gtacagtcgc ttctttcttc ggtgggttag
    67441 ctgcgattat ccatatgtat atcgtcggac gcagaaaaga cttcttcgaa cttaaagatt
    67501 ttttgatttc cagccgttat ttcgaaagag aaaaccgatc tgcctctgtc tctatcattc
    67561 gggaaacact accgtttatt tttgtcggct cagtcattac tatcgttcaa atgatcgacc
    67621 aagtaacgat gaaaccactg cttcactttt tccgtccaga gatcgcagat cagcaactgg
    67681 aattcttatt cagccgtgct tctgtcaatc caaataaatt gacattgatc ctgatctcca
    67741 tggtaggaac agtagctata agcagcttgc caatattaag cacgatgaag aaaaaagatc
    67801 ggatacaaat cgaaaaaaca gtaggagata gtttttcgat tgcgttattg attcttcttc
    67861 cttcattgac tggaatgtct ttattagctg gaccactata cacattgttt tttggctatg
    67921 atcctgaaag tgtcggatat ttccaaatgg ctttgctagc ttctttgttt ttctctttat
    67981 tcacgatcct atctacgatg cttcaatctt tgaatcacca tcgttttgcg atccgattaa
    68041 ctgtagaagc aattatcctg aaagtcatct tccaagtaat cggattagga ttagttggcg
    68101 gatacgggat gagcttatca aatggcttag cattcggtat tgtctttgtt cgcggctatc
    68161 actttatgtg caaagaatac cgaatagcac ctttagcaaa gatcagcaat tttttcttaa
    68221 aaactttccg ctccaccctg attatgcttg cagtatgttt tgccgttttc tttttgctga
    68281 atcagcgatt gtcaatggaa tcaaaaagct atgcaatgat ttattgtgtg gctgtaggaa
    68341 gtattggcgg cttagtattt ttattttctc aattcggaaa aaatgggttt cgaatgttga
    68401 aaaatgttaa acatcatagc agaagcaaag aataggttaa ctaatcgttt tttgctgtca
    68461 cttgaaaaac ggcaactcgt atgattacgc ttgtcgtttt tctattttcc aattttagtt
    68521 tcatatctaa tcttacttat gatatagtta taaggtctgg aaaaattctt tgaaacgagg
    68581 aaattaaaca catgaaactt gcagtagtta cagatagttc ggcatatctg ccggaacgta
    68641 tcaagaacca tcccgactta ttcgttattc caatcccttt gattatggat gggaaaattt
    68701 ataacgaagg aatcgatatc gaagcggatg aatattatgg acttttaaaa aacagtaaag
    68761 attttcctac tacttcacaa cctgtcgtag gggaagtttt gggtttatat gaggaattga
    68821 aagagaaagg gtatgacacg attttaagta tccatctttc ttcagggatt tctggctttg
    68881 tcaacacatt atttgcgatg aaagatgaca tcagcggagt aacggtcatt ccttatgact
    68941 ctaagatcac tagtatgccg atgggacaca tggtagaagc tgccttagac ttaaatgatg
    69001 aaggtcagtc ccttgatgaa atacttgcac atatcgatcg aatccgagat aatacctacg
    69061 cttatctgat tgtagatgat ttgaacaatt tagtacgagg aggacgtttg acaaatggtg
    69121 cagcgctgat tggcagctta ttaaaaatca aaccgatcct gacattttct gaagggaaaa
    69181 ttgttttatt cgaaaaaatc cgttcaagta aaaaagcatt tgctcgagct gaagaaatta
    69241 ttggtagaag aaataacgaa atacaaccgc cagtcaaact ttacgtgata catgcaaata
    69301 atttagaagt agcgattgcg gagaaagaga aattgcagaa aaaatatcca aatgcagaaa
    69361 tcgaaattgg tcatttcgga ccagtcattg gtacacattt aggtgaaaaa gccatcggca
    69421 ttgccatttc agcgcaataa ttccttttat tcaaaaaaca taccaaaaat attccaacat
    69481 catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg gtgtttttat
    69541 ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat aatgtcgcat
    69601 atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa aaggatgagg
    69661 agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg ttgtagaaga
    69721 ctatgataaa gttgatggaa gaaatgcatt aaatacggat gcaaaagggc tttcactggg
    69781 ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat ggcgacatac
    69841 tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt tagatttagc
    69901 gatcttagct gcacaaggaa gtttatactt ccaagatgct tatcgttatg aaaaatttta
    69961 tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa tgacggtaga
    70021 agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg atacactaca
    70081 aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt tagatgagct
    70141 aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa tttctcacta
    70201 acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa accgaaaagc
    70261 catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa ttggcaagat
    70321 cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg gaagacacaa
    70381 ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac ctgttttgca
    70441 gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc taataaggaa
    70501 caagaccaag aattactaga agctatttac caaacagata cacttgaagc tgtactgaca
    70561 ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt agcagagcta
    70621 gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta cccttctgaa
    70681 atcgttcgag cgattgaaaa aggatattta ttagagattc aagagccgac tgtcatccga
    70741 gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat gttgaattta
    70801 ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac aaatcgaaat
    70861 taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca tgcggaaaaa
    70921 atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac tatgatccaa
    70981 aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga aactgcaaaa
    71041 gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc aaatacctat
    71101 aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa attatccact
    71161 gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt gctactggaa
    71221 cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca tacaaaagga
    71281 agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc tgaagaaatt
    71341 ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg agaagagaaa
    71401 caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga acgcagtagc
    71461 gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca agaaatgtct
    71521 gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa agaagcgcga
    71581 caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg accagctttc
    71641 caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat agtagaatca
    71701 cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta ccaaaaagga
    71761 aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt gagaaatttt
    71821 gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg gatcgatgaa
    71881 tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc gattgccatt
    71941 gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac agcagaccgt
    72001 tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg atttcaatgg
    72061 ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg tgctgcactt
    72121 cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt gcttaatatt
    72181 agtgacggtc agccaaaagc gttgccggat tatacaggca aaaaagcaaa agaagatatc
    72241 cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc agctattggc
    72301 caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga tattactgac
    72361 ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg ataaaataaa
    72421 gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac cggtaacaag
    72481 gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag tgaaacgata
    72541 tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat cctgctagtt
    72601 tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa aaacttaatt
    72661 atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa tctccatttg
    72721 tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc ggaacgatgg
    72781 aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg attatggatc
    72841 ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa aaatctaaag
    72901 aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa gaaccgaata
    72961 attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact ggacagtatt
    73021 atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag aaattgaaag
    73081 aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga tttcgtttag
    73141 atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag aaccctttct
    73201 caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa catgtttttg
    73261 aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa gaaggaccac
    73321 aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat atccatatct
    73381 ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacttgca ttaagcgctt
    73441 ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac catgatgttc
    73501 ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg tctgcaaaag
    73561 caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa ggacaggaaa
    73621 tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta gattcgaaaa
    73681 gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg gatatcattc
    73741 gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa caatatgctg
    73801 gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg atcaatgtag
    73861 aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg atccgcatta
    73921 gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat gaacatccac
    73981 aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg gtaaacttaa
    74041 cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg acattactct
    74101 tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct tatgaagctc
    74161 ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg gactttgcga
    74221 ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct aggaccaccg
    74281 atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt tttcaaacga
    74341 ggtctgacag gtacttggac atgtttgata tgcataccga ttgatgtgtc accgatatcg
    74401 ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg gtcaaaagca
    74461 gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat ttcgaaattt
    74521 tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc acaaccttgg
    74581 acagctagcg caatttcttt aggatccaac aactctttta acgtacggat gatccattga
    74641 ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct tgtgctgcat
    74701 cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc attaactatt
    74761 tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa ccttcttttt
    74821 caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag gaaaggcaag
    74881 tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc cagccggcca
    74941 atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc ttcctaataa
    75001 aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa catatccttg
    75061 taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa tcaaatcgat
    75121 caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa gaaaagcagc
    75181 ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta cagggatcaa
    75241 aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg ttaattgctt
    75301 agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt ccggtatgat
    75361 cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt tctgctactg
    75421 ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa aaggcacaac
    75481 cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca taatgatcaa
    75541 ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct ttgatcacaa
    75601 tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct gttaacgaag
    75661 agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta atcatcgtaa
    75721 caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc acagcttcag
    75781 tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt gtttttatga
    75841 agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg ataccatcta
    75901 atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa gatatagatt
    75961 ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg cttagtccga
    76021 atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca gcaaatggat
    76081 cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat aagagtataa
    76141 cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc caatttaaaa
    76201 aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt taggactttt
    76261 gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct gttcgcggga
    76321 ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg acttgctccc
    76381 accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt tgtggagaat
    76441 gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct aataaaaagt
    76501 cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga tctttcaaaa
    76561 ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa tcgccataaa
    76621 cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct ttagacagta
    76681 attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct gtaatccgag
    76741 cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt ttcaaactct
    76801 ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat acttgaagaa
    76861 ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg ttgtttatag
    76921 catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc tccttagcag
    76981 taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc cggtgagctt
    77041 ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata ggaaatccca
    77101 tggacatcaa agaagagata ccaatattga atctggaatg cactggagag atgtgaaaag
    77161 cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc gtccgccatc
    77221 ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca gaagtatggg
    77281 tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa gcaggttgtt
    77341 ccattaaata aagaatagg
//
LOCUS       DACCCS010000005        72923 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.005, whole genome shotgun sequence.
ACCESSION   DACCCS010000005 DACCCS010000000
VERSION     DACCCS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.005"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(161..445)
                     /locus_tag="IVK82_000337"
     CDS             complement(161..445)
                     /locus_tag="IVK82_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391425.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(463..1353)
                     /locus_tag="IVK82_000338"
     CDS             complement(463..1353)
                     /locus_tag="IVK82_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP6391426.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1373..1591)
                     /locus_tag="IVK82_000339"
     CDS             complement(1373..1591)
                     /locus_tag="IVK82_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391427.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1561..3261)
                     /locus_tag="IVK82_000340"
     CDS             complement(1561..3261)
                     /locus_tag="IVK82_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP6391428.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3528..4301
                     /locus_tag="IVK82_000341"
     CDS             3528..4301
                     /locus_tag="IVK82_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391429.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="IVK82_000342"
     CDS             complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="IVK82_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP6391430.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5017..5119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5125..6096)
                     /locus_tag="IVK82_000343"
     CDS             complement(5125..6096)
                     /locus_tag="IVK82_000343"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP6391431.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="IVK82_000344"
     CDS             complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="IVK82_000344"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP6391432.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8066..8884)
                     /gene="purR"
                     /locus_tag="IVK82_000345"
     CDS             complement(8066..8884)
                     /gene="purR"
                     /locus_tag="IVK82_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP6391433.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9051..9875)
                     /locus_tag="IVK82_000346"
     CDS             complement(9051..9875)
                     /locus_tag="IVK82_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP6391434.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9848..10528)
                     /locus_tag="IVK82_000347"
     CDS             complement(9848..10528)
                     /locus_tag="IVK82_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP6391435.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10551..11489)
                     /locus_tag="IVK82_000348"
     CDS             complement(10551..11489)
                     /locus_tag="IVK82_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP6391436.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11734..12585)
                     /locus_tag="IVK82_000349"
     CDS             complement(11734..12585)
                     /locus_tag="IVK82_000349"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP6391437.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="IVK82_000350"
     CDS             complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="IVK82_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP6391438.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15453..16259
                     /gene="proC"
                     /locus_tag="IVK82_000351"
     CDS             15453..16259
                     /gene="proC"
                     /locus_tag="IVK82_000351"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP6391439.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16338..17915)
                     /gene="rny"
                     /locus_tag="IVK82_000352"
     CDS             complement(16338..17915)
                     /gene="rny"
                     /locus_tag="IVK82_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP6391440.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQT
                     LNRKDDSLEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDA
                     RDVIMKSTEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSV
                     VTLPNDEMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLE
                     KLIQDGRIHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFR
                     TSYGQNVLKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGA
                     ELAAKYKENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRL
                     RSLENISNSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPG
                     HIKVIVIRETRATDYAK"
     gene            complement(18327..19376)
                     /gene="recA"
                     /locus_tag="IVK82_000353"
     CDS             complement(18327..19376)
                     /gene="recA"
                     /locus_tag="IVK82_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP6391441.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19485..20726)
                     /locus_tag="IVK82_000354"
     CDS             complement(19485..20726)
                     /locus_tag="IVK82_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP6391442.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20966..21544)
                     /gene="pgsA"
                     /locus_tag="IVK82_000355"
     CDS             complement(20966..21544)
                     /gene="pgsA"
                     /locus_tag="IVK82_000355"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP6391443.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21753..22637)
                     /locus_tag="IVK82_000356"
     CDS             complement(21753..22637)
                     /locus_tag="IVK82_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6391444.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(23415..24701)
                     /locus_tag="IVK82_000357"
     CDS             complement(23415..24701)
                     /locus_tag="IVK82_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP6391445.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24694..25962)
                     /locus_tag="IVK82_000358"
     CDS             complement(24694..25962)
                     /locus_tag="IVK82_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP6391446.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26214..26675
                     /gene="mscL"
                     /locus_tag="IVK82_000359"
     CDS             26214..26675
                     /gene="mscL"
                     /locus_tag="IVK82_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP6391447.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26734..27180)
                     /locus_tag="IVK82_000360"
     CDS             complement(26734..27180)
                     /locus_tag="IVK82_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6391448.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(27273..27518)
                     /locus_tag="IVK82_000361"
     CDS             complement(27273..27518)
                     /locus_tag="IVK82_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391449.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(27633..28211)
                     /locus_tag="IVK82_000362"
     CDS             complement(27633..28211)
                     /locus_tag="IVK82_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6391450.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(28288..28818)
                     /locus_tag="IVK82_000363"
     CDS             complement(28288..28818)
                     /locus_tag="IVK82_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP6391451.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(28822..29682)
                     /gene="rlmB"
                     /locus_tag="IVK82_000364"
     CDS             complement(28822..29682)
                     /gene="rlmB"
                     /locus_tag="IVK82_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP6391452.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(29675..30070)
                     /locus_tag="IVK82_000365"
     CDS             complement(29675..30070)
                     /locus_tag="IVK82_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP6391453.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(30067..31479)
                     /locus_tag="IVK82_000366"
     CDS             complement(30067..31479)
                     /locus_tag="IVK82_000366"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP6391454.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(31476..32012)
                     /gene="cysE"
                     /locus_tag="IVK82_000367"
     CDS             complement(31476..32012)
                     /gene="cysE"
                     /locus_tag="IVK82_000367"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP6391455.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(32334..33794)
                     /locus_tag="IVK82_000368"
     CDS             complement(32334..33794)
                     /locus_tag="IVK82_000368"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP6391456.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(33964..35121)
                     /locus_tag="IVK82_000369"
     CDS             complement(33964..35121)
                     /locus_tag="IVK82_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP6391457.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(35155..36525)
                     /gene="radA"
                     /locus_tag="IVK82_000370"
     CDS             complement(35155..36525)
                     /gene="radA"
                     /locus_tag="IVK82_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP6391458.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(36588..37115)
                     /locus_tag="IVK82_000371"
     CDS             complement(36588..37115)
                     /locus_tag="IVK82_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP6391459.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            37345..38151
                     /gene="proB"
                     /locus_tag="IVK82_000372"
     CDS             37345..38151
                     /gene="proB"
                     /locus_tag="IVK82_000372"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP6391460.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            38148..39395
                     /locus_tag="IVK82_000373"
     CDS             38148..39395
                     /locus_tag="IVK82_000373"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP6391461.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            39471..39815
                     /locus_tag="IVK82_000374"
     CDS             39471..39815
                     /locus_tag="IVK82_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391462.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(39932..40258)
                     /locus_tag="IVK82_000375"
     CDS             complement(39932..40258)
                     /locus_tag="IVK82_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391463.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(40631..41832)
                     /locus_tag="IVK82_000376"
                     /pseudo
     CDS             complement(40631..41832)
                     /locus_tag="IVK82_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            41949..42464
                     /locus_tag="IVK82_000377"
     CDS             41949..42464
                     /locus_tag="IVK82_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6391464.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(42663..43109)
                     /locus_tag="IVK82_000378"
     CDS             complement(42663..43109)
                     /locus_tag="IVK82_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP6391465.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(43156..44634)
                     /gene="msr"
                     /locus_tag="IVK82_000379"
     CDS             complement(43156..44634)
                     /gene="msr"
                     /locus_tag="IVK82_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP6391466.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(44988..45617)
                     /locus_tag="IVK82_000380"
     CDS             complement(44988..45617)
                     /locus_tag="IVK82_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP6391467.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(45601..46065)
                     /locus_tag="IVK82_000381"
     CDS             complement(45601..46065)
                     /locus_tag="IVK82_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6391468.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(46552..47820)
                     /locus_tag="IVK82_000382"
     CDS             complement(46552..47820)
                     /locus_tag="IVK82_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP6391469.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(48051..48788)
                     /locus_tag="IVK82_000383"
     CDS             complement(48051..48788)
                     /locus_tag="IVK82_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6391470.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(48855..49424)
                     /locus_tag="IVK82_000384"
     CDS             complement(48855..49424)
                     /locus_tag="IVK82_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP6391471.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            49552..50424
                     /locus_tag="IVK82_000385"
     CDS             49552..50424
                     /locus_tag="IVK82_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6391472.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            50766..51638
                     /locus_tag="IVK82_000386"
     CDS             50766..51638
                     /locus_tag="IVK82_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6391473.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            51635..53593
                     /locus_tag="IVK82_000387"
     CDS             51635..53593
                     /locus_tag="IVK82_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6391474.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(53844..54695)
                     /locus_tag="IVK82_000388"
     CDS             complement(53844..54695)
                     /locus_tag="IVK82_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6391475.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            54818..55285
                     /locus_tag="IVK82_000389"
     CDS             54818..55285
                     /locus_tag="IVK82_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP6391476.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(55548..56030)
                     /locus_tag="IVK82_000390"
     CDS             complement(55548..56030)
                     /locus_tag="IVK82_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6391477.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(56130..56993)
                     /locus_tag="IVK82_000391"
     CDS             complement(56130..56993)
                     /locus_tag="IVK82_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6391478.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            57591..58439
                     /locus_tag="IVK82_000392"
     CDS             57591..58439
                     /locus_tag="IVK82_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391479.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(58610..58951)
                     /locus_tag="IVK82_000393"
     CDS             complement(58610..58951)
                     /locus_tag="IVK82_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP6391480.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            59037..59744
                     /locus_tag="IVK82_000394"
     CDS             59037..59744
                     /locus_tag="IVK82_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP6391481.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            59731..60054
                     /locus_tag="IVK82_000395"
     CDS             59731..60054
                     /locus_tag="IVK82_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP6391482.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(60143..61180)
                     /locus_tag="IVK82_000396"
     CDS             complement(60143..61180)
                     /locus_tag="IVK82_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6391483.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(61627..62610)
                     /locus_tag="IVK82_000397"
     CDS             complement(61627..62610)
                     /locus_tag="IVK82_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP6391484.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(63026..63796)
                     /locus_tag="IVK82_000398"
     CDS             complement(63026..63796)
                     /locus_tag="IVK82_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6391485.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(63789..64724)
                     /locus_tag="IVK82_000399"
     CDS             complement(63789..64724)
                     /locus_tag="IVK82_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6391486.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(65318..66967)
                     /locus_tag="IVK82_000400"
     CDS             complement(65318..66967)
                     /locus_tag="IVK82_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP6391487.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(67162..68058)
                     /locus_tag="IVK82_000401"
     CDS             complement(67162..68058)
                     /locus_tag="IVK82_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6391488.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(68051..68731)
                     /locus_tag="IVK82_000402"
     CDS             complement(68051..68731)
                     /locus_tag="IVK82_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6391489.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(68732..69514)
                     /locus_tag="IVK82_000403"
     CDS             complement(68732..69514)
                     /locus_tag="IVK82_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6391490.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(69507..70424)
                     /locus_tag="IVK82_000404"
     CDS             complement(69507..70424)
                     /locus_tag="IVK82_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6391491.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(70427..70711)
                     /locus_tag="IVK82_000405"
     CDS             complement(70427..70711)
                     /locus_tag="IVK82_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6391492.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(71412..72032)
                     /locus_tag="IVK82_000406"
     CDS             complement(71412..72032)
                     /locus_tag="IVK82_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391493.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(72113..72523)
                     /locus_tag="IVK82_000407"
     CDS             complement(72113..72523)
                     /locus_tag="IVK82_000407"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391494.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            72553..>72923
                     /locus_tag="IVK82_000408"
     CDS             72553..>72923
                     /locus_tag="IVK82_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6391495.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDILEE
                     TVIHLFRKGITMSEIADLIEK"
BASE COUNT        20984 a        15542 c        12855 g        23542 t
ORIGIN      
        1 taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag attgtgacac
       61 gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg aagaaatcca
      121 attctgatta tttattgtat ttctttgttc agcatccttg ctaaaagccc atattggaca
      181 aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct gtcggatctt
      241 ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg taagcaccgt
      301 cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct ttccatacgt
      361 caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca cttaattcgt
      421 ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa tattttgtac
      481 ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt taatttcgat
      541 tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga tcaggaaatc
      601 taattcccga cctacaggac attgtgcttc gataagttca tctagttttt gtacatggat
      661 cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt
      721 caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt tttgatacct
      781 tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta gtacttcttt
      841 gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt tttctcgact
      901 cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg ccaattcttc
      961 catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagcg cgaagcgttc
     1021 cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa ttccgtctgt
     1081 caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt tttcgttttt
     1141 gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact gctgtttaat
     1201 aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta tttgaacatc
     1261 tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt ttgtttcgcg
     1321 gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct cattacatat
     1381 tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg cctttcccca
     1441 ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga ccattccagc
     1501 tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata gcgatttctt
     1561 ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat aatataaata
     1621 taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac gatccttgcg
     1681 ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt gtattttttg
     1741 ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt gtgccaataa
     1801 atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca tgtctacttg
     1861 atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca tcgccaatcc
     1921 gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc aaaattttat
     1981 gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg
     2041 ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat agtaatagcc
     2101 taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc tttggctatc
     2161 aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc
     2221 tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa atgcctctgt
     2281 tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac cgtctgcata
     2341 tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag aggcgagtat
     2401 ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt ccgctagttc
     2461 tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga gtaaggacaa
     2521 atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc cttcaattag
     2581 tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt cttcaactgc
     2641 agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata aattcacagc
     2701 ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg aggtacctgt
     2761 gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa tctgataagc
     2821 accgtgcgct cctcctccac ctaaaacaag tgctgtatga tagaccagct ttttatttaa
     2881 ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt ctgatagagg
     2941 agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag cttccatata
     3001 ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat taaaaataac
     3061 atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata ctgctttaaa
     3121 agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat gactttgaaa
     3181 aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt tctgtgaaaa
     3241 gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca gtacgtcata
     3301 aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa ttcataaaac
     3361 ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag agtgttttca
     3421 atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt tttaatgcaa
     3481 atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg actgagtcag
     3541 tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg actgctactg
     3601 actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat ccatctgctt
     3661 ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca gaaaatattc
     3721 agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag tggatcgact
     3781 ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa ttattatatt
     3841 ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag aataattttg
     3901 tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta gatgaatttt
     3961 ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat
     4021 cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc
     4081 gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg atcgatggta
     4141 aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa ggcacccggt
     4201 atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga cactggcaat
     4261 tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc ttatcaaaaa
     4321 ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc ttctttttta
     4381 tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt tggatcccta
     4441 ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc attacgaaag
     4501 aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc actcctgcaa
     4561 taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg atgctttttc
     4621 taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc atgagccctg
     4681 ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg acacttaaat
     4741 aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc agcatccctg
     4801 cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa
     4861 aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct gcaacaatcg
     4921 ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg aataaaaaag
     4981 tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca caattcctac
     5041 gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc agcccaattg
     5101 gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg gactaactgg
     5161 tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac ttacttcatc
     5221 gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa ctagacgctc
     5281 gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat gtgtacatga
     5341 agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg tgatcgtacc
     5401 tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt gtccaatgat
     5461 attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg caatcggaga
     5521 tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac
     5581 gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa gtggcgcacc
     5641 catcaaatga tcgactggaa tatcaaagaa accttggatt tggactgcat gaaggtccaa
     5701 agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt tcgctgtgat
     5761 tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag gcatgactac
     5821 gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca actccatcaa
     5881 gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc cacgaatact
     5941 ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg attttcctaa
     6001 ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat ttaacgcaaa
     6061 aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt tctttttcac
     6121 aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc aagtgatttt
     6181 gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat tgacttgtct
     6241 tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg acccggcagc
     6301 aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag aaccaataaa
     6361 gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga cgaagacaac
     6421 accacatccg acattgattt cttctcccag cgtagcatca ccaacataag tcaagtgacc
     6481 gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc cgatatggac
     6541 gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt
     6601 cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct caatcctact
     6661 gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg tatttccttg
     6721 gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg catcgatgta
     6781 tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt taattcgctt
     6841 tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca ttgattcttc
     6901 aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga tttcgatgat
     6961 atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta acgcatcgaa
     7021 caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa gagcttcttc
     7081 tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt cacggatgat
     7141 tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac ttgcattttt
     7201 tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg gtgtatcgcc
     7261 actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc
     7321 atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc
     7381 tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt tcgtttcact
     7441 gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta aaactttata
     7501 taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat aacgtgcgtc
     7561 caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta tacccatttt
     7621 ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa aatagataga
     7681 ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt
     7741 cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt
     7801 aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc ttcggtatta
     7861 gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta atactcagga
     7921 caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt tctcggtatt
     7981 aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
     8041 aactaagcag ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa
     8101 tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg
     8161 cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga
     8221 attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca
     8281 ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc
     8341 tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt
     8401 cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta
     8461 cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa
     8521 tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat
     8581 atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt
     8641 ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga
     8701 ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt
     8761 cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt
     8821 catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt
     8881 tcacctattc caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc
     8941 aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa
     9001 gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt
     9061 tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata
     9121 aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct
     9181 gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg
     9241 cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg
     9301 gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc
     9361 aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc
     9421 ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg
     9481 acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct
     9541 gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa
     9601 atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca
     9661 gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct
     9721 aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg
     9781 atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac
     9841 gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc
     9901 ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg
     9961 atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt
    10021 catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac
    10081 gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca
    10141 tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga
    10201 gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac
    10261 taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt
    10321 ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta
    10381 acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt
    10441 cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg
    10501 tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata
    10561 ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg
    10621 atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca
    10681 ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg
    10741 ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct
    10801 ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa
    10861 gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt
    10921 tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt
    10981 ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat
    11041 accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat
    11101 tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc
    11161 atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct
    11221 gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt
    11281 tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct
    11341 acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct
    11401 tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt
    11461 accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa
    11521 ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat
    11581 ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc
    11641 tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt taggattcct
    11701 cagtttttca tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac
    11761 ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca
    11821 caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat
    11881 catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact
    11941 gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc
    12001 gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga
    12061 gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg
    12121 gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc
    12181 tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa
    12241 ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc
    12301 tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt
    12361 tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt
    12421 tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc
    12481 tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc
    12541 cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg
    12601 aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc
    12661 ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca
    12721 aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt
    12781 tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc
    12841 tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata
    12901 agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc
    12961 ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc
    13021 ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa
    13081 ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat
    13141 cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct
    13201 agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg
    13261 aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta
    13321 ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg
    13381 agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaagtgaaa
    13441 acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg caagctgata
    13501 gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt
    13561 gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac
    13621 ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg
    13681 gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc
    13741 actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa
    13801 tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa
    13861 atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta
    13921 aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac
    13981 gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata
    14041 taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag
    14101 taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc
    14161 gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg
    14221 caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac
    14281 catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa
    14341 aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa
    14401 caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca
    14461 aactactgct acatcttgcc gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc
    14521 aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa
    14581 cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa
    14641 taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt
    14701 tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg
    14761 tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg
    14821 aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa
    14881 tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa
    14941 actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt
    15001 tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc
    15061 taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc
    15121 cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg
    15181 ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc
    15241 gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg
    15301 tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac cccaatgtta
    15361 tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag
    15421 aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga
    15481 atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg
    15541 tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc
    15601 atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga
    15661 aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat
    15721 tcatttctat tgcttcagga aaatcattaa cagatttaga aacagcgctt gaagatcctc
    15781 aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg
    15841 cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg atgatttttg
    15901 aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg
    15961 ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac
    16021 tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt
    16081 gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc
    16141 ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa
    16201 tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaataat
    16261 gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt gccgcggctc
    16321 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
    16381 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
    16441 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
    16501 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
    16561 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
    16621 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
    16681 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
    16741 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
    16801 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
    16861 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
    16921 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
    16981 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
    17041 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
    17101 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
    17161 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
    17221 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
    17281 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
    17341 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
    17401 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
    17461 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
    17521 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
    17581 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
    17641 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
    17701 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
    17761 agagtttctg cttctttttt tgcactatcg atgattccag cagctgatga ttgtgcacca
    17821 gctatttctt tctcgtgacg tgactttgcg accataaacc ctaaaccaag accgacaatt
    17881 aaaccgatga tagcgaggag aatgttgaat accataaaat ccacctccat actatctttt
    17941 tcatttttat catctataca gcttctatga taaattattc taaactcgtc aatatgcatc
    18001 ttcgcatata tgtgtacaaa aaacatgcac aactttattc taatgtttgt accccgtagt
    18061 gtcaacttta aataaacaaa gaaacggctt tctacttatt cattaaacga tggtttttaa
    18121 aataaatgac actagtttca tataggtaaa cctttaaatt gagaaacttt tctattaaat
    18181 tattctttgt agatgaacag atatttttat tttatgtacc cactcgtata attaaataac
    18241 tcacgatttc tttattcaat aaaaaaaacg gcaggtcaag gagagtacct actcttcaga
    18301 ccagccgttt ttgtttatca taatttttat tcttctacta aaggcaattc ttcttgtcct
    18361 ttttcatctt ctgggacagc gacttcttca ccaataccat aagctgctct aactagagca
    18421 gaaacttctg ccatcatttc aggatgatta gccatataga ttttagcgtt ttcccgtcct
    18481 tgaccaatcc gatcttcttt ataagaatac caagcaccgc ttttatcaac aatgtctttt
    18541 tctactgcca tgtctagaag ttctccttct tgtgagatcc ctaaaccata catgacatct
    18601 acttcagcga ttttgaaagg aggtgctact ttgtttttta cgactttgat ctttgtacgg
    18661 ttaccaacaa tatctgtacc ttgtttcaat tgttctgcac gacgtacttc caaacggatc
    18721 gttgcgtaga atttcaatgc gcgtcctcca ggagtgattt ctggattccc aaacatcacg
    18781 cctacttttt cacggatttg gttgatgaaa atagcgattg tttttgtttt gttgatcgaa
    18841 ccagagagtt tacgcaatgc ttgagacatc aaacgtgctt gtaacccgac atgtgagtca
    18901 cccatttcgc cgtcgatttc tgctcgtgga actaaagcag caactgaatc gactactacg
    18961 atatctacag ccccacttga tactaaagca tcagcgatct ctagaccttg ttctcctgtg
    19021 tcaggctgtg aaagaagtag ttcatcgata ttcacaccta atttttgtgc atattgcgga
    19081 tctaacgcat gctcagcatc aatgaaagcg gccgttccgc catttttttg tacttctgca
    19141 atagcgtgta gtgcaactgt tgttttacct gaactttcag gtccatatac ttcgatgata
    19201 cgtccacggg ggtaaccgcc gacacctaaa gcgacatcta acgcaagaga gccacttgga
    19261 attgtagaga tttgttggtc gattttttcg cccaatttca taatagagcc tttaccatag
    19321 ctcttttcaa tttttttcag tgcagcatct aaggctgctt tacgatcatc tgccaatacg
    19381 ataatcctcc ttatatttac aaatcagagc aacatttcat tatccttatc atatcttttt
    19441 tagtgaaaaa aagcaagaaa aaaccgaaca aatattcgca tttatcattt tcttaaaagc
    19501 tgttttctca aaagatcaaa tccttgcata accgcattat atctgatagc agcacgatct
    19561 ctggcaaagt gatatttttc cgaaaccacg ccgtctttag atgaaatagc gatccatact
    19621 gttcctgcag attgaccttc taacgtgtcc ggtccagcca ctccggtgaa agctacactg
    19681 taatctgttc ttgctatctc acgtgcttga atggccatct gctccacaca ttctttgctg
    19741 actgttccat attctgctaa tagctccgga tcaatctcca gaaaaccagc ttttgtttgg
    19801 agcgagtagg taacgaatcc gcctggaaat acttcagata caccagcaat gtcgcctaat
    19861 gcggcttgga aggcgccggc tgtcaaactt tccgctgctg ttacagtctg cttgttttct
    19921 ttcagtagtt ccactacgac ttttgctagc gaattatcat cgccgtatcc ataaaaatat
    19981 tcccctactc gttcttggat tttttcttct aatgctaaaa gagcttgatt ccctgcctga
    20041 acatcatttg ttttcactgt cagtcgtaat gtcacttcat ttggtttggc atatggtgcg
    20101 atcgttggat tgatctgtgt ctcaatcaga tcttttagct cagtgaccag tcgcgattca
    20161 ccaatcccat aaaagcgcaa aactttagaa attagttttt cttcagaagg aaaacgttcc
    20221 tctagtaaag gacgtacttg ttccacaaac attggtttca actcattagg cggaccggga
    20281 agaaggatat acgcatgtgt atctgtttga taaaaagtcc ctaaagcaag ccctgtacga
    20341 tttggtagag gaaccccgcc ttcgataatc tgggactgtt gcagattgtt tttggtcatt
    20401 tttcgatggg ttgtttcaaa atatgccaat aattttttgt atccttccgt attttgtatg
    20461 agagacttac cgaggtgagc cgcagttgct tcttttgtca ggtcatcttc tgtcggtcct
    20521 aatccgccac ataagagtat gagttcactt cttgtttcag ctagagcaat caactcttct
    20581 aaacgctgct gattatctcc tacaactgtt tgataataaa cttcgatacc tagatcagct
    20641 agctgttctg acaagaaagt ggcattcgta ttcacgactt gtcctagtaa aagttctgtt
    20701 ccgacagcta ttatttctgc tttcattact tcccgctccc ttatatagta tacgctgtat
    20761 ttttatattt ttttatttaa aaagagggca aggagctgtg acaaaaatct tgtcacagct
    20821 ccttgttcca aataaacagt gttctcctgc caaaacctcg gtcataagtc acaatataca
    20881 taaaacatga aaagctgttt tttgaaatta tcccttgtga ctcagagtta gacggcaggc
    20941 tcacaacctc tccactaaac gatacttaca ttgatccttt gaacacaccg ctgtttttga
    21001 tgaagtaatc cacacctgaa tagatagtaa agatcagaca agcataaagc atgatttgat
    21061 ctactggaat accgatcagg ctgaatggga tattattgat aaacagcaag ataatagcaa
    21121 ccatttgtgt agccgtcttg acttttcccg gccaagctgc agccatgact tctccatgtt
    21181 cgacaagtag taaacgcaag cctgtgacgg ctaattcacg acagacaatg atcgctacta
    21241 cccaaccagg ggcctttcct tggtcaacca acatgataaa agcagtcata actagcattt
    21301 tatctgccaa cgggtccgca aacttgccaa aattagtgac aaggcctcgc gcacgggcaa
    21361 tttttccatc taaccagtct gtgatactag ctaaagcaaa aataactgca cctactagct
    21421 gtgtgacagc cagtgtggtg tctcctacgt taaattgtcc ccaatctaaa ggtaaaccta
    21481 caaccaaaat gaaaatcggg atcatacaaa ttctaagtac agttaattta tttggtaaat
    21541 tcacactatc gactcctttc ttccctattc tacgtgacta tcagcgaagt ttccaccgat
    21601 attatttact tttccaaatg aatttattca aaggagtatt tttattgaaa ttgatctttt
    21661 tgaattaacg aagaaggacc gcgacacaga tttgtcacga tccttctttt ttatacctac
    21721 aagtaaacag atgcgtaaac taatttatac gatcattctg cgtattgaag agtaaggttc
    21781 acattttttg gactctgatt gttttcccct ggattaactg gtacggattc tccattcgct
    21841 ttgatcgttg cattgcttgc tgcaccaatc gtgatggtcg catttgttgc tgtttccggt
    21901 aacacggtac tctgtgtttc atttgctgcc agtgtccctt gataaacata ggcattgtct
    21961 actagcacac ctaaccatac acgattgaca gcagtaaatt caagggttac aggcttggta
    22021 gcgtctgtga tatttactgc gatggcactg ccagaatctt ctcctgtagc tattgccatt
    22081 ttcttttctt cacttgattt tgttgttgat tctactgtag aagactcttt ggtctgcgtg
    22141 ctttgctctg aggaagagga ctcctcgact gatccttcta ctttaagcga agaagctgtg
    22201 tctccgatta ttggatcagc ttgacgatct tgccaagtca tatagcccac gacaacgaca
    22261 atcgccaaag caaccaatcc taataaaatg acaggaagac ttgtccaaaa gcgacttgga
    22321 tgcgattctt cctgatgcat tgcttttcta gaagtactta ctgtttcagg ctgcggacgt
    22381 tcagggaccg atcctgtcaa tttgcttttc ccatcaaaaa catcgactaa atggtctcca
    22441 tcttctccta cagcttcagc gtattgccga ataaatgccc gcacgtaaaa tttaccaggc
    22501 aattggtcaa aatcatcatt gtcgattgct tccaaataac gtttttgaat ttttgtaatc
    22561 tgctgtaatt catctaaaga tattttttta ttcaggcgtg cttgccgtaa tttttctccg
    22621 atggtttcaa aagccactcg ttcatcttct ccagtctgtt tgattctccg aagcatctgc
    22681 tgttgcaacg aaagacgtta tgtttgggtg ctgtgacaaa agtcatgtca cagctccttt
    22741 tttccgaata aacattgttc tcctgccaac tcctcggtca caagtcacaa tatccaataa
    22801 atataagaag ctgttttttg aacttgttcc ttgtgactcg gagttaaacg gcaggctcac
    22861 gacctctttc ttaaacggtg ttcaatcagc tgttcctcgg tattagctca aaaaccggca
    22921 aaatataagg agatattttt ccgtatgttt ctctaatact caggtcaaaa cgctgatttc
    22981 acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag tccaaccaag
    23041 ttaaaaacat taagaagctg tttttacttg gctgttctta ttacttgatg cagggcagct
    23101 ttttcacaac ctcttggtac acggtgttcc agaagtagct tcttcggcaa taagccgaaa
    23161 tatcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca
    23221 cttctgtcac aacctctttg attacttcgt ttgattttat tttaatataa gttgattaaa
    23281 agagagcatg atttgtttta ttttctcact aatttttcca acacaactaa gaataccata
    23341 aaagctgatt ttggaggagg ttcctactac aagtctaaaa cgaaatttaa gacttttcct
    23401 cataaaatac tggtttattt tggtctcatg taaaactcgc tgaaggcctc ttcacttata
    23461 aatgcttccc cagctgccag tacgtctttc atttggatcg aatcgatgat ttcaggaaga
    23521 tcaaataaag ttcgatcgcc aaataaactt tgcgtaaatt gattggcaat atattcgatc
    23581 gaatttaatg attggaaata ttgtccaagc attttcttct tcagcaaatc gagatttgtt
    23641 tctgacactt ccggatcatc agcgaatcca agaatgattt ctttgacttt ttctgctgct
    23701 ttctctggtt cgtccgtatc tccgctgaag tccgcaaagt gaaattcacg atccaagctg
    23761 aattcaaaac caaagctgtc atcgataatt ccttgattgt acatcgctag atagtttcga
    23821 gaagtattgc ctaaaatcat ttggaacaac agattgattg ctgttttata gcgtaacagt
    23881 tctgttcctt cttgcggcaa tgtgtctaat cctttaatac caagtacaaa tttatctcgt
    23941 gtaatggctg cttccaatgc actttgtttg atgatttctt ttgtgttttc agggaaataa
    24001 cgtacaattt cttgctttgg agggaaattt tttgcttctt ggttttcccg aataagtttc
    24061 atcaatttct caggctccat cttaccaact acaaataaaa ccatattgct tggctgataa
    24121 aacgtccgat agcacgtata caagtcttga gctgtgattt tgtcgatact ttcaaccgtg
    24181 cccgcaatat cgatatgcaa tgggtgtgta ggatacaagt tatttagtat gccaaaaaac
    24241 atccgccagt tagggtcgtc ttcatacatt tggatttcct gaccgatgat tcctttttcc
    24301 ttgtttacag tttcttctgt gaagtaaggt gcttgtacaa aatcgatcag agtcgtcaga
    24361 ttcttttcta cctgatccgt cgtagaaaac agatagctgg ttttcgtaaa acttgtgaaa
    24421 gcattggcgg aagctccttg tttcccaaat aattgaaaga catcaccatc ttctttttca
    24481 aacaacttat gttctaaaaa atgggcaatt ccatctggaa ctttcacctt ttctttttca
    24541 ccatagggaa taaattcgtt atcgatcgaa ccataattcg tactgaacaa accatatgtc
    24601 ttgtgatagt catttttagg caacaaatac acggtcaaac cgtttggcag tacttcatga
    24661 taaagtgttt cattgatttg ctcatattct gttttattca tttgcatttc ctcctgctaa
    24721 aaagaagatt gcctgcaatt cgatttgctc agctacttgt tgtatctctt tgatcgttac
    24781 gctctccata cgtttcaacc attcttcatc agataatttt gtttccggca gccagctgtc
    24841 caaaaaagaa gcttcgattg ccgcttgcgg agagtctaga gaaagcagat attggtttct
    24901 cagcattgct tttgtctgag acagttccaa ctctgttatt tcaccatttc gcaaactttc
    24961 taattgctcg tgaatcagat gaagtacttg atttcggttc ttttcatcga taccggtttg
    25021 gacactcatg aatcctcgga acgtatccac gctactagag gcataataag ctagactttc
    25081 tttctctctg acattcatga aaagtttaga gtgaggaaaa ccgccaaata aaccgttaaa
    25141 gaccattagc gcaaaacgtt ctggttcatc gtaatacaca tttgtctgat aagctaggtt
    25201 cagctttgct tgaacgattg gctcacgtac ttgtcgttcc ttcacgatat tcacttgagg
    25261 ttgggtataa aacagatctg gataaactgt ttcccgcgac tcaaatggta attgcccaac
    25321 tgcttcagct gctttgtccg gatcaacatc gccgacaaca aaaatgtcga tttgatcatt
    25381 tgccatcatt tcttgatagt aggacgctag cgaactagca gtgatcttgt ccaaatcttc
    25441 gactacgccc aaccctggga tcttttgttc cgggctgttt tcaaaataaa gttcttgcaa
    25501 ggcataggaa gcaaaagttt gtttatcttc cttcaaactt tccaaatata gacgcagatt
    25561 gttcttttcc aagtcaaacg tcaactgatc aaactgatga tttttaatat ttggataaaa
    25621 taaaacctct tttaaaaaat ctaccgcttg agaaaaaagg tttggatcat tgacatattc
    25681 accattcaca aaactgatcc ctgcgtttag ccaatgtaaa tttccttttt ttctaactga
    25741 taatccaaag cttgcgccat aaagctctgc aagttgcgca cttaactttg tctgatccgg
    25801 ataattcaaa ctatttgttt ccaacatgct gctgagaagt gaacgtttcg tgatgacttc
    25861 ttgttgcaga cgcgcagtaa aacggataaa aatccggatc gttttgtatt gttttgttgg
    25921 taagacatga agattcacgc cttctgccaa ttgatacacc atatttatat gctccttttc
    25981 acagttgatt gcccgcttac tcttttaatg ggtcaacctg ccttttttac ttattcctct
    26041 gcttgaaatt tataagttca ggcaattcgc cgttttttct aaatgatgtt acactttata
    26101 gtatagcata gatgtttttt atcaaaagaa acagacggct aaaagcgtat attgtttttt
    26161 tattttttat ttttcgatat actaaagaca aataaaagaa gggagggtca ttcatgatca
    26221 aagaattcaa agagttcatc atgcgtggag atgtcttgga tcttgctgtc ggcgttgtta
    26281 ttggtagtgc gtttacaagc atcgtaaacc aagtggtgca aggcttgatc acaccactaa
    26341 taggttggtt tgtcgcaatg atcacaggta cttctgattt agaaggagca ctttcaattc
    26401 ttgactggtc gccgacaaaa ggcgtcacat ttgcgtttgg taacgttatt agtgctatca
    26461 ttacatttct gatcactggt tttgtcttat tcttagttgt caaagcggca aaccgtgcga
    26521 agaatttacg ctccaaagaa gagcaagtgg aagtggaaga aactcctact gcagaagatt
    26581 atcttagtga catacgcgat cttcttgcca aacaagtggc agaagacaat acacatccag
    26641 tagagcaaac aaatctttcc gatacttcta aataataaag aatctaaaaa aaagaaagcc
    26701 gattttcagt gtcggctttc tttttttaga ttcttaactt gctacttgtt cctttttcat
    26761 gtcgatatgt ggaatatcat cttccaaata cacatctgag attgccttga atccaaacga
    26821 ctcataaaaa gcttgtaggt attcttgtgc gccgatcacg attggttttt ctggaaaatt
    26881 tttctggatc atgttaatcg tttcttgaac aatctttctt cctaaaccat ctttacggtt
    26941 gtcctgatga acaaggacac gtccaaaatg gatactctcc gcttcttcat atactcgcgt
    27001 gtaggcaagg atcttcccat cagtatcttg aaaaaacgta tgccaagctt gtccatcaat
    27061 gtcatcaact tcttgataag gacactgttg ttccacaaca aaaactgcga ttcgttcttt
    27121 gacgatcgcg taaaactctg ctgttgacaa ttcttcaaat ttctttacct gatattccac
    27181 taagcaaacg ccccctgctt ttttattctt agcataccga ttcgcacaca aaaaagcaaa
    27241 cgaaccttac gaatctcagg ctcgtttgct ttttaaactg cttcagttaa aaattcgatc
    27301 tcaactgtcc gagtaagtac atctgaatag ctgtaagata ctcgttcaaa agaattttct
    27361 tcaggatcca aatcaacaac gaatactgaa ggataggttt ctgttaaaac gccgcggcgt
    27421 tctgtttgac gctttcttcc tgtctgagca accaacatga ttgggctgcc gatacgacct
    27481 tctaaatctt tcttgattga agctaaagtt gttggcatac agttcacctc tactatcaac
    27541 tataccacat atttttaaaa ttttcaagtt taccataaaa aaaaaagaaa tgcaataaaa
    27601 ataaataaga agctcattaa gatttttcta aattattcct cattataaaa tttcaagtgt
    27661 ttttttattt tgagttttac tctcccgtaa gcgctcatta cttgcttgtg atccattgct
    27721 aaacattcag ccatgtctac aaaatcaacg ccttgcatat aataatgaaa aattttagct
    27781 tctatttttg aaaactggat ttcgcaattt tcgattgctt cttttaataa aagatgctga
    27841 atcgctaaag gttcttgcaa tgtgatctct gctaaaaagt ctggtccttc tgtctctatt
    27901 ttttggtcga ttgagacttc atctttttga tcacgacgct tcagcgcgtt ctgcttcctc
    27961 aacagagaat agatttgatt gcttataatg cttttataat aacttccaaa gctaacacct
    28021 ttgctttttt cgtaagttct caatgcctga tataaagcga tccttgcttc ttgttcccaa
    28081 tcctcagaaa cataatttct taaataatag gtgtttttca aacgtagtaa aagtggaaaa
    28141 tatttttgat acagggcagc aaatgcttct tcgtcaccat tacgtgcagc gcgcaataac
    28201 tcctcagaca taaaataatt cctccataaa ttcgtttatg gaggaatctt atcatcaatg
    28261 attcatcgaa ctgattagaa cgatatgtta tttatttttt ctcattaaat cgtccaattt
    28321 ttgggacaat ttttttaact gctcttcatt ccatggagaa ttacgtcgga aatcaataaa
    28381 atgcatatct ttattttctt gggctattgt tttttccgtt ttttttatcg tcttatataa
    28441 ttcataagca gaagttcgta atgctccttg ggaaaagatc atccactgtt ctgctaaatc
    28501 gcttgtcgcc actgtcactt gtgtcaaacg gtcattcagc tgaccagcga ttctctcgat
    28561 ataactgtca gccgtttcgt cttctttcgt aaaaacaaca tccaactggt atttcttata
    28621 attttgttgg atgcccggaa ctagttgggc atcgaagaca acgatgactt ccattccttc
    28681 atatttcgca taatttgata aacgatgtaa aagagcttct cttgcatccg ccattttgtc
    28741 ctgcttttta agctggacaa gttccggcca cgaaccaatc atgttgtagc cgtctacaat
    28801 caacagctgt tttttcatgg tctactcccc ctgacgttgg gaaaaagcct gatacataag
    28861 caaaccagca gcaacactgg catttaagct ctggacgtgc ccggtcatcg ggatcgtcag
    28921 caattcatcc atttcttttt tcaatcctgg actcattcct ttgccttcat taccaatgac
    28981 taaagcaatt gatcctttgg cattccattt ttggaagttt gtgccggtca tatctgtgcc
    29041 aaatacccaa aatcctgttt ctttgagctg ttgcacagct tgtaccaaat tcgtcacgcg
    29101 tgcaacaggg acatgttcga ctgccccagt ggaagtcttt gttacgatag gtgtgattcc
    29161 aactgctcgg tgttttggta tgatcacacc atcgacgcca cttgcgtctg ctgtccgtaa
    29221 aatcgaccca aaattgtgtg gatcttcgat gctatccaaa atcaaaaaga aggggtcttc
    29281 ttttttctct tttgttcgtt caatcagttg gtcaagggat aaatattcat aaggtgtaat
    29341 cgcaagaacg actccttgat gcacgccgtg atcgctcaaa gtttctaatt tttgtttagg
    29401 aacccatttg actggcacag cttgttcacg tgcgatttct ttcagttctt ctactttttc
    29461 tcctcgtgaa tcttcttgaa gaaagagttt attccctcgc ccttgttgta gcgcttctac
    29521 tgcggcatgg tgcccaaaga cgaaattatc ttctaatgct tcttcatttt gatcagaaac
    29581 tgactgattt cgtccagttt gctttttatt tgaatatcta ttttgttttt tgtcttcaaa
    29641 tccacgtttc gattgatgtt tgtttgttct ttctttaccc atttttttca ccaactttct
    29701 gaatacacca gcggatcaat tcttccatcc gctcggtttg ttttgtcaaa tgcagatagc
    29761 ccatgagcgc ttcgaaccct gttgcgaccc gatacgtcgt aacatcagca ttttttgcgc
    29821 tcgtatggct tttagcattt cttcctctgc gataataaag ttcttcttct tctgtcaaaa
    29881 gatgttcttc cagcatttgc tgaatcaaaa aggcttgtgc attcgcagaa acataattcg
    29941 ttgccgcacg atgcagctta ttgggttttg tttctccttg atcaatcagg tggtcacgaa
    30001 catagatttc gtaaatcgca tctccaacat aagccaaggc taaaccgttc aattgtgtgt
    30061 aatctctcat gcttctcttc tccatcgggt tccttgcggt gtatcttcta ataagatccc
    30121 tttttctttc aacaaatcac gaatttcgtc gcttcggaca aaatcacgat tgcttcttgc
    30181 ttgatttcgt tcttcgatca attgttcaat ctcttcatct aataactctt ctgctgtaaa
    30241 taaaataccg aagatcgaaa gccattgcga gaataaagct tctccttgtg ccaagacagc
    30301 ggcagaaact ttcggctggt ctagataagt gttcagccac ttagcaagct catagaccac
    30361 tgtgatccca tttgctgcat taaaatcatc gtccatttct tcgatgaatc gctgttctaa
    30421 attttctaac aaagcgattt tatccgcatc atctgccaaa ttctctgagg cagtttcttg
    30481 gcggaatttc aaattgtcaa acgtattctt cagtcgttgt aaattggtgg ctgcttcttt
    30541 catcgtcgtt tcactgtagc ggatcggacg acggtattgt gtggtcgcca tgaagaaacg
    30601 caagacttgt ggatcaacgt ttttgatcat ttcatgtaca gtgatgaaat tccctagaga
    30661 tttactcatt ttttcatcgt cttcgccaat cgttacatag ccattgtgca tccagtaatt
    30721 agcgaatttt ttccctgttt tcgcttcact ttgtgcgatt tcattttcat gatgaggaaa
    30781 ttctaaatct tgcccgccac catggatatc gatcgtttct cctaaatgtt ttgttgccat
    30841 aacggaacat tcaatatgcc aaccgggacg tcctttgccc catggtgaat cccaagaaat
    30901 ctcgccaggt tttgcttgtt tccataaagc gaaatctaat gggtcttctt tttgctgctg
    30961 ttcttcccct gtacgctgac ttgctccgac ttccagttca tcgatggatt ggtcacttaa
    31021 tttcccataa ttttcaaatt tacgggtacg gtaatacaca tcacctgaag attcataagc
    31081 atagcctttt tcaaccaaca cttgaataaa tgctaaaata tctggcatat gatccatgac
    31141 tcttggatgc agtgtagcag gctcgacatt caacgctttc gtatcttctt caaatgctcg
    31201 gatgaaacga tcagcaactt ctggtgctgt aatacctaat tcgtttgctg tacggatgat
    31261 tttatcgtct acatccgtga aattcgatac gaaatcgact ttatagcctc gatattcaaa
    31321 atagcgtcga attgtatcga aagcaatggt gctacgagca tttccgatat ggatataatt
    31381 atacacagtc ggtccgcaga catacatacc gacatgtccc ttttgtatag ggatgaattc
    31441 ttcttttttt ctggtcaatg tattgtagat ttttatcatt ctgatctcct cactttttct
    31501 cctttgattc gaacgacttt tgctggtact cctaccgctg tagcgtcgtc tggaacatct
    31561 ttcaacacaa cggcacctgc accgattttg gcgttttttc cgatcgttac cggtcctaat
    31621 atctgcgcat tcgcagagag catggctcct tgtttaacag ttgggtgtct tttacccgtt
    31681 tcttttcctg tgccgcctaa tgttacgcca tggaataaaa tcacatcgtc ctcgatttct
    31741 gcggtttcgc caatgacgat tcccattcca tggtcgatga acacgccagt tccaatcgtt
    31801 gctcctggat ggatctcaac accagttagg aaacgccaga attgagcatt gatcttcgcc
    31861 aataaaaaga gccggtgctg atataaaaaa tgagagaaac gatgccaaaa caaggcatga
    31921 aaaccagggt atgtcaaaac gatctcagct gtcgaacgtg cagccggatc gtttctcttt
    31981 gctgcatcaa tcgctcgggt aaaccaactc atgactttcc actcctttcg taagaatttt
    32041 atgtaggaaa tcatcttctt ataaagaaaa gaagcgcctt ttaaataagt ttccttatca
    32101 aaagacgctt tccgcgtggt tccacttttc ttcataatca atgattatct ctgacagcta
    32161 acgcctgtta tacgttcttg gctactttgt ttcctataaa acagtttttc ctgttttctg
    32221 tgtttcgcca agaccactcc cagaggcatt tcggcaacca ttcattgata ccttgcacca
    32281 cccggtatct ctcttgtaat gatcagctgc ttactttttt ctgttcatcg tgtttagttc
    32341 agtacttgat ttaaatgtgc taatgatttt tcacgaccaa gcaattcgat cgtttcaggc
    32401 aattctggac catgcatttg tcctgataca gctacacgga ttggcatgaa aagatttttt
    32461 ccttttacgc cagtttcttt ttgaacagct ttgatggcag ctttgacagt tggagcatct
    32521 actacttcca tttgttctag ctgtgctttg aatgcgtgga gaacagtagg aactgtttca
    32581 ccagcaagta cttcttttgc tgcttcattc aatacaggat gttcattgaa aaatagcgta
    32641 gacagatcaa cgatttctgc agcatagctc atttgaggtt gatacaggct aacgatcttc
    32701 ttcacccatt cgattgtctt ttcatctggg ttttctgcta cacgtccatc agcgattaga
    32761 taaggcagac acatgtcagt aaatgcatct agatccattg ctttgatgta atggttgttt
    32821 acccattgca gttttttcgc atcgaaagct gcgggtgatt tacttaggcg ttttggatcg
    32881 aacattttga tcaattcttg gtgttcgaag atttcgtctt cgcccacagg tgaccagccc
    32941 aataatgcaa taaagttgaa cattgcatct ggtagatagc ctaattcgcg atattgttcg
    33001 ataaattgca agattgattc gtcacgtttg cttagttttt tacctgtttc actgttgatg
    33061 atcaatgtca tatgtccgaa ggttggtggt gtccagccaa atgcttcata gatcatcagt
    33121 tgttttggtg tattggcaat atggtcatct ccacgtaaaa cgtgagtgat tttcatcaaa
    33181 tgatcatcga ccgcaacagc aaaattataa gttggcatac catcgcgttt ttgtatgaca
    33241 aagtcgccac cgatattatc ggattcgaac gaaatagcgc ctttcaccat atctgtaaat
    33301 gcgtattctg tattgcgagg cacacggaaa cgtacaacag attctagacc ttgtgcttct
    33361 ttttctgctt gttcttcagg tgtcaagttc gcacatgttc ctgcgtaatg aggcatttct
    33421 cctcttgcgc gttgtgcttc ccgttctgct tctaattctt cttctgtgca atagcatttg
    33481 tatgctcgat tgctagcaag cagctgatca atcagcggtt gatagatttc tttacgttca
    33541 gattgacggt aagggccgta ttctccagga ttttcaggag attcatccca attcattccc
    33601 aaccaagcaa ggttttccaa ctggcttttt tcgccatctt cgatattacg tttttggtca
    33661 gtatcctcaa tacgaataat aaagtctcca tcattgtggc gggcaaacaa ataattgaat
    33721 aatgcagttc ttgcatttcc aatatgcagg tgcccagttg gacttggtgc atagcgtacg
    33781 cgtactttcg tcattttctt cctcttcttt caaactagac tttgactaac agcaaaaatt
    33841 ccgtcagtta cggcttctca aatatcattt atttaaagcc actcagcaca aattttacct
    33901 tttctaaggt aaatagtaaa gccaactggt tgaatttcct taccagttgg ctgaatttct
    33961 tttttatttt gattgtttag tctttttctc tgtctctttt ttattgtcga ttcctcgtcc
    34021 tgcatgtgca ggtttagcaa agatcatgcg tccagcagct gtttgaaggg cacttgttac
    34081 aacgacttcg atacgttcat tcatgtaatg ctgtccatct tctacgacaa ccatcgtgcc
    34141 gtcatcaagg taggctactc cttgttggcg ttctgtcccg gcttttacta ctagaacatt
    34201 catcgtttca ccagggatga cgactggttt tacggcattt gccaacgcat tgatattaag
    34261 aacaggtaca ttttggaatt cagagacttt attcaagtta tagtcattgg ttacaatgac
    34321 accatctaaa agcttagcca atttgatcaa tttgctgtct acttcactga tatcttcaaa
    34381 gtctccatcg tacatttcga cagatatacc ctcttctttt tgcaaggcat ttaggatatc
    34441 taatccgcga cgaccacgca cacgtttcaa actatcccct gagtccgcga tgtattgtag
    34501 ttcgtaaaga acaaaattag ggatcatcaa ggttccttct aagaatccag tctttgtaat
    34561 atcataaatc cgaccgtcaa tgatcacgct tgtatccaag attttatatt tatggaaatt
    34621 ttctcctgat tttcgttcta acagttgact ctcaacttga ctagctgttt cttgctcgat
    34681 ttttttgctt cgtgaaccaa aaactttctt ccattcttcg attcgtgtcg ttcccattct
    34741 caatcccaga tagcctaaga tgatcatcag taaaatcggc aatacactat tgacgaatgg
    34801 aatttccata ttgtaaagag gggtcgagat cacaacacct aaagtcaatc cgatgatcgc
    34861 accgatcgca ccgaagagaa gataagtcaa gctgacctca cttagttttt gttctacttg
    34921 tttgataata gctgagatat gtttggccaa cagcaaagat aataaaaaga aaataagtgc
    34981 gcctaacagg ctgttggtaa atgtattatt cagccatccg tttgactgtt gaccgattgc
    35041 ctgccaagct gagggtaaaa atgaaatacc caagcttgcg ccgacaatga tcatcagcaa
    35101 agtaatgaca cgtttttgca tcgtttttcc tccgtttcga caatcattta ttagttagcg
    35161 gaacacacgc ttcaacgttt cagccaaagt tgctactcca actatctcga ttccttttgg
    35221 cgcttcccag ttacctaagt tgtttttagg taggtatact tttgtgaagc cgagtttttg
    35281 gacttctttg actcgttgtt caatatgact gacacgtctg atttctccag tcagtccaat
    35341 ttcaccgatg aaacattccg ttggctgtgt tcctttttct ttatagcttg aagcaatact
    35401 gacagcgata gctaaatcaa tcgctggttc atttaatttt accccacctg cagcttttaa
    35461 gtaagcatct tgattctgaa gaagcaaccc agctcttttt tccagcactg ccatgatcag
    35521 agatacccga ttgaaatcaa gacctgttgt tgttcgtttg gcatttccga acatagtagg
    35581 tgtgactaat gcttggatct ctaccaaaat tggacgtgtc ccttccattg ccacaacgat
    35641 agcagagcca gttgcaccgt ctaggcgttc ttctaagaaa atctgtgaag gattagcgac
    35701 ttcttccaat ccatgctcat gcatttcaaa aatcccaatc tcattcgttg agccaaatcg
    35761 gttttttact gctcgcaaaa tccgaaaact atgatgttgt tcaccttcaa aatataacac
    35821 cgtatccacc atatgttcta acattcttgg gccggcgatc gagccttctt tggtaacatg
    35881 accgacgata aaaatggcta tcccatttgt tttagcgatt ttgagtagtt ctgcagtggt
    35941 ttctcttact tggctgacac ttcctgccac acttgtgatg tctggctgcg tcatcgtttg
    36001 gatggaatca atgataacat aatcagggga gatatgttcg atggcacgac tgatttcgtt
    36061 catatctgtt tcagcgtata gataaaattc tgtgttgata cttccgaggc gttccgcgcg
    36121 taattttatc tgctctgcgc tttcttcgcc ggatacatat aagacttttc cgcctatggc
    36181 tgctaattgc tgagaaactt gtaacagcaa agtggatttc ccgatacctg gatcgccacc
    36241 aatcaagacc atagaccctg gcaccacacc tccgcctaac acacggttca gttcttctaa
    36301 ttttgttttg atccgaggtt cttttttagg gacgacgtct gcgatttttg ttggttttgc
    36361 tttttctcct gtcaaacttg tccgagttct gcggtccgtt gtatcttgtt cgatttcttc
    36421 gaccatcgta ttccattttc cgcaatttgg gcagcgtccc aagaacttag gcgacacata
    36481 cccgcaattt tggcaaacaa attgtacttt tgattttttt gccattgtac cgcctccttt
    36541 tatcctaatc atgagacatt aaatcaagct taagtatctt taacgtttta tttactggaa
    36601 gaaccaaatc cgccttgacg ttgagcagtc gtttgatctt gatctgcttt taaaaacggc
    36661 aaaaagatcc cttggccgat acgctcgcct tttttgatca ctacatcctt gaaaccaaag
    36721 ttggtgaatt ggaacatgat atggccttca tttgcctcat tattgtaata atcactgtca
    36781 atcacaccta ctccgtttgt caacactaaa aaccgcttca acggattgct tgaacggtta
    36841 gcaagctgca aaaattcatc ttctcccatg taagctttga ttccagtagg cacgagggtt
    36901 ggtttcaatt gtttttcatt ttctgataca tcttttttca atccatctgc gactgtttcc
    36961 aacccctttt tccagcctgc tttccagata cttggaatca cgacctcttc tgctgcttca
    37021 aaatcatagc cagcagcatg atgtgtagcg cgctgcggta gattgatatt ttgatcttta
    37081 tatttagaaa taacttcaaa tcctcttttt ctcaccggtt ttcctcccta aataaactac
    37141 tcctattcta acatatccga ggctgtaaaa gactttcttt acaaattctt caagaaattc
    37201 tgctatctta aagtaaacca ttaattcgtt ctttttccaa acgactgcaa gttgtacaaa
    37261 aaaactattt tttacatgac tttactcgtc aaaacaagta gaatagaacg aaattaatga
    37321 gtggatattt gattgaggtg tcacatgcgt aaagaaatta aaaatgcaaa acgaattatt
    37381 gtcaaagtag gaacaagtac attgatttat cccaatggaa atatcaatct tgcggcaatc
    37441 gatcagttag cttttgtcct ttctgatctt agaaatcggg gaaaggaagt gatcttagtc
    37501 tcatcaggag ctgtaggcgt tggtatgcat aaattacatt tgtcagaacg tccgactttg
    37561 attcctgaac agcaagctgc ggcggctgtt ggtcaagccg aactgatgaa tatctataat
    37621 caaagattca gtgtatataa tcaacagacg gcacagatcc tcgtgacaag agacgtggta
    37681 gattatcctg aaagtcgtaa aaatgtgagg aatacattgg aacagctttt gattatggac
    37741 acgatcccta tcgtgaacga aaacgatact gtagcagtgg atgaattaga tcatctgaca
    37801 aaatttggcg ataatgatca gctgtcagcc atcatggcaa aacttgtcgc ggcagacctt
    37861 ttgatcgtcc tttctgatat cgacgggttt tattcagata atccgactac aaatcccgag
    37921 gctcagatgt atcgctatat cgataaaatc gatgaaaaga ttctggcaca agcaggtggt
    37981 gctggtagca catttggaac aggcggtatg cagagtaaat taaaagccgc ttctaggata
    38041 tttgaagcaa acagcgcaat gattttagca aatggaaaaa atccggcaat tatttttgat
    38101 attcttgcag gaaaagaagt aggtacttta tttaaggagg acttgaaatg actgacttga
    38161 tgcaattagg acaacaggca aaagatagcg cgcaacaact agcacttatg cctacaaacc
    38221 gcaaaaatga gctacttgtt caaatggcac aaaccatcaa aaagaatcaa caagcgatca
    38281 ttgaagcaaa tcataaagac ttagaaaaag cagtcgaaaa caatatcagt gaaacgatgc
    38341 aagaccgctt gcagctgaca cctgagcgaa tcaatgccat ggctacagag atcgaaaaaa
    38401 ttgcttcgtt agacgatcct attggaaaag tagatgagat gtggacgaac accgacggat
    38461 tgagaatcgg caaaaagcgc gtgccactag gcgtgatcgg catcatttac gaatctcgtc
    38521 caaatgtcac gacagatgca gctagtctag cattcaaatc agggaatgcc gttatccttc
    38581 gcggtggcaa agaagcattc ttctcgaatc aattattagt ccaattattg cagcaagtac
    38641 tggtcagtgc tggcgaatct ccttatgcca tccagtttgt cgatgatact tctcatgaaa
    38701 ctgcaggaca gttgatgcga ttgacagaat acttagatgt gctgattcct cgaggcggag
    38761 caaaattaat ccaacgagtc aaagaacagg caacggtacc tatcattgaa acaggtacag
    38821 gaaataatca cgtatatatc gataaagaag cacagttatc tatggcagtg aacatcatcg
    38881 tcaatgcaaa agcttcccgt ccatctgtct gtaacgcggc agaaacattg ctgatccatt
    38941 ctgagatcgc accttttttc ttgccagcta ttgaaaaaga actggtagaa cacggggtct
    39001 ctttacgtgc ggatgctcgt gcgttagaat atctagagac ggctgcttta gcagaagaca
    39061 cagattggga tactgaatat ctagattata tccttgctgt caaagtggtc gattctttgg
    39121 atgaagcaat tgcacatatc aatcggcata atactaaaca ttcagaaacg atcgtgacag
    39181 atagttatgc ggctagtcaa cgtttcttaa atgaagtgga cgcggcggct gtctatgtca
    39241 atgcctccac tcgttttaca gacggatcgg tatttggttt tggtgcagag attggtatct
    39301 ctactcaaaa gttgcatgcc cgtggtccaa tggggttaaa tgaattaact tccacaaaat
    39361 atatcattta tggcgatgga caaatccgcg aataatgttt cgtgtgaaac attattcccg
    39421 ccttttgcct tttttctgta aaggttctat ccaactagaa aggtgtcaat atgtttgaaa
    39481 ttttaatgat tttttttatt taccttattt ctctgaatat cgctgctttt ctgggagtcg
    39541 gtattttatc ccttttcttt caattcaaaa aacgaagcat agggagtcaa cgggaaaaat
    39601 ggtcgcagta ttttgacaaa atcgggcaaa aagggctggt tacacgcttg cacattagtt
    39661 acatggttgc attatgctta ttagcgacaa tcaattacta ttcttttttc gatcattcaa
    39721 tcgcttatac gattacgcta ctgattgctg ggatttttca cttatcttat aaatatcagc
    39781 tgaataaaaa ccacttgaat cggactttcc gctaaaaaat atccgtacga taagtagagt
    39841 gccttgaaga tcggcaaaaa actgccggac tttttagagg ctgtgacaaa gattttgtca
    39901 cagcctcttt ttccatataa acggcgttcg cctaccagct gcctggtaaa tctgtttcct
    39961 cttcgttaag aagcgaaaat ccgtcaaatg tttcgccatc ataataacga agcagctcat
    40021 ccatggacaa gttcatcgtt ttttcttttt cttcttccgc tgattcagcc agtagctgtt
    40081 cccgatacac ctgatacata ccagctattt tttgctgcat cgcttttcta gtttcacctt
    40141 ccgcattgat tattggcaat tctttgatat agccacgata aatcaaccga tcatctgaaa
    40201 attcaatggg taattcaacg agtgtcaacc cgacttcctg aaaatattct tccagcatct
    40261 attatccccc cattcttttt cttaaatgaa ttttagctta cctactgaaa attttccagt
    40321 ttcattattt tgagaaaaaa agggaatgtg acaaaaatcg tgtcacatcc cctttttccg
    40381 aataaatggt gttcttctgc caactcctca atcataagtc acaatattca acaaacatga
    40441 gaaactgttt tttgaaattg ttccttgtga ttcagagtta aacgacagtc tcacaacctc
    40501 ttggtaaacg gcgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata
    40561 tgagaagcta tttttgtttg gctgttctta tgacttgatg caggacagct ttttcacaac
    40621 ctctgatttc ttattcttct tcatttgtag caaaataatt acggtgataa atgatacaac
    40681 atacactgat aagaacaatg gcacctgcaa aataaaacgc atagctttca ccaatatgtt
    40741 caccaatcac acctgcaata aatggtgcta aagctccacc gaaaaagcgg acaaaactat
    40801 agctggacga agcaacgttt cgttgcagac ctggtatttc catagcaatc gtcgtcaaca
    40861 gtgtattgat aattccttgg aaaaatcctg ctaacacaat gctgatcggt acaaggactg
    40921 gcatagaagt tccagctcct aataacagca aacaaataat gaataagaca aatgctgcta
    40981 aaattgtccg ataagtcgta aacttccgct caagagccgg tgcaacgaaa atcgaagcaa
    41041 tagccagaag cacaccccag ccaaaaaata caaaaccgac ttctaattca gaatagccaa
    41101 tcaataaaaa tggcgcatac gcaagtaaag taaagaatcc gaaattatat aacaatgcga
    41161 ttagtccaac tgaacgaagt ttatgattgc ttaacgcttt gacaccttcc caaaggccta
    41221 cttttctctt tggtttctca gtttcaggta aaagtaccgc tagggctata aaaccgcaaa
    41281 acatcaaaaa tgctacccca aaaaatggat aacgccatga accactgccc aagaccccac
    41341 cgactagagg gccaacagcc attccgcagc caagtgctgc ttcatacatc atgattgctt
    41401 tttctgtgtt accaactaaa atactgacga tcgttgctaa agcagtagag acaaataaag
    41461 cattcccgac accccagccg gcacgtaacc caatcagcat tccgatattc ccagataatc
    41521 cgccaagacc agcgaataca acgatgataa acaatcctgt catcaatgtt ttttcaaacc
    41581 gatccgacta gataagaaac ctgtaaatag catcaccaca ccagtgacta acatgtaact
    41641 agtaaataac agggtcgttt cagctggcgt agcgtttaac tgtgcagcaa tcgtttttaa
    41701 gattgggtct actagaccca ctcccataaa agcgatcata caggtagctg ctacaactag
    41761 cgctgaactt ttcccctttg tttcttcatt tgtttgttct tgtatatcta tttgattctc
    41821 caatatttcc atccctttct ctctattagc atcatacatg atttatttta catgtaaata
    41881 tgcacatgta aaacgtggaa actttactgt gatataataa agaaaataag acgagaggta
    41941 ggatttttat gaatacttta gcctatatat tattatgcgt gcttttatcc aaaccacgtt
    42001 ctgggtatga attgaagcag ctgatcacaa ttttttggga agcccatcac agtcagatct
    42061 atacaacgct tgccaagtta gaccagcagg gttatgtgac cgttttggac aaagacgagc
    42121 actctcaaaa aaagatctac cacttgactg atgaaggacg tgctcttgta gaggaatgga
    42181 tcaaagagga aactcccgct cctacgcaaa aagatgaatt tctagcaaaa atctatgctt
    42241 tttcgacgct cgataaaaca acagctactg gactgctatt cactagagaa caacagctga
    42301 acaaaatcat gcaaaaaaat caaaaaaaat tgaatgacct ctcgctagac aagaaacaag
    42361 aatttggacg ttatgttgtg atccagcgca gaattatgct ttgtcagcag gaactccaat
    42421 ggtgtcagca agtaagagaa caaatgcagc agttttttga gtaagggata aacagaacct
    42481 ttctagaaat atctattgaa gagcaaatta tcttcaataa aagagcaata cagttggaaa
    42541 agaaaaagcg atccgaacga caggccaaaa cctatcgttc ggatcactat ttttctagaa
    42601 ctgcattcaa gagactatga caaaaagcat gccaaggcct tttttcaaat aacagtgttc
    42661 catcattcat tactttgaat tgcttttagc acacttcttg aaagcgggcc aacgatgatc
    42721 aactgcaacg gtaaagcggc aatcacattc attttccaag cattcatata agcagagaat
    42781 aattgatcag gtattccttg ttccatgatc acaccgaata aagacataca tgtcaccatt
    42841 cctaaaacca tcaagcatga aatagttaag atcatttgga ccttgctttc tggatttact
    42901 ggtagtttaa aggcaatttt tttcgccagc acaccgatga tgaagacatc caaaatgaaa
    42961 gccaccacga atccaggcac tagaccaatc aacaaacttg ataaatgaag atcttgatgc
    43021 agccataaat tatacgcgct catccccaag accatcaaaa aacacatcat tgttgtaaaa
    43081 attattcctt cttttttatt tgtaggcaat ctgtaccact cctttaaatt tgtcacgaaa
    43141 atcatcgtag cacatttaaa aatttctcgt aagtactttt tttattttct gcccttttat
    43201 ttcataaact tcatcagccg tacgctcgac aaagtaagaa tcatgagaag taaacaaaat
    43261 cgttcccgga tatatttgca ttagcttctc taaagcttcg atcgttgcca tatcaataaa
    43321 attagtcggt tcatctaaca gcaacacatt acttggctta gtaaaaagca aagcaatcgt
    43381 cagacgggtc gcttctcccc cacttaatgt acataacggt ttcgtcaaag tttcctgagc
    43441 aaaccctaag tgattcagca aactacggac aagcgattct gaaaactctg tttgtctcat
    43501 gacaaaacga atgatggatt cttcagatgt catttgataa cccttttgct gatatacttg
    43561 aaagcttact ttcggagaga gaaggattcc ttctccttgt ttgcggatat gttctaataa
    43621 ggttgtctta cctgaaccat tttcaccgac aatcgcgatt tttttaccta aagaaaactg
    43681 aaaatcacca tttaccagta atgttctccc acttctttcc aattggacat tttgtcccat
    43741 gatgggatag cggctgtgga gttcaagaga ttttggtact ggaaaacgga tttgtttcat
    43801 ctgctgcggc ttggttgttt ctcctatttt ttggagtctt ctctctaatg ttttcgcatt
    43861 cttttggatg gctttttgta cactgtcttt ttgtttagag gaagacaacc gatccggtct
    43921 gatttgttgc tttttctttt tactggacac tttttccaac ttttccgctt gttttttctt
    43981 ttcttggatc gatttttcca atcgtttttt ctctttcatg atcgactctg cttctcgtag
    44041 ctgagtcttt ttctccaatt ccttttgagt gagataggca ctataattcc ccgaaaactc
    44101 tcggatttct ccgtctttta cttcccagat tttcgatgct agctcatcta gaaaatagcg
    44161 atcatgacta acaaaaacca gcgtaccata ataataacga agctcttcaa tcagtcgatc
    44221 aatacttttt ctatctagat gggttgttgg ctcgtctaga agcatccctt gttcataaga
    44281 agaaagaaga cgtatcaact cgatctttcg ttcttcccct ccgctccatt tccgctctga
    44341 agtttcttcc agtcttaact ggaaatgact ggctaattcc aaatttaggt cctttgcttc
    44401 agcgagatag gttaattgag gcaggtagtt gaaggtgatc tctgtttgga ttttcccatg
    44461 atcgggaaaa agttcaccgg ctattagttt gagcaaagtc gatttaccga caccattttc
    44521 tcctatgatc cctatacgat cttgctgata gacacgaaga gaatcaatag atagttctag
    44581 ctggttgcca aagctgactt gtagatttgt tatatttact gctaaatttt ccatatgttt
    44641 tacactccta tttcatgtaa aacaacaatt atcgatacta aaaaagagca gaaaaatctg
    44701 tttgcaaaaa aactgcatca catcttttcc tactctgaac gaaataacta tgactaaatc
    44761 accaaatctg tcatagcttt cacctattct actgtcgttc gaaaaaatgg acatactcca
    44821 tccatgaaat tgttgtttat acatgtcttt tgaatttatt tagttgttat tcaaaagttc
    44881 gaaacgtaat ttcatcgatg cagtcatttt tccgacctcc ttttttatta ggttaccaca
    44941 cacgattcaa aaaaagcaat agctactgaa tagtaactga aaacctttta ttcgaacgta
    45001 gtgatctttt ctggtgctaa acgaacagac tcgtaacctt cttctaccgc ttttcctatt
    45061 gataaaataa taactggaac ataacggtct ttatccaaac caaatgcctc tgccaactga
    45121 tctgcttcga atccaccaat tggatttgtt tcatagccat gatcacgcgc aactaacatg
    45181 aattgcatag ccgctaagct agaatcgact ttgactacat cgttcatttc ttctcttgaa
    45241 aaactcttgt aataaggaat gatcgcagct aattgttgat ctctgacttc ttttggcatc
    45301 ttccctgatt cgtaagcttg gttatagatt tcttcgccat attcgtaaca ttgcatatcg
    45361 ccaaaaatca tgaccattgc agaagaagta tcattttgtc tcgtattgaa acggatcaaa
    45421 ggacgcaaga tttcttttgc ttcctcgctt tcagcgacca caaagcgcca tggttgcata
    45481 ttgacagaag aaggtgcagt cgtcgcttct ttgatcattt ccagcatttc ttcgtgtggg
    45541 attttgtatg tttcatcata tacgcgaacg gatcttctac cagtcataat tgttgaaaaa
    45601 tcattttttt gttccattat tattaacctc tttcgtttct gttgttatat tgcaaagttg
    45661 ggtattcaat ttatctaaaa gtgttagaaa ttgtttcatt tctacatcgc tgaaagccgg
    45721 ggatagcacg gaacctgtat ctttatggtc catttcacaa tgctcgattt tcttctgggc
    45781 tttttctgtt agcgctacat acacttcccg gttgttctga ggatttctct gacgcgaaac
    45841 atagccattt gcttccaata ccttcagatg acgagtaact gctccttgat caatctgcaa
    45901 cttttcttgc aaactgcttt gtagcaatgg attttcttct ttcagtctca tcatgatctc
    45961 gtatctagta agacttatac cgatattttc ttcaaagatc tggacgattt tttgatcggc
    46021 taatttgatt tgatataaaa ggtgactaat ctcttctaaa gacataattt cctcctttct
    46081 aataattgac tcgtcaacga ttgacttgtc aataatagaa taaaaaaaga aaaaagtcaa
    46141 agaatcagct gtgtaataaa aaaaacgctc tcaatagaaa agtatagaga aaagaaattt
    46201 tttgtagaat gaacggaaaa aatgataaag aaagcacaac gctaaaacca agattattga
    46261 ttcttatcaa actatagaag atagctcttc tgcagtagaa aattttacac cgccaaaaat
    46321 ttccgaaatc gccttttgca agaatcccgt cgaatattag aaattggatc agtatgctta
    46381 aacagtaata aaaaagttat gacaagatgt tgtcacaacc ttttttgagt aaacggtgtt
    46441 caaaaacttg catctgcggt aataagttca accaaacaaa aaacatgaaa atctgttttt
    46501 gtttggctgt tcttactact tgatgcaggg cagctttttc actacctctt tttaataacc
    46561 ataaaatcct attccttcat atctccagcc catcggtgtt agccaatctc tttccttagc
    46621 actcaaggta tagtgatggg taccagtttt ttcatttgga ttaaatagtc gataaattgg
    46681 cttggaacca ccagaataaa aacttagacc ttcataacgc caaccgattt ttgttaaaga
    46741 gttgatctca gattgtgcca ttgtgtaatg atggtctcca ctgtaaggat tgtataaacg
    46801 gtagacaggt tgtccgctgt ttggtgcttg ccaagcaatc ccttcatatt cccagcctac
    46861 agttggcaga taattttttt ctccggcatt caatgtatat aaatgttctc ctgtatgacg
    46921 gttatacaga cgatacatcg gagaagttga agttggtgct gtatcatact gtgtcagatt
    46981 ataggtttcg attacattgt tcaatgtaga accataatta ggcgcagtgg cataacgacc
    47041 tgttagccaa gccgtagcat ctttgtaaga gttcgtattg cttttccatg caccggaata
    47101 gtagtaaacg cctggctgaa aagaagtcgt cttcaatacc gttacgttat cttgcaatga
    47161 ttctttgtac gaaggatatt ttctaaaagc ggcattcacc gtaatccatt gcccattcac
    47221 atattccgaa gtaggcatcg taacagattg gccattatag cttcctttaa tcccaaatag
    47281 attgtaatta ggggcagaag ccaaagtact gttcccccag ccactttcaa cgatggcttg
    47341 agcaatcatg actgatgcat ataagtcatt ggcacttgcc aaactttgtg cagttgaggc
    47401 aatactatca atgaacgctt gttgagaatt agcagatgag cgagcataaa aagcatagcc
    47461 agtatagttt tcgaattcag acaaatctgc ttgatgggta tgtgtcaatg tttgcccatc
    47521 tgtcaattgg ttcgtgattt cttctgcatt ctctggattg ttctgagctt ctgctgatgt
    47581 actgtcttcg gtttgtgttt gttctgttgt ttcagttgtt ggttcttcgg ttgaaattgt
    47641 attttctgag acagtagctg actgcaaagt actctctgta tcgttttcaa ttcctgtttc
    47701 tgaagaactc gatgtctgag ttgcgacagt ttctgtcgat tcttcagcta gcactgttgt
    47761 gttggcaact gctagatttc ctaaaagaat aacgctaaat aataaattct ttgctttcat
    47821 cttttttctc ctttgaatta tgaaactttt cctaaataac agtgtaacta aaaaagtaaa
    47881 agcaagtcaa tgtccatatt taaataacat ttgtttcttt ttgataaatt tgctttttct
    47941 acataaaaag acgtaaatga agaatattct tctccattca cgccttaaat tgatggtcgt
    48001 ttgaataact atttaacaag taattctctt tggatattat ccgttatgga ttatacttcc
    48061 tgtgtcgtcg gacggataat catgttactg acagacatcc ggtctggcgt ttctatcgca
    48121 aatgctacag cagaagctat atcttcggag gtcaatcccc attctttttg cgcttcatga
    48181 agttcaagag cagccttttt gtcgctgatg gtttgatata attcagtttg aacagctcct
    48241 ggagagatga tcgtagattt gatgttattt tgccgttgct cttgacgtaa tccttccatg
    48301 attgcacgga cagcaaattt tgttccgcaa taaacggcgg aatcaggata aacaacatgt
    48361 cccgccacag aatcagttga aatgatatgc ccagattttt gtttttccat gattggaagt
    48421 acggcggaaa ttccatttaa cacacccatg atgttgatat caagcatatt ttgccattca
    48481 tcacggtgac cttcaactaa tggagcggtt ggcatgattc ctgcattatt gtacaataca
    48541 tcgattcgac cgaacttttc catcgctaaa tcgataactg cttggacttg tgcaaaatct
    48601 cttacatctg ctgtttgaat atatagctca gcttctggta aagattcttt tattgctttt
    48661 aggcgatctt ctcggcgtgc agctataact agtttagccc ctttttctgc taatagcttc
    48721 gttgttgctt caccgattcc gcttgaagcg cccatgatga caatcacttt ttctgataaa
    48781 tcattcattc tattctcctc ctgttttgaa aaatctcttg cttatactca ctatctctta
    48841 tttcatctta ctgttcaaat aatccgttcg acaggtttga cgattttggc agggattcct
    48901 gcaacgatgg tattgtctgg gacatctttc gtaacagttg cattcgctgc aacaatggca
    48961 ttctcgccaa tcgttacgcc tggcagaata gttgctcctg ctccgatcca agcatttttc
    49021 ttgatgtgga ttggctttac tgacaagcct cttcgttctt ttggatcggt tagatggttc
    49081 accgtgatga ggttgacacg aggaccaatc aatactgaat cttctaaaca gatgccacct
    49141 aagtcaacga acatcacttg acgattgata aagacatttt tccctatact aatatgtttg
    49201 ccgaaatctg tataaaaagg cagcagaaca gtcaccgtat catctacttt gtcactaatg
    49261 atattttcta gccgtttgcg gatatctgct ggttcatggt acccagtatt cagcgctgct
    49321 aaaggctctt cgttttcagc ttggatctga tggatcactt caaagagcgg atccgttact
    49381 cgaatctctt tattgacgat ttgtttttcg atgaggcttg tcattttgct ccctcctttt
    49441 atccttactt tataacggac ggcttatcat gtctaatact tataaaacat acttaactat
    49501 tccttttaac tatacttagt ggtatgcttt tttaaaaaca ggagggaaaa aatggatatt
    49561 cgtatactga cgtatttttt agcagtagct agagaaaaaa atatcagccg agcagcaaaa
    49621 tctctgcata tcacgcaacc tactctctca aaacaactga aagatttaga agaagaactt
    49681 ggtgtgacac ttttcactcg gggctcccga gagatacagt tgacggattc tggtcgatat
    49741 ctttattcaa aaggaaaaga aatcttgtcg ttggtagaaa aaacggcgaa caatttaaca
    49801 aatgacgcaa tcatctctgg agaaatctac attggcggag gcgaaactaa agcaatctct
    49861 ttcatttctg atagccttca ttcgttgatc aaggcgcatc ctgatatcca gtttcattta
    49921 tatagcggga atgcagatga tatcatagac aaactggata gcggattact tgattttgga
    49981 ctcgtaatcg aaccgacaaa caaacaaaaa taccagtcat ttcgactgcc agcccaagat
    50041 cgttggggac ttttgataca tcgaaatcat cctttagcaa agcaaaccgt catccatccg
    50101 caggaactga aaactgttcc tttgatcatt tcccggcaaa cgtcagtgga cagtcaacta
    50161 gctgaatggc taggtttcag catagatcag ttatcgatcg tcggaacata taacttattg
    50221 tacaatgctt ctttgatggt cgataacggg acttttggtg cattagctct agatgggatc
    50281 atcaatactt atgggacgga aaaaatattc atcccactcg atccgccatt acaagcttca
    50341 ctgaatctca tttggaaaaa agaccagcct ctttctcctg ctgctcgtaa atttttagat
    50401 gtcctaacgg agaaatacag ttaagtagag tttttttcct tcgctttcag ctttttaaca
    50461 acgaataaga taagcaaaag atccaacgat taaataacat aagtttctta atttatttct
    50521 tccttttatt tctgataatt ccagtaaatt actataatta taaaaaaaga attgacaaaa
    50581 cataactgtc tatataaaat tgattacgtt aacaataatt cgtttcatgt gactagatca
    50641 tactaggcat gaaaaatctc tgaggaacat ggtttcccta ggaggttttt cgtgtctatt
    50701 tttgtttggc cgcaatcaac tggtctagca aacgtgaaaa ccaaatcttc aaaaagggtg
    50761 cgcgcatgaa aatcaaaaag atctttaatc aaaatgcagt attggtagat gacggtggac
    50821 aggaaaaagt agctattgga aaagggattg gatttgacag aaaaagaaat gatttgattt
    50881 ttgatcgtga tgtcgaacgg atatttgtca tggaaccaga aggtcaaatc aagttgcaaa
    50941 atttacttaa tcagattgat gaacgctttt tatttgcagc agaaacgatc attgatcatg
    51001 cggaaactgt tttgatggaa aagttgaatg aacatgtatt gatcgctttg accgaccaca
    51061 ttgctttttc tgcggagaac attaataacg gtatcgtcat ccgcaacaaa cttttaagag
    51121 aaatcgaagt gctttacagt gatgaattca gtattgctca atgggcagta gagtatctga
    51181 caaaagagct tgggattcca tatacttatg acgaagcagg atatatcgca atccatattc
    51241 acagcggacg aagcggtcgg aacaataacc atcgcagtat ccgtgaagtg acgattgtat
    51301 ctgacatcat ccatttagtg gaatcagaat tggaaacaga tatccattca gaaccatttt
    51361 cactgaatta ttcaagactg gtgaatcatt tacgactgct tctacaacga acccatgcgc
    51421 aacaatatgc tgttttagac acggaaatcg tagaaatggt caaacgaaaa tacccagaaa
    51481 gctataagat ttctaaaaaa atccgtgtgt tgctgaccaa agaataccag ctcgcgatca
    51541 cgaaagaaga actgggttac ttggccatcc atatcgagcg tttaagaagc gcaatcgtac
    51601 aaacaaatgt taacaatcat gaggaggaaa aaaattgaaa gcatatctac aacgaatggg
    51661 acgttccctt caacttccag ttgcggtctt acctgctgct gctttactag tagggatcgg
    51721 aaactggtgg gcatcttata gtaacgacat tgtcgctcat tttttacaag ctggcggtaa
    51781 cgcagtttta ggtcaattac ctttgctgtt tgccgttggg ttagcactag ggatgtcagt
    51841 ggacaaatca ggtgctgctg ccttggcagg tttggtcgca tttgaagttc cagtaaatgt
    51901 tttaaaaact gattcagtag ctactttatt gaatatcaaa ccagaatcag ttgatccatc
    51961 ttttgctcag atcagtaacg tatttattgg tattattgcc ggattgattg cggcagctct
    52021 ttataatcgt ttccacgaag caaaattacc gatggcttta tctttcttta gcggcaagcg
    52081 tctagtgcca atcttggctg cctttgtcat gttgctgatc agtgcagtgc tactgctcgt
    52141 atggcctccg gtttactctg ctttagtttc ttttgctaaa ttcattttga gtttaggctg
    52201 gggtggtgca ggtctttatg gcttcttcaa ccgtttactg atccctacag gattgcatca
    52261 agcaatgaac tccgtgttct ggtttgacgt agcaggaatc aatgatattg gtaaattttt
    52321 ggcttctgaa ggaacaaaag gcgtaacagg aatgtatcaa gctggatttt tcccaatcat
    52381 gatgttcggg cttccagcag gagcatatgc tatttacaga aatgcacgtc cagaaaacaa
    52441 agcaaaaaca gcttccttga tgctagcagc agcttttgct tcattcttta ctggtgtgac
    52501 agaaccactt gaattttcat ttatgttcgt tgcttggcca ttatatgtct tgcatgcaat
    52561 tttcactggg atttctttag cagtgagtgc attcttccat tggacagcag gatttgcctt
    52621 tagtgccgga tttgtcgatt acttcctatc tttgaaaaat ccgattgcga atcagccatt
    52681 gatgttgatc gtccaaggat tagtaacagc agtgatttac tactttggct ttaactttgc
    52741 tatcaaaaaa ttccatctga tgactcctgg ccgcgaggaa gcagatcttt cagatgacga
    52801 tactgcaacg actaattcag acaataaata tgcggcacaa gcaagcaaaa tctacgctgc
    52861 attaggcgga gccgacaatg taacttcgat cgataactgt actacacgcc ttcgtttgca
    52921 agtcaaagat acaggcctga tcgatcaaaa taaaatcaaa gccactggcg tacctggtat
    52981 gaagatcatc gacggaaaaa atgcgcaagt catcgtaggt acagaagtac aattcgttgc
    53041 agatgagatg gcaaaactac atggtggtgc tgcagcacga cctgcacaaa caaacaccgt
    53101 ggttaaaaca gaaacatttg ccggtgaaac gatcgaacaa gatatttacg ctatcgcaaa
    53161 tgggaaactg attccaatca ctgaagtttc agatgatgta ttcgcggaaa aaatgatggg
    53221 tgacggctat gctgtattac cagaaaatgg agaaatcttc tctccaatcg ctggtacgat
    53281 cacaaacatt ttcccaacga aacatgctgt cggaatccaa acagatgctg gtatcgaagt
    53341 cttgcttcat atgggcatca acactgttga cttaaaaggc gaaccattca ctttatatgt
    53401 agaagaaggt caaaaagtcg caagaggtca attgattgct ttagttgatc ttgccgctat
    53461 tcaatctgca ggcaaaaaca cggatatggt cgttgtattc acaaatggcg acaaagtcca
    53521 aagcttagaa atcgaacccg cacgtgatgt aaaagcaaat gataaaatcg gctctgtttc
    53581 caacaaagcc tgagccatcg tcttccccag acttttttat cgataaacaa acaggagctg
    53641 agacaaaaat cttgtctcag ctcctttttc cgaataaaaa cggcgttctc ctgccaactc
    53701 cgcggtaata agtccagcta aacgaaacat atgaagagct attttttatt tgactcttct
    53761 tattacttga tgcagaacag ctttttcaca aaatttttct tcatactcaa aactaatcgg
    53821 ctttttcaca acctctttat acattattcc tgatccaata tttcttttaa tgctgcttca
    53881 agagaagtta atggttttcc caatactttt tctaaatcat cagaggaaat agctaactga
    53941 tcctttttaa tatcatgttg gatcgctaaa aacatgtcag ctgcttcttg agggaatccg
    54001 gcttctttta gattttcgac aaacgctgca tcacttgctt caataatttc taactcttta
    54061 cctgtcgcac gctctaatgc cttggctaat tcttcatagt cagttagatt gccggataat
    54121 tctaagattt ctggagagtc actttctact agtacttttg ctgcagcttc tgcatattca
    54181 cgtttcaatg cccaacctgt ttttccattt cctgcagcat aaacgaattg accagttttt
    54241 aaagcctgcc caatgatagg taattcattc tctacatacc aattattccg taaaaatgta
    54301 tgggaaatac ctgaatcctt aatcattttt tcagtgaatt ggtgatctgg cgctaataca
    54361 ctgatcgagt gatcggcatc tgcaaaactt gtataagcga tataagatac cccagctgct
    54421 tgtgctgcct tcactacatt cttatgttcg gcttgacgat ttcctggaac gccagagaca
    54481 aaaagcaatc gatctattcc tttcaacgct tctttcattg agtctaaatc atcataatca
    54541 ccaatacgta tattaaatcc agcttctttc agtgcttctc ctttttcttc actacgtact
    54601 agtacataga tttcattttg tggaaccatc ttttttaaga attccagcgc ataaccacca
    54661 aaacctccag ttgctcctgt tactaaataa tgcatatttg ccactcccta ttctttttat
    54721 ttgttaagat ttacagctgt acatttataa attacacctt tatcataaag agtgtggtaa
    54781 gataagtcaa gcaaaaaaaa tgaaaggatg acaactgatg aagtattctc tgcaatttag
    54841 tgatgcaatc catgtacttg cctatattga aatgtttcaa gatacccgat cactttctag
    54901 tgagatgatt gccagtagta tcgaaacaaa tgcagcaaat gtacgtaaaa tcatgagtca
    54961 gctcaaaaaa tccggtttga tctttacaaa aaccggaaaa ccgcagcctt cccttgccaa
    55021 aagtccagat cagatttctt tactagatgt ctatcgcagc atcgaaggta atacacagct
    55081 tatccaagtc gatccaaaaa caaatccaaa ttgcgtcgtt ggtgcgaata tccaagaagc
    55141 tttagaagaa agctacgctc ggctgcagaa aaaggcagaa gaagaaatgg ctaagatcac
    55201 acttgatagt ctgatacata aaatagccgt tttggaaaaa gaaaaaagac cagaaaacac
    55261 aggcctcgta gagagatttt tataattctc tctactgtgc ctgcattttt ctgatcggaa
    55321 tcgtctacat aaattctttt attctccggg ggacaaaagc cttgtcatgc ccctttttcc
    55381 tctctaatgc tcaggtcaaa gcgctgaagt catgacgtct tgataaacgg tgttcaagta
    55441 gctgaccctc ggcattagtt ctgaaaaatg caaaatatgg aaagctattt cccatttttc
    55501 ctctctaatg ctcaggtcaa agcgctactt tcacgacctc tatattatca tggtttattg
    55561 actaccggta tccagatttc tcccatatat ttcccgttgg taactcccat ttcgactaca
    55621 gcatttggac ccccaacata ggcgtaatct tctacttctc ttaaaacatg cccaaaggcc
    55681 gtattcgtca aatcatttct taattcttca ggcgattctg ctaaagcgct tacaacaaga
    55741 tacgttcctt ttgggaatgg gatgattcgc gtagtgtttg gaacatcctg actcgtttga
    55801 acaccgacat agtacatcat tttcccgtta tagggttcgt tgactgtaaa gaaataatga
    55861 ttttcagcag cttgtttcaa tgcatctatt gttccattac cgataacttt ttgctcaaat
    55921 gcttgtttct ctgcagccaa accgacaaaa tccgtattgc tactcttcaa ttccacacca
    55981 atacccgtca ccgtaaaatc tggtttttct tcgatcctat actttgccat ttctcccacc
    56041 ctttctatta tattaagttc atcttacata aaattaaagg gaaatgacac gattacgctc
    56101 ttttttctag ggatattttt attttttaac tatttttttg ctttcactgc aatccaaatc
    56161 tcactgtaaa cattactgaa atcctctccc cattttgtaa aagagatctc gggagcttcg
    56221 actaacccat aatctgaaga aggaagccat tctgcataga ttttccccca agtatcttgt
    56281 aaagtttggg gaaatggccc tttattagga aaaatggccc aagtgtgttc ttctatagca
    56341 agcatttcta aatcagcgta cggattttct tttgtcgtag caaagccaat catgtgcatc
    56401 aaactgccct tttcttccaa gcggtctcca tcaaatccat aagaaacgtt gattacttga
    56461 tttggatata aatctcctaa cttatgcatc tcttcccttt gagtctccgt gatcgtctgc
    56521 gctaactttt gtatttcttg attgactcct tcaaatttga caggaacttt tttcgccaca
    56581 cctgccacat aaaactgttc tttcttttcg atacgaaatt ccatcgattt tcctcctctg
    56641 acatcaataa aaaatgtcaa gcgtggaaaa gatttttgta tgccgtatct actgatttct
    56701 gaaggcagat atcctgacca atcacgaaaa gctcttgaaa aaccttcgac tgtttcatat
    56761 ccatatctaa acgccgtttc tgtcactctt gatccatcta gaagatcgcg attagccaaa
    56821 gccaatctac ggtttttgat atagtccatt agcgacatcc ctgcaataaa cgtaaagatg
    56881 cgttttaatt gatattcaga gattcctgta ttttcggcta ttattttata ggaaagtcct
    56941 tctgtcaaat gttcttcgat gtaatcgatc aattggttca gatctttaag catatttatc
    57001 ttcctccttg attgcctttt tatcatatta tcaaagaaag tcaattactt gatgaatcag
    57061 gaaccaattt agtcagatgt agaaaatgtt gtatggatct aaagaaaatt ctcaagcagc
    57121 gtttcgtaac ggataattgg tattatctgc ctatctatgt tttaccttta gaaaaataaa
    57181 ttttggggaa gacagattct aagtaaacaa agcggtaaaa atatctttta ttcccttagt
    57241 taatccgctt actacctact gtacaaagca aattgttgtt caaactgctg atcagaacgc
    57301 tgcttggatt tttctttgcg atacatcata cctcttttat caattggaac aattatttat
    57361 ttctaacaaa atcacttcat ttttgtcaga taaataaaaa acaaaaaaag ttccatcttt
    57421 aaaacacgct attcttttac tttttctcac attctctaag aaaagaaaag gagaatgaat
    57481 ttttgacaat tcgagcaaaa gtgattatca aatatgtaaa atcagctact tttttcttaa
    57541 acgtttcttt atactaaata taaataagtg tttcatgaaa ggagaactag atgaataatt
    57601 ggcttgagta ttttcctgaa aacgttttgg aacgcggtta cagctatcat ctgcacggtt
    57661 ttgtccgtca tttgaactat acaagcaagt acttgtccgc aacagtttct ggcacggaag
    57721 attataaggt agtgatcact tgggatgaaa agaccaacat gacttgtgat tgtctatatg
    57781 ccatagaagg aaaaaaatgc aaacacatgg ctgctgtctt attcgcttac gaagaacgtc
    57841 caataaaaaa atccaactac tcactttccg aactctcgtc ccttgtttcc tccgctagtt
    57901 cctctcttgt acgggagctt ttaacagaga ttcttataga acatccgcac tttatcgaac
    57961 gcttcaaagt taagatgcca tttcatgcaa taaattattc agataaatta acaactatca
    58021 tccataaata cgatcatatt atcaagaaaa acaaaaacag gaaaacagcg aagttcatca
    58081 tggagatgcg aaagttcatc caagaagcag ttgaatcgct tattcagcaa aatgcctatc
    58141 ttcccgcatt cgaattgatc aatgaagtga ttgctactct cgaaacattc tattgggagc
    58201 ctgaggatga acgaaccctt cttctgatag aggactgtta ttatttatgg aaagaattat
    58261 tagcagaagc acctcatgca gaaaaaaggc aaatgttctc ttggttcgtc tgccaagtcg
    58321 atcatactga cgcctcttat tcaaaacgct actctataaa aatcctgaaa gaagactttc
    58381 gggaaaaaga attttctaac caaaagaaaa aaatagataa aaaactaaag aaacggtaaa
    58441 gaaagacgat ttcaatgaaa aatagcaagt aaacgataac aaccatccta aatccacaag
    58501 aaatacagca gaccttgctt taaaaacacg aatcatgcaa aggatgaagt aaagaagact
    58561 taacgcgtac ccaatattag tcatttgact attaggtacg cgttttgttt tataagtcat
    58621 gacaaatttc cttcgttgct ggatagattt tccggcattg ttgatagatt ttatgatact
    58681 tttctacatt ttgtaagaga ggttccgctg catctgtata ttggataaaa ttctgacaac
    58741 aactttcagg tgtgtcgaac caccctaacc ctagtgcagc aatcattgtt gcgcctaatg
    58801 caggtccttc ctccatactt aaacaataga ccgttgcatc gaaaatatca gcttggattt
    58861 gtagccatgc aagatttttc gcgcctcctc ccaccgaaac aatacgtgta aatctgcgac
    58921 ccgctagttc tttacgtttc aatcccacca ttgacaaatg ctcattaaaa atttagaata
    58981 ttcacaagtt taacgacaat tatgaaaatt tatgtcgtgc taaaggagtt ttgttattgg
    59041 atgaaaagtt agatataaaa actgcgatca aagacacgtt acctaccgtt ttcggttata
    59101 tcggtattgg acttgcattt ggtatcgttg ggaaagctgc cggatttcat ccactagtcg
    59161 tcacgttgat gtccctgctg gtctatgctg gttctgccca atttatcaca gtcagcatgc
    59221 ttgctagtca cagcccattg ctttccatcg ttttctcgac ctttctagtc aattcccgaa
    59281 tgattctgat gagtatgacg attgctcctt atttcaagaa aaatcggctg cttcaaaatc
    59341 tgctgattgg cacattgctg acagatgaaa gtttcgcact gggaatgaat aaattgaact
    59401 atactggaca aaagttaaac tttcgttgga tgaatacagc taattggatt tcttatttga
    59461 cgtgggtggg ttcttctctt gttggcgcac ttttagggaa ttttattact gatccgaaaa
    59521 agtttggttt ggatttcgcc atcgtcgcta tgtttatcgg gttactttat cttcaagtca
    59581 tttctgatag aaacacctct aaacgattac agttgatcct gattggtctc actttgattc
    59641 tggtatatgt cggactgatt tttatcccaa gcaacttagt gattgtagtt gtaacactga
    59701 ttgggtgcgg attgggggtg tggatcaaac atgccttctt ttgagtttac attgctgact
    59761 attttaggtt gtactttagc aacttggctt tctagggtcc taccatttgt cttactgaaa
    59821 aaattcgatt taccgcaacc gttgcttgag tatttaagtt ttgtcccgat cgtcattatg
    59881 tctgctttat ggttcagcag tctatttacc caaaatatcg gacatctgcc gcagatcaat
    59941 ctcgaaaatg ctttggcttc agttcctaca cttttagctg ctattctttc aaaaagctta
    60001 ttggtgatcg tcttggcagg aattctttcc ctttcgttga ttcgcttatt ataaataaaa
    60061 aaacaagagc ttatggcaaa ttactgtcat gcgctcttgt ttttatcaaa gtcaaataag
    60121 aaaaaaatcg gcgtattctt gcttatccgt tgaacggacc actaacaacg acttttccta
    60181 acatatgtcc tgtttctacg tctttggttg cttgtttcaa gttcttcgca ttcatcccat
    60241 ctgaatagac cttgctcaat gtactgcgga taatcccttc atttgctaaa gcagcaatgt
    60301 gcgctaaagc ttctccttgc gtttcgattt catagttata gtctgtttta gcgaacatat
    60361 actcccagtc gaagctgata gattggtttt tccaaacacc tatatctaac ggctctttga
    60421 tccctacgat cgttcccaca tgtccaaatg gtcggacaag gtgcttgatt tgatcaaagt
    60481 agcgggtaat atcgaataat acagcgacat actctacttg atcgattcct aattcacgaa
    60541 aacttttact taagtcgtga tgataatcga ttggatagtc tacacctgtt tttttcagcc
    60601 attcaaaatt cttcggactt gcagtcgcgt agacttctag gccagcccaa tgcgctagtt
    60661 gattcaaaat cgaaccgacg cccccggatc cgttgatcac taagattttt ttcccgcgat
    60721 tggcattttc ttctggagtt agaccaaatt tttcaaataa caattcataa gcggtcaatg
    60781 aggtcaacgg caaggctgca gcttcttcat ctgaaagatt ttttggtgca agagcgacaa
    60841 tacgttcgtc aactagttgg tattcttgat tgcttcctgc tcttgttgtt gtacctgcgt
    60901 aaaaaacgcg atcgccgaca gaaaattttt gcacttcgtc acctacagca acaactttcc
    60961 cgactccatc aaaacctaag atacgtaaag catttcttat cccgttatct tggcgaagtt
    61021 tcgtatcgac aggattaaca gatactgcgt tgacttttac cagaagatcc cgtgaactgg
    61081 gtacaggtat ctgttctttt tcatcaataa agctttttgg atcatcgatc ggcaaccctt
    61141 cataaaaacc gactaccttc attaattcct tttctgacat acgaattcct ccttagcttc
    61201 atcaagttgg agcatccata cagtgttcat ttcaatatat atagtatgct atttgttcca
    61261 ccttttttat tcatcataat tattctctta cttaaagtaa actatttgca cttttttggt
    61321 taaaaaagag gttgcgacaa gacttttgtc acaacctctt ataaacggtg ttctcctacc
    61381 aactcttcgg tcacaagtca aaatattcat caaacatgag aagctgtttt ttgaaattgc
    61441 tccttgtgac tcagagttaa acggcaggct cacaacctct gatataaacg gtgttcaatc
    61501 aactgaccct cggtattagc tcaaaaaacg acaaaatatg aagagctatt tttgtttggc
    61561 tgttcttatt acttgataca ggacagcttt ttcacgacct ctaattcacg gtgttcaagc
    61621 aagaaatcat tgttcactga aaagcgttgg atgttctttt tcaaaggtct ctaagatctc
    61681 tcgtacttca tcagtagatt tcgtattcat caactgaact cgcaagtcat ttgctcctgg
    61741 aaatcctttg atatagattt taaagaaacg atgtagtcct gtgatggaac gcgggatgat
    61801 ttctgaataa tgatcttgca gatctagttg cagacgaagt agtccgatca gttctttttc
    61861 tgtatgttcc ctcgggtctt tttcaaatgc ataaggattc ttgaaaatcc ctcggccaat
    61921 catgatgcca tcaacgccat atttctcggc caattcttct cctgtctggc gatcggggat
    61981 atctccattg atcgtgatta gtgtttgtgg agcgatttgg tcacgtaaag caatgatttt
    62041 aggaatgact tcccaatgtg catctacttt actcatctct ttacgcgtac gcaaatggac
    62101 agaaagattg gctatatctt gatgaagtag gtgactgatc cattcttcca tctcagacat
    62161 ttccgtatag ccgatacgcg tcttgacgct gacaggcaaa ccgccagctt tagctgcttc
    62221 aatcaattgt gccgctactt ctggacgaag gatcaagcca ctgccttttc cacgttctgc
    62281 tacattaggg actgggcagc ccatgttgat atcgattcct tggaagccca tttctgccat
    62341 agcaatgctc atttctctaa aaaattctgg cttgtctccc caaatatggg caaccatcgg
    62401 ctgttcatct tctgtaaatg ttaatcttcc tcgtacactg tcttttccat ctggatgaca
    62461 gaaactgtct gagtttgtaa actcagtaaa gaagacatcc ggtgctgctg catgctttac
    62521 gacatggcga aatacaacat ctgttacatc ttccattggt gctaaaacaa aaaaaggctt
    62581 tggcagctct ttccaaaaat tagtagtcat ttatttgtta ctctccattc tatcaagcag
    62641 tgaagtcgca atctttctca aaaacattgt cattatacta ggaaacaaaa aaagaagcaa
    62701 agtgaacagc ttaaaagatg tttctgattg agcaaaaaaa agactgtgac aaaaatctta
    62761 tcacagtcca ttttccggaa taaatggtgt tcaaaagctt gcatttgcgg taataagtcc
    62821 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcaga
    62881 acagcttttt cacaacctct ccactaaacg gtgttcaatc agctgaccct cggtattcgc
    62941 tcaaaaaaca acaaaatatg aggaactatt ttcccgtttg tttctctaat actcaggtca
    63001 aaacgctgat ttcacaacct cttttctaac ttctcaatcc tcttccttta ttgatcaaat
    63061 accaagtaac ggcgtacaaa acaacgatga ataacacaag tacgatcatc gatgttgcta
    63121 ctggcacatc gatcgttcca aggaatccgt aacggaaacc ggagatcata taaacgatcg
    63181 gattgacttt tgaaacagct tgccaaaagg gcggtaacat agagatggca tagaaaacgc
    63241 cacctagata agttaacggc tgcaagacaa atgtaggcac gatcgataca tcatcataag
    63301 actgagcaaa aatcccgttg agcagtcctg ctaatgaaaa caagattgcc gtcatcaata
    63361 atgtaatgat gaccatgaac catgaataca catgaagtgg tacaaagaac aaggaaatga
    63421 tcgttacgag agagccgacg agaatactgc gacccacacc accaatcaca aagccccaaa
    63481 taatcacatg cgtaggaaca ggtgctacca agatttcttc gatatttttc tgaaattttt
    63541 gagagaagaa ggacgaagaa acattggcat acgaactagt gatagcagac atcataatca
    63601 aaccaggaac gatgaattcc atatatgaaa aaccacccat gtcaccgatc cgcccaccga
    63661 tcatttttcc gaaaataaca aaatacaagg atgtcgtgat cactggcgga accagtgttt
    63721 ggacccatat acgcaaatag cggttcgttt ccttgatagc taaacttttt aatgcagtaa
    63781 aataaagatt aaacattttt ctcctccgta tgattatttt cttctgtgat tttcaagaac
    63841 agttcttcta aacgattcga cttattcctc atagaaagaa ctttgattcc ttgtcttgta
    63901 agctgatcga agattccgtt caccccttga tttcgttcaa cttcgacagc gattgtctgc
    63961 tcatcttcaa atgtacaagc atatccttca atcacaggtt tggtatcata tggcgctaaa
    64021 tcaaagataa atgtttcaaa ttgcagtttt gacagaagct ctttcatact tgtattctcg
    64081 atcagttcac ccgattggat gattccgatg ttgcgacaaa gcatttcagc ttcttctaga
    64141 taatgcgtcg tcaaaatgat cgttgtacca ttttcgttca attctcgtag aaactcccac
    64201 atctcgcgtc tcaactcgat gtcaactcct gctgttggtt catccaagat cagcaaacgt
    64261 ggttcatgca tcaatgccct agcaatcatc agtcttcgct tcattccccc ggaaagcata
    64321 cgtgcacgga catttctctt ttcccaaaga ttggactgct tcaaatattt ttcgctgcgt
    64381 ttcatcgctt ctttgcgaga gacaccgtag tagcctgctt gattcacgac gatttgctgg
    64441 accgtttcaa aagggttaaa attgaactct tgtggaacca atccaatctg ttgctttgct
    64501 cgaactagat ccttgtccag atcataaccg aagacattca cttttccaga tgttttattg
    64561 actaaagaag taataatccc gatcgttgta gacttcccag ctccatttgg ccctaatagc
    64621 gcatagaaat ctccttcttt caccgaaaga tcgattcccc gcaatgcttc gaccccactt
    64681 ccatagatct ttttcaaatt atttatttca agcgcatttg ccatttattt cctatctcct
    64741 tatagacatg attcttaatt gtatcatgga atacagcaga tgaaaaaaca acttggcttc
    64801 cacacaaaaa cacacctgct aacaaagagg agatgtgttt tctagttcat gatcatcttc
    64861 gatagaataa ctatctaaac aactctggaa cttttaaaat cataaaagta ccgacagcaa
    64921 ctactacgat gacatataga agatccttat aactgaggtt cttgtctttt ttattcatgt
    64981 taagctttcc tccgtccttt tatcgaaagc ttttttcttt tattttaacc gaaataaata
    65041 aaatggaaat agttattttc aatttctgtt ttcgctgttt gttttacgga gaactttgcc
    65101 atggaacgtg cgtctcgtcg ctgatgctaa gttttttcga taaaagctgc tagaaggaag
    65161 acagcgtggt ttattttgta tcgtcttttg ctaaatcttc caagatcatc tggacagcag
    65221 actcgttccc attgaattct gccacttttt tggcatcaat gtacttcttt tgataatcgt
    65281 ctttttggac ccacgcatca tgcttcgttt gataaagtta atcaaacgga taccactgac
    65341 gctgttcatc gaacaacaaa ctttcttttt gggcctgttc aactatggat ttaggataat
    65401 tcaaaacttt cagcaaggca atcgcatttc gggtccttga aggtccttct tttaatttga
    65461 aatcaaaact gattcctttt ccttctgtta cttgttcttc aaagtggaca ttctcacaat
    65521 aattctttaa aatctctgtc aattcgatat catgtgtggc gacaaatgct aaagatggat
    65581 actccgcaag ccaccgaacg actgaagcgg aagaagcaat ccgttcgatc gtatttgttc
    65641 cttttaaaat ctcatctacg aagcagtagc agcgttcttt ggaagccact tggtccagca
    65701 aacgtttgat cgatttgatt tctgaaacaa aataactgtc gccttcaaat aaatcatctt
    65761 cgattgccat ggaagtcaac acatgtccag gttgcagggt aaactgttct gcaatcgctg
    65821 tttggatcgt ctgtgctaga atacagctga tggctatact tttgacatac gtagattttc
    65881 ctgatgcatt ggaacccgtg actaatgtat tcttctgcca gttgactgga ttaactaccg
    65941 cttcttttaa caatggatga tagatatctt tcgctgtcac accgccctca gtaaactctg
    66001 gtcggcaagt gatcggcata taggtgcgat aattcaaaac ggctgctgca acttccaatt
    66061 ctcccagcaa ttcccacagc ttgatggctt cagttgaatg cttttctaat ttagtgagaa
    66121 cgaaatgata ggagataaat ggcagcatga acatgatgtt gacatattca aaaatcaatt
    66181 ctgcttcact cccgctcttc attcgaaatg aaaaacctaa tttagggatg tctcttaatg
    66241 gtttagtttg ctgtttcagc tctttttgaa gtggtgtcgt catttttgct aactgattgg
    66301 cggctgaaat cgttgaaacc aaataactca tgctattcag ctctgtttct agtgtttgtt
    66361 tcttcactag ataatagacc gtattgaata ctaagccacc gattgctaga tagattccta
    66421 gtacttgccc taaaagaagc aaacctacac ccaccagcgg aagcaagcca agaaaaataa
    66481 ataaacctac gcgtccaatc tttttacttt ttccatccgc tagatattgt tttgcaaaat
    66541 tatgatcttt ttttcctaaa cgagcgaatt gatattggat tctttctctc agttgaggat
    66601 tttcctgata aaaagcaatt agtttttcta actgataatc ttctccaaaa tcaaagctac
    66661 gtaaacgctg atacaacgct tctgaaccta cactagaata ggtactgttg atcccttcaa
    66721 atacttcgaa catatccaag tcataccaag taagatcgtc gatttcacta tcccatgaac
    66781 ggaatttttt ctccgtgagc cacgcatcct tcaaactttc ttctttatca aatctcggtt
    66841 gataaggaat ttttccccaa ttactacgaa ctgtttcctt tactttcaaa cgtgaaaaaa
    66901 tgtctaaaac aactaataaa ataacaactg caataataat taaaacgata aattctcttt
    66961 ggcccatatc cctctccgtt ccttgatttg ttagttttag tatagcactg ttgcgctatt
    67021 tgaaaagggg gtacaaaaat aactgtcttc gttacctaat tcgtatcaat cacaccaaaa
    67081 taaaaacacc ttccaaaaaa gaagatgtct ttaaaacgag atacaatttt ttgtcattca
    67141 cttcaggtaa attagagcca attaaaccct ccgcaacttg acggagatgg agaagatgcc
    67201 cccgtctaat ctagcggtca tctctcctcc cattccttcc accaattttt ttgacaggta
    67261 aagcccgatt ccgctagatt ccgtatttgt gcggctggta ttttcggtat aaaaacgctg
    67321 gaagactttt tcaacagagg tttgcgctcc tggtttgata ggattggcca ctgttaatcg
    67381 aatgaatgaa tcctcttttg aaaatgtcac ctttgcttgt ccatctgcat aacgcaataa
    67441 attctgtatg atattttgca gcactcgcag caacatgagc ggatctgcta atacagttgt
    67501 atttatcgag tgttcagcga acgctacctc gatatttttc tcagaaaatt gttcataaaa
    67561 agagaaaaat acttcttcca cctctttaga aaaagaaagc gtatggagat ccatttcttt
    67621 ttgcttcgtc tctattcgag caagatcata aaactgatcc gtcatactga gtaatcttgc
    67681 aacagattgt ttgatgatcg ttaaatactt ctcccgtttc tccgtatcct ctacctgttc
    67741 cagtaattgt acataaccgt taatagcagt caaaggagtt ctgagatcat gagaaaggcc
    67801 gacgatcgat agtctgatgt tttcttccat ttcttgaaat gcttgtcctt tttgttcatt
    67861 ttcatccacc catgtattca gtgcatctac taggtccatt agattctttt ctcgaaattc
    67921 gactgaaaga cgaccaccat attcttgtcg ttcagaaaga gtggcgattt tcttagttaa
    67981 tctcttgatt tccctttgca ggaagaaaat aaaacttccc ataaagataa caacgagaag
    68041 caacagccac ttaatcaaat ctaaacccca ctccccacac cgtatgaata tacacagcat
    68101 gtgcctttgc tagtttccct cgaagattgg aaatatgcat attcactgtt ttctcactat
    68161 caaaatatgg ttcttgccaa actgtttcat aaagatttgc tcgcgaaaat acttttttcg
    68221 gatgttctaa aaaaagcttc agcagatcga attctcttcc tgtcaaatgg acagccacac
    68281 cattcacatt tacatccctc gactctttgt ccagtaagat ttccttgtaa tagatgcgtt
    68341 gcgacagatc ttttacttgg acagaagtct gatgacgaag ttggatgccg attcgtaaca
    68401 acagttcttc gatatcaaat ggtttcgtca catagtcatc cgctcctgct gccaacaagg
    68461 aaactttatc tctttgatcg ctttttgctg aaagaacgat gatcggaaca ttcgatgtct
    68521 gtcggatttc tgctaacaat gcttcacctg aaagccctgg aagcatgaga tctagaagga
    68581 tcaaatcgac aggatatgtg cttaacaata attttccttc cgtacctgaa taagcatcaa
    68641 gaagtttgta tctctgttca agaacctcct ccagtaaaga atgaacagct tcatcgtctt
    68701 cgataatcaa aatagtttcc agcaggaaca cctacttaat gtcagatttt tggaaaataa
    68761 acattcctag agctgatgat accacgattg ttactagact gaccagaatc attttcggtg
    68821 cttgcgaata agttgcttgt tgcgcaaagt ccgtatacat gagccacgtt tccgtaaacc
    68881 actccgttag aaattccaag cctttgattt ttgcgaaaac accgggaacg aaggccgttc
    68941 ccatgatata aagaaaagaa agaagcaaac tgacactggt acttctagtg atgaatgtca
    69001 aagccactgg aacagataat gtcgctaata tcagcagaag caatacagga aatactttta
    69061 gaacataatt tccatagttt ttgattccct caacttcacc aaatccgaaa gcaagtgtgt
    69121 aagcaatcat ggctagcccc gtaatcaaaa ccacgccaag tgttgtgatc aaaagcgtaa
    69181 cgagaagttt cgacacataa taagtcattc gattccgtcc taaagataac gtattgcgaa
    69241 tcgtaccgtt ttgaaattct tccccaatat aaaagcacgc aaatgctgcg aaagccagaa
    69301 gaatccatgc agccattttt tccaccatta aaatccctgt tgcaccgttg atgactgtat
    69361 ccgttgcttg gtttaattca ttgccgtatt ttgtatgcat ataccattcg ttccaagaat
    69421 aaaaaagcgg aagtacgacc atcagtccta gtgagaccca cattttacgg ctcttccata
    69481 cttttatttt ttcagcagtt aatagattaa acatgtggat tgcctccagt taattgtaag
    69541 aaataatctt ccagtttttg cccaactagg tggataccat ccacaggaac ttttgcctct
    69601 accaatgctt gattgatggc tgctacttgg tcaattcctt caaaaagctg gataacacgg
    69661 ctagattgga caaaataatc cctataacca agagagtcga gaaccgttac tgcttgttcc
    69721 gtcgccgaag tgtcaatttt tataaattga cgagattctt gtgccaattc ttctttcgat
    69781 aattctttga tcaagtgtcc ttcatgcaaa ataccgtagt gagtggcgat ctgtgacaat
    69841 tcatctagca agtggctaga aagcaagacg gtgatgcccc gttcttttac caaacgttgg
    69901 atgatttctc gcatttctgc catccctgct ggatccatcc cattcgtcgg ttcatccaaa
    69961 atcaaaaatt ctggattcgt gatcagtgca tttgcaatag ctaaacgctg gcgcatacct
    70021 aaagagaaat tctttgcttt ctttttccct gtattttcca atcgcatcat tttcagcagg
    70081 tcttcgattt ctctatccga cacaccgccg ttcgctgctt ggatacgtag gttatctctt
    70141 gcagttaatt ctggataaag agcaggggtt tcaatcgatt gacccatacg tcgtcttgct
    70201 gcctgcaaat ccgtcgttcc aaataattcg atcgaacctt catcaatcga gatcaaaccc
    70261 ataatagttc tcataaaggt agacttaccc gctccattct ccccaatcaa accatagatc
    70321 attccacgtt tgatttcgat cgatacttta tctaatgctt tggcagcacc gtattttttg
    70381 gttataccag ttgctttcaa gaccgtttca tttccaaaat ccatacttat ccttctttct
    70441 tattgataag tgaagtatag ttgacgggta taaagaaagc agccagaaat gtataaagaa
    70501 agtgtaaatt ttccaattga atttcaagaa aaatcagact gtctcgcaga cgtggttgag
    70561 ttgtctgcca atcaaagagc gcaccaaaca gtgctaatcc aagtggactg gccaccatgg
    70621 taatcgttgt tatcgttgac atcagacgtc cttgtatatg cgttggaact tctcgctgaa
    70681 gccagaccat ataaaaagca aaagaaaaca ttaatcctta aaaacggact tatgaaaaat
    70741 aatacatgcc gtttgggatt tacaattagc taccaaataa gaggttatga caaaagactt
    70801 ttcacaatcc cttcttccga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
    70861 caatatttat caaacatgag aagcaatttt tgtaaattcc ttcttatttc ttgaagctga
    70921 ccacttctgt cacaacctca gaagcggata aataatattg tttgatgata ctttatcttt
    70981 ttcccgtttg tttatagatc gaatgatatt atagaccaag aatcctacct attaacatag
    71041 ctaaaaacac aaaaaaggca ctttccaaat tgtggaaagt gccagaaaag ttaggattaa
    71101 ggtaactgcg ctcagcattg cttcgcccag tacgattctc gtaactcgtg aactcgaacg
    71161 ataatcgcaa ttaacgtttt gagaactgtg aagctttacg ggctttctta agacctggtt
    71221 aaacaaaact ctattttatt gcattttaca ggaatgtatt tcatgcgaaa ttaataagtc
    71281 tttgtttata gagttttact agggtataaa aagttgtatg ttatcattac ctcaaaacat
    71341 tacctcagct tggcgagttg gttgattgaa gaataaatca tttatctaga tatctcaata
    71401 aaaaatgatt tctaagaaac ttgtattttc gtgttattat ttttttgccc tatcaaaatt
    71461 tctaattctt ctagcgtaat atgattatga cacataatcg attcaattac ttcaatacct
    71521 atttttttcg agaaagatcc cttttgttca ctcacaaaaa tactttcaga agacatatat
    71581 aaggaacgaa gttttggaat tagtatatcg tacaaataga ccaccataaa atcgtccttg
    71641 caaccattct caaaaagaat taaatttgct gattgtgtta catatatcac tgaattacat
    71701 tcatggtcaa ttccaaaaac aatgtctact tcattttctt ttctaattcg cgcagttaca
    71761 tttttcaaac tttcaccatt acttataaat gctttagtta ctgtaagctc tgatgtttgt
    71821 tcagatattc cttttataat attttgttca tcagccacat tccccataaa tccgccgatt
    71881 ccttttaagt aataattctc acctaagagt cgcttgcatt cactgaattg ataaaaaaac
    71941 cagttaaaaa aatcttctgg aaaaataaat tctgatcgaa ctttatttat atttttagct
    72001 cctattaatt ttataactcg attaatgttc acttgctgcg atgaacatat aactgaataa
    72061 attgagttgt ttaattcaaa aaaacaactt taacatatgc atcattaatt cgttattttt
    72121 taggtagata ttgatcattg ttatctctct ttttttgctt cccatactcc aacttagaaa
    72181 ttgccgttat aaaatttatt ttaaaggtga ctttttcccc tgaagtatcg gtagtaggaa
    72241 tacttaatac ctcagcttct gaggaggtat cccaattata gttatacttt gcatcatcac
    72301 cataaagaac aattccattt tttttatctt gtgccgttag ctttactggt tcgcttactt
    72361 ttcttgttaa tatcttggac tttacatcat cgatattgct tattttatca agcttaaata
    72421 atttacaaat taatttcgta ctagttttta tatttgacga gagtgtaaaa tattttgtgt
    72481 aaatagaaaa aaggaagtcc cttctgtaga atagagttac cacaacacat tcacagaaaa
    72541 gaggacttcc atatgaacga ttttactaca gaaattctaa agactctagc gaacaaaggc
    72601 gatttgaatg aattattccg tgtccatttg gaaaaagctg tcaatacgct tctcaaaacg
    72661 gagttaacgg ctttcctcga ttacgaaaag tacgatcgca ttggttttaa cacggggaat
    72721 tctcgtaacg gctcctatga ccgtacggtc aagaccgagt acggggaact tcatctccag
    72781 attccgcgcg accgcaacgg cgagttcaag caacagactg ttcctgctta tagacggacg
    72841 aatgatatct tagaggagac cgtcattcac ctcttccgaa aaggtattac catgtcggaa
    72901 atcgcagact tgattgagaa aat
//
LOCUS       DACCCS010000006        65341 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.006, whole genome shotgun sequence.
ACCESSION   DACCCS010000006 DACCCS010000000
VERSION     DACCCS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.006"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(290..1087)
                     /locus_tag="IVK82_000409"
     CDS             complement(290..1087)
                     /locus_tag="IVK82_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP6391496.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1084..2268)
                     /locus_tag="IVK82_000410"
     CDS             complement(1084..2268)
                     /locus_tag="IVK82_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP6391497.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2293..3540)
                     /locus_tag="IVK82_000411"
     CDS             complement(2293..3540)
                     /locus_tag="IVK82_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP6391498.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3755..5500
                     /gene="dnaX"
                     /locus_tag="IVK82_000412"
     CDS             3755..5500
                     /gene="dnaX"
                     /locus_tag="IVK82_000412"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP6391499.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5520..5834
                     /locus_tag="IVK82_000413"
     CDS             5520..5834
                     /locus_tag="IVK82_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP6391500.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5859..6455
                     /gene="recR"
                     /locus_tag="IVK82_000414"
     CDS             5859..6455
                     /gene="recR"
                     /locus_tag="IVK82_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP6391501.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6519..7163
                     /locus_tag="IVK82_000415"
     CDS             6519..7163
                     /locus_tag="IVK82_000415"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP6391502.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7178..7507
                     /locus_tag="IVK82_000416"
     CDS             7178..7507
                     /locus_tag="IVK82_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391503.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7521..8459
                     /gene="holB"
                     /locus_tag="IVK82_000417"
     CDS             7521..8459
                     /gene="holB"
                     /locus_tag="IVK82_000417"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP6391504.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8495..9319
                     /locus_tag="IVK82_000418"
     CDS             8495..9319
                     /locus_tag="IVK82_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP6391505.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9312..9659
                     /locus_tag="IVK82_000419"
     CDS             9312..9659
                     /locus_tag="IVK82_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP6391506.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9728..10600
                     /gene="rsmI"
                     /locus_tag="IVK82_000420"
     CDS             9728..10600
                     /gene="rsmI"
                     /locus_tag="IVK82_000420"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP6391507.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10709..11830
                     /gene="dinB"
                     /locus_tag="IVK82_000421"
     CDS             10709..11830
                     /gene="dinB"
                     /locus_tag="IVK82_000421"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP6391508.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="IVK82_000422"
     CDS             complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="IVK82_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP6391509.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="IVK82_000423"
     CDS             complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="IVK82_000423"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP6391510.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15316..16083
                     /locus_tag="IVK82_000424"
     CDS             15316..16083
                     /locus_tag="IVK82_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6391511.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16073..18115
                     /locus_tag="IVK82_000425"
     CDS             16073..18115
                     /locus_tag="IVK82_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6391512.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18396..18545
                     /locus_tag="IVK82_000426"
     CDS             18396..18545
                     /locus_tag="IVK82_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP6391513.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18556..20070
                     /gene="dltA"
                     /locus_tag="IVK82_000427"
     CDS             18556..20070
                     /gene="dltA"
                     /locus_tag="IVK82_000427"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP6391514.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            20067..21272
                     /gene="dltB"
                     /locus_tag="IVK82_000428"
     CDS             20067..21272
                     /gene="dltB"
                     /locus_tag="IVK82_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP6391515.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21317..21550
                     /gene="dltC"
                     /locus_tag="IVK82_000429"
     CDS             21317..21550
                     /gene="dltC"
                     /locus_tag="IVK82_000429"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP6391516.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21553..22821
                     /gene="dltD"
                     /locus_tag="IVK82_000430"
     CDS             21553..22821
                     /gene="dltD"
                     /locus_tag="IVK82_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP6391517.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22962..24044
                     /locus_tag="IVK82_000431"
     CDS             22962..24044
                     /locus_tag="IVK82_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP6391518.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            24044..24847
                     /locus_tag="IVK82_000432"
     CDS             24044..24847
                     /locus_tag="IVK82_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP6391519.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24917..25657
                     /locus_tag="IVK82_000433"
     CDS             24917..25657
                     /locus_tag="IVK82_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6391520.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25668..26672
                     /locus_tag="IVK82_000434"
     CDS             25668..26672
                     /locus_tag="IVK82_000434"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP6391521.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26717..28561)
                     /locus_tag="IVK82_000435"
     CDS             complement(26717..28561)
                     /locus_tag="IVK82_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391522.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28773..29291
                     /locus_tag="IVK82_000436"
     CDS             28773..29291
                     /locus_tag="IVK82_000436"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6391523.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            29419..30102
                     /locus_tag="IVK82_000437"
     CDS             29419..30102
                     /locus_tag="IVK82_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP6391524.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30411..30484
                     /locus_tag="IVK82_000438"
     tRNA            30411..30484
                     /locus_tag="IVK82_000438"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30445..30447,aa:Arg,seq:tct)
     gene            30643..31002
                     /locus_tag="IVK82_000439"
     CDS             30643..31002
                     /locus_tag="IVK82_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP6391525.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            31125..32756
                     /locus_tag="IVK82_000440"
     CDS             31125..32756
                     /locus_tag="IVK82_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP6391526.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(32818..33459)
                     /locus_tag="IVK82_000441"
     CDS             complement(32818..33459)
                     /locus_tag="IVK82_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP6391527.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            33741..34049
                     /gene="rplU"
                     /locus_tag="IVK82_000442"
     CDS             33741..34049
                     /gene="rplU"
                     /locus_tag="IVK82_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP6391528.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            34076..34420
                     /locus_tag="IVK82_000443"
     CDS             34076..34420
                     /locus_tag="IVK82_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP6391529.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            34433..34726
                     /gene="rpmA"
                     /locus_tag="IVK82_000444"
     CDS             34433..34726
                     /gene="rpmA"
                     /locus_tag="IVK82_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP6391530.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(34819..35967)
                     /locus_tag="IVK82_000445"
     CDS             complement(34819..35967)
                     /locus_tag="IVK82_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6391531.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(35979..36290)
                     /locus_tag="IVK82_000446"
     CDS             complement(35979..36290)
                     /locus_tag="IVK82_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391532.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(36343..36738)
                     /locus_tag="IVK82_000447"
     CDS             complement(36343..36738)
                     /locus_tag="IVK82_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP6391533.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(36774..37118)
                     /locus_tag="IVK82_000448"
     CDS             complement(36774..37118)
                     /locus_tag="IVK82_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6391534.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            37419..37640
                     /locus_tag="IVK82_000449"
     CDS             37419..37640
                     /locus_tag="IVK82_000449"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391535.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            37767..38543
                     /locus_tag="IVK82_000450"
     CDS             37767..38543
                     /locus_tag="IVK82_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP6391536.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            38540..38734
                     /locus_tag="IVK82_000451"
     CDS             38540..38734
                     /locus_tag="IVK82_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6391537.1"
                     /translation="MTMSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRR
                     KPDKQIKPIEAALAELEKEK"
     gene            38736..39020
                     /locus_tag="IVK82_000452"
     CDS             38736..39020
                     /locus_tag="IVK82_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391538.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            39041..39229
                     /locus_tag="IVK82_000453"
     CDS             39041..39229
                     /locus_tag="IVK82_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391539.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            39555..39794
                     /locus_tag="IVK82_000454"
     CDS             39555..39794
                     /locus_tag="IVK82_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391540.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            39800..40486
                     /locus_tag="IVK82_000455"
     CDS             39800..40486
                     /locus_tag="IVK82_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391541.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            40489..41319
                     /locus_tag="IVK82_000456"
     CDS             40489..41319
                     /locus_tag="IVK82_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP6391542.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            41336..42187
                     /locus_tag="IVK82_000457"
     CDS             41336..42187
                     /locus_tag="IVK82_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6391543.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            42184..42543
                     /locus_tag="IVK82_000458"
     CDS             42184..42543
                     /locus_tag="IVK82_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP6391544.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            42558..42719
                     /locus_tag="IVK82_000459"
     CDS             42558..42719
                     /locus_tag="IVK82_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP6391545.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            42716..43021
                     /locus_tag="IVK82_000460"
     CDS             42716..43021
                     /locus_tag="IVK82_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391546.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            43021..43377
                     /locus_tag="IVK82_000461"
     CDS             43021..43377
                     /locus_tag="IVK82_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP6391547.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            43337..43582
                     /locus_tag="IVK82_000462"
     CDS             43337..43582
                     /locus_tag="IVK82_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391548.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            43603..43692
                     /locus_tag="IVK82_000463"
                     /pseudo
     CDS             43603..43692
                     /locus_tag="IVK82_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            43767..44234
                     /locus_tag="IVK82_000464"
     CDS             43767..44234
                     /locus_tag="IVK82_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP6391549.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            44509..44745
                     /locus_tag="IVK82_000465"
     CDS             44509..44745
                     /locus_tag="IVK82_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391550.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            44769..45110
                     /locus_tag="IVK82_000466"
     CDS             44769..45110
                     /locus_tag="IVK82_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391551.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(45103..45282)
                     /locus_tag="IVK82_000467"
     CDS             complement(45103..45282)
                     /locus_tag="IVK82_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAP6391552.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDMTK"
     gene            45504..46151
                     /locus_tag="IVK82_000468"
     CDS             45504..46151
                     /locus_tag="IVK82_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391553.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            46411..46755
                     /locus_tag="IVK82_000469"
     CDS             46411..46755
                     /locus_tag="IVK82_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP6391554.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            46760..47041
                     /locus_tag="IVK82_000470"
     CDS             46760..47041
                     /locus_tag="IVK82_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391555.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            47144..47458
                     /locus_tag="IVK82_000471"
     CDS             47144..47458
                     /locus_tag="IVK82_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP6391556.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            47436..49130
                     /locus_tag="IVK82_000472"
     CDS             47436..49130
                     /locus_tag="IVK82_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP6391557.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            49150..50328
                     /locus_tag="IVK82_000473"
     CDS             49150..50328
                     /locus_tag="IVK82_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP6391558.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            50291..50977
                     /locus_tag="IVK82_000474"
     CDS             50291..50977
                     /locus_tag="IVK82_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP6391559.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            50977..52137
                     /locus_tag="IVK82_000475"
     CDS             50977..52137
                     /locus_tag="IVK82_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP6391560.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            52147..53022
                     /locus_tag="IVK82_000476"
     CDS             52147..53022
                     /locus_tag="IVK82_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391561.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            53019..53330
                     /locus_tag="IVK82_000477"
     CDS             53019..53330
                     /locus_tag="IVK82_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391562.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            53320..53673
                     /locus_tag="IVK82_000478"
     CDS             53320..53673
                     /locus_tag="IVK82_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP6391563.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            53663..54064
                     /locus_tag="IVK82_000479"
     CDS             53663..54064
                     /locus_tag="IVK82_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391564.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            54057..54461
                     /locus_tag="IVK82_000480"
     CDS             54057..54461
                     /locus_tag="IVK82_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391565.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            54473..55078
                     /locus_tag="IVK82_000481"
     CDS             54473..55078
                     /locus_tag="IVK82_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6391566.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            55098..55460
                     /locus_tag="IVK82_000482"
     CDS             55098..55460
                     /locus_tag="IVK82_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391567.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            55463..55645
                     /locus_tag="IVK82_000483"
     CDS             55463..55645
                     /locus_tag="IVK82_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391568.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            55662..59081
                     /locus_tag="IVK82_000484"
     CDS             55662..59081
                     /locus_tag="IVK82_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP6391569.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            59132..59869
                     /locus_tag="IVK82_000485"
     CDS             59132..59869
                     /locus_tag="IVK82_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6391570.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            59879..62170
                     /locus_tag="IVK82_000486"
     CDS             59879..62170
                     /locus_tag="IVK82_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391571.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            62194..64284
                     /locus_tag="IVK82_000487"
     CDS             62194..64284
                     /locus_tag="IVK82_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391572.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTLDNEKLHFTSDGTGSIIMFTRINTPQLTSGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDAPYYLSKVALGENLIKPESQQPVTNSNY
                     LINTYNIKPMVKGKKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTNVWS
                     KTFTAADDSHPTTPQVQIYQVPSTSVGQCTIKWLKLEEGNTRTPNISEYKYRGTGMRD
                     SNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTT
                     AKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFV
                     ALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNS
                     SYGSDYTFAITYTI"
     gene            64447..64893
                     /locus_tag="IVK82_000488"
     CDS             64447..64893
                     /locus_tag="IVK82_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391573.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            64895..65032
                     /locus_tag="IVK82_000489"
     CDS             64895..65032
                     /locus_tag="IVK82_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP6391574.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            65070..>65341
                     /locus_tag="IVK82_000490"
     CDS             65070..>65341
                     /locus_tag="IVK82_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391575.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT        21856 a        10672 c        13376 g        19437 t
ORIGIN      
        1 ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac
       61 ttgtgaccga ggagttggta ggagaacacc gtttaccaag aggttgtgaa aaagctgttc
      121 tgcatcaagt aataagaaga gccaagtaaa aacagtctct tatgttttta acttggctgg
      181 acttatcacc gtagatgtaa gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga
      241 cacgattttt gtcacagccc tttttttgcg aacattcatt tagcaatttt tagaaacttc
      301 tgccgccacc gccaaaagtc ccgccaccgc tagaatgggt agtacttccg ccaccgccgc
      361 ttccaggagg tgggcttttg gggattcttc ttgtcgtgac aaaagaattt gtcagacgat
      421 cggttttttc agttaatttg atactggatt tttctcggaa tggatatttg tagatgcctg
      481 atttcaattg ataacgcgaa acagtcacaa aataaaagac aagactaagg ataagagcca
      541 aaacaacagc aatgatgatt tctactgtcg tcaaaacctt atagcgtgta attttgccag
      601 tttcttcgtc tacacgataa tgtcctcctg gaactccgtc ttccacatat gttgctgatt
      661 tagacaggta agcttttgca gcattgtaat aggcagcatt tgtcatctga tcataaacat
      721 catctaagat cgaatcgatt ctgctatcag tcaaataatc gatcatatta ccagaagtcg
      781 aaatgtagat ttcacgctgt cccatatcta atagcaatac tgagccattt tgatcatttc
      841 cgactgcgtc tcttaagtaa ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag
      901 tcgttacgat caagactcgt cctttgattt tttcattcag cttttctgcc tgttgttcca
      961 aagattggat ttgatctgtt gtgaataacc cagcctcatc atttaccgta gctgtatctg
     1021 catgagtagc caccggccag ccaacactta ctagcaaaag aaagaataaa aaaaattttt
     1081 tcatcatatc aagtaacctc cgatcagaaa gaggatcgct agcccagcga aaatcccaag
     1141 agcggtcagt cccaattttt tctggctgat cggcaggaca ccgctgacct ttcctgtttg
     1201 tccattcatt gcgtagtagt agactttttt acttgagtca ttgcttcgat aagtaacaag
     1261 ccataccggc agtaatacgt aattatttac ttcgtttgtg attgaagcag aagttcgcac
     1321 accggtcaat gttgtatatc catttgcggt atcttttaac agcttctctg aatagtcttt
     1381 taattcagat tgaatctgtg atttgattgc ctcatattcg atatctcttt tttctgcttg
     1441 gaacccggct aaatactggc ttttaaaatc aattgcttta tctaacggga aaggttgcac
     1501 gccttcgacc atcttttgtt gcgtattttt tgataaggca ttttttacta gttctttaaa
     1561 agaaagcttg cctgctcttg aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc
     1621 tatccgccat atgcggataa ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc
     1681 tgtagcccaa taagggaaat acacacctgt caacttatcg atttgatcct tattgaaaaa
     1741 agctttggga acaaaccatt ttttcttggt ccaagcgaga aatctgttga ttgcttcttc
     1801 tttctcgaca gcaaaaggaa gtactttatt tggcaagaat tctccgctta aacgtcccga
     1861 tagtacgacg ggattatggc agtagtaaca ataagtcgca gcagttgtcg catcagtcac
     1921 gatctctgca ccacagttgg gacaaagaaa aagatctatc gctgcggtat tatctgcaga
     1981 ttcgtccgct gtatctgaaa aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt
     2041 catatcggca agctgttctt gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc
     2101 tttgctttct tcgccagcca tacgcgcttc tttttgcttt tgctcatatt ggctgacttc
     2161 ttcttctgta tagatattca ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa
     2221 agtcaacgga ccgccgcaat taggacattt atgtgtaaga acatccatca gctgcacacc
     2281 tttctaaata aattaattaa cggggattcc tttaaacggt ttaccacagt ttggacagaa
     2341 tttagggatg ccgttggaaa gatccacgat ctcattgcat tcactgcatc gcatttgtag
     2401 cttaggctga acgttttcta caggtttggc agctccgcag ttggaacaga attttccgga
     2461 attttctgtc ccgcaaactg gacatgtcca agtatccgca tttccttgtg cagtttgttg
     2521 attttgtttt tctgcttgtt gtttgatttg ttcttggtta ttttgagctg cttgagaaag
     2581 ataagaacca tttgcattca ttcccatacc tacacccata aagccggtca ttgctcctgc
     2641 ttcatttttt ccggctgctt ccataccacg tgcaatggaa ccttgcacgt atccttcacg
     2701 tacgctagga tcacctagca tcgccccttc gttacgcata ttgatcagtt tcacagaatc
     2761 atcggtgtaa gaaatgctgg ctacagccac agaaacaatc tccatcccac gtaattcacg
     2821 ccaagaatca tccaatgcag tatccatgta tttgcttaat tccaaactct tagatggtac
     2881 ataagaaatc cgttgaccat ctgcagacat ctgattgatt gctgattgta gcgctgttag
     2941 gaattcagct aaatactgct cattgatatc attgatttcc acttgtgttt tgtttttagg
     3001 gattgcgttt gtataaaata aaatcgggtc aacgacatga atagaataag tgccatgtgc
     3061 ccgtaagaat aattctgcgt tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc
     3121 gaatttgatt ccctttattt cttggagatt gatataaaaa acttgttgtt tctgcggtgt
     3181 gacgccgcca aatttgaatc ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt
     3241 gaacatggaa ggagctgcgt catttttaat ggtgtaataa ccttcctcag cggtataatc
     3301 gatgattttt ccaccatcga ccaaaagcat catcatgttt ggatagacat ggacgactgt
     3361 tccgtctgtt aaaacatctt ctgttccttt acgattggag ccacgtttgt catctttgcg
     3421 gactttgaca ccttttgtca tgaccgttgt atcactcata ttgtctggtt cgatgacttc
     3481 tagccattga tctgccagac cgccgccaac catacttgtt gctgctttga taagacccat
     3541 aaaaaattcc tcctctgatt cagctagtgc caaacaatta ttgtttattt attataccat
     3601 aggaagatgt gaaacttctc ggactagaga cggaattgaa atggaaaatt gcgtattgtt
     3661 taaatacctg aaaaagccga gaaggatagt agttttttta cctaatatca cgtataataa
     3721 gagatagtga attttttaaa aaagggggat ccttatggca tatcaagcac tttatcgagt
     3781 ctggcgttcg cagcgtttcg aagacatcgt tggacaaaaa gctgtgacac agacactaaa
     3841 aaatgctatt gtttcccaca aaacctcaca tgcctacttg tttacaggac ccagaggaac
     3901 cggaaagacg agtgcagcaa agattttcgc aaaagcaatc aactgtccga acagtaaaga
     3961 cggtgagcca tgcaacgaat gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga
     4021 tgtgatcgaa atcgatgctg ccagcaataa cggagtcgaa gaaatccgat ttatccgtga
     4081 tcgggcaaat tatgcgccta caaaggcagc gtacaaagtg tacattattg atgaggtaca
     4141 tatgctttca acaggagctt tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag
     4201 tgtcatattc atcttagcaa cgaccgaacc tcacaaaata ccagctacga tcatttcacg
     4261 gactcagcga tttgatttta aacggatcaa tacggcagat attgtcgaac atcttgaatt
     4321 tatcttgaaa gaatcgacta tcagctatga accagctgca ttgaatgtga ttgcccgttc
     4381 tgcagaaggc gggatgcggg atgcattgag tattcttgat caggcaatct cgtttagtga
     4441 aggaacaatt actttagatg atgccatgca agtgacaggt agcttaactg acgaaatgat
     4501 ggacagttac ttgagtgctt gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa
     4561 ccaaattctc gcagccggca aagaatcacg tcgctttcta gaggatatgt tacaatactg
     4621 ccgtgacttg ctgatgtatc agcaagcacc taacctagtt gaggaacaat cgagtacgat
     4681 cagaagtaca tttacagaat tggcacaaac tgtttcagcg gatcagctgt tttcaatgat
     4741 aaagattttg agcgatactc aaaatgaaat tcgttttacc aatagtgccg ctatttattt
     4801 ggaagtcgct acagtcaaac ttgcacagct accgtctgct tctgttcctt cagcagatgt
     4861 agcatttgca gatgatcaga tagtaaaaca gctacaacaa gagattaagg aactaagaaa
     4921 cgaaatcaag gaactacgca gtaatacagg aattgagaat acaaaagtac agccggtaca
     4981 gcaaacaaat cataaaaaac agacaagtac ctatcgtgta ccgacggaac gagtatataa
     5041 agttctgcgg gaagcgacaa gacagcatct gaaccaagta aaagaagtat gggaagactt
     5101 gctgatgagt ctatctgtca ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc
     5161 tggacctaca ggactagtga ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa
     5221 tgacgaagaa ttgcagcttt cggtacacaa caacttaagc cgaatgatca aagattatgc
     5281 gcctgatcct gtttatatta caagagaaag ctggccagaa cttcgaagaa attatttgac
     5341 gcaggcacaa gaagagggaa cagaaaactc ttttgcttct gaagactcag aaggagaaat
     5401 cgagttgctt cctcaagaac aagaagtacc agaagaaaca cttgtagtaa aagcagagga
     5461 actgtttggc tccgaagctg ttacagtggt agatgattaa taaaaaggag tagataatta
     5521 tgatgcgtgg aatgggcaat atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag
     5581 aaatggcaga agcacaagag ctgctaaata aaacagagtt tacaggtaaa gccaccaatg
     5641 aattggtcaa agcggttttt acaggcgatc gtcgcatgaa agatatccaa atcaatgaag
     5701 aagtcgttga tcctgatgat atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg
     5761 atgcgatcgc acaaattgat gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt
     5821 tgccaggatt ttagttttaa actgaaggag taatgaatat gcaatatcca gaaccaattg
     5881 cgaaactgat cgacagctac atgaaacttc caggaatcgg tcagaaaaca gcgacaagac
     5941 tcgcctttta tacgatcgat atgaaagatg aagtagtaaa tgaatttgct aaatctttgt
     6001 taagtgtaaa acgcgatttg catttttgta gtatctgcgg aaacatcaca gaagaagatc
     6061 cctgcgaaat atgtagaaat ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc
     6121 cgaaagatgt aatggcaatg gaaaaaatgc gagaatatca cggactttat catgttctcc
     6181 atggtgtgtt atcaccgatg gaagggacag gtccagaaga catcaatatt tcttctctgt
     6241 tgcagcggct gcatgatgca gatgtaaaag aagtgattat cgcaacaaat gcaacaacag
     6301 agggagaagc aacagctatg tatctctcac gtctgatcaa acccgcaggt atcaaagtga
     6361 ctcgtctagc acatggtctg tctgtaggct ctgatatcga gtatgcagat gaagttactt
     6421 tattgaaagc tgtcgaagga agacgtgagt tataaatttc taattaagtg ttttttcaga
     6481 tacaatataa aagcagcaca agagatgggg gcaacaatgt gaacggacta tttattacaa
     6541 tcgaggggcc tgatggtgca ggaaaaacca gcatcttgaa tgaactttac cctcttttga
     6601 aagaagtagc aaaaacagaa atcattcaaa caagagaacc aggaggcatt ccgattgctg
     6661 aagagatccg agcagtgatc cttgatccaa aaaatgatcg gatggatgaa cgtacggaag
     6721 cactgcttta tgcagcagca agaagacaac atctagtcga aaaggttctg cctgctctgg
     6781 caaaagggaa aatcgtttta tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg
     6841 caggccgacg catcggcgtt accgagattg ctcggttgaa tgagtttgct acagaaggta
     6901 ctacgccgga ttttaccctt tatcttgacg tagattcaga caccggatta agaagaatca
     6961 agaaaaacag acaaaatcaa atcgaccgtt tagattcgga aggtttggaa ttccaccagc
     7021 gtgtacgtca tgcttactta aagctggcag aagagaatcc ggaaagaatc cacaaagttg
     7081 atgcaagaaa aagctttgaa gaagtgctgc aaacaagtta tcatacaatt attgaacagt
     7141 atccgcaatt ttttgaaaac taggaaggtg aacgtttatg aaaattattt tagcgattat
     7201 ccaagacaaa gacagcaacc gtttatcaaa tgaattgatt gatgcaaata tccgtgccac
     7261 aaaactatca tcgacaggcg gcttccttaa ggctggaaac agtacgttta tcgtgggtat
     7321 tgaagatgac cgtgtagaag aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa
     7381 gcaatttgta tctacacctg ttacactgga tatttcgatg gatgggggcg tcccttatcc
     7441 agtagaagta gaagtaggcg gtgccacagt cttcgtctta cctgttgaag gattccatca
     7501 attttaggag gagctgagct atgaatcaag aaataaactt ggctcaaatg caaccgatcg
     7561 tgtatcagca gcttcaacgg agttttgagc atgggcgtat cgctcatgct tatctttttg
     7621 aaggggagaa aggaacagga aaacatgaag taggtatatg gcttgcacag cacctgttct
     7681 gtacccaaat gaaagacaat ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa
     7741 agaaagaaca tccagatgtt ttggttattg aaccggaagg acaaaccatc aaagtcgatc
     7801 agatcagacg tttgcagacg gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt
     7861 ttttgatcaa agaagctgaa aaaatgaata gcagtgcggc aaacagtttg ttgaaattct
     7921 tggaagagcc ccctggagat tttttagcgg tgttagaaac agatgcagta ggaagaattc
     7981 tccctactat tcaatctcgt tgtcaagttc tgcattttca agaactttcg aaagaagcac
     8041 tgatccatcg attgcaagaa gagcagattc cattggaaaa agcaaaactt ttggctttct
     8101 taacaaacag tttaggaaaa gcagttgaaa tctctcaaga tgaatggttt aatgatgcta
     8161 aggacttgat ttatcaatgg ttcgtttact tgcaaaagca agatactcaa gcctttatct
     8221 atgtccaaaa aaaattggta aaatcattta aagaaaaaac acaacagttt acaggattat
     8281 ctgtcttgat gttttattat caggaagcac agcgtcaagc agtagcaaac ggcgaatatg
     8341 gcaaagtgaa acagataaac cagataattg aacggatatt gatcgcagag cagaaactac
     8401 gcagcaatgt cagttttcag gctgttgcgg aacagttcgt tttgcatacg attttttagc
     8461 ttgccgcaaa ggatgatgat caaggaggat ggaaatggta gaagtagtag gtgtccgata
     8521 tagagatgca ggtcatatct attattttgc accaggagac tctgaatatg tttatagtga
     8581 caaggtatta gtcgaatctc agcaatcctt acatattgct actgttgtcc ttcctaaaaa
     8641 agagattgat cccaatgacc ttcctgaagc tatccgtccg atcttgcata aagcgtcaca
     8701 agctgaatta gaaaaagaca aaaaaaacaa aaaagatgct tatgatgcat tgaaaatcgc
     8761 aaaagaaaaa gtgcagttgc gtgagttgga aatgaaaatg gtcaaagccg aatacacatt
     8821 tgatcgaaat aaattgattt tttctttcac agcggacggg cgaattgact ttagagaatt
     8881 ggtaaaagat ctggccgctg tatttcgtac tcgtattgag cttcgacaaa ttggtgtccg
     8941 cgatgaagca aagataatag gcggaattgg tccatgtggg cgtccattat gctgttcttc
     9001 ttttttaggc gatttcgtgc ctgtatcaat caaaatggca aaagatcaaa gtttatcttt
     9061 gaatccaaca aaaatttccg gtttatgcgg acgcttgatg tgctgtctgc aatatgagag
     9121 cgaagaatac gagacagcga aaaaagaact ccctgacttt ggaaaagtaa ttgctacacc
     9181 tgatggtaaa gggaaagtcg tagggttgaa tctgcttagc cgtatcgtta gtattcggct
     9241 gactggacgg gaaaatcctg tcgaatatga atgggatgaa ttgaaagaat ttatccaagt
     9301 aggtgagatg aatggataag agatcattat acgacggatt gaatcaatta gaatcagagt
     9361 tgagaaatac attaacagaa cttgttgaaa tgaagcatgc tttgcatgag atcgtagaaa
     9421 aaaatacaac tttggagatg gaaaatcaac gtctgcggga acacctgcgg gaattgaacc
     9481 aagttgcaca agctccagct gaaaatgtga agcaggaact ttctaagtct cgtatgaatc
     9541 ttgaaaaaat ttatgaagaa ggattccatg tctgctacga tttatatgga tcaagaaggg
     9601 acaatgacga gccttgtgcg ttttgtctag aagtgatcta tcgcgaaagc ggtcgatgac
     9661 caataacaag aggagactgg agcggagaaa tactgctcca gtctttttat taaggagatt
     9721 gtgacagatg tataatcaaa aaagtttcaa gggaaatccg gtatatgggg atttatatct
     9781 tgtaccgacg ccaattggaa atttagaaga tatgagctac agaagtgttc gtatgctgca
     9841 agaatccgat cttatcgcaa gtgaggatac gcgtaataca caaaagttat tgaatcattt
     9901 tgaaatccat acaccccaaa aaagtttaca tgaacataat tataaagaac gtgtccctca
     9961 attgattgct ttgttaaaag aaggaaaaac gattgctcaa gtcagtgacg caggaatgcc
    10021 ttccatcagc gatcctggac atgagctagt tttagcctgc ataaaagaag ggatttctgt
    10081 gatctctatc ccaggaccta cagctgggat gacggctttg attgcttcag ggctagtgcc
    10141 tcaaccattt ttattttatg gcttccttcc acgtaagaaa aaagaacaga aagaaacatt
    10201 gaaaaagcta aaagaacaga cagttacgct gattttttat gagtcacctt atcggatcac
    10261 tgctacattg acgaatttct tagaaatttt tggaaatcgc caagtcgttt tgtgtcggga
    10321 attgacaaag gtccatgaag aataccttag aggaagtacg gaagagctac tcgcttatat
    10381 cgaagaacat ccagtaaagg gtgaatgttg tctgcttgta gaagggaatt tgttcgaaat
    10441 gccagtgacg gaagttgatg aagagttggg aacgttaaag gaacaagtag aagcaaagat
    10501 cgcttcaggc gaaaaaccaa atgctgctat caaagcagta gcgctgaaaa atgggctgaa
    10561 aaaacaagaa gtctaccgtc agtaccatga attagactga gatagtataa gttgtctata
    10621 atgaaagact aatgaggaaa tcagctacta acttttcaaa tctcttgaac gcgaacgaat
    10681 gttcttgtat aatgaataag aggtgaggat gatggagctg caatttcctt tacataatga
    10741 tacatcaaga aaaatcattc atattgatat ggatgctttt ttcgcttcgg ttgaagaacg
    10801 agatcatcct gagtttcgcg gacatccatt agtaattgcc agacacccca gtgacactgg
    10861 tgggcgtgga gtagtgacta cagcaaatta tgaagcaaga aaattcggaa ttcattcagc
    10921 aatgagtgca caaaaagcgt atgagctttg tccgcaagcc atctttaagc ctggcgacca
    10981 tcataaatac agcgatattt ccaaacaagt acgggaagta ttctatcgtt atacagatat
    11041 tgtggaacca gttagtatcg atgaagcgta tcttgatgtg accgaaaata aaatcaattg
    11101 caagtcagcc atcaaaatcg cccgaatgat ccaagcagat atttgggaga gtgtccagct
    11161 aacatgttct gcgggagtaa gttacaataa gtttttggcc aaattagcat cagactatca
    11221 aaaacctaga ggaatcacgg tagttgcccc tcaagacgct gtgccatttt taaaagcttt
    11281 gccaattgaa aaatttcatg gggtagggaa gaaaactgta cctagaatgc atgagctagg
    11341 gatttataca ggaaaagacc tatatgaatg cacagaaatg atgttgattc gaaattttgg
    11401 aaaaatgggc tattctcttt atcgtaaagt cagaggaatc catgattctc cagtaaacgt
    11461 tacaagagag cgaaaatctg tgggaaaaga gcatacttat gggcagccgt tgcaaacgga
    11521 agaagcagtg ctgactcagt tgcgccaact agctgaaaaa gtagaagaag cactgagaag
    11581 agtccaaaaa catgggaaaa cagtagtttt gaaggtcagg tacacggact attctactgt
    11641 gaccaaaaga gtgacattgc cagaatatat ctataaaaaa gaagcactct tttatcaagc
    11701 aagccttata tgggaggaaa tcttgggagt agaaaaagga atccgattgt tagggattac
    11761 tttgacgaat ctggacccga tgacttatga aaacatcgtc cttccattat gggaaaatca
    11821 agaaatataa aaacagcttt gagagttgaa accggaaatc cgttttctct cccaaagctg
    11881 tttttatatc aagtcttgct ttttgatttg ttcataggtt tctgttgcat ccgtacattt
    11941 ttcccaaatc acaacttgat tggattctaa agatttttgg acgtcaatga tccgctggtt
    12001 gctacttccg cgaaattgta gttttaggtc acgtttgctt aattcaaagc gtccgtcaac
    12061 aaggatatcg atttgcgaaa ggagttctaa tttatcttct gaatcttgta gcaattcatc
    12121 aaaggtatag cctgtccatg accagatatc ttttgtcgta ccgaactctt ttcggatacg
    12181 tcgtacgact tgcagacaaa catctgtatt caaaaaaggt tcgccaccaa gtaaagttaa
    12241 tccttgcacg taatcgttgc gcaagtcagc gatgatttgt tcttctaatt caggtgtgaa
    12301 tggtttgcca tatcggaaat tttgtgcagc tttgttgaag cagccttcgc aggcaaataa
    12361 gcagccacta acgtaaagac tgttgcggac accttcacca tcgacaaaat taaacggttt
    12421 gtagtcagca atatagtttt ggctgtagtc ttttgctaac cattcttttg gttttggatt
    12481 tctcataaaa aaacttcttt catttaaggt tcaaagatat agctattgaa gataaaatct
    12541 actcgaccac ataaataaga acaaactgac ctaaatccat tgttcaagaa agcttgttct
    12601 tattttgata gagggcgttc aaagatacaa agccatcgaa agagggtggt gaaaaaagac
    12661 ttgtcatagc ctcttattct gaataaacgg tgttcaaaag cttgcatctg cagtaataag
    12721 cccaactaaa cgaaaaatat gaggaactat ttttgtttag ctattcttat tacttgatgc
    12781 aggacagctt tttcacaacc tcatttcatg tgtttcacac gtgaggaaat ttctttatgg
    12841 cggccatgta ccatcggacg agcttgcgga tttcctaaat agccacaagt acgtttgact
    12901 acatcacatg tttttggatc atgatttcca cattctggac attcgaaccc tcgttctgtc
    12961 ggtttgaaat caccttcgaa accacattga tagcaatgat cgattggcgt attcgtacca
    13021 agatacccga ttttgtcata tgcaaaatcc catacagctt ctaaagcttt cggattttgt
    13081 tggagcatag ggtattcaca gtagtggatg aatccacctg aacaatattt tggataatcc
    13141 ttctcaaatt ccaatttttc aaatggcgtt gggtttttac gcacatcata gtggaaactg
    13201 tttgtataat attctttatc tgtgatattt tcgatgatgc cgaatttttc tgtatccaaa
    13261 cggcagaaac ggtcagttag actttcactt ggagttgaat agacactgaa atgataacca
    13321 tattcatccc cccatgcatc tgcattagct tttaattctt tgacgatatc aagtgtgaaa
    13381 gcttttgctt ctgggtttgt ttcccattcg ccaccgaaga aagctgaagc aacttcatac
    13441 aatccaatgt agcctaacga tacagtagct cgacggtttt tgaataattc attgacagaa
    13501 tcggtacgtg aaagacgttt cccgaaagca ccatacatat ataaaattgg cgcatttgct
    13561 ggaatggctt ctttacaacg ttctacacga tagagaagcg catctttcat gatttctaag
    13621 cggtcagaca gtaactgcca gaatttattc tgatctcctt ttgattcgat cgcgattctt
    13681 ggcaaattca atgtaacaac acctaaattc attcgtccga catttacttc ttgaccattt
    13741 tcgtctttcc acccttgcaa gaatgaacga cagcccatag gtactttgaa acttccagtt
    13801 aattcaacga ttttatcgta attcagaata tcaggataca ttcttttcgt tgcacattcc
    13861 aatgctagtt gtttgatgtc gtaattagga tcttcagggt ttaagttcac accttttttc
    13921 aaagtgaaga tcagtttagg gaagatagct gtccgctttt cactccctaa tccattgatt
    13981 cgaatctgta aaattgcttt ttgaatttct ctttcaaacc agttttctcc tagtccaaat
    14041 cctaatgaag tgaaaggagt ttgtccattt gaagtaaata aggtattgat ttcatattcc
    14101 aaactttgca tagcatcgaa gatatctttt ttggtttttg cttttgcata ttctttttgg
    14161 cgttctggcg aatcgatcca ttcttccgcg tcttttagat gttttttata gttcaattct
    14221 gcaaaaggag caagcaattc atctgttcgg tcggcagaac agccaccata ttgacttgac
    14281 gctacattgg caataatctg tgagatttgt gctgtggcgg tctggataga ttttggcgat
    14341 tcgacttcgg cattgccgat tttgaaccca ttgttcagca tgcctttgaa atcaatcaag
    14401 cagcaattcg tcatcggtgt ataaggatga taatccaaat catggtaatg gatatcgcct
    14461 ttttgatgcg catttgctat atgtggtggc aacattttca gtccgattga ttttccaacg
    14521 atcccggctg tcagatctcg ctgtgtattg aaaacattac tgtctttatt tgcgttttcg
    14581 ttcacgacag tttggtcctt gttgattagc ttttcaatcg aaaagttgat atctgttgct
    14641 ttactgcgag caaaatctcg tttggtacga taattgatgt actcttctgc taattcatat
    14701 tcgttttttt ctagtaatat atgttccacg atgttttgaa tctcatagat tttgacattt
    14761 tcagcaaacc ggcttttgat ttcttggaca accaagtctg caatcatttg aattttttca
    14821 taagtaagtg gtgataatga gccgtgaatt ttcttttctg ctttgatcag tgcttcattg
    14881 atcttttgtt catcgaaatc aacatggcgg ccatcgcgtt tgatgacttt catctgggat
    14941 gtctctggct gctgagtaac gacagttttt tcttcataat ttactttcat tatcttcaac
    15001 tcctgctaaa ttgttcttgt ctatcataaa acaaaatcgg ggaaggagca actaaatatc
    15061 ggctatatat agtattcaat gtgctgtata attggatttc tatactatat gtagtgtata
    15121 ttttattgtg aaaaaaggtg aaaacttgta gctggtggat ttgttttttg catatttaat
    15181 tttgctgaat aaaataattg aataaaattt tttattcagt ccaagtaaaa gtgacaaaaa
    15241 cataagagca aattattcgt agatatgcta ctatgcaaat aaatagaaat gaaaaatgaa
    15301 agaggaggag aagaattgga aaagatgatc gaagtgaaac atctaagtaa aagctacggc
    15361 agcaaacttt ctcccgtcaa agccttagat aatatctcat tcacagtaga agaaggcgag
    15421 tttgttggga ttatgggccc aagtggtgca ggaaagacaa cgctgatgaa tatcttgtcc
    15481 acgatcaatt tgcctacgat gggcagtgtc tcgatccaag gaaaagaaat cacaaaaatg
    15541 aaaaacagtg agctcagtga ctttcgtcga aaaaaactag gattcatttt tcaagagttc
    15601 aatttgatcg ataccctaaa tgcaaaagac aatatcctat tgcccctagc agttgaacgg
    15661 atgactaaag aggaaatgga aaagagagta agacatgtag ctcaattgtt gaatatagaa
    15721 gaactgctca aacggtatcc cgatgaactt tctgtcggac agagacaacg aattgcagct
    15781 gctagagcac ttgttgttca gccacaagtg atttttgcag atgaaccaac tggctctttg
    15841 gactcgaagt cggctacaga attgttgaac tacttgaaga ccatgaatca aaaagaaaaa
    15901 gcaacgatac tgttagtcac ccatgatcca tatacagcaa gctactgcga tagaatttta
    15961 tttattaaag atggtgtgat tttttctgaa gttgtcagaa gagggacaag aagagagttc
    16021 tttgagaaag taatagacat gcaggcgacg attggcggag gaggaaaaat gaatgctgta
    16081 taaactagcc gttaaaagtt tttttcggca gtcacgaggc tatcttgttt attttttcag
    16141 tttgacttta tccgtaatga tctattattc cttcagcgca atgacctacg atcaatcttt
    16201 agtcagaaga gcaagtcaag atacttcgat agatggtgga ttgagttttg gtggatttat
    16261 cattactgtt gttttgcttt ttttcgtatt cagtgccaac cgatttttct tgaatagaag
    16321 gcaaaaggaa ataggcatct atcagctttt tggtatgaat aaactgcaga tatcaggtat
    16381 atatgtgtta gaaatcatga taattggatt gttcgcctgt atttctggga tattactcgg
    16441 tattattttt tccaaactct tttcaatgat tttggttcga atgatggata tggatcttac
    16501 tagtccattt tttatctcca ttccatcagt agtagatact ttatttgctt ttttcatcat
    16561 ccttttagca gtatctgtat attcaatatg gaaaatatgg cgctatccaa tgattcgaag
    16621 ttttggagaa aaagaacaag cagatagttc aacaatgcgc ttcaggacaa ggcaccgatt
    16681 actcgcagtc atcgggttac tacttttaac gagcggatat tttggagcta gtcattttag
    16741 ggaaatcact tcatggatga tcagttatca ttatcttgat acattagttt tcttttttcc
    16801 ttttttaatt tttttcgttt gtgtggtcgg tacttatttg tttttctgtt atacatttcg
    16861 gttatttttg atcatattga gtaaatggaa gatttattac tatggtatca attttttgat
    16921 aatgggtaac acgcaagttc atttgctcaa aagttggcgt acaaattctc tgattacagt
    16981 cattctcgga atttctttaa tgacaattgg cggcacggct agtatctcta ctattttatc
    17041 ctaccaggag aaagtttcct ccccgatgga ctatcagttt gatgttgaga cagagaaaaa
    17101 aatcaagccg attctagcag aggaagggca gaagataact aaagaaatca ccttgcatta
    17161 taaagtcatt gggagtatct atagtcaaaa tctcgaagaa cctatgtttc aaactgctaa
    17221 tctgatcacg gaaacacaat atcaagcctt cagaaaagtt aatccagatt tgccgaacat
    17281 ttctttgaaa tcacctggtc atactgtttt gcttgatcaa atgcaaactc tttttcggaa
    17341 tatttcatta tatggatcta gtattggact gccagataat caagaattga agattcaaca
    17401 aatgcttcca agtgtattag gtgacgaaac aatggtatat ctaagtcctg tactgattgt
    17461 agatgatacc gtttttgaaa aagcaaaagg tgtggattat caagtagtca atgtagatgt
    17521 atcaggtgga aataatgaaa aaatagatga acgactgagc aatgacctgt cgataaagtg
    17581 gctcaatgct gtttattatt cttatgatat cgtaaatgga agtctacaag gagaaattag
    17641 cacaaagaaa cttccggatg accaaatgaa ggaagcacaa tttagccaag aaagctctcg
    17701 tttgaatttc tcttccagct attcgaaatt gcgtgcagtt aatcggcgaa tcggtatcaa
    17761 tatttttgtc acactatttg tcgggatgat cgtgattatt gctactggta gtattttgac
    17821 agtccggcaa ttagcagaag cagaggaaga aaaagaaaat tatcacctat tgactaagct
    17881 ggggattcca caaagaagga tcagacggat gatcttcgag caaaacgcac ttaccttctt
    17941 tccacccatg atattaggga tcactcacgc agtatttgca atcaatgtct tcactcagta
    18001 tataaaaact gccgattact ggttggctta tcttgtgagt ggcttattga tccttatcta
    18061 tcttttgctt tatgtcatta cgagtcagtt atattgtcgg attatcgagg aatgatatac
    18121 ggaataagac aagataataa atagaactaa attttgaata ccatttattt ggaataagag
    18181 gttgtgacac gatttttgtc acaacctctt tattttttat attttttaaa agtacgagaa
    18241 attataaaga aaaaagcagt gatttgatgt gctattttta taaaagctat gtgacaaaac
    18301 tgttagtaaa aaaacggttt ctgtaagcta gacacatgtt tctttttgtc aaaattagtt
    18361 acactttgtg tgaggttata tgaggaggga atccaatgaa caaatggcgt tctaatccaa
    18421 aattgaatta ttggggaaaa ttcatcattc gaacaatttt ttatagtgca gtactgctgt
    18481 tcctgattta tctgtatcac tataaaaata ttcaaggagg aacttttatc tataacgaat
    18541 tttaggagga agagtatgga aatcaaaacg attattgaag caatcgatga ttggggaata
    18601 aatgaacctg atcgagttgc ttatcaggcg gatgagacgc atacttttgg tgaattgaaa
    18661 caagcatcag acgcacttgc ttattattta gaaaaaaagg tagaaggcga tggaccaatt
    18721 gtcgtttttg gcaatttgga gtttgaaatg attgtctcat ttctaggggt tgtcaaatct
    18781 ggccatgcct atttgccgat tgaagagcat acacccaagg agcgtatcct atcgatcttt
    18841 cgggtggcta aaccatctat ggtcatcagt atcggggact ggatagaaga tattccttcg
    18901 gttcctgtca taacaaaaaa agaatttcaa gaaatcaaga agataccagt aggattttat
    18961 cccagaaata gcgtaaaagg tacagaaaat ttttatatta tttttacttc tggtactact
    19021 ggagaaccaa aaggcgtaca aatcagtcat gataatctag tcagctttgt tcaatggacg
    19081 atggaagact ttgggatcaa gcctggtatg catttcttag cccaagcgcc attttctttt
    19141 gatttatcgg tattttctat ttatcctgca ttagtttctg gcggaatgct gaagccgtta
    19201 aataaagcag ttgttcaaga ctttcgtcaa ttattcgcga cattgccagc cttgaagttg
    19261 aatgtgtggg tttctactcc atcatttatg gatatttgct tgatggaacc tacattcaat
    19321 ggagaaaatg tgccggaatt ggacatgttt ttattttgcg gagaagaatt gacgaaaaaa
    19381 actgcagaaa gtctacttga tcgattccca aatgcacgga tatataatac ttacggtcca
    19441 acagaagcaa ctgtagctat ttcaagtatt cagattgacc agcatgtact agatagttat
    19501 gaccgactac cgattggcta cgtaaaaaaa gacacgaaag tttctattgt ccaagaaggt
    19561 aaacctgttt caaaaggaga aacgggtgaa atcattattg caggaccaag cgtatcaaaa
    19621 ggttacctga acaatcccga aaagacagct gctgcgttct tttgtcaaaa tgacttagca
    19681 agttaccata caggggatgc aggtagacta gatgaatatg ggcttttatt ttatgaagga
    19741 cgtatggatc agcaggtcaa attacatgga taccggattg aactcgggga tatcgaacat
    19801 tatctgctcc aagacaaccg aatcaaacaa gctgtcgtcg taccaaaata ccaaggaaca
    19861 aaagtacagc agctggttgc ttttgtcgta ttagaaaaca aaacgaaaca gccggatttt
    19921 cagttgacta aatctatcaa agaacaatta ctagaagttg taatggatta tatgattcct
    19981 cagaagttcc attatgtcga tctgctgcct cagacagtta atggaaaaat cgatcgaaaa
    20041 aaattaacag ctgaggtgaa tcctgaatga tgttacctca tatgatccct tatgcagagc
    20101 cgttttattt ttttctttta gccattgcgc ttgtaccgat cgttctatct ttattgatat
    20161 gggaaaaaag attaccagtt tatcaatcag cagtgacact tttcttttta tttgtcagct
    20221 ttggaggtaa ttattggcaa cagggagcgg cgctgattgg gtatgttttg tggcagacga
    20281 tacttgtatg gggctatttt gtttatcggc aaaggaaaaa tcagacagga tggttttttg
    20341 ccgccgttat tttagcgata attccactgt ttttaacgaa agtgactcct ttttttgaac
    20401 atggaaaagc atcgttactt ggttttttag gaatctctta tttaaccttt aaatctgtac
    20461 aaatgatcat ggaaataaga gatggcatga tcaaaacata taagccattt cgttatatcc
    20521 agtttctgct gttctttcca acaatctctt ctggaccaat cgatcgctat aggagatttg
    20581 aaaaagatca gctccatccg cctgttcgtg ataaatatgt tgatcttttg ggaaaaggcg
    20641 tccaaaatat ttttgtcggt tttttatata aattcattat cggttattat tttggtcaag
    20701 tactgcttcc tgtagtggga cgcattgcct tacagcatgg tggtatttca tgggcattag
    20761 taggttatat gtacgtgtac agtatgtatt tgttttttga ttttgctggc tatagcttat
    20821 ttgcagtcgg tacaagctac atgatgggat atcagacacc aatcaatttc aataaacctt
    20881 ttttatcatg gaatatcaaa gaattctgga atcgttggca tatgacgctg tccttttggt
    20941 tccgtgatta tgtatatatg cgattgatgt tcttcatgat gaagaaaaaa ttttttaaaa
    21001 gcaagatcgt aatgtcaaat atcggttatt ttgctttgtt tcttctaatg ggtatatggc
    21061 atggattgac gtggtactac atcgtatatg gactgtatca tgcttgtctg atctgtctga
    21121 cagatgcatg gatccgattt aagaagaagc ataaagagca gttgccttct aacaaattta
    21181 cgcatggctt agccgtattt ttgacatttc aagcagtatg ttttagcttc ctgatttttt
    21241 caggattttt agataaactt ttttttagct aacgaatagt gacatattaa tttatataac
    21301 aaaggagaaa aggaaaatgg aagaacaaat tttatcaatc ttagaagaaa taacaggaac
    21361 agatgaagta agaaaaaatc gtgatattga tctatttgat gaagggttgc ttgattcttt
    21421 agcatctgtt caattattag tcgagctaga cggagagctg gatatccaag tccctgtatc
    21481 tgaattcgag cgagaggatt ggagcacacc gaataaaatc attcaacaag caactgcatt
    21541 gaagggttga gtatgaaaaa gaaacttttt gctattttcg gcccggttgt tttagctgtt
    21601 ggcttactgt ttgtgttttt tacctcctcc tatcaagtag atatctctaa tccagaaacg
    21661 attagacgtg cctcttcttc catgtcagtg aacgtgttga aaggagatgc gataaaaaat
    21721 tatgcattat ctgaaaaaca atatattcct ttttttggct caagtgaatt aagcagaatc
    21781 agtccatttc atccgtctgt tctggcagaa aaataccagc gaaactatcg tccatttttg
    21841 ctgggagcac ccgggacaca atcgttaagc caatatatga tgatgcgttc tgcaggagat
    21901 gctatgaaaa acaagaaagt cgtctttatt atttctcctc agtggttcgt taaaaatggt
    21961 gtaaaaactg attactttaa tacgtattat tctgaacttc aaacatacga ctggttgttt
    22021 tcaatgaaga aagtaacgcc tgctgatcgc tatttagcta gaagattatt aactttctca
    22081 aaagtaaaag agaatgatac gttgacggcg attctgcaaa cgatcaaaaa agggaagcta
    22141 cctttacctg aatctttgaa tcagctgcgc agccaatgga acatgctgaa gagagaggat
    22201 gaagtattca gcaatattgg gttgaaagat cgacagcaaa aaatcgatca tgagtcaaaa
    22261 cgattaccaa aacaatacca agaaacagaa ttatccattt tagcaaatca aattggagaa
    22321 cgagaaacga cgaataatcc attcgggttg aaaaacgatt tttatacgca tcgtatccga
    22381 gcccacgagc cagagctcaa acagtcacaa aaaaattggg attatcgttt ttcgccagaa
    22441 ttttctgatt ttcagctggt cttggatcaa ttagcaaaaa accataacga agtattgttc
    22501 atcatcccac cggttaacga gaaatggagc gattataccg gactatctca agaaatgtta
    22561 caaggatttg ccaaaaagat caaatttcaa ttaaatagcc aaggattcaa tcgaatagct
    22621 gattttgtca atcaagcggg taccaactat tttatggaag acacgatcca tctaggctgg
    22681 aaaggctggt tggctgcaga tcaacagatc agaccgtttt tagaagaaaa tcatattact
    22741 gctagtaagt atcatcttga cgatgctttt tttagcaaat cttggcaaca tcaaatacca
    22801 gataaactac aattaaaata aagaaaacag gcgagtgttg ctcgcttgtt ttctttattt
    22861 ttgaatattc tgaataaaag ataaaaatac caagtttgcc tgaacaaaaa tcataagcaa
    22921 tggtataatc acaatatgaa tatagaaatg ggggaaaaca tttggataca aaagaagaac
    22981 agctacttat tgatttaact tctgcaaaag gagtacctgg aaatgaagaa gaggtaagag
    23041 aagtattcag agaatatgcc aaaccattcg ctgatgatat tttttatgac ggtttaggca
    23101 gtgtgattgc caaacatggt gctggcggag gccccaaggt ctttatttct ggacatatgg
    23161 acgaagttgg ttttatggta acaaaaataa ccgaaaaagg attcttggaa ttccaaactc
    23221 ttggaggttg gtgggggcaa gtcatgcttg ctcagcaagt tgaaatcaaa acacgtgaag
    23281 ggaaaatcgt ccacggtgtg attggttcca aaccaccaca tgttttaaca cctcaagtaa
    23341 gaaataaacc ttatgaaata aaggatatgt tcattgatat tggcgcatca agccaagaag
    23401 aagccaaaga gtggggcata cgtcctggag atatggtcac tccatatatt gaatacaaac
    23461 gaatgaatgg cagtaaatat ttactggcaa aagcatggga taatcgtatc gggacagctg
    23521 tttctctgcg agtattagag aatctatcta aagaagcaca cccaaatgtt ttattcgcag
    23581 gaagcgatgt gcaagaagaa gtaggcttgc gaggtgctcg aacaagtaca caccttgtaa
    23641 atcccgatat tgcttttgct ttagatactg gtacagcagg agacactcca ggaatgacac
    23701 caaaagaagc agattcgatt ttaggaaaag gcccacagat tttgattttt gatgcatcta
    23761 tgatcccgca taaaaaatta ttgaattttg ttattgatgt agcagaggaa atggatattc
    23821 cattccaata tacagtgatt tctggcggag gaactgatgc aggacaaatg catttgacaa
    23881 gagatggtgt tccgtctgta gccattaccg ttcctgttcg ttatctgcat tcccatacat
    23941 cagtgattca tgaagatgat tatttgaata cagtgaaact agtcacagaa ttagtgaaac
    24001 ggcttgatag tgaaacagtt gaaaaaattc ggagttatga ataatgaata atagaatacc
    24061 tatttctgaa tatcggaaaa aagcacgagt ttctttggaa aatcaatggg gaatcaatac
    24121 ttggcttgtt ttcttatcta taatatttac gagcgtcatc cagaactttt ttgacggttt
    24181 cagtcctttt ccggaagaaa gcatgcagca gcacttattg agttttctag tcgataattt
    24241 acttttattc gcattcaact atgcattgtt ttacattgcg ctagtagttg tacggggtgg
    24301 cagaccaaaa agtaaccttt tattttcagt tttccaaaga aagtattttg gttcgatact
    24361 tggcatcaat ttgatcaatt cagtcatcaa ctggctactc aacagtttaa tctttctgcc
    24421 catgttttta atcagcggag tgagtactta tatgcagctg cttttcagtt caaatgcggt
    24481 acctacgggt gtcatgccag aagattttct tgatatcagc tttgttttat cgatgatagg
    24541 gatgattttt gtttcagcac tcatcatgag tattgttggc ggtttattcc aatttgctgc
    24601 atggacaaag ttcgactatc ctgatttgtc ggttattcaa tgtttgaaat atggctggtt
    24661 tttattgaaa gatagaattg gtacgtatat tttgttgcag ctgtctttta tcggctggta
    24721 tttgctagga gcaatcgctt tgttcatcgg tcttttgtgg gtcattgttt atgtgaatgt
    24781 gtcagttgca gaattttatg aacaagcacg gattgaaaaa ggaggtccag acgcctattt
    24841 tacttagcat gtaagcagtt tattcatcaa aaaaataact tttgtggtag gataaatctt
    24901 tgagggggaa tgtacgatga aattaactgt tttaggatgc ttaggagcgt atccttataa
    24961 aaatcaaggg acaacaggtt atttgcttca atctgacggg ttcaatttgt tgatcgatgc
    25021 aggtagtgct acgctgatca aattacaaga acaccttgat ccacttgatc tagatgctgt
    25081 gatccttagc cattatcatc atgatcatat tgctgacctt ggtgtattac agtattactg
    25141 gcaacttcat cctaagagaa atccggagac attgctttca atttatggac acacaaaaga
    25201 ttccattcac tttgaagaat tgacgttgga aaatgtctca aaaggacatc cttattttga
    25261 agctgaagaa ttaaaaattg gtccttttct gattactttt atggaaacaa tccatccagt
    25321 agtatgttac gccatgcgta tcgtagaaga aacaacgggg aaagtattcg tatttacagg
    25381 tgattcaggt tatttggaaa gtttcgctga ttttgcgaaa gatgctgatt tgtttctagc
    25441 agatacctat ctttttgaag gaaatgaaca tcatcatgcc catttcactt caaaagaagc
    25501 tggagaactt gcgaaatcag ctaacgtaaa gaaacttgta cttacacacc tacctcagtt
    25561 tggtgattta gaacaattga aaaatgaagc gcaaaaggca gcaggcaaag ggattgatat
    25621 agaacttgct gcagttgaca aagtgtttaa catttaggag gaagaacatg atttttgtac
    25681 caaatgaaaa ccacgaccca agaatcaatt tagcaattga acaatatttg ctgcaagaaa
    25741 tgcctttaga tgaacctatt ttgctctttt atatcaatga accttcaatc atcatcggca
    25801 gaaaccaaaa tacgcttgaa gaaatcaatc gagattatgt ggaagaaaat ggcattcatg
    25861 ttgtccgtcg cttaagtgga ggcggagctg tctaccatga ttttggaaac ctgaacttta
    25921 gttttatcat gcctgatgat ggggattcat tccgtgactt tgcgaaagta acacagccaa
    25981 tcatccaagc acttcatgaa ttgggtgttg ctggggcgga attgaaaggc cgaaatgatc
    26041 tagtcattga tggcatgaaa ttttctggca acgcgatgta tgctaccaac ggtcggatgt
    26101 ttgctcatgg aacaattatg ttcgactctg atatcaatga agtagtcaat gcattaaaag
    26161 taaaaaaaga caaaatcgaa tcaaaaggaa tcaaatcgat ccgttctcgt gtaacgaata
    26221 tcaaaccatt tcttccagaa gaaaagcaaa aaatgacaac agaagaattt cgtcaagaaa
    26281 tcttgttgaa aattttcggc gtagagtcag tagaagaagt aaaaacgtac gaattgactg
    26341 atgaagattg gaaaaaaatc aatcgaatct ctgaggaata ttatcgaaat tgggattgga
    26401 attacgggaa gtctccagat ttcaatttct ctcgccaaaa acgtttttct atcggttcga
    26461 ttgaagtcca tttgaatgtc tcagaaggaa tgatcaaaga agcaaaaatc tttggcgatt
    26521 tcttcggact aggagatatc tctgacgtgg aaaaaaaatt ggttggacag aaatatgata
    26581 aagcatctct atcaaaaata gtcagccaaa ttgatgtgaa gaaatatttt ggtgcgatag
    26641 aaccagaaga attgcttacc ttactttatt gaaaataaaa aatagcggaa tccacatata
    26701 tggattccgc tattttttat acaggattcg ggtaatttcg agagagttga acagtgatcc
    26761 gatcaatgaa agtatctttt tcctgcgtta gtgttcctgt gtaacgatcc gctacgtctt
    26821 tcatcatcat taagtcaaaa cgttcctgat catctaattc ttctgtgatg cctcgactaa
    26881 ataggtttgt atcgtaatgt gcactatgtt cgcaacaaat gacgaaatgt tcctcttgtt
    26941 cgtgtatcga caaataaatt tttcgataca gtggattttc gatggcatga gtttctctaa
    27001 aggaatgctc taaagcgtgg atcaaaagtt gagccagatc gtcatcgttg ataggaagtt
    27061 cttcgggaag atctgcttgg aaggttatct gaatattccg tttggcagct aacttctggt
    27121 aacgcgctaa gataagattt gtcacctggt ggttgcaaaa agaacacagc atttcgaaat
    27181 tttttgcttc ttctaatatc ttttccacat agtttttagc ttttgtttgg tccgtatgca
    27241 ttaaatcttg cagattctcc atctcttgga caaattccag gcgaagtgta tttacttctt
    27301 ggatatgctg tcgaagctgc tcataatgtt gctccaacaa atcatttttg atttttaaga
    27361 aatctacttg tctccgatct ttgtcggtat ttaagatgtg atcgattaga ttgtagctga
    27421 taataacgat tagaatgata atatataaaa tcgtagacca attgatcgtt gtttgataga
    27481 tacctaatgc cgtttggatg taaatcaagc aatggataat ggctaccaag acgatccaag
    27541 gtgtacaagc tacataaaaa cggttattct tatatgcttc gccaatgcat gcaagtgaag
    27601 tgactaaagt gcttataaca ctagcaatat tagcaatcgg cattaattca ggaagttctg
    27661 caggagtaaa agttgcataa agcagaacgc cgataaaaat caaaagttgg aacgtacaaa
    27721 aaattccgta ccatttttta tagtaaaaca ttcggctcca atagtagcag atgatgaaag
    27781 ctgacgttaa gataataaat aaatgagata aaaaagaatg aacgaatggt tcaaaaagaa
    27841 taccacttaa aatatcttct gatacagctt caaatcctaa tgccaaagca aagcaactta
    27901 agagaataag tgagtattca agacgttttt gtttatacat caagtagatc gtatagaaaa
    27961 aggtccctat agcgataaaa acagcgatta tcatcgtcaa tacttgcggc actgattgtg
    28021 aataaataaa actaatcaat ggtccagttg ttcctagata aactttcggt ggcagtccag
    28081 catagttctg ataaggagat gagactgtaa gggtcaattt ttttccgata taatttgctg
    28141 gtaaatctat cacagccaat gagagtccgg gattgctttc ttcctgataa tctttccgtt
    28201 ggtataaaat cttttcgcct agatatacag taatccattg atggtttcct ttgattagta
    28261 aatttgcttc gtccaacggt ttttcaagtg tcgtttccat aatgaatgtt tcgtctttat
    28321 ccacggtagg aatcgtacga gtgtacttgc tgtcaaactt aaaatcagga tcatcttttg
    28381 tatagaaaac ccacgaatct tttatttctt tcatttgatt gctgtccgca ggagaaaaaa
    28441 taaaataaaa gaaataagca attcctgtta aaaataacaa tgataatata atgattatgc
    28501 tccactttat acgtaaataa aaagattctt ttttattata tatgttatta aaaaaagaca
    28561 taaaggtatt cctttctaaa acttgtaatg ttagggtaaa cgttttttaa actaacgtca
    28621 attatttata ataaaaaatg agataaagat gaggaaatag aattagaaac atataattat
    28681 taaagggaaa catttttaca gctataattt gaaaaaaatt tatcgttatt atatgattaa
    28741 gaaggattga ataaagaaag aagttgaaaa cgatacctga ttacaaaaaa ataactttta
    28801 taagttctat aacgttgatt atatttttaa ttcttattct tctcgtcgtc ttggttcccc
    28861 gaatttttgg tttgactcct tatattattg aggacaaaga aatgagtcca tattatgcac
    28921 aaggaaatct gatatatgta cgaccgaaaa atacaaatga gattttagtc ggagatatta
    28981 ttacatatta tgaaaattca ggaaaaaaaa tctcgacgcg tcgcgtaata gccgttgaaa
    29041 acaaccacga agatttttat acaaaagctg atacaaagac ttatattgag cctggagtag
    29101 taaaaaagag aaatattatc ggtagtccca tctttcagat tccatatatt ggactagtcc
    29161 tttcaaaaac ggcatttgca tggattcagg ggttattctt tacgattgct ttttgcctaa
    29221 caggaatcac agcatggaat acctttgtag agttgaaaaa gaagcggaga cgaacacaga
    29281 aatttttgta agaatgttaa aaacataaat gtattccaga gatccaacga ctcttctgaa
    29341 catgaaacac gagaaaacac tcatgcagct tgtagattta gtcagttgtt cgtataatgt
    29401 gtgaagagga ggagtagtat gcgcaatgaa atgatgcatc gtcctgtagt caaagaagaa
    29461 ctattggatt ttatgcgtac ccagcaaaaa aaattaccgg gtgagctagg caaactggaa
    29521 gaagaagctc atgtagcaga agtaccagtt attccccatg aaacagttgt atttttgaaa
    29581 tttttacttg gacagcttca gcctgaacga atcttggaga tcggagcagc tatcggtttt
    29641 tcatcaagtg tcatggccac gacaatcgga gaaaacgctc atgttacgac aattgatcgt
    29701 ttcgatgtca tgatcgagaa agctaaaaaa aactatgaac gtctaggact gacagataaa
    29761 gtgactttgc ttgaaggtca agcagcagat atcttgccgg agttgtctgg tccgtatgac
    29821 tttattttta tggatagtgc aaaatcaaaa tatattgaat ttttacctga atgcttacgt
    29881 ttgttaagaa aaggtggcgt tttgatggta gatgacatct tccaaggagg aacgatcttg
    29941 ctcccagatg aagagattcc ccgtggcaaa cgagctatcc atcgtaaatt aaatgaattt
    30001 cttcgtgttg tgatggatca tcccgatttg acatcgacaa tcttgccatt aggtgatggt
    30061 gtgatcttaa tgacaaagga atcagaaaca atcgagttgt gatacatgat tatggcagcc
    30121 tgtagataat cgattgaatc gagggctcaa ttcgaacttg atgtaaaagt ttctatattt
    30181 gtaagtcaat taaaggatac aggaaatcta aataaacagg agtagcatga agtcaatgta
    30241 gctcctgttt tcaattgata accttagaac agctgaagga gaatgtctcg ttttaaaaga
    30301 ttgatttagt agttgggcca aaataattca ttatttgcta attaaataac tatttcgtat
    30361 tgacagaagc aatttaatta tctataatta ttgatgttcc gctgattctt gtcatagtag
    30421 ctcagctgga tagagcatcc gccttctaag cggacggtcg ggggttcgaa tccctcctgt
    30481 gacgctaaaa aaccgttaga aactgaggtt tctaacggtt tttctttgtt ttttgtgcca
    30541 atattgtttg ttttcaaagg caaataaaat aaaaaaacat ccttatcgag tacaattata
    30601 aggagcaaaa aaataaaact catcagaaaa gaggaagaag atatgacgat actatctaga
    30661 attttagtga ctctagttgc aattgaattt ttttatatca tgtatatcga aacggtgaga
    30721 actgattctg acacgacaag tagggttttt aaaatgagta aagaagagtt aagcagaaaa
    30781 tctgttcaaa cattgtttaa gaaccaagga gtgtacaatg gactattagg agtgggacta
    30841 ctttatggag cttatctttc ttccgcatct aaagaaatca caagtatgct attaatatct
    30901 attttttttg ttgcattata tggcagtttg actagcgatc gttggatttt actcaaacaa
    30961 ggtgggctag ctctgttggc attgctttct attttcttat aaatatagaa aaaataagaa
    31021 tttatctaac aggaaacttt gtgaaaaata ttactttatc tcatgaaata tgatattcta
    31081 atgaatggta ttttagaaaa tttcatgaaa aaggagttag ataggtgttc aaaaattatg
    31141 tttcctcact aaaaaaagaa ttttcaggtt atgatctttc aagatttcag aaagatcttt
    31201 tggctgggat cacagtagct gctgtagcat tgcctttagc cttggctttt ggggtatcaa
    31261 gcggagctga tgcggccgca ggtttgatta cggctatcat tgccggtatc gttattggcg
    31321 gtttatcagg cggtttttat caaatatcag gtccgacagg tgcgatggca gctatcttga
    31381 tgtctattgc ggcaacaaag ggaatgcaag gtgtattgct tgctactttt ttagcgggtc
    31441 tatttttact tgcagcagga cttttccgat taggaacact gacttctttt atcccagcac
    31501 ccgtaattac ggggtttact tcaggtattg ctatcattat tgcattaggt cagatcgata
    31561 atttatttgg tgttcactcg gaaggtacaa acgttattga aaaactaagt agttatcaaa
    31621 cgataggatt tccgataaac acagcctctt tgatcatggg agggctagtc attttaggga
    31681 tgtttatcta tccaaaaaaa tgggcgaaac gaatgcctag ttcgctgctg gctatcattt
    31741 tggcaacagc agttatgcta ttgttccatt taccagtagc aactgtgggt aagattccac
    31801 aaacattgat cagtagtaat cgtttgaata tgggagattt tagcttttct gccttgcaac
    31861 aagtggcagt tccagctatt agcattgctt tattaggaat gattgaaagc ttgctatgtg
    31921 gtgcttcggc tggccgaatg gcaaataagc ccttagatag caatcaagaa ttaatggcgc
    31981 aaggaattgg aaatctgttg cttcctttct ttggcgggat tcctgctact gcggccatcg
    32041 cccgtacaag cgtggccatc aaatctggtg cacaaactag attagctggt atgatacatg
    32101 cagttgtctt actgctctcc atgcttgttt ttgcgccagt gatgtcacag attccaatgc
    32161 cagcacttgc aggtgttttg attgtgacgg cttggcgtat gaatgaatgg gaaacgatca
    32221 aggagctatt tactaataaa tattggtcag cagttctatt gtttatcctt acgatgattt
    32281 gtacagttat ctttgattta agtattgcta tcgtgatcgg ggtgatcggc ggctgtgtat
    32341 tttttgttgt caaaagcgct gctatcacga tttcattaga aacgatcgat tggcaaagaa
    32401 tggggctgcc agaaacggaa aagctgaata actggacagt agtgtatatt agtggtccac
    32461 tgttttttat gtcttcagaa aaactcaaaa ataaattgga agaactaaat catcacgatg
    32521 gtgtcatttt ctccatgcga ggcgtaccaa gtattgattt gacggcacgt gcagtttttg
    32581 atgaatttca agaaaaagca gagcaaagag gacaaacgat tatctatact tccttgcagc
    32641 cagaagtaga aaaacaactt tctcacttgt gggaacatac attcacgcat caacatcaaa
    32701 cagttgctca tgcgttgaca gctttacatg cgaaatatac tgcaattagc cattaaaaca
    32761 atgaaaaaga acagacaaga ctgtaaactg aagattttat ggtcttgtct gttcttttta
    32821 atattgctgt aaaagtgaag ctagtacttg ccagtcttga tggataacag gtacaaggga
    32881 gcgattgtag aaaatagctg ctggatgaaa aagtggaaga attcgatagt ttttttctga
    32941 ccattcgtag gcatcctttt gttctgttaa ttgtaagaca ggccctgtat agagttgacc
    33001 atgatttttg gtaaccgtat attctttgcc aagtaaacgt tgaagaccaa tgtttccaag
    33061 tgtcgcaatt aattttggct ggaccttcat cagttcgtaa tccaatattg gtgcatgcgc
    33121 aaatacttcg gaacgagtag gtgtccgatt tggataaaca gtttctgact gttgtgtccg
    33181 tttattgatc cgatgagtca cgcgatacgg gcggctcctt acagcactgg tgatatacac
    33241 ctcttctcgt gttaagccag ttgaagaaag tgcctgcatc aattcttttc cagcttgacc
    33301 gctaaaagga acatgcgtat caatctctgt tttccctggg gcttctccca cgagcatcag
    33361 tttaggatga agaggtcctt gcccagcgat aaatccttct acaggacggc cgttcattct
    33421 taaagaagct tcttgtatca gttcttttgg gtattccatt tatgctcctc ctttttcttt
    33481 tattataaga ataaaagaaa cacaatgcga ctattttatt tggtagaact gtgaggatag
    33541 aaaagcagaa gtattgcttg aatttttgtt ttactttttg attttaaatt tagttgacta
    33601 acggttgctg ttctggtaag ctagtactgt tgtaattgtg gactccacag ctacaaccgc
    33661 tcagaacaag taataagcac agcatatgct gtcacttcgg atggcgagtc taagtatatc
    33721 agaaggaggt gctttatagc atgtacgcaa ttattaaaac tggtggtaaa caagtaaaag
    33781 ttgaagtggg acaagcaatc tatgttgaaa aacttgacgt tgaagcagga gaaaaagttg
    33841 tttttgacga agttatctta gtaggcggag aatctacaaa agtaggcgct ccaactgttg
    33901 caggtgcaac tgttgaaggt actgttgaaa aacacggcaa acaaaagaaa gtcgttactt
    33961 tcaaatacaa acctaaaaaa cattcacacc gtaaacaagg tcatcgtcaa ccatatacaa
    34021 aagtggttat cgacgcgatc aacgcttaat agcgtttgag aaagaaggcc tatatatgat
    34081 caaaggaaca tttaagcgaa atgacgcagg tcagatcgtt tcattcactt tgaccgggca
    34141 tgctgatgct ggtccttatg gaagtgatat cgtttgtgcg ggtgtttctg cgttagcgat
    34201 cagcactgtc aacgggattg cctcattagc cggttttgag ccgattgttg aaatgaatga
    34261 agaagaaggc ggttatcttt ataccgaagt cacttcagga atgacacaag aacaaaacaa
    34321 tattgctgag attctattag aaaatctttt attaggatta caatcgatag aagctgaaaa
    34381 ctcagaatat atccaaatta aaacaattaa tgataaatag gaggtgcaga ccatgttgtt
    34441 atctatgaat ctacaattat ttgcccacaa aaaaggtggc ggttctacat ctaacggccg
    34501 tgactcagaa tcaaaacgtc taggtgctaa acgtgctgat ggacaaacag ttacaggtgg
    34561 atcaattctt taccgtcaac gcggtacaaa aatctatcca ggtgtaaacg tgggtatcgg
    34621 tggagacgac actttatttg caaaagttga tggcgtagta cgtttcgaac gtaaaggccg
    34681 taacaaaaaa caagtgtctg tttatccagt agctcaagaa gcttaataaa taaactgctc
    34741 tatctcttgt gtagcaaggg atggagcttt tttgtaaatt atgggatttt ttttgaccat
    34801 acttttgacc atactttctt agaagttaat ataatttgtt agtttttgtg ttgtttcttc
    34861 ttttttgctg ttagtaatgt gtgtatatgt gtccatagta gtttttatat ttgcgtggcc
    34921 cattctttct tggacttctt tgtgatctgc gcctgcctca tatagcattg atgcatgtgt
    34981 atgtctaaaa ccatgcagac cgatataagg taagccggca cgctcacgat atatattgta
    35041 tctttctgcc actgactgat taattgtgaa attattttcg tcattggtaa acaaataatt
    35101 agctttagta aatcctaatt gaaataagta ctttctttgt tcgagcttcc aaaattttag
    35161 ttgctttata gttttttcat ctaaataaat tattcgattt gattttttgg ttttaggagt
    35221 ttcagatata taatatttat ctgaacgagc tacagttttt ttaatggcca tttcatttgt
    35281 tttaaaatta atattgtccc aagtgagtgc taatatttcg ccgattctac atccgctgaa
    35341 tgctaatagt ctaaagagcg tatagtctct gtttttaaaa tatggattct gttcttgaga
    35401 tacttcattt agaaataatt ccaactgttc ttttgtatat aattttaatt tcttttctgt
    35461 tttaatttgt ggctttggaa taagtaaatt taatgttgga ttatcagatg ttaaaccaat
    35521 atttatagca tacttaaaaa ctttattcat atagtttatg aaaagagggt actgctttga
    35581 agttcctttt tcactccaat catttactac cttttgacaa tatgccgtat taattttaga
    35641 aagcttaata tttccaaatt tgggatatat atgtttatta aagattattt tagtccttga
    35701 atatgaactt tcttttacag tttttttgta gctttctagc cataattctg ctgcttcttt
    35761 gaaagtagta tttgtacttg tgggcattcc tgttttttgt aattctagtt ctaacctaga
    35821 aagtgctgtt tttgcttctt tttgtgttct aaatcctcgt ttagtagtat attttttctt
    35881 tccagttagc ggatcaatac cgagataagt tttaaagtac catgcttttt cgccattttt
    35941 ctttttatat tgttttatca ttgccatcaa tattacccct attcaaattc taattctgcc
    36001 atgctatcat tgaaaaattc gatcagtcct tgttcctcta taaattcaag ccatatttcc
    36061 ttcccgttaa ttaatggaac tcctttttct ttacgaattg tttgatacaa gttagtcacg
    36121 acttgtttcc acatttctaa tacagcttta tctattttct tatcatccca gcgttttttt
    36181 tggggtctat ttttgttttt ttcaatttga taattagtag ctttgatgat aaatgtttcg
    36241 tatccggttg tgcttttttc taaccattct ttgaattctg tatatatcat ttttgttcac
    36301 ctttctattt ttgaacgtat gttctttttt gcttaaaaaa ttttagagag ctgcataaca
    36361 atagatgcga gcttttaata attctttcac aagtggttcg aagcgaacgc ttaaattgtt
    36421 atcttctata aatttcatgt aattgatatt gtgtggttct aattcagact ttgcaatata
    36481 tctatctaat aatttttcaa tcatgaagcg atcagcctca tattccattt ttgaatggag
    36541 agagaatgct aagttatata aaaaataatt atcatgatgt ttagaagcgt gtcctaattc
    36601 atgtaataag gttctttgtc ttctatactt agataaatta gcttttatta ctatggtatt
    36661 catacaagca acataatggc catcggcatc aaggttttca cgctcttcta ccttaacgcc
    36721 gagttcatta attatcattt caatttgact gtccaaatga ctcacctact tatttagtta
    36781 tcttttttct ctaaatatgc ttcaatcata gctgatagta tttccctatc attatcggtg
    36841 agcggttttc cgtcgctact cataacagat gctaaagctt cttccacggt tagttttctt
    36901 tcctctggtg caactcccgc gttaggatca tctgtgcgtc ctagcaaata atctgttgat
    36961 acatggaagt agtcagcaac tttttgcaaa gtttctattt taggactttg tgtcttccat
    37021 ttataaatag agttttttcc aaatcctatt ctattttcta attcagaaat agaaatacct
    37081 ctttttttac agagctcttt aaccctttcg aaaagcatgt caaatcaacc ttcctagagc
    37141 gtacgataat aatttataca aaaagataga aaggtagttg acaaatctat ccaattgtct
    37201 tatatttaac tcgtaagcta atttaataag ctaacaagca acaagaaata cacctaataa
    37261 aataaacgaa ttcgaggtcg gcaaactaag aatgttaatt tttagggttt ttctaggctt
    37321 atttaactgt atataaatat aagacagttg gatagaaatg tcaataaaaa ttagctaatt
    37381 ttattagctt acatatttaa agaaaaggag gaaacatcat gaaacaaaat aaaaaaactc
    37441 cttccgcaaa aggagcttta ataactttaa attttaaaga ccaaaagttt agtgctgttg
    37501 ttgatgaaat tcatccacaa gagcatcaaa aaaattcttt tgcaactgac gagaaaaaga
    37561 ctcagaaacg ctctgagaaa gttcttcgaa taattcaatt tctgttggaa aagattcgtc
    37621 attttggaag tctattttga attctttatt aacagtcttg aatacggttt gtttgaataa
    37681 ggcgtcgaca tccatattaa attcttccat actattttca cctcgctttc tattcaaaat
    37741 tatatcaaag aaaggaatga gcaaaaatga acacaccgca aatttttaac tttgaacaac
    37801 atgaagtaag aacagtaaca atacatgatg aaccattttt tgttggaaaa gatgtggcta
    37861 aagttctagg gtaccaaaat ggtagtagag atattaacag acacgtcgat gtagaagatc
    37921 ggcaaaacta ccaaaacggt acttttgaat cgcctagagg gttaactatt attaacgaat
    37981 caggtttgta ctccttaatt ttaggaagca aacaaccaaa tgctaagaaa tttaaacgtt
    38041 gggtgactag cgaagtgttg ccagcaatta gaaaacatgg aggttatcta actccagaaa
    38101 aagtagaaga agctttgctt aatccagata caatcatcca attagcaact aagttgaaag
    38161 aagaacgtac tggaagacta atcgcagaac aaaaaattgc agaatacgaa cccaaaattt
    38221 cgtatttgga tagtatctta tcttctacag attcagtaac tattagtcaa atcgcggctg
    38281 attatgggat gtctccacaa cagatgaaca aactacttca taagctaggt attcagaaaa
    38341 aagtaggcaa ccaatggtta ttatgcaaaa aacacatgag acagggttac acaaaatctc
    38401 atacaactga gattccgaaa tctgatggcg gaacaaaagt tgtgatgaat acaaaatgga
    38461 ctcaaaaagg gcgtctgttc atttatgagt cgctgaaaaa agaaggatat attccagaaa
    38521 tcgatttatt agaggaggaa tgacgatgtc acaagaattt attttgaaag tcagaattca
    38581 attagcaaaa tacggaaagt ctcagaactg gttagctgat actatcggaa tttctagacc
    38641 atatatgtcg gacattatga atgggcgtag aaaaccagat aaacaaatca agccaattga
    38701 ggcagcacta gcagagttag agaaggagaa ataacatgca aataacaatt ccagataatt
    38761 tagtagtttc cgaattaact acacagatta cgaatgctgt tcttaattca ttggacgaac
    38821 gattacacct tatgaacaaa tcagtagagc ttcctccata tccaaacaaa tcagaggtaa
    38881 aaaaagtttt aggcattggt gatgacaaat taacacattg gataagccta ggcttaaaaa
    38941 cacagcagtg gagcaagtta gacatcagaa ttgaacgatc ggaactccaa agatttttga
    39001 aagaaaactt tgagttctaa aggcaaagga gaatgatttt atgtcctaca cattgcaaca
    39061 agaacatcaa attctaggtc tgattaaaca gcgtagaaaa caattacaag atgatcgtgc
    39121 agcgcttaga aaatccgatg agctatcaga tagacaagct gaactaattg cttctgaact
    39181 tgaggatttg agaatgctag aaataaaaaa tagggagatt agattatgaa gaagacagac
    39241 acacttttta taggattcat tttggggtta ttagtgattg tagcgcacca aagtattatc
    39301 ggaggaagct tgttcgcagc attgatggtt ttaatcaatc tgcttgactc aaaagaaagg
    39361 agcaactatg gcacgagaag aagcgctaaa aatcggtaaa gtgattgctg ataattggtg
    39421 ggcaaatagc cgttctatta ttttaagcaa gcaacatatc gaaaagaaaa aagcatggca
    39481 acaaataaaa aagtgactcc gccgaccaaa gcaatgagtc actcaaaaaa tcataactaa
    39541 ggagatttta acatatggaa aatgaacttt ccactctaga tcaatatttg gccgatcctg
    39601 attggggcaa atcgaattta caactggttg ctaacaagta cgaaaaatca attgatgaag
    39661 caattgttgc attgactgat gcagcttcca taaccgctga tgacagtaaa tggaccaaga
    39721 gagacttggg cattctaaaa cgaggcgtta attggcttga agatcagtac agagaagaaa
    39781 caaaagagaa gtgatatgag tgtttaaacc actaatagat tcatactcgg cggtactaaa
    39841 gaaatttaaa ggtaacgaca ttggtgcaac aatcaacgaa gaagtaaata tcgaacggct
    39901 gaaaacgatg tatgacggat atgatggcga tcgagtcatt gaaattcgct ttattgatcc
    39961 acgacggttc acagtacagc aaagaaactt catctatgcg ctcataggcg atattttcat
    40021 cgatacaggc atgccaacgg acttctggaa ggaattcttc tacttccgtt ttgaaggtgt
    40081 cacagggcgc gaaataagcc tcaaagacga atcgaataca accgtgagtg atgccaatat
    40141 cttagcgaat atcatcctag atttcatctt tgaacatcat attcctttca aagaaggata
    40201 tgagatttta ccagcgaatc aagagtatta cttctacaaa tgcattacaa aaagagtctg
    40261 ctgcatctgt ggcaaaacag gagctgatat cgatcacttt gacaaagcgc tgggaagacg
    40321 aaagcgcaaa gaagttgatc attcagagta cacatttgca gcactctgca gaatccatca
    40381 cacagagaaa cacaaaatag gtgtgatcaa tttcaaaaat aaatatcaaa tcaaagggat
    40441 caagttaaac caggaaacaa ttaaaaagtt aaggatagga ggataaattt ggctgagata
    40501 agttggatca aacttaaaac tactatgttt gacgatgaaa aaatacgatt aatccaagct
    40561 gttcctgagt cggatgccat catcgttata tggattcgat tactagtttt agcaggaaag
    40621 actaacgacg atggtctgat atatatccag aggaacatgc cttataccga agaaatgctt
    40681 gctacattgt ttggcaaaaa cgtaaatacg gttcgcttag cgctaactac attggcaaat
    40741 ttcaacatga ttgatctaag cagtgatgga ctaattgcca tcagtaattg ggaaaaacat
    40801 caaaatatcg agggtatgga taaagtaagg ctaaaaaatg ctgaaagaaa ccgtaaatac
    40861 agggaaagaa agagacagga acgtctcaaa ttggaaaatg acgttagcgt gacgtcacgt
    40921 gacggtacag ataaagatat agaagaagat aaagatatag ataaagaaga aaagaaaggt
    40981 aagtattctg acgaacactt acgccttgct aaaaagttgc aaagtaattt aactgaagat
    41041 tttccaaaag aaatgaacaa agtagatatc gaaaaatggg cagacacaat caggttgatg
    41101 gaagaaagag ataaagcatc tatagaagcg attgagtatg tgatcaattg gctacctaca
    41161 aatgaatttt ggtttggaaa tattagaagt gctaagaaat tgagagaaaa atttgagaag
    41221 ctcaaattcg aaatcaaagc agacaagaag aatcataaaa agcaaagtca aaaactacag
    41281 tacagcaatc ctagtgaata tgacgacttg ccaatttaaa aaggagatgc atcacatgga
    41341 aagcctagca aatgctatgg agaaactaat aagaagagta ttagtgcaaa gcggaaaatg
    41401 tccagaatgt agcgaacctt tgtatagttg gcgagctaaa aataaggatg gttcagaacg
    41461 ttgtaaacca acatgcatga gttgtggtta taaagcgtta cgtgtgaaag aggatataca
    41521 gaccgaacgg atatataacg atagcttaaa agcacgagcg ttgagttttt ttcaaaatgg
    41581 ttcggtatta acagataaaa ctttgtttaa atgcaaaatg gaaaattatc acgtagtgga
    41641 tcaagaaaca aaaatcgctt tagaaaaagc aaaaagctat acgaatgagg ttctgctgaa
    41701 ccatcctgca catttcattc tatcagggaa atcaggaagc ggaaaaagcc acttatcaat
    41761 ggccacagct tgggaaatac ttgagcgctc aaattatgac aagaaaatac tttttataag
    41821 ctatcaagaa ttattagagc aaataaagtt ttcttataac aatactgaac tgagaaaaga
    41881 aattgaagga tcgcttatag ccgatattaa aacaactgat ttggtggttt ttgacgatat
    41941 tggagctgaa ttaggtagtg gggtatcaaa tagtaggcag tttacaaaca acacgttaaa
    42001 cacgctctta gaagccagac agaacaaggc aacgatcatc acaacaaact tatctggtcc
    42061 tgaactaaga gaagcctacg gtgaaagaat tgtttctagg atatttaaga attcagaagg
    42121 ttatgcgctg aaattccaac aaacagcaga caagcgcata aaaccagtga aaggtagtat
    42181 cgcatgaata aataccgtaa taaaaaaact gttcatcgag gtatcaagtt tgattctatc
    42241 gcagaagcag agtattacga tctagccttg tggcaagctg aagcaaatgg ctggaaagta
    42301 aaacttcagg aaagatttga gctgatgccg aaatttgaac tagacggaaa gaagtatcgc
    42361 aagatcgagt atattcccga cttcacattt tataaaaacg gcaaacttgt caaagtcgta
    42421 gatgtcaaag gaatgcagac aaaagacttt aagatcaagg caaagttgtt ctgtcatcaa
    42481 tatcaagcgc cgttgatatt agctaaaaaa tatcggaata cgttcaagga agagcgtttt
    42541 taacgaggtg gtccatcatg acaacagaag aagtgattca aatgcgtatt cgaaacattc
    42601 agcgtgaaat tgacgatcta gaacgaacaa aggcagtgat ggtcaatgaa acggcgagaa
    42661 aggcaatcga tttgcacata gagaacttaa gaagggaaat tcgtagattg gaggaatgag
    42721 cgtggataag aaagcgacaa tgaaacgaat tgctgaatta accaagtcag aatcttggca
    42781 ggaagacaaa gaaatagttg cagaagtcca aaagctcggt aaatcaatgt ggactgaaaa
    42841 gcctaaacgg aaaacgccga gaaaaattgc aatctggcat ggtgatcgaa ttctagtaac
    42901 aggtacagct gaacagttat ctgaaattac tggattaagc aaaaacatta tctgggatag
    42961 agctaggagc ttatggattg attcaaaagg acgacagttt aggtatgtgg aggagagata
    43021 atggatctca ttacacaata cagtgacatc atcctcaaga aaatcatgat gaagattcag
    43081 aaagacaaaa aatcaaaaga acgagctgaa ttagttaagt tagaaatggc tgaaacagga
    43141 gcaggagtgc gaagtagcag gcattggaaa gcagcagcaa acattgaatt ttattacaac
    43201 gaaattcaaa aagggttcga tcagatgcgt gagctggatc ggcaaacaaa ttggagcaag
    43261 aaacttcatc aagatcgttt caaatttgta gagaagtata aagaaatatt agaagagtat
    43321 ttgaggagga cagcaaatga taaaaaaact cgttcaattc agcatggatt tatatgatat
    43381 cgaatcagga gcaacactat ctgtggaatc ggaccatcta atcataaatt ttggtggaaa
    43441 gcgccagatt attttgtggg tagttgatga tgtactgttt ccagaaattg ttcatgattt
    43501 cgaagaatca aaagcggttg agtttgaaat agtgaaaaaa gtaatggaat tgattgaaaa
    43561 atacgaggag gacagcgaat gataccgaag tttagagcgt gggatagaga aaaagacaga
    43621 atgaaggcag tagctttggt tgaatttgct gaggcgtata aatcaggaga actcatttca
    43681 cgtacaaaat aaaaaaagtc ggattcctcc gactatgaat aatatttccg acataagtat
    43741 tatatcataa ttgggggaat cagaggatgg tacttttcga tgtaaagaaa tatgagacac
    43801 caagcgcgaa agatgttgat atggaacgca caaaacataa tgtcgctgtg tttctttcag
    43861 catatctatc agctagatgt agagtagggc aacctcgaga accaaaagtg acagcatctt
    43921 attccttggt tccaccttct acagctgatc atatatttga agcagaaaga atgctgatcg
    43981 ataaagaaga agcacaggaa gaatttgagt atctgcataa attgtttgtt cgaggctatt
    44041 cagcgataca gcatccgcat aagcctgatg tgactgaaag gcgcaagaag atattctatg
    44101 atcgttatat caatggtctg cccatttatg taactgctca aaggaataat actagcgaag
    44161 aatcggttaa agtagaatca aacagaatta tcatccaatt tgcttcatcg ttagaactgg
    44221 ttgctttcaa gtagccagtt tttacacttt ttataccctt ttattaccaa tttggtttcc
    44281 attttatacc ttttttatac caatcactta cctattcaac gttgtattat gatagtgtcg
    44341 aaagattagg aaacaggatc gacaaaataa aatgtaaggg aggaaatctc cctcatcgtt
    44401 taattaagct tcgataggca gcagcggaaa tattaagaat aaggatgtga atttcaactc
    44461 cttctaaatt gttcttatta tctatcatcc gttgctgtct attaatttat gtattggagg
    44521 gtatataaaa tgaagatcgc ttccgaaaaa attgatcaac taacaatttt agtaaaccaa
    44581 agaatcgaag atattatcaa agaattcgaa ggtgaaaaga ttaagaacat aaagcttact
    44641 ttaatggatc ataagttcga attaaattcg acaaaaagga agatcgttga gcttatagaa
    44701 gaagaaatag ctctccagat caatgagcaa caattagcta attaatttat gtattggagg
    44761 ggaaatgaat ggaagtcgaa caaataaagt atcaaggtat ggatcgggaa cggttgattg
    44821 atattattga gcaacaacgt ttagaattga tccaaaaaga agctgtatgt aaagagtaca
    44881 aaaaacattt ggaacaagta attgagtatc attcagtaga aaagtacaga tcagtggtgc
    44941 aaaaaaatag agcaacgagt cctgattctc gtcaagatgt atcgattagt atcaagacgc
    45001 cagtatttaa ttctgttaaa tcagaaaagc tagaaagtat ctcaactgct ttggtagaaa
    45061 aagcagctga aatgaactcc ctagcaaatg aagttagtcg tcttatttag tcatatcgat
    45121 aacttgagag tctttggtga ttccgttttt tatagaatta atggtttgtt cagcggaata
    45181 tttagtcaag tatgtttcgc tagttgctac tacttcattg ttattggact taataacgaa
    45241 gtaatattgg ccattagttg cttctcttat aacaaagtac aaatttttca ccacctataa
    45301 tttatttcag cggaccactc gctgataatt aaaattataa gctatgtata aaatttcaca
    45361 atatcaattt gtcgctgtgg cggaaagggt agacgcaata aaggatgaat gggaaaagtc
    45421 aatgagtaat tatctgaaat ttacaagtcc cctgtttagt tatctaagat tatttgataa
    45481 tattatttct gaggtgatag aaaatggtgg gcgtagttaa atttcttggg tttacatttg
    45541 atggatatgg tacgttaggc gaatgggtag gtattatttt gatagtgatt acaattcaac
    45601 aaacatcaaa gtactactgg tattctaata gttctaaaat tgtatcaaga ataactgaag
    45661 gaataaccaa aaaagatatt actgtcgtcg gtaaatatgg gtttactata actttattaa
    45721 atgaagggaa atctgaaacc gcaataaagt tttgcgggat tataggtaaa tataacattt
    45781 tccaacgttt tatcagaaag cattggtaca aatatattta caaataccct gtagttaaaa
    45841 agcttattga aaaatatgtg gaccacgaaa tatcttatct caatccaaca gaccttatta
    45901 atcaagaatt ccagaatatt ggatacttag aaaaaggtct aaaaatacca attgtaaatg
    45961 aaagtttagt tactgcaatg gtagctaaca taaagaacag caagaaatta gaaaagagat
    46021 ataaaaaatg taaaaaattg aaattttcct ttttattctt agaatataat ggcaaagaat
    46081 attatcaaga tatttatgtt ttgcgaggct cgaacttata taaagacatt aaaaataaga
    46141 tttcagtatg agatttttta aaattcgtag atcactcttt gagtggtctt tttattttgc
    46201 gtaaaggagg cacgacgatg tatagaccac aatacttaga acagaagcat gaagtaatca
    46261 ctgtgcaaaa cggtaacggt gagatagtac gaaagtatag aagaccaata aagagcgata
    46321 catataaacg gaaggaaagc aatgaagtta ttccattgta tggcaaaaga atagctaagt
    46381 attaaataag attgcgaaag gagatgggac atgaccgagg aattctatag atggttatta
    46441 cagttgataa gagaagatcg tttggttaag ttctatcagt ctcctaaatg gcgcaggctt
    46501 agagagaaag cgatgaaacg agatcactat gaatgccaag agtgtagaag actaggtaag
    46561 tatcatagag tagagaacgt tcatcatata aaggaagtca aggatagacc tgacttagct
    46621 ttagatttag ataatcttat ttgtttatgt gttgaacatc ataatgaagt tcatggcaga
    46681 tatcttacag cgttagataa acaagagaag aagatagaaa gcttcgctaa cttcgatgca
    46741 agtgaaaggt ggtaagtgca tgatcatcaa tgacaatggc agagagtatg atacagaaaa
    46801 gattgaagag tattcatctt atactcaggg attaattaaa cgtttgatat acgttcgcta
    46861 tgtaggtatt agggatctgt tatcagataa ctgttgtagt aaatacaaag tgaatcaagt
    46921 aagagaagcg ttgaataaag ataataacgt cgaaagaata aaaaatgttt ttggatatag
    46981 tattgaagag attaattatt acattgactt cgctgaagct ttcattccga tggtgagata
    47041 accccccctt aaaataaatc gcaaattttt tgggggtgat gaaacggagg gggctgtcag
    47101 gaaaagagat tttttcgaac tttatcatga aaggagggct aaaatgttta aaaacgaatt
    47161 gtctcaaaat cgctacagag aaaaattacg ccgctcttta ataagccaat tggaaagtca
    47221 gaaaacaaat attgagccat tcttagataa tgttgatcgt tatatcagtt tatgggaaac
    47281 ggcgatatca ctggaagaag atatatccga gaacggcatt agactggaga atggtaaaaa
    47341 gaatgaatca gtagcgttgc ttgtttctgt caacaaacaa atgggattga tgttggataa
    47401 acttgccatt actcctgaat tggtaggtga agcaaatgaa tcaattcctg agttataagc
    47461 atattgaaaa ttggttcaaa gctatagaag aaggcactat caaggtatgc aaagagcaat
    47521 tattgctaaa aaattatcta gaagaaagag tctttactag agaagatatt tacttcgata
    47581 agcagatggt agaggattca atcaatatac cagcacaata ctttccattc gaattaattc
    47641 cgtgggaaaa atttctacaa tgttttattt atggtgttcg atggaaaaaa gataaaacac
    47701 tagtgttcaa tagatatctt tcattaatgg gacgtggtaa tggtaaaact ggttttgctt
    47761 cttggaacaa cttctttcta ctaaccgcta aacacggtat taaaaattat gatattgata
    47821 tctatgccaa taatgaaagc caagcaaaga ctagttttga tgatgtattt aaagtaatta
    47881 aagatcatcc tgatttagat aaaaaagtat ttaaagctac gaaggaagtt attcaaaata
    47941 tcgctacaaa cagcaaactt cgttataaca cggcaaatgc tagaacaaaa gatgggaagc
    48001 gaccaggtgc aaaccgcttt gatgaaattc acgaaaatga agattattca atgataaatg
    48061 tggctacttc tggtggtggt aaaattcgag attatagaga attttatgat acaactaatg
    48121 gtcatgttcg tggtggtccg cttgatgaca ttatagaaga atcaaaaatg attctttctg
    48181 gagaacttgg aattgacaag gatggagcag aattttctag tttgtttcca tttatttgtc
    48241 gcttggataa cgataatgaa gttgatgatc ccgacatgtg ggaaaaagct tgtccaacta
    48301 ttaattacaa tgcagattta aaacggaaaa tgtttcaaga atactctcaa atgcaacgta
    48361 atgctggttt aagacttacg ttcatgacca aacgaatgaa cagacctatg gaagatacac
    48421 gatttgctgt tgcttcatat gatgatgttc tgcatacgaa agaaaaagaa tttcctgaaa
    48481 aaatggatga agtgatagga acagtcgatt ttgctgatag acgagatttt gccagcgttg
    48541 ggttgctagg aaaatacgat aaagatgttt attttacaca acatactttt atccacgaat
    48601 cagcccttcg attacaaaac atcaaacgag aggttataga tatttctata gatcaaggaa
    48661 aatcacagat cgttcatgga aaaaatatag aagctgatta tattgtaggt tggtttcttg
    48721 aaatgagtaa taaatattat attaaaaaaa tcgctatgga tatgtaccgt gcaaaaatat
    48781 tgaagcccgc tttagaagaa gcaggtttta ctgtggaaat tgttcgaagc ggatctgtta
    48841 cacatggtat gttaaaagat ctggttgatg acctttttat taatcaacgt ttattttttg
    48901 gtgacgatgc gattatgcgt tggtattgca tgaatgtata tgaagagcat atttctaatg
    48961 gaaatatacg ctatgaaaaa atagaacctg aaactagaaa aacggatggc tttttttcat
    49021 tccttcatgg tttgaatttt ttagatgata tttatgattc tgctcctgta acagtcacaa
    49081 atagctcagt agaaaataca ggaactggat ttactcctct agtattctaa cttgaaagga
    49141 ggtgagaaag tggggatttt tcaaaaggcg gtaggatact tcacaaaaaa agcaacggtt
    49201 cctttagaag aatacttttg taaattgcaa gttgattttg tgtatcgaaa atttgcgatt
    49261 gaaacttgta ttgatttgat tgcaaatgcg atgagtaaag cggaattcaa gtcatatgaa
    49321 gatggaaaaa ataaaaagaa tgatctttac tataggctga atgtagctcc taataagaaa
    49381 aataatgcaa cagaatttag aaaaaaactg atcaggagat taatattcta caatgaagta
    49441 ttgatcgttt ctccgtctaa taattctagc gaaatattta ttgcggatag ttgggatgtc
    49501 acagaatatg cattgaaaga tgatgtgttt tctcaagtgc aaattaacaa catagtcctt
    49561 gatagagaat ttctagaaag tgatgttatc tatataaaat acgcagatca acaaattagg
    49621 caactagtcg atgcgtatta tcaagcgtat gggaaactca tttctagtgc catgaatgtt
    49681 tacaagcgct ctaacgctcg tagatacgta ctgaaaggga atttattccg accgcaagac
    49741 aatacaacac aagatcaaat caataaaatg atgacatcac aatttaaggc ttttatggaa
    49801 gctgataatg caggtgcggt atttcaatta caaaatgagt acacattaga agatttcagc
    49861 ggaaacttcc aaagcaattc aagagatata aaaaacttaa tagacgacat ctttgagatg
    49921 acagcagcag cgtttcacgt tccgaaaaac ctactaaagg gagacatgag tgggttatcg
    49981 gatcaagtgg acgctttttt aatgttcgaa atcataccga ttgctgaact tattcaggat
    50041 gcgtttaacg ctagtctcta tgaagtagaa gaatacttgt cagggaattt tgtacgtgtg
    50101 gatacaacta tgatcaagat tactagcttc aaagatttgg ttgacgctat tgatgtaggc
    50161 attagaaatg gagtatttac aatcaacgaa ggaagagagc gcgttggaaa tgatcgctct
    50221 gataaggcga tggcagatga aatatttata actaaaaaca atcaacaagt atcgaaagga
    50281 ggtgaggcga atgacgacaa tgaaaacatt tctagcagta aagaatgaag gcgcagtacc
    50341 gcaaattttt attcagggat ttattggttc tagttggttc tttgaaggga atactgacaa
    50401 gggaatcaaa aatattttgg atagtctagg tgatcaagaa gaaattgaag tagtaattaa
    50461 ttcaaacggt ggagacgtat ttcaagggat tgctattggg aacttactta agtcaaataa
    50521 agcaaaagtt aacgttgtga ttaacggctt agccgctagt gctgcttcaa ttatcgcaat
    50581 ggctggcgat actataaaaa tctacaacaa tgcacaattg atgattcacc gcgcttccac
    50641 atacggagaa ggaaatgtcg atgacttccg tacgattgct gaccaactgg aatcaattga
    50701 taaatcggta aaggcttcat ataaaacacg attcaatggc acagatgaag cattgcaaga
    50761 acttcttgaa aaagaatcgt ttatggatgc agaaacagct ttgagttatg gattggtcga
    50821 tgaaattatc gatgcagaaa atagctcagg tactgaagct aaaaaagaac aaagcgttga
    50881 agaaattttg aatgacgtta aagaaaaaag agcagaaaaa attgctgcat ttacagcagc
    50941 attaaataaa acatttggac aaggagatgt aaaataatga cagttaaaaa tttaaaaggt
    51001 gtaacagctg caagcgacca attgatgaaa gcttttaaag atggtaacga agaatctttt
    51061 agcgcagcta tggtaagctt atctaaggaa attcaggata aaattttaga agaagcaaca
    51121 gcaaaaaatc aagatcaatt agtattaatg aaccgtggtc agcgtgtatt aactacacaa
    51181 gaaacaaaat tctataacga agtagtgaaa aacgaaggtt ttgcaggggt cgaagaatta
    51241 gtgccagcta ctgtatttga acgtgtattt gaagatttag aacaatctca tccactattg
    51301 caaaaaatta cttttgttaa cacaactggt gtaacagaat ggattgtgtc acgtggagtc
    51361 aatccagcat ggtggggtaa actttgcgaa gctgttaaaa aagttttaga taatggcttt
    51421 gatgtaatta acatgaagca gttcaagcta tcaggttata ttcctgtatg taaggcaatg
    51481 cttgatttag gtccagtatg gttagatcgt tatgtccgta ctgttttagt agaatcattg
    51541 agaattgcat tagaacaagc aattgttgat ggtactggta aagatatgcc agtcggaatg
    51601 atgcgtgaca tgagcaaaca aactagcgga gaatatgctg aaaaaaaagc agaacctatt
    51661 acagctttag atgctgtaac tatgggcggt ttgatggcgc gactatcaaa attcaatatc
    51721 gaaggtgtga atgatccgat ttatcgtaat gtgaatcctt ctgatgtggt cctaattgtg
    51781 aatccaactg attactggtc taaagtattc ccagctaaga ctgtactaac tgctaatgga
    51841 gaatacgtac aagtattgcc agtaccagtt tcagatttgc agtcaacggc tgtgccagaa
    51901 ggaaaagcag ttattggggt agcctcagat tacttcatgg gtgtaggatc tacactaaaa
    51961 attgaagctt cagatgaata ccattttgtt gaagacgaac gcatttatct agctaaacaa
    52021 tatgcaaacg gtcaacctaa acgtaacgat agtttcattg tattagatat tagcgctttg
    52081 ggaactacta ctacaactac aaaaccaaca accacaacaa ctacaacaca agcgtaggtg
    52141 atcagaatga agtatattct ttgtcagccg gcaatcaatc ggtttaaatg ggagcttgaa
    52201 gtttgtttaa ctaatctgaa gaaactagga atcaaagata tcgtattgct tttcagcaga
    52261 cacgatgatc agattcctat tttttttgag aaggaatatg gtgttgaagt tcatgtgtac
    52321 gatgatctgc gggacgacaa agagtatatt ccttcgatta aaccatattt atggtggaaa
    52381 tatttagaag aagatcattc gcgtgaggac gaccgatatt tctatatcga ttcggatgtc
    52441 attttcaata aaagaattaa tttgcgcaaa ttgccttcta aagatgatgt ttggtattgt
    52501 agcgactgct gtagttatct aagtcttgat tatattagaa gctgtgaaaa cggagaaaat
    52561 attctaaaag atatggcaaa cattgtaaat gttacagtag aatctttgga aactataaac
    52621 actaattcag gaggcgcaca gtgggttatt aaccgcccta aagcgaatta ttggaaaaag
    52681 gtttatctgg attctaatcg gctatatcgc taccttagag ggcaaaaaac aaatatacaa
    52741 atctggacag ccgagatgtg ggcacagctt tggaacatga tgtatttcaa tattggtcct
    52801 aaagttcacg aggaattaga cttttgtttt gctactgatc caatagaaaa agttaaagaa
    52861 gtaaaaatct tacacaatgc tggagtaaca acaaacgatg aagatttatt tttcaaaggg
    52921 agatacgtga cttctacgcc ttttgatgaa gatttatcat ttgtaaacaa gaaaaaatgc
    52981 tcttacgcat atgttaaagc aattaaggcg gtggttagat gacgcctgaa caagtgactg
    53041 aagaattgct aatagctgtg aaggataata tttacgttac ctggaacgaa gaagatgagt
    53101 caattaaaaa gatgatagct aaaaatgctg tttatcttca aagtaaagtg agtacaacac
    53161 tttctttttc tcctgaaagc ttagaatacg gattgctaat cgaaagatgt agatacgact
    53221 ggaatcgtgc tttagatgag tttgaacaaa atttcgctag tgagttatta ggtttcattc
    53281 aacattatgc gctacaagaa tatattgcag gtgatgtgaa tggcgaataa tcgtagactc
    53341 gaagaaacat tcaacgatgg ttggttaaag attttgacgc aaactaccaa aagaaatgaa
    53401 ctaggaaaaa agattggtgt agaagataca gaaatcactt ctttaaaatt tagaaatctt
    53461 tccatgagag atagtgatat aacagctatg gatgcgatgg gatcgaaatt aactaagaaa
    53521 gtaaagactc catttcatcc aatcgccaag aaatttaata aagatcaata ttttatcgta
    53581 atcgatagta tgcgttacaa cgttatctat gccgattacg ataattttta tatctatttt
    53641 tatcttgaaa gtgtgggtga atatggtgat tgataattct aaagaaaaag aacgtttaaa
    53701 taagcaaatt tctgctatca aaacttcctt agaagagcat tttggcctca aactctttca
    53761 agactccgta ggcgaggatg agctacctga tgattttaat tacttcattc tcgaaacagg
    53821 agaaatagaa atgatcactg agccaaaata tagcgtgggt caaaatctat atctaacttt
    53881 ctattcagaa aatagagaag atttaacagg agattcacta gatattattt cattgattca
    53941 aaatcgttcg attcgttttc agagaatgga tcccaatcat ttaaaactag agaaccaaga
    54001 tcgctatatc gatcaattgg tatttacgtt tagacgatta ttgaagagtg attgtcatgg
    54061 ctaaaaatag ttgggagcta aaaataaatg gacatgatga acttcttgtg cggatggaac
    54121 gctattcaag cgagagcgaa cgactgatta acgaagcatt gaaatcaaaa ggttcggcta
    54181 ttgcagtgga taggattaca gaaaaaattc ctgtttctga agcagattta agaagagggc
    54241 accaacacgc aaaaaatagt cgtccactta agactcaata cattaatttg ggtttcatca
    54301 ttagacctac aagaaaattt gagtatttaa aatatcctga tttggggata ggtacttcta
    54361 aaagaaatca gccggacgaa tttatgagaa gaggattagg tcttgcactt gatccaatta
    54421 cagaacttct gattcgtcaa ttcgataaat taaataaata ggaggaacaa caatggctaa
    54481 aacaacaaca gtaacaactt ttgacaatat aagtattaaa agaatttctt ttaattttaa
    54541 gaatgcaaca aatgcaattt caactgattg taatggacaa ttagatggcg aaacagaaat
    54601 gcagagaatt gtaaaaaaat gcggttcaac agaagtaaaa tcgaaatcta aaccaatcaa
    54661 tatgacggta acaattactg cacatgtacc gatggaagtt tatcgacgct tcaatgggtt
    54721 gaaacaagat gaacgtatta aatcgggaat ttactcttat ggtcctgatt ccgtagggga
    54781 agatttctct cttgctgcag agatcgtgga tgatttcgaa gaaaaaaata agctgattgg
    54841 tatgttagca tgcacttcga atacaggatt aacattctct attgaaaatg gtgcggatga
    54901 agtagctgcg ttagaactag aaacaaaagt tatgcaagat gaatttggta aattttatca
    54961 tgaagcaatt gttgcagaac ttgaagaaga cttaacagat caatggatga cgaatctatc
    55021 tgctgatgtg attaaaaaaa cttcagttgt gacaactacg gccactcaat cacagtaaaa
    55081 aaaacggagg tagcgaaatg aacgaagatt actcaaaaat tgaactaaac gatggaacaa
    55141 ttttgaattt agaacctaaa ctgaatatca agaaattatt gatgatcaat agagatttta
    55201 acacagacga gtttgcaaaa atgactgtgg gaaaaggatc catggatatt tctgttattc
    55261 aaggtgcaaa ggctgtgtat attgcttacc gccaagcgaa catgactgat tatatttcat
    55321 tcgatgaatt tatcgataaa tgggattttg atatggctac tgccagctat atttatcaat
    55381 tgatgatgtt caaacaagca cgcgatgctt atcaaaaaga atttgaaaaa gcaaataagg
    55441 aaaaaaagct tcaaaagtaa aaatgccaaa gctcttagtt gaaacgtggg tcgatgtcta
    55501 ttcgatgttg accgacgttt tttctatgcc ttcagatttg gttttaagcg atatctgttt
    55561 agatgacatt ttgcaaatgg cttacaacaa gagtgcttat gaaggatgga aaaactatgc
    55621 aataaaccaa tcccagaaaa actaaagaaa ggaggtaaaa aatggctaaa aagagaacag
    55681 aagcagaagt aacattcata gctaacgatg acggattgaa atctacgtta aaagaaatca
    55741 gtgctgaatt aactaaaaat agagcagaat taaaactaga acaagctcaa ttacaacaga
    55801 ctggttctga atcagacaag ttaggaagta aattatcttc tttagaaaag cagtatgaat
    55861 tacaaagtca aaaagttgaa gtaactagcc aacgtttagc caatgccaaa aaatattatg
    55921 gagaaaattc caccgaagtt cagaaacttg agagagaact gattaaccaa caaacagcgc
    55981 aacaacgttt gtcaaacgaa attgataaaa cgagtaatgc actagctcaa gcaaaaggcg
    56041 aaatacagac gtacgagtct acaatgcaac agttggatag tgaacaaaaa aatgttcaag
    56101 ctagtgcttc tctgattgaa tccgaataca aaaaatggca agcaactgct ggtcaatcag
    56161 cttctgaatc cgagaaatta gcgaaagccc aagaatatgt ttctcaacaa tctgaaaatg
    56221 cagagaaaac gatagatatc ctgagacgac agttagaagc tacacagtct gagtttggcg
    56281 ctacatccac agaagcaatg cagatggagg cgaagcttaa tgatgctgaa cgtgaatttg
    56341 aagagttagg acaagctgct aaaaatgtag atacaactaa cttggacgat atcggaagca
    56401 aaatagatat gaataatcta atggaagctt ctgacgtttt aagcgacatt ggcgataagc
    56461 ttacagaatt agggaaacaa gcagtggact ctgctaatag tgtaggtagt tcccagagta
    56521 aaatacaagc taattttggt ttgactaaac aagaggctga agaattaacg aatgtagcca
    56581 gagacattta ttataaaggt tttggagaat cgttagatca gtccacagat gcattgattt
    56641 tggtaaagcg taatttaggc gatttaaata atcaagattt acaaaatatc acggaacaag
    56701 ctatggtcct agaaaacacc atgggcgctg atatggatga aacgttacgt ggtgtaaatg
    56761 gcttaatggt caatttcggc ttgagcgctc aagatgcaat ggatttaatg gtttcgggta
    56821 ctcaaaacgg tttagataaa acgcacgaat taggcgacaa tatggcagaa tatagccaat
    56881 tatggagtca aatgggatat tcagctgatg aaacgttcgg aatgcttcaa aatggtttag
    56941 atgcgggtgc ttataacctt gataaagtca atgacttagt taaggaaatg ggaatatcgt
    57001 taacagatgg tcgatttgag caaaacatgg atatgtttag tgaaagtact agaaaagctt
    57061 ttgaagagtg gaaaaatggc ggaggaacac aaaaagacgt tattaattcc atgattcaag
    57121 attttagcaa tatggatggt caatacgacc aattaaataa agcttctaca atttggtctg
    57181 cacttggcga agataacgcg atgaaagttg tccaatcttt gactgatgtt aaccatacat
    57241 ttgatgatgt tagtggatct gcacaaaaaa tgaatgaaga ttctactact ccgttacaag
    57301 aattaaacgg aaaaatagct gaattaaagg attcattagc tcctataggc aacacaatca
    57361 tagatgcact cgaaccagta attgattttc taggaaagat ggctgatgcg tttaataatc
    57421 ttccacaacc agtacaggat tatgccgtag cgattggcgg attgactgct gcatttactt
    57481 tattaatgcc aataatagtt ggcttcatgg ctctaggtgg tcctactaca ttaataatag
    57541 gagcagttat tactgttatt gctggagtta tagcaattat aaaaaactgg ggcgcaatta
    57601 ctgactggtt taagggaata tggagtaaat tcactgattg gttgggtggt acttgggaaa
    57661 gtataaaaga aggtgcctca tcagtttggg atggagttaa agaaacctgg tctggatttg
    57721 tagattgggt tcaagatatt tggcaaggag tttctgattg gtttggagag ctatggagcg
    57781 gattagttga aggagcttcc aacatctggc aaggagtcca agagacttgg caagcattcg
    57841 ttgattgggt ttcaaatatt tggaacggag tcaaagaagt atggtcgatt atttgggcag
    57901 acattgtagg aattgttcaa ataccatgga ccttaataac gtcattgatt caagccggta
    57961 ttaatattat cgtgggtatt tttgatgtag ctggacagtt attaggcgca gcttggcaag
    58021 ctgtttggac acctatttct gatttcctta aaaacacttg ggatactatg acacaatggg
    58081 taagcatcgc ttggaatgga attgtaacta cattccatac tatatttgat ccagtagtgg
    58141 catggtggaa tggtatatgg acagctatta gtactacggc ttcaaatatt tggaattcaa
    58201 ttagtgcaac agcttctagt atttggaaca gtatcaagaa tacaatcact agcttggtac
    58261 aagcagctgc tacagtaatt caaaatattt ggtcaactgt atctagttgg ttaggtggaa
    58321 tttggaattc aatcagctct acagcatcaa atatctggaa tagcgtgact agtagtataa
    58381 gcaatgctat aaacgcagct aaaagtgcca ttcaaagtgt ttggaatagt atatcttcgt
    58441 ggatcagcgg aatttggaac ggtatcaaaa acactgcttt gaatctttgg aatggaatta
    58501 caagcactat tagctctaaa gtaaacgatg gaaaaaatgc aatttcaagc ggttggtcca
    58561 atctaacagg tattgtttcc gacatattca ataatgttaa aagtacaatt gctaacattt
    58621 gggaaggtat caaaaagact gttagcgctc caattgattg gatcagagat aaaatcagta
    58681 gtatctttga taatttgaat atttctatac cacatattcc gttaccacat tttaaattga
    58741 gcggagaatt caatccattg aaggggaaaa tcccaacgtt gggtgttgat tggtatgcga
    58801 aaggtagtgt gtttaattct ccgaatatta tcggtgtcgg cgaagcagga cctgaagcag
    58861 ttttaccttt gaaaagatct gtgctgcaag aaattggtga tcgtatcttg agtagcacat
    58921 cagtttcatc tagggcacaa acgattcaac ctgtgaataa ctacgaattc aatttcacaa
    58981 ttgatggtaa cgcagatgag gttactatga agcaaacaac tcaacaaatc attgatagca
    59041 ttacaaaagt tcaaaatgat aatgcttcgg catggcgtta aacaggagag tatttctcct
    59101 gtttttttag tattaaaaag gatgtgaaaa aatgactgat tgtatacatt ctataatcga
    59161 tggatttcct gattatttgc ataaattggc tttagctgaa agaccaacca taccttctcc
    59221 aaaaagacag agagttgaaa cttctgtttt aggaaggtta ggtggcttag tacaagatta
    59281 ctcgtttgaa gacatgtcgt ttacattgca ctataactat ttagaggatg tggaagacca
    59341 tcaagcgttc aagcaatcgt tttatatcat gcgtcattgg ttaaattatg caaagaaatt
    59401 agaattctct gatgatccca acgtctatta tgttatccag actatcgata ttggggatgc
    59461 agaaaacgat attgttgaat ggggagagtt cgatgtaaat attactgcga aaccattcgc
    59521 aagagttcaa gaagatgtac ctataaccgt agataaacca cagtcattta acttgctgaa
    59581 taatagttta gaagaaagtt ttccaaagat tatcatcact ccttcagcta cttcatgcca
    59641 gttcatctta aatgattatg tgtttagttt tgaaggctta gtagtaggaa ctgacgtagt
    59701 cattgatagt gatttgatgc tttgctacga agagcaatcg gacggagata ttttagatcg
    59761 gtccaacaaa atgaagacca tgcaatatcc gacattgcaa gtggatatta attattttaa
    59821 ttgtactggt ttgagcaaaa tacaaattta tcgtaatggg ttaaggtagg tgaaatagat
    59881 gatcgataat ttaataacta tttacgataa aaacgacgcg aataatttag ctgaacattt
    59941 atatgatacg caaggtttag gcgctttgtc agactggtta acagctactg ttagcaataa
    60001 attaaacgga gccgagatat ttcagggtac ttatccaata agcggaacta atgcagattt
    60061 gattgtagaa ggacgtatta ttcagtgtta tgtagatgaa aatcgagcaa aacagcgtct
    60121 acggatttat tatgcaaaga cttctgtaat aggcaatacg atagaagtaa aagctgaacc
    60181 tattttcaat gatataagaa aatcggtgtt gaataaatat gacagtggaa cagaaaagat
    60241 cactgctagt caggcatggc aaaacgcaaa aactttagcg aaaccagtta ttccttcaca
    60301 gttttctttc tcgtcattag tagatacgct tgctaatgtg aagatagaaa aggcgaattt
    60361 tttagaattc tttggtggaa aagagggatc tattctagat cgatttcatg gggaatttct
    60421 aaaagataat aacacattac gtcatgaaaa aaggctaggc acggatcata aaatcaaagc
    60481 gatttatact aaaaacttaa ctggtcttga cttagagatt gatgctcaaa gtgttttagt
    60541 tggagtttat ccattcatta gcagctcttc agaaggagaa gacgagatca ctctaccaga
    60601 agaagttatt ttcacggatt acgtggatga ttatcctgct ggatatgttt cttttgttga
    60661 ttttaaagac aaagcgactg atgtagccac attaagggaa gctgctaaag actggttgaa
    60721 aacaaacata gataaacaaa aaccacaagt gagtggttcg attgaattag taccattgag
    60781 gcatcaaaga ggctatgaaa aatttgttga tctagaaaaa gtttcgatgg gtgacggagt
    60841 agatgtgtat catccacagt taaaagtgaa tatgtcagcg agaattgtgg aatatacgtt
    60901 taatgttcta accaattcat acgataaatt agttgtagga aacgtcaaaa caaacttctt
    60961 agaaaacaca gagaataatg tcagcaattt gattaatgat gccattgatc aattgaaaaa
    61021 cggtggcgaa atcagtgatt tactcaatga tattgtagat catcaaactg atatgattac
    61081 tggtcaaaat ggtggttatg ttttattaga tcctaaagaa gcgcctagtc gtattttgat
    61141 tatggacaca ccagataaga ataccgcaag gaatgtttta caaatcaaca atgctggtat
    61201 tggtttttct aaaactggca ttaatggaac atatgaaacg gcatggacgt tagatggcgg
    61261 attcaatgcc tcgtttatta cggctggtga aatagtagga attactatta gaggtactac
    61321 attaattagt gatggtgctg attatagaac aagtattgct aatggcaaaa tgacttggta
    61381 ctcaaaaaaa gttaacaaag atattatgga gctagaagca cgtgattatg taagtgctga
    61441 tgccggtatt gtatcataca ccatgaaaac tggtggtggt ttcatgatta gaaatccaca
    61501 gggtaacttg gtttttagta cgtgggataa tggtaataac agaccgtttc tatcttttgg
    61561 tgcgcccaat ttcaggtata gcaatgctag ttatgtaact tctggcgacg gtagttcttt
    61621 aagcattaat ggtagtgcgg gtaactcatg ggaatttaag gtagctggta ggactatgaa
    61681 atttactagt gatggtatgc taacgttacc aggttgtttt tttggttcat gggaagatgg
    61741 gaaacttgct aggtttgaac aatcaacggt acaagtatat aaagatttta ctgttagagg
    61801 tactaaaaac tcaactgtac caacagaaca ttatggacaa cgactattga acgcttatga
    61861 aactccagaa tattatttcg ctgattatgg ggaagccgtt acaggtgacg atggtaaagt
    61921 tcgtgttgat attgacccca tgtttgctga aacagtaaat ctaagtcggt atatgacaca
    61981 tgtgacacct acagaactag ttttgtgtgc tgttactcat gaagatattg accatttcat
    62041 cattgaaact agtaagccaa acgtattagt tagatggaat ttagtggcac accgtctagg
    62101 gtatgaagat attagattaa aagaggatac agcatatgat agcacagtgc ttgaccaaaa
    62161 acgtttttaa aacgaagaca aggaggtata taaatggcta gcagtttata taatttggct
    62221 ttagatttca gcaaagaatt aaactacacc aaagctatta tggctcgtca aggtgataaa
    62281 gggattacgg tgacggttaa accgtttcta aatggcttgc agatggatac gagtggcgga
    62341 acatttactt taaaaggaac aacaccatct aaccgttacg tagataatgt tgcaactagt
    62401 gtaactagtg aagaagtcac gttttctctt gatggcacat ttatgagtga agcaggatat
    62461 tataaacact gctacgtaga atatagaaaa gacaatcaaa ttttaacaac gcaagatatc
    62521 atttttttct cactaggagt gtctgacatt tcgcaaggcc aagccgatga atatgtttcg
    62581 caattagaag agttgattcg aaagtacaac gaaacttttg atgcttttat ggctgaaatc
    62641 aaaggtagag tggatagctt aaatcaacag attactgatt taactggtca agctaaaacg
    62701 ctacaagaca agttagatgc tctgaaagaa gaaatttcta agttaggtaa cttacaagtg
    62761 atgtacagta acagcatcga cttcggggga tatgattata gcggtaagcc gaatttaatg
    62821 tccaaactaa aatcgagcga ttttaacgtt ggttaccacg ggtcactaac tttggataac
    62881 gaaaagttac attttacttc tgatggtaca ggaagcatta ttatgtttac gcgtattaat
    62941 acacctcagc ttactagtgg gaaaacctat actctgagtg cgaaagttcg atttgatgaa
    63001 ggaactacag gagctattga taaattacgt ttggtgtatc gtacatcacc aggagaaaag
    63061 atattattgg aagcaaatag tacaaatatt acaacagatg atgtagggaa agaaataaca
    63121 atcaaaggta cagctaacgt taattatcaa atcacaaatt tagatcgatt ttatatgagt
    63181 attagctttg ttgacaggga taaaataaat ggcggattta agttgtacga catcaaaatc
    63241 gaagaaggct caacagccac accgtaccag cctaatctac ttgatgcacc gtattatttg
    63301 agtaaggtgg ctttgggtga gaatctaatt aaaccagaat cacaacaacc agttactaat
    63361 agtaactatc ttattaacac ctataacatt aaaccaatgg taaaaggtaa gaagtatacc
    63421 atcacacttg aaggaactaa gccagcaaca caggttttta gaccattttt cacacgagca
    63481 acaggagatg catgggaggt tggtgactta caaccagtag aaggcttaac taatgtgtgg
    63541 tctaaaacat ttacagcagc agatgactca caccctacta ccccacaagt gcagatttat
    63601 caagtaccaa gcacaagtgt aggacaatgt acaattaagt ggttaaaact agaggaaggc
    63661 aatactcgaa ccccgaatat tagtgagtat aaatatcgtg gtactggtat gcgtgattca
    63721 aacaatccaa aagattatgt ttgggatcta gcaccagaat atgtcgaaga taatttggcc
    63781 acagatatta aaatttctga aattactggt aaagcaaaca attataccga tgggaaagta
    63841 tcggagatta attcgcagtt gactgcttca attaatgaag tagacaccac agctaaggat
    63901 gctcaaacaa aagcgaatgc taatgcgact gctatagatg aattagacaa taagatcgat
    63961 gaacgcatta atgatacagc tactaccaca ttaacagtta caaacgggaa taccggatca
    64021 gcaaagcttt atcgtgaagg aaaaacagtt tctatatatt ttgtggcttt aaacggaaaa
    64081 cgcagtggtg gaaatgattc aacgatacta acaattccag aaggctatcg gccaccaatt
    64141 agttttgagc aactggttgg ctcgatagac cgttctactt tgaacagtgc tcagttatct
    64201 attggtgcag atggagccat taaatggcga agaaactcaa gttatggatc ggattatacc
    64261 tttgcaatta cttacacgat ttagaaaagc gtgaatcgat atgaaggcag catatagacc
    64321 aattgaacct tacggattcg agcaaatcat tgtgaatgat gaagaacatt taccggaaga
    64381 atgcacagaa gtcgaaccac cgattccaaa ttggaaaccg aaattcaatt actgggaggg
    64441 aaataaatga aaaacatttg gaaatatgga cgtactggcg gagagtacgc aggaaaagta
    64501 ttggacgaca tgcttgtatc cgttccttac acagatcagc caccgcttga aggaattcgt
    64561 gctgatggcg aaccgttaac aatcgctgat caaatgtttg atcctaaatt gaatcaatgg
    64621 attattttag cgaacgcact agatcacaac gatttaaaca atctcaaagc aatgtatgag
    64681 tcgttagaaa atgagaacgg cgatttaaaa cagatcaatg ccaaactcat gctaagcgat
    64741 gtagcaatta aacaggaaaa tactgcattg aaagaaaaag cggatagttt agcacaaatc
    64801 aattcaaaaa tgatgcttgc ttcgttacaa aatagcaaag acatttcaga aattaaagag
    64861 caactaaatc cagcttcaaa gggaggtgag tagtatgttt agttttagcg atgtgaaaat
    64921 gatgtatgat tggggctgtt tcactaacga acaagtaatg gttttcgttc cgttgtgcat
    64981 tactgaagaa aaagcagata aaatcattag caaagaagag agcgcatctt aattgatgtg
    65041 ctttttattt tgattcaagg agttgtcaca tgattaattt aggggaatgg ggagcgatag
    65101 caggatcaat aaccgctatc gtttctttga ttttattagt aataaaacca attactgcat
    65161 ctttctcgaa gattactgag actctttcaa aagtaagtca caatttagat ttgctgacta
    65221 aagatttaga atcgagcaaa tcagatcgat tgatgattca tgaagaacta aagaaacacg
    65281 atgaaagatt agatacacat gcagaaaaat tggtagaaca cacgcaacaa attaaaactt
    65341 t
//
LOCUS       DACCCS010000007        64201 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.007, whole genome shotgun sequence.
ACCESSION   DACCCS010000007 DACCCS010000000
VERSION     DACCCS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.007"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(143..1534)
                     /locus_tag="IVK82_000491"
     CDS             complement(143..1534)
                     /locus_tag="IVK82_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP6391576.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1747..2133)
                     /locus_tag="IVK82_000492"
     CDS             complement(1747..2133)
                     /locus_tag="IVK82_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP6391577.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2189..2812)
                     /locus_tag="IVK82_000493"
     CDS             complement(2189..2812)
                     /locus_tag="IVK82_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP6391578.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2876..3544)
                     /locus_tag="IVK82_000494"
     CDS             complement(2876..3544)
                     /locus_tag="IVK82_000494"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP6391579.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3556..3996)
                     /locus_tag="IVK82_000495"
     CDS             complement(3556..3996)
                     /locus_tag="IVK82_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391580.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4055..4726)
                     /locus_tag="IVK82_000496"
     CDS             complement(4055..4726)
                     /locus_tag="IVK82_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6391581.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4911..5309)
                     /locus_tag="IVK82_000497"
     CDS             complement(4911..5309)
                     /locus_tag="IVK82_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP6391582.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5306..6079)
                     /locus_tag="IVK82_000498"
     CDS             complement(5306..6079)
                     /locus_tag="IVK82_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP6391583.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6076..6759)
                     /locus_tag="IVK82_000499"
     CDS             complement(6076..6759)
                     /locus_tag="IVK82_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP6391584.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6926..7813)
                     /locus_tag="IVK82_000500"
     CDS             complement(6926..7813)
                     /locus_tag="IVK82_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6391585.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7810..8679)
                     /locus_tag="IVK82_000501"
     CDS             complement(7810..8679)
                     /locus_tag="IVK82_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6391586.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8816..10102)
                     /locus_tag="IVK82_000502"
     CDS             complement(8816..10102)
                     /locus_tag="IVK82_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6391587.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10375..10953
                     /locus_tag="IVK82_000503"
     CDS             10375..10953
                     /locus_tag="IVK82_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP6391588.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10953..11882
                     /locus_tag="IVK82_000504"
     CDS             10953..11882
                     /locus_tag="IVK82_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6391589.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11879..13615
                     /locus_tag="IVK82_000505"
     CDS             11879..13615
                     /locus_tag="IVK82_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6391590.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13678..14514)
                     /gene="pheA"
                     /locus_tag="IVK82_000506"
     CDS             complement(13678..14514)
                     /gene="pheA"
                     /locus_tag="IVK82_000506"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP6391591.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14511..15020)
                     /locus_tag="IVK82_000507"
     CDS             complement(14511..15020)
                     /locus_tag="IVK82_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP6391592.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15013..16308)
                     /gene="aroA"
                     /locus_tag="IVK82_000508"
     CDS             complement(15013..16308)
                     /gene="aroA"
                     /locus_tag="IVK82_000508"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP6391593.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKKRSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16321..17412)
                     /locus_tag="IVK82_000509"
     CDS             complement(16321..17412)
                     /locus_tag="IVK82_000509"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP6391594.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17429..18595)
                     /gene="aroC"
                     /locus_tag="IVK82_000510"
     CDS             complement(17429..18595)
                     /gene="aroC"
                     /locus_tag="IVK82_000510"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP6391595.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18597..19661)
                     /gene="aroB"
                     /locus_tag="IVK82_000511"
     CDS             complement(18597..19661)
                     /gene="aroB"
                     /locus_tag="IVK82_000511"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP6391596.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19694..20716)
                     /gene="aroF"
                     /locus_tag="IVK82_000512"
     CDS             complement(19694..20716)
                     /gene="aroF"
                     /locus_tag="IVK82_000512"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP6391597.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21375..22349)
                     /locus_tag="IVK82_000513"
     CDS             complement(21375..22349)
                     /locus_tag="IVK82_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP6391598.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22330..24213)
                     /locus_tag="IVK82_000514"
     CDS             complement(22330..24213)
                     /locus_tag="IVK82_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP6391599.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24231..26456)
                     /locus_tag="IVK82_000515"
     CDS             complement(24231..26456)
                     /locus_tag="IVK82_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP6391600.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26571..27605
                     /locus_tag="IVK82_000516"
     CDS             26571..27605
                     /locus_tag="IVK82_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6391601.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGKAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27672..28685)
                     /locus_tag="IVK82_000517"
     CDS             complement(27672..28685)
                     /locus_tag="IVK82_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6391602.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28763..28951)
                     /locus_tag="IVK82_000518"
     CDS             complement(28763..28951)
                     /locus_tag="IVK82_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP6391603.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29391..29519)
                     /locus_tag="IVK82_000519"
     CDS             complement(29391..29519)
                     /locus_tag="IVK82_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP6391604.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29522..30895)
                     /gene="rlmD"
                     /locus_tag="IVK82_000520"
     CDS             complement(29522..30895)
                     /gene="rlmD"
                     /locus_tag="IVK82_000520"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP6391605.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31006..32061)
                     /locus_tag="IVK82_000521"
     CDS             complement(31006..32061)
                     /locus_tag="IVK82_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP6391606.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32071..33501)
                     /gene="gatB"
                     /locus_tag="IVK82_000522"
     CDS             complement(32071..33501)
                     /gene="gatB"
                     /locus_tag="IVK82_000522"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP6391607.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33498..34970)
                     /gene="gatA"
                     /locus_tag="IVK82_000523"
     CDS             complement(33498..34970)
                     /gene="gatA"
                     /locus_tag="IVK82_000523"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP6391608.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(34970..35275)
                     /gene="gatC"
                     /locus_tag="IVK82_000524"
     CDS             complement(34970..35275)
                     /gene="gatC"
                     /locus_tag="IVK82_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP6391609.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35381..37417)
                     /gene="ligA"
                     /locus_tag="IVK82_000525"
     CDS             complement(35381..37417)
                     /gene="ligA"
                     /locus_tag="IVK82_000525"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP6391610.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37434..39668)
                     /gene="pcrA"
                     /locus_tag="IVK82_000526"
     CDS             complement(37434..39668)
                     /gene="pcrA"
                     /locus_tag="IVK82_000526"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP6391611.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39823..41382
                     /locus_tag="IVK82_000527"
     CDS             39823..41382
                     /locus_tag="IVK82_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP6391612.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41493..43424)
                     /locus_tag="IVK82_000528"
     CDS             complement(41493..43424)
                     /locus_tag="IVK82_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP6391613.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43442..44356)
                     /gene="pfkB"
                     /locus_tag="IVK82_000529"
     CDS             complement(43442..44356)
                     /gene="pfkB"
                     /locus_tag="IVK82_000529"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP6391614.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44353..45105)
                     /locus_tag="IVK82_000530"
     CDS             complement(44353..45105)
                     /locus_tag="IVK82_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6391615.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45264..45473)
                     /locus_tag="IVK82_000531"
     CDS             complement(45264..45473)
                     /locus_tag="IVK82_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391616.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45484..46812)
                     /locus_tag="IVK82_000532"
     CDS             complement(45484..46812)
                     /locus_tag="IVK82_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391617.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(47177..47764)
                     /locus_tag="IVK82_000533"
     CDS             complement(47177..47764)
                     /locus_tag="IVK82_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP6391618.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(47847..49097)
                     /gene="clpX"
                     /locus_tag="IVK82_000534"
     CDS             complement(47847..49097)
                     /gene="clpX"
                     /locus_tag="IVK82_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP6391619.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(49344..50633)
                     /locus_tag="IVK82_000535"
     CDS             complement(49344..50633)
                     /locus_tag="IVK82_000535"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP6391620.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(50787..51719)
                     /locus_tag="IVK82_000536"
     CDS             complement(50787..51719)
                     /locus_tag="IVK82_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391621.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(51876..52535)
                     /locus_tag="IVK82_000537"
     CDS             complement(51876..52535)
                     /locus_tag="IVK82_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP6391622.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(52538..53563)
                     /gene="sppA"
                     /locus_tag="IVK82_000538"
     CDS             complement(52538..53563)
                     /gene="sppA"
                     /locus_tag="IVK82_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP6391623.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(53576..53866)
                     /locus_tag="IVK82_000539"
     CDS             complement(53576..53866)
                     /locus_tag="IVK82_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391624.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            54275..56080
                     /gene="glmS"
                     /locus_tag="IVK82_000540"
     CDS             54275..56080
                     /gene="glmS"
                     /locus_tag="IVK82_000540"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP6391625.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(56152..57507)
                     /locus_tag="IVK82_000541"
     CDS             complement(56152..57507)
                     /locus_tag="IVK82_000541"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP6391626.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(57531..58700)
                     /locus_tag="IVK82_000542"
     CDS             complement(57531..58700)
                     /locus_tag="IVK82_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391627.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(58697..59584)
                     /locus_tag="IVK82_000543"
     CDS             complement(58697..59584)
                     /locus_tag="IVK82_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP6391628.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(59773..63465)
                     /gene="nifJ"
                     /locus_tag="IVK82_000544"
     CDS             complement(59773..63465)
                     /gene="nifJ"
                     /locus_tag="IVK82_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP6391629.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            63664..64185
                     /locus_tag="IVK82_000545"
     CDS             63664..64185
                     /locus_tag="IVK82_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP6391630.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT        17837 a        13921 c        11463 g        20980 t
ORIGIN      
        1 ttttcacaac ctcttattca cggtgtttca gaagtagctt ctcggaaata agccgaaatt
       61 tcacaaaaat ttgagaaact atttttagga aacttcttct tatttcttaa agctgaccac
      121 ttctatcaca acctcttttt gactatgcat tcacatcgat cactttagaa tcatcattga
      181 tgacatcgcg atctaaatct ttgatgcgtg cacttgtcac gataccagct accatgctac
      241 cgctgacgtt tactgctgta cgtgccatat cgattaatgg ttcaacagag ataactaacc
      301 caacgattgc tactggcaag ttcattgccc ctaaaacgat cagtgaagca aatgttgcgc
      361 ccccaccgac tccagctaca ccaaaagaac tgattgttac aattgccaca agcatcaaaa
      421 taaattccaa actaaagaca ttcactccag ctgctggtgc gacgatcgtt gccaacatag
      481 ctggataaac gccagcacag ccgttttgtc caatagacaa accaaaactt gctgagaaat
      541 tagctgttgc atcatctact cctaaagctt ttgtctgagt ctcgatattc aatggcaaag
      601 ctccagcact tgaacgtgaa gtaaacgcaa agctcaatac cggaaaggct tttttgaaat
      661 agttgattgg attgactttt gcaccaatca aaatcagtga gtggacaatc aaaacagcaa
      721 gcaatgctac gtaagaagca acgacaaatt tacctaaatt gacgatagct gcaaaatcac
      781 ttgttgccaa aacgtttgtc atcaaagcaa atactccata aggtgtcaaa cgcaaaacaa
      841 gcgtcacgat ccgcataatg attttataca gactgtcgac taaatctgaa aagaatgctg
      901 cttcttttgg cgcttttctt ttcacgccta gataagcgat tccgacaaat gccgcgaaaa
      961 taacgactcc aatcgtactt gttgcacgac tacctgctaa atcagcaaaa acgtttttag
     1021 ggataaagga taaaatctgc tgtggaatcg ataagttttc aactgtttcc tgacgtgttg
     1081 ccagttcaga gattcgtgct gtttctgcag ctccttgtgt gaatttagct ccttggagtc
     1141 cgaacagcat tgttgaacca attccgatca atgctgcaat agctgttgtt ccaagtaaag
     1201 aagtcaatac agtcgcacta attttcccta gcttttcaga aactttcatt tttgtgaatg
     1261 caccaatgat cgaaataaac actaaaggca taactagcat ttgcaagaat gatacatatc
     1321 cttgacctac aatcccgatc cattccattg attgagtaac gactttgtcg tctacaccaa
     1381 agatcaattg aagcagaccg ccaaagacga taccaactcc taatgctgta aagacgcgtg
     1441 ttgaaaattt tgtatggcgc ttttgcatac gatagaacac atataataat gccgcaaaaa
     1501 ctagaaccac taaaactgtg atgagtgttg tcatttttct cctcctcata ttattaatga
     1561 caagtccatc gaaggaagta aaggttcccg agaatgattc ttcaaccatc ctcctgaaaa
     1621 aatttttttc agtgactttt atattttaga cgatttttat cgaaatgaca ttctttttgc
     1681 ttacaattag taataaaaaa acatacctgt gatagcttta atctatcgca ggtatgtttt
     1741 tactgtctat tcttgttttt ctgattgaaa attcaatttt accccaggct gatctaaggc
     1801 gatctctgtc acctcataag acattcggcg tacgatatcg aataacggtg ctactagctc
     1861 gtccacttct tcttgtgtga catcattttg accttcaatt ttccgattga ggatatgatt
     1921 gatctgactc acacttccag ataaaacata tgtatcaaac actaaacgga actctaagcg
     1981 tgctcctacg atgctatgtt ctttcggata gttttcgatc gtttctttta aaggcgagaa
     2041 gcctactttc aaatctgttt ctacttcttg gttcggcaat ctcatatcat aatgaaatgc
     2101 ttctacaact tctttttgtc ttctgatttc catataattt tatcactcct ttatgaaaat
     2161 tataacatat ctcctttgaa aggggatact actttttaat gatgctctgc aaggtaagga
     2221 tttcattcag atttcctacc acataatctg ccgggatctc acccagaaaa tgatcgatgt
     2281 cgtaaagaca agtagcgact cctgcatttt tccctgcttc gatatctaaa cgacgatcgc
     2341 cgatcatcat cgtgtctgtt ctttctaaat gaaaagtatc gatcaaataa tttaaagatg
     2401 ctggatcagg tttacgagga aagtcctgat cgatccctac tacttcctca atcagatgtg
     2461 ccaatcgatg ttcttttaac aggccccatg ttgattctgt caaacggtgg gtcaggatga
     2521 aatgacgacc gccgttgtct tttagcattt ccagtacttg tttcgtttct ggaaatggac
     2581 gggaaacttc cgtactttct ttttcatatc ggtgaaataa ttcattaaac tctctggcat
     2641 tcaagtttga ttcattgatc aaataactag tagaatactt tttcattttg aagtagattt
     2701 cttttttgtc catactgata ccaaaatctt ttagtgcttg ccatgcacca tctaccattg
     2761 cgggatacgt atcaaacaat gtaccatcaa agtcccatat ataatttttc atgttccttt
     2821 tgttccattc ctttgcttct tttttctatc tctcactttt agataaaggt gttctttaaa
     2881 gtattttttt gtccagaatt gtcaaatcat ctgctaatgt atactggatt ggttgtgcat
     2941 ttgggatatc gatcgtatcc atttgatctt caggaacatc ttctaaatat tttgtcagtg
     3001 ctcggatgct attaccatga cccacaacta gtaaatcttg ccgatctttt agttttggtg
     3061 ctagatgatc ttgccataag ggaatgatcc gattcactgt catttccaga ctttctccgt
     3121 atgggatcaa atgcggatcc aagtgatcat atctgcggtc aaatctattt tctgcggtta
     3181 acggcggaac ttccgtatat cctctgcgcc atgtttttac ttgatctttt ccgtatattt
     3241 ctttcatctt atctttgttt ttacccacaa gatccccata atgccgttca tttagacgcc
     3301 atgtttttat tataggaagc cataactgac ccatttcttc tagtgcgact tgtccggtaa
     3361 gaatcgcacg tttcaaaaca gaggtataaa cggcatcgaa ttgaagtccg gcttcaagca
     3421 ttttttgacc ggcaactgct gcttgttctt ttcctttttc tgttaaggcg acatctagcc
     3481 atcctgtcca gtaattttca aaatttgctt cgctttcgcc atgacgtaat aatactaatt
     3541 tcattctttt ctcccttaaa cattcgtttc gaattttata ccacatgttt tgcatgtcac
     3601 acgtattcgt ccttttcctt taggtgcacg ctgcttttga tgacaattcg ggcactcaaa
     3661 aaaagtataa ctttttcgat ccattttgac ttgctttttg ttttttatct gatgatattt
     3721 ttctttgact ctatcaagat atttcaaata acgttgattc tcatttagtc tggggtagat
     3781 tttttttgat aggaaacgaa atatcatcca tccgaaaaag acgatgaaca acaattgtcc
     3841 gattctatag ggcaaccaac gacttagcaa actaaagaca agcgcagcga tcaatagacc
     3901 gcgattcaac tgatcaaatt tcgcatatct tccctgcata agtttgtgat agttctgctg
     3961 aaatcgtatt agcctttcta accatgtttg catcatatta tctcctccat catctaatat
     4021 ttattatgac aaaaaaatcc aaaaagaaaa agccttaatt ggccttttcc attgcaactt
     4081 ggtgaaggac attccgattt ctggtaaagt caatcacaat cactttcttt ttatcttctc
     4141 tgttgactac tcgaaatgtg tccgtcaatg gaagaaacag cttcttagat ggtagctgtt
     4201 gcatacgtat aaaacgatgg atcatcgtaa tcaattgagg aaattgaata gacgtgtgaa
     4261 gcagacctgg atattttttc aacagctgtg tcgttttgat cagattccgt tttttactga
     4321 tcacttttaa aaagcttttt agaatctttt gctgacgtct aaaaatagaa aaagaatttt
     4381 cgtcatctat ttgataacgc atttgttttt gcagttctgc tgcagtcaga tggatcttcc
     4441 catcgtttgt atcgatcatt aaaccttctg gaaacagttc aaccactgct cgatcccaag
     4501 attcaagaga aagctcgata taatctttta ctggtaattc caattctacc ttaaaaaagt
     4561 gcaaaacagc cgacagccct tctgcttgat agatttctcc taatgttagc ccctctagat
     4621 ggaactctgg tgaaaaatgc agcaatgttt tattttcctg caattgaaca tatagaaatg
     4681 tagatcgctc aatcagagta tcttttaaaa caactaaaaa attcattttt ctcccccttt
     4741 tttcatcatt tttaaaatag catattttga aaagaaagtc tgatgcttag agtagaattt
     4801 cataaaaatt tactatttca taagtattca cgagaaaaat acaaacaaaa aagcggaaat
     4861 aacgctcatc cgccttcttg tttgtgttta tttcctcttc aaacgcttgt ctatgattca
     4921 gacttttctc tatcgttatt ttcatctaaa agttcttgca ttccgataat ttctcgatat
     4981 tgatgaatct tagtgatacc aatgactttg tctacatcaa tactaaaaca aataccatta
     5041 cctggaatat cgatgttgat gccttgacca atcgcatcca ttactttatt cgtcaaacta
     5101 tcgggtacta atgtcaacac aatatctttt tctggcgaaa tgtccaaacc taaaaatttc
     5161 cctgaatcat gtacaccaga acctcttcca tgtaagatcg ttccgcctgc tgcacctgct
     5221 tcttttgaaa agtcgataac atcttcgccc attcctccgt caacgatcgt cacaatcatg
     5281 tgaagattca atgctacaag atctttcatt tgctttcccc caatttttgc aatctttctt
     5341 tttcgattct gtctttttca tcttttcgtt tataaatata tcctagtact aaagtggata
     5401 atattggtgt caatgcaatc attgcaacca caccaaaccc gtcaatgatc ggatctcttc
     5461 cttcaatttg tgcagcgatt gccacgctca ttgataaaat aaatgtcgaa cacatcgggc
     5521 ccgttgctac tccaccgtta tcaaaagcca ttgctaaaaa gatcttgtcc acttttcgtc
     5581 ctaaaataaa tgctagcccg tatcctggga tcaagaaata ataaagagag aatccgacga
     5641 gcaaacgcca catagacaat cctactgctg cgccgatccc aattgaaatt accatcagca
     5701 tgattttcga tcggattctc ccctcgctca attcttcaac ctgtttgatc attacatgaa
     5761 tcgccggctc agcaaaccct actaagaacc ccattaaaaa gccaagggga accagtatat
     5821 aattgtaagg caatcccgca atagcactgc ctaaatgctg tcctaccggt acataagcca
     5881 tattgactcc gtgcaggaaa aggatcaacc caatagtcgt tatcaagaaa cctttcataa
     5941 tgtcaaagaa acgctttttg cgcatacgaa aactaatgat attcattaaa acaaatagga
     6001 ttacgatagg tgccactgcc aaaatgactt ccatggtaac gatttcgatt ccatctatca
     6061 cttgctgcca gaaactcatt ggaagatcac ccccaaaatc atcacggcaa gaatcggccc
     6121 taacgaagca ataccgacca ttccaaaact gtcattttca tttttctttt gtttgatcat
     6181 actagtcatc cctccggcaa gggccaagat aaatggaaca gttactggac cagtagtcac
     6241 tccccccgaa tcaaaagcaa tcggcataaa ttcggaactg gtgaaaaatg aggcgatcaa
     6301 aacgcctatg tagcctatag ccatcaattg ataataggat agcttgaata cgacccgaag
     6361 cagcgcaaag gctagaaata ctccagtgcc gacgctgacg atcccaatca gcaacactct
     6421 gccaatctct ccgtcagata tatcatatac ttgttgtccc aaaacttgca cggaaggttc
     6481 agcaatcgtg atcacgatcc cgatagcaaa tcccaaactg accaaaacgg tcaaactttt
     6541 ctttttgatc atatatttcc ctactagatc tccgacttcc atcatagaat aatctgctcc
     6601 aaacaaaaag agcgtcattc cgatcatcat gattgccgca ccaaccagaa atgaagcgag
     6661 gtcttctcct tctaacggtg caaaaacaaa cgttaaaatg acaatcagga cagtcatcgg
     6721 caatatagca gcaatcactt ctttaaaatt ttttagcatg tatcctcctc ctttactctt
     6781 taagtttacc ttaaaatgaa agagttttcg agcacaaact taaaacgtta tcatttaatt
     6841 tttctcaaca taaaataaca cacgtcccaa aagtatcttt ttacttggaa aaatgacttt
     6901 gaaggaacgc gcttattgtg tttttttatt ttacagagcc gatcattcct tgaacgaatt
     6961 gcttttgcaa aacaaaaaat acgatggcag ttggtaaagt agcgatgact aatccagcca
     7021 tgatcattcc atagtctggt gtataggaag cacccatagc agataaaaca agcggaaccg
     7081 tccgtttatc aggagattgc aaagctacta agggccataa ataattgttc cagctactca
     7141 tgaatgtgat gattgcagcc gcggcatagg tatttttagc cgtcggcata tacactctaa
     7201 aaaaaatact tagttctttt agaccgtcta atcgagcggc ttctactaga tcttttggaa
     7261 aagatttaac attttgtcga aagaagaaga tcaaaaaagc agtgctaaca gatggcagga
     7321 tgacaaccaa gaaactgttg attcccaaaa ccgttccgtt caattgtgaa aacattcgat
     7381 ataaagggat catcaatgcc gcaaaaggaa ccatcatcga tagcagtaga atattgaaaa
     7441 ttaggtcttt tcgtttactt tgataaatct caaatccata tcctgccatt gacgaaatca
     7501 gcaatgcaaa cacagtggtc accaaagcga tcatcgctga attccaaagg gaacgagtaa
     7561 aattcagctc attggaaaac aaattttgaa aattgatgat caagttactg ccaatcttta
     7621 tttttcccga tgtgatatcc acgggtgtat tcgtcattcc caaaaccatc cagacaaaag
     7681 ggaaaatgga aataaatgat aggatgctta atgctgtata ctttaatact gacccaattg
     7741 tcgaacgaag ttctaatgct tgttttttga cgttggggcg ttcgattgtt tctttttcta
     7801 ccatattttt catcgacttt ctcctcctac tttcgtttgg attatcgaca atatcacaat
     7861 aaaggaaaca ataacaaagg aaactgctgc cgcatatcca aaattaggcg tgaatttata
     7921 actcaaatta tagatgtatt gagatagggt ggtcgttgca tttgctggtc ccccgccagt
     7981 aatattttgt acttcatcaa aaagctgcaa tgtcccgatc gtcgaagtaa tggaagtaaa
     8041 caaaatgatt ggttttaaat ttgggatcgt cactttgata aactgctgcc atttagaagc
     8101 accgtcaata gatgctgctt cataaatctc cgaatcaata ttttgcatgc ctgaaaggaa
     8161 aaaaatcata ttgtatccag tccaacgcca agtgatcgaa agaatgatca gaactctcgc
     8221 ccaaattgga tgagtcaacc agccaatcgg tgtgtcaatc ataccaatgc ccaacaacaa
     8281 gttgtttaca aggccagatt gagaaaatag acttttcatg atcagagaat aactgaccat
     8341 cgaagtgata catggaagaa agatagcggt tcgaaacaac cctttatatt tcaacttttt
     8401 atcatttaaa aggttggaaa taaaaagtgc tagaaacaac atgatcggta cttgtacaag
     8461 taaatacagg aatgtattaa aaaaagcttt tttaaaagtt gcatctgtca acatccgctg
     8521 ataattacct aacccgttaa atgtcatatt gacaggggga ccactttgaa aagacgtgat
     8581 caaagccaaa accatcggaa taaaaaccat gattgatatc aaaatgactg gcaagattaa
     8641 aaaagcatgc ccgtttttga ataaatcttt ctgcttcatt cttttccctc caattctatc
     8701 attgattatc tacatgaaaa gacaagaaag acaatatctg taaggcagac gtgatctttt
     8761 ttgtcttttc gtttgtctga tgaaaaagcg aagaaattca cttttttacc accgatcaat
     8821 tagccaattg tgcttctact tgtttttgtg tatctgctag taccttgtct gtctcttctc
     8881 cattgatgat tcgatgaaga gcttcagcta cgactgcttc aattgcatag gtctcatgtc
     8941 cgtaattcac ttctggtatt tcagatgtcc atgttgaaaa atcgtcaaag atcttttgac
     9001 cactgtaaaa ttcagaagct tcttgataag cttcttgttc attcgcgcta agcatcgtag
     9061 atactagtcc gatttctttc gcaagagtgc ccatcagttc ttcattcgaa gcaaacgttg
     9121 cttctaggaa atctttcgct gaatcttctc cagctactcc tttgatcaca taccagccag
     9181 cgccgccaag gttagaagct tgcgcttttt gcagattttc tggaagtgca ggtattggtg
     9241 caatcttcca ttttccagat tggttttctg ctccttggat cgttgaagaa taccaagcgc
     9301 cagttgggct agaagctact gctcctgatt gtatcgcagt tactccagca ttccagtcag
     9361 aggtttgttc gactagatct tctttcaaaa gagtggcaaa caattcaaga ccatacttta
     9421 aagatgcatt gtcttggatc gtgactgtct ccccatcttc agatgtatac cattctcctg
     9481 cctgttgcat aatcatcctt actcgaccaa ggtcagaagg attgacttca gcgattttct
     9541 ttcccgtctt ttctttgaca tctcgcgcta cttgaatata ctcgtcccac ttcagattct
     9601 ccatatcctc ttctgtataa cctgcttctg ccatcagatc cgtacgatag aagtttgctg
     9661 ttacgcccga atcgaaagga acaccgtata ttttatctcc tactttgttt accgcaaatt
     9721 tatacggtgc aaaatgatct tcctttacaa tagcagacaa attagcgaat gcatctggat
     9781 aagaagtcaa atagccttgt atcctataat cttcaatcaa gacgatattg ggcaatcctt
     9841 ctgtattccc gctcgctaaa gcggtgttca gtttctgaac gatatcatcc tgagacatcg
     9901 taacaacttc tatctctgtt tcttcgttct cataaacttt ttttgcttca ttcactgctt
     9961 tgatattgaa tgtctcatcc cacgcccaga cagtgacttt ttcttctttg tctgctgtac
    10021 tactagcact tccgcatcct gttaagaaac ctgatgctgc cgctaaacaa acgactccag
    10081 caattaactt tctgatgttc atttttatcc gctccttttc catccattag ttgtttacgt
    10141 ttgctacatt catcatacca aaatttgaaa acggtttctt tattattcag atacttcaaa
    10201 aatttctcct aaaataataa aaaaatacaa atcaactatg acaaaaggaa taatcttagc
    10261 atctagacca aagatcgaaa tggtgaggtt tctttaaaat aaaaatatcc ttgcattttc
    10321 tcctagaaat aagtattttt aaattacaag actattctta aggcgggtaa aaaaatgcca
    10381 atacgcgtaa ttcttatcga tgatgaacag acgattttag atggaatgaa acagttgatc
    10441 aattggtctg agcacgggtt tcaattaata gggacttttt ccagtccgct gaaagcgctt
    10501 gatttttctt tggtacatgc aatcgatatc gtggtcacag atgtaatgat gcctgaatta
    10561 aatggaatcg aattatcgcg gctattaaaa cagcaacaac ctgaaacaca gatccttatc
    10621 ctctctagct atgatgaatt tgatatggtt aaagattctt ttaaagaagg cgtcgctgat
    10681 tatcttttaa agcctcgatt gagtcctgat ttttttctaa attccttaac tgccgtttca
    10741 caaaaaatca aaaaaaagcc caacaaaagc ccaatcacca atcgttatga gcagatctgc
    10801 gagaatcttt cacagcagat tgctggacaa actcgtccat tgcttgaccc tcttcttttt
    10861 gaacacacct tgtttttctg ttatataccg aaaaacggcc attcagttca aaacaaggct
    10921 ctaaacaaaa acagtcgatc acccattcat tgatggatag cgacgattcg tttactattt
    10981 atcctgtttc taccttttcg gaagaatccg gctatgtaat caatactgat tccaaagata
    11041 cacttaggaa gtttgttcaa aagtttcgtc aagaagtttt tccaggtgat ttttttgttg
    11101 tttctgattc ttttgcaatc caagatatca atgaaatatt ccaagagatc agaaattgca
    11161 ccaaaggaca acgtttctat cataagggaa agaaccttgc ttatcagcat gaacttcttc
    11221 cgttattaac caaccacgag cagcaagcaa gtagttattt aacaaaaatc gttcataaag
    11281 actacgcttt agctgtcaaa gaacttgaga atttcttcac gacgatattg gaacagccta
    11341 ttgatccagc ttatttgaaa catgaggcaa tcaatcgttt ctatgcatta ctcaacgctt
    11401 tggctgacga gcatgttggg aatgaagaac tttctttgct caagatcgcc cttccttctt
    11461 tattagccga tgctgagtat ttcgaagatt tttctattct tgtcaaagaa acacttgaga
    11521 aactcaccca cttttctgca aatgcaaaaa aagacaattt cgatatcttg actttgatct
    11581 acgagttcgt ccaggaaaat tatgagaaag aaatcaattt acaactttta tccgaaaaat
    11641 atcatttttc atatagctat ctctcagcca tctttactga aaaatttggt atcaactttt
    11701 caaaatatct aaaaaaagtc cgtatcagta aagccaagct ctttttgacg gaaacacggc
    11761 ttactttgac ggaaatctgt gagaaagtcg gctataccga actgggttat ttctccagag
    11821 tttttaaaga agagacagga atgacacctt ctcaataccg taaaggaact tttgtaaaat
    11881 gagaaaaaaa atcaaagagc atgaactctt tgccaaactt ctgttcattt tgttcatcgg
    11941 tctttttctg caagccatcg tcatttcttt gtttatttat catcgatcaa gggatgccta
    12001 tatccagctg ttcaatcaat cgaatgatgt ggtactaaaa aagattcaat ctgaatttga
    12061 aagtttgaat gatacaatcg agaatacatt agccgctttt gattctaatc cagctgtcaa
    12121 aagctatttt tccaatgatc cagcccaaca tatggaacag cttcagcaac taagaacaat
    12181 ccaaaaaatg aatgactctc tttcaaaaat ccatccaatg atcgattacg atgtattgat
    12241 ctttggagaa aatggaagaa cttttgtcgg aaacgacatg ctcactgccg tatctgccga
    12301 ttcttttttt caatctgcga ttgctcagag agtgaatgaa cgtgctgctg atacacaaat
    12361 gctttttgca caccacggat tgactcttcg cgataagaaa gcacccagcg tcttctttgt
    12421 caaaaaactg aaaaatacat tgaatcacgt ttacggatat gctgttttat cgatctcatc
    12481 caagcaacta gcgaatcttt ttcaatctgt agtaaatcct gagatatcga agatctcctt
    12541 catgaacgaa gaacagacga tcatcgcatc aaatgaagaa aatacaattg ggagccgatc
    12601 aatgattttc aaaaaactaa cagtaggtga aacaacgatt tcaaatggac agcagttgac
    12661 ccgtcttcct ctgtaccgcc aggattgtac attactcagt caagtcgatc ttcattcttt
    12721 atctaaccaa atgggcgtga tttttccgat tattttgtat aatatcgtga cacttgcttt
    12781 tgctggctca ttggtattta tgtatttgga tcgacacaca aaatcgatct accgcttgat
    12841 tgattcgcta aaacggctag aggaagatgg cttaaaagct actgttcctg tacaaggaag
    12901 ccatgaagtc caattgctag ggaatacgat caatcaattg ctaagtaatg ctcagcatca
    12961 ttatgaacag acgatccaag acgaaaagaa gaaacgttcg cttgaaatcc aaactatgca
    13021 agcacagata cagccccatt ttatttataa tacgctgact agcttgaaat ttttgatttg
    13081 gcagaaaaaa acagatgaag cagttcaagg aatcgacagt ttcattactt tactaagaag
    13141 tacgattggg aaaaaagaag aagtgattcc agtcaaggaa gaaatcaaaa gcgtacaaag
    13201 ttacatcgat cttttgagta tgcgatatgg cgaaagaatc tcggctaaaa taatgattcc
    13261 tgatgaactg cagtcattat ctatgccgaa tatgatcctt caaccaatta tcgaaaacgc
    13321 ctatcttcat gcttttcaca ataaaacgca aggattcatc acggtgtacg gcaaaacaaa
    13381 aaatcagctt cttttattcg aagtgattga taacggcgat ggcttcgata ctgaaaatac
    13441 caaacaaaaa aatgatcatt tttcaggaat cggcatcagc catgtagatg agcggatacg
    13501 cctaatgtat ggcgatgcat atggcttaac gatccaatca gtgattggga caggaacgac
    13561 tgtaacgatt tgtctaccgc ttcttcctaa aaatcaatca gaatatcaaa aataaagaat
    13621 agccaagcgc tgattttttt attttaagtt aaataaatca atcagtattc ttgactttca
    13681 tctacgcgtc tcttctactg tcagcactgg ataacagccc aatatttgac tttctcctcc
    13741 gagcatcttg atttcttgga gcgcattctc tactaatgtc attggacggt ccaacaacaa
    13801 atcgatcacg aaaaaatatt ctcccaagct agttttcaat ggtcgggatt caatcttact
    13861 taagttgatt tcccgccagc caaaagctgc cagcatttta tgaagcatcc ctggccgatt
    13921 agcaggtagt gtcaagatga cactcatttt tactggagct gactgctggc tggtcatctt
    13981 tcggtcaccg acgatccaaa aacgtgtctg gttcaactca ttatcttgga tattctcagc
    14041 taagatctcc aatccaacat gctgtgctgt ttctaatgaa gcaatcgctg ctgcgtcttc
    14101 tttcggatgt tctgctacat acattgctgc agcagttgtt gattctgttg ctactaaagg
    14161 aacattagga taatgcgttt ctaaaaactg ttgtgattgt gctagtgctt gtggatgtga
    14221 aaggattttt gtaatctttg ttgctggatt gcctaaaagc tgctgcttga taggaagaac
    14281 gatttcactt ttaacttcga cttctggatg tttggaaaga agatcgattg tgtgatgaac
    14341 agctccttcc aatgaattct ctacaggaac gacagccaga tcgacttgtt tgcgaaacaa
    14401 agcgcgcaaa caaacaggga tggaagcata cgcagtcaat tgttctgtag gaaatagctg
    14461 acaagccgcc tgataagtga atgaactttc tggccctaga tagctgacgt tcatatgctt
    14521 ttgatccttt ctataatggt atcgatgatc ttttctggtg tttgattggt cgtgttgatt
    14581 cgatgtgtcg cactttcttc ataccaagaa agtcgttgtg ccaataatgc ttttatttct
    14641 tctcttgttt ttccaagagc taaaggtcga atagacgttt catcttgttg gatacgatca
    14701 actagacagt ccgcttctgc ctctaagtaa agaacaatcg gctgtgtttt taaaaaacgc
    14761 cgattttctg cactttggac aatccctcca ccagtcgcga taattttgtc ttcatttgac
    14821 agctttctta aaatatctga ctccactttt cgaaagcttt tttcaccgta atagtgaaaa
    14881 tattcggcaa tcgacaattg gatatgttcc tcaatctttg tatctaaatc tacatactct
    14941 ttttgtaaac attgagctaa ccctttaccg attgttgttt ttccagcgcc catgaaacca
    15001 atcagcaaaa tgctagccat gagcttctcc tcctgacaca agttctgcta aatcgtcaaa
    15061 aaatgccgga taagagattg aaacagcttc agctccttct agttcgcaag gtccatcagc
    15121 tatcaaagca gctacttgca gcatcatacc gattcgatga tcgcctctgc tggacacttt
    15181 tgcgccatgc agaggtgtcg gcccatggat gatcagaccg tctgctgtcg cttttatctt
    15241 tgctcccatt ttctgtaatt cttctgccac cgcatcaatt ctgttggttt cttttacttt
    15301 taattcttct gcgtctttga tgatcgtgat tccttctgct tgagtagcca ctaaagctag
    15361 aatgggcaat tcatcaatca gacgcggaat gatttctcca ccaatcgttg ctttcttcaa
    15421 atgactcgtc ttgacactta aatcagccga ttggttctgt tcattgtgat tcgtctgggt
    15481 aatcgctgcg cccatctcct ccaacacatc taagatacca gttcttgttg gattgacccc
    15541 gacatttttt aacaacagtt ggctttccgg aacaagaagt cctgctgcta gaaagaaagc
    15601 agctgatgaa atatcacctg gaacagttat ctgttgacca gttaattttt gcggtccggt
    15661 cactatgatc gttttatctt ccactgtgat ccttccgcca aattgacgaa tcatttcttc
    15721 cgtatggtta cgggaacgtt ttttttcaac gattacggat gtgccttctg cttgtaatgc
    15781 cgcaaacagc aaagcagatt tgacttgcgc actggctaca ggcatattgt atcggatagg
    15841 tgataaagag cgggttcctg tcacagtgat tggtagatac tggtcatttt cttctccttg
    15901 caagtcagcg cccatctcgc gtaaaggttc catcactcgc cccattggac gtttagacaa
    15961 cgaagcatcc ccgaacagag tagtcgtaaa aggctgtcca gccaaaatac ccatcaataa
    16021 acgtatggtc gtaccggagt ttcccatgtc aattgattcg gttggttgga caaatgagtg
    16081 gctaccgcga ccatgaatct tgatcacctc ttcctcttct tcgatctcga cacctaattg
    16141 ttggaaggca tgtaaagtac tcagacaatc ttctgcgcgc aaaaaatttt gtatagtcgt
    16201 cgttccttca gcaattgctc caaacatgat gctgcgatgg gaaattgatt tatctgctgg
    16261 tatcctaaca gtccctctta atccatgtat ttgttgtaat aattgcattt atccagcctc
    16321 ctatttgatc cagcaatgat aactggtatt agacataatg attctttttg cttgtaacaa
    16381 atcctgttgg tttttaaaac tgatttgcag tattcctaaa atatcttccc tggtctcttg
    16441 gatcttcaaa ttaatcaaag aaactcccgc ttttccgatc agtccagtta cttctgcaat
    16501 aacgccaggt tcatctggca catcgataaa taaatcaaaa aaggcaggaa tcgcgccttg
    16561 cttatggaca ggcaactggt ctcgactttc ttttgcccga ttgaaaaaag aaaagatttc
    16621 cgattggttc tcagtcatga tccattctgc tatttgtgtc atttcttgtt gccaatctgc
    16681 caatcgttcc aataatatct gcttattgct caacaaaata tctgtccaca tgacaggatc
    16741 tgaggaagcg atcctagtga tatctcgaaa gcctcctgct gccagttgtt tggctcgcgg
    16801 atactcttga ttgaattgtt ctgcctgatt gactaagcca gctgccagaa tatgtggaaa
    16861 atgacttagc attcctgtta tacggtcatg ttctcttgct gtaagagtta catattttgc
    16921 tttggttccg caaaatagtt cttgcagttt ttcaacatct tttttcgtct tttccaaagg
    16981 tgtaaagatg taataggcat tttcaaatag tttagcatct gctgcctgta tacctgattt
    17041 atgagaacca gccattggat gtccaccgac aaaccgaaga tttttctgct cagcggatgt
    17101 cacgacttgc tgcttcgtac tgctgacatc cgtaatgatg acgtgatcag acaagttgat
    17161 tctttcaagg acatcaagat aagctaaggt tgtacggacg ggaacggcta ataagataac
    17221 gtctgcttgt tctgcacctg tttcaaaatc acttgcaacg taatcaacga tctgctgttc
    17281 tactgcttgc tcacgggttt ttgactgact atcccaacca actactatca gttctggatt
    17341 ggtctgacga atacaaagtg ccatagaagc accaatcagt ccaagcccca cgataaaaac
    17401 atgttgtgcc atcttatttg cccccttttt agtaatgttt caagtagtca cggtactcct
    17461 tcactgcagc tttcaactca acaagcgaat cacttgggaa ttttgacaat agctctgtcg
    17521 ctaccacagt ggctacaaca ttttccgcaa cgacgctagc tgcaggaact gctgtactat
    17581 ccgaacgttc tacacttgct ttgtaaggtt cttttgtatc gatatcaaca ctttgaagtg
    17641 gcttgtacaa tgtaggaatg ggtttcatga ccccgcgaat gacaagtggt tcgccattgg
    17701 tcattccgcc ttcgaagccg cctagattat tggtacgtcg gaaatagcct ctttcttctt
    17761 cccataggat ctcatccatc acttctgagc ctggtaatga accagaagtg aatccgaccc
    17821 caaattctac gcctttaaag gcattgatac taaccacagc ttgtgcgatt ttggcatcta
    17881 gcttcgtgtc ccattgtacg tagcttccta atccagctgg aacgcctcca acgagcactt
    17941 ctacaattcc tccgatcgta tctccatttt tctttgtctg atcgatcaaa tcttttattc
    18001 tttgctcaac tgctggatca acaactcgta cttctgacgc ttcagaaatt tctttgattt
    18061 cttgaatcga gatatttcca ggtatttctg cttggattcc accaagacaa atcacgtgtc
    18121 ctgctacgat gatgtcaagt tcttccagaa ttttcttggc aattgcgccg attgctacac
    18181 gcatggtcgt ctctcttgca gaggaccgtt caagcacatt ccgaagatct cgatgatggt
    18241 atttcatacc gcctaccaaa tccgcgtgtc ctggacgcgg acgtgcaact cgccgcagtg
    18301 attggtcttt ttcaggcact tctgctacag acatgacagt tgtccaattt ttccaatctt
    18361 tattttctac cactaaagtg acaggtgaac caattgtttt gccgtgacgg atgccagacg
    18421 tgatccgaac acgatctttt tcgatcttca tccttcctcc tctgccgtaa ccagcttgtc
    18481 tgcgagcgag ttcgtgattg atttcttctt ctgacagagg taatcctgct ggtagtcctt
    18541 caataatggc tgttaattct ggtccatggg attctcccgc agtcatataa cgcatcttac
    18601 agtcctccta agtaatcttt gaatgtctct gtgggaacag gaacgatttt tgcttgccca
    18661 atcttttcta gcaagatgat attcaattgg cttcctcttg ctttcttatc atgggttatc
    18721 gcgtggaata attcatctgg atgacttaga tcgtacgtaa tcggcagatg gaatttttga
    18781 agtatccttt ctaaagcagc tgtcgatcca gttggagaca agccgatttt ttctgcatga
    18841 gaagtgatgg ctaccattcc aatagctact ccttctccat gactgactac cccatatcct
    18901 gctgtatttt caattgcatg gccaattgta tgaccaaaat ttaaaataag tcgatttcct
    18961 tggtcaaaga catcttcttc cactactttt cgtttgattt ccaacgtagc agtaatgatt
    19021 ggaacagcat gttccagaag atctttttcg tctttcagct cgttcagctg tttccaaagc
    19081 gttgcatctg ctatagctgc tgatttgatg atttcagcga ttccttctcg taaacgacgt
    19141 accggcaaag tttttagaac ctcaggatca atcaatacac cagctggctg gctaaatgtt
    19201 ccgacaaggt ttttcgcttt gtttgtattg actgctgtct tcccgccaat actagaatct
    19261 acttgtgcaa gtagcgtagt cggaatttga acgaaatgga tcccacgcat gaacgtcgag
    19321 gccacgaatg ctgctagatc acccaccatt cctccaccaa gtgcaagtat cccatcgctg
    19381 cgggtaaaat cattatctgc taaaaaatcg tataactcag ctgcttgttc taaagattta
    19441 ctggtctcac ccgccggtac gattgaaagg actggttcaa aattagcttg tctcaattgg
    19501 aacatgattt tttctgcata tagtggagca acgtttgtat cagtgatcac agctactttt
    19561 tgaggtctcc aaatcgtttg aagccatgat ccagcctgtt ctagtgcgcc tttttcaata
    19621 tgaatatcgt atttttggtt atcaacatgt acagtaagca ttcgtttcac ttctttcttt
    19681 taaaaagctg gaattaatcg agtgtctgaa tcttttccat tgcttctttc atgatcgcaa
    19741 cttctttcat catgcgtgag taagtctttt cattcaacga ttggggaccg tctgaccaag
    19801 catttgcagg atcaggatgg atctctacga tcatgccatc tgctccgctt gccacacccg
    19861 cacgagccat tggtggtaca agttcccaaa ttcctgtacc atgacttgga tcaacaatca
    19921 cagggaaatg agttaatttt ttcatcaatg ggacagcact taaatcaaac gtattacgtg
    19981 tcgctgtttc gtaagtccga atcccacgtt cgataaaaat gacaggagat ttgtcttcga
    20041 ctgcaatgta ttctgcagca ttaagccatt cattgatcgt accggaaatt ccacgtttca
    20101 atccaacagg tttacctgtt ttaccgacag cagatagcag tttaaaattt tgcatattac
    20161 gtgcgccaat ctgtaggata tcgctgtact cagccaccat atcgatatgc ccttcatcca
    20221 tcacttcagt aatcactttc attccaaatt catcagccgc ttgacggatg tatttcaatc
    20281 cttcttcttc taaaccttgg aaagcatatg gagaagtacg tggtttgaag gcaccacctc
    20341 gcagaatttt tgcgcccccg gctttggcca ttcgcgcaca ttctctgatt tggtctaatc
    20401 cttcgataga acaaggacca gccatcgtca cgaaactgcc gtcaccaatt tttactccgt
    20461 ctacatccac gactgtattg ccaggatgga attctcgaga agtcaatttg tatgtgttta
    20521 aaatacgcac agcattttct actccttcat agcgtgtaaa tgctccttct tgtaaacttc
    20581 gcgtatcacc ttttagtcca atcactactc gttctgttcc ttgatcgatt gagactttga
    20641 atccttcttt ttcgatatga tcgacgactt tttttatttg ttcttccgtt gcttcttttt
    20701 tcataattac tatcattgtt tttcccgctt tcttttgttt acttatctat cttggctgat
    20761 tttctctact ttttcaaggt ggttttacat taattcctac taaatgaccg tgtcctgttg
    20821 ttctcatttt cgctccttct ttcctactta tgacccagat aatcaacaaa aaaagatgtc
    20881 cctgtaggaa attgcctcta cagtgaacat cttcaacgag ccttaaaaag cgctgcaaaa
    20941 atataaaata gccactatag atgaatttct ctacgtggct gtaagtcgtg tttaaaaatc
    21001 tttagccatc atagatacaa acctggtttg tttgtaccca cgaagtcatg aatacaaacc
    21061 tatctaaaat agctaaagta atacgtattc agttttgtaa gttttctaga tttcataaaa
    21121 aatcgctcca aactgatgta agaattgtac ctagtataga cataaaaaaa tcgactgtca
    21181 atcattttta tgaaactttt tttaattttc tgatagttaa ctttgttttt cagaaaaata
    21241 acaattggtt ttattgaaga aaatagtcat gaacaaaagt atctgagaac ttttttactc
    21301 tcttgttcat gacatataga ataaagcagc tttgtcttgt aaactccgtt tttcaaataa
    21361 taatctaaag attgctactg aataataaag ccgatttcat ggtcctcttc gctgctgatt
    21421 cggtaaggtg actcaacatc cgctccccag ctattgatac ccccgactcc tcttactttt
    21481 gcaaaaatcg taagaaccgt tcgtctttct ggcggtagtt cttctatatg tgttgcctgt
    21541 tccagttctt ctgctttgaa tggtaaagcg gaaaaagcaa atggcgtatc ctgctgttca
    21601 aatcgcaacg aaaaatcttc ttgatcttta tccgtattat ccaacgtttg atttcttgtt
    21661 agttctaccc atttcgtttc catatgcatg ccgcattctt ggggaacaag gtatggggaa
    21721 acggggagtc ctgccacttt ataaatacct tcgacaccac cagccatccg atccggatat
    21781 gtttcccctg ataggccttc atactcaaaa aattttgctt tcgttggcat aatgaacctt
    21841 aaacccaatg cgggtaattc cggtaatcct ttttgtccat aataatgcat attgacaagc
    21901 attttaccct ctccagcgat ttggtaggaa attgtcactt gggtttttgg tactgtgttg
    21961 atttccagtg taaaaatgat ttcaaccgtt tcagcgtatt cattacttga atattgatta
    22021 tttactggag caatcggcaa atcgatcttt ttattgtcca ccagtacttc tatttctttg
    22081 acttttggat acaatccggc tcctaaccac ataccggatt taaatccaaa tccgctacct
    22141 ctgtcattat cggtcgttgc tcgccaaaaa actggaagtg tttcccggta tagccattct
    22201 tttccgtttt tattgagcga ttccagtccg cctcttgtat aagaaaaaat ataatgcgta
    22261 ttccccgatt tgacacctaa tgtacagtcg ccgaagacga ttgcaacttt ttccgtatcc
    22321 atatgacttt tatttactgt atttttcata ataatatctt acctcctgca tggcgggttt
    22381 ttcttgtcca tctgcaaaaa cgatcccatt ccctgagaat tcatagtcac ttggtttatc
    22441 gtcaaagtca ccgccatagc gcatcacttc tctgcctgtg attggatcag tcacttggat
    22501 cgcctgatct ttaaaatccc atatatagcc tccttggtac atttcataac gatcaagcaa
    22561 cgtcatgtaa gaatccattc cacctaacga attccccatg tcatgcatat attcacataa
    22621 gatgaacggt ttttccgctt tattttcaag gtagtcaatg atctcttctg gtggcgcata
    22681 catgcagctt tttacatctg aaatcgaatc ttcgaatgct ggatttggaa aattcccttc
    22741 ataatggaca agtcgatcgg ggtctacttt tttgaagtaa tcgttcattt gtcgaatgtt
    22801 ttcttccgca taagattcat ttccaagaga ccaaaaaaga atacttggat ggttttttaa
    22861 tgtttcgaaa ttcgtttttg ctcgatcaag aacagccgcc tgccactgag gtactgcacc
    22921 tggtacgttg taagaaggct caaatgcgcc cattttttgc catgagccat gggattcgag
    22981 gttcgtttcc gccatcacat ggattcctgc ttggtcgcat aaataatacc aagtgatctg
    23041 gttggggtaa tgagatgtcc gtacgccgtt gatctttgca ttttttatga tagccaaatc
    23101 tttttccata tcttctatag tgatggcgcg tccttttttc ggatgccact catgtcgatt
    23161 taccccgttg ataatcatcc gttttccatt gaagtagatg attttatctt tgatttcgat
    23221 tttacgaaaa ccgaaggaat agtcaatata ctccgttact cttttttctt cgtctatcgt
    23281 ctccaattca agcttatata aattgggctg aaaatgcccc cacagtgcca cattttcaaa
    23341 tccatcgatg cgaatcgtca tttgctcttt taacggatat tgctgttgat agatttcctt
    23401 cccatcagga tctagtatcg ctacattcag ctgactgttt ttggctactt gctcaaaaga
    23461 caaatctact tcaagtttgc cattttgaca tttgtcatct aagcgaggtt tgatccaaag
    23521 atcttctaaa tggttttgtc ctcgagcaac taagaagaca tctctgaaga tcccaaaaaa
    23581 tcggaaaaag tcctgatctt ccaagaatgc agctgtactt cttttgtaaa cctccacagc
    23641 tagaatattt tcttcttcca aataaggtgt taaatcaaat tcagacggag tgaacgaatc
    23701 ttcggcatac ccgataaaat gaccattcaa ccaaataaac attgcttgtt ctacaccagc
    23761 aaaatgaatc gagatttgtt ttccctttag accttcttct aatcgaaaag tcgtcaaata
    23821 cgaaccaacc ggattgtctt tagcagctga aaagatccct tccgcctctt tctcagggct
    23881 tgtcgtgaaa gggggacggc gaaatacatg tccctcccac ggatacatcg tattgatgta
    23941 atggatcttg tcataatttg ctaattcaat atgactagga acagtgattt catcaaaaga
    24001 acaggcatca aaatcttttt tataaaatgt ttttggtcgt accgaaggat ttggcgaata
    24061 acaaaaacgc catgatccat ttaaagactg tttaagggag gatgtggttt ctacttcttc
    24121 ccttgttgca taacaaacat gatcgctatg tgcatttgat tggttcactc taaaaacttg
    24181 tggatcatct aaccatttta tatttgcttt catacttttt tccttctctc tcataacttt
    24241 ttgaaatgaa gcagttctgt ttggaaatca ccttgaaaat cggggaagta aagtccaact
    24301 gtcatcaatt cggcccccga aaatatccgc ttcgaggtct gttcctgata aagtgcctca
    24361 tcatctaacc ccatcagata tacttcatgg aacgctggtt gagctgaagc tagtatcctc
    24421 cctaaaaaca caatagcttc atcttgttta ggagacacaa acatccatgc ggcaatattt
    24481 tcttcaaaag gactcgccaa acgataataa tggccatact ggatcaccgg acgaatcgtt
    24541 tgataggata taatttggtc ttttacttgc ttttcttctt caggagacag ctttgtcaaa
    24601 tccagttcat aaccaaaaac agcgctcata gctgtttctc ctcttgtttt caacgaagta
    24661 gatctgtcgg tctgatgatt tggtacagct gatacatgag ctgtgatgga cgaaatcgga
    24721 taagcaaggc ttgttccata ttggattttc atacgctcca ctgcatccgt gttatcactt
    24781 gtccatgact gcggcatata ataaataaac ccggcatcaa accgtccacc tccaccagaa
    24841 cagccttccc ataagatttc cggataatca gttgtcaaat gttcaagtag ttcataaagc
    24901 ccgagcatat aacgatgcgc tacttctcct tgtctctgtg ggtcaagggt aatcgaatag
    24961 acatctgaaa gcgaacggtt catatcccat ttgatgtagt caagaggaat cgtatctaaa
    25021 atctttctca tttgtttctc aatcgtctgc cgaacatcta accttgtaaa atccagtaca
    25081 tgttgtgatc tggaagcaga aggcattcta cctggctcct gcataagaaa atcaggatgt
    25141 gttcgataca gttcagaatc aaccgagatc atttcaggtt caaaccataa accaaatttc
    25201 aatcctttgc tatgagcata atctgctatt tcacgtaatc catttgtcaa cttgccttca
    25261 tactcgaacc agtctcctaa tgaagaattg tccgcatctc tttttccaaa ccagccatca
    25321 tctaatacga acatttcgat gcctagctcc tttgcttcat caatgatcgc cttgattttt
    25381 tcactattga agtcaaagta agtggcttcc cagttattga ccagaatcgg ccgttcagca
    25441 aattgatgac gacctcttgc gattctttca cgtagcaact catggtggat ctgactcatt
    25501 ttgttcaatc cctgacttga gtaagaaagg atcatttcag gtgtttgaaa agactggcct
    25561 gcattcaatt cccacgaaaa acgatggctg ttgatcccac cgacgactct caattgattg
    25621 atctgatctt tttcaagttc gaaagaatgg ttgccggaat aaacaaattg aagtccgatc
    25681 gcttctccag tattttctgt cgtatgatga tgaactaaag caataaagtt attcatatgg
    25741 tgactgctcg ttcctcgacg gctttcaaat acttttgttc cagataaaac agattgccgt
    25801 gaaatctgtc tttcattgac atgtgctcct ggcaaagcaa tgacttcatc aaatcgccta
    25861 gtatgggcaa agtctagctg gaaactagcc gctttttcta gtttgattgt ttctccggtt
    25921 tcatttctta tctttacaga tcgtgtgatc actgctctat cagtaaatat ggtatagaac
    25981 aaatcgaaat aaatcgctgt ttctctatcc ttaagtgaaa tcaccaacgt ttgtgcctct
    26041 gacttgtcca tcacataagt ttgaggcaag ccttcgatct ctggcttccc atctgtaata
    26101 taatggctgt catatctcag atccagtaag tctgatccat tcttgcgctg aatgatcgat
    26161 gcgggcgttc gaaagtccat gctgccatga gaagagtatt cttgcggtag tgtatctttc
    26221 gacagagacc ggtcaggatt taagggaaca tttcctgaaa atccacgatc tcttcgggga
    26281 taatttttat tgtctttgta ctgcttgact ggtttgccaa aataaatatg cgccaataca
    26341 ttgctttctt ctatctttat cagataagaa agaaatgtat ttcgtaaatg gaagactcgg
    26401 ctcttttcat caaataagat gaaattttta tctgtttttc tatgcgtaat atccatcatt
    26461 tcactcccaa caaaacggaa accgtttact tttttttata attcctgtat actgtttact
    26521 aaacaatttt gtcaataaaa attttttatt tttttgagga gtgtgtaaaa atggttggaa
    26581 ttagagatgt cgcacgaaag gcaaatgtct ctcctgctac agtttcccgt gtgttgaata
    26641 acgatcagac gatccatgtg gctcagacta ctcggcaacg gatagtagag gcagctgctg
    26701 cgttggatta tgacatcagt actcgtaaat atatcaaaaa gagaatgcct tcgattggtg
    26761 tgatctctac gatcggcaaa gcgagcgaag aacaggatat ctattacaaa catttgcgag
    26821 tagggatgga agaagaagct cgacgtctgc atttagggat gaatcgaatc tataatctat
    26881 ctgataatcc taaacaatgg gaagatctag atcagttagg tgcggtgatc gttgttggga
    26941 ctgttacgaa agaatcgatt accgccttgt taaaacaaaa catgcaaata gtcgtcgtgg
    27001 ataaccctga catccagcaa gaggtcgata tggtgtatgc agatcttgaa cggatgacta
    27061 aaaccgtact ggaattgttc ttagaaaatg gtcataaaag aatagcctat atcgggggat
    27121 ataaagtcga tatggatgaa ttcggtggga agcagatttc tataaatgaa aagaggatgc
    27181 gtgcctatca gcattttatg atcgatcatc acttagaaaa atatatgtct tatcatctag
    27241 gtggctggac agaaaaacaa ggtgaggtac ttgcgactaa tctattagaa aatgaatccc
    27301 ctcttcctac tgccattctt gtcgggagcg atccaatggc aattggtgtc tatcgggcat
    27361 tgaaacaaaa aaatatccaa attggagaag agattgttgt agcaagcttt gataatatcg
    27421 agacaatcgc aaacctctcc cctggcttga ccagtgttca aatcaatgcg aaatcaattg
    27481 ggaaagcagc tgttcgaatg gcattggagc gaattgacgg tatacgggac gaaccaatca
    27541 ttttgactta tcctgcacga ttgattgtta gggaaagttt tatcccaaga acaaaaaggt
    27601 attgaaagag aggttgtgaa aagccgcttc gtttcttcat actcaaaacg aaacggcttt
    27661 ttcacaacct cttacttata cttattcaca aaatccaatg tctgctggta acaattttgc
    27721 gattctttta gcgtgtaatc aaattggtat tcatgggtaa tctctttttt tgtatctgta
    27781 taaaacaagg attgcacggg tacctttaat tctgtcaacc gattttctaa tgcttgacct
    27841 tgctcctgga atgagtatgc atttccgtca gttatatacg ttggcggaaa ttccttagaa
    27901 acatggtcag ccacactagc ttctttcaat tgctcactcg ttttccaatc ttttgttcca
    27961 atcaacgacc acgcaacggt tttgacgaaa aacttcataa atcgatcatc cgaatgctgc
    28021 tgtgctgtta gcttcaaatc aacaggtccg caataagaaa tggttccttt cagactatct
    28081 tttggaagta gatgtttgat tttcatttgt ttagcatacg catcattcgt ttgtatagct
    28141 gcatattgca atgcgatttg tgaacctgca ctatctccac caaaaaataa ttgttccatg
    28201 tctagcaatt tgtcgtcttt ttcttcagct aataaatctt tgatcagttc attcacttga
    28261 agaacttgat tagggtattc tgaatcaggc gctaattcat aattcatcgc aaccactgcg
    28321 atgttggcgt ctgaaactaa ttttgtcgca aattctttga cacccgattt atctccgccg
    28381 acgaatcctc caccatgtac ccaaaataga acaggaacag gccccttgct gttttttgga
    28441 tagtagatgt cataagtatt ctctttatac ttggactcat acgtcaaatc tgtttttttc
    28501 gccacgtttt tttctgcttg atcgaacgtt tttttatcag tgatttgaac agtatttgaa
    28561 aacatatggt tgatcaagaa tgctccaggt ctgggggaaa gctggaaggc aagaaagatt
    28621 gcaaacgcga tcccaagaat gattccgaag aaccatccga taaatttaag aattttcttt
    28681 ttcataaggc tccctcagta gttagtatta agcattctga ttataccata gtgttatttc
    28741 ctatcaagct ctcctagatg atttaatgat tttccccaaa cttcttctgc aatgcttttg
    28801 acaaaagaaa ttttttccat tgttcttcaa acaaaacatt ttcttcttct gttgaagcaa
    28861 atccacattg aggacttaag cacaaacgat ccaaaggcag atatcgtgtc gcttcgtaga
    28921 tccggtcaat cacttgctgg tgctcttcca tttcgccatt tttcgttaca gtcaaaatct
    28981 aagacaacta atttatcttc agagactttc tctaaaagaa caaaactccc agcacgttca
    29041 ctatcgtatt ctaaataata ggcacggaca ttttcttgag taaaataatg ggtctgctac
    29101 gtcgtcatcc cccctgatgc cgccaaaatc gaataataat ttccgcgtca aacatgtata
    29161 tttagtgttg aatcatttga gaatatgaaa ataaaaatct ttttgatcaa tcagttaatc
    29221 aaattgaaat aacgaaaaat ctgagacaga atgacgttca ttctgaccca gatttgattt
    29281 tgcacaactc aacagtcctt attggcaaat cgccaaaata atgtagacta actaaaaaga
    29341 ctaagaaaca ataacaaaag ttaacacttc ttagtctttc tttagcacgt ctatttatta
    29401 aagattttag ggccttttct catgttttta cgatcattct taggtgtttc catttgtttg
    29461 gatcggcctc caccttgttt ctttttgatc aattctttca ttttttcttt ttggtccatt
    29521 tttattctcc tttaacgaat gaagcgacta attcgatatg atgggtttgc gggaaaagat
    29581 ctacaggctg aactttcttc acggcataac ctgcttctgc aaataactta gcatctcttg
    29641 caaaagttgc cggattacat gagatataga cgattcgttc tggtgacatt tcgattgctg
    29701 attcaataaa atgatgatct aatccttttc gtggagggtc aactacgatc acagatggtt
    29761 tgatcccttt ttcttgccat tttttcataa ctttttctgc ttttccgact tcataatgaa
    29821 cattttcgat tttattattt aaagcattga actgtgcatc ttcaattgct tctggaataa
    29881 cttccatccc atacacctta tccactctgt ctgctaaaga caagccaatc gtacctattc
    29941 ctgaataagc gtcgatcaca atgtcttttt tatctaactc ggcaaactcg atggctgttt
    30001 ggtacagata ctctgcttgt tgagtattta cttgataaaa tgactttgct gagattcgat
    30061 aggtttttcc taatagctga tcttcgatat agtctctgcc atataaaact ttttctttgt
    30121 ctcctaaaat cacatttgtt ttttcttcat tgatattttg gatgaccgaa accacttctg
    30181 gcaattcttc atgaatagct tcagcaattt ccttggattt aaagaatttt tctttacgag
    30241 taactaaaac gaccatcatt tcatgggaat aatgaccacg acggataatg atgtgacgaa
    30301 gctgtccttc atttttttct tcattatatc ctcgtacttg gaaccgttgc aagatatctc
    30361 tgactacttt gattgcttga tcgatgacag gatcttggat ataaaaatct tcgataggga
    30421 ctagatcatg gctattcttt ttgtaaaaac cggtggctaa ctgtccatcg atacggcgaa
    30481 cagggatctg tgctttatta cggtagccga atggttctgc cattccgacc gttggaagta
    30541 caggaacttc tggcatctta gcaatcttcg ccattacatg ctctacttgt ttttgtttga
    30601 agacaagctg ttgttcatac ttcaaatggg ctaaaggtgc gatacctatg cgaagtaatg
    30661 cattgttttc tacaggctga cgatcaggtg attgtgtaat aaatgtttcg acttttccat
    30721 atccaaactt attgcctgct tttactacta gtatttctac ttcttctcca gggagtgcat
    30781 tttcgataaa taatggataa ccatcgattt ttgctacccc cattccttca taactcaagt
    30841 cgacgatttc gactgtatat cgttcgtttt tctttactat ttgctgttct gtcatatttt
    30901 gctcctttaa gcctattttt caatcctact gaactctact ataaatcagc taattacaca
    30961 actaaaaccg gcactgacct tactacgttc agcaccggct tttgattaat tcttttgatt
    31021 aggtgtttcg tctgctattt ttccgtcacc gtcgatatct tcatccgtta aacgttcgac
    31081 ttcttttacg aattctttgc tgacaacctc taattcttct tcatctgtac ctgtgattgc
    31141 ttcatcagga atctgatcgg tattggcaaa gaactcaatg tgttgatgaa gattttggaa
    31201 atgcatcggt gcttccccgc cgtattcacc atctaagttg atcattactg gtcgattgat
    31261 atcatcttct gtttcaacga acaaactgct tgttttggta tagattattc ttggatcatc
    31321 gacatgtcga ccgccattca gcatgagtgc tgcaatatgc aagatctcaa agatatttgc
    31381 tgtcttcaca atgattaaag aaaacttccc atcatccaat ttggcatctg gcgcgatctg
    31441 ttcgaatcct ccaacagagt tcgtcagccc taggaaaaac atcgaagcat ttccgatata
    31501 ttccccttca tcatacttca agcgcatttt gattggcttt actcgaggga gcatctctgc
    31561 tcctttcgca agataagcaa gataaccaaa aatactcttt agttctgatg gaacttcata
    31621 agtcaattct gtcaaatagc cacctgcagc gatattgatg aagtaactgt tatcagaaag
    31681 gccgatgtcc atttttacgg tttggtgttt ttttatcact tctgctgctg ctttaatatt
    31741 atctctagga atttttaatg ctcgagcata gtcgttcgtt gtaccagctg ggatgatggc
    31801 cattttagga cggtgttcaa gcggagcgat tccattaacc acttcgttga ttgtgccatc
    31861 gccaccagct gccactacta gatcaaaacc tagttctgcg actctacatg cttcattctt
    31921 ggctgaatct ggttctggtg tagttgcata ggcgctggct tcaaatcctg cttcttctaa
    31981 tgcctgtagg atatccgcca aatttttttt caataattct tttcccgagg tggggttata
    32041 aattacgcgt gcttttttca ttttcagcct ctatcgtttc gctaattcat ctttaagtag
    32101 cttattcacg acaccaggat ttgcttgtcc tttagttgct ttcatgattt gaccgactag
    32161 gaagccaacc gcacgatctt ttccattttt gaaatcatcg acagattgtt ggttgttatc
    32221 caaaatttca ttaatgattg gcagtagttt gttcggatca gataattgca cccaaccatt
    32281 tttttcaact acttcttggg cttctccgcc atttgtgatc aattcacgga agactttttt
    32341 ggcgattttt gagctgatcg ttccatcggc gatcaaacga atcattcctg ctaagttctc
    32401 aggtgtcaat tttgtctcag ctaattccag tttctcactg ttcaagtaag cagaaacttc
    32461 acccatcaac cagttagatg cttgcttggc atctgcacca ttttccaaaa cagcttcaaa
    32521 aaaatcagac atttcttttg taagtgtcaa taccattgcg tcatactctg gaagtccaaa
    32581 ctcacggata tagcgttcgc gacgagaagc tggcatctca ggcaaactag cttttacttt
    32641 ttgaatccat tcgtcatcga tcacgaattt cgggatatca ggctctggga agtaacgata
    32701 atcactagac ccttctttga cacgcatcaa aactgtttta ttcgttgttt cgtcaaaacg
    32761 gcgtgtttct tgttggattt ctcctcctga aagaagaact tttgcttgac gtttttcttc
    32821 aaaagctaaa ccttttttca cgaaagataa agagttcaag tttttcaatt ctgctttcgt
    32881 accgaactct tcttgtccat aagggcgaag agaaatattg gcatcacagc gcatagagcc
    32941 ttcttccatt ttcacatcgc tgacttctgt aaattggata attgatcgca atgcttctag
    33001 ataagcgtat gcttcttctg gtgaacgcat atcagcttca gaaacgattt cgatcaaagg
    33061 cgtgccttgt cggttcaaat caacgtaaga ataaccgcca ataccatgca tgttttttcc
    33121 agcatcttct tctaaatgga cacgttcgat ccggattttt ttctttttac cttctacttc
    33181 gatttcgatc catccgtcat gaccgattgg ttgatcaaat tgggaaattt gatatgcttt
    33241 tggattatct gggtaaaagt aatttttccg gtcaaaatga gtatctttag agatttcaca
    33301 gtttaatgcc aaagctgctt tcatcccgaa ttccagcgca gctttattca ttactggcag
    33361 cacgcctgga taaccccagt cgatcacgtt tgtattgctg tttggttctg caccaaaatg
    33421 agctggcgct ggagagaaaa tttttgagtt ggtttttaac tcgacgtgga cttctagtcc
    33481 aatgactgtt tcaaaattca ttcgtctttc cctcctaaaa tagctggttg ttgtttatgg
    33541 aattctgttg cttgttcaaa cgcatatgcc gcttgataca tcgttttttc atcaaaataa
    33601 ttaccgatga tctgcaatcc aactggtaat ccttctgata aacctgccgg tacagacata
    33661 cctggaagtc cagccaagtt gacagggatc gttaagatgt cactcatgta cattgtgatt
    33721 ggatcgttaa tgttctcacc gattccgaat gcaactgttg gtgaagatgg cccgatgatc
    33781 aagtcataat ctgcaaagac tttttcaaaa tctcgtttga tcaatgtacg gacttgtcct
    33841 gcttttttga agtaagcgtc ataataaccc gcgcttaagg agaatgtacc taacatgata
    33901 cggcgtttta cttcttcacc gaatccttct gaacgtgagt tgacatatac atcatctaat
    33961 gatttcacat tttcagagcg ataaccgtag cggataccat cgaatcgttg caaattggaa
    34021 cttgcttctg atgaggcaat gatataataa acagccacac cgtattttga atgaggcaag
    34081 ctgacttctt ccacaacagc acctaatgaa cggaatgttt ctgctgcttt caatacagct
    34141 tcttttacgc caggggccac accttcgcct aagtattctt taggtaaagc aatcttcatt
    34201 cctttgatgt cgcctgtcag tccctctgca aaatcaggaa ctgaaacgcc tgaagaagta
    34261 ccgtcttttt cgtcataacc gctgatagct gtcaatgcta atgcattatc tttcaccgta
    34321 cgggtcattg gtccgatttg gtcaagagat gaagcaaagg caatcaaacc aaaacgtgag
    34381 acacggccat aagtaggttt catccctacg atgccgttaa aagctgctgg ttggcggata
    34441 cttcccccag tatcactacc taaagaaact ggaatttgtc cagctgcaac tgctgctgca
    34501 gaaccaccag aagagccacc aggtactttt gttgggtccc aagcattttt tgttttcttg
    34561 aaataagaag tttctgtact tccacccata gcaaattcat ccatgtttag cttaccaaca
    34621 gggatcatat ctgcttgata gattttatcc atgaccgttg catcataaat tggttcaaaa
    34681 ttgtaaagga tcttagatgc agcggttgtc aagatatctt ttgttacgat attatccttg
    34741 ataccaattg gaataccagc aaggacgttg ctttcatcaa tccctttttc atctaaagct
    34801 tttgcctgtt ccaatgcttt ttcttcgttc aatgtaatga atgcctcgac ctcaggctct
    34861 gtttctttga ttcgttcaaa tgttgttttt gtcaaatctt gtacagtgat ttctttcgat
    34921 acaagcatat gatgcagttc ttctagagaa cgattgtgta attctgtcat tatgcgccag
    34981 cctccccatt atcgatgatt gcgggtactt tgataaagcc gtcttctgtt tccggcacat
    35041 ttttcaataa ttcatcgcgg ttccatccag cttgcgcccg atcttgtcgc atcacgttta
    35101 ctgtttcaac cacattcgac gtaaatggaa caccttccgt atctacttct tccagcattt
    35161 caaccatatc gatgatttta cctaattggt cggtaaaact tgcaagttct tcatctgcga
    35221 acgcaagttt tgctaatttg gcaacgtgtt tcacttgttc ttcactaatc gccatactac
    35281 cccttctttc taaacgagtt aaaaactttg ctctctattt taacacaaga gtgccgtgtc
    35341 tgaaattgtt tattgaaatt tgtctgaaaa aactcatttt tcattcttct actgagcgac
    35401 ttttagccaa tgcatctgcc atttgatctt ctgtccaaac ttcgattcct aattcttgtg
    35461 ctttggtcag cttgctgcca gcatcttctc ctgctaccac gatatccgtt tttttcgata
    35521 cgctaccagt cactttcccg ccaagatttt cgatcgtctc ttttgcttct tcacgagtaa
    35581 atcgtgttag tttaccagta aggactactg ttttttcttt aaaaggagac tcgacttctt
    35641 gcaattgggc atttcgaagc cctttgtatt cgaaattgac gcctgcttgt tttaattcat
    35701 tcatcaattc atggacttct tcgttactga aataagtgac tacactgtcg gcaatgatat
    35761 caccgatcgt atcaagcgca ataatcgact catagtcact tttactcaat gtttctagat
    35821 caccgaaatg ttctgctaaa atttttgctg ctttcgctcc gacatgtcgg attcctaacc
    35881 cgaaaatcaa ccgctcgacc gaattatctt tgctgttgtc gattgctgtc aaaatattat
    35941 ttgctgattt ttcttttatt ttgtccaacg tcaataactc ttcttctgtc aatttgtaaa
    36001 gatcagcaac atctgctact agttttttat catacatctg ttctagtacg cgcggcccca
    36061 acccatcgat attcatcgca tttcttgaaa caaagtggtt caaaccttct ttcatttgag
    36121 cagggcattt aggattgatg caccgtaagg ctacttcttc atccaaatgg acaagttcac
    36181 ttccacaaac tggacaatgt gtaggcaatt ggtattcttc gctgtcttta ggtcgtttat
    36241 ctaacacgac ttgcgaaact tctgggatga tgtctcccgc tttataaatc agaactgtat
    36301 ctttcaagcg gatatccttc aacttaatat aatcaccgtt atgcaggctg gcacggctta
    36361 ctgtcgttcc tgctactcgg actggtgtca tgatagctgt tggcgttact acccctgttc
    36421 gaccgactgt ccattcgatg ttttcgatta gcgtttcaac ttcttctggc ggaaacttgt
    36481 aagctgctgc ccatctcggt gctttgatag taaaaccaag ttgatcttgt agcgagaatt
    36541 cattcacttt gatgacgatt ccatcgattt catagggcaa atccacacgt ttgtcatgat
    36601 attcttcaat gtatgaccac acttcatcaa tcgaatgaca tagacgtttt tcccgattcg
    36661 tgtggaatcc aattttttct agctcttcta gcgcatcata ctgggttttt gcttgcatag
    36721 gaccgaaatc agctactgtg tataaaaaag tgtccagatt tcttttcgct gtgattttag
    36781 aatctaactg acgcaaagaa ccagctgcgg cgttccgagg attcgcaaag atatcttttc
    36841 cctctgcttc tctgtcttgg ttcaattgga caaatgaccg ttttggcatg aaacattcgc
    36901 ctcttacttc gatgttcatt ggttctttta atttgattgg gaccgaccgg acagttttta
    36961 ggttttcagt gatgttttct cctacagttc catccccgcg agtcgcacct ctcacgaaat
    37021 ttccatcttc ataacgcaaa gaaacggaaa gtccatctat ttttagttca cagcaatagt
    37081 caactacacg tcccactgct ttttgtacac gttgatcaaa agctatcagc tcttctttgc
    37141 tgaaaacgtc gttcaaactg taaagcggaa tatcatgagt gactttttca aaaccttcta
    37201 atacttttcc gcctactcgt tgagtgggag aatcagacgt gatcaaatca ggatattgtt
    37261 cctcgattgc tactaattct gcgtattctt tatcataaac atagtcttcc actgttggtt
    37321 tatcttctac gtagtattcg cgagaccatt ggttcagtct tgtacgaagt tcttctgctc
    37381 gtttagcagc ttcgtcaatc gtcatttcat gttccatcat ttaccctcca ctactaagct
    37441 ttttcaattg gcgcaaaggc agccaaaaga cgttttactc cttgacttgg gaaagccaca
    37501 tctagttcta gatccttcga cgtcccgctt actcggacga ctgttcctag accccatttt
    37561 ttgtgtttga ctttatctcc aggtttccag ctgttatttt cccctccagt agcactttta
    37621 gagggaacaa ccgctgtctc tggctgttta tagctatatg ctgttttttt agcgaataca
    37681 ggcgtttttt taggcgtagg acgcatgcct tcgatttcca gtaactcctg atcgatttct
    37741 tctacaaaac gagaaggtcg gttatattgc gtccgtccat ataatgttct ggagaaagca
    37801 tttgttaaat acaacgcttc ttctgctcgc gtgatcccca catatgcgag acggcgttct
    37861 tcctccagtt cgctttcttc catcaatgct cgagataaag gaaaaacccc ctcttctaga
    37921 ccaatcagga atacaactgg aaattctaat cctttggctg catgcaaagt catcaatgtc
    37981 acttgggaag catcttcttc taaattgtca atgtcggaaa caagtgccag atcatttaag
    38041 aagaccgtta gtttttcttc tggtgcatca gcgtcttctt cattttgctg ttcgaattgt
    38101 ttgtcgaatt cttgtgtgac cgttaaaaat tcctccaagt tttcgatgcg tgcttgtgct
    38161 tctaatgtat tttggatttt taagtcttct agatatccgc tacgatccaa aacctctttc
    38221 gtcaattctg tcactgtcaa gtatgggatc atctgagtga cttcttggat catctctcca
    38281 aatgcaccta attgctgtcc tgcttttcca ccgatatttg ccagatccac attttgcgct
    38341 gcttcaagca gtggccattc atgaagagaa gcaaaactgc gtagtttttc gatagatcct
    38401 ggtccgatgc ctcgtttggg cgaattaacg atacgctcaa aactgattga atcttgagga
    38461 tttaccagca cattcaaata cgctaaaata tctttgattt ctttacgatc gtagaacttg
    38521 tgccctccaa ccattttata aggaatattt gctttcaaca gagtttcttc cattacacga
    38581 gattgtgcgt tcgtccggta caagatggca aagtcgccgt aattccgatg attgcttcga
    38641 atctcttctt gcatccggtc tacgataaaa cgggtttcgt ctcgttcatt atctgcacgg
    38701 tagtaagtga tcttgtttcc ttctttgttt tctgtccaca agttttttgg ttttcgattg
    38761 ctattgtttt caatgacttg gtttgctgcg ctaaggatgt ttttcgtgga gcgatagttt
    38821 tgttctagca aaattactgc agcatctgga taatcttttt caaaatccaa gatgttctgc
    38881 atatctgcac cgcgccagcc gtaaatactt tgatctgcat ctcccacgac gcacaggttc
    38941 ctaaatctac ctgccaataa attgactagc gtatactgtg cgtgatttgt atcttggtac
    39001 tcatctacat gaatataatg gaatttgttt tgataatacg tgagtgaatc aggatgttct
    39061 tcaaataatc gaatcgtatt catgatcagg tcgtcaaagt ccatacattg attatattga
    39121 agctcttttt ggtaagcagc ataacatttt gccgcaatct cttcgtaata actgccttgc
    39181 atttttgcaa aatcctgagg ggtctgcagg ctgttttttg cttgagagat tgttcctaat
    39241 atacttcgag gatcatattt tttcggatca atgttcagtt cttttagtat ccgtttcatc
    39301 aaggtcagct gttcagaaga atcgatgatt gtaaagttcc ggttataccc gataaaatca
    39361 acatctcttc ttaaaatacg cacacacatc gagtgaaaag tcgataccca gacatcttcg
    39421 cctccagatg cgagaatcgc attgacacgt tctttcattt cttttgcagc cttattggta
    39481 aaagtaatcg ctaggatgtt ccaaggattg acttcttttt cctcaatcaa ataggcaatg
    39541 cggtgtgtca gcactctcgt ctttccactt cctgcacctg ccattagcag taatggtccg
    39601 tcagtgtgaa gaacagcttc tttctgacgg gggttcatcc cgtttaataa ttcagctttg
    39661 gatgacatta acaacatcct ttcttttatc aatcttgtta attatagcat tttcttggct
    39721 ttcattaaac tagctagttc aaaatcattt gtttcttttt atggattttt ccttgtcaat
    39781 tcgttgattt cgcgctaaat taaaagaaag cgaggcgttt gaatggaaaa aaaaggaaaa
    39841 atcaagaaat tagataagac tcagatatat tttattctaa aaggaatatt tgttggactg
    39901 ctgacaggaa tcgtcgttag cctgtttcga ttatctatcg aaaagctaag cgatttagtt
    39961 cgaagtatct atgaaatgag tcgggaacag ccgatttact taattggcct agctatttgc
    40021 tgcatccttg cagcattttt cgtgggttat ctggtcaaaa atgaaccgga tatcaaggga
    40081 agcgggattc cacaagtagt gggacagctt cgcggggaat tatcaatgaa ctggttctct
    40141 gttttatgga aaaagttcat tggcggtgta ctttcagttg gtgcgggcct ttttctaggt
    40201 agagaaggac cttctataca gcttggcgca tccattggac aaggagctgg acaattgttt
    40261 cggagtccat cttcagaaga aaagatttta atttctagcg gtgcaagtag cggacttgca
    40321 gcagcattca atgctcctat cgcaggatta ctctttgttt tagaagaaat ccatcactcc
    40381 ttttctccac ttgtttggct gacatcattt gcttcagcaa tcactgctaa cttcgtctct
    40441 ctttactttt tcggcttgag acctgtttta tatctaggtg accttccttc tttgccgttg
    40501 aaatattatg gttccttagt gatcttagga gtacttctag gcatcttagg ttttatttat
    40561 cagaatgtct tgctggcttt gccaaaattt tacaaccgat tgcctttacc agcttatttt
    40621 cacgggttcg ttcctttctt gctgattttg ccaattggtt atttatggcc tagtactcta
    40681 ggcggaggca atcaaatcgt tttagctttt ggacatgctt ccttgccaat cattatgatt
    40741 atcggtttgt ttgttcttcg attcgttttt tccatgattt cttacggtgg gggtcttcca
    40801 ggggggattt tccttccgat attgacattg ggggctttaa tcggtagctt ttatggaaac
    40861 attatggttg atttcgggat ggatccaatc tacatcaagg attttgtcgt tattgccatg
    40921 gctggttatt tcacagcgat tggaaaagca ccgctgacag caattatttt agtgacagaa
    40981 atggttggct ctcttaatca tctgatgcct ttaggattgg ctgctcttgt tgcttatatc
    41041 gtcaatgacc ttctcggcgg taatccaatt tacgaatcat tattggaacg tctacttgga
    41101 aatactcaga ccgcctcact aaaaggtgca aggacaactt ttgattttcc agtaacggca
    41161 gagagttctt tagacggatt aatggtacga gacttcaatt ggcctaaaaa tatgctgcta
    41221 atctctatac gcagaggaaa tcaggaaatc ttgactcacg gtgatacgat catgaatgta
    41281 ggagatattt tggttatctt gactgatgaa gcaaacttgc caagaatcaa gcaggaaatc
    41341 gaggctaaat cttctatcac aaatttgaag caatcatttt aacgaacatg gaaaaactat
    41401 tttacaacaa ctattcatct acactacttg attctatatg attctataaa aaaagaagag
    41461 gctgggaaaa cccagcctct tcttttttat ttctatgctt ctgctactgc aggagctttt
    41521 ctcaataaac cataaatcac accagatacg actgctccga tagcaataaa caccaaatac
    41581 atgaccgggt gactcagtaa gaatacaacg aaaatcccgc cgtgaggagc cagtaatttg
    41641 attccgaaag ctccgactaa tccgccagtt aaagcagaac caataataaa acttggaatg
    41701 gcacgaatcg ggtcagctgc tgcaaatgga atcgatcctt ccgtgacaaa cgatagtccc
    41761 atcacgatgt tcgtcaaacc agcatcttga tctgttttgc tgaatttatt cttaaacaaa
    41821 cgtgtagcaa cgaaaattgc caatggaggt accattcctc cagccataac agaagccatc
    41881 acgacacttc cgccagttgc aacagtcgta gctaaagttg ctgtaccaaa cacataagcg
    41941 gctttgttga ttggaccgcc taaatctgcc gccatcattc cagctaataa tgcaccaagt
    42001 aaagcagcat tcgaaccact catacttgtc aagaaaccgt tcaatgcatc attgattgct
    42061 ttcataggga cattgaccag caacatcaag aaaccagtaa tcagtaagcc aaacactgga
    42121 taaaagagga tcgttttaat tccatccaat gattttggta agttcttgaa taatttacgt
    42181 aaaaatacga tgacataacc agctaagaat ccaccaatca gtgcacctaa gaatcctgca
    42241 ccgcctgtat tagctaatgc accagcagca aatccaacga tcaaaccagg gcggtcaccg
    42301 atacttgaag caataaatcc tgctaatacc ggcagcatga aaccaaatgc agcttgtccg
    42361 atttggttga accagctagc agcttgattg taattcccta gatttgataa ttgatcatgt
    42421 ggcacgccaa taaattggtc gatcatgaaa gaaagcgcaa tagcgattcc tccaccaata
    42481 acgaaaggta acatgtgtga tacgccgttc atcaaatctt tgtaaattct ttgtccgata
    42541 gtaccatccg catttccttc ttctgaactg ctttcttttc catcaccatg gaatactggt
    42601 gcagtaccac taagggctag attgatcaac tcttctggtt tacggatacc atcactgaca
    42661 ggacgattga tcagttcttt tccgtcaaag cgattcatct ctactttttt gtctgctgca
    42721 acgattacac cttctgcacg ggcgatgtct tcagcagtca atcggttttt gattccttct
    42781 gaaccgttcg tttcgacttt gatctccaca cccatttctt tcgctttttt cttcaaagca
    42841 tcttctgcca tgtaagtgtg agcgatccct gttggacaag ctgtaacggc aacaacgaat
    42901 tttctttctg gatgagcaga agcttctgca gcttgtgttt cttgttctgc tttttcttct
    42961 gcttcttttt gctcttcagc tgtttggaac aagtcttgga cttcttctgg tgtatttgct
    43021 tcttttaatt ttcctacgaa atctgcatca atcagcaatc gagatagtgc tgctaatgct
    43081 tgcaaatgtg tgtcgtttgc gccttctgga gctgcgatca tgaaaaacag ataagttggc
    43141 tgtccgtcta aggcttcata atctactcct ttttggcttt ttgcgaataa aactgttgct
    43201 tctttaactg cactgttttt cgcgtgaggc atggcgattc catcaccaag acctgtagaa
    43261 gtttgtgctt cgcgggctag aattccttct ttatatgttt cgatatcgga aatccggccg
    43321 gcatcaaaca ttttttggac catttcatcg attgcgcctt ttttatcagt cgcttgcaaa
    43381 tccatgatca tcgcttcttt gaccattaaa tccttgatat tcatgattat cctccttgaa
    43441 actaatcaat ttttgtgatt tcaacttctg gcagcaactc atcaatcaat tcgcgagctg
    43501 ccaagtcatc agaaaaagct gttgcgcttc cgcaagcaac tccccattta aaggcttcga
    43561 ttggatcttt cgtacggctg taagccccaa taaagccagc aatcattgaa tcccctgcac
    43621 ctactgaatt tttcaatggg cgaactaata cattcgtttt ataagttcct tgttccgtga
    43681 agagcaaagc accgtctcca gccattgaga tcagcacatt ctgtgcccct tcagctagta
    43741 attttttccc atacggaatg atttcttcta cagattggaa ggttgtgttg taaagctcag
    43801 ctaattcgtg attgtttggt ttgatcaaca acggtttctt aacaagtgca ttttttaggt
    43861 catcacctgt tgtatcaatc acgaaatctg cacctttttc tcgaatgata tcaatcagtt
    43921 cttgataaaa tccttttcgc aggctagctg gcgcgcttcc tgacaatacg acgatatctc
    43981 cttgtcctac tgttcctaat gcttgcttca actcatcgat ttcttcatct gaaatagatg
    44041 ggcctaagcc attgatctca gtttcagtgt ctgattttag tttgatattg attcttgtat
    44101 cttcttttac ggttgtgaac aaagactgga tttgttcttt ttcaagccac ttcgaaataa
    44161 attcgccagt gaaccctcct aaaaacccta gtgctgtaga ttgagaatcg atccgtttca
    44221 aaatacggga aacattgatc ccttttcctc caggtaactt gaagtcttcc ttcattcggt
    44281 tcaaatcgcc tagttttaac ccatctacgc gtacgatata atcaattgat ggattaagtg
    44341 ttactgtgta aatcattgta ttgcctcctt aattgttgtc ttttcacgga attcattaac
    44401 gaattctaat ggacaacgct cggttatgat cgtggctgct tccaaaggtg caacagaggt
    44461 gaaggacaac tggttgaatt tggtatgatc aatcaataca aatgtctctt cggaattttt
    44521 catggctaaa cgtttaacag ctgcctcttc tggatccgga gtcgtgaatc cttgttcaag
    44581 atgggcaccg ttcattccca taaatgcttt attaaaatga aaatttttta gttgttctat
    44641 cgcattagat cctaaaacag cattagttgc cagctttatc gttccaccta aaataatggt
    44701 agcaagctcc aaatcaacta aatttgccgc atgttgaacg gaatttgtca ctactgtgat
    44761 ctgttttcct tgcagaaaag gaatcatttc taacgtagta gagccagcgt ctaaataaat
    44821 gacatcttcg tcattaatgg tttcagctgc cgatttggcg atttgctgtt tttcgtgaac
    44881 gtttttgagt gatttttcac tcatattctg ctcaaaattc aataattgct ctttcttagc
    44941 tccgccatga actctttcta gaaacccact attctccagc tcttgtaaat cacgtcttat
    45001 cgttgattca gaagcattca aaaggtttac aagatcttgt gatttcacga ttttctgctg
    45061 ttcaagcaag tctaggattt tttgtctgcg ttcctctgta agcattttca cgatcacctc
    45121 aatacaaata atagcatatt ttttttctac tagcaatata aatcagtcaa aaacgttcat
    45181 aaaaattata aatcagtcaa aaaaaaataa aaaaccagtc agaaagactg gttttttatt
    45241 tttttgtcta tcatcaagcc gttttatcgt tttttatttt cgcttcttgt ttttttcata
    45301 taaacagaag caggttttct ttccttatga agaagataat aataaagtcc catcccgcca
    45361 aacatgacca aaaaaccgat gactgatcga ataggaaaat taggaacaaa aaagagacag
    45421 acggataata atgatgccgg aaccattatt cgataataga gttgtttatc cattttcttc
    45481 acctcatatt ttttgtaaag taatcaattg ttctaattct cggtaatcta aaaattgcac
    45541 acgcagatgc tgctcacctt tcttgacatc gaattggata cttcgccgct ttttctttat
    45601 tcgtttgaag gtaacatcag agagtctatg tttgatttcc tttaacggta attctgacag
    45661 ggattctgaa ggaacataga ttcttaagga taaaagatac tcttgatatt tttcatctgt
    45721 tacctctact tctagatatt ccctaatata ataacaataa gcttcgattc cattatccaa
    45781 agcaacagtc ggatcgataa ctttatagca catcatgcgt gatcgattct cgtcaaccca
    45841 aggaaaatac gcctgagcat ttagtgttcc gcgaacacgc ctttctttat aatctccttg
    45901 ttggatatca taactttttg ttacaaaaaa tgaagcattc tttttaggaa caggttgcat
    45961 ttgtgcattc tgatactgga cagattggat cgtatggtca gtgtaaatgt attcaacgaa
    46021 tggcggtttc aataaaccga caccattgtc ccaagcaaga tgatcaaacc caagctggtc
    46081 cctaaaatag gattgaaaca tttgattgtt tggcttttgc cagttaaagg ggtctcgata
    46141 aagctggata catatgactt cgttttctaa ttccttcact ttttctactt gttggatgtc
    46201 gagaaaaatc ggtttaggga tgatttgatg ataatagcga ctataataca tttcccaaca
    46261 cgaagtgaaa cataaacctt ggtaaaaaag atcgtatcct ggaaattggt tgcaatcgac
    46321 tactacttcc ccgttttgtt gtttcatttt aggtaaatgt tcgatttcat cctttttctc
    46381 aaacaatagc cgttgagaga tctgttttgt attgtgatag ataaactcgc tataagctcg
    46441 catataggcg aacaccccat gagcaatcat ttttttctga atgaagtctc gaatcaaaaa
    46501 ttgcttttct ttgaaaacag agatagacaa tacatttttt atcaataatt gattatcttc
    46561 caagcgaaaa ctacattcgt tctcgtcatt tcctacaact aaccaacttt cgtctcttac
    46621 cgatgctagc attatttttt cttgaagctt tcgaagatca tacggatccg cttgttgtgt
    46681 gaaagagcgg ataaatgttg gctggatcaa cagacgtgtc aaaatagccc aatcgcttat
    46741 ttcctgtttt ttttcttctt catttaagtc tgtgagctgg atagtcatca ataatttatc
    46801 ttcgccaatc attttctcac ctctttcaaa tctttttata atttaattat aaccaatgat
    46861 aaagaaaaac taaaacttta tgtcttagaa accaatttgg acgattacat gatagactaa
    46921 gtaaaaagaa acaaagaggt gtttacaatg gggttgaaat ttgaacgtac agataattta
    46981 gataaacttt tcgaatcatt tgctgtcgat ccggaaaaaa cagttgcacc aaaagaagag
    47041 atcgatcgat ttctaaaacc acagccacaa aatgaaaaga aaaaagacaa gaaacaaaag
    47101 taagttaagc tcaaaaaaag acttaccagc caaactttta tgaaagagtt tgactggtaa
    47161 gtctttttta ttctttttat gattcaattg cttgttcgat tgctgcccac gcttcttctt
    47221 ttccctgttt tgtaacggaa gaaaagagaa taaacgtgtc attgcgatca aaatccaacg
    47281 cttttttgat ttcgctctca tgcttgttcc atttaccacg agggatctta tctgccttag
    47341 tggcaacaac aattacaggg atatcataat acttcaaaaa ttgatacatc tggatatctt
    47401 cttgagaagg cttatgacgc aaatctacta aagaaacgac cgcacgtagc tggtctctag
    47461 tcgttaaata tgtctcgatc atctttcccc attttgctcg ctcagttttg gatacttttg
    47521 catacccata tcccggaaca tcgacaaaat gtaatgcgtt ctcgatcaaa taaaaattca
    47581 atgtctgggt ctttcctggc ttaccggaag tacgggctaa attctttcga tcaatcaatg
    47641 tgttgataaa agaagatttc cctacatttg atcgtcctgc cagagctatt tctggcaatt
    47701 gtgtatctgg atattgtttt ggtgccactg cacttatgat aatttcagct tgatggactt
    47761 tcatggtctc tctccttaac aaggtgatga ataaagcaag cggctgtcgc aaaagccaaa
    47821 atgactttta cggcagcctc ttttcatcaa cccgcttttt tctctgaatc tttatagatt
    47881 actgttggct caccagacaa ttcagctgct tcttttgtga taatcacttt ttcgatgttt
    47941 tcatttgaag ggatatcaaa catcacatcc atcatgattt cttcaatgat ggaacgaagt
    48001 cctcgagcac cggtattgcg ttcgattgct tttttcgcaa tggctctcaa cgcttcaggt
    48061 tcgaattcaa gttcggtatt atctagagaa agaagttttt gatattgttt gactagcgca
    48121 tttttcggct cagtcaagat ccgtaccaga tcatctgttg tcaatttctc taaagctgcc
    48181 ataacaggta atcgtccgat aaactcaggg atcaatccga attttagtaa atcttcagga
    48241 atgatatgct gcatgacact ttcgtcttca gaaagttttt ggttttgtgt accaaatcca
    48301 atcgtctttt cgcccatacg attcttcaca atcgtttcga tgccatcaaa tgcaccacct
    48361 acgatgaaca atatgttcgt cgtatcaatc tgaataaatt cttgatgtgg atgtttacgt
    48421 ccgccctgag gtggcacact tgcaacagtt ccttctagaa ttttcagcaa ggcttgttgg
    48481 acaccttcgc cggaaacgtc acgagtaata gatacatttt cgcttttacg cgcaatttta
    48541 tcgatctcat caatatagat gatccctttt tctgcaagtt ccacattgta atcagcagat
    48601 tgaagcaatt taagcaaaat gttttctaca tcttccccca cataaccagc ttctgtcaaa
    48661 cttgtcgcat ctgcgattgc aaaaggtaca ttcaatgttt ttgctaatgt ttgtgctaaa
    48721 aatgttttac cagaccctgt aggtccgatc aagcagatat tgcttttttg cagttcaaca
    48781 tcatctgtgt tttcttgtgc ttgctgattg acacgtttat agtgattgta tacggcaaca
    48841 gctaacgagc gttttgcgcg atcttgtcca atcacgtagt tattcaatac ttcaagaatt
    48901 tctagtggtt tagggacttc agtgaattcg cgtacagctt cttcataaaa ttcttcatca
    48961 atgatttctt tacataggtc gatacattca ttacagatgt agacccctgg accagctacg
    49021 atctttttca cttcttcttg ggttttcccg caaaaagaac agcgtacggt ctcattgcta
    49081 cttggattgt catacatggt cttcacccct taacttgcaa aaagtatgca agaaactatc
    49141 aaagacttta cagtctttta ctatcataac atagtttagt aaaaagaaaa agtgtttaac
    49201 aaaggatcaa ctgacctata cctttgcatt ttttactctt aacaaaaaat aaggaactgt
    49261 gacaaaatcg tgtcacagtc ccttatttcg aataaacaat tttctagaag tagcttcttc
    49321 agaaatgatt tattacttga tttctattct ttcgcttctg tcgctgtacc tgtgatcaat
    49381 tcgattgctt ttttcattgt cacatcatgt tccagcatat cttctgtcaa cacgcgtttg
    49441 atttgttcga ttggcatgtt gtatgtttct gataattctt ggatttctgc attgatttct
    49501 tcttctgttg cttgaagatt ttcagcagct gcaattgctt cgatcacaag atttgttctt
    49561 gtgcgcattt ctgcttcacc ttcgaattgt ttatgcaagt cttcttctgt agaaccagtt
    49621 aattgatagt acatttcagg agagatacct tgacgttgca tgttgttcaa gaattcgtcc
    49681 attgaacgat gtacttcatc atgaaccatc acgtgtggca attcaacgat ttctgcgttt
    49741 tcaacagcca tacggatcgc tgcttcatct tttgcatcgt ccgctgcttt ttcttttgtt
    49801 tctactaatt ctttgcgata tttttctttc aattcgtcta atgttgatac ttcgtcatca
    49861 acatcttttg cgaattcgtc atctaattca ggcacttctt ttgcttttac ttcatggatt
    49921 gtcactttga agactgcttc ttttccagca agatcttctg cttgataatc ttctgggaaa
    49981 gttacattta catctagact ttcacctgat ttatgaccaa ctaattgatc ttcaaaacct
    50041 ggaatgaatg agcctgaacc taattcaagt gagtaatttt cgccttttcc gccatcaaac
    50101 gcttcgccat ctacaaagcc ttcgaagtcg attactactg tatcgccatt ttcagctgca
    50161 gcatcttctt tgataactaa ttcagcttgt gcttcttgtt cacgtttgat acgtgcatcc
    50221 acgtcagcat ctgttacttc acggtcttgt ttttcaacaa ctagatcttt atattgtcct
    50281 aattttactt ctggtttcac tgttacttca gcagtgatta cccagtcttg acctttttcc
    50341 atgctttcaa catcgatttt tggttgagaa acaggatcga tgccagcttc tttgacagca
    50401 gcttcataag cttctggtaa tacagcattc aatgcatctt catataaagc ttcttcaccg
    50461 tacatacggt tgaatacttg acgagaaact ttccctttac ggaatccagg tacgttcaag
    50521 ctttttttca ctttattaaa cgcagttgtt aatccttttt ggatttcgtc ttgcgcaatc
    50581 gtaaatttta aaacaccatc attcgtgcct tttttttcaa atgttgcagt catttaattc
    50641 cctccgagtt tagaaattca ttttatacat caaaaacgtt tcatgcatac ctttaaatag
    50701 tacactaacc ctttcaaatt gtaaacatat tgaatggata gaatccttgt ttttcataac
    50761 atttttatct ttttcactga taaaatctaa tggaagaaga tcatttgctg caaaattttt
    50821 tctctttttt ttaaaaaatc ttgtttttca tcttctgtac cttcatacat cgtttttcct
    50881 gtacacgcct ctacagtcag ttgaaaccag cgcttcgggt cttcgatgta tgtatcatag
    50941 acaggataaa caattgcaga ttgcaacgtg aattcttctt ttaacacgga actaagcagc
    51001 gcatcttcat tcccgtaacg atctgaaaat acacataatt gctggtaact tgcctgctgc
    51061 tgcggctttg gcagatcttt tggtatgact ttgactaact tttcttcaat cgtcaaatag
    51121 aaaacaggat ggcattcgtt caattgccta agtgtctcca gtaattttgc ccttgctaaa
    51181 atatggacct ctggcgatac aataagttta ctcgctgttt ggataaactc atgataagga
    51241 agctgttcga tctcatcaat agacgcaagc tgacgtgcag gttccaacga aggaagttcc
    51301 tccgatttat tgtggagtgc cttgatcttc tgcctctcca tttgtccgga taattcttct
    51361 gcaaattgga ttttttcaat gacccgctgc tcgaaagctt cataaaaatc tgtacttgct
    51421 atcaattttc tagcatataa aaaatcttgt gtactgatta acaagtcaaa ataaaattca
    51481 gcaaactcct cattttgaag atactgtttt tcatgcaaga gtgcttgctt caatgcttct
    51541 tttttctcgt ccaactcgta aagacaaaaa acaagcaggc tattagcctc aaagctttct
    51601 ttcaactcgt aagctcgttg aaataattct tttgcctgct gatattgctg gtttgctagt
    51661 gcttgttgtg ccactcgcat taaattctca tatttttctg gaaaatcaat cggcttcacg
    51721 cagtgtcctc ctcttgctca tattcagctc atgttcaata gtagcaaaac aaaaaaagca
    51781 tgacaattaa aaataactga agcaccctct aaaagctaga ttttagatct gacttttaga
    51841 aggtgctttc aactgttact gctttttatt gctcatcagc tgttgacttc ctggttgaac
    51901 gctttgatca gattgatcgt aacaacactt gtattagtga aaaaatcgcc cagatgttgc
    51961 tttttctgat taaagactgc tggaagatac cctaacatca gcaaaggaaa ttttagaatg
    52021 aaccggcaag caccttctct aactaaaacg gttgaccagc taagttcttc ttcatcaaag
    52081 ctgatgactc gaataccaaa gatcattttc ccaatcgttt ggccatgatt gaatttcgtc
    52141 aataaaataa aatacgctag ataaataagt aaggctgtga atccgtagag actcagataa
    52201 gaatctgaaa aattaagcca acctagattt ttggcaatac caagtgttaa acgcgtaata
    52261 gctccgatac acaaaagatc gaccaagaag gcaaacattc ggataaaaaa acctgcatag
    52321 aaatacttag gaaaatcatt tgcttttggc ggttcatttc ttgtgtccgt atactttttc
    52381 catttacgtt gtttcttttt gatttcttcg ggcgtcaaag gctctctttc aaaagagctc
    52441 agtacctttt gggaaaagtt ggagtcaggt cctacagcag gctgctgttg cttatctttt
    52501 tcttgttcct tattttcatt tgaaagctct gacatgctta ctcacctccg taataataca
    52561 ttgcttttgg tgattccgct gtccctagat tctcaagaac ggataagatc tgactagtct
    52621 cagaaggctt caatccctgg aattctgcta gtttacttcc taaccatgta ctagcaaacc
    52681 cagatggcgt ggaagaatat tctactagtt ccgcatcttc aagctgttgt tcctttcgta
    52741 tagcttttaa agcatcttca ggaaatccca attcatcgac taaacctact tcttttgctt
    52801 ggacaccatc ataaatcctt ccatcagcaa tttttttgac ttcttcttca gatttattcc
    52861 gtccctcgct gacgattgct acaaaacgac tatatgcgct gtcgatatat gcttgaagca
    52921 ccgcttcttc ttcttttgtc tctggtcgcg ttcctgaacc catatctttc aacgctccgc
    52981 ttttaactgt tgtatcttcc acacccagtt tttctagaag tcctgaataa ttcaatcctg
    53041 acatgatcac accaattgag ccggtcactg tttcttctgt tgcaaagatt ttatctgctt
    53101 gagcagaaat atagtagcca ccgctagctg ccatgttttt catgctgaca tacattggga
    53161 tatcatgttc tttacgaatc gtatccaatt tcttagcgat ttccgcactt tcgtagacac
    53221 cgccaccagg agaatttact tctaaaaaga tacctttgac tgttggatct tcttcaatcg
    53281 ccttcagttg ttccataaaa tttttatgat tgtaaccttc agaagcaaat aaacctgaag
    53341 accctcccgc agcaatcgtt ccatctactg ataattttac gattttttgc gtactgtctc
    53401 cctcttccaa cacatttgga gctagttctt catccccata taaccaactg ttcaatccct
    53461 caatttgatc gttttcctga ggcttagtca aactagctga aactaacgaa accacaaata
    53521 atcctgcagc aatcagaact gctgcccaac gtcttttatt cattctttct cctttctaat
    53581 acaagttttt tatgacgatc acagtttctg actgctcaga agtgtcgaac cgctctgctt
    53641 tttcttcccg cgcatacccg gaaaacgact cttttgttaa ttcatcttct accgtaaact
    53701 cacgagcttg agcaacttcc aaggcatcag gtgcgatttc ttggatgcta tcccagccag
    53761 tatctaaaaa tattttcgta tcatctgata caccagctga tgcttcgaat ttggacaatt
    53821 gtttttttaa cattcccagt gtcatgatct caatttttga atccattttt actcctcctc
    53881 tatccgatta ctttctattg taccgaactc ctgaaaaaga tgatagataa aaacccatta
    53941 ttttttgaca taaaaaggac atttgacaaa gaatttgata tataccaatt tattttttat
    54001 tctttttttg ttgacaagaa cattcgcaaa aggttatgat tttaaatgtt tccagataaa
    54061 aaacaaaaag gaggatagtt atacctttta ggatagcgcc aggtctgtgt aaacagatga
    54121 cgaggagagg gtttatcgaa agattcggcg gatggcccta gggactgcac tctacagaaa
    54181 atccacaaaa aatgtctgca aagataaaac aaaaggattt tcctgcagct ggaagcattc
    54241 atacgaattc attatagaaa gtaggatgtt ttttatgtgc ggaattgttg gaatgattgg
    54301 attaaaaaat gtaacaccag gattgattga cggtctagaa aaattagaat accgtggtta
    54361 tgattcagct ggtatctttg tctcagatgg cacaactgat tatctggtca aagcacaagg
    54421 cagaattcaa aatttgaaaa acaaaataaa tattgatact acaggaaata taggtattgg
    54481 tcatacacga tgggcaactc acgggcaacc ttctgaagaa aacgcccatc cacatacttc
    54541 ccaaagtgga cgcttcgtgc tcgttcacaa cggcgtaatc gaaaactttg aagaacttaa
    54601 aattgcttat ttagcaaatg accactttat tggtgagacc gatactgaga ttatcgctca
    54661 tctaattgaa actttcgcga aggatactac aacgcaagag gcgtttttaa aagctttgcg
    54721 ggtcatcaaa ggttcttacg ctttcgcttt gattgatcgg acagcacctg atgttattta
    54781 cgtcgctaaa aataaaagtc cactattaat cggactcggc gatggattca atgtgattgc
    54841 cagcgatgca atggctatgc ttgctcatac gaaggaattc gttgagattg aagatgaaga
    54901 gatggttacc gtcacatcag aaaaagttat tattcaaaat tttgcaggtg acatcgtaga
    54961 acgttcttca ttcgaagcac aagtagatgc ttcagatatc gagaaaggta cctatccttt
    55021 ttatatgctg aaagaaatcg acgaacagcc tattatcatg cgtcgtatcg ctcaaaaata
    55081 cgtaacagaa gacaatcgtg tctctcttga tcaaaagctg gtcgatacat tgtctgatag
    55141 cgatcgaatc tatatcgttg cttgcggaac tagttaccat gcggggttag caggtaaaca
    55201 gactttggaa aaattgacac agattcctgt tgaggttcat ttagccagtg aatttggcta
    55261 taacacacct cttctttcaa aaaaaccatt ctttattttc ttgagtcaaa gtggcgaaac
    55321 agcagacagc cgccaagtgc tggtaaaaat caaccgtctc ggctaccctt cacttacgat
    55381 tacaaatgtt gctggttcta ctctttcgcg ggaagcatcc tttacactgc tattgcatgc
    55441 tggacccgaa atcgcagtag catcaacgaa agcttatact gcacaaatag ctgtactaac
    55501 tttattagcc aaagctatcg gtgataaaaa agaatttgaa acttctcttg tattcgatat
    55561 cactcatgaa ttgagtttag ttgctaatgc gatggaatcg gtcatcgccc aaaaagatta
    55621 tttagaagag ttggcagaag tatatctcag cgatacaaga aatgcctttt acattggacg
    55681 tggtgctgac tacaacgttt ctcttgaagc tgctttgaaa ttgaaagaaa tcagttatat
    55741 ccaggcagaa ggttttgccg ctggcgaatt gaaacatgga acgattgctt tgatcgaaga
    55801 aggaacacca gtgatcggaa tcatcagtga agaagtgact ggtgcgcata ctcgaggaaa
    55861 cctgaaagaa gtcgaaagcc gtggcgctaa aacactagtt attgtctcag agggattaga
    55921 aaaggaaagt gaccagctgg tgctaccaac tgtccaccct tatctaacta ctctaaccac
    55981 agtcgtaccg actcaattac ttgcatatta cgcaacgcta ttacggggat tcgacgttga
    56041 caaaccacgt aatttagcga aatcagtgac agtagaataa taaaagaaca aggaccagtg
    56101 tcagacatct gacactggtc cttgttcttt tattttttat gaaaatatct cttaatccag
    56161 tccgatttct tctttgacta ctgccgcgat tttatcgaca tagtaattaa ctttctcatc
    56221 agtaggcgct tctgccatca ctcgaagtaa tggttctgta ccggaaggac gaactaggat
    56281 tcttccttct gatcccattt cagcttctgc ctcttcaatc acttttttga ttgccggaac
    56341 atccatcgca ccgtttttat cacttacgcg gatattcact aatttttgcg gatagattgt
    56401 tacctctgct gcaagctctg ataatttctt acctgtctgt ttcatgatat tcagtaactg
    56461 aattcctgaa agcatcccat ctccagttgt gttaaaatct aagaaaatca tgtgcccaga
    56521 ctgctcgccg ccaaagttat aatcgttttt gcgcatttct tctacaacgt aacgatcacc
    56581 aacttgtgta acgacatctt tcaaaccgat tccttccacc gctttgtgga atcctaagtt
    56641 actcatgaca gtcgtaacga tcgtatcttt tttcaatcga tttttttctg ccaagtattt
    56701 agcacagata aacatgattt tatcaccgtc gacaatgttt ccaagttcat ccaccgcaat
    56761 aatacgatcg ccatcgccat cgaaagctag tccagcatct gctccttttt caacgaccat
    56821 ctcagctaaa cgttctggat gggtagagcc tacgccgtca ttgatattta acccgtctgg
    56881 atttgtgccc attgtataaa aatctgtttc aagatcggca aacaaacgat taacacttgt
    56941 tgaagttgct ccgtttgctg catcgacaca aatggtcaaa ccggaaaggt caccgttgat
    57001 cgtttgtaca aggaattgag aatatttcaa taagccttct ggaaattctt ctacagttcc
    57061 caatccttca gctgatggac gaggcaattc atccacttca gcatctaata atgcttcaat
    57121 ttctagctct tggtcatcta ctagtttaaa accatctgca ccgaaaaatt tgattccatt
    57181 atcttgtgct ggattatgag aagcagaaat catgacccct gcgcttgctt tttgcaaacg
    57241 tgtcagataa gcaacacctg gtgtagagat cacacctagc tggaacactt cgatacccac
    57301 cgataaaagt cctgaaatca gcgcttcttc taacatttga cctgagattc ttgtatcccg
    57361 ccctactaac acgcgtgggc gttttccttc ttcttcatgc tggcttagga cataacctcc
    57421 gcaccgtccc agcttaaatg caagttctgg cgtaagttcc ttgttggctt caccgcgtac
    57481 gccgtctgtt ccaaaatatt tacccatagt tgtaaaatta ctcctttaac ttaattttct
    57541 gaggaatttg ttctgctcat ttgtgttgat tcgcttgttt ctgatgtttc ttctgtactt
    57601 gtggtcgagc tgcttgtccc agattccgaa ctggaagaaa tggttgttgt cgatggttcc
    57661 gttcctgaat cagcagatga aatacttcta ctcgtacttg tattttcttg tgtccgactg
    57721 ctcgtcgaag cagagctact gcttgacgtt tgcaacacgg gtgtaatctc cacactaact
    57781 gattcgggct tgatacttaa cccctcttct actggtattt caattttacg agttgttcga
    57841 tgattgatac ctgagacatt gatcggtaca gcaatactgt caccgatcga tgcaagcaaa
    57901 tcgctagagc cgcttacttc tgcttccagt tttgaaaaac tgaaatcata gctttctacc
    57961 ccggcagctg gtgttccttg ctgtatccca taaagtcgta ccgacttgct tggcttgatc
    58021 gaatcagctc tcactgttac ttgcactgga tcagagacga tacttaatac ttcccctgct
    58081 gcattcagcg cctgtattgg gacctcacta ttaatcccat catctgtgac catcgaagcg
    58141 tccacatcag ctactactcg tttgatttct gccaatgttt tgtcacctgt agtgatagag
    58201 acttttttag gactaacgga agtatcatca agttcatatc cagatggtgt gaacgttgaa
    58261 ggaatcaacg tttccacatc gaaatctttt attacctttt tctcaaccgt tactgtaata
    58321 gtcgatggtt cgatctttgc tgtgacagca ctgctcaaat tttgaatttt tagcggaact
    58381 tcatgtgttc cttcgcccag cttagtgata tctgctgtga cgcggaacat ccgtgtatct
    58441 tcgtttgcct ctgcattcaa ctgaactcgg tttgcactgg aaagtttgac agtcactgca
    58501 ttttcatatc catggatata gtagcgcttt gaatcataag aaatcttgat cggcaccccc
    58561 gtaactgttt gattatacgc ttcatttcca gaaagcgtat ttggaacgga ctggccattt
    58621 gcattgaagt acagcgcaat actgaacagc aatgcaataa aactgtaaag aatgcttgtc
    58681 cgtttctctt ttgagatcat ttgcgatccc ctcccttagt cataccgtct aggaaagatt
    58741 gaatcacgtt tttcttgttt tttgttttct cttcagcaat tagttctcta cgcaagattg
    58801 ccaaatattc ttgctgggat aagccagcca tcagttcatt atctaacgtg atgctgacgt
    58861 ccccggtttc ttcagagaca acgatggtaa tcgcgtcact gacttcgctt ataccgacag
    58921 ctgcccggtg tcgagtcccg aattctttag ggatcaacat actttctgat agaggaagat
    58981 aggcgcttgc tactgcgatc tttccttctt ttacaataac tgcaccgtca tgcagcggtg
    59041 tattaggaat aaagatattg atcagcagct ccccagtgat atctgcatct aaagcaatcc
    59101 ctgtctcaat atactcatct aatccagtat gacgttcgat agtgatcaac gcaccgattt
    59161 ttcgcttgga catatattgg atggctttgt caaaagacaa gatcatcttc tcatcttcct
    59221 gctgttcgct ttttgattgt ttgaaaagcg aactgcgtcc aagatgttcc agtcctctcc
    59281 ggatttccgg ctgaaaaatc acgatagcag ctatcacccc gtacgtaatg acttggttca
    59341 tcagccaaga caaggtgtgc agaccaatga tttccgctac gattcggata acaataaaaa
    59401 tagctacacc tttcagaagc tgaatcgctt ttgtgcctcg cagcagttgg atcagtttgt
    59461 atacaagata ccagacaacc agaatatcaa gtatgttcac caaaaaattc agagagaaca
    59521 agtcaccgga aaacatttgc tgccaatagt ccaaattaaa aagttccccg atttgaaatg
    59581 acataaaaag cctctcttct cttttgagat ttcctctcta gtatatcata tgttttaatt
    59641 ttagaggctg atatgctcgt atttttcttg agaaaaacgc ataattttca agaaaattgc
    59701 aagtatgcgt tttatttttc tctgcaataa atatttttaa acttcagaga attattcatt
    59761 atattttttc tgttattttt cctttttttc ttttgtagct tgttccattt ttttacgtcg
    59821 ttctgctctg gcagctcttc tagcagctct tctttcttct gcttcaaggt ccgtcgtttt
    59881 ttcttttcta gtcttaacgg gtacatcgat agattcctct ggttcaagtc gtttcttgat
    59941 tttttctaat tctcccgtca gtgcagcata gttgtaaaaa cgtgttttag cgtcgtggac
    60001 agtcttttca aataaagctt ttgcaagatt tggatttgcg ttatctagcg aggaaaagcg
    60061 aacttgtttc ctcatgaaat caggcatagc agagaaatca ggttttttgt agtccaaagt
    60121 cattgggttc ttgttttttt cgaccattgt agggttatat cgatataatg accaataacc
    60181 agaagaaaca gcttcttttg cttctttaag cgattctttc attccgccaa gcaagccatg
    60241 tgtgatacaa ggcgtatagg caataatcag agaaggacca ggatactttt ctgcttcttc
    60301 aaatgccttg atcgtttgca tttgattggc acctgaagca atttgcgcca cataaacatt
    60361 tccatatgtc atcgccatca ttcctagatc tttctttgaa gcatactttc cactagccga
    60421 aaatttagcg attgccgaag ctggtgttgc ttttgaggtt tgaccgccag tattggaata
    60481 gacttcgtta tccaaaacaa ggatattcac gtctgctccg ctggcaagta catgatcgat
    60541 cccgccaaaa ccaatgtcgt aagcccatcc gtctccacca atcatccatt ggctgttttt
    60601 tacgaataga tcctttctct tgtatatatc ctccagttca gggaaaattg atcgttcgct
    60661 ttcgatcgca gcttgcagtt ttgctgctct ttgctgcgtt ccttcactct caaacatgtg
    60721 tactaaccag tcttccatca attctcgtag ttcttctgat acttttggca atacttcttt
    60781 gattgaactt gccaaagcct cgcgacgagt ctgtgccgct agaagcatac cataaccaaa
    60841 ttcagcatta tcttccaata aagaatttga ccatgcaggc ccttgaccac attcattggt
    60901 cgtataagga gaagcaggag aagcaccgcc ccaaatagaa gaacagcctg tcgcattcgc
    60961 gatcatcatt cgatcaccga acatttgtgt caataatttt acataaggtg tttcgccaca
    61021 gcctgagcaa gcgcccgaga attcaagaag cggttgattg aattgtgtag acaagacagt
    61081 attctttcct ttgactggat ttgctttttg tcttaatgtc attgcaaaag cccaattcac
    61141 agcttgttct ttctgttctt cataaggctt catcactaaa gctttttctt ttgcgggaca
    61201 agcctctaca cacagaccac aacctgtaca gtcttccact gaaacttgga tacggtattt
    61261 tacgccgtct gcgcctcgca tttctctcac gataaatcct tctggcgctt cttccatttc
    61321 ctcttcatct gctaaaaatg gacggatggc agcatgagga caaacaaagg cacattcatt
    61381 acacatcgta cagcgatcac taatccactc aggtacttct aaagcgacgc cccgcttttc
    61441 catagaagcc gttcctaaag gcatttcgcc agttgtcatt ccatttgcta caagtgtacc
    61501 aacagagagt tgatttcctt cctgtgcatt cactggttca agtatttctt gtatataacc
    61561 agtgttcctt ttattgattt tttcaggcat ctccaacgtt ctccaatggt ctgggatcgc
    61621 gacttgatga agcagttcgg tcgtccgatc gattgcttgt atgttttttt ctacgatagc
    61681 cattgattta tgtccatagc ttttcagggc ttcttctttt agcagcggaa gcacctcatc
    61741 aaaaggaatg atgtcagcta atttaaaaaa agccgtttcc attgccgtat tgatccggcg
    61801 tcccaaaccg acttcttggg ccaatctcat ggcattgatc gtatagaatc taatgttatt
    61861 ttcagccaag taacgtttta attttaaggg cagatgcgtt tctaattgtt cgtcgctcca
    61921 aagagtattc aataaaaaaa tgccacctgg cttcaatcct ttgaccaagt catagctatg
    61981 caggtaagct ggtgtgtgac aggcaacaag gtccgcatgc tcgacaagat acgctgaacg
    62041 tatcggcgta tctccaaaac gaagatgaga gacagtcaga ccgccagatt ttttcgaatc
    62101 ataataaaaa tagccttgcg catatttatc ggtgtgatct ccaataatct tgatagctga
    62161 tttatttgct ccaactgttc cgtcagatcc aaatccccaa aacttggcct ggaatgtttg
    62221 cggttctgtc aaatccagac tttcctttgg ttccaaagat tgataagtca catcatccac
    62281 gatccctatg gtaaaccgtt ttttgcgtat gcttgggtct tttttcaatt catcaaaaac
    62341 tgctgagatt tggtcgggag tcacatcttt agaccccaat ccgtagcgac caccaataac
    62401 agccgggcga agttcagaat catataacgc actttgaacg tctaataaga gaggttctcc
    62461 acctgcccct ggttctttac ttcgatcaag aacagcaatt gtttttactg tatgaggcaa
    62521 cttttgcacg aatgtttcca ctggaaaagg acgataaaga tggatattca aaaaacctgt
    62581 tttccgtccg tttgcattca agtaatcaat cgtttgttca atcgtctgtg caactgatcc
    62641 catagaaaca atgacttctt ccgcatcgtc tgctccgtaa taagtcacca agtcataaga
    62701 agttcctcgt aatcggttga tctctttcat atatttttga acgatttcag gtaatttatc
    62761 ataatagggg ttgatcgttt cccgttgttg aaaatggatg tctggattct ggttcgtacc
    62821 actaacagtc ggatgattgg gattcatccc tcgtttacga aatgaagcta atttctcctg
    62881 attgatcaga ggagctaatt cttcatattc caaaacttcg attttttgaa tctcgtgact
    62941 cgtacggaat ccatcaaaga aattcaaaaa agggacttct ccttcaatag aagcaagatg
    63001 ggcaacaggt gctaaatcca tcacttcttg tacactgctt tcagctaaca tagcaaatcc
    63061 tgtttgtctt gcagccatca cgtctccgtg atctccgaaa atattcaacg catttgtcgt
    63121 cactgcccga ctagcaacgt ggaaaacact tggcagtagt tccccagcaa tcttgtacat
    63181 attcggtatc atcaaaagca gcccttgtga agcggtatat gtagttgtca gtgcaccagt
    63241 tttcaatgat ccatggacca caccagctgc tcctgcttcg gattgcattt ctactacttt
    63301 tactggttga ccgaatacgt tttcttttcc tgctgctgac cattgatcta ctaattcagc
    63361 catggtcgaa ctgggtgtta ttggatatat tgcagcaatc tcggtaaatg cataagaaat
    63421 atatgcggct gccgtattgc catccattgt tttcattttt cgcatgattc atcatccttg
    63481 ttcatattct ctcttattat ctgttccatt ataacgtaaa acatgccatt tttttctttt
    63541 atttataaaa atattttaca tgtatctatt tgaattggtc atctaatttt tagacaaaca
    63601 gtctaaatcg ttgccttttt ttgtcagaca tgtataaact ataagcgaaa gaggtgtttt
    63661 aacatggata tatcagtgat tgatgcaacg aaaacaaata cacaaaaagg aatcctttat
    63721 ggttcatcga atgctcctaa aaaaatggtc gaattcatca acttagcttg tccgtattgc
    63781 cgacaatggt ttgaagaatc ttatgagcta ttagaagaag cagtccaaag cgggcaattg
    63841 cagcgagtga tcaaattgtt cgataaagaa aaagaaagct tacagcgagg gaacgtgatg
    63901 caccggtatc tcacaatcag cgatggccaa aaagcgatca aagagatcaa acaaatattc
    63961 gatactcaag acgagtggaa acatctttct ctccaagaag tagcggattt tgcagtagat
    64021 aaactaaagc tgacagaaca aaaagacgaa cagctgagcc aagctgtcat caacgaagct
    64081 gagcaagcac atattcgatt tgttccaact gtcatcttgg gtaaagaaat ctttgatgaa
    64141 tctatttcaa tagaggaatt aaaagaattg atccaaacaa aataaaaaaa taaggagcgt
    64201 g
//
LOCUS       DACCCS010000008        60490 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.008, whole genome shotgun sequence.
ACCESSION   DACCCS010000008 DACCCS010000000
VERSION     DACCCS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.008"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            47..679
                     /locus_tag="IVK82_000546"
     CDS             47..679
                     /locus_tag="IVK82_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP6391631.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(681..1586)
                     /locus_tag="IVK82_000547"
     CDS             complement(681..1586)
                     /locus_tag="IVK82_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6391632.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1671..2324)
                     /locus_tag="IVK82_000548"
     CDS             complement(1671..2324)
                     /locus_tag="IVK82_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP6391633.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            2646..3590
                     /gene="mvk"
                     /locus_tag="IVK82_000549"
     CDS             2646..3590
                     /gene="mvk"
                     /locus_tag="IVK82_000549"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP6391634.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            3583..4560
                     /gene="mvaD"
                     /locus_tag="IVK82_000550"
     CDS             3583..4560
                     /gene="mvaD"
                     /locus_tag="IVK82_000550"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP6391635.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            4573..5658
                     /locus_tag="IVK82_000551"
     CDS             4573..5658
                     /locus_tag="IVK82_000551"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP6391636.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            5655..6719
                     /locus_tag="IVK82_000552"
     CDS             5655..6719
                     /locus_tag="IVK82_000552"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP6391637.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(6968..7771)
                     /locus_tag="IVK82_000553"
     CDS             complement(6968..7771)
                     /locus_tag="IVK82_000553"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP6391638.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(7791..8633)
                     /gene="kduI"
                     /locus_tag="IVK82_000554"
     CDS             complement(7791..8633)
                     /gene="kduI"
                     /locus_tag="IVK82_000554"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP6391639.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(8649..9656)
                     /locus_tag="IVK82_000555"
     CDS             complement(8649..9656)
                     /locus_tag="IVK82_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP6391640.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(9663..10307)
                     /locus_tag="IVK82_000556"
     CDS             complement(9663..10307)
                     /locus_tag="IVK82_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP6391641.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(10407..11165)
                     /locus_tag="IVK82_000557"
     CDS             complement(10407..11165)
                     /locus_tag="IVK82_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP6391642.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(11292..12443)
                     /gene="fucO"
                     /locus_tag="IVK82_000558"
     CDS             complement(11292..12443)
                     /gene="fucO"
                     /locus_tag="IVK82_000558"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP6391643.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(12525..12836)
                     /gene="rhaM"
                     /locus_tag="IVK82_000559"
     CDS             complement(12525..12836)
                     /gene="rhaM"
                     /locus_tag="IVK82_000559"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP6391644.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(12838..13683)
                     /gene="rhaD"
                     /locus_tag="IVK82_000560"
     CDS             complement(12838..13683)
                     /gene="rhaD"
                     /locus_tag="IVK82_000560"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP6391645.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(13697..14953)
                     /gene="rhaA"
                     /locus_tag="IVK82_000561"
     CDS             complement(13697..14953)
                     /gene="rhaA"
                     /locus_tag="IVK82_000561"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP6391646.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(14950..16410)
                     /gene="rhaB"
                     /locus_tag="IVK82_000562"
     CDS             complement(14950..16410)
                     /gene="rhaB"
                     /locus_tag="IVK82_000562"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP6391647.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            16563..17432
                     /locus_tag="IVK82_000563"
     CDS             16563..17432
                     /locus_tag="IVK82_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6391648.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(17558..18457)
                     /locus_tag="IVK82_000564"
     CDS             complement(17558..18457)
                     /locus_tag="IVK82_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6391649.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(18622..19446)
                     /locus_tag="IVK82_000565"
     CDS             complement(18622..19446)
                     /locus_tag="IVK82_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP6391650.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(19457..20752)
                     /locus_tag="IVK82_000566"
     CDS             complement(19457..20752)
                     /locus_tag="IVK82_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP6391651.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(20768..21247)
                     /locus_tag="IVK82_000567"
     CDS             complement(20768..21247)
                     /locus_tag="IVK82_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP6391652.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(21253..22281)
                     /locus_tag="IVK82_000568"
     CDS             complement(21253..22281)
                     /locus_tag="IVK82_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP6391653.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(22298..23404)
                     /locus_tag="IVK82_000569"
     CDS             complement(22298..23404)
                     /locus_tag="IVK82_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP6391654.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(23633..26230)
                     /gene="adhE"
                     /locus_tag="IVK82_000570"
                     /gene_synonym="adhC"
     CDS             complement(23633..26230)
                     /gene="adhE"
                     /locus_tag="IVK82_000570"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP6391655.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(26536..26916)
                     /gene="yajC"
                     /locus_tag="IVK82_000571"
     CDS             complement(26536..26916)
                     /gene="yajC"
                     /locus_tag="IVK82_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP6391656.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(27013..28158)
                     /gene="tgt"
                     /locus_tag="IVK82_000572"
     CDS             complement(27013..28158)
                     /gene="tgt"
                     /locus_tag="IVK82_000572"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP6391657.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            28376..29074
                     /locus_tag="IVK82_000573"
     CDS             28376..29074
                     /locus_tag="IVK82_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP6391658.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(29242..31881)
                     /locus_tag="IVK82_000574"
     CDS             complement(29242..31881)
                     /locus_tag="IVK82_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6391659.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(32500..33531)
                     /gene="queA"
                     /locus_tag="IVK82_000575"
     CDS             complement(32500..33531)
                     /gene="queA"
                     /locus_tag="IVK82_000575"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP6391660.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            33724..34329
                     /locus_tag="IVK82_000576"
                     /pseudo
     CDS             33724..34329
                     /locus_tag="IVK82_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(34482..35630)
                     /gene="tnpB"
                     /locus_tag="IVK82_000577"
     CDS             complement(34482..35630)
                     /gene="tnpB"
                     /locus_tag="IVK82_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP6391661.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(35647..36051)
                     /locus_tag="IVK82_000578"
     CDS             complement(35647..36051)
                     /locus_tag="IVK82_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP6391662.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            36209..36790
                     /locus_tag="IVK82_000579"
                     /pseudo
     CDS             36209..36790
                     /locus_tag="IVK82_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
     gene            36794..37435
                     /locus_tag="IVK82_000580"
     CDS             36794..37435
                     /locus_tag="IVK82_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6391663.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37432..38349
                     /locus_tag="IVK82_000581"
     CDS             37432..38349
                     /locus_tag="IVK82_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6391664.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38346..39017
                     /locus_tag="IVK82_000582"
     CDS             38346..39017
                     /locus_tag="IVK82_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6391665.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39558..40046)
                     /locus_tag="IVK82_000583"
     CDS             complement(39558..40046)
                     /locus_tag="IVK82_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP6391666.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(40247..40513)
                     /locus_tag="IVK82_000584"
     CDS             complement(40247..40513)
                     /locus_tag="IVK82_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP6391667.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40513..40776)
                     /locus_tag="IVK82_000585"
     CDS             complement(40513..40776)
                     /locus_tag="IVK82_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6391668.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(40769..41041)
                     /locus_tag="IVK82_000586"
     CDS             complement(40769..41041)
                     /locus_tag="IVK82_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391669.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            41252..41437
                     /locus_tag="IVK82_000587"
     CDS             41252..41437
                     /locus_tag="IVK82_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6391670.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41579..43402)
                     /locus_tag="IVK82_000588"
     CDS             complement(41579..43402)
                     /locus_tag="IVK82_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP6391671.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(44000..46705)
                     /locus_tag="IVK82_000589"
     CDS             complement(44000..46705)
                     /locus_tag="IVK82_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP6391672.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(47011..47958)
                     /locus_tag="IVK82_000590"
     CDS             complement(47011..47958)
                     /locus_tag="IVK82_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP6391673.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            48148..48417
                     /gene="rpsN"
                     /locus_tag="IVK82_000591"
     CDS             48148..48417
                     /gene="rpsN"
                     /locus_tag="IVK82_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP6391674.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48593..48955)
                     /locus_tag="IVK82_000592"
     CDS             complement(48593..48955)
                     /locus_tag="IVK82_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP6391675.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48966..50087)
                     /locus_tag="IVK82_000593"
     CDS             complement(48966..50087)
                     /locus_tag="IVK82_000593"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP6391676.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(50103..50453)
                     /locus_tag="IVK82_000594"
     CDS             complement(50103..50453)
                     /locus_tag="IVK82_000594"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP6391677.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50583..52094)
                     /locus_tag="IVK82_000595"
     CDS             complement(50583..52094)
                     /locus_tag="IVK82_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP6391678.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52416..53768)
                     /locus_tag="IVK82_000596"
     CDS             complement(52416..53768)
                     /locus_tag="IVK82_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP6391679.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53921..54997)
                     /locus_tag="IVK82_000597"
     CDS             complement(53921..54997)
                     /locus_tag="IVK82_000597"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP6391680.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            55193..56938
                     /locus_tag="IVK82_000598"
     CDS             55193..56938
                     /locus_tag="IVK82_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP6391681.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(57218..57655)
                     /locus_tag="IVK82_000599"
     CDS             complement(57218..57655)
                     /locus_tag="IVK82_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP6391682.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57639..59012)
                     /locus_tag="IVK82_000600"
     CDS             complement(57639..59012)
                     /locus_tag="IVK82_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391683.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59334..59822)
                     /locus_tag="IVK82_000601"
     CDS             complement(59334..59822)
                     /locus_tag="IVK82_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6391684.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59844..60446)
                     /locus_tag="IVK82_000602"
     CDS             complement(59844..60446)
                     /locus_tag="IVK82_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP6391685.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17260 a        13020 c        11006 g        19204 t
ORIGIN      
        1 gattcccgct tctttgctat actaaagaaa aacgaggtga acccacatga aaaaaatcga
       61 acctattcca ccttctcttc ctcttcttac aaagggagag ttttatttag aagatgagga
      121 gatcttctct ggtcttctga tcaacggggg tgaccaaagc tatctcgatg ccaaaaatat
      181 ggtattaaga gagagtcata tccaaaaact tacgatgcaa aaaacagttt tacgtcattt
      241 tgaatgcagt aatgtcattt ttgaaaaatg tgatttttcc aatctggagt ggattggtgg
      301 cagtttccac caagtcgtgt ttcatcaatg taaattaaca ggtacgaatt ttgccgaaag
      361 ttatcttcga gactgtactt tcactgattg tattgcttcg atggcttcct ttagtagtac
      421 aaacttgaaa gctgtctcct tcgatcattg tcaattagaa gacagcgaat tttatgaggt
      481 cacttggaaa aacctttttt tgtctgacaa tcagttgacc ggttcgaact ggtttcgaac
      541 ttctctgaag ggattagact ttacgactaa tttcttcaca gcaatcgctt tatctcaaga
      601 ttctttggcg ggactaatcg tcaaccaaga gcaagcactt gtgatcgccc aagcccttgg
      661 tttgcagatc aaagactaga tcaacgaatc cgccgccaca gtgcaataag catccaaata
      721 gataagacca aactgatgat caaggcgatt ccccatccga aaacggaatg ggtaaagggt
      781 aactggagat tcattccgaa gaatcctgtc acaatagttg gaacggtcaa gatcagcgac
      841 cagaccgtca aaaatttcat cgtgtcattc aagttgttat tcagcaaatt attataagtg
      901 ccagagagct ggtctaaaat ctgcgaagct aagttcgtca tttcaactgc ttgtcgtgct
      961 tcgatcagcg catcgtccaa ctcttctttt tccttttctg acaacttgcg ataaatagcc
     1021 aatgccttga tttgttctaa aagtactaca ttttgttttg ttcctgtaac taagtaaacc
     1081 aaaccgatct ctaaatctga aagttgcagc agattcttgt tcgttgtttt ttctcttaat
     1141 ttcagattaa gccgctgacg ttcactattc acttcctcga ttaaaggaaa aaaggaatca
     1201 gaaatcaaaa acaatgtacg aaacaataaa ctgtatacac tcatgtctgg cattcgttcc
     1261 aaaagcgatc gaattagtcg aatcacgtac tcggtattcg gcgtggtaaa tgtaaacaaa
     1321 ccgtcttctt ttaaaataaa agcgatagga ctagtttcat aatgattatc gactttttgc
     1381 tggtgcggaa catttgatac aagcagtaac gcttcatcaa aagaatcgta ctctacgcgc
     1441 gcccgttcat ttttatctag agaataagtc agcatctcgt cactaatgcc atattcttct
     1501 tttaagtttt taacagctag cacatcgttt tgctgtacat tgatccaaaa attacggtct
     1561 tgattaaaat agtgtttttc ccgcatactc tatcctcctg acatttgttt ccagtataaa
     1621 gaaaaaagtc gctcaaataa aggtttaccc tttatttgag caactgtaaa tcaaatggct
     1681 actttggttt ggcgaatctc tgctaatttt tcttcgatca ttttcaagac ttcttctttt
     1741 gcttgaggat cttccacaaa attatagcgg tctccatcga tttggatttt tggactttct
     1801 ttgtattctt cataccattg atcgtaacgt ctgttcaatt ctttgtaata atcgtataga
     1861 cttggatcaa aggacaattg ttcatacgaa cgaccacgtt tttcgatacg ctccaacatc
     1921 gtatcaaaag atacacgaat atgaactaat aaatccggat gttttttatg ggcagcatag
     1981 ggaagttctt ccatcatgtt ttccagcagt tcatcataca cacgaacttc tgtttctgtt
     2041 gctcttccta gatctgcatt tagatggaaa agcagtgaat cttcataaat cgaacggtcc
     2101 aatacattat tgtcatcttt cattgcttgt ttgatgcttg caaaacgttt attcaaaaaa
     2161 taaatttgca gcaaaaaagc atactgttca gggtttgcat aaaataaggg caagacttca
     2221 ttatcatcaa tcgattcata aaatgcctga ctatcaaagt gttcagccat catttctgtt
     2281 aaacttgatt ttccggcacc aattgtacct gctaaaacga tcacgctcat ttacctccag
     2341 ctcacttcat cactaaatat tgtgtttaac tccaaccttt ttaaatatac ccccaatatc
     2401 tagtagtgtc aattgtattt ttttctagtc aaagaagaaa aaaagtcaaa aaattcttaa
     2461 gaaaaaagtg ttttttcttt agaaaacgta ttcttagttt tacttctgag atggttttag
     2521 taaaataaag gaggaatgtt ttttgagacc cttagtggac aaaaaaattc gtgaagcagc
     2581 gcaactcgtt ttgggcgctt tattttttgc ttaaaaatca cttttacgtg agaaggagtt
     2641 agattatggc aaactatggc caaggagaat caagcggaaa gatcatattg atgggcgagc
     2701 acgcggttgt ttatggagaa ccagcgattg cctttccttt ctatgcaaca aaagtcaccg
     2761 cattccttga agagctggat gcaatggacg atcaactggt ttcttcctac tattcaggaa
     2821 atttagccga agctcctcat gcattaaaaa atatcaaaaa attattcatt cacttaaaaa
     2881 aacagcatga catccaaaaa aacttgcaac tgaccattga aagcacgatt cctgctgaac
     2941 gtggaatggg atcaagcgct gcagtcgcca cagcagtcac tcgtgctttt tatgattact
     3001 tagcatttcc tttgtctcgt gaaatactat tagaaaatgt ccagctttcg gaaaaaatcg
     3061 cccatggtaa tcctagtgga atcgatgcag ccgctactag cagcttgcag ccgatttatt
     3121 ttacaaaagg gcatcctttc gactactttt ctttgaacat cgatgctttt ttgattgtcg
     3181 ctgatacagg aatcaaagga caaacaagag aagccgtcaa agatgtcgct cacctctttg
     3241 aaacccagcc tcatgaaact ggacaaatga tccaaaaatt aggatacttg acgaagcaag
     3301 caaaacaagc aatcattgaa aattcaccag aaacgttggc acagacaatg gatgaatcac
     3361 aatcacttct ggaaaagctg acaatcagca atgattttct taatctattg atccaaacag
     3421 caaaagatac cggggcattg ggtgccaaat taactggcgg tggacgcggt ggctgtatga
     3481 ttgcattagc acaaacaaaa acaaaagccc aagaaatcag tcaagcactt gaagatgcgg
     3541 gtgctgctga aacttggata caaggattag gagtacatac ctatgtttaa aggcaaagca
     3601 cgcgcatata ccaacattgc tctaatcaaa tattggggta agaaaaatga agaactcatc
     3661 cttccaatga acaacagcct ttcgttaaca ttggatgcat tttatacaga gacagaagtc
     3721 atcttttctg atagctatat ggtagatgaa ttttatctag atggcacctt gcaagacgaa
     3781 aaagcaacaa aaaaagtcag tcagtttctt gacctttttc gtaaagaagc cggcctctca
     3841 ctaaaagctt cagtaatcag tcagaatttc gttcctaccg cagctggatt agcctcctct
     3901 gccagcgggc tagctgcttt agcaggcgct tgcaatactg ctcttaagct tggattagat
     3961 gatctctctc tttcaagatt tgctcgacgc gggtctggtt cagcttgccg aagtattttc
     4021 ggtggtttcg tcgaatggga aaaaggccat gacgacttaa gttcttacgc taagccagtc
     4081 ccttccgatt ctttcgaaga cgatttagca atggttttcg ttttgatcaa cgaccagaaa
     4141 aaagaagtgt ccagcagaaa tgggatgcgt cggacagtcg aaacatccag tttttatcaa
     4201 ggctggttag attccgttga aggggatcta tatcaattga aacaagcaat caaaacaaaa
     4261 gatttccaac ttctcggaga aacgatggaa agaaacggac taaaaatgca tggaacgact
     4321 ttagctgccc agcctccatt cacttactgg tctcctgaca gcttaaaagc gatggatgcg
     4381 gtgcgacaat tgagaaagca aggcattcca tgctacttta cgatggatgc tggccctaat
     4441 gtcaaagttt tagtggaaaa cagccattta tctgaggtac aagaaacatt taccaaatta
     4501 tttagcaaag aacaagtaat tactgcacat gctggtccag gaattgcgat tattgaataa
     4561 agggagattt ttatgattga agtatctgca ccaggcaaac tctatattgc cggagaatat
     4621 gcagttgttg aaacaggcca tccagcagtt atcgctgcag tcgatcaatt cgtgacagtc
     4681 actgtagaat ctgcacgaaa agtcggaagt atccaatctg cacaatatag tgggatgcct
     4741 gtacgttgga caagacgcaa cggagaatta gttttagata ttcgggaaaa tccttttcat
     4801 tatatccttg ctgctattcg cttgactgaa aagtatgcac aagaaaaaaa cattctttta
     4861 tccttttatg atctgaaagt aacaagtgag ttagacagtt caaatggccg gaaatatgga
     4921 ttgggttcta gcggtgccgt gaccgttgca acggtcaaag ccttgaatgt tttttacgcg
     4981 ttgaatttat ctcagttgga gattttcaag attgcagcac tagccaattt agcagttcaa
     5041 gataatggtt cctgcggcga catcgctgct agctgttatg gtggctggat cgctttctca
     5101 accttcgacc atccttggct ccaagaacaa gaaactcagc attctatcag tgagttactt
     5161 gccctggatt ggccaggtct atccattgag ccattgattg ctcctgaaga tttacgttta
     5221 ttgattggtt ggacgggtag ccctgcctct acttctgatt tggtcgatca agttcaccgt
     5281 tcgagagaag ataaaatggt ggcttatcag cttttcttaa aaaatagtac agaatgtgtc
     5341 aacgaaatga tcaaagggtt taaagaaaat aatgtaacgt tgatccaaca gatgattcga
     5401 aaaaaccgac aattactgca tgatttatct gcaatcactg gggtcgtcat cgaaacgcct
     5461 gctttgaaca aattgtgtaa tttagctgaa cagtatgaag gagccgcaaa atcttctggt
     5521 gcaggtgggg gcgattgcgg aatcgtaatt gttgaccaga aatctggcat tcttccttta
     5581 atgagtgcat gggaaaaagc agaaatcact ccactgccgt tacatgtcta tagcgatcaa
     5641 agaaaggaaa accgatgaat cgaaaagacg aacatgtttc actagctaaa gcctttcatg
     5701 ataaacaaaa aaacgaattt gacttcgtac ggatcatcca taaccctttg ccgcaaatag
     5761 cagtttctga tgtcgatcta agtacacagg cagttgggtt tacactaagc agcccatttt
     5821 atatcaatgc gatgactggc ggaagcgaaa aaacaaaaaa aatcaaccaa gatcttgcaa
     5881 tcgtcgcaag agaagcagat ttgatgattg ccactggttc agtcagcgct gccttgaaag
     5941 tcccttcttt agccgatact tatacgatta tgcgacaaga atatcctcac gggaaaatca
     6001 ttgccaacat cggtgcaggg acttctgtcg aaagagccaa agaagctatc cgattgtttc
     6061 acgcagatgc cttacagatt catttgaatg cacctcaaga gttggttatg cctgagggag
     6121 accgtgattt taccaattgg aaagtactta ttcaagaaac tcaaacagcc attgacgtcc
     6181 ccctcatcgt aaaagaagtc ggttttggca tgacaagaga aacgctcaat gatctagctg
     6241 ctttaggagt tcatacagtt gatatcagcg gtcgaagcgg aacaagcttc acccagatcg
     6301 aaaacgcccg tcgctccaag cgggaactga gttatttagc tgactggggg caatcgacag
     6361 tctcttcatt gcttgaagca aatgaagcag acacttctat ggaaatccta gcctccggag
     6421 gtatccgcaa tgcctatgac atatttaaag ccctttgtct cggcgcaaat gccgtgggta
     6481 cttcaggaac cgtactgacg catctgatga atcacggcgt agaagaaact attatactga
     6541 tgaagcaatg gcaagaagag cttcgtctgc tttatactat ggtcggcgca acgaataccg
     6601 cggccctaca ccaacaatct ctgatctttt ccggaccagt gaaggattgg tgcgaagcaa
     6661 gagggatcga cttagtgaag tatggaagac gcacagaaaa gagcgtggga caaaagtaaa
     6721 gaacactttt gtctcacgct ctaaacacgt acaaacggcg gaagtaaaag caactccttc
     6781 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattc cttcttattt
     6841 cttggagtta aatgcttctg tcccagcctc ttttttcgaa taaatgatgt ttaatcagct
     6901 gtccttcggt attcgttcaa tccgctgatt tcacaacctc tccactaaac agtgtactaa
     6961 agctctttta aggttgtttt ccgatatagg caagtatgcc tccgtccaca tacaagacat
     7021 ggccgttaac gaaatcagat gcgctagaag ccaagaagac agatggtcca gccaaatcga
     7081 ttggctcgcc ccagcgtgct gctggcgtac gtccaacgat aaattgatcg aatggatgac
     7141 gttcaccgtt ttcttgtgtt tcgcgtaatg gagcagtttg cggtgtagca atataacctg
     7201 gtccaattcc attacattgg atattgtatt gaccgtattc agaagcgatg tttttcgtca
     7261 gcatttttaa tccgcctttt gccgcagcat aagcactgac cgtttcgcgt cctaattcac
     7321 tcatcatgga gcagatattg atgattttac cgccgccttt ttcgatcata tccggaataa
     7381 ctgcttttga aacgatgaat ggggcattta aatccacgtc gattacttgg cggaagtctg
     7441 ccgcatccat ttcgatcatc ggtgtccgtt tgatgatccc tgcattgttg actagaatat
     7501 caactgaacc tacatcttct ttgatctgtt gaaccatcgc ttgaaccgct tcttcatcgg
     7561 tcacatcgca gacataacct tttgcatcga tgcctgcttc tttataatgc gctatacctt
     7621 catcaactga ttcttgattc aagttattaa atacgatttt tgctcctgct gtagctaaag
     7681 agcgtgcaat ctcaaatcca ataccataga cagcaccggt gactaatgct acttttccat
     7741 ctaaacgaaa catatccata ctaaattcca tcattttctc tcccttatgc ttattttaat
     7801 tgatccattt gcaccatatc catgtctgta taggtgatat tttctccgca cattgcccag
     7861 atgaatgagt aattgcttgt acctacacct gaatggatcg accagcttgg cgaaataatg
     7921 gcttgttcat tgtctacaac taaatgtttt gtttcatctg gtttgcccat catgtggaac
     7981 acacgtgtat cttcattggc ataatcaaag tagacataag cttccatacg acgttcatgt
     8041 gtatgacaag gcatcgtatt ccaagcgctt cctggttcta aaatcgtata acccatttgt
     8101 aattgacagc tttcgcatac atttggatgg atgtattggt agattttacg ttcattcagt
     8161 gtcaggcctt caccagtctc cattggtttt acttggtcga tactgatttt tacatttgga
     8221 tatttatgat gagctggaac agaactaata tagaatttag ctgggttttc agcatcttga
     8281 gaagaaaata caacatgtcg tgtttctttt ccgatatagt atccgtcttg ttttttcatt
     8341 tcttcttttt gcccatcgat ctcgatgaat cctggtccac cgatattgat cacacctagt
     8401 tcacgacgct ctaagaagta atcaacacct agttctttgt ctaatgtgat ttctaattct
     8461 ttttcagtag gtgtcactcc accaaagatc attcgatcgt tgtgcgtata agtcaaactg
     8521 attttccctg gttcaaatac cttttctact aaaaattctt ttctcaattg ttccgttgaa
     8581 taatgacgga tatcttctgg actgtgtgta taacgtgttt ccatttcttg cattttcttc
     8641 tactccttct atcgaatgat ttttccagag ccgcatgcta aaaattgtgc gacttcttct
     8701 tgactaaatt ggttgcagtc cccatggatc gtatgcttca aggcagaagc tgccgtagca
     8761 aaatcaatga tttcttgcgg agacatattg gataaaatcc catggagcac gccacctgca
     8821 aaagcatctc cgccacctac tcgatccacg attggggtga ttctatggac ttgggattcc
     8881 acatattcat ggttcatcca caatgtcccc ttcaattcat tttcacttgc tgaatgaacg
     8941 gtacgcttgg tcgaataaaa cacctgaata ttcgggaact tttcctgcat tttttgataa
     9001 taataaatta gaggttgttc ttctgcttct ggcgctttct cgatacctaa caaataaaga
     9061 gcatccattt ttccagctga gcagatatct actaaaggta aaagctgatg gattacttcg
     9121 ctggcttctt gctgtgtcca taattttcca cgatagttga tatcgaagct gatcaggcag
     9181 ccagcttttt ttgcagcttc tatcacctgt ttcgtcattt tttgccattt tttagacaaa
     9241 gctggggtga ttcctgaaac atgaaatata tctattcctt ggaataactc ctctagcgac
     9301 cattccaaat gatccatttc agcaaaactt gatccggcac gatcatagat cactgaggcg
     9361 gcgcgttcgc cactaccagt ttccatataa taagtaccta gtcttgatcc accagataat
     9421 acctgtttcg tatctactcc ataacattgc aagtgctttt tgactgcttt tcccaatgcg
     9481 ttatcaggta cttttgtagc aaaaatcgct tcatgtccgt aattagctaa agaaattgct
     9541 acgtttgctt cccctccgcc ataatgggca gcgaatcgtt cacttgaatc gatacgggtt
     9601 ccctgctctg tggacaatcg caacatgatt tctccgagtg tcactacttt tcccataagc
     9661 agctacctct tgatttcttt catttttgct gcatactgtt gcgcgacttc cgttactttg
     9721 tcaaaatctc ctgtagctgc gggtgctaaa aggttcccac ctactccgac tgttacgaca
     9781 ccagcatcga accactcttt catattgtcc agactcacac cgccagttgg catgatattg
     9841 acttgaggca tcggcgcctt gaaggcagaa atgatacttg gtccataagc gcttccagga
     9901 aatagtttga cgatatctac cccgcttttc agcgctgttt tgatctcggt aatagtcata
     9961 catcctggta agtaaggaat ttgatacaga ttgcacatct ctgccgtttc ttgatcaaag
    10021 gaagggctaa caatatactc tgcgccagct aagatagcca agcgagctgt gacagcgtct
    10081 aatactgtgc ctgcgccaat cactatttct ggttggtctt tgtagaatga caaaagttct
    10141 ttgattactt gatctgcttg agggactgta aaagtcaact caataccagt tagaccacct
    10201 ttgatgattg cgtggcaagc attcaaagct tcttctttgc tttttccgcg taccaccgca
    10261 atcactccgg cgtttttcaa tcgagataaa atatcaattt ttttcatctt ctccacctat
    10321 ttcctattta gaaattttat tttataactt ggaaatctat acccatatta tacggcagaa
    10381 aagaaaaatt gcaaacgctt ttttcattat ttttgaattc tttgctcgat ttcgcttttt
    10441 gtttgcttga gggcactgat tatttgttct tttgtctcat ctgttaaacg atatttcggc
    10501 atacttacac taaatgcacc aatgatttgt tcgtttttca cgattgaggc tcctatacaa
    10561 aaaatatcac gttcgatttc ttcatcgtca aatgcgatgt gggtttcttt taccgttttg
    10621 atttcctttt tcaaccgtaa tgcattggtg atcgtatatt ctgtgtaagg gatcaacgga
    10681 tgcttttcaa gataatcagt gtactgctct tcactgaatt ctgctaatac ggctttgccc
    10741 atcgcagaat tgtataatgg tcgggtgatt ccgatttttg aagacatgcg gatcgtttga
    10801 tgttttggat ctagtttgtt cacgtacaag atctcattgt tgtccaatac ccctaagtgg
    10861 atagtctcat cgatcttttg ctgcagctgt tctaaaaacg gtaaagtcaa ctctaccaaa
    10921 tcgatttgtt caatgttttt attcgcgtag cgaataagct ttgcacctaa acgatagttt
    10981 ttctctgaat ttttatttac gtagccgatc atcaacaaag tatctagtat tttcaagact
    11041 gtagaagagg tcattcccac accctttgcg atcgtctgca aagagacatc ttggttttcc
    11101 gctaaatagt ctagtatttt agaggctttg atcaatacag ttccataagg ctttttattt
    11161 tccatataat attctcctat ttctttataa gaaaaattat agcggaaata ccatcaattt
    11221 aaaagagata tccagatata aactaccatt tctctttaca ggcaatttaa tgttgaagat
    11281 ccgcttcgtt tttacatcaa actttgatat aaagcaatga tttcttctac tgtcgtttct
    11341 cttggatttc ctggtgtgca tacatctcgc aaagcatctt ctgcaattgc tggaatatct
    11401 gcttctttta cacctaattc agaaatggtt tctgggattc caaccgcttt gctcaaccga
    11461 tcgatctcac cacaagccgc ttctcttaca tcttctaaag acatttcagc tgctccttta
    11521 atccctagaa caagagcgat ctcacggtac ttttctccag tatattcttt gttatatttc
    11581 atgacagttg gcaaaagtgc tgcacacgct acgccatgag gaatgttgta ccatgcactt
    11641 aacgggtgag ccatcccgtg taccagtcct aaaccgacat ttgagaagcc cattcccgca
    11701 atatactgtc ctaatgccat tttttctcgc ccattttgat cgccatctac agaagcttcc
    11761 aaagaatggc cgatgatttc gattgctttt aaatgcagca tatcagtcat ttcccaagcg
    11821 ccttttgtga taaacccttc aatagcatgc gtcatagcat ccatacctgt tgatgcagcc
    11881 aattttttcg gcattcccat catcatatcg ctgtcgatga acgcgacgat tgggatatca
    11941 tgaggatcaa cacaaacaaa ttttcggtga tttacttcat ctgtgatgac gtagttgatc
    12001 gttacttctg ctgctgttcc agaagtcgtt ggtatagcta atataggtaa acatggattt
    12061 ttcgtatcag caacaccttc taaactaatg acgtctgaaa actctggatt cgtcaaaata
    12121 atcccaatgg cttttgccgt atcgattggc gaacctccac cgattgcgat caaacagtct
    12181 gcctctgcct ctttaacaaa agccactcca tcttttacat tttggatcgt tggattaggt
    12241 acgatcccgt cgtataaagc ataagcgatt gctgctttat ctagaagttc tgttactttt
    12301 gttgctatac cgtgctcgat caatcctttg tcagtaatga cagctgcttt gccgaatcct
    12361 cgctgctgaa attcagggac gatcgactca atcgctcctt ttccaaaata cgatgtttca
    12421 tttaaaatca ttcgattact cattttctat tcctcctaat ttgtattatt tttcacaata
    12481 aaaacgaaag aaccccaagc aaccggttgc ccagttgctt gttgctacag ttcaaagact
    12541 ggcatcaatt cccaactgac tggagaggca tctggatttg tctccatgat atcttccatg
    12601 tacttccacc atttttgatt gatagatgta tttgccattt tttcccataa cttttcatct
    12661 tcgatcacta agtaaccaaa taacaaactt gtactcggat caagatagat cgaatagtcg
    12721 atgaccccgt gttcacgaag tgctgtacgc atttctggcc aaagttcatc atgtcttttt
    12781 ttgtattctt cttgtttttc tgggtaaact ttcatgcaaa aagctttttt caccatgtca
    12841 gacacctgct ttcgtatgaa cttcctccaa ataacctgct tttggagtga caccaaacgc
    12901 atctgctaaa tgccacaggt tttcgtccgt gattgtttgc aagattggtc cttgcgcttt
    12961 gacgtacgta tagacttctg ctgccttttc tgctgtctcg atcaaaccaa agacttcatc
    13021 catgtctttt cctgacccgt agattccatg ttgtggccat aaaaccaatc gagtttcttt
    13081 cattttttca gctgttgctt ccccaatctc atttgttccc ggaaccatcc acggaatgat
    13141 ggaaattcct tctggaaaaa caactaagca ttccgtacac atttgccata gcgtacgact
    13201 aaaggaacgt tcgtctaatt catgcgtaaa actcatcgct aatagatgac tagcatggca
    13261 atgcataacg atccgattat ctgggtctac ttctaaacgg gcgatatgac tcatgaaatg
    13321 acttggcaac tcgcttgtag gaaggcattc gttttccagc ccccacaaaa gttccagtgt
    13381 tttgccatct tcagcaactc gaacgattcc aagattctcg gcaggttcgc tcgctacatt
    13441 tttaaaatat cttcctgaac cagttacaat aaaatagcat cctgctaaac ggctggcatc
    13501 aaaaatcatc gagatgcggc ggatcacttt ttcaggatcc aagaacggtg tgatctcttc
    13561 ttcttttagt aaataagaaa tattcccgcc atttctttca tcccatccca agcgataaag
    13621 attcgacgtc gtttcgatca tttctttcac ataaggcgct tgtaaaatat ctattttttt
    13681 caatatagcc acactcctat tttcttaatt ccaacacgtc tttttgatat tcctggactt
    13741 cttccagcca atccaaacca actggaactt gatttttgta acagaaataa ttccatacat
    13801 cggcatacgg gaaatctttc aattcttccg tataagccat tcttgaagta aagtcaaaag
    13861 ccagctccat ttctttcaat cgctcaactg gttccagcat agctttcagc agtgcttttt
    13921 gtgtatttct cgtaccgatg acccaagcag cgatacggtt gatcgttgca tcaaagaaat
    13981 ccagaccgat atttgttttt tccaacaatt cgttacggac taattcttta gcgatttcct
    14041 gcaattcatc atccatcaaa accacatggt ctgaatccca acgcactgga cgggaaacat
    14101 gcaacatgat tcctttgctg aataaagcta atgaagataa tttattggaa atcacttctg
    14161 ttggatgaaa atgtccagca tctagacaaa caagtttgtc acgagtcaac ccgtaaccca
    14221 tataaaattc atgggaaccg actgtataag cttcagcgcc taaaccaaat agtttgcttt
    14281 ctactgcatc ttgtacatac cgttcatcta actcttctgt aaaaatttca tcgagtgatt
    14341 ccattaaacg ttcccgtggg gtaaaacgat caatcggatt atctttcatc ccatcaggta
    14401 cccaaaagtt attgatactt gtttgcccag tttctttccc aaagtactcc gcgattttgc
    14461 ggcttctttt cccgtgttcg atccagtatt cacgaacttc tttatctgga tgagccaatg
    14521 taaagccgtc tttcatcatt ggatgcgaaa agaaagtcgg attgaagtca agaccgattt
    14581 cttctttttt tgcccactct acccattttt caaaatgttt cggctcaatt tcatttagat
    14641 cgacagattc ttctgtatcc aaatagatag catgaaggtt caatttgtgt ttgcccggta
    14701 tcaacgaata agccttctcc agatcttgtc taagctcttc aggtgttcgt gcggctcctg
    14761 gaaaattccc tgtcgccatg attccaccgg tcaattcgcc atccggtgtc aagaaacctt
    14821 tgacatcatc tccttgccag caatgcatag aaattttgat gtcctgtatc ttttctagta
    14881 cagcatctgt atctacaccg atagttgcat atttttcttt tgcttgttcg taatttcttt
    14941 caatcgtgtt catacgatta ctcctttgat tttattcacg taccattcca tcgtgttttg
    15001 cttattttca tctgggtaaa aagtttcaca tggaaacgac tgtttgatta catttcttgc
    15061 ttgttctata ttcgaaaact gacctgtact gagcatttgc atggcaacat tcccgattgc
    15121 tgtggcttca ctaggtcctg cttctacaat gattccggca taatctgctg tcaattgatt
    15181 cagtagacga ttattcgatc cgccccctac gatgtgcagc ttttccaatt tcgttccagt
    15241 taagtgttct actttgatca gctcttgtgc gtaacaaaga gcaagattat catacacaca
    15301 acgagcgata gctcccgatg agacagggac tggctgctct gtttcaaaac agtattgctg
    15361 aatctcttct atcatatttg ggggattcaa aaatcgaggg tcattgattt ccatcacttg
    15421 ctgcagcggt ttttcttttg cagcaagctc tgccaattcc gcataactgt actcgtactc
    15481 ttgcattcgg gcaacttcct ggatcaacca catccccatg atgtttttca aaaaacgcac
    15541 cgtgttatgg gcaccccatt cattcgtata attttcagaa aaagcattgc gagaaacatt
    15601 ggcgatcttt gtttcaatcc ctaaaagcga ccatgtccca ctgctgatat aaccccaacg
    15661 ttctccttga cctggcgttc ctaatacagc tgacgccgta tcatgacttg ctactgtgat
    15721 aaaggtcaca tctggcaaat catattttga aaattgttct ttttgcagat ctcctagtat
    15781 cgttcccggc tctgtcaatg gtggaaataa ttccttcgag acaccgattt tttcaagaag
    15841 ttctatttcc cattcttttg tccctgcatt cagcagctgc atagtagatg cattagtttt
    15901 ctccgtcacc atgttaccag taaagacata ccctaaataa tcaggtatca gtaacaattt
    15961 atgtgctttt gccaaatttt ctttttcttc agcaaacaat tggaacaagg tattgaaagg
    16021 ctggatctga atccctgtct gctcatacaa cttatctaga gaatagattt cagaaaactg
    16081 ttctactgct ccattggtac gcttgtcccg ataagcaatc ggatcattta tccgatcccc
    16141 ttgttcatca atcagacagt aatcgactgc ccatgtatcg atccccgcaa aaaggcgatc
    16201 aatccctgtc tgctttaatt tttcaaatcc aatcaaaatc tctttgataa gataatcgat
    16261 cttccagcga tcgtgaccgt ctttgttccg aaagccgttt ttgaaccggt gtacttcttc
    16321 caagatcaaa gaaccttttg catccctcgt acttttcatt agtcgtccgc ttgatgcacc
    16381 gatatcgatg gcaacaaatg ttggattcat ttctccacct cttttacttt attgttgatt
    16441 agcactttat taataaagcg ttatcattta ttgacaatgc tatcataata cagaatctta
    16501 tattttgctt cacactattt tggtatataa ttgcattatc ttgacaagtt gggagtgttt
    16561 acatggatta ctgggaaaaa gccaaccact catggtcgga agactcaaaa agagtgatct
    16621 tgacgcctac acaaagaagc aggaatttat ttttttatat ccaagaaatc ggttacttca
    16681 aagcaccaat cccttactat actgaacgag cacatttacc ttcttacttg ataaagttta
    16741 ctttgtctgg caaaggttcg cttacatata atgataatca gcaacagcta aaagctggag
    16801 acttattttt tattgactgc caagaatatc agcgttatca aacgatcagc gaagaaccat
    16861 gggaaatgga ttggatccat ttgaatggtc agaacgtcca atcattttat caggagttca
    16921 caaaagacgg ccataacacc ttccattcaa caggagatcc atcagaaaac aagatccacc
    16981 atttgatcac agaattgatc cacatgcaac aaggcacgaa tgcccgtacc gatttccagt
    17041 cctctgtagc tatccacgag ttgctgaatg aactgatttt gcaaaagtat cagctagatt
    17101 tcgaagaaga ggatattccc gcctacgttt acaaactgaa gcaatactta gacgaacatt
    17161 tcaaagaaac gattacactt gatcaattag aacaacaatt cttattgaat aaatatcagc
    17221 tgagcaaaga tttttccaaa tttatcggtt ctccaccaat cgattatttg atcagcaaaa
    17281 aactttctta cgctaaagac cttcttcggt atacagaatt tacggttcga atgatttccg
    17341 aagaaatagg gattgagaat tttgcttact ttagccgcct gttcaaagca aaaacaggac
    17401 tatcgccgag agactttcgt aaattcagct gattcatata atatgtacgt cattttttca
    17461 aaatattttg aaatatatag ataaaaataa aataaccgca gtttctgctt ctatgtaaac
    17521 aacagcagct actgcggtta ttttttagtg atcattctca ttgttcatct tcaaaatgtt
    17581 cataatgaac caactgtttc tgtgtatcac gtttcaaaaa ttctttaggc gttatgccga
    17641 actctctctt gaatgctttc agaaaatgcg gataagaaga aaaaccactt ttactatata
    17701 cagcactaga gctgcggtat tctcgcaaca agtgcttggc atagatcagc tttttcttca
    17761 ttacatactg atgaaacgta aatccagtgt actttttaaa ttctcgtgtg atatggtatt
    17821 tgctgacaaa aaactgcttt tccatttcct ctaaagaaag cggatgagat aaattttttg
    17881 agatatattg taacatttgt tccacacgct cattaggaac tacagcattc tgttcttgcg
    17941 cactctcttc tttttgtaca acttgattga gaaaaatcaa gtattctacc agagcttgtt
    18001 catattttat gtcttcaccg taaatttttt tatcaggagg atggtcgata aatgataatt
    18061 tttgcttcaa tacttcctgt tctatttgca atacacgact cctcttctct cccattggtt
    18121 gaaaacaagc cgatagatta gaccatggcg tggaacgact ttctagaaaa gaaggggtga
    18181 caaagatata gacacgctcg aatacatctg aagattggcg gacgatacga tgcaaatcat
    18241 tggaatggat gagcagcaca cttcctgctt tgatcgtaaa ttgtcgtccg ttcaaataaa
    18301 aagtcgcttc tccctttaat gtagcatgga tctcatagaa atcatgatga tgatagatcg
    18361 ttttattttg gctgacgaca tctttgacat gatagatttc atactgatca gaaaccatcg
    18421 tattgacttg ttcaccgctg attttctttt ggagcatgtg ccgcacctcc ttttgttcac
    18481 ttatgcacaa ttttattata ccacgttttt tgtaatcaaa aagagctgct tttatatact
    18541 cttataaaaa agaagagacg tggcatcttc tagccccgcc ccctccattc taaatattag
    18601 ttaaccaacg tcttctttac attattcaag aagacttctt ccataacgaa tcccatcatc
    18661 ttttgtttct tcaagaacca catacaggta cggacgattc ttcatgatga tttctttgat
    18721 ttttgtgtac tcgatcacac cgtctcccaa atttaccggc acgatttttt cctgttcgac
    18781 acgaaaatct ttcaaatgaa ccgcacatat cttttcacca aaacgttcaa aagcttcttc
    18841 taccaattgg acttgttctt gataatcact acttgtgatc aaattggtcg gatctaaaat
    18901 gattcctaga tagtccgaat ctactgcttg gatcagctgc tctacccttg ctaaagaata
    18961 caccggatga ttcaaacctg gttctatccc caccatcatt tggtattttt ctccagcttt
    19021 taccaagcga cggataacgg acacagcttc agcaaatgct tcatctgtga aattttcttc
    19081 cgtatattgg atttccggca aaacacatcc agtttccgaa gcaaccatcg acgcaccaaa
    19141 ggaagaagca tagcgaagat actgctcgaa ctttgtcagc aactcttgtc gtatcgttaa
    19201 atctggatga atcatattga tatagcaact taaaattcct acggtcactt cttgcttttc
    19261 taaaactcgt ttgacataat ttcccatccc agaattgatc tcatttgcgc cagaaggtat
    19321 ttcaggaaac gaaagaccta aggcgagctg cagtgtatga atcccttgtt cttttgtttt
    19381 ttctgcaaga tcttctatcg tttggacttc ggaaagatca tgtcctctaa ttgtaatgtt
    19441 caacataact attctcctaa ctcagcatat tgggtagcca catgacgatt tgcgggaagt
    19501 aagtaatcaa aaacaagacg actaagataa ccccataaaa aggaagaagc ggtttcagca
    19561 ttgcctctgc ttttacgcct cctacacttg caccgacata tagtcctgta cctactggcg
    19621 gcgtgatgga gcctacacac agattcatga ttgaaaacaa cccgtaatgt acaggatcaa
    19681 cccctacact tgtgacgatt ggtaaaaata ttggtgtaaa aatcaagata gcaggcgcca
    19741 catccataaa catacccacc agtaataaca caagattgac taataacaga ataataaatt
    19801 tattatccgt tacgcccata atcaacgaac taattgcctg aggaataccg gtaaacgcca
    19861 tggcgaaaga catcatggaa gaagtagcca ataagaacat gatcgtccct gacattgcta
    19921 ctgattgaaa caacatatta ggtatatctt ttagttttac cgttttatag taaaacatag
    19981 caatcaacag ggagtaaatg acagctatcg ctgaagcttc tactgctgta aatatccccg
    20041 tcaagatacc accgataatg atgacaatca atagaaggct aggaatcgcc tcttttatta
    20101 tttttccagc atgagccatc tgtccttttt cgacaacagg gtatttattc tttttagcaa
    20161 cgatgaatgc tactagcatg attcctagcg cccacaaagc gcctacgaga taacctccca
    20221 taaacaaagc agagatcgaa gcaccaccag caacaagtga atacatgata aaagcagtac
    20281 ttggaggaat gaccatacct gtcggagctg acgcgatatt gaccgctgtc gcaaacttcc
    20341 ggtcatatcc ttcttccact tgctgaggga tcaacactcc gccaatcgcg gtagaagcgg
    20401 caatggccga gctagagatg gaaccaaata aagcattccc taacacattc gtatgagcca
    20461 aagcccccgg cacgcgcccg acgaacaaca tcgcaaaatc cactagtttc ttagctatcc
    20521 ctccattatt catgataatg cccgagagaa cgaaaaacgg aatagcgatc agcgaaaaac
    20581 tattgatact tcccaccatt ttttgagctg aactaaaagc agaaacatca aacggtataa
    20641 ctaacaataa agtcatcatc gatgagaatg caatacttat agcaatcggc atccctaata
    20701 caagcaacac aacaaataca aaaaataaca ctaatccggc ttctattgcc attttattcc
    20761 tccattttta tattttttct tgaattgctg gcttgttgct taatgtatcg attagattga
    20821 tcaggctata gataatgatg agtacccccg atacaggtac tgacagataa acatatccca
    20881 taggaatact taaagaaggc gagatctgcc ccattgtcaa tgtgacagca ttccatccgc
    20941 catacaccat gatcatcgca gcaaatacca gaaataaact ttgaacaaca acttctaaaa
    21001 ttttactttt tttgccagtt aatttttcac ttaaaaccgt tacggctaaa tgtgcttttt
    21061 gtcccacaac atacgcggaa gacaacatgg caaaccagac aagaccgaat cgaacaagct
    21121 cttccgtgac cgtatttgga tttcctaaaa ccgttctgga aaatacttgc cataaaacaa
    21181 cagcgaccat cccaacaagc aaaacagctc ccaggatctc taataccttg tttaaaatca
    21241 ttttcatcac agctacgctc cttgctcttg gatcttttga taaaggtctt tgtaggtagg
    21301 atcgtttttg aatatatcat gaagtggggc aactgcctct cgaaacggtg tattatctac
    21361 atcattgaat tggactccat gttcttcttg tgctaaagct ttttcttctt caattgcttt
    21421 tgcaaaagct tcaatttcaa aaaccgttga ttctttcgct gcttcttcaa tggctttttc
    21481 ctgctcatca gttagctgtt ctttgatggc atcattcatg atgatgatat ctggcacacg
    21541 tgtatgctca tcataggaat agtatttagc cacaccacca tgtccagctg tatacaagac
    21601 aaattcattg ttttctgatc cgttgatcaa attggattga agagacgtgt acacttcatc
    21661 acttcccata ggtacgggcg aaccgcctaa aagctctacc attttgatag cggtttcgct
    21721 ttgcatcaca cggatcttct ttcccttcaa atcttccggc gtatggatcg gaccgtctac
    21781 catataaaag ctgcgctgac cagaatcata ataggtcaat ccggtaaatc ctaaattttt
    21841 ggtcgattgg tagattggct ccattatttc ttcattatcc atcaccttaa agaaatgttc
    21901 ttcatcattg aatagataag gaactgaaaa aatcgaataa tctttagaga aactttccaa
    21961 cgctgaggca cttacttttg tgaaatctac tgctcctgac tgtgtcaatt cgatcagttc
    22021 acgttctcca cccagttgtc catctgggaa atactggatc tgtacggaac catctgtttt
    22081 ttcttttact aaacgcccaa attcattcat tgcatcaatt actggctggc tgttgcttgc
    22141 ataagcaaag cgcaaggtaa ctatctcatt tacatctgca tctgcgacag ttgccacact
    22201 agaattattt ccgcagccgc ttagacctag cagacctagg ctaagcgaac ttgctgcaaa
    22261 cagcagtgat gtttttttca tatgggtttc ctttctttta acaataagag acgagcaatt
    22321 ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat tgctgtgatt ccgatattcc
    22381 gataaaaatc taaactgtcc cccattcctg tcccgattga aacgttttga acttctcctt
    22441 tttcatcgat ttgttcaagc aatccttgga tagcacgata agcgacttct ttatattctt
    22501 gtggtaaata acgtttatgg accgctttca aaataccgta agcaaatcct gcagttgcag
    22561 aagattctac gtaagaagaa ggatcatcca acaacgtatg ccataaaccg ctttcagact
    22621 gataagtttt taatgcacgc acttgtgctt caagtgtttc gatcaataat ttgcgcaatg
    22681 catcttcttt cgataattct aagatttcaa tgatctctgg aatagcaatc gtcaaccagc
    22741 agtttccacg agcccaaaat gcttcggcat agttatgatt tccttcaaac gtccagccgt
    22801 gataccacaa tcctgttcgt ttatccatca agtatttgat atggatcata aactggtaac
    22861 gagcttcttc taaatattct gggcgattca acagtttccc gatttttgcc aaaggaagca
    22921 ctgtcatcat caatgtatcg tcccacaact gattcttatt ttccggtcca tatgtggcat
    22981 gctgtaaccc gtcttcattt gtccgaggca tgtcattcat gacccattct gcccattgtt
    23041 ctaaataagg caaatagcgt gaatctttcg tgtcttcata caagtaagcc atagtcaata
    23101 gcggtgccat tgtattgacg tttttgggcg gagcgccttc ttccatacga tcctcaaacc
    23161 attcattgac cacttgataa gcgcgaggat tttttgtcat ctggtaattt ttataaatac
    23221 catatagacc gacaccttgt ggccaattcc acactgtcca gcttttgtca tctactttca
    23281 gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt gatcgttgtc aggttttcaa
    23341 cgagcaggtc aattttttct ttcactaatt ctttaggtac aaaaatctct tctttaataa
    23401 tcatctttac gccacctttt taaacgcttt cattttatat ccttaatata gcagtaatac
    23461 cgtccactat aaatatcgct ttttgaacaa aaactgtcta aaattgacct aaatgttaaa
    23521 taacaaaagt aactttcagt tacttggaaa ttaaaaaaag aacttggaga aacaacgttt
    23581 tccaagttct ttttcatcta ttttcttatc ttttttctac gctttctttt gcttatccgt
    23641 tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa tggttctttc ggattagctg
    23701 tcgtacattg gtcttcgtaa gctaattcag ccatacggtc tacggttgta tctagtgttt
    23761 cttgtgtgac tccttgagct tttagactca tgttgatgcc gacagatttt cctaattcat
    23821 aaacagccgt tgctaaagct tcgactaatt cctctgttgt ttcgcctttc agacctaaga
    23881 attttgcgat atctgcgtaa tctgtatctg cacggaaata gtcatattta gggaacatcg
    23941 catgtttttg tggatctttt gcattatagc ggataatatg cggtaaaaga atcgcatttg
    24001 tccgtccatg agggatcccg tattccccgc cgattttatg tgcgactgag tgacaaatac
    24061 ctaggaatgc gttagcaaac gccataccag ccattgtaga agcgttgtgc attttttcac
    24121 gtgattccat atcaggtgtt tttactgatt tttctaaata gtcaaagact aacttgattg
    24181 cttgtaggct caatccacgt gtataatctg aagccatgac tgaaacatag gattcgattg
    24241 catgtgttaa cacatccatc ccggtatctg ctgtgacaga tgctggaact gacatgacga
    24301 attgaggatc gacgattgcg acatctggcg tcaatgcata atctgctaac ggatatttca
    24361 catgtgtttc actgtcagtg atgaccgcaa atggcgttac ttcagaacca gtccctgatg
    24421 ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc gatcttatat gtccgtttac
    24481 ggatatccaa gaatttttgt tttgcaccaa agaaagatgt gtctgggtgt tcatagaaca
    24541 tccacattcc cttagctgcg tccattgcag aaccgcctcc aagagcgatt actgtatctg
    24601 gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt atttgtcgat gggttcggtt
    24661 caacatctga gaagacttcg atcttcacat catttttacg tcgtgccaat acttcacgaa
    24721 ctgtatcgca gtaaccgaat tggaccatac cagggtcaca aacgatcatg acacgttcaa
    24781 cgttttccat tttctctaag tacaatagag agttcttttc aaagaagatt tttggcggta
    24841 atttgaacca ttgcatgtta tttctccgtt tcgctacagt tttgacattg attaagttta
    24901 cagcagagac gttacgagaa acagagttct taccataaga accgcatccc aaagtcaatg
    24961 acggaatcat ttcgttatag atatcaccga tgccgccttc tgcagatggt gtattcacta
    25021 aaatacgaca agctttcatt cgaagaccga atttgacatg caactcttcg ttttctgaat
    25081 ggataactgc tgtatgtcct aatcctccaa gattcaacat accttcacac aaatcaaacg
    25141 catggtccgt gttgttagct ttgatcatgg caagaactgg agaaagtttt tctcttgata
    25201 gcggataatc tgggcctacg ccttccaatt cagcaatcag cattttcgtt ccttttggta
    25261 cactgattcc tgccaatttt gcgatttcca tcgcagaatg tccaacgatt gatggattga
    25321 ccgcatattt tccttcgttc atcactgcat cttctaattt ttgcaattca tctggtttta
    25381 cgatataaac ttggtgtgct tggaattctg cttttactgc tgcatagatt tctttatcca
    25441 cgatcacagc ttgttcagaa gcacaaatca ttccattatc aaatgttttc gaaacgatca
    25501 aatcgttcac tgcacgttta atttttgctg ttttttcaat ataagctggt acgttacctg
    25561 gacctacacc taaagctggt tttcctgtag agtaagcaga cttgaccatt ccagctccac
    25621 ctgtcgctaa gacgatcgcg atacctggat ggttcattag catagaagtc gcttcgatcg
    25681 aaggatgttc gatccattgg atacaatttt caggagctcc ggcagcgata gcagcatcac
    25741 gtaccactcg tgctgcttct gcagaagatt tttgggcact tggatggaaa gcaaaaacga
    25801 ttggatttct tgttttcaac gcgatcattg atttgaaaat cgttgtggaa gtcggattcg
    25861 ttgttggagt tactccgcaa accacaccga ctggttcagc aatttcaatc aacccttttt
    25921 ggacatcttc attgatcaca ccaactgttt tatcatgttt gatattatgc caaatatatt
    25981 cggacgcata catatttttg attgctttat cttcgtaaac accgcggcct gtttcctcta
    26041 cagccatctt cgctaatggc atatgttgat ccaaagctgc cattgccatt tcatgcacga
    26101 tatggtctac tttttcttga tcaaagcttt ccatttcttt caatgctgca tttgcttttt
    26161 ctgccaaatc atcaatcatt gcttgtacgt caattgtctt cgttttttct ttttctatta
    26221 ctttagccat cattatcctc ctaatgggtc atggttattt gttaaataat tcacatgtat
    26281 atcatacaat atttattttt ctatgtaaac actttttttg tgattttttt cacagttatt
    26341 tttaaatata aaattgcttt atttacaggt gtttgtatag aaaaatatat aaagttattt
    26401 attattattt atatgacaca cccgattatt caatattgag caatatatcc catgctctta
    26461 tctctttctt ttttgaataa aaaatgaggt aggacatctt tctgccctac ctcgtcttta
    26521 aataagcttt gatacttatt tttcttcctt ctcttctgtc tcttccagcg tttctgttgt
    26581 ttcagttttt acttcaactg tctcagctgg ttcttctgtt gtttcaacag tagtcgttgc
    26641 atcatttgtt acgacagtcc ctggtttgac cgttttgatt gctgcacgat cgtactcaag
    26701 gaagatccct tcgcagtcta aaacaactgt ccgtttgtca ttgtcgattt ctgaaacaac
    26761 tccatgtaat ccgccgatcg ttacgactga atcacctggt ttcatgctat ctaacaagtt
    26821 ttgacgttct tgctgttgtt tcttttgcga acgtgacata aaaaaccaca ttgctaatag
    26881 cgcgataaac atcacaagca ttggtaatgt tcccattttt ttcacctcac taattctgta
    26941 tctacaaaaa acactttagc agaaaaatgc tgataacact agttttttac ttaattttta
    27001 aaagattttc ctttagaagc tttttgcgtt ttctttattg aacccgtatt cttcaaaaaa
    27061 ggcttctcta aattcaagca aattatcatc catgattgct tgacgtactt gtttcattag
    27121 attcaacaag aagtataaat tgtggtaaga agtcaaacga atcccaaatg tttcatcaca
    27181 tttgatcaaa tggcggatat aggcacgtgt atagttttta catgtataac aatcacattt
    27241 ctcatcaatt ggacggaaat catgggcata tttcgcattt ttcactacta gacggccttg
    27301 agaagtcata catgtgccat ttcgagcgat tcgagtaggc aagacacagt caaacatgtc
    27361 tactccacgg ataacaccat cgatcaatga atcagcagca ccgacaccca tcaagtaacg
    27421 tggtttgttt tcggggatca acggtgtagt gaattctaat acacgattca tttctgattt
    27481 aggttccccg acagataacc cgccgattga gtaaccaggg aaatccatcc cgactagatc
    27541 tttcgcgctt tgacgacgaa gatcttcaaa ccctgctcct tggatgattc caaataatcc
    27601 ttgacggtct ggattagcat gagctttcaa tcctcgttct gcccatcgag acgtacgttc
    27661 tactgatttt ttcacgtagt cgtaactttc atcgaatggt ggacattcgt caaaactcat
    27721 catgatatca gagcccaatt tattttggat gttgattgct ttttctggtg ataaaaacat
    27781 acgcgaaccg ttcaagtgat ttttgaaatg cacgccttct tcttcgatat tccgcatatc
    27841 acttaatgaa aaaacttgaa agccgcctga atctgttaaa atcggttgat cccagttcat
    27901 aaatttatgc agtccgccag cttcttctac taagtcttca ccagggcgta gccataaatg
    27961 atatgtatta cttaaaataa cgccagcacc catttctttc aactcttctg gggacattgt
    28021 ttttactgta gctaatgttc ctactggcat gaacattggt gtggggaaag ttccgtgtgg
    28081 cgtgatcaat tcccctaaac gcgcgccagt atgtttttct ttcttgatta aacgatagcg
    28141 aatggcaggt tgcgtcattc atttccctac tttctatata aaatatcttg acaactccca
    28201 ccatcataat cgtttcaagt gctttttgca agttccctcg gcttccttct actttattta
    28261 atattctttt ttttatcctt tttttatgaa taacaaaaaa aagaaaaaca ttatgacaga
    28321 aatatttttt gatacatggt atattggtgt tattaaagaa aaggagggaa aaatcatgca
    28381 atcttctgca gaattaaaac gccaagcaaa agagagtcta aaaggacgtt ggggacaagc
    28441 tgttttacta aatctaatcc ctacattgat tggtattgct actattcttc ttattatgat
    28501 tcctattatc ttgtttgcca ccaccatata tgatggatca gcgagcgtct cagaagcgac
    28561 aacttcagga ggcggtagtt caggaggcgg tggcggaatt atttccagca tcattagcgc
    28621 tttgttcatg agcggtattt cttggactta tcttgatatt ttacgtggac aaaaagatca
    28681 gatcgaacca ttcaaagatg catttcgtgg attcaaaggt ttgtttatcg gcggagtgat
    28741 cagcttagct gtgctgatct cgatctttac aacattatgg ggtttgctat tagttattcc
    28801 tggaatcatt aaaagctatt cttattctca aagctatttt atttactacg atatcgtcca
    28861 agaaacagga caaacaccta aagtactgga tacgatcact gcaagtcgaa aattgatgga
    28921 tggttataaa ggacaacttt tctggttaga tctttcattt atcggctggc atatcttagc
    28981 attagcaact gctggtattg gttatctatg gctcagccca tacatttctg caacaaaagc
    29041 agcattttat gaacaattgc caaaagatat ttaaaagaga gtgtgacttg tgaccgaggg
    29101 ggtggcagga gaacaccgtt tatcgagagg cagtaaaaga aacattcagc ccattagcaa
    29161 aaaggaggtg tgacaacatt catgtcacac ctcctttttg tatagttcca gtaaaaagaa
    29221 aatcaactta gaaacagtta atcattcctc ttcttctgct tccggtaatc gatggaactt
    29281 gatcagttca ttcaccgcga ttggtacgat agataaaatc cctaccaaga accagtgatt
    29341 caaactgatg tgtactaggc cgaatagttc ttgcgtaaat ggcagcaaag caacagccgt
    29401 tgtaatagct aatgccagta acgccatttc taccaaagct ttattggtcg ctaaattgac
    29461 tttgaagatc gattggctgc tgcgtacatt gaacacatgc agaatcgatg aataagctaa
    29521 gatcaagaat gcaacagttt gaccgacatg ctgacttgcg tctacccatg ggctgatagt
    29581 atcgacataa ctacctaaat aataaccaaa caaggttacg atggtgaaga cccctgcatt
    29641 tatggcaatt ttttgcaata aacctcgagc aaagatccct tcatttttcg gaatgggtgg
    29701 ctgatccatg atgccgtgtt ccgctggctc tcttcctaaa gcaaatcctg gaagtccatc
    29761 agcaactaca ttgacaaaca gtagttgcac agctgtaaat ggtgctcccc atcctaacag
    29821 catcgcaatc aaaacaataa agatctccga aatattacag cttagcaaaa agttgatagc
    29881 tttacgaata ttctgataaa ccgaacgtcc ttgactgaca gcgtctacga tcgtcgcaaa
    29941 gttgtcatcc gtcaaaatca tatctgcggc actttgcgcc acatctgttc ctgtgatccc
    30001 catggcacac ccaacgttac ttgctttcaa cgctggtgca tcattcacac catccccagt
    30061 catcgctacg actgcacccg tacgttgcca agatttgacg atccggatct tgtcttctgg
    30121 cgttacccga gcatagacag agagagcttt gacacgcgca tctaattctt cgtctgataa
    30181 ttgatgcagt tctgatcctg aaagtgcttc tgacggatca ttcaagatac ctaattcttt
    30241 cgctatggca ctggcagtca ccacatgatc tccagtgatc atgactgttt tgatgccagc
    30301 ctttttcgct cgcttgattg ctcctttact ttctggacgt ggtggatcga tcattccgat
    30361 cagaccaatc aaacgtaagt ctttttccag tgcttcagaa gaaatatctt gcggttcttc
    30421 gtcataagta gcataaccaa cagcaatcac tctcagtgcc cgtttaccaa agcgatcatt
    30481 aacgatcgcc gcttgttcta catcgcctga actaaagcgc ggagcaagaa catcgaatgc
    30541 tcctttggtg attgagatgt atttttcgcc catttggtga actgttgtca tcatttttcg
    30601 ttcagaatca aaaggaagct cgtttactcg tggatatttt tcttctaatt ctgcctttgt
    30661 atgataattc tcttcaacag cccttacgat ggctacttct gttggattac ctttgataac
    30721 aaggtcgtca tcttcttgct cgaccgtcac atctgtacat agtgcagcca ttttcaatat
    30781 ttccatcgcg ctgttcgtca ttgaatcttc gatattcgtt acttcatctt ctttgctcca
    30841 tacccggcgg acgcgcattt cattttgtgt cagcgttcct gttttgtctg agcagataac
    30901 attagcggtt cctagcgttt caactgctgg caactgtcgg atgatcgcat gtttcctcgc
    30961 catcttctgc acaccgtatg caagtgtcaa cgtaacaata acagtcaagg tttccggtac
    31021 agctgcaaca gccaaggaaa tcgaagtcat gaacatctca aggagtggtt ctccttgcag
    31081 ttcaccaatc acaaatacta aagccgcagc cgctaaagcg atcatactga ttcgtttgcc
    31141 tagctgattc aatcggacct gtaaaggtgt tttttgcata gtattttcgt tcaaaagatc
    31201 cgcgatctta cccatttctg tagccatgcc aatggcagtg acgatcgctt tcccacgacc
    31261 tgcagcaacc gtacatcctt taaataccat attaagttgg tcacctaatg actcatcttc
    31321 ttcagcaata aatgctgcat ctttttcaac tgcttcactt tctccagtca acgccgattc
    31381 ttctacacgt aattgtgatg cttcgatcaa acgggcatcg gcagaaatag catcaccgga
    31441 ctctaaaaca agaatatcac ccactaccag ttgctctgac tcaacagtgg tcactttgcc
    31501 gtctcgtatg acagttgccg tttgtttatt catatcttgc aaggattcca gtgctttttc
    31561 tgcgtttcct tcttggacaa tcgccaatac cgagttgatc acaacgatag cgatgatcag
    31621 cagtccttca aataaatccc catgttctgt agcaaatgcg gtataaaagg aaatcaccgc
    31681 tgctacaaga aggatgatcg ttgtaaaatc ggacaagctt cgaaagaatt ttttgatcat
    31741 cgattctttc ggcgcctcat cgaatttatt caatccctgc ttttttcgcc gttcatccac
    31801 ttcttgtgaa gacaacccag tatctggatc tgcttctgtt accttcttta cttcttctat
    31861 tgtctgcttg tattcttcca tcctattcct cctttaaaat cttcgtacaa aaaaaggtac
    31921 ttattttact ataacatcct ttttttattt acttcaaata aagggcttat cgattatttg
    31981 ttaagaaagc gtaaaattta aagtgcttat atttgcaaac gctaccaaag gatgctatta
    32041 tatagatgta aggaaggagg ataccatcaa atagacagaa gaatttctcg atagaacagt
    32101 gtaagcaaac aaagttgtta gaagtacagg tttcacccag acatgcgtgt agagtacaaa
    32161 cctataaata gtgaatgcgt atggatgttt ggatcacgaa gtatccgaaa caagaagtaa
    32221 tgcgatacgt aacaattgaa taacgtaaac gtctgaaaat tgaataggaa caagagatgt
    32281 ttcaaatgtt gaagatgtaa cgaaaacaaa atttttgttt aacatgaaga aattcaacac
    32341 cgtgctttgc atgtatgatg gaaagttaca atcatttctg tttatttgaa tccttccgag
    32401 gtacaagaaa gccgagctta tcgagagctc ggctttttct ttgtcataaa atctttgtaa
    32461 agcttttttg ttattcaaaa ctagcggctt ttcataacct tatttcacga acatcgcatc
    32521 tccaaaacta aagaaacgat agcgctcttc aatcgcatgc tggtaagcag ataaagtcaa
    32581 ttctcttcct gcaaaggcac taactaacat gactaaagtc gattttggca aatggaaatt
    32641 agttgagaaa gcttctacca ctttgaattc atagccaggc gtaatgaaaa tatcagtcca
    32701 tccgctgtct gctttgattt ctccattaaa tttcgttcca atcgtttcta atgttcgaat
    32761 tgaagttgtt ccgacagcca cgattttccc gccattttta cgtacttggt tcaatcgctc
    32821 agccgcttct tccgtcaaac ggtagaattc actatgcata tggtgttctt caattttatc
    32881 tacgctgaca ggtcggaatg tacctaaccc tacatgtagt gtcagataga ctaattcaac
    32941 accctttgct tcgatctcat caagtaattc tttcgtaaaa tgcaatccag ctgttggcgc
    33001 tgcagcagaa ccattttctt tcgcatagac agtttgatag cgatctgggt cttccagacg
    33061 ttctttgata tatggaggca atggcatttc tcccaatgat tcaagaatct ctagaaaaat
    33121 tccttcatac tcaaagtcaa tgatccgtcc gccgtgttcc aattcttctt tcactactgc
    33181 ttgcaatctg ccatcaccaa aagagattct tgtcccaact ttggcacgtt tcgcaggttt
    33241 gatcaatgtc tcccacgtat ctccttgtgt attcgttaga agaaggactt ctaaatgcgc
    33301 accagtatct ggtttttctc catataaacg agcaggcaga actcgtgtat tattcataac
    33361 tagcgcatca ccaggctgta actcttcaat gatttcatgg aaatgtttat cttccatctc
    33421 tccagttttc cgatccaaaa caagtaaacg agaatgatcg cggtctttta atggtgtttg
    33481 tgcgattaat tcttccggca agtcaaaatc aaaatcttct gttgttaaca tatcgtcact
    33541 cactttctat catgtgcttc atcattctat catgtttttc gttctgttgg caaaatcagc
    33601 actttttcac ttctattttt tgttttccta aataaaaaaa ctaccctatt tgtatttttt
    33661 cattgtttta ggcatactag taatagatag ttcttcattg aaaatagaaa aagaggtggc
    33721 accatgattg aatttcaaaa tgtgtcaaaa atttataaag gtggaaaagt cgctgtagag
    33781 gacgtcaatc tttcatttga aaagggtgag ttcatttgtt ttatcggtac gagcggaagc
    33841 ggaaaaacga cgacgatgcg gatgatcaac cgaatgactg atccaacaca agggaaaatt
    33901 ttgattgatg gaaaatccat ccaagaaatt gatccagtgg aattgcgacg tcagatcggc
    33961 tatgttatcc aaagtattgg attgatgcct cacatgacga tccgtgaaaa tatcactctt
    34021 gtccaaaaat tgttgaaagt cagtcaagaa gaacggaata aaacagctga aaaaatgatt
    34081 gatcttgtgg aattgccccg tgagatgtta gatcgctacc cacatgaatt atctggtggt
    34141 cagcagcagc gtattggagt cgttcgtgcc cttgcagcga atcaagatat catcttgatg
    34201 gacgagccat tcggtgcgct tgatccaatc actcgtgatt ccttgcaaga ccttgtcaaa
    34261 gatttacaag agcgtttggg aaaaacgatc gtatttgtaa cccatgacat ggatgaagcg
    34321 agtgaactac tcaccactta gctaaacctg acggttctta acgcttgaag tgggagcttc
    34381 ttgggaatag agcatacttg atagcgtatt tctttactaa cagcggtgtc tcttatttac
    34441 caagctatcc ccgtagttcc tacggttctt gatgattgga tctaagccaa tcccatagaa
    34501 tgtagtctta gaccttcggt cagaatattg atactagcat tgatatcacg atcatgatga
    34561 gtatgacaaa caggacaagt ccattctcga atttcaagag atttcttgcc atctttatgt
    34621 ccgcactctg aacaaatctg actagacgga aaccatttat ctactttgat gatttttcgt
    34681 ccataccagt cagccttgta ttgtaattta gtcacaaact ttgaccaaga aacatcagaa
    34741 atacttttag ctaatttatg atttcgcaac ataccttttg tgtttaagtc ttcaatacag
    34801 ataatatcgt gatttttgat catttctgta ctcaacttat tcagaaaatc agtacgttga
    34861 ttcattactt tttcatgcaa tctagctact ttgcgttttt gtttttgata atttttagct
    34921 tcaaatagat tggtaccttt atttttagcc aataaagcac gcctagacaa tttgcgttgt
    34981 tcacgcttta gtttcttttc cattttggac gtgaatttat tattatcaat tttttgtcca
    35041 tcagaaagaa tcgcaaagtc cataatacct aagtcaatac caattgtgga attagtttta
    35101 ggtaattcga taatttcttc tttacacaac aaagaaatat agtatttacc gctagaatga
    35161 cgtgatattg tagcagattt gataatccct tttggttgtc tatgaagctt aattcttact
    35221 aatgacttca atttaggaac tttgatgaat ttactatcaa tcaaagcaac tgtaccattt
    35281 tgattattcg ttgtataact ctgaacagga tttttcttac ttttgaaacg tggaaatcca
    35341 acggatttat cccgaaaaaa attcttgtat gctttatcta aattaagttg ggcattggct
    35401 aaagcaaggc tatcaatttc tttcaaaaac ggaaattctt tcttgtattt agctggtgtc
    35461 ggaaaagtca tttttttaga agaatcattt ttaacttctt catacgcctt ttttcggtca
    35521 tcaagcatta gattgtagac cttacggaca caaccaaaag atttggcaaa gaagatttct
    35581 tgttcctctg ttggatagat tctgaatttg tatgctttta gttgttccat aaagttcacc
    35641 tactttctat ttatgattgt ttccttgatt ttggatatac ttcttaatca catcaattgg
    35701 tgcgccacca gttgtcagaa gacaaaaact tttagaccag aacatttctt tccaaagaaa
    35761 ttgtttgact cttggaaagt cacgttttat caatcttgag ctggcacttt tataggcatt
    35821 gatgaatttt gtcaattctg ttttaggttg agctttgaac ataatatgaa cgtggtcttt
    35881 atcatgattc cactccacta aagtaatgtg atacgattct gaaattcttt caaaagtagt
    35941 tttggcataa tcagatattt catcatcaat cacttgtcta cgatatttcg ttactaaaac
    36001 aaggtggtaa taaagaagaa atactgaatg attatttgta tctaattcca tgttatatca
    36061 actcatttct tgtatagact gattgtaaca tggaacaaaa taaaaaggca atacgcctta
    36121 gttgtcggtc ttcgagttac caacggtaac cctcataccg aacggcattc atcacccacc
    36181 tatagaggat gggcgacttc tgcctaattc tgttaaacta gcaagccgta tcgccatcat
    36241 gagtgaaggc cgtgtgatcc aatttgatac accagaaaat attttacgtc atcctgcaaa
    36301 cgatttcgta gaagagttga ttggagaaga tcgcctacta caagccaagc caaatgcgac
    36361 aagagtcggc gaagttatgc taaatacagc aatcacgatt acaccggaaa aatcattaca
    36421 agaagcaatc aaactgatga gggaaaaacg tgtagatacg ttgttagtta ccgataattc
    36481 gaatgtatta aaaggattta tcgatgtaga aacgttgaat aaaaaacgag gaaaagtatc
    36541 cagtgtcggc gacattttga ataaacatgt tttttacgta aaagaatccg cctatcttcg
    36601 tgataccttg caacgcattt taaaacgcgg gctgaaatac gtgccggtcg ttgatgaaca
    36661 gaacaaagtc gtaggtatcc tgactcgtgc atcattagtg gatatcgtct acgatgtgat
    36721 ttggggagaa gatcccgcaa ctactgatgt tgcagagtca gctactcctg aagaatctaa
    36781 ggaggtctga cctatgcagc agttttttca tcagcacggt tcagaaattc tctcaaaagg
    36841 ttttgaacat ctttatattt caggacttgc tttgatcatt ggtatcatca ttgctgtacc
    36901 gcttggtgtc ttactgacac gttttccaaa agctgccacg attgtgattg gcttaaccag
    36961 tgccttgcag acaatccctt ctcttgcttt attggcattg atgatcccgc tatttggcgt
    37021 aggtaaatta cctgcgatta tcgcactctt tatttattca ttattgccga ttttacgtaa
    37081 tacgtacatt gggatgaaaa atgtcgattg gaattatcgg gatgtggcaa aaggcatggg
    37141 gatgactgag ttccaatcca ttttttccgt tgaattgccc attgctatgc caacgatcat
    37201 ggctggtatt cgtttagctg ccgtttacgt gattgcatgg gctacacttg cctcttatat
    37261 cggcgctggt ggtttaggcg atttgatttt tagtggactg aataattacc aaccagatct
    37321 cattttggca ggtacgatcc ctgtaacaat tttagcacta ttggctgatt ggcttctagg
    37381 tctcttggaa caccggctga caccgatcgc acttagagag gagaatgaag aatgaaacgt
    37441 atactaaaaa tgaccatggt tcttttctct cttcttatac ttgcgagttg ttccttccct
    37501 ggtcttgcca gcaatacaga tgaagacacc atttcgatca caggtggaat cacttcagaa
    37561 gcacagatct tagcaagtct agttgccgga atggttgaac attatacaga taaaaatacc
    37621 gcaatcatca acaatctggc tactacaact atcaaccatc aagcgatgat gaacggagat
    37681 gcttcaattt cagcggctcg ctacacagga acagatttaa cgactacatt gaaccttcct
    37741 cctgaaaaag atccgaaaaa agcgtttgct accgtgaaag atgaatttga gaaaagatac
    37801 ggtcaaacgt ggttcccgtc ctatggtttt gaaaatacgt atgtctttct tgttcgaaaa
    37861 gacaccgcac aaaaatacca tctatctaaa gtcagcgatc tgaaaaatgt agcggatgaa
    37921 ttagtagctg gtgtagatac ttcttggatc aatcgtaaag gagacggtta tgacggcttc
    37981 caagaaactt atggcttctc cttcaattcg atattaccta tgcagattgg acttgtttat
    38041 gacgcagtcg aagcaggaaa aatggatatt gttcttggct actctacgga tggacggatc
    38101 gccagttatg atctagtcat gttggaagat gatcgtcaat tcttcccacc atatgatgct
    38161 gctccagttg tagacaacaa gatattagct gatacgcctc atttaaaaga agccttaaat
    38221 aagttaggtc ataccatcag tacggaaaaa atgcaggaat tgaactacga agcagataat
    38281 aatctagtgg aaccatcagt agtagcacaa cgtttcttac aagaaaacca ctattttgaa
    38341 gggaagtgaa gcaattggat aaaatgaatg tctttcaaca atttttctat tatttccagg
    38401 aaaatggctc ctatatcttt gcacaattta tccgtcactt cctgatttct atctatggtg
    38461 tcctctttgc agcagttgtc gggattccgg ttgggatcat gatttctagg agacgtaaat
    38521 tagcaaactg ggtgattcgt ttggcgaata tcatccagac gatcccttct ctagccatga
    38581 tctccatttt aatcattgga ttaggcctgg gagtcaatgt cgtgattgtt actgtatttt
    38641 tgtattccct cttaccgatc atcaagaata cgtataccgg gatgatccaa gtagacaaaa
    38701 acatccttga tgtcggcaaa ggcatgggga tgactgctag acaacgacta ttcatggtag
    38761 agttaccact ctctgtttct gttatcatgg caggcataag aaacgcgtta gttgttgcca
    38821 tcgggataac cgcgatcggt gcattcgtcg gtgctggtgg tcttggagat atcattatcc
    38881 gaggaaccaa tgcaacagag ggcacatcga ttattttagc aggtgcattg ccaactgcat
    38941 taatggcgat catcacggac tggcttttag gtattttgga aagacggcta gatccagcca
    39001 gccgtacatc acgctgatgt tattgtaaaa aacaaaagct tctgcgacca tttgttggtc
    39061 gcaaaggctt ttttagttct gtcattttta aggcaataat ccactctgat tcttccctcc
    39121 ttatgggcaa agagttgctt ttcttagcta tttatccatt ccgtatactc caacttcata
    39181 ctgccaacgg gttcatatct gcatccaccg actaagtttt tttcttatcc gctaaggcat
    39241 tttgccaatc cattttccca tcactcgtca tggaattcat accacatgat ctctaaatac
    39301 tcgtctgctt gttctttgaa ttgggcatca attcttacat cctttggcgt ggctttcatt
    39361 taagcctttt ccatcagtcc tcgattgttc gatgccttca gctgcatcat caaaactggt
    39421 gactgccgta tcggtaactt cgatcatctt ttatacaatc gggaacttgc catcctcatc
    39481 cttggctaaa aacatatcta gccattccag cttctcgcca tgggaggttt gcggttccta
    39541 aaaccggtac acgattctta tggactgcat ccgtcgcaac tagacttggc gatacgatga
    39601 tccttcacca gaagagcacc cccgtaaacc aattgatcga tatattgcca ataagaaaag
    39661 acattggcgt aaaagatatc gatccctcgt gatgcattgc cgttgatact gctattcatg
    39721 gccgaaagcg ccacgacttt aacttcttcc gtttgagtga cattcatgtt cggcaatgtc
    39781 gctttggata cccgctctgc taaaggaacg acactggtat tgtatttcgt atctgtatct
    39841 tttccaaaag accatgccaa cgaatcctct gggaaccaat aagacgactc cggttgattg
    39901 tccattgctt ttttcaacat acttttggct gtttctcgat aaacgacatt tccaattgtc
    39961 ttcgcctcac tttggcgata atcgatggtc gttgccactg ccaacactaa caacgatgct
    40021 ccaattttac tgattttcct tgtcacactt cttcctcctt gttttacata aacccgtcaa
    40081 cattccattc ctaaactcaa acaatgaccc ttcaaaggtg gagatatagg aacaaattaa
    40141 agaaatcatg caataaatcc aagaaggttg gttttagaac aaaaaaagag tctccttttt
    40201 ggggagactc tttgttaact atctaaaaag gttggctctt ctcacttcac tcataatgtg
    40261 tccaggcaga ataaatttct acaactttat tttcctcatc aactttgtaa accactcgat
    40321 gctgtctatt cagcctgcgt gagtatctgc cttcatgtgt aggtctcagc ttttcaaaag
    40381 actgcgtggg catatatgga tcttccttca atgtctgaat cacttcttca aattgtttct
    40441 ttaaattcgt ttgcttgatt tttcttaaat ccactttcgc agagtttttg attgcgactg
    40501 tatagttact catcaaagag cttcccaatc aatatcatcg atattcgtaa agctactgtc
    40561 gtctttttcg cggtctcgta ctttatctaa agtacctgtt tgctcaagaa aaagggtttc
    40621 ttgaatcgct ctccagtctt ccagcccaat caatacagca ttgtttccac tacgatcact
    40681 aataatctcg atttcagtat gattttcatt tacctctttc aataactgat aaaaattttt
    40741 acgagcagtg ctaggattca tcgttgtatt agccattgtc tctcctcctt tttctgatat
    40801 aattatagcg tacttgatta agtacgtcaa aaaaatacaa taaagcactt aaattcagca
    40861 aacttttcct cttttttaca tagagaaaaa gacccctttt tagggaggat ctttgtccac
    40921 gctcacttct tactccttta cgactagcgg tagccaaacg taaagtaatt ggtaacaacg
    40981 tatcatcttt agcaaaggtc gaatccaaag cctgtgagtc tatgcaacaa caccaataca
    41041 cgaaggttcc tcgttgcagt gtgacttgat tgatatcttg aataacctga ggatacgcac
    41101 gaaagatttg taacggttat tcaaaactga ttttggccgt caagtcttct actgcctcaa
    41161 aaatgattgc ctctttcgtc tcctgtcatt catgttcact atcgatttga tacttgctta
    41221 gaccgttggg tttgtgaatc accaattaaa aatggcactg agttatggta acaaaaaatg
    41281 gtcacttgac gaggctgaga ttcctactta tgaagacaca tggcacttaa gagtcgaggt
    41341 aaaggaagaa gctgaaaata taggaggctg tacattattt ggcaaaggat tcggaaatta
    41401 tgatcaagac atcaaaatca aaatgtttta cagctaaata ggaagaataa taacatttca
    41461 tacgctgttc ccatctttaa tggcttatag agggcacctt tagctgagaa taagacaaaa
    41521 cagaccctct agcttcaatt aagagctaga gggtctgttt ttgattttaa aacttggttt
    41581 atttcttcaa atggtagcta taggtgctga caacgatatt ttcacgccaa gacaatcgca
    41641 atcttaaccg caaacttgtt tggttatgga gaatattctg ccagcgtctg tttggtacaa
    41701 attgaggaat caacacagta gttgtatagt tttgtttcgc tgcattttta cttacgatat
    41761 ctacataacg gataattgga ttttcaatcg agcgataaga agagtggacg attgaaaaac
    41821 ggacatctgg gaaatgcttt ttaaattctg cttcgatttc tttttctttt tcgacatctt
    41881 cgtctagcga aacatgcatc gcaacgacat aatcaccgat cgaacgtgcg taattcaacg
    41941 caccgatatt tacccgtgtc acattcccga ctaatacaag aacggtattc ccgtcataat
    42001 catgtagttt tgcatcattt tctaaacgaa gctgttctgc tactttttta taatgatcat
    42061 ggatcttata aaaaatgaac ataacgattg gcataatgat gaagaaaggc caaatgtccc
    42121 ctaaccgata gacaaacaaa atcgcaatga ttgcatatga gatgaacgca cccgtaatat
    42181 tggcaatcga tttgcttaac catctttttc cttctttttt ccatttgacg accatccctg
    42241 tttgagataa agcaaatgga atgaacacgc cgatagagta taacggaatc aagcgttctg
    42301 ttgagccatg gaaaataaac agcaatacga tcgaacctag cgctagtgtg ataatcccat
    42361 tggaatagcc tagtcgatcc ccacgatctt gatacatgtg aggcatgaat ttatcttttg
    42421 ccagattgta tgcaagaact ggaaaagcag aaaaaccggt gttagcagca actgctaaaa
    42481 tcagcgctgt agcaaattga aggacgtaat acaaaatacc atgtccaaag acagctttac
    42541 caatttgaga caaaacagtg acttcttttt caggaacgat cccataccaa tagttgataa
    42601 atgtgatccc cacgaagaag aaacctaaga tcaatgccat catcgtcaat gtagcagcgg
    42661 cattttttgc tcttggcttc ttaaagaatg gtacagcatt actaatcgcc tccaccccag
    42721 ttaaagagga agaaccagaa gaaaatgctc gtaaaatcaa agcgatagaa acacccggca
    42781 cagccgctcc tggaagtgca gtagcttgca atggcacagc acctgtcacg atattatata
    42841 aaccaacaac gatcagtaac gagatcacaa ggataaatgt gtacaccgga aataatagga
    42901 acgatgcaga ttccctcagc ccacgaagat tcagcatcat cagtagcaaa acgattgtca
    42961 cagaaatagc gacttgatga ccgtagagtg ctggcaccgc tgatgtgatc gcttcagcac
    43021 ctgctgagac agatactgct actgtcaaca tgtagtcgat caataaagag ccaccggaaa
    43081 tcaagccagc attctttcca agattctcac tgctgacgac atatgctccc ccgccatgag
    43141 gataagcatg gatgatttga cgatatgata gcgttaatga aatcaataaa ataataacaa
    43201 aagcggcaat gggtagcgaa taccagatgg ctgcagcaga caaagcgact aaaacgacaa
    43261 cgatttgttc cgtaccatat gcaatagatg ataacgcatc agacgacaat aatgccaaag
    43321 cagcaaatcg ggttaatttg tgctcatcat tttcagcaga ttttaaaggc ttacctacta
    43381 acaacctttt taagtaatcc aatttctaaa ctcctttaca cacaatttta cttcaagttt
    43441 tatagtcgaa aatttatagt aaacaagatg ttatagtacg ctcactgacc ttaaaagtca
    43501 ataaacttta agaaaataat tggaaaaaaa cagaaaaata tcaagcgctt tttcaaacgc
    43561 tttaatacag caaaaatcta acattttttt atgggctttt tctttgaatt ctataataaa
    43621 aggatagaac ttgacttaga aaatcaagtt ctatcctttt tgatatctta acttttggaa
    43681 gagagatgta aggctgcttt ctacccatca ttcttactac tcgaagcata gcgcagttgt
    43741 cacaacctct tgataaacgg tgtttaaaag cttgcatctg cggtaataag cccaaccaaa
    43801 ttaaaaacat aagaagctgt ttttatttgg tcgttcttat tacttgatgc agagcagctt
    43861 ttttacaacc tcttggtaca cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
    43921 acggcaaaat atgaggaact atttttccat ttttttctct aatactcagg tcaaaacact
    43981 gatttcacaa cctctttttt taatgaaaaa tatggctctc atgcgccgct tctttgaatt
    44041 tcgtcatttt ttcttcgatg aacggataga ttgccgttcc tacgattcca aagacaacaa
    44101 acaaaccgat catgatccag attgctttcg tagcatttgg ccaataaatt cctcctgcag
    44161 attcccgtaa caagtttacc gcatgagtaa atggcaagaa cggattgatc atttggaaga
    44221 atttccctga gacttggatc ggatagttcc cgcctccgcc agagatcgac agtactaaga
    44281 tgataatagc gatcccttta ccgacattat cgaataatgc agccaagaca tagacgatca
    44341 tcatgaacgc taaggcgatc aagacagcaa atgacacgct gtagaccgga ttttggacat
    44401 ctactcctag taagaaaata ttccctaatg taacgatcaa gctttgcgca atcgccattg
    44461 tcaaaaatgt cagcatacga gcaacgaatt gttctcgttt cgtaaaattg tttcggtctt
    44521 tctttcctaa atagtattct gtcgtagcaa cacttgaaag aagaacagcg ccgacccaca
    44581 gacatagtgc agtataaaac ggggtgctag ctgaaccgtt atttgcgatt ggataaagcg
    44641 cattggtttg tagctcaaca ggttgtgtaa agaagtcact ttcttttgtt gcgtctagtt
    44701 tcaataattt gatgacttcg cctagatcga cttcttgttc gccttttcgg atcgcgtctg
    44761 ctgctttttg gataccagct ttgatatttg gccagtcatt gttgatcaag tccgcagcag
    44821 cgttcacagc tgtttcgact tctggcattt tttcatttac tgttgtcaat gtaccggtga
    44881 tttcattttt tagtgctggc caatcatttt ggataaaatc agcggccaat cctaattttt
    44941 gctgaatgat cggtaattca ttgttgtata ggtctgcccc ttcattgatg ccattgacga
    45001 tggtatccat atttccattc agcattgtat tggcatcatg gatttcttgt ccaattgcag
    45061 gcatttcttt ttgatatttt tctaaaattg ccactgcatt cgttacagtt gctttcgttg
    45121 agctgagaag cgaatcaaag tcgatctggt ccgctttgct cagtgcgcct tgtgcagtcc
    45181 caatcgtatc gatgacttgg tctagtagag aagtgacagt ggaactgatt tgacctgtat
    45241 tgataccagc aatgatgcca ttgatattat taacaacggc atcaatttga tctagcaatt
    45301 catcaatagc cttcgtgtct tcattttgaa cagctgtgtt caaagcagat aggcggtttt
    45361 gcagattgct tagcaagggt tttaattgat ttagctgatc aataattggc tgtaatttcg
    45421 tatttccagc acttgtttgc aatttagtca ggaactcgac cagtttatcg attgcatttt
    45481 gttgttgaga tattccgtca ataaagccgt tcaatacatt gctgatgttt ttcttttcat
    45541 catcagtcag cttaccatct tcaatagctg ttcttacagt atctattaac gaacctgtcg
    45601 ttgttgccac ttgtccaatt gacttcaatg taacatctac actgtttgtg atgcttggta
    45661 tcgcatcttt cagctgagag gcacctgatt ttgtttggtt tgctagatca cttgcctgat
    45721 tttctaaatt tttcacatct ggcaagatat cttgtacttg ttggatgatc gtcaagcctt
    45781 gttttgcttc atcgattcct tggttcattg tctcttggat cgaagcaaaa tcgccgtcga
    45841 tctctgctag ttgcttaccg gcattttgga tctctgggat tttttcttgc aaatcaagga
    45901 tgatttttgc ttgatctttc aactcaggca ttttgtcatt caatgcaacg actttttcac
    45961 ccatctcatc tactttaggg atgtaatcca cgaattcatt tgctttattc agtttttcct
    46021 tgatttcagg tagttgactt tgtaattcga ggacttgttt tgtgtagccg tcgatcgtgt
    46081 ccaaattttc atctgttgat aaaatcatat ctttaacttt attgatactt actaggttcg
    46141 tatcgatgtc gtaacctatc tcattgaata ctttcaataa agtcgaactg gctgttttga
    46201 taaattcatt tgtgatttgc gattggatcg aacttgctcc tttatcggtg attttcggtg
    46261 caatcgcatt gattttttca tttaccgtat attcgatttt tggtttcttg atatcaccac
    46321 tagtaaaact cagcagatct tctgagaaat cctttggcaa gtaaatacct gcgtaatact
    46381 ttccggaacg gactccgtct tctaattgtt cttttgaatc aacgaattgc catcctaatt
    46441 gtttattttt atgaagcgat tcgatgactt gctccccaat ggccacttct ttaccttgaa
    46501 actctgctgg cttgtctgca ctgtaaacag caattggcag ctctccagta tttccatatg
    46561 gatcccataa tgctttgatg ttaaaccacg catatagcga agggatgatc atgatcgcga
    46621 tgatcagaag tgttgcaatc gggtttttga aaatccgttt ccagtcaagc aaatataatc
    46681 gaagcgtatt tttgattgac tgcatagttc tcccccttct tcttttttca gctgaattac
    46741 tttaccatgt atttcatact tttttcaatc ggcttttttc aaaaaagtcg aatttttgga
    46801 catatcacgg caattgtctc attgtaatct cttacatatt gagaaataat taaggattac
    46861 agcctttact ttatcatgaa ataaaaggga aagataattt tatgccaaag tttttatcta
    46921 ttatacgtat acgcaaaaaa gctaaaacat tttttgtctt tgcccctaca aaaatgtttc
    46981 agcttttttc gttttttatt cctgtatttc ttatttttcg aatgacagga tgattttccc
    47041 agcaggagga tgtccttcaa ctagctgatg tgcttggatg acatgcttaa gtgaagcaga
    47101 cagattcatt gaaataaaaa catgaaaggc tcctttttgg taggctttga ttatgccagc
    47161 aaaggcttct gcatctagat attcctttct tggaacaaac gattcataaa agccttcctt
    47221 tttttgccgc aaatctagat ctgggaggtc atttaatgca acatacctgc cgcctggttt
    47281 catgatctgg atgccggttt ctcctttgat acttcctttc gtcgcatcga ttacaaggtc
    47341 tgcctgatca gcaaattgca gacctggatt tgttttatca taagcagcaa atgctgaagc
    47401 tcctaatttt tttacctttt cctcattttt actggatgca ctggttaaga ttcggattcc
    47461 cttttcatgg agaagctgga tcagacttga cccgaccgca cctgatgctc ctagtatcat
    47521 tacagtatct gtcggctgga tttccgtcaa atggttgatt agattataag ccgtaattcc
    47581 tggcgtgacc attccggctg cttcttccca actcatttta tctggaattt ttgctacttt
    47641 tgctgatggc aacacgactt cttctccgta agtcccgccg actgcatgga cagctactcg
    47701 atcacctaca tgaacagttg tgacatctga accaatctct gtcacaatac cagcgccatc
    47761 attacctggt acataaggga atttcaatgt tcgtatctct ttcatttctc ctaaacgcaa
    47821 ggcaacatca taaggattca cactgaatgc ttttatttct acacgaacat gtccagaacc
    47881 gacttctctg ggatgagcat cgatttcttc aaaaacatct tgtgcttctc cgtaaccgtt
    47941 aattgcaaat cttcgcatgg tcaccatctc cctttttctt taagtttacg tttatgcctt
    48001 tattttgaca aaaaaaatgt cttttcgttt tggcgcatga ttcaaaacaa aatagttgtc
    48061 ttttttcgcg taacgtgtta aattatttat ctaaaagcgt aaagattacg atttacgcta
    48121 ataaaaaata attggaggaa tttgttgatg gctaaaaaat caaagattgc caaagcaaaa
    48181 aaacagcaag aaacgattga acgctatgct gctttacgcc aggaattaaa agcgcaaaaa
    48241 gattacgaag gcttgcgtaa acttccttta gatgctcgtc cagaacgatt gaaaaatcgt
    48301 gatttaatcg atggacgccc tcgcggatac atgcgtaaat tcgggatgtc ccgtataaaa
    48361 ttccgtgaat tagcacataa aggtttgatt ccaggagtaa aaaaagccag ctggtaatcc
    48421 aagtctggct gtaatgtaaa ggaggagggc gcatggacca ctccttggat catcaaaaaa
    48481 gatacgttcg cttcttctag cagaaatgct ggtactagaa gcagacgtat ctttttttac
    48541 gcacagctgg tcctttttta tatgtaaagg ctgcatggtt acttcgctat atttatttag
    48601 taacaggagt cagtgcttcg atacccacac taattttcaa tgctcggtca acttctgcca
    48661 taatgggtcg atccaaatgg cagacctttt ctttcaatct tgatttatcg atcgttctga
    48721 tctgctccaa caggataacg gagtctcttt ctattcctgt tccagtagct ttgatcccaa
    48781 tgtgagtcgg aagcttaggt ttagccattt ttgccgttat ggccgcaaca atgatcgtcg
    48841 ggctaaaatg gttacctaga ttattttgaa taaccaaaac tgggcgtgtt ccgccttgct
    48901 ccgatcccac gactggagaa agatcagcaa aataaatatc tcctcttttt accataggat
    48961 cctccttatt cttgatattc tcttggtatc cgctgtccta atccgcaagc cacttcatag
    49021 tggatcgtac ctaattgatc tgctatatcc tgcatagtga tttctttgtc tgcatttttg
    49081 ccgataagcg tcacttttgt tccgacagga aattcttgag gcaatcggat catcagttga
    49141 tccatacata cacgcccaat cgtttcacag tagttccctt ctaccaatag agaaaaaccc
    49201 tgcatctttc gtggccagcc atctgcataa ccgatcggta tcgttccgat ccactctgct
    49261 tcaggagtga tatatgtttc tccatagccg attccttcac ctgctgggag ttttttgact
    49321 tgaatcaatt cagaaaccag ctcaagtgct ggctgcaatg gataaacttc cggtagtgcg
    49381 tgcccagagg gattaagtcc gtacattgct actccataac gaatcatatt gccgattgtt
    49441 tcatcatgcc ataaagccgt tgcactattg ctgacatgta catatcgagg aagttcggga
    49501 agttttttca gcacttctat aaagcgttct ttttgcagat tccaatatgt atcatctgcc
    49561 tgatctgctg tagaaaaatg ggtaaataaa ccttcccatt ctaatacacg actttctttg
    49621 atgaatgcag ctgcctcttt tacttcttca ggtgtacaaa atccgatacg acccatccca
    49681 gtgtctgctt ttagatgaat cgaaagaggc gtctctagct tacttttctc taaaacagca
    49741 gctgctgctt gcaaccattc ccgtgtacct gcagttactg ataaatcatg agcaacagct
    49801 acagtcagtg cgtcgtaagg aaccatattc aagataagga tgggctgagt aaatccagct
    49861 tctctcaact cgactccttc atctaaattt gaaacacaaa agcccgaagc tccacctgca
    49921 actgctgcct cagctgtttc aatagctcca tgaccgtatc cgttcgcttt tactactgca
    49981 aacaattctt ttccttgcgg aagtcttgca acttcatttg ctacattttc agttatagct
    50041 tttttatgta tcactgcttt cgttgggcgg tgccaagcga caaccatcgt ttttccttct
    50101 ttctagtctt ccaaaataat ttgtgcaaaa gctgtttcat ttgtatgagt aatactgacc
    50161 catacattgc cttcatgagg tgaacgcgtc acaacaggct gaccattttc attttttaaa
    50221 atctcgacat cttgaaaggt aacttttccg atcccagttc cccaagcttt agagaaagcc
    50281 tctttgcaag cgtaacgccc gcctaaaaat tctacttggc gatgaaaagg caaggactgg
    50341 aatagtttca tttcatcaga ggtcaagatt cgtgcaatga attttggttt ttcttcgatc
    50401 agccgagtga tcctaggaag ttcgactgca tcgatcccaa ttccttttat catatgcttt
    50461 tctcttttca ataaatttag aacactctca ttctaccaaa ttataaccat tttgccacaa
    50521 ataagtccac aaataacaaa aatgaaagtt tctcttaaaa aagagaaact ttcattttat
    50581 gtttaatcgt tatttgaacg gattacaaat ccgcgttttt tatcattgtg acgtttaccg
    50641 ccatcttttt ggctgttatt atttttgcta taaccgcctt ttgatttgtt tccacgataa
    50701 ttaccgccgt cacgacgatt acggttgttt ccgccaccac gtttgccatt tttattgtat
    50761 ccttttttct gcattggcaa tgggcgttct ggtgtgatct tcactggcac tgcatcagct
    50821 ggatcttttg ctgtcgtttt tagcaaaagt gcaactaaat cttgtgcaga atattcttct
    50881 aataatttat ctgctgtttg caggtattta tctagaccat tttctgcaag ttttgtttcg
    50941 atttgttcaa ctgcagcacc taattgacct ttgaatgctt ctttttcggt tggtggacgt
    51001 aaagtcgtca tccgtttttt cgtcaagttt tcaatcacat gcaagtagtc catttcattt
    51061 ggtgtaacga aagtcacaga cataccgcct tttccggcac ggcctgtacg tccgatacgg
    51121 tgaacgtagc tttctggatc ttgagggata tcatagttgt acacatgagt gacacctgaa
    51181 atatctaatc cacgtgcagc aacgtctgtc gctacaagga tatctaaatg accgctctta
    51241 aatgaacgca atacgctcat tcgtttttgt tgagacaaat cgccatggat tccttctgca
    51301 cggtaaccgc gtgcttctaa tccgcgtgcc aattcgtcta cacggcgttt tgtacgtccg
    51361 aagacgattg ttaattcagg tgtttgaaca tcaaacaagc gagtcatgat atcgaatttt
    51421 tcgtattctt ttgcgcggac atagtattga tcgatcaagt cagctgtcat ttctttcgct
    51481 ttgatcttca catgatgtgg atttttcatg aatttaacgc caatattctt gatagctggc
    51541 ggcatcgttg ctgagaaaag taatgtttgt ctttgatcag gaacttgtga aatgattttt
    51601 tcgatatctt ccaagaagcc catattcaac atttcatctg cttcatccaa aactaatgtt
    51661 tgaacggttc caagtttcaa tgtgtggcgg ttaatgtggt caagcatacg tcctggtgta
    51721 cccacaacga tatgtggacg atctttcaag ccacggatct gacggccaat atctgctccg
    51781 ccgtaaacgg cttgtacacg tacttttttg tcgcgcccta aacgatataa ttcttcttgt
    51841 gtttggatag ctaattcacg tgttggtgcg ataactaaac cttgtaattc gtgacgatct
    51901 ggatcgattt tttctaacat tggtaatcca aatgcagctg ttttccctgt acctgtctgt
    51961 gcttgtccaa taacgtcttt tccagctaat gctagtggaa tcgtttctgc ttggataggc
    52021 gttgcttctt caaaaccagc gcgttcgact gatttcaaca attctggtga taattctagt
    52081 tctttaaatt tcaaatgaat cctcctatat ggttgttaac tgaggatgta gcttttttgc
    52141 taccccgtcg atttgttcgg tcaaagtgta ttgtttcttt ttgacagtgg ccacgcacgt
    52201 ttttatccat tcttagcccc tgtggcacca ccgaaaaatc actttgcgaa gtttaggcat
    52261 tagaaaagcc aaacgggtcg cgttcagctt tatcattatt atgacatctg gcttcaacaa
    52321 gttgtttcta tggcaaaacc agtccaattt ctcttcatct ttcggcaata ggttattcta
    52381 acatagtttt tcgttttctg caagtttaca gatttttatt tcatttgctg aagttgttct
    52441 accacttcaa ataagcccat gctgttgctg ccttttagca taatactatc tgttgcttca
    52501 agattttgtt tgatagcatc aatcaattta ttcttttctt tttcaaaata gaacacaggt
    52561 agttgggggt atttttggtt caattcttct tttagtgcat gcatttgttc cccataaagg
    52621 aaaatcatgg cgaatttctc aggatcgata tgttcactca tttgcgcatg catatttaaa
    52681 gactctttcc ctaattccag catatctgct aacacagcga ttctgcgtcc ttttgttggc
    52741 agatttttaa aagtatctaa taccagtccc atggctgttg gatttgcatt gtagacatcg
    52801 ctcaaaatag ctgcaccatt tcctgccgtt agccattcgg ttctgttttg agtgatagaa
    52861 acatgggcga gcccttggcg tatctcaggt atggacagtc caaagaagcg cccgatcgta
    52921 tacgcaatca aagcattcgt tacattataa cttccaggca gcgggatcgt aaatgtttct
    52981 ccttctacta caaaatcggt ttgtgctttt ccttttgctg tcacttccgc atgaacatcg
    53041 ccatttccaa taccgaatgt tgtgactgtt tgtactaatg ttgccgttaa aggtgtcagc
    53101 aacggttcat ctgcgggcac aacgagtaat ccgtctgata caagtccgtc agtgatttcc
    53161 attttggctt tagcgattcc ttctcgcgac ccaagatttt ctatgtgtgc ttcaccgatc
    53221 attgtgattg ctgcagcttt tggttgagcc ataagtgaaa gttccgtgat ttcattggcg
    53281 tgatccatgc ccatttccaa aacgatcttt tctgtatctt ggggcatgtg caaaatcgtg
    53341 taaggaagac caatattatt gttgtaattt ccttgcgtct tatatgtttt gtatttttca
    53401 gacaagacag acgcggttag atctttcgtg gtggttttac cattgcttcc tgtaattgca
    53461 acgacttctg cgcctacttt ttccaagtaa taggttgcca attgctgcat ggctttcaaa
    53521 ggatctgcta caggaataac tggaaaattt tttggagctt gttcaacagg caagctccaa
    53581 aatgaagcag atgctccgtt tgtgatcgct tgctggataa attcatgtcc atctcgcgta
    53641 ccagcaagag gaacgaataa acttccttcg gttatcttcc ggctgtcaaa ttcgacattg
    53701 gtaatctttg tcagtgcaga gcattcgctg cccacggctt gtgcaatttc ttgtattgtt
    53761 agattcattg agatctctcc taaaaaaggt tgtgaaaaaa gcgtttggcc taaacggcgg
    53821 gttcgcatga ctctcacttc caagtaaatt tctctattcg tatttagaag ctagaatgaa
    53881 cgcggttacg caatcactcc agcctcttct ttatttctct ttattcattt ttttcaaaaa
    53941 aagattgacg ctgatggtat cgattcattc ctaactggat cagttcttca atcaaatcac
    54001 cgtatttcaa gcccatattt tcccataaga gtgggtacat actgaactcc gtaaatcctg
    54061 gcatagagtt taattcattc aggaataatt catttttatt tgtcaaaaag aaatcgcacc
    54121 ggctcaatcc gcttccacct aacatcgtgt aagctaactt cgcgtactct tgcgcttttt
    54181 gataaacttc ttctggcact tcggctggaa tctgcatttc gattttatta ttgatatatt
    54241 ttgcttcata atcatagaat gctacgtctt ttacgacttc accaggcaaa gtcgtccgaa
    54301 cgtcttcatt tcctaataca gcaacttcga tttcgcgcgc ttcaattcct tgttcaacga
    54361 ttgctcgaga atcatactga taggctgttg ctaaagcatt ttgcagctct tctcggtttt
    54421 ctgcttttgt aatgccgaca ctagaaccca tattcgccgg tttgacaaac atcggataaa
    54481 gcaaagaacc ttcacattga tcaaatactt ttttaggatt ttctttccat tgattcttaa
    54541 gtactggtac ataaggaact tgcggcacac cagcagcttg taaaatatac ttggtcatga
    54601 ttttatccat ggcacatgca ctggtcaata cgcctgcgcc gacataaggc atattcaatg
    54661 tctctaagaa gccttggatc gttccatctt ccccgtttgg cccatgtaaa actggaaaaa
    54721 cgatggctcc ttcttctttg atttcgcccg gattgatcac ttttcctgta aagccttcct
    54781 ctgtctgtcc acttgggtcc catgaaagat gcaagacatc tttgctggca ggtttttctg
    54841 ttagtaatgg acctttgacc cattgtcctt cttttgtaat aaatacgagt tgaacttggt
    54901 aataattata ataaatggca tttaaaactg aaaatgcgga aagaatggac acttcatgct
    54961 ctgcgctgcg tccgccatat agtaaagtaa tcttcaaaag attgtcctcc tgtattctaa
    55021 atcgttcagt gatttactct catattttag cacaaaacaa aaataaacga ataacttatt
    55081 tataaaaaaa cgagccagct tctaaaagaa cgagcgtcat ttattttttg tcagctagat
    55141 tatttgtccc gttttttata aaaagttatg ataaagaaaa ggagggatga tgatggcgca
    55201 aacaaatcca cgcagccgct tggcagaaat cattcgagtg ttcatcaatt acaatgtagt
    55261 gccaaacttt gtacagcaaa aaaatcccga acaggtaaaa aaagcctttg aagaactagg
    55321 ccccaccttc atcaagatag gacagatgct ttctgttcga gaagatttgc tttcttctgc
    55381 cttcactcaa acattcaaaa cattacaaga tagtgtacct agcgacacat tttcaacggt
    55441 caaaaagacg atcgaaacag aactttctct ttcattatca gatatattcg atgatttttc
    55501 aaaatcgcct tttgcctctg cttctatggg gcaggctcat cgtgccaagc tgaagaacgg
    55561 tgacgcggta gtcgttaaga tccaacaccc taacatcgcc gaagagatcc gacttgatct
    55621 tcaactattt gaacgagcga tccctttgat caaatacatt cctgaaacta gcgtagttga
    55681 tctaaaaggg gtactccaag aagtaaaacg ttccttgatc aatgaaatgg acttcttaaa
    55741 agaaagtcaa aatggcgaac aattctatca aaagaacaat ggctggaaag agatccgcag
    55801 ccccaagatt tatgacgctt tttgttcgaa aaaagtgatc gtgatggaag aaatgtctgg
    55861 taaaaacttg aatcatttaa tgaacgccga aaacaaaact gaaactttta tcactggaat
    55921 tcaaaacaag cagctgaaac aagaagttgc aaagctttta gttgagaact ttatgaaaca
    55981 ggtgtttgat gatggttttt tccatgctga tcctcatcca gggaatctgc tattccatgt
    56041 actaacaaaa gaagaacaga cacaagctag ccgaaaaact gaaacagtcc atgaaaaaga
    56101 gttcggttcc tttgcttttc gagcctccac ttctgcagaa gaccctgttg cgccatatac
    56161 catcaactac atcgattttg gtatgatggg gcatctttct gcaggactta ggcaaaaact
    56221 cacacaagcc gttttagcgc tgtatacgaa agatgcttat cgaatcgaaa aagcggtcct
    56281 tcgactttgt cagcaagaag gctcgtttga tgaaagtcgg ttccaccaag aactgacaag
    56341 ttttcttgaa cagtattatg attctcctat tgatgaaatc aacttgcaag aagtattcac
    56401 acatgtcgtg acgatttgtc atcaaaataa tctgcagttc gatcgtgata tcacgctttt
    56461 gctgaaagca tttgggacat tagaaggggt gatccgcgtc ctcgatcctg aagtttccct
    56521 catggaagtc gcttcgcctt ttgctcaaca ttattttctc actcatctgg atgtagaaga
    56581 tacattgaaa caaagcggat tagacttatt ggaagggatg aaggctgcgc ctaagatccc
    56641 tcaacagctc caccatctat tagaaatgtg gacttcggga caaggaaaag tcaatttgga
    56701 attgaaaaag caagacaaat tattgagcag gatcgaaagt atgatcaatc ggcttgtttt
    56761 cggtatgatt ttagctgcat tgatcgtagg gtcctccctg cttgtccaag cagcacctgt
    56821 agaaaacgca gaggtcgttt ccttattagg gatatttacg tatgctattg ccgcattcgt
    56881 cattattttt ttggcaatcg atgcattgat ccaaatgtat aagaagagga aaaagtagaa
    56941 aaaggttgtg acagaaagaa tcagcttcaa gaaataagaa ggggttttac ataaattgtt
    57001 tctcaaattt ttgtgaaaat tcggcttatt tttcgaagag gctacttctg aaacaccgag
    57061 aataaaaaat gtgaaaaagc tgttctgcac ccagtaataa gaacaatcaa aaagggaaca
    57121 tggaacacat ttatcctgtt tccctgttcc ctttttgatg tattcgagca taaataaaat
    57181 accttcgtta cctactatct tctgtacttg gtaaacctta gtcaattgcc ttttttaacg
    57241 cttgacgaaa agacatatga tctttctccg taaagggttt tggacccttt gtccttatgc
    57301 cagcttttct taactttcca ctatgataac gttgttctag aagcgtgccg atattcgctt
    57361 cgtcaaattc ttttccagag tgatggaaca ctcgcacacc cttcggcaat acaagtgaag
    57421 ctaaaccata atcttgcgtg atcaaaaagt cattcttctt aaccataccg acgattttat
    57481 aatccgcact atcagctccg cggtctacgt agacgatttg gacatgatta gcgtattctt
    57541 tggtcgtata atggtccaca ctggtcacga taaccacgtc aagaccatag gattctccta
    57601 attttatgac atcttctttt actggagaac catctccatc aatgaatagc ctcattcttt
    57661 ttcgccttct tccataaatg ataaacagtt actaatccgc tcagtaaaag accaacattg
    57721 atccattttg ccaagataag cagttgtaca ctttctatcg gcactgattc atggttcagc
    57781 gtgatcaata atagatggat tggctctccc atttcttttg ctaaaacaaa caaaagcgtc
    57841 atcaataaaa actggtggat cacactccct ttattttgag tagtatactt gacaacgatc
    57901 cggcgatcca ttggaatagc tgtatcagtt tgataatatt ccttatttag ccagctgcct
    57961 gttactacgt gccagaaact aagcagaaag acacctatcg caaaaccatt tggaatcaag
    58021 aggaccaata aagaagaact cagtccaagc aattgcatag ccaatggaga ccatgttaaa
    58081 cgtttcaaaa tccatggtcc gactattttt gctgcaatca agcctgctgc ataaggcaaa
    58141 tacataaaaa tcgaaagttt ttctaatccg tacagtatgc caacataaaa actgctaaat
    58201 aaaaaaagga aattccctag cattccattt agaaaagtaa acagatttag ccagcttggc
    58261 aacttccatt ccttcttctg gtgtcctaaa acaaacacga acaagatgaa cagcagcaag
    58321 aaaccataaa tagcaagatc aaaaagcctt tcatcgaata acaatcgggc atttctcaaa
    58381 agaaacaata agccaaagaa tatcaaaaat aaaacaagct ctcgcgtagc taccagattt
    58441 cttttgatgt ctttgaaatc cagttcgtaa cgcggataat gactaaccgt atggtaggcc
    58501 atgacgtaaa aaagtgtata aatagtaaag cttgctgcaa tctgagtcgg tgcaggcaac
    58561 tcaatcgctt gatacaggag aaatagccag atagctgcaa ataagtactt tttcccatcc
    58621 atattgataa aaccttgcgt cttttcgtga aaattgaccg tggtattagc aggtacaagc
    58681 catgctgcac tcagccctag caaaacactt gaaaaataaa ataaaggaaa ataaaatacg
    58741 cctaatattc caagcaatga accagcacca ccaatcagca gagcgatcat cagcaacgtg
    58801 taactatcta gaccaaatcg tagactgttg actaaaaata gtccagtcat ccgaaaggca
    58861 taaaacagcg taaatggcaa aatcgtcagc caattatgcg agtctgttag tcccaaaaat
    58921 aaaagataag gaataaaaat aaccgtgtta cttaagataa agggcgttgt ttgtgtaaaa
    58981 ttacgcccaa atcgtttgag tttttcgaac atgacaatgg ctccttaaac agtcttataa
    59041 ctaatctgct cttcgatttt agcattaaag gggaaataaa ccaattttac agaatatatt
    59101 cttgtaaaaa aaaatacaga aagaagcata agcttctttc tgcattttta cggtacttga
    59161 ttactcaagc agacaagccc ttctccacaa aagtcttgcc cgacacccct gcatccggca
    59221 ggtgataaag atagaaatca attatcctgt gcctccaaaa tcaattagga gctctgtaaa
    59281 ccagagtcct tctcgacccg tcgcataaat ttactttaac acagttccta tgtttatgcg
    59341 agagcttttt tcattttata atcgaccatt tgaaatcctt gaccgatatc tgaaataagt
    59401 tcttctgtaa tctcaaatcc ttcatgctta taaacctcga ttgcttgatg attcccctga
    59461 ttcactcgca aaaagatctc tctttccttt gtttgcgcag caagttcttc ataccagcga
    59521 aaaactgaag aagtcaatcc ttgaccacgt actttattta gcaaatataa tttacttaaa
    59581 taaatttttc catttatctc ttcataagca gtgtaaccga cagtcttttc ttcgcttttt
    59641 aataaaaagt aggacacacc ttctgtcagt tctttttgaa tctgtttttt tgactgataa
    59701 gttttcagca tgtaatccac ttgtttttga ccaatgatcg gcacaaaaac ttccggccat
    59761 acttcttgta tgactgccat cagctcatcc agttcatttt ctgttacttt tattttttcc
    59821 atacacccac gtcctttttt acattataaa tgaccttgcg cttgatggaa taaaaagagc
    59881 atgatcatgc tatacacacc caccgaccac ggtttcaaga aaatcacaat ctgcataaat
    59941 tccctaaaag ataatttcgt caaaccagct actagacaga tcaagtcgtc tggcgcaaag
    60001 ggaagcagca aggtaacaac tagtactttt ttaatatctt tcgtttttct tgtcagcaat
    60061 tcatcaaatt tctgatactt ttttggcgat aaaataagct ggacaaaggc tacgccatag
    60121 taacggacaa gcaaaaatcc gattgcttcc ccaatcacta ggccaataca actatataaa
    60181 agcccaaatc cattgccaaa catcagcatg cccgctactg aggagatccc tcccggcaaa
    60241 ataggaatga tcacttggat cacttgcaac agaacaaata tcatcactgc atacttgcca
    60301 aattgctgga taaacgcttg caaagacgtg ctcgatcgaa aaatcccatg atgataagca
    60361 taaagaatca acacaaagaa aaaaaacaga ccaataatgg gcatgatctg aagacatttt
    60421 tgtaaaactg atcctttttt agacatctat attccccaca tcttttttca tttattaaaa
    60481 ctgcctcctt
//
LOCUS       DACCCS010000009        58971 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.009, whole genome shotgun sequence.
ACCESSION   DACCCS010000009 DACCCS010000000
VERSION     DACCCS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.009"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            214..1500
                     /gene="efmA"
                     /locus_tag="IVK82_000603"
     CDS             214..1500
                     /gene="efmA"
                     /locus_tag="IVK82_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP6391686.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1638..2237)
                     /locus_tag="IVK82_000604"
     CDS             complement(1638..2237)
                     /locus_tag="IVK82_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP6391687.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2641..3762
                     /gene="mnmA"
                     /locus_tag="IVK82_000605"
     CDS             2641..3762
                     /gene="mnmA"
                     /locus_tag="IVK82_000605"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP6391688.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            4008..5429
                     /gene="arcD"
                     /locus_tag="IVK82_000606"
     CDS             4008..5429
                     /gene="arcD"
                     /locus_tag="IVK82_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP6391689.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5443..6561
                     /locus_tag="IVK82_000607"
     CDS             5443..6561
                     /locus_tag="IVK82_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP6391690.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6621..7913)
                     /locus_tag="IVK82_000608"
     CDS             complement(6621..7913)
                     /locus_tag="IVK82_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6391691.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            8063..8623
                     /locus_tag="IVK82_000609"
     CDS             8063..8623
                     /locus_tag="IVK82_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6391692.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8752..9129
                     /locus_tag="IVK82_000610"
     CDS             8752..9129
                     /locus_tag="IVK82_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP6391693.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            9062..11134
                     /locus_tag="IVK82_000611"
     CDS             9062..11134
                     /locus_tag="IVK82_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6391694.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11345..12292)
                     /locus_tag="IVK82_000612"
     CDS             complement(11345..12292)
                     /locus_tag="IVK82_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6391695.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12538..13002
                     /locus_tag="IVK82_000613"
     CDS             12538..13002
                     /locus_tag="IVK82_000613"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP6391696.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(13064..14107)
                     /locus_tag="IVK82_000614"
     CDS             complement(13064..14107)
                     /locus_tag="IVK82_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP6391697.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            14458..15321
                     /locus_tag="IVK82_000615"
     CDS             14458..15321
                     /locus_tag="IVK82_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6391698.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(15450..17072)
                     /locus_tag="IVK82_000616"
     CDS             complement(15450..17072)
                     /locus_tag="IVK82_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6391699.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            17321..17842
                     /locus_tag="IVK82_000617"
     CDS             17321..17842
                     /locus_tag="IVK82_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391700.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(18028..19758)
                     /gene="spxB"
                     /locus_tag="IVK82_000618"
     CDS             complement(18028..19758)
                     /gene="spxB"
                     /locus_tag="IVK82_000618"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP6391701.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            19923..20741
                     /locus_tag="IVK82_000619"
     CDS             19923..20741
                     /locus_tag="IVK82_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP6391702.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(20844..21041)
                     /locus_tag="IVK82_000620"
     CDS             complement(20844..21041)
                     /locus_tag="IVK82_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391703.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            21210..21788
                     /locus_tag="IVK82_000621"
     CDS             21210..21788
                     /locus_tag="IVK82_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP6391704.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(22833..23336)
                     /locus_tag="IVK82_000622"
     CDS             complement(22833..23336)
                     /locus_tag="IVK82_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6391705.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            23695..24720
                     /locus_tag="IVK82_000623"
     CDS             23695..24720
                     /locus_tag="IVK82_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6391706.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            25043..25921
                     /locus_tag="IVK82_000624"
     CDS             25043..25921
                     /locus_tag="IVK82_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391707.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            26397..26513
                     /locus_tag="IVK82_000625"
     CDS             26397..26513
                     /locus_tag="IVK82_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP6391708.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            27441..27779
                     /locus_tag="IVK82_000626"
     CDS             27441..27779
                     /locus_tag="IVK82_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391709.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            27873..28103
                     /locus_tag="IVK82_000627"
     CDS             27873..28103
                     /locus_tag="IVK82_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391710.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            28503..28874
                     /locus_tag="IVK82_000628"
     CDS             28503..28874
                     /locus_tag="IVK82_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP6391711.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(29148..29840)
                     /locus_tag="IVK82_000629"
     CDS             complement(29148..29840)
                     /locus_tag="IVK82_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6391712.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(29843..30628)
                     /locus_tag="IVK82_000630"
     CDS             complement(29843..30628)
                     /locus_tag="IVK82_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6391713.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            30865..32265
                     /gene="lacG"
                     /locus_tag="IVK82_000631"
     CDS             30865..32265
                     /gene="lacG"
                     /locus_tag="IVK82_000631"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP6391714.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            32290..32607
                     /locus_tag="IVK82_000632"
     CDS             32290..32607
                     /locus_tag="IVK82_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6391715.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            32620..34293
                     /locus_tag="IVK82_000633"
     CDS             32620..34293
                     /locus_tag="IVK82_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6391716.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            34307..35938
                     /locus_tag="IVK82_000634"
     CDS             34307..35938
                     /locus_tag="IVK82_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP6391717.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(36429..37994)
                     /gene="guaA"
                     /locus_tag="IVK82_000635"
     CDS             complement(36429..37994)
                     /gene="guaA"
                     /locus_tag="IVK82_000635"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP6391718.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(38053..38976)
                     /locus_tag="IVK82_000636"
     CDS             complement(38053..38976)
                     /locus_tag="IVK82_000636"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP6391719.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            39172..39777
                     /locus_tag="IVK82_000637"
     CDS             39172..39777
                     /locus_tag="IVK82_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6391720.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            40085..41101
                     /locus_tag="IVK82_000638"
     CDS             40085..41101
                     /locus_tag="IVK82_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6391721.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            41242..42543
                     /locus_tag="IVK82_000639"
     CDS             41242..42543
                     /locus_tag="IVK82_000639"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP6391722.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            42589..43251
                     /gene="deoC"
                     /locus_tag="IVK82_000640"
     CDS             42589..43251
                     /gene="deoC"
                     /locus_tag="IVK82_000640"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP6391723.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            43272..43670
                     /locus_tag="IVK82_000641"
     CDS             43272..43670
                     /locus_tag="IVK82_000641"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP6391724.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            43736..44818
                     /locus_tag="IVK82_000642"
     CDS             43736..44818
                     /locus_tag="IVK82_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6391725.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            45030..46592
                     /locus_tag="IVK82_000643"
     CDS             45030..46592
                     /locus_tag="IVK82_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6391726.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            46585..47718
                     /locus_tag="IVK82_000644"
     CDS             46585..47718
                     /locus_tag="IVK82_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6391727.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            47718..48674
                     /locus_tag="IVK82_000645"
     CDS             47718..48674
                     /locus_tag="IVK82_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6391728.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            48773..49939
                     /gene="deoB"
                     /locus_tag="IVK82_000646"
     CDS             48773..49939
                     /gene="deoB"
                     /locus_tag="IVK82_000646"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP6391729.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            49963..50781
                     /locus_tag="IVK82_000647"
     CDS             49963..50781
                     /locus_tag="IVK82_000647"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6391730.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            50798..51505
                     /gene="deoD"
                     /locus_tag="IVK82_000648"
     CDS             50798..51505
                     /gene="deoD"
                     /locus_tag="IVK82_000648"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6391731.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(51803..52489)
                     /gene="gpmA"
                     /locus_tag="IVK82_000649"
     CDS             complement(51803..52489)
                     /gene="gpmA"
                     /locus_tag="IVK82_000649"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP6391732.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(52661..53242)
                     /locus_tag="IVK82_000650"
     CDS             complement(52661..53242)
                     /locus_tag="IVK82_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6391733.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(53375..54058)
                     /gene="rpiA"
                     /locus_tag="IVK82_000651"
     CDS             complement(53375..54058)
                     /gene="rpiA"
                     /locus_tag="IVK82_000651"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP6391734.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            54377..54790
                     /gene="rpsL"
                     /locus_tag="IVK82_000652"
     CDS             54377..54790
                     /gene="rpsL"
                     /locus_tag="IVK82_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP6391735.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            54874..55344
                     /gene="rpsG"
                     /locus_tag="IVK82_000653"
     CDS             54874..55344
                     /gene="rpsG"
                     /locus_tag="IVK82_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP6391736.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            55438..57525
                     /gene="fusA"
                     /locus_tag="IVK82_000654"
     CDS             55438..57525
                     /gene="fusA"
                     /locus_tag="IVK82_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP6391737.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            57697..58884
                     /gene="tuf"
                     /locus_tag="IVK82_000655"
     CDS             57697..58884
                     /gene="tuf"
                     /locus_tag="IVK82_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP6391738.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT        18484 a        10568 c        12399 g        17520 t
ORIGIN      
        1 ttgtaaggac gacgcaaatt agttgtcttt tttattttta aatttaacac atgttgttat
       61 ttttaatatt ttgctgattt attgtttcta agtaaagaaa tttaaattga aacgattgtt
      121 atttagggaa aaaggtattt tttgaaaaaa gaatcccgta taatgattgt tgtgcaaacg
      181 ctatacaaaa tgaaaaagaa aagagggatg tttatggaaa atgaacagtc ggtcgtttta
      241 acgaattgga agagaaatta tttgttcttt ttatcgggac agtttttgtc aggaatcact
      301 agtatggtcg tccaatatgc gatcatctgg tatttgacaa gagaaaccgg ttcggcgaca
      361 attttgagtt ttgctacttt actaggaatg attcccatgg ttttactaag cccatttgta
      421 ggaccgctag tggatcgctg ggataaaaaa gctcttttga ttgtcacgga tattattgta
      481 gcgattttcg cgcttatttt agcagtagtc ggaacgattt cagaatcctt cccgatttgg
      541 ctagtttttg tgtctttgtt tatgcgttcg gtcgcacaaa cattccaaat gccaacgatc
      601 cagtcgatta tgccgacaat cgttccatct tctcacataa caaggacgaa tggacagtta
      661 ggaatggtcc aatcagcgaa tttcatcatt gcgccagctc taggtgcggc tttgttttca
      721 gtcgttcctg tcaattacct tattttatta gatgtattgg gggcagtttt tggtgtcggc
      781 ttattgattt ttgtgaagat cccaaaagtt tctcctgaaa tattggaagt tccgcttact
      841 atttttaaag atgcaaaatt tgggctgcag caattgatgg ataataaagg gttatggtac
      901 ataacgatca atggtgcatt tgtgatgctg ttgttcatgc cagcaatcag tctgtatcca
      961 ttgatgacgc tagattactt tggtggttcc gttggacagg cgggagcagt ggaagtagtc
     1021 tacgcagtcg gaatgctctt aggcggtgca ctgatcagtt ttataggaac atggaaagac
     1081 cgaatgaaac ctattattat agcctacatc attatgggcc tgacgatcgg tgcaagcgga
     1141 ttggttccaa acgatagtca agggttcttg tactttctta tcttgaatgc aggagcaggt
     1201 tgtgcaacgc catatttcaa tacactgctg atggcaatga tccagcagag ttatgaatcg
     1261 aatgttttag gtcgtgtctt agggaacttc aactctttga tgaacctggc aggtccaatt
     1321 ggattattat ttgcaggacc attagctgat cgtttagggg tagaaaaaat gttcttgttt
     1381 tccggaatcg ggattttatt atgcggaatc gttttatttt taacgtcagc tgcccgaaat
     1441 tatgataaag aattacagaa aaaactggta aaagaacacc atgagcaaaa agacgaatag
     1501 tatggaatgg agttgtgaca gaagtcttgt tacaactcca tttctgatcc taatgttcct
     1561 gtggcaataa gcctactcaa atgaaaaata tgagtaatta tctttatttg tttaatactt
     1621 gatatagggc agttttttca cgacctatcg tacttcctct ttgcttcata gatttctttg
     1681 atttgttgct cagataacgg agtgccaaat ggtagttttt cctttaagag ttctctttcc
     1741 ggtgcgtcta cgcctacatt gacatcgttc tcgataggta cgctgtaatg atggatatgt
     1801 ccatgaagat tgcggatagt gtcgttaggc ccgagcaaca tcggataatg agtcaagtaa
     1861 tattgttgat ggtcgaattt aacgatggca cctacatcat aaaaataaaa tttgggactt
     1921 ccgtcagtaa gcctaggatc atttttttct aaataacgga aaaaagcgcg actgtcatgg
     1981 ttccctttga tcaagtggat tgttccttgc agttgagtga ccagttccaa aatttctgca
     2041 tttgcttttt catattgcgg atgcatagca aggtcgccta gatgataaac atggtcggtt
     2101 tcttttatta ctgcattcca gctagtgatc aactgttggt tcatttcatc cacatttgca
     2161 aagagccgcg gagcaaagtc gttttttcct aataagcgtt catggaaaaa gtgcatatca
     2221 ctgataaagt aattcatgta tcttttgcct cccttttttc ttagagtaaa caaaatcact
     2281 tatctgagca actcataaca tctaaaaaga aaaggttgta aatgagacgt gcagctttaa
     2341 gcgataagaa gcaattttga aaaatagttt ttcacaattt ataaaattat tccttattgc
     2401 cgaaaggctg gcccttttta tgagtatgcc aattttttat atcgaatgct caaaagatga
     2461 gtgatttttt ggttgactat catgtttgtg tactgaatag gtccagtaga acagtatttt
     2521 tacgacttga taattaaggg atgaaatttt cagaaatatg acgtataatg taaaatactg
     2581 aaaagttgcg accttcaact cgtagatgtt tcagaatcta attgaaatga tggtgatatc
     2641 atgacagaca acagcaaaac acgcgttgtc gtagggatga gcggcggtgt cgactcatct
     2701 gtaacagcac ttttgttaaa ggaacaagga tacgacgtga tcggaatatt catgaaaaat
     2761 tgggatgaca cggacgaaaa cggtgtatgc acggccacgg aagattataa ggatgtagcg
     2821 aaagtagctg ctcaaatcgg gattccttat tactctgtga attttgaaaa agaatattgg
     2881 gatcgtgtat ttgaatattt cctagccgaa taccgcgcag gacgtacacc taacccagac
     2941 gtgatgtgca acaaagaaat caaattcaaa gcattcttgg attatgcaat ggatcttggc
     3001 gccgattatg tagctactgg acattatgcg caagtcactc gcgatgaaaa cggcgtggtc
     3061 cacatgcttc gtggagtaga caataataaa gatcaaactt acttccttag ccaattatca
     3121 caagaacagc tttcaaaaac gatgttccct ctaggcggca tggaaaaatc agaagtacgg
     3181 gcaattgctg aacgcgcagg ccttgcgact gctaagaaaa aagattcaac gggaatctgc
     3241 tttattggtg aaaagaactt caaacaattt ttgagcaact atctgccagc aaaaaaagga
     3301 aatatggtca ctcttgatgg tgaagtcaaa ggacaacatg ctggcttgat gtattatacg
     3361 attggtcaac gtcaaggact agggatcggt ggcggaggag attcgcaaga accctggttt
     3421 gttgtaggaa aagatctagc gacaaacaca ttatatgtgg ggcaagggtt ccatcatcct
     3481 gcattgtatg ccacaagctt ggatgcaagt gaaatccact ttacgacgaa tgaaccaatg
     3541 ccaaaagaat tcaagtgtac agcgaaattc cgttatcgtc agcaagacgt tcctgtgaca
     3601 gttcgcttat tggatgataa tcgtgcagaa gtcgtattcg acgaacctgt acgtgcaatc
     3661 actcctggac aagcagttgt attttatgat ggaatggaat gtctgggtgg tggtttgatc
     3721 gaccacgcat atcaagaaac aaaagtttta caatacgttt aagcgatatt aaatgaatca
     3781 aaacagctat ggctaacaaa agaaacacct tttcttttgg atagcatagc tgtttttttg
     3841 caataaaatt aatttttatt cattttatac ttgaaatact tgtgtaaaaa gttcataata
     3901 tccaataata tgaataaagt tgctttttgt taagcaagag cagttgcaaa atagaaggga
     3961 atcttttgca agtgatttgt tcaattttat aagtgagggt gatggaaatg gaaaagaaat
     4021 cgacgggtat tagcaaatgg ggactggttg ctctggttgt cagttcgtca atcggaagcg
     4081 gtgtgtttgg gatcacaagt gatctggcta gttctgcagc tccagggcca gcgatccttt
     4141 catggataat cgttgggatt ggtattttag ctttggtatt atcgctgaat catttaggag
     4201 aaaagcgtcc agatctagat ggtggtattt tcggttacgc ggaagcttca tttggtaaat
     4261 taggtggctt catcagtggt tggggttact ggttgtctgc gtggctggga aatgttgctt
     4321 ttgcaacgat gctgatgagc gccatgggag aatttttccc gatattcaaa ggtggacaaa
     4381 atgttccttc tattttattt gcaagtattt ttatttgggg tctgacttta ttagtaaaca
     4441 atggagttga aggagcgagc gtcatcaata caatcgtgac gatctgtaaa ttagtgccat
     4501 tattcctatt tttaatattt gtatttatcg catttaaagt gaatatattc actgctgatt
     4561 tttggggtaa cgtatctgat aatctagtga atggaacagg gcagcaagga acgatctggt
     4621 tgcagattaa aggttgtttg atggttatga tgtgggtctt tgtagggatc gaaggcgcaa
     4681 gtgttttagc caaccgcgcg aagaaacgtt cagaagccca gcaagcatcg atcattggtc
     4741 tgctttgttt attgtttatc tatatccttg cttcgatctt gccatacggc gtcttatctc
     4801 aagaagagtt agcaactatt tcacaacctg ctatggcaaa tattttaaaa ggaatcgtgg
     4861 gaaattgggg agcagcgctg atcaatatcg gcttgattat ttcgatcatt ggttcttggt
     4921 tgtcttggac gatgctccca gcagaaacaa cgatgttgat ggctcgagac ggtctgttgc
     4981 cgaaaggttg gggaaaaacg aataaaaaaa atgccccaac ttattcgtta gttgttactg
     5041 caatcttgac taatctattc ttattgactt tcttagttac tgattatgcc tatcaatttg
     5101 cctattcttt atgtacagcg gcaattttga tttgttatct acttgtcggg atctatcaag
     5161 tccagttttc tttaaaagaa ggaatcaaaa aacaagtagt gatcggtggg attgctgtag
     5221 tttttgaatt gatgggcatc attttagcag gattttctta tgtcctttta tgtagtattg
     5281 cctatcttcc tggtttttgc ttctactgga tcgcttgtcg tgaaaccaaa catcaaatta
     5341 cgggaaaaga aaaagtaatg attgggctga tttgtgtggc cgcactgctt tcaatcgggt
     5401 tgcttgctgt aggctggatc aagatataag ggggaaagaa acatgaaaat cgcaaatttc
     5461 ggtgtagaag aatggctaaa taaatgggaa aagaaagcgg tatatgatat tgcacaaagc
     5521 tcgatcgaag cattgacatt agacgaactc gttggcttag atgggactag cgtaagtaaa
     5581 tttttcgaaa aacgcaaaaa cgaaccattg gactatggct ggatcgaagg atcagatgtt
     5641 ttcaaacaag aagtcgcttc tctttatcaa accgtcgatc cggaaaacat cttacaaacg
     5701 aatggagcga ctggtgctaa tcttttagct ctatacgcgt tagtggaagc aggagaccat
     5761 gttgtttcga tgcttcctac ttatcagcaa ttatatgaca tccccaaatc tttaggtgcc
     5821 gctgttgact ttgttcattt gaaggaagaa gaagattggc agtttgattt agaacagcta
     5881 gaagccttag tcaaacctga aacaaaaatc atctgtttga atagtgcaaa caatcctaca
     5941 ggcacattgc tagatcggaa aactttagaa aaaatcgcag aaattgctcg tacagtagat
     6001 gcatatgtat tgatagatga agtatatgcg cctttaacgg ataaaggtga attcttgtcg
     6061 atcgtagatg tctatgaaaa aggtatagca accaattccc tatccaaaac ctactccatt
     6121 ccaggtattc gtattggctg gacagctact ggacctgagt tggcagaagt ttttcgaaag
     6181 tatcgagact acacgatgat ttgcggcgga gtcctgtcag atgatcttgc tgtacatgca
     6241 ttgaagaata aagaaaaaat attggcgcga aacaaaaaga tcattacaga gaaccttgtg
     6301 attttaaaac aatgggtagc caatgaaccg aaagttgaac ttgtcgctcc caattacgtc
     6361 tctacttctt tcattaaatt agccatcgaa gaagatgacc agacattttg tatcaattta
     6421 ttagaagaaa caggagtact attagtacca ggaagtgcct ttgatttacc gaagcatgcc
     6481 cgactaggtt actgttgtaa aaaagaaacc ttggaaaagg gattattcct cttatctgag
     6541 ttcttgaaaa aacaggccta aacaaaaaca ctgcaaggac agtctgtgac aactgttcaa
     6601 gcagtgtttt tcatttacgc ttattgtcta tgagaaggag agtgccctga agctatggag
     6661 actcctgaag ctttttcttc tgcagtttct tgctgatgga tctcttgtgt attgtgtcgt
     6721 acaagcaaga agttgaccaa aacaagaaga gaagtcgaaa gaaagacgcc gcgataacca
     6781 aatgcactgg aaacactgga gccgatcata ggccctacta cgttaccagt tgcttggaaa
     6841 gattggttat aactgaagat ccgtccagca gcttgatgag gcgaatattt tgtgatcaat
     6901 gcttggacag cagggagcag acaagcatca gagataccaa tcaaaaaacg taaggcagct
     6961 aattgccaaa cattcgtgac gaatgccatt gggatataaa ccagaatcgc aaaacccaaa
     7021 ccaatcgcaa gtattctttc actcccaatc ttgtcaccta agcgaccaaa tcttggagcg
     7081 gctatcaaag tggcaatccc aggtatcgaa gcaatgattc cgctgatcaa cgtaacattc
     7141 ccatgtccat gcagcaattg gcgaatgtac agactgatga ttggactaat ggaattgttg
     7201 gatgcttgga tgatcatcgt tgtgacgaac ataccgatga cgacgtgtgg atatttcaag
     7261 tctttgaaaa tctgtttggc agaaaccatg ttttcttttt tcactgggac aaatttttca
     7321 tggacgaaaa acagactcaa caagaagacg aaaaataaga tgatccctgt aatgaaaaag
     7381 gtagggcgat agccaaacgc tgaagcagag atcccgccaa gaagcggtcc aagtaatgtt
     7441 cctgtaacag aaccagttgc taaggttcca agaacttgac cacttttttc ttttggcgtc
     7501 ccagttgcga caagggcagt ggcattactg atataaccag aaaaaatccc ttgaagcaga
     7561 cgcaatgcga ctaattgata cacactagtc acgcaaccca tcaaagaaat gacgattgcc
     7621 attcctaatg aagcacgcaa cagcatcaat tttcttcctt tttggtcggc taattttccc
     7681 caccaaggag agataagcgt agtcaccaag aaagtagatg aaaaagtcaa tccgctccaa
     7741 aagttcaatt gtgacgtact ataattacct aacgtatcta tgtaaagtga cataaaaggc
     7801 atgactaaac taaatccgat accagccata aatgttccaa accataagac aaacaagttt
     7861 tgttcccaag tttctctttt cctaaataat ttttccttta ttcttgcagc caattagact
     7921 ccttctttct gaaaattttc atctacagct ctttattata aactacaata tgtagtttat
     7981 caatgaaagt ctcttttttc tctttctttt tcgtggtaaa ctgaaagagt gaaggattta
     8041 ttttatgaga ggaaacatgg aaatgaaaaa aagaaatctg tctcaagaga aaatcatcga
     8101 ctgctttcga gaactagcag aagagatgga tgttcagcaa ataacctttc agcatttagc
     8161 caaacgcttg gatatcaagg ctccctcgct gtataaccat ttcaaaaata ttcgtgatgt
     8221 caaaacagcg ctaacagcga aacttctgca ggaacttaat gaccagctgc gacgggctct
     8281 tgtgggaaaa agtggtcagg aagcactgca gatctatgca gaaacttatc gatcctttgc
     8341 tttttcaaat caagcagtgt atgagttact gatcagtgta ccgcatacca atgaagctgt
     8401 tttattagat ggaatccatg aaacgaatca gatcattttg cagatatttg aggcttttca
     8461 attaacgaaa gaagaaaaag ttcatcgaag cagggaactt cgtagtatga tccacggtta
     8521 tctttcctta cgttttttag gatactttac aaaagaacca acaatcgatc cagaagacag
     8581 ctatttttgg atgatcaata actttatcgc tactttgcct taaacaatca gattcatttg
     8641 taacaatttt aagatgttca ttaaatgacc cttttataag agaaaattct tgcaactttc
     8701 ttgaaaagtg actaagatga gtctagaaaa gctgaaagaa ggagcacgag tatgtatcaa
     8761 gtaattacca tgttcgggga caatgagcct tggtggtttt ttgaagactg ggaagaagat
     8821 attgaagaag aagaaacatt cgagtctttc gaagaagcac gacaagcgta cgaaaaacaa
     8881 tggaacgaaa tccatcaaaa ttatgaatat atcaatgcaa aaagtaactt tctcagcgcc
     8941 ttttggaatg acggagatga gcgctggtgt gaagagtgtg acgatgattt acagcaatac
     9001 aagggacttg cgttgttgaa aaatcatcag cccatcacag tagaaagcag aaaggaattt
     9061 tatgaaacaa ctaattctag cggaaaaacc aagcgttgcg aaagacctaa gcaaggtgct
     9121 tggtgctaat caaaagcaca aaaattatta tgaaggaccc aaggtgatcg ttacttgggc
     9181 ccttggtcat ttattaggat tgaaaatgcc ggaagatctg aataaggaat ggcagagctg
     9241 gcaaatggac acgttgccaa tgatccctaa aaaactagga atcaaaccac ttcctaaaac
     9301 aggacatcaa ttaaaagcaa tcaagcagct ggctcagcga aaagatgtct cagaagcagt
     9361 cattgcgaca gatgccggaa gagaagggga acttgttgct cgctggatct tagagtatgt
     9421 gcatttcaat aaaccagtga agcggttatg gatctcttct caaacagata aagcaatcaa
     9481 aactggattc aagcaactaa gacctgcgaa agaatatgat gcattgtatg aatctgctct
     9541 tgcccgggca aaagcggact ggctggtagg attgaatgtg accagagcct taactgtgaa
     9601 gtacgcggat aatttatcag caggccgtgt gcagacaccg acactagcat tagtgagaga
     9661 ccaagaaaag aaaatcgagc agttccgtcc acaaacgtat ttcacgatca cgttgcaagc
     9721 tggaaaagaa caagccaaaa tgcttcaaaa aaatcaattt gctttaaaaa cacaagaaga
     9781 agcacaaaaa cttgttcaat tgatcagtac aagcaaagga actgtcacag aagtccaaga
     9841 aaaattaaaa acggaatctg cgccattgcc ttatgattta acagagatcc aaagagaagc
     9901 caatcaacgc tatggctatt ctgcaaagaa aacattaagt cttgtacaaa gtttgtatga
     9961 gacccataag atcgttactt atccgcgtac agacagcaaa tatctgacaa atgacatgaa
    10021 aagtacgatg aaagaacgat tgcaagcagt cagcgacttt gctcctgagg tcaagcagta
    10081 tttgaaaaac ggagccatcg tccaacagac aaaagtattc caagacagta aagtgaccga
    10141 tcaccatgca cttttgccaa ctgaaaacag agcacgttat gaaaaattaa gtaatgaaga
    10201 gcgaaaaatc tatcaaatga ttgtctctcg ttttttagga ctgtttgctc aaccccataa
    10261 agtcagccaa acaaaagtta cggttgaatt tggtaaagaa cgattcattt ttcggcaaaa
    10321 tcgtgtcgtc caagcaggat ggaaaggcga aactgaaact gagacggaaa cagtcaaatg
    10381 ggaaaaagga atgagaatca atcccgattt cgccatcaaa aaagaactat ctgcaccgcc
    10441 taaaccattg tcagaagcaa gcttgcttgg tcaaatggaa aaatacagtc tgggaacccc
    10501 agctacgcgt gcagagatta tcgagaaact aatcaaatct gaactgatgg aacgtacccc
    10561 tcaaggactg caagtatctc ctaaaggtaa gcaattatta gtattggtaa atccctcttt
    10621 agtaacacca gatttgacag aaagctggga aaaagacctt gaagctattg ctgcaggaaa
    10681 gaaacaagca ggaaccttct tgaatggaat cgaaaaagaa acaaaacgct tggtgaatga
    10741 gatcaaatca agcaaacaag aatatcaaga tttctcgatt acccaaaaga aatgcccaga
    10801 atgtggggcc aatctgcgcg agaaaaacac acgtgacggc aaaatctatg tctgcaccaa
    10861 ttcagattgc aactaccgca gaagaaaaga tccaaaagtc tctaatcatc gatgcccgca
    10921 atgtcaccgg aaaatggaaa tcatcgaagg taaaaacggt gcttatttcc gttgtaaata
    10981 cgatggaacg acagaaaaat tgcttgataa aaaagagcag aagaagaaaa tgaccaaaca
    11041 tgaagaacga cgcttgctga aaaaatatgc acaagcagaa gaaccagaag aaagcccatt
    11101 agcagcagca ctaaaagcag caatgaagaa gtaataagat atgaaagagg ttgtgataga
    11161 agtggtcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    11221 aatttcggct tatttccgaa gaagctactt ctggaacacc atttattcgg aaaaaaggac
    11281 tgtgacaaga cttttgtcac agtccttttt catttaaaaa aatgtttact tcatttgacg
    11341 tatattattg gtgaggcttc aattcatttg gcttcacttt ttctgtttct cttatgatat
    11401 aaacaggacg ctgtttcgtt tctaagaaga ttttcccaat atatttcccg atgattccaa
    11461 gacataacaa ttggattcca ccaatgaaca ggaagatcga gaccatggaa ggccatcctg
    11521 atgtaggatc accaaacaac aatgcccgca aaataatgaa caaaattgca ataccagaac
    11581 cgacacaaga caaagcgccg ataaatgaag caatcgttaa cggtgcatcc gagaagttca
    11641 cgataccatc aatcgagtag ttgaataatt tccagaagga ccaagatgtt tcgccagcca
    11701 cacgttcgcg gttttcataa gaaatatatt ctgttttaaa tccgacccag ctaaataatc
    11761 cttttgaaaa tcggttgtat tctgttaatt ctaggacggc atccaccatt tgacgagtca
    11821 tcaagcggaa atcacgagcg ccatcgacca tttctgtatc gctgatttta ttgatcaact
    11881 ggtagaattt tttcgcaaag aaactgcgta tcgcaggctc gccttcacga gtcgaacgcc
    11941 gggtgccgac acaatcgata tcagaagtag tgatccggtt gatcatctca ggaagcagtt
    12001 cgggcggatc ttgcagatca acatccatca cggtcactaa atctcctgta gaagcttgca
    12061 atccagcgta cagagccgct tctttcccaa aatttctgga aaatgagaca aagcggacat
    12121 aaggataagc tgaggctagc ttcttgattg ctgctaacgt accgtctttt gatccgtcgt
    12181 caataaacaa atattcgaac tcatgggcac ctttgaattt ttcaacctca tcaaaaaaaa
    12241 gaggaacagt tttttcttca ttaaaacatg ggacaacaat tgatactttc atcgtttcta
    12301 tcctttcaat cgtatgttta tatatggttt acattcaaaa tttaataaac cttaaataac
    12361 acctttaaaa tttgatttta cgtaaaagaa caggtattct ttttgtaaac taacgtattt
    12421 agtttaccat gtttcctcgg tttatcaaac ttttaagaac aatttttatt agttggcaag
    12481 tagttttgga tagagaaaaa ggaggaagtt ccagtctatt gactaggagc aaacaaaatg
    12541 aaaatacaca cacctatcgg ctttttatgg ttagatgcag atgaatccgg attgactgct
    12601 gtatcttttc agccaatttc agaaagcgaa tcagcgaatc agcgaatttt aacgcgcaca
    12661 caagaacagt ttgaagaata ttttgcggga aaaagaaaag aatttgatgt tcctttatct
    12721 ataaagagag gtacgccttt tcagcaaaat gtctggaaag ctttgtcgac cattccttat
    12781 ggagaaatcc gaacgtataa agaagtagcc gttgctgttg atagtccgaa agctgtgcga
    12841 gcgattgggc aggcaaatcg aaccaatcca ttgccaatcg ttattccttg tcatcgagtg
    12901 atcgggcaaa atggaaaact gacaggatat atgggaaacg caatagatgg ggttgaaatc
    12961 aaacgccaat tgttgttgtt agaaggatat ctgcaaaatt aaaaacagtt gtgattcgga
    13021 cattgttcca tgcgagaatc acaactgttt tttcatgttc attctaaact ttgatcagcc
    13081 aaataaatag taaagccatc actgtttgga gaagcccgac agctaatcca taagataaga
    13141 agcgtgtacc ttctttcatg aattttgcaa agtctaaacg cagaccaatt gccgctaaag
    13201 cagtgatctc aaaccagctg ctgataaaat gtgccgattg gtcaataact acaggtaaag
    13261 aaaacaagct gttgaccaaa caaaagatca caaaccctaa tacataccaa ggaagcggta
    13321 attttcgttt tttctgcgta tcagttggcg tttcaaacag agctggttgt ttcttgtgca
    13381 tgaatttttc aaacagatag acaacaacaa cgagcatcat aatacgtaga atcttaaata
    13441 gcatggcgaa ttcgacaaca gaattgtcaa gtaagcttgc cccagcgact acttgaccaa
    13501 cagattggag tgtaccaccc aaaagtgcac ttttttcaat gagatttcca ccaaacaact
    13561 gtaatccaag aaaagggaga gtcaacatca tcacagtacc cagtagattg actagtgtga
    13621 tgatctgtcc tttttcttcc tctttcgcat ggatggaagg ggcgattgaa gcaatagcag
    13681 aagaaccaca aacagcgttt cctccagcca tcatcaaaga agaagtatcg ctgaattgca
    13741 gttttttacc aatcaagtaa gcaccgatga tagtgactga catttgcagt aacacaaaaa
    13801 caattccttt tacaccaatt tgagcaatcg tttgagctgt gacagttgca cctagtaaaa
    13861 aaacagagaa ttctaatagt ttgctttcag caaattttgt gccgattgct agaacaggct
    13921 gtttaaaaaa agtatttcct aataaaatac ccaataaaat agcaatagtc gctccaccta
    13981 actgtggcag ataaatagca agaagcctgc tgaaggcagc gatggcaaaa gaaagcagta
    14041 aaccgggtaa gatttcgcgg accgctgaat cctttacaga ttttattgga tgtgacaatg
    14101 atttcatttg atcggttcct ttcgtcaata aatactaaaa gcatactcga acatagtagc
    14161 actaaaccag tattttgaca aaagaacaag aggtcgtgac aaaagtcttg tacgacctct
    14221 tgtttcgaat aaacggtgtt ccagaagtag cttctacgga aataagccga aatttcacaa
    14281 aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctgtc
    14341 acaacctctt attttgatta gaacttgcga aaataaaccg gttaaagtgt gtctagcgca
    14401 agagatttgc tataatagac gataagggta aaatgtaacg agaaggggtt gaaaaatgtg
    14461 tatcaaattg taacagattc ttgttgtgat ctgccatacc atgtattagc aaaagaaaaa
    14521 gttgattttt tatcaatgaa tctccaattg aatgatcaag agttagtaga tgacctcgga
    14581 aagacattca attatgaccg ctacatgaat cagctgaaaa atggtgcgat gcctacgaca
    14641 tcacaaatca atgttggccg atatttagaa tttttccgtt cttatgcgga aaaacagatt
    14701 cctgttttat atgtggcttt ttccagtgga ttaagcggct cttacagtag tgcgcttcaa
    14761 gcggttgaat tgctgaagga agaatatgaa gaccctgaga tctatgtttt tgatacaaaa
    14821 gctgcaagta ttggggaagg gcttttagta ttggaagctg taagattgaa aaaagaaggt
    14881 aaaagcatcg aagaaaccct agagtggctg gaaagtaatt atttgaaagt ccattcatgg
    14941 gtaacagtag acgatctgaa atatctagaa cgaggcggta ggatttccaa agcaagtgct
    15001 actcttggca gtctattgag tgtaaagcct attattacgg tggataaaga aggaaggctg
    15061 acgaatgttg acaaagttcg aggaagaaac aagtcgatcc agacaatcgt caatgagacg
    15121 gtaaaaacaa tcgttgcacc acttgatcaa atcgtttatg tggcttacgc tggggatcta
    15181 gagagcgcgg agaaggcaaa acggcttttg gaagagcaga taaagatgaa agatgtgaag
    15241 ctttatccct taggaccaac gatcgccagt cacacaggct atggctgtat cgcgattttt
    15301 tcgatgggaa tcagtcgata aaagcagtaa ctcattctgt aaaaaagccc agaaattgat
    15361 tgtaacaaaa acagcgactc ttaaaagata gatgagcatc ctatctctta gaggtcgctg
    15421 tttttttatt ttttaagcta aataggccat tatgcccata actcgttcaa ttgtttttga
    15481 atatctgcat tttccaaaaa ttcatcatat gtcgtatcag ctcgatcgat cacaccttta
    15541 tccgaaacgg caatgatgcg gtttgccaat gtttggataa attgatggtc atgtgacgca
    15601 aataagattg agccagtaaa tgccatcaag ccgtcattca atgctgtgat tgattctaag
    15661 tctaaatggt tcgttggatc atctaataca agtacgttag ctttcgaaag catcattttt
    15721 gaaagcatgc aacggacttt ctctccacca gataaaacat tcactggttt caatacttct
    15781 tcaccagaaa acaacattct tcctaagaag cttcttaaga aggtgttatc atcttcttct
    15841 ttacttgcat attgacgcaa ccagtctaaa atcgttagat cagtatcgaa ttctttcgtt
    15901 gtatcctttg gtaaataaga attacttgtt gtcacacccc aacgtactgt tccagtatct
    15961 ggcgtaattt cacccatgat aactttgaat agcgttgttg ttgttatatc attttctgcg
    16021 acgaaagcga ccttgtcttc tttgctcaaa ttaaaagata tatcatctaa aattttcttt
    16081 ccatcaatcg ttacacttac attttctacc tgcaataagt cgtttccaat ctcacgttca
    16141 ggtttgaatc caacgaatgg ataacgtcta gaagaaggtt ggatatcgtc taaagtaatt
    16201 ttttcaagca tttttttacg tgaagttgct tgttttgatt ttgatgcatt cgcactaaag
    16261 cgggcaataa attcttggag ttctttgatt tgttcttctt ttttggcatt tgcgtttgct
    16321 tgaagtttag ccgctagttg actagattcc aaccaaaaat cataatttcc tacataaagt
    16381 ttgattttac caaaatctag atccgccata tgtgtacata ctttgttcaa aaagtgacgg
    16441 tcatgggata caacgatcac agtattttca aaattgatca aaaattcttc cagccaattg
    16501 atcgattgga tatccagacc gtttgttggt tcgtctagca aaagaacatc aggtttacca
    16561 aatagtgttt gtgctaataa cacttttacc ttttgtccac cagttaattc actcatttgc
    16621 tgatggtgca attcttcagg gatgttcaga ccttgcagta aaacagctgc ttctggttca
    16681 gcttcccatc cattaagctc agcaaattct ccttctaatt cagctgctcg aataccatct
    16741 tcgtcagtga aatcctcttt catgtaaatc gcatcttttt ctttcagcac ttcatacaga
    16801 cgcttgtgtc ccatgatgac tgtttcaagt acagtatagt cttcatagtc gtaatggttc
    16861 tgtttcaata cggccatccg ttcatctggt cccatagtaa cggaacctgt ggaaggctgc
    16921 agatcccctg ataacacttt aaggaacgtt gatttacctg caccatttgc accaatcaag
    16981 ccatagcagt ttccgggggt aaatttaata ttcacatcat caaatagttt gcggtctgaa
    17041 aagtgcaaac tcacatcatt tacagtaatc aatttttttc ctcactttta tcattattag
    17101 ttttgacaag tcttatagaa ttatataggg gaaaggtaaa agattcaagg caatcgaaca
    17161 ttctttgtgt ttcacgtgaa acagaatgaa aaaaataaaa ataaaagcgg agcgaaaata
    17221 gctactatag gatttattag tttgcaacag cataattttt gacgttattt ataagaggga
    17281 gtactataaa actgtaaaag aaatgaaaga aggattgaaa atggatgaaa aaagaaccat
    17341 cgactggggc tcattgattt tgggaatttt atttgtactt gtctcattgt tatcatttca
    17401 agaccctgtc ggaaatttag ttgcaattgt agtagtcttt gcgatttttg cctttattaa
    17461 agggatattt gaactgtttg tacggaacag attaaaagag ctaacaggat ataaaggaaa
    17521 gatgccctta ataatcggga tcatcgatat tctagttggc gtctttttct tattcaatat
    17581 tggtgcagga gtcgcagcat tgccttttgt ttttgcagta tggtttattg ctgattcagt
    17641 tttagcgtta tttactgctg acttggcaag aggagtaagt gaaggatatt attggtttac
    17701 gatcatcgtc aatattttag gaattttatt aggaattttc ttattgttta atccaatttc
    17761 ttctgcgttg acattaagct ttttagttgg attctatttt atgttatttg gtatcacaca
    17821 tatcgtctat gcatttagat aagtaaaatc aaacaaataa aaaagccggc ttccgattac
    17881 agttttactg ttgtaattgg aagccggctt ttttatcatg gacaaatatg cacgaggtga
    17941 tttttaggtg tcgagacaaa tttatgcaaa aatgtgagaa gctatataca caaattttat
    18001 cttaatactc gaagcatagc gcagttgtca caacctcttt tcatataatt cattcaatac
    18061 tggcaaatca tggacttcat atttttcttt gaatgcttgg atttcttctt cagaatattt
    18121 tcttggatct aaatgcagat cttctactgg caatgggcgc ttgttattga tcttcacatc
    18181 gattactaca ggacgatctg attttgcagc tgcatcaaag gcaaatcgaa gttgcgagta
    18241 ttctgtgact gtaaagctat ttgccccgag cgcttctcca actttaccga aatctgcatc
    18301 ttctaaaaat acaccaaatt tcttttgttc cgtatcttct tgttctgctt cgatgaaacc
    18361 aaaagaatca ttcgaaaaga caacattaat aattggaagc tgatatttga cttgtgtaat
    18421 gatatcttgc atgaccatag caaacccgcc atcaccactt aaggtaaata cttgtttttc
    18481 tgggtagctt agttgtgcgg caattcctcc tggcacacca ttacccatag ttgcgaacca
    18541 tccagatgtc gtatgttttt gtttaccgtt catattcaat aaacgaatag aatggatagt
    18601 cgtgttaccg acatctgtga cgaaaatcgc atcgtcttca gcaatacgat tgatttcttt
    18661 atagacagcc tctggacgaa tcggttcttc ctctcgatct tcaaaagatt tcaaccaatt
    18721 tacccatttt tctttatttt cttgatttgc taggtaccag ctatcagcag ggcgagcatc
    18781 ccctaactcg acaagtcttt gcaaagctgt gtttgcatca ccgaggatcg acaaagatgt
    18841 ttcatggcgt cgtccaaatt ttgacgcatc gatatcgatt tggataaatt cagcagttgg
    18901 attaaaaaat gcacgtccaa atgggaagtc gctaccgaca aataagatca gatcggcttc
    18961 tgccaatgct tcatttccgg gtttagtagc gacacgtcca gcaaaaccaa gaaagttttc
    19021 ataatcatca ggaataattc cttttgctag aacggaagcg acaacaggaa ctgaaaaatg
    19081 ttctgagaat tctttgattt gcggaaatcc gtttcgcgtt ccttgaccaa cataaagaac
    19141 tggttttttt gctttttcaa gataaggcaa agcagctagc aaatcttttt cttctggtaa
    19201 tatcacactt gttttgtgag tgtgagcggt ggagaaaggt tgttcgtcaa tttcttcaaa
    19261 accaagatca acagggatag tcacgatagc cacgccttta tgctcatagg cggctttaat
    19321 ggcttcatct actacatgcg gaagactttg tggtgtcatg actgtacgat tgtatacgct
    19381 aacatctgcg aaaataggat tttcatttag ttcctggaaa gagttatagt tcatggatgt
    19441 agaagcaact tgtcctaaga acgctactag aggcacatgg tccatctgtg catcatacaa
    19501 accattgatc aaatgtgtcg caccaggtcc tgcagagccg aagacagcgc cgactttccc
    19561 cgttaatttt gcatctgcag cggctgcgag tgcgccgact tcttcatgac gaacttgaat
    19621 gtagttgatt tcggtttttt cttgatatag cgcatccata attgaattga aagatcctcc
    19681 gggaatgccg taaatatggt cgatacccca gctttcaaga acttttacca ttgcgattcc
    19741 tgcattgatt tttgacatgg tgtttcactc ctttagcaat gatgatacat aaattttttg
    19801 tatctagagt atagaacttt ttatcgaaaa tcccaactaa ttcgtctttt cacaaaaatg
    19861 tagtcatgtt tttgggaata tgctagagta gtacatagat agattcaaga gaggggaatg
    19921 gcatggttga atggctattg agacttgaac cttggcagca agcacttgct gggacagctt
    19981 ttacttattt tatgacagct cttggtgccg ggttggtttt tttctttaaa gaaatcaaaa
    20041 aagaagtgct aaacttgatg ttaggatttg cttctggtgt catgattgct gcaagttttt
    20101 ggtctttgtt agatccggct attacaaaag cagaagaaaa tggagatatt gcttggctag
    20161 ttgtaagtat tgggtttggt ttaggcggtg tgtttttata tatggcagat aaaacactgc
    20221 cccatatgca ttttggacca caacatgaag cagaaggatt gcctacccat ttaaaacgga
    20281 cgatcttgct tgttttttct attacattgc ataacattcc tgaaggttta gccgtggggg
    20341 tagcattcgg tgcagcagca actgcggata atccgacagc cgctatatta gccgctgttt
    20401 cggtggcgct aggtattggt atccaaaact tcccagaagg agcagctgta tcaatcccgt
    20461 tgcgacaaga aggtctgagt cgtaaaaagg cctttgttta cggacaagct tctggaatcg
    20521 ttgaaccaat tgctggagtg attggagcgc tactagtaac gaaagtggag ctgctgcttc
    20581 cttatgcact tgcttttgcc gcaggagcaa tgatttatgt cgtggtggaa gaattgatcc
    20641 ctgaagcaca gcagacattg tccagcaaac gtcattttgc agtttttggc gtcatgagtg
    20701 gatttatcat tatgatgatt ttggatgtgg cattaggata aataaaaaaa atatatatga
    20761 tatatcaaat agggccgcga acttatatcg cgaccctatt tttttagtaa gttatatcat
    20821 aatgaagtga atggtatcat acattaataa tagttttttc tatgtgtatt tcctttgatc
    20881 cagcgaacaa gccagatgat aagtgcgatt gggattagaa ctttgaccgc aaaccataaa
    20941 agcccgctta ggacactgac aaaaagactg atgacgaagc tagctgcaat tagcactaaa
    21001 agaaccatga agatatttaa gaacatattt ttttgacgca tacaaaaccc tcctcttgtt
    21061 cattctttac tatagaaaaa gtataacagg gattgtttaa aaacacctcc aactgaagac
    21121 tgatttttac ttcatctact taaatgatca aacaattgct ttttctctgg tttatcatct
    21181 aaaatagagc ggaaaaggag gagaatatca tgcctaaaaa tcgtcttgaa gcatttacag
    21241 atgctgtaat cgcgattatc atgactattt tagttttaga acttcatgaa cctaacggag
    21301 acacgtggga ggcgctcgct gcattaggtt accgattttt tatctatatt gtcagtttta
    21361 tcattttagc gatttattgg aacaatcatc atcatttatt tcaagttgta cataagataa
    21421 atggacgggt cttatgggcg aacagtttct ttgtttttgc gctttcgctt tttccgtttg
    21481 caacttcatg gatcagtgag tacatcaata gcctggctcc agaaatgact tatggttttg
    21541 ttaccttgac cgctaatatt gcgtattata tcttggcaaa agaacttgtc aaagcaaata
    21601 aaggccacga gaaagcattg aaggcgatgt tgaataatta caagaaatcg tatctatcga
    21661 ttggtttgaa tgtgttagga attgtcttag ggtatttagt agcgccagta gccgtgctga
    21721 tcgcaaacat cgtgattcta gctgcttgga tcatcccaag tcgtaccatc gaatcacaat
    21781 atcggtaaaa tgatccgtgt ttaaaattta agagaaaaat atagaaaaaa taaataaacg
    21841 atcaaatatc cgagtgatac tagtattcaa agagggatgc agatggatca aaaaagaatt
    21901 catgtaggac tgattactag ttttgtcacg atgcttattt atttggatca aggtcatttg
    21961 acttggacag tagttggtgt cgtaggattt ttgacattat tcacagcaat aaaaaaataa
    22021 acatctttat gtaatctgat gttctatcta tttaagaaaa gcatgtttac agcagtatga
    22081 ttctgttgta aacgtgtttt tatttgtgga aaatttaatt tttagaaaag tgtatcgaaa
    22141 aatagatagt tagtttttcc acagtggata acttcgtaag agataaaaat aaaaaattag
    22201 tcaaagaagg gcgtatagat ttcttttgat aggagatata caacagaaat gaataaatat
    22261 taacgtgttt tcaaacagta taaaacggtg tggtttttgg aataaaaaaa taacatatct
    22321 cattttacac ttttgttatt ttatacttaa aaaataacga cacagaaaat accgtgtctc
    22381 agatttctct actacttcta aaaactactg tttttttcct ttgaaatgcc ttcaaaaagt
    22441 aggatcaggc agaatatact ggcagaatca aggagaaggg agcctaaata aggagaaaaa
    22501 ccatagaaaa tataaagtaa ttacttataa aacaagcttt ttatagaaaa ataatatacc
    22561 tgagtagggt ataaattaag acggaattcc tttctagatt gagatattga ctaggaagaa
    22621 tgttaagatt ttaagccttt aagctaaaaa aaaacccttt ttgttaaata aggggaatat
    22681 agcctgtcag tttcaaactg caagtcgtcc tgctgcaata atatagtaga ttagagatga
    22741 aaatagattt ttttaaacca atgactgaat aaaaagaccg tcatagatac acattggaat
    22801 gtgtctatta cggtcttttt ttacgaacga ttttattgaa gacggacagt tcgggtcaaa
    22861 aataaaaaag ctacgatcag aaataagaat aaaatgtatc ccaaaataat ggctagacgg
    22921 tctaacttat ttgtcgatag atgtttgttt agaagttgat aggcccataa acctatcagt
    22981 cctcctgttg tattagttat caaatcagtg atgtccgcta cgccaatacc gaataggaat
    23041 tgaatcaatt ctgccaagaa gcttacaagg aaaataatcc ctaatttttt agaaaaagaa
    23101 agtctcttga aattgacatt caaaagcacc ccaaaaggaa aaaagacaat caagttataa
    23161 gtggtctcac gtaataccgt cgtattttca aaagaaaaag tagaaaaagg gacgagattg
    23221 acattgctgt aagaataagt gagaaattgt ggaagattaa atgatagctt gaataaaatc
    23281 aaccataata gaaagaataa ataaatccct aataaaaatt ttgatacata ccgcatagac
    23341 atctcctcca tttaaaaagt ataccatcga aaaccgctga aaaatcataa aaaagtatga
    23401 agtttaagga ccaacgtagt tatcgtacga aaattagaag cttttttttg ttgggaatcc
    23461 ggtttcttta acaaagctaa gatagcgagt tgcttggaaa agttgctatc ttttttctgt
    23521 caaaacgact cggaatgttc gttttttgtt ctaatttctc caagttgttt actttttgat
    23581 actaagttta tgaaaaatca agaaatagtt gttatactag gtaatgctga caaacgggat
    23641 tggcatttca tcttggttca gctttttttc caattactag gaaggaggga tagattgttc
    23701 aagacttatt ttcgtttcaa taaaatacaa gataatcctt ttcgtatcat gaatctgatg
    23761 ctacttatct ttattgtatt atggttaggt tacataaatc atgatttttt gatttcaagt
    23821 ttttcatcat tagggatctt tacttttttc tattatcaaa acataccgat gaaatatttg
    23881 ctgaaacgta tgtttctgat cggttgcgga ctgttggttg ctttcgtttt ggggatctta
    23941 tcgacttatg ttttatggct tgaaccattt gccgtagctt tggttgcttt ttccagcaga
    24001 ttcgtgttgc gattattcca tatttctaaa ccaggtggct tgttttttgc gatgctttct
    24061 gccatgggaa cgagcatgca gctgccaatc gctcaattac cgatcgtctc actttatttt
    24121 tttatgggtg tcgtttttgc cctgattgcg gcagtgatta caaaattact ggattcacgg
    24181 cctgagcaga caattgaaaa agcaacttta aaagaaagat ttcacgaaga gcctttagtg
    24241 atcattgatt ctgttttcta cagcgctgcc ttatttcttt ccgtttatgt cagtcacggc
    24301 ttgaatctgc ataatcctta ttggttgacc ttgtcatgtg catcgatttt gctagcagaa
    24361 aacttggatg cgatgaagca tcgacaagtc caatatctga ttggatcgat gggtgggtta
    24421 tgtgtgtcgg cattcttgtc atttgtgccg tttacgcagt tgcagaccat ttttctgatc
    24481 acttttctat atggaatcgc acaatttcta gttgctagaa attatgcagt tgctaatatt
    24541 tttcttaatc cgatggcgtt gatgctatct acacttattc gtggagcata cttgatttct
    24601 ttgatcgaat atcgtttttt agggatcgtc ataggaagtt tcatcggttt aggtgttgcg
    24661 tgggtaatga cggttggttt gcaacattat ttaacggttg ttcgtcaaaa attagaatag
    24721 aaggacaaat atatgtgcca aaagaggtta tgacaagatt tttgtcacaa cctctaattc
    24781 aaggtgttca aaagccagat tctcagcatt aagtcaaatt cacacaaaaa tgtggaaagg
    24841 aactttcaaa aaaattttat atttattcta tcaaaaaatt ttgtttatga tggtaactga
    24901 ataaactttc ctgagatggt caatgctttc cttgaaagtc ttacagatat tctttcttca
    24961 tagaaagttt aagttttata gttagtgtac aataaaagta tgtttatacc atatggtgtc
    25021 cggataatca gtcatttaaa caatgaggtt caaagatatg aaaaggggaa atttgatgaa
    25081 gaaaataagc aatgaaatcc aaattctggc agaaacttta actcttccca aagtcgttat
    25141 agagcaaatg attgtacaag caaaaaacat ccttgatgag gtgtttgagc aatatagtca
    25201 tgaactaatg gatcctaata aagcaaaggc gactgcaaag aaaatagaca aagtctatga
    25261 agaggaacct tttgcagcat tagctattta tctatatgca gcttctcaaa gttggaaaaa
    25321 aatttatcaa ccattgaaga ttccaagaac tatctatttg gcaacaatga atgcttttac
    25381 tagatttatt catgaacatt tccaagtaac aggaagctat cgctttgacc gtggtttttg
    25441 gatttggcat tacactagtg gcttgatctt tcggattggt gaattggagt acgaaatgac
    25501 atattttccg aaagggtaca cggatctaag attagaagga aagcagtgcc tttctcttca
    25561 tattccttcc gatgcagatt tatctcagaa taagataaga gatagttacg aaagtgccat
    25621 tcgttttttt gaactttatt ttcctgatta ccattaccaa gtgatgtaca cagatacttg
    25681 gttactatca cccaatctta ctaaatggtt gaaaaaagag tcaaaaatct gtctttttgc
    25741 agccgattat cgattattat cagttgatga acaggacgat agtggagtcc cttggatctt
    25801 tggacgagta gatgcccaaa tacatgatta tccagaaagt acgagtttac agcgacaagc
    25861 aaaagagcaa ttgcttgcag gcgagcacat tggctctggc ttaggtattc ttacttcata
    25921 agtttagtac ttcgaaatag tttaaaagag cgtgagacaa aagtaaagaa cacttttgtc
    25981 tcacgctcta aacacgtata aacggcggaa gcagaagcaa ctccttcgga aataagctga
    26041 aattcacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt ggagttaaac
    26101 gcttctgtcc cagcctcttt tttagtgttc tatcgaacac acagtacgtt caataggtaa
    26161 actagctata acaatagata ttaaagttaa cggacaagag ggcctttttt aggtagctaa
    26221 aacgtaataa tgaaaattta tttccagaga taggagtcaa tcagctagtc tgatcgactg
    26281 ttttaacaag ttgactttca ttttaacttt tgataccatt taaaacacaa atcgtaattg
    26341 tttcgatttg tgttttttag catgactaca atcaaagaag aaagtgggtc gtccatatgg
    26401 aaaaaacaga tatctcaagt gcataccgcc gcttgaaaag cccaaatatc aaaacacgaa
    26461 aacgtgcgct gaagatcatc aaagatgtga aacggaatag tgggaaaagg taagaagaag
    26521 gaaacttaat taaaaaaata gccgaaaaac gtttttcggc tattttcaat aatcggtttt
    26581 tgtgtaaggt ttccgaaaga actgctgttg attttccatt tttaacatat gataaattgg
    26641 tagaatcttt cttttcaaat tgataccatt ccctaattcc agttatatag ttaactacag
    26701 aaccacaaag cacgtacgtt tataaaagta ttatctgtag ttatttttca attccctttc
    26761 aacctcttat tttgaccagt agtattgatc actttacctt ttaacacctt tgttcgtttt
    26821 tgaaacattt ttagtcaaga atggaaacgt aactacataa tcaaaggttt tgcgaaatat
    26881 ttcatgaaat atttcatgta gaagcgagca atacggttgc aaataaccat tatcgtttaa
    26941 taagcagtta cgaaagctcc caatatctct aacgttcagc tcactccatt tcttttcttc
    27001 aaaaaaatca gtgattcgta tctctttcta tttgtcgaaa aagttaactt taaaaagtaa
    27061 cgccgaaaaa atatgcaaaa aaataaaaaa gcagttacta ttgctagaat aacatcaagg
    27121 aataataagt tttggttttc agataatagt cgataagttg tgtattggaa ttttagatac
    27181 agtaataaag tatttttcat gaaattttgt gagaatcact attacaagta tctttaataa
    27241 aattaatggt taaggaggaa cttaaagtag aatgtaaatg aatagttgta gtcagtcgat
    27301 tgtgatacta gactcaaagt tgcaggaata agtttttcaa tttgtgattg gaagataaaa
    27361 aatgaaaaag aagggctttt ttttatactt tgttgattgt agtgtttatt ttccctctgt
    27421 ttagccaagt aacattttct atgactcaac cagtttcaag tgaggaacat gataaccttt
    27481 ttcgtgctac agaagagttt aagaaatatc aggaaaatat tgttccggag actccattag
    27541 ctagtgagcc aattactgat attgatggaa accaaatagg atggtatata cagtacgaag
    27601 ttatggtggt aaataatgac tcaatagtaa aaaatacagt aggcttagag ctaacaccga
    27661 ttgaaaaggt gagttcattg ttaacgtata tttatactga tgaaagtgac gagactgaga
    27721 tcattttaac ggattactca aaaattgcta ctgataaaaa gtatatattg tggatgtaga
    27781 cactagtgaa atactttatg atatagaaat tgatgaaaat ttactagatg tagcacaaga
    27841 aatcgatcat caaaagcagg aagctattcg atatgctggt agaaaaggga aaagaaaata
    27901 tttattacgt gaatgtagca aaagttagag aagatgaaaa tgagtggaaa gaattcaaga
    27961 gtcgctactc aattaactcg actccgacat ttacagttta cagagaagga tcgatagaaa
    28021 aaactgtttt ttggactaaa gaaagtggaa tgtctttagc tgaagtagaa gagtttttgg
    28081 attatgttag tatgcaacag taagggagtt cttattatga aaaattttga cacatattat
    28141 gttattgttt tttcgatatc tttgctgtta atattactaa ctttaatgca gtataaagag
    28201 tatccagagt attttatttt aacttttgta tatttagtaa gtagctacac acttctatat
    28261 aaattcagaa gttcttctag agtttctaaa gtttgtttct taattagctt attgattcta
    28321 taatattttt gatttatata ggaaattaaa aatattatag aatcaacact cagagaacgg
    28381 gttggttttt aatagattta ataagtactg ataatgaaat ttctcctggt tgggaaaacg
    28441 aagttggtta ccctgtagaa gaattttttc aagattactt tgcggagtat gccgatagga
    28501 taatgagaga ttacgtaaca gataaaactc aagaagtcat caatcaatcc cagtcattaa
    28561 atgaattatc tgataaggcg aaagaagaag cagttgccat tatttcttca gggattagtg
    28621 actatattga tatgagtggt gaaatcaatt atggcgataa agacaaaatt gtacgcatga
    28681 taaaagcttt tatcaaaact tttgatgtga aggcagatct taagtttaat gatacttacc
    28741 tagtaggaaa attaattaat atccttggga cagagaaaga tacggaaacg tttatttcct
    28801 ctttgtctat gttgtttaca gataagcatt tttcaactca gcttggtgga gaggaactag
    28861 tggaaatctt ttaaccatta cgcccagcat taataaaagt atatcaaaaa agtactcctg
    28921 atgattttta agattggttt gagaaataat gtgaaaacac tatttttaat gttaattata
    28981 gatagagatg aacgcgtcat atggttaaaa gaatgaaaaa gtcaaagata cactgctata
    29041 tttcactaag gatatagcag tgtatctttc atgtcgtttc actggcagtt ttttataaaa
    29101 ggaggtgtgc atttcagttg cttttctttg gtagttagtt gaatcgttta agaattaacg
    29161 gatgtggcag acaggtgctc tgtccaaaaa ccagtaaaat ctttgatggt tccatcttta
    29221 tttagctgtg cctgttcaaa tactgttttg tttttaccga atggatcaat tgcaataatt
    29281 gttccgatcc ccgcacgttt ggcagcaagt aatcctgagt aagcatcttc gataacaaga
    29341 caatcagccg gagctaagct cagtttgtct gccgctttaa gaaaaatatc cggttgcggt
    29401 ttcccaggga aagagccgtc gtcatagaca atcttgtccc agtcaaacca gcgggctaaa
    29461 tcgaaaatat caaaataaaa agcaacattc tctttgacag tagccgtggc aatcgttaat
    29521 ggaatctgtt ggtcagtcag ctggtcaaga gtcgcttcaa gcccttttgt caactgaagc
    29581 tcgttttcat tttgcaagca aagcatacga taatagcttt ctttttcttc tgacaatacg
    29641 gcgatctcag aggcggtcaa atgctctgaa atgaaatgtt tcaaaatctc cgtattcgtc
    29701 cgaccatgaa tattttttaa aatatcctcg tctgataaac catcttttgt atgatcatgt
    29761 atcatatgca gccaagcagc ttcatgcaga tgagagtcca aaaacattgt tccgttgaaa
    29821 tcaaatataa tccctttcat agttacgctc ctatagtggt attttcggtt gaatcagcaa
    29881 tttgcagatc gacatattgt gcatagtgct cttgaacctc ccgcgagatc tggcgatccg
    29941 taatgagcag atcgaaatca tgcaaattat aaaacacata aaaatcatct cgattgaatt
    30001 ttttattatc aacgagtaga tatttcttac gggaattatt tagagcgatc tgctgtgctt
    30061 ctccttcttc ggtactgtaa gtagagatcg cttcgttgtg aacgccattt gaactgataa
    30121 atgcttttgt aaactttagt cttcttaaat tgtcgttggt gatcggtccg acaaatgcgc
    30181 cggtattttt ccggaaatca ccgccaatca gtagaatttc tggtccatct gcttgagcaa
    30241 gaatatcaaa aactggataa ttatttgtaa tgatccgaat tttcttgttc agtaaatgcg
    30301 tcgccaatag ttcgattgtt gtacctggtc ctaagaaaat cgtttctccc tcgtgaatca
    30361 aggaggctgc tgtggcagca atttcttttt tttcttgaat ctgcagagtg gctttttcag
    30421 tgtgagaaag ttcttgatct aaagtgtagg aaagactttg agcccctcca tggacacgca
    30481 gcagcttgcc cgatttttct aattcatcta aatctctgcg aacggtcata tcagaaacat
    30541 ctaattgatt gatgatttca ttaacggtaa ggattccttt tttattgacc atttctacga
    30601 tagtaagcaa tctttctttt ttcaacattg tcttcacctc taatttaagg ataatctatc
    30661 atataaacgg acaacagtca atctaattcg aacaaaagta aacatttagg gtggttttta
    30721 tgtttgttac aatagatttt attgctgttt tctggttttt atgattattt gtgtttgttt
    30781 ttgttgacaa atgttttgga tgcgtttaca atgcaatcag ttagaaagag cgaaaactga
    30841 ttatagattt gaggagagat gtacatgctt aaattaccag aagattttat ttttggcgga
    30901 gccacagcag cctatcaggt tgaaggagcc actaaggaag gtggcaaagg cgctgttgct
    30961 tgggatgact ttttagagga acaagggcga ttcagtcccg atccggcaag tgatttttat
    31021 catcaatatg caaaggacat tgaattgtgt gaacgttttg gtgtaaatgg tctaagatta
    31081 tccattgctt ggagccgcat ttttcctgat ggagctggga aaccaaatcc agaaggaatc
    31141 gctttttatc atcgtgtgtt cgaagaatgt aagaaaagaa acgtaacgcc gtttgtgacg
    31201 ttgcatcatt ttgatacccc taaacgtttg tttgaccatg gagatttttt gaatcgagaa
    31261 acaatagaag catttgtttc ctatgccatt ttttgttttc atgaattcaa agaagtcaag
    31321 gtgtggagta cctttaatga aatctatcca gttgcaacca atcaatattt gttaggtgtt
    31381 ttcccaccag gaatcaaata tgattttaca aagatcgtcg cctgtcttca taatatgatg
    31441 gttgctcatg cccgtgtagt gaactatttc aaagaaaacg aacttccggg tgaaattggt
    31501 gtagttcact cattagagac gaaatatgcg gcaacagacg cgccggaaga taagcatgct
    31561 gcctttttag acgatgcatt gtcgatccgt ttcttattag atgctaccta tttaggctat
    31621 tattcaactg aaaccttgac tgctcttgat gagatatgtg aagcaaatca ggccagctat
    31681 cattttcctg aagaagattt cgttgaattg aaaaaagcat ccacgcgtaa tgactactta
    31741 gggatcaatc actaccaatg ccattttgtc aaagcttacg atggagaaaa tgcaatccat
    31801 cataacggta cgggtgaaaa agggacctct gtttataaag tgaagggaat cggagaacgt
    31861 atttataaag aaggaattcc tcgaactgat tgggactggc tgatttatcc agaaggtcta
    31921 tatgacttgt tgctgcgaat taaaagcgat tacccacatt acaacaaaat ctatatcact
    31981 gagaacggga tggggtataa agatcagttt gaagatggca ttattatgga tcagccgaga
    32041 atcgactatt tgagagtcta tttggaatcc ttaagcaagg cgataacagc cggggtaaat
    32101 gtgaaggggt attttttgtg gtcgcttatg gatcttttct catggacaaa tgggtataac
    32161 aaaaggtatg gcttgtttta tgttgatttt gaaacccaga agcgttaccc aaaagaatca
    32221 gcttactggt acaagttggt cagtgaaacc aaaacgatta tttaataagt gataatggag
    32281 gaagaaaaga tgaataaaga ggaattacaa atgcttggtt ttgagatcgt tgcatattca
    32341 ggcgacgctc gcagtacatt attgaaatta ttgaaagaag taagacaagg aaactttgaa
    32401 catgttgatt cggcgttgaa agaagccgac gaaaatttaa cccttgcaca taattcgcaa
    32461 acaaaaattt tagcggaaga agcatctggg aaagagatgg agatgggatt catttttatc
    32521 catgggcagg atcatttaat gaccacttta ttgctgagag atctgattca agactttatt
    32581 gtactgtaca gacagaatca aggataggag aagaacgcta tggatggaat cattaaacaa
    32641 atcgaaaaag ggaaaccatt ttttgaaaaa atctcacgta atagttattt aggagccgtt
    32701 cgtgacggat ttttaacagc gatgccagct attttatttt caagcatttt cattttgatt
    32761 gctgcggttc ctgagatttt tggttaccaa tggcctgaat ctgtctccac atggctatgg
    32821 aaagtctata attattcgat gggtgttgta gggttactcg tttcagcaac cactgctcgc
    32881 tgcttggctg attcgatgaa tcgtcgctta ccgaaaaaca aagtaatcaa caccacctct
    32941 gtgatgctgg cctctgtggt aagtttcctg ttgttaagcg tctctccgat tgaaggtgga
    33001 ttttcaacgg aatatatggg gacaaaggga ctactggctt cctttgtcgc tgcattttta
    33061 acagttaatt tatataagtt ttgtgtgctg aaagatatta ccattaaaat gcctaaagaa
    33121 gttcctggaa cgatttcgca aaccttccga gatatttttc ctttctcttt ttcagtattt
    33181 gcggccgttt taattgattt aggcattcga caaatcttcc aaatctcatt tgcagaagca
    33241 atcattacgg tgctgcagcc gctatttaca gcagctgacg ggtacttagg aatcgctatt
    33301 atctggggag cgatggcgct gttttggttt gtcggtgttc atggaccgtc gatcgtggag
    33361 ccggcaattg cggcaattat ctatgccaat gtggaaacca atctgcaatt gttcaattcc
    33421 ggtgaacaag cttctaaagt actgacggtg ggtttaggga attttgtagg aactatggga
    33481 ggaactgggg caacattggt tgttcctttt atcttcctac tctttgctaa gtctaagcag
    33541 ttgaaggcag ttgggaaagc ttcctttatt ccagtaagtt tcgctgtcaa cgaaccattg
    33601 ctatttgcgg cacccatgat tttgaaccct tattttttta ttccattttt gttaacacct
    33661 gttgtaaacg tttgcctgtt caagtttttt gtcgatgtat tgggcatgaa cagctttatg
    33721 tacgttttgc cttgggcaac tccagcaccg atcggtttgg ttctaggaac gggcatgggt
    33781 atcttagctt ttgttctagc agcggttcta atcgtcgttg acttcctgat ctatctacca
    33841 ttttgcaaag catacgatgc tgtcttagta gagaaagaag cacaaaaagc agaggtgatc
    33901 gcaaccgagg aaggtgcttc tgccgatgta cgtgaggaag tacctgtggc agcgagtcaa
    33961 gttatcagcg atgcaaataa agaaatcaat gtgttggtac tttgtgctgg cgcagggaca
    34021 agtgcgatgt tagctaatgc tttaaaaaaa ggtgcatctg aatcgaaagc accgatcagt
    34081 gcaatggcgg gtgcttatgg ttctcattac gaaatgctgc aggattttga tatggtggtc
    34141 ttagcaccgc aagtcaattc ttactatgat gatatcaaag aagacaccga tcgcttagga
    34201 atcaagctgg ttgcgacaaa aggagcagaa tatatcggat taacgagaga tcctgaaaaa
    34261 gctgttcagt ttattctgaa ccaatttcaa taaaggagga gccataatga aaatgggaat
    34321 ttggttgcgt aaagctgttt tatttgcagc tgtgttaggg gctttggggg ctgtttcaaa
    34381 aactgttgaa gcatctgcca gcgaatttat tcgcggagca gatatttcga tcctgtcaga
    34441 tatggaaaaa tctggtgcaa aatattatga agacgatatt tcaaaagatg ccttagagat
    34501 tttaaaaaat aatggtgtca actatgttcg actgcgtttg tggcaggatc cttataatag
    34561 taatggagaa agttatggag cagggacaaa tgatctggaa acgacgattg accttgcaaa
    34621 acgagcaaaa aatctgggac ttaaggtgct gctggatttt cactacagtg atttttgggt
    34681 tgatcctggg aaacagaatc ttcctaaagc atggcaaggt ctgacctttg aagaaatgaa
    34741 tacggcattg tacgactata caaagaacgt tctgtcggaa atgaagcagc tggacgttta
    34801 tcctgatatg gttcagatcg gcaatgaact gaatagcgga atgctctggc cttatggcaa
    34861 gtcttgggga gaaggcgggg gagaatttga tcgattggct gcttttttga agtctggtat
    34921 ccaagctgtt cgagacactc aacctaaaac aactccagta atgctccact tagctgatgg
    34981 tggcgataca ggagcattta cttggtggtt tgatgaaatc acgagtcgag gtgtgtcatt
    35041 tgatttaatt ggagtgtcgt attatccgta ttggcacggt tcaatgaatg aacttcaaac
    35101 caatatgaat gcgatcagcc aacggtatgg taaagaagtg atcgtagttg aaacagctta
    35161 tgggcataca acagcgaatg ccgatactat gccaaatgct tttggtgaag ccgaagcagc
    35221 tgcgggggga tatgaaccaa caccgacagg acaagcagag tatttgctgg atcttgccga
    35281 tcggattcag gcagtaccga ataatcgcgg ggcgggcttc ttctattggg aacctctttg
    35341 gtacaacgga aatgtgagtt gggcaacaca agcaggtatg aattatctca gtgttcaatc
    35401 cactatggga aacgaatggg acaaccaagc gatgtttgat tttgctggaa acgccttgcc
    35461 aagcttgcgt gcgtttaaac aagcaggggc gcaaataaat ctagttaaaa atgcaagttt
    35521 cgaaagcgat ggaccaacga ccagtccttc caattggcaa acctggtttc aacaaggaac
    35581 cgctaagggt acgatcaaaa cggaagcggg cacttttttt ggggactaca aactaacttt
    35641 ttggcaggat caagcatacg aggcatcagc ttatcaaaat gttagtggca taagcaaagg
    35701 aacttacaca ttgagtgcct atgtaatgtc tgggggagag tttgatacta atcagttata
    35761 tatcaaaaat tatggtggag cggaactaaa tactgcggtt gttaaaacaa gtgaatggac
    35821 aaaaattaca atcaaagaca ttccaattac aacaggtgtt tgtgaaattg gggtttatag
    35881 caaggcttca ggaaatgctt ggacgaacgt tgattttgtt tcattggtga aacaatagac
    35941 tgcggatcgt ttcgattcaa gatcggaggt tcccttcagc cacatcgtta gcgtagaagg
    36001 caggaatcga acctgcacct cgcctttgcc aaaaataacc agaaacgatt tccagtcatt
    36061 tttaccaaaa cgagtaaagc accagacttc atctacactt tgtattatgc taaagagagt
    36121 tcaacgattt cgtaagaagc aatgaaaaaa ttgcaaaaag ttgtgcagat aacataaagt
    36181 ttgtttaaga aagggtaacc ttagaattcc gtcgtgaata tgatttgagc acttagatgg
    36241 atagaaaaaa gttctcggaa aacagttcgt ttttcgagaa cttcttttta tgataagaat
    36301 gaaacaaatt aacttatatt atagtctctt ttggtaggga accatcttat ttttaatgaa
    36361 aatcaagcat tttagctatt gaagaatgtt gatttatagg gattccccct ccatattatg
    36421 ttggctcttt attcccactc cactgttgct ggtggtttag aagtgatgtc atacacgata
    36481 cggttgacgt gggctacttc gtttacgata cgtacagaga ttttttgcaa gacttcccat
    36541 gggatacggg caaagtcagc ggtcattccg tcgattgaag ttacagcacg gataccgact
    36601 gtgtagtcgt aagtacgtcc gtcacccata acaccgacag agcggatccc tggaagaaca
    36661 gtgaaatatt gccagatatc acggtcaagt ccagcagctg cgatttcttc acgcaagata
    36721 gcatcagatt cgcgtacgat ttctagcttg tcttcagtga tttcgccaag aacacgaata
    36781 ccaagaccag gaccagggaa tggctgacgc caaacgattg aatcaggcat gcctaactga
    36841 gtccctaatt cacggacttc gtctttgaat aatgtgttta atggttcgat caattcaaat
    36901 tgcatatctt ctggcagtcc acctacatta tggtgagatt tgatcgtctg tgctgtttct
    36961 gtaccagatt cgataacgtc agtatacaac gttccttgtg ctaggaaaga gacgccttct
    37021 tcaccagcaa gttttgttgc ttcgtcatca aaaacatata cgaattcatt cccaatgatt
    37081 ttacgttttt gttctgggtc agatacacct ttcaacttgt ctaagaagcg atctcttgca
    37141 ttgactttga tgatgttcaa gccgaatttt ccgcctagac tttccattac ttgttccgct
    37201 tcgcctttgc gtagcagacc gtggtcaaca aagatagatg tcaattggtc accgatcgct
    37261 ttttgtaaaa gaaccccaac gacactagaa tcaacgccac ctgaaagacc aagcaagact
    37321 tttttgtcac caacttgttc acggattttt tctacttgca tatcgataaa gttatccatt
    37381 gtccagttac cttcacagtg acaaacatca aaggcaaagt ggcgcagcaa ttcgattccg
    37441 tattcggaat gacgtacttc tgcatggaat tggataccat aaaaattacg tgttgtgtct
    37501 tggatagaag caatcggaca atctttgctt gttccgactg tttcaaagcc ttcaggaact
    37561 ttagttacca agtcaccatg gctcatccat actgtttgtt gttttggtgt ttctttaaac
    37621 aagacagctt gatcgcttgt tacttctaac aaggctttcc catattcgcg gtttttagca
    37681 ggctctacgg aaccgccaag tttgtacgtg atcaattgca taccatagca aatccctaat
    37741 acaggaatac ctaaattgaa gatctcagga tcgatcccaa atgcattctc atcatataca
    37801 ctgtttggtc cgccagaaag aatgattccc tttggattca tcgcttttac ttcttctgca
    37861 gtaatgcggt ggctcaataa ttcggaaaag actccaagat cacgaatacg tcttgtgatc
    37921 aattggttat actgactacc gtagtctaat acgataatct tttcaacgtt cgtcatatcg
    37981 gcaacgtttg tcaaatttat tacccctatc cctttaaatt ttatctcaaa atagtcaaac
    38041 tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg atgatatgat tttcagtttt
    38101 atggagaatt atatcggctc ttcctcgtgt aggcaagata tattcctgta aatttttcaa
    38161 attcacattt ttccatacat cttttgccat tttaaaggct tcttcacgat ctccgatagc
    38221 aaaacgataa taataattct ctggattttg gaaagccgta tccagtaacg caccaaaacg
    38281 atccaaatac catttttcga tcagcacggg atcggcatcg acatagacag aaaaatcaaa
    38341 aaagtcgctg acgtagattt gctgattcgc aggtaattga agcgtattga tcccctcaac
    38401 gatcaagata tcaggctgct ggatcaattc gtacttgtct ggaatgatat catacacatc
    38461 atgtgagtaa acaggtactt ttatctcttc ctgaccagat tttacacggt tcaagaaatc
    38521 gatcagcatc tccatatcat aactttcagg aaatccttta cggtccatga ttccacgctc
    38581 ttttaatata cgatttggat aaagaaatcc gtcagtcgtg atcagctgaa cgtttcttcg
    38641 tttgaaggta cgagacaaaa tcgtctgcag cagtctggct gttgtacttt ttccaacggc
    38701 tacacttccg gcgatcccga taataaaagg cggtacttct acgtatttat gaagaaataa
    38761 acctttactc aagaacaaag attcaaattc tttcatataa agataaatca gatgagcaag
    38821 aggtacatag atatctcgaa catcctgcat ggaaatgcgg tcattcaaac ttttgatatt
    38881 atctagttca tcttgtgtca aaggtgcttg cccattacga taaaattctc gccattcttc
    38941 tcttgccaca cggtagtagt tcattggatc attcatatta gcctcctgcg ttctcccatc
    39001 aatggaacta ttttaacaca aaataaacgc aaaccccacc acttaagaga gattgccgat
    39061 ctttttgtat aatctttata atagaaagaa acggagtctt attcttgact tgcgcacgga
    39121 tacataaaag gatagactgt tgtcatgaaa gaaatgagaa ggatggaaga catgacaaat
    39181 cattactatt ctgaacgccc agatacggcg cacgattttg aacaatgggc ttttgagtta
    39241 aaaggcagaa cctttcaatt tgtaacagat tctggtgtat tttcacgtga aacagtggat
    39301 tatggttcac gtgtcctgat tgatgctttc gattgggaag agttacctga tgaaggaaag
    39361 atcttagatg tgggatgcgg gtatggaccg attgggttag ctttggcatt tgcgacacag
    39421 cggtttgttg aaatggtcga cattaatgaa cgagcggtaa gtctagcgca aggaaacgca
    39481 aagcgtaacc aaatcgaaca tgttgatatc catcaatcga atatctatga agcagttcat
    39541 gaagaaacct atgcagctat cgtaagcaat ccgccgatcc gtgctggaaa aaaagttgta
    39601 catgaaattt tgacaggcgc atatcctcgg ttgaagaagg gcggtacact gacaattgtg
    39661 atccagaaga aacaaggagc gccgagtgcg caaaagaaaa tggaagaaac atttggtaat
    39721 gcagaaatcg tcacaaaaga taaaggatat tatatcatca aaagtgtgaa agaataacta
    39781 gctgaaaaat agtggagcga cgaaagagtg gaactgggcg tgcatgtacg ggtacgtatc
    39841 agttcttttt ttgttgagaa gtttttaaaa acaaagaact attttttctt caaaaagaaa
    39901 tagtaggaac agagtcgttg ttttttacga taaaataaat aagatacaga acttgtatag
    39961 aaggctgcca agtcggtaga taaaggaggt ttttatcaaa aaacatactt tccttttttc
    40021 tctattcttt tcttgggggt tttggtacac tgatagggaa tttagataga agggagccaa
    40081 taggatgaag ctgaccatca gagatatcgc agaaatggca ggcgtatcag taacaactgt
    40141 ctcacagatt ttgaataaca aaggaagcag atttagtgat aagacaagaa aaaaagtgtt
    40201 gtcgatcgtg aatgaacatc attataagcc cgattatttc gcttcaaatg tgatcaatcg
    40261 acattcaaag accatcggaa tgatcgtccc tgatgtcact gatttcttct tctccaaact
    40321 gatcgaaggt gtagagagct atttgaatcc tttgggctat gtgatcatgt tatgcaattc
    40381 tcgtcatagc caagaaaatg agatcaagta tttacaagag ctttcccacc gctcagtgga
    40441 cggaatcttg ctggccacac caaatatttt acctgagaaa tatgcattag acagcggttt
    40501 ttatcaaaaa atgccaatca ttttaatcga tcgcggattg aatcgtcggg ataacggacg
    40561 gcttatcgta aaggaatatg agggcgccta tcaagctgtt tcgctgttta ttgaaaatgg
    40621 ccatacgaag atcggcatgc tgaaggaaac cactggatac tatcaattag aagaacgttt
    40681 caacggatat cgtcatgcgc tgaaagatca tggcctgcct tttaatggga aatttgttga
    40741 acatggagag ttgactgtcc aaggtggata tactgcatca aaaaaattac tcaaacataa
    40801 agatatcacg gctatttttt gcgggaatga tgccatggcg atcggatgct atcaggcgat
    40861 cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc ataggattcg atggattaaa
    40921 gctgtcagaa tacatgattc cgcaattgac gacagtgcag cagccaagtt ttgatattgg
    40981 attttatgct gcacgtttcc tgatcgatac gatcgaattt cctcaaagaa aagttccgaa
    41041 caaagtgttc gaaacaaaat taattattcg tgagagcgta aaagtattga caaagggcta
    41101 actcggcggt atattgctta tgtaagcatt tacaaaggct agttagtgct ttttttattt
    41161 ttagtaaacc agtttactaa agtggtttac ctttcctaaa aataatgtat aataccgaat
    41221 gaagacaagg aggatcaggc aatgcgaatg gtggatctga ttgaaaaaaa gagggatgga
    41281 aacgagctgt cgaaagagga aatcgagtac atcgtcacga actatacgaa tggaaagatt
    41341 ccagattatc aagtcagtgc attattgatg gcaatttttt atcaagacat gacaaatgaa
    41401 gaaatcacga acttaacatt ggcgattgct aattctggtg atgtcatcga tttgagttct
    41461 cttgaaggaa taaaggtaga taagcattcg acaggtggcg ttggtgacac gaccacattg
    41521 attttagccc ccctagtagc tagtgtcgga gtgacagtgg ctaaaatgtc tggacgcggt
    41581 ctaggttaca caggtggtac actagacaag ctagaagcaa ttcccggatt tcagatcgag
    41641 ctttcggatg aagaatttgt acgcattgtt aatgaaagta aagtagcggt aattggtcaa
    41701 tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct tgcgtgacgt cacagcgacc
    41761 gttgattctc ttccgctgat tgccagttcg attatgagta aaaaaatcgc ggcaggagca
    41821 gatgcaatcg ttttggatgt gaccactgga gacggtgctt tcatgaagaa catcgaagat
    41881 gcaagacgtc tggcaaagac catgacgagc attgggaaac tggctaaccg agaaacagtg
    41941 gcggttatct cagatatgtc agaacctctt ggcgaagcta tcggaaatag tctagaagtt
    42001 gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag atctagtaga aatgtgctac
    42061 gccttaggaa gccaaatggt tgttcttgct ggcaaagcta agacaaccga tgaagcaaga
    42121 acgttgcttc aagaagctct ggaatcaggt aaagcgttag caaaattcaa agaaatgatc
    42181 caaaatcaag ggggagaccc agcgatcgtg gaacatccag aacgaatttt gactgcacgc
    42241 tacacaatgg agcttcctgc taaacaaagc ggagttgtgt caaaaatcgt ggcaaatgaa
    42301 ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa ccaaagaaga agatattgat
    42361 cacgctgttg gattgaaact gcataaaaaa attggtgaca ctgtgacaaa aggtgagtca
    42421 ctattgacga tctatagtaa cgacgaagaa atcacttctg tcattgaatt attatataaa
    42481 aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga ttcatgacat catcacagaa
    42541 taaaggaaaa aagcggactt taccgtttta aagaaaaggg gaaaagaaat ggaactaaac
    42601 cgaatgatcg accatactat tttgaaagca gacgcatcta aagaagctgt gatgcaaatt
    42661 atcgaagaag caaaaaaata ccatttttat tctgtctgca tcaatccagc atgggttgcg
    42721 ctagcgaaag aacaattaca aggtgagcca gtagccgtat gtacagtaat cgggtttcct
    42781 ctaggagcaa acacacccga gacaaaagca tttgaaacaa cagatgccat caataacggt
    42841 gcagatgaag tcgatatggt catcaatatc ggtgctttga aatcaaagga tgatcaacaa
    42901 gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag atcgtgcgct tgtcaaagtt
    42961 attattgaaa catcgttatt agatagagaa gaaatcatcc gagcttgcga aatagcaaaa
    43021 gctgcaggcg ctgattttgt caaaacatca actggtttct caacaggcgg agcaaaggta
    43081 gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa tgggtgtcaa agcttccggc
    43141 ggtatccata acgaagaaga agcaatggca atgatcgaag ctggtgctac aagaatcgga
    43201 acaagcgcag gagtagcaat cgtatctggt tcaactggtg aaggctatta attttttcaa
    43261 aggagaagct catggcagaa ctcaaacaag aatggatcaa cacagctatt gaagcgttag
    43321 ataaagccta tgttccttat tcccattttc cggtaggcgc ttgtctcgta acggaatcag
    43381 gcaagattta tcaaggcatc aacattgaga atgcatcatt tggattaacg aattgtgccg
    43441 agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa aacatttacg catctagtcg
    43501 tagcggggca cacccccgat cctatctctc cttgtggtgc atgtcgacaa gtaatggcag
    43561 aattctgttc ccctgatatg ccggttacgc tagtaggaga caatggtgtg acgaaggcaa
    43621 cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa agatctataa ctcatgttca
    43681 ttcagtcagc tgactgttga aaaataaaac aattatttta ggaggcttta cagaaatgaa
    43741 aaaagcaaaa ttatttggtt taggtgctgt cgcactagca gcagggttat ttttaggtgc
    43801 atgcggcaac aacggctcta ctgattcatc cggtggcaaa agctcatcag acacaacaac
    43861 tgcagcattg atcacagaca caggtggtgt agacgaccgt tcattcaacc agtcagcttg
    43921 ggaaggacta gaaaaatggg ggaaagatca aggactttca cgaggaaatg atggcttcca
    43981 atatttccaa tcttcaaatg aatcagatta catcccaaat attgaccaag cattgaatgc
    44041 tggattcaaa acgatttttg gtattggtta taaactaaaa ccagcgatcc aagaacaagc
    44101 aacaaacaat acaggtacga actttgttat tatcgatgat gtcatcgatg gattagataa
    44161 cgttgtatct gcaactttca aagacaacga agcttcttat cttgcaggtg ttgcagcagc
    44221 ttatacgact gaaacaaacg tagtcggctt tatcggcggt gtaaaaggtg aagtaatcga
    44281 tcgttttgat gctggattca aagccggtgt tgatgcaggt gcgaaagaat taggaaaaga
    44341 aatcaaagta ttgaatcaat atgctggtga cttctctgct ccagataaag gacgttcaat
    44401 tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc catgcttcag gcggtacagg
    44461 taacggtgta ttccaagaag caaaatcatt gaacgaatca ggcgacaaaa aagtttgggt
    44521 tatcggtgtt gaccgcgacc aatcagacga aggcgaatac acattaaacg gcgaaaagaa
    44581 aaacttcaca ttgacatcta cactgaaagc agttggaaca gtagtagaag atttggcaca
    44641 aaaatcagcg gacggtaaat tccctggcgg cgaacacact gtttatggat tgaaagaaga
    44701 cggtgttggt ctaacagaag gacaactttc tgacgaagcg aaaaaagcag ttgatgaagc
    44761 aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa actcctgaag aaaactaaaa
    44821 ccatttctat agccttgtta gacacatgtt tgacaaggct atggtttgat tagaacaaga
    44881 attgttcatt tatccaagct gccttgtgac agtttggata agggaacaac tccctgaagt
    44941 aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat cttagtcaat cgttgcccta
    45001 gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga aaatattgtc atagaaatgc
    45061 agaatattac caaagaattt ggaacattca aggcaaatga caacatcaat ttacaagtaa
    45121 aggcaggaga aattcatgcg ttgcttggag aaaacggtgc tggcaaatct acattgatga
    45181 acgtgctttc cggattatta gagccgacat cagggaaaat tttgatgcgt gggaaagaag
    45241 taaagatcac aagtccgacc aaagccaacc aattagggat tgggatggtc catcagcact
    45301 ttatgcttgt tgatgccttt actgtaacag aaaacatcgt gttgggaagc gagcctagtc
    45361 gcgcagggat gcttgaccat aaaaaagcac gaaaagagat ccaaaaagtt tctgaacagt
    45421 atggattgtc agtcaaccca gatgcttacg ttcgtgatat ttcagttgga atggaacaac
    45481 gggtagaaat tttaaaaaca ctttaccgag gagcagatgt actgattttt gatgagccga
    45541 cagctgtatt gacccctcaa gaaattgatg aattaatcgt gatcatgaag gaattagtca
    45601 aagaaggcaa gtcgatcatt ttgattacgc ataagttaga tgaaatcaaa gcagtagctg
    45661 accgctgtac agttatccgc cgtggaaaag gaatcggtac agtcaacgtt aaagacgtaa
    45721 cctcacaaca attagccgat atgatggttg gaagaacggt ttcattcaaa acgatgaaaa
    45781 aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa tctagtggtg aaagaaaatc
    45841 gtggattaga agccgtgaaa aacttgaact tagaggttcg tgctggcgaa gtacttggta
    45901 tcgctggaat cgatggaaac gggcagtcgg agttgatcca agctttgact ggtttgcgaa
    45961 aggcagaaag cggacatatc aagctaaaag gggaagacat cactaataaa aaaccccgaa
    46021 aaatcactga acatggtgta ggacacgtgc cagaagaccg tcataaatac gggttagtcc
    46081 tagatatgac attatctgaa aatatcgcac tacaaaccta tcatcaaaaa ccttacagta
    46141 aaaacggcat gctgaattat tcagtaatga atgaacatgc cagagaattg atcgaagaat
    46201 atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc tttatcaggc ggaaatcagc
    46261 aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga tctgttgatt gttgcaaatc
    46321 caactcgtgg actggatgta ggtgcgatcg aatttattca taaacgtctg atcgaacaaa
    46381 gggacaaata caaagcagtg ttactgatca gtttcgaatt agaagaaatt ttaaatgttt
    46441 cggatcgtat tgctgttatc catgaaggag aaattgtcgg tatcgttgat ccgaaagaaa
    46501 catctgaaaa tgaattaggt ctattgatgg cgggctatac cttagaagaa gcacgaaaag
    46561 agttagaaaa gggggctggc gcacatgaat gatcgcagcg aaaaattccg taatttatta
    46621 gtgcctgtac tttcagttgt tttaggattg atcttaggcg cgatattgat ggctgctttt
    46681 ggttttcagc ccatccaagg ttattcttct atgctagatg cagcccttgg ttcgcaaaga
    46741 gggataggtg aaacgttaag gcaagcaacg ccgttgattt ttacagcatt aggtttttct
    46801 gtagctaact cagctgggtt tttcaatatt gggctttctg gacaagcact ttgcgggtgg
    46861 atcgtcagtg tctgggtggc acttgcgttt ccagatctgc caaaagttgt tttgcttccg
    46921 ttatgcgtga tcttaggtgc cttagctggt gcgatggcag cggctgttcc aggtttactt
    46981 cgagcatttt ttggaacaag tgaagtgatc gtaacaatca tgatgaacta tatcttatta
    47041 tacgtaagca catttgcttt acatgaagtg atgcctgaaa gctatcgctc aagtttggac
    47101 tcttccaata tgatcagtca aaatgcctca ctgcggttac catggttaac acaagctttt
    47161 ggcggctctc gcgtaaactc aggattattt ttagcaatca tcgcgcttat tttcgtttgg
    47221 ctgctgatga agaaaacgac tttaggattt gaaatccgtt ctgttggatt gaatcctttt
    47281 gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg ttttgtcgat ggtcatttct
    47341 ggcgctttgg cagggcttgg cggtgtggtt gaaggattag gaacgtatca aaacttcttt
    47401 gttcaaacga cttcgctatc tattggattt gacggaatgg ctgtttcctt gcttggttct
    47461 ggatcagcaa tcggtattct tttatcagcg atcttgttca gtattttgaa gatcggtgga
    47521 cttggcatgc agacaggagc aggtgttccg tttgagatcg tcaatgtatc gattgctttg
    47581 attatcttct ttgtcgggat caacttcctg attcgtttcg tcatggctaa agtcttccaa
    47641 gggaaaaaac aagaagagat cgttgcgaca atagaatcga aaccaacgaa tcaaaaccat
    47701 gaagggggcg aactataatg tcgactatcg aattaattgc cttgatcctt acttctacac
    47761 tggtttattc aacaccgcta attctaacgt cactaggcgg aactttttct gaacgaagcg
    47821 ggatcgtaaa cgtagggcta gagggaatca tggtcatggg tgcttttagt tccatcgttt
    47881 tcaacttgac gatgagtcca tcacttggtt ctatgacacc atggatcgct attttagtgg
    47941 gtggcgtcgt aggtatccta ttttctctga ttcacgcagt agcaacagtc agtttgcgtg
    48001 ctgaccatat tatcagcggg acagtaatta atctgatggc acctgcttta ggtgttttct
    48061 tgatcaaagc atggtatggt aaaggtcaaa ctgataacat cacgcagaat ctggggtatt
    48121 tttctttccc aggattagca gatattccgg tcattggacc aattttcttt aaaaatacat
    48181 tcttacctgc ttattgtgct attttgattg ctgtcctagc ttgggtgatc atcttcaaga
    48241 cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc tcaagctgcc gacacattag
    48301 gtatcaatgt atatgctatg cgttattcag gcgtactgat ttctggcttg ttaggcggta
    48361 tgggcggtgc tgtttttgct cagtcgattt ccggtaactt ttcagctggt acgatcgttg
    48421 gacaagggtt catctccatg gctgctatga tctttggtaa gtggaatcct ttaggggcaa
    48481 tgggtgcctc attgttcttt ggttttgctc aaagcttgag tatcattggc gcacaattac
    48541 cagttatttc ttcgattccg ccagtattgc tgcaaattgc tccatatttg ctgacaatca
    48601 ttgtgctggt tgttttctta gggaaagctt ctggtccaaa agcaaacggg aagaactata
    48661 tcaaatcaaa ataatctttt agcgaataaa aagggtgggt tacgggaagt gtttttcgtc
    48721 tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa gaaggaataa tcatgtttaa
    48781 acgcgtacat ctgatcgtca tggattcagt aggaattgga gaagcaccag atgccgataa
    48841 gttcggtgat gttggttctg atacattagg acacattgcg aaagaagcag gattgaacgt
    48901 tccacattta gagcagcttg gtttaggaac gatccgtcct cttgaaggtg tggaatcagt
    48961 gaaagatcat caaggctatg cgacgaaatt agaagaagtt tctgtaggga aagatacgat
    49021 gacaggccac tgggaaatca tgggattgaa tatcaaaact ccatttcgtg tgtttcctaa
    49081 tggattccct gatgatctga tcaaacaaat cgaagaattt tctggccgta aagtcgtatg
    49141 taacaaacct tatagcggaa cagctgttat tgacgattac ggtgaacacc aaatgaaaac
    49201 aggcgattta attgtatata cttctgccga tcctgtcctt cagatcgcgg cacatgaaga
    49261 aatcattcct ttagaagaat tatatcggat ttgccaattc gttcgtgata taacaaaaga
    49321 tgatccgtat atgattggcc gaatcattgc acgtccgtac gtgggtgaac caggagactt
    49381 cacacgaaca agcaatcgcc atgattatgc gttgaatcca ttcggacaca cagtccttga
    49441 ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga aagatcaacg atattttcaa
    49501 cggacaagga atcactgaat ctgtccgcac gaaaagcaat atggatggcg ttgaccagct
    49561 gctgaaagtt atgaaaaacg atttttcagg aatcagcttt accaatttag tagattttga
    49621 tgcattatac ggtcatcgtc gggatgtagt cggctatgcg catgcgattg aagaatttga
    49681 cttacgaatt ccagaattgc ttgaagcgat ggaagaagac gacttgctaa tgattacagc
    49741 cgatcacgga aacgatccga cgtttactgg taccgatcat acgcgagaat atgtaccgtt
    49801 attggtttac agtaaaaaaa tgaaagaaca aggggcattg cctcaaggat attattcaga
    49861 tatttcagca acgattgctg agaactttag tgtttcttcg actgaaaatg gcgaaagctt
    49921 cttgaaatta ttaaaataat ctttttaaat aggagaaagc aaatgaccaa gttaagcgag
    49981 atgttacaag aaacgaccca gtttatcctt gataaaggag taaaagaaat tgattttggt
    50041 ttgattctag gttcaggatt aggtgaatta gcagatgaaa tcgaagatgc tgtaatgatt
    50101 tcttatgatg agatcccgtt ctttcctgtt tcgacagtag ttgggcatgc aggaaagcta
    50161 gtttatgggt ctctggcagg caagaaagtg ttagccatgc aagggagatt ccactactat
    50221 gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg tgatggccgg actaggggcc
    50281 cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg aatcatttgt accaggagat
    50341 ttgatgctga ttaccgatca tatcaatttt actggtgaaa atcctttgat cggtccgaat
    50401 gaagatgcaa tgggccctag atttccagat atgagtcaag cttatacgcc tgactatcaa
    50461 gcaacagcaa aagcagcagc agaaaaacta ggtttggatc ttaaagaagg cgtatatatg
    50521 ggctattctg gaccaactta tgaaacgcct gcggagatcc gaatgtctcg gacaatagga
    50581 gccgatgcag tagggatgtc gactgttcca gaagtcatcg tagcagctca cagcggatta
    50641 aaagtgttag gaatctcttg tattaccaac ttagcagcag gtatgcaagc gaatctgagc
    50701 catgaagaag ttgtagaaac aactcagaga gtcaaacaat cttttaaagc attggttaaa
    50761 gaaacattga cattattgta ataggaggaa caataaaatg agtgtgcata ttgaagcgaa
    50821 agaaggcgag atcgccgata agattttact tccgggagat cctttgcgag ctaaatatat
    50881 tgcggagact tttttggaag atccagtttg ttacaatcag gtacgcggta tgttaggctt
    50941 taccggcaaa tataaaggag aacgtatttc tgttcaagga acaggaatgg gaatgccatc
    51001 agccaccatc tatgcccatg aattgatcca gtcgtatggt gtaaagaaat tgattcgagt
    51061 aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt gatttagtac tagcacaagg
    51121 tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa gcctttcatt ttcctccaat
    51181 cagtgatttc aatctattac tgaaagcata tgagatagct aaagaaaagg gctatacggt
    51241 tcatgtagga aatgttcttt cggaagattc tttctacaaa gatgatttga cagagacgtt
    51301 ccaattagct gaacttggcg ttttaggagt ggaaatggaa gcagcggctc tttattactt
    51361 aggcgcgaaa taccatgtgc agacattaag tttgatgacc gttagcgacc atttgatcac
    51421 cggtgaagaa acaacagctg cagaacgtca atctacgttc aatgaaatga tcgaagtagg
    51481 attggaaaca gcgatagcag aataagaagg aaatgaaaaa gctgccctgc attaagtaat
    51541 aagaacatcc aagcaaaaat agctcctcat gtttttgact tggctggact tattattgca
    51601 aatgtaagct tttgaacacc gtttaccaag aggttgtgaa tctgccgttt aactctgagt
    51661 cacaaggaac aatttcaaaa aacagctttt catgtttgat gaatattgtg acttgtgacc
    51721 gagaagttgg caggagaaaa ccgtttatac gaaaaaaggg actgtgacaa atcgtgtcac
    51781 agtccctttt ttatagtgtt ttttataggt agtatttttt agcaactgtt aagtcatcat
    51841 ttaattcata aacaagtggt tgacctgttg gaatttcaag atccatgatg tcttcgtcag
    51901 agataccttc gatatgttta gctagggcgc gtaatgagtt accatgtgca gctactaaaa
    51961 cagttttgtt gtctagtaat gctggagcaa tttcatcttg ccagaatggc aaagcgcgtt
    52021 ctaatgtcac tttcaagttt tctccaccag gaatatcgcg tttgtctaac attgcgtaac
    52081 gacggtcatt tgctgcagaa ccttcgtcag tttcttccat taatggagga agagtatcgt
    52141 aagaacgacg ccagatgtgc acttgttcgt cgccgtattt ttcagctgtt tcttttttgt
    52201 tcaaaccttg taatttacca tagtgacgtt cgtttaagcg ccatgatttg atttgtggta
    52261 cccaaagttg atctgaatat tctaagatca agttacatgt tttgattgca cgagttaaaa
    52321 cagaagtgta agcaacatca aattcgattc cagcttcttt gattttgcgt ccgccttctt
    52381 ttgcttcttc gattccttct ggtgctagat ttacatcagc ccaaccagta aattggttca
    52441 atgcattcca ttcactaaga ccgtgacgag aaaaaactaa ttttggcatg tttagctctc
    52501 cttttaagat aggtttatat ttctgaaatt ttcagttctg ttttattgta caattttttg
    52561 gaagagattt ccatcgaaaa attagataag attctatgag aaaacaagta gagaatataa
    52621 aaaaacgaac agtgcccacg caactgttcg tttttaggta ttagatgcat aattgctcga
    52681 tttctttttc aggtaaattg atatatttat aacatgttcc aactgaaaca ccgcattttg
    52741 aagaaataaa ttggatcgtt tctttttttt cataatagag acgtcggatt tttttgatcg
    52801 tttttgcatc gatttttggt cttccgccga tttttccttt tttacgagct tcatctaatc
    52861 caactaatgt acgttctttg atcaatgcgc actccatgtt tgctaggttt tccattaatt
    52921 ggaaatagat ttttcccatt tcgcgcgtat cgattccttc atcaagacta atcaagtgca
    52981 gcccgcgatc tttgaataat tgcgtgagtt ctgttaattg ccgcgtagat ttgcctaatc
    53041 gatgaagttg gcaaatgact agtgcatcgc cttgttgcat gtcagcgatc actgactcca
    53101 aatgaacagc agtagaatca ttttgttcca cttcatatgt ctcatggaaa atttcttgac
    53161 aaccaaagtc agataattga tcaacctgag ttgttaaatc aacgtcattt atggttgtgc
    53221 gtgcataacc aataattttc atggtgattc ctttctagca gtgattttct aactggatat
    53281 aacagtaaaa atgtagcata aagtacgtca aaaagatagt tttttcgcaa attttccgct
    53341 gtcttttttt attggatgat caagcagttt ttctttattt acgacggatt gcgtcaagaa
    53401 tctcaacgcc tttttctgag ccgacaacta ctttagaaac catgtttagg aaaagcccat
    53461 gttctactac cccaacaagt tcatctaaat aggtagctaa gctttctggc tgttcgatga
    53521 cttctagatg cagatcaatg atgtaatgac caccatctgt ggttaagact tctccgtttt
    53581 cgtctgtacg caagacgggt tggaatcttt tttcttcaaa aatatgaagt agctgttggg
    53641 agccataagg gatcacttca actgggagtg ggaacttccc aagtttatcg actaatttag
    53701 aagaatcaac gatccaaatc gtttcttttg agtacgtagc gactattttc tcgaaaagca
    53761 aagcagcgcc gccgcctttg atcccttgaa aatcagcact gatttcatca gcaccgtcta
    53821 ttgtcaaatc gactacgggg acttcatcaa tactctttaa cggtattcct aatttttccg
    53881 cttgttcttt tgtcgcattt gaagtagtaa ctcctgtgat acgtaaacct tcttcacgca
    53941 tacggcgacc aatctcttct accatataat aggcggttga accggtcccg agacctacga
    54001 tcatcccgtc ctttacgaat ttcgcggctt caatgccagc taattgtttt aaattcatct
    54061 ttgcgtccac ctttttttag tttgagcatc gtgctttcct cactatttta gcgtgattta
    54121 cggtaaaaag ataccattct tttttattcc aataaagctt tttcaaaaaa aattgcatct
    54181 cttgttgaca tgttttttca ttcgtgatat tctaacaaag gtgctttatc tacaggtttc
    54241 tgtgaagaaa cgtgctgact gtttatcaaa gcatatttga taaggatgca gacattgcat
    54301 ttatttttta tcacaaaaaa agaaccacct ggatgtgtgg aactagacgc aaatcgtggg
    54361 aaggaggaaa caaggaatgc ctacaattaa ccaattagta cgtaaacctc gtaaatcaaa
    54421 ggtggaaaaa tctaattcac ctgctttgaa caaaggatat aacagcttta agaaaactca
    54481 aactaacgta aactcacctc aaaaacgtgg ggtagcaact cgtgtgggta caatgacacc
    54541 taaaaaaccg aactcagctt tacgtaaata tgcacgtgtt cgtttgtcaa acttaattga
    54601 agttactgct tatatcccag ggattggtca taacttacaa gaacacagtg ttgtattatt
    54661 acgcggtggt cgtgtaaaag acttaccagg ggtacgttac catatcgttc gcggtgcttt
    54721 agatactgct ggtgttaacg accgtaaaca aagccgttct aaatacggaa ctaaaaaacc
    54781 taaagcttaa ttttaattat taaacaaatg aaacttgctt ctactatata gaagtaatta
    54841 ctatgaacaa tatttcggaa ggaggagtta cggatgccac gtaaaggtcc tgttgcaaaa
    54901 cgtgatgttt taccagatcc tatttataac tcaaaattag taactcgctt gatcaaccgt
    54961 gtaatggttg acggaaaacg cgggattgca gctaacatta tctataatgc ctttgacttg
    55021 atcaaagaat ctacaggtaa cgatccatta gaagtgtttg aacaagcaat gaaaaacgtt
    55081 caacctgttc ttgaagttaa agcacgtcgt gttgggggtt caaactacca agtaccagtt
    55141 gaagttcgcc ctgaacgccg cgttactcta ggtcttcgtt gggtagtaaa ctacgctcgc
    55201 ttgcgcggtg aacacacaat ggaagaacgc ttagcaaaag aaatcatgga tgctgcaaac
    55261 aacacaggtg cttctgttaa aaaacgtgaa gacactcata aaatggctga tgccaaccgt
    55321 gcgtttgctc actatcgctg gtaagattct ctctgtttgg tcggctttta taagtcgacc
    55381 tcagatagtg tccttatttt tagcaactag atagtaaaga gaggagaaac aaggagaatg
    55441 accagagaat tttctttaca aaacactcgt aatatcggta tcatggccca catcgatgct
    55501 ggtaaaacga ctgctactga acgtatcttg tactatactg gtaaaatcca taaaattggt
    55561 gaaactcatg aaggagcttc acaaatggac tggatggaac aagaacaaga acgtggtatt
    55621 acgatcactt ctgctgctac tacagcacaa tggaagggtc accgaatcaa catcatcgac
    55681 acaccaggac acgtagactt cacagttgaa gttgaacgtt cattgcgtgt acttgatggt
    55741 gctgtaactg ttcttgatgc acaatcaggt gttgaacctc aaactgaaac agtttggcgt
    55801 caagcaacaa cttatggtgt acctcgtatc gtatttgcaa acaaaatgga caagatcggt
    55861 gctgacttct tatattcagt atcaacttta catgaccgtt tgcaagcaaa cgctcaccca
    55921 atccaattac caattggtgc ggaagacgac ttcacaggaa ttatcgacct tgtaacaatg
    55981 aaggctgaaa tgtatacaaa cgatcttgga acagagatcg aagaaacaga aattcctgaa
    56041 gaataccgcg aattagctga agaatggcgc gaaaaattgg ttgaagctgt agctgaaact
    56101 gatgaagaac ttactttgaa atacttagaa ggtgaagaaa tcactgaagc tgagttaaaa
    56161 gaaggtatcc gccgcgcaac agttaacgta gaattctacc cagtactatg tggttcagca
    56221 tttaagaaca aaggggttca attactattg gacgctgttc ttgattactt gccatcaccg
    56281 ttagatatcc cagctatcaa agggattgac cctaaaactg atgaagaagt agaacgtcct
    56341 gcagacgaca gcgctccttt ctcagcttta gcatttaaag ttatgactga cccattcgta
    56401 ggtcgtttaa ctttcttccg tgtgtactct ggtgttttac aatcaggttc atacgtacaa
    56461 aacgcaacaa aaggcaaacg tgaacgtgtt ggtcgtatcc tacagatgca tgccaactct
    56521 cgttcagaaa tctctgaagt ttacgctgga gatatcgctg ctgctgttgg tttgaaagac
    56581 acaactactg gggacacact atgtgatgaa aagagtctag ttattcttga atccatggaa
    56641 ttcccagaac cagttatcca agtcgctatt gaacctaaat caaaagctga ccaagataaa
    56701 atgggtgtgg ctttacaaaa actatctgaa gaagatccta ctttccgtgc tgaaacaaac
    56761 gtggaaacag gcgaaacaat catcgctggt atgggtgaac ttcacttaga tatcatcgtt
    56821 gaccgtatgc gtcgtgaatt caaagttgaa gctaacgtag gtgcgcctca agtatcttac
    56881 cgtgaaactt tccgtgctgg cactcaagcc gaaggtaagt ttgtacgtca gtctggtggt
    56941 aaaggtcaat acggtcacgt atggattgaa tttacaccaa atgaagaagg tgctggtttc
    57001 gaatttgaaa atgcgatcgt cggtggggtt gttcctcgtg aatacattcc agctgttgaa
    57061 actggtttga aagatgctat ggaaaatggt gtcttagctg gctatccatt agtagatatc
    57121 aaagctaaac tttacgatgg ttcataccat gatgtcgatt caaatgaaac tgccttccgt
    57181 gttgctgctt ctatggcatt acgtgcagca gctaagaaag ctaatccagt aatccttgaa
    57241 ccaatcatgg ctgtagaagt tgttatccct gaagattatt taggtgatgt tatgggtcac
    57301 gtaactgctc gtcgtggacg tgttgaaggt atggaagctc gcgctggcgg ccaacaagtt
    57361 gttcgtgcga tggttccact tgctgaaatg tttggttatg ctacaactct tcgttctgct
    57421 actcaaggtc gtggtacatt tacaatgaca tttgaccact acgaagatgt accaaaatca
    57481 gtacaagaag aaatcattaa gaaaaacggc ggaaaagctg aataatttct tgaaaccgga
    57541 tagtcctgca aagagattat ccaaagagac tatattttta atatagtttc gtgtaaaata
    57601 attatcgatg atatgggcat gtggtgtacc ttgtgtacat catataccta tcagacaaat
    57661 aaaaaatata ttgtattttt aggaggacat tttaaaatgg caaaagaaaa atttgaccgt
    57721 tctaaaccac acgttaacat tggtactatc ggacacgttg accatggtaa aactacatta
    57781 acagctgcaa tcacaactgt actatctaag aaaaacggcg gccaagctat ggcttacgat
    57841 caaatcgatg gtgctccaga agaacgcgaa cgcggaatca caatctcaac agctcacgtt
    57901 gagtatgaaa cagatacacg ccactacgct cacgttgact gcccaggaca cgcggattac
    57961 gttaaaaaca tgatcactgg tgctgctcaa atggacggag ctatcttggt agtttctgct
    58021 gctgacggcc caatgcctca aactcgtgaa cacatcctat tgtctcgtca agttggtgtt
    58081 ccttacatcg ttgtattctt gaacaaagta gacatggttg atgacgaaga attactagaa
    58141 ttagttgaaa tggaagttcg tgacctatta acagaatacg aattccctgg tgacgatgtt
    58201 cctgtagttg ctggatcagc tttgaaagct ctagaaggcg acgcttcata cgaagaaaaa
    58261 attcttgaat taatggctgc agttgacgaa tacatcccaa ctccagaacg tgacaacgac
    58321 aaaccattca tgatgccagt tgaagacgtg ttctcaatta ctggacgtgg tactgttgct
    58381 acaggtcgtg ttgaacgtgg acaagttcgc gttggtgacg aagttgaagt tgttggtatt
    58441 gctgaagaaa cttcaaaaac aacagttact ggtgttgaaa tgttccgtaa attgttagac
    58501 tacgctgaag ctggagacaa cattggtgct ttactacgtg gtgttgcacg tgaagacatc
    58561 caacgtggac aagttttagc taaaccaggt acaatcacac ctcatacaaa attctctgca
    58621 gaagtatacg tgttgacaaa agaagaaggt ggacgtcata ctccattctt cactaactac
    58681 cgtccacaat tctacttccg tacaactgac gtaacaggtg ttgttgaatt accagaagga
    58741 actgaaatgg ttatgcctgg cgacaacgta acaatggaag ttgaattaat ccacccaatc
    58801 gctatcgaaa acggtactaa attctcaatc cgtgaaggcg gacgtacagt tggtgccggt
    58861 gtcgttacag aaatcaaagc ttaattcact taagttttaa agtaaagcaa catttattcg
    58921 gacgtaatcg ctcggaacga aggtcttagg atctttgttc cgagcttttt t
//
LOCUS       DACCCS010000010        57299 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.010, whole genome shotgun sequence.
ACCESSION   DACCCS010000010 DACCCS010000000
VERSION     DACCCS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.010"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(26..328)
                     /locus_tag="IVK82_000656"
     CDS             complement(26..328)
                     /locus_tag="IVK82_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6391739.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="IVK82_000657"
     CDS             complement(353..2446)
                     /locus_tag="IVK82_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6391740.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="IVK82_000658"
     CDS             complement(2935..3699)
                     /locus_tag="IVK82_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP6391741.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IVK82_000659"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IVK82_000659"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP6391742.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="IVK82_000660"
     CDS             complement(4704..5780)
                     /locus_tag="IVK82_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP6391743.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="IVK82_000661"
     CDS             complement(5804..6397)
                     /locus_tag="IVK82_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP6391744.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IVK82_000662"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IVK82_000662"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP6391745.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVAIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="IVK82_000663"
     CDS             complement(8067..8366)
                     /locus_tag="IVK82_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP6391746.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IVK82_000664"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IVK82_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP6391747.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="IVK82_000665"
     CDS             complement(10121..11032)
                     /locus_tag="IVK82_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP6391748.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="IVK82_000666"
     CDS             11152..12009
                     /locus_tag="IVK82_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6391749.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="IVK82_000667"
     CDS             complement(12196..12558)
                     /locus_tag="IVK82_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391750.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="IVK82_000668"
     CDS             12904..14343
                     /locus_tag="IVK82_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6391751.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="IVK82_000669"
     CDS             complement(14350..16902)
                     /locus_tag="IVK82_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP6391752.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="IVK82_000670"
     CDS             complement(17016..18347)
                     /locus_tag="IVK82_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6391753.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="IVK82_000671"
     CDS             complement(18313..19791)
                     /locus_tag="IVK82_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6391754.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IVK82_000672"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IVK82_000672"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP6391755.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="IVK82_000673"
     CDS             22401..23828
                     /locus_tag="IVK82_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP6391756.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="IVK82_000674"
     CDS             complement(23920..24285)
                     /locus_tag="IVK82_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP6391757.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="IVK82_000675"
     CDS             complement(24282..24647)
                     /locus_tag="IVK82_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP6391758.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="IVK82_000676"
     CDS             complement(24839..25087)
                     /locus_tag="IVK82_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391759.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="IVK82_000677"
     CDS             25086..25214
                     /locus_tag="IVK82_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP6391760.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="IVK82_000678"
     CDS             25271..26965
                     /locus_tag="IVK82_000678"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP6391761.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            27032..27238
                     /locus_tag="IVK82_000679"
     CDS             27032..27238
                     /locus_tag="IVK82_000679"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391762.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            27616..28749
                     /locus_tag="IVK82_000680"
     CDS             27616..28749
                     /locus_tag="IVK82_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP6391763.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28780..29100
                     /locus_tag="IVK82_000681"
     CDS             28780..29100
                     /locus_tag="IVK82_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391764.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29129..29593)
                     /locus_tag="IVK82_000682"
     CDS             complement(29129..29593)
                     /locus_tag="IVK82_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6391765.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29725..30978)
                     /locus_tag="IVK82_000683"
     CDS             complement(29725..30978)
                     /locus_tag="IVK82_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6391766.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(31007..31324)
                     /locus_tag="IVK82_000684"
     CDS             complement(31007..31324)
                     /locus_tag="IVK82_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6391767.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31350..31670)
                     /locus_tag="IVK82_000685"
     CDS             complement(31350..31670)
                     /locus_tag="IVK82_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6391768.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32134..33549)
                     /locus_tag="IVK82_000686"
     CDS             complement(32134..33549)
                     /locus_tag="IVK82_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6391769.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33566..34882)
                     /locus_tag="IVK82_000687"
     CDS             complement(33566..34882)
                     /locus_tag="IVK82_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6391770.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35032..35730)
                     /locus_tag="IVK82_000688"
     CDS             complement(35032..35730)
                     /locus_tag="IVK82_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP6391771.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36019..37008)
                     /locus_tag="IVK82_000689"
     CDS             complement(36019..37008)
                     /locus_tag="IVK82_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP6391772.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37088..37921)
                     /gene="msrA"
                     /locus_tag="IVK82_000690"
     CDS             complement(37088..37921)
                     /gene="msrA"
                     /locus_tag="IVK82_000690"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6391773.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38096..39730)
                     /locus_tag="IVK82_000691"
     CDS             complement(38096..39730)
                     /locus_tag="IVK82_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391774.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(39717..41564)
                     /locus_tag="IVK82_000692"
     CDS             complement(39717..41564)
                     /locus_tag="IVK82_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391775.1"
                     /translation="MFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKDNSSILKI
                     ELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSS
                     IFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNICTILI
                     SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLI
                     FNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLV
                     KAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTM
                     GLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWR
                     ILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHG
                     HIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNI
                     TYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKY
                     TPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            41551..43188
                     /locus_tag="IVK82_000693"
     CDS             41551..43188
                     /locus_tag="IVK82_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP6391776.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(43333..44073)
                     /gene="truA"
                     /locus_tag="IVK82_000694"
     CDS             complement(43333..44073)
                     /gene="truA"
                     /locus_tag="IVK82_000694"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP6391777.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(44264..44899)
                     /locus_tag="IVK82_000695"
     CDS             complement(44264..44899)
                     /locus_tag="IVK82_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP6391778.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(45046..46287)
                     /gene="sstT"
                     /locus_tag="IVK82_000696"
     CDS             complement(45046..46287)
                     /gene="sstT"
                     /locus_tag="IVK82_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP6391779.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            46485..47483
                     /locus_tag="IVK82_000697"
     CDS             46485..47483
                     /locus_tag="IVK82_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391780.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGIFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            47496..49514
                     /locus_tag="IVK82_000698"
     CDS             47496..49514
                     /locus_tag="IVK82_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP6391781.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(49574..50290)
                     /locus_tag="IVK82_000699"
     CDS             complement(49574..50290)
                     /locus_tag="IVK82_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP6391782.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(50517..51059)
                     /locus_tag="IVK82_000700"
     CDS             complement(50517..51059)
                     /locus_tag="IVK82_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP6391783.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(51064..51291)
                     /locus_tag="IVK82_000701"
     CDS             complement(51064..51291)
                     /locus_tag="IVK82_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP6391784.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(51370..53250)
                     /locus_tag="IVK82_000702"
     CDS             complement(51370..53250)
                     /locus_tag="IVK82_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP6391785.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(53439..54053)
                     /locus_tag="IVK82_000703"
     CDS             complement(53439..54053)
                     /locus_tag="IVK82_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6391786.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            54195..54755
                     /locus_tag="IVK82_000704"
     CDS             54195..54755
                     /locus_tag="IVK82_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6391787.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(55060..55374)
                     /locus_tag="IVK82_000705"
                     /pseudo
     CDS             complement(55060..55374)
                     /locus_tag="IVK82_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(55628..57263)
                     /locus_tag="IVK82_000706"
                     /pseudo
     CDS             complement(55628..57263)
                     /locus_tag="IVK82_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        17618 a        11526 c         9469 g        18686 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgattg ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacaatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
    27121 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
    27181 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
    27241 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
    27301 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
    27361 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
    27421 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
    27481 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
    27541 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
    27601 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
    27661 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
    27721 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
    27781 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
    27841 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
    27901 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
    27961 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
    28021 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
    28081 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
    28141 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
    28201 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
    28261 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
    28321 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
    28381 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
    28441 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
    28501 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
    28561 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
    28621 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
    28681 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
    28741 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
    28801 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
    28861 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
    28921 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
    28981 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
    29041 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
    29101 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
    29161 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
    29221 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
    29281 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
    29341 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
    29401 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
    29461 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
    29521 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
    29581 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
    29641 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc
    29701 acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat
    29761 cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc
    29821 tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac
    29881 ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac
    29941 actaactaag accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat
    30001 aggtaaccca aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt
    30061 tgttacaacc atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc
    30121 taaagttgca cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat
    30181 aaacggtgcg ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt
    30241 gattgtttgc atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa
    30301 tggtgtgata aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg
    30361 ttcttggacc actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat
    30421 tgcagcaact aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc
    30481 ttttgcaact gccggtggca caccatcagg cattttgatg atcaattttt catttccgct
    30541 taaacgtaca aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt
    30601 tgagctcata cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc
    30661 ggaaacaccg tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa
    30721 gacagaaaga atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc
    30781 accaccaaaa attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat
    30841 tactgctaat gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc
    30901 aactaaatgc cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa
    30961 tgcaacaaat ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc
    31021 gacgatacag atcaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat
    31081 gatcttgact gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg
    31141 tcagcatctc tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt
    31201 tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc
    31261 tttttgcatt tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt
    31321 ccatttattg tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg
    31381 agtacttttt caccattcat catgccatag tcagccatat tgattacatc taaaggaatg
    31441 cctttaggtg ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa
    31501 agtaaaacgt ctacattttt agattctagt tgattatccg catctgaagc agatacagca
    31561 aagatgtctg cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga
    31621 cttgtactca ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg
    31681 ttcctttcta tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt
    31741 aaaaagttga tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa
    31801 agcgcacact aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa
    31861 aaagttgtga cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc
    31921 atctgcggta ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt
    31981 cttattactt gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc
    32041 tgaccctggg tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag
    32101 taaatacaat gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc
    32161 caattctctc ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct
    32221 tcgttcatcg ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg
    32281 tgacacacag tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt
    32341 tgctttgatc aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac
    32401 gattcctttc tcatccatat actgttcttc atattctctt cccataccgt tttctgttat
    32461 cagccaaggg atattatgat aattttcttt tagatacatg gctatatcat acatgatttt
    32521 cggatagatt tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc
    32581 ataatatcgt tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac
    32641 gcgtttagga taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc
    32701 taaatcctct gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc
    32761 ttctgtgtag tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata
    32821 catataagca gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt
    32881 taaaatacag ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc
    32941 cgttgctaaa actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg
    33001 ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa
    33061 ccagtttttt accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa
    33121 atcaactact tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc
    33181 ataatgttcc aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc
    33241 acttatatgc tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg
    33301 agcccaattg atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc
    33361 ctgctggtat tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac
    33421 aaaatgttca tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt
    33481 ccaaccttcc gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt
    33541 aattttcatg atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt
    33601 ttacaaatgg cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg
    33661 caccgatatt tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga
    33721 ccattacaac tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag
    33781 tgataagagg cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac
    33841 caaaaataac tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt
    33901 gtttcatatc ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc
    33961 cagacccgcc catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt
    34021 cttttccagc ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc
    34081 gtatcggctt caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa
    34141 acatcaatag caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga
    34201 cattgtaaat gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag
    34261 ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat
    34321 tcgttggcac ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat
    34381 aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat
    34441 attgtttagc caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa
    34501 cagttggatt aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca
    34561 ctttattcag cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac
    34621 ttagtgtgac atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga
    34681 aacttatctg tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa
    34741 aagcacccgt aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat
    34801 accttaaatt attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa
    34861 aactcattcc agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta
    34921 ttagtcagcg cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat
    34981 aaaacaaaaa ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt
    35041 ttctttttct cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat
    35101 tgcatcaaac gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat
    35161 atcgatcgtc tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga
    35221 ttcattaatg attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat
    35281 cgtatgaaaa tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc
    35341 aatcttttgc gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc
    35401 caaacctgcc atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg
    35461 atcctgatct tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact
    35521 gacaaatgtc cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac
    35581 agcttctcgc accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg
    35641 cagtttatct cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt
    35701 tacttctact agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt
    35761 ttttcgtaat tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat
    35821 taatccgttc atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa
    35881 attcactcct agccttcgat catttttatt ttgaacgaca aaaaatatga tgttcaataa
    35941 cagacgatct tcattcgatt cttgatgtca tcttttgttt tttttaatat atggataaaa
    36001 agtttgatta catcgtttct attctagaat ttggtcgatt ctcaattctt cttctggtga
    36061 gaaatctaaa ttatccatca ttcgaacact ttcttgtaac tgacttaacc gacttgctcc
    36121 gaccagtaca cttgtaacag atttttgtcg caaattccat gcaagggcca tttgcgctaa
    36181 tgattgtcct cgtgatacgg cgattgtttg cagtgctttg attttttcca gagttgatcc
    36241 aacttgttct tctgaaagga atggtatttc cttacgatgt gctctagaat cttcaggaat
    36301 accatgtaga taacgatttg tcagcaaccc ttgagcaagc ggactgaacg tgattgcccc
    36361 tagcccttct tcttctaata ctggagatag atcgtcttct agtagtgcac gagaaaacat
    36421 attgtagcgc atctgatgaa taatgaacgg tgcttccttt ctttttaaaa tttcagtcat
    36481 tttttttgta tcttctccat tatagttgga tataccaata tataatgctt ttccttgtct
    36541 tacaagttgc atcaaagctt ctgctgtttc ttccaaaggt gtatcagggt ctgggcgatg
    36601 atgatagaag atgtcaacat aatctagccc cattctttgg agactttggt cacaacttga
    36661 aatgatggat ttacgagagc cccattcccc atatggccct tcccacatat gataacctgc
    36721 tttgctagaa ataatcagtt catcacggta ggcttgaaaa tcttctctaa aaatacgccc
    36781 aaagtttttc tctgcagctc cagcagggcc tccataatta ttcgcaaggt cgaaatgggt
    36841 aattcccatg tcgaatgcgc ctcttaaaat ctctctttga ttatggatag gatcataatc
    36901 accaaaattt tgccataatc cgagagataa aatcggtaat ttcaaacctg aatgaccgga
    36961 tttacgatat tccatttttt cataacgtgc actatctgca atatacaatt tttctcctcc
    37021 tcatcaaatc tttttaaaac gctttctttt ttctattttt agtttactac attttaaaga
    37081 aaaggagtta atcaaaatga ttttttcgta tatttttttg ccatttgttt tttactcgtt
    37141 gatagatcag aaattgatta cgtgcttgct ggattttttt ataacgtttc ggttgttttt
    37201 tgtaaaattg ctgatgatag ttttctgctg gccaaaacgt accggctggt tcgatttttg
    37261 tgacgattgg ttggtggaaa gttcctgaac tgtctagttt ctgttttgat tgttccgcta
    37321 attctttttg tcgttcgact tgataaaaaa tcactggacg atattgcttc cctctatctt
    37381 gaaattgtcc aaatgtatct gtgggatcag taatttgcca atagattgtc agcaactcgc
    37441 tataacttat ttctcttgtg tcataaatga tctccacagc ttccacatga cctgtgtaac
    37501 caccacttac ttgatcataa tttggttttt caacatgtcc gcccgtgtat cctgacaaaa
    37561 cagataggat cccttttttc gattcaaacg gctcgaccat acaccagaaa catccccctg
    37621 caaatactgc acgttcttga tagtcagcat cttgcatggc atgcttggaa aagtcatatt
    37681 tttgctcctt atcaaatcct tccgtaatca ataaataaaa gtccattaca tcaggcgtca
    37741 gattcattcg gatagctaat ggacgtagtg ttgcttctag ttttgataac tgttctttaa
    37801 cggatatatt ttctcttatg tcattctttg tctgaatcaa tacttttcgt tcccaatctc
    37861 gtgtattcgg attcaaaatc agattataaa gtgtttgaat gaatatttgt tcctcatcca
    37921 tagattctcc tcctttccga agaagacaag ctagtgactt tagctgtaat ttatcataaa
    37981 gtgaggaaat aagcgattac ttcatccccc tacagatttt tcgtataaaa aagagagaat
    38041 ccttatcgac agattctcct ttttaatcaa tttattttat aaatgaaaag ttaatttacc
    38101 ttttatggaa aactatttta aaaaataaac ataaatatgc agttaatgta acaagataag
    38161 aaataatagt cgttactgta taacttgtgc ttactttaat atcagcaact cctttgactc
    38221 cgcttatttg taccaatcca ttagcattca cctgctcctt tacaggttga tgatttattt
    38281 ctacattcgt tcctaaataa gcatatacag gaagtgtgat tacttggctc ttctcattag
    38341 ttactctata tatgatagaa ctaccagttg ttttataagg aaggtttacc cattgactgc
    38401 caatctttgc ttcgtgtttt attatatgct ccttattctc caatgaagcg ataggagaat
    38461 aatcatataa aaaataatcc attcctttag tggtatacgc ctctactgtt tctttgttta
    38521 caacaatatg tcccggctga gaaattattt tttgattgaa atttttttta ctagaaacag
    38581 ttaaagtgat taataacaaa acagtcataa ataaataaaa tagttctctt gtcttattgt
    38641 atcttctatt tttgaaaaat atcatcgtta atatcagaga agagaatatt atttgcaagc
    38701 ttaagatccg ccaaggaaat tgtaacaatt tggctggcgt attttgcaga agataccatg
    38761 gcaccaaact agttgtagct agccaagtca aacacgtacc ataataaatc tttttttcta
    38821 tgtttgttaa ttttttaaat aaaatcaggg gaatacataa agataacaga ccaactagtc
    38881 ccattgagta agtagttaag tcgttgttca tagcttcagt cagactatcg aacacattag
    38941 atgcatgagc atataaattt gtcacgctag gtttgttgat tttttggtag agcatttgtt
    39001 caaacatcgg ataccaaaaa gcactcgtca gaaccaatgt cattcctgcc gcactaaata
    39061 attttttaaa tatagcaata aaatctgcat tcttgaaacc tttagaaacc aaacgaataa
    39121 aaataaaaat gataattaat gctgtactca ttaatgttga tagtacatgg ctgtacacta
    39181 gcaaactcat actcaatgct agctttacag acggatactt ttcttctttt ccagagatga
    39241 tttgataagc ataaaataat acagggataa caagtgttat tgcgatagct tcggcaattg
    39301 cggaacgata gtagatgtct actgtccgat aagcagagaa aatatagaga caactaaata
    39361 aaaatgccgc ttttttttgt tttaaaaacc gctcgccata atgataagct acgagtgttg
    39421 tacacaagtt caataaccaa acgtaaataa tatagcttac aatcaaattt tttgtcatcc
    39481 aatataacat tacagctgga aaaaaagtca gaaagggata aaaataattt acaccatatc
    39541 cagtataatt gaatgtctca taattaatcg gactggtaaa aatagtcgat aaccccttga
    39601 ttcgagataa gtgaaaagaa agatctccag tggtataaat actctgtgta tttttgaaaa
    39661 agatgaaaga atatatacta ctaactataa ttaatccact caatattact acttttttat
    39721 ttattttcat tcttcttgtt tatctccttt ttagtcacat atcgagaatc tttcaactta
    39781 ttaggtgcaa atcgtttaaa acttgaatac accactaaac aataagtcag aatactgatc
    39841 cataaagcca tgagagcagt tggagtatat tttgttgtaa tttctatctt attttctcca
    39901 gcatttacat ccacattcac taagccatgt tttttataag agtcatttac gactttaccg
    39961 tttactttta cctgtgtacc taaataagca tatactggta ctgtcacttt ttgctttttg
    40021 ttgcttgcca ctgtgtaagt aatgttatta tctgcagctt tatacttccc tttttcccac
    40081 gattctccta cttttacttc atgtttttct aagtgaggcc tttcttttat cgcttctgtt
    40141 ggggcgtaat cgtataatcc tcccatatct gatgttgtat aacgtgggag tgtttcttca
    40201 ttgataacaa tatgaccgtg ttttgattgg ataacttttg cgtaattttc ttttgctgct
    40261 atagacgtga cgagtagaaa caccacagta gcgccaatga ttaaccatga atatttttta
    40321 ttcatagtct ttttcataaa aacaagcgtt atgatcattg aagaaaaaaa gatttggata
    40381 cctaagatac gccaaggaaa ctggagtaag cttatcggtg ttttttgaaa caaaaaccat
    40441 ggaaataaat tagttacgca tacccatgta gaaactacgc caacatagat gattttttct
    40501 tttttctcca tttctttcag tatgaataaa ggaaaaatca acgcaaccaa tcctaataac
    40561 cccatagtgt atgttgtaat atcgttattt aacgcttgaa tgatactatc tgaaatagaa
    40621 agtgcttcta gctgcaaatc tgttttgaag ggactgtgta tttcttgata attcatctgc
    40681 tgcaacatag gataccaaaa atagctagtt aatgcacaag tcaaaaaaac agcctttact
    40741 aaattcacga ccacaaattt tatatcgttt tttatttgct tgcttaaaaa caaacgaact
    40801 ataatcatac aagcaatcaa tgcactacac aatagtgtag ttaagacatg agtatacaca
    40861 agtagactta tactgacaga taaagcgatg actttttttt ctctattgaa tatcaatcca
    40921 taagcataat ataaaacagc tgggataatc gtaagagcga ttgcttctgc aattgcggat
    40981 cgataataga tatcaactgt tcgataagtt gcaaaagtat ataaacaact aaataaaaaa
    41041 gcagcatcta tctttttaaa aaacttttct ccataataaa aactaatcag aatcgtacaa
    41101 atatttaata accaaatata tagaacataa cttaatatta agttatttga aattccataa
    41161 aaaacgactg caggaattat tgtaagaaaa ggataaaaat agttcagtcc atctccataa
    41221 ttgttaaatg ttgtatagtt gattggccct tcaaaaatag aagataaccc tttgattcgt
    41281 gaaagatgaa aagataaatc acctgttgta taaatttctt gagcatttct cataaaattt
    41341 actgaatata aagtactaag taagataaga actactaaaa gaaatgcttt tttacttatt
    41401 ttcatagatt tgttgttctc cttttttaat tctattttca gtatactaga attgtctttt
    41461 tttaataagt tttgcgttaa atttttcata gcactagtat gatatactag aaaagaatca
    41521 atttttatta cttttaaata ggagaaaaaa atgaaaaaaa acaaaaacca aataatagat
    41581 atcattttta tgttatttct aagttttgct tacgtcatcc ctctattcat gtctaaggga
    41641 atctatcata gtgtcaatca ggatacttac tttcatttat ctcgaatcat aggactagat
    41701 aatgtatgga gttctccagt aaacttcaat aacttcgatc atcatggaac aatgatgaat
    41761 attttttatc catggcttac tctttatcca gcttttttat tttataaaat gatgggaaat
    41821 cttgttttaa gttataatat ctactatttt tttatcactt ttttaacaat ggtcgtttct
    41881 tatttttcta tgaaacaaat aaaaaacaat cgttatattt cgctgttatt ttcgattatt
    41941 tatacatttt ccgcctatcg tgccattgac atattccatc gtgcttcatt gggagaagct
    42001 gtcgctttaa catttttgcc tttgatacta atggggtgtt acgaaattta tattagagat
    42061 taccaaaaat ggtactggtt gtctattggc atgacgctag tagtgtatac tcatttactt
    42121 tctgtcgcta tggtttctgt atttattgga ggaacgttgt ttctttcttt ttatttttgg
    42181 gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta ccgtcctcac ctttttttta
    42241 agtgcgggat tcttaattcc ttttattcaa caatctagag cacaagaatt gaaagtccca
    42301 ctgggaaaag aattaagtgg tatggctcct agtgatatgc tgacacatat tttaaataat
    42361 aactataaca actatactat tggattattc ttatttctcg gattgattgg tgcttttatt
    42421 tttataaaaa aattaactgc tgatgattta tttattttct ttcttggtgt atttgttctt
    42481 ttttgttcaa ccaatttatt tccttggcaa ctttttaacc atacaccagt taagagtcta
    42541 caatttgtct ggcggttaaa tggtttttct tctttattta tagcctatac gatgagtata
    42601 gtgatctatt atattttttc tactaaaaat aattcatgga aaataatggt ctttacgttt
    42661 cttgcactaa tattgcatct ctcaggagta agcaattcta tccatgctaa caaagattca
    42721 ttaacactta tagcacctga agacgctgtt gctatagctg aaaattacaa tcatactgat
    42781 tatgcaaaca aagagtcaat ctatcatcct gatatgatta ataatgatca atatttacta
    42841 ggtaaccagg aaataaatcc aacaactcat tttatgagta acaaacttac aattgaatta
    42901 gataattcga ataataaaaa tactgtcttg acgacaccta tttatcgtta taaagggcaa
    42961 gtggcttcta ttaatggcaa actagtacaa acaaaattat ctaagtttgg cacaactgaa
    43021 ttaactattc ctccaggtat aaataaagtc gttattactt atcagtacac aaaactagca
    43081 attgcttcaa gatatttatc tatcgttact ttgattttat ttttacttta tcgatttact
    43141 ttttctaaac aaaaacaacc gagaagagaa gtacaacatt ctcactagat ttttatttaa
    43201 gaaagacttt ttagctgtac acataaacag gctaaaaagt ctttttttat atttctttaa
    43261 tttgcttcat acattattta acatcgatat ttatactgtg aaacatgcgt ttcatccatt
    43321 atcaaagtat ttttatcgat aataaacttc atccaaaaac agaccttgtg cagggacggt
    43381 ttctcctgca tgttgccgca ctttgctttc aaatatttca tcgattgttt ctatcggcat
    43441 ggcacctgtg ccgatttcta gtaatgtccc catgataatc cgcaccattt tatataagaa
    43501 tccgtctccg acaaaagtaa aatgaagcat ttctcccttt cgttcgatag ataattcatc
    43561 aatcgtacgc actgtggatt tttttgattt tttcaatgca gaaaaaccaa tgaaatcatg
    43621 tgtacccact aatttttcgc aagctgcatt cagcttgtcc atatccaact cttgagggaa
    43681 ataaaagctg tggtttcgct caaatgctga aggaatcacg ttgttccaaa cataatagct
    43741 gtattttttg cccaccgcat tatatcttga atggaagcgc tcaggcattt cttcgattct
    43801 tttgatgaca atatcccgcg gaaggtaacg attcataaag tcgatcattt ctgccagtcc
    43861 aatttctgtg tttgttttaa aatttgctac ttgtcctcgc gcatgggtgc cagcatctgt
    43921 tcttcctgat ccaataattt ctatctcttc atttgtcatc tgatgcaata ctttttcgat
    43981 ttttccttga atggttttat cgtcatctcc aagtctttgc catcctgaat atctttttcc
    44041 gtcatattcg attgtcaact tgatgtttct catattttag tggctcctct gtcttccatt
    44101 tggtcttctc tctacctttt taagattgta gcacaaaata tagctccttg cttctctaag
    44161 acagaagcta tacaagaaaa ctgtggaaat atatatttca gaaaaacaaa aaacagccac
    44221 cggatgaagt atccgctgac tgttcaattt gacggctcaa gttttactgt ttgagatgca
    44281 agatactcaa ccaggcaatg ggcacataat taaagaaaaa actgattctt tggcaaaaac
    44341 ctattttatt cagtaatggc caagaattta tagaaggaat tcttgccagt ccataaatcg
    44401 ccgcaaaaag caaactgatc acagacagtc caaggtataa gtggcttacg tccatttgcc
    44461 cattttttcg atagatcaag aaaagaagaa ctggaaacaa taaaaaacca ctgtaaccta
    44521 aacctgatcc gatattatgg acccatgtcg agacattcca cgaagattca tgggtattga
    44581 tactgaacaa acctgtaaag atacaatcac ctataccata gaacgcgatg gctgctgtaa
    44641 ctagggtagc taacacacga gatgtactaa caaatgcagc atacaatgca ggcaatgaca
    44701 ggatgaaaaa gatccctgaa accacagacc aaataagaaa cgcttttctt acaggactgg
    44761 aaacctctcc aaatacactg atcacgctct tcatttgatc taattcagga taatacaatc
    44821 ctaatacgta cggagtcaaa aaatcactca gcacgcctaa taataaaaag aaaaaaccaa
    44881 atttacgtaa aaaatccatg atacgcctcc aataacaatt acctatgctt ttaattgtta
    44941 ttataaacga ctttatcttg atttaacaga aacctctatt tacacgaaca tctatataac
    45001 tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct aacgttcaac gttcacttaa
    45061 tccatctaaa caatctttca atttgatttt ttccccactt cttttacgcg cgcctagctc
    45121 tcctattgca gtaaacaaca gatcacttga cgaatttaag gcagtttcca ttgaatcctg
    45181 gataacaccg ataataaatc caataccgac gatttgcatc gcgacatcat tagagatacc
    45241 aaacaaacta catgctaatg gaatcaataa cagtgaaccg cccgcaacac ctgaagctcc
    45301 acaagcagct aaagcagaca aaatacataa aaggactttc aaaacaaaag gtgcttcaat
    45361 ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg gtaatcgcgg caccgcccat
    45421 attgatcgta gcacctaacg ggatcgatat ggaataagac tcttttgtca atttcaatct
    45481 ttcagccaac atcatgttta tcgggatatt tgcggcagaa cttcgggtaa agaaagcggg
    45541 aagtgcgctt tctttcaaac aaaagaaaat caatggatag ggattttctt taataaacaa
    45601 aaacgtgagg aatgggtaca caactaatgc gacaaacagc atcgtaccga tcaaaacaag
    45661 taaaagctgc agatactctg ccaatccagc gattccagtc gtagcaattg attgataaac
    45721 caatcctaaa atccctatcg gagcaaatgc aataatcact tgtacgactt gtgacaacgc
    45781 tatagatatc tgatccacca actcttttgt cgattctgaa cgtgctcgaa aagctagtcc
    45841 caacaataca gcccagaata agacgccaag gtaattcgca tcaataagag attgaacagg
    45901 attttgtgtg acattcgtca acatagtaga taaaacactt tccaaacttt gcggtgcttt
    45961 ttcagatact gtttcttgta aagtaatagg aacagtaaaa attgaactta caataactgc
    46021 tacaagtgca gaaagcaatg ttcccactaa atataagatc aaaataggct tcacgaatgt
    46081 cttattccca attttatgct tggcaattga cgccatcgtc aagaaaaaaa ccagaatagg
    46141 tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat attgaaataa atgtccatgt
    46201 ggggaaaaag ataccaaaca atattccgac taaaatacca acaacaatac gttgaactaa
    46261 cgacatatcc aaaaacttct ctaacaaatg accacccttt cacaatttta acaatcattg
    46321 tatcatttta tgcaaagaaa ctaaaggtat aattttctaa aaattctaac aatacaaaca
    46381 accatttctt gctaattttg tactttttct gtctacacgt tgttttttga gatattctaa
    46441 gttaggatgt tattaatggg caacttatca ggaaacgttt tcttatgttt ttgtccaatt
    46501 tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga gagttatggc ttcctgacaa
    46561 tctttgtcgc ttgtataatg atggttttac agcgtatatt gatttcagca gtcactattc
    46621 ctacatatgc aacaaaccct ggtcgattct acatctatac atttttacaa acttccgttg
    46681 ttattggtgc aaatttgatc ccactttaca tgggttacca atatcaaaaa atcgcccgtt
    46741 taaaagtttt acattattta agtcgttttg cctttatatt cctaatagcc acactagttt
    46801 gcaatatctt tttttacgta caagctggca gcttgaatat tcgtgattat tggcttatcc
    46861 taacacctat cagtcagaat tactttactt atgctgtttc ttgtactttg cttttttgta
    46921 gtattcctta tacagttgga tttttaaata aattatcgaa aacaacaatt aaatatctac
    46981 tattattttt aacaattgga ttagttggct gctctactct atttaataaa gacccgtttg
    47041 cattacagaa tggacagagt attttatgga ttttttattt atttttaatg ggttatggtt
    47101 tgaaagaatg gaactggaaa gaaaaggtcc gctataagct tcctcaagta tttatcagct
    47161 tgattgtttt attcggtttg attattttga tgacacaaat ttcactcatc atcagaggaa
    47221 atacagatac agcgctgagg ttttctaaac catattctct cttcgcaatg tattatactt
    47281 tctcttcgtt tttattttta gaaatgctat ctgataaatt aggtataaaa ggaagaggaa
    47341 tttttcttgc tgtatttttg attgtcactc aagttatatg taatttccct ttagtttctt
    47401 atcgcgtatc tacatttatg aaacgtgaat ttccaaactc agggaaggca tggctgataa
    47461 atatcgggca gttttttatt tgatatttat tagcagtgtc cctcctagtt atctttttat
    47521 tttatataca aaaaatccgt ttcattcatc gtttcatcga atacttcgct tttgattcga
    47581 ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg gctttttgtt cataaaagtt
    47641 attttttagt tgcagcattt ttttactgct ttaccttcgt gcagattttc gttttagaac
    47701 caaaagaagg ttggaaagaa actatccagg ttgccttaaa aatatttact cagcgacaag
    47761 cacccatgat cctgacaatc atcattatta tgggattttt cttcttacta ttgttgattt
    47821 ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat taacttactg ctcactattt
    47881 cgacagtaat caaaatggaa atgcgggaag agcctgtctt tccttcagat ttaaaaatgc
    47941 tgaccggatt gtcagaacta ttatcgatgg ttagcccagt cttattaatc gtgggcggat
    48001 tgatccttct cctattattg attactagta ttattgtaca acgtagacta caaaaacagt
    48061 attccttgaa gattcattgg aaacgtcgat ttatcagtat cgctgtatta ttaggatgtt
    48121 tttcgggtgt ctttttcatt aatcacaaaa attcgccttc tttcttgctg tttaatttat
    48181 ttaaagtaaa caagactttc tttaaccagc aaggcgccgt aaaagataac ggaccagtca
    48241 ttcaattcct aaacaatatc gacattaaaa tcatggaaga acctgctggc tattcgaaag
    48301 aaaaaattaa tggaataatg aaaaagtaca acacaaaggc agatgaaatc aaccaaacaa
    48361 gaagtgactg gttaaacaat cagacgatcg ttctcaattt aagtgaaagc ttcagtgatc
    48421 ctgcacgtgt ccctaatctg acagtaccta cagaccctat tccttacatc aaaagcatta
    48481 taacagaagg aacaggagga ttgatgttat ccgtcggtta tggtggcgga acagctaaca
    48541 tggaatggga aggactgaca ggcttgagta tcagtaacct gtctgcttcg ttagttacac
    48601 cttataccca gctagtggag cgtcagaaaa taagtccaaa tattaccgat ctattcgatg
    48661 aaaaaatagc catccatcct tttacagctg ctctttatcg cagaaaacaa gtgtttgata
    48721 aatttggttt tgacaggttc tattatttag acagtccgga taaattaact tatacggata
    48781 aaatagaaca aagcccacgc atatcggatg attcagcata caaagaaacc ttgaaagctt
    48841 tacaaagcaa tacggaaacg tcacaattca tccaattatc cactatgcaa aaccatatgc
    48901 cttatgatga ttactatagt gaattgaact acactgctga aggaactgct gtcattccta
    48961 atagaagaaa agaattacaa acttatatgc aaggccttca ctataccgat acagcagtaa
    49021 aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat tacctttgtt ttttatggtg
    49081 accatttacc cgcaatctat agtgggaatg attcgaaaaa atatggatta gaacagcacc
    49141 agacagatta tttcatttat aacaacaagt atagtcgtga acaagcagct acgttaaata
    49201 aagaagttgt tgctcctaat aactttggtg ctatggctct tgagcatgcg aatatcaaag
    49261 taactcctta ctatgcgttg ttgacagaag tatctaatca tctacctgca ttaacaattg
    49321 atcctacaga aagcttgtct aatcgtttta atggaaaaca ggtctttgtt acagaggata
    49381 gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa agaaattcta gaagattatc
    49441 gtctgataca atatgatctt gttgcaggta accagtacag tgcaacatgg gcgacacaaa
    49501 aaataaaaga ctaacaaata aactgagcaa ggggttgtga taaaagacat gtcacaaccc
    49561 cttgtttatc tcattaattt gcttgtccat aaacgatttg attaaagcca tttttcaatt
    49621 cattattcgc tgtctcgttg aagtaaagat aattatcggg attcccctga ttttgttgtg
    49681 tcaccaatac ttttggctga ttttgctgaa acccgaatag atagacgtgc tttttcagat
    49741 atggttctgt ctctgcatcg gaatagacgg ctgtgatttg atagtctgct tgacctgtcc
    49801 cactttctga ccattctgca gaaacaggca ctccaccaat agccatcttc cactccccgt
    49861 tcaaaatagc ttgagggact ttaagaccat ataagtccac actcatttga ttcgtataac
    49921 ttttgtattg ttgtcccatt gtttggcccc atttggtcat aaatgcctct aattgtgcgg
    49981 atttgtctgc attccacagt gtttgttcaa cttgttcgct gctttctgaa gaggaagatg
    50041 aagcctctac agaagaactt gaagtagatg tttgagttgt ttcagtcgtt ttggaagact
    50101 ctgacgaagt agaggtatct gctgtttctg tacttgctgc agttgacttg ctctctgtgc
    50161 tagttgttgt agtagttgca actgttgctt tcgctgtttc ttttgttgat tcttttttcg
    50221 attcattccc gttggtacaa ccagctaaca aaaaagctag accaattgtt actaaaatcc
    50281 tttttttcaa ttaaaaaact cctttcgatt actcccactt ctttattcta tcaaataagc
    50341 aatgtgattt tattgatttt tcgtattttc tcatcaatct ttcaaatctt ctaaaaactg
    50401 agaagcaccc gctgatgttg tctgtaatac atcagcgggt gcttctttta ttttaatctt
    50461 cgtgattttc caatttacct cacgctttat atttgtattt gaagaaacat agacttttag
    50521 tcatcatcgt cttcctcatc caaatcttgt cggacagttt ttccttggag tctttgtact
    50581 tggtagatga tttctttatt gaaacttaaa agttcaacaa gcaattgttg caagccatat
    50641 ttttcttctt tttcagctaa tttgattgcc cgtgtgataa agacattttg gaaatcaaaa
    50701 tcttttgcaa agttttccaa cattgttgag gctggtaaat atttgttttg accatgttcg
    50761 taaatttttc cataacgcat gacttcttct aaagtagacg gaacgatttc attttcaatc
    50821 actaactcat gagagatttt attgaaccat ctcgcttctt gatcagcata ttctgaaaaa
    50881 agggtactta attcttgcga agatgtaccc aaaacataca attgtgcttg gatcaatttc
    50941 atttcttcta atttaaaatt tgctaataca tgccccacca tggcgccagc agttggttta
    51001 tgatggtcga tatctgcttg ttttgtttct tgaacaaatt tttcttccac tatattcatt
    51061 gatttacgcc tcttttactt ttgctgattg gacttcataa cctaattttt ccacaactgc
    51121 agctaattgt tcagggtgga cttcatctgg atcaaagttt gctttgattt tgctcgcatt
    51181 aaacaaaact ttcgtattgc ttaccccagg ctgttgagaa accgcatgtt cgattttttg
    51241 catacagctt ggacaagcta gtgtatctaa ttgcataata atttttttca tcgtagttct
    51301 tccttccttt ttcttgatag tcttattcta gattacagct tccataaaaa aattgattct
    51361 tatcaagttt tacgattttt cttctttgct ttcgtgtcca aaatacaata aacgcatcgc
    51421 gttaaagata acgatcaaga tactagcttc gtgaacgaac atcccactag ccatgtaaat
    51481 aaacccggca aataagccgt tgagtaaaaa taagacgaca ccaatggcaa tcactacatt
    51541 ttcacgggta tttaaaacag ttttcttagc caagcggtag gcatgaacca acgattcaaa
    51601 attagattgc atcaagacaa cgtcactcgt ttcgatagca acatccgttc ctgatcccat
    51661 agcaatcccg atatcagccg cagccaaaga gggagaatca ttgattccat ctccaataaa
    51721 agcaacgcgc aatccttgtt cttggtattt tttcacaaat tgaacctttt cgtctggtaa
    51781 taattcagca tggacttcat caatgcctaa catatcagct acataatcag cagtcatctg
    51841 attatcacct gtcagcatca ctaactgttt tgctcctgct tgtttcaact tagctaattg
    51901 ttcagcgact tctggtcgaa tcacatctga cacacctatg atttgagtga tttttttgtc
    51961 gattgctaca ataatgaccg agctgcctgt ttgttgtaat tgatgtaaat cttgtacttg
    52021 ctttggtgtg agagaaagtt tgtgagcggc aatcaatttg gtgttgcccg cacaaactgt
    52081 atgaccatca atctcagctg tgattccttg tcccttgatt gtctgattat ctttgactgc
    52141 taattgttga aaatcaatgt ctttctcctg agcataatgg atcacggcgc gccccagagg
    52201 atgatcggat aacgtttcta atcgagccac taaagctaag ctgtcccctt catcctctgt
    52261 atagttcttg atagtggata cagcagtagt tcctttggtc aatgtaccag ttttatcaaa
    52321 gacaaaagta tcgattttgg aaaaagtatc cattgcttca ccgcctttga ctaagatgcc
    52381 ccgtttcgct ccattaccga ttcctgctac attcgaaaca ggcgcaccaa tgactaaagc
    52441 gccaggacaa ccaagaacca aaacagtgat tgctaaacga aaatcacgac taatcagacc
    52501 aacaaccaac gcaataagta aaacggttgg agtataatac tgagaaaatc gatcaatgaa
    52561 tttttccgca tgggatttgg tatcttgtgc ttcttctact aattcgataa ttttggcaaa
    52621 ggtcgtatca tctccgacct tagttgcttc aatcgtcacg tagccatctg ctaaaatcgt
    52681 tccagagaag acttcatctc cactgatttt gacgacttgt cgtgattcac cagtcactgc
    52741 ggcttcatcc gcataccctt tcccgtcaat gactcgtccg tccacaggaa tagctccacc
    52801 agtttttact aagacgatat cgccttcttc cacgtcatcg atttccactt cttcagtcgt
    52861 tccatcttct tggacaacac tagcagtaga tggggccatc tcggtcaaag ccttgattga
    52921 ctcccgcgtt ttggtcaatg ttttttgttc cagatagcta ccaaacaaaa acaagaatgt
    52981 aacgatagct gactccttgt attctccgat cataaaggca ccaagcacag caatagtaac
    53041 gagtaaatca attgagataa ctttgacttt cactgcctga tacgcatgga tcataatagg
    53101 tatgaccgca ataatagaag ctatggctag agcaatgtta tagaacaaga caaaaccagt
    53161 aaattttccg ataaaaccaa aaataatcaa cacagctgat aaggcggtga tatgatttcg
    53221 atgctcttgg atccactttt gtaatttcat ctttctccct cttttccttt cattcttacg
    53281 agactaagta tagatactta tccatcataa aaaattgacc actatcaagt ttagtcaaaa
    53341 aggcggtttt cttttggtag aatacactac aaaaaatagt tagcaagaag actcactaaa
    53401 agtgatcttg ccaactattg tcgtttctat cgaaattttc attgaatgat tttcctgtac
    53461 agcatacatc ggagatctat ttctttatct aagattttta tcttgctaat tttttccttt
    53521 agtctttcaa atccacgtcg ttcatagaat tgatacgtac agccactatc tgtaaaaagg
    53581 aatatttctt ttcctttctc tcttttttca aattctgtta gcaacttggt accgatgcct
    53641 tttgttttca aatcaggatt tgcagctaaa aaaatgatct ctccatctgg cgaatgcttc
    53701 tttaaatatg ctgcaagcat ttgttcattt gctgtctcgt aagctccttt actttcttta
    53761 gcaaatatat tttgtaaaaa atcgaatatc ttaacatata tttgctgtcc aaaagaacga
    53821 tgtttctttt tttctccttt tatctccgcc agcaaaacac ctgccaactc gtcctctgca
    53881 tacaaagcaa gaatttgagt tgctcgattc aactccatat acaaaaaata tcttccgtaa
    53941 agattcagca gaagttcatt ttccatatat ctctcaaagt tcattccttc aacagcaaaa
    54001 tagatagctt ttttgtaatc tttcttttct aagggtttaa aagttagttt catcatttcg
    54061 cctcctcaac ttcaatgatt gtgttatact tacattataa tagacacagt gtctactatc
    54121 tataatcaat catctataag tttgtcgatt aagcgacagc tttgctttga tatgtcggat
    54181 aaggagatag gtaaatgaaa aagacagata gacgcgtcaa aaaaacagaa aaagctttgg
    54241 ctgaaacact atccacatta ttagtaaata aaaagattca agcaatcact atacgcgaac
    54301 taacagaaac ggcagatgta catcgttcga ctttttatac acattataag gatatctatg
    54361 atctatatga tcaattagaa agcaactttt ttcgggatct aaatgaaatt ctctcttatg
    54421 atccagccca tagctatgag gaactataca ctcgcttgat tgactatgtt tacgcaaata
    54481 cttttttata tcgcttattt atcctcaacc atggtgatat ttcgtttcag aagaaagtta
    54541 aacaagttat tgaagaaaac tatctgaaca tatggctata tgaagacaag aaaacgatta
    54601 tcacagaaga aatgagattc tttactacgt accacgtcca aggttgtcta tccgttctca
    54661 atcggtgggt agaaagcaat tacaagtact ccaaaaaaga attattagaa ctcttacaga
    54721 aactgaatga tcacatagag aaaatcatgc cttgaataaa aacatactaa gcttagttaa
    54781 gaagaaccgt aaccttctcg tataacaact gaatcttatt tagccaaatt gtttactgaa
    54841 gattttttga gtaaaatttc tataattcct acttattgaa aagtagtttg tagtgctgct
    54901 tctccctctc taagtgatat atcttgttgt accaatcaaa ttcacctcat cattaataaa
    54961 ttacttttag gctaatttat tacaacttat aggttgaagc tgtaaccaat ttatagtcat
    55021 attatatttt tatcatccag tttattgaat tcttctcgtt tacctcaatt cattttcaag
    55081 gggaatcagc tcatcatcta acattttacg ccatgctcgc ttcatttctt gtgggtattt
    55141 aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac tgttgataat
    55201 tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag aagcatgtaa
    55261 ttttttctca gaaacaaacc actcattgga aaagacatga atcaaatcat cacttacttg
    55321 tgtaaaatag cgacgtttaa tgacaaagat atcttcattg ctatctagtg taccttcatt
    55381 tctttgtgct taaattcaaa gataattttc tcaataaaga tttttgattc cagtaagatt
    55441 cctttactcc ttttacaaca tagcgttgtc ctttgttttg aaacatcttc ttcccctctt
    55501 tgccattaaa aatagcccaa ttctctataa agaagattaa gcttcattat tataatataa
    55561 agtttaaatt tggtttatta cttacttttt cttattctct agtaagctaa tattgaaaaa
    55621 aatgtaatca gatcattatt tttatatgtt gtaacttcct gataagaaaa ctaattaaaa
    55681 gaaagaggta actacataaa ctaattaaaa aagctaactt catagttgga ggataaatgg
    55741 aagaaacttc aatgattgtt tctccttgtg ttccttcaat cgacactagt cctattatca
    55801 atctctcttt tttgtccatt tacttttgcg actgttcccc aataatcaaa tacaggaagc
    55861 gttatttttt gtcctttaga ttcatgtact gtatagacta ttttcgaatc catcacttga
    55921 taaggcatag tatgccattc cttgccaatt cttacttcat gattctgcaa atgttctttg
    55981 tattgtaatg cttccgtggg tgcatagtca taaagtcctc cgggaaggag tgtcgtgtaa
    56041 cgaataagat tttcttgatt cattaccaag tgatctctct gagtgacaac tttatgttca
    56101 tagtttgctt ttgttgcagc agatacagcc aacagtaata aaacaattgc cgttatatta
    56161 actacttttc tttgcttagc tgatgtgctc ttcatgaaaa cgaacgataa aatcaacgag
    56221 ccaaacagta cttgaaagcc taagattcgc caaggaaatt gaatcatttg aatgggtgta
    56281 ttttgtaata aagaccatgg aaaaaatttg gtggataaga gccaagagac taccgcacta
    56341 aggtaaatca ctttttcttt gcgattaaat cctttaaaaa agaagagtgg acagatcaaa
    56401 gaaataatac caatgattcc taccgtaaag gttgtcaggt cattattcat cgcaccaatc
    56461 aaactatccg agacatttaa tgcttggtgt tgtaaggtcg ttttatctgg ctcattaatc
    56521 gattgaacca tgatttgtcg gaacatcgga taccaaaagt atgctgttaa aagaatcgtc
    56581 attcctactg cctttgacaa ctccacaatc aaccggatgc tttctttttt tcttgtttca
    56641 cttactacaa agccgcctaa gactagtaag ccaacggcta ttacactcat aaaagtggaa
    56701 agaacatgtg tataaatgag taagctcata ctgactgcta atggaattgc acatggcttt
    56761 ttctcataaa tcagtgcgta agcataatac ataactatcg gtataaccgt aagtgcaatc
    56821 gcttcagcaa tagcagaacg atgataaata tcaatcgtac ggtacccata aaatgtatat
    56881 aaacagctaa atataaaagc cgcatcgatt ctttttaaaa attttagtcc ataatgaaac
    56941 atcagcatga tagtacaaac attcaatagc caaacatata agatataact aacgattaaa
    57001 ttattactta tccaataaaa aatgactgcc ggaaaaaagg tcaagtaggg ataaaaataa
    57061 ttcactccgc tcccataatg attaaacgtc gtaaaattga ttggaccaga aaaaattgaa
    57121 gataaccctt tgactctagc aaaatggaag gcaatatccc ccgatgtaga aatagtatga
    57181 acatttctaa aataattgat tgaataaagt ccagcaataa ttgctaggag aatggacaat
    57241 attatttttt tctttacttg cattacttaa ttatatactc ctttcatgga ttgctttat
//
LOCUS       DACCCS010000011        56802 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.011, whole genome shotgun sequence.
ACCESSION   DACCCS010000011 DACCCS010000000
VERSION     DACCCS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.011"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            100..570
                     /locus_tag="IVK82_000707"
     CDS             100..570
                     /locus_tag="IVK82_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP6391788.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="IVK82_000708"
     CDS             792..1895
                     /locus_tag="IVK82_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP6391789.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            2116..3075
                     /locus_tag="IVK82_000709"
     CDS             2116..3075
                     /locus_tag="IVK82_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6391790.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            3245..3925
                     /locus_tag="IVK82_000710"
     CDS             3245..3925
                     /locus_tag="IVK82_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP6391791.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            4015..5049
                     /locus_tag="IVK82_000711"
     CDS             4015..5049
                     /locus_tag="IVK82_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP6391792.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            5063..5398
                     /locus_tag="IVK82_000712"
     CDS             5063..5398
                     /locus_tag="IVK82_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391793.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            5382..7862
                     /locus_tag="IVK82_000713"
     CDS             5382..7862
                     /locus_tag="IVK82_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391794.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            7918..9477
                     /locus_tag="IVK82_000714"
     CDS             7918..9477
                     /locus_tag="IVK82_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6391795.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            9474..10838
                     /locus_tag="IVK82_000715"
     CDS             9474..10838
                     /locus_tag="IVK82_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6391796.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            11009..12079
                     /locus_tag="IVK82_000716"
     CDS             11009..12079
                     /locus_tag="IVK82_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6391797.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            12094..12762
                     /locus_tag="IVK82_000717"
     CDS             12094..12762
                     /locus_tag="IVK82_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6391798.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            12782..13360
                     /locus_tag="IVK82_000718"
     CDS             12782..13360
                     /locus_tag="IVK82_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6391799.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            13761..14672
                     /locus_tag="IVK82_000719"
     CDS             13761..14672
                     /locus_tag="IVK82_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6391800.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(15050..15967)
                     /locus_tag="IVK82_000720"
     CDS             complement(15050..15967)
                     /locus_tag="IVK82_000720"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP6391801.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            16169..16606
                     /gene="zapA"
                     /locus_tag="IVK82_000721"
     CDS             16169..16606
                     /gene="zapA"
                     /locus_tag="IVK82_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP6391802.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            16664..17212
                     /locus_tag="IVK82_000722"
     CDS             16664..17212
                     /locus_tag="IVK82_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP6391803.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            17327..19687
                     /locus_tag="IVK82_000723"
     CDS             17327..19687
                     /locus_tag="IVK82_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP6391804.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            19774..20088
                     /gene="trxA"
                     /locus_tag="IVK82_000724"
     CDS             19774..20088
                     /gene="trxA"
                     /locus_tag="IVK82_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP6391805.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            20270..22048
                     /gene="uvrC"
                     /locus_tag="IVK82_000725"
     CDS             20270..22048
                     /gene="uvrC"
                     /locus_tag="IVK82_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP6391806.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            22193..23092
                     /locus_tag="IVK82_000726"
     CDS             22193..23092
                     /locus_tag="IVK82_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6391807.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            23085..24347
                     /locus_tag="IVK82_000727"
     CDS             23085..24347
                     /locus_tag="IVK82_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6391808.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            24508..26406
                     /locus_tag="IVK82_000728"
     CDS             24508..26406
                     /locus_tag="IVK82_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP6391809.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(26736..27455)
                     /locus_tag="IVK82_000729"
     CDS             complement(26736..27455)
                     /locus_tag="IVK82_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP6391810.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            27792..28124
                     /locus_tag="IVK82_000730"
     CDS             27792..28124
                     /locus_tag="IVK82_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391811.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            28111..30114
                     /locus_tag="IVK82_000731"
     CDS             28111..30114
                     /locus_tag="IVK82_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP6391812.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            30133..30603
                     /locus_tag="IVK82_000732"
     CDS             30133..30603
                     /locus_tag="IVK82_000732"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP6391813.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            30624..31208
                     /locus_tag="IVK82_000733"
     CDS             30624..31208
                     /locus_tag="IVK82_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP6391814.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            31223..32209
                     /locus_tag="IVK82_000734"
     CDS             31223..32209
                     /locus_tag="IVK82_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP6391815.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            32199..32510
                     /locus_tag="IVK82_000735"
     CDS             32199..32510
                     /locus_tag="IVK82_000735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP6391816.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            32532..34313
                     /locus_tag="IVK82_000736"
     CDS             32532..34313
                     /locus_tag="IVK82_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP6391817.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            34306..35682
                     /locus_tag="IVK82_000737"
     CDS             34306..35682
                     /locus_tag="IVK82_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP6391818.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            35689..36324
                     /locus_tag="IVK82_000738"
     CDS             35689..36324
                     /locus_tag="IVK82_000738"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP6391819.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            36354..37709
                     /locus_tag="IVK82_000739"
     CDS             36354..37709
                     /locus_tag="IVK82_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP6391820.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(37901..39295)
                     /locus_tag="IVK82_000740"
     CDS             complement(37901..39295)
                     /locus_tag="IVK82_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP6391821.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            39387..40358
                     /locus_tag="IVK82_000741"
     CDS             39387..40358
                     /locus_tag="IVK82_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP6391822.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            40355..41137
                     /locus_tag="IVK82_000742"
     CDS             40355..41137
                     /locus_tag="IVK82_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP6391823.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(41306..41677)
                     /locus_tag="IVK82_000743"
     CDS             complement(41306..41677)
                     /locus_tag="IVK82_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP6391824.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            41811..42254
                     /locus_tag="IVK82_000744"
     CDS             41811..42254
                     /locus_tag="IVK82_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP6391825.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(42297..43106)
                     /gene="yidA"
                     /locus_tag="IVK82_000745"
     CDS             complement(42297..43106)
                     /gene="yidA"
                     /locus_tag="IVK82_000745"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP6391826.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(43125..44492)
                     /locus_tag="IVK82_000746"
     CDS             complement(43125..44492)
                     /locus_tag="IVK82_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6391827.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(44476..45306)
                     /locus_tag="IVK82_000747"
     CDS             complement(44476..45306)
                     /locus_tag="IVK82_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP6391828.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            45469..45906
                     /locus_tag="IVK82_000748"
     CDS             45469..45906
                     /locus_tag="IVK82_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP6391829.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            45982..46602
                     /locus_tag="IVK82_000749"
     CDS             45982..46602
                     /locus_tag="IVK82_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP6391830.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            46791..47297
                     /locus_tag="IVK82_000750"
     CDS             46791..47297
                     /locus_tag="IVK82_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP6391831.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            47314..48213
                     /locus_tag="IVK82_000751"
     CDS             47314..48213
                     /locus_tag="IVK82_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP6391832.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            48300..49184
                     /locus_tag="IVK82_000752"
     CDS             48300..49184
                     /locus_tag="IVK82_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP6391833.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(49376..49447)
                     /locus_tag="IVK82_000753"
     tRNA            complement(49376..49447)
                     /locus_tag="IVK82_000753"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49412..49414),aa:Arg,seq:ccg)
     gene            49507..50841
                     /gene="rpoN"
                     /locus_tag="IVK82_000754"
     CDS             49507..50841
                     /gene="rpoN"
                     /locus_tag="IVK82_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP6391834.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            51044..52081
                     /locus_tag="IVK82_000755"
     CDS             51044..52081
                     /locus_tag="IVK82_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP6391835.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            52122..53123
                     /gene="gap"
                     /locus_tag="IVK82_000756"
     CDS             52122..53123
                     /gene="gap"
                     /locus_tag="IVK82_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP6391836.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            53233..54423
                     /locus_tag="IVK82_000757"
     CDS             53233..54423
                     /locus_tag="IVK82_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP6391837.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            54527..55282
                     /locus_tag="IVK82_000758"
     CDS             54527..55282
                     /locus_tag="IVK82_000758"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP6391838.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            55398..56696
                     /gene="eno"
                     /locus_tag="IVK82_000759"
     CDS             55398..56696
                     /gene="eno"
                     /locus_tag="IVK82_000759"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP6391839.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        18898 a         9877 c        12010 g        16017 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatact tcgccgattg taaaattaag
     2041 ctagacaact aaaaaaagtc atttgtgcta cactcaaaat agttccgacg aaagaattat
     2101 aaggagtgag tacaaatgac ctataaccat cttacaccaa ctgaactcgt aatgatagaa
     2161 gcttatttca atcaatcaca acctgtttca aaagtagcca atctgttaca acgttctcga
     2221 caaactattt ataaggtcta tcgttttctt aaatctggtg gaagcgcaat gagctactat
     2281 aaacaatata agaaaaacaa acgtaactgc ggaagacatc cgattgtttt acctgatgag
     2341 caacaggaat acattcaaaa aaaggttgtt caagggtggg ctcctgatgt ccttattggt
     2401 cgagcagagt ttcctattgc ttgttcggta cgcacactgt atcgaatgtt taaagataaa
     2461 acctttgaca ctcacgattt accaatgaag ggaaaaagaa aaccaaacgg tcataaagaa
     2521 aaaagaggaa aacaagggtt tcgccggtca atccgagaca gaaagacaga ctatacccag
     2581 tttgatcatg aatttggtca cctggaaggt gacactatcg tgggtggaaa acataaaagc
     2641 gcggtcatca cattagttga acggctatct aaagtcatca ttaccttgaa acccgaaggc
     2701 agacaggcta aagacattga aaataggctg aatcaatggt ttcgttgtgt cccaaaaaac
     2761 ttatttaaat ccatcacatt tgattgtgga aaagaattct caaattggaa aaagattagt
     2821 aataccaacg atatttcaat ttattttgcg gatccaggaa caccttctca acgaggattg
     2881 aatgaacatt caaatggctt attacgtaaa gatggtttgt ataaagctat ggattttaat
     2941 ggtgtatctg aaacctttat ccaatcggtg gcatcgaaac gaaatcatat tcctagaaaa
     3001 tcattacact atcggacacc aatggaagta tttttgagtt acgtcacaaa agatgatttg
     3061 tctaacttaa tttgacaaac gaaacttaat aaaaaaggaa gaatattcat gtaaacgatt
     3121 ttattgtttt tttctctttt atacaaaaag ggtcataatt tcaagtaagc ggatgaattc
     3181 atgaggattt tttgcatact gtctctacta tacttaggtc actaagaaaa aggagtgatt
     3241 tattatgaaa aagaacatga ttgggacatt attactttca acacttcttc taggcggctt
     3301 ggcaacacca gcttttgctg agggtcaagc gacttcaaaa ggagatatta cctttactga
     3361 accaacaaat accgtggagc ctcttaaccc tactgatcct tcaaaaccag tagagccagc
     3421 agatccggaa aatccagcaa ctggccagac aggtccttta acactggatg tagttcccga
     3481 attgccattt ggtacacacg agattgaaag cggaacaaaa acgtatcaag tagacgcttc
     3541 aaaaaacgat acaccttatc tccaagtatc agatcgtcga ggtgtaggtg cagatggcca
     3601 agcacaaggg tggaacgtga cagtttcggt ttcagatttt gtaaacggtt cacaagtttt
     3661 gcaaggcgct gaattagatt ttggaacatc cactgttaaa agtacgtcag ataacgaatc
     3721 cactggacca actagtcaaa ctgtaactgg gttgagtaaa gcaagtgcag cgacaccaat
     3781 atttacagca gcaaaagatc aaggtctggg aacatggctt tctgtttatg atccagcaaa
     3841 tatcacactg aaagtgccga aagcagcagc tggaacattc acggctgatt tgacttggaa
     3901 cctcgtggct ggcccagtag catagagtat gaaataaatt caacaaactg cggtgaaaat
     3961 actataggtg aaggattttt taccgcagtt gttgttgaaa ggatgtggtg gacaatgaat
     4021 caaagtaaac gactgtttgt ttcgtttttt tctattttaa gtctattttt catttttccg
     4081 tcaatcagcc aagcaaagga ttcagcagga gactttggaa tcaaaccggt atttccagag
     4141 aaccaaatag ataaagcaat cggttatttt gatttattag tggcaccgga acaaaatcag
     4201 acactagaag taattatcag caattcttca gatgaagaac gaacatttga ggtctctgtc
     4261 aatccggcgg taacgagtga tggtggaacg atcgattata gtcaaaaaaa cccaacgtta
     4321 gatgagacat tgccgtttga tgtccgtgac gttttgctca ttgcgaaaaa ggaaatcaat
     4381 gtatcagcac atgcagaaac aactgtacct atagaagtga aaatcccagc aaaaagcttt
     4441 aagggacgtg tattagcggg tattcatgtt tcacctaaag aagaagctga aacagaaaat
     4501 gctacagaag gagcacaaat caaaaatcgt attgcttata atctcgcagt cgttttacaa
     4561 gaaagtcaag agacgataga accggatctg aaacttttat caggagattt ggacgaggtc
     4621 aacgcaaagc caactgttca gcttcgtttc caaaatcctc agcctaggat tatcagcaat
     4681 ttgatattta cgagcaagat cttttatgaa aatcagcttt atattgaaaa tacgtctaat
     4741 gcatttttag ttgcaccaaa ttcgaatttt cacttgaatc ttgatttagc tggtgataaa
     4801 gcaaaagccg gcgattaccg cgcagaaatc atagcaaaat caggcgacag taatgaatgg
     4861 cgatttacac aaaactttac gatcaaaaaa gaaaaagcac aaaaagtaaa tgaaaattcc
     4921 gtttttgcag tacaagaaca atctttccca tggctttatg caggattggt tgctgcagtt
     4981 ctagtgttat ggttgatttt gctgtttttg tgggtccaca agaaaaagaa aaaaggagaa
     5041 acaacatgat gaggtgattt cgttgaaaac atcgatctta ttgggaggga ttattgcaag
     5101 tttgctgttc ttcagctggc aagagccagt ccaagcagat accaaaagtt caggtacgat
     5161 tacattttat gatctaagtc caattccgca tccaagtggt tcagtcaatg gcgcatcaaa
     5221 acagcagaca cagctaccga tttataaaga atctaatcaa catggcgtat tcccggagac
     5281 gaatgaacaa aagatccgtt ttctttattt gatcattggt atagaattat ggcttcttgc
     5341 gtggttgttg ttatggagaa gaaaggaaaa ggaggatgaa catgttgcta caaaataaat
     5401 tttcattttt tcgctccttc ttttttactt cgctttttgt tttaagtata tttatctttt
     5461 caccttcggc agaagctgtt ggagcaacgc ctcctgccat ttcttatgat ccatacacgt
     5521 atgaaaatat cgtacctcca aatggtggac gtagtgcagt caatggcggg aagcttgtcc
     5581 aactaactgg ggctaaccaa ttgcctaaat tgactgctac ttatttgaac gatggttcaa
     5641 atgatccttg gaaaggtggc acggtccgtt ttcattggga aagccatgtg aaactaacaa
     5701 atgtacttga tttggtgaat atcactgcac aaggatttgc atttgtggcg atcatcaatt
     5761 tgccggaaca tgtatcaagc agtgatgtat taaaagcaat tgactggaac aacgcctact
     5821 tgtatattgc cggtgccccc atcaaggtta ctagcggaag tttattatca gcaggaagcc
     5881 ataccttgcg ttttgggatg gggtcgtggg atacaaatac tctattgaca gatattattg
     5941 ctattattac gcgaggcgaa ttcaacgtgg acgatatccc gcttcaattt gatgttacag
     6001 ctaatgtcgc gaagatgaca gaaaacggag ataaatatga tacgagtcca aacaaaattt
     6061 tgaccaaggg taaattacaa ccttctatgt ctaaaaaggc cgttttttct gttgattttt
     6121 atgattcgaa taatattatc caagatcgta tgttcaattc gattttagct ccagctgcag
     6181 gtagatttta tcctaaatta actggcggaa acttgaatcc aggagcatat gcattttctg
     6241 caagtgcaga gataaatacg tggaatagtt acatctcacc aagtgattca acaggaaaat
     6301 ataatacatt tgctgatacc acagaatacg tggatggaaa taaccagaac cttccaggct
     6361 ctaccaatag tcggacattg acgatggggc tgactgaagg acgttttgat cagttgccat
     6421 ttagtcgctt tgatcgcgta gtcaactatt ttacaggaaa aaacgttaca caaggagcaa
     6481 tactgactca tactccagca agcacaagtg gattgaatca gacagccaat atcgtgtaca
     6541 gtgggaaaga tgcgcaaggc ggattgcttt ctcctgttgc tttaaaggta ttggagcagt
     6601 acaagctaga cggaacggtt ttaccaacaa acttaacagc cgatagtcag tggggaaagc
     6661 tcctttcttc tgcatcgtac caagtcacgg ccaattggtc agcaactgaa ctaaaaaagg
     6721 gttcgatcca ttacaggctt tattcgaaag gaacccaaaa attagcgaca ggatggacag
     6781 atcaactttt ccaaacgatc caatcaaata acgggggtgt aaatacagct acttctacac
     6841 tacctgcatt ggcttcaggg caatattatt ttgattatcg cttggtggac gatgtactta
     6901 ctaacatgta tccgtctttt gcgtacaaat ggcagtctga acaagctgga atcaccactc
     6961 ttcctcaaat aacagtggct aattttccat ctgtgagtgc agatagtcag ctaaaaaacc
     7021 ttagcagaca acctgagaca ggagatcccc tgtctgctct atcaggagat acgattcaag
     7081 aaaatgtgac attcacgtta acaaaatctg gggatgcgat aaccgacaaa aaaatcacga
     7141 tctcacttcc tgaaaatact acttatatca atggttcctt aaaattaaat ggtacaacta
     7201 tcgcagatac cggcattcaa caaggagttt ctgtcccagc agatctattg agcaaaatag
     7261 gagataccat tcatcttact tacaactatc aattaaatac tgtagatact tctttacaat
     7321 ccgtcagcat actgacaaag gcagctgtac tttccagcaa tattacatta gcagatggtg
     7381 ctaagcttcc taacccagtt gtccaaacat ctgctaaaac aattcttgtc cccaaacaag
     7441 aattaacatt agttaatgta ccagatgatt ttacatttgg taacgatctc cccaaaccgt
     7501 tgaagacaag ttattatgaa tcaaaaggcg atttttcttt tgatgtgcgg gatactcggc
     7561 ttccaagcac gtctccttgg caactgacag gtacattgac ttctttattt aagaatgatc
     7621 aaggtcaaga attaagcgga acaaaattat acttcaatca ttctggatca aagcaattga
     7681 ttcagcaagg tcaaaacacg ttgatttatg agagtgatgg aacagctaaa ggagaggttc
     7741 tcgttgactt tcccgatacc gatggtttac tcttagaggt aaatagcagt acgaatgctc
     7801 aacctggcgc aacgtatcaa ggcatggtca catgggaact gacagctggg ccaaccagct
     7861 aagtgattag aatttgtatg gatctgtgat aactgtcaca gatcctcttt ttcaaggatg
     7921 agaaggaaac ttctgctgtt aaaatcagaa atgcttgaag gagtggactt ggaacttttc
     7981 agtttgcttg aaaaaaatga acaacggcaa atcaaacttt tccaactttt attagaggaa
     8041 aaaggagagg tttccgttaa taacgtactt acccgcttat cagttgatcg ggctaccttg
     8101 aaagaagatc tgttttattt acaagctaac ttggaaaata tcaaacaatt ggaaattgtt
     8161 atccaagaag ggaatatttt attaatcaga catggaaata tagcagcagt cgatgtttat
     8221 tatcattgtt acttgcgcta ttctacaaaa tatcagatac ttctttatct gttggaaaat
     8281 ggctcatttg atcggcagaa acttgtcatg gaacttaata tcagttcggc tactttgtct
     8341 cgcagagtga aggaattaaa tgattcttta aatgagtttc atctgaaaat aaaaagcggg
     8401 cggctgaccg gtccagaaag tcaaattcgt tatttttatt tccaattatt ttggtttgga
     8461 cgtccctatc ttgaaaacaa aaaagaatac agtgatttat caggaaacac ttttttagat
     8521 ctgctgcaaa aagagtttag ttttgctatt acagaagaag gacgattgaa atttgaaata
     8581 tggctttcca ttatgaagaa gcgaatgcgc tgtacgaaaa aatccaaacc agattatagc
     8641 aatcttcctg ttggattgtt tcaaacgaca caatttttat tggacctcca tacatttttg
     8701 acccgttact tctttcaata tgcctttcct tggaatcaaa atgaaacgtt ggttttttat
     8761 cttttcatga tcagtaactt tacattagat acacataact cgcatgtcca ggagtttttg
     8821 ataagcgaaa aatacaaaga cgacgtagta gctcaattga atcgaacgtt taaacaatcg
     8881 atagaagaat atttctcttt ttatgttttt ccagaagaga tttcgcaaaa gattgcgttg
     8941 acagtgatgc aaaatcacgc tcattttgcc tattttagag gatggatgaa tatttttggc
     9001 caagaaggat taaaacagcg gttaactgaa gtagaagata aacaattcct ggttttatgt
     9061 gaaagattgg tcagacaaac atttgctatt gttgatttta ctgaagtaga cgaggaaaat
     9121 gcccgctttg aattatttgc tcgctatgca tctattttac ggacgctgat caagcagtta
     9181 gaattacgtc tgcaaattgc ttatgatctt ccttatgaaa ggctaatggc ggatatcgtg
     9241 aaagaaatca tccaggaaca attgctgaat acgatcaaat atgatttagt cgaatacgag
     9301 aaagaaaagc agtatgatat cattttgact agccaattga aagagtaccc gtcacagaag
     9361 tccgctaaag tttttgtatt cactagcaac gaatataatt atgatttccc ctatttgaac
     9421 caatttttaa aagaatgtta tttagaaaaa ttgaatttaa ggatgaataa aacatgaaaa
     9481 tcgtgatcat tggtgcttcc catgaaggca tcacagctgc cctaaatcta agaaaacttc
     9541 aaccgaaagc agaagtttta ctgatcgatg aagatcataa agatggacta ggctatgttt
     9601 caaatggaat caacctttat ttaaaaggaa agatacgtag tttagctgaa gttgcccata
     9661 acatgcggac tcttcaagaa agtggggcaa aactgatcac cgaatggaga gtaactgaac
     9721 ttgatccaga caaacatcag ctcatcctcg catccaaaga aggaaaaaac gagaccataa
     9781 cctatgataa attaatcgta gcgacaggtt cctctcctgt caccttgtat aaacaaattg
     9841 aagctgaaaa tgtctacacc tataaaaatc tagtgcaatc taagcaagta cttgccgctt
     9901 taaaagaagc aaaagaagta gtgatcttcg gtgctggtta cattggattg gaattagcag
     9961 atgctttgag aaataaagga tacattattc atctagttga ttatatgccg aatgttttat
    10021 caagatattt cgataaggat atgatcaaca gttttcaaaa tcagctgcaa actaagcaaa
    10081 taaattttta tccaaatgaa ttcttgatcg attggaaaaa atcagaagag aaagtagtat
    10141 ccgtacaact tctgtctcaa acaataaagg cagatatggt cattttttct gcccaaactc
    10201 gtcctaatac gacattgtta aaagaaaaag tggctttgta tgaggatgag actgtgatag
    10261 tcaatgaata tctgcagaca agtgatccgg acatctatgc gattggagat atcgtcccag
    10321 tcagttttga caaaaataaa cggcatctct ttttaccttt agttactcga gctgtacata
    10381 tggcaagagc agtagcgcta actttgagtg gtcagccaac cgcttacgac ctcagacaaa
    10441 agataacagc agcagtcatt atagactact ttttaggaac tgtagggctg acagaagatg
    10501 aagcgccatt cttggaacaa aataccggtt cgtgttcagg agaatttgat ctatttccac
    10561 aatacgacga agaaaacaaa acagtaaatg caaagctgat ctaccatccg gatacattag
    10621 aaataattgg cggacagctg ataagccaag aattcttatt atcagatttg aacctgcttg
    10681 cggatatcgt gaaacacaaa actacgattc cacagctagc agttgaggat tttggatttc
    10741 tgtctgaata cacctctcgt tttcactacc tcaatgagtt agcttttaaa gtactagcta
    10801 agagagcaaa tagcaataga gtagataaaa gactgtgaga aatatcacgg tcttttattg
    10861 tgtggggata taataaatat taatcaatcg agaataaaaa attcatataa tgaaatagcg
    10921 gaagaatttt ttgagcataa tattcgccaa aaatttatca agtgataaaa ttgttgtata
    10981 aaatgattat ttcaggagga ggagttaaat gtttttagca ttaaatgaaa ttatgcattc
    11041 aaagttacgt tatgcgttag ttgcaggggt gatgtttttg atcgcctacc tggtattttt
    11101 tttgactggg ttagcttatg gcttagctca agataatcga acagcagtcg acaagtggga
    11161 ggcagattcg attgtgcttt cgaaagatgc aaattcaaat ctagggatgt ccatgattac
    11221 taagaagata gcagaagaag tcgaaggtgg gaaagtggcg tatcttgccc aaacacctgg
    11281 agtcgtgact agtaaagaca gtacagaaga aggaaaaatc aatgttagct tcttcggtat
    11341 cgataaaaat caattcatca tgcccaatct tgttgaagga aaagcttttg ataacgatga
    11401 tgaggcagta ggcgatatta gtttgaaaga agaatatggt ctagccatag gagatacagt
    11461 taagttatct ggtagtgaca agacgtttaa actaactggc tttacggatc atgccaaatt
    11521 caatgtctca ccagttctct atacaaccat caatgcttac caagaaatcc gcttcgaaaa
    11581 agaagataca agtgaaaatg cacggattaa cgcaatagtt gtcagaggta aaatcagtaa
    11641 tttacctgaa gatttagaac agatcaaaat ttctaaattc atcaatgaat tacctggata
    11701 taatgcgcaa gtattgactt ttggatttat gatcggattt ttgatcgtta ttgcggctat
    11761 tgtgatcggg attttcattt atgtactgac gatgcaaaag attaatatct ttggtgtaat
    11821 gaaagcacaa gggatcacag gaggatttat tgcgcgttct gtagtggcac aaacattcat
    11881 tttatcattt gttggtattc ttcttggtct attaggaaca gtgggaacat cccttgttct
    11941 acctgatgcg gttccattcc aaagcaattg gctgtttttt ggcgtcatta gcttgttgat
    12001 gttagtagta gctgtgttag gcgcactatt ttctgttcgg acaatcgtaa aaatcgatcc
    12061 gttgaaggca attggataag aaggaggaag aacatgaaag caatagaatt taaagaagta
    12121 gaaaaaagtt tccaagatgg cgaccaagta gttaaagcat tgaaagaaac aaccatgagt
    12181 atcgataaag gggaatttgt tgcaatcatc ggtccttctg gttcaggtaa aagtactttt
    12241 ttgactttgg caggcggatt gcagacgcct agcagtggct ctgtatttat caatgatcaa
    12301 gaattcagta atcaaaaaga aagcgaacgc gttaaactac gttttaaaga aattggcttt
    12361 attttgcaag cttccaatct ggttcctttt ctgacagtaa aaaagcagtt attattagta
    12421 gataagatta aacatgaaca tcgtcaaaaa gaagcaaacg aattatttga gcagcttggc
    12481 gtggataagt tgatcaacaa gtatccagaa gaactatctg gtggagaacg gcaaagggtc
    12541 gcaatcgctc gtgcattgta caatgatccg tccattattc tagcagatga accaactgct
    12601 agtttggatt cagaaaaggc acgagaagta gtcgatatct tggcaaaaga atcaaaagac
    12661 aaaaataaag caacgatcat ggttactcac gatacacggc tgattgacaa atgcgataga
    12721 atatttatga tggaagatgg tgtattgaaa gaaaaagaat aaccaaaaag caggtggaaa
    12781 aatggtccat cgatacaaca aagatacaca gcaaaaaatt ttgaaagaag cgactgactt
    12841 gtttatgtcc aagggatatc tcggcacttc aacaagagaa atcgcacaaa aagttgggat
    12901 cacccagccc aatctctatc attatttcgg tgataaagaa aaactatata cggcagtttt
    12961 agaaaaccac ttaaaagatg ttggaaaagc tttgagagaa atcgttcaaa caagcgaaac
    13021 gggatttcaa gtaaccttga caaaaatggc gcagtttttg attgagacac atctggtcga
    13081 tttgtttctg atgcttcacg atcttgaaag caatctttca aaggaaacaa gagatcatct
    13141 tttctttcta tggaagaaaa attatcgtga accttttgaa gaaatctttt cagcaaacca
    13201 atctgtgcta agaaatggga tttcaaaaga aatcgcagca aggcattttt tcttagtgtt
    13261 agcaccttac atcactcggt cgaaaagacc tgtggaagaa ccgctaaatg tggcgcaatt
    13321 gatcgattta tatttgaatg gtgtattagg agaagcgtaa agctgttata tagggagaaa
    13381 atagttaggt gaagaagcaa gatatgacga agatcgttat atcttgcttc tttatatagg
    13441 tagtttttca taaatacaaa tttgttattc tctcatgatt atcagggtat atttttattt
    13501 attatgtcgg tgttccagga agtgttttaa tttaataacg aagaaattaa ttgtttgtag
    13561 aatgaaggaa actatttatt gggtagatga atcaccacga atagtcaaaa ggagatttca
    13621 ggatatgcca aaaatgaaaa aaatcatttt agcagtaatg ttattttcta cggttatatc
    13681 ttttacagcc ccttttttgc ttacttaagc tgattttaag ttttggccct tagtctggat
    13741 ctacgaggtg agagtcaaga atgaaaaaaa taattggagc ttttgtattt gcaatcatta
    13801 tcattattat ctgttttcaa atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg
    13861 tcagcagtac gaacagtacg gttacaaaga caaaggaatc atttgatgat acagaacaag
    13921 agacattaat caaaatcgga aaaaatgacc gtacactata tacagatgac tcgctaacta
    13981 ccccacttgc aactataaac ggcggagagc tggcggagtt tctttctgaa acaaaaaatg
    14041 cttatcagat caagacaaat gatggctata ctggatattt agccctcgaa gatggaacca
    14101 aagtcacaaa gaatatacaa acaaaaccgg aagagctatc tgatgcagtg attgtattag
    14161 atcctgggca tggcggaaat gatactgggg cattaagcaa tgatgaacaa acagaagaaa
    14221 aagatataac attaagtaca gccataaaag taaaaaaggc gctagaggat gctggagcaa
    14281 cggtttattt gacccatact acagatgaat tagtacagct tggagatata tgcgactaca
    14341 gtgaagaaaa aaaggcagat gtctttatca gtttacacgc agactctacg gaatatgcga
    14401 atgaagcaac tggaatcacg acatattatt actatggaca agaggaaaca ttagcacaga
    14461 caattgctga ctccttcact gatctgcctc tggattcaag aggaatctcc actgggaact
    14521 atcaagttct tcgagaaaat cttcaacctt ctattttaat cgagatgggt tatatgaata
    14581 atgatagtga tttaaaggaa ttagtaacag acagttatca gcagcagatt gctgcaagtt
    14641 tgacacaagc attgaccact tattttcagt aataaataag cccagccaaa caaaaatagc
    14701 tcctcatatt tttcgtttgg ccgggcttat tatcgcagat gcaagctttt aaacaccgtt
    14761 taaggaagag gtcgtgagcc tgccgttcaa ctctgagtca caaggagcaa tttcaaaaaa
    14821 cagcttctca tgtttgatga ctattgtgac ttgtgaccga ggagttggca tgagaacacc
    14881 gtttaagaaa aaggttgtga aatcaacgtt ttgttctgag tattagagaa acaaacggaa
    14941 aaatagttcc tcatattttg ccgtttgttg agctaatacc gaggatcaga tgattgaaca
    15001 ccgtttattc ggaacaaggg actgtgacat gacttttgtc acagttccat tattttttta
    15061 gtagcttcaa agctttttct gtattggcaa aatgaagttt ggcatatttt tcaagcatag
    15121 gcatgccgcc tttctccaat agttttgccg ctacttggtc agatttcgtt ccagctcctg
    15181 acggaagggt gattccagct tcagctgatg ctttgtctaa ctcttccaaa aagctggcac
    15241 gactgatgat agaagcagca gcaacagcta aatgatattg ttctccttta gtaacgaagt
    15301 acagtttttc ttgtaattga tttttttcta aacgcgcata ttttttaaaa tttgcttccg
    15361 gcgtaaactg atcgatcaaa atcgcttctg gtttttctgg ctcgattttt tttagcagca
    15421 aatggatcgc ttgattgtgc aaagcgactt tcatccgaac ggcattgtat tttggttgga
    15481 tctcattgta tttttctgga gataagacaa gcagtttata ggggatggtt ttttttaaga
    15541 tgtcagcaag ctggatgatt tggctatctt ttaactcttt tgaatctcta acaccaagtt
    15601 tacgtaattc tgcaagctga tcacgtttca cataagcagc acatactgtt agcggaccga
    15661 aatagctgcc atttccgacc tcatcactac ctaaaacgga taattgactg atattttttg
    15721 gcaaagaaga ggctgttttt gctggcttgg cttgtttatc ctgtgcctcc ccccatttat
    15781 cagcttctat tgctgcattc gtaccttgaa acatcacttt tccggatgta taagcggtaa
    15841 tggtcgtccc gtcttttttt gcagcaaagc tagtataagg aatctgttta tttaacaaat
    15901 aagagcgata gtatgctttc atttgactaa gttggtcgtt tgttgctttg ataatgacgt
    15961 tgctcataaa attctcctcg tttttataca taaaactata ccgcaactca cgacttttcg
    16021 ttttctaaga gattgcgaga catagcattt ttttaaagtt tatcatataa tagttaagta
    16081 tgtgaaaaat atttgtggag aacggtgaaa gaaccaactt catggtacac taaataaaac
    16141 tttcatcaaa taggaggaaa tgattcgtat ggcgcacgaa aaaacacgct acaaagcggt
    16201 gattgccaac caaacatata ctattatcgg acgggaaacc aagcatcata tggatatcgt
    16261 aacgaagctg atcaatgaac agttagcaga attgaaacaa ttatcaccac aaatggataa
    16321 tgaacaagca gcaatcttga tggctgtcaa tgcgctttcg gatcaattga agaaacaaga
    16381 aagaatattg gagctggaag aagaaacagc agaattaaag aaaaaaatga tcaaatttac
    16441 agagcttgaa aatcgtgtga aacgcattga agcaatcgag aacgaagctc gggaagtatt
    16501 gaaagaaaat ggtcaagcag atcataccat taaaaatcat gtggaagcac aacagatttt
    16561 aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct aattaagatc cttttatcaa
    16621 aataagaaaa atttaaaaaa gttagaagag aaaggaacgt agcatgctga gtttgctgat
    16681 tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg ctttgcaaat
    16741 catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt acaaaccatt
    16801 agcaagtcat ttggaattat acattccata cccagctgtc accccaacat caaagttagc
    16861 attttttgat caggtatttg cctttcacct agatgaagct ttttatgcag gaactgcttt
    16921 tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt ttgtccatgg
    16981 attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca tcctgtcact
    17041 aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg ttccattaga
    17101 tgtggtccaa aatcaattcg gcgagcatag tttcgctcgt tttatcgttg agagaacgcc
    17161 atttcttacc aacaagatgt atgagctttg ggtaacgaat attttgaatt aaggaaaata
    17221 gtgctggaaa atatttcgtt ttttgtacag agtagtttat ttaagaaggc tgcgtagcgt
    17281 atgtccggca cagctctttt ttcatagtga aagataaggt gataaaatga ataaccgaat
    17341 ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc agacaatttg tagtgactgc
    17401 acaaggaaaa gaagaactgg cagaattggt accagtgagt gagaaacaaa cgatcacaaa
    17461 ctggcttcaa gaaactgagg atggattgaa agtgcaacgt ctgcgaggcg ggatccctat
    17521 acctaaaatt gaaaatatcc gtccccatat gaaacggatt gagatcggag cagacttgaa
    17581 tggagtggag cttgcacaag tttccagagt attgtctacg acaagcgaat tgaatcgttt
    17641 cattgatgat ctttctgaca gtgaaatcga atttgcacgt ttgtatatgt gggcagatca
    17701 gttagtcacg atccctgttt tgagccgccg tttaaaggaa gcaatcgatg aagacggacg
    17761 cgtgactgat gacgcttctc ctgagttaaa gagcataaga cagaacattc ggcgaagtga
    17821 acaagctgtt cgtgaacagt tagatgggat cgtccgtggg aaaaatgcaa agtatttaag
    17881 tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc ccagtcaaac aagaataccg
    17941 cggtgtattt ggcggtgtag tccacgatca aagtgcttca ggtcagactt tatttattga
    18001 accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa taccaaatcg ctgaacgaaa
    18061 tgagattcaa cgtattttaa gtgaactttc tgctgaactt gttccccatc gtcaagaaat
    18121 catccacaat acttatgtga ttggtaaaat ggatctgatg aatgcaaaag cacgatttgg
    18181 taaagaagta aaagccatcg tgccgggaat cagtgaagac aatcatgtcg tcttgaaaca
    18241 agcaagacat ccactgatcg atcaggaaaa agtagtttct aatgacatca caatcggaaa
    18301 agactatcaa gccatcgtca ttacaggacc taacacaggt gggaaaacga tcacgttgaa
    18361 gactttaggt ctgttacaac tgatggggca agcaggatta ccgattcctg caggtgaaga
    18421 aagtcaaatc ggtatttttg aggaagtatt tgcggatata ggagacgaac agtcgattga
    18481 gcaaagtcta tcgacctttt cttctcatat gacgaacact gtagatattt tatcaaaagt
    18541 caatgaaaaa agtctggtat tatttgacga gctcggtgca ggaacagatc cgcaagaagg
    18601 tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa aagtctgctt atgtgatggc
    18661 aacaacgcat tatcctgaat tgaaagttta tggatataat cgggcaaata cgattaatgc
    18721 cagcatggag tttgatgtgg atacattaag tccaacgtat cgtttgctga ttggcgttcc
    18781 tggacgaagc aatgcgtttg aaatctctag ccgattaggt cttgatacag aagttattga
    18841 tgaagcgaag cagttgatga atgatgagag ccaagattta aacgaaatga tcacagattt
    18901 agagaatcgc cggaaaatgg cagagaccga atatctggaa atgcgtcatt ttgtttctga
    18961 ggcacaagaa cttcacgatg atttgaaaga agcttacagc tatttctttg aagaacgtga
    19021 aaaagaaatg gaaaaagcaa agaaaaaagc aaatgaagtc gtgtcagagg cggaagaaaa
    19081 agcagaaaaa atcattgctg atattcgtaa aatgcaacag cagattgggc aaggaaatgt
    19141 gaaagagcat cagttgatcg atgcaaagac ccaattagca aatctacatc aagaagaaac
    19201 tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa aaaacgttga aaccgggaga
    19261 cgaagtgttg gttacgactt acggccaaag agggacgctt ttacgtaaaa acgggaatca
    19321 gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc gaagacgagc tgacgcctgt
    19381 tgctcctcaa aaagagccga cacaacgagt tatccatgct gtacgatctg aatctagtag
    19441 tcacgtgccg aatcaattgg accttcgagg aaaacgatac gaagaagcgc taagtgaggt
    19501 agatcaatat ttggattctg ccattctagc tggttatcct caagtgacta ttgtccacgg
    19561 aaaaggaaca ggcgcacttc gcaagggaat tactgattat cttaaaaacc atcggagcgt
    19621 caaaagcttt gagtttgcac cggcaaacca aggcggaaat ggtgcgacaa ttgttaaatt
    19681 taagtaaagc ttactaaagg taagcagatg gttcgctatc caagtataga aatgctatac
    19741 tagtcataga ttttaaaaat ggaggtcttt gatatgtcac aagtaattac agataagaca
    19801 tttaatgaag aaacagataa aggacttgta ttgattgatt tttgggcaac ttggtgtggt
    19861 ccatgtcgta tgcaagcacc gattttggat cagttggaac aagaatatga tgaagaagaa
    19921 ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa ctcctcaaca attcggtatc
    19981 atgagcattc caacattaat gttgaaaaaa gatggtcaag tagtagaaaa agctgttggt
    20041 gttcattcaa aagaacaact acgtcaaatg atcgatcaat atttataaaa acttacagca
    20101 aaaataagct gagggtggga ctgaatccag ttccgtcctc ggtttttttg cggttgagtt
    20161 aaggttattc aaattaatat tttttgagta taattacgtt gtcaggctgg attgtttttt
    20221 tgacagacat gctataataa ctttaattga atatgaaggg gagtaccaga tgaacgaacg
    20281 aatcaaaaac aaattagctt tacttcctga tcagcccggc tgttacttga tgaaagataa
    20341 aaatggaacg atcatttatg tcggcaaagc caaaatatta aaaaatcgtg tccgctcata
    20401 ttttactggt agccataata cgaaaacgga acggcttgtt agtgaaatcg aagactttga
    20461 atacatcgtt acagaatcga atatcgaagc attgctctta gagatcaatc tgataaagaa
    20521 aaatgatcct aaatacaaca ttatgttaaa ggatgataaa acctatcctt tccttaagat
    20581 aacgaatgaa aaatatcccc gtttggtgat tactcgtaag gtactaaaag acaaagcact
    20641 gtattttgga ccatatcccg atgtcggtgc ggcgaatgag acaaagaaga tcctcgatcg
    20701 attatttcct ctgagaaaat gcaaacctag tcagacgaag gagccttgtc tttactatca
    20761 tttgggacaa tgcctgtgtc cttattattt cgacgtagat ccgaaagtct atacgaaaat
    20821 cgttgatgaa gtgaaaaact ttttaaacgg aggacacgaa aaaatcgagt cagagatcca
    20881 tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa aaagctgcag aataccgaga
    20941 ccagatccgt gccattgaga caatcatgac tcgccaaaaa atgacaaata ctgatttact
    21001 ggatcgagat gtatttggtt atgcgattga taaaggatgg atgtgtgtcc aagtgttttt
    21061 cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg tttcctttct ataacgaagc
    21121 agaagaagat ttcttgactt acctcggtca gttttatcag gaaaatgagc atttcattcc
    21181 taaagaagtc ttgattcctg atacgatcga caagcccagt gtagaagctt tgttagctac
    21241 gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta gttgaattgg cgagtaagaa
    21301 tgcctcagtt gctttgaatg aacggtttga tctgattgcc agaaaacagg aacggacgat
    21361 cggagcagtt gaacgtttag gaaacgcgat gaatatcccg accccaatac gtatcgagtc
    21421 ttttgataac tctaatatca tggggacgaa tcctgtggct gcgatggtcg tattcattga
    21481 tggaaaaccg gcaagaaaag aatatcggaa atataaaata aaaacagtca acggaccgga
    21541 tgattatgca tcaatgagag aagtcatcta tcgcagatat tcgcgagtga ttcgcgaagg
    21601 gttgccttta cctgatttga tcttgatcga tggaggaaaa ggacaagtag atgtggcaaa
    21661 agatgtctta gaaaatcaat taggaatcga tattcctatt gctggcatgg taaaaaacga
    21721 taaacataaa acaaatgagt tgctattcgg tacagatttg gctgtagtac cgcttgagcg
    21781 aaattcacca gagtttttct tactccagcg aatccaagat gaagtccatc gattcgcaat
    21841 cactttccat cggcagctac gcagtaaaaa cagttttgct tcccgattag atgaaattga
    21901 agggttagga ccaaaacgaa aaaaaatgct gcttaaagag ttcaaatcgt tgaaaaatat
    21961 cacagcagcc acgacagaag aattacaaga aatcggactg ccgaaaaatg tcgcacaaaa
    22021 cgtatttgat aaattacatc aggaatgagg aaagggcact ttacaaagaa agtaaagtgt
    22081 cctatcattt tatgtacaac aaaaagagcg tttacgtttt gttggtaaat aataaatcgc
    22141 ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga aggagtgaag aaatgttaga
    22201 agtcaaggat cttgttaaaa cttttgggaa ctacacagct gtcgatcatg tctctttcca
    22261 aatccccgat ggaaaaatca tggggctaat tggccaaaac ggggctggga agacaacgac
    22321 ttttcgtttg atcttggatt ttttgacaca agaccaagga gaagtgttat ggaatggcca
    22381 tcatcttggg gagaaagatt atgatgtcat cggttatctt ccggaggaac ggggacttta
    22441 tccgaaagta acgattcaag accaactgct ttattttgca gaacttagag ggaaatcaag
    22501 aaaagagatt gaaccaaaaa tcgacttttg gatggaaaaa tttcaggtaa aagggaaaaa
    22561 gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa aaagtccagc tgatcgccac
    22621 gttaatccat gaacctaaat tgatcatttt ggatgagcca ttcagcgggt tagatccggt
    22681 aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa aaacatggtt catgtgtcat
    22741 tttttctagc cacaatatgg ataatgtaga aaaaatctgc gatcatttga tcatgttgcg
    22801 aaacggaaaa atggttttga atggaaaagt acatgagatt cgtgaatcat ttggccgtac
    22861 gaagttattc ttggaatctg gtttaagcca acaggaagta gaagaaatcg aaggcgtgaa
    22921 aaaagtgatc cttcatgaag acaatacgtt agagattacc ttagaagatc ctgaagctgg
    22981 gaaagaaatc tttactcgtg cgactcagtt cggctacatt cctatgttta accagcaacc
    23041 gccaactttg gaagagattt tcaaattgaa agcaggtgaa atcgatgcgt aagttttgga
    23101 ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat ttctttcttg atcatgatat
    23161 tagtcccttt tatcatgctg ggtatcattt atgtagcagg aaactttgct caacaaaata
    23221 gtgatgtcaa taaaatcggc gtattggctg ataatgagca aatcagtcag caaatgagcc
    23281 aagtcaaaac agatgatttt gagttcaaag cagttgattc tgaagaaaaa gcacaaaaag
    23341 agctttcgga agaaaaaatc gatgcgtatt tagtagtctc aactgagaat gataagataa
    23401 atgggatgct ttatagcgaa aattcattag gacaaagtac acaactactg atacaacaac
    23461 aactgactgg cttgcaatcg atgcttcgag cgagtcaatt aggcatttca ccagaagaag
    23521 tagctagctt gagccaacaa gcaagtttga ctcgacaaaa agtcaatttt gattcaaaag
    23581 gaaaaatgac agtcggagaa gataactcga cagtccaata cgtcgtaagt tatgtagcaa
    23641 cgattgtctt gttcattatt attttgacgt atgcgcagat cattgcacaa gagatcgctt
    23701 ctgaaaaagg cacgcggatc atggaagtga tcctgtccag tacgactgca caaaagcatt
    23761 tttacggaaa attaacaggt gttttacttg ttgcattaac gcaaatggca ttatacggta
    23821 ttatttttgg tataggatac agtcaattca aagatatgga aatggtcaaa aatatattag
    23881 acggtatctc attggataac ttgattggac cattcttgtg gtattctctt ctattcatga
    23941 tctttggcat tttgattttt tctgttttag cagctttatg tggctctttg gtcaacaaag
    24001 cagaagatac agcaaaagca attttgccag tgacttatct atctctaggc ggatacatgc
    24061 ttggattgat actaggagct tcagatccaa acaatatcgt tattcggatc acttcgtata
    24121 ttccattctt atcttcatat attatgccga ttcgcttagc aaatgagaca gtcggtatgg
    24181 gtggagcatt gatttccttg ttggtacttg tagccgtaac attcttattg atgatcttgt
    24241 ctgcaaacat gtacaaatcg aatgtactcg tttatagcga aggcggatta tggagttcat
    24301 tgaaacaatc gatttcaatt atgaggaatg aacgaaaaaa agcataaaac aatcctaaaa
    24361 tacagaagga agctggacat ctattgttcc cagcttcttt ttgtatagac ctattttatt
    24421 tattttacta tattggatat atattcattg aaaatagtgt ataacaactt tataatattg
    24481 agtataccaa tttttggagg cgttttaatg gaccgttatt tagatttact ttcagaaaaa
    24541 ttcccctcaa cggaggatgt catcacggaa atcatcaatc tcgaagcaat tttgcagctt
    24601 ccaaaaggaa cagagttatt tttgagtgat atacatggcg aatttcctgc ttttgatcat
    24661 attctgcgta tcggttcagg aaatcttaaa gaaaagatca aagacctttt ttctgaacaa
    24721 atgacggaaa cggatatcag ccaattcact atttttacgg cttatccaga atatgcatta
    24781 acgactgatt ggtacaaaga acaagataaa tcgcagctga tccatcattt gatcgatctt
    24841 ttacggttta cgacagtcaa atatacccgg tcaaaagttc gaaaaggatt gccgaaagaa
    24901 tatagctata tcattgagga actcttgtat atcgatgacc gtatcaatgg aaaaaaagac
    24961 tatgtaaaaa aaatcattga acaacttgtt ttgatgaagg aagaagaaaa atttttaacg
    25021 aaacttgcac agacgatcca aaaatctgtc atcgatcatt tgcatatcgt tggagatatt
    25081 tttgaccgag gaactcaagc agccaaagtg atggatcgaa tcatggattt ttcttctgta
    25141 gatatccaat ggggaaatca tgatattttg tggattggtg cgtattgcgg ctctgaggca
    25201 tgtttactga cacttttacg gattgcagca cgctataatt atttatttga attagagaaa
    25261 gaatatgggt tgaatttacg tccattgttt tcttttgctc atcaaactta tcagaagaat
    25321 ccagtatttg cgcctaaaaa cagtaaaaat ctatccgaaa gagagcaaga agaattagaa
    25381 aaaatccatc aggcactttc tatccttcaa ttcaaatcag aacatcagtt attggatcgt
    25441 cgacctgaat ttaaaatgga cgatcgtaaa ttgctggaaa aaatcgacta tgaagcatca
    25501 acgattgatt tagaaggtca gctctatgag ttgaaaggaa cttgttttca aacgatccag
    25561 ccagatgatc caaaaaaatt cttggaagag gaacaaaaag tgattgatag tttgatgtat
    25621 tcattccagc actctttgag aatgcaaaaa catatgacgt ttttgatcga aaaaggggga
    25681 atgtatctca cgaacaatca gcatgtgctg tttcatggct gtatacctgt agacgagaag
    25741 ggacagctct tagcatttga attagaccaa tcttatcgcg gaaaagaact gctgggcttt
    25801 tttgaaaaac agataactga aagcgcaaaa gatctgactg caaaagaaga tttagcaacc
    25861 gatttgattt ggtatgcgtg gagtgggcca ttttcgcctt tattcggcaa gagtaaaatg
    25921 gcgacttttg aacgttattt tctcacagag cctcatacgc acgtcgagaa aagcaatcct
    25981 tattatcact tgcgagatga agagtggttc agtgaaaaaa ttttagcaga attcgacaca
    26041 aaagaagaag ggtcagcaat cattaatggg cacacgccag taaaagtaaa aaaaggggag
    26101 tcaccgatca aagcaaatgg aaaagtattt gtcattgacg gaggtttatc caaagcttat
    26161 caaaaaacga caggaattgc cggttattca ctattatgca attcttatgg tttccaaatc
    26221 gtgacacatt atccgttttt gcctattgaa caacaatttg caaaaaaatc cgaccaaaca
    26281 aatgtgaaaa aagtaatcga acagcaacta ccacgcaaac tcaatcgtga tacaacaaaa
    26341 ggtatggaac tgcagcagca gattacccag ctcaaaaggc tgttggacta tcgaaaggac
    26401 gattgagaag aattccagtg aacattgttc catagactgg agactgagac aaaagtcgtg
    26461 tcgcagtctc tttttccgga taaacggtgt tcttctgcca actcctcggt cacaagtcac
    26521 actattcaac aaacatgaaa agctgttttt tgaaattgct ccttgtgact cagagttgaa
    26581 cggcaggctc acgacctctt ggtataaacg gtgttcaaaa gccagtttct cggtattaag
    26641 ccaaatttat gcaaaaatgt gaaaagccat tttcacaaaa tttttcttaa tactcgaaac
    26701 taaacagctt tttcacaacc ccctcagaaa gtctattaaa attcattcac aaagaaatcg
    26761 aataagcgct gttcagatgg atgtacacgg tgggtaagtt ccggatgcca ttgaacaccg
    26821 aggattttat gcatatcatc tttgctttga accgcttcaa cgagtccatc gttagaaaaa
    26881 gcgatggctt ttaataaagg tgaaagatct ttgactgcct gatgatggta agaattcaca
    26941 aaatatggat cggggagaag gttgcttaac aaactgtctg aaacaatctt gacttcgtga
    27001 gtcgtaaatt gcggggcagt gggctgttgt tcatgtttta ctgaccactt tggatataat
    27061 gaaagatctt gataaagtgt accttccaaa acgacattca atagttggag tccccggcag
    27121 actgcaaaaa ttggttttct ttgtttcaat gcttccaaga tcagtgcctg ttcaaattgg
    27181 tctcgttgaa tatttgtttc cgttagcttt ggatgaggat cttccatgta aagttgtgga
    27241 ctgacatctt gaccacctgc aagaataagt ttgtcgattt gtccaatcag ttgaggagct
    27301 gaatctggag acatgactgg cagaataaga ggcaatcctt gagcatctaa aactgcatcc
    27361 acgaatcctt gcggtgtata gctcacttga tttccttgga aagtatctgt tgctcgaatc
    27421 agttgatttc cggcaatgcc gatgattggt ttcatttgta ttccccctaa agtagactta
    27481 ttgtaacaag gatttaaaaa aaattaaact atgtaagact tgcccctata cttatctgtt
    27541 cttgctgtgt tactatctgt aaagagaaat ttcgtgtgaa attcatgacg atagttgaaa
    27601 atattgctta atttgttata ataaaaagta agcagaaata aaaaggttgg gcgtaagctt
    27661 caaggatcta gttcttcaag gggtgagaag gacattttat tgaaggggcc tgcctatact
    27721 aagttatctt tagaggtaca tactttcaat gaaaaaagaa agcatgtacc tcttttttaa
    27781 atcgaaagga agtgaagcaa gtgttagaag ctttggaaca aatccgaaaa gcagaagaag
    27841 aaaatgaaaa gcgtgctcaa acactgcggc agcagctgaa gatctatgca acagaaaaag
    27901 agcaggaatt ggaaacgatc caaacaaact atcgacaaca actaaaacag ttggtggaag
    27961 aaaaagaaca atttgaaata caagaattag aaaaagaaaa aatgaaattg aaagcagaag
    28021 cagacaaggt gcaagcaact ttgaaagatc aatatcaaaa gcatcagagt caagcgatcg
    28081 gtgcgatcat tgaaagggtg aaagaaactt atggccgtca ctaagatgga aaaagtgacc
    28141 ctcatttcag ataaaagaaa tcaggagagg attttacaag cagtacaagg aatccaaaac
    28201 gttgaaatcc gggatctgta tcaaaataga acaaacaacc agtgggtgga gaagtatttt
    28261 ccagatacca cgattattga taaagaagca aaattaagcg ctttaggtaa tcggttgaaa
    28321 gacatccaag aagctatcca attcatacaa caccacggcg atagccgtca aaagaaactc
    28381 catttgaaac gtcaagaact ttccttacaa gatttggaag ccaattattc cgaagaaaat
    28441 ttcataaata aattgaaaga agtccttgat ttaaaagaaa agtgggaaca actaactgaa
    28501 gaacgtgatc gtttagaaga agaggaagaa tggcttcttc attggcaaca gctggatatt
    28561 gctccaagta tgtatgcaag taagacgacc atttttgaga tggggactgt gagtgcagca
    28621 aattttgaaa gtttcaagga agaactgagt aagcttgatg acgtttatat ggaagaaata
    28681 gattatacgc caaaacaagt ttatctttcg tatgttgctt taaaaaaatc agttgctgtt
    28741 atagaagaaa ttgctgggaa atacgggttc aataaagaaa cgtatcctta tgaacaaaat
    28801 cctaaagaaa aactgcaaga gaccaagcag caattatcca ttgttcacga gcagcaaaag
    28861 aagataacga cagcgttagg tcagtgcagt ggctatatcc aagaatttga atgggtagaa
    28921 gaagtcacac ttgccgttgc agaacgagaa aaaataaaag aacggtttat ccatgcgact
    28981 tatctgatcg ttttgcaagg ttgggtagat gcagaagaaa aacagaattt gctgcatgtg
    29041 ttaaacgaaa ccgtatcaga agaagatata tacgtcagtt tcgaaacacc aaactcagaa
    29101 gagattcagc aggaagtccc tactaaattg aaaaaccatc cgcttgtcga accttttgaa
    29161 ttgctgactg aaatgtacag tctgccaaaa tatgaagaag tagatccaac accttggatg
    29221 acaccatttt atcttgtctt cttcgggatg atggtcgcag atgttggtta tggtttgctg
    29281 atgctcattg ggacaatact agctcaaaaa ctacttgttc taccacgcgg gatgaaacgt
    29341 ttcgtaaaat tctttgagat tctctcgata ccatcgatca tctggggatt gatctacagt
    29401 tcatttttcg gacagtcatt accaaaagaa ctgtttggca tccgcttgcc tttcccaatc
    29461 ctatctacga cggatgacgt caatacgatt ttgattttat ccgtgatttt cggtctgata
    29521 cagattttgg ttggattatt tgtggcagca aaggaaaata tcaggagaaa agattacttg
    29581 agtgctgtca gtgatggatt tgcctggcaa gggatcctaa ttgggatcgt tattgcgttg
    29641 gttggtgcca tgttgataaa aaatacagca tttgtttacc ttggcgctgg gctatcgatc
    29701 attgctgcct tatgtatctt gatcgttccg attatccaaa cgccgtctaa agtcaaaggt
    29761 gctgccaaag gtgcgtataa tctttacgga ctgaccggct atatcggtga tttagtcagt
    29821 tatacacgtt tgatggcatt gggtatttca ggcggaagta ttggagcggc attcaatatg
    29881 ctagttgctt tcatgccgcc agctgctcga ttcagtgtag ggatcctttt gatcatcgct
    29941 ttacacgcac tgaatatgtt tttgacactg ctaagtgcct atgtccatgg cgcacgattg
    30001 cagtatgtcg aattttttgg gaagttttat acaggaggcg gacgtgcctt tgatccgctg
    30061 aaaacggcgg aaaaatacgt caacatcaat cacaagaaaa aaaacaataa ataattggag
    30121 gatttttata taatgatgga ttacttaatt agtcaaaatg gtggaatggt atttgcggta
    30181 ttagcgatgg caacagcgac gattttttca gggatcggtt cagctaaagg tgttggaatg
    30241 acaggtgagg cagcagcagc attgacaaca agtcaacccg aaaaattcgg gcaggctctg
    30301 attctgcaat tgcttccagg cacgcaaggt ttgtatggat tcgttattgc ctttttgatt
    30361 tttatcaact taggaaatga catgtcagtt gtacaaggac tgaactttct aggtgcttct
    30421 ctgccgattg cttttacagg cttgttttca ggtattgccc aaggaaaagt cgcagcggca
    30481 ggtattcaaa ttttagcgaa aaaacctgaa catgctacaa aaggaatcat ttttgctgcg
    30541 atggttgaaa catacgcgat tttaggtttc gttatttcct tcctattagt gttgaatgcc
    30601 taagcataaa ggaggaaaag cagatgaacg ccattgaaaa aatcatctct cagatgaacg
    30661 aagcagcaga gcaggaaaga gcggcgttag aacaagaaga gcgaatgaag atcgatcaaa
    30721 actttgaaca aaaacgtacg caagttgaaa cagaacatca aaaacaaaaa gaaaaacaaa
    30781 tcgaattact cgaaaaaaaa taccgtcagc taaggaatcg ccagcaagta gaagtacgac
    30841 aagaaaattt gaatgcgaag caggaatttt taagacgatt gtttgcagat gcggtaactg
    30901 aaatggaaaa ttgggatgaa tctgaacaaa tccagtttat caaaaatgca ttgtattcat
    30961 tacctttgac aggaaaagta gcattcattg ctggagaaaa atcagcagcc tatctttctc
    31021 agacgttgtt ggatgaatgg aacaatgaac ttccctttat gatggtttta agtgatgaaa
    31081 ctgttgctga tcaagcgggt tttctgataa acgatcaagg agttcagtat aattttcttt
    31141 tcagcagcct tgtacaagat atccaaggaa cgatgagttt tgagattgcc aatcaattat
    31201 tcgaataagg agggatcagt gtatggaata ccacgaacta aatcccttga ttcgcggaag
    31261 agagcttgaa cttttgtcca atgagacatt tgaacggatg attcaaacaa attctgtcga
    31321 agcacttggt gaaatattga aatccacgat ttacagtcca tatatttatg acggatatga
    31381 atatgacttc gaacagaatc ttttaaagga gcagagccgt ctatttcgct ggttaaagga
    31441 atcagcaccg gagcctgaaa tcgtttggat ctatacgatg cgttatactt tccataatct
    31501 gaaggttttg acaaaagctg agatgacaaa tcaaaatctg gaccatttgt atatcgacga
    31561 tggattttat tcattagaaa ccttgaagga tgccattcat acgcaagttt caaatgaact
    31621 tcctgaaagc attatggaca gtatacgaga agtacgtgat tattttgaag agtctaccat
    31681 tcttcaggga attgacgtga tttatgatcg acagtttttg acagagcagc gacggcttgc
    31741 cgataagcta ggatatcaag aattgctgac agaagtcatt gcttttattg acttgaccaa
    31801 tatcacaacg atggctcgag gaatattgca gaaaagaaca tatgggttca tgacgacggt
    31861 actttccagc tcgggaagta tcgaaaaaga agtatttttg aactttgtag ataaagatat
    31921 ggaagattac acgaattttc tattagagac agattatgct gattttctgc gccctgtcat
    31981 ccgtgaggga aatattgatc tcgtaaagct tgaacagttg aaagatgatt atctaagttc
    32041 attgtatcaa gaagcacaaa cccaggcttt cggaccactg ccactactag cctttttgaa
    32101 cgcaaaagag gtagaaagca aaaaccttcg tttactggtt atcggcaaaa gaagccattt
    32161 cgaatcagaa acaatcagaa agcgggtgag acaagtctat gacgcataag atcggtgtag
    32221 ttggcgacaa agattctgtt ttgccattca aattgtttgg ctttgatgtt cgttatggta
    32281 caaccaagca aaccgttcgt caggcaattg aagaaatggc aaagaatgag tatggcgtga
    32341 tttatgtcac agagcaatgt gcagcattga gcccagaaac gatcgaacgt tacaaggaac
    32401 aaatgatacc tgctatcgta ttgattccca atcatcaagg atcattaggg atagggctgg
    32461 aagaaattca aaaaaacgta gaaaaagcag taggtcaaaa tatcttatag tatgaaaagg
    32521 agatagaaat tttgcaaatt ggaaaaatca taaaagtctc cggacctctt gtaatggctg
    32581 agaacatgtc tgatgccagt atccaagaca tgtgtctagt gggagatcta ggagttatcg
    32641 gagaaattat cgagatgcgt ggcgatgtgg cgtctattca agtatatgaa gagacttctg
    32701 gaattggtcc aggcgaaccg gttcgctcta ctggtgaagc tttatctgtt gaattaggac
    32761 cagggatcat ttcacaaatg tttgacggaa tccaacggcc attagatacc tttatggaaa
    32821 tcacacaaag taatttctta gggcgcggag ttcaattgcc cgcattggac catgagaaaa
    32881 aatggtggtt cgaaccaaca gtagaagccg gagaaacggt gagtgctgga gatgttatcg
    32941 ggatcgtaga agaaacaaaa gttataaaac ataaaatcat ggtgccaaat gggataaaag
    33001 gaacgatcaa aacaatcgaa aaaggttcat tcactattga tgaaacagta tgtgtcattg
    33061 aaaccgaaaa tggtgacaaa aatttgacga tgcttcaaaa atggccagta agacgtagtc
    33121 ggccaatcaa agaaaaatta aacccggatg cacccatgat cactggacag cgagtgattg
    33181 atacattctt cccagtgaca aaaggaggag cagcagctgt acctggtcct tttggtgcag
    33241 ggaaaacagt cgttcagcat caaattgcaa aatgggccga tgtggacatg gttgtctatg
    33301 tgggatgtgg cgagcgtgga aatgagatga cggatgtttt aaatgaattc cctgaattga
    33361 ttgaccctaa tacaggagaa tcgttgatgg aacgtacggt attgattgct aatacatcaa
    33421 acatgccagt agcagctcga gaagcttcta tctatacggg aatcacaatt gcggaatatt
    33481 tccgtgacat gggttatgac gttgctatca tggcagactc cacttcacga tgggcagaag
    33541 cacttcgtga gatgagcgga cgtttggaag aaatgcctgg tgatgaaggt tatccagctt
    33601 atcttggttc acggcttgcg gaatactatg aacgatctgg aagagtcatt gcattaggat
    33661 cagaaggtcg cgaaggaagt ataaccgcaa tcagtgctgt atccccgtct ggaggggata
    33721 tttcggaacc agtcacacaa aatacattgc gggtcgtaaa agtattctgg ggtcttgatt
    33781 ccagtttagc ccaaaaaaga cattttcctt cgatcgattg gatacaaagc tattctcttt
    33841 attcaacaga agtagggaag tatatggatc atatccttca agctgactgg gcatccatgg
    33901 tcaccgaggg catgcgtatt ttgcaggaag aacagcagct gaatgaaatc gtgcgtttgg
    33961 tagggattga ttccctttca gataatgacc gtctgacatt agaagtggca aaatccattc
    34021 gtgaagatta tttgcagcaa aatgcttttg atgatgtcga tacattcact tcaagagaga
    34081 aacaattcaa aatgttgaag atgatcttga cctttggtga agaaggacgc cgtgcgttgt
    34141 cgttaggtgc ttatttaagt gagatcatgg acggtaccgt atcgattcgt gaaaggatcg
    34201 gacgaagcaa atatatcccg gaaacagaat tagaaaaact agatcaaatt attggtgaaa
    34261 tcaagcaaac gatgcaaacg atcgtttcag aaggaggcat gagcaatgat taaagaatat
    34321 cgtacgatcg gtgaagtcgt cggtccattg atggcggttg aaaaagttgc tggggtcaaa
    34381 tatgaagaat taatcgaagt ccgtatgcaa aatggcgaga ttcgtcgtgg acaagtattg
    34441 gaagtccaag aagataaagc aatggtccaa atattcgaag gaacaagtgg tatcaattta
    34501 cgtgactctt ctgtacgatt tttaggccat cctttagaac taggtgtatc ggaagacatg
    34561 atcggtcgag tattcgatgg tttgggacga ccgaaagata acggaccaga gatcctgcct
    34621 gaaaagcatt tggatattaa cggagaagta atcaatccag tagctcggga ttatccagat
    34681 gagtttatcc aaacaggtat ctctgcaatc gatcatttga atacattagt tcgtgggcaa
    34741 aaattaccag tattttctgg ctctggtctt cctcataaag aattagctgc tcagatcgcg
    34801 cgacaagcaa cggtactcga ttcatcagat gatttcgccg tagtctttgc tgctattgga
    34861 atcacttttg aagaagctga atttttcatg gaagattttc gtcaaacggg cgcaatcgac
    34921 cgttctgtca tgtttatgaa tttagcaaac gaccctgcaa tcgaacgtat cgctactcct
    34981 cgtatggctt tgactgctgc agaatatctt gcttatgaaa agggaatgca tgttttggtt
    35041 atcatgacag acatgaccaa ctatgcagag gcattgcgag agatttctgc agcacggcga
    35101 gaagttccag gaagaagagg atatcctggg tatctttaca cgaacttagc tactttattc
    35161 gaacgagcag gaagaatccg cggactaaaa ggatcggtta ctcaaattcc gattctaaca
    35221 atgccagaag acgataaaac tcatccaatc cctgacttga ctggttatat tacagaagga
    35281 cagatcatct tgactagaga gctttacaag agtgggattc agccaccaat tgatgtactc
    35341 ccgtctcttt cgcggttaaa agataaggga actggacccg gaaagacaag agaagaccat
    35401 gcagctacga tgaatcagct ttttgcagcc tatgcacaag gaaaacaggc aaaagaatta
    35461 gccgtagttt taggtgaatc tgctttatct gatgtagaca agatctatgc gaagtttgct
    35521 gagcggtttg aaaaagaata tgtcaatcaa ggattctaca cgaaccgttc gattactgaa
    35581 acacttgatc tagggtggga actgctatcg atgttgccaa gaaccgaatt aaaacgaatc
    35641 aaagacgaca tgctggacaa atatctgcca gaagggaagt gagttctgat ggcacgattg
    35701 aatgtgaatc cgacccgcat ggaattgact cgattgaaaa aacagttgac aactgccaca
    35761 agagggcata aattattaaa agataagcaa gatgaactga tgcgtcaatt catcctgttg
    35821 attcgaaaga acaaccagct gcgtcaagaa atggaagagt ctatgacaga agcaatgtct
    35881 gattttgtat tggcaaatgc ttcgatcacg gaacctttca tcgaagaatt atttgttttg
    35941 ccggcggaaa acgttgattt gtctatcgta gacaaaaata tcatgagtgt aaaagtacct
    36001 gtgatgaatt ttgaatatga tgaacagctg acagacacac ctttggaata cgggtatttg
    36061 aattccagtg ccgaactgga tcggtcaatc gatcggttta ctaccctgct tccaaaactt
    36121 ttggaactga cagaaatcga aaaaacttgt caactgatgg cgagtgaaat cgaaaagaca
    36181 cgtcggcgag tcaatgcatt agaatatatg acgatccctc aattagaaga aacgatctat
    36241 tatattcgta tgaagctgga agaaaacgag cgcgcagaag taacaaggtt gatcaaagta
    36301 aaaaatatgg gtaccactga ctaagtggta tcttttttta aaagaggaaa acgatgatta
    36361 aacaaagcaa acaaataaag aaacgtttgc cggctgttca gcttctcgca ttaggatttt
    36421 ttctcctgat tttagttggc ggcggcctac ttaccctacc attttttagt aatagtgggc
    36481 atgggacaaa ttttatcgat gcgcttttca ctgcaacttc tgctgtctgt gtaacgggat
    36541 tgacaacatt gaatacagca gagcattgga acagtgctgg tcaatttttg atcatgcttt
    36601 tgattgagat cggcggtctt ggttttatga tggttccgat tctatgcttt gcattgataa
    36661 agaagaaagt cagtctcagc acacgtatta tattaaaaga agcgctgaat ctagaagaag
    36721 tatcaggtgt gatgaagctc atgctttaca tcctaaaatt ggcctttgtg atccaattga
    36781 ttggtacggt tgctttatct tttgtatttg tgcctgaatt tggctggttg aaaggtatct
    36841 ggtacagttt gttccatgca atctctagtt tttgtaatgc agggtttgat ttgttaggag
    36901 acagtttagc caatcaccag caaaatgttt atttgatcat ggtggtatct gctttgatca
    36961 tttctggtgg gttaggcttc atcgtatggc gtgatttact ggaatatcat cagaaaaaga
    37021 agatgacgat tcattcaaaa atcgcattaa ccgttacagg taccctttta ttaggcggat
    37081 tcgttgtgtt ctttttcaca gagcgcaatg gggcaacgct agtggaaggg aatatgtttc
    37141 aacgattggc aaacaccttt tttatgagtg tgacacctag aacggctgga tattattcag
    37201 ttgattacct ccatatgagc catgcaggtc tgattttaac gatgtttttg atgtacatcg
    37261 gcggaacatc tgggtcgacg gccggcggat tgaaaacaac tacgttaggt gtgttgctga
    37321 tccagatgcg ttcgatgttg aaaggacgta cgagagcgga agcatttggc cgtaccatcc
    37381 gacaaggcgc agtgttgcgg gcacttactt tattttttgt tacgttgacg ttgtgtgtag
    37441 tcgccatcat gattttatcg gtgactgaaa cgatacctaa aacttctggc atcgaatata
    37501 ttgcttttga ggtcttctct gcgtttggta cagtaggtct tacgatggga ttgacgccag
    37561 acttgactgc aatagggaaa ttgatcatta tagcgcttat gtacattgga cgagttggtg
    37621 tattgactgt ggtcttctct ctgatgacaa aagcacaaag aacacaatca aaatacaaat
    37681 atccagaaga aagtgtcatg attggataat ttcatcttct gacagaaacc tagtgattta
    37741 gtaaaataag aaaataaaat aatttttctt gcttcttgga tactttaagt gcaaaatgtt
    37801 cgtttatttt ttgaggcact acccgttgtt gaacctcggg tagtgcctct tttttattga
    37861 aaggaatcaa taagatttga ttcctttatt tatcaatagg ttataattca tattttttta
    37921 ttttttcttt tacgatactt agtccagaag ctaaaatttc aaggttatta gaggataggg
    37981 aaattcgaac gaatgcatct ggttgcgcac gtgtagtaaa gcgtctggaa tgataaagtc
    38041 ggataccgtt agcaagaaag tcattctcaa ttatcttagg gtcaatctca ggatcaactg
    38101 gaagaattcg atagtaaggg acacgattcg ttggtttttc tactttgaaa agagaatcaa
    38161 aaagctgatt ggctcttttt gtaagactta atttagtttc taagatccta tttgcttcat
    38221 ttgaatggag gatttgagtg atgacttcca catctaatcc agaagttttc acatttatat
    38281 taaaaattgc ttgttctatc ttgctttgga catgtactgg aactacaaga taagcaactc
    38341 gcaatccaga acaaataaat tttgtcattc cagctaagta gattgtttgg ttaggtaata
    38401 aattttggaa aggaggaatg atcgtatgtt gatagtaagt tgttaaaaat gaatggatat
    38461 catcttcaat aacccataga ccttcttttt caatcacttc ggctaattgc tctcgtcttt
    38521 tgtgagagat ttgaaaccct ataggattat tacaagaagg cataagaaag acaccatgaa
    38581 tcttcttttt tctacattcc tgaatcaata tatcaggtcg cattccttgc tcgtcaaaat
    38641 caattggaac gatctctaaa tgatataact gagccaattc aatgaaatta gcgtatgtat
    38701 atcggtctgt tgctattcta tcacctggag agaaaagtgc agaaagtgtg atagccaatc
    38761 cattttgaac accggaaaca atagatagag attctaagga cggaatgact ccttgagttt
    38821 ttaaccaatt tgctgcagta gtcaattgaa aatttgtgcc tttgggatct tggtaattaa
    38881 gcaaaccaac aagatttggg ttattactag ttgattgaat aaacgggaca acgaatttat
    38941 tacattcttc aaaagaacta accagaccaa aatcaatgat atgttcggtt acttcattgg
    39001 tagagatggt gatggactca aagacattgg gagaaataaa tgtgccactt ccaatattcg
    39061 tatacagcaa acctttctca attccatatt tataagcctt gccaacggta gtaaaattta
    39121 aatctaaata atcagctagc tctctttggg agggtaactg agtgtttttc tgtaatttat
    39181 tctttaaaat atcttgttct aattgttgaa tcaacgatcg ataataagga cgcttcaatt
    39241 tttgtttgtc tggtcgccaa gttaacgggt aattgtcata agaattaata ggcattttct
    39301 ccactcctta aaattgtata ccatacaatt ttaccattga ttgtattgat tttagaagtt
    39361 aatcttttgt gaggagggaa ttgtctatga aaattattaa ttttgaggaa gtagtagctt
    39421 cttttagttt tgaagaagcg attcaagtaa tgaaaaaatg tttttttgat tatgaaagtg
    39481 gtaaaatcag ccaaaatcct cgattggtgg aaattttacc tgacggaaaa ggcaaaaata
    39541 tgtttgcgat gatgcctgct tatttggggg agaacagata ttttggcaca aaagtcatta
    39601 ccgcttttcc ggataatcat cagtatcatt taccttctca tttaggagag attttcttgt
    39661 ttgatgctca aaacggacta cccaaagcat tgataaacgc aaatgcagtc acctggattc
    39721 gaacagctgc tgtatctgcg ctggcaacag attacttagc aaatccgcat gcagaaacac
    39781 ttgctttaat tggagctggt caacaagcat tgtcgcattt gactgcgatt tctaccattc
    39841 gtcccatcac tacagtgttt gtttacgata ttttagaaga aagacgaaat acttttatcg
    39901 ctaaagctaa aaaacagtat cctaaaataa cgtttataaa tgcaatatca gtagaagatg
    39961 cagtacgtcc agcggaaatc atttgtacgt tgacctctag taaagaggca tttcttcaaa
    40021 aagagtgggt aagggaagac gcacatatca atgctgtggg cacatttact cctgatacaa
    40081 gagagataag tacatcgttg atggaaactt ctcaaatcta tgtagatgat tactcagctg
    40141 ccttaacaga aagcggggac ctgcttatag ccatggcaga aggagattta gaggaatctt
    40201 gtattgttgg tacattaggc gaattagttt ctgagaaaaa aacattttat aaaggagaat
    40261 cgaggccgac gattttcgac gcagttggtc tagctattga agatctttgt tgtgcagaat
    40321 atatttataa caaattagag aaaggggctt taaaatgaac ttagattatt acgtagttga
    40381 tgcatttgca gatgaggtgt tcaaaggaaa tccagcagcg gtttatgtgc tagaagaatg
    40441 gctgccagaa ggtacgatgc aaaaaattgc cattgaaaac aatttatctg aaacagcatt
    40501 tacagtgaag aaaaaccaag aatttgagct tagatggttt acgccagatc gagaaatcga
    40561 tctttgtggt cacgcgacat tagcaacagc atttgtttta tttaactatt acaaaatacc
    40621 tgatgaaacg attaaatttt ctactcaaag cggtaatcta tttgtgacaa gaattaaaga
    40681 ttattattac atggattttc ctagtattat gccgaaaaaa gtgccgatat tgtctgaata
    40741 tgaagaagcg attggggcta agattaaaga agcctactta gcacgtgatt tatttttcgt
    40801 attagaagat gaaaaaacgg tggctaaatt gcagccagat tttacagcta tcaaaaattt
    40861 tgaattagga ataggagtca tcgtgacagc agaaagtgtg caaaaagact ttgtttctag
    40921 aaccttcttt ccaaaattaa caatcaatga agatccagta tgtggatcag cacattctaa
    40981 tcttattcct tattgggcag aaagaatgaa taaaacaaaa ttgtcagctt atcaaatgtc
    41041 atcaaggggc ggggatctag aatgtgaatt aaaagaagat cgtgtgataa taggaggaaa
    41101 agcagttttg ttttctaaag gtacctcgtt tatttgattt ctttcaaatg aattaaaaat
    41161 aaattggtgg agagtggcaa ccttatagct acaatggttg ttaaaaaaag acgcaaactt
    41221 tttcaattta atgaaaagtt tgcgtctcat tttaattatt ggtgtttgtt ttttattcaa
    41281 tcaagagtaa aatgccccat actaactatc ctcggatgac ttcgatttta taaccatcag
    41341 ggtcaatgac aaagtagtaa gaaggcgcag tgccaggtaa tccttttaag tcagtaatct
    41401 caagaccagc agctttatgt tgctcgtgta atttttctag atcatcagct gctatagcga
    41461 tatgaccata gccgtctcct agatcatacg caccatgatc atagttgtaa gtcaactcta
    41521 attcatagtc atccccagga agtgtcaaat aaacaagtgt aaatttgttt tctggaaaat
    41581 cacggcggcg gctttcttca aaaccaaatg ccttttgata aaattcgatt gatgcttcaa
    41641 gatctttcac tcgaacacat gtgtgagcca ttttcataga ttcatctctc cttttccttt
    41701 tactcaatca taacaaaaag aagtaaggaa atagttgatt tgtgcttgtt catgagaaat
    41761 ttctcatgta aaatggatag aaaataaaac tgttaaggtg gaaactattc atggataaac
    41821 caatttttgg tgaaaaaaag caatttgctg attatcaaac ccgttatgca gcatatattg
    41881 tcatagaaag agatcagcag attgcagtaa tcgaggctcc aaatggtgct ttttttcttc
    41941 ctggcgggga aattgaagga gaagagacga aagaagaagc gattgtccgt gagctgatag
    42001 aagaaatggg aattgtggca gaaatttctt cttatctggg agaagctgat gagtattttt
    42061 actcgaatta tcgtgcaact tattattata atccaggata tttctttgtt gctgattctt
    42121 ggaagcgtat aggagaacca actgaaaaga ccaataaaat ttggtgggtg agccctcaag
    42181 aagcgcttgt gaaattgaaa cgtggtagcc accgatgggc agtcgagaga tggatcgaaa
    42241 aaaataaaaa ctgaaagaaa gcgagtagat cagctttctc tcagtttttt tatttattac
    42301 agtacatatt ttttgattgc ttcagctaca ccgtgctcgt cgtttgatga agtgatgaca
    42361 tttgctgctt ctttgacaag agaaacggca tttcccattg ctactcctag accagcatat
    42421 tcaatcatgg ataagtcgtt ctcgttatct ccgatagcca tgatttcatc ttgatcaata
    42481 ccaaggtgct gtgcgagatt cgcaactgcc gcacctttac ttgcttcttt atttagtact
    42541 tcgaagtaat agggcgcact tttgaccgtt gtgtatttat cacggaaagt ttgcggtaaa
    42601 cgcgcaattg ctgcatctaa aatttctggt tcgtcgatca tcatcatttt gacgatgttc
    42661 atgtctggtg tcatttcttc aggtgttcga tattttagcg gcatcttgac taaacttgct
    42721 tcgtggatcg tataagcgct gatatctcga ttaggcgtgt agatcgcttt gtcatcgatc
    42781 gaatggagat gactgccgac acggcgtgcc attacttcga tctctaggaa atcttcatgt
    42841 gtcagtccgt gacgggcgat gattttacct gatttcgttt gttgtacgag tgccccgtta
    42901 taagtaataa cgtaatcatt atcttgaaaa aggttcaact cgtttaactg ttcctggaca
    42961 cctggtaagg gacggccagt gcataaaacg atcgaaacac cttttgcaac agcagcggca
    43021 atcgcttctt ttacttcttt tgttacttct cttttctcat ttaataaagt accatcgatg
    43081 tcgatagcga ctagtttgat agacataatt tcctccaata tattttaatt tatttcaatc
    43141 aatgcgccat tacggatatg gctggcaaat tcttgataag tttcgtcaaa taaatcataa
    43201 tgatcacgca aggacggatc aaccatttct tttggaaaga aaaatcgctc atctccttgt
    43261 tcttgtcccg ctaatgcagc aacaagctga ctaacctgtg ataattctat caaagttccg
    43321 tcatttcgca aaatctcgat ttgtgtgcga tgtctgcctt gttctgggcg ataaaaatca
    43381 taaggtagat cgtagctaga attcacagca gtgtaataaa caggattata tccgaccttt
    43441 tctactaaca aagtgagttc ttggactaat tttgaatccc ggttgccaga aaaagtagca
    43501 gatttcagtg gtttacgatt caaaaaacgt ttagctaaat catttaaaat aggatcagca
    43561 gattcagacc attgagtaaa gtaagtgccc aagaccccat catcaagttt gaggtattct
    43621 tctaaagtaa agcttccgtc aagaaatggc agaagtaatt gagaatggag ttcaaaactg
    43681 ctggcatctt cttgatacaa ctcgtgtgcg cgatgaagca gatgctccaa aattacttcc
    43741 ataccacggg aaacaggatg gaagtacact tgcacataca tttgatatcg gctaacgata
    43801 taatcttcca ctgcatgcat gccactcatg gaaaaagcga tcccgccttc ataaggacga
    43861 atcacacgta atattcgggt aagatcaaat gttccatatt ctgttccagt gaaataagca
    43921 tcccgaagca aatagtccat tcgatctgca tcgatctgac tagaaatcat ttggacaact
    43981 tgtgggttgg ggtaagtttt ttggatcaca ctagctacct tttcaggaaa accttcttct
    44041 actcggttaa gaatctgata cacttctgtt tctggagaag tgatgatctc tacagtgatg
    44101 gcttcatgat tcgtatggaa gatatgttca aatgtatgag agtaaggacc atgaccgaca
    44161 tcatgcaaca gtgccgcaca taaagtcacc agccgttcct gatcatccca gcctccgttg
    44221 cctatttttt cttttgaaaa atttcgagaa aagatatcgc agattcgtct tgaaatctca
    44281 taaacgccta atgaatgagt aaaacggcta tgttctgctc cgtgaaaagt gaaagaagaa
    44341 gtgcctaatt gtttaatgcg tcgtagacgt tggacttctt tcgaattgat caaatctaag
    44401 attacttgat gctggacatg gatatagtta tggactggat cacgaaaaac tttttccata
    44461 ggcagcattt gatatttata aggggttgtc ataatgtagc tcctttatgt tattacgttg
    44521 ttccattctc tggatttggt tgttccattg ctccagctgg atttcttttt ttagccagtc
    44581 ggtagaatcc tgtaaatcac ttgcagaata cagcttttca aaagcttttg caaatgcttc
    44641 tttgacttct tttacaaaaa ggggttggtc aagcagttct tcaagtgttg ccattgtagc
    44701 aggatccact gcaggatagc catttgtacc aaagtcttct tttaaagcag attgataaaa
    44761 tcgcttcatg acttgcccac gtttttcctg attaccgttg acacttaagt acatcataac
    44821 agaaatccca tttttgatcc gacgctgagc gataccggca aattttttgc catggatgct
    44881 taaatcataa gttccaggac aataagaatg ggtgatttcg aatgcttgta tcggtgcctt
    44941 tggaaaagcg tatttagtca ataggagcat ttgctcgtaa gcgtcatcta tggttatttt
    45001 ttttgtgtca ctttggggga aaatcaaaga aatattcaaa attccttcat ctgatacgat
    45061 ccctaatcca cctgaatttc tgatcactgg agcgtaacct gcatcttcca gtgtttggat
    45121 cccgtcactt agaaaaggag ttcgaacatc cttcatacct aagatgactg tgcagtcata
    45181 ttgccagaaa tgtaaaaaga tttgctggtg ttctttagaa tattccgtta atatatctgt
    45241 taatgcaaaa ggggaaaagt cgtttttttg ataaatgtgc tgatctagtc gaatcgcagg
    45301 gttcattcaa ccggctcctt tgtataaatt catattttct acaatattat actctaaaat
    45361 caaagagttg ttttggaaaa aaggttttct gtaaaaatgg acaagttggt tgacaaaaca
    45421 aaagaaacta ggaacaatgt agctatgaga agtaaaggag aatgaataat ggaattatca
    45481 caaggaacac cgatttcaat caaattgcgt acaaaagtaa aacagcaagg ggaagtgcaa
    45541 gatttttatt ttgatttgaa agggcaaatg gtgacaattg gcgatacgct gtacatacgt
    45601 tataaagaga ttcaggagga gacgggcgat gagattcctg taacgatcaa attagttcca
    45661 gatggtcatg tacagctgat ccgtgcagga gaaatgcgga tgcgtttgaa atttggctat
    45721 aaagaacggt tagaaacatc ctatcgaaca ccatacggta tgatccagat tgctactttt
    45781 acgaaagaac tgcatgttag tttgaaagac cgtcccacag cagggaaggt tcgaatcgat
    45841 tatgatctgt tcatgggacc ggaaaaaatc ggcgaatatt atttgacctt ggattttaca
    45901 gcgtaaatat aaaagaaaag tttgattttt tgtgtctgat tttgtatgat aaggagtaga
    45961 ctgtgaaagg acgtgtcctg attggaaatt agtgtatttg aaggtgcaaa caaaagcgaa
    46021 ttatcaatga tcgaagtagc acacgcgatc ttagaacaac gtggcgatgt catggatttt
    46081 tctgatttag ctaaccagat ccaaaactat cttgggaaat cagacagtga aattcgtgat
    46141 tcactcgcac aattttatac tgacttgaat attgacggca gctttatctc attaggggat
    46201 aatcgttggg gcttgcgttc atggtatgct atcgattcga tcgatgaaga agtaaaccat
    46261 ggaatggacg aagaggatga agatactcca cgtcgccgta aacgcaagaa agtcaacgcc
    46321 tttatcaatg atgatgaaga cgcgatcgat tacaatgacg atgacccaga agatacagat
    46381 ctgacagaag aagatgacga tgatctgttt gatgacgatg atgatgaaga tgaagaaatc
    46441 gctgcttata attctgactt gcaagaaatc ggagcagatg acccgacaga tgatgaagaa
    46501 gacttgccag gaaatatcga agaagattta agtatcatag acgaagacga tgatgatgaa
    46561 gattatgaag aggaagaatt ttctgactct gaaaaagaat aaacattatt ttcttacgag
    46621 aggttgtgac agagacgatc agcctatcag aataaggagg tgtgacacga tttttgtcac
    46681 accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa aaattgaaac
    46741 ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga atgattttta
    46801 ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat ggactcatcg
    46861 aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat aaagaagagg
    46921 ccttagatgt gatgcaagat agttttcata aagccctgaa ctattttgaa aaagaaaaag
    46981 agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca ttagatagtt
    47041 ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc ccccaacagt
    47101 tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata gacagtccgg
    47161 aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa attgcgacta
    47221 tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg aatgctttac
    47281 gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga aaatttaaaa
    47341 aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga ggagatccgc
    47401 cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa agcggagaaa gagacgtctc
    47461 caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct tctttttaat
    47521 agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat tgaattgatc
    47581 ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc gaagatcaat
    47641 acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata ctttcgcgaa
    47701 ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac ggatcattac
    47761 caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt gattgaaagg
    47821 cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac aatcgacaag
    47881 aaaaacagtg tccagctctc acttccactt ttattcaaag atgacaccta tctatctgtt
    47941 ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc atctaagtac
    48001 tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact tgttacgcct
    48061 acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc tcaatacgaa
    48121 attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt gacaaaaacg
    48181 attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga ctagcagttt
    48241 caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga agagatatag
    48301 tgaaaagaaa ttatttagtt atcagtatta ttgctttatc cgtgcttttg tttttcgaaa
    48361 ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct tcggagcatt
    48421 cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca actgaaacag
    48481 ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcag tcatcacaat
    48541 caacacaaga cagtacaaaa acgagccgag atccggctgt caaaagcgtc gcaatcagct
    48601 ttgatgatgg accaggagcg accacaacac cgcagcttct gcgaatctta aaagaaaaaa
    48661 acgtgcatgc cacttttttc gtcttaggag aaaatacggc gcagcatcct gaaatcgtga
    48721 agcagacagc tgaagcaggg cacgagattg gcaatcacac atacgatcat cagaatctgg
    48781 ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact gagatcaaaa
    48841 aagtcactgg caaaaccccc acttttgttc gtccgcccta tggttctgtc acgaatgtag
    48901 gagcaacgat catccagcga ccgatcatcg aatggtctgt tgattcagaa gattggaaga
    48961 caagaaatcc tgatttgatt ttgcagaaga tccaagcaac agtatacgat ggggcaatta
    49021 ttttattcca tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg atcgattatc
    49081 tgaaagagca agggtatcgc atcacgacag ttggggattt attagggcat ccaacggctg
    49141 tcgagaacta ttatggcaga aacgatcatc ggccagttca gtaagtgaga caagtatctt
    49201 taataaataa cacattttaa taagtagttg aacacataac taccttactt gcaaaggctt
    49261 aacaatatct atacaggtac aacaaaaaac tgtccaccaa ttttctttgg cggacagttt
    49321 tcacttaaaa ataaagaagc aactattcgt aaattatgct aaaatagcag tagaacgcgt
    49381 cctgagggaa tcgaaccccc gtctcaagaa ccggaatctt acgtgatatc cactacacta
    49441 aggacgcaag cactgggata attgtaacta aacagataaa aaattacaag gggattggac
    49501 aaaaaaatga aatttgagca aaatttttca caaaaacagc agcagacaca gaaactagcc
    49561 atgacgcaac agttgcaaca gtccatccaa gttttacaat ataacagtga ggagctgttg
    49621 gcattcgttg agaatcaagc aatggaaaat ccattggtgg aagttgttga gccggaatgg
    49681 cagccagatt acataaaagc ttcttcatct tcttatgaag gagaagagac aaattaccta
    49741 aatcagattc ctgatacaaa agggtcttta tttgattcat tgatcgaaca agtccatttg
    49801 aattaccgag atactttttt gaggaaaatc gttctttatc ttgtagaata catcgatttg
    49861 aacggatttt tgacgattag tttagaagaa gccggaaaag agattggagc tacgcctatc
    49921 caaatgctcg atgcgttgac tctgattcag caattagaac cagctggagt aggtgcacgt
    49981 agtttgcaag aatgtctcat gcttcaaaca gaacgagatg attatgcgcc ggaattagct
    50041 tatatcgtgt tggaagaatg ttttgaggaa ctggtggagc gaaaatggaa agaaatcgca
    50101 caacgttttt ctgttgatct acatgctgtt cagcagatat tcgactattt acaaacgttg
    50161 agtccaagcc ctggccggat tttcgaccgt agcagcgaat tgtacattat tcctgatgtc
    50221 cgggtattag tggacgacga taaaaatgta caagttatct caaaccgaaa aaatcagcca
    50281 aatatacgct ttcaagagag ttacttcaaa caaatgtcac aacaagcaga taaagaaact
    50341 gaaaattatt taaaagaacg gaaacaggaa ttcgaatggt tgaagaaaac gatcctacaa
    50401 cgtggggata caattttgcg ggttgctcaa gtcattgttt cccggcaaaa agctttcttt
    50461 atcgacaagg aacgtccaat caagcctctt acgttaaagg aagttgcaac tgagatagat
    50521 gtgcatgaat ccacggtcag tcgtgcagtc aacgggaaat atttggaaac agacttcggt
    50581 gtatttgaat taaaaaaatt tttcactaca cgaattccta cgaatagtac agaacagaca
    50641 gaagaccttt cagcagatac agcgaagaaa aagctgcagg aattagtcga tcaagaagac
    50701 aaaaacaagc cgttgtctga ccagaaattg gttgaattgc tgaaaaaaga tgagatagcg
    50761 atctcaagac gaacagtagc aaaatatcgt gatttattag gaatcccgag ttcatcaaaa
    50821 agaaaacgtt atgataacta atcagtcaaa agactagaaa aagtatttca ctttttctag
    50881 tctttttagt tgaaaaacga gagcaaaact gttagactta cagtcgtgag gttgagattt
    50941 ccctttattg atgtgaaatt cgaatagtta atttcttttt ttatcgtata tgggtcgttt
    51001 taagtctaca ttggacgtaa agtgtccaac taaggagatc gctatgctgg atgaaataaa
    51061 aatgattgaa tctgttgcac cagatattat cgaagtgttg caagaacgct ataaaatttt
    51121 acgaaatatc tattggatgc agccgattgg acgtcgaagt ttatcggaaa gtatggggat
    51181 cacagaacga gtattgcgaa cagaaacaga tcttttgaaa aatctgaatt tgatcgacac
    51241 ctcaaaaagc ggtatgacgc tgacaactaa gggcgaagaa gtttatcaaa gccttgaaaa
    51301 cttcatggat cagcttttag gtacgcatca aaccgaacaa cagttggcag aatattttgg
    51361 taaccaacgc tgtatcgttg tttcaggaaa cagcgaagag cagaccaaag tggctgatgc
    51421 attcggcgaa gcgctgagcg aagccctaga ccgactactt ccagaaggcg agaatatcat
    51481 cgctgtgatg ggaggaacta ctatggctgt ggtagccgaa caattgtcta atctggaaaa
    51541 taagaaacgg cataacctgt ttgtccctgc aagaggcggg attggtgaag cgataactgt
    51601 acaggcaaat tctgtcagtg caagaatggc tgctaaagca aacggtaacc accgtgcact
    51661 ttatgttccg gaacaattaa gtctggcaac ttacaattct ttactcaatg agccatctat
    51721 ccaagaagtc ttaaacttga ttagtgaagc gaattgtgtt attcatagta ttgggcgtgc
    51781 attacacatg gcagcacgtc gtaagatgac tgaaaaagaa ttagtcatgc tgaaacaagc
    51841 aaatgctgtt gcggaatctt ttggctactt tttcaatgaa aaaggtgaag tcgtttataa
    51901 ggtccctaga atcggcatag aattgcaaga tttggaaaaa gtcccgatca tcatggcaat
    51961 cgcgggcgga aaatcaaaag ctaaagcaat tcgagcttat atgaaaaatg caccaaaaca
    52021 aacgtggctc atcactgatg aagctgctgc aaatgagatt ttaaaagggg taacccttta
    52081 aaataaatat ttttttgatt ttcataagga ggaaatctta aatgacagtt aaagtaggta
    52141 ttaatggttt tggacgtatt ggacgtctag cattccgtcg tatccaagat gtagatggaa
    52201 tcgaagtagt agcaatcaac gacttgacag atgctaaaat gttggcacac ttgttaaaat
    52261 acgacacaac tcaaggacgt ttcaacggaa cagttgaagt tcatgaaggt tcattcaacg
    52321 taaacggaaa agaagttaaa gtattagcta accgcaaccc agaagaatta ccatggggcg
    52381 aactaggcgt agatatcgtt ctagaatgta ctggtttctt cacttctaaa tcagcagctg
    52441 aaaaacattt aacagctggt gctaaacgcg ttgttatctc tgctcctggc ggaaacgatg
    52501 ttccaacaat cgtttataac acaaaccacg aaacattaac tggtaaagaa acagttatct
    52561 caggtgcttc atgtactact aactgtttag ctcctatggc taaaacattg aacgacaaat
    52621 ttggtgttgt tgaaggatta atgacaacta tccacgctta cacaggtgac caaatgactc
    52681 tagacggacc tcatcctaaa ggtgacttcc gccgtgcacg cgctgctgct gcaaacatcg
    52741 ttcctaactc aacaggtgct gctaaagcta tcggtttagt aattcctgaa ttgaacggta
    52801 aattagacgg agctgctcaa cgtgttcctg taccaacagg ttcattaaca gaattagtaa
    52861 cagttcttga aaaagaagta actgttgacg aaatcaatgc agcaatgaaa gaagcttcaa
    52921 acgaatctta cggatacaac acagacgaaa tcgtttcttc agatatcgtt ggtatgactt
    52981 acggttcatt atttgatgct acacaaacta aagtaatgac agttggcgac aaacaattag
    53041 ttaaaactgt tgcttggtac gacaacgaaa tgtcatacac tgcacaatta gtacgtactt
    53101 tagaatactt cgctaactta taagatagac aaacgtacaa gtgtaaatta tagacatgta
    53161 catttaaaag cggggaagca atcgcgcttc ttccgctttt tttgataata agtttattca
    53221 ggaggaaata aaatggctaa gaaaacagtg aaagatatcg atttaaaaga caaaaaagtt
    53281 cttgtccgtg ttgacttcaa cgttcctttg aaagacggcg tgatcactga cgacactcgt
    53341 atcaaagcag cattgccaac aatcaactac gttctagaac aaggcggaaa agcaatcctt
    53401 ttctctcacc ttggacgtgt aaaaacagaa gaagataaag aaggaaaatc tttagctcca
    53461 gttgcaaaac gtctaggcga attattaggt aaagaagtaa cattcgtacc tgaaactcgc
    53521 ggagaacaat tagaagaagc aatccgcaac atgaatgacg gcgacgttgt cgtattcgaa
    53581 aatacacgat ttgaagatat tgacggtaaa aaagaaagca aaaatgatac agaactaggt
    53641 aaatactggg cttcattagg cgatgtattt gttaacgatg ctttcggtac agctcaccgt
    53701 gcacatgctt ctaacgtagg tatcgcttca actggtatcc caacagttgc tggattcttg
    53761 atggaaaaag aaatcaagtt catcggtgaa gcagtagaag aaccaaaacg accaatgatt
    53821 gcaatccttg gtggcgcaaa agtttctgat aaaatcggcg taatcgaaaa cttgattcct
    53881 aaagcagata aaatcttgat cggtggcgga atgacttaca cattctacga agcaaaagga
    53941 atcaagatcg gtaactctct agtagaagct gataaagtgg aactagcaaa agaattaatc
    54001 gaaaaagcag gcgacaaact tgtcttgcca atcgacaatg tctgtgcacc agaattttca
    54061 aatgatgttg aaacacaagt aatcgaagga gacattcctg atggtttgat ggctttagac
    54121 atcggcccag catcagtaaa attatttgct gatacattac aaggtgcaaa aacagttgtt
    54181 tggaacggac ctatgggtgt atttgaaatg agtaactttg ctaaaggtac aatcggtgtt
    54241 tgtgaagcaa tcgctaactt agaaggcgca acaacaatca tcggtggcgg agactcagct
    54301 gcagcagcag aacaattagg atttgctgac aaattcacac acatctcaac aggtggtggc
    54361 gcaagcttgg aattgcttga aggtaaagaa ttaccaggac tagcagcaat caacgataaa
    54421 taaacgacca agtttgctca attctgacga gtaatcgatc aaaaggtgaa gggagttttc
    54481 atgactccct agccattttt caataaatta aggagtgtgt tcctcaatgc gtaaacctat
    54541 tatcgcaggt aactggaaaa tgaacaaaac tgcatcagaa gcaaaagaat ttgctgaagc
    54601 agtaaaaaac aaaatccctt caaatgacaa agttgattca gtcatcggat ctcctgcatt
    54661 attcttacaa gaattaatga catcagctga aggtacagaa ctaaaaatct cagcacaaaa
    54721 ctgctactgg gaaaactcag gtgcgttcac tggtgaaact tcaccagcag ctcttgctga
    54781 tctaggtgtt cattatgtga tcatcggaca ttcagaacgt cgtgaatatt tccatgaaac
    54841 agacgaagac atcaataaaa aagcaaaagc tatctttgca aatggtatga caccaatcct
    54901 atgctgcggc gaatctttag aaacctatga agcaggcaaa actgctgaat ggatcgaagg
    54961 acaaattaca gctggtttag ctgatctttc agctgaacaa gtgtcaaaca tggttattgc
    55021 ttatgaacca atctgggcaa tcggtactgg taaatcagct gacgcacaaa tcgctgacga
    55081 aatttgtgga gttgtacgtc aaacagttga aaaactttat ggaaaagaag tatcagaagc
    55141 agttcgtatc caatacggcg gttctgtaaa accagaaaat attgctgaat acatggcaaa
    55201 agaaaatgtt gacggtgctt tagtcggtgg cgcaagcttg caagcagatt catttttgtc
    55261 attgttagac gctgtaaaat aattgttgaa aatttgcgaa aaatgcaaga aatgtttgca
    55321 ctaatgagca aaattaacta aaatcgtata tgagggaaat cccttaggaa aaaacaaact
    55381 caaaggagag acaaaacatg tcaattatta ctgatgttta cgctcgcgag atcttagact
    55441 cacgtggtaa cccaacaatc gaagtagaag tatacacaga aagcggagct tttggccgcg
    55501 gaatggttcc ttcaggtgct tcaactggtg aatacgaagc agttgaatta cgtgacggag
    55561 acaaagctcg ttatggcggt aaaggtgtaa ctaaagctgt cgacaacgta aacaacatca
    55621 ttgctgaagc aatcattggc tacgatgttc gtgaccaaat ggctatcgac aaagctatga
    55681 tcgctttaga cggaactcct aacaaaggta aattaggtgc gaacgctatt ttaggtgttt
    55741 ctatcgctgt tgcacgtgca gctgctgact acctagaagt acctttatac cactacctag
    55801 gcggattcaa cacaaaagta ttgccaacac caatgatgaa catcatcaac ggcggatcac
    55861 atgctgataa ctcaatcgac ttccaagaat tcatgatcat gcctgttggc gctccaacat
    55921 tcaaagaagc tctacgtatg ggtgctgaag tattccacgc attagcttca atcttgaaag
    55981 ctcgcggatt agcaacttct gttggtgacg aaggtggatt tgctccaaac cttggttcaa
    56041 acgaagaagg ttttgaagta atcatcgaag caatcgaaaa agctggctat gtacctggta
    56101 aagacgttgt tcttgctatg gatgctgctt cttcagaatt ctacgacaaa gaaaaaggtg
    56161 tttacgtact agctgactca ggcgaaggcg aaaaaacaac tgacgaaatg atcaaattct
    56221 acgaagaatt agtttctaaa tatccaatca tctcaatcga agatggatta gacgaaaacg
    56281 actgggacgg attcaagaaa ttaactgacg tattaggcga caaagttcaa ttagttggtg
    56341 acgacttgtt cgtaacaaac actcaaaaat tatctgaagg tatcgaaaaa ggaattgcta
    56401 actcaatcct tatcaaagtg aaccaaatcg gtacattaac agaaactttc gaagctatcg
    56461 aaatggctaa agaagctggc tacacagcag ttgtatctca ccgttctggt gaaacagaag
    56521 attcaacaat ctctgatatc gctgttgcaa caaatgctgg ccaaatcaaa actggttctc
    56581 tatcacgtac tgaccgtatt gctaaataca accaattact acgtatcgaa gaccaacttg
    56641 gtgaagttgc agaatacaaa ggtttgaaat ctttctacaa cttaaaaaac aaataattct
    56701 tttctaatga agatacatga tcgactattg aaagatagtc gagtatgatc acttagttaa
    56761 gtaatcgaac tctctacttg ctgaaatatc agcaggtaga ga
//
LOCUS       DACCCS010000012        56387 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.012, whole genome shotgun sequence.
ACCESSION   DACCCS010000012 DACCCS010000000
VERSION     DACCCS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.012"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            330..1175
                     /locus_tag="IVK82_000760"
     CDS             330..1175
                     /locus_tag="IVK82_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6391840.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="IVK82_000761"
     CDS             1456..2676
                     /locus_tag="IVK82_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP6391841.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="IVK82_000762"
     CDS             2673..5393
                     /locus_tag="IVK82_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6391842.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="IVK82_000763"
     CDS             5377..6321
                     /locus_tag="IVK82_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6391843.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="IVK82_000764"
     CDS             complement(6390..7400)
                     /locus_tag="IVK82_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6391844.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="IVK82_000765"
     CDS             complement(7580..7924)
                     /locus_tag="IVK82_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP6391845.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="IVK82_000766"
     CDS             complement(7917..8351)
                     /locus_tag="IVK82_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP6391846.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="IVK82_000767"
     CDS             8489..9226
                     /locus_tag="IVK82_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6391847.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="IVK82_000768"
     CDS             9219..10430
                     /locus_tag="IVK82_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6391848.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10637..11416
                     /locus_tag="IVK82_000769"
     CDS             10637..11416
                     /locus_tag="IVK82_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP6391849.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="IVK82_000770"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="IVK82_000770"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP6391850.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IVK82_000771"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IVK82_000771"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP6391851.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="cpaF"
                     /locus_tag="IVK82_000772"
     CDS             14132..15061
                     /gene="cpaF"
                     /locus_tag="IVK82_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP6391852.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="IVK82_000773"
     CDS             15018..16067
                     /locus_tag="IVK82_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP6391853.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="IVK82_000774"
     CDS             16064..16342
                     /locus_tag="IVK82_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP6391854.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="IVK82_000775"
     CDS             16317..16787
                     /locus_tag="IVK82_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP6391855.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="IVK82_000776"
     CDS             16804..17106
                     /locus_tag="IVK82_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391856.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="IVK82_000777"
     CDS             17123..17494
                     /locus_tag="IVK82_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP6391857.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="IVK82_000778"
     CDS             17466..17825
                     /locus_tag="IVK82_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391858.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="IVK82_000779"
     CDS             17997..19004
                     /locus_tag="IVK82_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6391859.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="IVK82_000780"
     CDS             19029..20213
                     /locus_tag="IVK82_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP6391860.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="IVK82_000781"
     CDS             complement(20650..22233)
                     /locus_tag="IVK82_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6391861.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="IVK82_000782"
     CDS             complement(22811..22954)
                     /locus_tag="IVK82_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP6391862.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23177..23656)
                     /locus_tag="IVK82_000783"
     CDS             complement(23177..23656)
                     /locus_tag="IVK82_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6391863.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23729..24013)
                     /locus_tag="IVK82_000784"
     CDS             complement(23729..24013)
                     /locus_tag="IVK82_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391864.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24091..24285)
                     /gene="ssrS"
                     /locus_tag="IVK82_000785"
     ncRNA           complement(24091..24285)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVK82_000785"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24357..25643)
                     /locus_tag="IVK82_000786"
     CDS             complement(24357..25643)
                     /locus_tag="IVK82_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP6391865.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25781..26452
                     /locus_tag="IVK82_000787"
     CDS             25781..26452
                     /locus_tag="IVK82_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP6391866.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26418..26906
                     /locus_tag="IVK82_000788"
     CDS             26418..26906
                     /locus_tag="IVK82_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP6391867.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26919..27866
                     /gene="prmA"
                     /locus_tag="IVK82_000789"
     CDS             26919..27866
                     /gene="prmA"
                     /locus_tag="IVK82_000789"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP6391868.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27867..28631
                     /locus_tag="IVK82_000790"
     CDS             27867..28631
                     /locus_tag="IVK82_000790"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP6391869.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28864..31077
                     /locus_tag="IVK82_000791"
     CDS             28864..31077
                     /locus_tag="IVK82_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP6391870.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31088..31534
                     /locus_tag="IVK82_000792"
     CDS             31088..31534
                     /locus_tag="IVK82_000792"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP6391871.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31705..32964
                     /locus_tag="IVK82_000793"
     CDS             31705..32964
                     /locus_tag="IVK82_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP6391872.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33053..34000)
                     /locus_tag="IVK82_000794"
     CDS             complement(33053..34000)
                     /locus_tag="IVK82_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP6391873.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34394..35686
                     /locus_tag="IVK82_000795"
     CDS             34394..35686
                     /locus_tag="IVK82_000795"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP6391874.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35701..37470
                     /gene="aspS"
                     /locus_tag="IVK82_000796"
     CDS             35701..37470
                     /gene="aspS"
                     /locus_tag="IVK82_000796"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP6391875.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37579..38454
                     /locus_tag="IVK82_000797"
     CDS             37579..38454
                     /locus_tag="IVK82_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6391876.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38720..40603
                     /locus_tag="IVK82_000798"
     CDS             38720..40603
                     /locus_tag="IVK82_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP6391877.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40678..40962)
                     /locus_tag="IVK82_000799"
     CDS             complement(40678..40962)
                     /locus_tag="IVK82_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP6391878.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40952..41686)
                     /locus_tag="IVK82_000800"
     CDS             complement(40952..41686)
                     /locus_tag="IVK82_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP6391879.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41815..42438
                     /locus_tag="IVK82_000801"
     CDS             41815..42438
                     /locus_tag="IVK82_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP6391880.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42630..43751
                     /locus_tag="IVK82_000802"
     CDS             42630..43751
                     /locus_tag="IVK82_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP6391881.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43758..46886
                     /locus_tag="IVK82_000803"
     CDS             43758..46886
                     /locus_tag="IVK82_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP6391882.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46905..50099
                     /locus_tag="IVK82_000804"
     CDS             46905..50099
                     /locus_tag="IVK82_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP6391883.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50245..50952)
                     /locus_tag="IVK82_000805"
     CDS             complement(50245..50952)
                     /locus_tag="IVK82_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP6391884.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51717..52115
                     /gene="spxA"
                     /locus_tag="IVK82_000806"
     CDS             51717..52115
                     /gene="spxA"
                     /locus_tag="IVK82_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP6391885.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52447..53100
                     /locus_tag="IVK82_000807"
     CDS             52447..53100
                     /locus_tag="IVK82_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP6391886.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53326..54324
                     /locus_tag="IVK82_000808"
     CDS             53326..54324
                     /locus_tag="IVK82_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP6391887.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54500..56308
                     /gene="pepF"
                     /locus_tag="IVK82_000809"
     CDS             54500..56308
                     /gene="pepF"
                     /locus_tag="IVK82_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP6391888.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18554 a         9728 c        11686 g        16419 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta
    23041 ttttacttga actcattgac tgttttccct ttattttaaa cattataaag accgatgagt
    23101 gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg
    23161 gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat
    23221 cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac
    23281 caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt
    23341 gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag
    23401 aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct
    23461 ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag
    23521 aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga
    23581 ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt
    23641 tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact cttttattgt
    23701 agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa
    23761 atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa
    23821 agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc
    23881 cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat
    23941 cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta
    24001 aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca
    24061 agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct
    24121 gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca
    24181 aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg
    24241 ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc
    24301 atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag
    24361 ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt
    24421 tcatatttcc ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa
    24481 tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg
    24541 ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct
    24601 ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt
    24661 ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc
    24721 aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa
    24781 cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc
    24841 tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt
    24901 tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt
    24961 ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa
    25021 ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt
    25081 gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg
    25141 cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt
    25201 ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc
    25261 tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc
    25321 tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt
    25381 tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg
    25441 cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca
    25501 ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt
    25561 tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg
    25621 cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt
    25681 atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct
    25741 tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat
    25801 agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta
    25861 tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata
    25921 cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg
    25981 ggcaatgtat gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt
    26041 gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc
    26101 cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag
    26161 caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc
    26221 tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa
    26281 tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt
    26341 tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg
    26401 gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg
    26461 aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg
    26521 agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag
    26581 ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc
    26641 agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag
    26701 ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag
    26761 ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc
    26821 actgggataa tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata
    26881 cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag
    26941 tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga
    27001 gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg
    27061 agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt
    27121 ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat
    27181 tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa
    27241 gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga
    27301 cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac
    27361 tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag
    27421 cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg
    27481 tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg
    27541 aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc
    27601 atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga
    27661 acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa
    27721 caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca
    27781 tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta
    27841 ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat
    27901 caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga
    27961 tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa
    28021 tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa
    28081 aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt
    28141 ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag
    28201 cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga
    28261 ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg
    28321 aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg
    28381 aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac
    28441 cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg
    28501 attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag
    28561 aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt
    28621 ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt
    28681 caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa
    28741 agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa
    28801 ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt
    28861 cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac
    28921 atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat
    28981 gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt
    29041 atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt
    29101 gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct
    29161 atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag
    29221 ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg
    29281 gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag
    29341 caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg
    29401 atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag
    29461 tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt
    29521 ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat
    29581 ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc
    29641 aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat
    29701 gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat
    29761 attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca
    29821 ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg
    29881 gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg
    29941 acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct
    30001 tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca
    30061 ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt
    30121 atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag
    30181 cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt
    30241 ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa
    30301 cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc
    30361 aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa
    30421 gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat
    30481 ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa
    30541 aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct
    30601 gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta
    30661 attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact
    30721 aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca
    30781 aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag
    30841 tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt
    30901 caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat
    30961 aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc
    31021 gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag
    31081 gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca
    31141 agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac
    31201 gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga
    31261 acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca
    31321 attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg
    31381 accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat
    31441 cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc
    31501 tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa
    31561 ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct
    31621 gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt
    31681 attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca
    31741 gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg
    31801 gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca
    31861 tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa
    31921 ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat
    31981 caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat
    32041 cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta
    32101 tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta
    32161 tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc
    32221 ggaacgttta cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa
    32281 aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat
    32341 gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa
    32401 gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg
    32461 gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac
    32521 ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca
    32581 atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc
    32641 agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca
    32701 gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa
    32761 tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa
    32821 tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc
    32881 tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc
    32941 cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa
    33001 cagtgctgat cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc
    33061 cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt
    33121 ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg
    33181 gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa
    33241 tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg
    33301 gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc
    33361 ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa
    33421 gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta
    33481 catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac
    33541 cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt
    33601 ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac
    33661 gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc
    33721 ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt
    33781 tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg
    33841 tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat
    33901 cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt
    33961 ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt
    34021 ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc
    34081 agatatctct tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat
    34141 gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc
    34201 taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta
    34261 cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa
    34321 tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa
    34381 gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga
    34441 atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta
    34501 taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga
    34561 tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt
    34621 gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt
    34681 cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga
    34741 acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc
    34801 agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt
    34861 ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc
    34921 ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc
    34981 taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca
    35041 aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca
    35101 ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa
    35161 tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc
    35221 aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga
    35281 actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt
    35341 gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt
    35401 cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt
    35461 tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc
    35521 agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac
    35581 gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata
    35641 tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa
    35701 atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt
    35761 acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta
    35821 cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg
    35881 gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga
    35941 cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt
    36001 gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca
    36061 atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact
    36121 aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat
    36181 gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac
    36241 tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa
    36301 ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt
    36361 ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa
    36421 acaacgttct taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta
    36481 atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa
    36541 gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat
    36601 ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc
    36661 ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg
    36721 gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta
    36781 gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc
    36841 attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa
    36901 atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt
    36961 gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga attttcagaa
    37021 gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc
    37081 ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac
    37141 ggttatgaac taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta
    37201 ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca
    37261 gctttagatt acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca
    37321 atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa
    37381 gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg
    37441 aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa
    37501 tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat
    37561 ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg
    37621 accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt
    37681 tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact
    37741 ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat
    37801 atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt
    37861 atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc
    37921 gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg
    37981 ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa
    38041 acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc
    38101 ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga
    38161 aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa
    38221 catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc
    38281 aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca
    38341 cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta
    38401 agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag
    38461 tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt
    38521 ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg
    38581 aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag
    38641 gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta
    38701 tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac
    38761 tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc
    38821 aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt
    38881 gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt
    38941 ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc
    39001 ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag
    39061 cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg
    39121 atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta
    39181 tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat
    39241 cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac
    39301 accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg
    39361 gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa
    39421 acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg
    39481 cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag
    39541 tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg
    39601 gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca
    39661 gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt
    39721 gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt
    39781 ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt
    39841 cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc
    39901 aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca
    39961 actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga
    40021 aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca
    40081 agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc
    40141 tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca
    40201 attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac
    40261 tgttctgatc ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat
    40321 ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg
    40381 gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct
    40441 gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca
    40501 tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg
    40561 aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc
    40621 aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca
    40681 tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg
    40741 ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc
    40801 tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc
    40861 attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg
    40921 atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct
    40981 ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt
    41041 ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg
    41101 ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt
    41161 ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac
    41221 ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt
    41281 gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga
    41341 ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga
    41401 cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac
    41461 gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa
    41521 ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt
    41581 gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga
    41641 tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa
    41701 atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt
    41761 caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt
    41821 tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc
    41881 tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt
    41941 acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg
    42001 gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct
    42061 tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg
    42121 aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt
    42181 aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat
    42241 caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc
    42301 gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa
    42361 cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat
    42421 gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc
    42481 ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt
    42541 tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata
    42601 attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata
    42661 tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta
    42721 tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg
    42781 ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga
    42841 atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac
    42901 tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag
    42961 aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac
    43021 caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag
    43081 ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta
    43141 gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg
    43201 ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt
    43261 tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat
    43321 tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc
    43381 tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt
    43441 tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata
    43501 tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc
    43561 ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa
    43621 aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg
    43681 aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg
    43741 aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta
    43801 tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga
    43861 agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa
    43921 cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag
    43981 aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac
    44041 cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa
    44101 cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag
    44161 cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt
    44221 tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg
    44281 ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa
    44341 aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa
    44401 aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc
    44461 aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa
    44521 cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga
    44581 aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa
    44641 aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag
    44701 tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa
    44761 gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa
    44821 atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa
    44881 acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac
    44941 aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg
    45001 ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc
    45061 aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag
    45121 aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag
    45181 caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac
    45241 agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag
    45301 gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac
    45361 agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg
    45421 tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac
    45481 aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag
    45541 aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa
    45601 caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa
    45661 gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct
    45721 acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa
    45781 gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac
    45841 ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc
    45901 aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag
    45961 aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc
    46021 agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata
    46081 aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc
    46141 tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc
    46201 aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac
    46261 aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa
    46321 gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag
    46381 aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt
    46441 tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag
    46501 cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc
    46561 aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac
    46621 tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg
    46681 atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa
    46741 tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa
    46801 gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa
    46861 gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc
    46921 cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg
    46981 tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg
    47041 ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac
    47101 ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc
    47161 gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa
    47221 tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt
    47281 tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta
    47341 ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa
    47401 aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac
    47461 agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc
    47521 atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag
    47581 gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc
    47641 agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac
    47701 aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt
    47761 tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc
    47821 aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat
    47881 tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt
    47941 ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag
    48001 aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa
    48061 aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact gacccgaagc
    48121 atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag
    48181 tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat
    48241 tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt
    48301 ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc
    48361 aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag
    48421 ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt
    48481 atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc
    48541 aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta
    48601 aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat
    48661 cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca
    48721 aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt
    48781 taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac
    48841 agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg
    48901 tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt
    48961 taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca
    49021 cagatatccg agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc
    49081 aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa
    49141 ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa
    49201 tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc
    49261 cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt
    49321 ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga
    49381 atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa
    49441 tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac
    49501 ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt
    49561 accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga
    49621 acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag
    49681 agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc
    49741 ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa
    49801 aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt
    49861 ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa
    49921 atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac
    49981 tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga
    50041 cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag
    50101 agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat
    50161 ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca
    50221 tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg
    50281 atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg
    50341 gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt
    50401 atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag ctagctgaaa
    50461 aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg
    50521 aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt
    50581 tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc
    50641 ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc
    50701 ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg
    50761 catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg
    50821 gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt
    50881 gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac
    50941 tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga
    51001 ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat
    51061 ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga
    51121 aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt
    51181 ttaatgcaat ctctgtggaa ttttcgaaag atttattttt ttaatacagt ctcaatataa
    51241 ctattcaaac ataaaataac gttatttgat ttatttgttt atagtaaatt ttatttagta
    51301 atcattgctt taaaatacta cgcttatata acagagaaaa taagcgttat aataaagaac
    51361 agtattattt aaaatgacag aaaacattac tattaaagaa ataaaaaaca taaaaaatct
    51421 taagaaatcg gtttcattta gcttttgaga taaaataata gtacagtttt atacgctgtt
    51481 tcatatgttt agaaaatttg aattagattt gaaaactcta atttttaaat gataaaaaaa
    51541 gcgagaatgc tggttttact gtatattaac cttagataac tttcattttt ttaaaaaaat
    51601 tatgaaaaaa aggtagctat ttaaaagttg attatgtata atagaaacag atgggagatt
    51661 attatgatct tctgttacaa aaaagtgatt cttatcacga aaaggagtgg tgcacgttgt
    51721 taacattata tacttcccca agttgtactt cctgcagaaa ggctcgtgcg tggttgcaag
    51781 aacatcagat tccatttgtt gaacgtaata ttttttcaga accattgaac agttctgaat
    51841 taaaagcgat tttacaaatg acagaagacg gaacagaaga aatcatctcg acacgttcca
    51901 aagtattcca aaaactgaac atggatttag atgatctgcc gttacaagaa ttattggaac
    51961 ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat gattgatgat aaacgtctgc
    52021 aagttggttt taatgaagat gaaattcgtc ggttcctacc gcgcgatgta cgacaattag
    52081 aattacgtca agcacaattg atggctggat tatagaagga taatacgaaa cttggaaaag
    52141 actattacaa agatagtttt tccaagtttt tttgttttta caaaaagtat tcttgattag
    52201 atgtataaaa aggtagtaga agatgatgat tgcacgaaaa atagagaggg aaaattagct
    52261 gaaaagtcgg ctatatagta ttcgtctgcc aaaaatgtta tagttaaata agatagcttg
    52321 aaaggttgaa aaatccgctg gttttattaa attcgcttaa cagcgagatt ttttttcttt
    52381 acatttaagc tgatttcgct acaatgaatc tatattaaac attgtggcaa agtgaggtgt
    52441 agcaaaatgg aaatggaaca tattaatgaa aatacgatcc gtgtactaat tggtcatgaa
    52501 gatttagagg aacggggcgt ttcatttttg gatttacttg gcaatcacaa agaaatcgaa
    52561 aatttctttt atagtatttt ggaagaagtt gatatcgaag aggagttccg aggaagcgaa
    52621 gctgtcactt tccaagtttt gccaaaagga gacggattag aactgtttat cagtaaaaat
    52681 cttccggatg aagaaatttc tcaatcagaa gattctactg ttgattcaga agatgtgagt
    52741 gactttcttc gtcagcagat tattggcagc gatccggagg aagaattatc tggaagcgcc
    52801 ctagaagaac aggaaagaca agcaactttt gcttttgaag attttgaaca agtgatccaa
    52861 cttgcaacag atgtcgaact agagtctgct tggactgatt tgtatttatt cgaagagcgc
    52921 tattacctag tcgttcactt ctggatggaa aatctcaacc aagcggatgt agagaatcag
    52981 attgctcgaa ttttggagtt tgctgaaaag agcgatcgta caccagaaac attgaatgaa
    53041 tatggacaat gtctgatggc gcgtaatgct atcgagcgaa cgaaatttta ttttgtataa
    53101 atcagcatta tagtaagaga ctgtgacaaa aattgtgtcg cagtcttttt ttcgtataaa
    53161 tgtgttcaaa agcttgcatc tgcggtaaca agcccaacca aacgaaaaat attgggaact
    53221 atttttgttt agctgttctt attacttgat gcagaatagc tttttcacgc cctctttttt
    53281 cgtgtaaata aattcttaga cgtaaataag ttcagaggga gaaaaatgct gcttgcaatg
    53341 aatgaatata ataagtgctg ttttgctaaa catgcagaaa aaggaaagaa atattattgt
    53401 cgtggctgtc gaaaaagagt gattttgaaa aagggaaaga aaaaatgtgc tcattttgcc
    53461 catcaaaaat cagataactg ttcggtcttt agtgaaagag aatcaaaaga gcacttacag
    53521 ttaaaggaat gcttcatgga ttggctggga cagtcagtag aaccagtatt tttggaagcc
    53581 tatttgccga ggttgcggca aaggccggat attttgttgg cgaatctagc aatagaaatc
    53641 caatgttcta gactgtctca ccagagattt attgaaagaa cccaaaatta tttgaataac
    53701 agttaccaag tatggtggat acttggtaat tcttttcttg gacaatcgca attctcattg
    53761 atagaaaaaa gttgctgcta ctataataga aaaagaggag tgcattgctg gaaagctgat
    53821 ctcaagaaac aaaagcttta tttgtatcat catattacag agactgtatc agggcatatt
    53881 tccttttgca gtagctgctg gactttttct agtagggacc ttaaagaaat attcactagt
    53941 aacgaaatta aaatgaacca aatgaaaaaa atagaaaaag tgagtgaaga tgggaagaaa
    54001 tggctgacac gccaacttat tcacaagcaa aagaatacag tatccataca agaacaatgc
    54061 tacttgcgtc ataaacatct tttgcatcta tcatcgtgga tctatcaaaa aagtcggttt
    54121 ttcttttatt tacaagaaca ggtttttctt tatcgaatgt tgtacgaaga agccttggaa
    54181 aaccagaaag ttcctgattt caactcatgg ctttgccaaa taaaagaaca taaaagggaa
    54241 tggctttttc ctatgattga tgaagagatg gtctatcagc agttttttaa cgaatgcata
    54301 cacttgtctt ctttgaaaaa atagtaagta taaaaatagg ctaggaatcg gttttctatg
    54361 taaaacaaga tttagttatg ctaaaattga tggaagcaga cacatgaatg agtagaaaag
    54421 ttagatgctg tgacatgtgg atgttagact atgatctatt ttgatagata gagctaaggg
    54481 aagaaaagga gtggatttca tggaagtaaa gcagttgcca aaacgagaag aattgcctga
    54541 aaatttaact tgggacttga ccaagatctt ttcaagcgac caagagtttg atgagaaata
    54601 tttggaatta tcagaagagt taaaacaatc tgaaaaacac aaaggaacac ttgatcaagg
    54661 cgcttctcaa tttttaaatg cgattgaatt cgtattgagg gtttatcgcc aaactgaagt
    54721 catttatgta tatgcgcacc ttaaaaacga tcaagacact ggaaatacag attaccaagc
    54781 gctttatgca agagcaagca gtctgttttc gaaagttagt gaagccgttt cctggtttga
    54841 accagaaata ttgcaattgt cagatgacca gatttggcaa tatttcaaag aagaaccaaa
    54901 attggaagtc tatcgccatt atatccagca aatagtagat aatcgagccc atgtcttatc
    54961 tgctgagcag gaatctcttc ttgctggagc aggtgaaatc tttgatgctt caagtgatac
    55021 atttgctgtt ttgaataatg cagatctagt ttttccaacg attgaaggag aaaatggtga
    55081 aatagtccaa ttatctcatg gcgtgtatgg tcagttgcta gaaagcacgg atcgaagggt
    55141 gcgcgaagca gcatttaagg ggttgtacag tgtttacgaa caatttagaa atacatttgc
    55201 ttctacttta ggcacacata taaaaggaca taattttaaa gcgaaagtcc gtaattacag
    55261 ctctgccaga gaagcgtctt tgagcaataa tcatattcct gaaagtgtat acgatacttt
    55321 ggtagacgtg gtaaacaagc atttgccttt gttacatcga tacatggaat tacggaaacg
    55381 tttattagaa gtggaaaaac tgcacatgta tgatctttat acaccggtct taggggaagc
    55441 tccaattacc tttacgtacg aagaagcaaa agaaaaagct ttagaagcac tgaaaccaat
    55501 gggtgaagaa tacatggcca tcgtagaaaa agcattctct gaacgttgga tcgatgttgt
    55561 cgaaaataaa gggaaacgaa gcggtgctta ttcttcggga agctatgaca caaatccata
    55621 tattttattg aattggcatg atacgctgga tcagctattt acgcttgtcc acgaaatggg
    55681 acatagtgtt catagttatt tcactcgttc gaaccagcct tatgtgtacg gcgactactc
    55741 catcttttta gcagaaattg cttcgacaac gaatgaaaat atcctaacgg agtatttatt
    55801 ggaaacagaa aaagaccctc gtgtacgggc ttatgtactc aaccattact tggatgggtt
    55861 taaaggaaca gttttccgcc agacacaatt cgctgaattc gagcatttca tgcatacgga
    55921 agatgaaaaa ggtgtgccat tgactagtga atacctaagt gatagttatg gtaaattgaa
    55981 tgcaaaatat tatggtccag cagtcgaaga agacccagaa attaaattcg aatggtcacg
    56041 gataccgcat ttttattata attactatgt tttccaatac tcgactggct tttctgctgc
    56101 ttcagcactg gcgaagaaaa tactaaacca ggaaccagag gcactagaga actacttggc
    56161 ttacctaaaa gcaggtaaca gcgattatcc tgtggaagta atgaaaaaag caggagttga
    56221 tatgacacag gctgcatata ttgaagatgc aatgtcgatg tttgaacaac gtctaaatga
    56281 attagaagaa ttgatcgatc gtttataagg ctttctattt ttgattttct cgaatagaaa
    56341 cgaaaaaaat acgtcggctc tcttgtaagg gagtcgacgt atttttt
//
LOCUS       DACCCS010000013        54446 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.013, whole genome shotgun sequence.
ACCESSION   DACCCS010000013 DACCCS010000000
VERSION     DACCCS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.013"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..588
                     /locus_tag="IVK82_000810"
     CDS             <1..588
                     /locus_tag="IVK82_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391889.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(828..911)
                     /locus_tag="IVK82_000811"
     tRNA            complement(828..911)
                     /locus_tag="IVK82_000811"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(875..877),aa:Leu,seq:gag)
     gene            1294..2511
                     /locus_tag="IVK82_000812"
     CDS             1294..2511
                     /locus_tag="IVK82_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6391890.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2695..3531
                     /locus_tag="IVK82_000813"
     CDS             2695..3531
                     /locus_tag="IVK82_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6391891.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3607..4755
                     /gene="nagA"
                     /locus_tag="IVK82_000814"
     CDS             3607..4755
                     /gene="nagA"
                     /locus_tag="IVK82_000814"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP6391892.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4976..6034
                     /locus_tag="IVK82_000815"
     CDS             4976..6034
                     /locus_tag="IVK82_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP6391893.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6053..7072
                     /locus_tag="IVK82_000816"
     CDS             6053..7072
                     /locus_tag="IVK82_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP6391894.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7174..7890)
                     /locus_tag="IVK82_000817"
     CDS             complement(7174..7890)
                     /locus_tag="IVK82_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP6391895.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7859..8644)
                     /locus_tag="IVK82_000818"
     CDS             complement(7859..8644)
                     /locus_tag="IVK82_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6391896.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8635..9384)
                     /locus_tag="IVK82_000819"
     CDS             complement(8635..9384)
                     /locus_tag="IVK82_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP6391897.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9682..10206
                     /locus_tag="IVK82_000820"
     CDS             9682..10206
                     /locus_tag="IVK82_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391898.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10222..11721
                     /locus_tag="IVK82_000821"
     CDS             10222..11721
                     /locus_tag="IVK82_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP6391899.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11809..12444)
                     /locus_tag="IVK82_000822"
     CDS             complement(11809..12444)
                     /locus_tag="IVK82_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391900.1"
                     /translation="MIVKRPKRRISMQFDRFRLGMRTIKSALAVFLCILFFHLTQRGI
                     PMIAALSAVFSLRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILL
                     PILVIIVIVVSDGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAI
                     GINFFLRPPETEKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12515..13471
                     /locus_tag="IVK82_000823"
     CDS             12515..13471
                     /locus_tag="IVK82_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP6391901.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13776..14732)
                     /locus_tag="IVK82_000824"
     CDS             complement(13776..14732)
                     /locus_tag="IVK82_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6391902.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14906..15466
                     /locus_tag="IVK82_000825"
     CDS             14906..15466
                     /locus_tag="IVK82_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391903.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15459..16283
                     /locus_tag="IVK82_000826"
     CDS             15459..16283
                     /locus_tag="IVK82_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP6391904.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16293..16910
                     /locus_tag="IVK82_000827"
     CDS             16293..16910
                     /locus_tag="IVK82_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP6391905.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16928..17611
                     /locus_tag="IVK82_000828"
     CDS             16928..17611
                     /locus_tag="IVK82_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP6391906.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18294..19577
                     /locus_tag="IVK82_000829"
     CDS             18294..19577
                     /locus_tag="IVK82_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6391907.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19593..20480
                     /locus_tag="IVK82_000830"
     CDS             19593..20480
                     /locus_tag="IVK82_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6391908.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20497..21333
                     /locus_tag="IVK82_000831"
     CDS             20497..21333
                     /locus_tag="IVK82_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6391909.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21336..21959
                     /locus_tag="IVK82_000832"
     CDS             21336..21959
                     /locus_tag="IVK82_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391910.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21956..23248
                     /locus_tag="IVK82_000833"
     CDS             21956..23248
                     /locus_tag="IVK82_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6391911.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23607..23704
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23820..24401
                     /locus_tag="IVK82_000834"
     CDS             23820..24401
                     /locus_tag="IVK82_000834"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP6391912.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24404..25711
                     /locus_tag="IVK82_000835"
     CDS             24404..25711
                     /locus_tag="IVK82_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP6391913.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26127..26615
                     /gene="purE"
                     /locus_tag="IVK82_000836"
     CDS             26127..26615
                     /gene="purE"
                     /locus_tag="IVK82_000836"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP6391914.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26599..27732
                     /gene="purK"
                     /locus_tag="IVK82_000837"
     CDS             26599..27732
                     /gene="purK"
                     /locus_tag="IVK82_000837"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP6391915.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27745..29040
                     /gene="purB"
                     /locus_tag="IVK82_000838"
     CDS             27745..29040
                     /gene="purB"
                     /locus_tag="IVK82_000838"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP6391916.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29220..29597)
                     /locus_tag="IVK82_000839"
     CDS             complement(29220..29597)
                     /locus_tag="IVK82_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391917.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29853..30581
                     /locus_tag="IVK82_000840"
     CDS             29853..30581
                     /locus_tag="IVK82_000840"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP6391918.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30581..30835
                     /gene="purS"
                     /locus_tag="IVK82_000841"
     CDS             30581..30835
                     /gene="purS"
                     /locus_tag="IVK82_000841"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP6391919.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30837..31508
                     /gene="purQ"
                     /locus_tag="IVK82_000842"
     CDS             30837..31508
                     /gene="purQ"
                     /locus_tag="IVK82_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP6391920.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31509..33731
                     /gene="purL"
                     /locus_tag="IVK82_000843"
     CDS             31509..33731
                     /gene="purL"
                     /locus_tag="IVK82_000843"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP6391921.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33716..35155
                     /locus_tag="IVK82_000844"
     CDS             33716..35155
                     /locus_tag="IVK82_000844"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP6391922.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35187..36230
                     /locus_tag="IVK82_000845"
     CDS             35187..36230
                     /locus_tag="IVK82_000845"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP6391923.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            36227..36805
                     /gene="purN"
                     /locus_tag="IVK82_000846"
     CDS             36227..36805
                     /gene="purN"
                     /locus_tag="IVK82_000846"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP6391924.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36802..38337
                     /gene="purH"
                     /locus_tag="IVK82_000847"
     CDS             36802..38337
                     /gene="purH"
                     /locus_tag="IVK82_000847"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP6391925.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38353..39627
                     /gene="purD"
                     /locus_tag="IVK82_000848"
     CDS             38353..39627
                     /gene="purD"
                     /locus_tag="IVK82_000848"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP6391926.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39749..40663
                     /locus_tag="IVK82_000849"
     CDS             39749..40663
                     /locus_tag="IVK82_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP6391927.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40796..42361)
                     /locus_tag="IVK82_000850"
     CDS             complement(40796..42361)
                     /locus_tag="IVK82_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP6391928.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42354..43805)
                     /locus_tag="IVK82_000851"
     CDS             complement(42354..43805)
                     /locus_tag="IVK82_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6391929.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43819..44835)
                     /locus_tag="IVK82_000852"
     CDS             complement(43819..44835)
                     /locus_tag="IVK82_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6391930.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45055..46143
                     /locus_tag="IVK82_000853"
     CDS             45055..46143
                     /locus_tag="IVK82_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP6391931.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46197..47723
                     /locus_tag="IVK82_000854"
     CDS             46197..47723
                     /locus_tag="IVK82_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP6391932.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47725..48930
                     /locus_tag="IVK82_000855"
     CDS             47725..48930
                     /locus_tag="IVK82_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6391933.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49187..50164
                     /gene="guaC"
                     /locus_tag="IVK82_000856"
     CDS             49187..50164
                     /gene="guaC"
                     /locus_tag="IVK82_000856"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP6391934.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50428..51852
                     /locus_tag="IVK82_000857"
     CDS             50428..51852
                     /locus_tag="IVK82_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP6391935.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52313..53422
                     /gene="pdhA"
                     /locus_tag="IVK82_000858"
     CDS             52313..53422
                     /gene="pdhA"
                     /locus_tag="IVK82_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP6391936.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53425..54402
                     /locus_tag="IVK82_000859"
     CDS             53425..54402
                     /locus_tag="IVK82_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP6391937.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        17402 a         9315 c        11921 g        15808 t
ORIGIN      
        1 tttttagaca tgaatatctt atttttattg ctatttttct taattttcct tctaacatat
       61 aggaaatgtc aaaactatta tgtgcgtata ttgactgcat taccgttttt cttaaacctg
      121 attatttatt ttggtaatac aatgggtcaa agctttactt acgtcaatgg aaataaaagg
      181 gcaatgattt ggagtagtag taacctgaat aatctcttta cagagttagg aacgaaactg
      241 tcactttttt atccaggtac ttggatagct actttagtag tcttggcatt attactatgt
      301 ttaattgttg gaatttattt aagttttgac aataagaaaa cttctatttt tttagttatt
      361 ttgatgataa tgggattttg ttcaaggtta attatgggat tttctcccac agtatgggct
      421 tccggaatgc ggacgtacta tatactttat gttgttatcg ccatcttagt actaatggca
      481 gttaaagagc taatgaaaag catgagtgta caaaaaaatg aatttatgca atttggatta
      541 accgtgcttg gtatttgtac ctttataata acggtgataa acagataata tacaaaaaat
      601 ccctcgcttt agtattaggt gagggatttt actcgttttt cgattaattg ttttaacact
      661 tgcaagtttc tacgagtagc ttgtttccga ccttcatata ctcacctcag gctgtttaca
      721 tgattgtacg ttctttttgt aaaggataca gtgatatgta tataatggca taaagaaacg
      781 ttcaaacaaa aaaactcgtg aagcaaaatt aacttcacga gttgtagtgc agatgatggg
      841 actcgaaccc acacgagcta acgctcacat gaccctcaat catgcttgtc tgccaattcc
      901 aacacatctg cgtaataata acacactaat gatagcgagg ttttacatag ttgtcaagct
      961 caaaaatggg aggacttatt aagatggaga aatatgacgg agaattttca ggactgggaa
     1021 tgatcttagg tgtattgatt ggacttgcat tcggacgttt tttgcttggt tttatgctag
     1081 gaatcatttg tggaattgca atggattggg cagcgaatct atggaatgac tatcatgaca
     1141 attaagttct caacgcttgt ttgtgttttt ttaatactta cgtttttcct aaataagtgg
     1201 caaaattttc taggatggag tatgatattg aaatagtaaa taacaaaaaa tttggaaagt
     1261 tctaactttt tgaaatgagg gattggctaa gtgatgagag aatttgaaaa atccaacaaa
     1321 ttagaaggtg tcagttacga tgttcgcggt cccgttctag aagaagcgga gcggatgcaa
     1381 gaagagggca ttagtatttt aaaattgaac acgggaaatc ccgcaccttt tggatttgaa
     1441 gcaccaaacg aagtcattcg tgatttgatc atgaatgccc gtgactcaga aggctactca
     1501 gattccaagg ggatcttttc tgctagaaaa gcaattgagc aatattgcca actgaaacat
     1561 tttccgaatg tgacgatcaa tgatatctac accggcaacg gagtgagcga attgatcacg
     1621 atgtgtatgc aggggttgct ggacaatgga gatgaagtac tggttccaat gccggactat
     1681 ccgttgtgga cggcttctat tgcattagct ggaggcacac ctgttcatta tatttgcgat
     1741 gaagaggcag aatggtatcc tgatattgat gatatcaaat caaaaataac gagtcggaca
     1801 aaagctattg ttatcattaa tcctaataat cctactgggg cactttatcc aaaagaatta
     1861 ttagaagaaa tcgttgaagt agctcgacaa aataacctga ttatttattc cgacgaaatc
     1921 tatgatcgct tagtgatgga tggtctagaa catattccga ttgctacatt agcgcctgat
     1981 ttgttcgttg ttacgctgaa cggtctatcc aaatctcatc gtgtagcagg atttcgagta
     2041 ggctggatgg tcctaagtgg agataagtca cacgtcaaag gctatataga agggctgaat
     2101 atgttgtcct ccatgcgttt gtgttccaat gtattgtctc agcaaattat ccaaaccgcg
     2161 ctaggcggtt atcaaagtgt cgatgagctg ctcctgccag gcggaaggat ctatgaacaa
     2221 agagaatata tctataatgc aatcaatgat attcctggtc tttcagccgt aaaaccaaaa
     2281 gctgcttttt atatattccc aaaaattgat acaaagcgat tcaatatttt ggatgatgaa
     2341 aaattcgttt tggacttttt acatgaacat catattctac ttgtacatgg tggtggattt
     2401 aattggaacc agccagatca ttttcgaatc gtttatcttc ctaaaatgga tgatttgaaa
     2461 caaacagcta agaaaatgag agaattccta gcaacctatc gccaaaaatg attttaaaaa
     2521 ccaaattttt cagtaggagg gccacagaca aacacactga ggctctcttt ctagtggagt
     2581 tttcttaaat aactaaagta tattatttag ttgcaagtct tttcttgaag tttgcgactt
     2641 cctaaagtaa aatacgaata gaatgcgaat acgcatattt tggggaggat acttatggaa
     2701 acgttaatcg agtcctttgg aacgatcatt aaagagattc gaaaagaaca aaaaatgacg
     2761 caaaaaatgc tttcgcatga tatttgttca caaagtgtat taagtcggat cgaaaataat
     2821 gaggaactgc cgaatgtatt ggtgatgatg cagatttgtc aacggctagg ggtaacgatt
     2881 gatcatgtga tgaatctctc tcgaaaaact actcggacaa acgataaatt atttgaactg
     2941 ttggatagtt gtttccaaaa acgtgattat cggaagttag aacaagtact aaaatcttct
     3001 gatatttctg aaaatctgta tcaagcaact gactttcaac gctattatta ttatctcggt
     3061 gtttgcgaat tcacgttgcg tcaaaatatc tctcagtccc tttattattt gaaagaagca
     3121 ttaagctact ctagccaaaa agaaagaatc catgtttctg acacagaaat acaactgatt
     3181 agttgtatcg gaaaaatata tggtgtggca ggaaaaacgg ctgaagcacg ctactatttg
     3241 agtcgaagca tccaactgct gcatcaatca gctgatgaac gatcaaaaag tgaattttcg
     3301 caaatttttt ataattatgg aaatttctta ttccatcaaa atgaaatcga cgaagcgtta
     3361 gaacaggtga atcaaggaat ctattgggct caagagaaga atagctacta ttacctcaat
     3421 gatttatttg tgcttaaaag cttaatatac aagcgtaaag aagaaccaga aagagcagca
     3481 ttctatgaag gcttagcaca agctgtgaaa caaatttcaa aaaatttgta aatggtgtag
     3541 accattcttg acttcttctc taaataagag tactttagag gtagaaaaac aaagggagag
     3601 aatagaatga gaacttttat ctatgctgac aaattctttt tagcgtcagg tgtcaaacat
     3661 gcaggctatc ttgaaattac cgatggactt tttggtgaat acaccaaaga actacctgag
     3721 ggagaagtca acatcattga ccaaagcggc aaatggattg ctcctggttt ggtagataca
     3781 catattcacg gctatatgaa tcatgatgta atggataatg atgcagaagg catcaaagtg
     3841 atgtctgaag gtctgttatc ttgtggagtg acttcatttt taccaacaac attgacgtca
     3901 agcaaagaac gtttaaaaga tgtagctgag acaatcggca aaatgtatca ggaagcacca
     3961 ggagctaaaa tcaaaggaat ctattttgaa ggtcctttct tcacagaaga acacaaaggg
     4021 gcacaaaacc caagttattt cggggatcct gatttggata ctttccatga atggcaagaa
     4081 gcttcaggtg gtttaatcaa aaaaattgct ttagcaccgg aacgtcaagg tgtagaagag
     4141 tttgtcaaac aagtgactga agaaggagta gttgtttctc taggacacag taatgctaca
     4201 ctagaagaag cgcaaaaagc tgttgaagca ggcgcaagtg tatttgtcca tgctttcaat
     4261 ggtatgcgtg gattgaatca tcgcgaacca ggtatggtgg gtgcgttatt atctttagaa
     4321 catgtctttt ctgaattgat ttgtgatggc catcatgtac atccaaatgc tgcgggaatc
     4381 ctaatggaaa aagcaggaca tgatcatgtc gcattaatta cagactgtat gatggccggt
     4441 gggatgcctg atggaaatta caatttaggc gaatttccag tagtagtaaa agaaggaaca
     4501 gcacgtctag aatctggcaa cttagctgga agtatattga aattgaaaga agcaattaaa
     4561 aatgttgtag actggggatt agcaacacca gaacaagcag tcatgatggc tagtctgatt
     4621 ccagcaatca gctgtaaaat cgatgataca tgtggaaaaa tcgctcaagg acgcgatgca
     4681 gactttatcg tattgaaccc tgatatgaca ttggaagcaa cttatctaga tggcgtggaa
     4741 cgtttccacg cataatataa tttaagttaa ccttaaaact aaacttctta agtttggttt
     4801 taaggttttt tttgtgttct agtaaaaatc actttacata agtttcaaaa gtttctttaa
     4861 tttcctattg ttcaataagt aacaatgtaa agataaaatt aaaaatgtta atacgtatta
     4921 gcaaaataaa agcttttttt attgtgaata aatgaacata attaggagga gaaagatgaa
     4981 cttatttgac atttttcgat cgactttgac agatatgtcg attgtcagtg caatcacttc
     5041 aacagttttt attatattat tagggtttgt ttgtagaaaa aaggggattt tttctgaatc
     5101 tgttggaaaa attctatcga aagtcgtctt aacagtggct ttgccagcac tggcatttcg
     5161 agctttcatg caagatatca atacagaatt actgatgcaa ggaatcaatg tcttgatttg
     5221 gggattagtt atttatatta ttcttattct agtttcaaaa ccttttttct taagctataa
     5281 aggggataag caaacaacgt tgcgtgtcct gacgatcttt ggttctacga cctttttcgg
     5341 aacgcctatc gtcagtgcga tttatggtcc tgtcggcgtg atgtattcct caattttcaa
     5401 tattggctac cgaatctttt tgtattctta tggatacata aaaatgagtg gcttgaagat
     5461 ggaagctaaa aatatcaaag cgatgttcgt caatccaatc attatcgcta cttttgccgg
     5521 ccttttcatt tggctgttcc aagggtatct gccacaagtg tcagttacag gtgcagaagg
     5581 agagataagc caagtagcgt ttttgcgaat tgaccagact gcaccgtggc tatttcagcc
     5641 gatggattat ttagctggtt taagttcacc attggcgtgg ctagcaatcg gcgcaacatt
     5701 aggtgaagta agcttcaaaa aagctgcatc tgaaaaaact tcatggtatt acagtctagt
     5761 caaagtggta tttgtacctg cattgaatat cgtgattcta gcagttttga cggcaacgaa
     5821 tattctacca gtcaattttg aagcactggc aacaatcgtt atcatgatgg ctacaccagc
     5881 tgcaacagtg gcagcagcct atgctatcag ttttgatagg gaggcacttt tagcctccaa
     5941 tgcatcctta ctgtcaacta tttttgctgt ggtactgaca cctgtatgga tcgttgtatt
     6001 acaaatgatt catggcatag gtttattttc ataagaaatg gaggaagaaa gtatgagaat
     6061 cgcatgttac ggtgttcgtc cgaacgagat agattttttt gaaaaattga atatttacca
     6121 ttatgatctc agtctttatg aagaactgtt gacacatgac aatatcgtgt cagcaaagga
     6181 ccatgatgct gtattgttga ggggaaattg tatagcagat gagaccaatc ttgcaaaaat
     6241 gcaggaatat gggattcgtt atgtttttac acggactgtg ggagtcaatc atatcgatct
     6301 acaagcggca gctgattttg gtatgacagt agcccgtgta ccatcgtatt cgccaaatgc
     6361 gatagcagag ttatctttga cttttgccat gatgcttttg agaaatactg cgtatacgac
     6421 gatccgaaca tctttcaaag attttcgagt ggatgagcaa atgtttagtc gtgaaatcag
     6481 aaattgcaca gtagggatca taggaacagg caggataggg ttgacggaag ctgttttgtt
     6541 taagggacta ggagccaaag tgatcggcta cgatattcat cagacagaag ctgctaaaaa
     6601 agtattgacg ttccactcgc ttgaagagtt attagccaaa agcgatattg tcagtattca
     6661 tgtacctcat atcccaggtg aaaacgatca aatgatcaat gaaggatttt tagctcaaat
     6721 gaaaaaaggt agcatcttga ttaatacagc ccgtggagag cttcaagata atgaagctat
     6781 tttaagagct ttgaaaagca atcatttagc tggttttgca accgatgttt ttgcaaatga
     6841 aacagaggtt tttttccgat ccttcaagcc atgggagacg attccagacc ctgctatcca
     6901 gcaattagta gaaatgtacc ctagagttct tgtaaccccg catgtaggtt caaatacaga
     6961 tgaggcatta tcaaatatga tttcgacaag tttcgagaac tttcgtgaaa taatagaaac
     7021 tggaaaaacg aaaaatgagg tatctcttcc taaagcccgt caattaagat aagaaaacat
     7081 ggatattcgt caataaaatg tttaaaaata gtccaagtaa acagttgtta cttctgtata
     7141 cttggactat tttgtatggg ataattcgat gactcaacag taatctgtga gctttttccg
     7201 cccattcttt gtcaattcat aacaggttag cggccggccg atttccaaat atttgattct
     7261 ttcgttcaat atatcttgtt ctactaaaaa attcaaatat ttcttgacgg atatacgtga
     7321 cagtgagctt ttttgagcca gttcttttgt tgaaaaaata gtatcttctt ttagaataaa
     7381 tgcacttatt ttttttaaag tcagttttga cagaccttta ggcaaatcga tgaatgatgg
     7441 tatttcgtga atgggagtgg tgtggttgaa tacttgatcg atcaatttct gatcggcttt
     7501 ttcagtcgtt tggattgtct gagagaaagc aagatacttt tcgatcgcca gctgaaaacg
     7561 ttgcaaagtg aacggtttga tcaaataatc taaggcaccg tagcggatca cattctttat
     7621 ggtttcgata tcatctgcag cagtgatcaa aatcactggg atatcatttc cttgctgtct
     7681 aagccaagat aaaaaatgga taccactttc gccaggcatg tatacatcaa gcagcagcag
     7741 atctgcgttt ttttgtctga aatatttttt tccttcttct acatttctga aactttcgat
     7801 tacgttcata ttagggatgc tttccacaaa agtacgattc aattctgcaa ccattggatc
     7861 atcttcgatt atcaggacat ttgtcatcat tttgatcttc cttttttggt aattctagat
     7921 agacgattgt accgccattt gtttgtttgg aaaattctaa aaatccaccg tggcgtttga
     7981 cgattgaatg gatgagattc aatccaaacc cacgattgat tcccttcgtc gaaaaaccta
     8041 gcgtaaatat tttttctgag atttcttctg atatcccgac accgtcatcg cagacggtca
     8101 cgatgaatac ttcagcttct gtgtcatagt ttaaatgaat ccggacctgt ccgtttcttt
     8161 catgtttttg tagcacggct tcttttgcat tatcaagtaa gttccctaac acttgcaaaa
     8221 gtgaatggat ggagtcatcc atctccattt tagggataaa agaatcgctt tctaacagca
     8281 tttcaacaga ttgttctttg gcttcgttta ttttgcctaa taaaaaaccg gcaattgcag
     8341 gtactttgat cagacttgag acttggccaa tctctttttt ataattttga tttagctgct
     8401 ggataaaatg ggtgacttct tcatacttct tcaaatcaat caaaccagaa atgacatgaa
     8461 gtttattcat gaattcatgg gtttgagctc tcatggagtt tatatattgt ttcgtccccc
     8521 caagctcctc cattaatttc attacttctg atcgatttcg taaagttgcg actgctccta
     8581 aaaaacggtc attgctaaaa agaggtgtaa cgctagaaat agtttctatc ccatttaaat
     8641 acatggattg agtccgaacg gtcgtttgat gctcaatcgt ctctttaaac aaaacgtcat
     8701 atagattatc actgattttt ttcctacttc gaaatccaat agctgctctg tttggtagaa
     8761 aatgtttttt gcaggtgtat tgatcaagag gatagtttta tcagaagaga tggctaagat
     8821 tccttcatct acttcatttt cgataatcgt tttttcactt aactgttctg ctatttcttg
     8881 tggttcaagt ccaagcagta cttttttcat tttttgggca agaaaaacgg cgcctatgat
     8941 gcctacgcta agtccaataa tcaatgaaaa aacaatcatt ttttgtccat ctttgacttg
     9001 attgtcaatc gtgttgtttg ttaagccaac acaaactgca ccaataatct cttgatcata
     9061 ataaataggg acgaaatagc ggaagccgaa ccctaacatt ccttcttttt cagagaaaaa
     9121 ttcttgtcct ccaatcactt ttttcacatc ttcatgactt gagaaaggaa gaccgatgta
     9181 acttgggtct gcgtgagaat atcgaatgcc ttcagggtcc atgacaacga taaaatctaa
     9241 attcgatgca atcatgattt catttgttat tccttgaatc gcatgtgtcg ggtcaccagt
     9301 ctgcagcttt ttttggatga gtttatcatt ggcaacgatt ttagcaatgc tagagatttt
     9361 atctgacaca ctagatcggt tcatcgaaag aataaaattc ctgatcaata tggcagaaac
     9421 aatcaaagaa acgaatgtgg agaaacaaac gacaaggatg atcgtgttct ggagctttaa
     9481 tggttttttc ttaaaaaaag gaaacatagg ctacctcgaa agaatttatt gtgaaaattt
     9541 taacatgaaa acaaaaattt aacaaattgt caacgataaa cacaaccttc ctataaaaaa
     9601 caccactgag tcggtaaaag aagctataca gaattttcaa acattggtat gatagacgta
     9661 gaaatggaag gaggcgtgta tatgataggg ggaatcgcac ttgagacgat ttattggtat
     9721 acattgctga tttgtgcagg attggcagtc ctattgatta tttttggaga tgtattcgat
     9781 tttgatggcc cgcttgatcc gatgcttatt atcccttggt taactttcac aagcttattt
     9841 ggttttctag gtgagcgttt aacagatcaa tcatctttat ttttatttat tgctagtgga
     9901 ggcatatcga tagtgttggt atttttgctt aatttctata tattgatgcc gatgaaacat
     9961 gccgaatcaa cgatgtcgtc ttctgaaaaa actctggaag gacaagtagc tgttgtgatc
    10021 acaccgatcc ctgtacgagg tatgggagaa attcagttga aaagtgttac cggttccatt
    10081 agtcgtccag cttgttttta cagcccccaa gaacagacag ctgagcgggg aacgaaagta
    10141 ttgatcattg agatcaagga aagaatctgt tatgtcatgc cttatgaaag catgataaaa
    10201 gcttaaagga gcgtgtgtta aatggaaata ccagttgttt ttatcgcgat cgcaatcgca
    10261 gctttgattt tactgatgtt gctgatcgtc tttgtagcga agtaccagac agcaaaaccg
    10321 gatgaagcgt tgatcatcag tggaagttat ttaggaagca aaaatgtgca cattgatgaa
    10381 agcaataaca aaatcaaaat tgtgcgtgga ggtggcgcat tcgtcttgcc agtttttcaa
    10441 cgttcgaatc ggatcagtct attatcgagt aaattggacg tctccacccc tgaagtgtat
    10501 acggaacaag gcgtaccggt gatgtgtgat ggtacttcga tcatcaaaat cggttcatct
    10561 gtagaagaga ttgctacagc agcagaacag tttttaggca agacaagaga agaactggaa
    10621 aatgaagcaa gagaagtatt agaaggacac ttgcgctcga ttcttggttc tatgactgta
    10681 gaagaaatct atcaaaatcg cgataagttc agtcaaagcg tacaagaagt cgcttctgta
    10741 gaccttgcaa aaatggggtt gatcatcgtt tctttcacta tcaaagaggt cagagacaaa
    10801 aacggttatc ttgactcttt agggaaacca agaatcgccc aagtcaaaag agatgcagat
    10861 atagcagaag cggaagctct aaaagaaacg cgtatcaaaa aagctcaagc agaaaaagaa
    10921 tcacaagcag ctgaattaca gcgacaaaca gagatcgcag aatcattaaa agaaaaagaa
    10981 ctgaaattag caacctacaa acaagaacaa gatgttgcta aagcaaaagc agaccaagcc
    11041 tacaatttag aaagtgcccg agcacaacaa caagtaatcg aacaggaaat gcagattaaa
    11101 atcgtagaac gtcaaaaaca aatcgaactg gaagaaaaag aaatcacacg tcgtgaaaag
    11161 caatatgatt cggaagtgaa gaaaaaagca gatgccgatc ggtacgctaa agaacaagaa
    11221 gcacaagctc aaaaagtcaa agaagtgact gaagccgaag cagaacgatt ccgtgtggaa
    11281 gcactggcag aagcggaagc caataagaca cgtttagctg gacaagcaga agccgaagca
    11341 gcactagcaa aaggaaaagc agaagccgaa gcaaaacaaa agatcgccaa tgccttcaag
    11401 gaatatggcg aagcagctgt cttgagtatg gttatcgata tgctaccgca attgatgcgc
    11461 gaagcagcac agccgctagg aaatatcgaa aaaatctctg ttgtcgatac aggaagcagt
    11521 tcaggagaaa ccagtggagc gaaccgagtg acaaattatg ccacaaattt gctttcttct
    11581 actcaagaaa cactaaaagc gacaacagga cttgacttga aggaaatcgt agagaatttg
    11641 tcgacacgtg ggactactag ccaagtaaat atgtatgaag cgccaaaaga ggcagaacaa
    11701 aactcgatga aggaagaata aagtagtaca cttcaccgtt caatcaaaaa tggaggaaga
    11761 ctttttaaag ttcttctctc cattttttta ttttattgca aaagaaaatt agtcctcgct
    11821 atttttcttt tgtttttgga tcttagcttc tatctcttgt aaatttgttc ttagcaatcg
    11881 ttctttcttt tggagttcga ccaaatcttc tgatagttct tccttctttt ctgtttcagg
    11941 aggacgaaga aagaaattga taccgattgc aatgaatgtc cctataaaag tatctagtac
    12001 acgttggata gcatacaaag cggattcgcc ttgagggatg ctcaacgcaa tcagcaacaa
    12061 cgtagctatt gcggaaatga taccggaatt attgttaata ccatctgaca cgacgatcac
    12121 aataataact agaataggaa gcaagatcaa ttctaccaaa aaatcattgt gaaaatagct
    12181 tttgacaaaa aaataaatga tagctaatgt accacctaat gtattgccga tgatcctaga
    12241 ctttccaaaa gaaacggttg tagtcaagtc ctgacgcaaa gaaaaaacag cagataatgc
    12301 agcaatcatc gggattcctc gttgagtcaa atgaaaaaac aagatacata aaaaaacagc
    12361 taaggcactt ttgatcgtac gcatacctag tcgaaaacgg tcaaattgca tagagattct
    12421 cctttttggt ctttttacta tcatacctaa acttttgtct gatagctagt ttttcattat
    12481 tcaagtataa ttaaaataaa aaagggggaa gtaaatgcgt ataggaatgc gaacagtcaa
    12541 aacagtgatc agtgtcttct tttcgatggt cacagcgggt agcttatcgc tgttgtattg
    12601 gccagctgca gggatcattg cattattaag tgtaggaaat accaagcgct ctacattgcg
    12661 tacgggcatg aatcgaatcg cagcctttat attagctacg gcaacagcac tgttcagttt
    12721 ttttgctgtt gggtatacta tttttggctt cagtcttttt ttattcctgt tcatcccgct
    12781 tgcctctcgc ttcaaattaa ccgaagggat agtagtaaat tcggttttgg tcactcatta
    12841 tttagtggag gaaaacatgt cttggcagct gatcggcaat gaagcgggat taatggcgat
    12901 tggattagta tttgctttgc ttgcaaatgt ttacatgcca gatcgtacga agcagctgaa
    12961 ggaaaaccaa atacagatcg aaaaagaatt tcgaaatatt ttgagacaaa tggcagaatt
    13021 tcttttatca gaaaataaag aagatgtgca gatcaaatgc gaacatttac tgacattcat
    13081 tcgcgaaagc caagaagatg ccagagaaca tcaagaaaac tactggctca gacagccgct
    13141 atactacgaa acttacttct ctatgagaag agcacaggcc aatgtgatta aagacatgct
    13201 ggaaaatcta gagagaatcc agcaaccagc ttattatgga aaacatatct atggtttatt
    13261 gatttatact gcagaaacat tttcagagtc aaatgatggc agacaaattt taacacgtat
    13321 tgaagaagtt tatgtattgt atcggcaaat gcctctgccg actagtcgcc ctgaattcga
    13381 ggatcgagca gaattgttcc agtttttgca gtcttttaaa agttttattg aaatcaaggc
    13441 agaattctcc caacaaaaaa tccaaaaata gaggttgtga attcagctaa tattcaggtc
    13501 aaaaagctga tttcacaacc tcttctttaa acggtgttct cctgtcaact cctcggtcac
    13561 aagtcacaat attcaacaaa catgagaagc tgttttttga aattgctcct tgtgactcgg
    13621 agctgaacgg taggctcaca acctctttct taaacggtgt tcaaaagctt tcctctgcgg
    13681 taataagtcc agccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac
    13741 ttgatgcaga acagcttttt cacaaccttt taaaattaca tatttttcca tagaacttgc
    13801 atttgatcca agatttcgat gacatctttt gtcaagaaga gagagcgatt gggcaagtca
    13861 ccttcaatca tgtgtaccat attctttatt tcgtaattca ttgcttcaga tgtattcccg
    13921 ctttcgatga attcctttgt accatctgta aataagatct ccgcacgatc agcacgcggg
    13981 taatcagcaa tcgtcacata accgttctca aaggcaacga taccgacttt tggcattttt
    14041 gcctgaaaag tcaagctgac agcagcaagt tcattttctt tgttacgcaa gatagtgacg
    14101 gattgttcgt ccacgcctgt ttcaaaagga accattgtag aggcaactac ttcaggttga
    14161 gagctcaaaa agaaacgagc gaaagataca gcatacgtac cgatatccaa cagagctcct
    14221 ccagctaatt ctggattgaa gaaccggttt ttagggtctg gttctttgta actgccgaaa
    14281 ggtgcttgga tcatttttaa agcccccagt tttcctgtat ccattattga gcgtaactgc
    14341 tgataaagtg gcatgttgaa gattgtcatc gcttctgcta agatcaaatt cttttcttct
    14401 gctaagcgca tagcgtcagc cagttctttt ttattcatcg tgatggcttt ttcacataga
    14461 acgtgtttgc ctgcttccaa tgctgccaag atatggtcga tatgctgtct atttggtaca
    14521 gcaatgtaca cgatgtcgat ttcagcatcg tctaacattt cttgatagct tccgtaagct
    14581 tttggaatat tgtaacggtg agcgaagttt tccgcttttt ctgaagttct ggaagcaact
    14641 gctgttagtt cgctcgtctc ttgattgaag ctttcggcaa actcatgtgc gatggtacca
    14701 agaccaacga ttccccattg atattttttc atctgaaaac gctccttcat agttggattg
    14761 agtttcttgt ctgtctatta ttataacaaa aatcacgaag aataaagagg agaggacttg
    14821 aaacattaaa aaaaatgaaa gcagcgattt aagaggtgct ttttgatcaa aacttaagta
    14881 gaatagttaa aaggagggga taaacgtgca tcaagtatat attgatctta ttatgagtgt
    14941 attattagaa cagtttgaaa ctgaaaaaaa ttttttagat gaatacttgc tgatcagtcc
    15001 agaacaatgg caaaaatgga aagatggcga gaaaaatctg ccatctgaag cgatgcagaa
    15061 agtaaaaaat cttttcactg attatgaatg gatgctaacg caaaagattt tgcgtcaaac
    15121 gattattttt cccgaaaaac gaaacgttgc cgttgcagag tacaagcaaa tcaaaacacg
    15181 catcgcgcaa aaatggctta gcagcaaagc agcaaaagtc gagctgatcc ctttgaaaaa
    15241 taatcagcag gctggtagat accttgactt gaaagtaagt attcaatatg atgaatgggg
    15301 ctatgatgat attttgaatt ttcgtctgcc agcagatgtt caaaaacaga tcgaaggaga
    15361 aaaaatcgaa cttctcgatt gggtgaatga aaatctggaa gaaacttacg ttcatgaggc
    15421 gaatggggaa cgtgaagaac aacgaaagga tttttcaagt gaataaattt ttaggatttg
    15481 gagcagttgt gattatgatc gcagcaatgg gctatgttat ttttagcgat gatgattcgc
    15541 atgttcaagc cggcaatcaa gcagtcgaac gaacaagcac caaaacaact gctagtaaag
    15601 aaaagaacag tcaaacaacc agcagcaagg taaaggacaa agaaacaaaa gatcgtacgt
    15661 ccaaacaatt gccagatgtt aaaacagacg attggaagct agttttggtt ggtccagatc
    15721 acaaattaga aaaagaaatt gatgagtcta ctcaattggc atcattaagt aacggatact
    15781 tgatcgacaa acgcattgct ccagaatatg aagcatttgc caaggctgct gaagcagctg
    15841 gatttccatt agtgatggtc tcagcttacc ggtctgtatc atcccaacaa caagttttcg
    15901 aacaaaatgt ccaagatgtg atgagtcgtc aaggtgtatc agaagaggag gcgacaaaga
    15961 ttactaaaga aacgattacg gagccaggat acagcgaaca ccacacaggt ctggcagtcg
    16021 atgtcgtcga tcaaaactgg tacaacagtt atccttctac tgtattggat gcaagttacg
    16081 gagatcagcc tggagctaaa tggatcgcag ataatgctgc gcagtatgga tttatcgttc
    16141 gttatccaaa aggacacgaa gatataacta agatcacata tgaaccttgg cacttacgtt
    16201 atgtcggtaa agaaaacgct gagtatatca ccaaacacca attgacgcta gaagaatatc
    16261 tgaatgaatt aaatgaaaag taggaattga tcatggcgaa aaaaaagtgg aaaaagcaaa
    16321 agcgcaagaa tatccgttgg ccagcagtgg ttgccggtgt agcagtgata ctcttagcct
    16381 ttgttttttc cttgaagaat ctgtcttctc cttttactga taatcagcaa gaaaatgaag
    16441 cgcaatccca tcagcaattt attgaacggc ttgttccgca tgcccaagaa ctacaagatg
    16501 gttatggagt gcttcctagt atcattttag gacaagctat attagaatca aattggggaa
    16561 aaagtacctt ggctagcaaa tacaataatc ttttcgggat caaggcttat ggggatcaga
    16621 agaaagtaag cctggaaaca aaggaatttg ttaacgaaga atggataact atccaaggag
    16681 acttcaaagt gtatgattca tgggaacagt ccatggatga ccatacacaa ctgttcgtcc
    16741 aaggagtaga ttggaatcct gcgctgtatg aaaaagtgat tacggccacc aactatcagg
    16801 aagccgcaca agcattgcaa gatgcaggtt atgcgacaga ccccggttat gcgcaaaaaa
    16861 tcatccaggt catcgagacg tatcaattga atcagtatga ccggagatag caaaggagaa
    16921 gtggctgatg gcagaacaat ttacgccaga aatcatgacg gaattgagaa aagatatcgt
    16981 aagagtaccg gaaatcattt tagaagcaag cgggatcaag atatttggaa aattgatccg
    17041 ttcgatcatc tttactacgg atattgcgat catacgtaat acaaatgcga atgcagtaat
    17101 tgctgtgtat ccttttacac ctcatccagc gattactaaa agcattattg aggcagctga
    17161 cattccagtt ttctccggtg taggtggtgg tttgacacaa ggtgctcgtt cttcctacat
    17221 gagcatgttc gcagaagcgc aaggttcgtt aggggtcgtt ttgaacggtc ctacgccttt
    17281 agagacagta aaagaagttt gtcgagtagt tgatattcca gttatcagta cggtaacgtc
    17341 gaaatataca gaaatagatg aaaagttagc agctggtatt tcgattatca atatcagtgc
    17401 aggtaaagaa actgcagaag cagtacgcca ttttcgtaaa aaatatcctg aacttcctat
    17461 cattgcgaca ggtggtccaa cggaagaaag tattgctgag accattcaag caggagcaaa
    17521 tgccatcaca tatacgccac caagtagcgg agagcttttt agtcgtaaaa tggaaaaata
    17581 tcgggaaact gagaaaaaga atcatgaata acaggtgaga atcaaaagac gaggaagagc
    17641 agatagtggc tcttcctcgt cttttgtact ccagtactat ttgttcagaa ttcaaaaaaa
    17701 agagaaattt ttttgttttt ttacctgtaa tgtacataag aagagttaaa accttttgtt
    17761 attattagca ttctaacaaa aaaatgtctt gatttattga agcgctttct atagaatgaa
    17821 aaacgaataa aaactaaaaa aggcacccga caatttagta gcagctaaat tgtcggggct
    17881 tgaaaagtca gcgggaactg accaatcaag ttgccatagt caatgcttgt gcatcgacgt
    17941 gtatattgta cctaatttca tacagaatta ctagttattt ttctgttttt tagggaagag
    18001 cgcacacgaa gaagcatgct tatttagcta tgtcaaggat attgggcagt tgtctgatat
    18061 ccttttttct ttgcgtaatt atcctcccat cattgaagca agaagtgttt ttagcgatga
    18121 aagaagaatc agcactatct aatttttctt gaaagtattg agttgttcat cctaggcaga
    18181 acgataaggc aagtcttggt ttttattccc cttgaaaaag atcgtttaat aaatatgttc
    18241 tgtataaaaa tacagtatat tgaaaaataa caatggaagt agaggtgcag agaatgaaga
    18301 aaagaatgag aatcatctta gctttaagtg ttatgttatt cttcttgacg gcttgtgggc
    18361 aagaagatga caaaaaaatc acgctgaaca tggcttggtg gggttcgcaa gtccgacacg
    18421 atgcaacagt aaaagtaatc gagctttatg aagaaaaaaa tccccatgtg acgatcgatt
    18481 atgaatttta tgattatgaa ggatattaca caaaattagg tacactagca gcatcagatg
    18541 atatctggga cattttccaa ctgaacaatc aatttcctca atacatcaat cagattgaac
    18601 cattagatga atatgtggag caagggataa ttgatatttc taatgcagat gaatcctatt
    18661 tagcaacgac gacttacgac gggcaattag ttggactgtc taacggggtg aattcgtttg
    18721 cgattgctta caataaaaat atatttgaac aattaaatat ccctgaacca gcttcaaatt
    18781 ggacatggga tgaatttgaa caaatcagct atcaaatcac tgaagaatta ggaatctatg
    18841 ctatttcaag attcgaagat tttatagctg gctgtattat tcaaatacca caagttgaga
    18901 aaggattgaa tttttataat cgggaagatc tatctaacag cttgggattt gaaaatccag
    18961 actatctaac agattttttc cagctgcgta aaaatctggt agacaaaggg gcccatccag
    19021 aaccaggtgg tggagctgca agtacgacgg atctgtcttt tcaggcggtt agggatagag
    19081 aagcggcaat ggtgtttcta tcaagtaacc agttgacaga aatattgtca ggggctcctg
    19141 aagatatgga gattcagttg attaatccac ctcgcagaaa agcagatgga gaatctggta
    19201 tccctttacg ttcttcgcaa atgctgagta tggcaaaaag ctcagcccat aaagaagaag
    19261 ctgctaagtt tattgatttc tttcaaaaca gtgaagaagc caatgaaatt ttaaaaggag
    19321 aacgtggtgt accaatcatg tcgaatattc gagaacagct cgctcgtgca gatgatgcaa
    19381 ctttgcaagc gacatttgat tatttagaaa tgatttccga tatggacaac ggcgaagtga
    19441 atgtctttga tagtgctttg aatccggaaa ttcaagacca atacaatctt ttagttgaaa
    19501 aagtcattta tgaagaagta acagcagaag aagcagcaga gagtctttat gagtttgcgt
    19561 caaacattct aggatagcag caggaggaag gcatgaaaaa agaaaaacta aaacggttct
    19621 tggatcaaga caatgtggtc ggttacgtat ttgcttctcc attcatccta ggatttctat
    19681 tgataacagt gataccgatg atcttgtcac tttattattc gttcaccgat tatcgtttgg
    19741 gaaatccagt ctcatggatc ggcttggata attatataaa gatgctgagt gatgcccgtt
    19801 ttttgaactc catccgagtc acttttctct atgtatttgc atctgtacct gtgaaactga
    19861 tctttggtct attgattgct tatcttttaa cgaggaatat caaaggaacg actttctttc
    19921 gttctttgta ttatgtgcct agtttgatcg gtggcagtat ttctatcgct ctagtttggc
    19981 gggagttgtt ttctaaaaac gggttagtga atattttcct ggaacgatta ggcgcacctg
    20041 cgctttcctg gtttagcgat caaaaactgg tcatgatccc tcttgttttg atgtctgctt
    20101 ggcaattcgg ttcggcaatg atcatctttg ctgcaggtat caaagagatc ccaggctcgt
    20161 attatgaagc ggcagagatc gatggagcga acaaatggca gacgttttac aagatcacgt
    20221 tacctcttct ttcgccgatc attttgtata acttggttat gcagacaatc gcggcgttta
    20281 tgacttttac gcaggcattt atcattaccc aaggtggtcc gaatgatgcc acgaactttt
    20341 atgctcttta tgtgtataac caagcttttt cttttaattc tatggggtat gctagtgcta
    20401 tttcgtggat catgctgttg attatgggag ctgtcacctt tctgattttc aaatcatcaa
    20461 aaaaatgggt cgttgtgtga atgagaggag gaagaaatga ataatacagt aacgaaatgg
    20521 tttcaatacg gttttatttt gctattaggt ctagtcatga tttatccggt cttatggatg
    20581 atcgcaggtt cttttaaaac ggatcaagag attatgagtg gttcgctgaa tttgattcca
    20641 gagacttttc aatgggaaaa ttacgcaaaa ggatgggcgg ggtttgctgg aatttcattt
    20701 tttactttct ttaggaattc gtttgtcatc tctgcagttt cgacgatagg gacagttctg
    20761 agctcgactt gtattgctta cgcattatcg agaatcgatt tcaaaggaaa aagattctgg
    20821 ttcgtcgtca tgttagtaac gatgatgatt cctgcacaag tgattttgat tccgcagttt
    20881 attatttaca atcggctgaa tttggttggg acgtatgtgc cgctgatttt accacatttt
    20941 tttggacaag catttttcat ttaccagatc atgcaattca tggtcagcat ccccaaagag
    21001 ttagatgaat cagcgattat tgacggatgc agcaagtata gtgtctttac caaaattatt
    21061 tttcctttgc tgaaaccttc gatcatcacg acgatcatca tccagtttta ttggaaatgg
    21121 gacgacttca tgggaccatt gatttattta aacaagccga gatcttacac ggtatcgatt
    21181 gccatcaaat tatttgcaga cgctggatct agtacgactt acgcgagtat gtttgcgatg
    21241 tctacgttgt cgctgctacc ggtttttctg attttcttat tcttcaataa atatcttgtc
    21301 caggggatca gtacaagcgg tctgaagggc tgaatatgaa aaagccaata gaagagatgt
    21361 ttctttacaa gctcaactac tatgtctgtc ggattttttt aaccagtctt atcagtaatg
    21421 ctcttttcgc tttagttcta ggatgtttgt tcactttagg attagtgctg tctgctcgtg
    21481 ttgtctatct gatttatttt ttgatgatgt atccttctta ttatctatct gtccattact
    21541 tggcagaaaa aaaggtatgt tggaggaaag gaaaaatcaa acggatgtgt ttcatcatta
    21601 cgttgtttca gctgtttgtc attatttttg cagttgattg gtatttatta gtgggaaaga
    21661 ttggagcttg gtatctgatt ccttttctat tgtgtctgtt gctttttgta tcccatagtt
    21721 tttttagtac gctaattgat atctgcaaag aagataaagc agatagtctt gtttatttga
    21781 tcaaaaggaa tatctggctg ttgtcaaaaa acgtcaaggg gtttctgtgg tatttatgcc
    21841 ttttgtatgg actgttctgg tgtatacagc atgatttttt actcactgca ttactgtatc
    21901 cagggctgcc atttgtcaca gtattttgct taaaagaaaa ggagaatgac tttaaatgaa
    21961 gaaaaaatat gtacaagtcg gacttggtgg gagagcgcgt tttttttatg aggcaattgc
    22021 ctctacgtat catcaaactt gtgaactagt cggtttttgt gatgtatctc aaacaaggat
    22081 ggattatgca aatcggatac ttgtgaacaa gtatggacag aaaaaagtaa agacatgtat
    22141 gccatcacaa tttgagcaac tactggatga cgtacggccg gctgaagtca ttattacgtc
    22201 agtagatcga acgcatcatg attatatcat tcgtgcaatg aaaaaaggat atgatgttat
    22261 ttgcgaaaaa cctctgacta tcgatgagga aaaagctcaa gaaataataa cggtccaaaa
    22321 acagacggga agaagcctgc gagtcacttt taattatcgt tatgcaccgt atcacacgaa
    22381 gataaaggaa ctgatagaaa gtgatgtgat tggtgatgtg ttttctatcc acttcgaatg
    22441 gctgttaaat acgcaacacg gggcagatta tttcagaaga tggcatcgaa ataagcacaa
    22501 tagcggcggg cttcttgttc ataaatctac ccatcatttt gatctagtga atttttggtt
    22561 gaacagccgt ccccaaaagg tatttgcatt cggtggatta aacttttatg ggatgcataa
    22621 tgcggagaaa cgcggagtag aaatatttta cagtcgatgc tatggaagcg aactagctaa
    22681 agctgatcca tttgctatag acctgtcagc caatgaggaa ttgcgagaaa tgtatttaga
    22741 agcagaaaaa gacagtggat atattcgtga ccgcagtgta ttttccgatg atatctctat
    22801 tgaagataca ctaggcgtaa cggtcaaata tgaaactggt gcgattttga cgtattcgct
    22861 aaacgcgtat atgccgtggg aaggattcaa tgtttctttc aatggcagca aaggacggat
    22921 cgaaatgaaa gtgatcgaaa agtcttacat caatgcaggt ggagataaaa aggatgaagg
    22981 agcagccgtt cttcagtcca tccatgtcta tccgatgttc ggtgagccat atgaggtaat
    23041 cgtcgaacaa gaagcgggcg gacatggcgg aggagatccg cgtttattag atgatctatt
    23101 tggtgaagaa aaagaagaag atccgtggaa tcgggcagca actcacgtag atggtatttt
    23161 gtcgattttg acaggaatcg cagcaaatca ttctattgct tcaggaaaag cagtagtgat
    23221 tgacgagctt gtcagcttaa aagaataaga atagatttac ggaaattact tcccgtagtt
    23281 tataaaatct tgccttattg ccgataggct gtcttttgaa cactatttat tcggaaaaag
    23341 gggctatgac atgctttttg tcatgccttc tttttatgtg ggctgctttt ttagatagga
    23401 gaaaataaaa ataactattt aaacccgaac aaaaaggaat aatgcattaa aaatcattag
    23461 attttacaaa aaaataagta aaccacgttg tcagtttcag aaagtagtgt tagactgttt
    23521 tcaatatttg aaacaacatt ttttattcaa atatggcaaa ctgccttcac gtgcaaaaca
    23581 gttgcaagaa aatgaattta cactattatt agttttacat acctacttat atatcgtcat
    23641 aatatggatg acagtttcta gccagtaccg taaatgctgg actataagta aaagattggc
    23701 tatttcaccc ttcttttctt atgataaagt ggagggtgtt tttctttaac agaaacatag
    23761 taggtttttg cacaaaagcg gttatttcgg tattaataaa tgataaagga gcaaataaag
    23821 tgaaagaatt agttgaacgt attcaaaaag acggacgtat attaggcgag ggcgtattaa
    23881 aagtcgacag cttcgtcact caccaagtag accctgtatt gatggaacaa atgggaaaac
    23941 ggtttgcaga agtatttaaa gaacaaggaa tcacgaaagt tgtgaccatt gaagcttctg
    24001 ggatcgcacc agctttattt gctgcaagag aattagatgt tccaatgatc ttcgctagaa
    24061 aagcaaaaag tttgacgatg gatgaagaat tattgacagc ttctgtttat tcttttacga
    24121 aacaagtgac aagtacgatt tctatttctc gtaaattttt atcttcagaa gacaaagtat
    24181 tgattatcga cgatttcttg gcaaacggac aagcggcaaa aggattgatc gaattatgcc
    24241 agcaagctgg agcgcaagta gaaggaatcg ggatcgtcat cgaaaagtct ttccaagatg
    24301 gacgtcagtt acttgaagaa atgggattga gagtcgtttc tcttgcgcgt attgcttcct
    24361 tgacgaatgg acaagttgag tttcttgagg aggacgcata atcgtggaaa aagaaacaca
    24421 aaatggaaaa gctgcggttt tagggctgca gcatctgcta gcgatgtatg caggagcagt
    24481 agcggtgcct cttttgatcg gtacaggact aggctttaat caagaacaga tgacttattt
    24541 gatttcaatt gatatcttta tgtgtggatt agcgacatta ttgcaattga cagttaaccg
    24601 atttttcgga attggcttgc ctgtcgtttt aggatgtgcg atccaagcag tagctccact
    24661 gatcttgatc ggaaccaatg aaggagtagg agctatctac ggatctatta tcgcttctgg
    24721 gatctttgtt gtgttagttt ctggtgtctt ttctaaaatt aaaaaactat tccctcctat
    24781 agtaacagga acggttatca ccgttattgg tttgactttg attcctgtag caatcgagaa
    24841 aatgggtgga ggtaatgcaa cagcagctga ttttggtgac aaaacaaatc tgctacttgc
    24901 atttgtgacg attctcctaa ttgtaggcgt gcaaatgctg gcaaaagggt tcattcgttc
    24961 aatcgcggta ttgattggtt tagtcggagg aagcatctta gctgctttca tgggaatgat
    25021 tgatctgagc gcgattggag atgcgccgtt gttccatgtt ccacaaccct tttattttgg
    25081 taaaccaact ttcgacgtat ggtcgatttt actaatgatc atcatttcca tggtcagcat
    25141 ggtagaatca acaggtgtat attttgcact tggtgacatt actgggaaaa atgttggaga
    25201 agaagaattg aaaaagggat atcgtgcaga aggacttgcc gttatcttag gcggtatttt
    25261 caatacattc ccttatactg gcttttctca aaatgtcgga ttggttcaac tttcgggaat
    25321 caaaacaaga aaacccatct atttctctgc attcttcttg attgttttag gattattccc
    25381 taaaatcggt gcgttagcac aaatcatccc tgagccggta cttggtggag gaatgttagt
    25441 catgttcggg atggtggcag tccaaggaat ccgtatgctg ttggaagttg atttcagcaa
    25501 tgataaaaat ctattgattg ttgctgtttc tgttggattt gggctgggtt tcaatgttac
    25561 gccgacatta ttcagtcaaa tgccgcaaac attgcaaatg ttcactggaa atggcatcgt
    25621 aatgagtagc attacggcta tcatattgaa tttgatcttt aatggattga aaaaagatga
    25681 agaaatattg gaagaagcct tcaagaagta atagagcaaa agaaaggttg tgaaatcagc
    25741 gttttgacct gattgttaga gaaacaaacg gaaaaatagt tcctcgtatt ttgccgtttg
    25801 tttgagctaa taccgagggt cagctgattg aacaccgttg aaggaagagg tcgtgagcct
    25861 gccgttcatc tccgagtcac aaggaacaat ttcaaaaaaa agcttctcat gtttgttgaa
    25921 tattgttcct tgtgaccgag gagttggcag gagaacaccg tttatccata aaaaggaatg
    25981 tgacctaact tttgtcacat tccttttttt tatctttaat aaacacgaac aataaaatgt
    26041 ttttctttgt taacattcga aatttctttg acatcaaaaa aagtgattgt taaaatatga
    26101 atgtagaaaa actgggagga aaaacaatga cagcaaaaat ctctgtgatt atggggagta
    26161 cttcagattg ggaaacaatg aaagaagcat gtcaaatatt ggatgaattc ggtgtgtctt
    26221 atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt gatgtttgaa tatgcacaaa
    26281 acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc agggggagcc gcgcatctgc
    26341 cgggtatggt cgcatcaaaa acaactttgc cggttatcgg tgtaccggtc caatcacgaa
    26401 cattaaatgg tttggattcg cttctttcga ttgtccaaat gcctggagga gtacctgtag
    26461 ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg actacttgcg atacagatgc
    26521 tttccatgta tgatttagaa ttggaggcta aattggctga aagacgttca atgttggcta
    26581 aaactgttat agaaagtagt gatcaacttg gttaaacctt tgttgcctgg ttcaacgatc
    26641 gggatcgtcg gtggcggtca attaggtcgt atgatggcga ttagtgcaaa agaaatgggc
    26701 tttaaagtag gtgtgctgga tcctgtgagc gattgtccag ccgcacaagt tgcagattgg
    26761 cacattgaaa gtgtctatga tgacaccttt gcgctgaaag agcttgccag aagaacagat
    26821 gtcattacct atgaattcga aaacgtcagt atagaggcgt taaatgcgat tttacccatg
    26881 tcatttattc ctcaagggac agacttgctt gcgattacgc aagaccgcct gttagaaaaa
    26941 tcttttttgg aaacgaataa catcgtgatt gctccttatg caacgatcgt cagtccgacg
    27001 gatatccaag atgcgattga cgggatcggc tatccttgtg ttttgaaaac cactcgaggc
    27061 ggctatgatg gcaaggggca gtatgttttg aaaagtcggg cggatctagc tcctgccatg
    27121 gaccttttaa gagaaggaac ctgtgtttta gaagcatgga tcccgtttga aaaagagatt
    27181 tcgatcatgg tggcaggaaa tggtcaagga gatttcacga cttttccggt cgtggaaaac
    27241 atccaccaca acaatattct tcatgaaacg attgctccag ccgcgattga tcaggatgtg
    27301 atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag cagtgagcct ttcaggtgtt
    27361 ttaggggtag aaatgttttt aacaaaaaca ggcgggctat atgtgaatga attagctcct
    27421 cgtcctcaca attctggaca ttattctatt gaagcttgtt ctatgagtca gttcgatgcc
    27481 catattcgag gtatttgtgg ttggccatta ggagaaaaag tacgtttgat aagcaatgca
    27541 gtaatggtca atgttttggg agaacaactg ctggaaagct atcgcttgat tgagaataag
    27601 ccagactggc aattccattt ctatggaaaa aaacaagcaa aaaaaggtcg caagatggga
    27661 catatcacta tcttgacaga agacatccag caaacgctgg aagaaatcaa acaaacgaat
    27721 atttgggatt agaggagaaa aaacatgtta gaacgttata cacgccctga aatggggaat
    27781 atctggacgg aagagaacaa ataccgtgcg tggctcgaag tcgagatttt agcagatgaa
    27841 gcttgggcag aacttggtga aatcccgcaa gaagatgtga aaaaaattcg tgagcatgca
    27901 tcatttgata tcgatcggat cttggaaatc gaaaaattaa cacgccatga tgtcgttgcg
    27961 tttacacgag cggtttctga aacattggga gaagaaagaa aatgggtcca ttatggtcta
    28021 acaagtacgg atgtcgtaga tactgcttat ggctatctca tcaagcaagc aaatgatatt
    28081 ttacgggaag acttgaaacg ttttacggaa atcattggcg aaaaagcaaa agaacacaaa
    28141 tatacagtga tgatgggtcg gacacacggt gtccatgctg aaccaactac ttttggattg
    28201 aaacttgcca catggtattc agaaatgaaa cggaacatcg aacgatttga acatgcggca
    28261 aaaggagtag aagcaggaaa aatctcagga gcagtgggaa cgttcgcaaa tatcccgcca
    28321 tttattgaag agtatgtatg taaacaattg gggatccgtc cacaagaaat ttctactcaa
    28381 gttctccctc gggatctaca tgctgaatat ttctcagcga tggcgcttat tgctacaagt
    28441 attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat ccgaaacacg tgaagtagaa
    28501 gaattttttg cgaaaggtca aaaaggttct tctgctatgc cgcacaaaag gaatccgatc
    28561 ggttcagaaa atatggctgg tctagccaga gtggtgcgcg gacacatggt cactgcctat
    28621 gaaaatgtca cattatggca tgaaagagat atttctcatt cttcagcaga acgaatcatc
    28681 ttgcctgata ccacgatttt attgaattac atgttaaatc gttttggcaa tatcgttaaa
    28741 aatctaacgg tctttcctga aaacatgaag cggaatatgt ctgcaacatt tggtttgatt
    28801 tatagccagc gcgttatgct gaaattgatc gataagggaa tgacaagaga agaagcttat
    28861 gatcttgttc agccaaaaac tgcgtacgct tgggacaacc aagtgcagtt ccgcccgctg
    28921 ttagaagaag atccaaccat cacatcggta ttatctaaag aagaaataga cgatgccttt
    28981 gattatcatc atcatttgaa aaacgttgac gtgatattca aacgagtagg tttggaataa
    29041 agaagagtga gttgcaaaat tcaattaaaa tagaatagca aaaataacca aaaaagaagc
    29101 agatccgtta gcggttctgc ttcttttagt ttattatatt ttcttatcaa taataggaac
    29161 ctgattttaa aagcggctac ctctttaccg tgcctcttta catacaaaaa atagaggaat
    29221 taagcgtaaa tggcttggct attataacta aaatgctggt tatggagatg ttgcagcaag
    29281 ttttcgagaa aagcgtattc catctcttgt ccattgaata agatctggat acgataatga
    29341 tttttgaaac gcttctgttc atgaatctga ctgattgaaa gttcgccttt gtgttctgtc
    29401 tgataaagaa aagtcctttt acggcattca gcacagcgct tggttgctcg gcggattttt
    29461 cggaatgtat aaaaactagt atctttattg cataaataaa cgtctttgat cgacagcaaa
    29521 tcattatata gacaggaacg ttcttcagct gttaattctg ataaaaaaag ctgaccttgt
    29581 tttattgcat cttccatcgg caagtgcctc ctcgaaccca ttatccaaat ttttagttaa
    29641 ttctaatata ccatcattaa gtataaatgt aaacgcttgt tgatggtata agattttcgt
    29701 ttctgataaa acacgaacat taaaagaatc taatgcctta aatgtacata tttttgttga
    29761 tttataaaaa gatgattgct aaaatttaaa gtgaaaaaac gaacaaaaaa cgatgtattt
    29821 gcttttaatg taaataaaaa ggagagacgt acatggagaa gaaagaactt ttgtatgaag
    29881 gaaaagccaa aaaaatatat cttacttctg atgagaactt gatttggata gaatatttag
    29941 atcaagcaac tgcgttgaat ggcgcgagga aagaccagat aagtggaaaa ggagaattga
    30001 ataatcaaat cacaagtgca attttccgtt atctgactca aagaggaata aaaaatcatt
    30061 ttattgaaca attgtctaaa accgaacaat tagcaaaaaa agtacaaatt atccctctgg
    30121 aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa acgtctaggt gttcccgaag
    30181 gtaaagaatt catcaaacca atcattgaat tctactataa ggatgaccag ttggatgatc
    30241 catttatcaa tgaagagcat atctatttct tacaacttgc aaagccggaa gaaatcagtg
    30301 agctgaaagc ccaagcgttg gctattaatg aaattttgac cgaattattt gctaaggtcg
    30361 gtttggtttt agtagatttc aagctagaat ttggccggtc gaatggacaa attttgcttg
    30421 cggatgaagt aacaccggat acttgccgat tatgggatga acgtacgaat gaacatatgg
    30481 acaaagacgt ttaccgcaga gatctaggtg atttgatccc agtctatcaa gaagtatata
    30541 accgtctatc ggaactaact gaagaaaagg agcgagtatg atgtatttcg taaaagtata
    30601 tgtaacctat aaagattcag tgttagatcc tcaaggggag gcagtaaaaa aagcagtcca
    30661 tcgtatggga tatgaaacaa tcgaagatat ccgaatagga aaatactttg agatacaact
    30721 tgctgatgca gaagaaccaa tcgatacagt gattgaaacg atttgtgaca agctgcttgc
    30781 aaatgtaaat atggaaacat accgatatga aatcaaaaaa atggaggaag tataacatga
    30841 aatttgcggt catcgttttt ccaggttcaa attgtgacgc agatatgtta tgggcagttc
    30901 gcgagatcat gggcgctgat gccgaatttg tacggcatga tgagtcctct ttagaaggat
    30961 ttgatggggt tttactgccg ggaggattct cctatggtga ctatttgaga tgcggtgcga
    31021 ttgctcggtt ttctacaatc atggaagaag tgattcgttt tgctgaagaa gggaaaccag
    31081 tattcggtac atgcaatgga ttccagatat tgacagaagc aggacttttg ccaggtgtac
    31141 tgagacgaaa tgagtctttg cattttattt gcaaaactgt accattgaaa gtcaacccca
    31201 agacggcctt tacttcagag tatgaagccg acgaaataat ctatcttcca gttgctcatg
    31261 gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga actaaaaaaa aataatcaga
    31321 ttgcctttac ttataatgac aacatcaacg gcagcgtgga aaacattgca gggattacga
    31381 acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga acgtgcagta gaagacttgc
    31441 taggatcaga agatgggaaa cggttctttg cttccatttt aaaaaacttc ggaaaggtga
    31501 ccgtatagat gaaaacattg aaagaaccga cagctgaaga gatcaagaaa aaaagaatct
    31561 atgccgaatg gggattgact gatgaagaat atcgaatgat cgaagaagat atcttaggaa
    31621 gaatgcctaa ttatacggaa actggactgt tttctgttat gtggagcgaa cattgttcct
    31681 ataaaaattc taaaccggtt ctacgcaaat ttcctactag tggacctcat gtgttgcaag
    31741 gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg actagcagtt gtcttcaaag
    31801 cagaaagtca caatcatcct tcagccgtgg agccttatga aggagctgcg acaggagttg
    31861 gcggaatcat cagagatatt ttcagcatgg gggctcgtcc aattgcgttg ctagattcat
    31921 tgaggttcgg cgaattagat aatccaagaa cgaaatattt actagaagaa gtagttgcag
    31981 gtatcggcgg atacggcaat tgtatcggca taccgactgt aggcggcgaa attgcatttg
    32041 atccttgtta tgaaggaaac ccattagtca atgcaatgtg cgtaggtttg attgaacaca
    32101 aacatatcca gaaagggcaa gccgctggcg tcggtaattc gatcatgtat gtaggtgcaa
    32161 aaacaggtcg cgacggtatc cacggagcaa cttttgcctc agaagaattt aatcaagaag
    32221 aagaacagca acgttctgct gtacaagtag gcgatccttt tatggaaaag ctgctgcttg
    32281 aagcgtgttt ggaattaatc ttgcatcatt cggattctct aataggtata caggatatgg
    32341 gagcagcagg acttgtttca tctagtgcgg aaatggcttc aaaagctggc tcaggactta
    32401 tattgacatt agatgaagtc ccacaaagag aaactgggat gacaccttat gaaatgatgt
    32461 tatcggaatc acaagaacgg atgttgattt gtgttaaatc aggagaggaa gaaaaaatcc
    32521 aagaactatt tcagaagtat gatctagatg cagtcactat cggtaaagta acagacgacg
    32581 ggcaatatcg gctatatcat caaggagaag aagtagcaaa tctgccagtc gatgcattag
    32641 ctgaagatgc accagtttat cataaagaaa tgaaagaacc agctcgaatc gtagaatttc
    32701 agcagttagc tccttatcag ccagttatcg aagaacctgg acaagtattg cttgatctgc
    32761 tgcagcaacc gacgattgct tcaaaacgtt caatctacga aacttatgat tcacaagtac
    32821 aaaccaatac agttgtccgt ccaggaagcg atgctgctgt gatgagggtt agagggacga
    32881 ataaagcgtt agctatgacc acagactgca acgcgcgcta tctgtatttg gatccaaaaa
    32941 tcggaggaca aatagcagtg gcagaagctg ctagaaatat tattgctagc ggtggtaagc
    33001 ccttggcgat caccgactgc ctgaactatg gttctcctga taaaccagaa gttttttggg
    33061 aattatcgac ttcagctgac ggaattgctg ctgcgtgtga aacgttagga actcctgtta
    33121 tttcaggaaa tgtctcttta tataatgaaa cggatggtca agcaatctat ccaacaccaa
    33181 tgattggtat ggtcggattg attgaggatc ataaacatat cactactcag gaattcaaaa
    33241 aatcagggga tctaatctat atattaggta agacgtttgc agattttgat ggcagtgaat
    33301 tacaaaagat gcagcttgga agaatcgaag gtgtgatccg aaactttgat ctatccattg
    33361 aaaagagaaa ccaagagtta gtattgacag ccatccaaaa cggattgatt gaaagtgctc
    33421 atgactgttc agaaggtggc ttggcaattg cactggcaga atcagctttc aaacatcagc
    33481 tagggatatc tgtacagttt gaattgtcct ctgctcaatt atttgcagaa actcaatcaa
    33541 gatttgtgtt gacagtcgca ccggaaaata agacacgatt cgaagaaatg atgggagatg
    33601 ctgctgtttt agcaggtaag gtgacggatg aagcgataat cgaaatttca gcaactgacg
    33661 gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg ggaggatgca atcgtatgtc
    33721 ttatgaagtg aagagtttaa atgaagaatg cgggatcttc ggtgtttggg gtcatccaga
    33781 cgccgctagt gtggcatact tcgggctcca tagtcttcag catcgaggac aagaaggtgc
    33841 aggtatcgtg tccaatgatc gaggaaagct taaggggcat cgagatttgg ggctgctttc
    33901 tgaggtattc aaagatcaaa gaaaattgca gcagttaaca ggagaagctg caatcggaca
    33961 tgtacgttat gcaacagcag gcaatggaag tgtggacaat attcagcctt ttctattcaa
    34021 atttcatgat cagtcgatcg gcttagcgca taatggtaac ttgatcaatg ctcgttccct
    34081 tagaaaagag ttagaggaaa atggggcgat cttccattct aactccgata cggagatatt
    34141 gatgcatttg atccgacgaa gtgaagaaac agatttttta aataaattaa aaagtgcttt
    34201 gaatgaagtc aaaggtggct ttgcctattt attaatgaca gaaacggcaa tgattgccgc
    34261 tttggaccca aatggttttc gtccattagc aatcggacaa atgaaaaacg gagcatacgt
    34321 gattgcctcg gaaacttgtg cattagaagt tattggggca cgttttataa gagatgtcca
    34381 gcctggtgag atagtgatca tcaatgacga aggaatccag attgattgct ttacgaagga
    34441 cacgcagctt tctatttgtt cgatggaata tatctatttt gccagaccag attcaaacat
    34501 cgcagggatc aatgtgcata ctgcacggaa aaatatggga cggaatttag cgaaagaatc
    34561 gccagtaaaa gcagatatgg tcgttggcgt acctaattct tctttgtcag ccgcaagcgg
    34621 ttatgcggaa gctagtggaa tcccttatga aatcggatta gtaaaaaatc aatacattgc
    34681 ccgaacattt atccagccaa cgcctgagct aagagagcaa ggtgttcgaa tgaaattatc
    34741 tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg gtcgatgatt caattgttcg
    34801 gggtacgaca agcagacgga tcgtccatct tttgaaagaa gcaggggcaa aagaagtcca
    34861 tgtgaggatt gcttctcctc ctttgaaata tccatgtttt tacgggattg atattcaaac
    34921 gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa atcaaagaat gtatcggtgc
    34981 agattcttta ggttttttaa gtgaagaggg tctgatcgaa tcgatcggtc tggaaaaaga
    35041 tgctccgtat tcaggattgt gtatggctta tttcaacgga gattatccga cgccgttata
    35101 tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa gtatcgttct tttaaaagca
    35161 gaccaagaag ggagtatatg aattcaatgg caaatgcata cgcaaaagct ggcgtagatg
    35221 tcgaagctgg atatgaagta gtagaacgga tcaagaagca tgtcaaaaaa acggaacgtt
    35281 taggtatgat gggcgctata ggaggattcg gcggttgttt tgatttatcg gtctttcaat
    35341 taaaagagcc agtccttgtc tcggggaccg atggcgtggg gacgaaatta gcagtggcaa
    35401 tcgaagcaga caaacacgat acaatcggga tcgactgcgt agcgatgtgt gtcaatgata
    35461 tcttagcaca aggcgcagag ccgttatatt tcttagacta cttagcaacg gggaaaaatg
    35521 atccgttacg tttggaaaat gtagtaaaag gtgtagcaga tggatgtgtt caaggaaata
    35581 tggcgctgat tggcggagaa acagctgaaa tgcctggtat gtactcaggt aatgattatg
    35641 atctagccgg atttgctgta gggatagctg aaaaatctca attattgaca ggagaaacga
    35701 tcaacccagg agatgtgttg cttggaatag catctagcgg gattcattca aatgggtact
    35761 ctcttgttcg caaaattttc tttgagcagc agcagttttc tttatcatca aaactgccag
    35821 aattgaaaaa aacacttggc gaagaattgc ttgagccaac gaaaatctat gtttcttcct
    35881 tgcttccgct gatcaaagaa aagaaaataa aaggagctgc acatatcact ggtggagggt
    35941 ttgtagaaaa tatcccgcga atgttaccag aatctatggc tgcacaaatc aaacttggct
    36001 catggccgat tcttccaata ttcggctgtt tacaaaaata tggtgaaatt cctgagatgg
    36061 agatgtatga aatcttcaat atgggcatcg gtatggtcct ggcagtcgaa gaatcgaaag
    36121 taaaagaaat agtacacgaa attgagcaag ctggcgaaaa agtttatgaa atcgggaaag
    36181 tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga agaacgatga gaatcgctgt
    36241 ttttgcttct ggaaacggaa gcaactttca agcgctagcc gattatctat cgaaaaaagg
    36301 attggaatct tcgattgact ggttgttttg tgatcagcct gaagcatatg ttttgaaacg
    36361 tgcaactgcg ttgagtgtac cggcagattg tttttcacct aaagaatttg attcaaaaaa
    36421 agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa aagatcgatt tgatcgtatt
    36481 agctggatat atgcgaatca ttggaccggt attgctgaaa aattatgata aaagaatcat
    36541 caatatccat ccgtctcttt tacctgcctt ccctggtttg catggcatac gtgatgcctt
    36601 tgaagctgga gtaaaagaaa cgggtgtcac aattcattat atcgaccaag gtgtagatac
    36661 tggaccaatc atccggcagg aaaaagtgag aattgaacag gaggacacgt tcgattcttt
    36721 agaagaaaaa atccatagag tagagcatcg aatctatcca gaagtgatta gtgaaattat
    36781 tgaaaatgga ggctatctga aatgacaaga gcattgatca gtgtatcaga taaaactggg
    36841 attgtagcat tcgcccaagg tttgattgaa caaggagtag aaatcatttc aacaggagga
    36901 acaaagcatg cgttggataa agctgggatc aaaacgcttt cgatcgaaga agtgacaggg
    36961 tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc ctaaaatcca tggtggtctt
    37021 ttaggaagac gcgatctccc tagccatatg gaggcaatgg aaacggaaaa tatccaacca
    37081 atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa cgattatgaa accagatgtc
    37141 gaaatagcag aagctattga aaacattgat atcggtggtc ctagtatgct ccgtagtgca
    37201 gcaaaaaacc atgaatttgt cacctctgtt gttgatccat ctgactatga agaagtactt
    37261 tcagaattac gtcagtatgg gcatacttca ctttctttca gacaaaaact agcggcaaaa
    37321 gtctttcgtc atactgcggc atatgatgca ctgatcgctc aatacttaac ggattgggtg
    37381 gaagaaaaag aaccggaaaa attgacactt acgtatgaac gaaaacaaac gcttcgttac
    37441 ggtgaaaata gccatcaagc cgctgccttt tatcagtccg ctcttctgga aaaatattcg
    37501 atcgcagcag caaaacaact ccatgggaaa gaactttctt ataataatat ccgagatgca
    37561 gatgctgcgc tacggatcat gaaggaatat gacgagccaa cggtagtcgc cttgaaacac
    37621 atgaatcctt gtggtattgg gacagcatct acgattttag aagcgtggga agcagcgtat
    37681 gaagcagatc ctgtttcaat ctttggtggg atcatcgttt tgaatcgaga agtagatctg
    37741 ccaacagcag aacagatgca taagctgttt ctagaaatca tcattgcccc ttcttataca
    37801 caagaagctt tggaattact gcaaaaaaag aaaaacatcc gattactgac agtagacttt
    37861 gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag tcttaggtgg acttcttgta
    37921 caagatcaag atctggcaat agaaaatgaa gaagagtgga aagtcgtgac aaaacgtaag
    37981 ccgactccag cagaagcaaa agcattagtg tttgcttgga gagctgtcaa acatgtgaaa
    38041 tctaatgcga tcgtactggc aaacgaacgt cagacagtag gaatcggagc cgggcagatg
    38101 aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc aagagaaaat ggaaggtgca
    38161 gttttagcta gcgatgctta ttttccaatg gatgattcgg ttgaatatgc agccaaacat
    38221 ggtataaaag caatcattca gccaggagga agcatcaaag accaagattc tattgatatg
    38281 gcgaataaat acgatctagc aatggtcttt actgatgttc ggcattttag acattagaga
    38341 ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg gtcgtgaaca tgcaataagt
    38401 aaaaaacttt tagccagtcc gaaggtggat acggtgtatt gtgcgccagg aaatccgggg
    38461 atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc gagatcattc agcgttaatc
    38521 cattttgtta agaaagaggc tattgattgg agtatcgttg gtcctgaagt tcctttgtta
    38581 aatgggatcg tcgatgattt tatggcggaa ggattgaaaa tcttcggtcc gacaaaagca
    38641 gcagctctga tcgaaggttc gaaaagtttt gctaaacaat taatggatac ttatcaaatc
    38701 ccgacagcta aatccagaag ttttgaatgt tacgaaaaag cagcagcgta tatcaaagaa
    38761 aatggtgcgc caatcgtagt gaaagcggac ggcttagcag ctgggaaagg tgtagtcgtg
    38821 gctaccacag tgaaggaagc gctggatgcg gcagaacaaa tgttgaacca gcatcgattt
    38881 ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg caggagaaga attctccctt
    38941 ttggcgtttg tccgtaatac caaggtgtat ccgatggtca tttcgcaaga tcataagcgg
    39001 gcatttgata acgataaagg gccaaataca ggcggaatgg gtgcttattc accagtaccg
    39061 caaatttcaa aaaagatgat tgaaaaagct gtggaagaag ttttaaaacc agcggctttg
    39121 ggaatgaaag aactaggacg ctctttcacg ggtgttttat atgcgggttt gattgctacg
    39181 aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg gtgatcctga aacgcaagtc
    39241 gttctttctc ggttgaagag tgactttgcg caagtaatcg atgatttatt agagaatcgt
    39301 acagtagagt taaagtggca gcaagaagga tacaatgtag gcgttgtggt tgcagctgcc
    39361 ggttaccccg aagaatatga gacaggaatg gttctaccag attttctgga agaagagctt
    39421 tctgtttact atgctggagt ttcctcaaat aacggcaaac tagttggagc aggaggacgt
    39481 ctttatttag tagaagcata cggtgagacg atcaaagaag cacagaaaaa aatttacacc
    39541 gcgttaatgg ataaaacgac agatggaaca ttttatcgta cagatattgg agaaaaagct
    39601 gccaatagcg aactgatcgt gaagtaaatc agctgcttcc atttataaaa aatacaacaa
    39661 acataatcgg aagcgccgat tttagtgaag ccttttcata aagagtgtgg tacacttttg
    39721 ttgtgtatat aaataaggag gagttcagat gaaaaaaatt caattaggta cttctgatca
    39781 gaaagcagct gcagtgatct taggctgtat gcgtatcaac aatgcggaag atccagtaaa
    39841 agtgattgag acagcttatg aaaacggcat tgattttttt gaccatgcag atatttatgg
    39901 aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca aaaacatcaa tcaaacgaga
    39961 agatcttttt attcaaacaa aatgtggaat cgttccaggt gtgatgtttg atttttctaa
    40021 aaaacatatc attgaatctg tagaaaaaag tttgacccgt ctgaaaatgg attatgtgga
    40081 tgctttactt cttcatcgtc cagatacatt ggtagaacca gaggaagtag ctgaagcgtt
    40141 tgatgaattg gaacggtcag gaaaagttcg ttattttggc gtcagcaatc aaaaaccaat
    40201 gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta gtagctaatc agctgcaatt
    40261 tggcttgaaa catactggaa tgatcgatca agggattcat gtcaatatga cagatgaagc
    40321 aagtatcgat catgacggta gtgtacttga ttattcccga ttgcatgata tgacgatcca
    40381 agcatggtca ccataccagt atggattttt tgaaggtgta tttatcggga acgaaaaatt
    40441 ccctgaatta aaccaaacat tagaagaact agcagctaaa tatcaaacga caccaactgg
    40501 cttggcttct gcttggattt tacgccatcc agccaatatg caagtgatcg ccggtacgat
    40561 gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa attaatatgt ctcgggaaga
    40621 ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct taaagattgt cataagcagg
    40681 tcaaaagggc tataaattaa aaagacagaa taacttgaaa gctgttttca gcttcgagat
    40741 attctgtctt tttttattgc aaatggaaag agtatcattg tacggatttc gtttattagt
    40801 tatttttgtt tttaaggaaa agctgtgtcc gatattcttt tggagaaatc ccttgttttt
    40861 tcttaaataa ataactgaaa tagttagggt cattataacc aatttcaaaa gcaatatctg
    40921 aaatacgata gtctgattgc tgcaataatt tttttgctaa ttcgattctt tgttctgtca
    40981 gataatcgat gaatgtttgg ttaaacgatt gagaaaaaat cgtactgaaa tgagagggac
    41041 ttaatgcaac ttgttgagcg acccaagtta aggacatatc tggatcggta aaattttctt
    41101 tgataaaatc caatgcatgg ttcaaaattg gctgatattt gttccttgaa ggctgtgagg
    41161 gcagagcagt taagaaagaa atgaacatat aaactgtcga acggtaaagc gagtcgtcgg
    41221 cgctgattgc taagcgttga ctggtggatg tgcaatgaac catttcttgc gggattttct
    41281 tttctttcag gcagacatgc tggagtagct tagtcaagac aaataaacga tataatcgtg
    41341 ttcgagttgg tgtgccttgt ggtttcgtca agtgttcgat gtacttagat acttcgtttt
    41401 tcgataatga agacaaatca agtgagaggt caaagtaaaa gacagaatca actggaagtt
    41461 tcgcttcttg aatcaaatcc tcatagctga taatttgttc tttaagggaa ctggcggttt
    41521 caagtatcat ctgcgtgatc tcaaaagatg caggtaattc gctgattctt tctgcgacag
    41581 gaccaatggc gacagaaaaa ttattttttt gtcccttttc taattcatgg accaacgttt
    41641 gggctacctg ataactggtc tcgatcacat tttcttcaag agaatcaact attaaaaatt
    41701 tgatgaatcg agaggaaaca gcagagaaga tcacttgttg attctcccta aataaaaact
    41761 tcaaataatc actgagatga gcgtaatctt caaagtctgt actgacttgg tttgctgcta
    41821 ataatacggt catttttttc cctgctaaag agcgattgaa agattcagca agtttcaatg
    41881 tttcactgat cgtaagtgag cctttgaaca aaccgttcaa gaaatgattt tttttcaatt
    41941 cgaaaacaag atcagaagaa gtgtgcgttt ctttttgctg caaatctaaa gcagttactg
    42001 ctttttctat tgcttgtttc aattcgctac ttttaatcgg tttaagcaga taatcatctg
    42061 cctgtacctg gattgctcct ttcatataat cgaaatcgtc gaatccacta ataaatatga
    42121 gacgagtcca aggaagtagt ttccttgctt cttttgaaaa agtcaacccg tccatgaaag
    42181 gcatttggat atcagtcaga atgatgtcgg gctgtaagtc gataattgaa gacagtgcca
    42241 tctcaccatc tccagcttcg ccgacataag tgattggata ataagaacca aggacagtta
    42301 tttgttcccg caagttattg cgaatgaggt attcgtcttc gatgataaaa actttataca
    42361 tgtttgtttc ctccaattgt tttgggagat agagggatca tcagtgtgac agttgttcct
    42421 tctcggtatt tgctgtcaat cgtgataggt ttttgatcat ttccgtaata aagaaggaga
    42481 cgcttgttta cgttgtaaag tccgtagccg atactgaaat ctgaatcgat ccctttggct
    42541 aactctttac gtatttctgc cagtttttct gcagggatgc caatcccgtt gtctataact
    42601 ttgaattgga tcatctcatc aattttgcat gtggtaatac gaataaatcc tttgtgacgg
    42661 acgtacttcg ttccatggta tacagcattt tcaatcaatg gctgcaagat catttttaaa
    42721 atagaaacag gaaaaagtgt ttctggtata tcgatcttat agtcaagcat atggccgtat
    42781 cttattttta agatgactag ataatcattg atatggctaa tttctttttc aagaggaatc
    42841 cagtcttgtc ctttgcttaa agacgtacga aaaaagccgg aaagtgcatg ggtcatagtc
    42901 agagcttctt caaagcagtc ttgttcgatc aaagaaataa tggcatccaa actgttataa
    42961 ataaaatggg gagtaatctg agcttgcaat acacgaactt cactttgtgc taaatggtat
    43021 tgtttcaaag cgttctcccg taaagactgg tttagatctt ccgccatttt attcatgcta
    43081 gcggtaagtg tttgtaattc cagtgtttcc ggcagggtta aacgaaagcc aaggttccct
    43141 gaagaaagtt gttctgacat ctcgatcagc tgattgatcg gttcttggac ctttctctct
    43201 aaaaagcgac gattcttttt cgcatagaaa aaaatggtac taagaactcc aagttccact
    43261 attgttaata gaatgagcga gcggacaagt gtcgtatttt ttttgcttgc taagtcgatc
    43321 tccacacgaa caaaatcttg taagatatct gacagcagtt gagtgacaga atctacctga
    43381 ttgatgatct cttcattggt atccataaaa tcttttgcca agatattatt gatcagctcg
    43441 ttttgataat cttccagtgt atcgatcgta cgtagcgata catctaacgt actaatttct
    43501 ttaagtgtat ttgtattatt tttgatctct cgaatatcat tcctaagttc ttctaggata
    43561 ttttcctcat tgaactcatc tacggttact tggccagaaa tcaagtccca catactttcc
    43621 aaaacttttt gatcaacctt caaagcaatt gcatctgctt tttggatatt tgcgatcgtc
    43681 tgttgataaa tcaaaagctg ttgcgaatat aacagtatgc tcagtagcaa agggataaca
    43741 gcaaatagaa gcatggcttt gtttgcttgg gcaaaaatct gttttatgct taaatttttt
    43801 gtcatgatac ttctccagtc aataatttct ggttgggatg ttagctatat tctgctggga
    43861 aaagacagtc tcggaaataa aaatatcttt aggtaatgtt tttcctgttt gaatatttcc
    43921 aaaataacga gtaacggtat tggctacata ccagccgaca tcaggtttac actcgactac
    43981 acaattgacg attcctgagc gtagcttctc gatcatatct tcttgtccat caatagttac
    44041 aatgatcaaa tcttctccag gtttgattga tgtttctttt atcgcagcta aagcaccgtg
    44101 agtcatttca tcactatggc tgtataatac atcgatttcg gataaatcat ttgtttctaa
    44161 atatcgtttc atctggtctt ttcctttcat ccgaataaaa tcaccgacaa ttcggtgaac
    44221 aatagtcatc ggaggtttat ggtcgatggt tccatctttt tttagggttg gtttacgatt
    44281 gattgattcc aaaaatcctt cggagcgtag cttggtcggt gtggagttct ctgaaccagc
    44341 aagttctagg attctgattt cctgtttctt tgaatttgtg aagtgattgc tgacaaataa
    44401 acccgcccga tttccttctg ctttgaaact ggggccaatg tgggtgacga aaagctcggg
    44461 atcattcgtt tgaatatgtc tgtcgacaag tatgacaggg atccctgctc gctttgcttc
    44521 ttttaataca gagtcccacc cggattcttg caaaggagta aaaatgatca tatccacttt
    44581 ataggcaatg aacgtccgta tatcttggat ttgccgttcc tgattcatgt agccatttcg
    44641 gtagagaact tggtaatctt ctttttcaag cgcagtgata attgattcag tatgcttttt
    44701 ccgccagcta ctctccgttc ctgattgcga aaaaccaacg actagtgggt caggtgcttc
    44761 ctttgtatta ggtgtacacc ccatcatttg gtaagcgatt acaacaaggg ctaaaaaaaa
    44821 taaaacacgt ttcactttaa atcactccat ctggatttgt tagtactatc ttatcagata
    44881 aaagggaaaa tcaaagtcaa aaggaatcaa tcgaaaaaaa tccaagaaac ttaaaaaaat
    44941 ccaagacaaa aaaagtacac ctaaaaaatt taaagtaaat cccataaaaa aatcaatgtt
    45001 aacgctttct ttattagcta tactcggtgt atcaattgaa atgggggata aaaaatgaaa
    45061 aacaaattta gaaagattgt tgggactatc gctattttga ccgcaagcat tctattaagt
    45121 gcgtgtggca attcaggatc tgcagatgat agtacggggt atgtaggaat cgctatgccg
    45181 accaaatcag cagaaagatg gatcgctgat gggaacaaca tggtctcgga attagaaaaa
    45241 ctgggttata aaacagatct tcaatatgga gaagataaag tagaaaatca agtagcacaa
    45301 atagaaaata tgattacaaa aggggtagat acacttgtga ttgcttctat cgatggttcg
    45361 gctttaactg atgtgttagc taaagcaaaa gaagcagata tcaaggtgat cgcctatgat
    45421 cgtttactga tgaattcaga aaatgtcgat tattacgcga cttttgataa ttttggagta
    45481 ggtgtatcgc aagcagctta tattgaagag cacttaggat tgaaagaagg aaaaggacca
    45541 ttcacgattg aattatttgg tggctcgcct gacgacaaca acgcactaat caattacaac
    45601 ggagtgatgt ctgttttaca accatatatg gacaacggtc agttggtcgt gccatctgga
    45661 cagacgagtt tcagtcaaat cgctacgctt cgctgggatg gttctacggc gcaagcaagg
    45721 atggataatc tgttaagcgc aaattatact gaccaaacat tggatgctgt tttgtcgcca
    45781 tatgatccaa taagtttagg aatcatttcg tcattaaaag gcgtggggta tgggagtgaa
    45841 agtaaacctt tacctgtgat tactggacaa gatgcgacag ttgctggcgt taaatcgatc
    45901 attgccgggg aacaaacaca aacgatcttt aaagacacac gtatcttagc aaaaaatacg
    45961 attgaaatga tcaaagcaat cagtgatgga gaagaggttc cggtcaatga tactgaaact
    46021 tatgataatg gggtgaaaac tgtccctacg tatttagcaa atacagtatc tgtcgataaa
    46081 gataattatc aagcagagct gatcgacacc gattattata aagaatccga tctgaaaaat
    46141 taagaggaac caaacaagga actaaagaga tctaagtcag agagaggata agaattatgg
    46201 cggattgtat tttaaaaatg gcagatatca ccaaaaaatt ttctggaatc cgagcgttag
    46261 attgcgtgaa tctgatgatt gaacgtgggg aaatccatgc attgtgcgga gaaaacggag
    46321 cgggaaaatc aacattgatg aatgtcttga gcggcttgta tccatatggc tcctatgacg
    46381 gggaaatctt ttatgatgga aagctgtgtc aatttaaaga tttgaaagat agtgaagaaa
    46441 aaggcatcgt catcattcat caagaattag cactaagtcc ttatctttca ataaaagaga
    46501 atatttttct cggaaatgaa caagcagaaa gaggcgtgat tgattgggaa ctgacagaaa
    46561 agaaaacata tgaactgctg aataaagtcg gtttgtctat cgacccgaat cttctagtat
    46621 cccagattgg cgtagggcaa cagcaactag ttgaaatagc taaagcatta tcaaaatccg
    46681 tccgtctgtt gattttggat gagcctaccg ctgcattgaa tgaagaagaa agcgcgaatt
    46741 tgctgagatt gatccgagag ttgaaagagc aaggtgtaac ctccatcatt atttcacata
    46801 agctgaatga aatcgtggat atcgcagatc gaatcaccat tttacgggat ggacagacga
    46861 ttgagacact agaaaatgaa atgatcagcg aagagcatat tattcgcgga atggtgggca
    46921 gagatttagt tgatcgttat cctgaaagaa aaccggatat tggagaagtt tattttaaag
    46981 tagaagactg gacggtctat caccctcttg atcacgagcg aatcataaac gaccacatca
    47041 acttgcattt gaaaaaagga gagattgtag gaatagctgg actgatggga gcgggcagaa
    47101 ctgaattcgc tatgagtcta tttggacatt cttatggaag caagatatcc ggacgtatat
    47161 taaaggaagg ggcagagata tcagttaaga ccgttccttc agccattgca aacggactcg
    47221 cttatgtctc ggaggaccga aaatctttag gactgaatct gctgatggat attcgggaaa
    47281 acgtgacgct gagcagtttg gataaaataa gtcaaaaagg agtattagat aaagaaaaag
    47341 aagtaactga ggcagaaaat ttccggaaaa agatgcgtat aaaaacaaac tccatccatc
    47401 aaattgtcag tagtcttagt ggtgggaatc aacaaaaagt cgttttatcc aagtggctga
    47461 tgacacaacc cgatattctt tttttggatg agccaacccg tgggatagat gttggggcaa
    47521 aatatgagat ctacacgatt atcgaagaaa tggcagcaaa tggcaaagct gtctgtctta
    47581 tctcttctga acttcctgaa atattaggga tgtgtgatcg aatctatacg atgaacgaag
    47641 gacgtattac aggagaagtc tcgcgagaag atgcagatca ggaagtcttg atgaaattaa
    47701 tgacgaaaga agaggtgggt taagttggaa caggcaaaaa atcttacgga taaaaaagag
    47761 aaaaaactaa atttgaaaag tagagcagct gatctttttg gtcgctacag catggtgatc
    47821 attttagcgg gactacttat tgcgtttcaa ttgatgacgg atggtatttt ctggcgtcca
    47881 ttgaatataa ctaaccttgt tctgcaaaat agccatatat tagtactagc tgcagggatg
    47941 ttattagttg tgctcttagg tcatgtggat ttgtcggtag ggtcagtgat ggcgtttgtt
    48001 ggtgcaattg cgggcatgat gatggtcaat cgccaaatga acccgtggat gacagttatc
    48061 gcatgtcttg cagtaggcgg gctgatcggc gcatggcaag gtttttgggt agcttatgca
    48121 gggataccgg cgtttatcgt gactttagca ggattgctga tgtttcgtgg actgacacaa
    48181 gttgttctag gaggccaatc tctagcacct tttccagctg ttttccaaaa aatgtctacg
    48241 ggttatttac cagacttaag cgaaggaagc agccttcact tcttgacatt gattttagga
    48301 atgatcatca cgatcgtgtt agttgttggt caatggcgaa caagaaatcg tcggaaaaat
    48361 catttatttg aagtggaatc tccaacagct ttcttggtaa aggcggtatt tacagcagtc
    48421 gttgtactag gaataactta tatctttgcc tcttatcaag ggtatccgat tattttgatc
    48481 attcttggtt tgatcgttgc agcatatggg tttttgacaa ataaaacagt agcaggtcga
    48541 cagatttatg cgacgggcgg aaatcgaaaa gcggctgaac tatcagggat caaaacaaag
    48601 cgaatcacgt tctgggtatt cgtcaacatg ggagtcatgg cagctttagc tggattagtt
    48661 ttagctgctc gattgaatgc ggcaacacct caagcaggta catcgatgga actggatgca
    48721 atggcggcag tatattttgg tggtgcttcg acttccggcg gcattggaac aatcatgggt
    48781 gcgattgtcg gtggcttggt catgggtgta ttgaacaatg gcatgtcgat tttaggcgtc
    48841 ggggtagact ggcagcaggc aatcaaagga ttgatcttgc ttttagccgt tgtgctagac
    48901 ttgtataata agaaaaaacg aacaacgtag gagaacgccg gttattcaga aaaaagactg
    48961 tgacaagaca tttgtcacag tctttttttc gctaaaagcg aacattcatt gtttcgtttg
    49021 tatagactaa aaataatata tgttttttta ccattcgtat aaattctctt tttgctcttg
    49081 atttttttgc ttaattgagt taaattacat agggcgctaa aaaaagcctt gattaaatta
    49141 ataatgttcg tgttttgctg gatgtgaaca tcaggaggat aatgtaatga aagtatttga
    49201 ttacgaagat attcaattaa ttcctaataa atgtatcgta aatagccgtt ctgaatgtga
    49261 tacaacggtc acattaggta aacatacatt caaaatgcca gtagtgcctg ccaacatgca
    49321 gacgattatt gatgaaacaa tcgcagaatt cttagcggaa aatggttatt tctatatcat
    49381 gcatcggttt gatgaagcag ctcgaattcc gtttatcaaa aaaatgaaaa agcgtggatt
    49441 gattacctcg atcagtgttg gcgtcaagaa agaggaatat tcctttatag agaagttggc
    49501 agaagaatcg ctaaatcctg attatatcac tatcgacatc gcgcatggac atgctaactc
    49561 agttattgat atgattcagc atattaaaaa atatttgcct gaaacttttg tgattgccgg
    49621 taatgttggt acaccggaag ccgtccgtga attggaaaat gcgggcgcag atgcaacaaa
    49681 agtcgggatt ggtccaggaa aagtatgtat tacaaaaatc aagacaggct ttgggactgg
    49741 aggctggcaa ctggcagctc ttagatggtg tgcgaaagca gctcgcaagc cgatcatcgc
    49801 tgacggtggg atcagaacac atggcgatat tgcaaaatct gtccgttttg gtgcgacaat
    49861 ggtcatgatt ggttcattgt ttgccggtca cgaggaatct cctggcgaaa caaaagtcga
    49921 aaatggaatc gtgtataaag aatactttgg ttctgcttca gaattccaaa aaggcgaaaa
    49981 acgaaatgtt gaaggaaaga aaatctggat ccaacacaaa ggatctttga aagatacact
    50041 agtggaaatg caacaagacc ttcaatcatc tatttcttat gctggaggaa gagatctgga
    50101 agccattcga aaagttgact atgtcatcgt gaaaaattcg atttttaatg gagacacaat
    50161 ctaaaaaata aataaaatat taggatgttg tgaaaaagtt gtcctgcatt aagtaataag
    50221 aacagccaaa caaaaatagt tctttatatg ttttgtttgg ctggaattat tatcgcagat
    50281 acaagctttt gaacatcatt attcagaata agggactgtg ataagatttt tgtcacagtc
    50341 ccttatgttt tgcaaaaaaa gtttttcttt tttctcgtaa attcggtaaa atcaaaagaa
    50401 aaaatagaca atagaaaaga gaagcttatg tgggaaaaag tagagaaaaa acaaggaccg
    50461 atttatcgtc aaattatgga acagattatg agagaaatcg aaaacggaag gatcaatcct
    50521 ggaagtcaac ttgaatcaga acgaaaacta gggcaacttt ttggtgtgaa tcgttccacc
    50581 gttgtccatg cattggaaga gcttagggag cttggggtac taacgagcaa acgtggcagc
    50641 ggatggtttg ttaatcagac tgaatggggg aaattcgctg ttccaagaat cgattggcgg
    50701 caaatgatct ctcctcgtta tgagcagacg gactggtatg agcaaaaaat aagagaagca
    50761 aagcagatcc aaaaaacatc gtttcttgat ttatacgcta gcgagatgcc agatgagctt
    50821 ttgccgaatt ttgagtttcc ttcttattct cttgaagaaa tcttagcgga agaaaaagaa
    50881 atggatcttt taggctatac tccgttacgt aacaaaatca aacggtattt ggaaagtaaa
    50941 tttgaattgg cattttcccc caaccagctt cttgtgactg gtggcggtca gcaagcaatc
    51001 tttttgattt tacaaacgat tctttcagct ggagagggga tagctgtaga gtcaccttct
    51061 tttttttacc gattacctct tttcaaagct tcaggaacgc gtttatttgg gataccgatg
    51121 gatgaagaag ggatcgactt agaaaaactg aaagaatcga tccataaaaa taaattgaaa
    51181 gcagtgctta tcaacccgaa ttttcaaaac cctacaggaa aagtgatgag tcaaaaacga
    51241 cgagaaaagc tagtagaatt gtgtcgacag tatcggcttc caattattga agacgatgta
    51301 tttacggatc tttcatttct aaaccaagaa gaagtccttc ctatacgaac gattgatccg
    51361 gataatgtgt tatacgtcgg ttcgttatct agggttttag gaaaaacaac gaaaatcggt
    51421 tgggtgatag gacctgagac ttttatctcg caactagctc aagcgcaaga aatgatggag
    51481 ttttcgatga atatccttac acaaatcaca gtggctaatg tatttaatga agcaattgat
    51541 ttgaaaatgc aagatgtaag aaaaaagttg aaagaaaaaa gccgactgat aagcgcttgg
    51601 ggagattcac aatcgttttt caagcttcat tcaattttag gtggttacta tgcctggatt
    51661 acttgggatg ggaaaaaact agaccccgaa ctagcagaaa aagtagtaga tatgggtctt
    51721 ggaattgcac caagtctact ttttgggcaa gatataaatg gaatcagaat caattacagt
    51781 aggctggatg aaaaccaaat accgttgttt tattcaaaga tgaaaattct agaaaagtgg
    51841 ttaacagaat aatttcacaa agtaaataga ttaaagaata taacagcttg gatttaaatt
    51901 tcaagctgtt tctttttttt cggaaaaaga atacgatata aagaaactaa gaaaaaacca
    51961 ttatttgtac gtccgttcac gatgtgtaaa taaaataaat gtttgcaaca aaactgtata
    52021 acaccaacgt ttttaaatag aaacagatgg ttgtaagttt gattagaaaa tagaaaaaat
    52081 gcagatgaaa tataaaacgc tttccttatt gtgattttat tcactaatgc tgttaatatg
    52141 cggattggca taatttcctt tcatcgtgaa aatttgtgct tttagcttgt atttctcata
    52201 aatccatgat aatttattaa tgaagacggt taaatagtga tacagtttta ttcactgttt
    52261 caatcgcaga atgaagagat tttcttatag atgagagagg aatgtgtgac aaatggcaaa
    52321 caaaaaagca ccgatcgact tccaagcact gctagatgaa gtcaatgcag atttcccagt
    52381 atatcaagca cttgatcaag atggaaacgt agttaacaaa gatttagtac ctgatttatc
    52441 cgatgatgaa ctcgttgaat taatgactca tatggtatgg tctcgtgtat tagaccaacg
    52501 ttcaacagcg ctgaaccgcc aaggacgcct aggctttttt gcaccaactg ctggacaaga
    52561 ggcaagtcag ctggctagtg cttacgcatt tgataaagaa gatgttcttc ttccaggtta
    52621 tcgggatgta cctcaactag taaaacatgg tttaccttta tctcaagctt ttttatggtc
    52681 tcgaggacat gctgcaggta attcttatcc ggaagaattg aaagctctac caccacaaat
    52741 tatcatcggt gcacaatatg tacaagcagc cggtgttgca ttaggtttga agaaacgaaa
    52801 caagaaaaat gtcgtcttca cttatactgg tgatggcggt tcatcacaag gtgattttta
    52861 cgaagcaatc aatttcgctg gcgcttacca tgcaaatgcc gtattttata tccaaaacaa
    52921 cgggtatgcg atctctacac ctcgtgaaaa acaaactgca gcaaaaacac ttgcacaaaa
    52981 agctgtcgca gctgggattc ctggtattca agtcgatgga atggatccgc ttgcagtata
    53041 tactgtatct aaaatggcac gggaatgggc gatcagtgga aatggaccag tcctaatcga
    53101 aacactgact tatcgttatg gaccacatac actatctggt gacgatccaa cccgctaccg
    53161 tacaaaagac acagatgacg attggcagaa aaaagatcca ttgatccgct tccgtaaata
    53221 tttgacggaa aaaggacttt ggtctgaaga aaaagaagaa caagtcatcg aagcaactaa
    53281 agaagaaatc aaagcagcta ttgctgaagc cgacaaagta ccaaaacaaa aggtttcaga
    53341 tttcttgaaa aacatgttcg aagtatctcc acaatcaatc aaagaacaaa ttgcaattta
    53401 tgaagcgaag gagtcgaaat aatcatggca caaaaaacaa tgatccaagc aatcacagat
    53461 gccttagcac ttgaactaga aaatgacgag aatgtcgttg tttttggtga agatgttggg
    53521 aaaaacggag gagttttccg ggcaactgaa ggattgcagg aaaagttcgg agaagaccga
    53581 gtattcgata ctcctttagc tgaatctgga atcgcaggat tgagtttcgg tcttgcgtta
    53641 gaaggatttc gtccagttcc tgaaatccaa ttctttggtt ttattttcga agcaatggac
    53701 gaagtagtag ctcaaatggc tcgtacaaga tatcgaatga gcggcactag aaatctaccc
    53761 atcacaattc gttcgccatt tggtggcggt gttcatacac cagaattgca ttcggataat
    53821 ttagaaggtt tgatcgctca gtctcctggt attcgagtag tgattccttc caacccttat
    53881 gatgcaaaag gactattgat ttcttctatt agaagtaacg atccggttgt tttcctagaa
    53941 cacatgaaac tatatcgctc attccgagaa gaagtgccgg atgaagctta cgaagttcct
    54001 ttggataaag cagcagttac tcgtgaggga acagatgtat ctattatcac atacggagca
    54061 atggtgcgtg aagcaatcaa agcagcagac aatcttgcaa aagaaaatat atctgttgaa
    54121 atcattgatc ttcgtacagt tgcgccatta gatgtagaaa cgatcattca atccgtggaa
    54181 aaaactggtc gcgtagttgt tgtccaagaa gcacaacgac aagcgggcgt tgccgcgcaa
    54241 gtagtttctg agatctcaga acgcgctatc ctttcattag aagccccaat cggacgagtt
    54301 tctgcacctg atacagtctt cccatttgga caagcagaaa atgtctggtt gccaaatgca
    54361 aaagatatcg aagacaaagt aaaagaaatc gcggaatttt aagaaaagaa gggacgaaac
    54421 aaagtcatgg cttatcaatt taaatt
//
LOCUS       DACCCS010000014        51057 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.014, whole genome shotgun sequence.
ACCESSION   DACCCS010000014 DACCCS010000000
VERSION     DACCCS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.014"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(177..971)
                     /locus_tag="IVK82_000860"
     CDS             complement(177..971)
                     /locus_tag="IVK82_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAP6391938.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
     gene            1590..4424
                     /locus_tag="IVK82_000861"
     CDS             1590..4424
                     /locus_tag="IVK82_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6391939.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            4615..5106
                     /locus_tag="IVK82_000862"
     CDS             4615..5106
                     /locus_tag="IVK82_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP6391940.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            5209..6198
                     /gene="manX"
                     /locus_tag="IVK82_000863"
     CDS             5209..6198
                     /gene="manX"
                     /locus_tag="IVK82_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP6391941.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            6231..7034
                     /locus_tag="IVK82_000864"
     CDS             6231..7034
                     /locus_tag="IVK82_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP6391942.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            7055..7969
                     /locus_tag="IVK82_000865"
     CDS             7055..7969
                     /locus_tag="IVK82_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP6391943.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            8052..8426
                     /locus_tag="IVK82_000866"
     CDS             8052..8426
                     /locus_tag="IVK82_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP6391944.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            8565..8810
                     /locus_tag="IVK82_000867"
     CDS             8565..8810
                     /locus_tag="IVK82_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6391945.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            8982..10046
                     /locus_tag="IVK82_000868"
     CDS             8982..10046
                     /locus_tag="IVK82_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP6391946.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            10292..10717
                     /locus_tag="IVK82_000869"
     CDS             10292..10717
                     /locus_tag="IVK82_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6391947.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            10710..11159
                     /gene="nusB"
                     /locus_tag="IVK82_000870"
     CDS             10710..11159
                     /gene="nusB"
                     /locus_tag="IVK82_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP6391948.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            11256..12110
                     /locus_tag="IVK82_000871"
     CDS             11256..12110
                     /locus_tag="IVK82_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP6391949.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            12111..13448
                     /locus_tag="IVK82_000872"
     CDS             12111..13448
                     /locus_tag="IVK82_000872"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP6391950.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            13453..13677
                     /locus_tag="IVK82_000873"
     CDS             13453..13677
                     /locus_tag="IVK82_000873"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP6391951.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            13679..14569
                     /locus_tag="IVK82_000874"
     CDS             13679..14569
                     /locus_tag="IVK82_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP6391952.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            14578..15396
                     /locus_tag="IVK82_000875"
     CDS             14578..15396
                     /locus_tag="IVK82_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP6391953.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            15503..15952
                     /locus_tag="IVK82_000876"
     CDS             15503..15952
                     /locus_tag="IVK82_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP6391954.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            15970..17652
                     /gene="recN"
                     /locus_tag="IVK82_000877"
     CDS             15970..17652
                     /gene="recN"
                     /locus_tag="IVK82_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP6391955.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(17707..17823)
                     /locus_tag="IVK82_000878"
     CDS             complement(17707..17823)
                     /locus_tag="IVK82_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP6391956.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(17896..18852)
                     /locus_tag="IVK82_000879"
     CDS             complement(17896..18852)
                     /locus_tag="IVK82_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6391957.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            19023..19391
                     /locus_tag="IVK82_000880"
     CDS             19023..19391
                     /locus_tag="IVK82_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP6391958.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            19561..19992
                     /gene="mraZ"
                     /locus_tag="IVK82_000881"
     CDS             19561..19992
                     /gene="mraZ"
                     /locus_tag="IVK82_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP6391959.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            20013..20972
                     /gene="rsmH"
                     /locus_tag="IVK82_000882"
     CDS             20013..20972
                     /gene="rsmH"
                     /locus_tag="IVK82_000882"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP6391960.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            20977..21378
                     /gene="ftsL"
                     /locus_tag="IVK82_000883"
     CDS             20977..21378
                     /gene="ftsL"
                     /locus_tag="IVK82_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP6391961.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            21379..23571
                     /locus_tag="IVK82_000884"
     CDS             21379..23571
                     /locus_tag="IVK82_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP6391962.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            23597..24559
                     /locus_tag="IVK82_000885"
     CDS             23597..24559
                     /locus_tag="IVK82_000885"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP6391963.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            24573..25934
                     /locus_tag="IVK82_000886"
     CDS             24573..25934
                     /locus_tag="IVK82_000886"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP6391964.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            25960..27048
                     /gene="murG"
                     /locus_tag="IVK82_000887"
     CDS             25960..27048
                     /gene="murG"
                     /locus_tag="IVK82_000887"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP6391965.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            27063..28283
                     /locus_tag="IVK82_000888"
     CDS             27063..28283
                     /locus_tag="IVK82_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP6391966.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            28439..29764
                     /gene="ftsA"
                     /locus_tag="IVK82_000889"
     CDS             28439..29764
                     /gene="ftsA"
                     /locus_tag="IVK82_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP6391967.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            29787..31028
                     /gene="ftsZ"
                     /locus_tag="IVK82_000890"
     CDS             29787..31028
                     /gene="ftsZ"
                     /locus_tag="IVK82_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP6391968.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            31045..31722
                     /locus_tag="IVK82_000891"
     CDS             31045..31722
                     /locus_tag="IVK82_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP6391969.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            31736..32335
                     /locus_tag="IVK82_000892"
     CDS             31736..32335
                     /locus_tag="IVK82_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP6391970.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            32350..32637
                     /locus_tag="IVK82_000893"
     CDS             32350..32637
                     /locus_tag="IVK82_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP6391971.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            32686..33468
                     /locus_tag="IVK82_000894"
     CDS             32686..33468
                     /locus_tag="IVK82_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP6391972.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            33489..34196
                     /locus_tag="IVK82_000895"
     CDS             33489..34196
                     /locus_tag="IVK82_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP6391973.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            34458..37256
                     /gene="ileS"
                     /locus_tag="IVK82_000896"
     CDS             34458..37256
                     /gene="ileS"
                     /locus_tag="IVK82_000896"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP6391974.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(37305..38831)
                     /locus_tag="IVK82_000897"
     CDS             complement(37305..38831)
                     /locus_tag="IVK82_000897"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP6391975.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(38846..39493)
                     /locus_tag="IVK82_000898"
     CDS             complement(38846..39493)
                     /locus_tag="IVK82_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP6391976.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(39677..40006)
                     /locus_tag="IVK82_000899"
     CDS             complement(39677..40006)
                     /locus_tag="IVK82_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP6391977.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            40183..40911
                     /gene="sfsA"
                     /locus_tag="IVK82_000900"
     CDS             40183..40911
                     /gene="sfsA"
                     /locus_tag="IVK82_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP6391978.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            40927..41940
                     /locus_tag="IVK82_000901"
     CDS             40927..41940
                     /locus_tag="IVK82_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6391979.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            41940..43217
                     /locus_tag="IVK82_000902"
     CDS             41940..43217
                     /locus_tag="IVK82_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP6391980.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            43280..45982
                     /locus_tag="IVK82_000903"
     CDS             43280..45982
                     /locus_tag="IVK82_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6391981.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            46134..46451
                     /locus_tag="IVK82_000904"
     CDS             46134..46451
                     /locus_tag="IVK82_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6391982.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            46481..46801
                     /locus_tag="IVK82_000905"
     CDS             46481..46801
                     /locus_tag="IVK82_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6391983.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            46909..48369
                     /locus_tag="IVK82_000906"
     CDS             46909..48369
                     /locus_tag="IVK82_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6391984.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            48437..48658
                     /locus_tag="IVK82_000907"
     CDS             48437..48658
                     /locus_tag="IVK82_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391985.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            48689..48871
                     /locus_tag="IVK82_000908"
     CDS             48689..48871
                     /locus_tag="IVK82_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP6391986.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            48871..49284
                     /locus_tag="IVK82_000909"
     CDS             48871..49284
                     /locus_tag="IVK82_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP6391987.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            49407..50588
                     /locus_tag="IVK82_000910"
     CDS             49407..50588
                     /locus_tag="IVK82_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6391988.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            50977..51050
                     /locus_tag="IVK82_000911"
     tRNA            50977..51050
                     /locus_tag="IVK82_000911"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51011..51013,aa:Arg,seq:cct)
BASE COUNT        16985 a         8622 c        10920 g        14530 t
ORIGIN      
        1 ccacacctta taaaaatctt tgacaaatct attcgaagga tcgaatttaa accataattg
       61 tgtttttcct tccattaaca tataaagttc acctcaaaaa gagtatacga acaaatgttt
      121 gtattgtaaa tagaaaaaga tgagctaaat taaatgccca tcttctttga tagatattat
      181 aaatctaata attcttgttt ctttttatta aattcttctt ctgtaatgat tccatcatcc
      241 aatagttctt tatattttct tatttctgaa gctgaggaaa ttgttgatgt attggaattt
      301 gtagcagaaa tatttcttct ttttaaatta taaataattt cgtttaaaga atcacttatc
      361 ctatcaacag tatctttatc aactgcaaca ttcaatactt taccaagagt atcaatagtt
      421 aatgtaccca acagcactcc tcttttttta ctaatattat ttaaattgtc taatataata
      481 ctttgtacat tattgcctat cattttttgt tgagctacaa ttaaacgttt attagttaat
      541 gcgtatgcat aattattgtc atgcttagtt gatgagatat aattatgcaa tcctacaaag
      601 cagaagatta cgtattcgtc actgttaagt tgatctgata ttaagttaaa atgatgagtt
      661 gtccattttt tagtcattcc tttaccgtag ccgtatttaa gacaaaattc taccatctct
      721 tcagtggttc tcattctatc attacctata tcatgattgt tttgtgcagt atgatcagat
      781 atgtttaatt ttcttttttc tgatggatcc ttatctaaat cttctaaatt caaagatctt
      841 attattttat aaggagtagt cataccgtaa ccagcttttt tcatacaatt gccgcaaaca
      901 atttgtttat ctttgatttt atatgaagtt aagccaattt tacctccgca ctttgcacat
      961 tgtttactca cattagttcc tcctagatat cagctttcta ctactattaa cattctatca
     1021 atagtgacca tactttgacc atacttttta gaaagaatag aaaattccaa gatatttaat
     1081 aatagaagaa aagcactaac atcatgggtt cagaagaatt gagagcttat caggaaacag
     1141 tataattgaa gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa
     1201 agaggttgtg acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt
     1261 ggaggctacg aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc
     1321 aggatgtttt gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga
     1381 aataattata tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga
     1441 tttttcgttc ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat
     1501 caggtaaata gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta
     1561 caatagtcta tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt
     1621 atgtcaaaga aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac
     1681 aggaaattgc tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc
     1741 tagtgcgcga aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa
     1801 cactcgttac gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg
     1861 tctctgaaga aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg
     1921 gagctaacgg cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc
     1981 caagaggatt aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac
     2041 acgcgatgta tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg
     2101 ttttcaattg tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt
     2161 acgcaaaagg tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg
     2221 atggcggtat gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga
     2281 tcttttactt tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg
     2341 aagcaaatgt acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa
     2401 cttttgttcg gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa
     2461 atgagaaagt cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa
     2521 agatttcttt gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata
     2581 taggacaatt gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc
     2641 aatcacccga aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga
     2701 ttcaattagc aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga
     2761 taactgtagc accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt
     2821 ataatctata tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc
     2881 aagaagcaat caatcatttc atttctacag atatcaacat ccatctgaag tctttctata
     2941 aagaccatgg tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa
     3001 tcatagaagt gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc
     3061 agcaaaactt tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact
     3121 taggagaaga acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg
     3181 aatatgctgt tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc
     3241 cagaaagtga agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa
     3301 gcggcagaat cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc
     3361 aagttgtcca acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata
     3421 tggatccgaa aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa
     3481 gcggggtcat actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa
     3541 gagacactgg aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa
     3601 ctgtacgaaa ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa
     3661 attttcgagg ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc
     3721 ataagaaaaa agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca
     3781 aagaattgat cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt
     3841 tgtcgattgt ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag
     3901 ctactactgg gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta
     3961 ttgatcaaaa tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa
     4021 ctgaattcat ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg
     4081 ataacttcat ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac
     4141 aaatctctca gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga
     4201 tgcacacagc aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa
     4261 acgaattgac aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc
     4321 tgttggaaga tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc
     4381 tagtccataa tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata
     4441 cacttttata gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt
     4501 atcgtaaaaa agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc
     4561 acacagaaat gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat
     4621 attcgattgg cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg
     4681 aagcaaacag gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg
     4741 agaaagtttc tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg
     4801 cagaaaatga ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta
     4861 ttcaccaatc cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg
     4921 aatatcggcg gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta
     4981 aatcaacaag atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt
     5041 cgaaaagtac ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag
     5101 gtgtagcaag aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta
     5161 ttaatatttt tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt
     5221 atcctagcaa gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt
     5281 ggcgaacaag aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg
     5341 agagccaaat tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta
     5401 gttgatttat ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa
     5461 gataaatggg caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca
     5521 cgcttttcaa tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac
     5581 ggtgtaaaag taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa
     5641 gacgcacaac ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa
     5701 tatgtattag cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca
     5761 aaagcagtgc aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg
     5821 cgtaagagac taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt
     5881 agcaaaatga tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta
     5941 tttgaaaatc ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta
     6001 aatgttgggt caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg
     6061 ggacaagaag acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta
     6121 cgtaaagtac ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct
     6181 gaattagcaa aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta
     6241 tttcaataat tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg
     6301 aatttcaatt tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc
     6361 tcgaagctgg tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata
     6421 tcggtgccgc tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag
     6481 ttttaggtgg acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg
     6541 ctgttgcagg gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt
     6601 tgatggatgc ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg
     6661 ctgtatgttt acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag
     6721 cacaaactgt gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta
     6781 tcggtggggg aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa
     6841 aagaagtatg gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat
     6901 tgattgctct aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa
     6961 tgggtggttc atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt
     7021 tgaatgacta ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc
     7081 aaaaaaagat cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta
     7141 cgaacgtatg caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata
     7201 caaaacaaaa gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca
     7261 cccatacatt gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa
     7321 tggcgcgcct gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt
     7381 agctggtgtt ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg
     7441 tgcttcatta gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa
     7501 cattattcgt tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa
     7561 aattactgat gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt
     7621 agggatgttc gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt
     7681 tgtatcaact gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg
     7741 tggtgaagga atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga
     7801 aaaggtaaca acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg
     7861 attaacattg ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct
     7921 tggtttattc gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat
     7981 atagctcggg cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct
     8041 ggagggagaa aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt
     8101 cgtttacggg tctgacagat tacgggcaaa tcatgatagg tgataatggt tttgagtttt
     8161 accatgcgcg agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac
     8221 tagcatcagt catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa
     8281 atggtacata cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat
     8341 atgtagatga agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta
     8401 ttcgtagtat gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt
     8461 tattttttta ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta
     8521 aatcccatat gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga
     8581 cattgattgt tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt
     8641 cgatgggctg tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga
     8701 ttttcggcga atggggacca aaagctgcag gtatggcact tattccatct attttaggcg
     8761 cagtgatttt ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa
     8821 aatagtaaac taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta
     8881 aaataaaaag aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc
     8941 tataatggaa aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa
     9001 aattgcgcaa aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca
     9061 atcttcgtta cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa
     9121 aagcattttt tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt
     9181 ttgaaattat taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag
     9241 aagaactgca ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt
     9301 tagaagaaat catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag
     9361 aagtgaaaga tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg
     9421 catatgatca catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc
     9481 aactagactt ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg
     9541 ctagcggatt gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag
     9601 gtgacttgat cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac
     9661 gtacattcgc tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag
     9721 atgctcaatt agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg
     9781 cagttgctag agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag
     9841 gacatgggat cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc
     9901 aatttgtacc aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg
     9961 gagtacgtat tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt
    10021 caccaaaaga attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag
    10081 tgttcacaga ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt
    10141 taagctgtaa gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga
    10201 tcggcggcag ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat
    10261 ctcgtgctta ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa
    10321 cgcaaaccaa gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg
    10381 gattgcagct tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt
    10441 cactgaatta ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga
    10501 tggcttgaaa gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc
    10561 aatggaaatg caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc
    10621 tgaagtcaat atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga
    10681 agaacttttt gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc
    10741 gggaaatggc attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag
    10801 atgcgatttt caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag
    10861 agtttgttcc tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat
    10921 tggataaagt gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa
    10981 tggatttggt gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag
    11041 cggctgttgc tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac
    11101 gtaaattcgt caacggtgtt ttatccaatg tattgaaaga attagaagct ggggcgtaag
    11161 tcctagcttt tttctatgaa tcatcataaa cctgttataa atcgggaaaa ttctatggta
    11221 gaatgaaaac acaaaaaata tctgggagtg gttagatggc agaattgatt aacggaaaag
    11281 aattagctga aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg aaagataatg
    11341 gtatccatcc cggattagtc gttttattgg taggggaaaa tccagccagc caaatctatg
    11401 taagaaataa agaacgtgta gcaaaagaaa tcggaatcca ttcattggta gagcgttatc
    11461 ctgagacgat ttctgaagac gaactacttg cagaaattga aaaatacaat caagatccta
    11521 gttttcatgg tatattggtc caattaccat tgccaaagca tatagatgaa gaaaagatcc
    11581 tgttagctat ccgccctgaa aaagacgtag acggttttca tccgatgaat ttaggcagac
    11641 tccttgcggg taaaccggac aagattcctt gtactccata cggcatcatg aaaatgtttg
    11701 aagcttattc tatcgatccg gcgggtaaac gagcagtagt tatcggacga agcaatatcg
    11761 tcggtaaacc gatggctcag ctactcttga tggcagatgc aactgttact gttgctcatt
    11821 ctaaaaccga aaatctggct gaactggcac aagaagcaga tattttagtc gtagcaatag
    11881 gacgtggaca ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg atagatgtag
    11941 ggatgaatcg tgatgctaac ggtaagctga tcggtgatgt gaaatttgat gaagtggagc
    12001 ctattgcagg ctggatcact cctgtgccaa agggtgtagg gccaatgact atcacgatgc
    12061 tgatgtatca aaccgtaaaa tctgccgaag cttttggagc aggtgaatag atggcacaag
    12121 aatatttaac tgtcactaca ttaacgaagt atttgaaaag aaaatttgat gccgatcctt
    12181 atttagaacg cgtatattta acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc
    12241 atcaatattt cagcttaaaa gatgaccatg caaaaatatc tgctattatg ttcaaaggag
    12301 cattccaaaa attacgattc cagccaaaag aaggaatgaa agtccttgtc gttgggcgta
    12361 tttcattata tgaagcggga ggttcttatc aaatctatgt agagcacatg gagcctgatg
    12421 gaataggtgc tttatatcaa gaattggaag aacgaaaaga aaagctgatg aaggaaggcc
    12481 tttttcaagg gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg gtccttacca
    12541 gtccaagcgg agcggttatc cgcgatatca tcacgacggt gaaaagaaga tatccaattg
    12601 tccaattggt tttgttccca acagttgtac aaggaaacca agcagcggac aatgttgtca
    12661 ggaatatcca gagagtagaa gagatgggca atttcgatac aatgatcatc ggtcgtgggg
    12721 gtggttcgat tgaggattta tggccgttta atgaagagcg ggttgctcgg gcgatttatc
    12781 aagctaatac gccggtgatt tcatctgtag gacatgaaac agataccacg atcgctgatt
    12841 tagtagcaga tgttcgggct gccactccaa cagcagctgc agaattagct gttcctgttt
    12901 tgtcagaaga gttgatgcgc attcaagaac ggcaagctag attgcagcag gcgtttgtac
    12961 gccagatcca gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat gtatttcggc
    13021 agcctgaaag attgtatgaa ggtcagacca tcaaattaga ccagttgaaa cagcgattat
    13081 atcaagctac acagcaaatt tatcatgcga aagaaaaaca aacgatcgca ttaagccacc
    13141 aattggaaca agtggctccg ctttatcgag ttaaagcagc tcgccaagaa actgcttatc
    13201 tagaaaaacg cttagaagag aaaatccaac aatatatgaa acaacagcag cagcgttttg
    13261 ctcaagctgt tcaatctctt gatttgttga gtccattgaa aatcatggga agaggctaca
    13321 gctatacgac gttaaatgat caagtagtaa aaagtgtcac cgagattcat caaggggatt
    13381 tgctgacgat ccattatcaa gatggaatcg ttaaaggaca agtacaagag atagacaaag
    13441 gagaataaaa atatggaaaa tccaactttt gaagagtcac tgcaagaatt agaaaaaatt
    13501 gtcatgcagt tagaacaagg agacgttcca ttagaatcag ctctagattc gtttaaacga
    13561 ggaatggaat taagcaaaca ttgccaagat acattagcta aagcagaaga gaccttgacg
    13621 aaaatgatga cagaatcaaa tgaagaagtt gattttgacg gaaacgagga aagctgagat
    13681 gagattaact gacttttcag ctattcatct acctgctgta gaaaaagaaa tcttgtcttt
    13741 tttagatgag catacaacgg atagaggact ctatgatgcg atgagttatt cagtgaaagc
    13801 aggcgggaaa agaatacgtc ccttattact tttaacggcc gttgcttcct ttgatgagcc
    13861 tattgatgtt cccgtctatc aagtagcagc tgctttagaa atggtgcata cgtattcctt
    13921 gatccacgat gatttgcctg cgatggacaa tgatgatctt cgtagaggaa aacctaccaa
    13981 tcataaagta ttcggcgaag cattagctat attggccgga gatggtttgc ttacaggagc
    14041 attccagcta atcagtatgg cacacttagg taattctcct aagcttctgc tgcttcagca
    14101 attagctgtt tgtgcgggat cacaaggtat ggttgctggg caagctgcag atatcgaggg
    14161 cgaatcgaaa aagctttctt tagaagaatt agcatttatt catgaaagaa agacagggca
    14221 tttgattcgt tatgccttgc tggctggtgg catattagcg aagcaacctg aagaaatctt
    14281 attgctactt caacgattag ccgaacattt aggtttagct tttcagataa gagatgatct
    14341 actcgatgtg attggtacaa cgaaaacatt agggaaaaca gctggtaaag atgaacgtat
    14401 ggaaaaaaat acttacccgc gtttattagg cctagaaaag acacgtgagg cgttggagat
    14461 tgagctgtta tccgccaaca agatcataga caaattagaa gaggacgttt tttcttttga
    14521 tggcgggctg atgcgccaga tgatcaagca atttgatgtg gagaaatgag ggaaagaatg
    14581 gaaaaagaac gcgtagatgt attagcggtc aaacaaggat tatttgaaac aagagaacaa
    14641 gccaaacgtt cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa
    14701 ccgggagaaa aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa acttccttat
    14761 gtttctcgag ggggattaaa attagaaaaa gcactcaaag catttgatct atctgttgaa
    14821 ggaaaaacat tgttagatat tggtgcatcc actggcggat ttactgatgc agctttgcaa
    14881 aatggcgcaa aaatgagtta tgctttagac gttggctaca atcagctagc ttggaaaata
    14941 agacaagatc ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa
    15001 gactttacct taggaattcc agaagttgct gttatcgatg tttcttttat ttcactaaaa
    15061 ctgatgctgc ctccattaca tgctatttta aaagaagagg gagaagtgat tgctttaatc
    15121 aaaccccaat tcgaagcagg cagagaagct gtagggaaaa acggaattgt tcgtgaccct
    15181 cagacccatc aaaaagtttt agaagacatt ttgtcatttg cggcacaagg tggatatgat
    15241 gtgctggaat taagttattc tccgatcacg gggggagaag gaaatatcga gttcttggct
    15301 cacttgagga aagtacccga aagcggaaca atcaattcag ctattaatat ggcagaagtg
    15361 gtttcaaatg cacacgaaca gtttgaccat aaataaattg taactgtgaa aatcaagtga
    15421 gaagtatgcg gattcttctt tattatacca aggaaataag ctatcattga ttaagaagaa
    15481 tatgctaagg agcgaagacg taatgagaaa aaaagatcgg catcgtttga ttactcgatt
    15541 gttgagtgag caagatatcc gaaagcagga agaatttgtt gaaatcctga aaagcaaagg
    15601 aatatctgtt actcaagcta cgatttccag agatataaag gaactgaaac tgatcaaggt
    15661 accggcttca gatggcggct accgttatag cataccagct gaaacaaacg aagatgtaaa
    15721 tactaaattg gaaaaactat taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt
    15781 tgttattttg aaaacattgc cgggaaatgc ttcggcagca gcaaatttga ttgataaacg
    15841 ttacaagaaa gaattatttt cgattatcaa tgatgacgat aatgttttga tgattacacg
    15901 aactgaagac caggcagttt tattgaagaa agacttcctg cattaccttt aagttgagga
    15961 gcgaatgtga tgctacaaga aatcagtatt actaactttg cgattattcc ggaattgcgg
    16021 ctttcttttc acgaaggaat gaccgcgctg accggagaga ctggtgcggg taaatccatt
    16081 attatcgatg ctttaggtct gttagcagga ggaagaggtt caagcgatta tatacgtcaa
    16141 ggagcagaaa aatgtgtgct tgaagggttg tttgaactac caaaacaaga aggattctct
    16201 gaactgatgg ttgaattagg gatcgaaaca gatgaggaca atctgattgt tcgacgggat
    16261 atgtccttaa ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac attagctaat
    16321 ctgagaagaa tcggcagtta tttagtagat atccaaggac aaaacgaaca tcaggaactg
    16381 ctccagcctg aatcacatct tgctttattg gatcgttttg gagatgtagc tttccaacag
    16441 aagaaaaaga gttatcagca agaatatatc tcttatcgtg agctggaaaa aagggtacga
    16501 aaagtgcagc aaaatgaaaa atcctatgtt cagagaatag atatgctcca tttccagcaa
    16561 gaagaaatag ctgctgctga tttgcaagtc ggagaagaag agaaattaaa agaagaaaga
    16621 gaaaaactta gcaattatca aaaaattgct gatggattag ctgcaggtta cggagcactg
    16681 acgaataatg aacaaaacag tgtagatggt gttggcttgg ccgtttcaga actgcaaggg
    16741 atcgcccatc tagatgtgga gtatgaagca atttatgaaa atatccaaag tgcttactat
    16801 ttgctgcaag atgcaatcgg cgatatgagt cgtcagattg atttattgga gttagatgag
    16861 agtcgtttag aagaagttac gcaacgttta gaattaatcc gacagttgaa acgtaaatac
    16921 ggcgaatcca ttgagtcaat attggcttac tatgacgaaa tcaccgaaga gctagcttct
    16981 tctgattttt cagaaggtca attggacaaa atgaaagagg aactagagca aaaagagctt
    17041 ctacttcagc aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg
    17101 gaaaaatcca ttctgcatga attgaaaagt ctgtatatgg aaaacactga gtttgaggta
    17161 cgtttcttaa aagaagaaaa tagacaattg aatcatgatg gatttgacca aatcgaattt
    17221 tacattacga ccaatccagg agagccactg aagcctttgg tgaaagtcgc ttctggtggc
    17281 gaactgtctc ggatgttgct tgctttgaaa actatttttt cttctgagca aggtgtgaca
    17341 agtattattt ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca ggccatcgca
    17401 gataaaattt ctcaaatatc gaaatactct caagttttgt gcatcactca tctgcctcaa
    17461 gttgctgcag tcgccgacta tcaatattat attgtgaaag cagtgattga tggacgtacg
    17521 caaacttctg tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg
    17581 gctggaagtg agattacaaa actcacactc gaacatgcaa aagaactatt gaaactcgca
    17641 aaaaggacct gagccacctc gcagtggttc aggtcctttt cagcagctga tagctatatt
    17701 ttatttttac atgacactca ttacagcagt caatacaaca gatccaatcg ttaaaatcag
    17761 cattgtccaa atgacaaatt ttgttacttt agcaaaagta cttgtttgtt ttttttcacg
    17821 cattgttttc aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc
    17881 cacaagaggg cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca
    17941 aataaataat gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa
    18001 cttaatgttc attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa
    18061 agaagttaaa attttcataa tattattcag attattcgat acgattgctg aaaaagtctg
    18121 attcatttgt tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc
    18181 caccaataca tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg
    18241 cgctagttct ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct
    18301 tttttggata tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc
    18361 ttccaaagta tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac
    18421 ctgccaaatg aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt
    18481 ctggctaaag actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt
    18541 tttgggcgtc aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact
    18601 tgttttagca aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt
    18661 gaattcttct gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg
    18721 aatttcaaat tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata
    18781 ccagatcgtt tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa
    18841 atatttgatc atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt
    18901 ggaatacatg gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg
    18961 aaacagttgt gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt
    19021 tcatgggcac attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt
    19081 ttggttgcca atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc
    19141 ctgatttggc gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga
    19201 caatttctat cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg
    19261 ttttccaagc atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc
    19321 gtttggtttt tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt
    19381 cttacctatg aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt
    19441 gatttttaaa aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg
    19501 gtagaaagtg ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca
    19561 atgttgatgg gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca
    19621 aaacttcgtg aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt
    19681 ttcggctatc cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg
    19741 gctaaaaaag acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa
    19801 cttgataaac aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca
    19861 aaaggttgcg tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg
    19921 caagattttt ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat
    19981 ttcggactat aagaatagga agaggctgac taatgactga aacatttcaa cactatacag
    20041 tcatgttgaa agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact
    20101 gtacactcgg cggtgccgga catagtgaat atttattaag ccaactaagt tctaaagggc
    20161 atctgtatgc gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt
    20221 cttatgtcga acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac
    20281 gcttgagtga gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc
    20341 ctcagctaga tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc
    20401 gcatggatca gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg
    20461 aattagtaaa gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg
    20521 aaatcgaacg agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca
    20581 ttaaacaagt catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt
    20641 ttcaagctgt gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac
    20701 aggcaatccg gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg
    20761 aagacagaat tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg
    20821 gacttcctgt catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa
    20881 tccttccttc agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta
    20941 ttgcagaaaa aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa
    21001 gattacgaat atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct
    21061 tcttcgaatc aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct
    21121 gctttggaaa aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta
    21181 acagttatgc tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca
    21241 gaaatcaatg ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct
    21301 cgtactgaac ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat
    21361 ttaaggaaag tgaagtaaat gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa
    21421 aatctaaatc cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt
    21481 ggattgttct ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct
    21541 ggtacctcat tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca
    21601 aaaagaggaa cgatttatga tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat
    21661 tcaattaaag cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa
    21721 gaaaagaatt ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa
    21781 gatgcactga atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt
    21841 agctatggga aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa
    21901 gaaggtgtcg ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc
    21961 ttttcttctc attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt
    22021 aaactaggtt tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt
    22081 tatcaaaaag ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct
    22141 gtcgacggac aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca
    22201 ctgatggatc aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa
    22261 gcaaaaaccg gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg
    22321 gaaggattga ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa
    22381 ccaggatcaa ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac
    22441 gagaatgaaa ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat
    22501 gactttggtg aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc
    22561 gggatggtca tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg
    22621 ggatttggac aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca
    22681 ttaaatattg tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc
    22741 caaatgatga aagctttcac ttctattgcg aataacggta caatgattca acctcgatat
    22801 atcagcaaag tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta
    22861 gggcaacctt tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc
    22921 gaaagcgaaa actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg
    22981 gcaaaaaacg gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca
    23041 gcctacctgt attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat
    23101 ctaacaatga aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca
    23161 ttgatgaaac gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa
    23221 acagaaaaag tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat
    23281 gctcaaaaaa gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa
    23341 tctactgcca acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt
    23401 gataagctga tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa
    23461 atcctcggta ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct
    23521 ccatatgaaa aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa
    23581 taggagagaa aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc
    23641 aatgacaatt gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca
    23701 ggcgattcgt gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg
    23761 cggcttagtg tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa
    23821 acagctaact cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg
    23881 ctttcttgat gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca
    23941 gaaattgatt ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg
    24001 ttatccaggt gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat
    24061 tttcgctctc ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga
    24121 tggattagtt gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca
    24181 tcagcatcag tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt
    24241 cttcgtctac aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt
    24301 gggcggactt ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat
    24361 tggtttgatc tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct
    24421 tacaggaaaa cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg
    24481 gtcggagtgg aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac
    24541 attatggatc gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata
    24601 agaaagtgct tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg
    24661 aattgggcgc acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac
    24721 aagagctgct ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg
    24781 atgaagaatt ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag
    24841 caaaagccca agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag
    24901 cggaatgccc aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga
    24961 caggactact actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg
    25021 ggtatccagc aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat
    25081 tgtctagctt ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca
    25141 atatctatga agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat
    25201 ggaaccttca aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag
    25261 aactacaaga attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag
    25321 tgttggaaga cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa
    25381 tggaaatatc tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta
    25441 tttctgtagc aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt
    25501 tccatggcgt accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata
    25561 atgattcaaa agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt
    25621 ctaaggtcgt gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc
    25681 cttcattaaa aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg
    25741 atgctggaaa aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag
    25801 ttcctttagc attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg
    25861 ccagctggga tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga
    25921 accgattaaa atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc
    25981 ggaggaacag ggggacatat ttatccagct cttgcctttg tcaattatgt aaagactcaa
    26041 gaaccgaatg ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc
    26101 cctgatacag gaatgccttt ccataccttg gagattcaag gatttaaacg aaaaatttcc
    26161 atgcataaca tcaagacggt ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc
    26221 ctcagtgatt ttcaaccaga tatcgttatc ggtactggag gatatgtttc cggagcggta
    26281 gtgtacgctg cagccaaaat gggtattcca acagttatcc atgaacaaaa cagtgtacca
    26341 ggtgttacca ataaattttt aagccgatat gtggatcgga tcgctctttc atttaaagat
    26401 gcggcttcct tttttcctga aaacaaagca gttcttgtag gtaatcctcg agcacaagaa
    26461 gtagcagata ctaaaaaatc agatattttg aaaacatttg atttggatcc agaaaagaaa
    26521 acagtcttga tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt
    26581 tttttaagtg aatatattcc ttcttcttac caagttttgt atgcttcagg agatagatat
    26641 tatgaagaga tcctaaataa aataaatcct gaaatacaaa atattagtgt ccagccttat
    26701 atcaaaaata tggcagaggt catggcttcc agtgatctgc tggtggggcg tgctggtgca
    26761 acttcgatcg cagaattcac agctctgggg ttaccggcag tgttggttcc cagcccatat
    26821 gttacgaatg atcatcagac taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa
    26881 atgattgcag ataatgaact aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg
    26941 ggtgatgaag aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg aatccctgat
    27001 gcttcaaaac gtctttatga cttagtaaaa caaataatca aaaaataaag gcggtggtct
    27061 tagtgagtaa gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt
    27121 tgactccttg gcaaaaggct aaccgaaaat atctggcaga acaagagatg aaaaaccaag
    27181 agccaaaaaa agaagacata acattagata cacccctttc agattctgaa gaagaaaaaa
    27241 cggttcaaga ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag
    27301 agatcaagca aaagaaaaac ggtggaccat ataacggttc ttttttgaac cggctgccca
    27361 atttaaaaag tcaacggcac aaagtactat ttcgtcggtt agctttgatc atcactgtat
    27421 tgggtatacc gctgattttt ttgatctatt atgtttctcc ttatagcaaa ttacaagcag
    27481 ttgcagtatc aggcaataag acagtcaaca gccaagaaat catctctgat acaaaattgt
    27541 cattaggaga aaatgtttgg ggacagtatt ttcatcgatc tacttatatc gatcgcctta
    27601 aaaaagcaca gccaagaatc gaaacagcaa atatacattt caaagggatg aatgaattcg
    27661 atttggatgt aacagaatat aaagagattg ctttgatcgc aaaaaacaat caatactacc
    27721 cagtgattga aaatggaaca gtattagatg aaaaagtagc aaatcctaca aaaaatctcc
    27781 ctatcttgga agagttcaaa gactctgcaa aaatcaaaga attaacaaag caatacaatc
    27841 agttatcatc agaattacaa aaagcaattt ctgaaatcaa gtacacgcca agagcaagca
    27901 ataaaaatct gatccaattg aatatgaatg acggcaatca agttattgta aatatcaata
    27961 atcttgcgaa ccaaatgaaa tattattcgc aagtagccaa agatatggat gaaaaaggtg
    28021 tgatcgacat ggaagttggt atcttctcgc atccttacgg tagcactcaa gaaacggaag
    28081 aatcgggcca atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat
    28141 caagtgaaga ggcagaaacg caaaaagcac aaacacctgg atcgtctagc acaaacggat
    28201 catctgattc aaatgattca agtgaaacaa gtcaaataga agataatcca agtacaactc
    28261 cgtcaagttt aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt actttctaaa
    28321 aaccagtaat ttatggtaga attggaagca gtgaatatat ctattaataa gtatatttta
    28381 aaacggacgc tagttgttcg cttgtatatg gaatagtaag taggagggaa tcccattcat
    28441 ggcaaaaaca ggaatgtatg taggccttga tattggaacg acatctgtta aagttgtcgt
    28501 ggctgaatat atcgacagcc aaatgaatat tattggagta ggaaacgcaa aatcagaagg
    28561 gattaaccga ggcattatcg ttgatatcga taaaacggtc caagcgatac aacgagcagt
    28621 acgacaagca gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc
    28681 aaacatgctt gaagtagaaa attgccaggg aatgattgca gttaacggag attcaaagga
    28741 aatcaccgat gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc
    28801 tgaacgtcaa atcgtgtcaa tattgccaca agattttact gtcgatgggt tcgagggaat
    28861 caaagatcct cgtggaatga tcggcgtaag attagaaatg tacggtctgt tgtttacagg
    28921 acctaaaacg atcatccata acatacgtaa atgcgtggaa aatgccggat taatcgtcaa
    28981 cgaaatggtg attactcctt tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga
    29041 ttttggtacg atcgtcattg atatgggtgg cggccaaaca acaacggctg ttatgcatga
    29101 taagcaattg aagtttacca atcttgacca agaaggtggc gaatttgtca ctaaagatat
    29161 ctctattgta ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa actacgggga
    29221 tgcataccca gaacggacat caccagatga agaatttcca gtagatgtga ttggtcagtc
    29281 agaaccagtg aaagtcgatg aacgctactt gtcagaaatt atttcagctc gaatggaaca
    29341 gattttcaac aaagcaaaag atgcattgga ccaaatcgaa gcattagagt tgccaggtgg
    29401 cgtcgtattg acaggcggag ctgctagtct tcctggtgtt gtggacttgg cacaagaaat
    29461 cttcggcgta aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa acccggtatt
    29521 tacaaatgta ataagtatcg tggattattc agcaaacttg agtgaagtgt atcaattggc
    29581 aaaaattgct gttacgggtg aaacctcagc agctcgtcaa atggtagttg aacaagaaac
    29641 aacaaacaca tatgagagct atgaagcacc tgaagaaaca atctacgatg agccggaacc
    29701 aaaagagtcc ggagaaaatg taaaaaataa aatcaagggt ttctttacaa atatttttga
    29761 ttaattcatc ctgggaggaa taagatatgg aattctcaat tgataataat atcaatgacg
    29821 gcgcagtaat caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga
    29881 ttgaagaaaa cgttaaaggt gttgaattca ttacagccaa cacagacgtg caagcattaa
    29941 aaaattcaaa agctgaaaca gttatccaat taggccccaa atatactcgt ggtttaggtg
    30001 ctggttctca gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg
    30061 aagctttaga cggtgcagat atgattttca tcactgcagg aatgggtgga ggaaccggta
    30121 caggtgctgc accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg actgttggtg
    30181 tcgtgacacg tccattcact tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta
    30241 tcgctcgttt gaaagaaaat gtggatacat tattaattat ctcaaataac cgtttattag
    30301 aagttgtaga taaaaagaca ccgatgcttg aagctttccg tgaagcagat aatgttttac
    30361 gacaaggtgt acaaggtatc tctgatttga tcacagcgcc aggttacgtt aaccttgact
    30421 ttgctgacgt gaaaacagtg atggaaaacc aaggtacagc attgatgggg atcggtgtgg
    30481 ctagtggaga agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg
    30541 aaacatctat tgatggagct gaacaagtac ttcttaacat cacaggcggt ttggatatga
    30601 cattgttcga agcacaagat gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa
    30661 atatcattct aggtacatct atcaatgaag aaatgggcga tgaaatccgt gtgacggtca
    30721 tcgcaacagg tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca gctagacaag
    30781 cacaaatgca atcacctgct caaaagactg tattagatat ggatcaagct aaaccaactt
    30841 cttcagaaga agaaaacagt tttggcgatt gggatatcag acgtgaacaa aatgttcgtc
    30901 ctagagttga tgattccaat tttgacaata tcgaaaaaaa agaatttgat acattcaacc
    30961 gcgaagaaac aaaatctcat ggcgatgatg aattaaatac gccaccgttt ttccgtcgta
    31021 aaaaataaag gagaggaaga gcacatgatt gctgacaact tgcagaaaat caagcaagaa
    31081 attcaggatt cgtgtgctct tgttcatcgt gatccgaaag aagttacatt agtagctgta
    31141 acaaaatctg ttgaatccgc tcaagctgaa gaattggtta gagctggcgt tatgaattgt
    31201 gcagaaaacc gagtagataa attattagaa aagaaacaag atttatttga ttttcctgaa
    31261 ataaagtggc atttaattgg taatttacag cgcagaaagg taaaattgat tgtaaatgaa
    31321 attgattact ttcatgcgtt ggacagttta agattagcag aagaaatcca gaagcggtta
    31381 gagaaagaat tatcctgttt tattgaagta aatgtttctg gtgaggaatc aaaacatgga
    31441 atttcaccag atgagctttt accgtttgtg gaatctttag ctgcctgcac aaaaatcaag
    31501 gttgttgggt tgatgactat ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg
    31561 tttggtacgc tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca
    31621 tgtagtgaat taagtatggg gatgagtcaa gactttcaaa ttgcgattga agaaggtgca
    31681 acatttatcc gtgtaggaac agcgctattc aaagacgaat aaaggaggaa caaaaatgcc
    31741 gcttttaaac agaggagcaa ttgctaattt tttcggatta gctgatgagg aagagtacga
    31801 ataccaagat tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac
    31861 acagaatcaa tcaactcatt ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc
    31921 gacttcacgt cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa aagtaattga
    31981 actacatcaa tcacctacaa aaactgtagc gaaagaaaga acagcttcaa gaacctctac
    32041 acaacctaaa gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag cttattctga
    32101 agctatggcg attgctaaac gaatcattgc tggagaagca accttagtga attttcattt
    32161 agtagaagaa aaacaagcga gaagaatcgt tgattttcta acaggaacag tttatgcact
    32221 agatggagat atcaaacgtg ttggagatga gatttttctt tgtacaccga ctgggatgga
    32281 gattgacagt tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc tgtaagagga
    32341 ggaactacga tttatactat tgcactatta cttcatttac ttcaacgggc agcctatttc
    32401 tatagcatta tcttgatggt atatgcattg ctctcttggt ttccaggcgg gtatcaatca
    32461 gggctaggac gattattagc taagatctgc gaaccgtata tcagtttgtt cgatcgtctg
    32521 cctttgagga ttggaccctt ggatttcaca attttagggg caatcctgat cttgaactta
    32581 gccgtacaag gtttagcaca gatcgttata tggataactg tccatttagt ttattgattg
    32641 aagcaacaaa atctttattc tcagaataga taggagggga aaattttgga tgcaaatgta
    32701 taccagcact ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga ttggattgaa
    32761 caggttcaag gacaatatgc accctatctg accgaatttc ttgacccaag acaatcctat
    32821 attttagaaa cactgattcg acaaagcagt gatttgcgtt tcatgtttta tggcggatat
    32881 gaacaggcag aaagaaaaag atgtttgatt ttccccgatt attacgaacc tgtagaggaa
    32941 gattttgaaa cagcattgta tgaaatccat tatcctagca aatttgccag cctaagtcat
    33001 gggaaaatcc ttggaacttt agtgggaacg gggatcaaac gctcttactt tggcgatatc
    33061 atctcggacg gtgatcgctg gcaagtattt attgcaaatg aggtggatca ttttgttgct
    33121 gcacaagtaa caaagattgg aaaggttgct gtacgcttgg aaccaatcaa atacacagaa
    33181 atgattatgc cgaaagatag ctggacacaa gaaagaggaa cagttagttc catgcgacta
    33241 gacagtgtga tctctgagat ttataatatt tccagacagc ggtccaagca gctgatcgaa
    33301 gctggtaaag taaaagtgaa ctggacagaa aatacaagac ctgattttgt acttgaacta
    33361 ttagatatta tttcagtcag aggttatggc agagtccaga tccaagaact tgaggggaag
    33421 acgaaaaaag aaaagtacag ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg
    33481 gtttactcat ggctttaact ccattagata tccaaaataa gacattcccg acaaaaatgc
    33541 ggggatataa tcaagacgaa gtagatgatt tcttagattt agttgttcgt gactatgagg
    33601 aattgactca aagaaatcga gaattagaaa aagcagtcaa acattctgaa gaaaaacttg
    33661 agtacttcaa tgaattaaaa gatgctttga accaatcaat catcgtagct caagatacgg
    33721 ctgataaagt aaaaacaagt gcaagcaagg aatccgaagt gatcgttact tctgcacaaa
    33781 acaaagcaga tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta acaactgatg
    33841 cagaagaaaa agcaagaaaa atcttgacag atgcaacaga aaaagcaaga cagcttgcga
    33901 cagaaactga agatcttaag aaaaaaacac gagtattcca tcaacgtatc agcttgatgc
    33961 ttgagtctca attagaacaa gtgaaaagtc cggaatggga tgaaatttta caaccattct
    34021 ccagctacgt gactgacagc catgaagtga tcaaagaagt tttagcaaaa cagcttgaca
    34081 atgaaaatga tactgatgta aactcagaca caggagtcat agaagcacct atgacggcat
    34141 ttgatacaca ggctattgat atctcgatcg tgcctggtga aaatgaaaac gagtagaaca
    34201 gaaaagcact ttgttattct taaagcgagc cgatgagagt ggaagttcgg taagacactg
    34261 catggtgaaa gatcctctac ttgagacctg cttttccgaa attagagtaa gaagcagcgg
    34321 ctgacctcgt tattggtcac agagagtttg cactagatag atgcaactaa atttgggtgg
    34381 taccacggca tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg aaaacaaata
    34441 aaggagtgta tggttgaatg aaaatgaaag aaaccttgca attaggaaaa acaaaattcc
    34501 ctatgcgtgg taatttgcca aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc
    34561 gaatctacca aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt cttcatgatg
    34621 gacctccata tgccaatggt aatatccatt taggacattc attgaataaa atcagtaaag
    34681 acattatcat tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt ccaggatggg
    34741 atacacatgg attgccgatt gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa
    34801 tgtccatggc agaatacttg gaaaaatgtc gtgaatatgc actttcccaa gtagataagc
    34861 aacgagcaga tttcaaacgc ctaggagttg caggagattg ggaacatcct tatgttacat
    34921 tagatccttc ttatgaagct gcacaagtac gcgtatttgg taaaatggct gaaaaaggct
    34981 atatttacaa aggattgaaa ccaatttact ggtctccgtc aagtgagtct tctttagcgg
    35041 aagctgaaat tgaatataaa gatgtaaaat ctccatctat ttttgtagct ttcaatgtca
    35101 aagacggcaa agatattctt gatgaagata cagcttttgt catttggacg acaacaccat
    35161 ggacattgcc agcaaaccaa ggtattgctg taaatccaaa atttacgtat gtattggtgg
    35221 aagccgatgg ccgtaagttt gtcgtagcga aagatttgct agaaacagtc caatcagaaa
    35281 tcggttggga aaatgtaaca gttctaaaag aatttgctgg acaagaaatg gaatatatga
    35341 ctgcaagtca tccattctat gaacgtgaat cacttgtgat tttaggcgat catgttactt
    35401 tagacgctgg tactggatta gtccatactg ctccaggaca tggggaagat gactatatcg
    35461 ctggtagcaa atataaacta tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag
    35521 aagaagcacc tggatttgaa ggtatctttt atgacaaagc aaatcctatg atcactgatc
    35581 tattaaaaga aaaaggagct ttgctgaagc tagatttctt tactcatagt tatcctcacg
    35641 attggcggac gaaaaagcct gtgatctatc gggcaactcc acaatggttt gcatctatcg
    35701 ataaattccg tcagaatatt ttggatgaag tagaaaaagt agagtgggtt atcccatggg
    35761 gtaaaacacg tttgtataat atgatccgtg accgcggcga ttgggtaatt tcccgtcagc
    35821 gtgcatgggg agtaccactt ccgattttct atgctgaaaa tggagaagca atcatcacac
    35881 cagaaacaat cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt atctggtttg
    35941 aaaaagaagc gaaagaactt cttcctgaag gatttaccca cccaggttct cctaatggcg
    36001 aatttacaaa agaaaaagat atcatggatg tctggtttga ttctggttct tctcatgaag
    36061 ctgttttacg tcagcgacca gaattatcat tcccagcaga tatgtattta gaaggatcag
    36121 accaatatcg tggctggttc aactcaagta tcacgactag tgtggctatc aatggtgtag
    36181 caccatacaa atcagttatt tctcaaggat ttgctttaga tggtgaaggc cgtaagatga
    36241 gtaaatcttt aggaaacatc attgcaccag aaaaagtgat caaacaattt ggtgcagata
    36301 tccttcgctt atgggtatct agtgtagatt atgaagcaga tgttcgtgtt tctatggata
    36361 tcttaagtca agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt ttcttattag
    36421 cgaatacaga agatttcgat cctaaaaaag atactgtttc gtacaatgat ttacgttctg
    36481 tagacaaata tatgaccgtt cgattgaacc aagtaatcaa agaaatccga gaagaagggt
    36541 acgacaaata taacttcatg catatttacc gtacagttat gaacttcttg acggttgatc
    36601 tatcgtcatt ctacctggat tttgctaaag atgtagttta tattgaagca gaagatgatt
    36661 atcaacgtcg ttgcatgcaa actgttttct atcaaacagc tgtagctttg acaaaattat
    36721 tgactcctat cattccgcat acagcagaag aaatctggtc attcctgaaa gaagaagagg
    36781 agtacgttca gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa gaagaattga
    36841 tggatacctg gactgctttc atggacttcc gtgataatgt cttaaaagca ttggaagaag
    36901 caagaaacag caaattgatt ggtaaatctt tggaagcaaa actaactgtt tatccaaaac
    36961 aacaagttcg tgaaatgtta aaagctttgg atgcagatat tgcacaatta ttgatcgttt
    37021 caccagacta ctttgaagtt atggcagcag atgaacaagt gccagataac gctatggtat
    37081 ttgatgatgt tgctattaca gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc
    37141 aagttcgtaa agatgtagga gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa
    37201 aaatcgttga agacttctat cctgaagctg tagctgaagg attcgaagaa aagtaaaagg
    37261 gaaaaggggg tgtgacatgt tctttgtcac accccctttt gttatcaatc gtttaatttt
    37321 ccacgttcac gataccagtt atctggttgc cagatccatt cgaatccgtc tttttcaagt
    37381 agttcgaaag ctgctttagg tcccattgat ccagctttgt agttgggaaa atccggttgt
    37441 gtttggtccc aagcttttcg aatgacatcc acgatatgcc aagattgagc gacttcttcc
    37501 caatgagaga agttcgttcc atcaccattc aacgcatcta atagtaattt ttcgtaagct
    37561 tctgggctat tttcaaccat ttctgcacta tttctaaatt ccaatttgat tggttccgtt
    37621 tcaaatcctt gccctacttc tttcccattc aaggataaag aaaatccttc agttggctga
    37681 atatagatcg ttagaacatt aggtggaagt gtcgtatcgt cacatggttc atcgacagat
    37741 gttttgaata cattaacagg aacttgtttg aatacgatgt tgatccgagt ccctttttca
    37801 gtcaaacgtt tgcctgtacg tacatagaaa ggaacacctg accaacggaa gttatcgata
    37861 gtgaatttgc ctgcgacgaa tgtttcagtc tctgattgag catccacttt gtcttcttcg
    37921 cgatagccct tgaattgtac accatccaat tcaccttgag catattgccc gcgaacgaag
    37981 ttttctaaca cttcttcttc gttataaatg cggatagctt tcaatgcttt gatcttttct
    38041 gtacggattt ctttttcgga aaatgctact ggcggttcca ttgcaagtaa tgctacaacc
    38101 tgtaaaatat gattttgcac catgtcttta agtgcgccac tatgatcgta gtagcccccg
    38161 cgatcttcta caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat atagcgattg
    38221 ttccattgag cctcaaaaat attattggca aaacggatag ctgagatgtt ttggatcatt
    38281 tcttttccta aataatgatc aatcctgaaa atatcttgtt ctgggaatac ttctctgatt
    38341 tgctggttca attcaaaagc agactggtaa tcagaaccga acggtttttc tatgatcaaa
    38401 cgattgaagc cattttttgt cataatattt tgtgatttca aatgagaaac gatcgttcca
    38461 aaaaattgag gtgccatagc caaataatag acacggttac cgtttaattg atagtgtcca
    38521 tctaatcggt cagacaattc ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg
    38581 gattgatagt aaaaatggct tgcaaaggta gttgcttctt catctgaagg attcaatgca
    38641 cggattgttt ctttgacaac ttcacggtag tgctcatcgc tccagggacg gcgagcggtt
    38701 ccgattacag caaaatgctc gcctaaattt ccttttctgt aaaggcggaa caaagacggg
    38761 tacaatttac gctgggctag gtcacctgta ccgccgaata tcgtaaacaa tacatttttt
    38821 tcattagaca tgtggataca ctcctttata ttttatcgac aaaaatcgtg ctggccgcct
    38881 taaagctgac ggggattttc tttctatcat tttggatagt gatcggtcct tcataagctg
    38941 cgatgtcaac gatctcatat tcttcgttga tattcaggtc aatagtgact aagtaatcca
    39001 acaattcttt ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca ggataggact
    39061 ccaatgtttg tcggcgttct tcatggacaa cttcctcttt tttaggaatc gatcctccat
    39121 gaggacaatg ttcaggatga ttcaaatagt tttctaaatg atctgcaaga tgttccgaag
    39181 tcacatgttc caatacttca gcatcatcat ggacttcatt ccagctatag tctaaatgtt
    39241 caactaaaaa aacttcccat aagcgatgtt tgcgaatcag actgctagct ttttgcagtc
    39301 cgcttgcagt caattgtaca ccttgataag gggaatgttc tacaagctct tctttcacaa
    39361 gtttagaaat catttcactg acagatgcag ctgagacatc tagactagat acgatttgtt
    39421 tattgttgac tttcgttgta tctccaccta gttctaaaat gattttcaaa taatcttctc
    39481 gatttggtgt catattcgat catcctttca tggtaaaaaa ccatcattag taagtgtaac
    39541 aaaaaaaacg ttaattgact atttttctaa ataaaaaaca aaggattttt agacgttttt
    39601 acaacaaaaa acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat
    39661 atacattggg agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt
    39721 cataaagagt ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga
    39781 ctaaacgata ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa
    39841 acgtgtaatc agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg
    39901 taccttgata ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa
    39961 taaaacttga gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca
    40021 aatcactcct ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt
    40081 accgaataaa agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac
    40141 aaattatgat aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg
    40201 gaaatagctt tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac
    40261 ggagaaataa ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca
    40321 ggcgctgaag tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg
    40381 attgctgtaa aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg
    40441 gcaattgatg gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg
    40501 gtatacaagc gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg
    40561 gtcggacaaa aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt
    40621 gcttttcctg atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc
    40681 gcacaagatg gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg
    40741 gcaacgatcc atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa
    40801 agcggagttc aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag
    40861 caggccattc catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt
    40921 aaaataatga tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa
    40981 gcagctttaa gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa
    41041 gcacaggaat ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact
    41101 tttttcaata ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt
    41161 agtcaagcta agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt
    41221 tcgacgccaa aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt
    41281 tttgaagcag caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca
    41341 ttaaaagacg agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac
    41401 caagtattag atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg
    41461 atggatttag gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa
    41521 gtccattatt tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta
    41581 cgttatgaga attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg
    41641 tcagagattt attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa
    41701 gcacaatttc atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa
    41761 aatccaatgg cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca
    41821 gcttggggat tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt
    41881 tatgatttaa gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa
    41941 tgaacgaatt tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt
    42001 ttatctcacc ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta
    42061 tagtagcggt ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac
    42121 taaacgtgga accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag
    42181 ctattcaagt agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat
    42241 ctttggtggg cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa
    42301 tcttgattgg tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac
    42361 atcaagtgaa aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc
    42421 aaatcattga tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag
    42481 cgacagcaga tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa
    42541 ttgacgtgac agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac
    42601 cgatacgaaa gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc
    42661 tgattttctt taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag
    42721 gtgtaccggt aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag
    42781 aacaattcaa aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt
    42841 tagatattac aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt
    42901 atttgcatcg ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg
    42961 tacaagataa taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg
    43021 aagagatttt tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag
    43081 ctcctgcagt aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat
    43141 ataacaaaga aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag
    43201 gagcaaggcg caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg
    43261 ttgccagagg aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga
    43321 aaaccaacaa ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa
    43381 tgcaagccga tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg
    43441 accagtcgtt tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc
    43501 ttctacgcaa gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat
    43561 acagcaggca aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg
    43621 agaaactggt acagggaagt cgatgtttgc tgaatgtatg tatcattttg caatcgattc
    43681 agagatgctt tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa
    43741 tccgcaactg ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc
    43801 tcaagatagt cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat
    43861 ccaccgttta ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta
    43921 ccgtccgctt ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac
    43981 gactgaatct tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac
    44041 cttgccgaat cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat
    44101 caaacaagaa gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc
    44161 cttcatgctt tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt
    44221 ctgtgcaaaa tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa
    44281 agaagaactt tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg
    44341 tctcgaccga ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga
    44401 tgttgtctgg tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa
    44461 agtcacttct ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa
    44521 agatgtggat gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa
    44581 agatcttttg ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa
    44641 acggttagac cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag
    44701 tacattggat cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg
    44761 gaaaaatttt aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga
    44821 agaacaagtt gagattcctt ttgatgaaat cggctttatt agtatgtttc tttctatcaa
    44881 tctaggagaa agtgagcctg ttaaagaaaa caaagtagat gttctcatat tgatgcacgg
    44941 cagacagact gcttcaagca tgttggagat ggcacaagaa ctattaggaa caaaagtagg
    45001 gaaagctttt aatatgccac tggttatgga agttcaaaca atgtatgaag aagtgttaca
    45061 atatgtcaaa aactatcaag agcatttgac gaatggccta ctcttgttaa cagatatggg
    45121 atcattaaac accttcagtg atttgattta tgaagagact ggcattcgga cgaaagctat
    45181 cacgatgaca agtaccatga ttgtgataga agcgatacgg atggcaagtg ttggaagaag
    45241 cttagaagat atttatcaaa atatccagct ttcctttgaa tcgatcgtac gtgatcagtt
    45301 caaagcggaa aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga catgctttac
    45361 tggtgaagga gtagctgcca aattatatca gcgtattatg ccagtgatta atcaaaataa
    45421 ggtagaactg atccagatgc agtttttaga acgtgaatca ttcaaaaaac acatcgatgc
    45481 cttattggaa gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa
    45541 tatcccattt ttttcagcct atgacgtttt tgatgatgaa aaactaaata ttttaaagcg
    45601 tatagctgat gatgagatcc cgacagctaa aattgtcgat tcacttgctg gatcattaag
    45661 cgatgtcttt tctattgatg aactgatccg ttcattgcaa aagacagttc agcagatcca
    45721 gaccgatttg tcgattatcg tggaacctgg agtagatact ggaatcgtta tccatttagc
    45781 cttcatgtta gacggattga tcaaacaaga aagccatcgg caatttgaga aactgtctat
    45841 tttctcaaaa atttatcgtt tggaaatgga tatcgttcgg gcaaacttga tggcactaga
    45901 acgaaaatat caggtaaaaa ttccagaaga tgatatcgca tttcttacac aaatgtttct
    45961 tgaaaataaa ataaaaaaat gaacacacta atcatactgt ttacacacta aacatctgtg
    46021 tgcgcactat aaaaaagagc taaaaagcat gaaaacgcta tttcagctct tttttttatt
    46081 ggcatgcttc ttgcgttata taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa
    46141 aaaaaacaat catgttagtt tgttcagcag ggatgagtac gagtttattg gtcacaaaaa
    46201 tgcaaaaagc agcagaagaa aaagggatgg aagccgatat ttttgcggtc tctgcctcag
    46261 atgcagataa taatttagaa tctaagaatg tcgatgtatt attattaggt cctcaagtac
    46321 gtttcatgaa agctcaattt gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca
    46381 tcaatatgca agactatggc atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc
    46441 taatgaaata aatcaagtat gcagttgggg gtataacaac gtggaagatc aacaaaatct
    46501 agaagccatc atggggttaa tcatgtttgg cggtaatgca aagagcgatg caatggaagc
    46561 gattgcggct gctaaaatcg gtgattttga gttagcggat aggaaaatcg ctgatgcaga
    46621 agaatcactt atacaagcgc atcatgcaca aacagacatg ttgacacaag aagcacaagg
    46681 aaatcatatg caagtgacat tattgactgt tcacagtcaa gatcacttaa tgacatctat
    46741 tgcgtttact gatttagcaa aagaaatcat cgaactttat cgtcgcgtat acagcgaata
    46801 aatacaactt cttttttgaa cggaagaaga aaaatttttg aacggatttt ttattttgtt
    46861 ataaggagaa ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta
    46921 tcaagttgga tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat
    46981 ttagttgctt tgcgtgatgc ttttatcggc acactgcccg caacaatggc gggatcagtc
    47041 gctgtaatga tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct
    47101 gacagtttag cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat
    47161 ggttttgtct ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg
    47221 tataatcttg cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct
    47281 accttgattc aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt
    47341 ccttcaaatg tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc
    47401 acactgtcag ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat
    47461 tttacagtta tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc
    47521 aacattacga tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc
    47581 attttaccag cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt
    47641 gtagctgatg ggcaactgat cattgatttg gtgcaaaaat atattgcaga accattccta
    47701 ggattatctc aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg
    47761 ttctttggta ttcatggacc aaatgtattg gcaccagtgc tagagggaat ctggggacaa
    47821 gcacaactaa tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca
    47881 gccgtgttag atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat
    47941 gcttttgcat ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt
    48001 ttctctaaac gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc
    48061 aatatcaacg aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc
    48121 ccattcttga ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc
    48181 ctagtcgctc cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc
    48241 ttagcaacag gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact
    48301 ttcttgattt gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa
    48361 caacaataag atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt
    48421 tctaggagga actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga
    48481 attgttagaa ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat
    48541 caagctgttc tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga
    48601 actaatcaaa gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa
    48661 aatggtttaa aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat
    48721 tggttctttg atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag
    48781 tatggtcaca ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa
    48841 aagagaagaa gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca
    48901 gcatcgtttt tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc
    48961 ggcaaatcag taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa
    49021 aataaccggg ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt
    49081 acatcgacaa cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg
    49141 acctgtgagg ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat
    49201 gatatgcttt taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta
    49261 gatatgatcg aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa
    49321 tgaagtaagc tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta
    49381 gcttttttgt aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt
    49441 cgattgagga aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa
    49501 ggcgagaacg ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg
    49561 aggatcggct aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag
    49621 agtacctaat ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg
    49681 ggatcaatca gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat
    49741 cttatctggc gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg
    49801 ataaaatcag ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa
    49861 aaataaagcg tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga
    49921 tgccggaaaa ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca
    49981 agcaaaaagg caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat
    50041 atatcgttta tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc
    50101 atgatgcttt ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat
    50161 ttgaatattc atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg
    50221 cttctgtaat gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg
    50281 aaaattatcc atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt
    50341 atgcgaaatg gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct
    50401 caaaagtaga acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc
    50461 tattattagg gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa
    50521 aagaatatga agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact
    50581 atttttgata aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt
    50641 ggcatttgaa ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt
    50701 ttcttttgtt ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc
    50761 ccgatttttt ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt
    50821 cattggaaag gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga
    50881 ctagtggaaa cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt
    50941 aaaaaacaaa caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga
    51001 gcaatcgcct cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg tataaat
//
LOCUS       DACCCS010000015        50360 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.015, whole genome shotgun sequence.
ACCESSION   DACCCS010000015 DACCCS010000000
VERSION     DACCCS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.015"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(273..1529)
                     /locus_tag="IVK82_000912"
     CDS             complement(273..1529)
                     /locus_tag="IVK82_000912"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP6391989.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            2109..3410
                     /locus_tag="IVK82_000913"
     CDS             2109..3410
                     /locus_tag="IVK82_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP6391990.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(3479..4078)
                     /locus_tag="IVK82_000914"
     CDS             complement(3479..4078)
                     /locus_tag="IVK82_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6391991.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            4332..5213
                     /locus_tag="IVK82_000915"
     CDS             4332..5213
                     /locus_tag="IVK82_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6391992.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            5226..6836
                     /locus_tag="IVK82_000916"
     CDS             5226..6836
                     /locus_tag="IVK82_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP6391993.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(6913..7572)
                     /locus_tag="IVK82_000917"
     CDS             complement(6913..7572)
                     /locus_tag="IVK82_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6391994.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(7855..8520)
                     /locus_tag="IVK82_000918"
     CDS             complement(7855..8520)
                     /locus_tag="IVK82_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6391995.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(8667..9272)
                     /gene="dhaL"
                     /locus_tag="IVK82_000919"
     CDS             complement(8667..9272)
                     /gene="dhaL"
                     /locus_tag="IVK82_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP6391996.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(9300..10283)
                     /gene="dhaK"
                     /locus_tag="IVK82_000920"
     CDS             complement(9300..10283)
                     /gene="dhaK"
                     /locus_tag="IVK82_000920"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP6391997.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(10285..10680)
                     /gene="dhaM"
                     /locus_tag="IVK82_000921"
     CDS             complement(10285..10680)
                     /gene="dhaM"
                     /locus_tag="IVK82_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP6391998.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            10834..11154
                     /locus_tag="IVK82_000922"
     CDS             10834..11154
                     /locus_tag="IVK82_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP6391999.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(11266..12135)
                     /locus_tag="IVK82_000923"
     CDS             complement(11266..12135)
                     /locus_tag="IVK82_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6392000.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(12231..13535)
                     /locus_tag="IVK82_000924"
     CDS             complement(12231..13535)
                     /locus_tag="IVK82_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6392001.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(13532..14041)
                     /locus_tag="IVK82_000925"
     CDS             complement(13532..14041)
                     /locus_tag="IVK82_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392002.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(14216..16351)
                     /locus_tag="IVK82_000926"
     CDS             complement(14216..16351)
                     /locus_tag="IVK82_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6392003.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(16434..18089)
                     /locus_tag="IVK82_000927"
     CDS             complement(16434..18089)
                     /locus_tag="IVK82_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6392004.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(18091..18405)
                     /locus_tag="IVK82_000928"
     CDS             complement(18091..18405)
                     /locus_tag="IVK82_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP6392005.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            18575..18835
                     /locus_tag="IVK82_000929"
     CDS             18575..18835
                     /locus_tag="IVK82_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP6392006.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            18849..19163
                     /locus_tag="IVK82_000930"
     CDS             18849..19163
                     /locus_tag="IVK82_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP6392007.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(19893..20447)
                     /locus_tag="IVK82_000931"
     CDS             complement(19893..20447)
                     /locus_tag="IVK82_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP6392008.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(20573..22502)
                     /locus_tag="IVK82_000932"
                     /pseudo
     CDS             complement(20573..22502)
                     /locus_tag="IVK82_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(22808..23524)
                     /locus_tag="IVK82_000933"
     CDS             complement(22808..23524)
                     /locus_tag="IVK82_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP6392009.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(23525..25348)
                     /gene="glpO"
                     /locus_tag="IVK82_000934"
     CDS             complement(23525..25348)
                     /gene="glpO"
                     /locus_tag="IVK82_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP6392010.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(25362..26858)
                     /gene="glpK"
                     /locus_tag="IVK82_000935"
     CDS             complement(25362..26858)
                     /gene="glpK"
                     /locus_tag="IVK82_000935"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP6392011.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(26994..28454)
                     /locus_tag="IVK82_000936"
     CDS             complement(26994..28454)
                     /locus_tag="IVK82_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6392012.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(28444..29739)
                     /locus_tag="IVK82_000937"
     CDS             complement(28444..29739)
                     /locus_tag="IVK82_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6392013.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(30071..30748)
                     /locus_tag="IVK82_000938"
     CDS             complement(30071..30748)
                     /locus_tag="IVK82_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP6392014.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(30778..31869)
                     /locus_tag="IVK82_000939"
     CDS             complement(30778..31869)
                     /locus_tag="IVK82_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP6392015.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(31970..32404)
                     /locus_tag="IVK82_000940"
     CDS             complement(31970..32404)
                     /locus_tag="IVK82_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6392016.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(32988..34445)
                     /gene="ascB"
                     /locus_tag="IVK82_000941"
     CDS             complement(32988..34445)
                     /gene="ascB"
                     /locus_tag="IVK82_000941"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP6392017.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(34442..36337)
                     /locus_tag="IVK82_000942"
     CDS             complement(34442..36337)
                     /locus_tag="IVK82_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6392018.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(36395..38170)
                     /locus_tag="IVK82_000943"
     CDS             complement(36395..38170)
                     /locus_tag="IVK82_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6392019.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(38220..39716)
                     /locus_tag="IVK82_000944"
     CDS             complement(38220..39716)
                     /locus_tag="IVK82_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6392020.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(39910..41532)
                     /locus_tag="IVK82_000945"
     CDS             complement(39910..41532)
                     /locus_tag="IVK82_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP6392021.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(41860..43173)
                     /locus_tag="IVK82_000946"
     CDS             complement(41860..43173)
                     /locus_tag="IVK82_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP6392022.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(43418..43642)
                     /locus_tag="IVK82_000947"
     CDS             complement(43418..43642)
                     /locus_tag="IVK82_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392023.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(43662..44030)
                     /locus_tag="IVK82_000948"
     CDS             complement(43662..44030)
                     /locus_tag="IVK82_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP6392024.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(44226..44936)
                     /locus_tag="IVK82_000949"
     CDS             complement(44226..44936)
                     /locus_tag="IVK82_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392025.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(44954..45451)
                     /locus_tag="IVK82_000950"
     CDS             complement(44954..45451)
                     /locus_tag="IVK82_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6392026.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(45488..45940)
                     /locus_tag="IVK82_000951"
     CDS             complement(45488..45940)
                     /locus_tag="IVK82_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6392027.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(46075..46680)
                     /locus_tag="IVK82_000952"
     CDS             complement(46075..46680)
                     /locus_tag="IVK82_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP6392028.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(47130..47750)
                     /locus_tag="IVK82_000953"
     CDS             complement(47130..47750)
                     /locus_tag="IVK82_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6392029.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(47752..48621)
                     /locus_tag="IVK82_000954"
     CDS             complement(47752..48621)
                     /locus_tag="IVK82_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP6392030.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(48644..49051)
                     /locus_tag="IVK82_000955"
     CDS             complement(48644..49051)
                     /locus_tag="IVK82_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP6392031.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(49070..50359)
                     /locus_tag="IVK82_000956"
     CDS             complement(49070..50359)
                     /locus_tag="IVK82_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6392032.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        14237 a        10670 c         8608 g        16845 t
ORIGIN      
        1 aaatatttaa attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt
       61 tttggcctga tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt
      121 tgaaataatt aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat
      181 taacgacaac cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat
      241 ttcccttttt caaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa
      301 tttttcttac cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg
      361 acaaaattta aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct
      421 tcacgtgctt gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt
      481 tcaggtgtgc ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc
      541 gcatttagtt ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc
      601 aagtcttctt cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca
      661 cgtttctcag gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag
      721 aaagtaaaga atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg
      781 tagaactcaa atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt
      841 ccgaatttcg ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct
      901 tctggtcctt ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct
      961 gctccaccga tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat
     1021 tgcaggattt ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg
     1081 atatcttttg ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca
     1141 agtaaagaga tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta
     1201 ccaaacaatt ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc
     1261 gtttgcaaca cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg
     1321 ccaatcagaa tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga
     1381 atcaaatgtc cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg
     1441 ctcttttctt caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg
     1501 cgccaagtta attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa
     1561 agcaggaacc tgctagcata tgtaattaat tatttcttca ggataggata ggaaataaaa
     1621 aaagtccctt ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt
     1681 ctagatatcc aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag
     1741 actcctgaaa tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct
     1801 gaaacattgg ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg
     1861 tctatcatag agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc
     1921 tcattttttc ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa
     1981 aagaaataac tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct
     2041 cttattcttt cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga
     2101 cgaggtatat ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg
     2161 ttattttaat tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca
     2221 ttcctgaaaa aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag
     2281 ttactcaagc aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg
     2341 aaaaagatag taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg
     2401 agaaagaaca actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca
     2461 gcttgatcgt ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt
     2521 atatggtgac tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg
     2581 ttttagttaa tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg
     2641 ggcttgcgct acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt
     2701 atgaatatat ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa
     2761 tcaaaaatgt ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc
     2821 tgatcgatgc ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct
     2881 tggccctgac aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag
     2941 acaatgaaaa aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga
     3001 aagacggctg gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac
     3061 tagaagatgt aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa
     3121 aaacaaatat tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag
     3181 cagaaaatca aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg
     3241 atgcttctac accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca
     3301 atatcgatgg cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat
     3361 tcatcaatgt ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca
     3421 tttatgaacc aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt
     3481 atttcttctt cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa
     3541 gcagttttgc gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga
     3601 tcaagaactc atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa
     3661 gatcaagcat gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt
     3721 ttttttcttt ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat
     3781 tgagataacg gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt
     3841 ccgcaatcaa cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat
     3901 aaatcagccc ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt
     3961 tctcagctac cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa
     4021 aagctgcttg taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt
     4081 gtagagcctc ccgtaatttt aaacttttat aatatatata gttcaaattt attctgaggt
     4141 ttattataac tatctattga gaaagtatca agctagcaat tccttgttta tgaattcatt
     4201 gacagaaatt tataaaaaga atattatgta ctaaaataat aaaaaaagtt ttataaaaag
     4261 gtataaaacc tttttattaa actatttaat cttgtttagt atatattttt agaaaaagga
     4321 gtggggtttt tatggcagaa atcgtcaaag tcacacattt gaaaaagagc tttggtcgtt
     4381 tcgaagcttt aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt ggatttatcg
     4441 gaccaaatgg ttctggaaaa tccacgacga ttcggacact tttagggatt ataaaaaaag
     4501 acggcggaac ggcaacgatt tttgggcaag atgtatggga agatagttta tctatccatg
     4561 aacgtctttc ttatgttcca ggcgatatct ctttgtgggg aaatttgact ggaggagcga
     4621 tcatcgatct cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa gatgaactga
     4681 ttcaacgttt ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa ggaaatcgcc
     4741 aaaaagtggg cttgattgct gctttggctg ttgattcgga tctctattta tttgatgagc
     4801 ctacttctgg tttagatcct ttgatggaaa cgattttcca agaagaagtt gaaaaattga
     4861 aagaacaggg caaatccatc ctactttctt ctcatattct cagtgaagtc gagcgactag
     4921 cggataaagt cgtgatcata cgcgaaggaa cactcgatga agaaggaaca ctcgatgaat
     4981 tgcgtcattt gactcgttcg accatcacta tagaaacaga agcagagaat agtcacttag
     5041 cacaattgaa cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa tttgctgctg
     5101 ataacgaagc tttcgatgtg attttagctg aagcgtccaa attaggtgtg aaaaaatttg
     5161 aatctattcc accgactctt gaggatttat tcatgagaca ttatgaagga tgaggaggaa
     5221 gcgaaatgac tgataaattt tcacgctggt ccaccctttt ttctcaaaac cttagccgtg
     5281 actggaaaaa gatcctgata tggctagtcg gtgtcactgc tttttctggt ggttttgtcc
     5341 ctgcgtttga agaattgact tccggacaag gattagctgg attatacgaa accttgcaaa
     5401 acccggcaat gattgcaatg gtcggaccaa ctccagcttc gaatgctgct gagtatactt
     5461 taggagcgat gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct gctgtgatct
     5521 ctgttttaca cgtggtgagc caaacgagaa aaaaagaaga acttggtctg attgaactta
     5581 ttcgttcctt tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct gaaagtattc
     5641 tgatcaattt gcttttagca atatcgatca gctgtacgat gatttttttc caagcggatt
     5701 cgatcactgc agaaggtgct tggctttttg ggctatctgt aggtagtatg gggattttgg
     5761 ggagtgccgt tgcactgttt gctgcacaac tgatgccaag ttcagccagt tcgaatggtc
     5821 tgtctttagc gtttttaggt ggactttacc ttctacgagc aataacagat gtagctgatt
     5881 cctccctttc gatgtggaat cctttaggct ggtcttattt gacctatcca tttacaacta
     5941 acaactggat tcctttgctt tatttgtttt tgttcagtct aatcttgata atcagttcat
     6001 ttgttttaga ggaacatcga gatttgaaca ccggctatat ccccgaacgt gaaggaaaac
     6061 cgtatgctaa acgttctctt ttatccttgc gtggtttatt attctatcta aataaaggga
     6121 tgacaatcag ttggcttatc acttttggtt tgatgggggc ggcatacggc tctatctacg
     6181 ggaatatgca acagttttta gaaagtaacg acttaatgaa acagatgttt atccaatctg
     6241 gcgtctcaat tgaaaaaaac tttacaagca cgatcatggt agtcttgatt tgtctagctg
     6301 ctattttacc aattgctagc atcaatcgtt tatttacaga agaaacccac ggacgtatga
     6361 atcagctgtt cgcaacaaaa atttctagga accaacttta ttggaccaat atgttactag
     6421 ctattctatt tgctatattg gcaatcctga ttagttctgg tgcactaggg ctttctgctt
     6481 tggcttcgat gaaggatgcg aaagacatgg atctattgat gtttcttaaa gccgggttca
     6541 atttcctgcc tgccgtttta gtgtttatcg gaattagtgg ctttctattc ggatggcttc
     6601 ctcgaatcgg taaacttgct tatatctact tagcttactc ctttgcactc aattactttg
     6661 gcggaatttt ggatttacca gaatggtttt ccaaaacagc tgtccctagc tgggttccac
     6721 ggatgccaaa cgattctttt gatctttccg tgttccttac gttatcatgt atcagccttg
     6781 cactgttcgt ccttggttct attggttata ggcgaagaga cttactagaa ggataataaa
     6841 aaagcatgta caatttcatg ctagcctcca aatctgggtc aaaaatccaa catttggagg
     6901 tcattttttt atttacaagt cttctaactg ctctttatca atgatccaga cttctcgatt
     6961 ctttcttttt acataaccat tctcctctaa taatttaaat tttctagata aagtttcggg
     7021 ggtcgtaccg ataaatgcag caagttcttt cattttcatt ggcaggaaaa agtgatcctt
     7081 ccctgtcgct ttagctaagt caagcagata gacagcgact cgttcttcta ctttttccat
     7141 catcaaaaaa gttgtctgct gctgtacttt tgttaatttc tcaacgttga ttgctaaaag
     7201 tttcagacta agggcgggat aatgcagtaa gagttccgag aaatcttttt gtctcaatat
     7261 gcaaatttcc gttttttcta gtgcttcgcc aaataaagac tcatttggaa cgcctagcaa
     7321 ctggctctct ccctcatatc cgcccggctc tattactcgt aaaagctgtt cctttccgct
     7381 agcagataat tgatacactt tcatactgcc ggaggccacg atcactaatt gaggttctcc
     7441 attcggcgca agaatctgtt ctcctttttc cacatagtga tgtttgacaa gtgcgtgtac
     7501 tttttcttga tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa cacataaatg
     7561 atcatgactc ataatctctc ctccttctaa acagctattt ctaataaaga aacataactt
     7621 cccttattcc aactgataat tttcttttat catagcataa acgtcaagcg aaccagatac
     7681 cagcaaatag agtggatttc tttttgtccc ttgctttatt ttccttcttt tgagacctta
     7741 agtaattgat tcctatcaat tttttaaata tttacttttt gaataatcaa aaacaaacct
     7801 tcactcgcaa gccgagagta aggtttgttt ttcgcattgc ttatttcgtg ttgtctattg
     7861 aaatttttcc gcacctagct tttgatatag tggtgctagt tcttgtaagg tttcagcggt
     7921 tatttgctca acgtgttcga tttcttgcgg atagaagaat agtttaccca tttgaaaggt
     7981 tcggtctctc atcgcttttt tcaactcttc ttgtttcttt gtatcggaca aatagtcaga
     8041 caaagctaag tggtcgtcaa gttcatgttc tggttgcggc catatgtaat aatccttgat
     8101 atcgactgtt ttgctccact ctttcagtat cgcaagcaac tggttatacg ctgcaatgct
     8161 accatatcgt tcttcgcttt tataatgttg gtacatcaaa tagatttggt attgtttttg
     8221 attcaacaaa actgctagac aggcattctc gtcttgacga gattcgcttc ggtacgcgct
     8281 ccaaaaatga ctgcgtaaat tccagccatt cgtccagctt tcgattttag gtttgctcat
     8341 gccgtacata tcaggcaaaa gtgcagctgt ctggagctga atctctttcc atttttgcca
     8401 gtgttcttta tattctttct tgatttgttc aatcttatct gttggatggc tctctttgag
     8461 ctgtttgaat gcgtagattg gtcgatcaaa caactgataa tctgattttc caagttccat
     8521 tgtttcgcct ctttccgtta tcttttgtat tctacctgaa ttttgcgata tggacaaata
     8581 gaggttgtga aaaagctgtt ctgcatcaag tcataagaac agccaaacaa aaaactgcga
     8641 tgagcatcac cgcagtttta ttgaacttaa ggttgattga tcacatctaa aagcgttgaa
     8701 aataaaatgg cactagaagc agctcctggg tcgatatgtc cgattgcacg ctctcctaga
     8761 taggaagctc gtcctttttt agctaccaag actttggtgt gttcttttgc ctggtcaatg
     8821 atttcttttg ttaaattccc tgccttcaat gcctctgtga ctggcagcca aacatctaac
     8881 atcgttttat cgtcagcttc tgcctttcct cggctttgaa tacccgctgt tccctgctcg
     8941 atcacttcgc ctaatttttc ttgagaatca atagaatcga cctcttttgt cgctttcatc
     9001 atgttcatga atgcagatcc gtataacgga ccagaagccc caccgacttt cgatatcatc
     9061 gccatagaga atactttgaa tgtatcgctg attgtttcag gatgatcggc ttgaaaagct
     9121 tcttcataag ctgaaacccc tcttcccatg ttatttccat ggtctccatc gccaatcggg
     9181 gtatctagat cactgagata ttgtttgttc tgattgattg cctctgcaaa ttgcgtgagc
     9241 cattcttgga tttcttgaac ggttagtttc atattatcac ccttttatct tgagattttt
     9301 taccatgaaa tcgtctgtac attgctttct aaagaatctt tccattgtgt ctccgtcaag
     9361 tcgatcagtg ttagagaaag accttgcata tctagtgagg tcataaaatt accgactttt
     9421 cggaaaacta cattgacgcc tctttcttcc aacagagcta aaacatcatt catgaacacg
     9481 aactgttcca tcaaaggcgt cccgcccatt ccgttgacta gtacacctgc ttcttttggt
     9541 aaattcgagt aatcatctaa aatcttagta acaagttcct ttgccagtac tttagaaggc
     9601 tgcatctttt ctcgacgata gcctggctca ccgtggatac caacaccata ctcgatctca
     9661 tcttctccta gttcgaatcc tggtttccct acttctggta cagttgctgc tcgaagcgct
     9721 agaccgatcg tcttagttgc ctgtacgacc ttttctccca gttctttcaa ctcagctagg
     9781 gaagcaccgt ttcttgctgc atcgcccacg attttgtgta caaggacagt acctgcaact
     9841 ccgcgttttc cagcagtata cgtactattt tcaacggcga tatcatcatc tacgatcaca
     9901 ctttctactt caatatcgtc catagcagct aattcctttg ccatatcgaa gttcaatgca
     9961 tcccctgtat aattcttaac gatcagcaaa actccttttc cactatcggc ttccttaata
    10021 gccgtctgaa tctgatctgg agttggagaa gtaaacacat caccaagcac cgctgcactt
    10081 aacatccctt cccctacaaa cccggcatgg gatggttcat gtccgcttcc tccaccagaa
    10141 accaaaccaa cttgttccgc tttctgattc ttggctacaa cacgggaaga ttctacacga
    10201 tgaacaagtt ctggatagct ttttactaat ccttccagca tttcttcgac aatattgctt
    10261 ggatcattca tgattttttt caatttattc ccatcctttc tttccggcat tttgtgcttc
    10321 tgttaagatt tgctgagtat catctgttat accggcagtg atggctgctg caaaagctcc
    10381 ttccactaaa ggcgcatcca ccagatgata acgttgctgc tgctcttctt ccaacatgtc
    10441 ttttgccagt tccgagttca ataccgcgct accgatatcc gcaaaaacaa aataactagc
    10501 tgcatcgctt gattcattga ctgcttctac gattttcatc gggtctgatc cgatctcgcc
    10561 atttattccg ccgagagaaa aaatttgcac ctcttcggat ttttgcatct gctcaatcat
    10621 ttctttaata ccatctgtta ctttttgact atgagaaata agcaaaatac tttttttcac
    10681 aagaatcttc ctttctttta catagttgct aacgattggt ttcgctttca ttaatgaaca
    10741 attcaagtat ataataaatt tctaattttg ggaaaatatg tgtagtgctt cttgaagttt
    10801 gttatacttt aattaaaaga gaggagtgat cgaatgatta caacaacaac gaacagtgta
    10861 gagggaaaag ccgtagtagc ttataaaggg atcgtttttg gtgaggtcat tactgggatc
    10921 catgcattta aagacttgga ggcggggttg cgaaatatct ttggcggccg ctcaaaaagc
    10981 tatgaaaacg aactaatggg tgcaagagaa gaagcacttg ccgaaatggc cgaacgtgca
    11041 aaagcattag gagctgatgc gatcattggc gtaaaaatgg actatgaagt attaggcagc
    11101 gacaatggca tgctgatggt cacatgtagt ggaacagctg tctccacgag ataagtaggg
    11161 gacatctgtt ttaataacta aagagagatg ggacatgtag tggcactcaa gaattagatt
    11221 tttcagtcta acttcgaggt actctacctt cccatcctct cttttttatt tctttttgcg
    11281 gatgattccg taagctgctg cgactaacac acccgcccct gctaaggaat aactgacaga
    11341 agctgttttt tcatttgttt ttggcaaagt ctgacttgtt gatttggatg gctctgtcgt
    11401 ttgctgaact gtttctgtcg tgcttccaga ggtcactgat ttttctcctg ccgaatgcaa
    11461 aatatctttt attcgttgga aactagctgc taagtcagct ttttttgctt caaaatcatt
    11521 agcaactgtt tgtgcttcgc tgatctttcc gtggattgct tgactggccg ctgtgatatc
    11581 cgcttgagtt tgacttagct ttccagaagc tgtttgagca acctgattta attgctgatt
    11641 gaattctttt gcttgttgga tcttcccgtc aagatctgtt tggacagctt ccgcttttgc
    11701 tttcgcttcg gcaagctttt tctcaagttg tgcacggtca tttcccactt tatttttaag
    11761 atcagctaaa tcttgatcga ttgctgcttg gtcttctttc aatttgtttt ttgcagcttc
    11821 taatgcttct tcgagttctt ttcttttatt ttccaagtct tgcaaatgat cgatttggtc
    11881 agaagaggta gaactattta ttgatgtatc ttctgaagat gaaatcgttt ctcctgaatt
    11941 atctgtcggt acattggaag tcatttctgt ttccgaggca ccgttttcag caccagaaga
    12001 gcttatggtt gttgaaggtt ctgtttgagc aggttcagaa gtttccgcaa aagctgtctg
    12061 tcccaataag aaaatactgg aaacagcaat tggccagatt aacggcttca ttcttttttt
    12121 catcactttt cccatcctta ctacttttta actaatttct tattataagc aaaaatgttt
    12181 ggaagaacca taaacacaac aggctgtttg tctaaaaaac ggtgcatctt ttattttttt
    12241 gtacgttccg ggcttttcca tgtcccgctt ccataaccta aaatcgtttt gactgctgga
    12301 atgaaagtcg tcacgatcgt tatcgcgttc actatccaga acaaaagcat atatagtggt
    12361 gcgaaaagaa ggtatttcaa ttttcttcgt cgatcatcta caatcagcga agctagcaac
    12421 tggaaaaatc ctgcgaccat ttcaaagcag atgaacacaa aactgaatgt aaaagtaata
    12481 taaatctgtt cgtagtttcc ttgatataca taatgcccga ttaatccagc aaaaagaact
    12541 acggaaatac aaaagaatat tgaccagaca atactcaatg tttgatcaat gaagatgatc
    12601 gttcttccaa tatgtttaaa aggatggagc atcacttttt tgaaattcgt cagccagact
    12661 tctgttccac cttttgccca acgttttctg tgtcgatata gcatcttcaa cgtcacaggt
    12721 acttccatga aaaagataat ctcaggagca aaaacagata accagtcatt taactgatga
    12781 tcccaagcaa tactgatgtc ttctgtcgct cgatcttgtc tgaatccgcc cacatcgatc
    12841 aaggcttctt ttcgatacat cgtatttgct ccactgtacg cataaagacc accgaaaaca
    12901 gctgactgtg ttcgtttaat gatgcccacg atacttgaga attcaaccgt ttgagatttt
    12961 gcaatcaatt tggtacgatt ttggacatcc atatttgcag tgaccgcagc aatatgtctt
    13021 gcaccagggc gaatgaaata attcacatac ctaatcagtg catctggctc cggtaccgtg
    13081 tctgcatcat tgcttaaaat caatttccct tttgcaaagg caagtccgat attaaacgcg
    13141 tgggcttttc ctttattttt ttcgatccga accacacgaa gattagcgta ttttttcatc
    13201 agtctagcta atatttctgg tgtttggtcc gttgaaccat cgtctgtcac taatacctca
    13261 tagttgtgat aattcaattt cgtcatcaaa tactcgattg tatattcgat cacaatttct
    13321 tcattatggg ctggcaccat gatcgtgatg aacggttcga cactcaaggg aacatctacc
    13381 cattcttttt gcttatgttt gtataaaaac acataacacc agacaccaat aaaccataca
    13441 aatccgccta aaatcggata agaaacgaga aaaaaataca taatagacaa acaagtatct
    13501 aagaataaat tacctgtatg ggtgattgag atcatttcag ttccacccca ttttctttat
    13561 acaaatctgc aatgaatgtc gtatctagat ttttctcttc ggagacagaa taaaaacgga
    13621 ccgtttctct ctcttctttt gttccaaaac gttccgtata cacttcttca agtaattctt
    13681 gtcttttttt caatttttca tcttcatatt caaaagattt atttactttt ttgtatagat
    13741 ttcgattgtt ccacctagtc aatacaaatg aggtaatcag gaaactgaaa aaaatcaata
    13801 caaaaaagcc gattaagaaa tacagcattt tcttttctga taaaaaagcg tacaaggaaa
    13861 aatccttggc tctttctggt ctcgttaatg ggaaaaagac ccatataaaa ggaagaacaa
    13921 cgatcatcca tcctaaaaga gccactaacg tttgccaaat ctttaaccag taattacctt
    13981 ttgaaaaata cttatctttc accaaaattg atgcaacttc ttctcttgtt acttttttca
    14041 tatcctcacc tcacgttttt ttgttacgac gtaggagttt accatacatt tcatttcttt
    14101 tcacataaag tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc tcatcagcaa
    14161 atgaaaaata ttttgttctt tttatcagtt gttcttttta ttttttatct cttaattatg
    14221 ttgctctttc caatacgaca atgcttttca ttcctctaga tatcgccgtg tacatttgtc
    14281 tgcgtcttct ttgctcatct ccatcgtttt caaatggata taagattaca tgatcaaatt
    14341 ccagtccttt tgctagactc aaaggaatga tttggcagtt ttctaatcca tatgaatttt
    14401 ttttaatcga ttcttgtagc gtttgggttt cctcttctgt ttgggtgata actgccgctt
    14461 tttctccttt tagagaaacc aaaatctcag ctagtatttt caacaattct tcagaggatc
    14521 gaaaagagcg gtattcgggt tcttttcctt gcggtcggat cgatacgatg tcgatcttct
    14581 cttgatccac agcataggtt ttgaaaagct ctgtgattgc tccgctagaa cggtaactat
    14641 ttctaagatc ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc ataatatcag
    14701 caaaagtagt ggaagaatta aagattgcct gattctcgtc acctactaaa gtaaagtgag
    14761 tttttggaaa taattcgatc aatagaccta attgtgccag ggtataatct tgtacttcat
    14821 caatcaatag atagcgaaat gctggtacat ttaccttttc aacaagtaaa tgacgaatag
    14881 ctaatataat gactgcttca tcaacagtac gtgtgggttg ccaagcataa gctgagccat
    14941 atcgtttttc ataaattctt tcaaacaact gatgctcatc cacccatgcc atttcttcga
    15001 tttgtctggt tatttttcga tatttttttt gtaaaagttt ccttgcataa gctacaatac
    15061 tttgttccga atcatcagaa atcaatttac cgaataattc caactgccta tcttctgtca
    15121 acgagagtac ctgattctgc acagcaggac tttttgcttg tttcaaaaga tgattcttcc
    15181 attcgctcat cagtgccttt ttcgttgctt gaagcctatc gatcaattta ggatgagcag
    15241 gaactttctc atatattttt tcgatcgttt ttttagaaag gactgttttt ccatttttag
    15301 taagtccacg gataaaattc ggatgatcta aaaacaaagg atctgattgt tttagattat
    15361 cgataaactt tttgctacgc aaacgttctg tctgctccga tacattttct cccgtgatac
    15421 gtttgaaata agcctcttca ccttctattt cctctgctga aagctgtgaa accagctgta
    15481 tgatggttag attcagtgga tttctttcac ctaatgatgg taggacattc gaaatatagt
    15541 cgataaaccg atgattaggt gatagaatca gcaagtcatc ggatgtcatc gtatctcgat
    15601 ataaatagag cagatacgca atacgctgca tgatagcaga ggttttacca cttcctgcta
    15661 cgccatttac caagatattt tgacttttca catcccgaat gattgtgttt tgttccgatt
    15721 ggatcgtagc agtgatagcc ttcatttcat tggtctcatc ttggcctaag acttctaata
    15781 atacgtcatc ttgaatagct acagacgtat caaaaaactt gaccaaacga tctctttcca
    15841 aaagaaattg tcgtctttct ttaataaccg tatcgatttt attttgacga accgtataag
    15901 aagatggacc aatcatgtta ttgtaaaaaa gttcggcaat tggtgaacgc caatcataaa
    15961 caagattttc atctgcatca tctgtgaaat ttgctgtacc gatataaaag gattcttttg
    16021 gctcattatc cagaaagtct acaacgactt ttccaaagta gggacttttc aataaacgtt
    16081 cgatctttgc tagctgattt tctgcgtttt tctgtttgat attcatctga tcgatttctc
    16141 tatttttcat ttcaatcatc gaaaaagtat ccaggttatc taagtaactg tcaaaattca
    16201 atcgaagctc gccagccatt tctttcaacg actggtttcc ttctattttt gccgtatcca
    16261 tcgattcccg cagttcgttt tttttatcaa ttagcttttg atacacgttc ttcagatatg
    16321 cttcttctaa ctgtttttgt gtgttgacca tacacattct cctctttttt taaagtggtg
    16381 gaaccgatta gtttacctct tttaagagtc gaatgcaagc tttttaacga gtatcatttg
    16441 ttaggatata tcagttcttc aggtcggaca gtttgatata aagaaaatgc accatcaagg
    16501 ttttttacgt ggtagccaga ctgcttcaag attctttccg ctagatagct acgtaatccg
    16561 ctatgacagc tgacgatata ttcttgttgg ctgtctaatt catttaatcg gtcccgaagt
    16621 tcattcaaag ggatatgttt ggcttccttg aatttgccct tttccaattc ttctgctgtt
    16681 cgcacatcta ataaaatttt tccttttgct agttctgtcg gcaattcatg ccattgtacg
    16741 gtttcactaa gcccttctgc aatgttcatt gctgcatatc ctagcatgtt tactggatct
    16801 ttggctgagc caaatggcgg tgcatacgtc aattctaatt ccggtaaatc gaagatcgtc
    16861 aaatgccctt tgattgctgt tgctaaaata tcgatccgtt tatccacacc ttttgcccca
    16921 acaccttgtg caccataaat ctcgccagtc tctggatgga agatcaactt cagtaaaata
    16981 tctgttgctt ctggataata gcttgcatga tcttttcctg atacatgaat gacttcaaaa
    17041 gacagttcgt tttctttggc tgttctttca cttaaaccgg tggatgcggc agtcattcca
    17101 aacgttcgta taattgctgt tccaataccg ccttgatttc ttcgagaaat tccggaaatc
    17161 gtgtccgcaa cttgtctacc ttgacgattg gctggtgaag caagagaaat aagtgcatct
    17221 tttccagtga tttgctgttt tacaacgata gcatccccca ccgcataaat atcaggaata
    17281 ttcgtttgat aacgttcgtc tactaggata cccccgcgta tgcctaattc gattccagcg
    17341 gtcactgcta gctggttatc aggacgaaca ccaatggata aaataactaa atcagcaggg
    17401 atggtacgcc catcatccag tcgcaatcgt ttcccgtggt cttcaaatcc agcgacagca
    17461 tgagaagtaa tcacttctac tccttgatga gacaattctt tttctataaa agctgccatt
    17521 tcttcatcca gtgttggaag aatatgtggt gcttgttcga tcaccatgac ttctaaacct
    17581 cttcttttta ggttttctgc catttccaat ccgatgaatc ctgcaccaac gatcacggca
    17641 cgctttggct gtttttctaa tgcatgcatc actttatcga tatctggtac atttctaata
    17701 gaaaaaacat tgtcggcttc tgttatccct ggaatcgatg gaacaactgg ttttgcacca
    17761 ggagataaaa ccaatgcatc gtactgttca atgtgttttt gaccctcatg tacgacttct
    17821 accgttttgt tttcgggaaa gatggctgtc acttcatgat ttggacgcac atctaaacaa
    17881 aaacgagcgg ataatgattc tggcgtttgg acaagaagat tttcccgttc actgatttct
    17941 cccgaaagat aataaggcaa gccgcaattt gcaaaagaaa catacggtcc tttttcaaaa
    18001 acaacgattt ctgcatcttc cattaatcgg cgaagccgtg tagcagcaga cattccacct
    18061 gctacacctc cgacaataac gattttcatc ttatttccct cctactgttc gatcaaacca
    18121 ttgattcatg ccaccacgta cattcactac atcataacca gatttcttta attctttagc
    18181 tgcttgttta ctcctcatcc ctgattgaca aatcacatag acggtctttt ctttgtctcc
    18241 atcatatctg tttatacttt gtaatggaac atttttagct ccttttatat gcccgccccg
    18301 gtattctgca ggtgttctaa catcaagtaa cgctggattt tccaaaagct tttcctttaa
    18361 ttctgggact gttatcgatt ttattgattg aaatagcaaa ttcattttct gcacctcatt
    18421 tacattttta taaatacctc atggggtata ttaaacagga gctttattaa tatgtcaacc
    18481 agaatgactc ttcatttttt tcactacttt ttatatttgc ataaaatacc gataaggggt
    18541 attatctaat tataaaaata gaggagaatg tgttatggag attgataata aaaatatcct
    18601 gaaccgtttg aaacgtacag agggacaaat tcgtggtatc caaaaaatga tcgaagacga
    18661 aaaagaatgt atggatgtca tcactcaatt aagcgctgtt cgttctagta ttgatcgcgt
    18721 aatgggcatg atcgttgcag ataatctgaa aaattgcttt gagaaacctg atagtaatcc
    18781 agaagaacaa gcggctaaac tagaacaagc gatcaagatg attattaaaa agtaaaggaa
    18841 tgatttcaat gaaagtcgtt ttccatattg atgaattaga aaaatggtca gaaacaggga
    18901 aaaacgtaaa aaacctgata aaagcttctc cagaaacgac gattgtcgta tctgtaaatg
    18961 gcatcgcgat taccggttat ctagactctg ccaatgcaga attcttagat ttgcaaggag
    19021 tcgtttttca cgcttgcgcc aatgctatgc gtgctaatca tatttcagaa agttctttac
    19081 ctgaacaagt aatcgttgta ccagcaggtg tccttgattt agttgaactg caatcccaag
    19141 gttatgctta tatcaagcct tagtcagtag aggttgcgac agaagtgatc agcttcaaga
    19201 aatttcggct tatttttcga agaagctact tctggaaaac cgtgaatcaa aggttgtgaa
    19261 aacgctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagcttct catatttttc
    19321 gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttaag gaagaggttg
    19381 tgaaatcagc ggtttgacct aagtattaga gaaaaaaacg ggaaaatagc tcctcatatt
    19441 ttgccgtttt ttgagctaat accgaaggtc agctgattga acaccgttta aggaagagat
    19501 tgtgaaaaag tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg gatttctaca
    19561 tttttgcata aatttggctt aataccgaga aactggcttt tgaataccgt ttaaggaaga
    19621 ggttgtaaaa tcagcggttt gacctgagta ttagagaaac aaacggaaaa atagctgctc
    19681 atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc gtttaccaag
    19741 aggttgtgag cctaccattt aactccgaat cacaagaaat aatttcaaaa acaacttctc
    19801 ctgtttttgg ttcggttggc ttattatcga gaagtagcta ctgggagtct atttatcaaa
    19861 aaaaggaatg cgacataggt cacacccctt ttttattttc tttgaaacat ctttcggtac
    19921 tcgacagcat acaagacaaa agattgtaaa agaccgtaag tgatgacggc aaaaatgact
    19981 ctcacccaca gcgttggtaa ttctgggaaa agcagcaaaa ggatcatgcc aacatagatg
    20041 acagcagtcg ctactttgcc ataccacttc gctccactta atttgatatt tttagatagc
    20101 ccgattaatc cagctacaaa cataaagcct tcttttatca agaatagacc aaacaacagc
    20161 caaagccacg gtcgatacca tgccatagac aaaatcagaa aaagttgtgt caatttatct
    20221 gcaaacggat caagcacctt ccctaagtct gttacctgat tatatctccg agcaatgacc
    20281 ccgtctagat aatcagacaa acctgagaca acaatgaccg ctcctgctat ataaaatgac
    20341 tgtctcagca caaaataaag ataaatcggc agcaaaaaaa tacggaatat cgataaaaga
    20401 ttcggaatcg tttgtatttc tttacgccag tctgtgctta atttcatttt atcgccctac
    20461 ctcctcagca ctcttttctt tttattttag cttacacggg gaataaaacc aatgatttca
    20521 ctgtttttat ttttctttac agacactgat attatgagct aatttgagat aatcacttgc
    20581 tgttttcatc gtttactctt ctttcttcct gtcgtttgcg catttctttt actagtactt
    20641 tatgagcttt ttttgatcct cgctctaggt cacgctgccg tttacgtgtt ttataatcga
    20701 taaacagact gtctaaatcg taacctgtcg ggtacagatc ttctgctgaa cgcaccagac
    20761 gaacacgctg acgaggcacg tggattattt ttttgtctaa atatacatca atcgtatctt
    20821 cacctatatc ttcataaact aacgcttctt tttggtattc attgacaaat atccgatcac
    20881 cttttgcaaa gagctgacgt tcttttttat tctccttcct atttgtaact gctgtcttag
    20941 gaagaaattc ttttttctgt gtccgatagg ctttttccgc caagtaacgc tctgcttgat
    21001 ggatcaattt catcgacata tccattttat gagcaatcca caaagcttgg ctttctcctg
    21061 tttcttccaa cccgcaactg atatttagga cgaagtgctt cccgatcgaa agccatagca
    21121 gccggcacaa aatcttcatg atctccggcg aatttcttga tttctccata atgggtcgtg
    21181 gcaatgatca atgccccttg ctcatacata gattccatga ttgcgatcgc tagtgcagcc
    21241 ccttcatttg gctctgttcc actgccaatc tcatctaaaa gtaccaacgt attccttttt
    21301 atctttttca aaatagcagc gatgttttgc atatgaccag aaaatgtact caacgcattt
    21361 tctaaatttt ggtggtcacc gacatcgacg aaaatctcat caaagacagc aagctctgtc
    21421 ccttcttttg ccggaacttg cataccgaac atcgccatca aagtcaataa accgactgtc
    21481 ttcaacacga ctgttttgcc tccggcattt gctccggtga taatcaagcc tcgatagtct
    21541 ttaccgattt caacatccaa tggtaccgca tgctcaggta aaaatggatg tctgccttgc
    21601 ttgataatga tatgttccga tttattgatt ctaggtgtgc ttccattgat ttctcggcta
    21661 aattttgccc gagcaaagat gatatctaac accgtgatcg tttcgatcaa taaatcgatc
    21721 acttcttcgt tttctgcgat agcaccagtc aatgccgcca ggacttgata ttcttcactg
    21781 atttcttcag cttttaacaa ctgatagtgt tcattcaatt tagaaatcac agttggttca
    21841 ataaaaaccg tcgttctttt atttgattca tcgataatcg ttccatctat tttattttta
    21901 tagctggctt tgataggaat cgtatagtat tcgccttttt gaacaatcat tgcttcttgg
    21961 atcatttctt tgtttttcgg atgacgcaag aatttcagta attttgactg gatttctttt
    22021 tcgattagtt gtaattgttt gcgtacttta cgcaaattac gtgacgcgtc gtcactgact
    22081 ttattatttt ttattttctg gtcaatcaac tcttctacat ttatcaaatc aggaagatgt
    22141 ttgctataag caaacaacaa gggtgtctgg tattgattct tatcaaaaaa ctttgtgatc
    22201 atccgactgc ttctaagaaa atccgcatat tcgatcaaat cggttggctg cagaatcaac
    22261 ccttttttga cttgttctgt cagtgcatcg atcctcggca accccataaa tggaacatgc
    22321 tgactgcttt ctaagatcaa gcgtgcttct cttgtttctt cttgacgatc caagacagtt
    22381 tgtaggtttg tcgagaccgt catatcgctg attcgttttt tgctatagtt tccaattgct
    22441 cttgcttgga tttccttttt gatccgatca aattgtaatt gactaatcat ttcgttgtcc
    22501 atattctctc cttttcttga agagatacct gtcccaaaat aaataagggc ctagagataa
    22561 ataaaaggat agaacacagc ttaaaaaagt catgtcctat ccttattaga atacaaagat
    22621 attgttttcc tgacaggtac cgtttcataa gcacaccaaa taaactttgt ttattggcta
    22681 ttcgacttac tgattagata ccagctacac tcacaaaaca taccccattc ttctaggtct
    22741 tatttatttt ccacttaact atagcaagac acattttggt tgtcaaactt tagtatgttt
    22801 tatagattta caccattaat gaatagacac ctaccgcgat aattgctccg atcattggac
    22861 ctgcgattgg tacccatgaa tatgcccaat ctgaagttcc tttattagct attggcaata
    22921 ccgcatgtgc taatcgagga ccaaagtccc gagcagggtt gattgcatat cctgttggcc
    22981 ctcctaatga aagaccgatg gctaagatca atccacctac agcgaatacg ttcgttccag
    23041 gtgcaaattc gttttgtcca aaagctagca acccaagaac taatacaaat gtaccaatta
    23101 attctgtgat cacatttgct ggataattcc gtatagcagg acctgttgca aacgttccta
    23161 aaatcgcgct ttcatctttc gttgcattcc aatgtggcaa ataagaaagc caaacaagaa
    23221 tggcccctgc aaaagctcct aaaacttgcg caatgatgaa aggaacgaca agattccaac
    23281 taattgcccc tgtcatcgcc atagctagag agactgcagg attcaaatga gctgggccca
    23341 tgaatccaga aatatagaca gccattgtta cagcgagtcc ccaaccaaat gcgatgacaa
    23401 tccagccaga agcctgtgct ttacttttgt ttaaattcac agccgcacac acaccatctc
    23461 caagcaaaac taggatcagt gtcccaataa actcccccat gatttgagtc atatcactgt
    23521 tcatttattt ctcccccttt agttcccgca gatctgattc tgcaatcaat gcttctaaac
    23581 gtttagtctg ttgcgccttc tcctcttctg tccattcaaa atatccagca attgcatcga
    23641 tcacaggttg cttgatctca tcaagctgat ctcgttcaaa caacagatga tttgtacgac
    23701 gaatgagata atcgcctggg gccaaaacca tttcttcttc taaaccataa cgtaaccgag
    23761 ctgactcagc gagactcaaa ccaggataag gtgccatttc ttttgctaat tcaaagatac
    23821 gtcgggcatt agtcccgtaa aaatcagcga tataagtagc atcttcttcc tctaaaccgg
    23881 ctgctactcc ttcttttgct aattctgtca ctgtttcttc taatttagtt ggatcgaagt
    23941 ttccacctga aatctgatat tttttagagt cgatttcttt cgtctctatt ccgtattctt
    24001 cttttaataa ttggcgtatc aatcgcaaag ctccttctgc catcttacgg taatcggtga
    24061 ttttcccacc agataaagtc agcaaaccat ctggttctct ttctaaagaa ctacctctgg
    24121 aaatcgtaga aggtgctttt tcatcacggc tgttttccaa atggttcaac acgtcttcta
    24181 cttcagcacg agaaactttg ttttccttat attcacttac tgtatcgaca actttattga
    24241 agctcttgtc tgaaatcgat ccattatctc caccattgta atcagaacca gaattaccaa
    24301 tcaacagtgg acgaagtcct gcccagcttg cttcgatatc attcaatgtg atatttgctt
    24361 ctggatagcg atgattgatc acatccaata gataatctac atcttcttgt gtgactttcg
    24421 gatccgtaaa atctccttgg taatccgtat ctgtcgtacc aaagtaagtc ttgttttctc
    24481 ttggaatagc aaaaaccatc cgcccatctt gctttcctgt atcgaagtat gtgggttgcg
    24541 gtacaggcag ttttttcgca tctacaacta aatggatccc tttagttgga cgcattttag
    24601 gagagactgg acgcgtaaaa ttcaagtttc gtactttatc tacccaagga ccactcgtat
    24661 tgatcactaa ttttgctttg atttcgatca cttcatctgt cagcaggtca cgagctttga
    24721 cgccaacgat ttgatcgcca tcatataaaa agccaaccgc tttcatttta cttactagat
    24781 aagccccatc ttcggcagcc tttttgatat tatcaatcac taaacgggca tcattgttgc
    24841 ggaaatctag atagacaccc gcgcctttta gcccttcttt tttcaaaaac ggttctcttt
    24901 ccaatacttc ttctggtgtc agcgtataat tctcatattt ggttcctgtc acatttgcca
    24961 gtttgtcata aaggtccatt gctactttga ccgagaacat attgaaggtt gttgctcctt
    25021 cgccctcata gattggcaaa agcattggat ctggttttgg gatgtgtggg gcaatacttt
    25081 gtacgactgc acgttcacca actgtgtcag ctactacttc cacatcaaat gtcttcagat
    25141 aacgaatacc gccatgaaca agtttggtcg agcgagagga agtcccttct gcaaaatcct
    25201 gcatttcgat caatcctgtt ttgattcctg atgctgctga ctggactgcc acaccggcac
    25261 cagtgattcc gccaccgatg atcaacagat ccaattcttc ttgctgcatt ttttgaatgc
    25321 tatcttgtct tgtcttgttt gaaaacatat ggatttcctc cttattcaaa tgcttgggta
    25381 gcttttactg cttttttcca accgttgtaa agtttttctc ggcgctcttc ctccatttga
    25441 acttcaaata gcttgccagc ttcataaaat tcgcggatct cgtctgtatc tttccaataa
    25501 ccgacagcta atcctgctaa aaatgctgcc cctagcgccg ttgtttccaa gttttctgct
    25561 cgttggatcg gtacattcaa gatatctgtt tggaattgca tcagatattc attgtttgct
    25621 gcaccgccat ctactttcaa tacaggaatc ttgattccag tgtcatcttg catcgtatcg
    25681 atgatatctc tcacttgata agcaatcgac tgtaaagtag ctttaatgat atcttctttt
    25741 gtcgtaccac gtgtcaagcc aaacatagcc cctcgtgctg cctgatccca ataaggagca
    25801 cctagcccaa cgaatgcagg aacaacataa acctcatctt cgcttgtaga gcgtttcgct
    25861 gcgtcttctg aatcgctagc tttttgcaac atttgaagtc catctctcag ccattgaatc
    25921 gaagaaccag ccacaaaaat acttccttct aaagcatagt aaacctttcc atttatgccg
    25981 taaccaattg tcgtcaataa attatttttg gataattgcg gtttttcacc ggtattcatg
    26041 acaataaatg aacctgtacc ataagtattc ttgaccatgc ctggttcgaa tgccatttgt
    26101 ccgaataatg cggcttgctg gtctcctgcc atccctgaaa ttggtgcttc gcttccataa
    26161 aaatgataac cttgcgtata accataaact tctgagttag aagtgacttt tggtaacatc
    26221 actcgaggaa tatttagtaa atctaaaatc tcctgatccc aatctagctg atggatattg
    26281 aacaacatcg tccgactggc attcgagtaa tcagtaacat gtgcttgtcc atcggtcagt
    26341 ttccatacta accaagaatc gatcgttcca aacatcaatt cgcctttttc agctcgttct
    26401 tgcgcgcctt ctacatgatc aagaatccag cgtatcttcg ttgctgagaa ataggcatcg
    26461 atcactaacc ctgttttttc gtggaaaaat tcttgatagc cttcattttt taagttatct
    26521 gcaattccag ctgactgtct ggattgccaa acaactgcat gatagattgg acgtcctgta
    26581 tctttttccc atattactgt tgtttcacgc tggttcgtta tgccaatccc agcaatctga
    26641 tttggtttga tccctgattc gatgaacgct ccggcaataa ctgactgtac agaattccag
    26701 atttcatttg catcatgttc cacccaactt tcttgcggga aatattgcgt gaactctttt
    26761 tgagaactgc cgatgtgtct tcctttcttg tcaaagataa ttgctcgtga gctagttgta
    26821 ccttggtcaa tcgccatgat atacgttgat tctgtcattg tgattccctc cattatatgc
    26881 aaacgctttc tttaactact cttatcatac tactgtttta aaaaaagaaa ctgtcaaaat
    26941 tttattcttt ttttcttttt ttgacagttt tttattatga agctattttt taatcaattc
    27001 ttttcttttt tcagcgtctc tattcttctt ttcaatcgaa taatgtcgta aggtgacgca
    27061 aattctgaca gataatattc acactgtttt ttgtttctct ggcaaaatgt gatcaccaaa
    27121 tcatattctt taccttcttc ataacttcca atctcaatac cgccgattcc cctcagttct
    27181 ctgatcagat attgttgaaa agcaatacga taaatcggca gactttctaa atcgattcca
    27241 atcactactg gttttgcaat ataaaaatcg atcatcaaaa gaacattggc atactggatc
    27301 atcaggtaac taagctcact atcttcagct cttttcctcg gcaattgctg caccgctgtt
    27361 tcctgtaatt tcgccagtaa ctgtgttaag gaatgcccga gcattttctg ctgctgttcc
    27421 aaaagccgat cacgctcata gacttcgatc tttccaacaa agaaaaacca ttcattgttg
    27481 atctgggaaa tttggtagcc tactgccttt tcttctttga ttttcaaacg acgaggacga
    27541 taatgccata acatcgtttc ccgaatgtaa gtatctaaaa cagcggttgg taactttttg
    27601 ctccgtgtca aatcatactg ataaaatatt tcttcgtcta ctatcgaaaa agaaacaaaa
    27661 aagctgtaaa acatcattgt ttcgtataca ttcagttctg ctgctgttcg actaagatgc
    27721 atcacgatga ttggatcgat tgcttgataa agccggtccg actgatagag taatttcttt
    27781 tctttcaaag ttgcgtacgt acttttttca ttcaatagtc tttttttcgt aatcccgagc
    27841 cagcaagcaa tctgtgcttc tgctgatgcc gtaaacgttg tttctaatac acggtccaat
    27901 cctagaatga acggtctttt ttctggtgta ttttgcaaaa aaagcggtcg ctgatcttcc
    27961 gggatatatt ggaatagttc ataataaaaa tatcgtatct gtagctcttc tccgtataat
    28021 tgaccgtttt tgatttgtaa tccaaattcc tctaagagct ggttcaattc tttgatcttt
    28081 cgaaaaacag aagactcact gatattaaac gctgtcgcta atcgaacgat ggatacttgc
    28141 tgatgttcta ataaatagac gagcatctta aacttcaagc tttcctgcaa taaaaacaat
    28201 agaatttttt ccaaacttac atcttcgtcc aacacaagca gcgcctgata ctcatttcgc
    28261 tgtacttcta cttttttttc catcattcgg ccaatcaatt caatatcttc caaatattga
    28321 tcaaacgagg cttttgacag ttggacatgc tctctcaaac cattgataga ggctgttccc
    28381 ccttgcagaa caaggtttcg caaaatcagg atctgtaact gctcttttct ttctaataat
    28441 gcttcaatct tcataggcag tattcgccac ctttgaccac accatatctg gcacacggta
    28501 ttctggctgg aaaaatagct catgttcttc taactgttca aacgtccatc cttcttctac
    28561 tgcaattgtc agttggtcaa gaaggtggaa catctctttt ttgctttgga gctgtgctcc
    28621 aagaatcttt ttttcagaat caaatagaaa tttgagttta actttgcttt gatttattgc
    28681 tgtaagcttg tagtctacgt atcctgtttc aattttttgg ggataaaaaa tgctctcttc
    28741 ttctgtcaaa cctacgcttc ctaagaaata gccaaaccac tgattgccga ctggacgata
    28801 ggttccctga tcctttgtct gtgagccact aattgtcttg ctgacatttc gtgctgtacg
    28861 gacagcgtta tttactagag aaacatacat tttttcattt gttggacgaa aagttacttg
    28921 gatcgcatcg ccaatcgcat aaacgtcttt ttgagacgtt tgcaagtagt catctaccca
    28981 gatcgttcca tcatcattca atgttaattg ttcttcccac atctgattgt cgggacgaga
    29041 atgattagcc agcaaaacaa gatcatagtt ctcctttctt ttttccgtct ctacaatagc
    29101 ggtttcattt tcggtcaaag acacaacttg ttcttctaaa aaaagtgaaa gcccttgatt
    29161 tttcatttcc tttactaacg gttctgtcat ttcaggatct agataacgaa aaagaattga
    29221 ctttcggctt tcgtaaagat ggatttgttt ttttcttgat gccaaccctt cagctatttc
    29281 gattcctgct tgtcctgcac caataattgc aattttcttt gcattcatga tcttttgctg
    29341 aagagccgtt acgttttgta tctttttaac tgggtgaata agcgttgatt ctgcagaaat
    29401 atttcgaaac ttttgatttg aaccagtcgc tagaatcaaa cgatcaaaat ccaatttttt
    29461 tcctcctgct aaaacgactt ttttttcttt caatcctatt actgtctttt ctgtgtaaac
    29521 atggattcga tagcggtcag tcagttcttc tttcgtgatc caagaaagtt gtttttcact
    29581 ggtaaccttt ccttttaata tcaaattgaa cccgctggga atccatccta acttcgattg
    29641 cttttcaatc aaatatattt cacttgttgg catcaattgg cgaagataaa tagccgcatg
    29701 aatccctgca aacgacccgc cgacaattac aatgttcatc tgcattcctc tttcttataa
    29761 ggtgttctac tgctaacttc tcaatcagaa aacacagtat tcataactcg ctttcaacct
    29821 tatgatacat agcgatgcag cagaatcaac cctctccatc ataccgtgtt ctaggtctgc
    29881 ttctcaataa tgtactagtg gacctaattg aagagtatca tttctgaaga gatgtttcaa
    29941 aaaatgtact cttataaaag aaaaaaactc gccttttacg tacctctgtc caaataggaa
    30001 acagaaaaga acgaaaagac gagttttagc gtgtgtaatg taatagttag aatagacaca
    30061 tcatttattt ttaattcttt aatgcttcaa caactgcttc gcctaattga tgagcagatt
    30121 gaggattttg ccctgtaaga agccgcccat caatttgaac cacttctgaa aaagctgctc
    30181 cagactgata gtttgctcct ttttctttca aagcatcttc tgctaagaat ggtacttcat
    30241 tcgttgtacc atttgcttcc tcttcttcat tactgaaccc agtcactgta cgtccagcta
    30301 aaatagattt tccagactca tctgtaactg gcaacaatcc tacaacaccg tgacaaacag
    30361 ctgagaccag cccttcattt cgatagattt gtgaagcgac tttcccaaga tctattgctt
    30421 ctggaaaatc ccacattgtt ccgtggccac cagcataata aaccgctgca taatcatttg
    30481 catccacatc atcaggacgt aaggtatttc ccaaggcccg atttctgaaa tcttcatctt
    30541 cataataggc ccaatcgatt tcatccatat tcaaaacact ttgcggttct aaaggtacat
    30601 atccgccttt aggactgaca aaatctgcat cgatcccatt ttttactaat acatcataaa
    30661 aatgagtcag ttcacttaac cacaaacctg taggacgctc gatcttttcg tacttttcta
    30721 catttgttaa tacgatcaat actttcattt tcccatcctc tttctataaa aattgcgtta
    30781 ttgataacgt gctttcaaga aattatataa tgggcttggc aaataaagct ctttatatgc
    30841 tttttcaaaa ggtttcgtta caaattcatc ctcacggttt tccaataatt tctctgccca
    30901 gtcaggatcg acgatcattt gctgtccaat tgctgcaagt tctgcatatt tcaatacctg
    30961 ctctacatct tcgcgattgc ggatcccacc gacaccaact aaagggatct ttccttgaat
    31021 cgtgttatgg accacttcaa gaatcgattt atcgttgtat ttatcagaac gagctattcg
    31081 atcatacata tttaatgaaa catgcagata atctagtttt gactcacgta atcgttcaag
    31141 aagccagatc gtgtcttcaa aacggatacc tggtgtttca tattcttctg gtgagaaacg
    31201 ataccctacg atgaatggtt ttttagcata ttgttcaacc gctgagaaaa ccgttgttaa
    31261 taattcttca ataaaacgat aacgattttc caagctaccg ccccattgat cctctcgtct
    31321 attcgaatgc ggagagaaaa attgttggat caaatacgta tttgctccat gcaactcgat
    31381 tccatcaaat cctgattcaa tcgctctacg tgtggctttg ccaaatgctt ggattgtttc
    31441 aacgatttct tcttcactca ttgctcgtgg tgtttccgca tcaggacgtt ttgctgcaac
    31501 tgcacttgca gatacaggct gttctccaga taaagttgct cgacttgtca ttcgtccacc
    31561 gtggaaaatc tgaataatcg cttttgcgcc ttctccttgg attgctttcg ctagctcact
    31621 taatcgagga atcatttcat catgagcgat gctgagttca cctggccagc cttttccgat
    31681 atcttggaca tttgcagcac cagtaataac agcaccaaga cctttagaac gttgtttata
    31741 ataagtgatc tcatcttcgg tgatcactcc attgaaaaag ctgagttgtg tcgtcatagg
    31801 cgacagcatc aatcggttct tcaattgaac tcctgaaggt aaagtaattg attgatcaaa
    31861 ttgtgacatt gtcattcctc ccataattcg ctctgattaa cctatcgaga atcatctatc
    31921 attaaaccac tcgattagtt gtacatgcaa ctaatttgct taaaaaattt tatttttttt
    31981 gtaacttatc tgttgcatca ttcattgtct gggctaattc tttacttgct tcatttccca
    32041 aaactgcttt gtatgactga tgaagttctt cccagctacc atcgacttcc ttagcaaatt
    32101 ggcgtccttg ttctgtaaga taaactaatg acaagcgacc ttcgctaact gttttgacgt
    32161 atcctttata ttctaatttt tcaacaaaac gagcaatcgt tgatggggcg gaatagagca
    32221 tttgtgcaat atctttttgt ggtattccat ctttttcttt taatatcaat aaaataaaac
    32281 cataagttgg tgccagacct gtccttctaa acgcttcatc agctaattta ttcaacatgc
    32341 gatctagctt tttcacgcta aagaaaagac attcatctaa cgctttttca attggctttt
    32401 ccatttcatc ccctccattg gcgtacatat catagtatga tttttatttt ttgtcaaaaa
    32461 acacggaata atgtccgcct cctctgtcta gatttgaaaa aaagaagtag ccatcttcaa
    32521 agaatttcgg cttgttttcg acaaaactcc ttctggaaca ccgtgaatca gaggttgtga
    32581 aaaagtcgtt tagttttgag tatgaagaaa aattttgtga aaatggcttt ccacattttt
    32641 gcataaattt ggcttaatac cgaaaaactg gcttttgaac accgtttata ttagaggttg
    32701 tgaaaaagcc gtcctgcatc aagtaataag aacagccaaa caaaaatagt tcctcatatt
    32761 ttgtcgtttt ttgagctaat gccgatggtc agctgattgt acaccgttta ttgagaggtc
    32821 gtgagcctgc cgttgaactc cgagtcacaa ggagcaattt caaaaaacag cttctcatgt
    32881 tttatgacta tggtgacttg tgatcgagga gttggcagga gaatactgtt tattcgtaaa
    32941 gaaggggctg tgacaagact tttgtcacag tcccttattt ccttttatta taggatttcg
    33001 ccatttgatt ggatgacttg tctgtaccaa tcaaatgatt ttttctttaa ccgtttcttt
    33061 gtgccattcc cttcgtcatc ttgatcaaca tagatatatc cataacgttt tgacatttgt
    33121 gaagtagaag cgctgacgat atcaatgcag ccccacgacg tataaccaaa taaatctact
    33181 ccatctttga tagcttcctt catctgctca atatgctgct tcaaatagtc aatgcggtaa
    33241 tcgtcttgaa cggttccatc ttcttctact ttgtctatag ctccgagtcc gttttcagct
    33301 atgaacagtg gtttttgata cctatcatat aattgattca aagcaattct caaaccctta
    33361 ggatcaatct gccagcccca ttcacttgat ggaagatacg gatttttaat tcctccagtt
    33421 attaagtttc cttggacccc ttcccaattt tctggatgag cactgactgc actagacatg
    33481 tagtagctaa aagaaacaaa atcaacaggg taggctgcta agatttcttc atcacctact
    33541 tctattttta agttgacacc tttttcttca aacattcgtt gcgtatacgc tggataatat
    33601 cctcttgctt gaacatcact aaagaacaaa gaagttcttt gctgctcaat tgttttttga
    33661 atatcatccg gatcacaaga atacggaaca agaagctgat agctgatcat acaaccgact
    33721 tgtgaacctt tgataatttc atgacaagct tttgtagcta aagcacttgc tataaactga
    33781 tggtgtgcac cttggtattt cgcttgttcc aaatggggat ggttttcttc aattactcct
    33841 acgctaacaa aagggtgatg ttttacacag ttgatctcat tgaaagtcat ccaatatttt
    33901 actttatttt tatagcgttt gaatactgtt tcagcgaaac gaacgtaaaa ctgaataagc
    33961 tttcgattag gccatccacc atattctgta accaaatata acggcatttc ataatgggaa
    34021 agagaaacga ctggctcaat cccatatttt agacattcgt caaacacttg atcataaaaa
    34081 aataatcccg cttcattggg tgtttcttca tcaccatgag gaaaaattct tgtccatgcg
    34141 atagacatcc gatagcattt aaaacccatt tcagcaaata aacggatgtc ttctttaaag
    34201 tgatgataga aatcatttcc gtgtctttta ggataataat acttgctgtt gggatcctgc
    34261 gccgctttta cttgctgatg cgtcatcata tgccattgat ctgtccattt ttctttaggt
    34321 aaatcgctgt cgaatgtata cacatcagac acagataatc ctttttcatc tacatcccat
    34381 gcaccttcac attgattggc cgctgtcgct ccgccccata aaaatccttc tggaaatttt
    34441 ttcatatcga tccccctaaa cttgctagtt ttcttgtttg attatttttc ttatgactag
    34501 taatgatatt tttgaatcta ctactatttg taacaataac cattgtggtc ggatcatatc
    34561 cctctttgat taattcttgc aaatctactt ccatcaataa atcgccttta cgtacttttt
    34621 ttccatcaga tactgcactt tgaaagtatt tgccatttaa attcactgta tcaataccga
    34681 tatggatcag tatctctgct ccttcttcac tgactacccc aattgcatgt ttcgttggaa
    34741 aaactgttga aacttccccg tttacagaac tgtagatttt tccgtccgtt ggaatgatcg
    34801 caaacccttc tcctaaagat ccactagcaa atgttggatc gtttacttca gataaatcaa
    34861 ttatttcacc tgctgcagga gtacttaaca ctatatcctt tgaggattct tcttcgattt
    34921 catccttgta gccgaaaaga aaagttagca tacaggaact gatcatcgct aagaaataac
    34981 tagcaactag ccaaacaaat ccttcaccaa tataaatcgg gaaagacaaa atacttcttg
    35041 tagcaactgc caaagcaccg gctcctgcgg caccagctac tccaccaaat atagctccac
    35101 atatacaagc aatgtaaaat ggtcgtttat atttaagagt cacaccataa acagcaggtt
    35161 cagtgattcc aaataatgct gtgataaatg cagataaaga taaacttttt actttcttat
    35221 ttttcgtttt caaaaatacc ccaagtactg ctcctgcttg cgcagctacg gcagtcatac
    35281 atgctggacc tagcgtatca cgaccgtaga aagaaagatt gttggtcatt actggtacaa
    35341 gtccccaatg taacccaaaa atcactaaca cttgccaaaa tccgccgata acagcaccag
    35401 ataaaacagg gctaaagtta taaacagcta aatatccagc tgcaatcagc tgacttgcat
    35461 aatctgcaat tggacctaca actaataaaa taattgggac catcacgatg atacaaatga
    35521 atggtgtcaa taaatttcga atactttcgt gcaagctttt gcgaatctta ggctcaatat
    35581 aagaaagaac ccatacagct aaaattgctg gaataaccgt acttgtatac cttgctagaa
    35641 tcacgggcat ccctagaaat cgcaaggtta tgctatcact atatgcactg acaatcgtag
    35701 gataaacgag agctgctgca ataaccatag cgatgaaacg atctgtattg aacttttttg
    35761 cagcagtata tgccaaaaat ataggcaaga aatagaaaaa tgcatcagct gctgcattca
    35821 aaatgatata agttcctgat tcagtagtta ataaattcaa actcgtacat aaggcgagta
    35881 ctcctttgat caaaccactt cctgccaaga cgcctaaagt cggggcaaaa acagcggcga
    35941 tcatgtctat aaaagagttt aaaatgcctt ttttctcagt tcctctcttt ccactagatg
    36001 catcatcttc tagtttcatt tgtttggata atacgtcata cacttcattt actgcatttc
    36061 ctactacgac ttgaaacatt cctccagctt cgactacagt gatcacacct tctagttgct
    36121 ctaccttctc cttatctgcc ttcttattat cttttagggt aaagcgtaac cttgttgcac
    36181 aatggtaaac attgctgaca ttttcttcgc ctccgacaaa tcggacaata tctttcgcta
    36241 attgtgaata gtccattttt acttccccct tgttaattaa tgccgtagca atccttctta
    36301 caagataagt atagttctta gaaaacggtt tctccattca attagtaccc cttctaatag
    36361 cgaacgaaac tgtacaatag tgtgtatggt ttacttagcc gagcaaatca ctcactgctt
    36421 gaaagaaaac aagtatatcg tttctcttat agtcaaagga gacaaaaaaa acacgcttga
    36481 tcttcctttg ctgatgtata agtggtatct caagctgata ctctgtgatt tttgtttcta
    36541 cttggctttt gataagacag cacaacaaag tctcggcatt taacggaagc ataactaggt
    36601 cagaactagg agagattagt tgctgaatgg aatctatctt tttatggatt tttatttgat
    36661 tttcttcttt gtagtaatga tcaagcagtt ttgttttctt tttttcttca actatggtca
    36721 gcttttcacg caaaatctga taaatctccc aagattcaaa ctcatctaga acactaggta
    36781 gataaatcaa atcatcccac tcaaaaataa gctcttgctc agtagtcaaa attgcatcat
    36841 attccgttat tttgtttgat tgatttttga aaagatacac tggttctatc cggcattctt
    36901 tgatccacat gttcatactt tgttgaatct gtttttttag tgtattcaaa atactaattg
    36961 gctggttact aacaatcagt atctcccctt gttttcgaaa aggattcaac gcttcggcaa
    37021 gatacaaaac aaaataagcg ctttcagcta aattgggacg taattgaaaa tgtattggga
    37081 agaaacgccg aattaaataa atttctaaag gataacggga gatgatcttt tgtacaaaat
    37141 gattcatggt attatgtccg gcatccatcc tcgttttcat ttgtttgata tgggttagca
    37201 aaaattctgt ttcatggaac aataacgaaa caggaagatc taaaaaacga ataatcgttt
    37261 gctcaaatgc tcttagattc ttctctattt ctttgttttt ttcatcatta atcatcaaat
    37321 aatttaactc taatagtcga ttttcaattg cttgtttttc ttgattggaa aaagaatagg
    37381 gtagttctga tgataacttt tttgtgaggg tctctaccat cggaataaat aatgtatacc
    37441 tgtttgtttt ttctaaaagt tcttctcttc gaagaatggt caaaacgaga taccgagaaa
    37501 atcgtatgaa gtcctctcct gaaacaatat aattatatga tataagaacc tcttttaatg
    37561 tcttttggac agtcggcatg atattggaaa aaattgtact gatttgttga ggaaaccttg
    37621 cgtgttggat caccgtttct gttccaacaa gagataagaa aattcttttt gaattttctt
    37681 cagcaatgat ttttagtcct tttgtcgacg aaacttccag ttctaattta ggattgcgct
    37741 cgatccaacg caaaatcgtt tttatttgta atgatacggc tgtttttgaa agaaagaaaa
    37801 tgtctgctat cttttgcata ctgacatacc ctttttgaaa caataagtac gtcacaatag
    37861 ttgaagctcg gaaatccatg ctggaataga ttttatgttt cgtcacctct tgtatgattt
    37921 gctgtttagt ttcagattca attgattcta aaaaataacc tgaacgttta tttgactgga
    37981 taatgatttt cttctcgtag atagcaagca tatttttgat ttcagtttgg atcgttttct
    38041 tgttggtacc taaagcttca gctaattctt cgcttgttat ccaatgattc ttatttctta
    38101 atactacaag aatttggagt tgtcgtttat ttaagtccat ttgcttacac ctcctctttg
    38161 ttataaacat acaggaaaaa cctctcaaat gacaatagtc acttgcagag gtttaaaaat
    38221 catttgaaga aagatctata ttcaaggttt ttaccattag actggataat ttgtttataa
    38281 tattcaaaac tatctttttt caatctccta aagcttccgc tcccatcatt ttgtctatcc
    38341 acataaataa agccatatcg cttcctcatt tcccctgttc ctgccgatac gatatcgatt
    38401 ggtccccacc ataagtaacc aatgatttgg caaccatctt ttattgcctc agcaatttcc
    38461 tgaagatgta tttttagata ctcacaacgt tttggatcgt ggattcgatt caattcatct
    38521 ggtgcttcat ctagtccaat gccattttca actatgaata atggtaattc ataacggtct
    38581 gtcaattcat tcatgacata acgaaaccct agaggatcaa ttggccaact ccaaggttcg
    38641 ggagattttt ccttcaaaaa gggattatcg aaaccatttg ctccgccagt atctacgcgc
    38701 atttctgctt cttttttata gacagcgcta cgataataac taaatgcaat ataatctgcc
    38761 gtgttagctt gcaagcactg ttcgtctcca tctaacatta ctggtgcaca gtcttcttcc
    38821 ttccatgtcc tgtaaatata agctggataa tgccctcggc acatgacatc ggagaaaaac
    38881 catgaattgc gtttaaattg cagtgaacca aatacattct ctggatcaca gtcatatgca
    38941 taagtcgcta atgcagaaaa gctacacata atacccatct tagcatttgg atcgattttc
    39001 tttaataatt gagtagcacg ggcgttcgct agaaacatat gatgacaggc ttgataaatc
    39061 actttttttg ttagtgtagc aatgggttca agtttatctc tagcagggaa tgtaaccaat
    39121 ccagcagctg cataaggcat acgaaaagca ttattgatct cattaaaggt catccaatat
    39181 ttcactttcc cttggtagcg ttcaaagacg gtctgcgcat aaaattcaaa aaagtcgatt
    39241 aattttcgat tttgccaccc cccatactct cctaccaatg ctaagggggt ttcataatga
    39301 ctaagagtaa ttactggctc gatatcatat cgatttaatg tattgattag tctgtcataa
    39361 aatacgagac cagcctcatt tggcttctgt tcgtctcctc ttggaaatat tcgtgcccat
    39421 gagatgcttg ttcgaaaagc ttttaaacct aactccttca gtaaacgaat atcctcttca
    39481 aatcgatgat aaaaatcaat cgcttcatga gaaagataat ggagtgattc gtctaaagca
    39541 atctcccaaa tctttttttc ttcattccac tgaatactag gatgctggtt catgatcccg
    39601 ataagcgtat ctgtcacatt aggtaatttg ccgtcttcca accatgcacc ttcacattga
    39661 ttagcagcta ctgctccgcc ccataaaaaa ttttctggaa atatttcctg atccataact
    39721 atctctcctc gctatcaaca atttcttact tgaagtatag ctcgattcat caaaacaaga
    39781 tattctaact atttcggcag aagagaagaa aaaaattgaa tgctactctt catttttaat
    39841 atttatatat aacaaataca taagacgatc cccgaaagaa aacactcgtt aaccatctaa
    39901 gcaaacgatt tattttactt ccaacacaaa tgcttcataa ggtcgtaaat caactttgcc
    39961 gtctggtaac tgtttcagag gataatttga cacaataacc cttaaagctt cacgtggaat
    40021 agtgaacgtc tgcttttcac tggacatatt acaaataacg agccattgct ggtcttcata
    40081 tatgcgagaa taggcaaaga catgctcagg cgtcttttcc actaattcat agtctcccca
    40141 gacgatgatg tcatgttttc tacgcaaatc gatcaatttc ttgtacatat aaaaaacaga
    40201 atcttgatct gctaacgctt cttctgcatt gatttcttta taatttggat tgactcgaag
    40261 ccatggaatt ccagtagtaa acccagcatg ttcacgggcg ttccattgca taggcgtcct
    40321 agcattgtct cttcctttcg ccttgattga ttccaagatt tcctcttttg taaatccaga
    40381 agaaatcctt tcgttataca tattgattgt ctcaatatct tttgcttccg cgatatcatc
    40441 gattgggcaa ttcgtcatgc ctaactcttc tccttgatag atataagggg tgcctttcat
    40501 catatggagc aagatagcaa acatcttggc actttctttt cgatacacat cgtcatttcc
    40561 ccaacgagaa acaattcgcg gaagatcgtg attattccaa aataaactat tccatccttc
    40621 atcacctaaa gatgtttgcc atttggataa tacttttttc agttcagaga tactcaaggg
    40681 tttcaaatcc catttgtctt tgccttcttg ctgatctaat cctatgtgtt cgaattggaa
    40741 gaccatggat aattcatttc ttttaggatt ggaataaagt ttagcgattt cggtagttgc
    40801 tccccatgtt tcgccaacag tcatcagatt tttgtcacca aaagttgctt gattcatttc
    40861 ctgaagatac tcatgcagtt taggtccatt acttgtgatt tctttatccg gttctttgcc
    40921 aatcatatcg atcacgtcca tacgaaagcc cccaatacct ttatcgatcc agaaattcat
    40981 catttgataa atctcttggc gaacctgttt atttttccaa ttcaaatcag gttgacgctt
    41041 gctatacaag tgcagaaaat attgccccgt tttttcatcc aactgccaag cagaaccact
    41101 gaatattgag cgcaatctat taggctcttt tccattgacc ggatcacgcc aaatataata
    41161 gtcccgataa ggattttctt tatttttacg ggcctcaatg aaccacggat gttcatcaga
    41221 agtatgattc accaccagat ccatgatgat acggatattt cttttctttg cttcttcaat
    41281 cagttcttcc atttcttcca tcgtaccgaa ttctgccatg attgcttgat aattactgat
    41341 atcataccca ttgtcatcat tcggagactc gtatactgga gaaagccaaa ttgcatcaat
    41401 acctaaatac gccagataat ccaacctgga aataatcccc cgcaaatctc caataccatc
    41461 ttgatttgaa tcttgaaaac ttcttggata gatttgataa acaactgaat ggtgccacca
    41521 tttttgttcc atgattttcc tcctataaat ggatgtttct attttttgct taatcattta
    41581 ttttagtctc tctcactaaa aattgtaacg ctttcaaata gctattgcac cttgttatcg
    41641 gtaacagaat aaaacaaaaa aacagctgag gaaattccct caactgtttt ttgaaaggat
    41701 tgatttatgc agggaagaat gctgatttca caacttcttt ataaacggtg ttcactcagc
    41761 tgtcccttgg tattagctca aaaaacggca aaatataaga aactattttt tcgttttttt
    41821 ctctaatact caggccagaa cgctgatttc acaacctctt tatttcacat tcaatatcca
    41881 catgaatccc acaaatacaa agaacagacc atacatgatt ggagatactt cttttcctct
    41941 tttagctgct aacatggtaa ttggatagaa gataaaacct aatgcaatcc catctgaaat
    42001 actataagtc aaaggcatgc cgatcaagat caagaatgaa gggatcgcaa tctctaagtt
    42061 tccccattcg atttgtcgca aagattgtgc catcaatact cctaccataa tcaatgctgg
    42121 tgcagttact tgtgaagtaa caactgatag taatggagaa aagaacaagc ctaacaagaa
    42181 gaaaatccct gtagtaatag ctgtgatacc tgaacgtcct ccaacggcga taccagctga
    42241 tgattctaca aatgcaccaa caggagaagt accaaataag gaacccgcaa gcatagctgt
    42301 tgaatcagaa gctaaagctt tccctacacg aggcatcttg ttgtctttca tgaaacctgc
    42361 ttggtttgct aatccaacta aagttcctgc tgtatcaaag aaagtcacaa gcaagaatgt
    42421 cagcactacg acccacattt gaagagaatt gatgtcccct acatgtttta atgcaactaa
    42481 aaatgttggt ttcaaacttg gtgctgcggc aacaatttca gaaggcattg gaatcaaacc
    42541 tgtgaccaga cctaaaatcg ttgtagctgc catcccgatg aagatgccac ctggtacacg
    42601 ccgaacaagc aagatagcag ttacgactaa tccaaaaatc gtcaaccaag tagaaccaac
    42661 gttgagtgat cctaatccta ctaatgtaga ttcattcgaa acgatgatcc caccttcgct
    42721 cagaccaagg aaagcgataa atagaccgat accgccagaa atcgcatatt tcaaatctgc
    42781 tgggatcgca tcaatgatca tttcccgcag cttgaagatt gtgatcaaaa taaaaatcaa
    42841 agaagcaacg aatacgccgg ctaatgctgt ttcccatgga atccccattc ctaaacaaac
    42901 agaataagca aaaaaagcat tgatcccaag agcaggtgcc gttccaattg gataacgggc
    42961 taataccccc attaaaacac agccgatcgc actggctaat gcagtcgcag taaacactgc
    43021 tccttcatcc attccagaag cacctaatac agttggattt acaaataaga tataagccat
    43081 tgagataaat gttgtaaatc ctgccagtac ctcacgtttt accgtggtgt tcaatttttc
    43141 tatttcgaag taggaaagta ttttttcctt catcttccat ccccctaaat ccgaacatta
    43201 taaataaagt ccgttaaaat ttcgatttat taataaaaca acgtgtttta tcatatacta
    43261 aaacaaaaca taagtaaaca aaaaattcgg attttaagca aaatccgaac gttccatctg
    43321 attaaatccc tttatctcaa agttcatgaa cattcacttt ggattacaag aatttcttta
    43381 ttgtgttaat aaagtttctt cattctagtc tcaaaaatca atagtgtgat ttgattttac
    43441 catttcgttt ctgttcttct ttgatgaatt cttttgtatc gacaggattc agattattta
    43501 gtccgccaaa acctgtatcg accatttttc cccagccttt tgtcttcttt tttcctgatt
    43561 tgagattctt tgctcgaagc ttctcactat gttttgctcg ccgatacttc aaataacctt
    43621 ctaatcttcc cattagatcc actgtttttc ctccttttta ttcaatcaga cgactcagct
    43681 tttcctataa caaaataatt gccttcaggg tcagaaaaat tgaaagtcaa gatgccattg
    43741 ttatctgtga tttctcctgg ttcttccatg ttctcgtaga tagtttcaaa ttcatccgta
    43801 aaaaatacta aagaaggcgg tggtcctaat acctctggtg aaaacttctg cacaaatact
    43861 tttgggaata cgcctaattc taattctttt gagatgctga gtacaatact cttaaattct
    43921 tctggcaatt cgattgtttc agaaatttct gcaccaattt tttctacaaa gaatcggcaa
    43981 atcatctcta cgtcattgac ataaagcatc acacgtgttt tttgtatcat catttgaccc
    44041 ttctttctat ttgattttct ttcctgtcta taaactgatt ccattatatc acgaaagcgc
    44101 aaacaccttg tgtttgatac tattcaagaa aaataaagca atagtttcta ggattgcttt
    44161 cctccacaaa ctttttttac aaaaaaaaaa cggcaatgaa tgccgctctt cttatttcga
    44221 ttcttttata attctgcttc tgattcatct ttggttagtg tgtgtctaac actagttacg
    44281 ccaaatgttt gatcgatttt ttccttcaac gcttcgattg cctgtgcgcc acgtgccgct
    44341 tgttcagcat tgatggattc aatgaccagc accgctgctt gtgtttcttc cgtcagcatc
    44401 gttctttggg cttcacgcca gttgagacag cgacagatcg ctccttgttc atcatagtag
    44461 atgatttctt ccggaagtgc cggagcatcg gaatctgcac ctaaaggcag gaatgattcg
    44521 ccaccttggg cttttcctaa atgcagattt cctgcgatct tttcgatatc ttctccgccg
    44581 caaggtacgc caaaagacaa ggaaatacta ttatagatat ctacaagcgg attgattggg
    44641 aaaaattccc gttcttgatt tgcacgtttc agtaaagctt caatagaaga acgtgcacct
    44701 tttttagtct tgaatgtggt aaatgcctga cgccactgag caatgacttc gttttggctg
    44761 aatgtttctt ctgtaaggta atttcttgct tcttttgctg cctcattcaa taattgatga
    44821 tacgtttctt cactttcttt tgttacgtgg ttatcgatac catttacgga taagatttcg
    44881 atggttgctt caggaaatac ttcccagaaa ttggtatcca ctattacttt tttcatctgt
    44941 tcacacctct tgattagaat aattgcttga tgaatagata gtctgtttgg ggatcatctc
    45001 ccatataaaa aacatgttcg ctgtatttga taaatccttt tttacgataa aaagcgagcg
    45061 caacatcgtt gtgttcccaa acaccgagcc aaatggctgt tttttgcttt tcttttgctt
    45121 tttcaatagc tgtatctagt aaaaatgtac cgataccttg atgtttaaat ttttttcgta
    45181 catagattcg ctcgatttct aaaaaatgat cagccatttc ctcagattga ctcgttcctg
    45241 tatttagttt caggtaaccc gcaggctctt gatcgatact cactaggaaa aaatcggttt
    45301 ctttatcttt tagctcttct gtaagttttt ctagtgcata agcttgtgtc aagtaatcag
    45361 ctaaatgctc cggtgaatta tcttttccaa atgtatctgt aaatgtttcg atactaattt
    45421 gttgtagtaa gtctacatct gaaggcgtca tttttcgaat ttcgtattta ttcacggctt
    45481 cttcctttca atagatcctt ttattgcctt ttttgacaaa attccaatct tcgctaacat
    45541 tcttgcatac tttatttaaa agatattcca attgttttgc ttcttcgtca gaaagaccat
    45601 ttaatgcgac tgcattggag tagttgtttt ctcgaatgat gaaaggatag atttctgctc
    45661 cttttttagt cggatagagt ttcttgattt ttttattttc tttatcttcc aaacgttcga
    45721 tcatcccatt ggattctaat ttcttgattg ctcgggcagt cgttgtacga tctactttga
    45781 tcatttctgc tagtttttct tggatgattc caggattttc gcaaatacgt actaaataaa
    45841 gatactgtcc tcttgttaaa tcgacttctt tgaattcgat attgctaata gaatctagcg
    45901 ctcgggcaat cattccgatg tctcgaagga tttcagccat ttcatcccac ctttattttg
    45961 tttgtaagac tagttcacct ctttttgttg caaatgcaac tattttcaat aaaaaaatca
    46021 agttcttttt taataactag taaagtatat tcttttcttc ttttacgaaa aagctcaatc
    46081 acaatagatc tggtttttgt tttctaaaat aagtaactgt ttcttcgttg ttcgcttttc
    46141 tgatagcttt ggcaactctt ctatactaaa aaatcccatg ttggaagttt cattattctc
    46201 aataaacttg gcctcttcac cgtggatcgc acaagcaaaa acaagtttat aatactgaaa
    46261 aagctgtgga atatcttttt gtttattcgt atcaaaaact gctcgcaact gagtagtttc
    46321 tactgttaat cctgtttctt catagacttc tttacgcaca ttttcttcag gagacaatcc
    46381 tatttcagca aatccaccag gaagtgacca ttctttcgtc cgtttatctt cgattagtag
    46441 gaccttgcca tcttttttga tataggcacg aacatctact tttggtgttg gatgtccttc
    46501 tccaatcgtt gtgagcgctt ttatttcttc gaaagtttct tttcctattt cactgatcag
    46561 ttccaatgaa atcgtccgga tctcctcaaa acgctcttta tcaaatacat cttttccata
    46621 atatagacca gcttgagaaa gtgcgagtag tcttttgtat tgagccaact tcttgttcat
    46681 tctcttccct tcttttttag caaatgtatg actatgtaat caagtataga agactggttt
    46741 aattacggca aataaatata atgaaatctg caataaataa aagaatgtta aaaaactcga
    46801 gaacaaaaga cattcgactt ctctggggct gtgaataaat ggcgttcaaa agcttgcatc
    46861 tgcggtaata agccccacca aacgaaaaat atgacgagct attcttgtct ggctgttctt
    46921 attaattgag gcaggacagc tttttcacaa cctccccact aaacggtgtt ctcctaccaa
    46981 ctcctcggtc acaagtcaca atattcatca aacatgagaa gctgtttttt tgaaattgct
    47041 ccttgtgact cggagctgaa cgacaggctc acgacctctc ggtaaacagc gttcaaaagc
    47101 caatttttcg atattaagcc atttctttgt tacttattgt cattgatttc tttttctaat
    47161 ttatccaata gtggagtgta atccacttga gtatagtctg ttacatcaaa ttcatatagt
    47221 gttcctaact ggaatttgtc atattttcgg tcggcgatga tctttttgaa tgcttctttc
    47281 gtaatatgat tggtcccttt cgttcgatct tccaatacgt caccatagtg ataatgatcc
    47341 atgatatagc tcaaatgacg atctgattca cgatcacgtt tgtaccgacg ttgccataac
    47401 acttcaaagt ctgctgttag gcgaatcgtg atcacctgat atccgtatct atctgctaat
    47461 tcttggagtc gtgctttttg cttatcacta aaaggatact cggacacaat cactttcttt
    47521 cctgcttcca tatacaaatc aagtgctttg taatagaaag gataaacttt cgtcctttct
    47581 tgttccgccc gttcttccaa attgttaaac ccgatggatt ctgcatagta ttccttgatt
    47641 tcatcgagcg tgatggcata catatctgga aatcgttccc gtagtttatt cataagatat
    47701 gtttttcctg taccaggact accagccaga agaatgaaat actttttcat ctcatttatt
    47761 tcctaagtta attggacttt ccaagttcat aaacagtcca atcttatgct tgacaatttc
    47821 ttttactgct tcatttgctg gaatcacatt atggcgaaga gacttgttga tttctgtttc
    47881 actaaaggtt tctttagcta ctttcgtcca gttgactaaa agctccgttc caacattgaa
    47941 tttgcggatc ccgttattga tcaattcagg ataatcttct tctttcacac ctgttcctcc
    48001 atggataact agggggacat caactactgc atcgatttct ttcagcaagg gaatgtttac
    48061 ttctgttttt gatttgaatt gtccatgatt cgtaccgata gcaatagcta atgcatccac
    48121 gcctgtagct tctacaaatt tcactgcgtc ttccggtctt gtatatacct tgtcattttc
    48181 agcgacatgg attccttctt ctgtaccgcc gattgttcca agttctcctt ctacggaaac
    48241 accgtgcgca tgggcatatt ctactactgc ttttgtcttc aggatatttt ctttgaaagg
    48301 cagatgggac ccatcataca tgacagaagt atatccgctg tcgattgctt ctttgatatc
    48361 atcaaagtct cgggcgtggt ctaagtgcaa gactgcatca accatttctt cttctgcaat
    48421 agctgtcatt acattgacta acacatgcat gccgatatat ttagcggtat cgacacttgt
    48481 ttgaataatg atcggcgaac ccatttcttt tgctgcgcct agcatagcag gaagcatttc
    48541 taggttatgc gtattaaaag ctccaactgt aaaattcaac tcttctgctg tttttgttac
    48601 ttctttcaat gttgtataca tcttttattg ccaccttatc tttttatttg tttcctttga
    48661 tcgttacttg ttttgcaatt tgtcgcatct catccatttt gcccatgtag taatcgatcc
    48721 cttgatcatc accagcacga gcagcatctg cacgcttctg gttatagagg ctcatcagtt
    48781 ttttataccc atctccgatc gtctgtttat tttccaaact cttttctaaa tattggatgg
    48841 cttgatcgtt cacatacaat tcagaaaatt taagtccaac ttgttcataa agtttcgcta
    48901 atttatgctt atcttgggta gtggaaatct cttgtttcaa ttgtgtaatc tctgattgaa
    48961 tcttttcggt cgcttctggc gatagggctt tttcttcttt tgccagaact tttttcggtt
    49021 ctttttcttt tttcttaaaa aatccaaaca tcattttccc tactttcaat taaaatgctt
    49081 tcaaaactgg gcttaagatc caggcatata agagtgctgc ggctactgta tctacatcgg
    49141 ttgctgtagc attgttgaat cccatcgaat taaagatcgt tactagcaaa gcgggcaata
    49201 atgtaataaa gaaaccatga gcaataccgc caatgatcgc tcctcggcgt ccgccaactg
    49261 cgttgccaaa tatccctgct gtccctccgg caaagaaatt ggttagcata ccaggtaaaa
    49321 tcatcgctaa gccgaacatg ggcaaagtaa acatggcaat aatcgtacca atcgttgtcg
    49381 taatgaaacc taaaatgaca gcatttgggc tataaggaaa gaaaactgga caatctaagg
    49441 cagggatcgc atcaggaact aatttcatag ctatccctcg aaatgccggc acgatttcac
    49501 ctaacagtag acgtacacca gctagcaata catacacacc cactacgaat tggatggctt
    49561 gtaaaaaggc aaacatgata tagttttgat ctcctgataa ttcagaagcg aatggttcgc
    49621 ctgcaaataa aacagtgatg atatatagcg ggaccatcac gaccatgaca gacaaataag
    49681 tatcttgcag aaattcgaaa tgtgtcggca acttgatatc ttcgatcgac tttttatttt
    49741 cgcctttctc accaaaaagc ttagcaacac cggcttcaaa taagtacccg atcgtacaaa
    49801 aatgacctaa agcaatatca tttgatccag tgattttacg aatgatcggt tgcgctactg
    49861 ccggcatagc aatggcgaaa caagctccga taaaaccacc gaccaagatc aatacgatcc
    49921 ctcgcagacc ggcaaaatag ccaaagactg tcgtcatcgt tgccatccat aaaattgctt
    49981 gaccagttaa aaaaatgtac ttccattttg tgaaacgagc caaaataata ttgaatacaa
    50041 aaatggctaa gaacgtcagt gcaatatctc tacctaaacc caaatcattc attgcttgac
    50101 cattgataga ttcaatagaa gggatgatcc cttgcatatg aaatccttca gtaaagattt
    50161 tcccgaaata agttaaactt ccaacgatga tgctcgaacc ggcactcaac acttgaaatc
    50221 ctaggagtgt ctttaacgtg ccagagatga cttgtcctgc tgatttcttc tgcagtagta
    50281 gacccaacat agcaatcaat gcaatggtga tcgatgcctg agtcaaaata ttttcgatga
    50341 taaaattgat aacgcccatg
//
LOCUS       DACCCS010000016        43678 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.016, whole genome shotgun sequence.
ACCESSION   DACCCS010000016 DACCCS010000000
VERSION     DACCCS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.016"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(105..629)
                     /locus_tag="IVK82_000957"
     CDS             complement(105..629)
                     /locus_tag="IVK82_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6392033.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IVK82_000958"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IVK82_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP6392034.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IVK82_000959"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IVK82_000959"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP6392035.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IVK82_000960"
     CDS             complement(2351..3031)
                     /locus_tag="IVK82_000960"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP6392036.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IVK82_000961"
     CDS             3157..4026
                     /locus_tag="IVK82_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6392037.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IVK82_000962"
     CDS             4052..4567
                     /locus_tag="IVK82_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392038.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="IVK82_000963"
     CDS             complement(4614..5177)
                     /locus_tag="IVK82_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6392039.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="IVK82_000964"
     CDS             complement(5232..5411)
                     /locus_tag="IVK82_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392040.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="IVK82_000965"
     CDS             complement(5488..5997)
                     /locus_tag="IVK82_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392041.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="IVK82_000966"
     CDS             complement(6093..6473)
                     /locus_tag="IVK82_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP6392042.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6802..7551
                     /locus_tag="IVK82_000967"
     CDS             6802..7551
                     /locus_tag="IVK82_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP6392043.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7735..9012)
                     /locus_tag="IVK82_000968"
     CDS             complement(7735..9012)
                     /locus_tag="IVK82_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392044.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9091..9735)
                     /gene="pcp"
                     /locus_tag="IVK82_000969"
     CDS             complement(9091..9735)
                     /gene="pcp"
                     /locus_tag="IVK82_000969"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP6392045.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9934..10878)
                     /locus_tag="IVK82_000970"
     CDS             complement(9934..10878)
                     /locus_tag="IVK82_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP6392046.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10880..11566)
                     /locus_tag="IVK82_000971"
     CDS             complement(10880..11566)
                     /locus_tag="IVK82_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP6392047.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12040..12360
                     /locus_tag="IVK82_000972"
     CDS             12040..12360
                     /locus_tag="IVK82_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP6392048.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12514..13419
                     /locus_tag="IVK82_000973"
     CDS             12514..13419
                     /locus_tag="IVK82_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6392049.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13416..14189
                     /locus_tag="IVK82_000974"
     CDS             13416..14189
                     /locus_tag="IVK82_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6392050.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14370..15794)
                     /locus_tag="IVK82_000975"
     CDS             complement(14370..15794)
                     /locus_tag="IVK82_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6392051.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16005..16451
                     /gene="nrdI"
                     /locus_tag="IVK82_000976"
     CDS             16005..16451
                     /gene="nrdI"
                     /locus_tag="IVK82_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP6392052.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16516..18498)
                     /locus_tag="IVK82_000977"
     CDS             complement(16516..18498)
                     /locus_tag="IVK82_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6392053.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18512..19408)
                     /gene="murQ"
                     /locus_tag="IVK82_000978"
     CDS             complement(18512..19408)
                     /gene="murQ"
                     /locus_tag="IVK82_000978"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP6392054.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19411..20472)
                     /locus_tag="IVK82_000979"
     CDS             complement(19411..20472)
                     /locus_tag="IVK82_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP6392055.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20683..21210)
                     /locus_tag="IVK82_000980"
     CDS             complement(20683..21210)
                     /locus_tag="IVK82_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6392056.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21277..22779)
                     /locus_tag="IVK82_000981"
     CDS             complement(21277..22779)
                     /locus_tag="IVK82_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP6392057.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23037..24158)
                     /locus_tag="IVK82_000982"
     CDS             complement(23037..24158)
                     /locus_tag="IVK82_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6392058.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24284..25225
                     /locus_tag="IVK82_000983"
     CDS             24284..25225
                     /locus_tag="IVK82_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP6392059.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25375..26271)
                     /locus_tag="IVK82_000984"
     CDS             complement(25375..26271)
                     /locus_tag="IVK82_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP6392060.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26540..27880
                     /locus_tag="IVK82_000985"
     CDS             26540..27880
                     /locus_tag="IVK82_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6392061.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28098..28541
                     /locus_tag="IVK82_000986"
     CDS             28098..28541
                     /locus_tag="IVK82_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP6392062.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29045..30463)
                     /gene="nhaC"
                     /locus_tag="IVK82_000987"
     CDS             complement(29045..30463)
                     /gene="nhaC"
                     /locus_tag="IVK82_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP6392063.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30866..32089)
                     /locus_tag="IVK82_000988"
     CDS             complement(30866..32089)
                     /locus_tag="IVK82_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP6392064.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32120..32959)
                     /locus_tag="IVK82_000989"
     CDS             complement(32120..32959)
                     /locus_tag="IVK82_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP6392065.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32976..33656)
                     /locus_tag="IVK82_000990"
     CDS             complement(32976..33656)
                     /locus_tag="IVK82_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6392066.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33658..34731)
                     /locus_tag="IVK82_000991"
     CDS             complement(33658..34731)
                     /locus_tag="IVK82_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6392067.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(35378..36049)
                     /locus_tag="IVK82_000992"
     CDS             complement(35378..36049)
                     /locus_tag="IVK82_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6392068.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36136..38814)
                     /gene="mgtA"
                     /locus_tag="IVK82_000993"
     CDS             complement(36136..38814)
                     /gene="mgtA"
                     /locus_tag="IVK82_000993"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP6392069.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39473..39910
                     /gene="ndk"
                     /locus_tag="IVK82_000994"
     CDS             39473..39910
                     /gene="ndk"
                     /locus_tag="IVK82_000994"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP6392070.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39932..40798)
                     /locus_tag="IVK82_000995"
     CDS             complement(39932..40798)
                     /locus_tag="IVK82_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6392071.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40958..43051)
                     /locus_tag="IVK82_000996"
     CDS             complement(40958..43051)
                     /locus_tag="IVK82_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP6392072.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLQFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        12612 a         9149 c         7717 g        14200 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aacagaaagt
     6601 aggtcctcca agacactctt cctgttccca atataataaa tgaaagtaag aaattatatt
     6661 ttcaagaaaa caaacagaaa attatcataa cacaaattat ttataattga aagactagtt
     6721 ttttttcaag aaaagcatac aatagattgt gttagtaaat acagaacaca ctacatttag
     6781 tgtcttaagg agtcctcata catgaagtca tattttcagt ttttagctca tcagctaaaa
     6841 ggctggccgc aacaaaatta ttatcttttc tttttcagtc ttggctgcca ggtcatgact
     6901 cttgtcaata gtcccatcac ttggctgtcc gttattacat ttatcggtac cacgcttggt
     6961 gtattgtgta ttttatccat caatgcggca aaatcagtca acggtatttt aggtattttg
     7021 tctgcgattt gttttattat cgttgggttt tcagcaaaga actatttaag tatcggtgaa
     7081 cagattgctt atatgatcac attggatatc cccgtattgt taagtaaaga atggaatcat
     7141 aatatggctt caaaaatccg aaaattcact ggaaaaagct ggtttatcgc aatcatcagt
     7201 actttccttg tctatcttgt ttcaggattc ctgattggtc gttatacaga tgatcctcgg
     7261 ccgtgggtag atgccattgc tttttctatt agcttgactg ctggtatcat cagtttcttg
     7321 cgttacaaca accaatatta ctggtggtta gcttcagggc ttgctcaaat ggtcctttgg
     7381 tatatttcat ttacgcatgg atctgcaagt ttggcgatgt tcgtgaacag ctctgtttac
     7441 ttgctgaatg atgtccttgc atttaccgtt tcaccttggt ataaccaaaa agaacgtgaa
     7501 cgtatgcaaa aaatggaaga agaatactac aacgcacaac aacacgcata aaaaaactga
     7561 gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga
     7621 atcagaagca actccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc
     7681 gtgaatttct tcttatttct tggagttaaa cgcttctgtc ccagcctcta ttttttatag
     7741 taattccttc agatactttc ttgcctcatc attgaaacgc agtgattctt ctaacttctg
     7801 ataatattcc aacgcttgtt tctgagcttc agtaaaacga taatgttttc ctttccattt
     7861 gtcgattgtc tcttgcacat ttccagattg gatatctttc actactagtt gccatttttc
     7921 ttttgagact gtacggtctt tcttatcagc agattgtata gcttctcgtt ctttacgcaa
     7981 tacttttaaa aaaccatttc tgactatccg aggaatgctg tcgaagaacc cttctgtaaa
     8041 accatagcga caatattgct tccaaataaa tagatagatt tttggttgga tgaatcgttg
     8101 gtttttcgca aaatattcca tctgagcagt gacttctggt gttcgttgac gttttgtctt
     8161 tctcaacacg gaaataaatg tttcaaaata agcattagga tgtactggca cttcttcata
     8221 tacgatggat tgccaatctt tacgtaaaaa tgcgcgatag ttgatcaaga attcttctac
     8281 atcctttact aattcttctg caaagtcgtc aaatagttct ttttttatct gtcgcttttt
     8341 tgactgccca ttgatgtgtt cacttatttt cttgatttca ttttccaatg cttgataatc
     8401 cgctatgtaa atcggcattt ctacttggcg aaattctgcg agccgctgag ctaattttat
     8461 atccggtaaa tatggctgtt tcatccgacg attttggtac aagacaaaac agtatttttg
     8521 ccatagtcct tcaggaaaat cgatattctt ctgcttaagc cataataatt cgtctaaggc
     8581 cagatcaact tttgtcattc cctgatggat cgaagctgcc actgtcggag agacatgcag
     8641 gtgatcggca aagtggttga tgcctagttt taggatcttt ccggtttttg gcgatttaac
     8701 gatgtattgc acttttaatt ctctaccaca ctcacaatac aatttttgct ggcttcgttt
     8761 tgttcgaaaa aacggattga tcttgaagtc agaaaacacc caatctgttg cagctaaata
     8821 atgttcttcc ataaaacgag agctgattag atatcggcgg tgcttatcga ctagttcccg
     8881 ctgttttttc gttaattttg catagatctc ccaacgttga ctgcttgtgt attgtttaga
     8941 aattttgatg ggatagcttt tgctgatttc ttttggaaac cattcttcaa agacggattg
     9001 ccattcttcc acggctgttt ctttcctttc taattacaaa aagctggcta ctaacataac
     9061 gttagcaacc agccagtgtt tcttgctaaa ttagtgagtt gttccggcat ttccggaaat
     9121 atcttcttta ttttctactg ctgcttcaac tgcataagtc aaagaagaag caatgtcttc
     9181 taaagacata gaaggcgtac ccactggacg atcgatcact tgatcgggtg agaatggcac
     9241 gtggatgaac ccacctcgaa ttgccgggaa ttgtgtttcg atcaaatgaa gcaaacgata
     9301 catgatatca ttgcagacaa atgttcctgc tgtataagag atgtacgctg gtagaccatg
     9361 atcttgtaca ttttttgtca ttgctttgat tggcaatgag gtaaagtaag cggctggacc
     9421 gttttcttct aaagccgtat cgaatggctg atttccttca ttatcaggga ttcttgcttc
     9481 tgctagattg attgcgacac gttcgaacga gactgctgac cgcccacctg cttgtccgac
     9541 acagatcacg acatctggtt ggtgctgttg gatggcttct ttgactactt gactgctttt
     9601 ttcgaatacg gtcggtactt ccactttgat gatttcagca ccgctgattt catctggcat
     9661 tttttttact gcttcatagg ctggattcac tttgtcccca ccaaatggat caaaacctgt
     9721 tactagtact ttcatctgtt gttcctccgt ccgattgatt taacatatgg ttatctttta
     9781 tcataccatt ttccttgaac agcgtaaata aaggaaacaa tagtttaaat aacataacag
     9841 atcaaactta atatattctt ataaaaaaat agtgttccac tacaatgtta gaagaggttg
     9901 cgacaattat tctgtcacaa cccctctctt ctattattta aatagaatca tataagcaat
     9961 ttggaaaact agcataaata acgcaataaa caactggttt ttgatcactc cgtatttgtc
    10021 tttcatctcc agcatcgcta caggcacgat attgaagtta gcagccatcg gtgtcagcag
    10081 ggtcccacaa aaaccgcaag tcaatgcaac cattcctatt aacgctggat tcgctccatg
    10141 actaaatacg aatggtgcac cgatcccaac cgtcatcacg gtgatggccg caaatgcatt
    10201 ccccatgatt accgtaaata tcaccatgcc aagagcgtaa atgatgatgc cgatgttcac
    10261 atttccttct ggaacgatcg tacctactgc attggaaatc actgtcccaa ctccagcgct
    10321 tgtaaatacc gcacctagag aagctaaaag cattggcaac atgcttaacg gccctactgt
    10381 gcccagcata tctgctgcat catctaaaaa tgtacttggt cgattatctc gagaaaagaa
    10441 catcaagatt agcatggctg caaaaacacc gacgcccacg ccgactaaag cacctaaatt
    10501 ggtaaataga gcaaacaaga tcgcaaagac accgatactc aaagctggaa taaaaatttt
    10561 cattcctagt ttgtctgaca ttttttccat atattctttt gatggaagcc ggctttctcc
    10621 tttgctgact ttcttagcaa ttgctggtat ggtcatcgca aagatcaagc caccgttcac
    10681 gattgccgga agccatcttc ctcctgcaat aactacccct aagaccgtcc agaaaaaggc
    10741 tgacccataa cgatgaggat tggttttatc tgttaaattt cttactccag tgtaaatcaa
    10801 tacgagtccc atcacgatat aaaggatctc cagtaattta tcgcctaata aaatatcagg
    10861 atttgtaaaa aagtccatct tattgcccct ttcctggata atatttcttc gctaatttct
    10921 tttctttaag atagaagtag acacttgcca caactaaagc cgtgatagcc actggaactt
    10981 ccactaaagc aagctgtccc aatgttacgt catatcctag atctttcagt gttgattgga
    11041 ctagcaacgc ccctgctcct ccgatgaata gtacttggcc aaagaaccag cagacatttt
    11101 ccatagctga agccatacct tttagttctt cttcatgctt aggatggact tctaaccctt
    11161 ttgattcgat ggtccccaat gccatcggta aaatgattgg acgaacaaat ccagcaactc
    11221 caccaaaact cacgttaaaa gccgcaaaga tcattcgaaa gatcccataa atatctacaa
    11281 tcataccagc agatactttc ttgaatcgct tgatcaagga agccgctgat tctttcagac
    11341 catttctttc aagcgttcct gttgccaaca taatgatgat aaaaatcgcc atcccgcgat
    11401 tgtttacaaa acttgtacct aaaagttcta acattccttg aatgccaaga ccgccagtca
    11461 aagctgtaac aactaaagca accattacga tcaatatcga gtctaattta aatgcaaacc
    11521 cgagaataac aatcaagacg cctaaaaggc taagataatt ttccatatat tttcctccat
    11581 aaaatgacaa tattttttta attataaagc aaaacacata aaaaatatag ttgttttctc
    11641 acgaaataga cactattttt aaatattttt cgttattttt ttatcacaaa aattatctta
    11701 cttattgtaa ggtaaagctt tttttaattt attgcccgat aatatataga aataaggtct
    11761 agttactgtt ttttttaaat gattaataaa taacaacttc tttctatgaa attgcaaagc
    11821 attcatcttc ttttccgcta acgatatatc cgtcagtgct tggtacactt tactaaaaag
    11881 tatgagattg ccgatatctg gataggtgtg ctatactaca gaaaaagata tacaatctgc
    11941 caccgggtag gataaagagg atgatcattc cattaggtct ttgaagggat gattttcctc
    12001 tttttgtgta tttttttata gttaaggagg aatgtaagta tggcatggat ggaattaatc
    12061 atagcaggaa ttttggaagt attctggtca acgatgatga aatggagtga tggtttttct
    12121 aaaatcaatt attcttttta cacagtgatt ggcatgattg ctagttttta ctttttgtca
    12181 aaggcaatca aaagtttgcc gatgagtctc tcttatccta tctggacagg gattggcgca
    12241 gttggttcgg tcataattgg tgtggtcttg tttcatgata agctgaatct ttccacttgg
    12301 ttctttgtgg ggatgctact catcagtatc attggcatca agattactag cggccactag
    12361 acacttggaa taagtactga tcagaaaaaa taagcctttt tatttaatat tcaatttttt
    12421 tatgaaaaaa taagaaataa gtccaatgac ctagcgactt ctccactcat caggtgtatg
    12481 cttgtactat ctatagaaaa ggagtgaata tttttgaaaa gtctttcggt ccaagaatta
    12541 tccaagaact ttggtcacaa acaagccctc agcgatgtca gttttagctt atcccctggc
    12601 aaaatcgttg gattagtagg tcctaatggc gctggaaaaa caacgatcat gaaagctatt
    12661 ttaggactga tccattactc agatggaaca atctgtattg gtgaaaatga gatttctccc
    12721 tctagtcata aagggttgga acaagtagga gcattgatcg aatacccggg tatctatcca
    12781 ttcttaacag gctatgatca tctgaagtta ttctctgaaa ccaacgatgt atctgctatc
    12841 gacactatcg tgaatcagct caagatggaa aagtacattc acaaaaaagc gaaatcctac
    12901 tcgttgggaa tgaaacaaaa attgggaatt gctcttgcct tactgaacca tccgtctatc
    12961 gttattttag atgagccgat gaacggtttg gatccacaag caacaagaga tgtgcgaaaa
    13021 atcattaccg acttggctac tcagggcacc tctttcttga tctccagtca cttactcagc
    13081 gagcttgaaa aaatggtcga tgagctgatc ctgatcaatc aaggaaagat catcaaacaa
    13141 tgtacaatgg ctgaattgaa ccaaacagcg caagatttca tcgttatctc tacaacaaat
    13201 gatctagctg ctcgaacgat cttgacagaa gctggatata ctttggaaga tcctgataaa
    13261 atcaagcttt taaaagaaaa cgaagaatta ttaggaaccg tgatccagct attgtctgca
    13321 caaaatatcc atgtgacaga cgtccagcat ctgacgaatg atttggaatc ttctctattg
    13381 gaaattctgg attcagtaaa gggggaagaa gaaaaatgac attgcttgta aaacaagaat
    13441 tatttaaatt gatcaagaaa aagtcaactg ctgttttatc tgttctatta gttgttttgc
    13501 tcataggaac agcacttcta gccaaaaaat acacgacgat cattgatcca gttgaaatga
    13561 ctgctcagct ttttagtgcc acatcttgga ttgtctttat catgattgca gctgcaagca
    13621 cgatcatttc gatggaagca caatatggta cattgaaaaa tctcctttat cgaaagtatt
    13681 ctcgaggaga aatattagta agcaaatgga tcacattagt tatttattct gtttatttat
    13741 accttttagc tattattgtc actgtgttga tgaaactgat tcttttccct tcgatttcct
    13801 tcacggagaa ggtttctact ggtcaaacac tgatccaaag cttgttcact tatactttgg
    13861 gtagttatat cggtttatgg ctgattttaa gtttagtgtt gatgattgct tgctttatca
    13921 atagttccgg cgcatccatc tcagcaggaa tcgtctttta ttttgcttcg tcaatcatat
    13981 ctggtatcct tattgcactc atccaaaaat gggagtggat caaatggaat ccgatcagta
    14041 tgttgaatct gcaaaaccaa attggaaacg aagagatcat gaagcaattg actcatcttt
    14101 ctactaatca aatgctattc ggtaatttag cttacatcgt tttgttctta gcattaggat
    14161 atctcgtatt taagcgaaaa aatgtatgat gactcatcaa aaaagaggct gtgaaagaga
    14221 cgttcagcct tattgccgat tggctgtctc ttaaacaccg tttattctga aaaaggagct
    14281 gtgacaacat ttgtgtcaca gctcctttcc tagtatgttt ttgaataaac gacaggctca
    14341 caacctctct ttatacttct tttatacttc tatacgtgaa gcatcccagt ggcaaataaa
    14401 accattgcaa tcactccgac catcgtcaca cctataagta aaatcttatc taagtggata
    14461 tgttgtacag gctcgttttg ctccttacgt gctttgaggt aaaaataaat gcctggcaag
    14521 aaaagaacaa gaacaagcat tacttcatta aaggctacga ctagcatagc ccatacttga
    14581 aaggcacttg ctaaaagccc tactaaaaat tggacccatt ctttccgctg ataagaaagt
    14641 ttcatttggt acaatcctaa gaaaagccac gtgaagaaaa tagctgctgt accaagtgtg
    14701 taagcaaagt tataagcact gtcagtgaat aataatgaaa gtaaaaagac attcgtacat
    14761 aacgtcgtca atacgatagc ataagttggt gccccatttt tgttgagttt tccccatgct
    14821 ttaggcaaaa gtccgtcttc agccatgttt cccattgttt caatcggtaa gatcgtccat
    14881 gagagccaaa caccaactaa agaaatgatc aaaccgccgt tgatcacgac tgctccccat
    14941 ttaccaacca tcatttcaaa cacatagccc atagcaggct gcgccttgat cgtttgcagc
    15001 tgattcatcg tcattgtccc atatggaagc atggaaacca agacgtaaat aataatcaag
    15061 ccgaatatcc ctagaatcgt tgctttccca acatctgatt gtttttttgc tcgtgaacct
    15121 acgacagagg ctccttcaat accaacaaat acccaaagca tcaccatgat gcaactcaat
    15181 acttgactgg aaaacgggat cggctgtcca ttcgcattca atataatgtc gctagaaaag
    15241 ttatcaatga attgttggat cgaaaacgtg ctgatagcta ctacaataaa taaaaataat
    15301 ggcactaatt tacagaatgt gacgatagca ttgacgaaac tagcattttc gactccccga
    15361 ttcactagcc atgcatatcc ccataaaaag actgtactca tcacgatcga tgccagattt
    15421 tgcccacctt caaatactgg gaagaaatat cctaatgcgc tcatcaacaa tgttgcaaaa
    15481 gctacgttgc ctaaccagca tgtgagccag taaccccaac cgctgataaa accagcgaaa
    15541 cggccaaaac cttcttctgc gtaactaaag atcccgcctt ttaaatctgg acgtttttta
    15601 ttgatattat tgatcgacaa ggagagcatc aacattgctg ttcctacgag tacccaactg
    15661 aaaatagctg ggccgccagc cgatccggag atgtccgtca taataccaaa tacgccacct
    15721 ccaatcgctg aaccaattac taaggcagca agtggaatta aaccaattcc ttttttctgt
    15781 tgctctgtat ccatgtaagc atcctccaaa ataaaaccgc ttttatcaaa aagtataaca
    15841 aacctttttt atttttactt aattaataaa aactttaata gaaatataat tctaccggct
    15901 tttttctcat tatacaagca aagcgcctag tccctttcca aattacacca ccaaaaaact
    15961 taaaaaagat gctatactaa aaggaaagaa ttggaggatg atccatgaac attttatata
    16021 tatcgatttc aggaaatacg cgtgcctttg ccaaacatct agcagaatat gctgaaaaaa
    16081 tgcatgcaga agatcctgtc aatcctgaag ttactttaaa agaaatccat gaaaatagtg
    16141 attttacgaa agaaacagaa ccatttttta ctttcgttcc tacttattta gacggcggaa
    16201 atggtttaga taacggtgat acagaaatct tgaccgaaac gatgcgtgaa tatctggaac
    16261 atgaagacaa tcataaactt tgtcttggtg tagtcggtag cggaaataaa aacttcaata
    16321 atcagtattg tctaacagcc aaacaatatg cacaaacatt tggctttcct ttcttggcag
    16381 attatgaatt acggggaaca ccatctgatg tgacacgaat ttatcagatt ttagcagaaa
    16441 acaataaata ataaaaaaga agaagctgaa aaatagattt cagtttcttc tttcgtttct
    16501 attgtttcta tcatattaag ctttgacatc catcacaggt gcttttgctt ttgctttacc
    16561 tgttttttcc aagacaaagt tttttacacg atccatattg gttaccaaga cgatcatgga
    16621 tgtttctttg cctgctgctt tgatcgcttc taagtcagct tctgctacaa gcgtatcagg
    16681 tgtcacttgc tgcccttctg ttactttcac atcaaatggc gtaccgccta agtcaactgt
    16741 gttgattccc atatgaagca agatttctaa accatttggc gttgtgatcc caatagcgtg
    16801 tttcgttggg aagacagagc tgactacccc agtaactgga gaatagatat gtccgttttc
    16861 tggttctaca aagtagcctt cacccatcat cttttgtgca aaaactggat cacttgcttt
    16921 atctaaagaa acaactgttc cgtcagcaac tgatgagagg atagtcacac tttcagctga
    16981 agtttgaggt actacttttt caggcacttc atttactgtg gctgtttcag tcgctaattc
    17041 ttctttttct aaacggtctt taggaatacc gaagaagaat gtagcaacga agccaccagc
    17101 ataagctgcc aataatccaa ggatgtagcc taaccattta ccgtcagaga tcaatgggat
    17161 caaagcggct cctgaaggtc caatagcgat agccccgaca tttccgatca ttccgatcac
    17221 tgccccgccg atacctccac cgatacaagc agtaataaat gggcgtccta gaggtaatgt
    17281 tactccgtaa atcaatggtt cgccgattcc taggatcccg actggtaaag ctcctttgat
    17341 catttctgtt aattctttat cttttttgca gcgaatccat aatgccaatg ctgcacccac
    17401 ttgtcctgca ccagccatcg caagaattgg aagcaatagc gtacgaccag tttcatcaat
    17461 catttgcaca tggattggag tcaaaatttg atgcagtccg aacataacca tcggcaagaa
    17521 taatgtgcct agaacgaagc cggcaaatgc tccaccaacg ccaagtaccc agttgatccc
    17581 accgaccatg ccatcagaaa tgaaacctgc taaaggcatg atgaagaaaa tttcaatcag
    17641 accgatggca atcaatgaaa gagttggtgt aacgatgata tctaccgcat caggtactac
    17701 tttatgcagt tttttctcaa cgattgacaa tagccaaaca gcgaagatga cacctaggat
    17761 cccaccttgt ccagctgcaa gagcttcacc agtaaataag tttttcagtg gtgcttccgg
    17821 attcatccct gtcagcatca caacggcccc gataactcca ccaagagttg gtgttgcacc
    17881 aaaaacttgt gcagcattga tacctgtata gataactagg tagctaaaca tcccgttttt
    17941 caagatgttc atcacatcga tatattgttg ccatgttgct gcatcaagtg ttccggctgt
    18001 gactaagttt gctaaaactg cggcaatccc tgccacgatc ccagtaccga caaatgcagg
    18061 aatcattggc acaaagatat tagaaatgtc ttttaatacc gatttgattt tagatggttt
    18121 ttgtttcgat ttttgtgccg ctttcatttc ttgtgcccgt tcgtttacac ggtcttttcc
    18181 agatgctgat gaatttgctg tagctgtact tacagaagaa agtttttctc ctaatttcac
    18241 gcctgccaaa tcaaccattt gctgtgctac tttgttcact ttcccaggtc cgatgatgac
    18301 ttgaagctgt tcatcttcta ctacaccaag cactcctgga atcgctttta attcagagac
    18361 atttacaaga tcatcattga tgatcttcat acgcacacga gtcatgcagt ggacaatgct
    18421 ttctacattg cccatgccgc ctgccccttt ataaatttgt tttgccagtt cttccaaagt
    18481 cagttcgttt tttgccatgg tcttttcccc ctcataaggt cccttttaca aatccgtttg
    18541 cttcagctaa ttttgcttga gcttcctctt tatcactatt tgtcaaaatc atgacgatag
    18601 ccagcttaac attttgatct gcttcttcaa agtacttttc agctgtttcg taatcagctt
    18661 ctgtcgcttg catgatgatt cgttttgagc gttcgattag tttttcattt gttggtttga
    18721 catcgaccat caagttattg aatactttac ctataccaat catactgata gtagaaatca
    18781 tattcaagat aagtttttgt gctgttcctg acttcaatcg agtagagcct gtcaaaaatt
    18841 ctggacctgc atcgacttcg attgggaaat ccgcgtgttt actgatttcg gcttgtttat
    18901 tgcaagaaat cgttcctgtg acagcaccga tcgattttgc gtaatctaat ccaccgatca
    18961 catatggtgt acgaccgcta gctgcgatac caataactat atcatttttt gtcaaattca
    19021 gcttgattag atcttcttgc cctagctcat aagaatcttc ggccccttcg acagctacag
    19081 tcattgcttc ttctccacca gcaattagac ctatgaccat ttccggttct acaccgaatg
    19141 tcggtacgca ttcagcagca tctaacacac ctagtcttcc acttgttccg gctcccatat
    19201 aaatcagacg tccacctttt ttaaaggact caatcgtgtt agcaatcact ggttcgatac
    19261 ttgtcagttc tttttcaacc gcagcggcca ctttctgatc ttctttgttc attagttgaa
    19321 cggcttgaga aatactcatc tcatctaatc caaaagtttc ttggtttctg cgttccgttg
    19381 ttaggttctc taaattgatt ttgctcattt ttatcttcct tcttttctta taaattgata
    19441 gttcatacca gctttgattt ggttgatcaa tggtagctct tctttgataa ctttagctac
    19501 tacattcact ttttcatccg ctggcaaatc atatttggtt aattggatct cacccatata
    19561 gcgcaaatac ttttcgttat ctaaagtaat agaccctttc atacgcgtag ctgtatttct
    19621 tgctgggata ggcggaatct ctcgaaaacg tgcatctacg cttcgtatga catctcttgc
    19681 atcgtcttgc cgattcgtat gcttccctaa tatatattcg taaaattctt catctacagg
    19741 ttccgtatgg agcaagactg tctgttcttt ttggaaagaa gcaaattgct cctgaacgtt
    19801 ttctgacaga cctgcatctc cgatgtaaac aatatccgtg ttacttgcta atagatctag
    19861 agcgcctgca agaggatgga tccctcgatg ttcttccaaa gttggcaatc cttgatatag
    19921 tgggcctctc aactctgtat ctcctggtac aaaaccttgg acaaggagac cttgaatttt
    19981 gagccattga ttttttttct gataccattc tttgtccagc cctgtttcgg gacgtggata
    20041 ataattatgc caagcttcaa tctgtgagaa atcagcttct gcttctttca attcatcgat
    20101 atctttaggc gtgatcgtgc tagcattcaa actgattttc aattgatgtg accagtcggc
    20161 aatctgctga ttggaaatat gataatccat tcttaaacct gttacaccaa ttgctttcaa
    20221 aggcgttaga tcttctaatg aaaaacccgc tcgttccaat gcctctcctg aaatatcgac
    20281 catcaattct agtttattct ttttcgcttc gcttcctagt tcgatcaatc gcttttgata
    20341 tgccgaaaca tcatcttctg gtatatgcat ggaagtgaaa ataccgacaa agccattgga
    20401 tgccatttgg cggatatact gtttcttctc ttctgacaaa gaatcgttca taaatattga
    20461 aaagccaaac atattatccc tctttttctg tatccgtctt catttctatg actagagtat
    20521 atatctaaag aaacaaaatt tcaatacttt tattaaaata attaaatttc atttctaaag
    20581 gttgagttta tattatcaaa agaattgtca taacctaaaa aaggcactgc taagcaaaag
    20641 cagtgccttg aaatatttta tttaaataac acaaaaacat aattactcat aagaaaccaa
    20701 ttctgtatta tctaccatga atgtaaagaa cccctcatta ggcatcggat gaaaataagc
    20761 attgatcact cttatcaagg cattatggca aactaacaga tagacatttt cttcgtcttc
    20821 gatgcattct ttcaataaag gaacgatccg tgcatacaca tctaagactg actccccatt
    20881 tgggaatcgt acagcgaacg ctaaacgtgc tttttgaaag ttctcatctt tgcttggcag
    20941 tccatcataa tccccaaaat ccatttctac caatcgttca tcaacggtca gcggcaagct
    21001 gagacgatca gcaactgctt gtgctgtatc tcttgctcgt tgaagagggg aatggatgat
    21061 tttcgtgatt ggaacaggca aatctactac cttttctgct agtttctgtg cctgcatctg
    21121 tcctttttca gtcagcggca catctgcccg tccgcaaact aatccttgtg cgttccaagt
    21181 tgtttctccg tgtctcgtta cataaagcat gtgattctct ccttcgttgg caaagttccc
    21241 cttaaccata ccttgttgtt cttgagatta caagggttag attctctcaa gagaaataat
    21301 ctcagtagat actttatcca ggaaggtttg atcatgttcc actagtaaca tggcaggttt
    21361 cacgttcaag atcagttgtt ctaattgttc ttgattgaag acatccaaat aattcaatgg
    21421 ttcatcccat gtatatagtt cagctggctg tgataaagat ttagccaatt ccactttttt
    21481 ccgttggccc atactcatct gctcgatctt gttatgaaaa acatctcttt ccatcccaag
    21541 ctttcggagg ttgttcaaaa atgcttggta gtctacttgg tttttctctg caaattccgc
    21601 caacgttcct cgattgtctt cataattttg gcttgcataa ctaatgctca gatgttttgg
    21661 ctggtatttt tctcctataa ccttgccgtt gaatgccccc agaagataat ggataatgga
    21721 tgactttcct gcaccgttag gacctgaaat cgccacccgc tgatgaggct cgattgtaaa
    21781 atgaattggc tcgaataaca gattttcata ccctaattga agatcttcta cgcttaaaag
    21841 tcgcttatgg tgagacgctt ggctattcat cgtcaaagaa tcgatatatt cgatatcttt
    21901 taatagtttt tctttctcgc tgatctgggt ctccatccga ttcacgattg ctttggatcg
    21961 tttcatcgtc cgtgcagcat cagcacccac tgctccttta ttcactcgtc tagattcagt
    22021 atcgatgaat ccgacttgtt tctttgtttt atctccttct cgggaacgag accattcggc
    22081 tttttcagct gctgttttct ttagcctact gacttctttt ttcaattttt cattttgagc
    22141 catttcaaat tcatcacgaa gttttttctg ttcttcatag atagagaaat tcccttgata
    22201 aagttccagt tgactttttt cgattgctaa aacatggtcc actacttcat cgataaatcc
    22261 ccggtcatgg ctgaccacga tgaagccttg ttttttcttt ttcaaataag ccgctacttg
    22321 ttttctacca gagatatcca aatgattcgt tggttcatcg attaacggga aatgagtgtc
    22381 atccacaaat aaaagtgcca gtaggacttt tgtcttctct ccccccgata gtgtgctgaa
    22441 ttctctccat aagatttcag gatctgtttg catcaattgg agctctcttt cgatttccca
    22501 tatctcaaaa tccgtaatat catttaacac gtaataggtt aaacgttctt tatcttttgt
    22561 ctgttgcggg aaataggcaa attcttgctg atggattact tgcccttgat aaggtagttt
    22621 gttttgtaga atattcagta aagttgtctt tcctcgaccg tttcgtccga taagtcctag
    22681 tttccattgt gtgtcaaaat ttagatttgc ttgttcaaat aataatgtgc cttggctgtc
    22741 gtagccgaat gtcagatttt ttatttcgat tttagacatt ttccatcacc tcgtaaaaaa
    22801 agaccagtcc tattttcaga actggctaaa gaggatgcaa gggttggata cacacaaaaa
    22861 gacccataca tcgaaaaaac caagccaatc ccgaaaatct gcacactgct tccttgtctc
    22921 cacaaggtac agtctgaatg cagctttcat caatcgactt aatttttcaa tgcacggatc
    22981 cctcactttg tcctatattt gaaattacga tagcataaag aaaatgattt tgtcaactac
    23041 ttcgggagga tggtgaaaaa tctgctaagt gtctgtccta agatttttgt tttttctaat
    23101 gtttgcctgt gttccattgc ttcttctaaa cctattggac ccaaatgaat acttaagaca
    23161 attgggagaa aatcagaaag tccttctagc tcttggtctc tacttccgca aatagcgatt
    23221 attggttttc gatatttttt tgcaagcact gctacaccgt atggaacttt tccgtggatc
    23281 gtttgctgat cgatccgccc ttctcctgta atgatgacat ctgcttgttc gatctttttt
    23341 tccaatccag tgatttggct gactaaatcg aaaccagaaa ccatcttcgc tcccaacaaa
    23401 aaaagggctc caccaatacc gcctgctgct ccacttcctg gaatatcatc taaatccacc
    23461 tcccagaaat cttttatttg ctgagcaatc tttcctgctt gtttatctaa gaaagctagc
    23521 gtcgtatcta ttcctccttt ttgtcttcca aattgccaag cagctccttg ttgacctgta
    23581 tatggattgg taacatctgc agcgataatc aattttactt tagaaacaag aacgtttctt
    23641 cgatcaaaat gaagtttttt agtagataat aaaggatttc cttctgtgta cccttctaaa
    23701 gaagcaccca aggattggag taatcctaga ccaccatcgc tacacccgct tcctcctaaa
    23761 gtgacaataa tttgttctac ttcttgaagc agtgcatctt taataagttc tcctaaggca
    23821 taactggaag caagtcggat agtttgatcc gttggtttat caagtagatc taaccccaat
    23881 gcttgtgcag attcgatgac tgcggtgttt tttccttgcc aagttgtcaa taaatagctg
    23941 atttttcgct gacggaataa gagatccatc ccatcaatgg tacgccatgt tcccctgagc
    24001 gcgtaataga ttgcttctgc agtattttct ccgccatctg caatcggcac attgattttt
    24061 cttcctttac gatcttccca gccatttaga actgcctgat ttagttcaaa tgaagaagca
    24121 cttcctttaa atgaatcaat tgccgccaaa atatccatct gtatccctca ctttcttctt
    24181 aagtaagaca ctttttttca tttttttcaa acaaaacaat gctattttct tccatttttc
    24241 tatatactaa aagggaactc attgaataaa ggagagaaaa aacatgaaaa tcgcaatagc
    24301 tggggccgga gcaatgggca gccgaatcgg attgatgcta catcaaagcg gaaatgaggt
    24361 cctcttgatc gatcgttggc ctgctcacat tgaagccatt cgtacaaatg ggttgattgc
    24421 tgactttaac ggtaaagaag tggtcgcaaa actgccaatc tattcaccag aagaaatcat
    24481 cgaaagcaac gaacacgtgg acttgatcgt agcattaaca aaagccaatc aattagatga
    24541 tatgttttgt tcgatccaat ccattattac tgacaatact tatgttttgt gtttactaaa
    24601 tggattaggt catgaagatg tactggaaaa atatgtgcca aagaaaaata tcttatttgg
    24661 gatcacgatg tggacagctg gtctagctgg tcccggaaaa gtcacattgt taggtgatgg
    24721 agaaatcgaa ctagagaact tggaaccaga aggcgaagca tttacaaaaa aagttgtaga
    24781 agtctttcaa gaagcaaatt taaatccgat ctatagccat aacgttcgtt attccatttg
    24841 gcgaaaagct tgtgtaaatg gaacattgaa tggtttatgc acgattttgg attgtaatat
    24901 tgctgaacta ggcgcgcaaa aagcggcaga gagcatggtt cgaacaatcg tatctgaatt
    24961 tgcttctatc gcagcaaaag aaggaatcat actagatcaa gaagaagtgt accaacatat
    25021 tgcttctact tatgatcctg acaacatcgg gttgcattac ccatctatgt accaagacct
    25081 gatcaaaaac catcgtttga ctgaaatcga ctacatcaat ggtgccattt ggcgaaaagg
    25141 acaaaaatat gatattgcta caccatattg tgcctttttg acccaattag tccatgcaaa
    25201 agaaggaatc cttggcgcag aataaaaggc tctctgatga gaatcagctg aatcagcagc
    25261 tggttctctt tttttaagcc caataaaaga tcaaaaagaa aacacaactc tcatgatgcc
    25321 ataagaattg tgcttgtttg tttcagtaca taatctcttc aaaatgtagg ttttttattt
    25381 gttttcaaca gaggagaggt catattcgta ttgctcataa atagtggaat cgttagtgat
    25441 aaatcgtttt gcccaatttt ctgcatcata aaattccaat agctctgata ggattggctt
    25501 aaatggtgta tgcagaaatt ccggtaattc atctaaatca cgttctcgaa gcaaaaaaat
    25561 attttcagga cgataaaaat gataatgagc aatcgcttga ttatccgtca ctagtttctt
    25621 ttgaaaatac atggattcaa atgtccgcaa agtcactcct tgctgaccct cccgcaacag
    25681 ttccagaatc ccgcgcgatt cccctgtttt tttcaggtac tcttcataag taagatatcc
    25741 aggttgttca ttccctcgat gtggaagaat gaaatagttg gtcgtaacat ctctttttat
    25801 aaattcctgc ttatagcttt ctgctcgttc ttttctccca ttattcgttg caccaaaaaa
    25861 tagatccgtc tttggttcgc tagaagaaaa attcatatgt ttcgaataaa atgaactatt
    25921 atgtttcagc ccaagttctt ttgcttccaa aaaatcaaaa tgataaatct catcgattgc
    25981 cggttcatct tgtgcctgct tcagcatcgc ctcgtgttct gctttgaagt gattccaaaa
    26041 aaataacaca atttttgcat tggtaaatgg gcgcacgcgt cgagccatct ccatatcaaa
    26101 tccataatct aaaaaaacga tcagctgata atttgccaac tgttgtctag ctgatttaat
    26161 gaattccgac tgtagtaaat cttttccgga ccaaacatct aaagagcgga tggaaggcaa
    26221 aaaataaagt gatttatttt ttgcaactag cagtacgtca tttttgttca tcttttccgc
    26281 ctcactttct ctgtcacttt ttcctatgat agcctgttta aatagtaaaa agccagtaaa
    26341 acgtttccta tatttgtatg aaaatttaac atttgtatga aaatatgatg aaataaatca
    26401 ttcttctatt aaaattcgcc taaaagtttt ctgaatagtt gtaattgtct gaaaatttgt
    26461 tatcattagt cattgttaaa tattgctatt tattcttaat ataaattttg attgtgaatt
    26521 taaaagaaag aggtcattta tggaagataa aaaattgaaa aaacaactta gctccagaca
    26581 tatcaccatg ttagctttag gtggagcaat cggtgcaggt ctatttaaag gaagtggcga
    26641 agcgattggg atcgccggcc cttctgtatt gattgctttt ttgattggtg ggctgatttt
    26701 atatatcgtg atgaaaggtc tgggaaaaat cgttttaagc ggtggcgaca cgcatcacgg
    26761 attatctggt ttgatacgcc cttatctagg tgctcattct gctgatttta ctgattgggt
    26821 ctactggtct atgtggatta tcaatatcat cgctgaagct gttgcagctg cttccttttt
    26881 gcagttgtgg ttcccgcaca ttccagcctg ggtatttgtt tttatgttgg ctgttttaac
    26941 tacgatcatc aatctttatt cggtacggtt attcgcagaa acagaatatt ggctggcttt
    27001 tgctaaaatc agtgtcatta tcttactgat catttttgct acttatcttg tcggtcagca
    27061 aatgttgggc acaggcgttt tccctaccct tcagcaattg actgaccatg gtgggtttac
    27121 tcctcatggt atgaagggaa tcatcagttc tttgcttgta gttatctatt cttacggcgg
    27181 ttctgaactg attgctatca cggtaagcga agcagatgat cctaaacgtg cgattcctaa
    27241 agcaatcaaa ggtgtcatgg gaagaatcat ttccttttac atcattcctt tgtttttgct
    27301 tttgattctt tttccttgga atactttagc aggcacgaat gtcagtcctt ttgtgatggt
    27361 atttgaaaaa atgaatatcc catttgcagc ggatatcgtc aattttgtga ttgtccttgc
    27421 tttgttctca tctatcaatt cgggtgtgta tgcatcttca agaattttgt tcttccgttt
    27481 gaaggatcga aaaggttcct ctagaaaact agctgtttta aacaagcatc aagtaccgca
    27541 acgtgctgtt ttattttgta ccagtgttct ttatcttggt gtagctcttt cttattttgt
    27601 cggtgacaaa ttatttggct atctggcagg ttccctttcg tatacagtat tattgatctg
    27661 gatcattatc agtgcggcat cctttgtact ggcactaaga aaaggaacat tatgggataa
    27721 aggaatcagc ttttttgcat tagctgtctt aggattgatt ttccttggta tcctgttcac
    27781 gaactccatt ggggtaactg tattgactgg cctgctttac ctgttcattt atttcagtta
    27841 tcaaaaaaag aatgatgcgt tcgtattgac gaatgaatga aatcgataga aaattttatt
    27901 ttatttatgt ggaatagatg gagctattga agaaaggagt tgtgacaaaa gtcttgttgc
    27961 aactcctttt tccgaataaa cagtctcatg acctcttggt aaacggtgtt caaaaaccct
    28021 ctgtgcaaaa taattgattg ttctccatgc tcatgacatg atagaagaaa ggaatgaaaa
    28081 aaaggagttt gttttaaatg aaaaagtatt ggaagttttt gttgatgatc ggtgtttcaa
    28141 cggtagttat gtttggtatg atgtacttga atgtctatgc gctcgatcat atcttcttca
    28201 gtcgtacacg agtattcatg gcgttgatga tgggtgcagt catggcagtc atcatgctgt
    28261 tgttcatgtg gaaaatgtat gaaaacaaaa agataaatgt tatgatctta gccgctagtc
    28321 ttcttgtttt tgccggttcc ttgtttatgg tacgaagcca aactactgtc aacgacacag
    28381 cttggatgaa ggccatgatt cctcatcatt ctatcgctat cctggcaagt aaacgagctg
    28441 atttaaaaga tcctgaagta aaaaaattag cggaagaaat cattcaggca caagaagaag
    28501 agatcgacaa aatgaaaaaa atgatcaaag aaatggaatg accattatcc gtccaaataa
    28561 aaaaagagga tgttccagaa gttatcagct tcaagaaata agaagaagct acttctggaa
    28621 cgccgtgaat aaggggttgt gaaaaagctg ccctgcatca agtaataaga acagccaaac
    28681 aaaaatagct tctcctattt ttcgtttggt taggcttatt accgcagatg caagcttttg
    28741 aacaccgttt attaagaggt tgtaaaatca gcgttctgac ctgagtatta gagagacaaa
    28801 tggaaaaata gttcctcata ttttgccatt ttttgagcta ataccgaggg tcagctgatt
    28861 gaacaccgtt tatcgagagg ttgtgaaaaa gtcgcttagt ttcgcgtatt aagaaaaatt
    28921 ttgtgaaatg gctttccaca tttttgtata aatttggctt aataccgaga aactggcttt
    28981 tgaataccgt ttattcgtaa aagggattgt gacaaaagtc ttgtcacaat ccctttttat
    29041 gtttttattg ttctaattgc gctgtcttgc ttactgcttc ttgcggtttc acaaattttt
    29101 ttgagattcc ccaacctgtg aaggcactga ccatcgaaaa cacaggagaa agcaaactga
    29161 aaaatacgaa tggcaagtaa gtgagtgtag gaataccaaa tgtacttgct acgaaacttc
    29221 cggcaattcc ccaaggcact aagtagttga ttactgttcc accatcttct agcgttctac
    29281 ctaatacagt tggatcaaat cctgcttttt cataagtttc tttaaatgca ttaccaggca
    29341 gaataacaga taggaattgt tcaccaataa aaatatttac tccgatactt gtcatcaatg
    29401 ttgtaaaaat caaatttggt gtgttcgtca tctttcttga tactcgttcc atcacggttg
    29461 agatcaagcc aaattcgatc aacaatccac ctaatgacaa agttagaata atcaatgaca
    29521 cagtaggcat catactttcg attcctccgc ggctaagcag tgaatcgatt tgttggtttc
    29581 cagtatttga aacaaaccct gattcgatga ccccagccaa cttcgttaag gaagtttgtg
    29641 ggttttgaat aaaaatcatt cctactgcta cagcgatatt caacaaaatc gttaaaattg
    29701 ccggcacctt tttccatgca cacagtaaca ttaaaaataa aggaatcagt gaccagattg
    29761 aaatagtaaa gtttgcttct agaacatcag tcacttcacg aatagatgaa agacttgcgg
    29821 ctttacttgt gtgtcctaaa atcatgaata aaaagaaaga aacaacaaat gccgggacag
    29881 ttgaccacat catatgcttg atgtgtttga acaaatcggc atcaacaata gctgctgcta
    29941 gatttgttga ttcggataat ggagacatct tatctccaaa aacggcacct gaaacaatag
    30001 ctccaacaac cagcgctgga tttaagccta gtgttgtccc aataccgaaa aatgcaatcc
    30061 caatcgttga cataacagta aaggcactcc ctacagcgct acctacgatt gcacacacaa
    30121 caaatacaga agggacaaac cactggacac tgatcaattt gaacccaatg accattagag
    30181 ttggaatgat ccctgcttgt atccatacag caatcaatga accgactaaa ataaaaataa
    30241 agattgggat gattcctggt ttgatgccat tgatgattcc ttcgtgcagc caatctgtcg
    30301 ggtatctttt tatcactgca tacaacatga ccattccgat cgctgctaag atcgtgatat
    30361 taggtgagac ctttaatccg acaacgccaa tcgcaatgcc taaaacaata accattaata
    30421 caagtgttgc ttctaaaaaa cttggtgttc ctttttttcc catgataatc tctccttttt
    30481 tagttgttct tatgttctaa aaaagttaag atatcggatt actccgccgc aattcatccg
    30541 cgattcctcc tttataaaaa taaaaagacc tgttttaaac taaggacgaa tgactccgcg
    30601 gtaccacctt aattaaaaac agatctgttt ttcactttca aacttttatt caaatgtatg
    30661 gactttccat gtaattcgtc aattaggctt gatcggttca cagcgaccac cgactttctg
    30721 aacaaaacac ctaaccgcta ctgcgtgaaa agttgacttg tacgaagttt accttccata
    30781 agatcatttg tcaatgaaaa agaaaatatt ttttcatttt tctgaaaatt atttttataa
    30841 ctttcacaca caagtttatt ttttactaga aagtagcgcc ctcttgtttt tttcttgcta
    30901 ctatttcttg atgttcttgc ttgattcttt ctaatagctt tggctctttc aacagatcaa
    30961 gagctgtatt ggcgagtagt tctgctgcaa tcgcaataga agcaaggcct ttgtcacttt
    31021 tagcagcagc tttgaattct tctgtgtgtc cggcaatata tgtctcagag attgctaccg
    31081 taggctggat tgttggtatg acttggctga catttcctac atcagaagaa ccgagacttg
    31141 ttctttgttc cgtttctatt tcttcatctg gaacacctgc ttcttttgca tgctggaaga
    31201 ataagtcatc aaaagaagga gtaacgatga catcatcaac tgcattttgg aacaatccaa
    31261 actcaaaagt cgtgcccgta cttatcgcag cacctcgtac gatattttcc actttttcat
    31321 atactttatc taatgtttgc cgatttcctg cacgtaaata gaaacgtcca gcagcgtaat
    31381 ctggaatgac gttggcagct actcctccgt ctgtaatgat cccatggata ttgacatctt
    31441 ttggcaattg caagcgtaga gcgttgattc cattaaacac ttggatcaat gcttccaaag
    31501 catttactcc tttttcaggt gctgctgctg catgggaagc gacaccgtaa aatttgatgt
    31561 cgactggatc attcgctaaa gattctgtcg ttttcccata acgaaaagct ggatggacgc
    31621 ataacgctac atcgacatcc tcaaaaaaac cttctcgaac aaagctgcct ttagctgatc
    31681 cgttctcgcc cccttcttcc cctggggtgc catatacacg gatttctcca ccagtttcat
    31741 caatgaattg cttcactatg ctggcggcta atacggagta agtaccaaac aaattatgcc
    31801 cgcatgcatg accaatacct ggtaaagcat cgaattctgc caaaaaggca attgtgggac
    31861 cgggtttatt ggaaatataa cgtgcatcaa aggctgttcg gtgtccagct acttcttttt
    31921 ctacccaaaa cctttctttt tctaattgtt taatcaacgc tgctgaggaa taaaactcat
    31981 agttacttac ttcaggatgt tcgtggatat ctagagcaag tgcttgatat tctggtaaac
    32041 gatattggat tagatccttc aattggtttg ttgagattgt tgttgtcata aaaaattcct
    32101 cctatgctag tcatttttct tattttctcc cgaatgtttc ccatgcaggc acgcttgatc
    32161 cttttgatgt tttctcgatt actttgactg tgtcttctgt ttgataagcg tctactattt
    32221 tttgatacgt tttattgttt tcatcttctt ttcttgcaac gatgatattc acataaggac
    32281 gtgcatggtc atccactggt tctaagaaga tggcatcaga agcaggatta aatccagcat
    32341 ctacagctac accgctattg atcactgaaa tgtctacatc tgacaaagca cgtgctgttt
    32401 gggaagcatc taattcagaa atgtctagat tttttctatt ctcggtaata tcagaaacag
    32461 tgggcgcttg tccttttgcc ttatccactt tgatcaatcc agcagtttgc aataacagaa
    32521 gagctctgcc gccatttgta acatcattag ggattgctac ttttgctccg tctttgatat
    32581 cagctacatc cttcaaagtg ttagaataaa ttcccagcgg tgcattgaca gtatttccga
    32641 tcgatactaa atcagttccg tgttctttat tgtaattgtc taaaaagaat tgatgttgga
    32701 aagaattcaa atcaatcgat ccatctgcta aagccacatt tggttgattg taatctgtaa
    32761 atttcacgta ttccaaatta atgttctcct ttttcaaacg gtctttcacg ttgtcccata
    32821 catctgtatc ttctccaata atccctagtt ttacgatagt ttcttttttg tcagttgtat
    32881 ctgcttttcc ttggctacac cctgccaacc aaataattgc tacaaatgta atgactacaa
    32941 aacgaataat ttttttcatt ttttctcctc cactttcaat gtgtaatttt tttaatcagt
    33001 tgattactga taaattgact taaaaaaact aagattagaa tcaataatgt agccacaatc
    33061 gttacatctg tttggaatcg attataccct ctcgatatag caagattccc taatccgcct
    33121 gcacctactg ctccagccat ggtagtcagt ccgatcacgt tgatgatagt caacgccgag
    33181 acccgaatga taccaggcaa accttctaac aaatacacac gtaaaatgat accgattgga
    33241 ctggttccca tcgcttctgc cgcttcgatc accccaggat caacttctag cagtgcgttc
    33301 tcgatttgac gagcaaaaaa cggaacaact ccaaaaatca atggcactaa agctgctttc
    33361 gcaccaatcg tcgttccaac taagaaacga gtagtcaatg ctaaaagaga aagcaagata
    33421 atgaaaggga tggaacgaat gatattgatc agcttatcaa gtatttcaaa tagcagacgg
    33481 ttagctagta atccattagg gcgtgtcact actaacgtca cacctaagat gattccaatc
    33541 atcccgccaa tgaatgctgt ccaaaaagac atatatagtg tttgcttcgt cgcttcgaca
    33601 aattcatccg gaatcaaata aacattgggc aaatatgttt taaagaaatc agtcatttta
    33661 tcttcctcct tcgatcactt tgatacgact atctaaatcg tttttttgta aaatatttat
    33721 ttttacacca gatttgatca aataatccaa tgcttttttc cgctgtttgg tttctccagc
    33781 taaagtaacg atcaaactcc ctaacggcgt ttcttgtaat aactctacat tgccatataa
    33841 aatattcgct gttacttgat atttgctgta cagatgagcg attagaggtt cgttcgtctg
    33901 atttccaata taacttaatt ccaccagcca tttattggcg atctgatcgg caaaagcaga
    33961 atgggcaatg atcgtttcta atgcttggtc aagatgagta gctgttcgaa taaaatcttt
    34021 ggtcaattct tcttcaggat tgctgaagat ctggatgctg ctaccttttt caacgattct
    34081 tccatcttcc ataaccgcta ccttgttgca gatttctttg acaacttgca tctcatgagt
    34141 gatcaggacg attgttaaac ctaactggcg attcaatttt tttaacaatg caaggatctg
    34201 aagggtcgtt ttcggatcaa gtgcactggt tgcttcatca cataataata ccttaggttc
    34261 attcgccaaa gctcgtgcga ttgctactcg ttgtttttgt ccccccgaca gttgtttagg
    34321 ataagcagat gctttttctt ccaaaccgac aagttccagt agttcattca ctttttgacg
    34381 acgttcctgt ttcgattttc cagaatactt taaagaaaaa tcaacgttgt caaaaatagt
    34441 tcggctttcc attagattga aatgctgaaa gatcataccg atggcctttc tttcttttcg
    34501 caattctttg ggagagagct gactcagatt cgtctgattg acaatgactt ctcctgctgt
    34561 tggtttctgc agcaaattca tcacacgaac taacgtactt ttccctgcac ctgagtaacc
    34621 gacgattcca taaacatcgc ctttttcaat cgttaaatct acagaatcta ctgcctgtat
    34681 ctctttttct ttttgtttga aacatacaga aacatctcgc aattcaatca ttaaaattcc
    34741 tccttatcta aaaaaagcgt cctcaagaaa atgaaaatgt cattttcttg aggacgcttt
    34801 tttgcgtgtt accacctcat gttcaagctg tgttcacaca caactcctct tcaagtacga
    34861 tcatactcta gcaagatatc ggtcgcagcc gtcatcgcct tacatctatg ctcgggatga
    34921 attgctcgat ggccattttc caatagtcgt tccttgcttc ttctcagcta ccgaagctct
    34981 ctgtcaaagg ttcttctatc gtacttacca tttcaatgca aataatattc aaaaaaaaag
    35041 tccttttact aacataaact atgtcagtaa aaggacgacg tatcgcggta ccaccttttt
    35101 tcggaagttt ccttccctca agcccaacat ctcaaaaaga gagattggcg ttcgttttaa
    35161 cggtcgaaca gttccgaatt tgcctactta tcgacaaatt attctccaag gccattttca
    35221 gcttttctta cttgactctt ctcaccagcc agagcctctc tgcaaagttt ccaaacttac
    35281 tttccttttc aacgaaatcg atattttgtt gttgggatta atctacatga ggaaaactga
    35341 aaagtcaagt acgaaattca aattattttt tctatattta tttttctgtt tttttattta
    35401 acatttcaat aacagttcga agatcttttc cttccgcaag aagtttcaat ttttcctttt
    35461 cttcgacggt ataatttcgg acaaaactgg aatcaggtat caaaacaacg cctaatcctg
    35521 ctcgtgaagc agctgttgct cctactaaag aatcttccac tgcaaaggct gaactagctt
    35581 ctaatcctgt tcgttccaaa gctgtcagat aaggaatcgg tgaaggcttg ttctccgaca
    35641 cttcgtctcc gaatgtaaac gtgtccaagt aatcagctaa tttaaagtga tccaagatat
    35701 ctgttgctct tttcgtaaca gtggtcgtag caatgcctag tttcatatga cgttctttta
    35761 agtctgccaa tatttctttt gcatatggtt ttaagtggat ggctccgctt gtcagctgtt
    35821 gataaaaata ttcttcacgc ttcgctcgga cttcctgtac tttttcaggg ctacctgctg
    35881 ctttcactaa aatatcaaaa ctttgtttcc agttttttcc gccccaagcg tcaacgattt
    35941 cttttgggat atggatatca aattgctgaa atccccaaag ccagccatca cgatacgttt
    36001 tttctgtgtc aactaacaaa ccatctaaat caaatattgc acctttcatc tatacattaa
    36061 tcctttccaa aacaatacac atagtttagc acgactggtc caaaaagaac agaagcaaag
    36121 aaccagcctt ctcgtttata agagttcttg gaatcgacgg atatagattt tcttcacagc
    36181 tgtcacaagc gccagataag cgatgactgt agcaaataac cataaccagt aattgccagg
    36241 aagtggcatc aagcctaatt cagcaccaaa acttgtaaaa ggaaggacag aaccgacaat
    36301 aattcctaat gtagtgataa caaacatggc aaatgttgcg ttgctttgga taaatggtac
    36361 tttcggtgta cgcagtgcat ggagcactaa ggtttgtgtc catagtgatt cgacaaacca
    36421 tcctgcatgg aataatgcga tgaaaatcac tcgctgtgaa ctatccaatg tatggaaact
    36481 tccgcccact acagcaggac aaataacaaa atacatcaac aagtaagtag taatatcaaa
    36541 tatcgaactt gttggaccaa agtaaaccat gaattcccca atactagatg cattccattt
    36601 ttttggctcg tccaaatatt ctttatccat ccgatcccat ggcaaggaaa tgcaggaaac
    36661 atcatagatc agattcaaaa acaaaagttg taaaggcagc attggtaaaa atggcaggaa
    36721 cgtacttgca atcagcacgg aaaacatatt gccaaaatta gaacttgctg tagccttgat
    36781 atatttcatg atattcccaa aagtttctcg tccagaaaga aggcctcttt ccaaaatcgt
    36841 caaatctttt tctaaaagga tcacgtctgc agattctttc gcaatatcca cagctgtatc
    36901 tacagaaata ccgatatcag ccactttcat tgcaggagcg tcattgatac catctcctaa
    36961 aaatccgaca gtatgaccat ttttacgtaa agctgtcgtc aaacgtgctt tttgctgagg
    37021 attcaatttt acgaatactt catggtcttc agctacttgt gctaattcat gatcactcat
    37081 ttccgagatt ttctcaccgg taaccaattc attgatctct agacctactt gacggcacac
    37141 tgaacgtgta actaattcgt tgtccccagt cagaactttt acagctacac catgttcttt
    37201 cagagctttc aacgcttgtt tcgttgtttc ttttggtgga tcaaggaaag caagataacc
    37261 aatcaaaacc atgtctgact catctttgac tgaaaattct ccaacgacgc ttggattggt
    37321 cttttgtgcg acaccaatca ctcgtaagcc atcttcatta agtgactgta cttttgcaag
    37381 tacggcttct tttcgttctt ttgttaatgg tgtaacagta ccgccaagat caataaattt
    37441 agaaatgttc agcatttctt caatcgcgcc ttttgtgatc atttgtgttt taccagctgg
    37501 atcagcaacc acaacactca tacgacgacg ttcaaaatca aaaggaattt catccacttt
    37561 ttgataattg atcttatctg tatctaaagt ttctgttgct tcatcaatga tagccacatc
    37621 taacaaattt ttcagccctg tttgatagta gctatttaga tacgcatggc gcaaaacacg
    37681 atcatcttca tttccttcac aatccatatg atattcaaga atgattttat cttgtgtcaa
    37741 tgtccccgtt ttatccgtac ataacacatc gattgcacca aagttctgga tggcattcaa
    37801 atttttgatc actgttcctt ttttggccat cacacttgct cctttgacca aattcgtcgt
    37861 aacgatcatt ggaagcattt caggtgttag accaactgct acagatagtc caaaaagaaa
    37921 agcttctagc caatcccctt ttgtaagccc attgatgacg ataactgctg gagccatcaa
    37981 tgacataaat ttgatcagta gcatggatgt tttatggatg cctaattcaa agcttgattg
    38041 aattggtttt tcagatagcg tttctgctac atgaccaaat agtgtttcat ttccaacaga
    38101 aacaatgatt ccttccgcac ttccactgat gacattactt cccataaaga caagattttc
    38161 gtaacttgtt tcgctttgct gatcgtcttc acatactttc gcatgctttt ctactggata
    38221 gctttcccct gtcaatgctg cttgggaaat gaataaatcc tttgcggaca ataaacgcat
    38281 atccgctggt atcatatccc ctgctgaaag ccgaacaaga tctccacaga cgatttcttc
    38341 cataggaatc tctgtgtact ttccattacg ttttacagct gccgttactt tcacaagtga
    38401 tttcagtttt tcagccgctt gattggaacg tacggactga atcaacgtca ttgtcccgct
    38461 gacgataacc atagtgaaga taatggctac accgaaaaga tctttatctc caggagctgc
    38521 taatacatat tccgtgatga atgaaataat tcctaatgcg atcaacacta atgtaaatgg
    38581 cgtgatgtat gctttcaata tttctacaat gaatggtgtt tttttgccat atgaaatcgc
    38641 attttcaccg taattttctc ttgaacgttc gatttgttct tctgtcaatc catttgctga
    38701 tgcatggaat agttcaaata attcttgttt cgtttttcca gcaaaagctg catagatttt
    38761 ttgtgtatct ttcttttttt ccatctcaat ctctcctttg tgtccaaaag acagctgttc
    38821 tcgctgcttt tggtctttag ctattcatcg gatcgataga gaaaaaactg aaaggggctg
    38881 agaacagata gataatagcc ctggcctttc ctctagatcc ttgtttttcg aatgactgtc
    38941 atccatttgt tctcatctcc tttactaagt aattttaatt catgacttcc aatcattttt
    39001 cgaatagtta tttttccaat tagaccacct cctttttatt tttcaccgat ggggatttgc
    39061 ggcaacaaaa aaacctctcg ccaaatactc agcgaaaggt ttgaaaaagc atattaaaaa
    39121 aagcttctca gatctcatcg ttgagttttg acactgtaca acgtaaaaag cagatacctg
    39181 cttttagcta ccttatgaaa agccttatct cgacaattcc tgttctaccc attggcgtct
    39241 ctcgacgttt ttgggcagta gcctatgttt gtataggagc ctcacctaac aaatcttatt
    39301 cggttttcct tcgttaatat aacaactaaa aaagaggatg tcaaatcaat tttccatgtt
    39361 tttcatactt tttttaaaat acacacgtta tattcctgtc tcacgcggtt ttatttgtct
    39421 cattttccac ctcccgctat aatagaaata tagtaggaaa cgaggtgctg ttatggaaag
    39481 aacacttgtt attatcaagc cagacggcgt gagacgccat ttagtcggaa gcatcatcca
    39541 acgttttgaa gcaaagggac tggccattgc tgaaatgaag tttgaaacta tgactcctga
    39601 attagcaaaa gaacattatc agcacttgac tgagcgcagt ttttttgatg aactgatcga
    39661 ttacatgact tccggtcctg tcgtttatct tgtgctggtc ggcgaggaag tgatcgatat
    39721 cgtccgtaag atggttggag caacaaaagc tgccgatgct gttccaggta cgatccgagg
    39781 agactacgcg ctgccgggga ctgaaaacat cattcacgct tctgattccc gtgatgctgc
    39841 ggtaaaagag atcgcccgct ttttcccgcc attcgatata aacaaaccgt ctgaaaaagc
    39901 ggcgaagtga tacaaattct aaactgctgg tctagttgct gggttcagca atggccagca
    39961 gttttttctc taaggacctc cgttgattct ttcctaacat atattcgagc actgtgaaga
    40021 attcttggta gtctttcgtc ttttcttccc ctaaagcagc agaaatcaat ctttgataat
    40081 aatctgccat caatgtttca tagatcactg tacttctttc tttatgtgat aagctccgtt
    40141 tcatatcttc attcacacta cgtgcctttt tcaaccgacc attttctata taaaacgaag
    40201 tcaggttcat taaaaaagta aagagatact gctcatgatt gttcaactgt gctaatcgtc
    40261 gcattctttt tttgattttc aaataattgt tctcaatgaa ccgatcactg aatatccaag
    40321 gaaaatcagc tagaatagat agttcaaatt gaccatagtc tggtatctgc atcaagtatt
    40381 cttcaatgat tcggcattca taacgtaaaa attcaaattc tgtctcagat acttctgttt
    40441 tttcgaccat gtgaagattc aaaaccaaat aatacagatc ttctgttttc ttaaaatgcc
    40501 tacggttatt gctgatctct ttataaggat caaagctgtc ttcttgtacc cgatctagca
    40561 ttcgtctttt tttctttaac tttattggtt catccagctg gtttcgcatc cattcatatt
    40621 ctgcaagtgt catgttcatt cgctctaaaa atgatctcaa tgttacaaat gacatttttt
    40681 tcactgaatt tgtctcaatc ccgactagag aagtcttaaa acagattcct tccgctaatt
    40741 gggactgtgt caacttcttg ttgactcgaa gtttcttgat caattcacca tcatacataa
    40801 tagttctcct ttttaattta aatttttcca cattcaataa atagatacgt taaaaaggaa
    40861 aaatacacga aaaaaataaa aaaatgtttt tttcataaaa aataccaacc ttcaagcatt
    40921 agaatgttgt ctaactactg aaggttggtt tgattgttta ttgattattt ttttgctgtt
    40981 caacgtctgt tcttgttact ggatataagt tggttgttgt tttattacca ttttttgaaa
    41041 agacacttgt cgaatgttcg cctttttgct tttcgattgc ttcattacgt tccgcttcat
    41101 ttttatagtc atattttttc ggatcaatcg tctcaaatcc gtttggcaca taaaactgca
    41161 agagattttc ctgattcagc ttatctgaaa gacttaacgc agtcttgact ttttcctgat
    41221 ctttggagat ttcctcatcg attcctgctg ctttcgtatc gatgatttct cctgtctgat
    41281 tattgtaagc ttttcctcct aggacggtgt atttgggtgt cacaaagttc ccatttcgaa
    41341 aagcaactac ttgatcatgc tgagaagata ataagtctgt accaaaatga agataatctt
    41401 tatcatcgat tcccaataga tgtagtaatg tcggcaaaac atcgatttct ccaccgtatt
    41461 catgattgac tgtcccttta gtataaccag gcatatggat catcaatggc actcgttgca
    41521 tctgtgcgtt gtcaaattca tcccatgtat ctggatcttt accaagagcg cttgctaaat
    41581 ctttattatc tgtattcgag atcccaaaat gatccccgta aatcacaaag attgaatttt
    41641 ggtaaatacc agattttttc aaatacgtaa aaaattgttc caatgattga tccagataat
    41701 gtgccgttcg aacataatta tctactgtgc tattgccagt attcaaagat ggaaagtcga
    41761 aattcttgtc atccgtataa taaggcgtgt ggttggttac ggaaagaaat ttcgtgtaaa
    41821 acggctgttg tagatgctca agatattggg cagattctct gaataaatct ttatctaata
    41881 tcccataacc taacgtttcg tcggattcat caaaataaga tcgatcaaag aaattgtcat
    41941 aacctaagtt tttatataca tgatctctat tccaaaaact agctacattc ccatggaaaa
    42001 cagcgcttgt atagccttgc ttttgtccaa ggatcgctgg tgcggcttga aagacattgt
    42061 ctgacccaag ttctgtgaat aatgagcctt gaggtaaacc aaatgtgcca gtttccaaca
    42121 tattttcagc atcacttgtc tttccctgtc ctacttcatg aaagaaattg tcaaagctga
    42181 tcgttgcttg attctggaaa atactattta aaaacggtgt gacttcttgt ccgtctacct
    42241 tcatattaat caaaaactgc tggaaacttt ctaaatggag aacgatcaca tttttccctt
    42301 tagcgatccc aaatgtttcg ggatttggct ctgcataatg tttctttgta taatctaaaa
    42361 cttcatcaat cccattactg cttgcatgcg cgcgaacact gctagtcatt cctgttttga
    42421 ttccgtcata aatagtatac gcatcgattc ctagatattt gacaatatat gaacggtcaa
    42481 atgttcgctg cagcaattgt ggacggttgg cttcgctcag cgctagattt cctgaaaaaa
    42541 taagccctga aagacaggta acagcgatag ccatcggttt gccgacaggg ttgcttttga
    42601 taggtatttt cttgaccttc aaccaaacca gtagcccaat gaacacagcg atatccagcc
    42661 agtagatgac atcatggggc ttcatcaaag aaaaggagct tcctgacaat ccttgcgaca
    42721 ctttcgaaaa gcctaataca gatttgactg taatgaaatc tgtgaattcc cgataaaaaa
    42781 taatattcaa gtacaacaaa agcgtattta aaatatcaat gatcaaaaga atagctaacg
    42841 ctggctttgg ctttttaata tacaaagaaa ggccaaaaaa caataaggta gtcgcaattg
    42901 ggttgatcca caagataaag tactggatcg taccttccac acctaatgaa aaatctgcat
    42961 agtaggcaat gatcgtcttc agccagaaaa agaaaacagc tgtcaaaacg attgctaaac
    43021 gattttggca caatttttta agttgtgtca cgtatatcac ttctctctat tcacattttt
    43081 tcttattttg ctcctgaaaa atgaaaattt cctaaaaaag ttgttatctt aagatttttt
    43141 tattctttct aaagattatt ccgtccactt ttcacagcat aaaaccgctg cgatgtaagc
    43201 gattaaacta atgaagagat aaaagtcttg tggtgaccgc ttcactccac ggtattcaaa
    43261 agccagtttc tcggtattcg tacaaaaaaa gaggctgtga caaaattctt gtcgcagctc
    43321 cttatttctt gatgcaggac agctttttca caacttcttc cttaaacggt gttctcctgc
    43381 caactcctcg gccacaagtc acaatattta taaaacatga gaagctgttt tttgaaattg
    43441 ttccttgtgg ctcagagtta aacggcaggc tcaagacctc ttgatacacg gtgttcaatc
    43501 agctgaccct cggtattaga ttatgtcagg acgctaattt tacaacctct tggtaaacgg
    43561 tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat aaggagctat
    43621 tttttgaaat tgctccttgt ggctcagagt taaacggcag gctcaagacc tcttgata
//
LOCUS       DACCCS010000017        43404 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.017, whole genome shotgun sequence.
ACCESSION   DACCCS010000017 DACCCS010000000
VERSION     DACCCS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.017"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..209)
                     /locus_tag="IVK82_000997"
     CDS             complement(<1..209)
                     /locus_tag="IVK82_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6392073.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSD"
     gene            complement(308..622)
                     /locus_tag="IVK82_000998"
     CDS             complement(308..622)
                     /locus_tag="IVK82_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392074.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(690..1184)
                     /locus_tag="IVK82_000999"
     CDS             complement(690..1184)
                     /locus_tag="IVK82_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392075.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(1177..1491)
                     /locus_tag="IVK82_001000"
     CDS             complement(1177..1491)
                     /locus_tag="IVK82_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392076.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            complement(1707..3098)
                     /locus_tag="IVK82_001001"
     CDS             complement(1707..3098)
                     /locus_tag="IVK82_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP6392077.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            complement(3083..3694)
                     /gene="mobC"
                     /locus_tag="IVK82_001002"
     CDS             complement(3083..3694)
                     /gene="mobC"
                     /locus_tag="IVK82_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP6392078.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(3698..3895)
                     /locus_tag="IVK82_001003"
     CDS             complement(3698..3895)
                     /locus_tag="IVK82_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392079.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            complement(3886..6564)
                     /locus_tag="IVK82_001004"
     CDS             complement(3886..6564)
                     /locus_tag="IVK82_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392080.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            complement(6551..6769)
                     /locus_tag="IVK82_001005"
     CDS             complement(6551..6769)
                     /locus_tag="IVK82_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392081.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            complement(6845..7897)
                     /locus_tag="IVK82_001006"
     CDS             complement(6845..7897)
                     /locus_tag="IVK82_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP6392082.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDNSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            complement(8050..8376)
                     /locus_tag="IVK82_001007"
     CDS             complement(8050..8376)
                     /locus_tag="IVK82_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392083.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(8388..8588)
                     /locus_tag="IVK82_001008"
     CDS             complement(8388..8588)
                     /locus_tag="IVK82_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6392084.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            8663..9313
                     /locus_tag="IVK82_001009"
     CDS             8663..9313
                     /locus_tag="IVK82_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6392085.1"
                     /translation="MSIKILLNLWFSCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            9368..10534
                     /locus_tag="IVK82_001010"
     CDS             9368..10534
                     /locus_tag="IVK82_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6392086.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(10623..11015)
                     /gene="rpsI"
                     /locus_tag="IVK82_001011"
     CDS             complement(10623..11015)
                     /gene="rpsI"
                     /locus_tag="IVK82_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP6392087.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(11031..11474)
                     /gene="rplM"
                     /locus_tag="IVK82_001012"
     CDS             complement(11031..11474)
                     /gene="rplM"
                     /locus_tag="IVK82_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP6392088.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(11993..12460)
                     /locus_tag="IVK82_001013"
     CDS             complement(11993..12460)
                     /locus_tag="IVK82_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP6392089.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(12580..14118)
                     /locus_tag="IVK82_001014"
     CDS             complement(12580..14118)
                     /locus_tag="IVK82_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP6392090.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            14198..14884
                     /locus_tag="IVK82_001015"
     CDS             14198..14884
                     /locus_tag="IVK82_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP6392091.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(15085..15612)
                     /gene="msrA"
                     /locus_tag="IVK82_001016"
     CDS             complement(15085..15612)
                     /gene="msrA"
                     /locus_tag="IVK82_001016"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6392092.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(15696..16373)
                     /gene="pgmB"
                     /locus_tag="IVK82_001017"
     CDS             complement(15696..16373)
                     /gene="pgmB"
                     /locus_tag="IVK82_001017"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP6392093.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(16370..17500)
                     /locus_tag="IVK82_001018"
     CDS             complement(16370..17500)
                     /locus_tag="IVK82_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6392094.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(17500..19683)
                     /locus_tag="IVK82_001019"
     CDS             complement(17500..19683)
                     /locus_tag="IVK82_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP6392095.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(19680..20705)
                     /locus_tag="IVK82_001020"
     CDS             complement(19680..20705)
                     /locus_tag="IVK82_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6392096.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(20750..21577)
                     /locus_tag="IVK82_001021"
     CDS             complement(20750..21577)
                     /locus_tag="IVK82_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP6392097.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(21574..22641)
                     /locus_tag="IVK82_001022"
     CDS             complement(21574..22641)
                     /locus_tag="IVK82_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP6392098.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(22667..23503)
                     /locus_tag="IVK82_001023"
     CDS             complement(22667..23503)
                     /locus_tag="IVK82_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6392099.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(23500..24369)
                     /locus_tag="IVK82_001024"
     CDS             complement(23500..24369)
                     /locus_tag="IVK82_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6392100.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(24439..25722)
                     /locus_tag="IVK82_001025"
     CDS             complement(24439..25722)
                     /locus_tag="IVK82_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP6392101.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(25736..27421)
                     /locus_tag="IVK82_001026"
     CDS             complement(25736..27421)
                     /locus_tag="IVK82_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP6392102.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            27696..28721
                     /locus_tag="IVK82_001027"
     CDS             27696..28721
                     /locus_tag="IVK82_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6392103.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(29316..32969)
                     /gene="rpoC"
                     /locus_tag="IVK82_001028"
     CDS             complement(29316..32969)
                     /gene="rpoC"
                     /locus_tag="IVK82_001028"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP6392104.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(33048..36665)
                     /gene="rpoB"
                     /locus_tag="IVK82_001029"
     CDS             complement(33048..36665)
                     /gene="rpoB"
                     /locus_tag="IVK82_001029"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP6392105.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            37393..38361
                     /locus_tag="IVK82_001030"
     CDS             37393..38361
                     /locus_tag="IVK82_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6392106.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            38379..39284
                     /locus_tag="IVK82_001031"
     CDS             38379..39284
                     /locus_tag="IVK82_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6392107.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            39281..40087
                     /locus_tag="IVK82_001032"
     CDS             39281..40087
                     /locus_tag="IVK82_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6392108.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            40244..41212
                     /locus_tag="IVK82_001033"
     CDS             40244..41212
                     /locus_tag="IVK82_001033"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP6392109.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(41529..42053)
                     /locus_tag="IVK82_001034"
     CDS             complement(41529..42053)
                     /locus_tag="IVK82_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP6392110.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            42340..>43404
                     /gene="scm"
                     /locus_tag="IVK82_001035"
     CDS             42340..>43404
                     /gene="scm"
                     /locus_tag="IVK82_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP6392111.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        12443 a         9023 c         7633 g        14305 t
ORIGIN      
        1 aaatcagata cataagccca tgtacccgtt cgttgtaaat aaagttcttc ttgtaacaac
       61 tcccattttc tcttagaaat cataatgacg ccatcgttca atccattttt agaatcccta
      121 gtaatttcta tttcagaatt tgaatgaact acttcttgca acactttacc tatattttct
      181 ttgaagtttg ttggagtaat agtatccaaa atgaatacct ctcttctcta tacctctatt
      241 atgaagtgcc cataaaataa agcaagcgtt gaggaataat tctatcaaca cttgctttat
      301 taagctttca ttttgtagtt tgactaaaac caataatctt atttctaaac tctcgaaaaa
      361 cttcttgtgg ttgataatag tctggatcta tttcaaatgt ttgtgagaat aattcattga
      421 atttttctac tgaaataatg attttaccat tcttctctcg actgttttct attatttctg
      481 cttctaattc aaataaatct agtgacgaac taaaatcata aatcatgtca tattgaataa
      541 aaaaatcaag aaaaagttgc tcaaactctt ccttatttat cactaatgtt tcaaacggaa
      601 caactttatt ttcctcagcc attctcccag ttccattttc aaaaatcaca aaactgatat
      661 taccttaaaa actactaaca aaataaaact tataaagtaa atggcaaccc aactatgtaa
      721 ccattctctc tacgtactac gatttggtag tcctgccttt caatcggtaa ttcataatag
      781 taatttactg tttcttcttt gccactttca atatactctg gattatccaa tatcatatca
      841 tactcataaa gttcttcacc atccttattt tcaatcgacc attgttgtgg acgtatcgga
      901 ttttctgtat catttataag tgtataggtc acttgtaata cacgatcatg ttgtgcaaat
      961 tcatttcttt tttcggtcac ttcaatattt ttaaatgtta gttcaacccc atctgcctga
     1021 taagtttcac ctatttttaa atcttttgtt acaattttta ttggcttttc ttctggaaaa
     1081 tgtctggctt taattgttag ataacttcca aaaagaatca atcctagtag cactaaaaga
     1141 attcctttaa cgagccactg aaacgggaag taaactttaa acatcttttt tctcctccgt
     1201 tttgtatttg ttttttagag aatacgataa cttttgatca atgtcatcca gcttttcaat
     1261 tacttcatta cttatcattg attctagttc tgaaattttt tgttcaaatt taatgctttc
     1321 aggatcatta aatttattat ttgatggact agctaacact ggtaaatttg aaaccataac
     1381 acgaaacata tttgcctttg aaccaaaaat tcctttgttt tggttgtaaa aattgttgaa
     1441 tatttcttta gtttcttctg aagccgagac tttaaaatat ggatagttca tttttttctt
     1501 ccacctttct ttttttggga gggagtctgt agggagcttg ctccctcaga gatttacccg
     1561 ccatctgact tgccagaaag tagcctagcg tgagcggggc ggattgactg gcaagtagat
     1621 gggtagctgg cgtatgtcct aaaaactgct tctttccaag caactcttca taggcaaaaa
     1681 actggaaagt ccactaacaa gcccattcag aattccattt caaaattttg ttgccttttt
     1741 tgctgatttt caatcatttg ttgtgtgaat tcataatcct ttttagcacg atacattttt
     1801 acatcgtcat tcattatgcg actgatttga tagtgaatct gtttagcaga aggtgtatga
     1861 gaacaatttt caaaaccctt tgccggtaga aataactctt tctgatcagt ctttttaata
     1921 gaaattgttt tacttttcgc actacgtaaa gtttgaggat tcacatccgt atgaagttta
     1981 atcgtgtatt cttgatcatc ttctaaagac attgtattta caaaatgctt gtctacctct
     2041 cgcaagaagt catcatattt aatgaatttt tcatcgcctt cacggtcgct aaattgcttt
     2101 tgtgcgatgg aatctaattc cacaaacggt acatttaact caccagtttt gtaatttact
     2161 ccactaatta gcgacataac ttgctctctt tccatattaa aaaaattttt tatatgtgat
     2221 tcttctaaat tcttttcaag ttgttcagaa acagattcaa atgaatatct tttccctaac
     2281 tttcttgcac taaaagtacg ttcgacatac tgactttttt cttttctaga ttgtttatct
     2341 cttcctaaag taattaactc acctctacgc tcatagaaat ttacaccttg tttgtaaaga
     2401 acatagccaa gtttgttatt tgtttttgaa aattgataaa cttcaatgtt atattctttt
     2461 gccaaatgat ccgaaaaatc ttcaaaagaa ttcacttcgc atttttccaa cgttgcttcg
     2521 atcgcatttt gcacaaactc ttgataatta atttttccat atttcatctt agaaggatcg
     2581 tttgaataaa gattggttct cttttctaag ttttcatagt actcattatt cactgattca
     2641 agaccatgtt cattgaggac ttgatcgtta atttttcgca ttagttctaa atctttaggg
     2701 tcaatacgca accttttaca tgtttccatg ttaatattat taatgacaag atgactatga
     2761 aaatgatctt tatcattgtg acttgcaaca atgatttgcg aatttggaaa agctttctct
     2821 gcaaattcga cagctatttc gtgcatttta tcaatactta actctttaga agtactctta
     2881 tcaaacgaat gaataacatg aaacgcttgt gctatatctt tagatttatt catcttggta
     2941 cgtactaaat tcatttcttg cagtgcttcc tcactatcat cactacaatt tattcctgag
     3001 actgcaacac atttttcttt ttttgaagga ttagctgcat attgtaatag cgaggataaa
     3061 tttttcttgc gtcctttcga tgttatagta gctcccaaag ttgtttcacc tcatctttta
     3121 aacttgcaat aatctgaatt gagtgaatta aattccgatc aactttttgg gaatactctt
     3181 ctttcgtacc cggtctagtt tgcttttcta tccaatattc gaaatattct ttttctactt
     3241 ctgataattt aaaaaaagag ggggcactcc gattggactc caaaaaaatg tcatctggat
     3301 aactaatcat caactttttt gctatgctat tcgcaagttc atctttaaaa atgttgtaac
     3361 ggctagcatc aaaactaaaa tgtaggctct tttctttttt atcttcagat tcttctctaa
     3421 aatatttagc aatgatattt aatgccttta ccgcttgatt caaattgtta ccaatttgat
     3481 ttaactgttt ggttaaaact accctatcat cttcagctaa caacggcggt tcatatttga
     3541 gtccctttaa ccgctcacgg caaacgtctg ctctagaaat tcccctttgt tttgcaatca
     3601 atgtcagttc aagataagtc tttttatcga cacgaaaatt taaggcctca ctatgtttgc
     3661 gatttttttc gtacattttt tgtttatcat ccattgatta tcccctcaca tttttttcta
     3721 gagagcaaca tttgaaattg ctctctattt catttttcat ctttaattct tcttgaatca
     3781 tcagttcaat ataattagtg accgtgattt tttcatttgt tgcgttcttt actgattcac
     3841 ataattcgtc aagttctaag aaatcatgcg tactaatctt tagaattaat tgcatgacaa
     3901 tccccccctt ttatttctta ataaattatc gaaaagtgga ttaggatcat taagtgtatc
     3961 tctgaataac tcattgtctt cataagtgaa tcttcgtctt gattttgatc ccttatcatt
     4021 ttcacggatt tcactatcaa taatcactgt ttcatgattt gaatcaaatt ttttcgctga
     4081 ctttctgtct tcaacgatat tgattttttg tgttttttct ttcatttctc cactatcttt
     4141 ttgaatgttc tcccgaacat ctactgtttt gaatccttga ctacttgtag aactttgtac
     4201 attttctcgg acatctactg tttttggttg acttgcactg ctaccttgca cattctctcg
     4261 gacatccact gatttaaacc cttgactact tgtagaattt tgtacatttt ctcgaacatc
     4321 tactgttttt ggttgacttg tactgctact ttgcagattt tctcgaacat caattgtctt
     4381 agtaccttga gcatttgaag aactttgtaa attctctctt acatcaacat tccgtccaac
     4441 atgaccattc agtgaacctt gaatattttc tcggacatct actgatcggc caccatgact
     4501 acttgtagaa ttttgtggat tttctttgac taatttttct aatcctgtat ttgaattttt
     4561 agcttcttga attctttctt tggtcaacgc ttcttttccg tcaatattag cgcctgtccg
     4621 cccttctaca cgtttcctta acattatttc ctcgcgattc attgcttcag gcgtgtattt
     4681 cgcttcatga cgcttctgat taattaattc ttcagcagac ttagcttgac cgtgcatagc
     4741 atttttacgt tcttcaatat cttgttggac tttttgctga attgcgcttg caggattatt
     4801 attcgattca gcattatttt tcattgcttc acgcatacgt tcagaactag acataattct
     4861 atcggcattt tcaactttag caccatcttt tctatctgaa agacgtgttg gcacggaatc
     4921 agcatatttt ccagtttcat cttctttatt tttagctaac ataggtttgc tatttttatt
     4981 aatagcggac actgtaccat catctaaacc ttctttaaac ttattcttgg tatcattagc
     5041 tatatcacgc atactcttaa atggttttgt tgctctttca ctagccattg tagctccatc
     5101 tgaagctaaa ccggataatc cacgttcatg ggcatatccc aaagctcttc cagttttgcc
     5161 agcttttttt gatagagttt cgccgaaatt cttttctggt tttcgatctt ctccttctcc
     5221 gcttccttta gcacgattaa atccatttga tccttttcta aacagcattg cgcccattcc
     5281 aaaagcagaa gcaagttgcc catagccttc tttgagtcca atatcaacac caaagtatct
     5341 catgactgtt ccactgcctt tgattaaaac aaaaacggca cagatataag caattgtttt
     5401 tataaaaata tttgttgaga taccttgtcc tgagttcaga aaattgataa acatagcaaa
     5461 cattgataac ccaaatcctt gaaacccaac agttaagtaa cattgtagaa tatctgatag
     5521 aacaactttt gaacgctgtc ctgtctcaat atctgttgca aaaacaagaa cacctaacac
     5581 acgtttaaat gcaagttcta gtattgctgt aatgatcaca aacgcgctga acacataggc
     5641 gattgctaac gctgtcaacc caattgaaat cccccataga tttgcttggt atcggtaata
     5701 gcctgatttc aagctatcag aaaatgttga taagaagttg tcagaaaact ttgtagcaac
     5761 aaattcatta ttagaatttt caactaattc ataacgaaga ttgtccgcaa ttggattttc
     5821 tttttccaat ttatcaatca ctttatccgt taacacttgt gtgagatccg tctgttgaaa
     5881 attttttcgt gttaaattat ttcgatcctc cgttttatca atgcttgagt actgatctgt
     5941 tttattaata tatgctaaat ctgttactcc ttgttttatc aatgaccaag cgactccgtc
     6001 atctgaattt gtaatcgtag tagagtcctt ataaaatgtt ttagcaaatt gaataccact
     6061 agaaatcatg gttggcatta aaagtattaa tgctatagac atcactacgt tcattccaac
     6121 cgattttaaa tccactgttc cttttccagt aatcattttg taaccaacaa acatcaatga
     6181 aaggatcatt aatccaatga caattgtatt tacggctgat tgataaacat tatttagccc
     6241 tgtactttca atgaaattga acaaatccag cacatctgga atcatatttt gaatcgaatc
     6301 tgtcacagta aacatgcctt ttaccacagc ccatccgagc caaatcccga actgactaag
     6361 gaaatctcca ttgtactttt ttaggtaagg tctataaaga gaattattca aaaactctgt
     6421 ttgacttttt gtgtcatcaa accaattgaa aatactcgca tgagcaatat tacttgtaaa
     6481 aataatcata gctatcagaa caagaaaatt aacaaccaac caatttattt ttttattggc
     6541 aatctttttt tcaattattt tcatcgttac ctcccttagc ccattttctg aggctctcag
     6601 agagcttctg agccattttt tgattaatta tgacatgagt tacacaatta tcattttcag
     6661 ctcttaaatc gagaactata gtgttgaagc aaatagtctc gattaattcg agactatctg
     6721 tattttgatc tagacaccaa acttgatcta ttaaacgatt tttaatcaaa ataacccctc
     6781 ctatattcaa agttctcaaa tgaagtggag atacccctcc atttcatttg actcaattct
     6841 attcttattg cttatctatt ccttcgctga tttgcaattc aattagttct aacgctttat
     6901 tttttagaga agcaaagata taatcgtctt tttgtttgat acgatgatct gtttttgctc
     6961 ttcgtatcaa gtcacgtaaa gtcattgata gctgttctct aacatgatct acaaaatact
     7021 tgttattaca aatttcatag ataattttca gttgaatttt ttgttctttt tcagcagatc
     7081 taatcgcata tttgaatttt ttgtaccagt tgtcgaattg acgataatca ctattgctag
     7141 ccagttttaa gcaattcaca atactttctc cccatatttg tttaagaaga ttttcttcaa
     7201 taaatgtttc gatcaattct tcatctaatt tattattttt agttgaaagt gattcaagaa
     7261 gatcatcatt atgtgattct ttaatatctt tactatcttt ataagactta tataattcta
     7321 gttcattttt tgaactatca gcaaaattgt ttttgtctct tgttttcatt gatgtgtcgc
     7381 atagttcaaa ttttgaatta tcaacagaat attgatttgt tccatagttc aaattttgaa
     7441 ctatcaagtt tttttgtcta ttttcttgat gggttatctt attaatttta taaacatcat
     7501 ctttgcacag aacaggcttt aacaaataat aaaaatttgg gaacatttgc attttacttt
     7561 tattgaagcc attttttctt ttcacaagaa gtttaacttt ttgaagttca tctagtgctg
     7621 ataagattgt attttttgac attcccgaaa tttctacgag atttttgatt tcaaatatca
     7681 aaaaaatttt ttgattctca tcatacaagt tatttttcaa tgaaagtcta tttctatctt
     7741 ttagtaacat ataaactaac ttagaactta gtttcatttt cttatatttt tcatttacgt
     7801 ttaaaaaaat aggatattga tagtataatt ctgcatatat atcagaatta gaatagcctt
     7861 gcttttcaac taagctttca acttcttttt cattcattgt cacacctgct tctgatttta
     7921 tttgctataa tagattcaaa taaagttttc tcagaacttt attattgagt gttcccccga
     7981 tctgcaaact gacgggaaca ctctttttaa ttttttctac ttcatcaaat ttaaaaaacg
     8041 aggtgttttt taaatttgat gactcaatat ccattttttg cattcttctt ttgaaaacaa
     8101 ctccattttt tctaataaaa taactggcaa tcccattctt ttaaatttca aaaaagttgt
     8161 atatgataca ttcaaatatt tagccatctc ttttcctttt agaaatggtc tttctaatcc
     8221 ggtatcaatt cttgcttgat caatagcctc ttttgtcatt tcaaaaacat aatttttcaa
     8281 ttgattcgtt tgttgtttat ctaacattac tgtaattcct gcatccatta tttttctcct
     8341 ctcattttct atttcttcaa tcagccaact cgccaagttg tatttattta gattttcaaa
     8401 tcattaagta accaatttac caacttttga taagtagatg ctctaatact ttctttgctc
     8461 ccattttcga ttttgacata tgttttccga tttataccta tcaattttgc tgcatcgttt
     8521 ttagtaagta taagatcggc tctatgtctt cttaagcttc taatgaattg atcattaaga
     8581 gttatcattc agcgcctcct ttcaaattat tcactttgtg gtttttatct taataagcac
     8641 attttaaacc acaaaacgag taatatcaat taaaatatta ctcaatttgt ggttttcatg
     8701 ctataatagt ttggaattta ctataggggg cgttactttg aatagaataa aattttatat
     8761 tgattccagt aaaaagacca ttaaacaaat tagtacagaa actggtatta actatacaac
     8821 tcttacgaac tattaccgtg gtgagcgaaa tcctagaaat actgaaacat ggaatatcct
     8881 tgctgattat ttccatgttc ctgtaagtta tttgatggga ttagacgaaa caaattccat
     8941 agaacaacct aatcttgata gaataaaaac tgctcgttta gaaaaaaagg acaaaatagt
     9001 caagaatatt gccagtcttg actcagatta ttttactgaa ttatctaatc tatacttttc
     9061 catgacttct ttacttgaag tcaaacttca agaagatgac tccgactttc tttttttaaa
     9121 aagtattcta gaggactcct ttgaatacac gattctattt gccaaacaaa agaagtttat
     9181 taataaagct ttaaacttaa atatcgatga aagaaaagag ttagaatctg agttatttgg
     9241 caacttaacc caagtacagg ttaacaatct aaaaagagag ataacagctc gattaatatc
     9301 aaaacttcaa taaaactaat tattcaatca gccaactcgc caagttatca gattggagag
     9361 attagttatg tcaatacaaa agtacacaac aaaaagcggt gcacgatata gtgtccgctt
     9421 gtatgttcgt aaagatgaaa atggaaagca agaatactat gtaaaaaaag ggtttaaaac
     9481 tgaaaaagaa gccaagttac acgaagcaag aaaaaaggtc gagattgaag ataccggcta
     9541 tcataagcct tctaatgact tgtttataac agtgtatgaa gaatggttgc cttcttatag
     9601 gaatcgtgta caagaaacga catatcaacg tacaaaagac cttttccgct tgcatatact
     9661 accaaaattc gggaataagt ccatttctaa gattactcct gttcactgtc agaatgcagt
     9721 caatgaatgg gctttaaaat tcaagaatat caaacagctt aagtcttaca gctctcaaat
     9781 atttgagtat gccatttttg cagagctatt acaccgaaat cctatggcaa atacaaatct
     9841 accaaagcgg gaaaagaaag aaaatgaaaa ttacttttca ttagaagaac taaaagcttt
     9901 ttgggatatt ctcaataaag aagagccttt aaaacatatt ttaatcttcc agcttttgat
     9961 tactactggt atacgtaaag gagagctagc tgctcttcat tggtctgaca tttattttga
    10021 agaacagcta atgtatgtta gaaagagcta tgcaactatt cgaaatgaag agaagaatgc
    10081 aagaagaaaa actgtcagga ttcaaaaaaa tacaaaaaat atatcttccg aacgaatatt
    10141 accaattgat gcacaaacta ttgaaatgtt aaaaatctgg aaaaaagaac aagctcgaga
    10201 gcttttgcag tttggcataa atacaaatac aaaaaatcaa cttatcttta cctatgtaaa
    10261 tgcagacggt gacattaatc aacctttgca tgcggactat tcaaataata tattgaaacg
    10321 tttagagaag aaatataaat ttaagcacgt tactattcat ggattccggc acactcacgc
    10381 tactcttcta ctagaaggtg gagcaagtat caaagaaaca caagatcgat tgggtcataa
    10441 aaatgctgaa actaccctta acacttattc tcatgttact gaaaaagctc aacgaaatgc
    10501 gttagaaaaa ttcattgctt acactggttt ataatattgt cattaccttt ctcattacca
    10561 gaacgaaaaa aagacacctt ctcgcaatga gaaagtgtct gaaatcgttg gtataacaac
    10621 gattaacgtt ttgaaaactg tgaagcttta cgtgctttct taagacctgg ttttttacgt
    10681 tcgaccatac gtgcgtcacg agtaagtaag ccagcacgtt taagagctaa acggaagtca
    10741 ggatcaactt ctagcaatgc acgagcgata ccatgacgga tagctcctga ttgtccagcg
    10801 tatcctccgc cgtttacgtt aacaaatacg tcatatgcgc cttttgtttc agtaacagcg
    10861 aaaggttgat tgattacttc acgcaagtca gcatgtggaa tgtattcttc aacatctttt
    10921 ttatttacag taattttgcc agttcctggt actagacgta cgcgagcaac tgcgttttta
    10981 cgacggcctg tgccgatata ttgaacttgt gccaatgaaa ttccctccta ttagattaag
    11041 tttgtgatgt ctaaaacttc tggttgttgt gctgcatgtg gatgttctgc tccaccgtat
    11101 acgttcaatt ttgtaaattg tttgcggcct aaagtgtttt taggaagcat accttttaca
    11161 gaagtttcga tcaaacgacg agaattttta gcacgcaatt cgccagcagt gattgatttc
    11221 aatcctcctg ggtagttgct gtgacggtaa taaattttgt cagtcgcttt tttacctgtt
    11281 aatttcactt tgtctgcatt gatgacgatg acgaaatcgc cagtatccaa atgaggtgtg
    11341 aaagttggtt tatttttacc acgtagtaca gatgcaacaa ctgttgaaag acgtcccaat
    11401 ggaacatcag ttgcgtctac tacataccat ttacgttcta cttcgcctgg tttggccata
    11461 tatgttgtac gcacgttatt ttcctccaat ttaagttctg atgtttgaca tacacttcga
    11521 gtttccgggg ctctttgtgg ggcaaacaat accatttaac atattacccg ctaatctagt
    11581 caatgtcaac gtttttctgc ttgattgaaa aatttatatg aaactttact tcttttcttt
    11641 cttttatata aaagggtcgt ttcctgtcaa aatgaaaaac aaaaagaggc tgtgacaaaa
    11701 gtcttgtcac aaccccttat tttgaataaa cggtgttctc ctgccaactc ctcggtcaca
    11761 agtcacaata tccataaaac ataagaagct gttttttaaa attgtttctt gtgactcgga
    11821 gctgaacggc aggcttacaa cctcttggta aacggtgttc aatcagctga ccctcggtat
    11881 tagctcaaaa aacggcaaaa tatgaggaac tattttgacg tttgtttctc taatactcag
    11941 gtcaaaacgc tgatttcaca acctcttttt cggtcaagtt caacaacagt ttttacgcgt
    12001 cgatctctgg cgctttgccc aaatgagctt ggatcatcca gatacgtttt tctgtggctg
    12061 ttttgaattc gataaagata tcttgtgttg gatcgtcgcc ttcttctcca gaaacttcga
    12121 ttcctttttg gtaaagatct gctagataac gatagcctgc aacaagtgtt tccaaacgtt
    12181 ctggaatagt acgatcccag cggccaattt catcagggat ttttgtgtta tctgcaaatt
    12241 cacgcaatgt agaataaggt gaaccgtcta atgtgatcaa gcgttcggat acttcatcta
    12301 actgagcgtt caaatcatcc atgaactcat ccatcattgg gtgcaatgta aggaaacctg
    12361 gtcctctcat gtaccaatgt gtttggtgaa caacaacaga catttggctt agatctgcaa
    12421 ctaattgatt taatacttct ttggtttgtt ggaatttcat ctttcttgcc tcctaacatt
    12481 ttcttacact taaaataata gtagactttc aatgaaaagt acaacaaaat catttacctg
    12541 agaacgattt tcagttcgct tttttatccg taaagccaat catttttcag aatcagctaa
    12601 ttcttctcgc agttgagcaa attgctgata agtctgtgct aaatgctttt gtgtcgaaac
    12661 aaataatgga tagattttct gatatttttt atgatcagtt tcttgtactc caacttgctc
    12721 tttcaattct atcaattcct gtgcttcact gatactttgg atctttccga tgcttctcca
    12781 gcctaaaatc acagctccta atgcggatgc ttctgtgaca gtagggaaaa caagcgggta
    12841 acccagtaca tcagccatca tctgacgaaa aatcggagat tgagcaaacc cgcctgtagc
    12901 acggatctct gtgagctgat tttcctggca aactcctttt aaaatccgat aaaggttgag
    12961 acagatccct tctagaatcg ccttcatcat ttctttttct gtatgttgcc gctgtaaacc
    13021 gataaaactg ccaaacgcat ctgcgtccca aagcggtgct cgctcaccaa gtaaataggg
    13081 atgaaaaatc agaccattgg cacccgctgg cacaaaagcg atagccgaaa gcagtgcatc
    13141 atatggattt tctccttttt ctttagcaga ttgaacgact tcctgtaaaa atgtatgccc
    13201 tgcccaatca aagatgccag caccattgct agtcgctcct cccactaccc aatgtttctg
    13261 atccagaaca taacaaaacg tctccatttc tggatggaac tgaggctgag tagtaataaa
    13321 acgaagtgct ccgctcgttc ctacggtcaa agcggcgatc gtatcaccaa aagcacctaa
    13381 ccctagatta gaaagcggcc cgtctgcccc tcccaaaaca aatgatatat catttggcaa
    13441 acctagttct gctgctgttt ttgatggtag acctgttact tgataggtgg aaggaaagac
    13501 ttgcggcaat tgctctattc gaatgttcaa gtagtcaagc acttcttctg cccattggaa
    13561 ttcatggata tcaaaatatc cagttcctga agctgttccg tagtcgctga tcagttcgcc
    13621 aaacaatcta taaaagatat agtctttgat accgacaaaa taagcagttt ttttgaataa
    13681 ttcaggttgg ctgttttgca gccacttgat tttgttcaat ggtgtcattg gatgaatcgg
    13741 cgtcccagtc tgccgataaa attctttgcc aagcacggta tatttcagtt tttctgcttc
    13801 gtccgctgct cggttatctg cccaagtgat cattcgagat aacggtgttt tctcttcatc
    13861 caacaatatc aggccatgca tcgcagagga gaaggagaca aattgaatga ttgtgtctga
    13921 aggcaactgt ttaattactt cttttataac ggtgcagaca ccttgaaaga tcaattcctg
    13981 atcttgttct gccatccctt gtgtttcttg gatcaaaggg tagcgaaaat aagcggatgc
    14041 tgcttcttgg gcttcatctg tgaaagcaac tgctttggtt tgtgtggttc cgatatctaa
    14101 tccgattgaa acgttcattt tttcacctca aattttatgt atgtacaaaa atttagttta
    14161 agcttgcgta tcttggtttt gaaaaggggg agatggcgtg ttactttatt ttatcattgg
    14221 ttgctgtatc ggatcatttt tatgtttaac cgcccagcgg attccgttag gtcactctat
    14281 tatttatcct agatctcatt gtgtcaaatg ctgtcgttcg ctttcttggt atgaattgat
    14341 tccgattctc tcgatcatcg tccaacgatt tcgctgccgc tattgcaggt gtcggttgcc
    14401 cttctactat ttgttggcag aagccttatg tggtggtctt tttgcctggt tctttacttt
    14461 ttcttccgca agaaattttt cgacgttcat atggatgctt tcggctttgc tattttcgtt
    14521 gatggattta ttttacttca tggtccattc tcacacgcta ttctgttctt gggcaatttt
    14581 atggattttt tggttgcagg ccggcgtctt ccaatggcaa agtgtgctgt taacgtttat
    14641 cgtaggcggt gtgtgccttc gctatggtca atcctatctc ggagctggcg atactctgtt
    14701 gatgctttct tggagtggtg gcttgtctct agaagagttg cttcagatct tgtttcttgc
    14761 tagtctgttt ggaatatccg ttttttctgt ttactttatc tgtcataaaa aaaagttgga
    14821 aaagctgcct ttcattcctt ttttgagcag tgcgttgctg attgtccttc atcttaggta
    14881 ttgaaaaaag gttgtgacaa acgacttgtc acaacctctg atatatagtg ttctcttacc
    14941 aaatccttgt gacttggagc taggcgacag gttcacaacc tctccactaa atggtgttca
    15001 atcagctgac cctcagtatt agctcaaaat gctggtttta caacctctga tatacggtgt
    15061 tccataagta gctttttcgg cttcttattt cttgacgccc caatgtcgac gaaggaaacc
    15121 tgcacgtcca gaaccttctc gataaagatt gtaatggagc ttgtttttct tgtaataatc
    15181 ctgatggtaa tcttctgcag gataaaattc gcttcttggt tcgatttttg tcacgattgg
    15241 cttatcgaat cgcccgctgt cttgcaatgc ttgttttgaa cgctctgcaa tatttcgttc
    15301 ttcttcatca ttatagtaga tcactggacg ataagaatca ccgcgatcag cgaactgtcc
    15361 taatgcgtca gtcggatcgg tttgctgcca atagatttcc accaactgat cataagaaat
    15421 aacttctgga tcatattcga tctctactgc ttctgtatgc cctgttgtgc ctgttgttac
    15481 ttgttcataa gttggatttg gtacatgccc gccagtataa ccggatgtca cagaaatgat
    15541 ccctggctgc tcttcaaatg gtttgaccat acaccaaaag catccgcctg cgaaaactgc
    15601 tttttttgtc atataaaatc cccctttttt ctcatcatac cttgatttcc aaaaggagga
    15661 taacgttttg cttgtgacag aaaaaacgga aactattata tacgtgatac ggcttctgat
    15721 aaatgccaaa acgaacgtac ttgatcaaaa acggttaaat caggaatcac atattcagca
    15781 gaaggaagat ttatgttttc acctattccc aatacatgca tgttaccagc caaagctgct
    15841 tgacaaccag cttctgaatc ttcaatgacc aagcatgcat ttggcggtac attcaattgc
    15901 tgtgctcctt tgagaaaaac ttctggatca ggttttgctt ttgatacttg cgtgccgtca
    15961 ataaggacat caaaataact tgtcaaattc gttttttcta aaatgaggcg tgcatttttg
    16021 cttgctgatc ccaatccgat cttgatgttt gtttttttca agtactcaag aacagctatt
    16081 gcaccaggca aaacggatgt ttcatccatt ttttgaatcg cttgaagata tagattgttt
    16141 ttttgctcag caaaggctga tttctctttt tctgagaatt tctgttccat ttttccaaat
    16201 gacagcaatc gctctagtga ctcggttctg gatattcctt tcagttgctc gttttctttc
    16261 tcagtgaatg gaatggacag ctggttagct aaaaccagcc acgcttcgta atgataacga
    16321 gctgtgtcta caagaacgcc atctaaatcg aatagtgctg ctttgatttt catcgattac
    16381 ctccttgaaa atgaacgact tgaagtaaag atgcgatgtt tttagctgtg cgatgaagat
    16441 tatcagtggc ttgctgtaaa gcttcttgta aaggcacagc tgcaggaagg atcggaaaaa
    16501 cggccgaaat gccaacatct gctgcttttt cgcttccttt accaacactt ccgcaaatag
    16561 ctatcacagg aacgccctct ggtactcttc tagcaatccc aactggcgct tttcctttta
    16621 gactttgtcc atctagacgt ccttcgccta cgattacgag gtctgcttgc tgtacccgtt
    16681 tatcgaactc cagtaaatcc aatacgtagt cgatgccaga gactattttc cctgaagcaa
    16741 atgctaacag tcctgcaccc attccaccac cagcactact gcccgatgcc tcgattactt
    16801 gaggggcaaa tctcatataa aaatcatgca tggcttggtc agccactgct aattcatttt
    16861 ctttcaagcc tttttgtggt ccgaaaacaa aagaagcgcc ttcttttccg cataaaagat
    16921 tagtaacatc agtaatgaca gtgattttgg ctttaatcag ctcaataggc acgttcgttt
    16981 tatcaactgt agcaatttta cctagatttt ctcccagtgc ttctactttt tgattatctt
    17041 gatcgtaaaa agcataacct agaccttctg ccatgccgat gccgccatcg ttggtactac
    17101 ttccgccaac gccgatgatg atgtgtcgaa caccttgttg caacaattgt cgtatcagtt
    17161 ctcctactcc tcgcgtggat aacctaagtg gctgttgttg gtttagtggg acatctttca
    17221 atccgacgat ttctgccatt tcaaaaatcg cagtttgacc gtctgaagcg tactggattt
    17281 ctttgttttt gccaaatgca ttagtaacga ctgctgtttg ccgcttcaac cttaaactgt
    17341 ctgctaatga tgaaaccgtt ccttctcctc catcgcctat aggtagcaga tcatattcag
    17401 cctgaacaaa aacttcttga aaacctgatt gaatggcttg tgctgcctct aaggctgtca
    17461 gactttcttt gaaagaatct ggcgcaatca gaatacgcat tattttcccc tccgttccaa
    17521 gatcagttct ttttccagct ggtattggtc tccattgaca aaaataggta atgtttcttt
    17581 cgtctctttt tttattacta tttgtttttt tgtcaaaaca aatagcaacc tgtgattttt
    17641 ccaaataaaa ggaaaagaaa ttttttccca tgctgaagga agtcttggag aaatagaaag
    17701 agttccattc gttactgtaa tacctgaaaa accaaatata actgctagcc atagtgctcc
    17761 taatgaagca gcatgaattc cgtcatctga tgaatgcgga tctggtccta aatcaatcag
    17821 gcacgcttct tggaataaat gatacgccca ttccatttct tggatacggc aagcctctat
    17881 ggcgtgaatc gctttactca acgatgagtc gtggattgtt ttgttttcat agtaatgcaa
    17941 attcttagaa acgattggtt gagaaaaata ctcaggtaat aagtataaca gcatgacaag
    18001 atctgcttgc ttgctgactt ggagttcatt tacttcgcta cgggcataat caagtaaaat
    18061 agactggctg ccaggacgtt ctttgtaagt ggtcaaatca ataactggct tactgagata
    18121 cgtgtcatct tgcggaatca gctgttcttt gttaggcact ggcaaatata acttatctaa
    18181 aaaattttga cattctttca aaaaaagcgg atcgccagct tgatatttaa cagctaaggc
    18241 cacattataa tgcgccagat agttcgtata tgcattattg tcgatatatt ctgtgtattc
    18301 atctggacca atgactctgt gtatctctaa atgatcatcg acttttacag ctctgctcag
    18361 ccaaaaagct gctgtttcat gaatgagcgt ttttcccttt gtttctaaaa acgattgatc
    18421 ctctgttgct tggacatact cttgaacggc ataagcaagg tctgcactga tatgatgttc
    18481 tgcggcagct gaagcaactt cttgtcttgt tccggttttg atattcaatg cagcaaattt
    18541 gggtgtctct tcctgaccag tgtgtgcgct ttcccatggg aaaagtgcac cttgatagtg
    18601 ataatctctt gctttttgcc gtgcgccttc taatcgatca taacggtaca gcagcaaatt
    18661 ctttgctact tcaggaaact gatataaaaa gaaaggcagg ataaaaattt ccgtatccca
    18721 gaaaacatgc cctttataac cttcgccagt tagtcccttc gcccctacac taacacttgg
    18781 atcattgcgg ggagtcataa tctgtaaatg gtaagcagcg aagtccatcg ctaactgatc
    18841 gaaagaagaa tcgccttgga tcgttatccg atgctgatgc caaaaatctg tccaataaga
    18901 catcgactgc tctaaaattt ctgtataact aacgcttttc tgggcttggg caatactttt
    18961 cttaaacggg tctgtttgct gaagagaggt agagagcacg atgatcttct ccagcgtaaa
    19021 ggacgtattt tttctgatct tttctgaaaa ggaagcggtg atccggcgat tttttgcttg
    19081 gaatacacct gctttattga atttagctgc aatcccaata tcaattttac tttctgttgt
    19141 ctggtaggat gcactgatca aatcgtcacc aaatactcgc agttcttttt caaccaactg
    19201 ctgtgtacca aaattggttt gctgtccatc gatacccgtt ttcacatcta cttggcagtc
    19261 ttcggtcaac ggcttgatga ccactttcag ggctgcctga tggagattat tttgatcagc
    19321 aacacgacaa gtaatgactt ccactttttt tccttgagca gtttcaatga gcgcatagcg
    19381 tttaagctct cccatttcta aatctagtgc ccgctcgtat gcaagaactt tcatcgtctc
    19441 aagagaaaaa tgttcaccgt cgatttttat ttcccatctg atgacatctg ggagattcac
    19501 taactcggct gattctgctc ctaaaggtcg gttatataca ccagctgcaa acattcccct
    19561 tacttgattc cgatagtctt cctcctggct tcctctgata ccaaaaaatc catttccaat
    19621 ggtcatctga ctcgcatact tattgatata ctctaaatca aactgttttt cttctacttt
    19681 cataaggtaa taacttctcc tgtttctgcc gaacgatata aattttccac cagattctgg
    19741 atgacatatc cttgccagcc atctgcaatc atctgagtct ggtcaccgag cgcccgattc
    19801 acaaaatgag ccatacttag ttcatgtggg tcattaactg gcgtggtctt ttgtaaggaa
    19861 accagctttc catctacatc ttcgaatagt tcgcctggat acagattagc acctgcctga
    19921 tcgccacaaa aagccacatt cagtttgtct tctctgatat tcaaagcaaa cgaagtattc
    19981 agttggatga tcttgccgtt ttctaattcc agtgtgccaa aaattgcatc ttctactgtg
    20041 aatttgcttg gatcccattt cccgaatttc cctgaatttt ttttcgtacc aattttttgg
    20101 aagctgtacg cccgaacgct ttttacttta ggaaatccag taatatacat cgctgtatca
    20161 agcatgtgaa tgccataatc gatcaaaggc ccgcctcctt gaagttcttt gtcgataaac
    20221 gttccccagc ctggaacacc agaccgacga agcgcattcg cttctacgta ataaatatcc
    20281 ccgagaacat caagatgatt cttgatcatt gttgcttcgt cagaaaaacg atgctggaat
    20341 ccataagcca aaatctgccc gtttttatcg gctgtctctg ccatctttct tgcttgatca
    20401 gctgtcatag ctggtggttt ctcgcacatc actgaagcat tagcttcgag ggcagccaat
    20461 acatgttcga agtgaaagcg attgggtgta cagacactca cgatatcggg agaaatctca
    20521 ttcagcattt gtgtaactga tgtatacgca tgagaaaatc ctgtacgtcg gcaaaagtct
    20581 tctgctcgct ggctgactgg atcaaccaca gcggccaatt ctaagacatc tgatcttttt
    20641 tgataataag cagcatgtac tttttctgct acttgtccag cgcctatgat tgctactttc
    20701 ttcatttatt tctctctcct ttgcttattt acttattgtt attctttctc tattctttct
    20761 ctaaatcatt taaatgacgc gctgtttcca gacagttttt caagtattgc aatgattcct
    20821 gataaatttt cacttcattt tctcctcgca aacgacattc atataccata taaccttcat
    20881 actcattttg aagtagcgtt tgaaaatggc gagtaaaatc aattgcgcca gatcctggtt
    20941 ggtagcgatg attatctgct aaatggatat gacccacata ctctttttgc gtagtcaatg
    21001 cttttgaaat atcatcttct tcaatattca tatgataaaa atcagcgatg atctgcacat
    21061 gtttcaattg attagagtca atgtaatgac gcgcatccgc taacaaattg atcatatggt
    21121 cttggtatcg gttcaaaggc tccaaataaa taaccgtacc tgtttcttcg gctactttat
    21181 ctaaataacg aagtgattcg ctgactgctt tatagtctcc ttctttgctt cggggtgaaa
    21241 ccatcggcgg taatcgataa gtgaacattc cccaagctgc tgggataacg attcctttgc
    21301 ctccaacctc gcttagtgca cgcaaaatct cagcaatttg acgtacacca ttaagacgcc
    21361 gttcttcgat aaagtcaccg atccacccat cgtagccgtt acaagctgtc actactggga
    21421 tccctgtttc tttgattgcc tgtttgactg tgtcgatatt ttctattaac aacttaccgt
    21481 cgatttcgta ttcttcaaat ccacagtctt tgataaatct aaatttttct aaaatgtctc
    21541 ttgggaaaaa tgcttgatct tgcgtaccga atttcattgt tctcctcctc ggacttgaac
    21601 gcccattttg atactttctt ctgggtgctg gtctacataa cgcatgtaag cttctgcgct
    21661 ttctgcaaat ggcacaactg gatcaatgat ttcttcacaa tccaagtaac cgttcatcaa
    21721 taattcccaa catgtgtcct cgattcgttt acggttccat cttgggtaat ctgggtttgg
    21781 ttcactagat gctcgagcaa agacgatttt tgcattattg aaatgtgctt cgcgaccaag
    21841 attaaaccca gctggaaaag gtttagcaaa ggcaacataa gaaatgatcc cgccatagcc
    21901 gatccctatt aaagaagctt gtaatgcggc agcactgcca ctcgtttcaa taactgaatc
    21961 tactccacgt ttatctgtca attcctttag acgaaaaccg acatctgctt tcataggatc
    22021 tagcgtgtaa tcggcaccgt gtttttctgc taattctcta cgctgcgcta tgggatcgat
    22081 cccaacgatg gtagtagcac ctgctttttt agctaattga atcgcaattt gcccgatagc
    22141 gcctaaaccg aagattgcta cagcatcacc tgctcgaaca tgtgcttccc ggataccacc
    22201 taaagcgaat tgagcaggat cgtaacatac tgcatttttc cattgatcca accgatcaag
    22261 tttacgtagt ttataattat caacaccgtt tgtaatcagc gtttcacgaa tgggtccata
    22321 agtgcagact aactctcctt tttgatactc tgtcaccaaa ctgcccgttt cttcgatcgt
    22381 accgacaatc atatttccta attgaaattc gccaaatacc acaccgcgtt ggcttccttc
    22441 ttctcttggc aagaataggc gccattcttc tgaaaattct tcatcgataa atggacttaa
    22501 tcctctaaag tcgacaattt ctgttcctgg ttttggagta gcgaaaatta ctcggatacg
    22561 tacttcgttc ttttcgatct cacggtcttc gtaatctttg aatccggcta ttcttggttc
    22621 taatgcaact aattttttca tcttttcttt cctctttctt ttctttttat ccttttacgc
    22681 cgccttcgac taaatcacct ttaataaatc gttcagataa cgcatacata atgatgactg
    22741 gtaaagcggt caccaatgaa gcggccatca ttcgtcccca aatgtagtct ggtgtactga
    22801 ataatgtgtt caaaccgatt ggtaaagtaa atttttcgga ggtcgacagg aaaatcgaag
    22861 caaataaata atcattccac gaaaccataa agcaataaac gaagacagaa atgattccag
    22921 aaatagccaa tggtataatg atccgaataa tgatctggat ccggctaaga ccatcgatca
    22981 ttgctgcttc ttcaatatca gtcggaatcg tgtcaaaata actttttagc atgaatatcg
    23041 cagtaggaag tgtttgaacg accatcgtga taatcagtgc tgtattggta tcatacaaac
    23101 ctagaccgga aatgatttta aataatggaa caacgatcaa aataccggaa aacatataaa
    23161 cggtgtaaaa gctagcattg attttcgagc gagctttgaa gcgtagtttt gacaaagcgt
    23221 atgccccgct gatcccaatc actactgcag ttacggaggc tatcaaggag atcactaaac
    23281 tatttttgaa atacatgatg aatggaaaaa tctcagcatt gaaaatatcc tgataatgtt
    23341 caaaagtcca ttctttcgga aaaatagttt gtgtcgttgc aattgcttct tgtgaatcct
    23401 tgaatgatgt catcagcatg atcaaaaatg gaaacaaatt gacagctaat agcaacaaaa
    23461 tcgtcgcgta aaataagatt ttcctgctct ttttattttt catttcaagt ccaccttctt
    23521 tcgtgagagc aagacgaccg ctgcaataat gatgaacaat agaatcgata tagctgctgc
    23581 ttttccaaga tcattgaacg caaaggctgt ctgatacaaa taaacgccta agatattgac
    23641 tttattcgtc agtagataga catctgtaaa catatagaac atccagatag atcgtaaaac
    23701 aatcacagtt gctagtactg gtttgattgc cggcagtgtc acgatcttga acttctgcca
    23761 agcatttgca ccgtccattt cagctgcttc ataaagcgtt ttgtcgactg tttgcaaaat
    23821 agccaaaaat gaaataaacg catacggata gtatctccag atagcaaaca aaacgaccat
    23881 aaagaagcta gcaactggct ggtcgaacca cagcggtgct tcgttgaaaa gctgcaacct
    23941 atctactaac agatcattga cgattccata actattattg aacatatatt tccaagcaaa
    24001 aatcaaagaa atagatggcg taacataact taataaaatc agtgaacgca ccgttttcct
    24061 aaatctgaac ttgcggttaa gcaaaatagc tacggctaat cctaaagctg tactgcctgc
    24121 tacaactaag aatgtgtaag caactgttaa acctaaagaa cgataaaatt cactatcact
    24181 taaaatttca agataattgc ttaatccgat aaaaacggcg tctgagttag gattcagcgg
    24241 catatcgaaa aaactgatct gaatattgga aagcattgga atcagcacta aaacagctaa
    24301 taaaagcatg cttggtgcta aaagtcccat ggctaatttt aagtcagaat gtttccttgg
    24361 acgattcatt gaaacctccc cctgataatc tgccaatagc tatctacttt tttcgaacac
    24421 cattggcaga acttcttgtt atttcgtatt tttttgtgcg tcagctaaag cttgatcgac
    24481 atcttcgttt ccgacagtta cttggttcac tgcttttccg atcacacctg agcttgtgat
    24541 atctcccatt ttcgtgaaat tcttttcccc gactagtcca aagacttgta cttcatcaaa
    24601 agcactagca atctcagatg gcagctcgcc aaaagacttg atgacttcat tttctttgta
    24661 accatccagt tcgactactt gtttattcac tggttgagca ccaccaggag acatcaagac
    24721 ccacttttcc atgtttttcg gttgagataa gaaagctgtg aatgcttcag cagcctcttt
    24781 ttgtttctcg tctagaccag aagaaatcgt caatccagat actgtgccat agacagcttg
    24841 atctttttgt gttgggatag caaaaccgat attttctgag ttccccgctt cataaacaga
    24901 aggcaaaatg taagttgaat agattgccat tggtgctgtt ccattcatga aagcatcttt
    24961 gatttctgtc acatcattag agcctggcat cgtgtattgg gataagtttt gataaaacga
    25021 caatgcttct ctcatctttt ccgtatcgat cgttacctcg ccttcatcat ccaaaacatt
    25081 tgctccattt gacaaagcaa attgagagaa cgcttgctca ctcattgttc cttcagcagt
    25141 aggcatagca attccatatt gtttattctc tttatctgtg aaatgttgag caatttttaa
    25201 aatatcatcc caattttctg gttctgaaaa accagcttct gataattttt ctttgtcata
    25261 ccaaatacct tgaacccagc cgctaattgg tgcagcaata tagctttttc catcttctga
    25321 acgaacaagg tttttggctc cgtcataata attatcttct ccagcttcct cgataacgtt
    25381 ttggactgcc gtctgatcaa tcagttcatc tttatccatc actttagcaa aatcctgact
    25441 gacttccatt acttctggta atttaccgga acgagctaac gtaacgattt ttgtattgaa
    25501 cgctgattct tctaccggca cttgcttgat tttgatgtct ggattttctt tttcaaaatc
    25561 agcaaccagt tcattgatca cacttaatcg ttcctgttcg acagaagagt gcatgaattg
    25621 aatcgttgtc tgttgtgatt cgttagagct ttgactttca ccacaagcag taagaagaaa
    25681 tccagtagct agcagacagg tagtaacaat aatttttttc atttttttcc acctcttatt
    25741 ttattatttt cagccacata tattcatacg gctggaatat caactttcgt tcttgactgc
    25801 aatggataat ttgtccagaa aacaggtcta ggtaatctcc ttttggtaaa agataacctg
    25861 ctgattgatt ggaaaaatta tggataacta agactttctc ttccttcgct tcgcgaataa
    25921 aactgataaa agaatctgct gagtccagta agtaaatcaa actattcgga tggaaggctt
    25981 tttctttttt tcgaatagat agcatctggg tcaattcacc atatactgct gttcttaatg
    26041 gatctgtctg caactctttc tcaatatctg ctaacaaaaa tttttgccga ttgatttttc
    26101 gattctctcc tgttttttct acgcctttgt aatcatttcg acttcccagc acactttgaa
    26161 tataaattgc cggcacgcct ggtaacgtta ccaaaaatga atgagcagct aaaaagcgtt
    26221 tgcttctcca ttccgtcgta tctgttggag aacttaacgc atctagatac gtagcgttta
    26281 tctcatatgg actggtcgtt ccatctgggt ttttcttgta gtttactcgg gcaccctctt
    26341 tcttcagctg ttccactaat tcaaggatcg ttttttccgg cagaatacct cgtaccggat
    26401 tcaatccgat accgtcatgg gaggctaaaa aattaaaaaa agttgttttt ttcgatgcga
    26461 aatcgatggt ttttaaccat tctttcaact tacttgtatt acccgaacga atcgcttgaa
    26521 gggtcaaagg tggtagcgaa aactgataga ccatctgtgc ttcattctcg ccattcccaa
    26581 aataactgat attatctttg tgtggcacat ttgtctcagt gatcaatacc gttcctggag
    26641 ctgcttcatc agcgattgca cggaataatt ggatgatttt atgcgtttct ggcaggtgta
    26701 tacaagaagt tcctagttct ttccataaaa atcccacagc gtccaagcga ataaatgaag
    26761 cgcctttttc taagtaaaac aaaagaatat cgatcatttt caataaaact tctggatttt
    26821 taaaattcaa gtctacttga tcttcgctaa aagtcgtcca tacgtgtttt tctttcccgt
    26881 ttttgtcaat gaacgttgta agtaacgggc tggtccttgg tctagtcacg ctgcttgtat
    26941 ctgtatccag tggcaaatca ataaaaaaat ctttgtacgt atcattcaag tttaaatatt
    27001 cctgaaacca agtactttcg gcagacatat gattgcagac aaagtcgaac attaaccgtg
    27061 tttcgcttct catttcttct atatcttccc atgtgcccaa acggtcgttg attgcctgat
    27121 aatttacaac ggagaaacca tcatcagatg tatatgggaa aaatggcaag aaatgtacaa
    27181 ctctaaatgt atctgccaaa tacttttgat aaaatttctt aaatgtacgc aaagttggtt
    27241 gctgctcttc aaaaaattga tccccataag taatcaagaa aatatcttct tcgtcccaat
    27301 gcagtttgcc tgcctggcct ttcttctgct gataaacact gactttttgt tcaatcatag
    27361 aggcggtttt ctcagacaaa tctgagtcgc gataaatcgc ttgtaaaagt tttgtataca
    27421 tcggattccc ctttcttttt tgggagcgtt cccaacgatt tgatcatatt atggaaaacg
    27481 cttactgtca atagcatttt tttaaataat tttgatatac acaaaaatac aaataagaaa
    27541 gcttatgtat caataattaa tagcttctta aaaaaatatg gtctctcatg atcatgcaag
    27601 ctattcctaa attttcaaaa tcatagacaa aaaagaatgt ctattgtact atggaattat
    27661 tctaaaacgc tcccaaaaag gaggtgcata taaaattgaa acctactatt tatgacattg
    27721 cgaaaaaagc taatgtctca aaatcaactg tctccagagt attaaataac cagccaaata
    27781 tctctgataa agcaaaagca agtgtacttc aagcaatcaa agaattgaat tatgaaccga
    27841 gcaaaattgc aagaggactg tcaggaaatg gttttgacgc gattctagca ttatctcatc
    27901 gtacttctca tacgacctat ggcaatcctt ttttttcaga tgttattcaa tccatttctg
    27961 ccgtttctga acaaaataac ttcgacttga ttctacaaac ggcaaaaaca ccagaagaag
    28021 agctagaaaa atgcgcgaaa aagatccaag aaaaaatgat ccgaggaatt attattttaa
    28081 gctctacgat ggatgaaaca cttctttttg aattggatca atacaatatt cctattgtgg
    28141 taatcggaaa agtcggaaac gactacacga acatctactc agtggacacg gataatttcc
    28201 aggatagtta cgatttggtg gaaacactga tcaagaatgg acaccgtcat atcggctgtc
    28261 tccatgctcc gacagaagtt tatgtatcca atgatcgtct caatggttac cgtcaatgct
    28321 tatttgatca ccacctagat atccgtaatg aatggattat agacggcggc tttcgcttag
    28381 aagatagcat ggcagcaatg aatgatttaa tgaagctacg agaaagacca actgcgattt
    28441 tttctaccga tgcattaaaa tcattgagta tttacaaagt ggcagaacaa tctggaaagc
    28501 agatacctga tgatctttcg atcgcaggat tcagtgatcc cttattgtcc tccctatttt
    28561 cgccacagtt gactcggata gacattccga caaatcagtt aggaatcaaa gccacagaac
    28621 ttttattcca actaatccac caccaatacc ctgaagaaaa aagaatcatt ctttcaaccg
    28681 agattactct cacagattcg atccaacaac aagaaatgta gaacagaatt ataaaagaac
    28741 agaaacactg aggataagtc agttactctg ttcttttttc aatcactcat ccctcgttac
    28801 cccctcccgg tattttagcg aatttttctt attttccatc tgttttagat agctgtggat
    28861 aactataatg tttgtgttaa actatttttt ccacagggaa gaaaaaacta tttttaacca
    28921 cgttgtgtat aagtttttat agttcgtttt tttaaacaca tttaccctta tttcaatcca
    28981 tgttatttgg ggatacttat ctctaaaaca taaatagaat aacagacatg tctactggtt
    29041 ttgttttatt cgaggaatta ttcaataata gctagcaaaa tattaaaaat aaccgctcta
    29101 tcttcccatc aatttatttt gcattcaaaa aatgatctat ctttactttt gttgattgac
    29161 ttctcttgga agatgatcgt tccgatacct taattggata cgaaccgatg ttgttaagat
    29221 cctttcccac ttttcttcta gttagacaaa ttattttaga caaaccaaaa aagaggttgt
    29281 gacaataatt tgtcacaacc tcttttttaa aatatctatt ttgtttgatt gttcattgcg
    29341 tcttctgcag ccatttgcgc ttcaatatct gaaatactgt agacattttc gcttgctaca
    29401 ccgacttctt ttggttccat gtcacgataa cgttgcatac cagtaccagc tgggatgatc
    29461 ttacctataa taacattttc tttcaaacca agaagaggat ctttcttacc gcggatcgca
    29521 gcatccgtca agacacgagt tgtttcttgg aatgatgcag cagataagaa actatttgtt
    29581 tctaatgatg cttttgtgat acctagtaag acaggtcgag aagttgcagg aacgcctcct
    29641 gagactagcg tattgtagtt ttgatcttta aagtctgcga tgtctaataa tgtacctggt
    29701 aagatatctg tatcacctgg atccattaca cgaactttac gcagcatttg acgaaccatt
    29761 acttcgatat gcttgtcgcc gatttctacc ccttgcatac ggtaaacacg ttgtacttca
    29821 cgaagcaagt agttttcaac agataataca tcgcgaactt gcaacaattg ttttggatcg
    29881 attgatcctt ctgtcaatgg tgcaccacgg tggatcatat cgccttctgc tactttcata
    29941 cgtgctgtat aaggaacagt atacgtacgt gtatctgttt ttcctttgat tgttacttct
    30001 ttggtacgtg ttgcaggatc ttctgagata tcgatgacat cccctgttac ttctgtgatc
    30061 actgcttgcc ctttcggatt acgtgcttca aagatttctt ggacacgagg aagaccttga
    30121 gtgatatcgt ctccggcaac cccacctgta tggaatgtac gcatcgtcaa ctgagtacca
    30181 ggttcaccga ttgattgagc ggcgatcgta ccaactgctt ctccgacttc aacgtcagag
    30241 ccagttgcaa ggttgcgtcc gtaacaatgt ttgcagacac catgttttgt attacatgtg
    30301 aagacagaac ggattgtcac tgtttcgaca cctgcatcaa cgatttgttt tgcgatgtct
    30361 tcggaaatca attggtttcc tgcaatgatc aattcatttg tttcaggatg acgtacttct
    30421 ttacgggtgt aacgtcccac taaacgatct tctaatgatt cgatgatttc atttccctcg
    30481 cggatggctt ggatttcaag acctcggtca gtaccgcaat cttcttcacg gatgattaca
    30541 tcttgggcca cgtcaaccag acgacgagtc aagtaacctg aatcggcagt cttcaaggct
    30601 gtatcggtca tccctttacg agccccgtga gttgagataa acatttccaa gacggatagt
    30661 ccttcacgga agttcgaaat gattggcaat tccatgattc gaccattagg agcggccatc
    30721 aaaccacgca ttccggcaag ctgtgtaaag ttggaaatgt taccacgagc tccagaatca
    30781 gacatcatga agattggatt tttcgcttcc aaaccttcca tcagtttttg ttggatgctg
    30841 tcttttgcag cattccaaac agcaatgaca cgttcataac gttcgtcatc tgtgatcaga
    30901 ccacgacgga attgttttgt aatagtttca acttgtttat gggcattttc aatgatttct
    30961 tgtttttcat tcagtacact gatgtcagcg atccctactg tgataccggc atgtgtcgaa
    31021 tgtttgtacc ctaagtcttt catacggtca agcatcttag aagtttctgt tactttgaat
    31081 cgtttgaaga cttctgcgat gatgtttcct aagtttttct tcttgaacgg taatactaat
    31141 tcttgctctt tgatatgtgc aggaatatca gtaccagctt caacaaagta tttgtccggt
    31201 gtttggacgg tcaagttgaa gtctgttggt tcattcagat aagggaattc aggaggcatg
    31261 atctcgttga agatgatctt accaactgtt gtgatcatcg tacgttcttt ttgccattca
    31321 gtaaatggtt tttcacctaa tgagttaggg ttcacaccga tacgtgaatg taaatgaaca
    31381 tatccgttgc gccatgcgat aacggcttcg ttttgatctc tgaagaccat tccttcgcct
    31441 tcgcgtcctt cttcttccat tgtcaagtag tagttaccta gaaccatatc ttgagaagga
    31501 gtaacaactg gtttaccatc tttagggttc aagatgtttt gagcagctaa catcaacata
    31561 cgtgcttctg cttgtgcttc ttcattcaat ggaacgtgga cagccatttg gtctccatcg
    31621 aaatcggcat tataagcttc acatacaagt ggatgcaaac ggattgcgcg tccttgtact
    31681 aaaactggtt cgaatgcttg gataccaagt cggtgaagcg taggtgcccg gttcagcaat
    31741 actgggtgtt ccttgattac gtcttctaaa acatcccata cttcgtcttc ttgacgttcg
    31801 attttacgtt tcgcattttt gatgtttgaa gctagttcgc gttgaacaag ctcacgcatc
    31861 acgaatggtt tgaataattc aatggccatt tcttttggaa gtccacattg gtacatcttc
    31921 aagaaaggtc caacaacgat aacggaacga cctgagtagt cgacacgttt acctagcaag
    31981 ttttgacgga aacgaccttg tttccctttc aacatatgag aaagagattt caatgggcgg
    32041 ttacctggtc cagtaactgg acgtccgcga cgaccgttat cgatcaatgc atctactgct
    32101 tcttgcagca tacgtttttc attttgaacg atgattccag gtgcattcaa gtctaacaaa
    32161 cgtttcaaac ggttattacg gttgattaca cgacggtaaa gatcattcaa gtcacttgtt
    32221 gcaaaacgtc caccttctaa ttgaaccatt gggcgtaaat ctggtggaat aacagggata
    32281 acgtccataa ccatccagct tggttgattt cctgaagcgc ggaatgcttc taaaatatcc
    32341 aaacggcgga ttgcacgtgt acgtttttgt ccttgtgcag atttcaattc ttctttcaat
    32401 tcggctgctt ctgcatctag atcaacatta tcaagtaatt gtttaatcgc ttctgcaccc
    32461 atagcagctt ggaattcttg tccatattgt tcacgttttt cgcgatattc gcgttctgtc
    32521 aacaattgtt ttttctctaa attcgtattt cctggttcga tgactacata tgaagcaaaa
    32581 taaatgattt cttccaaagc gcgtgggctc atgtctaata caagacccat acgtgaagga
    32641 atccctttaa agtaccagat atgtgaaaca ggagctgcta attcaatatg tcccatgcgt
    32701 tcacggcgaa ctttcgaacg agtaacttcc actccgcatc gatcacaaac gattccttta
    32761 taacggatac gtttgtattt tccgcaggca cattcccagt cttttgtagg accaaaaatc
    32821 cgctcacaga acaacccttc gcgttcaggt tttaaggtac gatagttaat cgtctcaggt
    32881 ttttttactt caccgtaaga ccagcttctg attttttcgg gagaagccaa acctatttgc
    32941 atactttcga atttatttac atcgatcaaa aggggttccc tccatttcat ttaatggcaa
    33001 accgcaagac ggctgatctt cgaccgcctt gcagctcata gaccggatta gtctaaatta
    33061 tcttctgcgg tttcaaagtt ttggatgaca tcgtcttttt catcttccac ttgttcagca
    33121 gctttctttt ctaattcttt ggcagtttgc tgttcggcaa attttgtcaa agcatcaaca
    33181 gtgatcaaat cgtcgtcttc atcgtccatg tcgcgaagtt cgatctctgt ttcttcgata
    33241 tccaagacac gcatgtccag acctagtgat tgcaattctt tcaccaatac gcggaatgat
    33301 tccggtacac ctggttttgg aattggttca cccttgacga ttgcttcgta tgttttcaca
    33361 cgtccgacta cgtcatctga tttgtatgtc aagatttctt gtaaagtata agccgcaccg
    33421 taagcttcca gtgcccatac ttccatttca ccaaaacgtt gtccaccaaa ttgagcttta
    33481 cctcccaatg gttgttgtgt aaccaatgag taaggtccga ttgaacgtgc atgcaatttg
    33541 tcatcgacca tgtgagcaag tttgatcatg tacatcacac cgacagacac acgtccatca
    33601 aatggttcac ctgtacgtcc gtcgtacaga acagttttcg catcgctggc cataccggct
    33661 tcacgaactg tttcccatac gtcttcatca cttgcaccat caaatactgg cgttgctacg
    33721 tggataccta actgacgtgc agccataccc aagtgtaatt ccaatacttg cccgatgttc
    33781 atacgtgaag gcacccctaa aggattcagc atgatatcga ttggtgttcc atcaggtagg
    33841 aatggcatat cttcttccgg cataatacgg gatacaaccc ctttatttcc gtgacgaccg
    33901 gccattttat ccccttcatg gattttacgt ttttgaacga tataaacacg aactaacatg
    33961 tttacgcctg gtgacaattc atctccagct tcacgagtaa agattttcac atcgtgaacg
    34021 ataccgccgc cgccatgtgg tacacgtaat gatgtatcgc ggacttcacg agctttttcg
    34081 ccaaagatcg catgcaatag acgttcttct gcagataatt ctgttacccc ttttggcgtg
    34141 actttcccta caagcaaatc gccatcttgg acttctgcac caatacggat gatacccatt
    34201 tcgtctaaat cttttaatgc gtcttccccg acattaggga tttcgcgtgt gatttcttca
    34261 ggtcctaatt ttgtatcgcg ggcttctgat tcatattctt caatatggat agaagtgtat
    34321 acgtcatctt ttaccaaacg acggctcatg atgatcgcat cttcatagtt gtacccttcc
    34381 caagtcatga aaccaactaa aacgttttgg cctagagcca tttcgccttg ttccatagat
    34441 ggtccgtctg ccagagtatc ccctttttcg actttctcac ccaaatgaac gattggacgt
    34501 tggttgtagc ttgttccgga gtttgaacga cggaattttg ttacatcgta tttatctaat
    34561 gcaccgttgt cgcggcgtac gcggatttca gaagcatcaa cgtattcaac gacaccgtca
    34621 tgtttacata acaaagcagc tcctgagtca tgcgctgatt tgtattccat accagtacca
    34681 acccatggtg cttgaggatt gatcaacggc actgcttgac gctgcatgtt ggcacccatc
    34741 aaggcacggt tggagtcatc gttttccaag aaaggaatac atgctgtcgc aacggcaact
    34801 acttgtttag gtgatacgtc catgtagtct actttatcaa tagatacttc taagttttca
    34861 ctttgagcac gagccataac aacgtcttga gcaaatgttc catcttcatt caatgggctg
    34921 tttgcttggg ctacgatgta atggtcttcg atatcagctg tcaagtagtc gatttggtca
    34981 gtaacacgac ctgtttcacg gtctacacga cgataaggtg tttcgataaa tccgaattta
    35041 tttactttcg cataactgga caaactattg atcaacccga tgtttgggcc ttcaggagtt
    35101 tcgatcggac acatacggcc atagtgggag tagtgaacgt cacgaacttc ataaccggcg
    35161 cggtcacgag tcaatccgcc aggtcctaag gctgaaagac gacgtttgtg tgtcaattcg
    35221 ccaagagggt ttgtttgatc catgaactgt gacaactgag aagaaccaaa gaattctttg
    35281 atacttgcca caactggacg gatattgatc aattgttgag gtgtcaatgt ttcagtatct
    35341 tggatcgaca ttctttcacg aaccacacgt tccatacgtg ctagcccgat acggaattgg
    35401 ttttgtaaaa gttcaccgac tgaacggata cgacgatttc ctaagtggtc gatatcatcg
    35461 acattgccga taccttccat caagttcaag aaatagctca ttgaagcaat gatgtcggct
    35521 ggacgaaccg tttttacagc tgcttctgga tagccgtttc cgatcacgtt tacttcacgt
    35581 tcagggtctc ttggtgaaaa tactttgatg acttggacag tcattggatc tgtgaccaca
    35641 ccgtcttctg atggatagta agtcactgaa tttaggccat tatcaatgaa aggagctagt
    35701 gtttccatga cttggtgagt cagaactgtt cctttttcaa cgatgatctc gcctgtttct
    35761 ggatcaacta atgtttcagc taatgtcaga ttcaatagac gtgttttcaa atctaatttt
    35821 ttgtttactt tgtaacgacc aacgtttgct aagtcgtaac gttttggatc gaagaaacga
    35881 gcattcagca agttacgtga gctgtctgct gtttttggtt cgcctggacg caaacgttcg
    35941 taaacgtctt tcaaaccttc ttctgttctt gagtcgcttg cgtttttatg caagtctttt
    36001 tcgactgtat tgcgtaatgt ttcgctgtcg ccgaagattt caaagatcgt atcatcggaa
    36061 ccaaatccta acgcacgaac taatacggtt aaaggaatct tacgtgtacg gtcgatacgt
    36121 acataagaga tgtcttttgc atcagtttcc atttctagcc aagctccacg gttagggata
    36181 acagtagaac cgaatccttc ttttccgttt ttgtcgactt ttccatggaa atagacacct
    36241 ggtgaacgga ctaactgaga aacgattact cgctctgccc cgttgatgat gaatgttcct
    36301 tgttctgtca ttaatgggaa atcaccgaag aatacttctt gtgctttgat ttcgccagtc
    36361 tcgcggtttg tcaaacgcaa tgttacatgt aatggtgcag agtagtttgc atcatgcgca
    36421 cgagcttctg ctactgtata ctttggttct ttcaattcat aatctacaaa ttccaaagat
    36481 agattgccat tgaagtcatc gattggcaaa atgtcttcaa acatttctct aagtccctca
    36541 tctaagaacc attggtaaga gtctgtttga atttcgatca aatttggtaa ttccaacact
    36601 tcactgattc ttgcgaaact tctacgttcg cgatgttttc cgtattttac tacgtgtcca
    36661 gccaagctct tcacccctct aaaatgtcat tcaaaaagac tacccgatca attgttacaa
    36721 taatatttca aatattattt ttaggtaaca acagacattt tttgcggctt ttaggcaaaa
    36781 aaaaaccaaa aatagaacat tctcttgaaa ccttacttcg tgagcttcta cttttgtttc
    36841 cttatattaa agccgaaacg gacaatattt cgtttctgcc ttttcttcat cagatagttt
    36901 ttgcaacttc atatactaca cttattttct ataaaagtca acacttcctg gcgtttcttc
    36961 tgctttttcg caacattttc ttaatgtttt caattttatg ttttcaatta tctgacaatt
    37021 cttaattgac agaccttttt atctcggtta tactttagaa aattcatttt gtggaggaac
    37081 ttctatgcga accaacatca accaaatcgc accaacacaa ctgaataact tttactttag
    37141 ctactttaga taggtttgta ttcatcatga tggatacaaa cgttaaacga cagtttgtat
    37201 ctatgatggc taaagaactt tgtattgcag caaaaagcca cgtagacaaa atgctaagtg
    37261 gctgagcagt gaaaatacaa agtaaaagta tcaagcattc tactttttca atttattttc
    37321 cgacttttca atccatttgg acattttgtc tgaatggatt ttttgtttcc caaaattaag
    37381 gaggaaaaag aaatgaaaaa aaatataatc atcggattag gcgctgccct gctactagct
    37441 ttaggaggtt gcgcacaaaa agacaattcc gcagaggcat cagatggaca aaaaacaatt
    37501 ggcgtattgc aacttgtaca acataactcg ttagatgctg cctataaagg attcaaagaa
    37561 ggattagcag aagaagggta taaagaaggc gataatctaa caatcgatta tcaaaatgcc
    37621 caaaacaatc aagacaacct gaaaagtatg agtgagaaac tcgtcaaaga agatcctgat
    37681 ttgctacttg gaatcgccac tccagcagca caatctttat taaatgaaac aaccactatc
    37741 ccgatgactg tcacagcggt cacagacttg gaacaagccc atttagtcac atccaatgaa
    37801 aaacctggcg gcaatgtgac tggcacgaca gatatggtgc caatcgaaaa acaaatcggc
    37861 ttactgttaa atatcgtgcc agacgccaaa acaatcggca tcatgtataa caacggtgaa
    37921 gcaaactcta agatccaagc tgatttggca gaaaaagaat tgaaaaaagc gggcgtcaaa
    37981 gtcaaagtac tgacagcaaa ttccacaaat gatgtccaac aagtgacgaa aagtctagct
    38041 aaagatgtag atggaatcta tattcctact gataacacgt tcgcaagtgc ggcaagtgtt
    38101 gtcggtgaag tcgcaaaaga aacaaaaaca cctgtcgttg ctggcgcagt tgatcaagtc
    38161 aaagacggcg gtctagcaac agtaggaatc gactacgaaa gcttgggcaa acaaacaggg
    38221 atcatggcag caaaaatctt agacggcaag aatccaagtg aacttcctgt cgaaacagcg
    38281 aatgatttgg aactgtatgt caataaagac atggccaaag cattaggcat caatccagac
    38341 agcatcaaag aagctgaata aaacacagga ggattctcat gacaaatttg ctctctcttt
    38401 tcttatcatc tgcatcgcaa ggggtcttat gggccctact tgcgattggt gtatacgtca
    38461 ctttccgtat cttggacatt gccgacttga ctgctgaagg aagctttcct ttaggtgcag
    38521 ggatcgcggc agtaagtatt acaaatggct atcatccatt gacagcttgt cttctaggct
    38581 ttttaggggg tgcggcagca gggcttgttt ccggactgct tcacacaaaa ttgaaaattc
    38641 ccgcccttct tgcagggatc atcaccatga ctggtttata ttctgtcaca tcccgtgtga
    38701 tgggtgcgcc gaacatctct ttattgggtc agccaacgat ctttcattgg gtcgagtcat
    38761 tcggcataac aaaagacaac gctgtactga tcgtcggtct gctttttgct ttgatcgttg
    38821 tatgtttact tgttttgttc ttacgtacag aaacaggact tgctattcgt gcaactggcg
    38881 ataatctggc aatgagcgaa gcaaacggga tcaacacaga taatatgaag atcatcggtt
    38941 atatgatttc taatggatgc attgctcttt ctggctcctt acttgcgcaa aacaacggct
    39001 ttgctgattt aaattctggt attggaacga tcgtaattgg acttgcttct attatcatcg
    39061 cagaagtttt gttacgtaac cagccactat ttctccgtct cttgactgtc gttttaggtg
    39121 cggtcattta tcgttttatt ttagcgttag tcttcgaact gaatgttgaa ccaagtgatt
    39181 cgaaacttgc ttctgctttc gtgctagtga tctgtctttc tttcccgcag atccagcaaa
    39241 aattccattt acctcaaaaa aagaaaggcg gtagtgtcaa atgaatgacg tcttaacatt
    39301 ggaaaatctc catcatacct ttgaaaaagg aacgatcaac gaaaatcatg tcctgaaagg
    39361 cattaatctt tccttagatc ccggcgactt cgtgacgatc atcggtggaa atggggctgg
    39421 taaatctact ttgctgaaca gcatcgccgg tacattttct gtcgatcagg gtcaaatttt
    39481 attgaatgga aaaaatatca cgaaaaaatc tgttgtcgaa cgttcgaaat ccatcagtcg
    39541 cgtattccaa gatcctaaac tagggacagc cgttcgttta acggtagaag aaaatcttgc
    39601 tcttgctatg aaacgaggaa aaaaacgcgg cttcttccgt ggtgtcaaac cacaagaccg
    39661 aacatttttc aaagaccagc tggttcggct aaatttgggg ttggaaaatc gtttatcctc
    39721 agaaatcggt ttgctgtctg gaggacaacg tcaagctatt acgttattga tggccacatt
    39781 acagcgtcca gaactgatct tattagatga acacactgca gcgcttgacc ctgctacttc
    39841 caaaacggtg atggaattga cagaaaagct gatcactgag cagaatctca ctgcttttat
    39901 ggtcactcat gacatggaag acgcgatccg ctatgggagt cgactgatca tgctgcatca
    39961 aggaaggatc gtcgtagata tccagcaaga ggaaaaacag cagctcacag tgccccaatt
    40021 attagcgttg ttcaaacaaa acagcggcac ggcattaaaa gacgatcaag tacttttatc
    40081 ttcataatca gaaagaggat gggacaaaaa atgaacgccc accctcttct tttttctctt
    40141 tccttctttc gtttttgctt tatgtatcaa ccgttgtttc ttcgaaaact attttgttct
    40201 ctccttcttc ctgatagaat ggatgggaaa ggagcttgat gccatgacaa cgaaagacaa
    40261 ggtacttcaa ttattgaaaa agacaccaga ttttctttcc ggagaaaagt tggcacagca
    40321 actggaagtt tcccggacaa gtatttggaa agccatcaaa gaattggaaa aggcaggcta
    40381 tcgttttgag cacgggccta cagggtatcg ttacctgccc tctgacgtac tagatgcaaa
    40441 agaaatccag caagagctac ggtcgtttat tcctgatttg aaagtacaaa cgatgttgac
    40501 ttctgaatct acgatgaaag atgccaaact ggcagcagct caaggagatt ctgtttcgaa
    40561 gttatttgtt gctgatacac aagaacaagc tcgtggacgt tttggacggc cttttttcgc
    40621 tgaacctgga cgaggaatct acatgagtct cttgctccat ccagacaaac ggtttgatga
    40681 attgcctcaa tatacgatca tcgcagccag tgcctgtgtg aaagccattg aatctgtaac
    40741 cggtcgttct gcttcgatca aatgggtcaa tgatatctac atggatggaa aaaaagtctg
    40801 tgggatttta tctgaagcag tcagtgatat ggaaagcggc aggatcagtc atatcatcat
    40861 tgggatcggg cttaattttt ctgttcctca aaatcatttt ccagctgatc tccaagaaaa
    40921 agccatctct gtctttcccg acggacaagc tagtgtcaca aggaatcagc taatcagcaa
    40981 aatctggcaa gaattgttca cttatctttc tcacccagaa aaagcgttgg acttctataa
    41041 agaaaaatca ttcgtattgg gaaaaaccgt gtcgttcaat caaaaaggaa cgacttatgt
    41101 cggtcttgca aaagaaatcg gtgatcatgg cgaactgatc gtccaaacaa atgaacagaa
    41161 gatgagtttg tcttctggcg aaatcagtct cacatctatc cgtacgcaat aattatcaga
    41221 tttcacaaaa ggacacctga tctcatacag tgtccttttt tctgaattct cttcaaaaga
    41281 ctctccttat tctatcagct gacagtttct ctatcctcta gaaaaaggta ttctaaaaat
    41341 atctataaag catggtacac tttctttaaa tgaatcaatg caaaaaacaa gagagtcatc
    41401 agtttacacg atcaaaagag atagcaggcc gcttcctttt tcaatcttat agatagacca
    41461 tgggtaacga taaaaacaaa aaagaacttg cgacttattt tgccgtaagt tcttttttag
    41521 ctagtgcctt aatctcttaa tcccaacaaa cgtctcgcat agatcacctg accagagtgc
    41581 atagcagcat catcaataat agaaacgaga cgcattcctc gtgtgactgg tggtgtccag
    41641 ttctcgtcaa ttacttcatc tagctcggaa ggttccagcc ttcttaagta agaaatagca
    41701 aaatcagcag ctcctcgcaa gtatcctagc agatcttcta tttgattagc ttggatctgc
    41761 tttgcttgtt ccagactatg cttccaatct tgttcgttat ctgctatcgt catcggaaac
    41821 cggctttccc agtcttgact atgccaaatt ggagaagttc ctgctaaatc agcaatctgg
    41881 aaatctaatt ctcgagcggt atgccaagcc agccaagtca ttgatttgat ctgcggggct
    41941 ctatcttcca ctggaaaacg attggcctcc tcaatagaga cgccttcaaa caaacgttca
    42001 aaccgatcgt atgcccgatc cacactctcg catagcatat ctaaataatc catttatttt
    42061 cctccttttt atttatccta cttacttttt cctaattcta ggatacctac ttatatgtta
    42121 ctacacaccc ttatttttta cactttaaca gtcaccatca tcaatagttt ggagattgaa
    42181 agaaaaagat agtgtcgaac tatcaattga acaagaagca acaatatgaa cgtttcatta
    42241 atattcttta aataaacaat gatttttcaa acgaacagtt taagactagg agaaaaagag
    42301 tataatagga aaatgtttga aaggaaagga aggggaaaaa tgaaaaaaag ggcaagttta
    42361 ctagtcctag ttgctttgtt gttcaatatc ttttcgtatg gtgtggccgt ttcagcggaa
    42421 acatacattc ctaaagaccc tgggaatatt tataatgatc taactggtaa ctatggcttg
    42481 ttaggaattg ctagtcaatt ccacgtattt gctaaaaata agacaactgt gaatgcacat
    42541 acagacggaa atatagcaac acatgaactt gatgcaaaaa ataattttgg tacagagctt
    42601 catgaaggtt tattgaacca agaaataaat tatatacaaa aaactgattc tttcgctaat
    42661 tcttcaggca tacctacagg tagcacaaga atgaataaat ttgtcgttgg cagtaacgtg
    42721 gatgtagcac ttcaagatgg tcatcctaca gtcaatggat ccaggcttga tcacttaact
    42781 ccagatgatt tttataaaga tcgtgctggg catgaatata ttgatttcga gcaggaattt
    42841 gctaaattga atgtagcagc aaatgatctt gcaacgatca ctccagctaa aacatatact
    42901 gctgcagatt ttccagacat gaacaatcgg acaatcgatt taactgattt atcagatacc
    42961 gggtttttat tggtaaatat cgatgcagaa gtgttaacga tgaatacgcc acttcaaatc
    43021 attaatccaa atgatcaagt agttgtattt aatgtcatca acagttcttc tgcattgaac
    43081 gtacagtcac caatcaaata caatgatcgt tcaaatcatg aaacagaaga tttttcggat
    43141 gcgaatatct cttggaattt tggaaatgag atgactgatt tgacaatcag tgctcctttt
    43201 caaggaacaa tccttgcgcc taatgctact atccgcatta ctcaaaacca agacggaacg
    43261 attataggaa caaatgtgat tttagatgca gctaccaatc gttgggatcc taacgaaata
    43321 tttatcagcg acaatggtac agatacgaca gacacaagcg acagtacaga tacatctacc
    43381 acaagtgata gctcagattc atca
//
LOCUS       DACCCS010000018        43120 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.018, whole genome shotgun sequence.
ACCESSION   DACCCS010000018 DACCCS010000000
VERSION     DACCCS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.018"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            228..512
                     /locus_tag="IVK82_001036"
     CDS             228..512
                     /locus_tag="IVK82_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP6392112.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(793..1212)
                     /locus_tag="IVK82_001037"
     CDS             complement(793..1212)
                     /locus_tag="IVK82_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP6392113.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1214..1798)
                     /locus_tag="IVK82_001038"
     CDS             complement(1214..1798)
                     /locus_tag="IVK82_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP6392114.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1845..3320)
                     /locus_tag="IVK82_001039"
     CDS             complement(1845..3320)
                     /locus_tag="IVK82_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP6392115.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3501..3812
                     /locus_tag="IVK82_001040"
     CDS             3501..3812
                     /locus_tag="IVK82_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392116.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3927..6263)
                     /locus_tag="IVK82_001041"
     CDS             complement(3927..6263)
                     /locus_tag="IVK82_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6392117.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6405..7073
                     /locus_tag="IVK82_001042"
     CDS             6405..7073
                     /locus_tag="IVK82_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6392118.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7076..8119
                     /locus_tag="IVK82_001043"
     CDS             7076..8119
                     /locus_tag="IVK82_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6392119.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8356..9237)
                     /locus_tag="IVK82_001044"
     CDS             complement(8356..9237)
                     /locus_tag="IVK82_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6392120.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9396..10124
                     /locus_tag="IVK82_001045"
     CDS             9396..10124
                     /locus_tag="IVK82_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP6392121.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10280..10597
                     /locus_tag="IVK82_001046"
     CDS             10280..10597
                     /locus_tag="IVK82_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6392122.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10590..11198
                     /locus_tag="IVK82_001047"
     CDS             10590..11198
                     /locus_tag="IVK82_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP6392123.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11214..12044
                     /locus_tag="IVK82_001048"
     CDS             11214..12044
                     /locus_tag="IVK82_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP6392124.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12182..12670
                     /locus_tag="IVK82_001049"
     CDS             12182..12670
                     /locus_tag="IVK82_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP6392125.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12721..13593
                     /locus_tag="IVK82_001050"
     CDS             12721..13593
                     /locus_tag="IVK82_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP6392126.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13769..14131
                     /locus_tag="IVK82_001051"
     CDS             13769..14131
                     /locus_tag="IVK82_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6392127.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14140..14892
                     /locus_tag="IVK82_001052"
     CDS             14140..14892
                     /locus_tag="IVK82_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6392128.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDVGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14906..16708
                     /locus_tag="IVK82_001053"
     CDS             14906..16708
                     /locus_tag="IVK82_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6392129.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16985..18025
                     /locus_tag="IVK82_001054"
     CDS             16985..18025
                     /locus_tag="IVK82_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP6392130.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18077..20221)
                     /locus_tag="IVK82_001055"
     CDS             complement(18077..20221)
                     /locus_tag="IVK82_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6392131.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20578..21951)
                     /locus_tag="IVK82_001056"
     CDS             complement(20578..21951)
                     /locus_tag="IVK82_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6392132.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21951..22346)
                     /gene="rbsD"
                     /locus_tag="IVK82_001057"
     CDS             complement(21951..22346)
                     /gene="rbsD"
                     /locus_tag="IVK82_001057"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP6392133.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22374..23291)
                     /gene="rbsK"
                     /locus_tag="IVK82_001058"
     CDS             complement(22374..23291)
                     /gene="rbsK"
                     /locus_tag="IVK82_001058"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP6392134.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23399..24412)
                     /locus_tag="IVK82_001059"
     CDS             complement(23399..24412)
                     /locus_tag="IVK82_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6392135.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24493..25092)
                     /locus_tag="IVK82_001060"
     CDS             complement(24493..25092)
                     /locus_tag="IVK82_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6392136.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25278..25979
                     /locus_tag="IVK82_001061"
     CDS             25278..25979
                     /locus_tag="IVK82_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6392137.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25989..28700
                     /locus_tag="IVK82_001062"
     CDS             25989..28700
                     /locus_tag="IVK82_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP6392138.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29151..30779
                     /gene="treC"
                     /locus_tag="IVK82_001063"
     CDS             29151..30779
                     /gene="treC"
                     /locus_tag="IVK82_001063"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP6392139.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30839..32020)
                     /locus_tag="IVK82_001064"
     CDS             complement(30839..32020)
                     /locus_tag="IVK82_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6392140.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32272..33795
                     /locus_tag="IVK82_001065"
     CDS             32272..33795
                     /locus_tag="IVK82_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP6392141.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33864..34592)
                     /locus_tag="IVK82_001066"
     CDS             complement(33864..34592)
                     /locus_tag="IVK82_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6392142.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34605..35885)
                     /locus_tag="IVK82_001067"
     CDS             complement(34605..35885)
                     /locus_tag="IVK82_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6392143.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(36026..36628)
                     /locus_tag="IVK82_001068"
     CDS             complement(36026..36628)
                     /locus_tag="IVK82_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP6392144.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36873..37079
                     /locus_tag="IVK82_001069"
     CDS             36873..37079
                     /locus_tag="IVK82_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392145.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37391..38110
                     /locus_tag="IVK82_001070"
     CDS             37391..38110
                     /locus_tag="IVK82_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP6392146.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38191..38874)
                     /locus_tag="IVK82_001071"
     CDS             complement(38191..38874)
                     /locus_tag="IVK82_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP6392147.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39092..41278
                     /locus_tag="IVK82_001072"
     CDS             39092..41278
                     /locus_tag="IVK82_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP6392148.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            41624..42034
                     /locus_tag="IVK82_001073"
     CDS             41624..42034
                     /locus_tag="IVK82_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP6392149.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            42048..42404
                     /locus_tag="IVK82_001074"
     CDS             42048..42404
                     /locus_tag="IVK82_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP6392150.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        14060 a         7588 c         8621 g        12851 t
ORIGIN      
        1 ttgaacaccg tttaaggaag aggttgtgag cctgccgttt aactctgagt cacaaggagc
       61 aatttcaaaa aacagcttct catgtttgtt gactattgtg acttgtgacc gaggagttgg
      121 caggagaaca ccgtttattc gaaaaaagga actgcgacaa gacttttgtc acagttcctt
      181 cttttttttg ctatgctata ctagatgtaa aaaataggag aaataaaatg agggaaattt
      241 attcttttga atcaaaaatc tatgcttccg aagtcggcaa gggcggggca tatattattt
      301 ttccgtatga tataagaaaa gaattcggtg ctggaagagt taaagtccaa gcaacttttg
      361 acaatgtgcc gtatgaaggc agtatcgtga atatgggcgt gaaaaatcca gatggttcag
      421 tctgctacat ccttggacta cgaaaagata ttcgtaaaaa catcggaaaa gaaatcggtg
      481 atattgtagc agttacagtg aagcaaaaat aaaagaggtt gtgacaaaag acgtgtcaca
      541 acctcttgat aaacggtgtt caaaagccag tttctcggta gtaagtcaaa tttatgcaaa
      601 aatgtggaaa cccatgttta caaaattttt cttactactc gaaacgaagc ggctttatca
      661 caacctcttg ataaacggcg ttcaatcagc tgtccctcgg tattagctca aaaacggcaa
      721 aatatgagaa gctatttttc catttttttc tctcatactc aggtcataac gctgatttca
      781 caacctcttc tttcataatg gtttttcacc atctttataa taaactggtt ctttgatatg
      841 gaaatctatt ttttccaacc aatctaaaat cttgtaacct aactcttctg acatcggaaa
      901 agaggcgttg atttgtgcat tggagatatg gtcaacatgg ataccggaag tgatcgtaca
      961 acttcccgga acggcagttt tgatgatcac agctattttt tcggctaaga catcgtcttt
     1021 atggaaccga ccatcgtggc tagggaaacg tacagtttgc atctctgtat cacgagaaaa
     1081 tgtagtgact gttccgatat gtggtgtatc tccgcctgtt aaagtgatca ataagtcttt
     1141 accgattact ttaacagcag cttggatcgt gaaacctagt ttggttactt caactatcgt
     1201 ttctttttcc atattaatca ccctgccaac gttttgaatg ctcatttaca atttcaaatt
     1261 gatccaaaag tttcattgta tggtgattaa caagatcttg gatcgtttga ggctgattat
     1321 aaaaagcagg aattggtggt atgatttgca cacctagtcg tgctaattta gtcaagtttt
     1381 ccaagtgaat ggcatttaat ggcgtttcac gaggaacaat gactaacttt cgttgctcct
     1441 tcattgttac atcagcagca cgtgcaatca ggttgtcgcc taaaccaaca gagatacctg
     1501 cgactgtttt catacttgcc ggtacgatga tcattccatc ggtcaaaaat gatccgctgg
     1561 cgacagcagc acctaaatct ttgtttgaat aatggtaatc aaacaggctt tcgaattcag
     1621 ttagtgataa gtcggattcc agctttaagt tctgttttgc ccatggactg atgattccgt
     1681 gtgtttctat catcggatat tcttttagtt tcttcactag gttgatggca taaatggtgc
     1741 ctgaagcgcc acttacacca atcacgattt ttttcttccg ttgtgccaat tgttccattt
     1801 tccgcactcc tttttatgat gtcaaaacag ttgcagatca ttcattattt caaatatttt
     1861 tcccaatctt ctacttcttg gaattttgca cgtacaaaat gttctttcat atcgaaagga
     1921 actgtaccgt caaagatagt ttttgcactt atcccacgga aacgaataga gccaggatta
     1981 tattctggta attctgatgg gtccaatgga tggttcctca ttcctgggat cacgatgata
     2041 tctttatctc cttggaaacg agtgttgaaa gtccaaacta catcgttcat gtcgaaaata
     2101 tcaacatctt catcgactaa aatgactgtc ttcaattctt tgaaggctga taaagcaaga
     2161 atagctgctt gacgctgaat accttcatcc gcttcgtttt ctttacggat ttgcatgatg
     2221 gtcatcaatt taccaccacc tgcaggagga ttgtagacat ttactacttt tcctggaata
     2281 gctttatcga ccaaggcaag aatacttgct tctgtaggaa taccggccat agatacgtgt
     2341 tcttcactgg gaccaatcgt tgtttgcatg attggattgt cttttcggtg agtaactgct
     2401 ttgactttca ctacttgtac agctggatta gcatccccag tatagccagg aaattctggc
     2461 attgcttttc ctgagttcgt gttgatatct tcttgcattg tttcatttgg catgatttct
     2521 gcttcaatga tgaattcagc acgagcaata cccacttcat cgactgctat accatcaacg
     2581 atttgaatag gttcgttacg caaagctcct gcacaccata gttcattgta gccaagtgga
     2641 gtggtaggtg gttcgaaagt cgtgccaatg gcaattgctg gatctaaacc aatattgata
     2701 gtgataggca ttggtttatt caaggcttcg aattgttttt ggaagtgacc aatgtggcgt
     2761 ccgccaggca tgatgtacat accgatcgta tctttatctt ctagaaccat acgatggatc
     2821 gttacatcgc tcattgtttt ttctggatct gatcctaaga ctacacccat tgtgatgtat
     2881 ggaccagcat cttcaggagt atttgtaggt gcaggcaaaa tgttgcgaat atcgaagtct
     2941 gcatcggtag ctagatgaac gacttcttgt gctggagcgt ctgctttgtc tactttcaca
     3001 ggtttgattg gattttctac agcgtctttc aagaaacgac caagtgttcg ataatcatgg
     3061 tgcaaaattt cacctacacg tttacgacta gccattgttc cgatgttcac acgtgtgccg
     3121 gggaaacctt tgatattgtt gaaagtcata gctggtcctt cttgagtcgg acgttttact
     3181 gtaccgccag caccgatata acggtaaaca ccagataatt ctgcttcagg atcgatttca
     3241 atatctgttt cgtgatattg acctggaagt gtttttagtt cttcgatgac ttttcttaaa
     3301 tcatacggtt gatctgtcat gagagattcc tccttagttg tctttaccta tctatagtaa
     3361 aaggtgaaaa agaggaaaac aattcaactt gttcggtaaa gtattctaca atggaatgga
     3421 tgcgtttttt tgataataga ataaactatg atatacttat cactaaaaga aagcgcatac
     3481 ttagtgttgg aggaaggaaa gtgaaaaaaa tggaggagtc atttcctaaa gcagtaaaag
     3541 tggaaaatat agcgaatatt ttgaaagtta catttgaaaa cggagaagta aaatacgtga
     3601 aatctcattg gacagaagag ataaccgatg ccttgcaatt tggaaaaaaa ggaagaggaa
     3661 aaagaaaaaa tcttctcgca ctttctcgta atatgtggat tgggactgaa gtaacgattg
     3721 aagcagatgg aacagtattc ataaatggaa aagacaggta tacgccagaa gagttgtggt
     3781 acaaaggaaa gaaaagtata ccagaattat aaaaaataat taaaaaaaac agcgcgaaac
     3841 aaggaacata ttacttcctc gtttcttgct gttttctact atttcagcgc tcttatgact
     3901 caaatctaaa tgtggttgtc acaaccttat tctgcacgca aagcgatagc ggcgtctttt
     3961 ttcgctgcca ttcgtgcagg aagatgaccg ccaagcatcg tcaatatagt agagacgatc
     4021 actagaataa tcgcatggac aggatttagt tgagcaacat tttttagatc cgtcatgttg
     4081 tataagatgc tattgatagg gaacgtagct agccatgcaa taaacacacc taatatccca
     4141 gaagaaatac ctaaaataca cgtttctgcg tcaaatacgc gagtgatgtc ttttttacgt
     4201 gcacctaaag cttttagtac accgatttct tttgtccgtt caatcacaga tgtataagta
     4261 atgatactga tcatgatcat acttgttact aaagagatcc ctgcaaaagc aataaggaca
     4321 taagtgatag catccatcaa tccgccagtc agttcagtca tcgttccagc taaatcagta
     4381 taaatgattt tatcttcatc tgatttgcct ttattgtaat catctagata atccaagatc
     4441 ttttctttgt cttcaaaatt gttcggataa atcatgatac ttgaaggcaa gctgtctcca
     4501 cctaaataac taagcaatgt ttgtttggca ttttcgtcaa ctttttcagt agttaagaca
     4561 tttacaccac tgtctctttg agctttgacg atttctgatt ctttgttttc gttgacgatt
     4621 tgagtcgtta attgatcgct ataagcgatt cctggagata agagattcat tgtagaagaa
     4681 gatttcacgc gtaggatccc cgagatcttc aattcatcag aagcattctg atacatcgca
     4741 tcataatctg tattagggat aaaattccca gttggaagtt ttgtatagta agtattgttg
     4801 tttactaatt tgaatgttgt accaacgata tcatcaaaat caagtttttc gtcttctttc
     4861 acatcaaatc caagattctt caatgcgttg atattagtat tattattgcc gtctacgatc
     4921 agcacgacat ctgttgctga ggctggataa cttccggcaa gtaaagaata attgtctttt
     4981 aaaaagtttt ctttgctagt gtcaagctgt gtagggaaac ttgacactcc cacacctgtc
     5041 attgctgaca tcgtactaga taaagaaaga gattccgtat caggattttg attcgagaag
     5101 ctgacaggct gtactttgcc atttacatca cgtaaaagat tgattcctgt agtacgggta
     5161 aatccgatat tattacttaa gttaggatcg atgtctgtta cgtaatcgac gtatgtttga
     5221 tcaatcttat tcgtgtgctg tgctttatct tcttcgctga cttttgcagt aatcgttttg
     5281 ctgtctggat aatccgcttt gcttgcaccc aatccggcat cgtcacgagt ctggttagtc
     5341 gttaccttag aaatagtaat cggaaatttc gccatcgttt cagcttgcgt gttatcgatt
     5401 tgtttttgga atccagaaga aagggacaga acgatcgcga ttccgatgat cccaatactg
     5461 gaagcaaagg aggtcaagaa agtacgtcct ttttttgttc ggatattgtt gaaggaaagc
     5521 tttaacgctg tccaaaaact catctttgtt ctgcgtaagt taaattgatc atcttttggt
     5581 cgttcgatgt gaggatggga atctgttaag atcttaccat cagaaaattc aatgattcga
     5641 tctgcgtatt gatgggccag ttcaggatta tgtgtgacca tgatcactaa tttttctttc
     5701 gataactctt ggatcaattc catgatctgg atacttgttt ctgtatctaa cgctccggtt
     5761 ggttcatcgc acaaaaggat atctgggtca ttggctagtg ctcgagcgat ggccactcgc
     5821 tgcatttgtc caccagataa ctgatttggc tttttatgca tatgatttgt caagccgact
     5881 cggtgcaatg catcttctgc ccgttttcgt ttttcgtctt tagaaacacc acttaatgtc
     5941 atcccaagtt caacgttttc aataatgcct agatgaccaa tcaagttgta actttgaaaa
     6001 atgaaaccga tagagttgtt tcggtaggca tcccagtcac tgtccttaaa gtctttagta
     6061 gactttccgt tgattaccat atcaccggaa tcataattat ctagtccacc aatcacattc
     6121 agcatagtcg ttttaccaga accactagga cctaaaatcg ctacgaattc tttttgtctg
     6181 aaagcgacag acacaccatc caatgctttg gtggtggttt cgccgacttt ataatatttt
     6241 tttatatctt ttaattgaag cataatcacc cttctcccgt attgaaatat tcggtatact
     6301 aataagtata tcagaacaat gatcgtagta tacgaaataa atgttaactg aagatgaact
     6361 acaagaaaaa atctagaaat ttttcaaaaa acagagggag atcaatgaat cgtattttag
     6421 tagtagaaga tgatccgcaa ttacagttgc tttaccgttc tgtgttggaa cgagcgggct
     6481 tttcagtcat caccgcattg aatggagagg atgcgctgaa aaaactagaa gcgacacgta
     6541 tagaattaat cattacagat atcatgatgc cgaaaatgga tggctatgac ttgctagact
     6601 cattacgtag caccagattt gacacacctg ttctaatgat tacggcaaag gccgattttg
     6661 aggataagaa acgcgggttc aaattaggag cagatgatta tatgacaaaa cccatcgatg
     6721 tcaatgagat gatcctacgt gtggaagcat tgcttcgacg ttcaaagatc aattacagca
     6781 gtagcttgat aatcggacaa acaaaattag agctagatgc ttatacagtg ttacaagatc
     6841 agcattcgtt gattctgcct caaaaagaat ttctgcttct gtataaatta ctgtcttatc
     6901 ccaacaagat cttcactcgc caacagttga tggatgatat ttggggatta gatacgaata
     6961 cagaagaacg aacgatcgat gtacatatca aacgtttaag gacccgtttt gaaaagacgg
     7021 aagattttca aatcatcaca gtacggggac taggctataa ggcggttatt taaagatgaa
     7081 aaaaaagcta ttttcacaat tatggatcta ttttgctagt attgtcttca tttcgatctt
     7141 agtgacactg ctgtgttttc tcggttttat tttcttattg tctcgacatt ctatctcgcc
     7201 tgctgaatcg gctggaaaac caacgttata cccgttattc gtctttgcgg ggttcagtat
     7261 ggtcgtgggt acaggaatat cgatttttgt tgggcggcgt atcctgcatc caatcagtgc
     7321 gctagggaca aatatgagtc tggtagcaac gggagatttt tcgattcgaa tggatgaaca
     7381 gcagaaagta gcagaagtac agcaactgta taaagatttc aacgttatgg tccaagaact
     7441 caatagtatc gagacactga ggaatgactt tgtgtccagt gtatcccatg aattcaaaac
     7501 accactagca acaatccaag gatatgttca attgttacaa gccccaaatc tctctgacga
     7561 agaacgccaa atatttttat atcggattat cgaaagtatc acgcagcttt cacaattaac
     7621 agagaacatc ttaaaattga acaaactgga aaaccagcgt atccaactgg aaaagaaaga
     7681 gtatcgatta gatgaacaaa tcagagaagt aatcgttttt tttcagccta aatgggagaa
     7741 agaacagcta gaattggata tcgaacttgc tgcagtaaat tacacaggca atgaagagtt
     7801 tttgtaccaa gtgtggctga atatcatgga taacgccata aagtacaatc aaatcaatgg
     7861 acagattcat atcaaactat ttgagacagc cacagagatt gtactggaag tgaccgattc
     7921 aggtgtagga atgaatgaag agaccagaga tcgaatgttt gaaaaatttt atcaaggaga
     7981 tacaagtcgt caaatttctg ggaatgggct aggactttca ctggttaaaa aaattctaga
     8041 actgcatgat ggacgtattg attatagcag tatcgaagga gtaggaacga cagcaatgat
     8101 acggttaagt aaacaatagg aaaaagaata aaacccctaa aaattctatt gaaggttttt
     8161 aggggttttt agtcgttttt ttgtgattag ggaaatgttc ttacggttct tttggcataa
     8221 aaatcgctgc tttcttattg aactacgaga aatgttagag gtgtcattga aacaaggaca
     8281 tgatgcaaga aagagagcga aagtcatgcg tcgctctctt tttttgggtg gatagatgga
     8341 cagaaaatgt agggcttagt tttcttcttg atttaaatac tcccaaaatg tatctgaagc
     8401 caaagaatgc tcaccgactt tacgagtaaa tgcaagataa ctctctgttg ttggagaaat
     8461 agggagaatc acagtatttt cttttaatag attcttgact gttttttcca taacaatagc
     8521 aatccccatg tcatttgcaa tcatattgat gatcaagtcg attcttgctc ctttataaga
     8581 gatttttggt tcaaatccag cttcgcgaca cagatctagt acaggttgat acaagttcgt
     8641 agtggggcca aggagcaaaa agttctcctt tttcagttcg cttagatcca atttcttttt
     8701 actagctaaa ggatgatttt taggtagaac tgctacaaaa tcgtctgttt cagtaggcaa
     8761 ccactcaatc gaacttggtg gggcctggaa acttctagca aagtggatcg tgtcactatg
     8821 ttcattggcg tcattgtaag ttaattcgac actttccact tctttcaact ggactgtaaa
     8881 ttcaggatgg cgtttcaaaa actcggtgat tttggaaaaa cctttataat tgatcattgt
     8941 cggtatcgtt aagatattta atgaaagatt gttgtctttc gtataggttt caagcacttc
     9001 ttgcatagca tggtattgtt ctacgacatt ttttacatat gggagcaata agcttccagc
     9061 ttcggtcaat tcgatttttc tatgggcgcg ttcgaataat ttgacgtcta attctgtttc
     9121 taacgaaaga atctgtttag aaatatttcc ttgggtggtg aattgtcgat cagcagtttt
     9181 ggtgaaattc attgtttcgg ctaaatcaag aaaaacttcg aatttatgga tatccatcat
     9241 attcctccta cttattccaa taaagaatac tttaacacaa aaaatgaatt ggtgtaatct
     9301 tctctgtctg ttacaatctt tcttgcagcg aaagcaataa ttacagaaat ttttctgatt
     9361 tttttcgctt cgtagaaaaa gagaggagtt ttttgatgcc aactggagtt ttgatcaatg
     9421 taggttccgt tttgtttggc ggactaattg gcggattagt aggaaataaa ttaagtgaac
     9481 attttaaagc acagctgacg atggtttttg gtgtgtgttc gatggggatg ggaatctatt
     9541 ccattgcgcc gatgaaaaat atgccggctg ttatttttgc tttagtgata ggaacagcga
     9601 ttggtttgat tgttcattta ggtaatggga taaataaggg ggctgcgctg atgcaagtac
     9661 ctatttctaa aatcttccct agtgaaaagc ttggtatgac ccacgatgaa tttatcagca
     9721 cattagtgac ggtcattgtt ctattttgtg caagtggtac ggggatctat ggaagtttag
     9781 attctgggat gacaggagac agtacgattt tgatttctaa atctgtcctt gattttttca
     9841 cagcagctat ttttgcgtgt aatttagggt atgtcgtttc tgtgatagca attccccaat
     9901 tcattatttt ttatattttg ttcttattgg caaaatttat ttatccattg actacaccag
     9961 acatgatctt agattttaaa gcttgcggcg gattcttgat ggtcgctacc ggttttagaa
    10021 tgatcaacgt gaaaatgttc ccagtagctg acatgatacc tgcaatgatc gttatcatgc
    10081 cactgagctg gatgtggaca aattggatta tgccactgtt ataataaaaa tcaaagagaa
    10141 gccatttttt gctgactaaa aaggatcaaa tcttttagtc agtttttttg tatttgacaa
    10201 actgattttg aaactatatt atatgttgta taatatagat tgaaataaaa tattacactt
    10261 gagaatagaa ggtgacgtaa tgcacatcca gattccaact gttcttcttg atggtactgt
    10321 gctagcagca ttaagcaagg aagatatgta tgggtatgcc ttaacaaaaa tcgtacaaga
    10381 aagtctctct gtcagtgaat ctacactata tccggttttg agaagattga aaaaagaagg
    10441 atatcttgag acctatgatg aaccttttga aggcagaaac cgtcgatatt actcgttgac
    10501 accaatggga agtcgtcatt tagaagaaat tattgatgaa tggattgttt ttcgagattc
    10561 tttagaccgg ttattggagg agagcacaaa tgaatgaata tttgaatgaa gttgtggatc
    10621 atcttcaaga aattcctgaa gacgatcgtt ttgatttgat ccagtattat gaggaatatt
    10681 ttttagattc tggaaaaaca ttagatcaga tcattgatga atatggtaca ccaaagcagt
    10741 ttgccttaaa attaaaaatc agttactttt cagaagaaga acaagcagaa aacgagttag
    10801 tgaaaaatcc aaaaagacaa attcgcctga tttggttaat cattatcggt ttatttgctt
    10861 ctcctgtcct cattccacta gcactggggt ttatattggt tattggggga ctattggttg
    10921 ctttactagc gctcattttt agtatctatg ttttatgtat cagtatctta ggtgttggcc
    10981 tattcatgat catctcagga atcagtgtgt ttggacaatc gattctgagt ggattattct
    11041 ttatcggtgt cggtctgttc gccacaggaa gcgcagtatt tatttcgcca ctgttgctga
    11101 aaggaacaaa agggcttttt tatgtcatga tgaactttgt taaatggatg ggacgacgtt
    11161 ttattacaaa aagagatctg cattcagcgg gcgtttgaag gaggacacat aaattgagat
    11221 tgaaacattt tttgattacc ggtattggtc tgatgcttac tggtggcctc ctagcaggtg
    11281 caacttatac attaggcgct caaaagagtc tgacttggga taatggtcct cggttgatcg
    11341 atatggttac tgagacaaaa aaaatcaagg atgcaaacaa aatcgtcatc catgcagaaa
    11401 atcaaacagt ttcggtcata cgcggattgg attttgagat aaaaacgact tatgataaga
    11461 cagaaaagcc agaaataaag gagaaaaatg gcgtgttgac tgtgaatgca gtcaaaaaat
    11521 caacaggtgc aatcattagt ttagaagaca caagtgcaca aatcactatt gttgtccctg
    11581 cagatcatga attatctgaa ttgacaatcg aagggaataa tagtgcaatt gatatcaacg
    11641 aagtatcaat caaaaaagtg aatactaaga cacatgatgg ctgggtatcg ttcaatgaat
    11701 ttacaggagg aacaataact gcgaaagatg aattaggctc acttcgatta gatgaggcca
    11761 atgtggataa cttgatcgtc acaggtgaaa acagcaactt ccatctagaa gacagtttga
    11821 tcagcaaaga aggaaaagtc atcctaaacg gtggcaattt atctttgcat gagatggaaa
    11881 acagtggcta tactatccaa acgtctggtg aatcttatat tgaaaaagac taccagccaa
    11941 taacaaatac tttgttagaa caaggacaga aaaagctaaa gatcactgct aaagacacat
    12001 cgatcaatat tacgacggat caagaggaca atgaaacaga ttgatcctgt actcaactgg
    12061 atattttaag aaaaaacagc aaagatcctt ccaatctttg ctgtttttca tcgcttaacg
    12121 cacataacta tagtaaaata agaaagaaaa tatctaaaaa gaaaacaggg tgaaagagta
    12181 tatgaaggta gaagaaggtc aaagagaaaa gttaaaaaca gaaatggaaa gattacatac
    12241 ttatatcact caactaagcc aaaccttcta tgatccagac aaagaaaaag tgatggttaa
    12301 ttacccgaac aattcagaag gtcgccaatt agagcaagtg taccatgaag ttttcaaaca
    12361 tttattgacg gtgaagaaag agctggatta ttattcttta ccgattatcg atacaggaat
    12421 attgaagtat gatgggaaga aggaacgatt tatctttaaa tctgttcggg aagatttgcc
    12481 tctttcagca gggatggatc tagaggtctt ggtagaagac tattttacag aagaaaagca
    12541 ctgggttcga acaaaattgg attatttacc gcaggcagcc ggagggacac aggcaagcgg
    12601 ttggtacatc actgaagaca aagagttaga actagaaggt gtaatggcgc gtattcgaaa
    12661 aaaaaattaa aaaagtaaca ttagatattt attgaaggat aaaggagcgc aggaagtagc
    12721 atgagagaaa aaagagcatt ttatatcaat ggttatgcag ggattgtcgg attggtcgta
    12781 ttagctttag ttggattgtt tttcttctat ttaggtatgt ggcaagcgaa agtttgggca
    12841 ctttttttaa gtatttttct ttggttgatc acgttattgt tgttaagttc ggcaactgta
    12901 gtcagtccta atcaagcaaa agtaattttg ttttttggac aatatttagg tacgattcga
    12961 gaaaatggct ttttccttac gatacctttg gcacaaaaaa tgactgtttc gttgaaagtt
    13021 cggaatttca atagttctgt tttaaaagtc aatgatttgg atggaaaccc catcgaaatc
    13081 tcagcagtag tagtctttaa agtcgttgat acagcaaagg cattattcga tgtagcgtat
    13141 tatcaagatt ttgtagaaat ccaaagtgag acagccattc ggcatatcgc tagccaatac
    13201 ccgtatgata cattcaatga gaatgatttg acgcttcgag gaaacacgac ggcagtgtct
    13261 gacgagttgc aaaaagagct gcaagagcgt ttagcagtag caggtgtcga agtaatcgaa
    13321 acacgcttga accatttagc ctatgcaaca gaaatcgcta gtgccatgct ccaacgtcaa
    13381 caagcaaaag ccattttgtc tgctcgacaa accattgttg aaggagctgt cacaatcact
    13441 caaatggcct tagagcaaat cgaagatgga caagaaatca atttcaccga tgatcggaaa
    13501 gtccaattga tcaataattt acttgtctca atcattacag acaaaggaac gcaacctgtt
    13561 atcaatacag gcgacatgaa agaccgtaag tgacatactt tttgtaagat aggaagaagg
    13621 agtttaggac ggattattgt cttgaactct ttttttagtt gttttaaaga aaatgacaaa
    13681 attgaaagaa gaatgatagt tgattcgaag gcagaattgt tcgctttttt ctataatcag
    13741 tatataaagt aagagagaag gggaaaaagt gaaaaaactt ctgatcggtc tacttgtttt
    13801 catcgttgtg gtacttgcag gggtgaaaat cgcagattac gtagttatgg gtggagaaga
    13861 atactatgta caaattacga cagatggtac aaaaacagtt tcaaaatcag actctggaca
    13921 agaaacagtc aactatagat atatcctgcc tggctatacg aaagagggta aggaaaaaga
    13981 actggacttc aagggacaat tgactcgacc gcttcgtaaa ggagcatatt taaaaatcac
    14041 ctggaacaaa aataaaggtg tcacttcgta tgaagaagtc gaaaaaaaag acgtgccgaa
    14101 aacagcactg gaaaaactaa ttaaagggta gggaaagaaa tgaacaaaat agtcgaagta
    14161 cagaatgtaa caaaagtata cggaaaaaac aatgagaaaa agacgcaagc attaagcggc
    14221 atcagcttcg atgtagaaaa aggcgaattt attgggatca tgggcgcttc tggttctggt
    14281 aagtcgacac ttttgaatat tttatccaca ttagataaac caacagatgg gcatatccga
    14341 atcaatcaaa aagatgtgac cacattaaaa ggaaatcaac tagcagattt tcgtgcgaat
    14401 gagattggct ttattttcca agatttcaat ttattagaaa gcttgacagc acaagaaaat
    14461 atcgctgttc ctctttcttt acaaggcgtc agaccaaaag aaatcaagca acgtgtccaa
    14521 aaaattgcag aacgtttatc catctctcat attttggaaa gctatccttc tgagatttcc
    14581 ggtggacaaa aacaacgggt cgccgccgca cgtgcattga tcacacaacc aacgattcta
    14641 ttaggggatg agcctacagg tgcgctggat tccaaaagtg cccgggatct tttagatacg
    14701 atggacgaat tgaatacaaa agatcatgta tcgattcttc ttgtgacgca tgatccgttt
    14761 tctgcaagtt attgccaacg tattttattc atcaaggatg tcggtatcca tcaagaagtg
    14821 aaacgtggag atcaaagccg tgattccttc tatcgagaaa tactatctat tcttggaaac
    14881 ttggaacagt agggggagat tgactatgct ttggaaatta tcacttactg ggatcaagag
    14941 ccgtttaaga gactacttgg tgttattctc aggattagtt atggcatctg ctatttttta
    15001 catgtttgaa tcaatggcaa gcaacgaagc ttttttgaaa agtaatacga tcgttgcggc
    15061 tacagttatc attttccgat tcggttctgt tttactaggg atcatcacat tcgtttatat
    15121 tttatatgca aattcctttt tgatgacgat gcggcaaaaa gactatgcaa tgtttatgat
    15181 gctgggagca aaaggcagaa aaatcgcaca gatgattttt tctgaaacat tcttagtagg
    15241 gatcgcagct actttagtgg gttcagctgt cggtgtagga ttgacaagta tcgtccaccg
    15301 attattagtg gatcagttag atctcaatat ttcgcatttt acacctttta gtattcacgg
    15361 gttgttgatc actgttttgt tttttgctgt tttgttttta ttagctgctg ttgtcaatgc
    15421 tttttcgatt gtcaaaaaac cgattttagc attgattcgt gcggatcaga caccaacaag
    15481 aatcaagcag cataaattct tgtttttcct tgaagtagta ctgggaattg cttttctagg
    15541 ctgcggttac tatatgatga aacacgtggg gacctatcaa gtctttggta taggggtagc
    15601 cttagttacg atcgtattag gaacttattt cattttccat tctgtgatca ttttcttttt
    15661 atcattacta aagaaatcag aacagatttc attgaaaaaa ttgaacaatt tcacattgtc
    15721 acagttaaac tttcgtattc gtgaatatac acaaatgctc tcaatggtcg ctatgctgtt
    15781 tgccttggca ctgggcgctt tgacggtagg attaggtttt aaaaatgaaa ttgcagactt
    15841 gacagaaaac aattctggat atgatctagt gatgaataat cctcgggcag aagatcagca
    15901 aaaaatcaaa gaactttcac caacactttc tgcttcttat actcaaaaag aagatgcggg
    15961 aacgatttac tataatgcaa gtgaatttgc ggctcaacca ttgatcatga taaaacatga
    16021 agatggaaca ccgccgaaga tcacgaaaga aaaagttcct gctaaaaaaa tgaatgaact
    16081 gactgtacaa gaggaacttc gaagctatga attgccggaa cagcaagaaa aagaaatcaa
    16141 ggtagtcagt caatcagaat ttgatcaact gaacttgcca caatccactt tacagctagt
    16201 tcaggtaaaa gacttccaag caaacctgaa accaatcgaa acacttgttg ctcaaaataa
    16261 aatgaacaat ccatctttgc aaaaagtaga gtacagcaac cagaaatatg aaatgtatga
    16321 agtgtataat ggtctattct ctggttttga atttatggga ttcttcctag gtttagcttt
    16381 cttgaccatg cttgctagct gtttgatgtt caagatcttg tcaggaagca agagtgacat
    16441 tgctcgttat gaaatgcttg aaaagatagg aacacgacgc gggttgctta aacaatcgat
    16501 ccgtagagaa gtaggcgtgt tattcttagc accaggaatc ttaggtgtag tccatgtctt
    16561 attcggatta caaatgtttg gtctattcat gcaaaatcca tacaaagata tttgggttcc
    16621 gtttacgata ttcttcgtat tgtatttcat ctactatgta ctgacgacat ggttatatac
    16681 tggaatcgta ttgaaaaaga gattgtgaaa aagctgtcct gcatcaagta ataagaacag
    16741 ccaagcaaaa atagctcctc atatttttcg tttggttggg cttattaccg cagatgcaag
    16801 cttttgaaca ccgtttgttc ggaaaaagag gttgtgacaa tatttttgtc acaacctttt
    16861 tttcacggtc ttttattaga atatagtaaa ttttttctcg ttactcacgg aataagctgt
    16921 taagaaaacg gtttccatgt atactggaat cagtgcaaat gaaagaaaag agggtgcaag
    16981 gaacatggaa atcaatgaac aaaattttgg cgaaaactat cacttgatta cgatcacgaa
    17041 tcaaaatggg acatctttgt ctgtcagtga tcttggggca cgggttgttc attttcaaac
    17101 aataatcgat aatgaaaaac gagagttgat cttaggtttt gatgaagcaa aagaatatct
    17161 agaaaaagac ccttatatcg gtgcttcaat cggacgtatg gctggacgga ttacgaatgg
    17221 cacattcaaa ttagatgatg aaacgttcga agtaaaaaca gacccggaca ctggtcattg
    17281 tttacatggt ggtgcaccta gctttgaagc caagcagtgg cattatacag tcatcaatgg
    17341 tgaaaacgaa gcatcggtca ttttctatca gactagccca gatatggaaa acggatttcc
    17401 tggaacttta gatgtagaag tacggtatac gctgacgaac gatgatacat ggcgagtgac
    17461 ggtccaaggg atcagtgacc gcaaaacagt attcaatcct acaaatcatg tctatttcaa
    17521 tttaaccggt gatccagcac agtcaatcga cgaacatata ctttggttga atagccgatt
    17581 atttgctacg ctcaataaag atacaacgcc tacaggagaa gtagtttcag taagtggcac
    17641 agcatttgac ttccaacgtc ctaaaaaatt aggtgaggtc tttgccagtg attttcgcca
    17701 atcaatgata gtgggcggca tcgatcatcc atttttgttg aaaaaacaat ctggtttact
    17761 aaatgctgcg gaattgacaa gcccagatga aaaagtatcg atccaagtaa aaacagatgc
    17821 ttcaggaatc gtgattttta cagcaaattt tggagaagat ggaccggaaa tgcgcgggaa
    17881 taaattagta aatcatggcg gtatcacatt tgagactcaa gaactgccag gagcggagcg
    17941 acttgaaagt tttggagatg tcactctgga acctaaccaa gtacgcgagt ttgtaacgga
    18001 atacaaaata aatataaaga aatagaataa attaaaaagg gagtgtgaca gtgtcacatc
    18061 ccctttttaa tttattctat ttctttaaag gcagatcaag ccagaatgat ttgtctaaac
    18121 ttaacaaggt tagtacagga acagttgcag ctttaactgt acgagtagtt gtttctggta
    18181 tctgtcttgc cgtaaaattg ttccacaaat cccagattga agcgacacgg aagatggaag
    18241 aagcgttgtt gacgcgtttt atcgttgaaa attctaatcc tgtgattggc acgccatctg
    18301 ttgtccattt gattggcaca tcgaatcgct tagacatatt gaggatttca gcgtttgctt
    18361 cttcagttgt tgctgcttct tttttcggtt catcatattt tgtcaggtcg tacgcttcga
    18421 tgatcgcgtt caatttttgt gaatattggc gatctgtagc ataacgtcct tccaaatatt
    18481 tggttgcttc tttgtagtct tttgtttccg acttccaagt tggtttgtag aaatcaggat
    18541 tgccagtcag tccatttttg atcaagtcgg catagtcttc caaactttcc ttgtaagaag
    18601 gatatttacg gaattgtgcg cggatttggt agctttgtcc ggaactatct tgttcaagtg
    18661 tattgaaggt cacgctgctt cctttatatg atcctttgat accaaaaagg ttatgatttg
    18721 gttcgcttga taataagctg tttccagatc cactttcaag gatagcttga gccatcatga
    18781 ctgatgcgta gattccttct ttttgcccga tttcctgtgc atcatctgca atctttttca
    18841 agaactgatc agttgtttgg ttttgactga aatggaacga gccatctaat ccatctaaaa
    18901 tagatgagcc gcctgtcagc gaacctgaag cagcaggtaa acctgctaag tttactcgga
    18961 cagcaaaatc aggcgtgaaa tctgaaatag aagttactcg aactttatct cctggttgag
    19021 gcgcatggat aaattgtccg ttcccaatat agatacctac atggtgagta acaccggaag
    19081 aaggtcccca gaagacgagg tcgccaggtt gggcctgact tactggaatc tgcgtacctg
    19141 catattgctg ttcgcctgtc cagccaccga tataatgacc ggttgctttc atatatacgt
    19201 aataagtcaa accagaacag tcaaaagtat ttggaccttt tgctccccat acatatggtt
    19261 ttccaagatg cttgaatgct tctcgaacga ttgcttgttg gacattcgtt gtatttgtag
    19321 gcaatacagc atcaattggt gattttacaa caaaatcaga agaagcagaa gatggattct
    19381 ctataccgct ttgatttgct gtatttggac gagatgaagc attgttcgtt ccgccattcg
    19441 atgaaccatt gtttggatgg tttggttctc tctgagtatt tccattgtta ttcgatccag
    19501 atgggtttgt ttgattcgaa gaattcccgc tgttcgaatt attgttatta ttgtttgaat
    19561 tatttgaacc attattattg ttgttattac ccacatctgg tttgtttggc gtttccggat
    19621 ctggtgtact agggacttct tcactaggat cagttggttt ctctgttgaa gtatccggtc
    19681 cagctttaaa ttctgcagta atagataaat tgttcgtacc tactgtaaag gtctttgcat
    19741 cataatttac ttctttggca ccattaacag cgactgaagc aatttgttca ccttcagctg
    19801 gcgtaatagt gtaagttacc ttcgtttccg ctaataaacc ttctaccgtt ccactttccg
    19861 taatatccaa aggttgctgt tcaggggaat ccgctgtcga agcagtcagt tggatcttac
    19921 caatcaaaga aggagcaatt gtaagggaat atgtctcggc tggcttttca ttttctgatg
    19981 gatcagccgg atcaatttca gttggctcgg tcgatcctgt tgttgagtta gttgttccag
    20041 ttgggtcttc tgtaaaagaa tccgttgttt cactagtagc tgagctggat gtagtcgtat
    20101 ctgttgtgac tgtttgatca gctatttgat tatctacggc aaatacggta ttggtcgaca
    20161 aaacagacgg agcaatcagc caagtagcag ctagcaaacg tatcgttttt ttatttttca
    20221 taataaacta gtccttttcc tttcattgat tttctgtctt attcagcatt cattagacat
    20281 tttttggaac agataatcgt gtcgaaaagc tttttctttt cataaatctg tctgtgtatt
    20341 ctaaataaaa caatttgtaa ataagcaacg agcattcatt cgtttttctt tgacgttcat
    20401 tttattgcat agaccacctt tgtttgggga taaacaagtg gggataaaaa tggttatgca
    20461 tatgtaaatt ataccaaagt ttggaaagta tcacaaaaat ttataaaaat aatgatccta
    20521 aaaagaaaaa gactgaaata tgtgataaac atctcatact tcagtctttt tcattaacta
    20581 ttcataagac attaggtctt catcaaaccc gctaacgaga gtagtgatca ttgctacgac
    20641 tagactaaca gccagtcctg ctaagtacca gaaaaaagta ccggaaatga atgcaggcaa
    20701 tgtggtcaga ctaccaaata caaaagcgat cgctttcact tgggcaaatc ccattacagc
    20761 tccaccagca gctgcaccaa tgcaagcagc aacaaatggt tttttcaatg ggatcgcaat
    20821 tccgtaaaca gttggttcgg tcactcccgc aagtaaaatc gaagcaaaag cagaaccgga
    20881 gatcgaacgg gtttttgaac gtttacttcg cagaaataca cctaaagctg caccagccat
    20941 tccaaaattg gctgctcctg tcaacggaac gattcggtcg aacccataag cagagatatt
    21001 actgatcatg acagggttta cagcccattg gataccaaat attaccaaaa tattccaacc
    21061 accagcgatg attgcgccac tgatcggtcc gttaatatcc atcatccaag taattgcggc
    21121 agctaaacct tcgccaaggt aaacacccgc aggcccgaat accattaacg tcaaaggaac
    21181 cgtgataact aatgaaagta aaggtacaaa tactaattgc atattttcgc gaatatattt
    21241 tttgaggaat ttttcaacaa aagaataaaa gaaaatacct aaaatcgccg gcatgacagt
    21301 agacgtatac tgcatcagta ctacaggaat ccccagaaaa tcagtagctg ctttgccact
    21361 gtctgaaagt aaagaggtca gattcggttc aatcagtgct gcagcaatga cagccgcaat
    21421 atatccattc actttaaacg ttttggcagc agaaaaagca agaaagattg gcagaaaata
    21481 aaagacggca ttcgacgcag cagacaaaat atgataagtt ccgcttgttt cactaagcca
    21541 gccaatcgta gtcgctaaaa caaccagacc ttttaatata ccagaaccgg caaacagcgg
    21601 aatgatcggc gtaaagattt ccgatatcaa accaaatagt ttttcgatcg gatttagctt
    21661 ttctttcgga gctgaatgat cgatttgacg cgtcaattcc ttgtaagcag gagccaatcc
    21721 ctgatccatg attatttctg catatctatc gagtaaaatg aaatacttaa tggaagggat
    21781 ttgctgtttt aatttttctg cattggctac tggctgtttt ttcaatttga tacgaagccg
    21841 agtcgcacat ggaaggacag aatcaatatt gtcttttcct cccaactgtt caatcagcat
    21901 atgaatctgc tcggacagta ccatctgttc aggattcatt gtcgtttcca ttagaagacc
    21961 actccactca ctaatataat attggcgtaa ggtgtcattt cgcctgtacg aataaatgca
    22021 tgacagttat tcaactcttt tttcatttct tcatgtggga taaagctaat cggtgtatct
    22081 ggtaacaata atttgatttt ttccaatatt tccgggttca ttgtctttat ttcttccgcc
    22141 aattcaatcg attgtacctc aagttcttct aaaacgtttt ctaaaacaga taaaaaactt
    22201 ggaagaccgt ttgagacagc caaatctatt ttttcagtac caaaggggac aggcatgcct
    22261 gcatccccga tacttaattt atcaaagtgc cccatttgtg cgattactcg tgaaatatct
    22321 gaattgatta cttttccttt tttcatgttt gtttcttcct ctcattgtta attttagact
    22381 gctgattgtt ctaaagcttc gcggtaaggg atagaaggtt gcgcaccata agactgcaca
    22441 gcaacagaag aggctagatt tccataaagg atcgcttctt tcaagttact aaggtctggc
    22501 tgaagtgtgc tgcttaatgc gccaataaat gtatcacctg cggctgttgt gtcaacggca
    22561 tccactttaa aagcagggac gataccgtgt tctttttctg tatgatagaa agcgccggca
    22621 ctgcctagtg taatgattac tgttccaata cctaattcat ggagtttttc ggctgctgcg
    22681 accaaactgt tatgatctgt cactctgatt cctgtaatga tttctgtttc tgtttcattt
    22741 ggtataatga tatctgtctt ttcaagtagt tcagtaggaa tcgtttcacg agctggtgca
    22801 ggattcagga tcgttgtttt tcctgcgtct tttgcaatag aaaaagctag gatcgtcgca
    22861 tctaagctac tttcaaactg agcaataata aagtcactgt ttgtaatcag ttgcttgttt
    22921 tttaggactt cttgttcatg gaaagcatta tttgcccctg catggatcaa gatactattt
    22981 tctcctgctg cgtctaccat aatcattgct tgtccggttg tggcgccttg gatttcagct
    23041 atcccagatg tatcgatgtt ttcttttgtc agtagatcta ataactgttg tccttcgctg
    23101 tcagccccaa cgcctccaat aaaagatgtc cgtgcgcctg aacgtttggc tgccactgct
    23161 tggttggcac ctttacctcc gccagcgtga aaaatctcat gcgcatgcat cgtttcgcct
    23221 ggcttgggca tgttggttaa acgaagggta gtgtccatat taatacttcc aattactgta
    23281 accgtgttca ttttacaaac ctccaactat ctcatttatt ttgatgggaa aattattttt
    23341 taaaacgttt tagtaaaacg ttttaatagt tcgataaaaa tatagcacac cttgaaagct
    23401 atttcaaggg tgcagttgaa aatctttttt ctaatcgtac aggtaatctt ttttcttccc
    23461 attctttttc tgggaattgg atccgatcta acaataactt ggcagatgtt tgtcccaatt
    23521 cgaagattgg ttgtgcgatc gttgtcagtt tcggcttgat atactcgcac atatctatat
    23581 tatcatagcc aataatgctg tagtcttcag ggatgctttt tccagcttcc tctaatccgc
    23641 gatataatcc aaaagctaat tcatcattta aagcaaacac accagttgca gcagaatcaa
    23701 gaagttctgc tgtgatctgg tagccacctt gttttgagaa ctgtgtaggt aagaggatca
    23761 gttggtcgtg agggatttgg taaacatcaa gagcagattt aaacccctca atccgagcgt
    23821 ggacattttc cggtggattt tcaggataga caagggcgat cgtttgatgt cctagactaa
    23881 gcaagtgcat gcccgctagg taaccaccgc gaaaatcgtc tgttcgtacg gcatcactaa
    23941 atccttcaga ttttttctga tccaaaacaa tgaagggccg gccttgtttt ttcaaatttt
    24001 cgttgattgc atctgtagag acagcagaag tcgcgataat gaatccatcc acacctcgac
    24061 gtgtgagctc ggctagatat tcgatttctt tttgatgatc agaatcagca ttacataata
    24121 tcgtgacgaa attctgctta tacaagatat cctcaattcc tcgcatcagt gtactgaaga
    24181 atggattcgt gatatcagga acgagtacac caattgtttt cgtttccttg gtaatcattt
    24241 gtctagcaaa atagtcaggc tgatagccta attcttcttt ggcagctaaa actttctgaa
    24301 cagttttcgg actgaatttc tccgtatttc catttaatat caaggagaca gttgtgattg
    24361 aaacgccgga aagtttagcg acttctttga ttgtgatctt ttttttcacc atgttcatac
    24421 tcctatctaa atcctgtggt tattttccca tgaaatcaaa aagaagtaaa tgaaaatcac
    24481 gtgtgccagt acttatttgc tttgttggat agcttcgatt atttgtttcg ttgagcgtac
    24541 tcgtgcagtt ttcggaaata tcttttttat gaacaattga tgggattctt tgtcacgatc
    24601 actgcaagca tcttcagcaa gcacgatatg gtacccgtgt tgaaaggcat ctaatgcagt
    24661 agcatataca ccgtttgttg tcgctacacc gcttaaaatg atagtttcga ttccacgccg
    24721 gcgaagttga agatcaagag aagtaccaaa aaaagcactt ggattgtatt ttgtgatagt
    24781 caacatatta tctgtatctt tcaaagaatc cttcaaaagt aaatcagtga attctggagg
    24841 tacagaagca gtcattccac ccatatcagt ttgttgactc aaataaggga aagtggagat
    24901 gtctacgttc actaaaactt tcaaggcagc ggtacgtttc aatgttctta ctaacaaatc
    24961 attgtttgtc agaatagatg ctgcagtgtg tggagagagt gtaccgttat ttacgatctc
    25021 tttttgcatg tcgatcgcga caaaagcagt ttttgataaa tcaaaaatca tattttcctt
    25081 taaatcattc atgatgttcc ttctttcaaa taacaatgtt ttttatgtaa gtaaatcata
    25141 aatcgtttat ttgtcaacta aattaaagga ttcctttctt tagtctagag aaattcagtg
    25201 gtaaaataaa taagtgtgag aaaacgcatt cgatgcatag atatagcgag aaatatcgtg
    25261 aaaatggagg aatcagagtg aagtatgtcg aagtaaaaaa tgaatacaaa cgatacaaaa
    25321 tgggagaaac gaccattaca gcaaatgacg atatttcttt ttcaattgaa aaaggcgaat
    25381 tagtgattat acttggtcca agtggagcag gaaagtcaac gattcttaat attttaggtg
    25441 ggatggatac acctgatgaa ggtcagatca ttattgatga cacagatatt gcgcaatttt
    25501 cagataaaca gcttactgct taccgacgaa cagatgtagg gttcgttttc caattctaca
    25561 atctcgttcc taatctaaca gcaaaagaaa atgtggaatt ggcaacggaa gtttctcctg
    25621 atgcgcttga tcctgtagag gtattgaggc aagtgggcct agctcatcga ttgaataact
    25681 ttccgtccca attatcagga ggcgaacagc agagggtttc aattgctcgt gcactagcta
    25741 aaaatcccaa acttctttta tgtgatgaac cgacaggcgc gctggatttt gaaacgggca
    25801 agcaagtatt gaaattattg caaaacgcta gcagacaaca cggcaatacc gttttgatca
    25861 ttacacataa ttctgcttta gcgcccatcg ctgatagagt cattcacatt aatgatgcta
    25921 aagtaaggtc tgttgaatta aatgatcatc cttcatccat tgatgagatt gtatggtagg
    25981 gggattttat gaaaacaaga acatatctta aagcaggttt tcgcgaaatt cggcaatcta
    26041 aaggacgatt cattgcaatc atccttattg tcatgctagg aacactttta tttgtaggtg
    26101 tcaaaactgc tggacctgtc atgcaaaaaa cgatggatca ctatgttggt acagcaggac
    26161 tttctgattt gcaaatcgtt tctactggtg gcttgacaca aaaagatatt gctcaagcag
    26221 aaaagatccc agatgcaaaa gtagaaacag gaaaacagat ctattattcc aatactggca
    26281 aaaacgaagt gattcaaata ttttcttata acaaaaataa caagcagaac aagctacaat
    26341 taactgatgg aaaactcccg gaaaaaccaa accagcttgt attggatgaa aaagcggaag
    26401 atgaagggta taaaatcggt gatatttatc agattgattc agatgaattg aaagaaaaag
    26461 agtataccat cacgggcttt gtccgttctc cattgttcat caacaatttg gagagaggat
    26521 acgcgaacgt cggaaatggt agcgtcgatt attttgtcta tttacctgaa tccgttttta
    26581 aaacagatat ttattctgtc ttatatcttg attttacaaa cgtaaagaat ttgaatacgt
    26641 acagcaaagc ttacaaagat aaaatggagc aaaatcaaga aaaggcagaa aagtatttac
    26701 aagatcgacc cgaagaacgc ttagaagagc tgaaaaaatc ggccaacgaa gaattagaac
    26761 cggcacagca aaaaatagcg gatggcaaag cacaaacaac gcaagcaaga acacggttag
    26821 aagatgctaa aaaacagtta gcgcaacagt cagcaactat ccagcagctt cctgaagcgc
    26881 aacgaacaat cgctgttcaa acattatctc aacaagaagc tcaacttgct gaacaagaaa
    26941 ggcaattagt tgagaaagaa cgtgaactgg cctctgcaga ggaagaattg aatcgaaatg
    27001 cagaaaaaat tgaaaattta actaaaccat catacctata caatgaacgt tcagaaaatg
    27061 tcggttttca agaatttggt gatctgtctg atcgtatcgc tgcgattgcc aatgtttttc
    27121 ctgttttctt tttctttatt gccgcattga tcacctttac gacaatgaca cgtatggtag
    27181 aagaaaaccg cagggaaatc gggacattaa aggcgttagg ttatacgaaa gttgaaatcg
    27241 caaaaaaata tgcaatctat gcaagtctag cctcagggat aggtattgtt ttaggaacga
    27301 tattaggaac gaatcttttg ccaaggatca tttttgagct gtctaacgag cggtatgata
    27361 ttgggagtgc gcttattttt tatgactggc cgccgatttt acaagcagct gccgcattct
    27421 tcattgccgc atttggcgct gcaatgatcg ttttattcaa agatttaaga gaacggcctg
    27481 ctgcattgct tcaaccgaaa gcaccgaagc cagggaaacg catttttctt gaatacatca
    27541 ctccgctgtg gtcacgcctt agctttaatc aaaaaatcag ttaccgaaat ctcttccgat
    27601 ataagtcaag aatgcttatg gcgattatcg gaattgctgg atgtgccggg ctgatggttg
    27661 caggcgtggg attgaaagat tctctaagtt ctgtttcagc caaacaattt ggaccgatca
    27721 ttgactatca agcaatcgtt acattggatt catccacgga agatagtcgt caaaaagcag
    27781 cggatatgct gcagaagcaa gaaaaagtaa atgagatcct agctattcat actcaaaccg
    27841 tagaattgag aaaaaaagga catgcttcgc aaagtctgac agtgatcgta ccaagtaaaa
    27901 cagctgattt ggatcgtttt atccatttaa ctgatcttga agagaaccca cgctccttgc
    27961 ctaaaacagg aatcgctatt acgcaaaaag tagcagaact atttgactta tctacaggag
    28021 atcatatttc gctttatgat gaagatcaaa aagaatggaa agtcgaagta aaagaaatcg
    28081 tgaaaaacta tctaggtagc tttatttata tgtcccctga atattatgct tcagttactg
    28141 atcaatcgat gatggataat gcatttttgc tgcgtacaga taagatgaca acaaaagaag
    28201 aagaaatgct ttccgagaaa ctgcttgcca cagacgctgt gacaaatacg tctttcatgt
    28261 ccaaacaaat cgaaacgcaa gaagaatcaa tggcgaactt agactcggtt gtcttaatct
    28321 ttgtcgtatt atcaggactg ctggctttta tcgtcttgta taatctaact aatatcaata
    28381 tttccgagcg tgtacgcgaa ttatctacaa taaaagtcct tggatttttt gataaagaag
    28441 tcacgatgta cattgttcga gaaaatatca tctttactct tttaggaatc atcggaggat
    28501 ttggtgtagg atatatattg actgacttca tcttgcaaca agcgtctatg gagaatgtca
    28561 tctttccatt agtgatcaca tggtgggctt atgtcttatc agcaggatta actatcgtat
    28621 ttacggttat tgtgatgatt gttacccatt tcaaattaaa gcatatcgat atgattgatg
    28681 cgttgaaatc gaatgaataa aaacagaggt tgtgaaatca gcggtttgac ctgagtatta
    28741 gagaaacaaa cggcaaaata gtttttcata ttttgccgtt ttttgagcta ataccgaagg
    28801 acagctgatt gaacaccatg aatcagaggt tgtaaaaaag ccgtcctgca tcgagcaaaa
    28861 agaatactca aataaaatta gctcctcatg ttttacattt gagaagactg attgccgtag
    28921 ataaaagctt ttgaacacca tttatagaag aaacaagaat attttgctat tttaaacaag
    28981 aggatagaca aatacatgtc tgatctcttg ttttttattg ggcaacagac ctcttagaat
    29041 gtataaagat aaattttaca aacgctttcg gttcagttat aataataaat gataacttgt
    29101 atatataagt tgtggtaggt aataaatcag ttgaatggag gtaaatatcc atgacattta
    29161 aagacaaagt catttaccaa atctatccca aatcttacaa agacagtaat ggtgatggaa
    29221 ttggagattt aaaaggaatc aaagaaaaac tcccgtatct gaatgatcta ggcatcgaca
    29281 tgatttggct aaaccctatt tatccaagcc ctcaaaaaga caacggttat gatatctctg
    29341 attacacagc catcgatcct atctttggta cgatggagga attcgaacag ctaatagccg
    29401 aagcaaagaa ataccggata gaaatcatgc tcgatatggt gctgaatcac gtttcgacag
    29461 aacatgaatg gttcaaaaaa gcactagcag gggaccagca ttatcaggac ttcttcattt
    29521 taagagaaga gccaacggat tgggtatcaa aatttggtgg aagtgcttgg tcgccatttg
    29581 gagatacggg gaaatattat ttgcacttat atgatcggac acaagctgac ttgaactggc
    29641 ggaaccaagc agtaagagag gaactattca aagttgttcg tttttggatg gaaaaagggg
    29701 taaaaggatt ccgtttcgat gttatcaatg ttatcggtaa agacgaacaa ttgaaaaata
    29761 ataaagaaaa tgatggcaaa cctgaatata cggacaaacc aatcacccat acgtatctaa
    29821 aagaattgaa ccaagcaacg tttgggacgg attccactat catcacagtc ggtgaaatga
    29881 gctctacgac gatcgaaaat tgtatcttgt atacagaacc tagtagagaa gaattatcaa
    29941 tggtctttaa tttccatcat ttgaaagtcg attatgaaaa tgggaataaa tggtctactc
    30001 ctccatttga ttttgcagaa ttaaagcgac tatttcatac atggggagaa aagatgagtg
    30061 agggtaacgg ctggaatgct ctatttttta ataatcatga tcagccacgt gcgctcaatc
    30121 gatttgtagc tatcgatcag tatcgaaaac aaggagcaga gatgctagca accatgatcc
    30181 atttgaaccg tgggacgccc tatatttata tgggagaaga aatcggcatg atcgacccag
    30241 actattcatc gatttccgac tatattgacg tagaaagttt gaatgcttat aagttactcc
    30301 ttgaacaagg attcacagaa aaagaagcgt ttcgtcggat caaagcaaaa tctcgcgata
    30361 actctcgaac acccatgcaa tggtcagaca cagaacaagc aggatttact acagggaaac
    30421 cttggttaaa agttggaggc aagctagaag agattaatgt agaaaaagaa ctatcttctg
    30481 aagattctat tttgtcctat tacaagaaat tgattcggct gagaaaaaca tatccaatcg
    30541 ttgcacaagg agattatcat gcttatgaag caaatcatcc ggaagtttat ggctttcttc
    30601 gccaatttga aggacagcag ctgcttgtat taacaaattt ctatgataaa gatacaagga
    30661 tcgccatccc agaaggattc acagaagcca atattcttat cgataattat tccgatactg
    30721 ttcttcatca aagtctggag ctaaaaccat accaggcaat tgctttgctg aaaaattaaa
    30781 acaaaaacac aaaacgtcgt tcctacgagc tattctcgta agatcgacgt tttgtgtttt
    30841 atttaaaata atcttcttca gaaaatgaag agttttcata ctctccttta tcgccataaa
    30901 atttattgta gcgttgtttg aatttacgga aaataaaagt aacgagtaat ttgactaacg
    30961 tatagatagg aataccaaaa ataacgccca caattcccat tagatcaccc atgacaagta
    31021 aaacgattaa gatcgtaatc ggatggatct gtaaacgatc acccattact cgaggaatca
    31081 ccaagtcacc gtggagaagt tgtacggcaa accagacaat gacaaatttg acagccataa
    31141 aagtagagtc ttggaatgca atgatcaaac ctggaaagaa ggcgataaac ggcccaatat
    31201 atggaatgat acataacaac ccagcagaaa tagctaaaac actagcaaat tctaatccga
    31261 tcagtaagaa acaaacccag tagactgctc caaggataaa tgaagcaatg atttgcccct
    31321 ttagaaatga acccaattgt tgattagcaa tctttgtcaa atttttaaaa tctgtacgaa
    31381 atgctggtgg gacaatcgct aaaactgact gataaaattt ttgaggatct ctcaaaagga
    31441 aaaaagcgac aattggacca gtaaataaag taataaaaat agctgtaaca gttgaaaaaa
    31501 tgttgctgat ccgttgtgcg ccactttccc aataatttcc aataaatcca gctatatcag
    31561 acgtcacgtc gtcaatagaa gcgaaaaatt gattaaatga atcggcaaaa ggtgttttct
    31621 ccaaaaattg tcggagactc tcttgaaaat cttgaaacaa atctggcaaa ctactgatca
    31681 atgtctttgc ctgttcttca agaaaaggaa taagccatat gcttccaagc cccaataaaa
    31741 ataaaataat cacataaacg ataatgactg acaagttcct cgaaaggtgc ttgctcaaac
    31801 gtttgatgac tggttccaaa agataataca agatcagtgc aaacaataat gggggcaaga
    31861 tagtagcgat aatgattccg acaggcttga atataaacga gatctgatgg aaaaaataaa
    31921 tgatcagcgc aagtaagata actattccta aaacatagta agaggttttt cctcctaaaa
    31981 agcggatcaa acgattcgtt gtatctgttt cttttttcac tctttttcct cctttatcta
    32041 taaattcatt atagacaaaa gcgggtataa aaggaaaatg atagaggttg attgcaaaaa
    32101 tatcgtcaat cattcatata aactttctca tcaattaaaa taagaaaacg cttgacagtt
    32161 cttttaacca tatgataaat taaatcctgt tgctaaaacg taactattac ggtttgcgaa
    32221 aagcgaatcg gaacattcaa atgaaagaag atgagaagag gagtgtcata aatgaaacga
    32281 ttgataggta tattggccat gctggtttta gcaggtatgt taactgcttg cggggcaagt
    32341 gggaaagccg aggacagtaa agaaaaattg agcgtgatga cgacctttta tcccatgtat
    32401 gattttacaa aagcaatcgt cggggatgaa ggagaagtag agttattgat tccagcaggg
    32461 actgattccc acgattatga accttctgct aaagacatgg caaagatcca agatacagat
    32521 atctttgttt ataatgatga aaacatggaa acatgggtcc ccgcaatcca aaaaacatta
    32581 caagaaggca atgtccatac gataaaagcg acagaaggta tgcttttact tccgggaagt
    32641 gaagaaggac atgatcatga ccatgaacac ggggaagaag gccatacaca tgagctagac
    32701 ccacatgtat ggctagctcc aagtttagca atcaagcagg tagcaaacat acgtgatcag
    32761 ctgattgaag cttacccaga aaaacaggaa gtatggacaa aaaatgcagc agcctatacc
    32821 gagaaacttc aagcactcca tcagctttat caagaaacct ttaagcaagc aaaacaaaga
    32881 tcttttgtca cgcaacatgc tgcattcaac tatctagcgt tagaatatgg attgaatcaa
    32941 gtatcgattg ctggtctttc ttctagtgaa gaaccgtctg cggcgagaat tgctgaactg
    33001 aaacactttg ttaaagaaca tggaatcaac tatatttatt ttgaagaaaa cgccaaagat
    33061 agtattgcca gaacgttagc aaacgaagca ggtgtatctt tagaagtatt gaatcctttg
    33121 gaaggattga cgaatgaaca aatagaaaat ggagaaaact atctttctat catggaagca
    33181 aacctcgaag cgttgaaaaa aacgacagag acagaaaacc cgctagaaga tactttgacc
    33241 cccaaaaaag aaaagagcgt ctacaacggt tattttgaag acgaggatgt acaagatcgc
    33301 tctctttcag attgggcagg aacttggcaa acagtagata cgtatgtaga agatgggact
    33361 tttgatcctg tttttgaata taaagaaaaa ttgaatcagg ataagacagc acaagaatat
    33421 aaagaatatt atacgaaggg ctatcaaaca gatattgctt caatcaagat cagtgagaaa
    33481 gagatggcat ttacgtttaa gaatggaaca acgaaacgtt caaattatcg ttatgtaggg
    33541 aaagaaatct tgacctacga ggcaggaaac cgtggcgtaa ggtatctatt cgaagcagaa
    33601 gataaggaaa gtggcgcttt tagatatatt caattcagtg atcacacgat tgcaccggct
    33661 gactccgaac attttcattt atatgcagga aatgaaagtc aagaagtatt gctgaaagaa
    33721 atggagaatt ggccaacatt ttatccagat acaatggatg gacgtgaaat tgcacaggaa
    33781 atgcttttgc actgataggc aaaaaagaac cgaagggttt ggatcggctg gtcatttagt
    33841 cggtccaaac tattcggttc tttttatgaa atcaatttct ttaatccacg gatagaggct
    33901 gggatggtct cgccgtttgc caacgtgact tctctggttt ttttattaat cgaccgcaca
    33961 ttttgtgtat tgataacata ggatttatga caacggataa aattccgatg cattttctcg
    34021 atttctttga tcttctcata gaattgtaat tgggcatgag tcaaatggac ttccagttta
    34081 tgtggcgctc ctagcgaagt agaaaaatat tggatgtctt tgatcggtaa aaatttgatc
    34141 tcatcattaa actctatttt gaagatatct ttttggattt gggacatttg aaaatcttcg
    34201 aaaattttgc tcaggatatg ctcgacattt tcttttaaat cataaagatc ttctttgacg
    34261 atataatcag tcggttcgat gttgcttttt aagattttca tcgacatttc cgtatgggaa
    34321 gtcacataga ttatttttcc taaaggatct aattcacgga tcaatttacc gatcgctaca
    34381 ccatccattt cactgtcttc tagttcgata tctaaaaagt aaataccaaa agaagaagtt
    34441 ctgatcttgg ctgcattgag aagttcttcc ggatcagagg tagaaatatg gacataactg
    34501 tctaagtttt caatcatgat tctatttttg atcaccgttg ataaaacttc taattgtttt
    34561 ggatcgtctt cacaaatata aatgggaatc atatcattac tcctttattt tatttgtata
    34621 taaatcgttt gagaaaaacg ctggttttca attgatgttt caaggaaaag ataaggattg
    34681 gtctttgtca attcatccaa gataaacaag ccaaggcctt cgttttttgg atttgttttt
    34741 gttgagaacc ctttttgttt caactggttt aacggtaatg tatttgttgt tgtattgatc
    34801 acacgaatca tcacggaatg attcttcttg agtatggtca attcgatttt tttcttcttt
    34861 gcagcactag ctccttcaat cgcattgtcc aagataatac caagcatacg tacaaggttt
    34921 accgtatgtt tcgtatccag ttcgattggc tcaggaaaat gaagctggac gatcaaccct
    34981 ttttcctgtg ccatcatgag ttttaatgac agtaaactac ggatctcagg aactttcata
    35041 ttatccaaca tacttagatt ggccggaaaa aggttcatca tctggcgtgt aggtaatatc
    35101 gtttgatcat aataagaccg taatccttct aagtcgcctt ctgaaatata gctgtgaaga
    35161 gaaaaaagca gattttggta atcatgtttg aattctctca agtcttgata gtttttttct
    35221 agatctttga tataatcctg ttgcatcctg acttgttgct ctaataactg atcagctaat
    35281 ttcttattag agattttcca ataataaacc aagatgatca ggccaacaca cagatataga
    35341 atgaaaaaac tcgtattcaa agctaagaca tcaggtgttt caccagaaaa acgggtataa
    35401 aaaataatga tatagtatgt aaataatatc aatgtcccga tcgtaccgat catacggcgg
    35461 tccacagtgt gaaaccaagt ctctaacgat ttttttaaac aaaaagctaa taaagcagat
    35521 aaaaaagcgg ataagacaat gtgaaaatac tgcaatgaat ctcctggttc aataaccgaa
    35581 gatttcaaaa caaaaaaatc gccgatgctt gttaagtgat ctcctaaaag cgacgtcaat
    35641 aacccaaaag gaagcgcgta aaccaattgt tgacgcgaag tcgaaaagaa gaaaaaagta
    35701 agcatcagca ttcctatagc aattagagca gtaaaaatcc ctaaaatcga atagacccat
    35761 ccaatcaaaa tacaagaaat cagaatgact cccgcaaaca gatatttgta tcggatgtga
    35821 tggtcaatca ataagataca aattgtgtaa atcaaatatt gcaacgctat ggaccataaa
    35881 aacataaagt acagctccca ttgattttta ccaaattata gcacaattgg cgtattattt
    35941 ttttaatttt tcagggattt tgaattcaga actcatacca aagcagcagc cagatacaaa
    36001 aaagtcagat aagttagtta attgtttaat gattgttttt ttcatgaaag attcctccaa
    36061 aattcatcaa gtagtttgta ataggaagca acgtagctat ttctaaaaca atcccaagta
    36121 gcaaataggt agaaaagtaa gaagctgaga aaaataacgt aatgagagaa aaaattagaa
    36181 acatgctgat cgccttcttt aataatgggg gacgtttctc gggttcaatg ggattgattg
    36241 ctgtacctct tgatccatag cgaagcagta acaaaaagca gctgattaac aaaaagacca
    36301 tcattgggaa ggagatagtc aattgcagat gtttcatcac caagggaacg ccccataaat
    36361 aaagtagtgt ccacagcaga caaacgaatt cagagtcgga atgcaatcca taagcatatc
    36421 tgcggatgca gatataagaa agaaaggtga ttcctgtttc cgcccatgag cctgtcacca
    36481 gagaaaagac tagaacgacc gctagttttc caagattatt caataaaact tctaagacaa
    36541 attttacttg gacatacacc atatcttctt ccgatttgtc tccgctgaat aaggcttcta
    36601 ataatttgtt cacaacttta tctttcatct catacgtacc tccaccacaa attttatgct
    36661 aaaaaaaata aaaaacgtct tttgtttcat aaaaagtggt tttgacatca aaaccgtcat
    36721 gatctcctaa aaaaatcttc gttttccaag aaccttacca aaaaaagtga cagttttgat
    36781 acgaaaagta ctttttatga caagtttgtg ctttttcgag tgatttatgt taaaaaatta
    36841 ctgaccttat aaaaggtgat ggagggattc ttatggaaaa tgaacaattg tttgaagtcg
    36901 gcgaaacata tgcatttaaa tctgatttat ttgaatcacc aataaaagga aaaatagaaa
    36961 aaatctatga aaatagtgtg atgatacgtg tcatgaaatg cgcagtagaa gacaatgata
    37021 ccgctcataa tctgcactgg cagttagttg tacgcaaatc aagtacaata gaagaataaa
    37081 aacagataaa atgttgagag cataaagagg ttgtgagtct cccgtttagc tctgagttac
    37141 aaggaataat gttaaaaaac agcttttcat gttttatgaa tattgccttg tgaccgaggt
    37201 gttggcagga gaatactgtt tatttgaaaa aagggctgcg acaagacttt tgtcacagcc
    37261 tttattttca tgaataggag ttacttttgg ctacttttag agaagtagct tttaaggagc
    37321 agataaagtc aaggctttta tttctataaa tgaagtagta aaatgaagaa cagattttat
    37381 tacaggaagg atgccgataa ttgtgatcat tgttgcagga acgatcggtg cagggaaaag
    37441 cacactaact gaaatgcttg cccaagattt agaaacaaaa cctttttatg agaacgtgga
    37501 agataatgaa gtattgccgc tattttattc taatccagaa aagtatactt ttttattaca
    37561 gattttcttt ttgaacaaac gctttttggc aatcaaagat gctttcagcc atgatgacaa
    37621 cgtactagac cgctccatct acgaagatag tatgctgttt catttgaatg cagatttagg
    37681 gcgtgtttct gaagtagaag taaagcagta tgaaggtctt ttagaaacga tgctgaagga
    37741 gttagaagaa atttcgccac aaaagaaacc agatctactg gtttatattc gtgtatcctt
    37801 tgaaactatg ttagcgagaa tcaaaaaaag aggccgggaa tatgaacagc tagaacaaga
    37861 tcctgaactc tattcctact ataaagaact caatcgacga tacgaagagt ggtatgaaca
    37921 gtttgatata tgtcccaaaa tagtgatcga tggggacaaa tatgatttcg tagccgatcc
    37981 ggcatgtgga gaacaaatcg tacaagaaat caagatgcgg gcaaagaaaa tgacagaaga
    38041 ggcagatgcc cagtcgatcc gctacactac aaataacccg aaagcaaagg gaatctttac
    38101 aactaaataa tataaaaact aagcaaattg ctcactcgca gatatttctc tgtgagcagc
    38161 atcttgctta gtttttttga ttcattgaga tcaaatagtg aaccgattcg tatgataaaa
    38221 ttgccataaa tgaaatgtct gagtgagggc catgatcaaa aataaaatgg aaacgatcaa
    38281 aatcgaatgg gcgccaaaca gatgatgaca gaaaacaagg acaatagctc ctgcgataaa
    38341 ggcaagaacg acacttagat agtggaaaaa tcgatcaaaa tctgcttttt ctttagaaat
    38401 acaataatca tataagttcg ttgctacccc ttttaaattt ccagttgtaa ataaattagt
    38461 gtaggcacgc ccgttgattt tatcaaaagc tacccactgg atcgatgcag taaaagcaat
    38521 caaaatcgtt actaatgcgg gatgctcatg gacgggtgtc aaactaacaa agaaaaaaat
    38581 cactgcttca taatatagaa caaacaagcg ccagaaatgt ttttcttttt ttctaaaata
    38641 atcaattaga tactttgtta gaagaatccc taagaaaaag aaaagggtag acaatacttt
    38701 tttaccaaca cttagccatt ctccgtcaaa tgcttggatg atcgatagaa tgatattgcc
    38761 agtttgagca gaggcaaaag caccatattg gatataagta taactgtcca tggctcctcc
    38821 gataaatgtc agcaaaagtc ctattgtacg cccctcgtga aaggaagtct gcattttttt
    38881 catcaccttt tatgaatatt tttgtcttcc ttatcttagc ataaacgatt gaaaaaaagg
    38941 aaaaattttc agaaaactaa aatcatatcg cttcccattt tcatttttag aaaggtctgt
    39001 tagtataaag aaaaaataga caacgaaact ttaaataacc aacttgaaat aaacaaattc
    39061 aaagaagaaa gatgggaagg aaatcaatca gttgaatgaa aaagaaattc tccgattaca
    39121 aaaagaattg aaacgctggg agaacaagta taaaaagctt caacaagtct ttcggctaca
    39181 agaagaaatc attgctacct atgaagcggc ttatggcgaa gcacccggac ttattgagga
    39241 agaagtacaa gtaaaagaaa gtgaaggaaa cagctctttc tttaaaatga tggagaaacg
    39301 ttcctataat cgcttcaatc aagaacaaaa agcagctatc acatatgata tgaagaaaca
    39361 tcttcgaatc atagcaggag ctgggagcgg caaaacgcaa acaatctgtg caaaagctgt
    39421 ttatctgatg acgcaaaaac aagtagacga agaacgcata ctaatgatca cttttacaag
    39481 gaacgctgca aatgaactaa aaaaacgagt agataatttc agccagagaa aaaccgctgt
    39541 ccatatcgga acattccata gtatcttttt taggatatac aatgaaattt gtcgaaaatt
    39601 tcctgaagta gcaatgcaag gaatacaagg agacttttca aaggatactg cacaaaaagt
    39661 gaatgctgtt ttgcaacagc tgatacgtaa atataatctt tatatatttg atcaatatgg
    39721 agaaaaaacg attgcttctc ggttagacta ttggcagaat atgaaccttt cagtagaaga
    39781 aatggttcag ttagttaaag aaaaatatga ttccattgat aagaatgcac gacagccgat
    39841 atcagaacga ctgtatggat tgctcacgga actccaagaa caaaaaagaa gccagcaatt
    39901 gctagagttc aatgatgtac tccaaaatct gaaaagcgct ttggagaatg aggagatcca
    39961 gcattatatc ggccaaaaat atgattatct attcattgat gaatttcaag atactaatcc
    40021 acttcaatgg gaaatcgtga aacggatgac aaaggaaaac aggatcaagt tgattgttgt
    40081 tggagatgat gatcagagta tctatggatt cagaggatca gagccagcat acatcaagaa
    40141 ctttgaaaaa gaatatccta ccaaaactct tttcttatta actaattatc gttctcgggc
    40201 agctattgtc caaggagcaa atagattgat cagctataat aaaaatgatc ggataccaaa
    40261 ggcgatgatt ccagcacaaa aagaagcagg tgttatggaa gcctatttgt tttctgatac
    40321 taaagcagaa agtcagtggc taatcaaaca aattcagaca tttattcaaa aaaatgggaa
    40381 atataaggaa tcaatcatat tataccgatc agcttcacaa acgcagcagc ttgtccaatc
    40441 tctgctgaaa acgaatattc cttttgtact ggaagcagat agtccatatg aaggtatctt
    40501 tggtatcaag tctttccaac atttctatca aaaaattgtt cattggcaaa cagcactgaa
    40561 tctgcaaaag aaaaatcagg cctaccagca attgctccgg cagctgatgg cagactgcta
    40621 cctgaaaaaa agtgaatgcg atcaatactt ttctcctaaa tatcaaaata tagccgttgc
    40681 agattatatt ttgtcgataa gccccaattt aaaatcgaaa tctgcagaaa tccgagagtt
    40741 tgaggaagcg ttgcgtaaaa cagaccagca tcaagaaatc tattcgcttg ttcaaagcta
    40801 tcttcatctg ccacgtatag ctaaggaaat cgaccagagt gatcaagaat ggatacgaga
    40861 agaagtaaag cagcatgcta ctttcagaag actccaagcc ttatcgcaag aaacccaagc
    40921 gacagctaaa gaattaaaaa cccgcttgat tgcttacaga caaggaaaac ttgatgcact
    40981 atgtatccag agcattcaca aaagtaaagg tcttagttac cgaaatgtct ttttgattgg
    41041 ttgtaacgaa ggaagtttgc cttataacgg agcgacagaa aagaaagtaa tcgactttaa
    41101 tgaaataaaa gcagaaccag taacgacgat agaagaagaa cgaagactat tttatgttgc
    41161 gatgactaga gcaagaaatc gtctgtatct atgtgtacct caaatgaaaa attcaagaaa
    41221 attgaaagta tcgagattta tcaaagaaac cgggagtcgt gtgaaaaaag gaaagtgata
    41281 gaagagaggg tgtgacagaa gtggtcagtt tcaagaaaga agaaggaatg tacgaaaatt
    41341 gcttctcaaa tttttgtgaa atttcagctt attttcgaag aagctatttc tggaacacag
    41401 tttattcgga aaagggaggt gtgacaagaa ttttgtcaca cctcccttat ttattttgaa
    41461 aatgagaata ctattatcaa aatagagaat atatagaaaa attctcatta ttcaaacgta
    41521 aataatcagt tattttgatt ttttttatat tttattagct aataattggt attttaaaag
    41581 aaattactgt agaattggat tataattaaa aaggagtgag aaaatgataa aagtatatac
    41641 ttcgcctagc tgtacttcct gtagaaaagc aagagcatgg ttgcaagcga atcaacttga
    41701 atttgaagag aagaatattt ttgccgaacc gttaacagaa agtgagatca aaaagatctt
    41761 ggcagcgact gaaggaggag ttcaagatat tatttccact cggtcgaaaa tttacgaaaa
    41821 gctgggcata gattttaacg aattaacttt aaagcaaatg atcgtcttat tcaaagaata
    41881 tccttcatta ttgagacgac ctattttgat tgatgaaaaa agaatactaa tcggatttaa
    41941 tgaagatgag atccggtcat ttgtccctcg tgaaattcga tgtgttgcca gaaatctgat
    42001 ctatttacaa aacaataaca tcttaaattc atagggaggg agccttcgtg gaagtgaaag
    42061 tctatggatc agcgcactgt gtaacaagtc tgaaggcgaa acaatggtta gaacaccatc
    42121 aattgcctta tcaatttgtt gacttagaag aacgcgatct aagtacagaa gaagcagaag
    42181 aattatgcag ttttgaagaa gtgcaagcag aacagctatt tgcgacttgg tctgaggctt
    42241 ttcacaacat tgcgattgac ctttcctgtg taggaaaaaa acaactgatt tttttatgca
    42301 cacaacatac caatctcctc agacgtcctt tgattattat cgatgaagtt ctatttattg
    42361 gatacaatga gcagttgatg gaacagctta tcttaaataa atagacttta actgttttaa
    42421 tcaagtcact gagcatttca tacagttgta acagtcgaac acttagcatc aagcgtgtta
    42481 tgtgttcttt ttttgacttt atttttctac tatagagaca tgctcaattt atgtggtcaa
    42541 aattcaaatc taggtgggga tatagagtta aaaatatatt gacatgtgta aatttacatg
    42601 atatattcat ttctgttgtt ttaagtagca aagcaataaa atagtatttt aaaaaacata
    42661 taaaatagtt gttgacatgt tagaaatgac atgataagat aactgagttg ttaaaaaaga
    42721 accacttgtg aagcttgatt ctatcgaaaa aaacttcaaa aaagttcttg acaaataaca
    42781 aatgataagt tatgataaat gagttgctgt aagatttcga aaaacgaaat caaaaaaact
    42841 tttaaaaaag agttgacaat tacttgtctc tctgatatga tataaaagtt gctacggcag
    42901 ccaaaaacga atcggggaac actcctgatg aagtagacct ttgaaaactg aacaaagtaa
    42961 gacaaaccaa atgtgtaggg cgtcttgatt caattcaaga caacaaacat tttttaacaa
    43021 gcaagcaata tgctagcaaa caaattgagc ttaacaatcg caagattgtt cgaacttttt
    43081 atgagagttt gatcctggct caggacgaac gctggcggcg
//
LOCUS       DACCCS010000019        38228 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.019, whole genome shotgun sequence.
ACCESSION   DACCCS010000019 DACCCS010000000
VERSION     DACCCS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.019"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(451..1761)
                     /locus_tag="IVK82_001075"
     CDS             complement(451..1761)
                     /locus_tag="IVK82_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP6392151.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IVK82_001076"
     CDS             complement(1773..3191)
                     /locus_tag="IVK82_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6392152.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="IVK82_001077"
     CDS             complement(3431..4999)
                     /locus_tag="IVK82_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP6392153.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="IVK82_001078"
     CDS             complement(5003..5413)
                     /locus_tag="IVK82_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP6392154.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="IVK82_001079"
     CDS             complement(5410..6333)
                     /locus_tag="IVK82_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP6392155.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="IVK82_001080"
     CDS             complement(6330..7208)
                     /locus_tag="IVK82_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP6392156.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="IVK82_001081"
     CDS             complement(7195..8532)
                     /locus_tag="IVK82_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP6392157.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="IVK82_001082"
     CDS             complement(8535..9638)
                     /locus_tag="IVK82_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP6392158.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="IVK82_001083"
     CDS             complement(9712..11220)
                     /locus_tag="IVK82_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6392159.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="IVK82_001084"
     CDS             complement(11186..11878)
                     /locus_tag="IVK82_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6392160.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="IVK82_001085"
     CDS             complement(11900..12874)
                     /locus_tag="IVK82_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6392161.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="IVK82_001086"
     CDS             13174..14046
                     /locus_tag="IVK82_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6392162.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="IVK82_001087"
     CDS             complement(14504..16702)
                     /locus_tag="IVK82_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6392163.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="IVK82_001088"
     CDS             16934..17926
                     /locus_tag="IVK82_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6392164.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="IVK82_001089"
     CDS             17986..19407
                     /locus_tag="IVK82_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP6392165.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="IVK82_001090"
     CDS             19400..20899
                     /locus_tag="IVK82_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP6392166.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="IVK82_001091"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="IVK82_001091"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP6392167.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="IVK82_001092"
     CDS             22491..23174
                     /locus_tag="IVK82_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP6392168.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24005
                     /locus_tag="IVK82_001093"
                     /pseudo
     CDS             23176..24005
                     /locus_tag="IVK82_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            24016..25770
                     /locus_tag="IVK82_001094"
     CDS             24016..25770
                     /locus_tag="IVK82_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6392169.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26046..26387)
                     /locus_tag="IVK82_001095"
     CDS             complement(26046..26387)
                     /locus_tag="IVK82_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP6392170.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26455..28626)
                     /locus_tag="IVK82_001096"
     CDS             complement(26455..28626)
                     /locus_tag="IVK82_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP6392171.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28837..29694
                     /locus_tag="IVK82_001097"
     CDS             28837..29694
                     /locus_tag="IVK82_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP6392172.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29765..30622)
                     /locus_tag="IVK82_001098"
     CDS             complement(29765..30622)
                     /locus_tag="IVK82_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6392173.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30607..33309)
                     /locus_tag="IVK82_001099"
     CDS             complement(30607..33309)
                     /locus_tag="IVK82_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP6392174.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33322..34611)
                     /locus_tag="IVK82_001100"
     CDS             complement(33322..34611)
                     /locus_tag="IVK82_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP6392175.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34761..35807
                     /locus_tag="IVK82_001101"
     CDS             34761..35807
                     /locus_tag="IVK82_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6392176.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35809..37962
                     /locus_tag="IVK82_001102"
     CDS             35809..37962
                     /locus_tag="IVK82_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP6392177.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11281 a         7859 c         6800 g        12288 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgcctatcaa gccaagatga ttgctacgga aaccggtagt gtctctgtcg
    23521 gttacacaaa agaaaatttt actgaagaag cttatcagat cgccagaaat tctatcattg
    23581 aattagtcgc tcatgcagaa aattttggcg taactgttgc tgttgaagca gggatcaatc
    23641 atccaatcta taactatcag ttagctaaac gtttaatcga tgaagtagct tcaccaaatt
    23701 taaaaatcat tttagattgt gcgaatttga tgcataaaga aaatcatgct gatcaggaaa
    23761 aagtgatccg aggtgccttg gacaatttac atggttatat tacagcgctt catctcaaag
    23821 actatatcat ggaaaatgga aagatccgca tggtacctgt tggcaagggc tgtttagatt
    23881 tccgcccggt actgcattat atcaaatatg agcgtcctct aatgtacgcc actcttgaag
    23941 ctactcctga gatttatgtt cctgaagcaa tagaacactt gcagtttatt taccaagatt
    24001 gttaaggaga agattatgga ggaacgaata cagaaaagtt atcaaaaata tcatgcaaat
    24061 gcttttcaga cattgctgca tctttatcag ggaaattatt ggcgttttgc catgtcagga
    24121 ctctttttta ttatcaaaca tcttccatct tggttaatgc cgatcgctat tgccaatgta
    24181 atcaatgcag cttcatcgaa agattcttct cagttgcagg ccatttattt caacgctttt
    24241 ttgatggctt gtctgattgg tcaaaatata ttgacgaatt attttcatgt aaaataccat
    24301 agcacttcga tccgtcaagt agaagctgga ctaagagcga cccttatcaa gaaaatccaa
    24361 gaactatcta ttccttatca aaaagaattg caatctggac gaatccagtc aaagatcatc
    24421 cgtgatgtag aagctatcca aacgttgtcc tctcaagtat ttgtcagcgg attaaatatc
    24481 gctgtcaatt tattcgtagc tttaggtatc acgctttata aaagtcctgt tgttttcatg
    24541 tttttcttat tgactgttcc tctcgccgca ttcgttactc tctttttccg taaaaggatc
    24601 agacagtcca actcagactt caggcaagaa atggaagaga cttctgcaaa agtgatcgaa
    24661 gtgatcgaat tagcaccgat tgctcgagct catgggttag aaaaacacga aacaactcgc
    24721 ttaaataagc attttactca aatctatgaa aaaggattcc acttagatat cctgcaatct
    24781 gttttcggat cagtcagctg ggctagtttt cagatatttc agctgatttg tttgattttc
    24841 actgggatat tagctttaca agggcgcatc caacccggcg atgtcgtgat gtatcagacg
    24901 tatttcacga cgatcgtcaa ttccgtttct ggaatgatca cgctgattcc tacgatttca
    24961 aaaggcatgg aatctatcac ttctattggc gaactgttga ttgctggcga tgtagaaact
    25021 tatcacggta agaaaaaaat ccaacatttg gatggaaatt ttcgttttga ggatgtttct
    25081 tttctttatc cggactcact caaaccgatt atcgatcatt tttctctaga ggtgaaagct
    25141 ggagaaacaa ttgcttttgt tgggccatct ggctctggaa aatctacact ccttaactta
    25201 ttgattggat ttatccagcc gacttcagga aaaatttttc tagatgatca gcctttggat
    25261 gaactggata tgcgaagtgt gcgaaattat ttagccgttg ttcctcaaac cactttgttg
    25321 ttctccgctt cgatcaaaga gaacatcact tatggcttga aaaacgtatc aaaagaacgt
    25381 ttagatgaag tcatcgaagc tgcgcaactt tcttcactga ttgaccagct tcctgatggt
    25441 ctggatacct tagtaggcga acatggcaac aagctgtctg gtggccaaaa gcaacgtatc
    25501 tccattgcac gagcgttgat acgtcagcca aaaatcattt tgctagatga agcaacctca
    25561 gctttagata accagtcaga gaaaaaaatc cagcaagccc tgaattacct gacagaaaca
    25621 cccactactt ttattgtggc tcatcggctt tcaacgatca aagaggcaga taaaatcgtt
    25681 gtgatcgatg aaggaaaaat cgttgaaata ggtacttacg aaaaactgtt agctcaaaaa
    25741 ggctatttct accgcttgaa aaatgaatga caaaaaatag gagctatgac aagacttttg
    25801 tcgtagctcc tatttctgaa taaacggtgt tctcctgcca actcctcggt cacaagtcac
    25861 aatagtcatc aaacatgaga agctgttttt tgaaattgtt ccttgtaact cagagttaaa
    25921 cggtaggctc acgacctctt ggtaaatggt gttcaagaga cagcctatcg gcaataagaa
    25981 gcaattttgt catcgctcaa ggccgaacgt ctctttcaca ccctctgttg tttcataaaa
    26041 tagttttatt cgctgtaaag gtcgcgaact ggtgtcatga tgatacggtt caagtcattg
    26101 atgattgtac tgaagccttg ctctttttgc atcaagtcat tgattacttc ttctgattgg
    26161 attttcacag ccatttcttg tgctttcatt gcatcttcgt cgctgaactc ttgtccactc
    26221 atttgttttt cttgtaattc gatttgcatc gcttggaagt ctttgaacag tttgtgtgct
    26281 tcttcgtttg cttgtacttt ctcgtacgct tctttcaacg ctttgaattc tggtaattca
    26341 cggatctcac gttcgatttg attcgcgcta tcgtagatgt ttgacatgtt atgccctctt
    26401 ttcttttagt agttactctc tattgtaaca tggggttatt cgttttcaaa ggggctactc
    26461 tccttgatat ttccgccaga ggtctttagc cgcttcacca attccctgaa cgccttcttt
    26521 caccatatcg ccgaagtttt tcagcccttc ttttgcttgt tcaccatatt gattcaagtt
    26581 atcttgccaa tttccagact ctccattaga atttcctgtg tttccaactt gatctgcagc
    26641 taccacttgt cctcctgtgg cataggcatc tgctacacta aatcctgttt ttgaagtata
    26701 cggaaggatg ccttctgccg ctgatttgaa gactttcccg acaacatttc cacttgtccc
    26761 ttccaaatag tgcagcttgc tggattcttc gtaacccatc catgtcgaaa tcactacgtc
    26821 tggcgtgtaa ccgatcatcc attggtcatt tgcttttgtg gcatcgaaat tagtttctgt
    26881 ggttcctgtt ttccctgcta tcgtataacc tgcaggattc gcagcagccg ctgttccatt
    26941 tgaaaatgta ccaagaagca tgctggtcat ttcatcggct gtttcctttg atatgacctg
    27001 ttttggtttt ttgtttgtgt tatcgacgat caccgctcca gtcgaatcaa cgattttcgt
    27061 gataagatgc ggcgtataca tctgaccatc attggcaaat gcactgtaag cacccgccat
    27121 gatcaatgga gatactcctt tttccagtcc accaagggct aagccataat acttatcaga
    27181 ttttgctaaa ggcaggccga attcttctgc tttatcatat cctttttgta aaccgatctc
    27241 atgcaaaagc catacagtag gcaaattcaa actttgtgca acagcttgat acatcggtac
    27301 ttctccttgg tatgtcccgt caaaattatg cgcatcgtaa taactttgcg gttcgtcttt
    27361 caagatactg gaaggcttgt atccagcttc cagtgctggt gtatatacgc ttaatggttt
    27421 gatcgttgat cctggtgaac gacgcatctg tgtagcaaag ctgaatcctc taaagacatg
    27481 atctcctctt cgtcctacta gtgcttgtac accacctgta ttcggatcta aagctactga
    27541 agcagattgg accatggcac catctgaagc atttgctggg aataatgcac tgttttgata
    27601 agtagcttct aattgctttt gataatcctg atctaaagct gtatagattt tatagccttt
    27661 attcatgaca tcttcttcat caagaccata ctcgttaact gcttcatcga tcactgcatc
    27721 aaaataataa ggataacgat aaccagaatc tgaattatcg tatgtgtcat taagatacgc
    27781 gctgatatct gtctggcttt ctgcttgttc ttgttctttt gtgatttttc cgttttcttc
    27841 cattacactt agtactgtgt ttttccgatc attcgcattt tttgggtgat cgatcggatt
    27901 ataaatacca ggacctttca gcattcctgc caaagtagca gcctcagaca gcgtaacatt
    27961 cgttgcatct actccaaaat actttctgga agcgtcttgg acaccccata ccccgttgcc
    28021 aaaataggca ttgttaagat acatggtcaa gatttcttct ttgctgtact tcttttcgat
    28081 ctcgattgcc aaaaataatt ccttcgcttt tcgatcaaac gtttggtcta atgtcagata
    28141 cgcatttttg gcaagctgct gagtgatcgt acttcctccg ccccctgatg ttccgccaga
    28201 caagaccatt cggactgctg cacgggcaat ccctttgata tcaaacccgt gatgctcgta
    28261 aaaattccta tcttctgtcg agattactgc atcttggata tagggagaaa tcgcgtttaa
    28321 ttctacatat gttccttttt gggcgtacag tgtgccagct tcttcgtttt tcttatcata
    28381 aatgactgtc gattggctta atcctgattt taacgtttcg acatttgctt gttttgctaa
    28441 aataaagagg tagatacttg tcactaagac acagatcaaa ccaatcagca aaatcaattt
    28501 gttcacatga tattttttcc agatgcgctt tcgccatcga tggaattgtc cgagatatgg
    28561 tctgatccaa cgccaaaaac tgatcaaagc agcttttatc ttttctaaaa tgctttgaaa
    28621 atccatttgc ttcttctcct gtctatttga ttcaatcgtc ttattttacc atagtgaaag
    28681 agtgttttct tcctgcttaa gttgtagttc aatatgtttt tttacgttag tgcaagtaga
    28741 gtaacataag aatgaacaga aaaatgtaag ttttttctta aatatgtttt aaaaatggta
    28801 caattagtct gcgattgcac tgaaaggagt acacttgtga aaatcacgat cactttacct
    28861 atgaaccatc ctgaaacaac aattcgcgac ttgcttgaaa aggaatggtt agttcctaga
    28921 aaggttcgac actttttgcg tatacgtaaa aacgtttgga tcaatcaaca acctgctttg
    28981 ttccatcatg aagtaaaggg caatgaccag atcacattgt tattcgaaga aacagattat
    29041 aattatcaag aagttcaatt aggagatgcg aagcacatcc gtgttttata tgaagatgag
    29101 catttgatta tagtggataa gcctgcagga atgaagactc atccaaatga accgacagaa
    29161 aacagtacct tactcaatca tttagctgct tatctcgaca ctaaacagca acgaccttac
    29221 gttgtccacc gcttggacaa agaaacaagc ggtgccgttt tatttgcaaa aaatccattt
    29281 gtcttgccga tacttggaag aatgttggaa caaaaaatga tctatcggag atatcaagcc
    29341 atcgtatggg gagaaattgc caaagatatg accatcacca ataaaattgg acgagatcgc
    29401 catgatcgga gaaaaagggt cgtagatgat aaaaatggtc agagcgcctt gacacatgtc
    29461 actgtcaaca aagtactttc tgaacaaaca caagtctatt gtgttttgga aaccggacgt
    29521 acacaccaga tcagagttca tttagctcat aagggacatc cgatcgttgg agaccctctc
    29581 taccaaaata gaacagctga acgactgatg ctccatgcat tagaactgca catgatccat
    29641 ccatttactc aagaaaaaat agtcggtaaa gctttacctg gattatggga atagaaataa
    29701 aaaagaacag agtcttatca gcagataatg ataagactct gttctttttg tgtaatttgt
    29761 ctgcttactt ttgatgttcg tgttctgcta acagcaaact accgataatc ccactttcat
    29821 tcggcagtcc ccaacgaacg atatattcac ttacttgagg tgtttccata taaccaccca
    29881 tcaattcaac aaattgctga cggatttttt gaaggagatg atcctgattc attaccccac
    29941 cgcctaaaat aatcttttca ggtgctaacg tcaacgtata gttgatcagt gcttgagcaa
    30001 tataataagc ctgaatagcc caaacaggat gatcttgcgg cagatcttgc cctttgatcc
    30061 ctgtgcgtgc ctcaatagac ggtcctgctg ctaatccttc taaacaatct ttatgataag
    30121 gacaagtacc ttcataatgg tcatctggat ggcgtttcac ccaaatatgt cccatttcag
    30181 gatgtgcgat ccctgaaaaa atttcttcat tgatcacgac acctgcaccg attcctgttc
    30241 ccacggtaag gtaaatacaa ctattttttc cttgtgctgc tccttgcttc aattccccat
    30301 atgcagcagc attgacatcc gttgtccaag cagcaggaat attgtagcgt tccttgattg
    30361 tgccaagaaa atcgaattgt gcccaccctt ttttgggagt tgccaaaacg tatccgtaat
    30421 tatcttttgt tttgtcgata ccaatcgggc cgaatgagcc aatcccgatt cccttcaatt
    30481 cataacgatc aaaaaattca aatacctgat ccaatgtttc ttctggtgtt gtggtaggga
    30541 tacttaattt ttctaaaatt tctccaccat ctgaaaccgc acacacaaat tttgttccgc
    30601 ctgcttctat tccgccatac attcgttttc ctcctcgaat ccgattgtcc gaatctcata
    30661 aggttggatt attgggacaa ccgcttgctt cgttggttct tcaagcagat tcaatatgac
    30721 agtttttcca ttgtctttag ttatttccaa tttttctaaa tgactagaca tattgaaacc
    30781 tcttactact actttatcac taaacttgct gcgtttcaaa gctgtaattg caaacgtttc
    30841 actttttatc gttaaatatt gttgttttga aatcaatgtt ccttcatgat gtttgatttg
    30901 ttgagtggaa aaaggaatct gtgctgcgta tgcatgcaaa taagtagaga atttttcttc
    30961 tggtccatgt agttcaatgc tgtaattgaa ccgatgctca cctagacact gtgcttctgg
    31021 tgttggaaaa tatccccaat cgcctagttc tcccacactg cgaagtagcg tcactgcgat
    31081 ttgtccgtct tctgttactt catattcatt caaaccaaag tttccgactg ttacaccgta
    31141 ctcttcatca tgaatattga caaatgcttg ctgatgttgc ggattcgtgg ggttttccca
    31201 acttttagac acatggtttg ggcgcttcac cacttcaaaa atactatctg cttcgtgatg
    31261 ttccacatgt aactttgtag gaaacaagac acgtaagcga tgatctttca tctgattgtc
    31321 gagagtcgtt tcaaagtcga tttttttact atcttttctg attgtcatga ttgtttttat
    31381 ttgaaggatt cgtttttctt tggatcgttc tgcttttcga taacgaaatt ccacaaccat
    31441 ctgctgttct ttttctaaac gctcgtctgc agaaacaggg atttccattt cctgtagaat
    31501 acttattttt gctatttccg gtgtatctac taatactttt acttcagagt tttctttgtt
    31561 actggatagg attggctgat cacagaaagg ttgtttaaag atatactcat ttcctatatc
    31621 gccgatatct tcaaaaatat gcagattatt caactttcgt ttgtttttgc gatcttccat
    31681 tgtaatggta ccattttttt caatcgtcaa atgtagaggg ccgttttcga tgattcgtcc
    31741 actctgatga atcattgttt ccttttcttc tgctttcgct tccccttcta caagcgcgaa
    31801 tgtttcccaa gaaaatgctg gcatgttttc taatggtaaa gtcactttga tcattcttgc
    31861 catatatggc acacggaaac ggtctttcgg caaatcataa ccaaattgga cagtttcttc
    31921 agataaaata gctggtatag tggccccact ttttgtttct acatgatact tacgtttagg
    31981 caaattttct aactcttgat atagttgttc aggtattcct tcagcgaatc tttttctttc
    32041 taattcgatg gttatttctg cttcaccagt tttatccatt cctgcagtat ttacaatgac
    32101 aaatggaaag ctgtcttttg ggaatccggt cgtatcgatt gctgcggtca gctgttctaa
    32161 ggaatcttgt gcaagatact ttccaacttc atctgctttt tcaaaacgag gtatcatttc
    32221 ccgatggaca gaatcgactg aacaaccgca aatactgtcg tgaggatgat tttgcattaa
    32281 tgtcttccaa gcataatcta attgatcatg gggatagttt ccactaactt cataagccat
    32341 cgtcgcaagt ggttctgtaa tattttctag ctgtcgctgg accttggtat tccattgttt
    32401 taagtaaacc cgagcagatg ctgtattggc taaagtatac cagccatccg tttcttgact
    32461 tgtcaattct ccttctactg agcctaagtc ttctggcaca tctttttgaa ctgcttccag
    32521 atagtcagta aaattagaat gaataaactc gtaatctgga aacagttcat ttgcgagatg
    32581 gattgctttt gatatatctt tttgtaccgg ttgatgatca acaccattca tcatcaataa
    32641 gtggttcgta gaggcatatt gttctgcatc cgctaatttt tgcttccaaa aggcaagagc
    32701 tgcttctttt tctactggaa tctcattccc gttgctatac caattggcaa atagcagacc
    32761 aaagattgct gtttgatcag gtcctttcca ccacatctct gagtattggg aagaatagtt
    32821 ttctgcttcc aaaacttgat tatcaaagcc aattggtttt acaccacggc caaacgctgc
    32881 tgctgaaata ccagcttgtt tcattaattg aggcgtctgt cccatatttc caaatgtatc
    32941 gggaaaatag cctaacatga caggggttcc ccatttacgg ctttcttcca tgccaatcag
    33001 catatttctc acgttcgatt ccgagctgat aagaaaatca tcttgcaaga tataaaatgg
    33061 tccaattctc aattttcctt cattgattgc ttgttggatt gcctgtcttt tttctggcct
    33121 tacttgaaga tagtcatcta aaataatcgt ttgcccatcc aaatgaaaac tgttgaagga
    33181 cggatcaact tcaaatagct ctagtagatc gtctatcaat tcaatcagtc gcatgtgatg
    33241 ctgttcgtaa gccatgtacc attcacgatc ccagtggcta tgagaaatga tatatacttt
    33301 ctttttcatt tacgatacct cctacttttc tactctaata tcaaagtaat ccatgactaa
    33361 ttcgcaaaac atcatatttg cccaagaaaa ccattctctc gtataatttt caggattgtt
    33421 cacatcgaat ccttcgtgca taaggtgtgt tcctgcgtca gttgctacaa gtaaattcaa
    33481 tatccgctct ttttcagatt tgtctgacgt tgtcattcct tccatagcta atgcaatcgg
    33541 ccaaacgtat ttttctggcg tatgggagga tccaattcct tttgcgtact ttccttcata
    33601 gaaatatgga ttttctttac tcaacaatgt tcttcttgtt tgcagataat cttcctcttg
    33661 ttcagaacaa tatcccaagt aaggtgcggc gatcagattg ggcacattac tgtcatccat
    33721 gactgtggcg tttcccattc catctacttc atatgcataa atcgtttgcc ctttttgatt
    33781 aaccgttcgt ccgtgattct gaatcccctc ttggatctct ttcttcaatt tctgtgtttg
    33841 ctgtacaatt tctttatcat cgagtatcgt ttgaaaaatt tcttccacat aacctaacac
    33901 gaccaccgca aacatattgg atggtaccag atatccataa cggcaagtat catcgctagg
    33961 acggaaacca gaccatgtca ttcctgtaaa agctgttttt gcacctcgcc cgtcattgat
    34021 cagcgtatct tcttttcttg tcgtgtctcg tacaaatgta taaggagagt gttcatgatc
    34081 ttgctctgtt ttgaacacgt ccaagatttt tttgatacct tcaataaaag attgattgaa
    34141 ttgatcagtt tttcctgtat ttttgtaaag aagataggcc aactgtattg ggtagcataa
    34201 agaatcaatt tcatattttc tttcccagat ccaatcgttc atttttgtat gatctgtttg
    34261 gtggccagca ccgttagctt cttcgttgaa ggcattcgca tatggatcga tattgatata
    34321 aaaaaattgc tttttgacta acccgctgat catgtcagct aattcctcat cttcttttgc
    34381 aatcactaaa taaggacgaa cttgtgcagt agagtctctt aaccacatcg ctggaatgtc
    34441 tcccgtcaat aaaaaggtgg ttccgtcatt ttgctttttg actgttgtca acagagtatt
    34501 ggcaaatgct gctgtaaaat tctttgccca gtccgcatgg atatccccac atttttgtgt
    34561 gatatcttcc ataaatttct gcaccgattt tggtatatct ttataagcca ctaataaaac
    34621 cttctttcta tttgatatat ctttaaagat tatacttcat acagccaatt atgtctacgc
    34681 tttcaaaaaa tatcttttta tttacttttt catccattat ggtattattt gaatatgata
    34741 tatcattaag aggtgtttat atgaatcaac cattgtacca gacaatattt aatgatttgc
    34801 aaacggctat tttaactggt gaattacctg tagacagcca actaccaact gaaaaagaac
    34861 tttcacgtac ctataaagta agcagaatca cttccaaacg tgcattgact gaattagagc
    34921 aattaggact tatttatcgc gttcgcggga aaggcagttt cgttaaatcc gcttctccgg
    34981 aacagattct tgcttctcct acaaaaacac gacggattct gtttcttctt ccatatgtcg
    35041 ctgatttgtc tgttggtgat tttagcaagg gattgaaccc tgtgatgcaa gataaacaga
    35101 ttgatgtcat gatgacgacg cttgactttc tacaaaataa aacagcaaaa gagctaatca
    35161 atgaatttga cggattgatt tattatgctc ttgatacaga acaacatctt gacttacttt
    35221 ttgagctttc attgaaagaa ttccctgtcg tcatattaga taaaaaaatc tatgagttac
    35281 cttttcctac cgttctttcc gataatttcc aaggcggtgc gcttgctgcc tcttatttga
    35341 tcgaacaggg acattcaaaa atcgcttatt tgtttggtac gcagactcat ccacaatccg
    35401 ttcgacagcg ttaccttggt taccttgaag cattggacaa agctggtttg actttccaca
    35461 cttcactgga tgacaaaagc gcgacattag atgaattgct ttcttatatc actaagtatg
    35521 aagtaactgc ttttgtttgt gaaaacgatg ttgtcgctat ccagaccatg aatctcttga
    35581 gacagcatgg ttatcatatc cctgaagatt tttcaattat cggatttgat gatatccaag
    35641 cagcagctct tgttgacccg ccactgacaa ccatcgcaca agattttgaa cagttaggta
    35701 agcttgcggg agatgctttg gtcacttggt tagagacaaa agatcgacca tccgatagta
    35761 aatttccagt ttcattcatc aaaagacaat caacaaagga gaaataaaat gaatatcggt
    35821 caaatagata ctagacatgc aacagcgaat cagccaaatt tctctaatgg gaactgccag
    35881 ccttatactg gcgttccttt tgggatgaat tattttgcac cgcaaactac ggatcaaaac
    35941 ggcagctggt ggtttcatcc tgatgaccgc gtttttcaag gctaccgtct tacccatcaa
    36001 ccaagtcctt ggatgggtga tttcagtcat atgctgttta cgccgatcaa tggaaaacta
    36061 caggagaaca cccttttcca tgctcaaagt tcttaccgcc cagaagaaag tatattttgc
    36121 ccgactcatc tgtcgatctg tcaattacgt tatggcatct gttctacatt gatacctagc
    36181 atgtatggcg gaatcttatc tattgattac agcaaaaatg acagcggttt acttttatcc
    36241 tttccgggac gccatcagct ctttgttata gatccttaca cagtggaagg aaaaatcaca
    36301 cattttgctg gttcagaaga tcctgatttt tctttttatt ttatattgag atttgaatat
    36361 cctttaacaa ttgatgagtt agctgtttcc aacaaaaata gcgactcgat cccgttgtat
    36421 tttgaacaga caaaaaaaca aactatccga tttgggacat cttttatcag ctcggaacaa
    36481 gcagcttata acctggaact agaacaaaac caaagcaaag agacttacct tgctcacagt
    36541 caagaacaat ggcagtccta ttttgatcgt atacagctca cacaccatga tcaacaacaa
    36601 ttgcggacgt tttatcataa tttatatcgg gtgtttttat ttccacaaac attttatgaa
    36661 attgatccta gtgggcaaaa aatccactat gatacgacta gtcgatctat aaaacctggt
    36721 gttctctata caaataatgg gttttgggat acgtataaaa cggtttatcc tctttattct
    36781 ttgattgctg tagaaaaata cgaagaaatg ctggaaggtt ttttgaacag ctatagagaa
    36841 agtggctatt tgccaaaatg gctgtctcct gatgaacgtg gacttatgcc tgggacattg
    36901 atcgatgcgg tcattgcaga tgctgctgta aaagatatcc gacgagactt gatgcctgaa
    36961 tttctaacgg caatggaaaa aggtgctacg gttcaaagca aacatccaaa ttatggacgc
    37021 caaggaacag aagattactt gacatatggc tatgttccta atcactacca cgaatcagtc
    37081 aatcacacat tagactatgc ttatagcgat ttttgtatta gccaagttgc caaggtacta
    37141 gaagaagagg aaatgacgaa gaaatatcag cagcaatcat tgaactatca aaatatcttt
    37201 gactatgagt caggatttat gcgttcaaaa gataaagatg gcaagttcag agaaccattt
    37261 tcagctaatc gttggggaca agattatgca gaaggaagtg cttggcaaag cagctttgct
    37321 gtttatcacg atttttcagg actgatcgga aaatatggtg gcagtgagag atataaagaa
    37381 aaactcattg agctgtgcaa tcagccccca acgtttgacc caggaggata tggttttgag
    37441 atccacgaaa tgagtgagat ggccgcgata gaatttggac aattagctat ttcaaaccag
    37501 ccaagttttc actatcctta cttgttcagt tatataggac aacctgaaat ggcacagcca
    37561 ctgcttaaac agttgatgac tcagaccttc gacgattctc caatgggtta ccctggagat
    37621 gaggacaatg gaagtatggc cgcttggtat atcttcaata gtctgggatt ttatcctgtc
    37681 acgcctggaa ccggtgaata tgttatcggt atgccattaa ttgacaaggc tgttttgctt
    37741 ttgtcaaatg gaaaggaatt gacgattgaa gcaacgccta atcggccgca gcaacaattt
    37801 atccataaaa tcaaacggat ggacaaagaa tatacgaaat tatattttac ccatcaagat
    37861 ttgctgaaag gcggcacgat ctcttaccaa ttagggatca tccctcaccc taagaaatat
    37921 caagcaaaag atttgccttt ttccttatca aactataatt aacaagaggt tgtgaaaaag
    37981 ctgtcctgca tcaagtaata agaacagcca agcaaaaata gatcctcata tttttcgttt
    38041 ggttggtctt attaccgcag atgcaagctt ttgaacaccg tttattgaga ggttgtgaaa
    38101 tcagcgttct gacctgagta ttagagagac aaatggcaaa atagttcctc atattttgcc
    38161 attttttgag ctaataccga gggtcagctg attgaacacc gtttagtgga gaggtcgtga
    38221 aaaagctg
//
LOCUS       DACCCS010000020        36794 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.020, whole genome shotgun sequence.
ACCESSION   DACCCS010000020 DACCCS010000000
VERSION     DACCCS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.020"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            355..1173
                     /locus_tag="IVK82_001103"
     CDS             355..1173
                     /locus_tag="IVK82_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6392178.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            1192..1599
                     /locus_tag="IVK82_001104"
     CDS             1192..1599
                     /locus_tag="IVK82_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392179.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            1919..3004
                     /locus_tag="IVK82_001105"
     CDS             1919..3004
                     /locus_tag="IVK82_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP6392180.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            3841..4173
                     /locus_tag="IVK82_001106"
     CDS             3841..4173
                     /locus_tag="IVK82_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP6392181.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            4764..5093
                     /locus_tag="IVK82_001107"
     CDS             4764..5093
                     /locus_tag="IVK82_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392182.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            5080..5421
                     /locus_tag="IVK82_001108"
     CDS             5080..5421
                     /locus_tag="IVK82_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP6392183.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            5535..5774
                     /locus_tag="IVK82_001109"
     CDS             5535..5774
                     /locus_tag="IVK82_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392184.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            5841..6080
                     /locus_tag="IVK82_001110"
     CDS             5841..6080
                     /locus_tag="IVK82_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392185.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            6207..6896
                     /locus_tag="IVK82_001111"
     CDS             6207..6896
                     /locus_tag="IVK82_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAP6392186.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            6902..8068
                     /locus_tag="IVK82_001112"
     CDS             6902..8068
                     /locus_tag="IVK82_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP6392187.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            8150..8821
                     /locus_tag="IVK82_001113"
     CDS             8150..8821
                     /locus_tag="IVK82_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP6392188.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            8874..10850
                     /locus_tag="IVK82_001114"
     CDS             8874..10850
                     /locus_tag="IVK82_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6392189.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            10863..11615
                     /locus_tag="IVK82_001115"
     CDS             10863..11615
                     /locus_tag="IVK82_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6392190.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            11631..12392
                     /locus_tag="IVK82_001116"
     CDS             11631..12392
                     /locus_tag="IVK82_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392191.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            12405..12665
                     /locus_tag="IVK82_001117"
     CDS             12405..12665
                     /locus_tag="IVK82_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392192.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            12662..14752
                     /locus_tag="IVK82_001118"
     CDS             12662..14752
                     /locus_tag="IVK82_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6392193.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(14788..16845)
                     /locus_tag="IVK82_001119"
     CDS             complement(14788..16845)
                     /locus_tag="IVK82_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP6392194.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(16990..17181)
                     /locus_tag="IVK82_001120"
     CDS             complement(16990..17181)
                     /locus_tag="IVK82_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392195.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            17369..17824
                     /locus_tag="IVK82_001121"
     CDS             17369..17824
                     /locus_tag="IVK82_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392196.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            17821..18063
                     /locus_tag="IVK82_001122"
     CDS             17821..18063
                     /locus_tag="IVK82_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392197.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            18081..18383
                     /locus_tag="IVK82_001123"
     CDS             18081..18383
                     /locus_tag="IVK82_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392198.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            18452..20506
                     /locus_tag="IVK82_001124"
     CDS             18452..20506
                     /locus_tag="IVK82_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392199.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            20506..23118
                     /locus_tag="IVK82_001125"
     CDS             20506..23118
                     /locus_tag="IVK82_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP6392200.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            23115..25493
                     /locus_tag="IVK82_001126"
     CDS             23115..25493
                     /locus_tag="IVK82_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392201.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            25554..26096
                     /locus_tag="IVK82_001127"
     CDS             25554..26096
                     /locus_tag="IVK82_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392202.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            26174..26506
                     /locus_tag="IVK82_001128"
     CDS             26174..26506
                     /locus_tag="IVK82_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392203.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            26507..27100
                     /locus_tag="IVK82_001129"
     CDS             26507..27100
                     /locus_tag="IVK82_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392204.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            27122..29146
                     /locus_tag="IVK82_001130"
     CDS             27122..29146
                     /locus_tag="IVK82_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP6392205.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            29146..30345
                     /locus_tag="IVK82_001131"
     CDS             29146..30345
                     /locus_tag="IVK82_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP6392206.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            30361..31005
                     /locus_tag="IVK82_001132"
     CDS             30361..31005
                     /locus_tag="IVK82_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392207.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            31016..31822
                     /locus_tag="IVK82_001133"
     CDS             31016..31822
                     /locus_tag="IVK82_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392208.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            31845..32074
                     /locus_tag="IVK82_001134"
                     /pseudo
     CDS             31845..32074
                     /locus_tag="IVK82_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32233..33168
                     /locus_tag="IVK82_001135"
     CDS             32233..33168
                     /locus_tag="IVK82_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAP6392209.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            33212..33481
                     /locus_tag="IVK82_001136"
     CDS             33212..33481
                     /locus_tag="IVK82_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392210.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            33757..34032
                     /locus_tag="IVK82_001137"
     CDS             33757..34032
                     /locus_tag="IVK82_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392211.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            34074..34496
                     /locus_tag="IVK82_001138"
     CDS             34074..34496
                     /locus_tag="IVK82_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392212.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            34516..36108
                     /locus_tag="IVK82_001139"
     CDS             34516..36108
                     /locus_tag="IVK82_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392213.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            36129..36464
                     /locus_tag="IVK82_001140"
     CDS             36129..36464
                     /locus_tag="IVK82_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392214.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            36610..>36794
                     /locus_tag="IVK82_001141"
     CDS             36610..>36794
                     /locus_tag="IVK82_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392215.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT        13368 a         5607 c         7350 g        10469 t
ORIGIN      
        1 gtatatgatc taagtcttgc ttctagtatg aatatgaccg atgtatacga aaagttatta
       61 atactagaaa atgaatatga tcaattcaat tcaaatcagc agcgaaaagt aaagaaacag
      121 atagactaga atagctattt tcgaaaagca atatttaagg tataagttat acctttaata
      181 ataaaagtat aagttatacc ataaatatat atggtataac ttatactttt attattaaaa
      241 accgctatat ccaaacgttt ttagatatta ttatttaagt tatactttta atgttgacca
      301 tttccgatat ataagttata ctttaattat caattaaaga ttgggaggat ttacatggat
      361 acttttcaag ggactacatt tacagttggc aattttaaag gaggagtagg taaaactaag
      421 atagtcagca tgcttgctta tgacaatgca atgataagaa atagaaaaac gcttattatt
      481 gatttagacc ctcaagcgaa cttaacacaa gtcatagcaa gaactttcgg tataaccgat
      541 atcaaaacca ctataacaaa tggtgtcgca aatggagaat tagcaagtag tattattaag
      601 gtaaatgaaa atcttgacct tctggcatgt gacactagtt ttcgatcatt ctccgctttt
      661 gctaataaac atgaaaatga attagaccaa gttactgtct tgaaaaaatt acttcttcca
      721 ataaagaaca attatcaaga aatatttatt gatgtaccac caaccattag cgaattttcc
      781 gataatgcta tggcagcttc agactattcg ataatttcat ttcaaacagc tgaagaatcc
      841 ttagagggag tcaacaaata tgtaaattat caaaaattca tggtcgatag atacgatctt
      901 gatttgcaaa ttatagatat tgtaccttgt atggtagaac caaatgatga ttttgatgaa
      961 gaaatcctag aagaagcaat tgaaaaatac ggaaccgtgg tatccaaaaa tctcattcat
     1021 tatcaaaaaa gattgagaag gtattctaaa attggtatac atttaagaaa atacaaaaat
     1081 ggaaattttg atcaatggga ttataaagca caccaagtat ttataaatat tttggcagaa
     1141 ctagatgcta gacaagagtt tttaaataaa taataaggag ttgcataagt aatgcctaaa
     1201 gattttgata ttcctaaaat gatggagaat ggtgaatttg agccattatt acccaataac
     1261 aaacgccaag atgtgaaaat aagcggtgaa tataacttta gcaatagtgc attgagtaac
     1321 acaagaaata ttacattgaa aaaaagaggc cgaccagttt caattcctga taaaagaaac
     1381 aaggtatcag ttcctaaaaa gatctctccg gcattaaata gcaaactctc aattttacaa
     1441 gactatatga cggagttaca aagtgaaaaa gggagaattt ctttcgaaaa aattgttaat
     1501 acattagcag actcatatat ccagcataga ctaggagtag caaaagaaga acacataaga
     1561 gaagaaatac aagaagagtt tgaaaaatta ccaaaataat tttttttcat gcggtattca
     1621 attaaagtat aagttatact agataaatga atattaaagt ataagttata cattaaatat
     1681 ttaaggtata acttaataaa caacgtgtaa atttgcgatt aaatcaacat tataaaatta
     1741 ttatttaaag tataagttat acattatttg tatttaaagt ataacttata cttatttttt
     1801 taatatataa cttatacctt aaatactgaa agtgttttat tcacccttaa tacatggtat
     1861 agttcacttg ttgaaaatcc tgaatatata caagtttcta agaagaagga gaaatttaat
     1921 gtctacagaa aatcaatctc aaaaaatgat aattaaaaat tggggatata aagcaacgaa
     1981 tgtttatatt gaaaaatatt ataaatttcc tagtttttta attcagaatt ctaaatactc
     2041 taaaatgaaa gacagctcaa aaatagccta tatgcttttt aaaaatgaat ttcgtagagc
     2101 cttacatgaa aaatggatcg acgatgatgg tctattatat ttggaattta cacaagcaga
     2161 cctaatggac aaattaaatt gctaccaagg caaagtaaaa accattttaa acgagttaga
     2221 aacatataat ttgatttcag tcgtaaaagg agaatttaac ccaaaaacga aaaaaaatga
     2281 aaaaaataaa tactatttac ttcaacctga aataagtgcc gatgatcttt ttattcaaaa
     2341 ttctttcgat gacgatttaa gcgaaactat tgataaatca acctcttctc ggaatgcgga
     2401 aatcgcacgt cgagaggaaa ctgctaaaac ccttgataac actggaaatg cggaaatcgc
     2461 acgtcgagaa aaaacagctg aaacccttga taacactgga aatgcggaaa tcgcacaaga
     2521 ttattatatt aataaatctt tagacactaa aagacactta aaagacactg aaacagacca
     2581 attacaaaat caagttttgc tagataactt cgttgaaatc atgaaagaag acagcataaa
     2641 cacatttgta ccagaaaagg tattaagctt gataaaaaca ttttctaaca cttacgaaga
     2701 agcccaacaa accgtcacca caatacacaa agcaaaacat aaagccgagg aacttgagaa
     2761 gacggtagtt gtgtatgaag aacttgaatc ttacggaatt gatgcggata aaggattata
     2821 tatgacgttg ctgaaagctt atcaaaaaca aaagactgag aaagtaaata atttgcagaa
     2881 tttgattttt gtgtatgtaa aaaactggtt tattgaaaaa gctattgcgg caaagcttgc
     2941 aaacgaacaa agagaaacat ttaacgaatt accaacagtt agtacggaaa attggctaga
     3001 gtagttgata aaaagcgaac actataaaaa tgatcaattt taaattagaa cttttttaca
     3061 attttaattt tattacaaat gttacaaata aatagttatt attccggagt ttttagatta
     3121 aaagtttatg aaaacaatca caaacgtagg ttttaatgtc ataatcgtta gatattttag
     3181 ttatttttca ttcaaaaaaa cagggaacta gtgtttgcat atgtattttt ttgagttata
     3241 ataattcatg aagagaggta attcacttat ttacccaagc tgaaaagcct gatattactt
     3301 cttttcaggc agtgctgcta atattcaatt acactttttt gtataaattt agttgtttta
     3361 aatttaattg tctgctatgc ttgtgatgaa ttgattttcg ttctgcactt ttggacgata
     3421 aatttggaca attgaaaata caaaaaaaag aaaggccctg tgagactcgg aaggctacca
     3481 accttcgtcg acgtccacag gtggcattaa gtctataccc cttaatgcgt ttacctttca
     3541 gtgttacggt attacccgta acggatttag tataacagat attcaaaagt gataccactt
     3601 tttttggctg tgttaaggtt aaaaatacgt gatattacga tgaatctatg cctaagcata
     3661 atgttataaa aatgtccgtg gggctctttg ccgtatgacg aacgctgaaa ttagagggaa
     3721 cgcatgtttg aatgcgttcc ctcttttttg tttgtcatga acgaaaatca attctctatt
     3781 tttgaaaaat aacaagaaaa acttgttaaa aatatttaaa gtaagtgggt ggattatttt
     3841 atgtcatata caggaatttt gagtctcgaa gatatttgtc actacggtaa acggtgtacc
     3901 gctacagaaa agatcacgaa gaaactatca actggtcaaa gtaaaaccgt tgtgcagtgt
     3961 aagaaatata taatccagaa agacaaagtc tcagaagaaa tgatttatta cattgggaaa
     4021 caaaaacaaa taattttaaa agatcctatc cctttaaaag agctatatcc aacaatcaaa
     4081 catgtttatg accaaaacgg tgtactgatt ggtcgaagaa aaaatggagt cttgcgttgt
     4141 accgcaaaag ggatggggcg tttaatcagc tgaattgatc attctcataa cttgtatttt
     4201 atcgtttttc gcttgcttga aaatattgtc gagacttcta aaggaactta gaatcactag
     4261 aaaaataagg agtagacttg atctatcaac aattcccttc ggggagtgac ggggtaggtt
     4321 tttacgattg gtaacgtaaa aaattggtca atcgagtaaa tgcccttgtt tggatagtgg
     4381 tgaaatgaat ttgtgtaaat aatatgcaca gagtaaacaa gagggaattt agaagttaga
     4441 caagttagaa tagcgcctag attaatcatg agcagctact aagggatcta ggctttgacg
     4501 caaactcatg gaataaggag taggtcttct cgtaatgggg aggacgcaca agaaattgtg
     4561 tcgtgtgctg cagtacatgt agatacttgc gaggacgttg atcattggat caatcgagca
     4621 tttaccgctg tctactaggg tttctcgttt cggacgccat aaacgagtcg atttatttta
     4681 tatatttttg agtgaacgaa aaacgataaa gatattcgtt atgaagtaaa actaatttag
     4741 tagatagcaa aggtggtaga ggaatgattc aaaaatttgc gaaaatgagt aaaactgcat
     4801 ggattgaaaa gcttgaaaat gacttgagtg tcatgcgatt agctccaaag aaattcaaac
     4861 gagagattgc agaaaaagaa gcaattttgt cggtattgaa tgcggtagac gataaatgtc
     4921 cagtaaaaat tagtttttca gatatagcaa caattatcga aggtatttat tatggtgaag
     4981 ttgatgaaga gatatcttct acaaaagatt tattagattt tttggataaa aacaaagata
     5041 cctttatagt actaactgat tatgaggagg ttacaacaaa tgaaaaatta tgatccgaat
     5101 atacgattgg gcacacatac gattaaggtt tctttccaac gttgggatta caaaggattt
     5161 gtgacgttta gaagaggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat
     5221 gatttatacg atcaaacatt gactgataat ccaattggat tcggcttgct acctgaagat
     5281 gatgaaggta atgaatggtt taaaatgacg cttacggatg ataatggaga cgaattatct
     5341 gttgaagata cttggtcata tttgagtgat tatattgtca gcgttgaaat cattgaattt
     5401 gtagcggata aagaagaata aaaactggag gaataaattt tacagataaa ggcttagaag
     5461 gagtcacgac gaataataaa aaggtagtat taccttaggc attgagtaag gaatttattt
     5521 aggaggaaaa ttagatggcg cgaccagttt tagaggcgat ggatatggat actttgacaa
     5581 aaggaaggta tgtacagctg ttagctcgtg gttattcacc taaagatgta ttcaaatccc
     5641 tgtctaaagg aacagatatg gaaaaagaaa gattacgaaa agaattcgat tattggagaa
     5701 cccataatgg aatcaaggat ttgaaaccag cacggccagt aattaaacga aagaaaaaga
     5761 taacccaaga ctgaagtgta gagcgtatgt gatcataggc ttttttattt ttgcaaaaaa
     5821 caagaaaggt aggaaaactc atgaattatg aagaaatcga aaacagaaaa aaagtgtcga
     5881 aagaaatgga agaaaagttg ctaaaaatga tgaaacaaaa acaccttaaa agattatctg
     5941 tcatgcaata tatcaatgat atgaagataa caggaaaaga gaaagcttgt ttgttaggtt
     6001 caatgaaaaa ctttgaacag ttgaggcgaa cttatgtaaa aacaggttcc aattgccaac
     6061 tattattaga agtttcttga cgattatgat atgaaaatga tcaattatga tgtggcattg
     6121 ttattgaata aaaagaatag taaaatttaa catggtttat cttggttaga tgtaggtaaa
     6181 ttaattcata tatagaaggt agatagatga ataaaaacaa taaaacttta tttaatcctt
     6241 acgtgggcgt aatcattgtt tttcttcttt ttagttattt tgcttatcaa ggttatcaaa
     6301 aggacaaaat cgctgaaaaa gtagatggtg taacaaaaac aatccatatt caggaacagg
     6361 ctaaaaccta tcaagataat ttaaccagtt taacggatat ttctaagaga caactgatta
     6421 ctaatcaaaa acaggcatta gaaaaggatc tcaaaggtga aattgctgga aaacttgttt
     6481 tttcttctac aggatttatt ctccctgttt ttcaaggagc gaatagcgac acgcttagtt
     6541 taggagtagc ttctacttat tatctagata ctgaaatggg aaatggaaat tatattcttg
     6601 ctggttataa cgtagataca ccaagcgttt tattatccga tatgactgat ttatctgaag
     6661 gagaagaaat tgatctaatc gatgctcatt acaagtatca gtatgaagtc tccaaaagag
     6721 gaaaaatttc tgattacagc caggtagttc aatctaagga aaacaattcc tttttatcct
     6781 tgccaaaagc aggtaaaaaa ccactactca cactgttagc ctttcaagca aataatatga
     6841 aaaagtgcga tgtgatccag tgtgaattag tcaatgtaga aaaaatcaat aaataaggga
     6901 agtgagtcac attcaagaac ttgtagaaaa attgatcaat aaaatgagtg ccatttttct
     6961 gttactgttg gtatccattc tatatttaat atggacggtg gggttaaatc ctcctacgat
     7021 attatttatt tatgataatg ctgcacagtt agtgacgttt ttcgtaggct gtttgtttct
     7081 tttgaatctg tacaaggtaa agagaaacga tctattcttc attgcagcag gcattctttt
     7141 ttaccttttc ttttcttttt ttagaagcta ccgaggagat actttctatg gtgatctgat
     7201 cgttccgaca attattgcta ttattttgat tgtaaaatgg acgaaattta ctgtaattga
     7261 tcgtagcttg tatcttgtag tattctatgt ctttttactc gtaacggttt atcgagtatt
     7321 tactgagata ccagtaccag aaggacaaag tatttggaat ccatcgaaca aattatcaga
     7381 tatttggatc aatacaaata cgataggtag ttctttaatg acattagccc ttcttatcac
     7441 aggatttgct agttcatttg gaaaatggta catgcgattg ctatcattac cagcgttgat
     7501 tcttgctggg gcaacgattt gggtttgcca atcgaaagca gcattatatg cgcttgttat
     7561 cttcattatc ttagaaattt tacctaagaa ggtttttcga aatacacact tgtccttttg
     7621 gatgtatgca gcagtttcgg ttctagcttt accgatttct tattttgctg caatgtcaga
     7681 caaagttcac ttatttacag gacgagaaga aatttggcgc aaattctatg aaacattagg
     7741 cgaaaaaaca ggacaaatat ttgtagggat gaaacctttc tttttccata gaggaaatca
     7801 gattctaggt aatcataata cgtatcacgc actcgtcaat cactatggat tgattggttt
     7861 tgttctatcg attattcttt tgcttagttt cttatggttg ctcgtaagaa aacgtgagct
     7921 gacaaatgga caactaacat tcttgtgggc ttttttagcg atcatgtgtc aatcgttcat
     7981 ggaagaaaca ttaacgacaa tcagttgggt gccaattgta ttcttaatgt tagcaatggc
     8041 tacacaccaa tataaaacca ccagataaat gaaatagaat agtaataaga gaaaacactt
     8101 tttcgttgtg aaaaagtgtt ttttgttttg tctgaaagga ggcagatgga tgaaaaaatg
     8161 gttatttggt tttttaggag tcgctttaat cgtcgtttgc agtgtgtttg gctatgttag
     8221 ctaccaaaaa catgaaggag aggtgtttaa gcaaaatata gaaaaaaaga tgcccgtaga
     8281 tcaaatcaat gcacacgcaa agagttataa ggaggatgca acgaacgtca ataatgatat
     8341 gtctttgggg caaatgttgt ccatacaaaa agaagcaatc gaaatgggcg tcaataaaca
     8401 agtatttgca caaattcaga ttcctgcact tggtttagca ttgccaattt ttaaaggggc
     8461 aaatcaatac actttgagtc ttggagcggc tacttatttt tatgaagatg ccgaaatggg
     8521 taaaggaaac tatgtcctag caggtcataa catggaaatg ccaggtgttt tattttctga
     8581 tattcaaaag ttgtcattag gggaggtgat ggacttagtt agtaacgatg gcgtctatcg
     8641 ttacaaggta acgcgtaaat ttatcgtacc tgagtacttt aaattaatcg atggcgttcc
     8701 tgaagagaac tctttcttgt ctttaccaaa gaaaggagag aaacccttat taacgttgtt
     8761 tacttgtgtt tatacgtcac aaggaaaaga aagatacgtg gtacagggag agctacaata
     8821 acaagaattt aatcaacgcg agtcgttgaa atatgaaatg aggagagtaa aaaatggcaa
     8881 taaatcggta tgtaaaaaaa gctggaatga ttgttggaat gttaggaatc attgcaggct
     8941 gctttcaggg gtatcgagct tatgcagaag atgtcacgca aaaaacgcca ccagaaaggg
     9001 tcaatatcac ggttcataaa ttaatgtacg atcaaagtac gcaattgaac gttgatattg
     9061 acggaatcaa aaatgatggt tatacgcacg atcaatatcc tacaggtgta acgaaataca
     9121 acaaggcaga ttatggtgat gttgagttta cgttaggaaa tattacggat caagtattac
     9181 cgactgaaga ttctgatcta accaacgcga aagtggacga aatcgttaaa gatgtagaag
     9241 acaagggatc taacagtgag tacgttaaga atgcaaccaa taaaattact tcagctgtag
     9301 atgaaaatgg tgagatcact tttgctgatc aaccagccta tgtcaattcc aaagggaatg
     9361 tatatgtggt ttatgaaagt aaatccgctg ctggattagt cacacaaaaa gcaaaaccaa
     9421 tggttgtgat tgcgccaatg acagacaaca caggatcagg atttttaaaa gatattcatt
     9481 tgtatccaaa gaacattgtt tctaagttga gttttgaatt aacgaaattt ggtgacgatg
     9541 gcacagctca aagcaaacaa acacctttaa aaggggcaaa atttgagtta tacaagggcg
     9601 aacctggaaa aggaacaaaa ttaggagagc ttgtatcgga tgatcaagga aagctgactg
     9661 caactgattt gacgttagga aaatactact ttgtggaagt tccttcagaa gttgtagttg
     9721 gatcagatca agaaccaaca gcagaccaat atttgttagg cgcagacgct cgcaacgatg
     9781 cgcacaataa gttaaccttt gaaattacga atgacggcgt gacaagtgat ttaaaagctt
     9841 cctacgtgaa ctataaagca ccagtgatcg ataaaacagt gacaaatgga acaggagaag
     9901 aacattcttt ccaaattggt gacgcagtga attatcaagg tacaattcat atccctacag
     9961 atatcgcagg tggaacagat gggattacag taaatggtgt aaaaagtgaa acttctcctt
    10021 acagtgtatt taaatggggc gacacagcag gtaaaggact ttcttatgtt gctgaaaaag
    10081 cagatattaa agtaacaaat aatgatggtt cagtcgtatt aaaagaaaat actgactata
    10141 aaatcgaaaa ttctgatagt ggttttgtga tcgattttat cgtaaataat ggacaagtaa
    10201 gtgatacagt tgcgaattta catggtcaag atctaaaaat gacttataac atgtttgtga
    10261 atgatagtgc tgaaatcgca aatccattaa caaatagcgt ggatttcgtt tataacaata
    10321 atccatttaa tcaggaagaa caccacgaaa aaacgaaagc tgacgttgtg acttatggcg
    10381 caaaattctt aaaagtcgat agcggcctat ttggaacagg tatcaaagca acaccgcttg
    10441 aaggcgcaga atttgcagct aagaatgcag aaggtaagta ctacggtgga cttactgata
    10501 cagataaaga tggtgtcaaa gaagcagttt gggtagacga tgtagcaaat gcagcaatcc
    10561 taaaatcaga taaagagggt cattttgaaa tcactggttt aactgaagga gattactcac
    10621 tggaagaaac aaaagctcct gaaaactatc aaaaattaac aaaagagatc tctttcaaag
    10681 tagacaaaga ttcatacaaa gaagaaaacc gaatcacgat caaaaataat caaaaagctt
    10741 ccgtaccaat gactggtagc aacggtttcc aatcttacgt attgatctct tgcttgttgc
    10801 ttggggcagg cgcaatttct gcagttgttt actttaagaa gaaagcctag aaggatcagc
    10861 taatgattat cagacaccct aagaaaaagc gaatcatggg taaatggatc attgctttct
    10921 ggttactttc agctgttggt gtcttacttt tgatgcctgc tgaagcgagc gtggcgaaat
    10981 accaacaaaa tcagcagatt gccgcaatcg atcgaacagg aacggcagca gaaacggatt
    11041 cttctttgga tgttgctaag attgagttag gcgatcctgt tggtattctt acaattccaa
    11101 gtatttcttt aaaactacca atttatgacg gaacttccga caaaatttta gagaatggtg
    11161 tagggattac ggaaggaact ggcgatatta caggaggaaa cggtaaaaat cctttaatcg
    11221 cagggcacag tggactttat aaagataatc ttttcgatga tttgccttca gttaaaaagg
    11281 gagaaaagtt ttatatcaaa gttgatggtg agcagcacgc ttatcaaatc gatcggattg
    11341 aggaagtgca aaaagacgaa ctacaaagaa actttgtcac ttacttagaa ccaaatccca
    11401 atgaagatcg agtaacgcta atgacatgta cgcctaaggg gatcaacaca caccgtttcc
    11461 tagtgtatgg gaagcgagta acctttacaa aatcagaatt aaaagatgaa gaaaataaga
    11521 aacaaaaatt atcttggaaa tggcttttag gaagtactgt ttttctttca gtcatgatca
    11581 ttggttcgct atttgtttat aaaaagaaaa aataaaggag ggtaaccaac atgagaaata
    11641 gaaaatggct atttgtttgt gcagccgctc ttttttcttt cactgcctac acacagttga
    11701 cgtttgcaga cagacaagaa agcacggata gcacagaaca aacaatccaa acgtcagata
    11761 gcagcacgat tcaaagcagc gatgaaaccg tcgattcgtc aacagttgat tcttcagaag
    11821 aacctaaaat tgctgaagac gatcaaatag tcaaacagac cattcacgtt caaaaaatta
    11881 tttcttccga acaattagac aaccaggggc aaaagcttga aaaacaagaa ggagtaaatg
    11941 gcgcgaagtt ttctgtttat gatgtatcgg acattttaca aaagatggat gttaaggatc
    12001 tcactactga ccaaattgaa tcccaattaa aagatcgagt gaaaaaacta tccagtgatc
    12061 aactgaagct aatttctaat ggagaaacaa agacaattga tcaacaaaca ggtgtctttg
    12121 aattctcggt agaagtacag gcaaatcaga aacaagctta ttatattgtc aatgaatcaa
    12181 gtcctgaaaa catttctaat agcgaggata ttttgctatt aacacctgtt tctgataaaa
    12241 atggtgaatt cttaaaggat gtgtggattt atcctaaatc agaagcttcg caacctaaag
    12301 aagaagtaaa aaaaatcgta tctactggcg taaagaaaaa cttctttgag aattgttggg
    12361 attttgttac tcacttgttt tctagagatt aaggaagaaa taaaatgaaa ctgatcaaat
    12421 tattattatt caatttttct tgtttaaccg cagtcttttt gtactgcggt ttattatttt
    12481 ctaaactatc tattttgcca agctaccaac tatacctaat gaggatttta aacataccag
    12541 ctgcttccat gccgctagat attatggaaa agctcattag gaatccatta tttactacga
    12601 tcaatttgat cgttattgtc ggaatttcac tagtcattta taaaaaagag gggatgaagg
    12661 gatgaatttt aaacactcgt ggacaagacg gttaaaatat gtttttccca taatgatgat
    12721 gttaggtaca ttgtttgggt taaaaacagc caatgtcttt gctgaaaaag ttattgtcga
    12781 tccgtcaaat ccaatccaga acgtaaagaa taattttatt attccaaaag atgttcctaa
    12841 tgcaaaaaca aatattactg taaacggagc gtcaaaagtt tacaaatatc atgttgatgc
    12901 caacaaaggt ggttttacag acgactacgt aggattagtg gagggaaaca aaaatatacg
    12961 ctatccttct ttccaaaaag aatacggtga aacaaacctt gttttagaag gcgtaacaac
    13021 caacaccaaa gtcaatattg actatggaaa aatcggtacg tataacggga aaaaagtcaa
    13081 tatcaagcta gttctatcta atattcactt atactcggat acgttgccgt ggaatctact
    13141 agataacaac tatacaaaaa ctcattttcg agacgatggc tataaaaata cgaatggtgc
    13201 aatgagtaaa tctaaaaaaa gaacagtact ttggatctca gacaatcttt tctcaggaat
    13261 tgtttaccat tcaacacaaa tgaatgtgca gctagtagcc acatatgagg acggtagccc
    13321 cgttcaattt tcaggagata catttatcag ttttaactct ttgaaccctg caggaggaaa
    13381 aagcacggat cttaaaggag aatatgccca ctatgacaaa atgaatacga cagattggta
    13441 tgtcagaaaa gatacggttc tttcagaatt taaatctttc tacaacaatc tgaacgtggt
    13501 aggtggccac ccaggaggaa gctctaagct gacacaagcg gataatgatt tcaataattt
    13561 gcatgataaa ttaggagatc cgaagtttgg tcaaggtact gtgtccttta aaatatctga
    13621 agcaaatccg acgtttgtga ttggttcttc taatgttcaa acctggttta ctttatcttc
    13681 tgcgacaatc tttagtgttg ttcctgatca acctgaaaaa acaggggttg ataaaaatgg
    13741 aaataacgtg aacgataaaa tgttgcaagt aggagacacg attcaatatc gaatcaaaca
    13801 gaaggtgaat cgattaggag tggatttact agcggtttac gatcgatttg agttaatcga
    13861 taatttacca aaagaagtaa attacgtcga tgcacacgtt gaaagtggta caaataaaaa
    13921 attcgatgta tctggtgaag tgacctatga caaaacaaaa caccaggtaa aatatgcagc
    13981 gaaagctgat acattaaaaa atcgaatgaa gtataacggc gaaacttacg aattagtgat
    14041 caatgtaaaa gtcaatgaat tggccaatca aaatagtgta gccaaaaatc agggaacgtc
    14101 gatcattaat aaagtagaaa aggatacaaa tatcattacc gtttatttcc ctaaaattcc
    14161 agtgaaagaa gtacaacaaa atggaaaaga tgtaaacgga cgaaatgacg gtaagaaggg
    14221 tacgccgaca gctcctttaa acgcaggatc tgaagtacaa tatctagtca cacaaaaatg
    14281 gcacacaaaa ggcgtagacg ctgcgtcaga tcactacaaa caatttagta ttcaagatcc
    14341 aattgaagct cgtttgacat ataaagaagg ttctgcgcaa gtaatagata agagtaaagg
    14401 aaaagatatt acttctgaag gcacactaac ctatgatagc aattcaagaa cgctgaagtg
    14461 ggaagcttcc gctgacttct taaataacaa tcttttagat ggacgagaaa ttcagctgat
    14521 atttacagct aaaactccat tacaatcaga aaaaaatatt gataaccaag ccgtagtagc
    14581 agtagataat gtttctaata aaacaaatgt tgtgacaatt ggggttgatc ctaacttgcc
    14641 acaagttatt gttcctaaaa caggttctac acatttagta acgattttag cggtcagctt
    14701 actattactt gtgttagcaa cttttggcta cgcagttctg aagttcaatt aaacaaaaaa
    14761 acaaggctga aaatcagcct tgtttttcta tttgaaaatg ggattggcac taataaatac
    14821 gttgtctgaa gaaagtgatc cgtctgcaga aacttcataa gcatgggata aaccaacgat
    14881 actttgattg ttgtaaagca agtttgcgtt atgagcagcg ctatctttcc acataaccat
    14941 agcatttact gctcctgtat ctgaaaggat ctcgtcaacg taaaggtaga aactattgat
    15001 attttctcct ttagcaccgt agcagcctaa gctttctaag agatcttgga aagtaccgcc
    15061 gccaggtcgg taatgactga agcttacttg taattctttt gcacgaatat ctgcagcttg
    15121 ttgaacgata tcattgaatt gcattggggc aacgccattt tgttgacggt agctatttaa
    15181 ccaagcaagt tctttttgac gcacatagtt gtttaaatca cttagtgaga tatttttgta
    15241 tttgaaagtg atcgttcgat tttggtcaac attattgatt acacgttgtc catttttatc
    15301 aacggtaaca gatgaatcgt catttctaca caagaagtag ttttgatcgg tcatattttt
    15361 ccaagaagta gagaaactat ctccctcgta aaccgtttgt gtttcagtag ataaaacagc
    15421 gtttgtgtca acgtttatat ggttcacagt gacgttgtat tgatgagtat aggcaaaatt
    15481 gattccttga ttacttgtaa cgttgtttaa agtcacagtg ttgctagagc tttctctagg
    15541 atcaagaaca aaattagtga acataggtgc ttcaattgtg atgctttgtc cataaacgac
    15601 gggataactc ttactgtaat tatagagttc ttggatattt ggatcagtat ccgagacttt
    15661 atttggacga gctttcactt caactagaaa gacttcatca tagtaaaaag taaatgtggt
    15721 atttgtgtta atcgtttgtg tttgtgtttc attcccattt acttgatact gtggtgcttc
    15781 aaagttagtc aagtcggttc tagcttttgc agtgtaactt gtaccagctt ttactgagac
    15841 atgttcttct tttagcagcg ttccagtttg tcgattcata tgacggacag ttaccgcata
    15901 ttctgaaggt ttaggtttgt gttgtaaccc tttaaatgca gaatcaagac tatttttagc
    15961 attatttact tgggtttgag aagctttaga atcgtctagt acttttttag cattggataa
    16021 agcagtctga aaattattcc aagatccttc tgtaaaatct cctttagcag ttgattttac
    16081 ttggttgtac aaggcttgta attccgtttt gttcacagta ggagctggtt ttttctttaa
    16141 tcccttgtaa gcagaatcta atgtgttttt ggcactatcc acttgttttt gagtcgcttt
    16201 tgagtcattt aacacttttt tagcattgtt taaagcggtt tggaatgcgt tccaggtatt
    16261 gtcggtatag tcaccttttt tagttccctt aacgctcgta taaagagctt ctaaagctga
    16321 tttattcact ttgggtttag aggtgtcagc accataccaa ccaatacctt cgtatttcca
    16381 gccatagtct ttggttaacc attgtacttc tttccaactt gcagtgtagt gatgcgaacc
    16441 agaggtaaca cctggaagga aaaggcggta gatcggttta ttattttttt cggcagaacg
    16501 ccaggcaggc ccttcataag tccaaccgta atttttggtc aaccatttca cttcatccca
    16561 actagctgtg taatggtgat cacgaaggcc tttattgtat aaacgataga caaggtggcc
    16621 accgctagtc ggtgcttccc aagcggggcc ttcataatta ccccacttaa ttttcactag
    16681 gtgatcacgt tcttttgtgc tagaggtgta aaaatgttca tggttatttg gattataaag
    16741 gcgatacatg acgttagttg aagcagcgtc agcagatcca gcaagagcaa ctgtggcagc
    16801 aaatgttcca atagctataa cagtttttgg gtttaataat ttcatgtttt caactcccta
    16861 ttaaatgatt aaatattttg tttacattca taatatacca cggtataccg aggtagtcaa
    16921 cggtataccg tggaaatttt gaaataaaaa agccgcccta tgaagagtgt aaggatggct
    16981 gtttatatgc tatgaagaaa tggattcggc gtatttggtt agtttggcag cattatcaaa
    17041 tcgcattttt tcaatcgcac tttttttctt tattaaatcc gcaattgttg attctgcgac
    17101 tgaagaatgt ttgctaattt catatgctgt cttgccagtt tcaaatagta accattcgat
    17161 tttctcttta tcagcgtaca ttgcaatgct ccctcttgat tttttcttta ttatagctct
    17221 tttttgtaat aaatcacaat atcaagagga aaagtttcta tagaaaggtg ggaaattatg
    17281 agtatccgtt ccccgtgtat caaaatgagg tttgttgcta ctttcgttat ttaatatact
    17341 aaaataaaac aacgaaagaa ggaatagaat gaaaaagtta ataatggtat ttacttcgtt
    17401 gattgccatg attgttattt taggtggatg tggtagtgaa aaaaataagc aagatagtca
    17461 agataatggc gtgttaaaac caggaacagt gtggaaagaa gaagtaggcg gacttgttca
    17521 taatttaaaa attatagatg atacaacatg ggaatattcc gaaagtgttt ggcatcctga
    17581 acaagtacaa ataacagttg agaagctaaa agactataaa ggattagaaa gatataaggt
    17641 aatcgattca ggtggagtag atttattcat aggtaaaaca gccttaatag ttccctataa
    17701 caaagacggt ttagaaacgt ttaaatttat tccaacaaac gaaaaagaac atcgagataa
    17761 aagtgaaata ttaaaagaat acagtactaa atctggatac aagttacaaa agactagcga
    17821 gtgattgcta gtctttttat ttatcctgaa ggaggaatcg cagtggcaaa agctaaaacg
    17881 ccatttgaaa aagccgcgtc taaaatctta gaacgtcgcg gtgtatctga aacacagtgg
    17941 aaacaagaag tccttgaaaa agcacaattt gacctattac ggggaaaaga caaggactta
    18001 gaaggatata tctatcaacg tgaacgtgaa aagcttgttt tcgatgaaat tattaaacac
    18061 taaaaggagc gaacaatttt atgtttgaat caattacaat gaatttttta acacaaccga
    18121 aagtagtagc tgcggttgat atttttagag cattacaagg tgcagcaact agtttaaaaa
    18181 acaatctgat gatttttgcg gctgtagctg ccgttgcctc cattgttctt ggggggattt
    18241 tcttcttctt tggccgaaga ggagctgaaa acgggaagat gcacgtaggt tatgtattga
    18301 tgggggtagg agttatatta gctgccgcct ctattgtggt ttcgttattt ggaatgtttg
    18361 gacaaacccc acagggacta taaaaaataa atagaaggag taaaaagtac gtggctactt
    18421 tttactcctt ttttatgaaa ggagaatact catgtacttt acgatttcag gcaataccaa
    18481 aaaagataaa gagttaataa aaaaagcaac gaagaaagtg ggaaagcgta ctgccttttt
    18541 accgaaaaca attaaatatt ctgtcattga aacggaagaa cagctacatg tgttaggaca
    18601 agtgttagaa caagagaatc aatcttctgt taaattgact ctcacgttac atgataaaga
    18661 aggaaaaata gaaaaggctg gcacagaata cgtttttggc ccactggatt atcaaatttc
    18721 agaaggtttt tatcctacat tgatggattt gatccgcctt gatgtgttta aggaaacgga
    18781 tgaaaccatg agtgtggaag agtcttatcg aaacgaaaaa ctgctacaag ttttagaaga
    18841 agagttagag agcgaccaaa gaaaagtgag tcaagtgagt gaagtcgaag taaatgatga
    18901 agtaccatgg gaacgaccaa tctttccaaa gaaggagtca aaagaaaaag agacagtcta
    18961 tgttgacgaa caagctgata ttccgactca gaaatcgata tttgaggaag agaacgatgt
    19021 aatgggtcaa ttggaggtag atccctttga agaaacaatg gaggatgaag agacggccga
    19081 agtagaagtt gatctagttg aagctaatga agagccaaca tggactgaag gggattctca
    19141 ggaatatatt gaaccactta tcagtgaaga agtagagaaa aacgaaacag agatacaaaa
    19201 ggcaactgta tttgattata ttttggatca atatccgcca gagcatgaat ggatgaaaga
    19261 aaaaaataaa ttatttttaa atgagcttta ttcgaaatat caattacccc aaacctatca
    19321 agcattttta gagagtaaag aagaattaat ctcacagggt aaaggcacac ttttagaaca
    19381 gttagagtct gttcaatcaa caaattggca tgagcttgca caagaagatt tattagccac
    19441 ttttgaagaa cgtcaacaag aaacagatca agaaataaat gagtatgcga cggaacagtc
    19501 atctaattgg gagagaacaa aagaggaact aaataaagaa gaacaacgag tgattgaaga
    19561 agaagtacaa aaaattaaag agcgatacga aaagaaacgc gacaatgcgt attccttgtg
    19621 tatagagaaa attaataact ttactaaaac taatcaagaa gctttatcga aagaaaaaga
    19681 aaacttgtta aaagaacgta taaaagaacg aaaagaagag ttctaccgag gtttacgtac
    19741 agataagcta aagattagca aagaattaaa tgaacaactg aataaacttt atcaaacaac
    19801 gactgaagtt cttatggaaa gacacgggca gatccagcaa gaattacaag cacaaattcc
    19861 aaaatggaaa aaagagtatg aaattcaaca acaagaattg aaagaacaag aagaaaaaca
    19921 acgacaagaa gaaaaagagc gcgaacaatt agaactaaaa aaacgtgagc agcatcttgc
    19981 agaacaaaaa gtggcgatag aaaaacaacg gttagagcaa gaaaaggaaa aagaagcagc
    20041 tgctcgtaag gaacgtcaag aacaaatgga tacattgcga caagcacagt tgaattatct
    20101 agcacaacct caaccagtag ctgctgttca aacgccagtg aacaaccaag caaatttaac
    20161 tccttcacca cagcgttcct ttaacggatg gatggtggga tgtattgcaa gtttaagctt
    20221 gcttctaggt ggaggaacag tatttgcttt taatcatatc aatcaatctt cacaggcaga
    20281 ttctgtagcc acaacggcgt caatcaagga agaagcccag gaacaagcaa gaaaagagta
    20341 tgaacaacaa ttggaagaat tattgaatcg taccaatcag tcaacagcag agtcaagtat
    20401 ggaaaatagc aaagagagtg atcctagtaa ggctaaagaa ggatcaagcg attcaactgt
    20461 acactcaaac acttccgaaa gttcagaatc aaatgaggtg aaataatgcg tattagagaa
    20521 acaacgagac gaaaagacaa acgattcaat ttagaaaaga ataggtatct ttctcatttc
    20581 tatcgctata ctccttcagg agaagagcta ccagaaaaac atgacaaacg tatttctcaa
    20641 aagatttttt atagtggctt agcttatctt cctttaaact atgttgttac aagaatacca
    20701 gaagcatttc atcgatttga gattattcca ggaagtttac aaggagtgaa tatttttcag
    20761 ttacaagaaa tgccgcagtt attccatttt gattcaactg gtttttggaa tcctttgacc
    20821 ccaacaacaa gtttaggcac acgtttagtt gtttctacga tttgtgttgt tccagctgta
    20881 cttatgggat tgaaaatgga catgtggttt cgtccgctag cagatgggca aaaaggagat
    20941 aaccgattaa aaacgttaaa ggaaatccaa caaatttatc cgcaaatacc agaatcaaag
    21001 ggaagttttc ctggttatgg cggaatacca attactcatt ataaaaaata ctggtatatt
    21061 cagacggata cagtaaatac ttgtattgtt ggtacttccc gctcagggaa aggccagata
    21121 gaagttttag caaccattga caatttgtcg cgtgcagaaa gacaaagttc aatggttgtt
    21181 aatgatccaa aaacagaatt atatgttgct agtaaagatg aactagaagc tagagggtat
    21241 gatgtgtatg cctttaatat caccgatcca ctgcaatcaa tgagtgataa tccattggca
    21301 ctcattgtga aatactggaa gcgaggagac atagacaccg caacacagtt aaccaatact
    21361 tttacgaata cgatttatta tgatgcaaat gcttcagaca ataaatactt taacgaaaat
    21421 gcgcagaaag ctgtcaatgc gcttattttt gcgttattgg aacaagcgga tcaaacaggg
    21481 gattactcca aattaacacc aaataatctc gttgaactac tttcagaaat tggcggtttt
    21541 aattattatc cagatcctga gaatgaattc aagcagctca atgccttaga tgagttcatg
    21601 aatcagttac cccctggaaa tatagcaaaa aaacaatatg gggcaacaaa aattggatct
    21661 gataaagcga aagggaatat cttgtctacg gcaattacag gattgaatcc atttacccta
    21721 acgaaaattg cgaagatgac ttctcaatcg accattgatt acaagtccat tggttttccg
    21781 aagtatttgg atctcaaatt acatgaaagc ctattaaatc aacgcattgt cgtggaattt
    21841 tatcataagg gaaagaaatt tcacgaggaa cgagtcaaag ttggctatcg tggtttttgt
    21901 gaaatcaact ttgattgtca tttagagcag ggagatcatg tttgcttaaa atacaatcat
    21961 caaggaaaac aatatgttgc ttggtatgag ttttctcaaa aagtattgaa agacgaagag
    22021 ggaaaaatcg tttatcgaaa aaaacgagga gaaactcatt tgccagaact agaaaaagaa
    22081 gccgttctaa cattagatga gaagaaaagt aacctattag tagagtctat tcggatgcac
    22141 tataccgata aaccaatggc tatttttatg ttaacgcctg attatgatcc atctaaccat
    22201 atcattgtta gcatctttct tagtcagttg tataaagaat tatctacgca atgtgttaag
    22261 actaaaggag ataaatgtca ccgtaggatt cattttattt tagatgaatt tggaaatatg
    22321 ccaccgatgg acaacatgga aggaatcatg acggttacgg caggtcgtaa tatgttatgg
    22381 gatctgttta ttcaatctta tcagcaactt tatgcgaagt atggagaaca aaacgcagcg
    22441 attatcaaag aaaattgtca gacacatatt tatattatgt cgacagatga taataccatc
    22501 gaagaatttt ctaaaaaagt ggggaataag acggttgaac aagagaacag taccatcaat
    22561 tcattaggta tgaatactca tatcaatcgc aatgttgatt cagatcgtat cttaactcct
    22621 gaaagaattt ctactttttt agaaggtgaa acgattgtac tcgctccttt aaaacgacaa
    22681 gatttgaaag gtcataaaat acggccgtat cctattttta atcatgggga aacgaatctt
    22741 ccttatgctt atcaatatat tgaagatttc aatccatcgt gggatatcaa tgatatagac
    22801 atacattgta cacatgcgga cttggatctt aaggctttac aaattgatta tgattatttc
    22861 ctaccagatg aatatgctag aaatctctat catggactgc atgatcaatt agaagaaaaa
    22921 aacgaagaaa atatgtttta taaaatcgtt caatcttttg agaatgagac ggctaaaaaa
    22981 ttgttaattc aattttacga agaacaagca acggatcgtc ttcaagaata tgttgaaaag
    23041 ttgatcaaaa atgaagaaat ttctgaaatg attaggaaac ggctgttatt ggaagctgaa
    23101 aaggcggtga aagaatgatc attggaggaa ttttctctgg catagccgac tggtggtcag
    23161 gtttattcgg agaaggggat gcagatccca aaaccgtaac gaaagtctta caagattatg
    23221 gtaattatct gcagtatcaa gatgtattca gttggatttg taatactttt ttatggggat
    23281 tgatcaaagg gttatataat ctcaatactt tattagaaaa aacaatttat caaagctttg
    23341 atttaaaaga cttattgaac gccgctggtg ttaatgaact atttcaaagt ttgattagta
    23401 aggtattggc tcttttttgt gtcgtcacat taatttattt aggcttgaaa ttttttatga
    23461 gtaaacaccc accgaaagtc aaaaatatct tggttaattt atttatggcc atgatcttga
    23521 tttttggtgg tagttcttta atcgatgaag ggttaaatat cagtaaaaac ttttatggag
    23581 acgtcactgc agcaaataaa gcagataaaa cgggttcacc tgcttttcaa attattcaaa
    23641 ataatgtgat tgatgtcagt acattactag cgactgaccc gaataaagta gaatccttac
    23701 caacagataa aagaaatgct ttaacagcga aaaatttcaa aacagcgaat atcaatggga
    23761 ttattacacc tgatcaagct aagaaaatgt ctgaatcaag tgatattagt acagtacaaa
    23821 aagatcggtt aaaggcatta cagtatcgac tagagttaga cgataatggg aaagaaatcc
    23881 ctgttcaaat tagtgatgaa ggtttagcaa aatatgtgta tacgtcaggc tatcgtcgat
    23941 acattagtag tccagggatt attttggctg gggaatcttc tttgtcggta gcctatttat
    24001 ttattttgtt tacaatcgtt acttgcatta ttgagctagt atttaaaaaa ttctatttag
    24061 taattgccgc gtcaacggac tttgagacag gacaacggat gaaaagtgcg atagaagata
    24121 tttctcaatc cttcttgtta ttggcgttta cgattttaga attacgaatc tatacattaa
    24181 tgctttcagg tatcggtgat ttgcatgcgg taggaaagat caatggattc ttgtatgttg
    24241 ttgctttaat tgtgctaact attgcgctat tcaaaggctc tcaatcggtt acgaagattt
    24301 tcggtgttga tacgagtctg aaaaacggca gcaattctct tcttagcatg tttgccttag
    24361 gaaatattgc gaaaaatgtg ggtagcggag ctaaaaacct tgcaagtgct ggtaagagtg
    24421 gtttaagcca tttaaatgat attcgtaaga atggatttgg ccatcgaaaa gatattaccc
    24481 atgataacgg ggaagaacaa tcagacgata aagctacaac tgtacctaat agtaagaaga
    24541 cagtttccga aatgttcggg aaagcgaaaa atggtgtttc taaagccgca gaaggtcttg
    24601 gttacgtcaa tgaacgtggg ttaaaaggaa cagttgaaga tcttacagaa aaagccaaag
    24661 acaccttaga agagaaatta gacggcacga gagctaaaga atttgctgaa gctgtgaaga
    24721 atccatccga agcactagaa aaacaaaaag aaaaagcttc agcagtcaaa gaaggactaa
    24781 aagatgcgta tggtgaagga caagtcaatg cagcagtgaa ggcaaataca acaagtgaaa
    24841 aagaaaatca agaaggtgca gcgattcctt cagccaaaga tcaccaagat gagttacatt
    24901 ctaacgaaaa ttcgatcgga gaaaaagatt cagaagaagg acaacatggt cagttaccaa
    24961 aaaccgatcc agaacaatat cgttataaat ctaacattaa ttctgaaaac gaaaaacgca
    25021 tttcagattt accaaaacga aatcaagaag tacaaacgga tgaggattcc atcgatctgc
    25081 caaatgataa tggcatcgaa gttggaactg aaaaatcaac gaaaattggt ttgcctaaga
    25141 cacaaaattt accaatgaaa gatagtcgtt tgaatcaatt caaagagaat gatcaaagct
    25201 ttgttcatca atcacctgca ggtactgttc aaatgaaatc aagtcgtttg cctgaaaatg
    25261 gaaatctgac aactgtttct tacaatttag ctgaaagtgg tgaaacagag aatgcgtccg
    25321 ttcaaaatat tgtgacaaaa gaagctgcac ctaagccaat caataaagtt cataatggat
    25381 acagaccagc aaacgtagca tttactgcac cgacaaaaat aaatgtgaca agagaaacta
    25441 aaaacaggaa caatgaattg aaagaacaac tattgaagga tatcaaacca taaaaggcct
    25501 atttaagggc ctttttcttt ataaaacaag tttaaaatgg aaaggagaaa attgtggagg
    25561 atattttaat agctacgttt ataactgcac tgggagcatt tttaacgata tttgtggtga
    25621 ctattaaagg atttaaagat acggatagtt tacaaaaaga tcatcagtct attttagatg
    25681 gtcaaaaaac acataccgaa agcattattg tacacctttc agaaggattc acttatttga
    25741 aagatgctat gcccacattc acagtaaaat taacggaaat atttcattct gttgagcaaa
    25801 tatcgtcaga attgaaagag caaagacaac aaaaagatcg tcaacttggc tcacttaatt
    25861 ttaggcaaga aacgatacaa gaatcattgg agaatgtaaa tcgattaaac gatgaaatgc
    25921 aacgagttca tttggaaaat gaagaattac gcgcacaaaa ttatgattta attcaaaaga
    25981 aaagaaatct ggaacaaaaa attgttgaat taaaagaaga aaatcgtcaa ttgaattatc
    26041 aattacgacg aggaaaaaat cgttctcgtg atttaggaaa tgaattagag ttgtaatatt
    26101 tagaaaaacg ctgtgagaaa tcacagtgtt ttttatttct aaaaatacaa aatataaggg
    26161 gtatggttat attatggata aatacattca tatgaaaaat cctccaagat ttagtgggga
    26221 atggaagttt ttcgcctttt tctttttaga tctattaacg cttggtggct gctttttttg
    26281 ggcgaaaata gtcaataatc ttgtccactt accactcttt tttgcactct tgaattatgg
    26341 attatggctt gcgatagcgt tagtgattgt ttggcgaccc tataataacc ctggtcaacg
    26401 ccaaattttt gcgattatcg aatctatttt ctttacggat aagaaccact atcatgcgtt
    26461 agatcctaac cgaaattatc atggtacgta taaggaggat gaataaatgc gagtcaaagg
    26521 acaaaattca gttgataaga aatccatttt gcagtttgga aaaccagaag agcgctctat
    26581 cttggactta tttccatata aatcctgtac agaagaggga gtcttaatta ctagagaaga
    26641 tcattttcag cgttattatc gtgtcgtttc cactgacgtg gagggattaa atgaacaaga
    26701 gaaactagaa cgaatgaatc agttgaccac cgtcatgcgt acgtatgtgc caacaattaa
    26761 gttagttagt ttgaccacag agacggatct aacggaacaa atcgtgcaaa agcggcagtt
    26821 attagataaa aatcgtatgg aacagtcact aggaagaaat ctacagcgat taagaaaata
    26881 tgaaaagaaa ttagtagagg agatccagga attaaaacgg gcagaacaag aacgtcctga
    26941 cttaagcttc tttttcgtta ttgaaggaaa gacaatcaag gatttacaaa ctagaaatcg
    27001 tcagttaatg cgatctagtg gcattttagg gttaaaacca ctctacaaaa aagaattgat
    27061 taagattctt tatcgaatga acaacatgaa tgacgaataa aaaagatgga ggaaatgatt
    27121 catgctagat gtgataaaaa cagctttttc aacgcaaagt aatcaaaagg tgattgatcc
    27181 attaaaacaa cttggtaatc gtgaaataga gattttaaaa tcaaaaggtt atgatttaga
    27241 tttcttaaaa caagtgatgc cacaaggtgg gttgaatttt gatgaagaat atgtgaccta
    27301 tggaaatggt tattcacgat gtgtgacagt ttatcattac acaaaaaatc ctactctttt
    27361 ttggttggcc aatctcatga acaatcctca tacagtggcc acactagatg ttagcacaga
    27421 tgaaaagcaa aaagtgattg atcaaatcaa ccgcgcatta gatgaattaa atgatcgatc
    27481 agataatgaa cgaaaagcga ctgaccgaaa taaaagtgcg gaagaatacc tggaactagc
    27541 ggaatatgcc cgaaaattga cttctaatgg ggaaatttct aaacaagtca aagtgagagt
    27601 acatgtttac ggaaatacga tccaagaagt ggatgaacgg gcgcaggaac tgatcgaaaa
    27661 tctaaaaggg atggattatc gtgcggtgac ttatctgcat aaaaccaaac gagattggca
    27721 atcgatgttt accatgttcg atgaacaagc tgaatggttt ggaaatatca aagggcaatc
    27781 acttccatcg ttaaacattg gcggaggatt cccgttccac caccaaacgc taaaagatcc
    27841 aggaggcaca caatatgggg aaaccatgac aggcggccca tttgtctgga atgccacgta
    27901 taaaaatagt gtacgtttat ctaccaatac cttgcttcta ggaatgatgg gagctggtaa
    27961 atcgaccgtt cttaaaatga tcgcggaagc ccatttagct gcaggagact ttatttgggg
    28021 ctttgaaaag ggaaaagact ttattccttt cttaaaggaa tataacggta ttatggtgcg
    28081 tttagacgga tctgacggga tgatcaatcc tttagagatc tttgccacac ggacgtatga
    28141 tgaagcatcc agtttatatg acgatggttc agtcaaagat ttgaagatca atgaagcagc
    28201 gtcctatcaa acgcacttag acaaagtagt ctatcaggta cagttagtga gtcctcagct
    28261 aaaaggaacg atgaaagcag aattcaagac ctacttgaat cgtttttatg aagagtatgg
    28321 aacagtacca cgcggtttta cttcctcaaa ttcgcgttca aatactgata cgcaagtcac
    28381 agggaaagat ccagaagctt atccgacgtt caaagaattt ttagcctttt taggacaact
    28441 ggaactacca ggggcttccc aagagaaaaa gaatcggaaa gaagaaatgg agtctatcgt
    28501 ggaatcctta tgtgagactt atggcatgat ttttgatggt catagtacga ttcgacacct
    28561 tgaccaacaa cagttggtct tttttgacat tgactctatt acaggcctag atcgaggggt
    28621 acaacaatgt cagatgtaca tggccatgac gttgatttgg aatcaggcat tgattcaagg
    28681 acgaagacaa cgacgcttaa ttaaagaaaa acaaatggaa gaacaagact tcaaacgctt
    28741 cttggcgatg attgatgagt gccataatgc gattaatcca gatttcattg aaacggtaaa
    28801 ttatgtcaca aacttccaac gagaaatgcg taaagtagaa gcaatgactg ttttagctac
    28861 acaatcccca caagaaatgg tgccagaaaa tatgtcttct gataacttct cgaaactgaa
    28921 gaaagtcttt gaattttctt ctacgaagat ctttatgcgt atggatgaat ctattttacg
    28981 tcatatagaa ggattagttg gtaaatcaat gacgaactca gaattagaat ccattcctca
    29041 acaaaatcaa ggagacgctg tgatcaattt tggctcaaaa gaaaccattc gtgttcattt
    29101 tagtccgaac aaacgtcaat taaaattgtt tgatggagga aaataatgga aaaccaaaat
    29161 gaaagtttaa tcaaacagta tgttaagcgt cgtgcaaagc ggcgcttatt tttgtgggtg
    29221 tttggtactt ctgcaggttt gattactata ttgatcactg tgtttgtgac cctttttcta
    29281 atacttgcgg caggttctat tgataattct gattcaagta gtggtggcga agcttttaca
    29341 ggagaatatt cggaaggctt acccatttat aaagaaatca aaggaagagg ccctttttct
    29401 gatgagattg cgcaatatgc tgtaggtgca gcagtgaagt ataaattatt accaagtgtg
    29461 atcctttcgc aatacggtta tgaatcagcg tttggtacgt cggcgtccgc aagaaatgat
    29521 ctcaattatt ttgggattac gtggtttgat gggtgtctgt ttcctaaagg aactgcgcgt
    29581 ggaattggtg gcattgaagg tggctggtac atgaagtttc ccaactcaaa agctgccttc
    29641 tcctattacg gctttatggt agccacacaa tcaaatttta atgcttgtgt cggaaataaa
    29701 agccctggcg caagcttatt gatcttagga cgaggagggt atgccgccgc aggaattact
    29761 gaagacagtg cctattataa aaattgtatg tctattatta atacaaataa attgatggag
    29821 tacgatgagt ttgcgattaa acactggaga gaaggcggcg gaactggtgg tacgattaca
    29881 ggaagctggg gcaatccatt cccaggaagt agcttagata aaaattcgtt cagtggagga
    29941 caactttttg ggaaaaatcc aggaggagaa ttccgaccta acgggttcca tgatggacta
    30001 gactttggtt cagtcgatca tccaggaagt gagattcatg cggttcatgg cggaaaagtc
    30061 gtgtacgttg gcaatcctgg tatttcaggt ttgggtgcgt gtgtgatcgt gattaattac
    30121 gatggcttga atatggtgta tcaagaattt gcaaatagta caggaaattc tcgagtgaaa
    30181 gtaggcgatc aagtgaaagt tggtcaagta attgggatac gagatacagc acacttgcat
    30241 ttaggattta ctcgaatgga ttggcgtcaa gcacaaggac atgcattcat tgatgatggt
    30301 acatggatcg atccgttacc atttttgaat agcagcaaaa aatagatcag gagaaaaaat
    30361 atggaaaata aaaccttaaa aatcgtctta acagctgcaa ttagcgttag tttaatcggc
    30421 aatattgcgt tgttttggca gcttaaagaa gaacaagaga caaataccta taaaattgaa
    30481 gaagtggcta aaaacgcgtc tgataagctc aaaaaaaatt cttctgaagc acaggcgaag
    30541 attgacaagt taaatcaaac catcaatgag aaaaaccaag aaatcgatca gctgaagcaa
    30601 caagatggca caaaagcaaa agaggaagta actgaagcac aaagaaagac ggcagaagag
    30661 ttcggtcaat tggccattga taaatcatta agcagaaatg aattaacgga aaaactaaaa
    30721 gatgtcgcaa cccaagaagt gatcgacaaa ttaagtcctg tagacgatga tcaccaacat
    30781 gatgattacg gaagttactc ttttacatta ggtgacgtgc aaacttacgc tcaaacaagc
    30841 agtgtaaaag aggaacaaaa ttttgtcgta tttgttgatt atacaatcgg caatcctcaa
    30901 ttcaaagatg tcaaaccaca aaaaataaaa ggtggattaa ctgtcactga aaaacaagta
    30961 gatggtcaat ggaaagtcac agactttagt tatttcactc gttaggagga agaaaatgag
    31021 taagaaaaaa ggagcaagac aacgttttaa aaaacgaaaa agttatctta gatggctggg
    31081 gatctttatg attctcggct ttttgattct tgctagtccg atttacacac ataaaaaggt
    31141 agacatacca gatgaagtaa gcacgaacgc atttttaaac acgaaggaac tgacgatggt
    31201 ttcaaaagaa aaaggagcga accagcaatt agtttttgaa tttgcgatcg acgatgaaga
    31261 tcagagctta ttaaaagaac tagctaatct taattatcgt gtagaagtaa agacagctaa
    31321 aggggattat caagcaataa aagccaaagt gatcaaggtg tctgatgatt attttgtagt
    31381 gaagttagca catgtaccag aagaatatat tgctatgcga ttaactatta ttcctgaaaa
    31441 aattgatcca aaggtggata tgcaagaacc gcaagatctg attttttata ttcacgaaga
    31501 taaggtcaaa gatcacgtga aagacgaaga ttatgaacaa catgcgatca attacaaagt
    31561 gaaaggatac aagaaagaac aaaaagcagc acaaaaacaa attgaatcct tccaagcaac
    31621 cattgatttg aatgaagaat tagtgaagaa actaaaagat caattgcctt atcaagtcgc
    31681 agaagatcag gaaaatactg aaaataagat taatagttac cagcaagaaa ttgaaacatc
    31741 gaaaacgcaa atgaaagatc aagaagagag cattcaaaaa cttcaagaaa aaattgatcg
    31801 tatgacaaaa gaaaatattt agaagaatag gtggagtaaa agaaatgaag cgagcgaact
    31861 tggtcggcaa agatactttc gctgagcaga aagtattgat taaagcctat ctcaaagttg
    31921 taaaagctaa agaaatatgt caagaagtga attatctaac acaacaatat ctgagttgaa
    31981 gaatggtaga aagagtttag ataccttaaa attcagatca tttgaagctc tatatatagc
    32041 tgctaaaaaa tattttacag ctgataataa ataaaaaatt aaggtgggtt ctatgtgaaa
    32101 aaaacgtatc aaaagttttt atggattagc tatatcgtcc tatttatcgt tgggtttctc
    32161 gtcagtgtgt ccgtgatcgt cggaacgctt tttcttttgt tctctgacga agatctggta
    32221 cttaaggggt taatgagtac gttatcgtgt gtcatgttac tttatttatg tatacagatc
    32281 aacgaaatta tgccccctca ttggcaagaa aagaagaaag aatttaagga agaaatcgaa
    32341 ataaaatcca atgactggat tgaatacaca tggcttcctc tgaaaagaaa tagcaaacga
    32401 aaggaatttt gggcattgca attatggcca ataggaaaac tttttattta tatggcttca
    32461 cttatggtta ctttaatcgg ttatttggtg gtggtctatg gggtggccta ttacttgtta
    32521 agtgagccaa aacagatcgc ggcgtcaaat attttcacgg cagtaatgac attaatgtgt
    32581 gtcatgtgct tttctatttt tagttactta ctcgtccaat ttatcaggca aaatttaaca
    32641 atgagtaaac ttttcaaaag tttattggac ttacttatct taggcagtat gttaacgact
    32701 ctaacagatc ggttcgaaca atctgaaaaa acggtagagg ggataaagaa cttcttaacg
    32761 acgattgatc aatttataaa tggatatatg tatttgtttg aagtaggctt tattctcact
    32821 ttgtttggtt gtgtatttat aaaactcatt aaaagatcaa tcgaaaaaga aatggttatt
    32881 gatctaattc cgacgctgag cggctatgag tattacgttg attttggaaa gtatgaggag
    32941 ttgatccata cggatatcgt tcacacgagt atccatgaag taaccgttaa gggcatcaga
    33001 tctactttaa aagatggaca ggagacgaaa acaatcgacc gaagaaaaga ggtctacaat
    33061 ttttataaag ttgaagcaag tctacgatat ggcattggga aattgaggtt agatgggagt
    33121 aatattcaac aaaacaaacc atatgtttta ggaaaagtgg tgatttagaa cttcctatga
    33181 aagatgaata aataaggaga atcttttgaa aatgattctt ctttacagaa ttgtgcttat
    33241 agttggcacg gagttaaaaa tcttaaagaa ggagcagtta ttcaaagtga aagaacatca
    33301 taatcagtta ctatgtatta aaacgggtac gatgaacatt caataccgaa cagaatattt
    33361 agaattaacg aatcaaactg attttttgtc gattcgagag caacataagc aagaaaaatt
    33421 aatggctact tatagccaat tgattgatga gtgggaacaa ttcaaggaat ctaaaacata
    33481 aaattccatt taaacacctt attttttaaa gagcataagg aaacgtgtac ccactttcaa
    33541 aataaagtgg gtagataggc tgtgcctatc acgtttctga aaaaaataaa tgaccccaaa
    33601 attttcaatt tatgtgtagg tttatgggta gcaaaaatat gaatgtgggc agcaaaaagt
    33661 tcatttgtgg gtagtaaaat tcaaatgtgt gtagcaatgt gtgtagctac acacattgct
    33721 acacacgcta taacatatag tgaaaaggag atatttatgg aagtaaaaat aagagatatt
    33781 gatgaaaaaa cgattcaaag aattgaccga agatgtattg aattaagtga acgcaccggg
    33841 actaaatgga gccgaaatcg atacctaaaa gttttgattg aaaatgattt tgaccatgaa
    33901 cttatggatc atagaaaaga tcaatttgat cggttgttag aaaaatttgt ggatattcaa
    33961 acttataata cgaaaatttt agaagaatat attgcaacaa ataatcaatt aatcgccttg
    34021 ttaattgaat aataaaaata gtattgtgaa tttataaaag gaggaaatca attttggcaa
    34081 aagcaacaag aaaacatgta tatctttacg atatgcatat cgaagtactt gatgaaataa
    34141 tttctactca tgaagatata tctaattata gtgaagcagt cagacatttg tgtatgatgt
    34201 atgatccaaa aatcgaagaa aaaataaatc caattcaaat taaattgaat gcaatgggaa
    34261 aagaaatttc tatattaacc gaaatggttt cagagtttgc ggatcttcat ttgaaagata
    34321 caggaatact aattggtaca aattcaactg tctatcaaga agcaaaagag cgtgtggaag
    34381 ggaaaataaa agcgaatcaa acgaaaaagt attctcctaa gatcaagaaa ccaataaatg
    34441 aatctgttca tgccagagta gacccgtttg atccaacaaa gtttttaaaa agataaaaag
    34501 tagaagggga acatgatgaa taaatcgcct gcagtcattc tcacttgtca atttaccttg
    34561 cccaataaaa aatctttttc aacatacatt gattatatga caagagaaaa agcgctggaa
    34621 gaaaaggaac gacgcacgcc ggacgaagaa caggaactgc aacgaatcaa gaatgcttta
    34681 gaggattttt atattcctga aggagaaact ttttcggaga gtaagacaga taaagaattc
    34741 agtgaaacgt cgaatgaagc tcaatcgttg atggaacatg gaatcaattt tgacaagtta
    34801 gaggaacaag attttaccaa atatctttct tatatgaccc gtcactatgc gttagaacaa
    34861 aagaaagaac tgacgcaaag ggaacaaaaa gaataccaac ggttgaacca agcaatgaaa
    34921 aaatatcaaa cagagccaca taaaaaagct tcaggaaatc ttcctggagt tttttctatg
    34981 gcttcagacg aagtgaaagg aagtgatctg aaggagatca aacaaatctt ccaaagaggg
    35041 caacaaaaag gctcaatcat ttatcaagat gttgtctctc acgacaattc gtatcttgaa
    35101 aaaatgggct tgtacgatcc caaaacagat accttagatg aagaaggatt aaaagcagct
    35161 ggaagaaaaa tgatggagac attattcgag aaagaacaat tgaatgaaac aggttactgg
    35221 atggcaacga ttcaccgtaa cactaagcat atccatattc attttgcagt tgttgaaaaa
    35281 gaaaatacgc gtaaacctta cacggaggtt gaaaatggtg tgagttatat cgtaccgaaa
    35341 ggtaagagaa gacaagcaac attagacgca atgaagacca attatgtaca tgagttagaa
    35401 cgtttttctt atgaaaagga tcgaatgatt ggtatggaga aacgaaattt attgacgcgg
    35461 aaaagtgatt tgcgtaacac cttaacaaaa gtggtaaaag agccagcgcg ttacgatcga
    35521 cacgcaatga acttattaaa tgaagtatat aaaaccctac ctgacaaacg ttcggaatgg
    35581 cattatgggg atgaaaaacg aaccaaactt tcaccataca caagagagaa actcgatgag
    35641 ttaaccaaat atgtgttaac aaaagaacct gaatatcaag aatatataca gatttcaaaa
    35701 tcattgaaag aagaagcaaa aagtctttat ggggaaagca agagagaatc aaaggatgca
    35761 gaaaaaaatg ctctctttga tttgaagaag cgcacaggaa atgccattct aacagaactg
    35821 aagaaacagg atagtcaaat aaaaccttta atagaaagta tggatcagat gcaatctatt
    35881 tacaatgaac gatcctttcg aacgcttaaa cagttaccaa cgaatgaata caaatatagt
    35941 tacgaagaat tcatgaagcg caggaaacaa ttccaacaaa gagttgtcac gcaacggagt
    36001 ttgcgtcaat taaatcgaac gattaatgcc catgaagaaa aataccacgc aagtaaagcc
    36061 tacgaagagc aacaacgaag aattcaaatg gaacaagaaa gacagtaaag acagtaggag
    36121 gaatagcaat gaaacaatat ttagatcaat ggaaagttat agaaggatca ttaagagaag
    36181 aacgaatcga acaacttccc gattgtttag aaaaagagca tttgtttcag ataagagaaa
    36241 tgttgagaaa cgaacagttt gatccaaatc aatttttagt cgttgagtac tcagctactg
    36301 gtgtgtactg ttgtaatcat gtgaagggtg aaaaatattt tatcattcaa gaatatgagg
    36361 gcaagttagc gccttattat actacttggg aaatgaatga agaggggatc aataacttcc
    36421 cgtgtaaaag cattgaagag tctatttcac tcacagaatg ttagttgtta agtgaataaa
    36481 agaagtaaaa gagtcctgaa cgtaattgtc caggactctt tttttcaatg aattgttttc
    36541 cgccaattca atgagtggtg cgatttgcga ctacattata tcaaaagaaa gagaagggag
    36601 gaagaagaaa tggcttattc aacgattgga gttgttgttg aaaaaagcag agacaatctc
    36661 gtttttgtga cagaaattca aacgggcaga gcttttgtcg tcacagacaa agcggcaaaa
    36721 gcgtatcaaa gtggcgatat tttaacgtta aatctgacta cgaagacgtt cgtcgatgcg
    36781 gcagaagatt atcc
//
LOCUS       DACCCS010000021        36582 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.021, whole genome shotgun sequence.
ACCESSION   DACCCS010000021 DACCCS010000000
VERSION     DACCCS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.021"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(438..611)
                     /locus_tag="IVK82_001142"
     CDS             complement(438..611)
                     /locus_tag="IVK82_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HAP6392216.1"
                     /translation="MNRYVHGTVQKKNRKINGKLLVNPNGYEWLRAKGSYPVPKYCNY
                     SETLMEWYQLIIT"
     gene            complement(954..1643)
                     /locus_tag="IVK82_001143"
     CDS             complement(954..1643)
                     /locus_tag="IVK82_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP6392217.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFSHTRVGKNGKTFKMHKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYIGHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(1706..2470)
                     /locus_tag="IVK82_001144"
     CDS             complement(1706..2470)
                     /locus_tag="IVK82_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP6392218.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIVKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(2498..3196)
                     /locus_tag="IVK82_001145"
     CDS             complement(2498..3196)
                     /locus_tag="IVK82_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP6392219.1"
                     /translation="MARTQKQKAAQTHVVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTIAANIAVVFAKSGQRVLLVDADLRKPVVYETFQLNNAS
                     GLSTALSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYRN"
     gene            complement(3208..3987)
                     /locus_tag="IVK82_001146"
     CDS             complement(3208..3987)
                     /locus_tag="IVK82_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP6392220.1"
                     /translation="MEETISLKEIFGTLRKHLATIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSENSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4003..4947)
                     /locus_tag="IVK82_001147"
     CDS             complement(4003..4947)
                     /locus_tag="IVK82_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6392221.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4993..5772)
                     /locus_tag="IVK82_001148"
     CDS             complement(4993..5772)
                     /locus_tag="IVK82_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392222.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKERVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNINY"
     gene            complement(6157..8232)
                     /locus_tag="IVK82_001149"
     CDS             complement(6157..8232)
                     /locus_tag="IVK82_001149"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP6392223.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDYEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTYHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDADRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(8234..9151)
                     /gene="glyQ"
                     /locus_tag="IVK82_001150"
     CDS             complement(8234..9151)
                     /gene="glyQ"
                     /locus_tag="IVK82_001150"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP6392224.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(9537..10349)
                     /gene="recO"
                     /locus_tag="IVK82_001151"
     CDS             complement(9537..10349)
                     /gene="recO"
                     /locus_tag="IVK82_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP6392225.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKDMSEKDLPKKEE"
     gene            complement(10492..11391)
                     /locus_tag="IVK82_001152"
     CDS             complement(10492..11391)
                     /locus_tag="IVK82_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP6392226.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(11406..11807)
                     /locus_tag="IVK82_001153"
     CDS             complement(11406..11807)
                     /locus_tag="IVK82_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP6392227.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIVGLILFVPKIYHLLFN"
     gene            complement(11785..12261)
                     /gene="ybeY"
                     /locus_tag="IVK82_001154"
     CDS             complement(11785..12261)
                     /gene="ybeY"
                     /locus_tag="IVK82_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP6392228.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(12290..14482)
                     /locus_tag="IVK82_001155"
     CDS             complement(12290..14482)
                     /locus_tag="IVK82_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP6392229.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLFAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(14506..15477)
                     /locus_tag="IVK82_001156"
     CDS             complement(14506..15477)
                     /locus_tag="IVK82_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP6392230.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            16183..17562
                     /gene="brnQ"
                     /locus_tag="IVK82_001157"
     CDS             16183..17562
                     /gene="brnQ"
                     /locus_tag="IVK82_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP6392231.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGTNWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(17718..18164)
                     /locus_tag="IVK82_001158"
     CDS             complement(17718..18164)
                     /locus_tag="IVK82_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP6392232.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(18191..18367)
                     /locus_tag="IVK82_001159"
     CDS             complement(18191..18367)
                     /locus_tag="IVK82_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP6392233.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(18529..18957)
                     /locus_tag="IVK82_001160"
     CDS             complement(18529..18957)
                     /locus_tag="IVK82_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP6392234.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            19049..19909
                     /locus_tag="IVK82_001161"
     CDS             19049..19909
                     /locus_tag="IVK82_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP6392235.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(20004..20297)
                     /locus_tag="IVK82_001162"
     CDS             complement(20004..20297)
                     /locus_tag="IVK82_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP6392236.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(20370..22097)
                     /locus_tag="IVK82_001163"
     CDS             complement(20370..22097)
                     /locus_tag="IVK82_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP6392237.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            complement(22311..23237)
                     /gene="trxB"
                     /locus_tag="IVK82_001164"
     CDS             complement(22311..23237)
                     /gene="trxB"
                     /locus_tag="IVK82_001164"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP6392238.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(23382..24815)
                     /gene="ascB"
                     /locus_tag="IVK82_001165"
     CDS             complement(23382..24815)
                     /gene="ascB"
                     /locus_tag="IVK82_001165"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP6392239.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(24815..26218)
                     /locus_tag="IVK82_001166"
     CDS             complement(24815..26218)
                     /locus_tag="IVK82_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP6392240.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            26448..27350
                     /locus_tag="IVK82_001167"
     CDS             26448..27350
                     /locus_tag="IVK82_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6392241.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(27391..29277)
                     /locus_tag="IVK82_001168"
     CDS             complement(27391..29277)
                     /locus_tag="IVK82_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6392242.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIRAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILMMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGNLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKTSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFKSL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(29373..30224)
                     /locus_tag="IVK82_001169"
     CDS             complement(29373..30224)
                     /locus_tag="IVK82_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6392243.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSEQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGEKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(30681..32465)
                     /locus_tag="IVK82_001170"
     CDS             complement(30681..32465)
                     /locus_tag="IVK82_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6392244.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQTITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(32465..34216)
                     /locus_tag="IVK82_001171"
     CDS             complement(32465..34216)
                     /locus_tag="IVK82_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6392245.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFVIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(34358..34582)
                     /locus_tag="IVK82_001172"
     CDS             complement(34358..34582)
                     /locus_tag="IVK82_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP6392246.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            34833..36419
                     /locus_tag="IVK82_001173"
     CDS             34833..36419
                     /locus_tag="IVK82_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP6392247.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT        10137 a         7809 c         6451 g        12185 t
ORIGIN      
        1 tatcttaatt atctatccat ttcatcctac gcattcttgc gtaaatacaa ttcactctgt
       61 tgagctgata taaatcattg gtggtacaaa gataaaatga taactgtttc tatacctcca
      121 aaaattccag taaatatatc tatagatagt ttcaaaaaaa attgtcttag tttcataata
      181 tttaagatag tggtacatac tttataacaa cgtacttttc caaatattat tttccgttat
      241 gaacatccaa tatattacaa agtttaaaag tttagatgaa ctataatctt catttctgca
      301 cagtggataa taaattctta tagataaaaa atagcctctt ctaaagtcac aaatacctta
      361 ctcccacatt ttaagtaaac actctgcagc attctctttg ttataaaatg atagatattc
      421 ctcaatttta tctcttatta tgttattatc agttgatacc attccattaa tgtttcagaa
      481 taattacaat attttgggac cggataactc ccttttgctc gtaaccactc atatccgtta
      541 ggattcacta aaagtttacc gtttattttt cgattctttt tttgaaccgt cccatgaaca
      601 taccgattca acaagctaaa ttataaaaat tggtgtgctg tcattatttt ttggaaaatt
      661 tattaatggc tatcaatata cttttgtatc atactaaata gctcgtcagt tcctatatcg
      721 gatataccaa tattaaagca tttagatcat tatatttaag agtggtatca tcccaaaatt
      781 tcgaatattt tcttttatat aagaataatg atactgaatt gccccattca cctttctttt
      841 tttagatttt ctacaagtga ttcaaaccct ccataattac agttattact tttgaaacga
      901 taatatgaac atgtgtaatc ttttttatta acaatccatc attctatccc taactaatat
      961 gctccattgg gcttgacaat aacataaaac gtttttaaaa tcaacctcaa gtcgtacatt
     1021 aaacttcgat gaccgatata ctctaaatcc aactcaacca ttttatggaa actaacatta
     1081 tttctatcac taacttgcca caaacctgaa caaccaggtt ttacatacaa gcgttgaaga
     1141 tcatatctcg tgtactcagc tacttccctc tccaaaggtg gccgtggacc aattagactc
     1201 atatcgcctt ttaaaacatt ccatagctgt ggcaattcat caatgctttt ggcacggata
     1261 attttaccta ttttagtgac acgcggatct tctttcatct tgaacattgc accttctatt
     1321 tcattttgat caagtaattc ttccaatttt tcttcagcat ctaaacacat cgaacggaat
     1381 ttatgcattt tgaatgtttt tccattttta ccaacacgtg tatggctaaa aaatattgga
     1441 cttcctggct cttcttttct gattcgataa gctacataga acatcaaagg agaagctaaa
     1501 atcaaaccaa taatgcttcc tacaatatca atcattcttt taaaaaataa atagatatat
     1561 cgtttttcaa ctttttcttt atccaaccaa acttgattag gatgtacttc actaatacga
     1621 gataataaat cttcactttc catactttca gtccctttca ctatcttcac tctctcctca
     1681 tttgttttta tctacgaata aagaactaaa ataaaccaaa cttcttcttt tttacctctt
     1741 catagattgg ataactgatc tcatccccgt tgatcagatc ctttgcgact tgtttcatct
     1801 gtactacttt ttccttacca aagtccttga caatctgctc atagcactct tttaagtaaa
     1861 aggtgcgctt agagaccccg tgtgcatcag aagcagccat ctgcacgaga ttatgtttga
     1921 ccattgtttt ggctgttttc tggatgtctt tgccaaatac accaacataa cttggcgcag
     1981 taatttgggc caaacatccc atatctagga acggaagcaa atgatttggg tcttctcgaa
     2041 aatgcgcgtt gcgttctgga tggacgatca ctggtgtctt tcctagttct aacaactcaa
     2101 aaaaaagcca ttctgcatat aacggcactt ccagtgtcgg aaattcgatc agaagatatg
     2161 tatcatctaa atctgtaaat aaaatttcat ctcggtggat gtcttcgatg agcgttccgg
     2221 taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc tctaattcgg
     2281 cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat tttccattat
     2341 tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg aatcgctttt tcagccattg
     2401 caatacttgc ttctaagttt tctgccccgt catcgattcc tggtaggata tggcaatgta
     2461 gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttctgtag taataaccag
     2521 catctttgct atgttctgct ccgttgtata caacacctaa gactcgtgct tgtaccatct
     2581 ctagcagttc ttttgcttta gtcaaggatt ctttacgtga tgtattttca cgtacgacca
     2641 ataaagtgcc atccgttttt gaagacatga tctgtgcatc tgttacggct actactggtg
     2701 gcatgtcgaa gatcactaca tcaaatagtt gtcttgcttc tgctaaaatc tgatccattc
     2761 ttggcgagct taataactct gatggatttg gtggtttagg tccacttggc agaatcgaca
     2821 aattctcgac tggtgttcgt tggatctcat ctgctacact tcctgatgaa ctaagtgctg
     2881 tacttaatcc actggcattg ttcagctgga atgtctcata gacgacgggt ttacgtaaat
     2941 ctgcatcgac tagtagaact cgttgaccgg atttagcaaa aacgactgca atgtttgctg
     3001 caatggtgga ttttccttct ccaggacctg atgaagtcac gacaatcgtt ttgatttgct
     3061 gaccagctgc ggaggcaaac tggatattcg tccggatcgt acgatattgt tcggcgattg
     3121 gtgaagatgg gtttgttaat gtgatcaaac tgactacatg agtttgtgct gctttttgtt
     3181 tctgtgttcg tgccattttt cttcctccta gactcttgag cggcttctgc gtgatggagc
     3241 tgttttatcg tttgtagctt ttggtcgtgc aggagctgat ccttttttac ggataccagc
     3301 gttcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc ctaatgtatc
     3361 cgtcacatat ttttcatctt tcactgtccg atcaagtaat tcgaataaga atgccaaacc
     3421 tacgcctacc atcattccta atactaagcc aatcactaga ttcaatttat tatttggcga
     3481 aacaggtgtc gtattggcaa ctgcacctga gatgatcgag attttatcga cattcatcac
     3541 gtcttttgcg ttctcttgga aaacttgtga agttgtatta gcgatctgtt gagcagtcac
     3601 tgcattcgtg cttattgcct ggatcgagaa catttgtgag ttttctgact gattgacgga
     3661 gatcgcatct ttgatttgtc cagcggtcat tttcacattg tcttctgttt ctagacgatc
     3721 tttgacttca tttaatacta gatcactaac gatcatatct ttatacgtat tgatcatttg
     3781 caggttcgta ttgacatcat tgacattcgc tgtgtctgat tgcggcagtg tcacgatcaa
     3841 ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc ctgagatggc
     3901 caagccagca aacattgaga tgatgattgt tgccaagtgt ttgcgtaatg ttccaaaaat
     3961 ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat tctctagatt
     4021 catttgatct tttaattctt ttttcacacg atcaagttct tgatcgctga ctttttgata
     4081 ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttga tcgtaccaaa
     4141 tgcctcccga taatctaaag cgattttttt catttcatca aagctcatgt ctgttttcat
     4201 attcgattct agagcattca aaacggattg atagttgctg acaccgttta gactcaatac
     4261 ttttttcgct acaccttcta cgatctttct ttgacgctct tgtctaccat agtcaccatt
     4321 tggatcttca taacgcatac gagaataaga taatgcctga tccccgttca atgtgatttt
     4381 accgattgta aagtcatagc catcttgtga gaaagtgagg tcattattga cttcgatacc
     4441 accaactgca tcaacaagtt cttttagccc tttcatattg atagagacat agtgatcgat
     4501 tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc cgaatgcgta
     4561 agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg tatctcgagg
     4621 aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca tcatcgtatc
     4681 tgatcttcct tgatcgaccc gacctaaatc tccagtatca atccccatca atagaacaga
     4741 gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg ttcgttccac
     4801 tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg ctcctactcc
     4861 tacaattgct aatactaata cgataagaac tgctaaaata atcagaaata tttttttccc
     4921 tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt gcttgaatca
     4981 tcgctttaac tgtcaatagt ttatattgct atagcccatt tgctgagcaa cagtttgtgc
     5041 ttttgttgcg tatgctacgc ttattttttc attccggatc tgctctattt gactgagcgc
     5101 cgtcaaatat tgatcgtaag tagcggtggc agtcacttga ccatccttta acatcgtatc
     5161 gaataactga tctagttgcg tgacttgcgc gatctgggta tcagcgaatc ctagatattg
     5221 cataacaacc gttttccaag gactatcttt aaaaaagctc atattttcac gtacacgagc
     5281 gacatctgca cttgtcaccc tttctttgat gatgacatcg tctacagctt gctgccagtt
     5341 tgatacattt ttagtaaata aatcgttgac agctgtctgg atcttcagtt ttgattctgc
     5401 actatctaac tgtgtcacga ttgctttctt ctcatcttgg attgcaacag cttctttagg
     5461 caattctgat tctttgatgt cgtaatcatc tgctgaaagt ttgacagagg atagcttatt
     5521 tgacaaactt gtgattgttt cttctgttat ttcagggttt agaaaaattt tttcttttgt
     5581 actatacagc ttgtctactt cttttgataa agaagtcaag gtgtcttttt gattggtgat
     5641 ggcagattct gcataattga tccgttgctg atgttgccgc tgattattga tatggtatcc
     5701 aaacaaacag atcgcaatag cacctgcacc taccgccaca gcagtcaatg acttgacgaa
     5761 tggcttttcc atagattcgc tcctaagtat tgatgataga taaattaaga tataattcac
     5821 ttacataaaa atgacagttt tttatttaaa aaaacataat taaactaata tattcacaga
     5881 aatatttgta aacaagtttt ccctatataa tcataccaca aactgctttc ttgactaggg
     5941 aaatttacga tttaaaagta aataaaacat taataagata acaattaatc gattttactt
     6001 ttatttcctt aattttcata ggtgttttta aaagttacac aaaaaataaa ataacaaaaa
     6061 aaattaacat tgaattaata atagttattt ataacaaata aaccagagaa atagcctatt
     6121 ttgtgcctgt tctctggttt atttacatat tagaaattat tttgtgatga tttgatctaa
     6181 acttgctagt gacaaagcca tggcagcaat ttgacttaat tgcgttaatc ggttcttacg
     6241 tactgctggt tcttctgcca tgaccatcgt ctgatcaaaa taattttcga tcagtgggcg
     6301 aaggcttaca agctgatcat aattttcgtt gatcgtttgg tcagcaaagc ttgttttcat
     6361 tgcctctact gctttgtgca gttctttctc cgcttcgttt tcgaacagtt cagggtctac
     6421 ttctgattgt ttttcaagct ccgctttttt cgctaagttc acgacacgtg tcaaagcttc
     6481 catcgatggt ttgaagtctt catctgcaag atgctgcttg aacaattgtg ctgctgcaaa
     6541 tacttttgtc agatcttttt gttctgcatt caaaacagct tcgatgacat catgacgaat
     6601 attcttcgtt gtcaataatt ggcgcagacg gcctttgata aagtcgatca cctgttgttg
     6661 tcccgctgac aagccaatgc caaaacgatc cgcatcttta ctgataactt cactaatcgt
     6721 ttcttgcaga acagataaag gaaaggccca ttttttcgat tcgatgattc gaacgacacc
     6781 atatgcttga cggcgcaaag catatggatc gtttgagcca cttgggatca tcccaactgc
     6841 aaagaaagaa aagatactat ctaatttatc agcaatcgct aaaacagaac ctactgtagt
     6901 ctcaggtaga tctccttcac tggatatagg catgtaatgt tcacggattg ctgttgctac
     6961 ggctggtttc tcgcctttaa gcagagcata tttttctccc atgatccctt gtagttcagg
     7021 gaattctcct accatgttgg ttactagatc aaatttatag atttcagcag cgcgttgcag
     7081 atccatcaat tcttcctcag atagtcctac ttgctcgcct atgatgcggg cgatgacttg
     7141 tacccgttgc attttctcgt agatgctacc gattttttca tggtaagtaa cagtttttaa
     7201 tttttcaacg tattggtcga ttgaagcttt cttgtcttca ttataaaaga attccgcatc
     7261 ttctaaacga gcagtcaata ctttttgatt gcctcgaacc acattttcca attttacccg
     7321 atcaccatta cgagcagaaa taaaatgagg aagcagcata ccttgatcat tccgtacctc
     7381 gaaataacgt tgatgttctt tcatagaagt gactagtact tcttcaggaa cagaaagata
     7441 tttttcttcg tagtcgccag aaaagacagt tggatattcg actaaattgt tgacttcttc
     7501 tagtaaatct tcgtctagat caataaccca gtgatttttt tctgctaatt cgtttgcttg
     7561 tgtaacgatc atttcttgtc ttttttgcgg agacgcaatg acgaattgtt cattcaattt
     7621 ttcttcatat tcttttgcat gcgcaaatgt cacatcttcg cctaagaaac gatgacctcg
     7681 agaggctctg cctgttttga catccaagat ttcgaatggg attacttcat catctaacaa
     7741 tgccacgatc cagtgaatag ggcggatata ttcaaaatcg tattctgccc aatgcatcgt
     7801 tacagggaaa gtcaggcttt taatgacatc tgctaaacct gtcaatactt ctttggattc
     7861 cttaccatga gtgtatttcg ttacatagac atattccaca ccttttaatt ctctaaaagt
     7921 gatagcgtct gtcgtcagac cttgtccgcg gacaaatcct tccgctgctt tcgtccagtt
     7981 tccatctgcg tccaaagcaa tttttttggc tggtcctttg acttcttctt cactgtccgc
     8041 ttgtcgttct ggaatatttg ttactttgat tgctaaacgg cgcggtgtgg aaaatgtttc
     8101 gatcgtatca taagtcaatt ggttttcatc taaaaatttt gctgtttttt cttctaattg
     8161 tttcatgctt ggtgtgacaa catgggcagg catttcttcc agcccgattt caagtaataa
     8221 gtcttttgcc atcttattca gcctccttca cggtttctgg atttgctgtg tttttattca
     8281 acaaagggaa tcctaatttt tcgcgttctg ccacaaagat tttggctaca gcacgagcca
     8341 tgttgcggat acgggaaaga taaccggcac gctctgttac ggatactgca ccgcgagcat
     8401 ctaataaatt gaatgtatgg ctgcatttca aaatataatc atacgctgga tgaacaagat
     8461 tttcatcgat acagcgttta gcttcttttt caaaacgatc aaagttgctt agtagcaagt
     8521 cttgatcgct tacttcaaaa gaatatttgg aatgttcata ttctggttgt ttgaagatct
     8581 cgccatattt cacgccatca gcccattcta aatcatagac actttcaact tcttggatat
     8641 aagaagcaag acgttctagt ccataagtga tctcagaagt gactggttta caaggcagtc
     8701 caccaacttg ctggaagtac gtgaattgtg tgatttccat tccgtcaagc catacttccc
     8761 aacccacacc ggcacagccc atggatgggt tctcccagtt gtcttcaaca aaacggatgt
     8821 cgtgttctaa tggatcgatt cctaaaaggc gcaagctttc taaataaagc tcttggatat
     8881 tctcaggtga aggtttcatc acgacttgga attgatgatg ctgatacaaa cggtttgggt
     8941 tttcaccata acgtccatca gctggtcggc gagatggttc tacgtaagca gcattccatg
     9001 gttctggtcc gattgcccgt aaaaaggtat atgggctcat cgtacctgct cctttttctg
     9061 tgtcatacgc ttgcatcagc atgcagccgt ttgatgacca aaatttttgt aatgttaaaa
     9121 tcatatcttg gacggtcaat tttttactca tctgttttgc ccctttcaat tttaaagcgt
     9181 tgctgtcaaa taagaataca ggaaagaaaa cgacaaaaaa tccctatgtc tgattacaga
     9241 catagggacg aataattcgc ggttccaccc tacttccgaa taaagattcg gcagcttcat
     9301 ttatcgtact cgcaagtgcc tcttcaaaaa tcgtttgggt ccggctccca ctctcccgaa
     9361 ctcgctaaac cacgtgtttt ttactgatct tggtcaatgt acgtattcat tttgcagttt
     9421 tcttgtttct ataatagatc ctctcactga acgatctatt tgatctacgg ttaataatag
     9481 acggaactcc ctgtgtttgt caaatggttt tgattttttg ttgtatgttt catttactat
     9541 tcttcttttt taggtagatc tttttcagac atgtctttct ctggcgtctt taatacgtgt
     9601 gcccaagact tcatttgatc gatgaacttt ttgcttttca aatgaatacc gacatattca
     9661 tcatacagct ggtcgatgag ttcacggatt gcttgtttgg tttcttcttt cagatggatt
     9721 ccactgattt tatcataaga aatagctgaa aacatccgaa cgaaataaac agctcgagga
     9781 tctgcatgat aacggtgatc gtccatcgac caatgtcttt cgcatatcac accaccatat
     9841 ttggaagaat agtcaaattt tccttttgtt tccccacata cgacgcagtg tgtccagatt
     9901 ggtgcaattc cgaagcgttg cagtatttga acttcaaaga tattcgtgat gatttctgca
     9961 tcatctcctt gatccatccg ctgcagagct tgttgcgtaa attggaataa atgcgggtca
    10021 tattgatgat cttcgattgc tgcatctact agatttagca aataggtacc ataaccgctc
    10081 aaaaagatat cttcttggat gttccgaaac gcatggacgt ctttgacact gcttaaaaac
    10141 gacaaacctt cacttcgaaa attaccgata tagactgctt cggtaaaggg gagcaaagcc
    10201 ggagctaatg gctgattttg ccgatggatt cctttgatat aaaacatcat tttgccagaa
    10261 gattccgtaa agattttgac taatttatct ttttctttgt aattacggct agataaaatc
    10321 aagcctttcg attctgcaag ctggctcata ttctccctcc taaaaaataa atctggaaaa
    10381 gcagattctg ccgatccaga ttatttatgt ttcgatcttc cgccgttttt ctcaacgtgc
    10441 ggatttttat ccagtttatt tctatactta acctataaga agttcagaat atcagtattc
    10501 atcttttcgg tagccgaagt cttgcagata gacttttttg tctcgccagt ctttttggac
    10561 tttgacccaa agttctagaa atactttgtc tcctaaaaga ttttcgatat cctttcttgc
    10621 tttcgttcct acttgcttca gcatttttcc gcctttgccg ataatgattc ctttttggct
    10681 atctctttcg acgatgatcg tggcctggat atggatttta tcattctcgt ttcgcttcat
    10741 cgaatcaaca acgactgcga cagaatgagg cacttcgtct cgtgttaaaa acaagacttt
    10801 ctcacggatc aattcagaaa cgatgaagta ttcaggatga tcagtcactt gatcatctgg
    10861 aaaatattgt gggccctctg gcatttcact gactaataca tccatcaaac gttcgacgtt
    10921 gtttccttct gtagcagaaa ttggaatcac ttgtgcaaag ggcatctgcg ttgaataatc
    10981 ttcgatgatt cccaacaact cgtctggatg gattttatcg attttattga taatcagata
    11041 aacaggagaa tgcacgtttt ttagacgttc aataataaaa tcgtctcccc ggccgcgttt
    11101 ttgatcagcg ctgatcataa ataagaccgc gtccacttct ttcaacgcac tgtaagctgc
    11161 ttctaccata aaatctccta aacgatgctt tggtttatgg attcctggtg tgtcaataaa
    11221 aatcaactga gcttctggtg tagtatatac tccttggatt ttatttctag ttgtctgcgc
    11281 cttatcactc atgatggcaa ttttttgacc cacgattcga ttcaataatg tggattttcc
    11341 tacattcggg cggccgacga ttgcgacaaa ccctgattta tgttctggca tatacgttct
    11401 cctttttaat taaacaacaa atgatatatt ttagggacaa ataaaatcaa cccaacgatg
    11461 acggcaaaac aagcggtcac taaaactgct ccagctgcca tatctttgat ttttttccca
    11521 atcggatgaa aatgaaaatc cgtaaacatg tctacgacat tttcaaaaac agtattgatg
    11581 atctctacta tccatacgag aaaaacagct aaaagcagcc ataaccattc tatccgatca
    11641 agttggaaaa tcacgcccat aaacaaagcg aggattccga acgcaacatg tttcctcatg
    11701 ttgcgttctt cctcaaaaac agttttgact ccttggacgg caaattccac ggacatcagg
    11761 aaatgtttgt ttttctctac gtttttatct tttaagtcca taggcatcta agatctcttt
    11821 ctgtaaacca aacatttctt tctcatcctc tggtgtcata tggtcatacc cgttgatatg
    11881 caagaatcca tgaaccgcta aaaagccaag ttcccgatca tacgaatggc cgtaagcttc
    11941 tgcttgctct gcagcacgtt cagtagagat catgatgtct cccagtacac gaggcaatgg
    12001 attctctcca tcttcaaaaa tcactgggat ttcgtcttca ccctcttctt ccaaggcaaa
    12061 actgatgacg tctgtcggtg catcttttcc acggtaatca cgattgatta cttggatcgc
    12121 tgcgttgtcc atgaaagtaa cagacatttc tgtttcttct ggcaactcta aatatgatgc
    12181 tgcaaattgc aacaagtcgt cgatttcttt cattttctct tctgagactt ggtttgtttc
    12241 atccatgaag gtaatatcca ttatacatcg cttctttcta ttcttcttgc tattcttctt
    12301 gttcttcttt tatcttctca acttccgatt tcattttagg atatttgatc cgagaatgga
    12361 aggtactgct taaaccgctg atcaatgatt tttctacttt acggatttct tttaacgtca
    12421 atccactgtc atccaattgt ccatcggaaa tacgctcttc tataagatta tggacaaaac
    12481 tttcgatttt ttcgcttgtt ggatggtcca ttgcccgtac tgccgcttca caactgtcag
    12541 ctatattgac gattcctgct tcacggcttt gaggttttgg tccaggataa cggaattgct
    12601 cttccgtgac ttccgggttg cgttcctttg ccttaacgta gaaataacgc atcagcgtgg
    12661 ttccatgatg ctgtctgcaa atatcgatca ccatttgcgg catattataa tcttccaata
    12721 ttttggcacc atctgtcaca tgtccaaaga taatatgttt actgtcttct ggcaataaga
    12781 aattgtgcgg attttccgct cctgaaggca agttctcaac gaaaaagctg gcgtgtttga
    12841 tcttgccgat atcatgataa tagcaagcta ctcgagttag taaagaacgt ccaccgattt
    12901 ctgctacagc attagcactt agattggcta ccatcatgct atgatgatac gtacctggtg
    12961 cttcttctaa caactgtttc aataaagggt gattaggatt gctgagttca tttaatacga
    13021 tcacactatc atcattgacc atcaattcaa tataggggtg cagacctatc gtcagcaaga
    13081 atgagaacaa tgcacctgtg aatccgcaga ctagcgttaa ccaagtcgtt gcatccccaa
    13141 agctcattcc ttggtagata atcaaaatga catccatcat cacagggaaa ataatgaccc
    13201 acatagcagc gaataaccct tgttctgaga tccgtttgcg tctgacgatc gttgccatga
    13261 gaccagaaaa tacataggac atcaaaataa ctgtcagaaa gttcgtgcca atcgaatcat
    13321 aaaaaatgaa aagtgcggat actgcttgga aaataccagc catgatccct gctcttcgat
    13381 tcaagaaaaa gcttaaaaca agtggagcaa aagccgcggg gtaaaacagc ggaatataag
    13441 cagcttgttc tgtttggaag agttggaaaa atttcattag tactacgctg actgacattg
    13501 ctgtcacgta gaaaagtacg tattttgttc tttctcctgc attttgaaat tgaagtgaat
    13561 tataaatcag tacaccgatt tgaagcaaaa cagctaaaac catggcaatg agtgggaaaa
    13621 tcgaagtcgt ttgattcgtc aatcctagta attccaattt tttcatcgct gaagcatcaa
    13681 tttgatttcc ttcgcgaaca atgatttctc cttggaagat catcactggt tgtacagctt
    13741 ctcgtgcaga ttgttttaat tcttctgtct ttttctcatt caatacatca ttgactacaa
    13801 tgccttgatc cactaagtag cggatcattt gttgctgctc tgttgtgaca ttcaaatact
    13861 gaatctgatc ttctgcttct tgtttaaaag cttccaattc gctttcacga acttgtttac
    13921 tcatctgttc gtcaatgagc ttcaaacttt catccctaac cgtgtgaatt tccgtttcag
    13981 tcatttcaaa tatcgtcgaa taaaaattgt tcggcagttt ttggtaaaac gcaacgtttt
    14041 ctgcattcac gttttcaaaa tttttcttca gtgtagcaat ccgctcttct gtagtaggtt
    14101 tcgggatatt ttcatcttcc ttcgccttat ctaccttgtc attataagat ttgttgatgg
    14161 tatcattcgt cttatcgatc aattcgaata attgcttgat ccgattgttc tggtcatctg
    14221 ccagatcttt ttgataagta tattctggag tcactgcttc tgctgctagt tttcttcttt
    14281 gttcggtttc ttccgtgttc tcgatcgttt tattcgcacg gatactctct tctgctactt
    14341 ggccttctct aaaatcattg gccttttgtt tgacactact aaatgtaacg ccgcaaacaa
    14401 tgataaaaaa caagaacaga aggaaaggca atgctgcctt tccgagtttt tcttgtagtt
    14461 tctgcagtgt tttattcaac atgtgcttat cctcctaagc aaagcttatt cttgatgaag
    14521 atccgccttt tcataagctt tgatgatttc ggcaactaca gggtgccgaa caacgtcact
    14581 tgcttcaaaa ttaacaaaat cgattttttt gatttgcttt agtgttcgtt ctgcatgaac
    14641 aagaccactt gtcgtccctc gaggcaagtc tatctgactt gtatccccat tgacaatcat
    14701 ttttgattga taaccgagac gggtcaagaa cattttcatc tgagcgactg tcgtgttttg
    14761 tgcttcgtcc aagataacga atgcatcatc caatgtccgc ccccgcatgt aagctaatgg
    14821 tgcgatttcg attacgcctc gttccatcaa tcggttcgta tgatccaatc caaaaatttg
    14881 gtataaagca tcataaacag gccgcagata aggatccact ttttctttca gatcccctgg
    14941 tagaaagcct aggttttccc ctgcctctac agcgggccgc gtcaaaatga ttttttgcac
    15001 ttcgcctttt tttaaggcag caattgctaa tactacggca agaaacgttt tccctgttcc
    15061 ggctggccct acaccaaata cgatgtcatg tttggctacc gattcaacat atttcttttg
    15121 accgctgttt tttacccgta taggttttcc attacggtct ttgacgatct cttcctcata
    15181 catctcaaca aaataatcta atgtaccttt ttgccccatt ttcaatgccg tcacaacatc
    15241 gggcgtactt acgtggattc ctcgtttgat caattcctgt aatgtccgaa tgactgaggc
    15301 agccaaggaa atctgggatt gttcaccaat gatctgaatg atttctccac gtgtatggat
    15361 ggttgtttcc gttgtttcct cgatcagttt tatatgcttg tcgtgtgcac ctaaaagcat
    15421 gctgacatca tcagcagctg tcaatctaat ctctaaagaa ctagactctt ccgtcaaaaa
    15481 tagacatccc ctttttttcg attcattctt tcctatttta ccatactcag aagaaactat
    15541 atagcaaata agaaaaggtt gtaaaagagt cgtttaacca tacgtaccaa gaaacgttaa
    15601 aaaatagccg tttccattcg ctaaaggtct cgttttgata aaaagaatct gagaatgtaa
    15661 gttatatgaa agtggagaac aaaatattcc gtttcattca gcaggtattc tctaggtatt
    15721 tttcttgttt caaaaaaaga aaacaaaata gttaaaatat tcttgcattt caaaaaaaac
    15781 aggtgtatta ttacgtcaac aaataaacat acagcaaagc gtcgaggaaa acagtaggct
    15841 attcaacgat ttattcccag tgagtcatgt aagtgagaaa tgacaataaa tcgaatagac
    15901 gaacatcatt tccgagtcaa tgtcgaaaac gtagatcatt gtggctgcct ccattatcag
    15961 gctagcggat aactttatca aaaagcgtcc gtgaatgaag aagctttttt aagcgaaaaa
    16021 aggtggtacc gttctagaat ctagagccct tttcccaagt ggaaaagggc tttttttata
    16081 ttttaaggtg gtggttttca tggaatctga tattaatggc attactaatt gctgcaacgt
    16141 ttatatcgtc aatcaaaatg ataattattg gaggaaaaat ttatggaaga aacaaaaact
    16201 tattcatgga aacaactatt gatggtcagt tcacttatct tcgggatgtt cttcgggtca
    16261 ggtaatctaa tctttcctgt ccacttaggt caaatggctg gcacaaactg ggtcagtgct
    16321 ggcgtaggat ttgccatctc aggcgctctt ttcccattac tagctatctt agctgtagta
    16381 gtgacaaaaa gtgatggtct gtatgactta gctaaacctg tcggaaaatg gtatgctgcc
    16441 ttgttcttaa tccttgttca tttgacgatc ggaccatttt tcggtacacc acggacagct
    16501 gctactgctt ttgaaatggc tgctaaacca tttttgcctg aaaaatataa ttcattaggt
    16561 atgtttttat tctctgctct tttcttttta cttgcttatt tccttactgt ccatcccaca
    16621 cggttggtca aatacgtcgg aaagtatcta aactccttgt ttcttgcatt attagcaatc
    16681 atttttgtcg tttctttatt ccacccaatg ggaaatctaa atcaatctgc tgcagatact
    16741 taccaaacaa acgctttaac aaatggtgtc ctagaaggtt ataacacggt tgatgcagta
    16801 gctttgcttg ccttaagtgt cacttttgtc catgcagtcc gtgaccttgg tttcaaagat
    16861 aaaaaagtca cagaattaac agcaaaagct ggtagcctga gcgttgcgct agaagtcatg
    16921 atctattttg gacttgttct tctcggtgcg atgagtttga ataagatgtc tttatcagaa
    16981 aatggcggaa ctgcactttc tcagattact aatttttatt taggtaactt cggcgctagc
    17041 ttcttaggag tgatggtcac attaggtgtc ttcactaccg ctatgggact cgttgtttct
    17101 tttgcacaag atttccataa actgttccct aaagtcagtt atatgacatg gctgcggtta
    17161 acgactttcg tctcttttgt cgttgcaaat gctggtttgg acaatatcat ccaatggtct
    17221 ttaccagtat tgatgctttt gtacccattg tcgcttgcgt tgatcttgct ttccttaaca
    17281 gcaaaatttt tccaaaagac accatttgtc tatcaagtaa cgatgttgtt tgctgctgtt
    17341 cccgccgtat tggatatgct ggcaaattcg cctgctttag tgagtcaaca aagagttgta
    17401 gcaagcatgc tcgaatttta tcatcatcac gttccttttg ctgcattagg acttggctgg
    17461 atggtcccaa cgttacttgg ttacgctggc agcttactct tctattacgc ctatcgccta
    17521 tcagggtaca aacaagaagc aaatgaacta ccagaagaat aaccagatca aacacaattt
    17581 aacataagaa atattaaaca ggaaaaaacc agctagatga aagaatccta ttctttcgtc
    17641 tagctatttt ttagatttct tttttacgag cacaaaaaaa ctgcggacaa tgattgtccg
    17701 cagtttaaat gataaaatta agattgtaat agttctttca caatggcatt tacttggttt
    17761 ccatctgctt ttcctttgac tttaggcatg atcgcaccca tgactttgcc aaattctttc
    17821 attgatgaag caccagtttg tgtgattgct tcttgaacaa gttgtcgaat ttcttcgtcg
    17881 gaaagttgtt ccggcatgta tttttcgact atcacaattt cactttttac tttttcagct
    17941 aaatcgtcac gtcctgcttc ttcaaactca tgcaaagaat cacgtcgctg tttcatttcg
    18001 cgagacaaca ccgttaattc ttcttcttct gtcaagtcgt gaccttcttt aatctgctcg
    18061 ttttgtatag atgacttgat cattcggatg acttgtaaag attccttatc tttcgctttc
    18121 atcgctgttt tcatgtcatc gtttaatcta cttagtagtg acacactacc aactccaatt
    18181 catcacagaa ctagaatttt ttgcgttttc tagctgcttc agattttttc ttgcgtctta
    18241 cacttggttt ttcgtagaat tcacgtttac gagattcttg taaagtaccc gcttttgaca
    18301 cggaacgttt gaagcgacga agagcatcgt caagtgattc gtttttgcga acgactgttt
    18361 ttgacatgtt aattccctcc ctccgagctt aaaaagtaac cgtaattgtt attgaaatat
    18421 atctcaaaaa acaagactgt cctgtaaaca ttatatctaa tgctgttttg accgtcaagg
    18481 aaatcttgct tttttttaaa attatcaaat gttattttat ttaaatcctt attgacaatt
    18541 ttcacatctt ccgaaaatct cgaatctatg cccttcgatc gtacatccac cgagctgttc
    18601 ttcgaaataa ttcattggac acatatggat ttctttcgtt gttccgcaca ccgtacagat
    18661 aaagtgatgg tgatggccga tttccccttg acagcaatga aaacgaaact tcatctctcc
    18721 atttaaatcg gtttcttcca gtaaatcgat ttctgcaaaa tcatggagat ttcgataaat
    18781 cgtatcgtaa cttactcctt tgaattcctg attcatatag tcaaaaactt ctttagcgga
    18841 tacgtagcga ttgcgcttca ctaaataagt taacaatgct tctctttttt tagtatattt
    18901 aaaaccgtta tttttcaatg tttctagtga ttgttcgatc aatgtctgtt ttttcatgac
    18961 ttattcctcc aaatcaaaat gattacgaag tacacattct agagtatggt ataataaaca
    19021 taaaaaaaat gcaagtgagg ttttatccat gtcttccgat tctacagaaa tcgttacttt
    19081 ttttgtcatc tccgactctg ctggagaaac tgcgacaaaa ttagcgcaag ccactatggc
    19141 ccaatatcct agcgttgagt tcaatttatt ccgcagaaca tttgtgacag atgagaaaac
    19201 attgatgcag gcactgcaag atgcactaga agaaaaagct cttgtcttac acacattgat
    19261 caatcaagaa ttgatcgaga tgacgcgttc tttttgtgaa gaacatcaat tatttagttt
    19321 tgatgtgatg actccgccga tatcagaaat cgagcgctta actggtatca agcccattcg
    19381 gcaacctggc gcccttcatt tattgaatga gaactatttc aaacggatta aagcaatgga
    19441 atttgctgta aaatacgatg acgggaaaga tcctcgtggg tttctagaag cagatgtcgt
    19501 tttgctaggc gtatcccgaa cttccaaaac gcctttgagc ttgtttttag ctaacaaaaa
    19561 tttaaaagtt gccaatcttc ctttgatccc acaagcacat attcccaaac agctttggga
    19621 gattgatcct aaaaaaatcg tgggattgac gaataaccca gatattttaa acaatatccg
    19681 taaagaacgg atgcgctctt acggattaaa tcctgatact gcttattctg atatcgaaaa
    19741 aatacgagct gaactcgatt ttgcaaatga tctttatgaa aaactgggct gcgtagtaat
    19801 caatgtcgct tctttatcta ttgaagaaac agcttcgctt attttgaatg ctttagacct
    19861 agaagatcat agttattatg gaactgaaac caccgaagaa acagactaac agtttttctt
    19921 gtctttttct taaaaaaaac tggactgtgt cagcgaagaa gattcgttga cacagtccag
    19981 tttgtctcta tttgcaccta tcgttattga tgttccacat gtttgagtgt ctggttcaaa
    20041 atatccagta cgtgagaatc atcaagacta tacaaaatcg cttttccctc acgatgggac
    20101 ttgacgacat ggttttctct taataaacgc agttgatgcg atacagcaga ttgttccatt
    20161 tccactgctt gggtgattgc tgtcacattt tgttccccgt ctttcagata cagcagaatt
    20221 ttgagacgag tggggtcact caacattttg aatagttggc tgactttctc tacttttgtt
    20281 ttttgcgtgt ctggcaatcc attcacctcc aacttgaaaa aagactgact tctaaagttt
    20341 accacttttt ccatcagtct caagtttttt tattcttctg tcatcttttg gataatcgct
    20401 tcataagcgg ctactttttc agctgcgtct tctgaagagt ttccttttac accgatgtaa
    20461 aatttgattt tcggctcagt tcctgaagga cggatagcga tccagccatt ttcttccagc
    20521 gtatacttca atacatcaga tggcggtgtc gttagttttt ctacgtttcc ttcacggtct
    20581 gttctcgtca actctttgaa gtcttctgtt tggatgactt ttgctccggc aaattctgtt
    20641 ggtgcttctt cacggaattt ttccatcaaa gcagagattt tcgcagaacc ttcttgtccg
    20701 ctcattgtca cagaaatcgt tttttctgcg tagtagccat aggtttcaaa aatcgtttgc
    20761 aagccgtcat acaacgtttt gccttgtttt ttatagtaag ctgctacttc tgctaataag
    20821 agcaatgctt ggatcgcgtc tttgtcgcga acgaaaggtt taactaaata accgtaactt
    20881 tcttcgaatc cgaacataaa agtatgggaa tgatcttctt caaattgttg aattttttca
    20941 gcaatgaatt tgaatcctgt caatacattg aacatggtag ccccatagtg ttccgcaatt
    21001 gctgtaggta attcgctaga aacgatcgat ttcaataccg ctgcgttttc aggtaaagtg
    21061 cctcgcgatt ggtgtgcttc caaaatatat tgagcaaaca gtgctccgat ttggtttccg
    21121 gtcaatactt cataggagcc atttggcaaa cgaacggctg cacctaaacg gtctgcatca
    21181 ggatctgttg caatcagcaa atcagcgcct tctttctcgc caagtcgtat cgcatactca
    21241 aaagcagagt gttcttctgg atttggtgat ttgactgtag tgaaatctgg gtctgcgaca
    21301 gcttgttctg gaactagtac gaattgttcg aatccagctt gtttcaacgc acgttcgccc
    21361 aacatttttc ctgttccgtg aagtggtgtg tacacaagtt tcaaatcttt ccccattgtt
    21421 tccaccaatt catgatcaat cgttactgct ttgatttctt ctagataagc ggcatccact
    21481 tcttctccga tgatcgtgat caatccgcta tgttcagctt cttcgtcaga taatacttca
    21541 acttttaacg gattgctgac ttcacggaca tatttcgtca atgcatctgc gtctgcagga
    21601 ggcatttgtc ccccatcttc gccgtagact ttatatccgt tgtaagctgc tggattgtgg
    21661 gatgcagtta ccatgattcc cgcaaacgtc ttcaaatgac ggactgcaaa agaaagctct
    21721 ggtgttggac gtagactttc gaatacataa gatgggatat tgtgatgtgc taatgttttg
    21781 gctgcttcca tggcaaattc tggtgattga tggcgtgagt cataagcgat agcgacacct
    21841 cggcgttttg tttcttcgcc ttgtgtatcc atgaaacgtg caagtccttc tgttgcttgt
    21901 cttaccgtat agatgttcat tcgattgatc ccaggcccta agattccccg catgcctgca
    21961 gtaccgaatt ccaacggtgc ataaaaagca tcttcaagtt tttcaggatc ttcacccaga
    22021 tcatttaatt gtttttttaa gttttcttct aaattttctt catgtaccca ttgttcatac
    22081 acttgttccc aagacatgaa aataacacct ctttctgaaa tatggttctt aataagtata
    22141 gcattcactt attagatata aaagtatgag gaccaaaaaa cgcttaaatc aagaaaggat
    22201 tcttttatca agcacggttg caagtattcg cataaatcgg attcagtaga tttcacgcga
    22261 aaaaaaagag taaaagaaca attgaaaccg ttcttccact ctttttaaac ttatgcagtt
    22321 gtttcttcta aagattccaa gtatttatac acttgttgtc cagcgatccc accatcacca
    22381 acagcagtag tgatttgtcg caaatctttt tcacgaacat ctccgatggc aaaaacgcct
    22441 ggtatattcg tcttcatttc gttgtctgtt ggaatccagc cagcttcgtt cgtcaaccct
    22501 gcttctctaa atggtgcagt taatggatcc agtccaacat agatgaatac tccgtctgct
    22561 gccaaatcaa actcattgcc ggttttgaca ttacgtgctt tcacacctgt cactaccatt
    22621 tcattcccaa cgatttcgtc aacgactgta tcccacataa aggaaatttt ttcattcgca
    22681 aatgcacggt cttggatgat cttttgtgca cgtaattcat ctctacggtg gacgatgact
    22741 acttcagaag caaattgtgt caaatagatg gcttcttcta ctgctgagtc tcctcctccg
    22801 atcactacta gacgtttgtt acggaaaaac gcaccatcac aaactgcaca gtaagaaacg
    22861 ccacgtccag caaattcttc ctcgccaggt acgcctaatt tacggtggat acagcctgtt
    22921 gcgataatga ctgtttttcc ttcgtaagtt ttatcttcac aaataacttc tttgtggctg
    22981 ccgtgatcct tgatatcttg cacgattcca taagcatttt ctgtcccaaa tcttgaaaca
    23041 ccttcataca tcttataagc tagatcagga cccatgattg aatcgaaacc aggatagttt
    23101 tcgatttctg ccgtattgtt catttggccg ccaggggcgc cttgttcgat catcaatacg
    23161 gataagtttg aacgtgaagc atataaagca gcagtcatcc cagcaggacc tgcgccaata
    23221 acgattacat catacatatg tagattctcc tttacttaat caagtaagca tactttacag
    23281 ccaatgcgaa aaaaagtcta ttgttctgct tgcgtttctc gaatagcttt attatactcg
    23341 aaaaatccgc tgaaaaacaa tagactttta taaatcattg atcaatcatt cgaaagattt
    23401 tctccatttg aatcaattac ttttttatac cagttgaatg atttcttctt cgaacgggct
    23461 aatgtgccat ttccctcgtt gtcacgatcg acataaatga atccgtacct tttcttcatc
    23521 tcacctgtac cagctgatac taaatcaata catccccaag tcgtatagcc taatagatcg
    23581 actccatcta attctactgc atctttcatt gcttggatat gtgctgccag atagtcgatt
    23641 cgataatcat cttccacata accattctca tcgggcgtat cgactgcacc taagccgttt
    23701 tctacaataa atagtggttt ttgataacga tcataaatat cattcatcgt tattctcaag
    23761 cctaatggat caatttgcca accccattca cttgcttcca gatatggatt tttgacagaa
    23821 gcaaagatat ttcctgcagt ctgttccaag agttcgttgt cagtagtcga aactcgagaa
    23881 gaataataag aaaaggagat aaaatcaaca gtatgttctt tcagcaatgc ggcatcccca
    23941 ggttccattt cgatctgaat gccatttcgt tccatttctt tcaatgcata agcgggatat
    24001 tctcctcgtg actggacatc aatgaagaaa taattttctc gatctgattt tctagaagcc
    24061 caaacatctt caggattgca agtgtatgga taatatgctc ctgctgccag catacagcct
    24121 actttgttct ccggatctac ttcatgagca atcttcgttg cgatggcact tgcaaccaac
    24181 tcatgatgtg ccgcttgata tttgatttgt tcttcatttt ctccttcttc aaaatacagc
    24241 cctgcgccca tgaatggtgc atgaaggatc atattgatct cattgaatgt caaccaatat
    24301 ttgacaagtc ctttatagcg attgaagatg actcggcaca gattttcata aaaaccaacg
    24361 agttctctgg aacgccacgc accatattct ttgatcaaat gcatcggaca atcaaaatgt
    24421 gtaatcgtca caagcggctc gatcccgtac ttttgacatt ctttaaagac atcctcataa
    24481 aatttcaacc cagattcatt tggctctttt tcatcgccca ttggaaaaat acgactccaa
    24541 gcaatggaca tccgataagt cttgaacccc atttctgcaa ataaagcaat atcttcctta
    24601 taatgatgat acatatcgat cgcaattttt gctggataaa aatgattttc atcaaattca
    24661 aacattttct tttttccagt aatgacagga aaacggtctt ctccgattgg cacaacgtct
    24721 acgttcgcta acccacgtcc gccttcatca taaccgccct cgcattgatt ggctgctgta
    24781 gctccgcccc ataaaaaatc ttttctgaat cccatcatac ttcctccatt acatgtgtat
    24841 tgcttgataa ttctcttgcc agttcatctt ggacttcgat tccttctgct tttaaccggc
    24901 tgattgtttc cttgaattgc ggtaaatact gctgatgtgc aataaacaac tcatccatta
    24961 tccgttttgc tgtctttcct gatcgtataa gcggattgat cgtaaacgct tgcaaagctg
    25021 ttccataatc accagtcacg gctgcttcga tcgtcagcaa ttccatattc ttcattactt
    25081 gcaaccagcc tctttcagca gcaggaaggg aaccaaacgc tacatttcgt gcgccttgtg
    25141 cgccaacata ggcagtgact tctactacac aatctgccgg cagatcagga acagctccat
    25201 cattctttgt agataccacg atctgtgttt ctttatttcc atagatcgaa gcaatcgttt
    25261 cacaggcagc atctgaataa taagcaccac ctcgtttttg caattgttca ggtttatgat
    25321 ccaattctgg atctttgtat aattcaaaca gctctgcttc tgtttctttc acttgctgcg
    25381 ctcttgtacc aatcgtttga tattcttcta aagcatgttc cagcatttct tcttcacggt
    25441 agtaatagcg gtggtagcca caaggaatca tgttcatttg ttttaattgt tctttgaaaa
    25501 acgggatatc aaagatattt ttcggcaatc cattgtcttc atcgtataaa cgatcaatca
    25561 attctgccgt gcggtcattt cctttattat ctgctacttt gtgccaatgg aagtgattta
    25621 gaccggcaaa cttgtagatc agctcttctt ttttcttgcc aagcattgtt ggttctgtca
    25681 tcatcgccat tactggcaca ttgcataatc cgatcacttt atcccatttg ccatatctga
    25741 taactgcttc tgtcaccatc ccgcttgggt tcgcgaaatt gatcagccaa gcatttggac
    25801 ataaacgctt catgtcttca acgattgcca aaaccactgg aacggtacga aaagctttga
    25861 atatcccgcc agcaccattt gtttcttgcc caatcatccc ataataacta gggatccgct
    25921 catctttgat tcgtgcatct aattgaccga cacgaaactg agtcgtaaca aaatccgcat
    25981 ctttcaatgc ttcttcccga tccagagttg catagatttt gacttcatac ggagaagcat
    26041 cccacatacg ctgagacatc tgactgacga tctcaagctt ttcttttcct tcttctacat
    26101 ctacgagcca gatttctcgt atcggtaatt cctcatatct tttgataaat ccttccatca
    26161 actcgggggt gtaactgctg cctccaccaa tcgtagcgat tttgattcct tttgccatgt
    26221 tctcactcct ttacttttta cacttttatt atcttttttg taaacaaaac aaacaataga
    26281 aattcgtttc tttccagtaa catttacaaa agttagtgtt ttcttctgga tttttgtaaa
    26341 tatcttgtca aaaaatgtct ttattaagct atataacata aatccgttta caaatcattt
    26401 tcaaaaaagt tataatttac gtaaaaaaac aagaagaagg tatctacatg ttattacgtg
    26461 aaaaaatcaa agaaatctcc ttttctcctt cagaaaatga agtgattcag tttctcttaa
    26521 gcgattctcc agcacttggc gaaatgacca tccaagagat tgctgagcga tgttatgtcc
    26581 acccgtccac actcatccgt gtagcaaaaa aaatgggttt tgatggctgg gtcagttttc
    26641 gtaatgcttt tttagctgaa acagcctatc ttgaagggaa ttttcaagac gtagatgcga
    26701 atcttccttt ttccaccaca gacggtatta tgacgatcgc aaataaaatg gcttctttag
    26761 aacagatgac gattcaagat acactttctc tcattcatca tgaccaactg caaaaagcca
    26821 aacaactaat gcttcaagca aaaaaaatcg tgattttcgc cagtaactcg aatactttga
    26881 tttctcaaga tttcatgctg aaaatgaagc gcatcaaaat ggatgtcacg attgtcacca
    26941 cttttggtga atcgttctat gaagcgtaca attgcacgag tgatatgtgc gggattctga
    27001 tttcttatac gggtgaaaac aatgtgatcc ttcgtaccgc agaaatattg aaacaaaatc
    27061 atgtgccaat tttagctttg accagtatcg gtgattcaac gctatcttca atgagtacag
    27121 ccgctttgcg acttacgaca cgcgaacgac tgtattcgaa aattggtaat tttacgatca
    27181 atacatcgat ttgttatctt ctagacgtgt tatatagttg tgtttttgca gaaaattatc
    27241 aacagaattt agtccatcta atttctgtag gacaaaaatt tgataaacgc cctatcagct
    27301 cagccatcat gaaagagcct ttcaattttg aaaataaaga agtggactaa aaagaataat
    27361 taagcctagt ccattctttc acctaaccgt tcaggtcagt gcggtgataa gtgcatcgtc
    27421 tggcccgacc gtctcccctt gattttccaa aatatccaaa taatccgtgt aattggtgac
    27481 gatcacaggt gtttctaaac tgtatccttc ctcttcgatt ttcttcttgt cgaagcggag
    27541 caacagatcg ccttgtttca ctctttctcc ttgctgcacc aagcttttaa aatattttcc
    27601 ttctaactga acagtatcca atccgacatg aatcagcaat tccagacctg cttcagaaac
    27661 cattccgatt gcatgtttcg tcgggaacag ggtcatgact gttccgtcaa aaggggcacg
    27721 tacttctcct ttttcagggt agatcaagat tcctcgacct aatgttcctt gtgcaaaagc
    27781 tgcatccttt gctgcattca gtggagcgat ttgtccttgt atcggtgttt ttacaatttc
    27841 tttacctgat gttttctgtc tatcatctgt ttcatctgct tcctccttcc aaaagaaaaa
    27901 agtcagcgca aaggatacca ccatcgcaat ggcgatccca atcaatacat taatcacatc
    27961 tgttaaatta ccatctggtc taatatagtt gggaattgta aagatgccta gtccaccttg
    28021 attccaagaa gttaaattca ttaacccact atacgcgccc cctaacgcac caccgacaca
    28081 ggtaaaaata aatatttttt tacgtggcaa tgtaaaccca taaatagctg gctctgtaat
    28141 ccccacaata ccagaaacaa tggctggtat tgcaattgct ttgtcttttg gatttctaag
    28201 cttaaagtag agtgcagtaa gtgctgcagt ttgtccaaaa gacactactg caacaggtgt
    28261 catgatattt tgccagccat tcacagaaaa ttgcatcaat cccattggta cgattgccca
    28321 atggagacca aacatcatta gaatttgcca gaagaagcct acgattgctc cataaataac
    28381 tggcgaaagt gctaaaagtg tttcaaatcc agccattaaa atatcagttg cgaatgtgaa
    28441 tgctggtcca acgagtaaaa aacctaaggg cattgtaata agaacggtga agaatggaac
    28501 gaagaaattc gcgatcatct caggtactaa ctttttaatt ggtttctgaa tttggctagc
    28561 taacaacaca ataaagataa tgggaatgac agaagaagcg tagttacgcg caatccaggg
    28621 aatgccgaat actgtttgat aaataggtgc ttcaaaaata gtgccagaaa atatggtcgt
    28681 cagtggtgct tctgctgcac cactcaaagc ttccagcgac aagctaggat tcattaatgc
    28741 tgccccaagc aacatcccta cgaattcatt tactttaaat ttacgggcag ctgttaaacc
    28801 gataataaca ggcataaatt ggaatacaga atcaccgata ttatcaaaca taacaaatgt
    28861 gccagaaccg cggtcaaatg cgcctaagaa gacaagtaat gctgtcagtc cacgaatcat
    28921 cccaccagca gctagaatag caaggaatgg ctggaaacag cccgatattc catcaataag
    28981 catatcaaac ggtctcattt tttctgttga aacagcctca tcaaaagtaa ggtttcctgc
    29041 tcttataacc tcttcaaaca cgactggcac atgattcccg atgacaactt ggtactgtcc
    29101 tcccgctcgc attaccgtca caacaccgtc catatttttc aagatctcat catttgcttt
    29161 ttcttcgttt tttaatttga aacgaagcct tgtgatacaa tgtgtcaaac cattgatatt
    29221 ttccttcccg ccgacatttt ctacaatttt gctcgctaat tcttgatatt ttcccatttc
    29281 tattccctcc aatttattag tggaaggttt agccaactga atgtcactac ccttatttca
    29341 tgcgttcttg ttaatttatc ctcctttttt atttatttac aacgattctc atgcactaaa
    29401 cgcgcagtat ggatcgttaa atacattttt tcgtccgtgg acatctggta gttgaatatc
    29461 tcggctaaaa agtctgctat tttctcaacg caaccaaacg tatccttata ttttttttga
    29521 ataacttggt acaattcctc agaaacttct cccacttctg atttttctcc atctaggacg
    29581 cgtgtcagaa aaaaacgtag gtgtgtgacg aatcgaaaaa aatggactga atcttcatca
    29641 aatgtaatct tatagtaata gcggacgata tttagaatat cctgtattgt tgtagtaaac
    29701 aggtaggtat tgtcttgttg gctttgaatg ccttgtgcat tgactaaatg caatgcgata
    29761 aatccggctt catcttctga taacttgatg tggtatttat ccgagatttt cttcactgtt
    29821 ttttccccga tggcatactc tttaggaaat agttgtttga tatcatacag caatacattt
    29881 ggtacaaata ttcccatttt gtgacgttcg atagccgtat acaaatgatc cgtcaatgca
    29941 atatacactg aatcattcaa ctggacttgt agagcctctt ttgtatattc aatgatttca
    30001 caagaaattt ctaagtactc aagaggaatc gcttgtgcca attcttggaa ctgttctgaa
    30061 agtccgtcat ttgtcaactg aaattttttg tcgatgcatg agtcaaaaat cgggtcaccc
    30121 actttctttt tgaatgccaa accttttccc atcacgatca tttcctgatt tctttcatct
    30181 ctggttatta cgacgttgtt attcagtact ttctcaatca gcatgtttta ctcctttcca
    30241 tgatatatag ggctgtatcc aaaacaaaaa gaaaaaccca aaaagccgtc catggaacca
    30301 tgggagactc atcaggttta gcttgcatca ggcaatcact atccaagttg tatcgttttc
    30361 tttatattat cgaaagcaag cgcttttgtc aacgctttct tagtggctat taggcacttc
    30421 tgactagctg tcgaatcttt tttaaatctg gtgactacga cactaccgct cacagatgaa
    30481 gaatctggga agctctgttt tattttcact ctagcgctta ggtcaaaatg ctactatgct
    30541 actgtcccaa cttctaatat acggtgttcc agtatcagct tatcggtgat aagccaaatt
    30601 tatgcaaaaa tctggaaagc cttttttaca atttttttct tatcacctaa agctgaacgg
    30661 agccctgtca caaccacttt ttacacacca ctgctttgca gtttgtacat atctgcatac
    30721 aaaccatttt gcgctagaag tgtttcatga ttcccgcgtt ctactatatg acctttgtct
    30781 aaaactaaaa tcaattctgc atcccgaatc gtggacagcc gatgggcaat cgcaatcgtt
    30841 gtccgtcctt gtcgcatttt tgtcaatcct tcttggatca atccttctgt ttcagtatcg
    30901 atatttgctg tcgcttcatc caatactagt attttaggat ctgtcacgat tgttcgcgca
    30961 aaagtgatca gctgtcgttg tccgctagaa tagctggcac cgtgttcgat cacttttgag
    31021 tgatatcctt ttggtaattc atcgatgaac ttgtttgctt gaacaaactc agctgcatgg
    31081 cgaatctgtt cgtctgtgat agttggattc atcatccgga tatttcctgc gatatctcca
    31141 taaaacaaga aggcatcttg caagaccaat cccattttct tacgtaactc ttccattgga
    31201 tattctcgaa tgtcatgatc atcaatcaag atctggcctt cataaaattc gtaaaaccgc
    31261 atcaatacgt tgatgatcga gctttttccg cttccggtgt gccccactaa tgcaacggtc
    31321 tctcccggat tagcgacaaa agagatattg ttcaatacat tgtgctctcc atcataagaa
    31381 aagcttacat tgcgaaactc gatcttacct gaagtaatcg tttgatctgc tccaggattt
    31441 tgttgcggtg catattcttc tgtatccatg attttcaaaa tacgactacc ggcaaccatt
    31501 ccatctgtaa aggtgctcaa aaagtccatc atttgagaca ttggattaaa aaatccctgg
    31561 acataggtcg taaatgcata gatcagtcca acttctacgg gagaatgcag cgcatcgatc
    31621 ccaaacaaag tcaacgccaa cgcaatcgcc aatgcatata gaaaattgat gatcggactt
    31681 aacagaatcg aattcgtttt gatcatcgca tagcgcgtgt ctaaatactc gttgttcact
    31741 tgttcaaatt ctttagctaa acgagcttct tggcgaaact gctggataat acgcatgccg
    31801 gaaatcgatt cattcaattt cgtgttcagc tgacttaatt tttcccgcat attccggtaa
    31861 atacgagaac tgaatttctg gtaataccag atgacgattc ctaaaatcgg caagaacacc
    31921 aaagtccaca aggcaatttc tccattgatt tgaaacatgg caatgaagga agaaacgact
    31981 gcaaagatcc cagtcaatac catcaaaaag acataccaaa attcaaatag cgtttctgtg
    32041 tcatttgtca ctcgagaaac ggtcgatcct gccggtgtct gatcaaaata acgcatacct
    32101 agtgtatgca gcttttcaaa taatcttacc cgaacatact gataggtctt caatgaagcc
    32161 atggaataca aataccattg gaaaaaccaa atgatacttt tgactatgac tccaaacaag
    32221 tacataccag caaaaaacaa aataacttgt gtcgtagccg actgtttagc cagatgatca
    32281 tccataaaag tttggatgat ccttggcaat aggacattga tcacagacag agcaaacgca
    32341 aataagatag caacgatgaa cgttcttcta aaaggtttgg cgaacttcat cagtcgtttg
    32401 atgatcttct tctgttcttt aagtggaatc gattttgtcc attccgattg ataattttca
    32461 tccatcaggc ctcgcctcct tctatcttcg cttctaattg ctgtttctcc cacattcgtt
    32521 cataccagcc gcctaatgca agcagctcat gatgggttcc tcgttcaatg attttccctt
    32581 gatcgacaac aatgatttct tttgcatgca tcacgctgct taaacggtga gctgcaatga
    32641 tggttgtttt ctcttttctt atttctttta aattatgcaa gatagcttct tctgttttgg
    32701 catctactgc agaaagtgca tcatctaaga tcagcaattc aggatttttg atcaatgctc
    32761 gagcaatcga aatcctttgt ttctgtccgc cagataaaga gactccacgt tctccaacta
    32821 aggtatcata accttttggc aattccttga tttcattgtt gataaatgct aaagaagccg
    32881 catgctctac ttcttcttct gaaagttttg gattagaaaa acggatatta tctcgaatgg
    32941 tcatcgaaaa caagaagtga tcttgaggca catagccgat tgatcctaat aaagcattca
    33001 atgaatattc tttgatcgaa cgtccgccaa attggatttt tccttgataa tcatcgtatt
    33061 cacgcatcag taatttgatg atcgtcgttt ttcccgcacc agttttccca acaataccta
    33121 atgtttctcc ttcttggatc acaaatttga tttgttctaa tgctggtgat tcttctttcg
    33181 gatagttaaa agaggaaata gctacattca gctcgccttt tgccggggtc tgcactgcac
    33241 ccagttgttc cacgatatgc gtcttttcat gcaaaagttc attgacgcga tcataagaag
    33301 catttcctct ttccaatacg ttgaacaatc gaccaatcgc aaacatcggc caaacaagca
    33361 taccgatata gctaataaat gagaccatct gcccgatcga gatcgtgcct tccaagacat
    33421 actttccacc taaaataatt gttgcaacat aagacagacc gataatcaag gtaatgaatg
    33481 gatcaaataa ggagtctaaa aaattgactc gtttgttttt aataattgca tcttcaatct
    33541 tttcttcaaa atccgcgaga tcttcttttt cttgtccaaa tgttttaatg actttgattc
    33601 cagtaatact ttcttgtgtc ttatcattga tactagaaaa agcttcttgc gaatcacgaa
    33661 aagcatcgtg gagttttgac cccagcactc gtgaagtaac ggctagtaaa ggcagaggaa
    33721 tcagcgcaat cagtgttaag cgccaatcaa taaacaaaat catcgcaatg atcgttgttc
    33781 cgcccgtgat cactgaatct gcaaaggtca agataccagc tcctgctaca ttttggattg
    33841 cattcaaatc attcgttgca tgagccatca aatctccagt ccgatatttt tggtaaaaga
    33901 gattgtccat ttctgtaaaa tgtttgaata gctgctgccg aagttctttc tccaaccgag
    33961 ctgcacttcc ccaaatattc gtccgccaga tataacgaaa aagatattgt aagagagctg
    34021 caaggatcaa taataagatc caccaggaaa tgatctttat tgataaattt ttatcggcaa
    34081 tctcatcaat gatgatccca atcacctttg gaggaactaa ctgtaccaag gccactgcga
    34141 atagggagaa aatcccaatt aaataatgtt tcttttcctg tcgaaaaaac caacctaatt
    34201 ttctaaaaat cgacatttta ctcctccttt gtctttatca agagcatagg attgtctgtg
    34261 cttgtctttt ttgctgaata gtctgaatag tctaacaaat tacgaaataa aaaacggact
    34321 gaaaaaatca gcccgcaaaa agtctttggg tcagaaccta ttttttccct tggttcttca
    34381 tttgctgcat catctggcga attttcttct cagatggttt ttgccccatt gacatcatca
    34441 ttgatcgcaa catgtcctca ttaacgggag gatttttttc taaatagtct ttcatatatt
    34501 tacgcgctag gaaaaagcca cctactgcgc cgattagcaa agcaataata gcaataagta
    34561 cgacaatacc tgttgatacc atataaatct ctcctttcta tttccgaatt gctctcaatg
    34621 atttatttta ctaaagttta ttgaaaaaag aaaggcgtat tctttagttt catgcaaaaa
    34681 atagtggttt ttttagagaa gaacaaaatt tctttcatta accagcaaga cacgccacga
    34741 aaaaaaatta ggcataccga atatttttct ttaatttttt ctttctgagg tgtagaatgt
    34801 aatcgtagat aaataggaaa ggtggttttt tcttgaataa tcaatccaac aaacaatttc
    34861 ataagcatcg acttattgaa catacaaatg gtttatcttt agctgaagta aatggaacag
    34921 tggatgtacc caagggaaaa agttttttta aaacacttct tgcttactct ggaccaggtg
    34981 cattagtagc agtgggttat atggatccag gtaactggtc cacttcgatc actggtggac
    35041 aaaatttcca atatttattg atgtcagtta ttttgatgtc tagtctgatt gctatgcttt
    35101 tacaatatat ggcagcaaaa ttaggaatcg tgacccaaat ggatttggcc caagcaatcc
    35161 gagcacggac aagtaagaca ttgggtgtca ttttatggat cttgactgaa ttagccatta
    35221 tggcaacaga catcgcagaa gtcattggtg cagcaatcgc cctctacctg ctttttgata
    35281 ttccattagt gattgccgta tttatcactg tgttagatgt tttcgtcctc ttattgctga
    35341 caaaagtcgg gtttcgtaaa atcgaagctt tggttgtctg cttgattttc gtcattttat
    35401 tcgttttcgt ctatcaagtt ggcttgtcta accctgattg gcacagtttg tttgccggct
    35461 ttatccctaa cagtcataca ttctcagact caccggtcat cggcggtgaa actccgctga
    35521 ctggagcttt agggattatt ggtgcaacag tcatgccgca taacttatat cttcattcag
    35581 cagtttctca aactcggaag atcgatcacc aagatgaaga agatgtagca caagcggtac
    35641 gcttttctac ttgggattct aatatccagt tgtcaatggc ttttctagtc aatgcactgc
    35701 tattgatcat gggtgtcgct gtattcaaaa ccggttctgt ccaagatcct tccttttttg
    35761 gtctgtatga agctttatct gatccatcag ctatgagcaa tggtatcttg attgctgtcg
    35821 caaaatctgg tatcttgtcc actttatttg ctgtagcact tcttgcttct ggccagaact
    35881 ctactattac tggtacgttg accggtcaag tcatcatgga aggcttcatc catatgcgta
    35941 tcccgatttg gctaagaaga ctagtcacac gattgctttc tgtgattcct gtattgattt
    36001 gtgttttgtt tacaagtaca aaaggaacga tcgaagaaca taccgctttg aataacttga
    36061 tgaatgaatc ccaagttttt ctggcttttg cattgccatt ctcgatgatt cctttactga
    36121 tgatgaccaa tagccaagcg gagatgggga gccgattcaa aaattcattt gttgtaaaac
    36181 tacttggctg gttctctgtg attagtctga cttacttgaa cttacgagga ttaccaggtc
    36241 aaatcgaaag tttcttcggc gagcaagcca cctcagctca actagcattg gctgatcaaa
    36301 tcgcttatgt aatcattgtt cttgtccttc tcctgttggc atggacgatt ggtgatttat
    36361 ataaaggaaa caaaaaatat gaacagcgtt tgcaagaaac gatagaagaa atcgaataga
    36421 aactgattct tttacacaaa ggaggttgtg acagaagtgg gcagcttcaa gaaataagaa
    36481 ggaatttaag aaaattgctt ttcaaatttt tgtgaaattt cggcttattt ttcgaagaag
    36541 ttacttctgg aacaccgtgt atcagaggtt gtgaaaaagc tg
//
LOCUS       DACCCS010000022        36570 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.022, whole genome shotgun sequence.
ACCESSION   DACCCS010000022 DACCCS010000000
VERSION     DACCCS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.022"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IVK82_001174"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IVK82_001174"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP6392248.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IVK82_001175"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IVK82_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP6392249.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IVK82_001176"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IVK82_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP6392250.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IVK82_001177"
     CDS             complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IVK82_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP6392251.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IVK82_001178"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IVK82_001178"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP6392252.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IVK82_001179"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IVK82_001179"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP6392253.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="IVK82_001180"
     CDS             complement(8054..8524)
                     /locus_tag="IVK82_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP6392254.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="IVK82_001181"
     CDS             complement(8570..8995)
                     /locus_tag="IVK82_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP6392255.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="IVK82_001182"
     CDS             complement(9062..9331)
                     /locus_tag="IVK82_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP6392256.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="IVK82_001183"
     CDS             complement(9342..10937)
                     /locus_tag="IVK82_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6392257.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IVK82_001184"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IVK82_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP6392258.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="IVK82_001185"
     CDS             complement(14490..15050)
                     /locus_tag="IVK82_001185"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP6392259.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="IVK82_001186"
     CDS             15403..16377
                     /locus_tag="IVK82_001186"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP6392260.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="IVK82_001187"
     CDS             16547..17947
                     /locus_tag="IVK82_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP6392261.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="IVK82_001188"
     CDS             complement(18025..18747)
                     /locus_tag="IVK82_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6392262.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="IVK82_001189"
     CDS             complement(18826..19332)
                     /locus_tag="IVK82_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6392263.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="IVK82_001190"
     CDS             19455..21254
                     /locus_tag="IVK82_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP6392264.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="IVK82_001191"
     CDS             complement(21322..22896)
                     /locus_tag="IVK82_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392265.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IVK82_001192"
     CDS             complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IVK82_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP6392266.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IVK82_001193"
     CDS             complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IVK82_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP6392267.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24673..25311)
                     /locus_tag="IVK82_001194"
     CDS             complement(24673..25311)
                     /locus_tag="IVK82_001194"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP6392268.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25312..25962)
                     /locus_tag="IVK82_001195"
     CDS             complement(25312..25962)
                     /locus_tag="IVK82_001195"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP6392269.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IVK82_001196"
     CDS             complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IVK82_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP6392270.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IVK82_001197"
     CDS             complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IVK82_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP6392271.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29105..29845)
                     /locus_tag="IVK82_001198"
     CDS             complement(29105..29845)
                     /locus_tag="IVK82_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP6392272.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IVK82_001199"
     CDS             complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IVK82_001199"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP6392273.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31216..32154)
                     /locus_tag="IVK82_001200"
     CDS             complement(31216..32154)
                     /locus_tag="IVK82_001200"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP6392274.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32147..32638)
                     /gene="def"
                     /locus_tag="IVK82_001201"
     CDS             complement(32147..32638)
                     /gene="def"
                     /locus_tag="IVK82_001201"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP6392275.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IVK82_001202"
     CDS             complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IVK82_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP6392276.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35314..35619)
                     /locus_tag="IVK82_001203"
     CDS             complement(35314..35619)
                     /locus_tag="IVK82_001203"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP6392277.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IVK82_001204"
     CDS             complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IVK82_001204"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP6392278.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7989 c         6707 g        11988 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt
    23221 gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc
    23281 aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat
    23341 tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa
    23401 gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg
    23461 agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat
    23521 gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt
    23581 cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc
    23641 attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact
    23701 gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag cccataacta
    23761 tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga
    23821 ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc
    23881 actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc
    23941 gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt
    24001 ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact
    24061 gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa
    24121 ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga
    24181 ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt
    24241 gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc ataagttgaa
    24301 aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt
    24361 ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca
    24421 ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag
    24481 gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag
    24541 actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta
    24601 agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta
    24661 tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa
    24721 ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt
    24781 attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca
    24841 ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata
    24901 agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg
    24961 ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat
    25021 caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc
    25081 tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt
    25141 ctgctcttcg ttcgacaatg aatcaaaatc gcccactgcc agatctactg ggtagcctgc
    25201 ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag
    25261 agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa
    25321 tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc
    25381 tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc
    25441 gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt
    25501 aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat
    25561 gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg
    25621 attgataact actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat
    25681 atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg
    25741 aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg
    25801 acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatt ggccatccat
    25861 tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa
    25921 attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt
    25981 tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg
    26041 caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc
    26101 gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac
    26161 aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct
    26221 aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt
    26281 tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg
    26341 tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag
    26401 ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac
    26461 cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga
    26521 caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag
    26581 aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc
    26641 aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag
    26701 aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt
    26761 gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta
    26821 tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt
    26881 ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa
    26941 gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat
    27001 caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg
    27061 ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg
    27121 gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga
    27181 tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc
    27241 cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca
    27301 tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag
    27361 ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt
    27421 tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca
    27481 gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc
    27541 caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt
    27601 cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat
    27661 ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga
    27721 atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta
    27781 ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg
    27841 cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg
    27901 attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt
    27961 gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc
    28021 ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg
    28081 atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct
    28141 ggcaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc
    28201 attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc
    28261 tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt
    28321 tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt
    28381 tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa
    28441 tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc
    28501 tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg
    28561 gcctgctcag gggacagata atgaacagat cccagcatcg tattcgtctg cgtgatcgat
    28621 gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg
    28681 atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa
    28741 atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt
    28801 gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg
    28861 ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct
    28921 gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt
    28981 aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc
    29041 ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac
    29101 tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc
    29161 ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat
    29221 tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca
    29281 gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca
    29341 tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga
    29401 tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt
    29461 tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat
    29521 ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc
    29581 cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt
    29641 tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct
    29701 gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga
    29761 gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta
    29821 catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa
    29881 tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg
    29941 atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca
    30001 agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca
    30061 gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg
    30121 gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca
    30181 gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact
    30241 gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac
    30301 gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt
    30361 ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg
    30421 cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg
    30481 aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc
    30541 cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt
    30601 cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag
    30661 catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat
    30721 ccaccagtcg tttcgtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag
    30781 gatcagcaat gccttgcaga tctggtgctc cttgacgctg ataattcctc aagacaccat
    30841 ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta
    30901 aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa
    30961 gcgtcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt
    31021 ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt
    31081 tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac
    31141 ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt
    31201 tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt
    31261 aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa
    31321 gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc
    31381 aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc
    31441 ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc
    31501 ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt
    31561 tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc
    31621 tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg
    31681 tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat
    31741 ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc
    31801 acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac
    31861 ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac
    31921 caattcaatg atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg
    31981 gataccgtgc ttcagagccg cttcttttac cggtgtcggc gtaattactc gtttccgccc
    32041 aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc
    32101 ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat
    32161 gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga
    32221 acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag
    32281 ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc
    32341 gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac
    32401 cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga
    32461 tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg
    32521 caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga
    32581 ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa
    32641 tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg
    32701 tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc
    32761 atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct
    32821 gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat
    32881 ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt
    32941 aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg
    33001 ctcataattt tgttttttag ctaattctat ggcgtaatgt tgaggattga atgtttggat
    33061 cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg
    33121 gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac
    33181 cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa
    33241 tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg
    33301 agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc
    33361 ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca
    33421 gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat
    33481 atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca
    33541 catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc
    33601 taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg
    33661 aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg
    33721 gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga
    33781 gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag
    33841 atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc
    33901 gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac
    33961 tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag
    34021 tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg
    34081 cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc
    34141 agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa
    34201 gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc
    34261 tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata
    34321 acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt
    34381 actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca
    34441 caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat
    34501 ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg
    34561 gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct
    34621 gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac
    34681 ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg
    34741 aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc
    34801 taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt
    34861 caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg
    34921 acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat
    34981 ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat
    35041 gacttcagct gttaatgtca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg
    35101 acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa
    35161 tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca
    35221 cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct
    35281 tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg
    35341 tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt
    35401 caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc
    35461 ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact
    35521 tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac
    35581 acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca
    35641 ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt
    35701 tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca
    35761 tcatttcgat ttcttctttt gcaactttca ttcgttcttc gattacttca tctgcatctg
    35821 ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga
    35881 atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga
    35941 aaacatcttt tccttcatcc aatgtcttgt tgatatatga caaaggagtt ccataataat
    36001 tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt
    36061 ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag
    36121 tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg
    36181 tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga
    36241 cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa
    36301 ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga
    36361 ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc
    36421 atgtttttcg tttggttggg gttattatcg cagatgcaag cttttgaacg ccgtgtataa
    36481 gaggtcgtga agaagccgat tagtttcgag tatgaagaaa aattttgtga aaatagtttc
    36541 tcacattttt gcataaattt ggcttaatac
//
LOCUS       DACCCS010000023        36564 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.023, whole genome shotgun sequence.
ACCESSION   DACCCS010000023 DACCCS010000000
VERSION     DACCCS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.023"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            6..980
                     /locus_tag="IVK82_001205"
     CDS             6..980
                     /locus_tag="IVK82_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP6392279.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            1710..2027
                     /locus_tag="IVK82_001206"
     CDS             1710..2027
                     /locus_tag="IVK82_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392280.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(2231..2596)
                     /locus_tag="IVK82_001207"
     CDS             complement(2231..2596)
                     /locus_tag="IVK82_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392281.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            2804..3286
                     /locus_tag="IVK82_001208"
     CDS             2804..3286
                     /locus_tag="IVK82_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392282.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            3483..4373
                     /gene="pstS"
                     /locus_tag="IVK82_001209"
     CDS             3483..4373
                     /gene="pstS"
                     /locus_tag="IVK82_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP6392283.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            4698..7169
                     /locus_tag="IVK82_001210"
     CDS             4698..7169
                     /locus_tag="IVK82_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6392284.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7272..9008)
                     /locus_tag="IVK82_001211"
     CDS             complement(7272..9008)
                     /locus_tag="IVK82_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP6392285.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9005..9709)
                     /locus_tag="IVK82_001212"
     CDS             complement(9005..9709)
                     /locus_tag="IVK82_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6392286.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            9840..10343
                     /locus_tag="IVK82_001213"
     CDS             9840..10343
                     /locus_tag="IVK82_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP6392287.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10303..10641
                     /locus_tag="IVK82_001214"
     CDS             10303..10641
                     /locus_tag="IVK82_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP6392288.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10662..12080
                     /gene="ffh"
                     /locus_tag="IVK82_001215"
     CDS             10662..12080
                     /gene="ffh"
                     /locus_tag="IVK82_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP6392289.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12192..12770
                     /locus_tag="IVK82_001216"
     CDS             12192..12770
                     /locus_tag="IVK82_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP6392290.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            12774..13295
                     /locus_tag="IVK82_001217"
     CDS             12774..13295
                     /locus_tag="IVK82_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6392291.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13374..13928)
                     /locus_tag="IVK82_001218"
     CDS             complement(13374..13928)
                     /locus_tag="IVK82_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6392292.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            14181..14456
                     /gene="rpsP"
                     /locus_tag="IVK82_001219"
     CDS             14181..14456
                     /gene="rpsP"
                     /locus_tag="IVK82_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP6392293.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            14468..14719
                     /locus_tag="IVK82_001220"
     CDS             14468..14719
                     /locus_tag="IVK82_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP6392294.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            14844..15635
                     /locus_tag="IVK82_001221"
     CDS             14844..15635
                     /locus_tag="IVK82_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392295.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            15805..16326
                     /gene="rimM"
                     /locus_tag="IVK82_001222"
     CDS             15805..16326
                     /gene="rimM"
                     /locus_tag="IVK82_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP6392296.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16316..17077
                     /gene="trmD"
                     /locus_tag="IVK82_001223"
     CDS             16316..17077
                     /gene="trmD"
                     /locus_tag="IVK82_001223"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP6392297.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17213..17560
                     /gene="rplS"
                     /locus_tag="IVK82_001224"
     CDS             17213..17560
                     /gene="rplS"
                     /locus_tag="IVK82_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP6392298.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            17933..18595
                     /locus_tag="IVK82_001225"
     CDS             17933..18595
                     /locus_tag="IVK82_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392299.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            18674..21901
                     /gene="fss3"
                     /locus_tag="IVK82_001226"
     CDS             18674..21901
                     /gene="fss3"
                     /locus_tag="IVK82_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP6392300.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            22015..22329
                     /locus_tag="IVK82_001227"
     CDS             22015..22329
                     /locus_tag="IVK82_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6392301.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            22342..22716
                     /locus_tag="IVK82_001228"
     CDS             22342..22716
                     /locus_tag="IVK82_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6392302.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            22726..23070
                     /locus_tag="IVK82_001229"
     CDS             22726..23070
                     /locus_tag="IVK82_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP6392303.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            23141..24493
                     /locus_tag="IVK82_001230"
     CDS             23141..24493
                     /locus_tag="IVK82_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP6392304.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            24553..25695
                     /locus_tag="IVK82_001231"
     CDS             24553..25695
                     /locus_tag="IVK82_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP6392305.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            25720..25974
                     /locus_tag="IVK82_001232"
     CDS             25720..25974
                     /locus_tag="IVK82_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392306.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            26230..27414
                     /locus_tag="IVK82_001233"
     CDS             26230..27414
                     /locus_tag="IVK82_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6392307.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            27429..27548
                     /locus_tag="IVK82_001234"
     CDS             27429..27548
                     /locus_tag="IVK82_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP6392308.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            27553..27774
                     /locus_tag="IVK82_001235"
     CDS             27553..27774
                     /locus_tag="IVK82_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392309.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            27787..28287
                     /locus_tag="IVK82_001236"
     CDS             27787..28287
                     /locus_tag="IVK82_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP6392310.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            28384..30231
                     /locus_tag="IVK82_001237"
     CDS             28384..30231
                     /locus_tag="IVK82_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP6392311.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            30234..31130
                     /locus_tag="IVK82_001238"
     CDS             30234..31130
                     /locus_tag="IVK82_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6392312.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            31180..31569
                     /locus_tag="IVK82_001239"
     CDS             31180..31569
                     /locus_tag="IVK82_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6392313.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            31556..33058
                     /locus_tag="IVK82_001240"
                     /pseudo
     CDS             31556..33058
                     /locus_tag="IVK82_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            33803..35629
                     /gene="ltrA"
                     /locus_tag="IVK82_001241"
     CDS             33803..35629
                     /gene="ltrA"
                     /locus_tag="IVK82_001241"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6392314.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            35763..>36564
                     /locus_tag="IVK82_001242"
     CDS             35763..>36564
                     /locus_tag="IVK82_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6392315.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT        12330 a         6156 c         7723 g        10355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta
     1381 gagtaattaa ttagatgttt attttatagg actgatatta tagattacaa atagtatcgt
     1441 atttcgcaat cttaaaatta tcttcttact atgaataaga taataaaatt tgtatttgtc
     1501 ctttttaggt aacttatttt aatctatata cctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatggt aactatgcac tgtaacgata atgctgctaa aatatctttt
     1621 tacgaaaaac atcacaagga aaaacttaac tatggtatta tctaaacata attttttcgg
     1681 tatttaatat ttaattagga gggaatattt tgctttttta tagtaatttt atcttaatag
     1741 tagcgatatt attacttctg aatatatgga tttttgatag gagtagaaat gcaagtatag
     1801 gttttagaac taaacgaagt ctgtcttcta aaaaaaattg ggtatactca caaactattt
     1861 tttatggagg aattgtttta attagtttac tttcttcaac actttattct ttgaatatta
     1921 tagatgtttc cacctcaaat tcaatatcta ttattggaat cattattgct gcaattatta
     1981 cacaactgtt tttagtgttt ggagagaaga aaagaagtaa aaaatagaaa aactagtgat
     2041 agttttggtg atagtgatat taaaaatcag aaaagataga aaaagcagga gatagataaa
     2101 atgctgatat ttcagcattt tttaagagta taaaagagaa aaaaagtaat aaatgtatac
     2161 atgatataag aaaaacatat atgtataaaa agaagaaagt agcttctctt tgtacatata
     2221 tggtttgttt ttatggatgc cgagcttttt tataagataa ggctaccgcc cataacgacc
     2281 caaggatttg tggaactaag taaatacctg caagaagcaa gatcagctgg gtcggtttta
     2341 aaggatgata gaagtaacgc cattcagtaa aattggcgca aaataaatag gaagtgacag
     2401 tagatataat attggcaatg aataaagata taatacgcct gttcctttga aaataatatc
     2461 cagcaaaaag agataaaaat atcaatgtga tgaacccgct tacggaatga taacgaaaat
     2521 caagcagtaa ggcgaaatgc acataaggaa caaaagataa aagcaacgga aggtttttcc
     2581 agcaagtttt tatcatcttt catcaccacc taacaagtgt tattattttg ttagttttat
     2641 tgtaaattga atcctgcaat aatcaattac tttttcatta tttctagatt aaatgaataa
     2701 actctcatgc cattatctct aaaaaaaaag tttttccccc taattttttt agatagaaaa
     2761 ttgtatagta tgatgagaga aaaagtagtt tgggggaacg ttaatgagaa gaaaagcagt
     2821 aatcgatcgg ctttatttat ttttttggtt ttttcctatc gttttgctac cttttacatg
     2881 ggtaaatgct gttgtacaag gtgcggagat attaggcctg atccaaggaa gcggatttgt
     2941 ggtttctagg aattttccag ctttgactgt tttctattta gtgataagtg taggtctctt
     3001 attaccaaaa tctctgatca atcactacaa ttggcagctg ctttactttt cttgcttatt
     3061 agtatttaca tctatgtttt ctttgctgcc tcgcttgatt atcggcccta atggtttgag
     3121 cgagagtgga ttgaaggatt cagcttattc ctccatgtat ttcttgacga tcaaaccatg
     3181 gttctattta gtaattgcct catttattct tgcaacattt ttttacttct ggacagaaat
     3241 caggataaaa agtgaacgat ctgtatctat aaaaaataac tattgatggt ggatcatcag
     3301 taaaaaagca tatgagctgc gtaaaatgca tgtttctcat atgctttttt tattattatg
     3361 taaagaatgc gaaaaacgtt tacatgattt ttacataact taactatatt tttacagcaa
     3421 ccatacgagg gatttacagt cgactgataa actttagttg taaataagaa aggagcaata
     3481 aaatgaaaaa agtattgttt actctaggta tgatgggaat tcttttagcc ggatgcggct
     3541 caggatcagg aaatacagat ggctctgcca cacaaaataa ttcaagtgac tcaaatgaac
     3601 aagtaaaaat cgttgcggta ggttcgacag cattacagcc gttagtcgat gcagcacaag
     3661 aaagctttgt acaagaaaat ccaaattatc aaatctctgt acaaggagga ggaagcggaa
     3721 ctggtttgag ccaagtcgaa gctggagcag ttactatcgg aaactctgat gtatttgcag
     3781 aagaaagaga cggagttgat gcttcaaaat tagtggatca taaagtagca gtggtcggga
     3841 tggcgccaat cgtcaataaa gatacagatg tcaaagatat caccaaacag gaattgattg
     3901 atatctttac aggaaaaatc actaactgga aggaagttgg cgggaaagat caaaaaatca
     3961 acgtcgtgaa ccgtgcaaac ggcagcggta cccgcgcgac attcgaaaaa tggggactgg
     4021 atggtgctac ccctgtgcag tcccaagaac aagattcatc aggaacagtt cggcagctgg
     4081 taagtcaaac accaggagca atcagctatt tagctttctc atatctcgat gattccacac
     4141 aagcactaag tatcgacggt gtagaaccaa aagaagagaa tgtagcagat aacagctggg
     4201 gaatctggtc atatgaacat atgtacacga acggaaaacc ttcccctgaa gtccaaaaat
     4261 tcttagacta catgatgaca gaagaaatcc aagaaggacc tgtcaaagaa ttgggctatt
     4321 taccaatcac aatgatggaa gtagaacgtg atcacgaagg aaatatcaaa taactagcag
     4381 ataaacatgc cacggtagtc gttactatta aggaaaaatc aactatgttt cccacatata
     4441 catttttcct ttaattgtga aaaatagttc ctcatatttt gccgtttttt gagctaatac
     4501 cgagggtcag ctgattaaac accgtttatt cgaaaaaaag agctacgaca tagaactgtc
     4561 gtagctcttt ttttgctata tgacaggaat gaaaggtgtt tgcaagctta atgtgaggaa
     4621 cactttccat atctgttcta tactggatag gagaagtttg gaaatatcca gaaaacgaaa
     4681 tcaatcgagg tagccacatg aagaataaat ggataaatcg aagtaaaaaa aagaaagtaa
     4741 cacaaagatc atcagataaa gtcatccgtc tttttccatc gtataaagaa ggattaacgg
     4801 cagatcaagt caaggaacga atagaaaaag gtgcagcaaa taattcagtc gatcccacat
     4861 tcaaaacaaa tcagcaaatc gtattggaaa acatatttac gtatttcaat ttgatctttc
     4921 ttatcttagc cattttgctt tgtttagtag aatcctataa gaacttgact tttttaccag
     4981 ttatcatagc caatacagga atcgccattt ttcaggaaat tcgatccaaa aagatcttgg
     5041 acgaactaaa tgttttaaat acagcaaaag tcaatgtcat acgggacggg atagaacaaa
     5101 caatagatat agaagacctt gtaatagatg acattgttat tttagagact ggacatcaaa
     5161 ttccagcaga tgcagaggtc gtggaaggaa aactgcaagt taatgaggct ttattgacag
     5221 gcgaagcaga tgaaataacc aaagaaatag gcgatccatt aatgtccggc agtttcattg
     5281 tttccggaaa agcttacgcc cgattagaca aagttggagc agattcgtat atttcccagc
     5341 tgacagtcaa agccaagaca atgggagaag gagaacagtc ggagatgatt gcttctctta
     5401 atcaattgat caaatggatt gggatcatca ttattcctat cggtatcctg cttttttctc
     5461 aaagttactt ttttaatgga aacacgatca aagaaagtgt cgttgcaatg gaagcagcat
     5521 tgatcggtat gatacctgaa ggactgtact tgctgacgac gattgcatta gcattaagtg
     5581 cgactaaact agcaaagcaa agagttcttt tacacaatat gaaaagcatt gagacattgg
     5641 caagggtaaa tgtcctttgt gtagataaaa caggaacgat cacagaaaat aaaatgtctg
     5701 ttcaaaaagt gatcgtctct aaaaaacaag aaataatcag tcagtcagat caattggaag
     5761 catgcattgc tgactatgca aaagcgatgg caggagacaa tgcaacaatg gaagctgtta
     5821 aagcatattt tacaaaaacc actgatcaat cctatcacca tgttattcct ttttcttctg
     5881 tccaaaaatt cagcagtgta gcgttggcag acaaggtgta tgttttagga gcgcccgaaa
     5941 tggttttgcg cgatcaattg ccggaatatg cggaagagtt tgtttctttc gctgaaaaag
     6001 gctatcgcgt gttggtcttt ggtgtgtacg atgggattct tgaagaacca gtattgacag
     6061 aagcagtcac accattaggc tatattctga ttgctaatcc tatcagaaaa gaagcaagag
     6121 ccacatttga ttacttcaaa aaacagcaag tagccatcaa agtgatttct ggagataatc
     6181 cgctcaccgt atccaatgta gctgtccaag cagggatttc acatgctgat cagtacgttg
     6241 atgcatctaa attatcagaa gaagaatatg aagcagctgt cgaaaaatat acagtttttg
     6301 gacgcgtcaa gccagaacaa aagaaaatct tcgttcaatt gataaagcgt aacaatacag
     6361 tagctatgac aggtgatggc gtgaatgata ttctagccat gaaagaagca gactgcagta
     6421 tagcgatggc ttccggaaat gaagccgcca tgcaggcctc acaagtggtc ttacttgatt
     6481 ctgacttttc acgcatgcca gaagttgttg cagaaggacg gcgcgttgtg aacaatattg
     6541 agcgatcagc cagcttgttt ttagtgaaga atattttctc ctttttgctc tcactgtttt
     6601 cagtgatctt tgctctgacc tatcctttag aaccatcaca aatcacattg attagtttgt
     6661 ttactatcgg acttccatct ttcttgcttg ctctagaaga aaacaaaaag cgaatcagag
     6721 ggaaattcat catgaatgtg atggaaaaag ctgtgccagg tggactaaca gatatgatcg
     6781 tagtcggtgc gttggtgatc tgcggggtga ctttgaatct aaataaaaca gatgtttcta
     6841 cagcatctac catgcttttg attgcagtag ggttcctcgt tctctataaa atctgttcac
     6901 cgctgaataa attcagaagt cagatcattc ttttctgtgc tagcggtaac tttttttcgg
     6961 tcatcttttt gcataagctg ttttcaatca ccggtatctc agctgtatca cttttgctgt
     7021 tgagcattct atttttctcg gcagattcga tttttaggca tttgaccaca ttggtagaat
     7081 atttgtttga acgtaaaaat gaaaaagagc aaaagccgtt tagtcgcgta gctaaaaaat
     7141 ttctatcgat atttaaacga gaaaattgac gaaaagtatc tcaaaaaaga gtaaaaaaga
     7201 acaaccactc gtgtatcttt cgaagcagga tgagtcatcc aactttaaaa cgcacgagca
     7261 gttgctcttt tttacatttt cggaatggta acggtgaaag tcgaacctgt tccaagatga
     7321 ctgtctacag taatcgtacc attaaggagt tgcacgtagt cttttacaat cgctaaccct
     7381 aatccggtcc cgcctgaatg tcggcttcgc gccttgtcta cgcggtaaaa acgctcaaag
     7441 atccgttcct gatcctcaat gtcgatacct ataccaaagt cttggacaga aaacgataga
     7501 tcatctgttg cctgatagcg gataataatc tcgctgtcgc tttttgaata ttggacagca
     7561 ttttcaatca gatttttgat aattggataa aggatatctg ttttagtagt aaatggttcg
     7621 ttctttccaa tcaatcgaat cgtcaaccgt ttcttttcga tgattggctg ataattttga
     7681 acgatttgct ggaaataagg ttctgtataa attgtctgga tctcgtaaga attctcgcca
     7741 tcttttgaca gctggatgat ttcccgaatc agtttatcta accggatcgc atctttttgc
     7801 atgatctcaa gaaaagaagt cagagtttgc gggtcatctt ttgcaccatc cagtaaagtc
     7861 tcggtaaaac caatcagtga agtcacagga gtttttaatt cgtgagagac gttgccaacg
     7921 aaatcctttt gtagcttttc taaacgtcgc actttcgtta agtcatacgc cacccctaga
     7981 atctgtccgg taccttctga attagtaaag tagcgcaaac tcaagtctaa tgtctgccga
     8041 ccttctgtaa gggtgatctc ctgatggatc agcggatgct caggagtcac ttgatgaatc
     8101 agctgaatga gttttggctc ttgaatgact tctgtgtacc gctgttcagg ttgatagctt
     8161 accacgttta aatgtagctg catctttgga ttaagcagta gtaaacgaga atcagcatca
     8221 tcaatcagga atacgccaat catcagttcg ttcagtaacg tatataattg ctcttctgtg
     8281 gtagtacacg cccgatacat cttactcatt tgttcgctca acgcattaat cgtctgatac
     8341 aattcttccc attgatcggg tgtttgcatg attacttccg cctgattagg attttgcacc
     8401 atttttttta gtacgggcag cacggttttc aatggctggt ttttttgata gatcagataa
     8461 tagatcattc ctgcaagaac aaggaagaaa gacagaaaga agaaaaagac ccaacggcga
     8521 aaactctcag ttctcggtaa aaaaccgctt gtcggttctg cgatacgtaa aatggcttct
     8581 aactggccac tttttttgac tggtaatgcg acatataaaa gatcctcatt caaagtagta
     8641 cttttgcgca aagcgctacc taggttccca ccattcaata cggctttgat ttcgggacgg
     8701 gagtttcgct gttcattcaa tgtttcattg tttgtgtcaa ataagatctt tccagtagca
     8761 tccattaaag taatccgttc gttcgactga tggacgaatt cttccaaagc agttttgttt
     8821 tgcgctgctt ggaggttttc gacatctaat tgccggatca gcagttgtgc tttttttgtc
     8881 aggtagcttt cttgctgttc cacgatctgc tgttgaaaat aatgggaaat caattgccag
     8941 ccaccaaaaa acaaactaac taaaagaata agaaagaaga gacgttcata aatttttttc
     9001 catttcatcg ttttggttcc tgaaacttat agccaaatcc tctaatagtc accaagtact
     9061 ttggatgttt tggatctcgc tcgatttttt ctcgcaaatg actaacgtgt acatctacga
     9121 ttcggctttg tcctgcgaag tcaaaattcc aaatgcggtc tagcaatgtg tctcggtcga
     9181 tcacacggtc ttttcgtttc atgaaataaa caagtaattc aaattctttc ggtgttagtt
     9241 caatcagatg gtcgtctacg gtcacttgat aattggtcaa atcagcagtg atttgtccaa
     9301 tcgatagata ttcaggctca gcttcatctt ttgattgctt acgaggttca atccgtctga
     9361 atattgcttt catacgggct aatacttcgc gaggactaaa tggtttggtc aaatagtcgt
     9421 cagcacctat ttctaggcca atgatccgat ccacttggtc atctttagcc gtcagcatca
     9481 aaatcggtgt ttcatttttt tcttgacgca gccgctgagt gatctccatg ccatccatcg
     9541 ttggaagcat cacatctaaa atgatgaaat caaatgattg ttctaatgct aattctaatc
     9601 cttcgcctcc atctgtggca ctggttacct tgtagccttc tttttccaaa ttgaacgtca
     9661 agagcgttac aatagaaggt tcatcatcaa caactagtat ttttttcatt caaatctcct
     9721 ttaaataggt ttcattctgt ttatatttta gcatagaatc agtacaaagg gctattttac
     9781 aaaagaaata aatggtataa taggaaggca ctttgctgaa tagaaaggaa gagcagtcaa
     9841 tgattggaat cagtgcatgt ttaggcggtc tggcctgtcg ctatgatgga cagaaaaaag
     9901 aagtaccaga attgatggaa ctagttgaaa ctggagaagc tgtcatggtc tgtccagaag
     9961 tgatcggcgg attaccaatt cctagagatc ccgctgaaat cattggtgga aatggattcg
    10021 atgtgtggaa agatcaagca aaagtatgga cgacaagcgg cgaagatgta acggaagcgt
    10081 acaaaaatgg cgcgatcaaa gcctatcaaa aattgcagga aaaacacatt gatctagtca
    10141 tcatgaagga aaaaagccct tcatgcggaa cccattcgat ttatgacgga agtttttccg
    10201 gaataaaaaa aaatggtgcc ggagtggcta ctgcttattt tattcagcaa aaaatgactg
    10261 tgctctctga tgaagaatgg ctgaaaacta gaggtgagca gaatggaaat cgaaaagacc
    10321 aatcgaatga acgcgctctt tgaattttat tcgaccttat tgacagaaaa acaaatgaac
    10381 tacatggaat tatattatgc agatgatttt tcattaggtg agatcgcgga agaatacgaa
    10441 gtaagccgac aagcagtata tgacaatatc aagcggacaa gcaagatctt ggaggattat
    10501 gaaaaaaaac tccatctttt ttctaattat attgttcgag aacaggtatt ggaaaatatg
    10561 actgcatata ttacagataa gtacccggaa gataaaaaac tacttgaata tgtacagcaa
    10621 attcaagcaa tcgaagaata aataaaagga atgaagaaaa tatggcattt gaaagcttaa
    10681 ctgaacgtct ccagcaggcg atgggaaaaa taagaaaaaa aggaaaagta tcagaagctg
    10741 acgtaaaaga aatgatgcgg gaaatccgcc ttgccttact agaagccgat gttaacttgc
    10801 aagtagtcaa agattttact aagcgcgtac gcgaacgtgc ggtaggggca gaagtgctgg
    10861 aaagtttatc acctgcacaa caaatcgtaa aaatcgttga tgaagaattg acgatcacac
    10921 ttggttctga aacagcagag ttaaataaat caccaaaaat accaactgtt atcatgatgg
    10981 ctggtcttca aggggcaggt aaaacgacct ttactggtaa attagccaat tatttaaaga
    11041 aaaatgaaaa tgctcgtccg ctcctaatcg caggggacgt atatcgacca gctgcgatcg
    11101 accaattgaa agtattaggt caacagctag acgttcctgt atttgatatg ggaactgaag
    11161 tcagcccggt agagatcgtc cgtcaaggga tggagttagc aaaagaaaag aaaaacgact
    11221 atgtattgat cgatacggca ggacgtctgc atatcgatga aacattgatg gatgagttga
    11281 aacaaatcaa agaattgacg caaccaaatg agattttgct tgttgtcgat gccatgactg
    11341 gtcaagatgc cgtcaacgta gctgatagct tcaaccagca attaggtatt acaggggtcg
    11401 tcatcactaa acttgatggg gatactcgag gcggggcagc actatcgatc cgctcagtga
    11461 caggcgcacc aatcaaattc atcggttctg gtgaaaaatt gactgactta gaagtattcc
    11521 acccagatcg tatggctagc cgtatccttg gtatggggga tatgctgaca ttgattgaaa
    11581 aagcgcagca agactacgac gagaaaaaag cggaagaact tgcacagaaa atgcgggaaa
    11641 attcattcga tttcaatgac tttattgaac agcttgatca agtgatgggt atgggcccac
    11701 tggaagatct aatcaaaatg atcccaggaa tgaatcaagt ccctggaatc gaaaacgtca
    11761 aggtagatcc aaaagatgtt gaacgcaaaa aagcgatggt ttattctatg acgccagcag
    11821 aaagagcaaa tcctgatctg ttgaacccaa gccgtcgtcg ccgaattgct gccggttctg
    11881 gaaacagtgt tgttgaagtg aatcgaatga tcaaacaatt caaggaatca agaaaaatga
    11941 tgcagcaaat gtccaaaggg gatatgaaca tcccaggtat ggaccaaatg tttggttcag
    12001 gcgtcaaagg aaaactaggc aaaatggcga tgaatcgaat gatcaagaaa aataagaaga
    12061 aaaagaaaaa acggaaataa gatgaaaaaa acagcttcgg tacagactga aaatgtctac
    12121 tgccggagct tttctatcag caatttttat tctttactaa tacaattact ctaaacgaag
    12181 aggtggcaca gatgagatgt ccgtggagtg aacaaacaga aagtatgcgg caatatcatg
    12241 atactgtctg gggtgttcct gaatatgatg accaaaagct gttccgaaaa ttgatgcttg
    12301 atattaatca agccggattg agctggcaga cgattttaaa taaaagtgaa gcattcgatg
    12361 aagcatatga tcgattcgat atcaaaaaag tcgcaaatta tggagaaaca aaaatcagtg
    12421 aactaatgga aaacaaagga attattcgta accgaagaaa aatcgaagca gccgtccaaa
    12481 acgccaaagc agtcttgaaa attcaagaag aaatcggcag tttttcagag tatctttggt
    12541 cgttttctaa tggaaaaatc gttgattctt cttatcattc acaagaagaa gtcccaacaa
    12601 caagtcctct ttcagatgaa ttagcaaaag atctaaagaa aagaggattt aaatttatcg
    12661 gaagcacaac aatctatgct tttttagaag ctgtcgggat catcaatgat caccttgaca
    12721 gttgtttccg caaagaagag gtcgtacgta tcggcagaaa gggcgaataa agcatgcgaa
    12781 aaatcatatt ttatggtgct ataagtttag atggctattt ggcaacgaaa acggatgatc
    12841 ttcaatggct ttttgacact ccaacaggta agcaaactac ttatgaagct ttttatgaaa
    12901 caatcgatac gaccatcatg ggcagaaaaa cttatcagga agcaaaaaaa tatttggaga
    12961 ccgaagcaat ttatccagag aaaaagaatt tcgttttttc cacccacgca caattacaat
    13021 tgccagatgc tgaggtcgta aacgaagatc ctgttgcttt tctaaaaaat ctacaaatga
    13081 cagaaggaaa agatatctgg gttgtgggtg gcggtcgatt gatcaaaccg ctattggaac
    13141 atcaaatgat tgatgaatgg tatatccaga ttacccctgt attattaggg gacgggattc
    13201 ctttattcca aaaaggaaca tatgaagcgc gttttgaaat gctagacacg acacgattcg
    13261 gtgaatttat cgaattgcat tatgtacgaa agtagaagta aataaaataa aaaaggcaac
    13321 tatatgcaga aatgtcctgt acatagttgc cttttttatt accgttatgc tttttactct
    13381 gttaatttgt tagaaaaatc tagatacgca tctaaaatcg tttggaagaa acttaatgtt
    13441 ccttcaacga tattaccatt ttcatcaatt agattcgcaa cgtttccgat atatgcttcc
    13501 ggttgctgca aggttggcac attcaaaaag acaagtgatt ggcgtagatg gtggtttgcg
    13561 ccaaagccgc cgatcccacc tggtgatgca gtaacgacta aacctggttt atgatcccaa
    13621 acactgtgtc cataaggtct tgagcctaca tctaaagcat ttttcaatac tgctggcaca
    13681 gaacggttgt attcaggagt aacgaaaacg acaccgtcta gtcccctcac ttcctcacga
    13741 aaacgagtcc attcagcagg agcctgttct ggagtttcga gatcctcatt gtaaaatggc
    13801 agatcatcga tttttacaaa tacactttcg tacccttcag gaagcaattt cgcaaaagct
    13861 tctgctactt ttttgttgta agaatctttt cttaaactac caataaaaaa tccaattttt
    13921 tttgtcatac gatttttcct ccttcaaact atctataata atcttacaaa agataagtaa
    13981 ctggagcaaa taaaaacact tactgacaaa agctacatag aagatagatt acttattttt
    14041 aacaaaaaag aagtctaaaa tcttctattt tctaaaaaaa atcgaattta atcaagctgt
    14101 aaagaaaaaa agctttacag ggtaaaaaaa aactgttaca ctacatcttg tgaataaaac
    14161 aatggaggtg gaaataaata atggcagtta aaatccgttt aaaacgtatg ggttctaaaa
    14221 agagtccgtt ttaccgtatc gtcgtagctg attcacgttc tcctcgtgat ggacgtttca
    14281 tcgaaactgt aggtacttac aatcctttga aagaccctgc agaagtagtt ttaaaagaag
    14341 acttagttct tgactggtta tcaaaaggtg cacaaccttc tgacactgta cgtaacatcc
    14401 tttcaaaaga aggcgttatg caaaaacacc acgaagctaa attctcaaag aaataaggtg
    14461 accggatatg aaagatttaa gcgacttagt cttaacaatc gttcgtccgc tagtcactta
    14521 tcctgatcaa gtcaagttag acgtcgtaga gtcgaaagac ttttacgagt acaatctaac
    14581 agtggctcct gaagatattg gccgtatcat tggaaaacaa gggcgtgttg ctaaagcaat
    14641 ccgcacaatc gtttacggtg ttcggatcaa tgcaccgaaa aaagtgcgtt tgaatatcat
    14701 cgatgataag gaaaagtaaa taaacgtgct gtactcgaat tttcgagtgc agcttttttg
    14761 ttttttttat taattgaaga aatgattatt tggcaaaaca agcaagaaag cgtatcctta
    14821 taggaggagg gagtgaacag ccaatgagaa agaaaatgtt atttattcca tttgctctat
    14881 tgttaagcgc ttgtagttcg gttactcaag aatcagacaa tacacatcta tcaagcagta
    14941 tatctgaaac tgttaccagt ccagaaaaaa gtagtaccaa aacggcttcc accactgaac
    15001 aaacaatggc tacctcaaat aatgagaaaa agactgcttt agaccagttg aaagaacaac
    15061 agccaaatgt tcccatgcct ctagatgtac cagtctcttc aggctatctc aatatcgcag
    15121 cgacacatac aaagcaaggt tattctattt tatattacag aacagatcgt cccctcgggt
    15181 tgaatgctga cgaattgaat caagaaacgc ctattgcaac ctatctatat caatatgggt
    15241 ttgcttctag tcaagaaaca atccaagttt tgcagccatt tgagatcgac acaaatggtc
    15301 agcaagtgga cttaggttct cgaatcacag ggtatcagca aggtgcagca ggttcaagtt
    15361 ttcttgaatg gcaagaagga aactggcgta ttcgaatccg tgggaataat atagaaggcc
    15421 aagatccctt actattagct aaagaaatcg tagcttattt ggaagaaaac agtctaccag
    15481 cacctgaaca atttggtaaa attactgttg atatgggtga tacgacgaat cgagccgtag
    15541 aagtaagctg gcaagaaccg aaaaatgcct atacgatcac tcatcaagat cctatgtctg
    15601 cgttgaagat ggcagtttct atgaagaggt tgtgaaatca gcgcgctgat ctgagcccat
    15661 catttaactc caagtcacag gaagtaatca aaaatagcac gccgcatttt tgcataattt
    15721 tggcttaata ccttaaaaga aaactagaat tgcctcttca cacatgctaa aataaaacag
    15781 acaaaaaaga aatggggata taagttgact gaatacttga atgtaggaaa aatcgtaaac
    15841 acacaaggta tcaaaggcga agtccgtgtt atctcaacaa cggatttccc agaagaacgt
    15901 tacaaaaaag gaacggtgct aacactattc caagacggaa aagcacctgt ggaattaaca
    15961 gtcaaaagtc atcgcaaaca taaaaatttt gacttattga gttttgaagg acatccttct
    16021 atcaatgatg tggaaaaata tcgggatggc atattaaaag tgtcaaaaga caactctgta
    16081 gatctagctg aaaatgaatt ctattaccat gagatcatcg gtctgcatgt attagaagat
    16141 ggaactgagt taggtaaagt aaaagaaatc ttgtcaccag gtgccaatga tgtttgggtc
    16201 gttcaacgtc cgaaaaaagc tgatatattg cttccatata tcgcttctgt cgtcaaagaa
    16261 gtcgatttag aagctggagt ggtccgtgta gaaattccag aaggactgat tgacgatgaa
    16321 gattgatgta ttgactttat ttccaagaat gtttgaaggt cctatggggg aatccatcat
    16381 cggtaaggct gtagataaag gattattaga tatcaatatc tctaacttcc gcgattattc
    16441 agataacaaa catcagacag tagatgatta tccatacggc ggtggagcag gtatgctgct
    16501 caaagtccag cccatctatg ataacatcca agccattgaa aaagctgcac cggatgtcaa
    16561 aaaaagagta attcttcttg acccagctgg taaacgattc gatcagaaaa tggcagaaga
    16621 attttcacaa gaagaacact tagtattcat ttgtggtcat tatgagggat atgatgaacg
    16681 tatccgcaca ttagtgacag atgaagtttc cttaggagat tatgtcctta ctgggggaga
    16741 attaggtgcg atggtcatga tcgatgcaac agttcggctt ttgccagatg tgttagggaa
    16801 tcagacttca gctcaaacgg attcttattc aaccggttta ttggaacatc cccaatatac
    16861 tcgcccggca gaatataaag ggatgaaagt accagaagta ttgacaaatg gaaatcataa
    16921 attgattgaa gaatggcaat taaaagaatc actaagaagg acctatcaac gtcggcctga
    16981 tcttttggaa acacttgaat ggacacctca aatgacgaaa tttttagaag aaatcaagaa
    17041 agaagaacaa gaaaaaacag cagatgaagc caagtgactt tttctaaaat cttctttaca
    17101 gtaggttgat aatatgttag tattttatgg tgagtgcaaa agcacttaac tattacgata
    17161 ttccgctgtg agaaaagctc ataagaatat ttggaataag gagaagaaaa ccatgaatcc
    17221 attaatcgaa gaattaacaa aagaacaatt acgttctgac atcccagcgt tccgccctgg
    17281 tgacactgtg cgtgttcacg caaaagttgt tgaaggtact cgtgaacgta tccagttatt
    17341 tgaaggtgtt gttatcaaac gccgcggtgc tggaatcagc gaaacttata cagtacgtaa
    17401 agtttctaac ggagtgggcg ttgaacgtac attcccattg catacaccac gtgttgcaaa
    17461 aatcgaagta gttcgctacg gtaaagtacg tcgtgcaaaa ttgtactacc tacgtgcatt
    17521 acacggaaaa gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga
    17581 aatctgaaat ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca
    17641 gttttaagcc atttttggct gttttggacc atttttcaat catattccct taaaaattgt
    17701 cctagattgt ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag
    17761 cttgacgaca aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga
    17821 acccaagttt ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact
    17881 aaaaagttga tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca
    17941 aaatgatgga cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa
    18001 tgaattcatc gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga
    18061 aaatccaaat tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa
    18121 tttattgatt agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga
    18181 acaagaatta ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg
    18241 aatgtacgaa caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat
    18301 gttaggagat atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt
    18361 cgaactgctg ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc
    18421 aatgacaaag tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat
    18481 gaaaagaacg aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag
    18541 aatcaaaatt tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca
    18601 ctctaaatca gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa
    18661 ggagatttat tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag
    18721 tctattatgc caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat
    18781 tactcatcca caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg
    18841 tacatttagc gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa
    18901 tggtgtgaag caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac
    18961 tcctggttat gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt
    19021 tttatggaag gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg
    19081 gcaagaagtt aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa
    19141 tatcgcagcc attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag
    19201 cttccataac tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa
    19261 cggcttgaat ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg
    19321 tgtgaacggc aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt
    19381 aaccctaaag aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca
    19441 aactttgatg gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga
    19501 aactgagggt tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg
    19561 aaccgttttc catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa
    19621 agaaggcatt gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa
    19681 atcagtgcca gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg
    19741 tgagactatt tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa
    19801 aatgggggtg gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac
    19861 gtttgccatt cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga
    19921 aaaaggtcat gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta
    19981 tgtgaccgaa accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga
    20041 attgaactat gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca
    20101 agaaatcact ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca
    20161 aggtaaagct gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc
    20221 ggtcaaatgg agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga
    20281 aacggtgact ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa
    20341 tgagtattac tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta
    20401 ccctgtttcc atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt
    20461 tacggcaaaa gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc
    20521 tgctgggact gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg
    20581 aaccagtgag attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg
    20641 ttttgatggc tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt
    20701 agaggctcca gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga
    20761 aaataaagcg gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa
    20821 acaaccaatt aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt
    20881 tagcttgaat cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct
    20941 tgggacttgg aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga
    21001 taaattgagc tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat
    21061 tgatgtggaa gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat
    21121 tgccgaaaca accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca
    21181 taaattaacc gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga
    21241 aaacaagaaa ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa
    21301 tcaagcccaa actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca
    21361 cctagaagat ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt
    21421 gtcgattacc catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc
    21481 attacttccg gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt
    21541 gaatgacaaa gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc
    21601 agaaggtaca agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg
    21661 gaaacacaat gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac
    21721 aacaccaagt gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc
    21781 tagtcctaca gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt
    21841 tattgggttt accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta
    21901 aggtgatgtg atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa
    21961 aagtggtcgc gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa
    22021 ttgaaatttg ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa
    22081 ggcgaagttt taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat
    22141 ttgtattcca gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc
    22201 gaaaaggatt ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa
    22261 ggctatgaga ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg
    22321 aaagcatagg aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta
    22381 acgtttggga aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat
    22441 ggaacggtta gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt
    22501 cgaatgatcc aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca
    22561 gaggtagagt tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga
    22621 actactgtca attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag
    22681 ggaaattcta caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt
    22741 gaacatattt tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc
    22801 actaaagcat taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa
    22861 gtaggtaacg atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc
    22921 ttctttatgg ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa
    22981 gaaattggtg tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg
    23041 aaacaactcc ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat
    23101 cctatgaagc ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat
    23161 acgggcagga gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt
    23221 catcggttgg atgagcgtct tttattttca agagcttaga caatttgata tttctaagct
    23281 atcactttct actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag
    23341 tctcgttctt tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg
    23401 cagtttgtgg catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt
    23461 aaaacaaact cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac
    23521 aatcagttac ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt
    23581 tcaaatttca ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag
    23641 tggtctatat tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt
    23701 actgtatgat ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc
    23761 tttgcgattg atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc
    23821 tggcggcaca ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa
    23881 atctgatgcc gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac
    23941 ggtaatgcct catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta
    24001 tgagcgcatg atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga
    24061 gaattatgcg tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta
    24121 tatggaaatg ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt
    24181 gatgttaggt cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa
    24241 atatttagga gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag
    24301 tgaactggga tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat
    24361 caaaggtcga ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact
    24421 tgtaccaaaa ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa
    24481 tacggaagga taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt
    24541 acgaggtgat ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt
    24601 atggaacaag cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag
    24661 agttataagg caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta
    24721 tcagacgagt ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc
    24781 caagcttttt caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc
    24841 tttgaaatcg ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc
    24901 cggggattac tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat
    24961 ggattggggt atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac
    25021 cgtgagcgga tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct
    25081 atcattggat cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct
    25141 tccaatcgag gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc
    25201 ggtgatgaag gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg
    25261 ttactgggaa attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact
    25321 ttgtcaacga aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa
    25381 gaggtgaagc cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc
    25441 cgtctgggaa aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg
    25501 gatcagtatc aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg
    25561 gactgggctt ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct
    25621 ggaaattctg taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa
    25681 acagatgatt tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca
    25741 gatccggaat tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg
    25801 aaagtccctg atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat
    25861 caagtcatca gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa
    25921 tacttgtttg agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca
    25981 aataaaccac tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg
    26041 ctgtgcctgt ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag
    26101 gggggtacaa aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg
    26161 ataataagca atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga
    26221 ggtgagaact tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa
    26281 tatggcgttt cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat
    26341 cagatagaaa atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa
    26401 ctggaaagtt tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc
    26461 tttcccacaa cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat
    26521 atgctccacg aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt
    26581 gtggtgatgt tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt
    26641 tgtcggcagt ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa
    26701 gattgtctaa aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt
    26761 ggcctgttag atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg
    26821 tttcgtacct tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt
    26881 atgggcaata ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa
    26941 aaagactatg agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat
    27001 cgttttgaaa tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa
    27061 ggtcgcagta ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat
    27121 aaggtggaag gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt
    27181 ggacggaatc agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga
    27241 acgttgaact ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat
    27301 cggttgaatc agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg
    27361 cacaaaaaga ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag
    27421 gaatcgtgat ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg
    27481 ttactgtgat gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag
    27541 gaaagtgatg aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa
    27601 tctttagtct tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt
    27661 tccaaactga ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct
    27721 tctggtgtga aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga
    27781 atcacaatgg aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag
    27841 ttggtggggg catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga
    27901 ttaaatgacg aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat
    27961 gagtatacac caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga
    28021 acgccaattg gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa
    28081 atggtagagc atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta
    28141 gcgtattact tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg
    28201 tatattgatt ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg
    28261 agtcgagggg tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc
    28321 gtacttttac ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta
    28381 ctaatggttg gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag
    28441 tgctttcagg ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga
    28501 ctcgattgtg ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa
    28561 gtggctatcg aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat
    28621 ggaactcatt tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc
    28681 ttaaccaaga tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa
    28741 tcagttttag aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat
    28801 atagatgagt ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa
    28861 tcagaaaata tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa
    28921 atgattccct catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt
    28981 agagttggaa aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat
    29041 aatatgacat ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga
    29101 ctttggggaa taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga
    29161 tcacttcgcg atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc
    29221 tccattaatg aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc
    29281 acaaaagaag ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga
    29341 acaggcaaat cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt
    29401 gatcaatttt ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc
    29461 tctggaatat ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa
    29521 tatcaaataa taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt
    29581 ctggttgaaa aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct
    29641 caaaaacaaa tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga
    29701 tataactctg atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata
    29761 agaaaattaa attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta
    29821 gagcttgaaa ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa
    29881 gatgtttatg atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg
    29941 gaggcactta aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc
    30001 aatggtataa aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta
    30061 gtgaccaatc gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt
    30121 tatagagcag agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg
    30181 tatcaaagag gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac
    30241 aaaaccttga ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta
    30301 ttcaacaaga aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa
    30361 tgaataaatc atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt
    30421 ctgatattac aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta
    30481 aagagctcag agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta
    30541 ttgataagct gacaaaaaat tccattacta atcaagatat atataatgac atacttgata
    30601 taagagttag tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa
    30661 aattgaatga tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta
    30721 tccgacttga actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga
    30781 catctgcaat tctttctatg attttggcgt atggaaattc accactcata ttggatcagc
    30841 ctgaagatga tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa
    30901 aaaaggaaaa cagacaaatt ttaattgtaa ctcataatgc aaatatccca gttaatggtg
    30961 attcagaatg gttgattagc atgggagatt caaaagaaat atcggctgat cagtgcgggt
    31021 cggtagatga acccaatatc aagccaagaa tatgtactgt gatggaaggt ggcgaggatg
    31081 cttttacaaa tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa cactagaggg
    31141 tgagacatat tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat
    31201 accagtatct gggcagttga aaaagtcatt tacgcaatca atgattttca gttgcctttt
    31261 cctgtgactt ttaaccaaat ggcttggttc gttttatctt tactgtttgt catcgtattt
    31321 gcccatgtgc ctccactatc tatgatagag gaggtctttt taaaatactt aggcataccg
    31381 gtggctgtca cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc actgggattt
    31441 ttacgatcct ttatcagcta tcacttgcga tttaaggtga cttttgcagg aaagaaagtc
    31501 aaagaacaga agaaacgatg ggatgaaccc agtactcttg taaggagtgt gaactatgta
    31561 tccgattaag tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt gttttgccta
    31621 ctatgaatta gtgccttaca attactcctt tttgtcaccc gaacagaaat ttcaagtgca
    31681 tgataacttt cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat
    31741 tgccacagaa agtagtattc gcgccacaca agaacgatca aaaaaagaaa ttaccggtag
    31801 actcaaagaa gtagcgaaac aacgaattga tatacagaca gatgcattag tatccatgat
    31861 tggcgatagt cagattgatt atcgcttttt tattggcttt aaactaattg cgacagatga
    31921 agaagtcaat ctaaagagtc tcaagaaatc tttcttttct gggcttcaag aatttgtcta
    31981 tggagtgaat catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgctg
    32041 ctattctaag cttgaaaaat taatggaaag caaattggct cgaagattta aagtcaggcg
    32101 agtcacaccg agtgacctga cgtatttgat tgaacatatt tatggcgaaa aagggatacc
    32161 gtttgaagaa tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa
    32221 acgttatgac ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg
    32281 gatggaacat gagaatcatg agtcgtacgt agcgtattta acaattaata caattgtggg
    32341 ggagatggaa tttccttcct cagaactttt ctattaccag caacaacaat ttacattccc
    32401 aatcgacaca agtatgaatg tagagattgt gacgaataaa aaggctttag ctaccgttcg
    32461 taataagaaa aaagaactga aagatttaga caatcatgct taccaatctg acaatgaaac
    32521 gaattccaat gtcttagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca
    32581 gtcaaaagaa tctatgtaca aactgagtta tgtggttcga gtgagtgccg aatcagtgga
    32641 tgaactaaag cgacgctgtg atgaagtgct cgatttttat gatgatacca atgtaaaact
    32701 agtccggccc tttggcgata tgatggggct acatgaagaa ttcttaccct taagtaaacg
    32761 atatatgaac gactatattc agtatgttac gtctgatttt ttggcggggc ttggttttgg
    32821 ggctactcag atgctaggag aattagaagg gatttacttt ggctacaatg tggatacagg
    32881 tcgtaatgtt tacctgaaac cggcattggc ttcacaagga gtcaagggtt cagtaaccaa
    32941 tgcgctggcc gccgctttcc tgggttctct tggtggaggt aaatccttta gtaacaatct
    33001 cttagtctac tatgctgttt tgttcggtgg tcaagctgtg attgtcgatc caaaaggggg
    33061 tgcgaaacgt tcctacttga aaagagtagg cacagcactt cattaaatca atatttaatg
    33121 tgtgttggag aactaaacac acgaatagtc gaatgacggg gtaacgccct gaaaggctac
    33181 tttaatactc tgactggcag aaacttgagt aaacaaggct ctgtcagaag ctcggtgaag
    33241 tcggcagaca gacaccgtaa cagataatgc tgacgaaaga ttcccagagg tgggcgttgt
    33301 gtcctatatg ccggaagtct ataaaatatc tatggttaga atgtagatga gaggtctact
    33361 gacgaatcct tgaatgtaca ggtctatatc ggggctggat agaaatgtcc agtgacacaa
    33421 acagtgtcgc tactgtgggt gagtaagact ccgcatttat gaaaatccat ataacgttaa
    33481 aggcgttatc aaggtagcag gcttatagag gaaacctaag gatatatgta cagatagtgt
    33541 gtttggaacg ttgaaagctg acaacatgga gattttactg tctgtgaagt gttcacatca
    33601 gaaagttatt gtgagattag caatgatata aggtgtgtta atgtcagtga gagtggtggc
    33661 acagtaccta tgaaaccgtg ataacaagcg gtggagggat agccactagt catatgaaga
    33721 atagaattac taagaaaaat ggtatgggtt cgagtaagac taagagatgt acttgctcct
    33781 gcaaacaagg agggtacaga ccatgctaaa gaaaaacaag ctccgctata acgagtatta
    33841 tgatatgcaa catatttatg acgagttata cgcacagagc aagaacggta ataactttta
    33901 taagttactt gaaattattg gttcggaaca aaatatttgt ttagcttacc gtaatctgaa
    33961 atcaaacagt ggcagtaaaa cggctggaac ggatggaatg acgatagacg atataaaaca
    34021 actcagcaat gctgagattg ttgctaccgt tcgggaaagc cttagcaact accgccccaa
    34081 atcagtcaga cgtgttttca ttccaaaagc tgggagcgat aaaatgcgtc cattggggat
    34141 tccgtgtatc tgggacaggc tggtacaaca atgtatcctg caagtcctag agccgatatg
    34201 cgaaccaaag ttccataatc attcctatgg attccgtgcg aatcgaagtg cccaccatgc
    34261 agtcagcaga gtaacgacac tgataaattt atcaaaatat cattactgtg ttgacgtaga
    34321 cattaaagga tttttcgata atgtcaatca tggaaaacta ttaaaacaaa tctggacact
    34381 tggtattcgg gataaaagat tgatttgcat tatcagcaaa atgctgaaag ccgaaattga
    34441 cggcgagggt gtcccagaaa aaggaacgcc acaaggcggt ctgttaagtc cactgctctc
    34501 cctcatagtc cttaatgaat tagactggtg ggtaagcagt cagtgggaaa cattccagcc
    34561 gaagaaccgc agtaaaaatg ggtggcttca atacgcaaag aaatatacca aactgaaaag
    34621 cggttttatt gtacgttacg ctgatgattt taaaatcatg tgttcaacct atggagaagc
    34681 acaaagattt taccacagta ccgtagattt tcttaataaa aggctgaaat tagaaatcag
    34741 tccagagaaa tcaaaggtag taaatctgaa gaaaaattca tcagattttc tggggttcaa
    34801 aatcaaagtc attccaaaag gaagaacgaa acacggttat gttgccaaaa cagatatgaa
    34861 ccaaaaagca ttaaagaaag cgaaaacaaa tctaaagtta aaggtaaaag atatagccag
    34921 acatacgact ggcttcaata tcagcagata taatctgaca gttatcggta tgcagaatta
    34981 ctactgcata gcaactaacg tatataacaa cctgacagaa gtaagttacg ctctcttacc
    35041 gaccatacga atccgtttaa ggaacattgc aaaatcagtt ccatttgaaa gtacaagttc
    35101 agaatttcag agcaggacaa aaggaatcag accaaaaaca aaaatagtga tgattgcgga
    35161 taatccgcta ttgccgattc aaggagtaca acacaaaaat cctatgaatt tctcgcaaga
    35221 tatttgtaat tttacaaagc aagggagaaa caaggtgcat gaggacgttg tagttgtaac
    35281 aaaagaagaa atccgagcgt tgttggaaaa tgaaaaccct gccgatagcg tagagtttaa
    35341 cgacaatcgt atttcggcat atatcgcaca acaaggcaac tgctatgtaa tgaatcgtcg
    35401 aggaactccg tccactctga tatgtattca caaatcagac aaaggagata accttgacag
    35461 atattctaat ctggcatttg tggaaattcc cattgcaaag gcaatcctaa cagaatcagc
    35521 agacgaagca aaatcactgt taaaaggtta tgttcttaat agtcagcaga agaaaaaact
    35581 gaacagaata cggacgaatt acggatacca gacaataact gggaaataac ttataaatct
    35641 atttggatat gatggaacgc cgtatgaagc gaaagtttca tgtacggtgt gaagcggggg
    35701 aaaacttgga gataacatca aaaagttacc tatcgctata gcgaggaggg tggaaagaaa
    35761 ccttgccaga aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc
    35821 aaggtctgct tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa
    35881 ttgatattct gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt
    35941 tacgtaaagc cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta
    36001 acgaattgcg aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct
    36061 ttaccgatta tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta
    36121 gtttagataa acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa
    36181 cgacttttga agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt
    36241 ccacttttgc cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg
    36301 atgaggcttg gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc
    36361 gtgctggtcg ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat
    36421 tagatgaacg tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc
    36481 atgaaatcaa gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa
    36541 aacgcctgcg agatttggaa aacg
//
LOCUS       DACCCS010000024        36240 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.024, whole genome shotgun sequence.
ACCESSION   DACCCS010000024 DACCCS010000000
VERSION     DACCCS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.024"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..1235)
                     /locus_tag="IVK82_001243"
     CDS             complement(<1..1235)
                     /locus_tag="IVK82_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP6392316.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQK"
     gene            complement(1228..2820)
                     /locus_tag="IVK82_001244"
     CDS             complement(1228..2820)
                     /locus_tag="IVK82_001244"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP6392317.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(3330..4055)
                     /locus_tag="IVK82_001245"
     CDS             complement(3330..4055)
                     /locus_tag="IVK82_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392318.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(4209..4535)
                     /locus_tag="IVK82_001246"
     CDS             complement(4209..4535)
                     /locus_tag="IVK82_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392319.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(4572..4937)
                     /locus_tag="IVK82_001247"
     CDS             complement(4572..4937)
                     /locus_tag="IVK82_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP6392320.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(4974..5189)
                     /locus_tag="IVK82_001248"
     CDS             complement(4974..5189)
                     /locus_tag="IVK82_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6392321.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            5318..5653
                     /locus_tag="IVK82_001249"
     CDS             5318..5653
                     /locus_tag="IVK82_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6392322.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(5729..7726)
                     /locus_tag="IVK82_001250"
     CDS             complement(5729..7726)
                     /locus_tag="IVK82_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392323.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(7824..8099)
                     /locus_tag="IVK82_001251"
     CDS             complement(7824..8099)
                     /locus_tag="IVK82_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6392324.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            8331..9065
                     /locus_tag="IVK82_001252"
     CDS             8331..9065
                     /locus_tag="IVK82_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6392325.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            9104..10246
                     /locus_tag="IVK82_001253"
     CDS             9104..10246
                     /locus_tag="IVK82_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6392326.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(10874..11359)
                     /locus_tag="IVK82_001254"
     CDS             complement(10874..11359)
                     /locus_tag="IVK82_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HAP6392327.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(11361..12797)
                     /locus_tag="IVK82_001255"
     CDS             complement(11361..12797)
                     /locus_tag="IVK82_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392328.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPH
                     YCIMRKILKENFVDSEDFFDQERYNYELTKRRLQINDLSRFSINVVEVDDYSEITDIL
                     KSIRKKYLRKTIFISGSAVDYTPFTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGN
                     SIVSGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLE
                     SGEIVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQ
                     ALEKLDTEGEVDKKVEQIVSLINSIQED"
     gene            complement(12830..13681)
                     /locus_tag="IVK82_001256"
     CDS             complement(12830..13681)
                     /locus_tag="IVK82_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392329.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            complement(13837..14082)
                     /locus_tag="IVK82_001257"
     CDS             complement(13837..14082)
                     /locus_tag="IVK82_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6392330.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            14187..14960
                     /locus_tag="IVK82_001258"
     CDS             14187..14960
                     /locus_tag="IVK82_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392331.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLNVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            complement(15013..15327)
                     /locus_tag="IVK82_001259"
     CDS             complement(15013..15327)
                     /locus_tag="IVK82_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392332.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(16846..17064)
                     /locus_tag="IVK82_001260"
     CDS             complement(16846..17064)
                     /locus_tag="IVK82_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392333.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            complement(17079..19706)
                     /locus_tag="IVK82_001261"
     CDS             complement(17079..19706)
                     /locus_tag="IVK82_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392334.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            complement(19693..19911)
                     /locus_tag="IVK82_001262"
     CDS             complement(19693..19911)
                     /locus_tag="IVK82_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392335.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            complement(19908..20783)
                     /locus_tag="IVK82_001263"
     CDS             complement(19908..20783)
                     /locus_tag="IVK82_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392336.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            complement(21048..21347)
                     /locus_tag="IVK82_001264"
     CDS             complement(21048..21347)
                     /locus_tag="IVK82_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6392337.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            21549..22346
                     /locus_tag="IVK82_001265"
     CDS             21549..22346
                     /locus_tag="IVK82_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6392338.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
     gene            22397..23539
                     /locus_tag="IVK82_001266"
     CDS             22397..23539
                     /locus_tag="IVK82_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6392339.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(23637..25028)
                     /gene="sufB"
                     /locus_tag="IVK82_001267"
     CDS             complement(23637..25028)
                     /gene="sufB"
                     /locus_tag="IVK82_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP6392340.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(25033..25503)
                     /locus_tag="IVK82_001268"
     CDS             complement(25033..25503)
                     /locus_tag="IVK82_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP6392341.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(25490..26725)
                     /locus_tag="IVK82_001269"
     CDS             complement(25490..26725)
                     /locus_tag="IVK82_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP6392342.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(26722..28008)
                     /gene="sufD"
                     /locus_tag="IVK82_001270"
     CDS             complement(26722..28008)
                     /gene="sufD"
                     /locus_tag="IVK82_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP6392343.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(28021..28794)
                     /gene="sufC"
                     /locus_tag="IVK82_001271"
     CDS             complement(28021..28794)
                     /gene="sufC"
                     /locus_tag="IVK82_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP6392344.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(28991..29839)
                     /locus_tag="IVK82_001272"
     CDS             complement(28991..29839)
                     /locus_tag="IVK82_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6392345.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(29863..30549)
                     /locus_tag="IVK82_001273"
     CDS             complement(29863..30549)
                     /locus_tag="IVK82_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6392346.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(30542..31576)
                     /locus_tag="IVK82_001274"
     CDS             complement(30542..31576)
                     /locus_tag="IVK82_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6392347.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(31971..32309)
                     /locus_tag="IVK82_001275"
     CDS             complement(31971..32309)
                     /locus_tag="IVK82_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP6392348.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(32311..32667)
                     /locus_tag="IVK82_001276"
     CDS             complement(32311..32667)
                     /locus_tag="IVK82_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP6392349.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(32673..33860)
                     /locus_tag="IVK82_001277"
     CDS             complement(32673..33860)
                     /locus_tag="IVK82_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP6392350.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(34051..34896)
                     /locus_tag="IVK82_001278"
     CDS             complement(34051..34896)
                     /locus_tag="IVK82_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6392351.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            35130..36095
                     /locus_tag="IVK82_001279"
     CDS             35130..36095
                     /locus_tag="IVK82_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP6392352.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT        10880 a         7142 c         5616 g        12602 t
ORIGIN      
        1 atcttttgta agaagccttt tttcgtttcc ttcagcaaat ctaacttacg ttgatgaaga
       61 gcgatcgtgt cgtcgagttg tttgaagaaa gcgccgattt tttgttgttc ctctttattc
      121 ctagtaaaag gaaacttgat attagcgaaa ttctcataga ataagtattc tcgtacgctt
      181 ccttcagtgt tgcgcttgac ctctttgata aatgttggtg acttgataaa ctgaagtatg
      241 aactcattat ctaagtcctc gctcattttc acaactacat aaagagagct aacaatgacc
      301 gtcataccaa gattgttaaa tgcaattgat cctacgttta ctcttgctgg attataggcg
      361 aattcatttg gttcaacgaa tttataattt gttttttcta aatcatccaa cctactacca
      421 tcaaactgtt ctgtttgaga aattaaacct gctttattac ttaccgaata ggcagggaag
      481 tctaaatctt tcgtgttctt ttttccagtc ttcttactaa actctttgaa cttacgctgt
      541 tcccaatctt ccgtaaatcc aggaaaccga atttctggca cttttgctcc gtttttaggg
      601 aacatttttt gtaagaatcc ttttttcgtt tcctttaata aatctagctt acgttgttga
      661 agagcgatag tgtcgtccag ttgtttgaag aagttcccaa ttttcttttg ttcttctaat
      721 attggaaatt gtactatcca ttttttcact tctgacattg gtacgagatt tgtaattgct
      781 gaacctggat tagctcctgt gagttttctt tgcataaaat ctgcacgaat aaattgaata
      841 aaatattctg aatctgtgat ttctttattt attctaagtc taatcaaagc ctgattgaat
      901 acacccggtt ttatttgctt tggtactctt gaaattctcc caattgttcc agcgccactc
      961 atgataaaat cgcctggttc taatttaaat ttgattaatt cattatattt tttttcagta
     1021 attttatatc ttgtttcgta attatcataa atagcatttt gttgttcata gacaacaaaa
     1081 ttgctattag atacaaagaa ttctttcttt aaggcactcc caaatggtcc cctccttact
     1141 ccatcttttt tatcaagtaa gcattcaaac ttacgctgtt cccaagcttc cgtaaatcct
     1201 gggaaacgaa tttctggttg tatatcatta ctcatgtttg aacacctcca gcgcaccttg
     1261 taaccacgct tcattttcag gtgttgtttg cagggaagag atagcttcta aaagttcttt
     1321 ttctagcact tgctgttctt gtcgtatctt cttgatgtca tttccgacat gaaccatatc
     1381 aattggttct tcttcttcaa acgtatctac atagcgtgga atatttaagt tatagtcatt
     1441 ttctttgatt tcatcaaagt ttgccaaatg agcgtatttc tcgacatctt ttctttcagc
     1501 ataggtttcg aggatttttt gaatgttttc ttccgataat ttattctgat tttttccttt
     1561 gacaaattct cgactagcgt caatgaataa tacgtctcga gtttggcggt tctttttcaa
     1621 tacaatcacc gtcgttggaa tcgatgtccc gaagaacaag ttcgcaggca taccgatcac
     1681 agcataaata ctgccgtctt ctagtaattt ttgacgaatg accccttctg cagcgccacg
     1741 gaataaaact ccgtgtggca agacgatggc catagtccct gtttccttca aatgatagaa
     1801 accatgaaga agaaacgcaa aatctgcttt ggattttggc gctaattttc cataacgatt
     1861 aaaccgagaa tcgtctaaga aggtcgtatc tgctgaccaa ttagccgaat aaggtgggtt
     1921 cattactact gcatcaaatg tgtaaggttc atctgttggc caatccttat tcaacgtatc
     1981 cccattgcgg agattcattt cttcggcatc taccccatgt aaaatcaagt tcatttttgc
     2041 tagattgtat gtcgttgtat ttaattcttg cccatgatat ttaacgttat ctgggtgagt
     2101 taagtagtta cgcacattta acatcaatga acctgaaccc atggttggat caaaaacact
     2161 aaagaatcgt cgttcttttt gatccaacgt cacgatttgt gccatcatat cagaaaccat
     2221 atgaggcgtg taaaattctc cggctttctt ccctgcttct gaagcaaatt gactgatcag
     2281 aaattcataa gcatccccga tcacgtcccc atcgtgacct aaaacttcca cgtcattcaa
     2341 tttcttaatg acttcggtaa tcgtcacatt tcgttgttgc tcatctgttc ctagtttttt
     2401 tgattggaga tccacatcat caaataaccc attaaattga ttgtaggtag atgccaattg
     2461 cacaaaggct ttgtttagat cattcaattg gaacgttgct tgttttgctt gatccgctaa
     2521 gacgttgaaa agatactctg gcgaaatcgc ataccccaaa atatcaacga ttgttgcaat
     2581 cagatcttct tttgattctt catctgaaag taattcgcga tacaacatcg tttgtttgct
     2641 taccgtatca tattcctcta aggattcatc tgctaaaaga accacttgtt ccaacaaacg
     2701 gtcggataaa tatttataga aaatcagtcc caataaataa tttttatatt ctgacgcatc
     2761 cattttgcta cggagattat ctgccgcact aaataatttt tgatttaatt ctgcacccaa
     2821 aaagatacac tcctatctct tatttaaagt tgctatatac cctcttattt tagcatactc
     2881 ccttttagaa aaagcaaaaa aaaaaagaaa gaaacttatt cttgctaata aactacgcga
     2941 aaggagtcta acgttgtttg atataactta gactgatcgt taacttattt tttatatttc
     3001 tacatagtta tctttagaac catttcagat acatatgaaa caattacata tgattttttc
     3061 ataaaaaaac agtactgtct agtagtataa aatatacaaa tttcttaaaa attttttcac
     3121 taaaatcact acctgaaaca gtctaaaaca cttatatatc aacgtttaat gaaactatct
     3181 ccgtttcatt tcagatacaa ataaatatag tcgggctcca aatccgaaaa aaaatacccc
     3241 cagtagctta tcctaagcta ctggagatgt ataagtacgg tcatataaca acggggtagg
     3301 gtaaataaat gaaaaagatg gaagaatcat tattcaataa cttttttaaa tacttcgttg
     3361 taatcagtta cctgtccgtt cttaaatacc acagaataat cttttttccc attttcaagt
     3421 aaccgaagtg gaatatcttt accaactttc tctgtcactg cattactgat acctgttaga
     3481 ttagctaata aatctgataa agattgagca tctgtagaaa tctctgtatc agtatttatc
     3541 gtgattcctt tatcttcatt tacaagtttc caagtaggtg gtatagctgt gtatgtttct
     3601 acaaatgaat taagtttttc attaagtgac ttctctttta tatttccatc taaaaactct
     3661 gtatatatgg cttgagctat actaaaacaa agctggcctt gttctaataa ttttacatca
     3721 ttagttagta aagctgtgtt cacgttatca atcgcttcaa gaagagtatc tttggtgtcc
     3781 ctaacagctt taggtgtgcc ttctggaatt tccctacttg atacctcgtc cattttatct
     3841 tccataaacc ccttaaaatc atatctttct cccaaatcca tagaagtttt tgagaaagct
     3901 ttcatttctg agtctatttc ttcaataata tttaacatgt ctgatacata ttgtttaggc
     3961 tgattcgttt ttaatctata tgtggtaaat atctcaattc ctccaattat caataaaatt
     4021 gccaaacaaa tccataataa ttttttcttt ttcatttttc ctcctataca ctgctgttat
     4081 aacagtgact aatgtaaaga ttttcccagc aatcatcaga ctattattta cagggaatca
     4141 aaaaaactcc ttagtaagct tacaccttta ggagttcctg tttgaacggt atgcataata
     4201 catttaaatc aatttaacat taacacctca gtatatggta tctctacata tacacggtct
     4261 ttttttggat agtactcgac atttatattg atagcacttc cattgtctaa cgtagcttta
     4321 gcccggtacg tgttatcacc attatcatga gtgatcgtca catcatcaca tgtccgattt
     4381 aagttatact gttcttgtaa aatctgagtt accaatggta cagatgactt ctctagtcca
     4441 gatgaacccg tcattcttga aaaaattata cctataatag ccaatacaac gatacctata
     4501 atgacaaact tcttattcat atttacttta ttcaactttt tatctcctat ctccaaaatg
     4561 attttctctc atcagtaact cattgacatc cccaatagta caccaaagat ggctccagct
     4621 acccctgcta tcatcaaaat cgtacccatt tgttcatctt tctctcgata taagtaaccc
     4681 ataataacag caacagcacc aaataccaca ggaataaatg ctaatgatac taccatagaa
     4741 atccaaccaa taatcaaata cagcttccct gtctcttgtc catgtacgat ttgttccttc
     4801 atttcattaa ttgtctgctc agtacttttc tttactgtgt ctgttccggt tgtctcatct
     4861 gtttcttgta atgctccgca aaactggcaa tacttacttt catttgttat ttctttacca
     4921 cattttctac aatacatgtc ttctcctcct aaaatcattc aattgcttat tagctaactg
     4981 tttactttat taaatacatc agaaaacagc tcatttattg gtacacttaa tatctcagaa
     5041 agcattttca tttgataggg tttaggaatc gttctccctg tttgccacga acttacacat
     5101 tgctgtgtaa cattcatttt tttggctaac tcagtttggg aaagtccttt ttcttttctg
     5161 tatcggctga gattacttac ttttttcatg attctcccct cctgttttat tatatacaac
     5221 aatatgatgt atttcaactc ataaaatata taaatacttc aaatgtcatt acaaatacaa
     5281 caacttgttg tactctactg agaagggggg tgtattaatg aaacagaagc aatatgcaaa
     5341 ggtcattaga gaaaagcgaa aagcactcgg gtggacacaa aaagaattag ctaaaaaaat
     5401 cttctctaca caacaggcag tagcaagatg ggaaaattct gtaacagaac ctaacctcga
     5461 tagcttaaca gctctttcta gagccctagg aacccctgta agccattttc tagacaatgt
     5521 tgacatagat tatgaggaag agtttttggc tctctatcgc tctcttagta ctgaagatgc
     5581 aattcgaaca attgactata tgaagctatt aaaaaggcaa gaaaatgaac gcaatcagct
     5641 attgaaagat tgaaaaataa ataacccacc gaactttaac ggtgggtgta ttgagatgaa
     5701 ataagattaa gagctatgga taacctgtct agtttaatat aattgggtct cttctattcc
     5761 ttactctccg tccatcatct cttgttacac ttgtaaattg ataaatagga actaattcaa
     5821 ctcctgataa tatcaattct tttattactt catctatccc tgcccctttc gctagatata
     5881 ctaaaaaact actgcttgct ttcacattgt atagtttctt aatatcctta gaagctcgtt
     5941 caagagtgaa tccttgttta acaaagtagc ctctgttttc tattaaagca tttttcacgc
     6001 tgttatccct gacatttcgt aggcgttcac aaacagtgtt aataagttct ttttctgtca
     6061 ttagatatgt cccttgccac gataagccct gtaaaacttc atgtgcttca tctaaaatca
     6121 attgtttttc cttgctaagt tctgatcggg aattttgagg aaatactctt ttagcctctt
     6181 tttcgttaaa acatatctta acccattcat aagaaaaata ttgatatgta aaatgaaatt
     6241 tttccagaag tggaaggata tcttcattca atttttgaat cacagaagtt gcattaaaaa
     6301 cgtctaatcg atagacattg cttctcttat gtttaaagtt tcctaacgct ttctcttcct
     6361 ttaacctatt taggtattcg agcatcttca aaggaacatc ttcatcatct aattttttta
     6421 tcaatcctat aaaagtcata agtgatagta acttattaca tttatctatg tcataagttt
     6481 taccatagta agaactcatt tttttagcta attgttcccc cgacaaaata caagtcacac
     6541 tttccttact atcatcaaaa aaattaacat cagaaagcaa gacatcatag acacgtcgca
     6601 tttcctctag ataattcctt attgtcctga aaagtttgtc tttcattgat tcaagagtgt
     6661 caaaaagtac ttttgattcc tctttcaatt ttcgaaaatg tgtaggatac aagtctaggt
     6721 tctctaatag ccaattaaat gtttctaccc gactgtcttt ctttaaaatt agcatcacaa
     6781 ctccaataaa attttcactg atatcacaat tactggaatg acaataaaaa atggaatgcc
     6841 cagttttact ggtaaaaata ctagcagagg gattatgatc gttgtgaaac agacatctta
     6901 agtttttact tgacttaagt aatttattca tatcaatggt taacaatctt tcataaatct
     6961 cattaaaaga catgtcggag gaatcaaaca ggcagtcttt aaaccactgt ttcatttctt
     7021 ctttgtcgtc attttttatc aacttaacaa aatcggtttc attgactagt tcatactctc
     7081 tccctgaatc actttgacaa tttttagaac atgtcatggt aaaatcaatc ggaagctcag
     7141 taatatctaa catgttgtct tcattaatta ttatcactgc atctgactta tatcctccaa
     7201 aaaacattct attcgaagca tttacattgt catcacaccc ggggatgttc tttttcaagt
     7261 attcgtaaac agcaaccatt ttaccatagt cagtaattgc ttcttttaat agaaagacaa
     7321 tcttgtattt ttctagattt tcagagtacc gaatactttt ttgtatcgca aacgcagaat
     7381 tatgaactaa attgatagat aaaacctttt ctatgcttaa gtatctacta tcatctttac
     7441 tcaaatctac taccttcccc ttctcatctt taacagtgtt atcgacatcc agaataatta
     7501 ctctggtttc cacaaaactc tccttgtcat tgcctttatt ctttgtcttg cttggaataa
     7561 atgattgtga tgaatttagt atctctacaa actcatctct tgtaacttct ctatagttat
     7621 ctaaacacct atctcttaca tcagtttttt ctttttttgt aactggcttc tctaataatt
     7681 tttctttatc tacataaatt ggaatattaa tcattgatgt ttccatttgt gtttcctcct
     7741 tataaaattc tgttaaagac taaaacatac agataataaa aacaaactct ttattactat
     7801 atgttttagt cttatttgtt tactcaacct ttataccgtt ccatgaaact atcaatatca
     7861 gattttttaa atctaatgac cgtatcaatt tttataacag gtaacccgta ttgagaaaca
     7921 tattttctaa tcgttcctac tgatacacct agatattcac ttgcctgctg atagtccata
     7981 tactctagag tgactccaga aataatgtct tctagcatag gctttagcac tgtaattatc
     8041 tctttcactt gttgttcaac ttgtatagaa acaatgctat caatgatttg tagagacata
     8101 tttccccctc ctttcgtgat tgcttgaaca aattttctaa gaattatctt cgtatttttt
     8161 gctcaaaata aaagtatttt gttattaacc gattaatcaa acaaaaaatg attaccagtt
     8221 catcgacata cacataatac ctcgataaaa aaggggtgtc aatagttttg taaaatttat
     8281 ttacaacacc ccttttttga ggtatactaa ttttaggagg tgtggtcaat atgataaaac
     8341 gtccaccaat aaattattta gaacgaaaaa agatactcgg aacaaaaata aaagctataa
     8401 gaaagagtaa aaaattaaca caaccagcat ttggtcttat gattaacaac ggtcaattaa
     8461 ttgacaaaaa gactatctac gaatgggaga agggaaccta tttaccaata ccagagcgat
     8521 tatcccgaat tgctgatttg ggaaatatga gtatagagga gttagtttgt ggcaatgtag
     8581 aagaatatat tttgggaatt attctttata gagatagcat tgtactagat ggtataacat
     8641 ttccagataa aaacctgttc cagcatcttc gtcaacagtt cccaccagta cactctaatt
     8701 tagatacatg gctggataga tattccaaat tagaaccaga gatgcaagaa tttattgcta
     8761 acaagacttg taataaagtt aaaaatgaaa aaattagtct tttcaatatc ttaaaaatag
     8821 aagaattatt catcaatgct attgtagaag aatttgacaa taacattctt ttcctcacta
     8881 gcagtataga agaactacta gaaagaatgg tagatgaatg gctacctatt caattgaaag
     8941 acatgtctta tcccgaagaa gctgtgagag aaattactga taatataaac aagctagaac
     9001 aaacgatttc atcaattggg aaaaaataca ctaagaaaaa aatgaaggga ggtgatacca
     9061 tctagtggta agtactcctt gtaaactaag aggagatgat ttcatggcaa aaatatcaaa
     9121 gtacaaaaag aaaaacggtc aagtggcgtg gatgtataaa ggacacattg ccaccgatcc
     9181 acgaactggt gaaaaaataa atactacgag aagaggtttt agtagcaaac aagaagctca
     9241 aaaagattat gacgagtaca gacatcaaat attatacggt gttaaaaaga aagcacctga
     9301 tatgacattt gaagacctgt ataatgaatg gataactcat cagcgtacca gtgttaaagc
     9361 ttcaacaatc gcaatctcag taagatacgc aaataatcaa atacttccag catttggtaa
     9421 gcttaaacta tcgaatatct ctgtcccata ttgtcaaaaa gtggtagatg aatggcacag
     9481 taaatatgaa agttacgatt atatgagaaa acagactgca caaattttac gctacggggt
     9541 tgcaatgcaa tatattgaca ataatcctat ggaaaaaaca cttcttccac gaaaaaaaga
     9601 gtatgagaaa aatcgtaaat tttactcaaa agaggagctt aataatttac tggatgcttt
     9661 taaagatttt ggtaacatga aacaatacgc cttcttcaga ttattggcat acacaggtat
     9721 gagaaagagt gaagtactcg ctttacagtg gaaagacata gacacgttta ataaagaact
     9781 acatgtcaat aaaactcttg ctgtagatga atttggtaaa gttataattc aatctccgaa
     9841 aacaagagct tctagacgag ttatctcttt agatactgag actctttcta tcctcaacaa
     9901 ctggaaatta cagcaaaaag aagagtattt aaaacttgga tacaatacta gctctaaaga
     9961 gcaacatgtt tttacaacag ttaaaaacac tctatacatt ccaaacactg ttaatgattg
    10021 gttaagatat attttgaaaa aatataactt gcctcgtatt acacctcatg ggtttagaca
    10081 tacacatgct agtttattat tagaagcagg agaatctgtc aaggtagttc aacaacgatt
    10141 agggcatgaa aatagtaaag tgacattaga catctatgca catattacaa ataatgcacc
    10201 taagaaaaca ggtcaagact ttgctgatat gatggctcat cagtaaaaga gctggtcaaa
    10261 atctggtcat ttctagtata caggcaaaac aaaaaagcta gaacccttat atatcaaggg
    10321 ttctagctcc tataacaggt ttacccaaca ctgccttcca atgactcttt tgggaatcag
    10381 aggaaagaat caggctttat agagatattt catctaacaa attactttag actgacagta
    10441 tatatcagca ggtactgtca tcgggaacgt agccaatatc gtatccaaaa cagcaggtag
    10501 agaagctatt tgaggattca agttattcta tttcaaccat gaagttaagt tcattaaaaa
    10561 taaagatagt tggtatttca ataatttttg tatagagtta atcactttat tgatgctaat
    10621 tcatcttgat tggtattata atcgtattca agttgcgtgg cgtgcttttg ctagaaaata
    10681 tataactgtt ctttataaag aaatataagg attaaacatt ttttatcata gagttttact
    10741 tatctataga attatgtctg ttcctaattc agtttgatga attaactgat aaaaaaatat
    10801 ctactattta aagatacagg gataagtaag taaaaaaata acatgtcatt agatagtaga
    10861 cagttccaac tctttattta ccttttttgt atgctgcact gtcaatccaa gttcctaaat
    10921 tgttgtatcc atcatcactt atataatcat attggccatc aataatttcg tcaaaccaca
    10981 aatattctcc atctggtttt tgcccaatta ttttaattac tgattgacta gtagttgtca
    11041 tctggttaaa atcttttatt ttacctatat taacggccaa aatagcattt cctcgcttat
    11101 aactctcctg gatttcatat ttaacatatt ctcgatcaag agtttcttta cctatcagca
    11161 ccacagtcac tgatgttcca attaattgat tatctatcca gttttttata gcaatatctc
    11221 cattacgttt aactttctca aattcagcct tatctacaaa accagcttcc tttgttcctc
    11281 gcaaagtcca gctgtttcta actaccattg ctcttgttac atctgcataa tggaaactaa
    11341 aaaaaaccct tctcaccata ttaatcctcc tgaatactat ttattaaact gacaatttgt
    11401 tcaacttttt tatctacttc tccttcagta tctaattttt ctagcgcttg aaatttactt
    11461 tttaaggaat catcaaaata atcactgaaa ttctcttgaa tcttatcaaa caaaacctta
    11521 cttgcacctt tggtaaatcc aatcggaact actatatttt cattttgaac agataattca
    11581 aattcttcaa tcattccatc tgctgtaact atttcacctg attcaagttt gtttccaaaa
    11641 acaaaaatac taatcccaga ctctgcaata atgttttctc gatatcttct ccactcatga
    11701 ggcatatcaa ggggaagtgg tcttaaagaa agtacgtctt gtatgttatt ttttcttaaa
    11761 tttcttctct gctttagaac tcctgaaaca atactattac caattccaag tccgtaacct
    11821 gatactattc tataaccgga ttcagaaagc ctatatgcta atttctcgac aaattttaat
    11881 cctttcgatt cagatgtaaa tggagtataa tcaacagcac ttcccgaaat aaaaatagtt
    11941 tttctcaaat acttttttct aatacttttc aaaatatcag taatctcaga ataatcatct
    12001 acttcaacaa cgtttatcga aaatctggac aaatcattga tttgtagtct tctttttgtc
    12061 agttcataat tatatctctc ttgatcaaaa aaatcctcag aatcgacaaa attttccttt
    12121 aatatttttc tcattataca ataatgaggt ctttggtttt ctctcaaaac ccatcttata
    12181 tctgataaaa ttctttctaa attagggtct gtaaaactaa atcctaaaaa gagaaaagtt
    12241 tttgttaaca aatctccttt taaagtttct ttaaataacg cgtacgaatc atcatcatat
    12301 ttttcataat catctcttgt tattactgtc gaactgacat catctatatc accatgcatt
    12361 ttataaacaa ttgtatccac atttggatta gttgttgcta actgagtata atttttctta
    12421 acctgagggt ttttataagc ttctcggatt gatttttcta ttagatgatc ataattagtt
    12481 gtccaataag tttcaatagg taaacttgct agtattcgat gattttcaga tatttctaat
    12541 tcttcaccaa aattatccat aagtatctga ttcaaaattc ctcttgatcc accattttca
    12601 tttttataat attgagccag agtaactaag tctgtttctt ttttagaatc caacccgatt
    12661 tctgttgcag gatcattaag tagttccggc caacttacat atccttgtga catcgataat
    12721 cctgcacctg caaatattgc tgcatttcct ttttcaatag cttcagagaa cctctttatt
    12781 aaaactttct tatccattct acccggttcc tttcgattca gtaaattgat tagagattag
    12841 tgaaaatggt gtttttatat aaaatatcta atgtattact tcctttagtg cattccacag
    12901 catctaaagc ccattttttc aaatcagctt ttcgtaaata agttaattca cttaaattta
    12961 aagaatattt tatttgcaga ttttcttgcg aagaatagtg ctttataatg cttctaacat
    13021 cctcggtgct catttgagga ttactttcca atcgtggatt ctttttttcc actattgaag
    13081 ctgtcgtcag ttcgctatat agccatggcg aatacgtaaa tttctgttca ttagtgattt
    13141 cattgctcaa actaattgaa ttaggtgtat taagaaaaaa cagacattct gtactatcaa
    13201 tcatattatt taaagctgta gaaagcatca tatgaacatg actagttgtt acatttcttt
    13261 tatcataatc atatgttttt gtttgttcct tatagcaata tttttggtct atttttttta
    13321 ataaatcaga actgtatccc caaaccgaag aatcaataaa tgcagtaaga ccaaaatttt
    13381 caaatagcca tgatgcaaaa cgtatagcta atttttcatc agcatgagag tgagatataa
    13441 aaatatctgc ttttatagtt ggaaaccaat ctttaataat ttcgtctgca tctagaattt
    13501 tgctgtcctt agaataataa tcaactaaat cattttcact ttcaaataga ttttctattc
    13561 tcttgttaat agatttttgg ttgtgatcta tttgatttac tacatgtgta tatgattgcg
    13621 agcttgagga gaatgcattt aagtcagcat ttgtaaaatc caattcatat gccttataca
    13681 aggatatccc tccattcttg tattttgatc cacaatttat taggcaacat aacagtatac
    13741 cctatcgtta catatcccat tcattattat accagtattt cttcgtgaca cataagggtg
    13801 taacataata atttcaaacg aaatgactac aagtagtcat tgaagcaaat cttcataaga
    13861 cacctgtaga atatctttga tggctttcaa ttgatccaac ttaatgtgtt ggcggccacc
    13921 ttcaatctta accaacgttt ccctagttat agcaatttta cgaagctgta ggtctctaat
    13981 cagctcggtt tgaccaattc ctttttctaa tcggactcgt ctaatattgc taccgattac
    14041 attttcttta tcaataattt tttcctcttt caagaatagc acaccttttg gactaaaatt
    14101 agtccttttt cttattaatc ttaaagattc ggtatgctac aatgggacta gtattagtcc
    14161 atattcaatt ttaaggagga atttttttga agaaatttgc aatcattcta acttgcgctt
    14221 tactcatcgg aactataagc gggtgtacca cttctgacac aagttctaca agtacggaaa
    14281 ctagtacaac aacctcagaa aagaattacc caatacattt ttttccggtt ccagatggat
    14341 ttgaagctta ttatcatgat aaaaaactaa atgttccttt atcagaaact gaatctactg
    14401 aaaagatttt gccaattgaa attgaaagaa atcaagacga tataaaacta aaagctgatc
    14461 ttcccttact gaacagcaca caaattgata gacatgaaaa tattaaagtt aatattgaaa
    14521 aaggagcgtt agcacaactt tatactgaaa aagttgaaca attttttgag gctggtgcca
    14581 ataaagactt ttcaaaaata agcgattatt cggataactt ttttaatgag acccaagatg
    14641 aaatcacgaa ctcaactttt atggctgaag atatgtacaa tatgaagttg aaaaaaataa
    14701 agtcgtatga ttatccatta aatctaacat taaataaagg gatactctct tttgtattag
    14761 caggttacgt tgactatgaa tatacagcag attatttgga gacgcccctt caacacgata
    14821 ataacgatat taattttaga tttatctaca attcaaaaag taaaacatgg ttagttgatc
    14881 aattgagcac tataaatacg ggatttcatg aattacctaa gcctgacaat gaaactgctt
    14941 tggtcattaa aacattctaa aaaagaaacg ttgaaggaat aattattctt tcaacgtttc
    15001 ttttattgtt tttcagttta cggtcttacc tgagcgaata atctgatttc taaatgattg
    15061 gaaaacttct tgtggttgat aataatctgt atctatttca aatgtttctg agaataattc
    15121 attgaatttt tctactgaaa tgatgatttt accattctcc tctcgactgt tttctattat
    15181 ttctgcctct aattcaaata aatctagtga cgaactaaaa tcataaatcc tgtcatattg
    15241 aacaaaaaaa tcaagaaaaa gttgctcaaa ctcttcctta tttatcacta gtgtttcaag
    15301 cggaacaact ttattttcat cagccattct tccaatttcc tcactttcat tttcaattgt
    15361 ccattgttgc ggacgtatca gattttctct atcatttttc agtgtataga tcacttgtaa
    15421 tacacaacaa tgctttgtaa acttattttt ttagttagtc gaatcttttt aaatgtgcgt
    15481 ttcataccat gcgcttgata tattttctcc tattttttaa atcgtttatc acaactttta
    15541 tcgttttttc ttctgaaaaa aatcttgttt acatcgttac atagtttcca aaaagataca
    15601 ttactagtag aaacaacaag attaatttta cttcccactg aaatgggaag tatattttaa
    15661 atattttttc tccttcaact tacactataa agtatcttaa tcacacgata tgctagccac
    15721 taaattcatc atatttgatt ccttttttaa gataatgtag catcttttta tccaataacg
    15781 caattgtttt taaggataaa tttcttaaga tggtacattt caaaatggca tttgacatat
    15841 gaaacaaaaa agaaagaaaa gagcataagg gaaaactgat agcaccttcc tacaggaagg
    15901 tggctaagtt ggctgtgcca actggttttc tttcaaaatc atttcataat ttttgatatc
    15961 ataaaaaaat ccattttgga cctattttcg gtcataattt agtacctact tttggtcata
    16021 gtttcgtccg tagttttggt catagtgact aaggcttttc tgttcacaaa attggtttaa
    16081 atttgctttc tttaacactt catttatatc atagcaatta taatgtttac acaacatcat
    16141 tcctaatttt aacggttgtt cctctttata ttttttaagt tctacatttt tttgcgttta
    16201 attacttttc tcttttcttt atattccgct ccaactttct ctttaatagt gataatagaa
    16261 ttacatattt ttgttaaact tccttaaaaa aattggatct cgattactca aatttgcttg
    16321 caggcttttc taattgcttt gttttttctt cctaattatt tgattttatt tccattttct
    16381 tctagtgaaa atttattatt tttaataatc gtacgcgtag aactgatttc attttttgta
    16441 tctgtttgat tatctaacgt ttgaaattca aagttttgat tggctttatc aatatctttt
    16501 tatactactt tatttgcaag atatattttc ttgcttaatt tggtcatagt ttcattcgtc
    16561 acttcggtga tattttttta atttgcaaat aaaaagagtt aaaccgatat acattaggtt
    16621 caactctttt gcaggtttca tgggtgtact atcactttta atctgataga tgtaaacctc
    16681 tctttatctt tttgttactt caattttcaa caatagctcc tttataggtt gtcaatattg
    16741 ctctacgtat tctggctttt taacccatta tttctctaaa gtggaagaga ataatcgctt
    16801 aatccccact aattccttgt cgtaaattca tgtcaaatac aatttttaaa agccatttaa
    16861 ttcatggatt atctcttcac ttttttctag ataacaatat ttcaagttgt tttttatctc
    16921 atgtttcatc tttaattctt cttgaatcat cagttcaata taattagtga ccgtgatttt
    16981 ttcatttgtt gcgttcttta ctgattcaca taattcgtca agttctaaga aatcatgcgt
    17041 actaatcttt agaattaatt tcatgacaat tccccctttt atttttttaa tagattatcg
    17101 aaaagcggat taggatcatt aagtgtatct ctgaataact cgttgtcttc ataggtaaat
    17161 cttcgtcttg gttttgatcc cttatcattt tcacggattt cactatcaat aatcactgtt
    17221 tcatgatttg aatcaaattt tttcgctgac tttctgtctt caacgatatt gattttttgt
    17281 gttttttctt tcatttctcc actatctttt tgaacgttct cccgaacatc tactgttttg
    17341 aatccttgac tacttgtaga actttgtaca ttctctctta catccattga tttaaaccct
    17401 tgactacttg tagaattttg tacattctct cttacatcca ttgatttaaa cccttgacta
    17461 cttgtagaat tttgtacatt ttctcgaaca tcaattgtct tagtaccttg accatttgaa
    17521 gagctttgta aattctctct tacatcaaca ttccgtccga catgaccatt caatgaacct
    17581 tgaatatttt ctcggacatc tactgatcgg ccaccttgac tacttgtaga attttgtaga
    17641 ttttctttga ccaatttttc taatccggta tttgagtttt tagcttcttg gattctttct
    17701 ttggtcaacg cttctttttc gtccgtatta gctcctgtac gcccttctac acgtttcctt
    17761 aacatttcct cacgattcat tgcttcaggc gtgtatttag cttcttgtcg cttctgatta
    17821 attaattctt cagcagactt agcttgaccg tgcatagcat ttttacgttc ttcaatatct
    17881 tgttgaactt tttgctgaat tgcacttaca ggattattat tcgattcagc attatttttc
    17941 attgcttcac gcatacgttc agaactagac ataattctat cggcattttc aaccttagca
    18001 ccatcttttc tatccgaaag acgtgttggc atggaatcag catattttcc agtttcatct
    18061 tctttatttt tagctaacat aggtttgcta tttttattaa tagcggacac tgtaccatca
    18121 tctaaacctt ctttgaactt attcttggta tcattagcca tatcacgcat actcttaaat
    18181 ggttttattg ctctttcact agccattgta gctccatctg aagctaaacc tgataatcca
    18241 cgttcatggg catatcccaa agctcttcca gttttgccag ctttttttga tagagtttcg
    18301 ccgaaattct tttctggttt tcgatcttct ccttctccac tgtctgatcc atttccattt
    18361 ccgcttcctt tagcacgatt aaatccagtt gatccttttc taaacagcat tgcgcccata
    18421 ccaaaagcag aagcaagttg cccatagcct tctttgagtc caatatcaac accaaagtat
    18481 ctcatgactg ttccactgcc ttttatcagc acaaacacag cacagatata agcaattgtt
    18541 tttataaaaa tatttgtgga gataccttgt ccagcattta aaaaattgat aaacatagca
    18601 aacatagata gtccaaaccc ttgaaatcca actgttaagt aacattgtaa aatatcggat
    18661 aaaacaactt ttgaacgttg tcctgtttca atatctgtcg caaaaacaag aacacctaac
    18721 acacgtttaa atgcaagttc taatattgct gtaatgatca caaacgcgct gaacacatag
    18781 gcgattgcca acgctgtcaa cccaatcgaa atcccccaaa gatttgcttg atatcgataa
    18841 taacctgatt tcaagctatc agaaaatgtt gataagaagt tatcagaaaa ctttgtggca
    18901 acaaattcat tattagaatt ttcaactaat tcatagcgaa gattatccgc agctggattt
    18961 tctttttcca atttatcaat cactttatcc gtcaacactt gtgttaaatc tgtctgttga
    19021 aaattttttc gtgttaactt atttcgatcc tccgttttat caatgcttga gtattgatct
    19081 gttttattaa tatatgccag atctgttact ccttgtttta tcaatgacca agcgactccg
    19141 tcatctgaat ttgtaatcgt agtagcatca ttgtaaaata ttttggcaaa ttgaataccg
    19201 ctagaaatca ttgttggcat taaaagaatt aacgctacag acatcactat attcattcca
    19261 acagatctta aatctattgt tccttttcca gtaatcatct tgtagccaac aaacatcaat
    19321 gaaaggatca ttaatccaac gacaattgta tttatgactg attgataaac attatttagc
    19381 cctgtacttt caatgaaatt gaacaaatcc aacacatctg gaatcatatt ttgaatcgaa
    19441 tcagtcacag taaacatgcc ttttaccaca gcccatccaa gccaaatccc gaactgacta
    19501 aggaaatctc cattgtattt ttttaggtaa ggtctataaa gagaattatt caaaaactct
    19561 gtttgacttt ttgtgtcatc aaaccaattg aaaatgctag cttcaacaat attacttgta
    19621 aaaacaacgc taatcattaa aacaagtaca ctgagtgcta accagtttat ttttctacta
    19681 acgatttttt ctttaattgc tttcatagtt ttctccttca gcccattttc ttaggctctc
    19741 agagagctgc tgagccattt tttgattaat tatgatatga gttacacaat tgtcattttc
    19801 agctcttaaa tcgagaacta ctgtgttgaa gcagatactc tcgattaatt cgagagtatc
    19861 tgtattttga tcaagacacc acatttgatc tattaagcga tttttaatca tgattcttct
    19921 cctttcaacc aattatgcat agtaatttca ggtaactctt tgatttcgaa agcttttttg
    19981 aactgatcta taggctctac attttctaaa aattgacttt tatatctttt ctgaagtcca
    20041 ttcaaaaaat atgttacata atcgtataac ccttcttcag tttttctttg aaaaaagcat
    20101 gcttcatgtt gttttataat acactcagca ataagatact gagtattttc agataaatat
    20161 tcaatgattt ttctgatatc ttctgtatct acattagcgt ttctcaaata gtgatacact
    20221 tgaaataatt ttcgaccatc ttcgttattt gcgtcataaa gaacactaat cttactgtaa
    20281 atcctcttta tattaatatc ctcttcttcc ataacgagag tagtctttga tatgttctgt
    20341 gaagtagtct ttgtatacgt atatacaaat ttgtacatac ctctaccttt ttttaaagag
    20401 acatatctct ttttttcgat acccctctga cgaattttcg gcatatactc ctttttttcg
    20461 caatccaaag taggaaaagt aatttctatc aatttatgta catttaaaac aatgtacata
    20521 acatttgata atgtaactgc tcttttctca taataaatat ttctaaaaac ccttttgata
    20581 atcccctttt tctctaagta cacaattgca tcttttatag ctcgttctga taatcctgtt
    20641 tttttgctta attgcttata tgatttttgc aaaatatctt caagaaactt tttttgataa
    20701 ccaagtagct gaccatcatc gttaaaaatt tttttaggtc tataccaata ggtaatttct
    20761 gctaagataa ctattgcatt caaatagggc ttctcaacac cttttttcgt tttttttctt
    20821 aattgatcgt accatgtgtg tggattacat ttccgtcaaa atttatttct ctaagtttgt
    20881 caactacaac atttccagaa gaaaacacct tttcaccttc ttttttgata taatagctat
    20941 aaataaagtt ttctcagcac tttattattg agtgttcccc cagtctgcaa acttttggga
    21001 acactttttt tcttttttta aagcattttt ttataatttc tacttcatca tcttccaata
    21061 ttttcccctc ttttcattcg agcaaacata aactcattaa ctgccttttt cgaataaaat
    21121 tttcttgccc ctaattcaac tacgggcaat ccctcatttc tcacagcatt ttttaaaaag
    21181 ttttcactca catttaagta ttccgccatt tctttttgat tgagatattc ccttgtcatc
    21241 cccgcatttt cttgagcctt ttcaatagct tttgtagcaa tttgataaaa ctgctttaga
    21301 aacatttcct cctgttcttc tgaaatcatt aattttattg cttccacaaa aacctctcct
    21361 ttttaattaa ttattctata actcctctac ctttataagt acaaataaat acaaattgcg
    21421 taaaaaaatg tatttatttg tatttataag aataaagtat catactccag ataaaaagta
    21481 aatactttta aatactttta ttttataaat atgatatact atctttaaaa ataaaaagga
    21541 gaaaaaagat gaagccaaat ccttcagaag caggaaaacg aatcaaacaa ttacgccttt
    21601 catgtggttt tactatggaa gaacttggaa ggaaaataga taattcacca cgtgcgacca
    21661 tatccaactg ggaacgtggg accaatttac caaatccaca aaagctgaaa ttattatcaa
    21721 ctatcactaa ttcaacaatt gactggatta aatggggaac tttagaagaa tatataactt
    21781 cataccttat cgatataggg tatgagctat atattaagga cttcccagaa atacctcaca
    21841 aagtctttaa agacattcaa gaaagatata gtgatacgtt ttcgctagat aaagactatg
    21901 agttattgaa tcctataata aaaaatatat ttacaaaata ttactcaaaa gattttgaaa
    21961 tttatctaaa tgagatactt gctaataata tcaatcctct gattgaagag tacgctaacc
    22021 aaagacactc tatctctaaa gagctatttt tcaaaagagc caacagtaaa cttcaagaca
    22081 agataagaaa tggtgatttc aaatacggtg aaaaagaaag aatgattgat tctttaaaac
    22141 agctattaga cgaaatggac ttagcctata aagatatttc aaagtattca tctgtcgagg
    22201 atttctttta taaaattact aaaaaccaat ttgaaacaga aaaatttatt tcagatctta
    22261 gtaaaaaata caatttccca tatgagaaaa atagtataat cgcaaaattt ttaactgata
    22321 atcacgataa atttaaaaat aattgattct tcaaataact tctctacctg caataatgaa
    22381 gtaaggagag aatcaaatgg caacattttt tagttataaa ttacaaaatg gcgaaaaata
    22441 ttggggattt gaaacttatt tagggaaaga tcccaagact ggaaaagata tacgtacaaa
    22501 gcgtagaaga ttcaaatcaa aagctgaggc gcaaacagca cttaatagac tacttgtaga
    22561 ctttaaagaa aaacaagagt ttaaaatcaa tgttacaact tttcaagagt tgtatgaatt
    22621 atggattgat aactacagaa caagagtaaa accgtcttct gttgccgttg ctcaacgttt
    22681 ttgcaaaaac catatattac ctgcttttgg ggaattacga ttggataaaa taagtgttag
    22741 ctattgccaa aaacaggtga atgaatggca caaaacatta aaacaatatg gtttcttgag
    22801 aaaaacaact gctcaaatat ttaaatttgg agtagcaatg gaagtctgta aggataatcc
    22861 tatgtctaaa acattgcttc caagaaaaat tgaagaagaa caacccttga agttttacac
    22921 gaaagatcaa ttacaactgt ttttacagaa tactaaagaa aataattcag ttaaactgta
    22981 cacttttttc cgtttattag cttatacagg aatgcgtaaa agcgaagcat tggcgcttca
    23041 atgggaagat atagactatt tcaataaaac aataacaatt ggtaaaacta tcgctcaaga
    23101 cgagtttaat caagtagttc tacaagtacc taaaactaaa aattcatcaa gaacgattca
    23161 gcttgatgat tttacactta aacaactgcg tatttggcaa caagaacaaa tgaaaataat
    23221 gatcttatat ggctataaca cgaattcacc aaaacagttt cttttcacta caaacacaaa
    23281 caagttgtac tacccccaag tagtaaacga ttggttagac tggatttaca aaaagacacc
    23341 gatggagcct caaattactc cacatggatt taggcatacc cactgtagct tattgtttga
    23401 aagtggtgcg tcgataaaag aagtacagga acgattaggt cataaagata ttaaaactac
    23461 catgaatatc tatgctcatg tcacaccaca atctgttaag aaaactggtg accgatttgc
    23521 aaaatttatg ggcgaataat tccaaacgta gccaaaaacg tagccaacca ttaaaataca
    23581 aataaaaaaa cgctcgaaac gctttaatat caacgtttcg agcaatgaaa tagttattaa
    23641 cctacagacc cttccatctc ataactgatc aatcgattga gttcgactgc atattccatt
    23701 ggaagttctt tggtgaatgg ttcaacaaag cccatgacga tcatttcagt tgcttcagat
    23761 tcggtcaggc ctcggctcat cagatagtag agttgttctt ctgagatttt tgagactttg
    23821 gcttcgtgtt ctaaagagac ttggctgttg tggatctcat tgaatgggat cgtatcggat
    23881 ttcgaccatt cgtccatgat gatcgtgtca cattcgatat gagagatcga acccgcactg
    23941 tttttggcaa aggttacttg tcctcgatag tttacttctc cgccgtcttt tgaaattgat
    24001 ttagagacga tagagctaga tgtatttggt gcattgtgga tcatttttgc acccgtatct
    24061 tggatttggt ttgctcctgc aaaggcgatg gagagcatgg tgcctcgtgc accttttcca
    24121 tctaagtaaa cacttgggta tttcatcgtt gtttttgcac ctaggtttcc gtcgatccat
    24181 tcaacggttg cgccttcgta tgcttttgct cgtttggtta ctaagttgta cacattgtct
    24241 gaccagtttt ggatcgttgt gtaacggcaa tacgcatctt ttcttgtaaa gatttctacg
    24301 atggcggcat gcaggctgtt acttgaataa gtaggtgccg tacaaccttc gacatagtgg
    24361 atgcttgctc cttcgtcaac aatgatcagc gtacgttcga attgccccgt gttttccgca
    24421 ttgatccgga agtacgtttg taatggaaca tcgactttga cgccttttgg cacgtaaata
    24481 aaggtcccac ctgaccatac ggctgaattc agcgctgcta atttgttgtc ggttggcggt
    24541 acaagttttg aaaagtactc tttgaataat tcagggtatt ctttcaaagc agagtcagtg
    24601 tcagtaaaga cgattcctaa cttttcgaat tcatctttca tattatggta cacgacttct
    24661 gattcatatt gtgcggaagc accggctaga taagctcgtt cagcttctgg tataccaatc
    24721 ttttcaaatg tatctttgat tttttctggt acgtcttccc agtcacgagc gggtttgtca
    24781 cttggttttt ggtaatattt gatggcatcg aaatcgatgt ctgaaaggtc ggggccccat
    24841 gtttgcattg gcattttgtt aaatgtttct agagatttca gacggaattc aagcatccat
    24901 tctggttcgc ctttgattct tgacatctca cggatcactt cttctgtcaa gccttctcct
    24961 gtactgaaaa ccggttcgac atcatcgtga aacccgaatt tatattcttc taattctggt
    25021 acgcccatgt tctcactcct cttccttgtg gtaatgatgg aaagctgcgg taccatcgtt
    25081 ttccattgct tttcctagtg ctttccaggc aagtgtcgca catttgatcc gagcgggaaa
    25141 tttagctaca ccaccaagca tagcagcatc gcctaattgg tcgggatctt tgacttcttt
    25201 tccttggaca agctgtagaa agtctacagt taaatgttct gcttcttcta ttgattttcc
    25261 gatgacggcg tctgtcatca tcgaggcact tgccgtactg atggagcagc cactgccgtc
    25321 aaaggcgata tttttgatga ttccgtcttt tacatcgact tgcaattcga tcacatctcc
    25381 acatgtgggg ttgttcaatt cgatcttgct tgtcgagtca gtcaagcttc cacgatgatg
    25441 cggatgggaa gaatgatcca agatcacttg acggtataaa ttgtctaatt tagatagtcc
    25501 catgttggaa aaactccttt gttgccatga ttgcttcaac taaacggtcg gcgtcttgcg
    25561 ttgtgttgta gaaataaaag ctcgcgcgag cagtcgctga tacttcaagg tacttcaata
    25621 gcggttgcgc acaatgatga cctgctctga cggctacccc ttccatgtct aaagctgtcg
    25681 cgacatcatg gggatgtaac ccatccaaat tgaatgcaac gactcctgtc cgaaaattcg
    25741 ggtcttgtgg accataaaca gtcaatccat caattgcttg taattttggc aacacataag
    25801 cggctagttc ttcttcatat cgatggatat tgtccatacc gagtttttgc aaataatcga
    25861 ttgcatggcc taatgcgata gcgccggcga tatttggtgt ccctgcttcg aatttccaag
    25921 gtagttcttt ccatgtactg tcgtataatt cgacaaaatc gatcatttcg ccaccaaatt
    25981 caacaggttc catttcttct aaccattttc gtttaccata taaaacacca atacctgttg
    26041 gtccgcacat tttgtgtccg ctgaaagcaa agaaatcaca gtctagtgct tggacatcaa
    26101 tggccatgtg cggggcagat tgagcaccat ctactacgat gaccccgccg ttttcatgga
    26161 caagttcagc taattcttga acaggattga agacacctaa gacattggat acatgagcaa
    26221 tggagacgat tttggttttg tccgtaatct ttttcgctgc atcctccata tccaggaagc
    26281 cttcttctgt tagttcgata tatttcaaga cagcacctgt tcgttttgcc aactgctgcc
    26341 acgggataac attagaatga tgttccatgt aagagagcac tatctcgtct cctgctttga
    26401 tatagcggtc gccgaaggtt ctggctaccc aattcaagct tgtcgtcgta ccacgagtaa
    26461 acagcacttc tgctgtttct tttgcatgga tgaacgcccg tactttttct cttgcttgtt
    26521 cataatcatg ggtcgcgcgt tctgctaacg tatggacacc gcgatgaaca ttggcgttat
    26581 cgtgttcgta atagtacgct aatgcctgga gtacttgttt tggcttttgg gtcgtcgctg
    26641 cattatccag ataaactaaa ggttcatcat tgacgtcttg aaataagata ggaaaatcat
    26701 tgcgtagctt tttagcatcg atcatccatt caacttccct tcgattactt cgactaattc
    26761 tttccgaact tgttctacag ggattgctgt taagacagaa ccgaggaatc ctcgaatcac
    26821 taagcgctct gcttcttctt tatgcaatcc tctactcatc agatagtaca tttcttctgg
    26881 atcaactcgt cctacactgg cagcatgtcc agcagttact tcgttttcat cgatcaaaag
    26941 aataggattc gcatcaccac gtgctttatc agaaagcatc aagacacgac tttcttgttg
    27001 ggcatctgca ccttttgccc ctttcaagat gtggccgata ccattaaagg tcaatgttcc
    27061 tttttctcgg atcactccgt gttgcaaaat atgcccgatc gtatgagggg ctttattggt
    27121 tactcgtgta tcgattcctt ggatttgtct gcccgagctg attgccacga ttttgacttc
    27181 tgcgtgtgca ccttctcctg ctaaatctga gtcaaagtca gcgatcacat gaccgtcatt
    27241 catgacacca attgcccagt cgacagaagc atctcgtaaa atgtgcccac ggcgattcat
    27301 ataagaagta atattttctc ctaattgatc tacggctgaa tatttgattt ttgaaccagc
    27361 tttagcgatg acttccacga tgatattacc tgatgatttt gctacttgtt ctttggttgt
    27421 ctggaaacgt tccagataag aaaattcact atgttcatct gccacgatca acacatgctt
    27481 gaagaagtgc tcatcggatg cgccatcttg aatgaataag gactcgagtg gttcatcgat
    27541 cacgacattt ttaggcacat acaggaaaat accgctgttc ataaaagcta catggagtgc
    27601 agtcaattgg tcttcgttca tgtttactgc ttttttcata taatattctt ggactagttc
    27661 tggatattcg atcattgcag tgaacaagtc ggtaaaaatg actccttttt ctgctaattc
    27721 cacaggcagt tgttcaaaaa tcgtccatga accttgttgg acaagcacag gattgtcttt
    27781 cattgcatca aatgccggaa tcgtgcctgt ttcactgatg gtttgtgttt ctttcacatc
    27841 aaataacgac cagcgatgga atttcacccg atcgatatgc ggcaatggca gttcatctgc
    27901 tttcgctagt gcgtcttgac ggaaggttct catccaatca ggttcgcctt tacttaaaga
    27961 aaaatcattt accgcgtcaa gataatcgag ccagtttttt tctttcattc ttgctccccc
    28021 ttacgcttct tcttctttat agtcgattcc taattcttta ctgataccag cgtagccttc
    28081 tgcttccaga cgtttagcta ggtcagcatt ccctgtcatg actactcggc cttccatcat
    28141 gatgtgaacg acatcaggtg tgatatagtt caataatcgt tgatagtggg tgatgatcaa
    28201 tgcaccgaaa ttgtctccgc gcatttcatt tactccttta gcaacgactt tcaaagcatc
    28261 gatatccaga cctgaatcga tttcgtctaa aattgcaaat gtaggttcta acatcaacaa
    28321 ttgcaggatc tcattccgtt tcttttctcc gccagaaaaa ccttcgttca gataacgttc
    28381 agccatttct tctggcatat ttagtaaagc cattttttca tctaactttt tcaaaaatgc
    28441 cataacagaa attttgtcgt tttcatcacg tttcgcattg atcgctgcac gcatgaattc
    28501 cgcattcgtg atacctggta tctcacttgg atattgcata gctaaaaata agccaagtcg
    28561 cgcacgttca tctacttcca agtctaaaac atttttcccg tcaaacaaaa tttcgccttc
    28621 tgttacttca tagtttggat tgcccatgat agcggcagat aaggtagatt ttcctgtgcc
    28681 gttaggtccc atgatggcat ggatttctcc ggttttcatt gttaggttta cgcctttaag
    28741 gatttctttt tcctcgatcg acacatgtag attttttatt tctaagacag acatgcatta
    28801 tacccccaaa ttctctttcg ttctcactca ttttagacta attgagaatg aagcgcaatg
    28861 accttcctta ctaaaatcaa atatctttta tttattatta aacaattttg atttctaaat
    28921 tagaataggt ataaaaaaga tgagaaataa gccattacag acttatttct catctttttt
    28981 gttccatacc ttattttgca tcagccttta ctggtttaac agagccgccc catttgtctg
    29041 tgatccattc ttggacttct ttgcggtgaa gcacttcaat cagttttttg attttgtcac
    29101 tgtcttcatc acctttacga gtcgcaataa tattcacgta aggagagttg tccttttcaa
    29161 gcaatacggc atcttctaat ggattcaacc ctgcgccgta agcgaagttt gcgttgattg
    29221 caactaggtc accttcatca ttttggtacg tagaagctaa aagtgatggg tcgatcgtat
    29281 ggttgaactt caagttctta gggttttctt caatatcatc gaatgttgct gtttccagat
    29341 ctactccatc tttgactttg actaagccag catcttgtag aattgtgatg atgcgtcccc
    29401 agtctgattc agaatttgaa gtaatgattg ttgcaccatc ttttaattcg gaaacatctt
    29461 tgactttttt agagtacaag cccattggtt cgatgtggac agcaccggca ttgacaaagt
    29521 catatccttt ttctttgatt tctttgttca aataaggaat atgctggaag taattggcat
    29581 caatgtcgcc ttcttccaaa gattggttcg gtaatacata gtcatcgaat ttgacgattt
    29641 caagatcgat tccttcatct ttcaataatg gtttcacatg ttccaaaatc tctgcgtgcg
    29701 gtgtcggtga agcgccgact ttcaatgtcg tgtcgtctgc tttatttgct gaatctttgc
    29761 tgtcgctgtt gttaccgcat gctgctaatc ctacagtcag caaaagcgct gctgcaaatc
    29821 caaaaatttt ctttttcata tcctacatcc ccctatatcc tattatcttt tgtctgtttt
    29881 cttagttaag aaatcaccga ttccttgaac gataaatacg attagtaaaa taattaaagt
    29941 cgcaacgaat gtcactgtta gattaccacg ctggtagcct tcctgccaag caagacctcc
    30001 aagtccacct gcgccgatcg cgccggccat cgcagtaaat ccgatcatcg agatcgttgt
    30061 aactgttaat ccagaaatca atgcgggaat actttcaggt atcaagacct tccagatgat
    30121 ttcccagtaa cttgctccca ttgcatctgc tgcttccagt acaccagggc tgacttcacg
    30181 aaaagcaatc tctactagac gggcataaaa tggtgctgcg gataaaacaa gagctggaat
    30241 ggctgcttgc gcacctagca tagtgcctac gattgcttta gtaaatggaa tgatcaaaac
    30301 catcaggatc ataaatggcg ttgaccggaa aatattactg atgatcgata cgattccata
    30361 aatgattcga gcatacggtt tgtttttgcg tccaatagag taaaggatca atcccaaaac
    30421 taatccgatg atcgtcacta agaccattga gattaatgtc atatatagtg tatcaaggat
    30481 agcctgattc atcgtttccg ggttgatttg tttaaaatct agatatgttt ctgaaaagct
    30541 tttatccacg atggatcacc tcaattccta ctccttgttc tttgatgaac acttctgctt
    30601 gatctaattc tttggtttca ccagtcatca agacagtcaa tgaaccaaag gaaccttctt
    30661 tcacttgttt gatctttcca tacacaacgt tgacatcgat gttatagcgt cgaatcgctt
    30721 gggaaatgac tggctcgttg gcatttcttt cgctaaaggt caaagttgcc aaacggccgc
    30781 ttggattttc tttgatgagt tcagctaata gttctgtcga atcagcttct ggttcgatgt
    30841 cttgttgtac aaagcgtttg gttacttctt ttttcggttg tctgaagaca gtcaatacgt
    30901 ctccttcttc tacgactttc cccaactcca tcacagcgac tttgttgcag attttgcgga
    30961 tcacattcat ttcatgtgtg atcaagacga tggtcaaatt caatttctta ttgatcgcta
    31021 acaataagtc taatacttct tctgttgttt gcgggtccaa tgcgctagta gcctcatcac
    31081 aaagcaggat ctcaggatca tttgccaatg ctcgtgcgat accgacgcgt tgtttctgtc
    31141 cgccggaaag ttgtgaagga tatgcttgtc cgcgtccttc taacccgact aaacgcaaca
    31201 gttcttctgc ccgttcttta cgtttgctct ttggtacgcc agctaattcc agcggcagct
    31261 ggatattttc aagtacagtt cttgaccata acaggttgaa atgctggaag atcataccga
    31321 ttttctttcg gaatgtacgt aattctttgt tttttaattt tgtaatatct tgatcgttta
    31381 cgatcacttc gccgctcgtg ggaagctcaa gtccattcag gagacggact aatgtacttt
    31441 ttcctgcgcc agaatagccg acgattccat aaatatcccc tttttcaacg tttagtgaaa
    31501 cgtcattgac ggcttggatg tttccgtttt tcccattgaa tgtctttttg acattcgtta
    31561 gttggattaa tgacatgcca ttcacatctt tctcttataa tttttcaaac aaaaaagcct
    31621 tcgccctctt gccttaagca aaaggacgaa agcttgaact tccgtgttac caccttgatt
    31681 taccgttact tcgcaataac agcctcttcc agtacttgga cttagcttct ttccgtatac
    31741 tgtcgcgctg taacgggcac tcccgtagta agtttgctta cgctcgctta ctctgctcta
    31801 agaccatctt ccatctcatt ttcgttaccc attttcagca aacagggctc tcttcaaacg
    31861 tccgtgacat gtactcttct tttcaaagct ttctcgtttc atataggatt gcttatgatt
    31921 atagcaactg gaagaatcag tgtcaacttt tttcaagtga ttttctgatt ttataattcg
    31981 tcaaattctt ttggatcaac atctttgaaa cttaagatcc acgcattcat ttcatctgaa
    32041 tcattcaaga tattgatatc ttcaccgatt ttatcattaa cagaagaaac tgtacccgtt
    32101 agcgggctgg caaattcatt gaccgctttt tccgcttcaa cttcgatcag aggttcatct
    32161 ttttttagcg attgtcctgc ttttggcaga ttggcaaacg tgatgtctcc aagttcttct
    32221 tgcgcttcat ttgtcaaacc aacacagtat tcttttccat taaataagat ccaaaggttg
    32281 tcctttttct tcaaatgctt gcctgccatt ttatatcagt tctccttcat aaattttttc
    32341 attgaatcca attgcaacaa gctggtcctc tcgtaagatc agtggtcgct tgatcagcat
    32401 accatctgtc gataagactt ggctcgcttt tgaagccgta aactcgccta cttgatcttt
    32461 cagtccaagt tcccgatatt tcatcccgct tgtgttgaac aggcgacgga ttggtacttg
    32521 actcgtttct agccaagtct caatcgttga ggccttcggt acttctttga tcatatcaac
    32581 ggtttgataa ctaacgccat gctggtctaa ccacgctttg gcttttttgc atgttgaaca
    32641 tttcgggtac cagtaaaagg tgtacaattt tcttacctcc ttgaagatct tgcaggtgct
    32701 tcattttggt atctcatcga taatatcaac ccgataccga tcatgttccc taaaatcgag
    32761 gatcctccct gactaatgaa aggcaatggg atccctgtta aaggaagtaa tccgatattt
    32821 gctccaatat tctcaaagac atggaacagc agcatcatga tcaaaccgct tgcgatatag
    32881 gcgtaaaatt cattattcgt atcgaaacac acccgaatca tacgatagat taaaataaaa
    32941 tacaagaaaa tgacgaatgc accaccgata aagccgaagt tttcaccgat aacggtaaaa
    33001 atcatatcgg actcacgaac aggcacatag atattgctta cgttgaatcc tttaccaaac
    33061 aagccaccgg taccaattgc caaaagtcct tgggccggct gataactcat tcctgacgta
    33121 tcatggaaag ggtccagcca tgaatcaata cgtgcaaact gataagattt gaatccaact
    33181 ttataaagca agtctcttcc gccttcagtc gtaaccaaga agatcgttcc tgctccaacg
    33241 actactgcca agattactac aggtgcgatg atgcgccaag agattccaga catcaggaaa
    33301 atcccgccaa agatcgcaag aaagacaagc atcgttccaa agtcatcttg ggccatgatc
    33361 aatgctaaaa ccggtattgt cacgatcaag agcttcccaa tcagccaaaa gtccgatttc
    33421 atcgtccgtt cttttacttg cgtattatgt ttagttacga tcagtgccat catcaagatg
    33481 tacgcaatct tcatcaactc tgctggctgg aaagtaaacg caccgaatcg aaaccagttt
    33541 ttagaccctg ttgaagcagc taaattctga tcatagaatt tgagcaagag tcccattacc
    33601 actaacccca aaccataaag gtaaggagtc agtttccaga tccatttgga attcaaatgc
    33661 atgatgacaa cgattgctgc cgctccaact aaataccaga caacttgcat ccctacgcct
    33721 ttcaagactg ctggattgtt cggatcatgg ctaagtgcta catataatga gagcagacca
    33781 atcaaacata acagaaagac tggtaaaatc acgccataat caatacgatt atcgttcgaa
    33841 gcttttaatt ttttttccat gtttcatccc ttttcttaaa aaccatactg ctgattatta
    33901 tagcggtcca gttagataat ggaaagtctt tccatagaca tttgggaaaa attaactttc
    33961 cgtttgtgca gttgcttgag caaatgcagt acaaaagcct actgcttatc tttagtgtat
    34021 catagaactt tttttgatac atgcaattat ctaccagctg agattctcct cgctgtgaga
    34081 agtttctgtg ctttcattgt atcgaaggat ctcttcggca atctctagca gctgatcaga
    34141 aaccagattg gttcgttgga tgaaataaaa atatcgatgg aaattcaacg gagtcattgg
    34201 cacattttct ggaacagagc gcttagacaa aatcgagcga ccgattcctt ccttcagcaa
    34261 cgcaacaatt acatcgttac ttttcacgat catcttctta tcttggatat tcatctcttc
    34321 aaaataacgc tgcgtgtaat gaaaaacacc agagtttgct tctcttacaa gccacaattt
    34381 gctttttggg tccccagcga tgactagctg gtcttccatc aaagagtatc tttgtacgcc
    34441 ggaagtctct aatggctttt cgataaaacc aaaatcggat tcatgtttcg ctacttgttc
    34501 taatgattca tgagaattcg tgatttcgat ctcaaaatgc actttaggaa accttttgat
    34561 cagatgtttc atcaactgag gcaaaagata gacagcaaag gtgtgggaag caacgatccg
    34621 acatacttct tcttcatttt gttcattgag caatttttgc tgagtttctt cccattcttc
    34681 taacaaaacc agtgtccgtt catataataa gtcggcttgc ttagttggaa gcatctcttt
    34741 tcttccgtta cgttgaaaaa gatcgatatt caattcccgc tccaactgtt tgatttgatt
    34801 cgagactgct ggttgagata aaaacagtac ttctgctgct ctggaaaaat ttcttgtttc
    34861 atatactgct cgaaaagtcg ttaataactt aaacatggca ccacttccat aactttttat
    34921 gataattact ataaaaacta tttgatttaa atatacaaaa aaccatctat aatagaaatg
    34981 tacaacaaaa cagttgctga tccaactagt aacttagatg aagacaatca gctgtctgct
    35041 tgctcgtaat catacattcc tattcatttt acaatgtatt cgttcatctt ggtactaaaa
    35101 tctattaaat aaaaaggaga atattatata tgaagaactt tttgaaaatt attccgatcc
    35161 cgatctgcgg gttgatcctt gggttagctt cccttggaaa tctgtttaaa gactatcacc
    35221 atgttgcttt aggcaatcta actggcggta tctctatgat tcttatgata ttcatttttg
    35281 ttaaattgtt tatcttattc gaacacacga aacaaacgtt gcatgatcca atcatcgcct
    35341 ccgtttcacc aacttttacg atgtcactca tggttatttg tacttatttt gtttcatatg
    35401 aaagtattgc accaattgtc aaattcattt ggctgtgcgc tgttatcttc caagtgatcc
    35461 ttgtctttta tttcaattat caccatgtta tcaaagctga tctttcaatt gaagcaattt
    35521 atccaagctg gtttatcatt tacgttggat ttggggtcat cactgtaact gccgggaact
    35581 tcttcccact tattggaaaa attttctttt ggatttcatt gatctgctat gcgttcttat
    35641 tgccgatcat catcaaacgt gtctttttcg taagaaatat ggctcatccg acacgtccgc
    35701 tgattacgat cgttgcagca cctggttctc tttgcctaac aggttatctt aaaaactttg
    35761 ctgaacctaa tttttatctt gtcgtagcat tgttcgcttt atctcaagta ttgtatttcg
    35821 ttgtgctgtc tatgcttcca cgtttgttca agcttcgttt ttatccaagc tatgcagcct
    35881 ttacattccc gcttgttatc tctgcaacag ctatatttac tactgttcat tacttcaaca
    35941 gtttaggtat ctatagtagc gtattggatt tcttaaaagt tgcggaatgt ctgtttgcaa
    36001 gtgtagttgt cttctacgtt cttgctcatt acgtacgttt cttaattaaa gaacatagca
    36061 aacatggaaa atttgtttct aaaaaaatcg cttaataatc gattcaatag gttgtgaaaa
    36121 agctgttctg catcaagtaa taagaacggc caaacaaaaa cagctgttca tgtttttgta
    36181 ctggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggtcgtaa
//
LOCUS       DACCCS010000025        35882 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.025, whole genome shotgun sequence.
ACCESSION   DACCCS010000025 DACCCS010000000
VERSION     DACCCS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.025"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1152
                     /locus_tag="IVK82_001280"
     CDS             <1..1152
                     /locus_tag="IVK82_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392353.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IVK82_001281"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IVK82_001281"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP6392354.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IVK82_001282"
     CDS             2728..3003
                     /locus_tag="IVK82_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP6392355.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IVK82_001283"
     CDS             3009..3776
                     /locus_tag="IVK82_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP6392356.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IVK82_001284"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IVK82_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP6392357.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IVK82_001285"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IVK82_001285"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP6392358.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IVK82_001286"
     CDS             7920..8222
                     /locus_tag="IVK82_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP6392359.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IVK82_001287"
     CDS             8225..9388
                     /locus_tag="IVK82_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP6392360.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IVK82_001288"
     CDS             9414..12842
                     /locus_tag="IVK82_001288"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP6392361.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IVK82_001289"
     CDS             12863..13987
                     /locus_tag="IVK82_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392362.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IVK82_001290"
     CDS             13977..14414
                     /locus_tag="IVK82_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP6392363.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IVK82_001291"
     CDS             14398..14736
                     /locus_tag="IVK82_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP6392364.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IVK82_001292"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IVK82_001292"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP6392365.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IVK82_001293"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IVK82_001293"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP6392366.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IVK82_001294"
     CDS             15893..16936
                     /locus_tag="IVK82_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP6392367.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IVK82_001295"
     CDS             17095..17823
                     /locus_tag="IVK82_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP6392368.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IVK82_001296"
     CDS             17939..18184
                     /locus_tag="IVK82_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6392369.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IVK82_001297"
     CDS             18181..18399
                     /locus_tag="IVK82_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP6392370.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IVK82_001298"
     CDS             18535..19074
                     /locus_tag="IVK82_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP6392371.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IVK82_001299"
     CDS             19067..21340
                     /locus_tag="IVK82_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP6392372.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IVK82_001300"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IVK82_001300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP6392373.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IVK82_001301"
     CDS             complement(22513..22767)
                     /locus_tag="IVK82_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP6392374.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IVK82_001302"
     CDS             complement(23167..23436)
                     /locus_tag="IVK82_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392375.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="IVK82_001303"
     CDS             complement(23906..24361)
                     /locus_tag="IVK82_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP6392376.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="IVK82_001304"
     CDS             complement(24499..26427)
                     /locus_tag="IVK82_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP6392377.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="IVK82_001305"
     CDS             complement(26624..27457)
                     /locus_tag="IVK82_001305"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP6392378.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="IVK82_001306"
     CDS             complement(27649..28353)
                     /locus_tag="IVK82_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6392379.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="IVK82_001307"
     CDS             complement(28376..29002)
                     /locus_tag="IVK82_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6392380.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="IVK82_001308"
     CDS             29328..31703
                     /locus_tag="IVK82_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP6392381.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="IVK82_001309"
     CDS             complement(31727..32218)
                     /locus_tag="IVK82_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP6392382.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="IVK82_001310"
     CDS             32405..33325
                     /locus_tag="IVK82_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6392383.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="IVK82_001311"
     CDS             33479..33823
                     /locus_tag="IVK82_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP6392384.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="IVK82_001312"
     CDS             complement(34013..34513)
                     /locus_tag="IVK82_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6392385.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="IVK82_001313"
     tRNA            35041..35124
                     /locus_tag="IVK82_001313"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
     gene            complement(35448..>35882)
                     /locus_tag="IVK82_001314"
     CDS             complement(35448..>35882)
                     /locus_tag="IVK82_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP6392386.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11869 a         6261 c         7404 g        10348 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgttg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta gtaccaaccg
    35461 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    35521 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    35581 tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    35641 gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc tgttacagca
    35701 ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac cgtttcttgt
    35761 acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg aacggctgct
    35821 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    35881 ta
//
LOCUS       DACCCS010000026        34789 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.026, whole genome shotgun sequence.
ACCESSION   DACCCS010000026 DACCCS010000000
VERSION     DACCCS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.026"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(233..1081)
                     /locus_tag="IVK82_001315"
     CDS             complement(233..1081)
                     /locus_tag="IVK82_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6392387.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IVK82_001316"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IVK82_001316"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP6392388.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="IVK82_001317"
     CDS             complement(3582..3761)
                     /locus_tag="IVK82_001317"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP6392389.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IVK82_001318"
     CDS             complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IVK82_001318"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP6392390.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /locus_tag="IVK82_001319"
     CDS             5909..8188
                     /locus_tag="IVK82_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6392391.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="IVK82_001320"
     CDS             complement(8281..8928)
                     /locus_tag="IVK82_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392392.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="IVK82_001321"
     CDS             complement(9107..9826)
                     /locus_tag="IVK82_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6392393.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="IVK82_001322"
     CDS             complement(9957..11108)
                     /locus_tag="IVK82_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP6392394.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="IVK82_001323"
     CDS             complement(11325..12065)
                     /locus_tag="IVK82_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6392395.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IVK82_001324"
     CDS             complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IVK82_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP6392396.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12449..13042)
                     /locus_tag="IVK82_001325"
     CDS             complement(12449..13042)
                     /locus_tag="IVK82_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6392397.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13673)
                     /locus_tag="IVK82_001326"
     CDS             complement(13026..13673)
                     /locus_tag="IVK82_001326"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP6392398.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IVK82_001327"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IVK82_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP6392399.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IVK82_001328"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IVK82_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP6392400.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="IVK82_001329"
     CDS             complement(15119..15646)
                     /locus_tag="IVK82_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP6392401.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15826..16611)
                     /locus_tag="IVK82_001330"
     CDS             complement(15826..16611)
                     /locus_tag="IVK82_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP6392402.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16604..17473)
                     /locus_tag="IVK82_001331"
     CDS             complement(16604..17473)
                     /locus_tag="IVK82_001331"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP6392403.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17474..18853)
                     /locus_tag="IVK82_001332"
     CDS             complement(17474..18853)
                     /locus_tag="IVK82_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP6392404.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IVK82_001333"
     CDS             complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IVK82_001333"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP6392405.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IVK82_001334"
     CDS             complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IVK82_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP6392406.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IVK82_001335"
     CDS             complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IVK82_001335"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP6392407.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IVK82_001336"
     CDS             complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IVK82_001336"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP6392408.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IVK82_001337"
     CDS             complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IVK82_001337"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP6392409.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22811..23035)
                     /locus_tag="IVK82_001338"
     CDS             complement(22811..23035)
                     /locus_tag="IVK82_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP6392410.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23088..24050)
                     /locus_tag="IVK82_001339"
     CDS             complement(23088..24050)
                     /locus_tag="IVK82_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP6392411.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24060..24500)
                     /locus_tag="IVK82_001340"
     CDS             complement(24060..24500)
                     /locus_tag="IVK82_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6392412.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24806..25771
                     /locus_tag="IVK82_001341"
     CDS             24806..25771
                     /locus_tag="IVK82_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP6392413.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25828..26058)
                     /locus_tag="IVK82_001342"
     CDS             complement(25828..26058)
                     /locus_tag="IVK82_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP6392414.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26144..27031)
                     /locus_tag="IVK82_001343"
     CDS             complement(26144..27031)
                     /locus_tag="IVK82_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP6392415.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27120..28343)
                     /locus_tag="IVK82_001344"
     CDS             complement(27120..28343)
                     /locus_tag="IVK82_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6392416.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28360..29439)
                     /locus_tag="IVK82_001345"
     CDS             complement(28360..29439)
                     /locus_tag="IVK82_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6392417.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IVK82_001346"
     CDS             complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IVK82_001346"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP6392418.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31297..31563)
                     /locus_tag="IVK82_001347"
     CDS             complement(31297..31563)
                     /locus_tag="IVK82_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP6392419.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32042..34276
                     /locus_tag="IVK82_001348"
     CDS             32042..34276
                     /locus_tag="IVK82_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP6392420.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34424..34744
                     /locus_tag="IVK82_001349"
     CDS             34424..34744
                     /locus_tag="IVK82_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP6392421.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9843 a         7509 c         6064 g        11373 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15841 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15901 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15961 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    16021 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    16081 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    16141 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16201 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16261 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16321 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16381 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16441 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16501 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16561 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16621 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16681 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16741 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16801 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16861 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16921 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16981 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    17041 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    17101 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    17161 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17221 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17281 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17341 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17401 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17461 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17521 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17581 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17641 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17701 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17761 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17821 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17881 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17941 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    18001 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    18061 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    18121 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18181 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18241 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18301 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18361 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18421 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18481 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18541 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18601 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18661 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18721 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18781 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18841 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18901 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18961 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    19021 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    19081 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    19141 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19201 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19261 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19321 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19381 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19441 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19501 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19561 tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac attgactggc
    19621 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19681 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19741 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19801 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19861 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19921 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19981 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    20041 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    20101 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    20161 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20221 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20281 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20341 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20401 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20461 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20521 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20581 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20641 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20701 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20761 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20821 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20881 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20941 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    21001 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    21061 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    21121 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21181 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21241 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21301 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21361 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21421 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21481 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21541 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21601 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21661 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21721 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21781 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21841 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21901 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21961 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    22021 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    22081 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    22141 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22201 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22261 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22321 cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa
    22381 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22441 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22501 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22561 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22621 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22681 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22741 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22801 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22861 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22921 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22981 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    23041 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    23101 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    23161 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23221 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23281 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23341 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23401 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23461 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23521 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23581 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23641 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23701 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23761 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23821 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23881 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23941 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    24001 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    24061 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    24121 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24181 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24241 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24301 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24361 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24421 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24481 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24541 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24601 tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa
    24661 tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg
    24721 aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt
    24781 tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct
    24841 gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa
    24901 acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt
    24961 attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga
    25021 tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat
    25081 gaattatttc agtaattata attatgtaca tattaccaat aagggatttg tattaacaga
    25141 tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt
    25201 gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt
    25261 gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aggaactcca
    25321 agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa gacaacaaca
    25381 tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata tcgaattagt
    25441 tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac catcgctcac
    25501 cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg tttgttatgg
    25561 tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct attatgaaaa
    25621 gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca
    25681 aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg aactgttaaa
    25741 caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt aggcatcttc
    25801 aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt ccatcgctac
    25861 gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta attcaaatgt
    25921 atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt caaaacggcg
    25981 aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta tgattgcgac
    26041 taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa
    26101 gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat tttttttctg
    26161 gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca ttctcaaaac
    26221 ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc aatacaagat
    26281 ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa
    26341 aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa gaccagttag
    26401 ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg tttttttgtg
    26461 catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc tgattagcca
    26521 tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta ccaaattctt
    26581 tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg atcgtcagtg
    26641 taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca tctgcgcctt
    26701 tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta gtcaaatcaa
    26761 ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag tagatttctt
    26821 ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct gctaccgctt
    26881 ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt gcttttgtcc
    26941 gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca
    27001 tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc
    27061 ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat gtttgaattt
    27121 tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc ttcttctttt
    27181 cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat catagcttcg
    27241 ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg atctttagga
    27301 acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt
    27361 ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc cacgacacaa
    27421 ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga agtagaagca
    27481 ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc cacttctcct
    27541 gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa ctcttctaca
    27601 taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat gacctgagga
    27661 aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa agaaagaatc
    27721 atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc ctgctgagtg
    27781 atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct
    27841 attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac tacacctgtc
    27901 gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat cacttttcct
    27961 ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat cagaggtatt
    28021 ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt atgggtatga
    28081 ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc tctaacaaca
    28141 actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac atctttttcc
    28201 aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc atgtctttct
    28261 aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg aaagtaggta
    28321 tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga cctgatttac
    28381 tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat ttttaagttc
    28441 ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt gtgtcgtact
    28501 cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt ttgtcttccc
    28561 catatagcag ttcttgctat cttctaacca gccattatac actggaatat ctcgaacaag
    28621 tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct cttcataaga
    28681 agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc cacgaatgta
    28741 accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga tgttccgagg
    28801 aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca tcttcttagc
    28861 aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac aaatgattac
    28921 tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca ttggatcagg
    28981 gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac ggataccata
    29041 gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg ctttttggta
    29101 caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatccga taaaggaatt
    29161 tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt ctcttctggc
    29221 tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga tgtcataatc
    29281 ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt tttgatgatc
    29341 aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat ataataatac
    29401 cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac tgtcactcaa
    29461 acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa aaagaacaaa
    29521 aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt tattttacat
    29581 attcatgaac aagttcaacg acttcttcca tcgtatcgca ttctttcaat gcacgatcag
    29641 caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt ttaagaatag
    29701 atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga acagctgttt
    29761 gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt gctgcatcaa
    29821 ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag taagaaacgc
    29881 gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta cctacagaga
    29941 agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga atctcgatca
    30001 tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat ttttgttttt
    30061 cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc atagggaaca
    30121 tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt gtacggaaca
    30181 tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag aatggattca
    30241 tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc attgtacgaa
    30301 caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca tattggtcat
    30361 cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac agaccaactg
    30421 cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg tttgcagcta
    30481 attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt tcccattctg
    30541 ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt tcatctgtag
    30601 gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct tctttgactt
    30661 tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt gccatgatag
    30721 cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta
    30781 attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct tcgttgatca
    30841 ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta gcaacgtcac
    30901 gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg aacataccga
    30961 tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg tctttaatat
    31021 tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg gcatcaaata
    31081 cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc tgcaattctt
    31141 cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta tcttctactg
    31201 ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca acggctacac
    31261 cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc cttctttctt
    31321 cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg cagtaatagt
    31381 aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt tcaagtttac
    31441 tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg tttgtactaa
    31501 tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt ctttcttttc
    31561 catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt cttttttagt
    31621 aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca ttttttttcg
    31681 ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt ccggattctt
    31741 tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc ttgtcgcttt
    31801 ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat atgaaacgaa
    31861 ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga atggtcaata
    31921 gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat tcttttacct
    31981 catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac gtgaatgatt
    32041 catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa
    32101 tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca aaaatcaaca
    32161 aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca gtttggacga
    32221 tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc aaacaccacc
    32281 aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag gcaatacaga
    32341 aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag gtgatatcga
    32401 tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa atcgccgaac
    32461 caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag ttgttgaagg
    32521 tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca aagaagtcat
    32581 tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat ttgaagaacg
    32641 tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat tcatagatga
    32701 agttcacgaa atagtcggtg ctggttctgc tggtgatgga aatatggatg ctggtaatat
    32761 tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta ccttaaatga
    32821 gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg tacgggtaga
    32881 tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga
    32941 ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt tatccaatcg
    33001 ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg aaacaggttc
    33061 taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga
    33121 agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag cagcttatta
    33181 tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac
    33241 acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag gtattccagt
    33301 gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg acttgaaagc
    33361 tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg
    33421 tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg gaccaactgg
    33481 tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt cacaagattc
    33541 aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta aattgattgg
    33601 ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa aagtacggag
    33661 aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag atgttttgca
    33721 tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa gaacggtcag
    33781 ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag tagaagcaaa
    33841 cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc aattaaacaa
    33901 ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca gtgcattatc
    33961 aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc aaatgctggc
    34021 agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag ttgacttagg
    34081 ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga
    34141 tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag caaaactgga
    34201 taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag aatcagccga
    34261 agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg agaaatgccg
    34321 tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag aaattataat
    34381 aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat tggtaaatgt
    34441 aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg cccatctagt
    34501 caaggtctac acagccggaa ataccactat tgtctacact gaagcccctg aacataatga
    34561 aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa
    34621 ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca aagtaatcga
    34681 attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta cattagccgt
    34741 gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaagtaaa
//
LOCUS       DACCCS010000027        34687 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.027, whole genome shotgun sequence.
ACCESSION   DACCCS010000027 DACCCS010000000
VERSION     DACCCS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.027"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            591..1280
                     /locus_tag="IVK82_001350"
     CDS             591..1280
                     /locus_tag="IVK82_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6392422.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IVK82_001351"
     CDS             1267..2475
                     /locus_tag="IVK82_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6392423.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIVSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IVK82_001352"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IVK82_001352"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP6392424.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IVK82_001353"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IVK82_001353"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP6392425.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IVK82_001354"
     CDS             complement(6578..7273)
                     /locus_tag="IVK82_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP6392426.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IVK82_001355"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IVK82_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP6392427.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IVK82_001356"
     CDS             complement(8472..8663)
                     /locus_tag="IVK82_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP6392428.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IVK82_001357"
     CDS             complement(8684..9574)
                     /locus_tag="IVK82_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP6392429.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IVK82_001358"
     CDS             complement(9561..10328)
                     /locus_tag="IVK82_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6392430.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IVK82_001359"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IVK82_001359"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP6392431.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IVK82_001360"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IVK82_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP6392432.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IVK82_001361"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IVK82_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP6392433.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IVK82_001362"
     CDS             14944..15282
                     /locus_tag="IVK82_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392434.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IVK82_001363"
     CDS             complement(15450..16208)
                     /locus_tag="IVK82_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP6392435.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IVK82_001364"
     CDS             complement(16226..17044)
                     /locus_tag="IVK82_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP6392436.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IVK82_001365"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IVK82_001365"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP6392437.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IVK82_001366"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IVK82_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP6392438.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IVK82_001367"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IVK82_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP6392439.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IVK82_001368"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IVK82_001368"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP6392440.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IVK82_001369"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IVK82_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP6392441.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IVK82_001370"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IVK82_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP6392442.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IVK82_001371"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IVK82_001371"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP6392443.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IVK82_001372"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IVK82_001372"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP6392444.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IVK82_001373"
     CDS             27086..27385
                     /locus_tag="IVK82_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP6392445.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IVK82_001374"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IVK82_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6392446.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IVK82_001375"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IVK82_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP6392447.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IVK82_001376"
     CDS             28352..30325
                     /locus_tag="IVK82_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP6392448.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IVK82_001377"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IVK82_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP6392449.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IVK82_001378"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IVK82_001378"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP6392450.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="IVK82_001379"
     CDS             33204..34496
                     /locus_tag="IVK82_001379"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP6392451.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10568 a         6739 c         6857 g        10523 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcgtttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt
    34681 ttccgaa
//
LOCUS       DACCCS010000028        33295 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.028, whole genome shotgun sequence.
ACCESSION   DACCCS010000028 DACCCS010000000
VERSION     DACCCS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.028"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..112
                     /locus_tag="IVK82_001380"
     CDS             <1..112
                     /locus_tag="IVK82_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP6392452.1"
                     /translation="VLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            140..775
                     /locus_tag="IVK82_001381"
     CDS             140..775
                     /locus_tag="IVK82_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP6392453.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            795..1568
                     /locus_tag="IVK82_001382"
     CDS             795..1568
                     /locus_tag="IVK82_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6392454.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            1976..2773
                     /locus_tag="IVK82_001383"
     CDS             1976..2773
                     /locus_tag="IVK82_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP6392455.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            2751..3164
                     /locus_tag="IVK82_001384"
     CDS             2751..3164
                     /locus_tag="IVK82_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392456.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            3148..5793
                     /gene="mutS"
                     /locus_tag="IVK82_001385"
     CDS             3148..5793
                     /gene="mutS"
                     /locus_tag="IVK82_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP6392457.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            5811..7919
                     /gene="mutL"
                     /locus_tag="IVK82_001386"
     CDS             5811..7919
                     /gene="mutL"
                     /locus_tag="IVK82_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP6392458.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            7941..8501
                     /locus_tag="IVK82_001387"
     CDS             7941..8501
                     /locus_tag="IVK82_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP6392459.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(8586..10574)
                     /gene="treP"
                     /locus_tag="IVK82_001388"
     CDS             complement(8586..10574)
                     /gene="treP"
                     /locus_tag="IVK82_001388"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP6392460.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            10797..11510
                     /gene="treR"
                     /locus_tag="IVK82_001389"
     CDS             10797..11510
                     /gene="treR"
                     /locus_tag="IVK82_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP6392461.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            11587..12033
                     /gene="msrB"
                     /locus_tag="IVK82_001390"
     CDS             11587..12033
                     /gene="msrB"
                     /locus_tag="IVK82_001390"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP6392462.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            12203..12805
                     /gene="ruvA"
                     /locus_tag="IVK82_001391"
     CDS             12203..12805
                     /gene="ruvA"
                     /locus_tag="IVK82_001391"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP6392463.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            12818..13819
                     /gene="ruvB"
                     /locus_tag="IVK82_001392"
     CDS             12818..13819
                     /gene="ruvB"
                     /locus_tag="IVK82_001392"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP6392464.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            13848..14426
                     /locus_tag="IVK82_001393"
     CDS             13848..14426
                     /locus_tag="IVK82_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP6392465.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            14478..14960
                     /locus_tag="IVK82_001394"
     CDS             14478..14960
                     /locus_tag="IVK82_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP6392466.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            15077..15451
                     /locus_tag="IVK82_001395"
     CDS             15077..15451
                     /locus_tag="IVK82_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392467.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(16129..17481)
                     /gene="brnQ"
                     /locus_tag="IVK82_001396"
     CDS             complement(16129..17481)
                     /gene="brnQ"
                     /locus_tag="IVK82_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP6392468.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            17628..18218
                     /locus_tag="IVK82_001397"
     CDS             17628..18218
                     /locus_tag="IVK82_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP6392469.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(18346..19695)
                     /locus_tag="IVK82_001398"
     CDS             complement(18346..19695)
                     /locus_tag="IVK82_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6392470.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(19863..20603)
                     /locus_tag="IVK82_001399"
     CDS             complement(19863..20603)
                     /locus_tag="IVK82_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6392471.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(20616..22208)
                     /locus_tag="IVK82_001400"
     CDS             complement(20616..22208)
                     /locus_tag="IVK82_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6392472.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(22776..23072)
                     /locus_tag="IVK82_001401"
     CDS             complement(22776..23072)
                     /locus_tag="IVK82_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392473.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            23248..23973
                     /locus_tag="IVK82_001402"
     CDS             23248..23973
                     /locus_tag="IVK82_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP6392474.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            23966..24808
                     /locus_tag="IVK82_001403"
     CDS             23966..24808
                     /locus_tag="IVK82_001403"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP6392475.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            24811..25332
                     /locus_tag="IVK82_001404"
     CDS             24811..25332
                     /locus_tag="IVK82_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP6392476.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(25585..26148)
                     /locus_tag="IVK82_001405"
     CDS             complement(25585..26148)
                     /locus_tag="IVK82_001405"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP6392477.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            26399..26668
                     /gene="rpsO"
                     /locus_tag="IVK82_001406"
     CDS             26399..26668
                     /gene="rpsO"
                     /locus_tag="IVK82_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP6392478.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            26856..28982
                     /gene="pnp"
                     /locus_tag="IVK82_001407"
     CDS             26856..28982
                     /gene="pnp"
                     /locus_tag="IVK82_001407"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP6392479.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            29492..29758
                     /locus_tag="IVK82_001408"
     CDS             29492..29758
                     /locus_tag="IVK82_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6392480.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            29902..31893
                     /locus_tag="IVK82_001409"
     CDS             29902..31893
                     /locus_tag="IVK82_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP6392481.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            32247..>33295
                     /locus_tag="IVK82_001410"
     CDS             32247..>33295
                     /locus_tag="IVK82_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP6392482.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIA"
BASE COUNT        10887 a         6177 c         7028 g         9203 t
ORIGIN      
        1 tgtgttacat aatttgtttt ttgctttggc aggaaattat attggtggcg gtttagtaat
       61 gggactggga tatgcttggc tgaatcagtc aaaatcatcc tatgtggact aaaaagtcgt
      121 tcattaaagg ggaagtgcca tggaagtttt agtttttcca aaaaatgagg aaaaagcgtt
      181 acaaatggaa aagtacatga aaaatctttt ttcctttgct ggagtaccta agccggaacg
      241 cgctctttta caacaaccgt tactggatga gagcaaaaat ttatctattt ctgaagtttt
      301 agcttttgtt cattattatt atgaacaaat cgaaagagag tatcagtatg tagcgatcga
      361 tttagtccag aaaaacatca aacggcttac atttgaagac ttagtcgtat tgttgccact
      421 agttcagaaa aaagaatggt gggacagcat cgactcttgg agaaaagtat acagcacatg
      481 gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa ttgtttgaaa aaaaggaaga
      541 tttttggcta cgtcgaatag caatcacgtt gcaattaggt tataaacagc aaacagacac
      601 aggactattg atccgagcaa tagacgaaga tcgtcaaaca aaggaattct ttatccaaaa
      661 agcaattggt tgggcattac gcgattatag taagaccgat gccgattggg taaaaagaca
      721 gctgacaaca gatttgagta agttagctgt tcgagaggca agtaagtatt tataagaaag
      781 cgagacgaca agagatgaga cgcaaattat ttgcttttga tattgatgga acgttgttag
      841 attcaaacaa acaagcttta gatagcacaa gagaagcatt ggaaaaatta cgcaaagccg
      901 gtcatctggt cacgattgct acagggcgga gccgatttca tgcgcaagag atcattcgcg
      961 atcttgattt tacgaactac attttatgta atggagcggc cggtttttta gaccatgaac
     1021 aagtatataa gaatttacta gatcgcgacc agttggatgc gttcgttcaa gaagcgcatc
     1081 atgagcagat cgatacagct tttgtcagct tagacaatat caagcgttct tcttccaatg
     1141 atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc tgtcttgcct gaattagatc
     1201 agttttttgc agaggaacaa gaaatatacc aagcgttagc atttttcgat caaacatatg
     1261 atgggaagtt ctcttcatat gataaaattc gatttgtccg ctggcatgag aacagtgtag
     1321 atgtcgtacc taaaaatggt tctaaagcag cgacaattct gcacttagca gaacaagtag
     1381 gcatccctca tgaagacatc atcagctttg gcgatggaca aaacgaccgt gaaatgttgc
     1441 gcatgtcagg aattgggata gctatgggga atgctgtgcc agaagtacaa gcagaagcta
     1501 agatggtcac tgatacaaac gatcaggatg gaatttggaa ggcattgaaa gagttaagcg
     1561 tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag taataagccc agccaaacaa
     1621 aaatagctcc tcctattttt cgtttttctg ggcttattac cgcagatgca agctttcgaa
     1681 caccgtttaa ggaagaggtc gtgacagaag tggtcagttt caagaaataa gaagggattt
     1741 acgaaaattg cttctcaaat ttttgtaaaa ttacggctta tttctgaaga agtagatgaa
     1801 acaacgttta ttcgggaaat gggactgtga caagatgttt gtcatagtcc cattttttta
     1861 ttttattttt caaaactaag aaataccagg aaagagttcc ttttgtttga ccttttcttt
     1921 cttattgaaa acaactataa tatagagtga tgttaaaaga gaagaggaat ttagtgtgcg
     1981 ggtattgttt attggtgatg tagtaggttc aatgggacga gagatgatta ctgaatatct
     2041 tcctagattg aagaagaagt accgtccgca agtaacgatt gtaaatggtg agaatgccgc
     2101 ttctggacgt ggaatcacag aaaaaatcta taaaaaattt ttgcaagacg gcgtagatgt
     2161 cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc tttgagtttg taaatgaagc
     2221 taaaaagatg gttcgtccgg caaattttcc ggaagggacg cctggacaag ggatggtctt
     2281 tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg caggctcgtt cttttatggt
     2341 ggacttggat gatccttttc gtaaaatgaa tgagctggta gaagaagcca gaaaacgtac
     2401 gccgatcatt tttgtagatt ttcatggaga aacaacaagt gaaaaacaag caatgggctg
     2461 gtttttagat gggaaagttt cagcagtggt cggtacacat acacacgttc agacaaatga
     2521 tgcgcgaatt ttgccaagag gaacagcgta tctgacagac gttgggatga caggtcccta
     2581 tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa aagtttctga ctgcattacc
     2641 aaaacgtttt gaagttgtgg aacaaggccg atcgatttta agcggctgta tcttggaatt
     2701 ggatgatacg acaggtcatg caaaagaaat ccagctgatt cagatcaacg atgatcgacc
     2761 gttcatggag tagaaagaga ggcagttttt tgcagcagga gccggaaatt caaaaagaag
     2821 taaggaaact gacaaaactt ttaagagaga atgaaacgat cattcgctac aaagaacttg
     2881 aagaaaagat ccagcaaaac cagtatttag ctgagttgag agaaaaaatc aaacaagcgc
     2941 aaaaagatgc tgttcatttt gcccactatg acaaaccagc agcagaaaaa gaagcaatca
     3001 aacaagcgga tcaattcatg caagaatttg atcagcatcc gcttgttgta gcttatagaa
     3061 aacagctgtt ggaagcagat gatctgcttc atcatttaac aactatgata caagaagaaa
     3121 tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa ctaaaaatac tcctatgatg
     3181 gaacaatatc taagcatcaa ggcccaatac caagatgctt ttttgttcta tcgtttagga
     3241 gacttttatg aactatttta cgaagatgcg atcaaagcgt cacagctttt ggaattaacg
     3301 ttgacgagca gaaatcgaaa tgcagaagaa cctattccta tgtgcggtgt acctcaccat
     3361 gcagcacaag gatatatcga tgccttgatt gaaaaaggct ataaagtagc gatttgcgag
     3421 caggtagaag atccaaagac gactaaaggg atggtcaagc gcgaagtgat ccagctagtc
     3481 actcctggta cagtcatgga cagcaaagga ttgtcggcaa aagacaacaa ttttttaaca
     3541 gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg ccgatttaag tacgggagaa
     3601 ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa atgaagcctc tgcattgcag
     3661 acaaaagaac tcgtattagg cagtgagatc actgataccc ttcgtgaaca gctaagcggg
     3721 cgtttagggc tagtcttttc tcaacaaaat gagatggaag aaaacgcgga atttagcttt
     3781 ttgacaagcg aactagtcga tccgctggaa aaagaagcaa ccggcaaact attgacttat
     3841 ttatcggtga cacaaaagcg gagtttagct catatccaaa aagcagtaga ataccaacca
     3901 gatcattttt tgaaaatgga ttattattca aaattcaacc tagaattaag ccaatctatt
     3961 cggactggga aaaagcacgg cactcttcta tggttgttag atgaaacaaa aacagcaatg
     4021 ggtggccgtt tactaaaaca atggctagac cgtccgctta tccaggcaaa acaaattaaa
     4081 gcaagacaag aaatggtcgc ttctttgcta gacagctatt ttgaacgtat cgatttgcag
     4141 tcagcattaa cgaaagtcta tgatctggaa cgcctagcag gtcgagtagc tttcggtaat
     4201 gtcaatgggc gagacttgat ccagctgaaa acttctttag aacaagtgcc aatgatcaga
     4261 gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc tgctttcaga aatgcagccg
     4321 atggaccatc tcgtccaatt gatcgaccaa gctatccaag atgatccgcc gttacaaata
     4381 acggaaggaa acatcatcaa agatggattc aatgagcagc tcgatatgta ccgttctgct
     4441 atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa aagagcgtca agaaacagga
     4501 atcaagaact taaaaatcgg atttaaccgc gttttcggtt attatataga aattacaaaa
     4561 gcaaatcttg gaaatgcaga actggagaag tatgaacgaa aacaaacttt agcaaatgca
     4621 gagcgtttca tcacaccaga actaaaagaa ttggagacac aaatcctcga agcagaagaa
     4681 aaatctgttg atttagaata tcagttgttt ttagctgtga gagaagaagt caaaaaggct
     4741 atccagccgc tacaagtact tgcaaaagca atcagtgcag tagatgtgct acaaagtttt
     4801 gcaacgatca gcgaacgcta tcagtatgtt cggccagaac ttgtcagtga caagcatcag
     4861 cttctgatca aagacggacg acatcctgta gtagaaaaag tattaggaca tcaggaatat
     4921 atccctaaca gcgtagaaat ggcagaagac gaaatgatct tgctgattac aggaccgaat
     4981 atgtctggta aaagtacgta tatgcgacag ttggcattaa ctgttctgat ggcgcaaatg
     5041 ggctgtttcg ttccggcaaa acaagcagtt ttacctattt ttgatcggat ttttacccga
     5101 attggtgcaa gtgacgactt aattgctgga caaagtactt ttatggtaga gatgatggaa
     5161 gccaatcaag ccctgcgtta tgctacgcca aacagtttga ttttgtttga cgaactaggg
     5221 cgaggaacag caacctatga cggaatggcg ttggcccaag caatcatcga atatatccat
     5281 cgtcatgtac aagcaaaaac actcttttct actcattatc atgaactgac ggtacttgag
     5341 aaagaacttc cgcagcttaa aaatgtccat gttggagccg ttgaaaaaga cggagaagtt
     5401 gtcttcttgc ataaaatgat ggatggacct gcggataaaa gctacggcat acatgtggca
     5461 aagatcgcag gattgccttc caatctacta gaacgagcag cagatatcct gcatacgcta
     5521 gaaaatggcg ataacggaca ccatactgtt ccagctgaag tagaagagaa aactaaaaca
     5581 gccaaacagg agcaaataaa accacctgca gaaaaagtaa atcaagacca tatacaagac
     5641 cgtgtaaaag aagaaaacca acagctttct ttattcagtg aattatccga aaatgaaaca
     5701 gaagtcattg aatcactgaa gcaaataaat ttactagaaa tgacgccaat ggatgcacta
     5761 aacacattat atgaattaca aaaacaactg taggaagaag gcggatatcg atggccaaaa
     5821 tccaagaatt atctgaacgt ttagctaatc agatcgctgc gggagaagta gtggagcgac
     5881 cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga cgccggcagt actcagattg
     5941 atatcttagt tgaagaagcg ggactgcgga cgatccaagt gattgataac ggagaaggca
     6001 ttttggcaga cgacgtagag aatgccttca agcggcatgc gacaagtaag atccataatc
     6061 gcgacgatct ttttcgcata cgaacgctgg gctttcgagg ggaagctttg ccaagtattg
     6121 cctctgtttc tgaaatgact ctggaaacgg cgaatcagga agaaaaacaa ggcacctatc
     6181 tgtcgctgaa aggcggagaa gttattgagc atcgaccagc accactgcgt caaggaacga
     6241 agattaccgt cagcaatctg tttttcaata cgccagcccg cttgaaatac gtgaaaacac
     6301 tgcacacaga attagcgaac atcggggaca tcgtcaatcg actggcattg agtcatccat
     6361 cgattgcttt tcgtttagta catgatggaa acaaaatgct ggcaactgct ggaaatggcg
     6421 atctgaaaca aacaattgcg ggaatctacg gattatctac cgcaaaaaaa atgctgaaaa
     6481 tcgaaggaaa agatctagat tttgagttat ttggctatgt ttcattgcct gaggtgactc
     6541 gtgccagtag gaattactta tcgacgatca tcaatggacg ttttatcaag aacttttcct
     6601 tgaataaagc aatcgttgca ggttatgggt caaaattaat ggtcggtcgt ttccctattg
     6661 ctgtattgga aatcaaaatg gacccgcttt tagtagatgt aaatgttcat ccgacgaagc
     6721 aggaagtccg gctgtcgaag gaaaaagaac taacagagct aattagtaac gccattcaaa
     6781 aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga taatctacgg tttaagaaaa
     6841 aagtacctga tcagcctaaa acagaacaaa tcgaaatcga tttgtcctac gcttttgaag
     6901 aagaaaaacc taaaaagcca agtagtttga attttgatcg agagacaggt acctttttcg
     6961 tagaagaact aaagaacaca tttgttgaca gaattgttga taagtctgtg gaaaatgttg
     7021 aaaactattc tggtttttct gaagaaaaca ccacttatcc agaaaaaaca gcgaaaacgg
     7081 tggataagtt tggtgaaaat gttgataact ttgtagaaaa aacagtggaa aaatctgaaa
     7141 agaaagaatt tcccattgtg gataacatgg agaaaacaaa tgaagaacaa gcagataaat
     7201 ccgtagaaga ggatgaaaag atttacgagg aagaattgac actccatcct gaatttgatt
     7261 tcactcaagg aaacgacaaa acattagaac aagccatcaa gaaattagaa acggaaaaac
     7321 cagtacaacg ttttcctcag ttggaatatt ttggacagat gcacggaacg tatctttttg
     7381 cccaaagtag cgatgggttg tacatcattg atcagcatgc tgcccaagag cgcattaagt
     7441 atgaatattt ccgggaaaaa attggggaag tatctgatga tctgcaagaa ttgcttgttc
     7501 catttgtgtt agactatcca aacagtgatg ctttgaaatt aaaagagcaa aaacacttgc
     7561 tagaagatgc agggatttat ttagaagaat ttggtcaaaa cagctttatt gtacgggccc
     7621 atcctacttg gtatcccaat ggagaagaag aatcgattat tcgggagatg atcgatatgc
     7681 tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga agcaacagca atcatgatga
     7741 gttgcaaacg atcgattaaa gccaaccatt atttgaatga agcacaagca cgtgtgctgc
     7801 tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc ccatggccgt ccagtattga
     7861 ttcactttac caattcggat atggaaagga tgttcaaacg aatccaagac ccgcattaag
     7921 tcaaagagaa gagggaaaac atgaagattg tattagcatc ccaatcacca agaagaaaag
     7981 agttattggc tcatatcgtt tcagaattcg agattgaacc tgctgatatc gatgaaacgc
     8041 catttccaga cgaacagccc gtcgattatg ttcgtagaat ggctgaagaa aaagcacgaa
     8101 ctgtctggga acaagatgaa aaagaagacc atttagtgat tgccagtgac acgactgttg
     8161 ttctcaatca agagatcatg ggtaaaccag aagatctggc tgaggctgaa ttcatgctga
     8221 aaaaactaag cggggaaaca catactgtat atacagctgt tgttttaaaa acaaaggaac
     8281 gagaagaacg gatactagca gaagcacatg tcacttttta tcctttgaca gacgaagaga
     8341 tcaaacgcta cttggagacg ggggattatg cggataaggc aggggcatac ggcatccaaa
     8401 atgctgcgag tgtattcgtc aaagaaattt ctggagatta ttacagtatc gtaggatttc
     8461 cgattggtgc tgtcaatcag gcactgaaaa cattcattta aacaaaaaag cggactatct
     8521 gattttcgat tgaaaatcca tgatagcccg ttttttttat tgaaaagatt acttatctac
     8581 tctgactaag attttacttc agctttaccg actgcttcgc ccgggcgaac aagtcctgat
     8641 tggtcaagag aaagtcctgc aactttttca ttattggtca aagcaacaat aatgtcagtt
     8701 ttcttgccag cttgttctat ttctggcaaa tccatttcag caatcagggt ctctggtgtg
     8761 acaaggtatc cttctttcac gaatacattg aaaggaacac cctttagttc aacagtatct
     8821 aatcccatgt gaacaagtac ttccagcccc tcattagaaa gaataccaat ggcatgtttt
     8881 gtttcaaaga cactcgtcac tttaccgttt actggtgcat agactttgcc gtttgacggt
     8941 tcgacagcat aaccgtcacc catcattttt tgtgagaaaa ctgggtcatt tacttcagtt
     9001 atttccttga tggtccctgc agcgactgcg aataatgttt cttttgttgc aactgccgta
     9061 ccagttgctg aaacaggttc gaaagactgt ggcgttgctg ttgcgccatt tgctgtttga
     9121 agttgtacat cagcagcatt ttcaggaaca gcaggatcaa tcttattcaa aattccctga
     9181 cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag caatgatcat tgcgatgatg
     9241 aaaggtaccc atgtttctgc ttggatagct agtattcctg gcaaaccgcc tactccgatc
     9301 gcgttagctc gaacacccat caagttagca aacatgccag ctaatccaga gccgaccatt
     9361 gcagcgacga aaggatagac gtatttcaaa ttgattccga acatagcagg ttcagttacg
     9421 cctaagtaac aagaaatcat tgctggaatc gagatttgtt cttctttttt attcccacga
     9481 tgtaagaaga tgatggctaa gactgcagag ccttgggcga tatttgataa agcaatcatc
     9541 ggccataaat tcgttgatcc gaaatctgca atcagctgca tatcgatggc attggtcata
     9601 tggtggagac cagtaatcac taaaggagca tacaggaatc cgaatacggc actaaacaac
     9661 cagctgatag aagacgtcaa tccagtattt acgatatttg agatccagct tccgactgtc
     9721 cagccgatag gacctaaaat aacgtgtgca gctaaaacag ttggtaacaa tgaaaacaat
     9781 ggaacaaaaa tcatcgagat cgactgaggg atatatttgc ggaagaagat ttctagatac
     9841 gctaacataa accctgccag catggctgga ataacttgcg cttgataacc aatcatctgg
     9901 acttgagcaa aaccaaaatc ccaaaatgga atatctgctg cggctgtgga agctacacta
     9961 taagcattta ataattgcgg agaaactaac gtaattccta aaacgattcc taatatttgt
    10021 gtagtaccca tcttttttgc aatactccaa gtgataccta ctggaagaaa atggaaaatt
    10081 gcttcaccgg gtaaccacaa aaaggcattg acaccattcc aaaacttcga gtgttcaacg
    10141 attgttccac caagactttc aaactggata ccttcaagta catttctgaa tccaaggatc
    10201 aatccaccga caatgattgc aggaatcagt ggtgtaaaga tttctgctaa aacggcaata
    10261 gcacgttgaa cgggatgaag gttttgtttt gcagctgctt ttccttgttc tttggaaacc
    10321 ccttcaactc cagatactgc aacgaagtcg ttataaaaag tagaaacatc gtttcctatg
    10381 atgacttgga actgtcctgc attcgtgaac atccctttta cgctaggtat atcttcgatc
    10441 gctttttcgt ccgcttttcc tggatcattc aggacaaaac gcatacgagt cgcacaatga
    10501 ctaactgcag caatattttc ttttccgccg atatcatgta acagcttctc ggcatctgct
    10561 tgatattttg ccatatcaat aaaccccttt ctcttaactt gtctatgcaa gttgtttctt
    10621 catttactat actttttttc ggagagaatt gcaagcgttt tattaaaaag tacgagagaa
    10681 aatgatatcg ttttaaaaag cttaaaataa gcttttctta aaaataggaa cattgaaata
    10741 caaaaagagc tgtagtatag tgaagaaatg atggaagaac gagtgaggga aaaaacatga
    10801 ataagttcaa tgaaattttt ttagatttgg aacagaaaat tttagcgaaa gaatatcctc
    10861 cgcattcgct tttacccagt gagaaccaat tgatcaaaat ttatggtgtt tcacgagaaa
    10921 caattagaaa agcgctgaat cttttgacta cggaaggtta tattcaaaaa aaacaaggaa
    10981 aaggttcgat cgtactggat cggaatcgct tcgactttcc tatttctgga ttgactagct
    11041 ataaagagct acaggagacc caacgtattc caagtgaaac gatcatccat acgttagaag
    11101 aaacagaagt gacaaaagca ttgaacgaga tcactggctg ggagatcggt gcgcctgttt
    11161 ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt tatattagac accgattatc
    11221 tattaaagga aatcgttcct attttaactc cagcacaagc acaaggatcg atctatgaat
    11281 attttgaaaa tgagctttct ttgacgattg cttacgcgca aaaggagatc acagtagaag
    11341 aagtaacgga aatcatgaaa acagcgatgg atcttcacga tgacacttat gcagtagtag
    11401 tcagaagcct cgttcatctg gaagataccc gctgtttcga gtattctgag tcggttcacc
    11461 gattagataa attccgtttc gttgagtttg cccgacgccg aaaagtgtaa atagttcaaa
    11521 agaaagcaaa ttgttggcat ctgtccactt tggcgctatg ataggatcac aaagggggca
    11581 tttttaatgg ataaaccaat aagaccagaa aaagaagaac tgaaagaaaa gttatcaccc
    11641 atggcttatg cagtcacaca agaaaatgca acagaacgac catttagcgg gaaatatgat
    11701 gatttttacg aaaaggggat ctatgtagat atcgtcagcg gtgaaccgtt attcagctct
    11761 gcagaaaaat atgatgcagg ttgtggctgg ccatcattca gcaaaccaat aacgaaaaga
    11821 caagtgcgcg aaaaagccga tttttctcat ggcatgcatc gaatagaagt tcgaagcaaa
    11881 gaagcagatt ctcatctcgg acatgtgttt accgatggac cagtagatca aggtggtcta
    11941 cgttactgca tcaattctgc ctcactgaaa ttcattccat atgaaaagat ggatgaatta
    12001 ggttatgggg aattcaaatc acttgtagaa taatggaaat acacaaagag cggtacaata
    12061 cggggattta ctcgatcgtc cgctcttttt ttattcaagc gattgctatt tgaaagaaac
    12121 gctccctaga ctcctttttt cacaaggtga aatgtgctac aataaaggga acgtatgtgt
    12181 gcaaaagaaa gaggactaga atatgtatga atatctgaca ggtgtggtta cctttatcaa
    12241 cccctattac atagtagtag aaaccaatgg tatcggctac cagatcgcac taggaaatcc
    12301 ttaccgctac agtagcaaac taaatcaaaa aataaaaatt tatgtccatc aggtaatacg
    12361 tgaagatgcg catcttctgt atggatttga ttcgctagaa gaaaagcaac tgtttttgcg
    12421 attagtaagt gtttcaggaa tcggtccgaa aagtgccctt gcaatcatgg caagcgaaga
    12481 ccactccggt ttgattcaag cagtagaatc cagtgatgtc acttatttga cgaaatttcc
    12541 aggagtaggg aaaaagacag cacaacaaat gatcttggat ctaaaaggaa aattcggtga
    12601 gctgagtatc gatacacctt ttagtctatt tgatgaagca aacacaaaag acgctacagc
    12661 tctatctgaa gcaatggaag ctctctcggc tcttggttac agtgatcgag aaatcaaacg
    12721 tgtagaaaaa caattaaaag aagtagataa tcagacaacg gatgagtatc tacgccaagc
    12781 actcaagcta atgatgaaaa aataaggagg agaccaaatg acagatgaat acttgttatc
    12841 accagaatct ggcgaaaatg aactgagttt ggaaaaatct cttcgaccac agctgctttc
    12901 tcaatatatc ggacagagaa aagtcaaaca agaacttcag atttacatcg aagctgccag
    12961 aaatcgggaa gaacctttag atcatgttct gttgtacggg cctcctggat taggaaaaac
    13021 gacgatggcc atggtcattg ctaatgagat gaccgtaaat attcgtacga cgagtggccc
    13081 tgctatcgaa cgtccgggtg acttagtagc aatcttgaat gaactggaag caggggatgt
    13141 cctgtttatc gatgagatcc atcgattacc tagagtggta gaagagatgc tttattcagc
    13201 tatggaagat ttctatgtag atatcatggt aggacaaggg ccaaccgcac atccggttca
    13261 tttccctctt cctcccttta ctttgattgg cgcgacgaca cgggcaggta tgctttctgc
    13321 acctttacga gatcgatttg gcatcatttc tcatatggaa tattatgacg aacaggatct
    13381 gcaggagatc gttttacgtt cttcggatat ttttcaaaca gagatcattg aagaaggggc
    13441 attggaaatt gctcgccgat cacgaggaac gcctagaatc gctaatcgct tgctcaaacg
    13501 ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc gatcgtccta tagcggatca
    13561 ggcacttact ttgctacagg tcgatcaggc tggattggat tatgttgacc agaaattgtt
    13621 gcgaacaatg atcgagttat atggcggcgg accagtaggg ttaagtacgc tttcagtaaa
    13681 cataggagaa gagcgtgaga cggtagaaga tatgtatgaa ccgtatctga tccaaaaagg
    13741 atttttaaag cgtacaccta gagggcgaat tgctactgct tatgcctatg aacattttgg
    13801 ttacgactat catatatgat ttttgctttt agattggagg aaaatgaatg agtgatacaa
    13861 cacgtacgaa acaattgatc aaaaacgtat tgattgattt gtgccaaacg caatcttttc
    13921 gaaaaatcag cgtccaaaaa atcgcaaacg aagcaaaaat caatcgacaa acattttatt
    13981 atcattttac agataaatac gatttgcttc gttgggtcta ttgtgaggat gcattgcatt
    14041 atttagatcc acaatgtctt tcacttgata attgggaaga gcaagcattg aaaatgctga
    14101 aagcaattgc tgagaagaaa gaattttatg caaatactgt gctttctgac ccagaaattt
    14161 tacaaaaaga gttttctcaa ctgatccaaa aatcatttat caagattttt gaacaaatgg
    14221 atgaagaaag acaactttct gcaagagaca aactatttta tgctcgtttt ttttcttacg
    14281 gttgcagtgg tgtattagtc aattggatca cgaatgaata tgaagaaaca ccgatggaaa
    14341 tcgctatgca gcttttccgt ttagccaagg atacggaatt atattcttat cgtctttatg
    14401 caaaagaaga gttggaagaa cagtaataga aaaatttttt gttggaaaag caataatcgc
    14461 taaaaggaga tggggaaatg acaaaaattt tatttgtttg tttgggaaat atttgccgtt
    14521 caccgatggc agaaggcttg ctgagaaaac gaatagcatt agaaggaagg gaagcagaat
    14581 tttttgtgga ttctgctgct acaagcacgt atgaagttgg aaagacaccg caccctggaa
    14641 cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa tatggttgct cggcaaatca
    14701 caccccacga ttttgaaact tttgattgga tcattggcat ggatcaagaa aatgtagaag
    14761 aactgaaacg aagagcacca cgctcagcgc aggggaaaat ccatttgttt ttaagtagca
    14821 ttcctggaaa agaaaaagaa aatgttcccg acccatacta taccaataac tttgatgaga
    14881 catatcactt gatcacagaa ggcctgaatc attggtggaa tatttggatt gatgaaaata
    14941 aaataacaaa caatttgtaa agttaacgct ttctttttaa ggcgatgtta gagcatttcc
    15001 ttgttttttt gtaaaatgtt aggtaaaata aaattaatta agtaacactt tcttagctaa
    15061 aggaggagtg cattcgatga acgaagagac agtatcgcca atgaagaagc ttacgaagtt
    15121 agttactcat acttcgtatc aactttttga ttcttttgta aatgacactg aagaacatgc
    15181 taagaatgta cgattgcaag gaaagtttat tcttgagctg caaaaagctg ctactgaaca
    15241 tagtctagtt atcttacaag taagagcaaa tcgtttgagt agcaagtatg aaacgatctc
    15301 aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg atgatccgcc tacaaaacga
    15361 agagcagcag atccggatgg ttcctgtaga acaaattgaa aaaataacaa tcctttcacc
    15421 agcaggggaa caggaacggt tatcaaaata atttatacca gatagaaaag cacgtatgag
    15481 cttgtaccat caatcattac taatcaaaaa taaccgaatg ctttaagctg atatcagtaa
    15541 aacggaacgt tttaggatga gtatcacagc atgacagcat tgggttgatt agttgaatcg
    15601 attggatggt acaagttttt ttgataaaaa aaggtcctgc tagccgggga gctagcagga
    15661 taaggagtta aaaatgaaaa agtgttgtta gggttgtttg ttggtatgct tatataatag
    15721 tcactagttg tgaatatttt gtgacgaaag ttttcgaaag ttgttaagtt tttttgcgaa
    15781 aactattttt atatcatata tatgtctaaa atctatatta taaaagaaat gttgtttgat
    15841 cagcttctct tcggtattag ttcaaaacaa aacggcaaag tatatggagc tactttgccg
    15901 ttttgtttct ctaatactca ggtcagttta tagacgctca ataaacggtg ttcaaaagcc
    15961 agtttctcgg tattaagtca aatttatgca aaaatgtgag aagccatttt caaaaaatat
    16021 ttcttaatac tcgaaactga gcggcttttt cacgacctct caataaacag tgttcaagag
    16081 acagcctatc ggcaataagg ctgaacgtct ctttcacaac ctcttttatc aatctgctac
    16141 ttcttctttc ttagcgaaac tccaaatcag tccgataaca aaaccgataa ctgctggtac
    16201 gatccacccc atccccaatt tgaaaaacgg caagtagctt tccgcaacat ataaaagatt
    16261 ttgaacaata ggtgtttgtt tgatagtttc tgggcaagcg ttcaacccat caaaaatcga
    16321 agcaatcaat gtaaaataag tagtcattcg ataaacagcc gtacgctgct taaacaaagg
    16381 accaactaac actagtaaaa tcagtgtcat tgctaatgga tagatgaaca tcaaaacagg
    16441 caacgaaaat tgaatgatat tcgtcaagcc tacgtttgcc gctaaacaag gaaggatact
    16501 agcaagtgca acaaagaatg cgtaatttct ttgcggaaac aaatctgtga atgtttctgc
    16561 aaaagctgta atcaaaccaa taccggtctt caagcaagct aatataacga taaatgctaa
    16621 taagatactg ccataagtac ctaaataatg atcagcaatc tgagccagcg tgatcccgcc
    16681 attttcactt agagcaaagc tacctaagct catagcacct aaataagcga gcaaagtata
    16741 aatgattccc atcaatataa tgctgatcgc accagatttg atcgtgtctt tagcgatttc
    16801 agcaggtttt gttacaccca tgttttggat tgttgtgaca atgatgatcc caaatgctaa
    16861 agcagcaagc gcatctaatg tgttatatcc ttgagtaaac cctgtgaaaa aagcatgctc
    16921 ttgataattt ggctgaatag gcgcttgatt tacagaacct agtggattca aaaaagcaag
    16981 gagaagcaaa gcacctaata aaaccaaaaa tgcaggattc aggaactttc caacataatc
    17041 taatatcttt gtcggttttc ttgaaaacca ccaagccagc acaaaaaata caatactgaa
    17101 aattgctaga tatagcgtct gtctgctagg atcgataaat ggtgctaaac caatctcgaa
    17161 agatgtggtt gcaaggcgag gtaaagcaaa aaacggtccg ataactaagt aaagcaagat
    17221 ggtaaaaacc atcgcatatg tttgatttat ccgttttgct aattcatata cacctgagct
    17281 tttcgataca ccaatggcaa tcacccctaa gaagggaaga ccgataccgg taaccaagaa
    17341 acctagattc gcccaaaagg tagcagcgcc tgcttcttga cccaaatgaa cgggaaagat
    17401 aagatttcca gcaccaaaaa atagaccgaa aagcatggag cctataaaaa gatagtcttt
    17461 aaatgataat ttttttgtca tacataactc ctttacggtt tttttaaaag taacaaaatt
    17521 tcgaaaaata tgcaacaatt ttcagaaaat ttcctatata aaacgacggt tatttaataa
    17581 cgtaagtgat aaaataaaag cgctgaatca tctgaggagg aatcatcgtg acaaagtcga
    17641 gattagaagc atttactgat ggagtggtag ccattgtttt gagagttctt gtattagaga
    17701 tcaagatccc taatacatct agctttcatt ctttatgggt catccgaaat acattattag
    17761 cttataccat cagctttata tttgttgcgg tgatctgggt gaaccatcat cgtatatttc
    17821 agatggcaga acgaattaat taccgagtgg tctggtcgaa tatctttttg ctcttttggc
    17881 tgactttatg tccttctgtt actagttggg tcgggcgtaa ccctgaagct ttttggcctg
    17941 agatggctta tgtcttagta tacacgatgt ggagtttttc ttttggtatc ttgatgaggc
    18001 aaatcatgaa agcaaataaa ccagattccc atgtcgtgag agttttgtca aaggaccgtc
    18061 gaagtcttat ctccatgatg attaacttat gcttgattgc cggtgtcttt tttgtaccgt
    18121 tgatcgggtt attcggacgt ttttttgtat ctggtatttg gattttttcc tataaaaaag
    18181 cagatgccta ttatcagcgg ttgtttccgg ggaaatagaa aagaggctgg gacagaagct
    18241 tttaactcca agaaataagc cgaaatttca caaaaattta agaagcaatt ttcgtaaatt
    18301 ccttcttatt tctcgaagct gaccacttct gtcacaacta ctttcttact tcttacgttc
    18361 atatttcata tgatagatta gtaatagaag caagaaccac acaggtgtta gtaataaagc
    18421 gattctcgta tcttttccta ggaacaagac gacacacaca aaggccatgt aaagaaatac
    18481 cagataatta ctaaatgggt acaacggcat tttaaatgaa tgagcttttt tgccgaggat
    18541 acttttacga tatttcatgt gcgtgtagac aagcatactc cagataaaca agaaacaagt
    18601 agtggcaata ctgcttacta gtgtgaatac ttcactaggc atcacgtaat tcaagataac
    18661 agaaatgaaa atgacgatag tagaaaatgt caaagcattc cctggcacat gatgtgttgt
    18721 taactttttg aattttttag gggcactgcc ttcttgcgct aaagaacgaa gcatacgtcc
    18781 tgtactatat attgcactgt tgcaggcaga agctgctgca cttaatacga ctaaattgat
    18841 caaggaagca gcaatcgtga tcccaatttc cgaaaatact tctacaaatg gactgttatc
    18901 tggattcaag ctgttccatg gatagattgc cataatgaca aaaagagatc ctaagtaaaa
    18961 taagatgatc ctgataggaa tattattgat agcttccggc aacacttttt caggattttc
    19021 tgtttctcca gcgactaatc caactaattc tatcccagca aaagcaaagg ttaccatttg
    19081 gaaagctaag ataaatcctt ttgcaccagt tggaaagaat ccgccatgag accatagatt
    19141 cgtgatactg gctgtgccaa tatctgtcgt atagtgggtg aggatcatat aagctcctac
    19201 gaggataaag gcaatgatcg ctacaacttt gatcaatgca aaccaaaact ctaactcacc
    19261 aaacaatgag acagccacca tattcaagca aagaagaaga cctaatgcaa tcagttcagg
    19321 aagccactga ggaacaccag gcgcccagta gcgtacatac attccgatgg ctgttaaatc
    19381 ggccattgca atcgataccc agcagaacca atacgtccag ccagtgatga aagccgcttt
    19441 tttgccaaga tattctgcaa caaaatccag aaaagaatgg ttattggtgt ttgataacag
    19501 aagttcgcct aacgcacgca taatgaaaaa acagatgcat ccagtaataa gataggcgag
    19561 caatatcgaa gggccagcta gctggataga ctttccggaa cctaaaaaca accccgttcc
    19621 aatcgctccg ccaatagaaa tcagttggac atgtctattt ttcagtccac gctgcagttc
    19681 ttgctcttca ttcattttta aatcatccta actaaaaaag tttaattact tccatattaa
    19741 aagagttttc tgaaaaacac aactaagatt ccaaaatttt cagaaattta ccttttatga
    19801 gtaattcata aaaaagaact gcggtaaaca ccgcagctct ttgtcccaat cagctattga
    19861 tcctaagcat ttaaaatacg tgaaaggaat tcttttgtac gttcttcttt cggatggaca
    19921 aagatatctt ctggtgtacc ttcttctgca atcacacctt tatccataaa aatgacacgg
    19981 tcagatacat cttttgcaaa ttccatctca tgcgtcacga taagcattgt caagccagta
    20041 tgtgctaagt ctttgatcgt attaagcact tcgccaacca tttcagggtc taacgccgaa
    20101 gtaggttcgt cgaatagcaa tacatctgga ttcattgata atgcccgagc aattgcgaca
    20161 cgctgtttct gtccaccaga taattgtgct ggtttggctt cgatataacg atccatacca
    20221 actttttcca gattttcgat tgctactttt ttggcagttt ctttatctcg tttcaggaca
    20281 gttgtttgac cagacataca gttttccaag acattcatat tgttgaacag gttgaaggat
    20341 tggaaaacca tacctagatt cgttcgatat ttaggtaaat tatatttagg agctaataca
    20401 ttttctccat tataaatgat ctcgccgcct gttggttttt ccagtaaatt gatacagcgc
    20461 aagaaagtag atttaccaga accggaagaa ccgatgatcg tcacaacttc acctttattg
    20521 actgttacgc tgatatcttt caatacttca ttttcaccga agctttttct tagatgtttg
    20581 atttcaataa ttttttcatt catgattctt cctccttatt cctgcaaatt tgaattgtcg
    20641 atttcttcaa gcttggtata agctgaagga ccatcgattt tcttttctac cgcccgcagg
    20701 attctggtga tcgtaaaggt catgactaag tagatgatcc cgacgatcgt aaatgtttgg
    20761 aagaattgga aattcgcacc ggaagcagag tttccttgga agaacaaatc agctacgccg
    20821 attacactta aaactgctgt atctttgata ttgataacaa actcatttcc tgttgctggc
    20881 aagatattcc gtaaaacttg tggaataaca acttttctca tcgtttggcc atgggtcata
    20941 ccaatggctt gcgcagcttc gaactgccct tcatctactg caaaaatccc cccacgaacg
    21001 atctctgtca tgtaagctcc agtattgaga gagacaataa acaaagcggc aatcgttcgg
    21061 ttcaatgaaa tgccaaaagc gagtgccaga ccgtagaaga tgaccattgc ttgtaccatc
    21121 atcggtgttc cgcggaatac ttcgacatag atagaaagaa gtgcatttcc gattttttgg
    21181 aagaagcgag tcagtgcatt tcctgattca ggtaccgaac ggaagacacc aatcagtaat
    21241 ccaagtgtag taccgacaac tgtcccgatc aatgcgatga acaaggtaag tccagcacct
    21301 cgtaagaaca tattgccgta ttgtttccaa atcgtagtga aatcagaaat gatgccaggt
    21361 ttttcttcac tatcagttgc agcaggctga tctttgactg cttggtccat aattttggtt
    21421 cgttcatcgt tagagatgcc agaaagaatc tggtttactt tttggatatc cgggtcgcct
    21481 ttacgcatac caacagaaat ctgaacatct tccgggtttg tctggaagcc atctgcaccg
    21541 ctgaattcaa gcatttccaa gtctttgttc acactagttg ccgttacacc ttccggacgt
    21601 tcgctgacat aaccatcgat cattccagaa gctaaagctg ttctcattgc tgagaaatta
    21661 tccattgctt gttgtttctg cacatctggt atctgatcga tcacagtata gtggaatgta
    21721 tttaactgag cagtaatctt tgctccagaa agatctgcta aactttttgc ttttgcatat
    21781 ttgctatctt tttgaactac tactactaat tgtgattcgt aataaggatc ggtaaaatca
    21841 atttcttttc gtcgttcagc agtagggctc attccggcga tgatcgcgtc gatcttgcct
    21901 gattgaaggg caggagccag accatcccat ttcgtttgga cgatgactaa ttttttccct
    21961 aaaccatctg ctactttttt agcgatttga acatcataac cgccggcgta actattttgg
    22021 ccttgaattg gcaaggcatt attctcatct gtttgttgag accagttgaa gggtgcatac
    22081 ccagcctcca tacctacgcg aaactcatcg ctcggtgttt tttcatctgc atttgccaga
    22141 gatcccacac tgaatagtga gaagacaaaa gcaaatagta cggcaactga aaccatattt
    22201 tttttcattt ttttctccct ttctgattat atgatacacg agcggtctag atgaaatgcg
    22261 gtaaagcttt atctgaaaaa ataaaaagcg accgatttat agatataaaa aggccgctaa
    22321 aattccataa ggtgataaac ctctcacaaa acatagcgca acttagcatc actgctaaga
    22381 cagtccgcaa gctcttaact tgcgtcccaa catacactcc aagcaaaaaa gtgcacattt
    22441 cggcgatcct ccctattcct gcttctccat gtttgcttca ctccacaggg ttaatgattc
    22501 tcgcgacctc tacctcattg attgaggcat ctcgtattca gttatttata caaaaaaagt
    22561 ttacgatact cgagaggctt tgtcaatcta taactgctgt tttttgaaaa aatactgata
    22621 aatatagaaa tctttacttt taccgaaaaa acaatcaaaa tgtgaatcat ctatccattg
    22681 caaacgatag tataattata cacaaaaatg tatgtaatac aataaaaaaa gaactagaac
    22741 agaaaatttc ctgtgtccag ttccatctcg atcatttaag ctgccgcttt ctttggctca
    22801 cgtcctagga tcgcttgcaa aagaacaacg acgccataaa tggctaatat tagataaatc
    22861 aaacttgagt attgggtgat tccccagccc catgttcgat atacggctaa tgtaatggct
    22921 aaaacaattg cggcaatcac gttgaataaa gcaaaagccc aaagatttcc attttctttg
    22981 cgtacaagat aaaaaacaga gaaataaacg cttaacgcta accaggcgat acaagccaga
    23041 ataacgcccc aataaataaa gaatggtaac atatcgattc acctcacttt tatacagcag
    23101 tattgtaaca cgttttcatt cttttgtcag tgaacattgt gataaattgt tcaaatgccc
    23161 ataacacttt tttattccca tttttctgac ttttcaacaa taaatttgtt cgagtatggg
    23221 ctataatgga aagtgaggtg acaaaacatg aaacaattaa cagtagacga agcagcaaaa
    23281 gagatattag cttatcagca aattacattt ttgaccggtg caggggtatc cacaccttct
    23341 ggagtgccag actatcgatc tttgtcagga atctaccacg ggatggagca gccggaatac
    23401 ttgctgagtc atcaagcaat ggtgaatgaa ccggataaat tctatcaatt tgtcaaaaag
    23461 atttatcatc ctgaagcaaa acccaatatc attcatttgg aaatggcaca tctagcgaaa
    23521 gaaaaaaatg tttgggttgt ttcccaaaac atcgacggtt tgcacgcaaa ggctaaaagc
    23581 ccgcaactcg tcaattttca tggaagtttg tatcaatgtc attgtcgaaa atgtcaacaa
    23641 acggtagact ggaaagaata tcttcacagc gacaaacatc aagcatgcgg aggacaaata
    23701 cgcccagaca tcgtgttata tggtgaaggg tttcaagatg gagtcatgga gcaggcggct
    23761 tatgccgtca gccaagcaga gctgatcgtc attgttggaa cgagttttca agttcatccg
    23821 ttttgtgatc tggtacagtt tcgccagccg tcagccaaaa tcttagtaat caatcaaacg
    23881 cctgtttatt tagcggagcc ttatacgttt attcaagaag acggagcggt tgttttcaaa
    23941 aaaatacaag aattaggagc agattatgac tgaattcaaa accgaaaaag aaaaaatgat
    24001 tgcaggcgag ctttattttg ccaatgatcc tgagctggtt gccgaccgta tgtttgcaag
    24061 aagtcaaagc caaatcatca accaagcaga atcagcggaa ctgcgatccc aattgttgaa
    24121 agagactttt ggccgtacag ggaaaaaaat ctatatggag ccagttatca attttgatta
    24181 cggatataat atatttgttg gggagaattt ttatgcaaat ttcaattgta catttttaga
    24241 tgtcagcacg attgaaatcg gtgataactg catgttcgct ccgaatgtcc agctttacac
    24301 ggcgacgcat ccactacatc cagtgaaacg taacagcggt ctggagtacg caaaacccat
    24361 taaaatcggt aataatgttt ggttaggcgg aggagttatc gtcacgccgg gtgtcactct
    24421 aggggacaac gtagtagtcg gcgcaggttc ggttgtgacc aaatcttttc ccgacaatgt
    24481 agtgattgca ggaaatcctg ctcgtatcat caagacagta gaggaagaac taacagaaga
    24541 gtctcttgaa acattacgtc ataagatcga tatgatcgat cgagagttga ttgctttatt
    24601 agaaaaaaga atggatacgg taactaaaat aggacaagtg aaaaaagaag aaaaccaagc
    24661 agtttatgat gcaaaaagag agcagcaagt attggataaa gttgttagtc tgctgaaaaa
    24721 taagggatat aaggaaacaa taacagatac gtatgttgat ttgatgaaac actcgcgcga
    24781 ataccaaaac aaaatgaagg aggaataagt gtgatcgaag gagagacacg gcgaaaacaa
    24841 atcatgcaaa ccttggcaaa tacagaaaaa ccagtgagtg ctagcaagtt tgcgaaaact
    24901 tttggagtca gccgtcagat cattgtaggc gatattgctt tactacgagc tgcaggtaaa
    24961 agtatcgtgg caacagcaag aggttatatc ctcgaaacag aagaaaccaa tactgggcac
    25021 atcagtaaaa tcgctgtcca gcatggaaaa gatcaaacag aagaagaact gagactaatc
    25081 gttgaaaatg gtggtgaaat cattgatgta attgtggaac atccccttta cggtgagtta
    25141 gcaggaactt tgcacatcaa aacgccacaa gatatcgatt cctttatgag acgttatcaa
    25201 aaaagtaatg ctactttgtt atccgaatta acaggcggca tccatctgca tacgatccgc
    25261 tacccagaaa agcatatgct gaaaaacatc aaacaaaaac tagctgaagc aggaatcctc
    25321 tacgaaggat aacatcgtag ctgctacttt ttatcctaga aactattcta tcatctctga
    25381 gataaacacc catatggaag aaataagaaa aaataagaaa agatgcgtat ccaatacaca
    25441 gaattttttg gaattgcgat ttctagatgt ctatctaaat ttcgattagt ggagaaacat
    25501 aaaaaaggga agtgcagctt ttgagcactc ccctttttta taaagctttc ttgtagcata
    25561 gtatcgaagg aacctgccaa ttctctactc gatcaccaat acgccttctt ttagtgcaaa
    25621 tggattttga tcgttgatgt ggtcatagaa catcacaccg ttgatatggt cgatttcatg
    25681 ttgaacgacg atcgattcat aattcttcaa acgaatcttg tgtttttcac cattcatatc
    25741 ataataagac acagtgattt tggcatggcg aacaacatat cctggtactt ctcgatcaac
    25801 agataaacag ccttcacctt cccctaaaca agcatcttga acagaatggc tcaaaatttt
    25861 aggattatac ataacagtac ttagagaagg tccatctgcc tctgggtcag gacttggtac
    25921 gtgaacagca atgattcttt tagaaatatc taattgagga gcagctaagc caacaccgcc
    25981 tcgtaagtgt agttcttcag ctttgattgg atcttggctg ttttttaaga actcaagcat
    26041 ctcttttcct aaagagatat cttcttcgct taatggaaga gaaacttctt ttgcaacttc
    26101 gcgtaaggtc ggatttcctt cacgaatgat atcatccatt gtaatcatgg aatatgtacc
    26161 cctttcttaa aagactgtgt ttcaagtcta ttttcgcatt atagtttacc acaaaaatgg
    26221 aaaagtagta gtaagattgt ttgaaaacta ccttttctta atgattaact acttgatttt
    26281 caacccggtt tcaggtaaag tatagctgtg tgctctaggc acatgacctg ccacttggtt
    26341 aagcgaatca ccaacgtttt actcagtcgc aggggaataa tcgaaagagg tgaaagaaat
    26401 ggcaatttca aaagaacgca agaatgaaat catcaacgaa tacgctcgcc atgaagggga
    26461 tactggttct ccagaagtac aaatcgctgt attaactgaa gaaatcaacc acttgaatga
    26521 acatgcacgc gtgcacaaaa aagaccatca ttcataccgc ggcctaatga aaaaagttgg
    26581 tcaccgccgt aacttgttag cttacttacg taaaactgac gttcaacgtt accgcgaatt
    26641 gatcaaacgt ttaggcttgc gtcgttaatt ttagtcaaaa agagtgggat tcacatgaat
    26701 ctcacttttt ttactcgtat atcaattaag taaaattccg tgatcaagct agatggactc
    26761 ctactaacca aatgaaagct gctcaaaaga cggctttcgg cagttttcat ttgtttagta
    26821 gcgtcttgct ttcacgagaa ggaaaaggag aagttatgtc agaaaaacaa gtattcaaaa
    26881 cgacatgggg cggccgtccc ttacaaatcg aagtcggtca gctagcaaaa caagcaaacg
    26941 gagccgtttt agtccgttat ggagatacag tagtcttaag tgcagctgtg gcctctaaag
    27001 aagcaaaaga tacagacttt ttcccattaa cgatcaatta tgaagaaaaa atgtatgcag
    27061 cagggaaaat ccctggaggt ttcatcaaac gagaaggccg tccaagtacg gaagcaacat
    27121 tgactgcccg tttgatcgac cgtccaattc gcccgatgtt tgcagaaggc ttccgtaatg
    27181 aagtccaagt aacgaacatc gtgatgagtg tagaaacaga ctgctcgcca gctatggcag
    27241 caatgctagg ttcttcttta gcactatcaa tctcagatat cccatttgac ggcccaatcg
    27301 caggcgtaga agttggtcgt gtaaatggcg agtacgtctt gaatccaaca gtagaacaag
    27361 cagaacaaac agatatcgaa ttgactgttg ccggaacgaa acaagccatc aatatggttg
    27421 aaagcggcgc gaaagaagtt tcagaagaag acatgcttgg tgcattgctg tttggttttg
    27481 acgctatcaa agagttagtt gctttccaag aagaaatcgt tcaagcagta gggaaagaaa
    27541 aaatggaagt gactttactg caagttgatg aagtattgaa aaaagaaatc tttgatgctt
    27601 cttacgctac aatgaaagca gcagtaatga ctgaagaaaa acttgctcgc gaagataata
    27661 tcgagcaggt gaagatcgat atccgcgaag cttatgctga gaaatttgca ggtcatgaag
    27721 atgaagatca cttgttgaaa gaagttaaac aaatcacaga agatcttgaa aaagatgtcg
    27781 tacgtgaatt gatcaccatc gataagatcc gtcctgacgg gcgtaaattg gatgaaatcc
    27841 gtccattgtc atcagaggtc agcttattgc cacgtgtaca tggatcaggt ttgttcacac
    27901 gaggacaaac acaagcttta tcagcttgta cactcgcacc tctaggagag caccaaatca
    27961 ttgatggttt aggggtggaa gtcagcaaac gattcatcca ccactacaat ttccctcaat
    28021 tttctgttgg ttctacagga cgtgcaggct ctccagggcg tcgggaaatc ggacatggcg
    28081 cattaggtga acgtgcgtta gcacaagtaa tcccttcaga agaagaattt ccttatacga
    28141 tccgtttagt agcagaagta ttggaatcaa atggttcttc ttcccaagca agtatctgtg
    28201 ctggcacatt agcattgatg gatgcaggtg taccaatcaa agcgccagtc gccggtattg
    28261 cgatgggact tgtttctgat ggtgaaaact atacgatcct aactgatatc caagggctgg
    28321 aagatcatct tggcgatatg gactttaaag ttgccggtac aaaagatggt atcactgctt
    28381 tacagatgga tatcaagatt caaggaatca cagaacaaat cctaactgaa gcattaactc
    28441 aagcaaaaca agcacgtatg gaaatcttag aagaattgac ttcaacgatt gctgctccac
    28501 gtgaagaatt aagccaatat gcaccaaaaa tcgaaatgat ccagatcgaa cctgctaaaa
    28561 tcaaagatgt tatcggtaaa ggcggcgata cgatcaacgg aatcatcgat gaaactggtg
    28621 tgaaaatcga tatcgatcaa gatggaaaag taagtattgc ttcagctgat gcagaaatga
    28681 tcaaaaaagc tatcaagatc atcgaagatt tgaccaaaga agtcaaagtt ggtgaagttt
    28741 atctagggaa agttgtccgt atcgaaaaat tcggtgcatt cgtcaacttg atcaaaggaa
    28801 aagatggact tgtccatatt tcccaattag caaacgatcg tgtcaataaa gtcgaagatg
    28861 ttgttaaact aggtgatgag atccttgtga aagtaactga aatcgataaa caaggacgcg
    28921 taaatctgtc aagaaaagca atgttgaacg aagatggttc aacgaaaaat gaagaaaaat
    28981 aatttaattc ataggaataa tgtacttgtg atcgtgaagg tgacaggggg acaataatta
    29041 ttcgaaaaaa ggggactgtg acaaaagccg tgtcacagtc cttttttttc gaataaacgg
    29101 tgttcaaaag cttgcatctg cggtaataag tccagccaag ttaaaaacat taggagctgt
    29161 ttttatttgg ctgttcttat tacttgatgc agaacagctt ttttacaacc tctccaataa
    29221 acgatgttca atcagctgtc cctcgatatt agctcaaaaa acggcaaaat atgaggaact
    29281 atttttccgt ttgtttctct aatactcagg tcaaaccgct gatttcacaa cctctgattc
    29341 acggtgttcc agaagcagct tctacggaaa taagctgaac acttttgtta caacttcttt
    29401 ttttctggag tttcttggcg catatttttg gagtccttct gaataaagcg taaaataaaa
    29461 gtgagaaata tggataaagg agggaaagga gatgctgaat tttttatggt cgttatttat
    29521 cggcggtata ttaggtttga tagctggcgc aattttagga aaggaagtcc cgggaggcgt
    29581 atctggaaat atcattatcg gttttttagg tagttggctc ggcgaatttt tactaggacc
    29641 actgggaccg gaactcggcg gtttttattt gataccagca ttattgggtg caattttttg
    29701 ccttgctctt tattcatttg tggcaagacg tatcagagaa cgtccaaaga aagaataatt
    29761 ttattcaaat aagttgacac ttcagtgtgt tacttggata attagaagca atttaggaaa
    29821 gcgggtgtca tggataagaa tatcatctat cattcaagct tacctaaagg atatccatat
    29881 aacaagaaat gagggaaaaa cgtggcaaca acgcacaata ccaaaaagat atcaatgact
    29941 ggcctgcttg tagcgatggg agtcgtctac ggagacatcg ggacaagtcc gctttacgtt
    30001 atgaaatcga ttgttgaaga taatggcgga ttgtcaggaa tcaatcctga ttttgttatc
    30061 ggatcagtct cattgatttt ttggacattg actctactga caacaatcaa atatgtcgtg
    30121 attgcattga atgcagataa ccatggtgaa ggtggaattt tctcactcta cacacttgtc
    30181 cgaaaaggtg ggaaatattt gatcattcct gcaatgatcg gaggagctgc cttactagca
    30241 gatggggtat tgacccctgc tgtgacggta acgactgcga ttgaaggctt gcgaggaatt
    30301 ccgcagtttt ttgaccggtt cggtaataac cagaacgtga tcgtcattat cactttagtt
    30361 attatttttg ttttattcat ggtccaacgc tttggtacag atattgtcgg aaaagcattc
    30421 ggaccaatca tgttcgcttg gtttactttt ttaggcgtaa tgggattaat gaacttcagt
    30481 caagactgga cagttatccg tgcactgaat ccttattatg ctatccagct gctttttagt
    30541 gcagataaca aattaggctt gttcattctt ggaaatattt tcctggctac gacaggtgca
    30601 gaggcgcttt attccgatct tggacatgtc ggcaaacgaa atattcgtct gagctggcct
    30661 tacgtcaaaa tttgtttggt tatcaattat tttgggcagg ctgcgtggtt gttgaatgtt
    30721 tatcaaaacc ctgaagcaca gcaaatcaag aacctgaatc cattcttcca aatgatgcct
    30781 caaagttgga tcgtgatcgg cgttgctttt gcaacagtcg ctgcagtcat tgcttcgcaa
    30841 gcattgatta caggttcgtt tacgctagtt tcagaagcaa tcaagttgaa actgctgcct
    30901 cggttaaaga tcatgtatcc tgggaacagt atcgggcaaa tgtatatccc agcagtcaat
    30961 ctgattttat ggatcgcctg ttctctagtg gttgtcacat tccgaacgtc tcatcatatg
    31021 gaagctgctt acggtctatc gatcactgtg acgatgttga tgacaacagc actgctttac
    31081 ttttatctta tccaaaatgg gcattctcgt tggcttgcat atttagttac ctttttcttc
    31141 ggtgcgatag aagtcgtttt ctttatctca agtgttgtca aattcttccg aggcggtttt
    31201 gtagccgttc tgattggttt gatcatctta gtcgtgatgt ttatttggga acaagggaac
    31261 attattcgcg aatcgtcaac cgaggatgtg tctttgagag attatatccc gcagcttaga
    31321 gaattacgtg atgatacttc tttaccaatc tatcagacaa atgtggtctt cttggttcct
    31381 gatatggctg aagataaagt tggccgacag ttcatctatt cgattttaga taaacggcct
    31441 aaaagagcac gagtctattg gtttgttcat gtggaagtga cggatgagcc atatacgaaa
    31501 gaatatcaag tagatatgat ggacactgat tttattgtcc aagtgaatct gttcttaggt
    31561 ttcagggtcc aacaggaaat caacgtatat gttcgccaag tcatccatga tttgatgaaa
    31621 caaggccgtt tgccaaaaca gccacaaaga tactctctga caccagggcg agaagttggc
    31681 gatttccaat ttgtcctgat tgatgaagta gtttccaatg tgacaactct aggaaaatgg
    31741 caacgtcaaa tcatgcaggc aaaactggca atcaaaaaaa tcgctacctc gccagaaaca
    31801 tggtttgggc tagagtacag cgaagtaaaa catgaaactg ttccgcttat cattggaaat
    31861 actcggaaaa catggttgaa agagcggttg tgaaagtagc gctttgacct gaactaatgt
    31921 cgaatgtcag ctacttgaac accgtttatc agaggtcatg aaaaagctgt actgcaacaa
    31981 gcagtaagaa cgagcagata aaaacagctt ctcatgtttt tcatctgttt gggcttaatg
    32041 ccgtagatgc aagcttttga ataccgtttt tatcagaggt catgaaaaag ctgtttagca
    32101 acaagtaacg caatgatttt gaagtgataa ggagatgtgg ctgaaaaatg ccgcatctcc
    32161 ttttttattc ctaaatatga atacgcatta ttttcaatct ttttgtagaa aaagggtaca
    32221 atagaaaaag agaaaggcgg gagaatatgg ttatcaattt cattatttta tttttgatga
    32281 ttgcaattac ttcatttttt gtagcaagtg aatttgcttt agtgaaaatc agacgatcaa
    32341 gattggaaca actagaaaag gaaaatgtaa agaacgccaa gctagctctt catgttacac
    32401 accatttaga taactattta tcagctagtc aattaggaat tacgctaacc ggtttgatca
    32461 tcgggtgggt cggtgaagga tctgtcgcgg ctttactaga accagtgatc ggtaaactac
    32521 cttttagttc ggctattagc agtacaatct ctgtggcgct gggatttatt ttagttacgt
    32581 atatcgatgt ggttattggc gaactgcttc ctaagagcta tagtatcgtc aatacggaga
    32641 aggtcgtctt gttcgtagtt aaaccacttc attattttta taaagtaatg tttcctttta
    32701 tttgggtatt gaatcattca gctgcagggc tagggaagct attaggtgtg cgtctcgtat
    32761 ctgaaggaga agagacattg tctcaagaag agctgacatt agtcgcattg aattcttatg
    32821 aaaaaggaga gttgaccaaa gaagaatatt attatttgga aaacgtgttt gaatttgatg
    32881 ataccttggc aaaagatata caagtagacc gtacgtccat gcaagtattc gaagcttcag
    32941 aaaccgtcaa agaagcgata aaagcttcga ttgaacaagg acatactcgc tatccggtta
    33001 tcttagaatc aaaagacaat gttttaggtt atgtcacctt gccagatttg atcaagcaat
    33061 cttataaaga tgaccagctg acagtagaac agttgattga agagccgatc gtaacaactg
    33121 aaacgatccc tataaaaaaa ttattgacga tcatgcgaaa aaagggtaag catattgcta
    33181 tcttaaaaga tgaatacgga gggacaagcg gcttagtgac gattgaagat attttagaag
    33241 aaatcgtcgg ggaaatccga gatgagacag atttggacga agcattgata gcgga
//
LOCUS       DACCCS010000029        32592 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.029, whole genome shotgun sequence.
ACCESSION   DACCCS010000029 DACCCS010000000
VERSION     DACCCS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.029"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(133..306)
                     /locus_tag="IVK82_001411"
     CDS             complement(133..306)
                     /locus_tag="IVK82_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6392483.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="IVK82_001412"
                     /pseudo
     CDS             754..3116
                     /locus_tag="IVK82_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="IVK82_001413"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="IVK82_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP6392484.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="IVK82_001414"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="IVK82_001414"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP6392485.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="IVK82_001415"
     CDS             5704..6489
                     /locus_tag="IVK82_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6392486.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="IVK82_001416"
     CDS             6502..7353
                     /locus_tag="IVK82_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP6392487.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="IVK82_001417"
     CDS             complement(7477..7929)
                     /locus_tag="IVK82_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6392488.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="IVK82_001418"
     CDS             complement(8394..8762)
                     /locus_tag="IVK82_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP6392489.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="IVK82_001419"
     CDS             8912..9394
                     /locus_tag="IVK82_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP6392490.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="IVK82_001420"
     CDS             9431..10441
                     /locus_tag="IVK82_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP6392491.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10556..11269)
                     /locus_tag="IVK82_001421"
     CDS             complement(10556..11269)
                     /locus_tag="IVK82_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392492.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(11459..12049)
                     /locus_tag="IVK82_001422"
     CDS             complement(11459..12049)
                     /locus_tag="IVK82_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP6392493.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            12533..12760
                     /locus_tag="IVK82_001423"
     CDS             12533..12760
                     /locus_tag="IVK82_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP6392494.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12776..13942
                     /locus_tag="IVK82_001424"
     CDS             12776..13942
                     /locus_tag="IVK82_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP6392495.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            14176..15219
                     /gene="hrcA"
                     /locus_tag="IVK82_001425"
     CDS             14176..15219
                     /gene="hrcA"
                     /locus_tag="IVK82_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP6392496.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            15313..15876
                     /gene="grpE"
                     /locus_tag="IVK82_001426"
     CDS             15313..15876
                     /gene="grpE"
                     /locus_tag="IVK82_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP6392497.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            15927..17756
                     /gene="dnaK"
                     /locus_tag="IVK82_001427"
     CDS             15927..17756
                     /gene="dnaK"
                     /locus_tag="IVK82_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP6392498.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            17907..19073
                     /gene="dnaJ"
                     /locus_tag="IVK82_001428"
     CDS             17907..19073
                     /gene="dnaJ"
                     /locus_tag="IVK82_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP6392499.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(19155..19820)
                     /locus_tag="IVK82_001429"
     CDS             complement(19155..19820)
                     /locus_tag="IVK82_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP6392500.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            19796..19969
                     /locus_tag="IVK82_001430"
     CDS             19796..19969
                     /locus_tag="IVK82_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6392501.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(20014..20751)
                     /locus_tag="IVK82_001431"
     CDS             complement(20014..20751)
                     /locus_tag="IVK82_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6392502.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(20753..22255)
                     /locus_tag="IVK82_001432"
     CDS             complement(20753..22255)
                     /locus_tag="IVK82_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6392503.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            22514..24508
                     /gene="uvrB"
                     /locus_tag="IVK82_001433"
     CDS             22514..24508
                     /gene="uvrB"
                     /locus_tag="IVK82_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP6392504.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            24518..27334
                     /gene="uvrA"
                     /locus_tag="IVK82_001434"
     CDS             24518..27334
                     /gene="uvrA"
                     /locus_tag="IVK82_001434"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP6392505.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            27597..28862
                     /locus_tag="IVK82_001435"
     CDS             27597..28862
                     /locus_tag="IVK82_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP6392506.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            28866..29597
                     /locus_tag="IVK82_001436"
     CDS             28866..29597
                     /locus_tag="IVK82_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP6392507.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            29732..30616
                     /gene="rapZ"
                     /locus_tag="IVK82_001437"
     CDS             29732..30616
                     /gene="rapZ"
                     /locus_tag="IVK82_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP6392508.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            30613..31611
                     /locus_tag="IVK82_001438"
     CDS             30613..31611
                     /locus_tag="IVK82_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP6392509.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            31645..32580
                     /gene="whiA"
                     /locus_tag="IVK82_001439"
     CDS             31645..32580
                     /gene="whiA"
                     /locus_tag="IVK82_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP6392510.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
BASE COUNT        10826 a         5599 c         6939 g         9228 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat
    10561 tatctataac tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag
    10621 ctactacaga tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag
    10681 atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt
    10741 catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca
    10801 catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat
    10861 aatgaataac atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag
    10921 ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg
    10981 actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg
    11041 tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat
    11101 tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga
    11161 ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc
    11221 ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct
    11281 cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt
    11341 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
    11401 tcatcagtaa aattacttgt cacactattg ataaggagta tttaattgaa gagaatgatt
    11461 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
    11521 tcgttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
    11581 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
    11641 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
    11701 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
    11761 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
    11821 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
    11881 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
    11941 tagaagcagt tttgttttgc catttctcat tttggtttag tgtgctagta atataagcac
    12001 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
    12061 cctttactat ctataacaag gacaacagtt ttaacctaca gacattcatt aaaattgggt
    12121 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagata gtcaacttaa
    12181 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
    12241 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tattaaaaga
    12301 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
    12361 tgaaaacaac cctagagata tataactagg taaccagttt aatgaaagaa aaaatcatcc
    12421 aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta
    12481 tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat
    12541 agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt
    12601 ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga
    12661 gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga
    12721 ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat
    12781 agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga
    12841 gcatatctgc tattactgtg attttaataa agttttttta gaagggcagc cagtagatga
    12901 gtatgtggaa atgctgctga atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa
    12961 tttagaaaca ctttacgtag ggggcgggac accaacttct ttatctgcta aacaacttga
    13021 tcgactgtta tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt
    13081 agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt
    13141 caatcgttta tccatgggag tgcagacatt tgataatcgc ttattgaaaa aaattggacg
    13201 taaacacacc gcagaagatg tgtatcaaac tatgcaattt ttagaagcag aaaattttac
    13261 caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag gatatctgga
    13321 tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct
    13381 cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca
    13441 ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca
    13501 ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta
    13561 ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat
    13621 tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg
    13681 cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt
    13741 aggactaaga aaaaaaacag ggattactaa aaaacatttc tatgatcgct atcaaaaaac
    13801 aattgaatcg gtgtacggtg atgtgctaag ggaattagag caagaagggt tacttgttaa
    13861 tgaagaagac cgtgtttatt tgacaccaag agggaccttt ttggggaaca atgtgtttga
    13921 gcgctttttg tttgaaaaat agtttaaaga atatttgttt tttatctgaa tttaaataaa
    13981 aaaagttcaa aaatacaata aaggactatt gaaacgttga tataactgga tttacatcaa
    14041 ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca
    14101 ggagaagggc ttcttgctat atttataagt ggattagcac ttagaatata tgagtgctaa
    14161 tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa
    14221 cactatacga gtagtggcgt gcccgttggt tccaaaacat tgatgtctga gggagtcaag
    14281 gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg
    14341 aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat
    14401 catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta
    14461 ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag
    14521 ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc
    14581 tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg
    14641 aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa
    14701 atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa
    14761 cttcgtacgg agattccgat gattcttcat aaatatttcc aaacgacaga agggatgtct
    14821 cagttatttg acaccgttct tggtcatgtt tttgaagata aggtttatgt cagtggacaa
    14881 atgaatctct tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct
    14941 tttatgaaag actcagacgg acttacacaa atgatcactc cgatggatag caatattcat
    15001 atcaagattg gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca
    15061 agttacgaga tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg
    15121 ccttattctc ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt
    15181 gctgattact accgttcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg
    15241 cagctgatcg ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag
    15301 gaagactgta tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag
    15361 ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg
    15421 gaagaattcg aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg
    15481 cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta
    15541 caaaaatacc gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa
    15601 cgtgcattag ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa
    15661 atggtcttgg aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct
    15721 aaaggagaag cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa
    15781 gatacaccag ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc
    15841 gtgttaagac caaccatggt cattgttgca caataattgt gcggatataa aacttacact
    15901 ttcaattaaa gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga
    15961 ctaactctgc agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag
    16021 gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag
    16081 ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg
    16141 gtgaagcagg ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag
    16201 caatgatcct tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa
    16261 aagcagttat cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg
    16321 ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc
    16381 ttgcctatgg tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg
    16441 gcggtacttt tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa
    16501 cagctggcga caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg
    16561 tagcagaatt caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac
    16621 gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa
    16681 tcagcttgcc atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga
    16741 cacgtgcaaa atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac
    16801 gtcaagcgct aaaagatgca ggtttatcac aatcagaaat cgacgaagtt attttagtcg
    16861 gtggatctac tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac
    16921 caaacaaatc agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg
    16981 tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta
    17041 tcgaaacaat gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa
    17101 gtaaatcaca agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat
    17161 tacaaggtga acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag
    17221 atattccagc agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa
    17281 acgggattgt aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga
    17341 tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg tatggtaaaa gatgcagaag
    17401 caaatgcaga agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagatg
    17461 cattgctatt ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag
    17521 aagtgaaaaa agcagaaaca gctcgcgatg aattgaaagc cgctgttgaa gcaaacaaca
    17581 tcgaagaaat gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta
    17641 aactatacga acaagcggca caacaacaag ctcaagaaaa cccagaagca gctcaaggcg
    17701 gagcagatga tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag
    17761 ttcactgaat ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag
    17821 tttgaagtat cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat
    17881 gatgagaacc aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag
    17941 ggctctcaaa aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa
    18001 aataccatcc tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag
    18061 aagcatatga gattttaagt gacccgcaaa aacgcgctgc atatgaccaa tacgggcatg
    18121 caggcacaga tccaaattac ggtgctggtg gagctggtgg cggatttggt ggattcggcg
    18181 gcttctctgg tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag
    18241 gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg
    18301 acttgtcttt tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag
    18361 aaatctgtca tacttgtggt gggaacggtg caaaaccagg cacacaacca actacttgtc
    18421 ataaatgtca tggtgcaggt acgattaatg tagaacgcca aacaccactt ggacgagtga
    18481 tgagccgtca aacttgtgat gtttgtcatg ggacagggaa agaaatcaaa gaaccttgtc
    18541 caacctgtca tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag
    18601 ctggtgtaga agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg
    18661 gcggaccatt tggtgatttg tatgtggtct tccgtgtaga agaaagtgat atctttgatc
    18721 gtgaaggttc tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg
    18781 atgaagtcca agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac
    18841 agacaggtac gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa
    18901 caggcgacca acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa
    18961 gagaagcatt acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag
    19021 acggtttctt cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct
    19081 agtaaaaacg ctaagtcatt aataattaaa aaccagacga acaatcatat gttcgcctgg
    19141 tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat atgataatcg
    19201 tcatagtttc taggggtata accagtgaga gaatggaact tgttcaatcg atattgcaag
    19261 gtgtttttat gaataaataa ttcttctgag caatttttga tgcttccgtt atttttactg
    19321 aagcatgcca aagtcttctt gaacaaatgt acttcttctt ctgtcaggct tttcaaaata
    19381 cgttttacat ataatgttcg ttgatctgac gggattgctg gtaaaacgat cccgaggtct
    19441 aaatcttcaa aaaagcggat ctcgttcaca gattgatgga aagctatcca ttcgcaaact
    19501 tgtttggcat gaaggaaaga ttgcggtgtc tcttgctctg tataacaaag cgacccaatt
    19561 ccaaagttta gctgtaactg atacatttta ttaaactttg tctggatttt atcaagcaat
    19621 gatagcaatg aggctctgga aatccgattg actaggataa tgatttgttg tctatgtaca
    19681 gtgagcaaag cctgtccgta ataagagaag accctttcga gcaactccaa taaaggattg
    19741 atccgttctt gacacaattg ctgattgtca gaaagttgtc ccacaactac ataatatgga
    19801 gaagaaaaat caagctcaat taagtgaggg tggtatcgac tatgtacttg atatcatcaa
    19861 tgaaccgatg gtactttctt atgcgatgga acaggtagag cgattgcttg tttgtgcagg
    19921 aaaacaagcg atgcggctga tacgattagg tgagaaaata agaagataaa aatacacggt
    19981 cccttcatgg cgaccgtgca tttttggttt ttattaaata tttaatactt tattcaagaa
    20041 atcttttgtc cgttcatttt gcggattttc aaagatttgt tgaggcgttc catcttctaa
    20101 aaagtttccg tcgtcaatga acatgacgcg attggccact tctttagcga accccatttc
    20161 gtgcgtaacg ataaccatcg tcataccttg ttctgctaac tttttcatga cgttcaagac
    20221 atcaccgacc atttcaggat cgagtgcaga agtaggctca tcaaaaagca tgatgtcagg
    20281 gttcatcgct agagcacgag caatagccac acgctgtttt tgtccaccag aaagcatttc
    20341 aggatacata tcttttttat cttctagacc aactgtctgc agtaatttga ttgctttttt
    20401 ctctgcttct tctctagatt ctttcttcag gtccatcggt gctaaaatga tgttttccaa
    20461 aatagaaaga tgagggaata ggttaaagtg ttggaatacc attccgatat gttgtctcac
    20521 caagttgata tctgtgcttt tatccatcaa gtgggcgccg tcaataataa tttcaccgtc
    20581 agaaggttct tctaaacgat tcaagcaacg caagaatgta ctttttcctg aacctgaagg
    20641 gccaatgata cagacgacat ctccttcgtt gatggataca ttgatgtcgt ttaatactgt
    20701 gttatcgccg tattttttga ctaaatgctc aactaagatt ttttctgcca tcttacttca
    20761 ccttcttttc taaaactttt gctaattttg tcaatgctgt gatcaaaatc aagtacatga
    20821 tcgcaatgat caagtaaaca tacgtacttt gtgttgtacg tgcaacaatg atcttaccag
    20881 tttgcagcaa ttcgactaca ccgattgcgg aaatgatcgt tgtgtctttt aatgagataa
    20941 cgaactgatt gacgaatgat gggatcatga tgcgtatcgc ttgcggcaaa atgatttttt
    21001 gcattgtccg attataagaa agacctaaac ttctggaagc ttccatttgt ccgacaggaa
    21061 cggcgttgat cccgcctcga acaatctctg cgatataagc acttgcgtta agagtcaagg
    21121 tgataacacc agcaaggaag tcaggaatcg taaatccaac gataccaggc aacccaaaga
    21181 aaataaagaa cgcaagaacc atcattggaa ttcctcggat gatatcgaca taaatactag
    21241 caattgtgcg aagagcgcgt attggtgcta cactgaataa tccaaaaatc acaccgatta
    21301 ctatagccaa agcaaaggag atcaaagtta ggagaattgt tctccaaagt cctttcaaca
    21361 atgcttgata gttgttttgg ataagtccaa gaaatgttga ttcatcagca gtggaagact
    21421 cactggaagt ttctgaacca tccgctacat attctttaat aatcttatcg tattcgcctg
    21481 ttctttttaa ttctttcaat ccttcattga acatattaag aagctcagga ttttgtcctt
    21541 ttttaacggc aaatgcatat tttccgccag attctttttt gatcggtgtt tccaattctt
    21601 gtccttgttt gatcgcataa ccgattaccg gataatcatc catcattgca tcgattgcgc
    21661 caacttttaa cccgtcgtac aattgatcag cagcatcaaa atatttgata gaatagccat
    21721 attcagattc atggtcgctt aaaaattcag cactttcggt accaactttt gctccgactg
    21781 tttttccttt caaatcttca taagacttga tcgaagtatc tccttttttg acagctaatt
    21841 ggatcccgct atcaaagtaa gaatcagaaa aatcatagct tttctttcgt tcatctgtta
    21901 cagtcattcc ggcaatcatt ccgtctgctt gtcctgattc caaagcttgc aaagcagcag
    21961 agaaaccacg gtagtccatt cggatgttaa atccttgcag tttggcgatg ttttccatca
    22021 attttacatc gatgccttcg tactcgccct ttgcgttttg aaattcaaat ggagcaaaag
    22081 cagagtcact ggcaattacg tatacgtctt ttttgggctc tattttttgc attgtctcgc
    22141 ttgtgcttga ctctgccgtt gtagtgttgt cagcttgtgc aacatgacca aacgcaagtg
    22201 tcacaagtcc tgtcattaag aaaacaaatg agtgaaataa atgctttctt ttcataatca
    22261 acctcctaaa tttccaatat tttattatta taggcgaaaa gtgctgaatc agacaagact
    22321 gtaagctcga aaaaatgaaa aaaatgcgag tttctaacat tttatgttat aaaacataac
    22381 atgtataata agataaaaaa gtggcgaaag tatgttcggt gtggtataat tttttagtaa
    22441 ggaatgcctt tgctttcctt ttctttttat tggtcgtaaa atgaaaagga atgaatgaaa
    22501 aggaggacga agaatgattg agagagatac ctcccgacac tttgagcttg tctcgaaata
    22561 ccaaccggct ggtgatcagc ctgaagcgat taaccaatta gtggatggcg tagtcggcgg
    22621 gaaaaaagca caaatcttat taggtgcgac tggaacaggg aaaacatata cgatttccaa
    22681 tcttattgag aaagtgaata aaccaacact gattattgcc cataacaaaa cattggctgg
    22741 tcagctgtac ggggaattca aggaattttt ccctaacaat gctgttgaat attttgtttc
    22801 ttattatgat tattatcagc ctgaagctta tgtgccttca agcgatacgt atatcgaaaa
    22861 agattcaagt gtaaatgatg agatcgacaa attacgacat tcggctacta gttcattatt
    22921 agaaagaaat gatgtgatcg tcattgcatc cgtttcatgt atttttggat taggctcgcc
    22981 ttttgagtac caaaaacaag tagtttcgat tcgacaggga gcagaactag atcgcaatca
    23041 gttgattcgt gacttagtca gtatccaatt cgaaagaaat gatatagatt ttcagcgcgg
    23101 tcgttttcga gtgcgcggtg atgtcgtgga gatttttccg gcttcaagag atgaacgtgc
    23161 cttacgtgtg gagttttttg gagatgaggt cgaacgtatc cgagaagtca atgcactgac
    23221 aggtgaagtt ttaggagaaa cggaacacgt agcaatcttc ccggcgactc actttgtgac
    23281 gaatgatgaa catatggagc atgccgtagc gaatatcaaa gaggaattag aacagcgttt
    23341 aactgtcttg cgaaatgaaa acaaacttct ggaagctcaa cgtttagaac agcgaaccaa
    23401 ttacgatatt gaaatgatgt tagaaatggg ctacacgtcg ggaattgaaa attattctcg
    23461 gcatatggac ggacgtaaag aaggtgaacc gccttatacg ctattggatt tctttcctga
    23521 agattttttg atcgtggcgg acgaatcgca cgtaaccatg cctcaaatca gaggaatgta
    23581 taatggtgac cgagcgagaa aacagatgct agtagattac ggtttccgtt tgccatctgc
    23641 tttagacaat cggccacttc ggttagaaga gtttgaaaaa catgtcaatc agatcattta
    23701 tgtctctgcg acgccaggac catatgaaca tgaacaaaca gataccgtta tccaacaaat
    23761 catccgtccg acagggctgc ttgacccagt gatcgaagtt cgtccaatca tgggacaaat
    23821 cgatgatcta gtaggcgaaa taaatgaacg agtcgaaaaa gaccagcggg tgtttgtaac
    23881 gacattgacg aaaaaaatgg cagaagacct cacagattat ttcaaagaat tagggatcaa
    23941 agtcaaatat ttgcatagtg atatcaaaac gctggaacga acagaaatca ttcgagatct
    24001 tcgattaggt gaatttgatg tgttagttgg aatcaatttg cttcgtgaag gattagacgt
    24061 acctgaagtt tcccttgttg ctatcttgga tgcggataaa gaaggctttt tacgtagtga
    24121 acgttcctta gtccaaacga ttggacgagc tgctcggaat gaagaaggta aagtaatcat
    24181 gtacgcagac aaagtaacag attctatgcg tctagcgatg gatgaaacgt caagacgtcg
    24241 tacgattcag cagaagtaca atgaagaaca tgggatcgtt ccaaaaacga tcattaaaga
    24301 aatccgagat ttgatttcta ttacgaaaga atcggaagat gatacaaaag aagcggtaca
    24361 agtctcgtac gaagaaatga cgaaggaaga aaaagacacg ttattgatga aattagaaaa
    24421 agaaatgaaa gatgcggcaa aagctttgga ttttgaaaca gccgcgaatg tgcgagatat
    24481 gattttagaa ttaaaagctt caaagtagga ggacaacatg gctaacgata aaattacgat
    24541 tcatggtgca cgtgcccata acttgaaaaa tatagatgta acgattccac gagataaatt
    24601 cgtggtcgtt acaggtttgt ccggttctgg aaaaagttcc ctagcatttg atacgctgta
    24661 cgctgaaggg cagcggagat acgtagagag cctttctgct tatgctcgtc agtttttagg
    24721 acaaatggat aagccggacg tagatagcat cgacggattg agtccggcga tttctattga
    24781 tcagaaaaca acaagtaaaa atcctcggtc gacagtaggt actgtgacgg aaatcaatga
    24841 ctatttacgc ttgttgtttg cacgtgtagg acatccgatt tgtcccaacg atcatatcga
    24901 gattaccagt caatcagtag atcaaatggc agatcaagta atggctttgc ctgagcgcac
    24961 aaagatccaa gtgttggcac cgatcgtggt aaagaaaaaa ggacaacata aaaaaatatt
    25021 cgaacgaatc caaaaagagg gatacgtccg cgttcgagtc gatggtgaga tctatgattt
    25081 aagtgaagtg cctgagctgg agaaaaacaa aaaacatgat atcgcgatcg tcatcgaccg
    25141 gatcattgtg aaagaaggga ttcgttctcg cctatttgat tctttagagg cggccctgcg
    25201 attagcagac ggctatgctc tggttgacgt gatcggtgaa gaagaaatgt tgtttagcga
    25261 acactatgcc tgtccttatt gcggatttac tgttggcgaa ctagaaccaa gactgttctc
    25321 attcaatgcg ccttttggag cttgtccaga ctgtgatggt ttaggtgtca aattagaagt
    25381 cgatcaagat ttagtgattc cagatagaac gaagacactt agtcaaggtg ctatcgttcc
    25441 atggaatcca atcagctctc agtattatcc tcaaatgtta gcgcaggctt gtgaaagttt
    25501 cggtatcgat ttagatacac cattcgaaga attaccggaa gagcatcagg agatcatctt
    25561 gaatgggaca gatgatcttt tccatttcca ttatgaaaat gattttggtg gcgttcgcga
    25621 tgtagaaacg actttcgaag gggtattgaa gaatatcgag cgtcgttatc atgatacgaa
    25681 cagtgatttc acgcgtgagc aaatgcgtct ttatatgaca gaattaactt gtcagacatg
    25741 taaaggttat cgattgaatc cccaagctct atctgttcaa atcaatggaa cgaatatcgg
    25801 gcaaacaaat gagttgtcta ttcaaaaagc agtagatttc ttctcaaact taacgttatc
    25861 tgaacaagag aaagtaattg ccaaaccgat tttaaaagaa gtaaatgaca gactttcatt
    25921 cttggaaaat gtgggactag actatctaac tttgagtcgc gcttccggaa cattatctgg
    25981 gggagaagcg cagcgaatca gattggctac acaaattgga tcgaatcttt ctggagtttt
    26041 gtatatttta gatgaaccat cgatcggatt gcatcaacgc gacaacgatc gactgattgg
    26101 ctcattaaag aaaatgcgtg atcttggcaa caccctcgtt gtagtcgaac atgatgaaga
    26161 tacgatgtgg gcagcagatt atttgattga cgtcggacct ggggccggag aacaaggcgg
    26221 acaaatcgtg gctgcaggca ctcctgaaga agtagctaat gatgaaaatt ctttgacagg
    26281 gaaatatctt tcagggaaaa aatcaatccc tgtaccaaaa gaacgccgta aaggtaatgg
    26341 gaaagcaatc aagatcactg gtgcttcaga aaacaatctg aaaaatatta gtgttgaatt
    26401 tccattagga gaatttgtag cagtcacagg tgtgtctggt tctggaaaga gtacattggt
    26461 caatagtatc ttaaagaaat cgttagcgca aaaattaaat aagaattctg ctaagccagg
    26521 taaattcaag acaatttccg gctacgaaag tatcgaaaag atcatcgata tcgatcaaag
    26581 cccaatcggc cggacgccga gaagtaatcc agcgacttat acaagtgtat ttgatgatat
    26641 ccgtgggtta tttgctcaaa cgaacgaggc aaaaatgcgg ggttataaga aagggcgttt
    26701 tagtttcaac gtaaaaggcg gtcgttgtga agcttgtcgc ggggatggaa ttattaagat
    26761 cgaaatgcac tttttgcctg atgtctatgt tccttgtgaa gtatgtcatg gtaaacgata
    26821 taattctgaa acattagaag tgcattacaa aggaaaaagc attgctgata ttttggaaat
    26881 gacagtagag gatgctgtag aattcttcaa gcacattcca aagattcatc gcaaactgca
    26941 aacgattgtt gatgttggct taggttatgt gacgatgggg caaccagcaa cgacattgtc
    27001 cggtggtgag gcacaacgga tgaaacttgc cagtgaattg cacaaaatct ctaatggaaa
    27061 gaatttctat atactagatg aaccaacgac aggacttcat agcgatgaca tcgcccgctt
    27121 gttgcatgta ttacaaagat tagtagatgc tggtaacaca gttttagtga ttgaacacaa
    27181 tctagatgta atcaaaacag cagattatat cattgattta ggaccagaag gtggagaagg
    27241 tggaggaacg atccttgcga ctggaacacc agaagaaatc attaacgtaa aagaaagtta
    27301 tacaggtcac tatttgaaaa aaataatggt ataaagagga tagatagctg agatagttaa
    27361 aaaaacataa aaaaatgaat ttttgcttgt tttattaata tcagtgttct agaatcatta
    27421 catggtagtt ctttctacac tgattggtat agataaaaac ttcctataag ttcaatccgt
    27481 tcaaaaaaga attgagcaat cggaggactt tcctttgatt gcttttttct ttatggtatg
    27541 atattccttg tgtagttgga aaagaaaaac aagagattat gagtaagtga gagataatga
    27601 tgaacagtat tgaaaatgaa gaatttatcc ctatattatt aggaagcgac atgaacgttt
    27661 atggtatggc acgttctttt aatgaagcat acggcaaaat ctgccaagca tatgcttcgg
    27721 atcagttagc ccctacccgc tacagtaaaa ttgtcaatgt agaagttatt ccaggatttg
    27781 ataaagaccc tgtatttatc gagacgatgc ttcgtttggc aaaagaaaga tattctgata
    27841 agagtaaaaa atatcttttg attgcttgtg gtgatggata tgcagaattg atctcccaac
    27901 ataaacaaga gctgtcagaa tattttatct gtccatatat tgattattct ttatttgaaa
    27961 ggctgatcaa taaagtaagc ttttatgaag tttgtgaaga atatgatctt ccgtatccta
    28021 aaacattgat cgtcagagaa gaaatgttag taaatggaca tttggaacag gaattgcctt
    28081 ttgaattccc agttgctttg aaacctgcaa acagtgtgga gtatctttct gtccaatttg
    28141 aaggaagaaa aaaagctttt attttagaga ctagagaaga gttcgattta attttaggcc
    28201 ggatatacga agccggttat aaaagtgaaa tgattgtaca agattttatc ccgggtgatg
    28261 atagtaatat gcgtgtgcta aatgcgtatg tagatgaaga tcatcaagtt cggatgatgt
    28321 gccttggtca tccactatta gaagatccta caccagcatc gattggtaat tacgttgtga
    28381 tcatgccaga ttataacgaa aaaatctatc aaaccattaa ggcatttttg gaaaaaatcg
    28441 aatatacagg ctttgccaat ttcgatatga agtatgatcc tagagatgga gaatataaat
    28501 tatttgaaat caatttgaga caaggtcgta gcagtttctt tgtgacattg aacggcttga
    28561 atctggctcg ttttgtcaca gaagatcgag tgttcaacaa accctttgtt gaaacgacct
    28621 atggaacgaa tcaatcagat aaagcaagat tatggatggg tgtgcccaaa aaaatctttt
    28681 tagaatatgc tcgggaaaat gaagacaaaa aactagctga gcaaatgatc aaagaaaatc
    28741 gttacggtac tactgtattt tatgaaaaag accgatcaat caagcgctgg ttgttgatga
    28801 aatatatgtt ccataactat attccaagat tcaaaaagta ttttcatgtg aaagaaggct
    28861 agttcatgga gaattttttc agtattttag gcggaatggg cacgatggcg acagaaagtt
    28921 ttgttcgctt gatcaaccac cgaacgaaag ctacaaaaga tcaagaatat ttgaattatg
    28981 tcttattcaa tcatgcaaca gttcctgatc gtacagctta tattttagat cgatcagaag
    29041 aaaatccaat gccgtttctg ctggatgata ttgagaaaca aaatttattg cgaccaaact
    29101 tcattgtgtt gacttgtaac accgcacact acttttttga agagttacaa gcggcaacgg
    29161 atattcctat cttgcatatg ccaagagaag cagcaaatga acttgtccgt cagcatacga
    29221 cagggagagt agcgattcta ggaacagaag gaagtatgaa agctggaatc tatgaacgcg
    29281 aagtcaaaaa tcttggtttc gaaacagtga ttcctgatac tgcgcttcaa gaaaaaatca
    29341 attacctgat ctatcatgag atcaaggaat cggattattt gaatcaagag ctctattacg
    29401 aaatcttaga ggaagcggta gagcgcctaa actgtgaaaa agtcatccta ggttgcacag
    29461 aattatcttt gatgcatgaa tttgctgaag ataatcatta cccagtgatc gatgctcaat
    29521 ctattttagc ggatcgaacg atcgaacgtg cattagctga aagaagcgag gcattggata
    29581 cagcatcgga aaagtaaaaa aattggattt ggggcaaata tcctaaaaga tatttggtcc
    29641 aaatcttttt gtaaaagaaa aagaaaactg gtaatgatac gcccgggatg atataatttt
    29701 attatgcaca atgtgagagg ggttcgtaaa tatgacagat aatttgcaat tagtcatcat
    29761 aacaggtatg agtggtgcag ggaaaacagt agctatccaa agttttgaag atatgggcta
    29821 tttttgtatc gataatatgc cgcctcgatt gattccaaaa ttctgggaat tgatcaaaga
    29881 atcaggtaaa gtcacaaaaa tggctttagg agtggatttg cgttctcgtt ccttctttga
    29941 agaaatccaa aacatgctga tcgaaatcga aaatacgaac ttcatcgaca cttctatctt
    30001 atttttagat gcttcagacg aagaactagt ggctcgctac aaagaaacaa gacgagcaca
    30061 tccaatggcg atggacggtt tgatcactga agggatacgt aaagaacggg cgatactaga
    30121 cgacttgaaa gcccaagctt ccgtgatcat tgatacgaca acattgacac cacgacaatt
    30181 acgtgaaaaa atcaatgagt cattcaaatc aaagggagac caaggatttc ggatcgaatt
    30241 tgtatcattt ggttttaaat acgggttgcc gatcgattcg gatattgtga tggatgtgcg
    30301 ttttttgccc aaccctcatt atatcccgga actaagacca ttgaccggaa aagatcagcc
    30361 agtctatgat tatgtgatgt cctttgatga aacgcaatca ttctaccata aatttttgga
    30421 tcttctggaa actgtattgc ctggatacat taaagaagga aaaagcagct tgacagttgc
    30481 aatcggctgt acaggcggtc aacaccgttc tgtggcattg actgaacgtt tggcaaatgc
    30541 tttagggaaa aactataaag tcaatgtgac acatcgggac aaggataaaa ggaaagagac
    30601 ggtgaaccgg tcgtgaaaat gaaaacctac cggataagaa aaccaaagat cgtagtggtc
    30661 ggcggtggca ctggtctgcc agttatcctg aaaagtttac gaaatcaagg agcagatatc
    30721 acagcagtag tgactgttgc agatgatggt ggaagcagcg gtgcaatcag agaatcgatc
    30781 gctatggctc ctcctggtga cttgagaaat gtcttagtgg cgttatcgga tatgccacaa
    30841 ttctatgaag atattttcca ataccgtttc aaaaaggaag atcagttttt agccaatcat
    30901 acgatcggta atctgatcat tgccgcagtt tccgagatgc ggggaagcac ttatgaggcg
    30961 attcaattat tagcaaaaat gatgcatgta gatggacatg tttatccttc ttcagaaact
    31021 cctttgacct tgcatgctgt ttttaaagac ggaacgactg cagtaggaga atctaaaatc
    31081 gcgatcaacc gtaaaaccat tgaccgtgtg tatgtctgca acacaaatga taaaagtgaa
    31141 gccaaagcgg ctcgaaaagt cgtttcagca attatggacg ccgacatggt cgttttggga
    31201 cccggaagtt tattcaccag tatccttcca aatttagtga taccagaaat cggcgaagca
    31261 atgctcaaga caactgctga aaccgtctat atctgtaata tcatgaccca aaaaggggaa
    31321 acggaacatt tcacagatgc cgatcatata cgtgttttac acaagcattt gaatcagcct
    31381 tttatcgata cagtattggt caatacggaa aaagtgcctg aagattatat ggacccagag
    31441 atctatgatg agtatctagt acaagtcgca cacgacttca acggattgag aaatgaagga
    31501 tgtcgagtga tttccaccga ctttttagaa ttgaaagatg gtggtgtctt ccacgatgga
    31561 gacaaagtag tagaagaatt gttccgttta gttttcggtt caaaatatta agcaatttat
    31621 tgttggagaa aggaggagaa taggatgtca tttgctgcag atgtgaagaa agaactaact
    31681 ggtttggaag ttcatcgaga acatgcgaaa gcggaattag ctgctttgct gcgaatgaac
    31741 ggctctttag gtattgtgaa ccagcagttt attttgaacg tccaaacaga aaatgctgca
    31801 attgccagac gtatctattc tcttttgaaa gatcattatc aggtacgaag tgagctactc
    31861 gttcgtaaaa aaatgaaatt aaaaaagaac aatgtctata tcgtccggtt gaagcaaggc
    31921 acaaaagatg tgttgatgga tcttgatatc atggatggta tgatgttcaa tggacacgtt
    31981 tcggacgaga ttatgggaaa ttcgcaaaaa atgcgttctt acttacgtgg tgcttttatg
    32041 gcctcaggtt cagtcaataa tccggaaacg agtcgttatc atttggaaat attttctatt
    32101 tatgaacagc ataaccaaga catttgcgat atgttaaatt attacggtct acatgctcga
    32161 acgcttgaac ggcgcaatgg ctatatttcg tatttaaaag gagcagaaaa aattgctgat
    32221 ttcttaacat tgattggtgc aaccaactca atgctccgat ttgaagatgt tcgaatcgta
    32281 cgggatatga gaaattcggt caatcgtcta gtcaattgtg agacagcaaa tctgaacaag
    32341 acgattgatg cggcttctaa acaaatcgaa aacatcgaat tgatcgaagc aaaggtcggt
    32401 ctacatgccc ttcctgaaaa acttcaggaa atcgcagaac ttcggcttca acatccagag
    32461 gtcagcttga aagaattagg ggaaatgatt ccctcaggtg caatatcaaa atcgggaatt
    32521 aatcaccgaa ttcgcaaaat aaatgaattt gcggaaaaac tgcgtgaaga agcagtttaa
    32581 tccaaagaaa aa
//
LOCUS       DACCCS010000030        30947 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.030, whole genome shotgun sequence.
ACCESSION   DACCCS010000030 DACCCS010000000
VERSION     DACCCS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.030"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            162..1103
                     /locus_tag="IVK82_001440"
     CDS             162..1103
                     /locus_tag="IVK82_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP6392511.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1172..1903
                     /locus_tag="IVK82_001441"
     CDS             1172..1903
                     /locus_tag="IVK82_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP6392512.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2047..4227
                     /locus_tag="IVK82_001442"
     CDS             2047..4227
                     /locus_tag="IVK82_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP6392513.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4247..4729
                     /locus_tag="IVK82_001443"
     CDS             4247..4729
                     /locus_tag="IVK82_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP6392514.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4745..5506
                     /locus_tag="IVK82_001444"
     CDS             4745..5506
                     /locus_tag="IVK82_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP6392515.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5637..6971
                     /locus_tag="IVK82_001445"
     CDS             5637..6971
                     /locus_tag="IVK82_001445"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP6392516.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7278..7763
                     /locus_tag="IVK82_001446"
     CDS             7278..7763
                     /locus_tag="IVK82_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP6392517.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7756..8439
                     /locus_tag="IVK82_001447"
     CDS             7756..8439
                     /locus_tag="IVK82_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP6392518.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8565..11210
                     /gene="polA"
                     /locus_tag="IVK82_001448"
     CDS             8565..11210
                     /gene="polA"
                     /locus_tag="IVK82_001448"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP6392519.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11255..12091
                     /gene="mutM"
                     /locus_tag="IVK82_001449"
     CDS             11255..12091
                     /gene="mutM"
                     /locus_tag="IVK82_001449"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP6392520.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12055..12684
                     /locus_tag="IVK82_001450"
     CDS             12055..12684
                     /locus_tag="IVK82_001450"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP6392521.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            12736..13068
                     /locus_tag="IVK82_001451"
     CDS             12736..13068
                     /locus_tag="IVK82_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP6392522.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13380..13871
                     /gene="nrdR"
                     /locus_tag="IVK82_001452"
     CDS             13380..13871
                     /gene="nrdR"
                     /locus_tag="IVK82_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP6392523.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            13894..15267
                     /locus_tag="IVK82_001453"
     CDS             13894..15267
                     /locus_tag="IVK82_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP6392524.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15267..16193
                     /gene="dnaI"
                     /locus_tag="IVK82_001454"
     CDS             15267..16193
                     /gene="dnaI"
                     /locus_tag="IVK82_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP6392525.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16322..16762
                     /locus_tag="IVK82_001455"
     CDS             16322..16762
                     /locus_tag="IVK82_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP6392526.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            16906..17607
                     /locus_tag="IVK82_001456"
     CDS             16906..17607
                     /locus_tag="IVK82_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP6392527.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17604..18725
                     /locus_tag="IVK82_001457"
     CDS             17604..18725
                     /locus_tag="IVK82_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP6392528.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            18740..19972
                     /gene="pepT"
                     /locus_tag="IVK82_001458"
     CDS             18740..19972
                     /gene="pepT"
                     /locus_tag="IVK82_001458"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP6392529.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20104..21012
                     /locus_tag="IVK82_001459"
     CDS             20104..21012
                     /locus_tag="IVK82_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP6392530.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21047..21319
                     /locus_tag="IVK82_001460"
     CDS             21047..21319
                     /locus_tag="IVK82_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP6392531.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21330..22376
                     /locus_tag="IVK82_001461"
     CDS             21330..22376
                     /locus_tag="IVK82_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP6392532.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            22627..25236
                     /gene="clpB"
                     /locus_tag="IVK82_001462"
     CDS             22627..25236
                     /gene="clpB"
                     /locus_tag="IVK82_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP6392533.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25387..26058)
                     /locus_tag="IVK82_001463"
     CDS             complement(25387..26058)
                     /locus_tag="IVK82_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392534.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(26051..26545)
                     /locus_tag="IVK82_001464"
     CDS             complement(26051..26545)
                     /locus_tag="IVK82_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP6392535.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26565..27785)
                     /locus_tag="IVK82_001465"
     CDS             complement(26565..27785)
                     /locus_tag="IVK82_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6392536.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            27932..29767
                     /gene="lepA"
                     /locus_tag="IVK82_001466"
     CDS             27932..29767
                     /gene="lepA"
                     /locus_tag="IVK82_001466"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP6392537.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(29861..30943)
                     /locus_tag="IVK82_001467"
     CDS             complement(29861..30943)
                     /locus_tag="IVK82_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6392538.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10303 a         5542 c         6629 g         8473 t
ORIGIN      
        1 ttttctgtgc gaaacaggaa agcgaagtct ttttaattca aataataata ttatgtaaac
       61 aaaaaacttt ccatgcgtaa ttatacacaa gcaaataaat cgtcaaatgc gaacgccttc
      121 tttatactgt ttaggaacga aaagaaggaa ggaagaaata catggattat caagtactgc
      181 tttattacaa ttatacaaaa atcgaagaac cagaggaatt tgccaaaaat catttagcct
      241 tttgcaaatc tttggatctt aaaggtcgta ttctagtagc tgaagaagga ataaatggta
      301 ctgtgtctgg aaccgttgaa aacacgactg cctatatgaa agcaatgaaa gctgatcctc
      361 gatttgctga tacgatgttc aagatcgatc caagcgaagg acatgccttt aagaagatgt
      421 ttgtaagata ccgtccagag cttgtaagtt taaagctaga agaggacatt gatccaaacc
      481 aagtgacagg tgcctatctt tcaccgcaag aatttaaaga agctatttta gatgaacaga
      541 cagtggtgat tgatgcacgg aatgattatg aatatgactt gggacatttt cgaggggctg
      601 tacgcccaga tatacgcagt tttcgagagc ttccagactg gatccgcgag cacaaagaag
      661 aatttatgga aaaacgggta gtgacctact gtactggtgg cattcgttgc gagaagtttt
      721 ctggttggct tgtccgtgaa ggattcaaag atgtcggaca gctgcatggc ggtattgcta
      781 cttatggtaa ggatcctgaa gtccaaggag aattatggga cggcaagatg tatgtgtttg
      841 atgaacggat cgctgtcgat atcaaccaag tagataaaca aatcgtcggt cgcgactggt
      901 ttgacggaac accatgtgaa cgatatatca attgtagcaa tcctgagtgc aatcgtcaga
      961 ttcttgcttc cattgaaaat gaagaaaaac atttaggagc atgtagcata gagtgtgcga
     1021 aacatccgcg caatcgctat gttcaagacc atggaatctc tccagaagaa gcagaactgc
     1081 ggtttacagc tttaattaaa tagaaaacaa tagttttgat tgtcgaccat ttccaatatg
     1141 cgctatacta gcgttgagga tggtgatcca catgaaatca ttgatttatg ctattgggga
     1201 tgtccatggc aaacatgagc tttttgaaaa acttattcac tattatgatc caaagatcca
     1261 tcagcttgtt ttattagggg atctcaatga tcgaggtcct cgtacaaaag aatgtttttt
     1321 attaggaaag caattagtcg aagaaacagg agccatttac ctccggggaa accatgaaga
     1381 atattttttg caatttctgc aatcaccgga ggactggtat gcgccttata tgagaaatgg
     1441 tggaaaagaa acgtttgaaa gcctgattca cccaggggca tcggaggaat actccccaac
     1501 tgaaatctcg atgatgatcc aatcgagata ttcagaactg attaaatttt tagtccagcg
     1561 ccccttgtat tatgaatggg aaaattatct ttttgtccat gccggtgtgg atttgacaaa
     1621 gaaagactgg cgtaagacaa gtctaagaga ctttatttgg ataagggaac catttcatga
     1681 agggaaaaac aatacaggta agacaattgt atttggacat acgattacac caatgctcca
     1741 tggagatatg cagacaacag acctctggat tcaggatcat aaaatcggaa tggacggtgg
     1801 ggctgtattc ggagggtcat tgcatggggt tgtttttgac aaggaaggta ttcttcaaga
     1861 tattgaatat cagaacaacg aaggaccttg gcatggaatc tgactaatgg aaagaatgaa
     1921 ggagctgtga ctttgttgcg gctcttttta tttttataga gaatattgtt tctttcagta
     1981 gtttaaccca ggataaaaag tatgatagaa taaagaagca atttttagca gaaaagaggc
     2041 attttaatga cggaaaattt gaaccagacg atcattcagc ttgtacaaaa agaactttca
     2101 gactatcgtc ctaagcagtt gactactgta cttaatttgt taaatgaagg aaacactgtt
     2161 ccttttatcg cgcgttaccg aaaagaaatg actggaagtc ttgatgaagt ccagattcgt
     2221 gaaatcgaag agcggtatgc ctatcttgaa aacttagaaa aacgtaaaac tgaagtgatt
     2281 cggctaatcg atgaacaagg gaaactgact cccgagttag aagcagaaat cacccaagcc
     2341 gtgaaaatgc aacaagtgga agatttgtat cgtccataca aacaaaaacg acggacaaaa
     2401 gcaacgattg ctaaagaaaa aggacttgaa ccactcgcga agtggctgat gcagcttaca
     2461 gatggagagg tgcaatctga agcagaaaaa tatattgaca aagaaaaaga agtctcttct
     2521 gccgaagaag cgttgcacgg tgctcatgag attattgctg aacaagtcag cgataatgca
     2581 aaatttcgga cttggattcg gtcttatacg tataacaaag gtatgtatgt cagcaatgtc
     2641 aaggatgagc aagcagatga aaaaggcgta tatgaaatgt attatgactt tgcagaaccc
     2701 gtccataaaa tggtttctca tcgaatatta gctactaatc gtggagaaaa agaagatatt
     2761 ctgaaagtat tctttcaagt agatgaagca gcaattttag cttacttgga tcgccaaata
     2821 gtcaaaaatc ctgcctctcc ttcttcttca ttcgtcagag aagcctatca ggatagttat
     2881 aaacgcttta ttcagccagc aatcgagcga gaactgcgca atgaattgac tgaaaaggca
     2941 gatgagcaag caatcgctat tttcggcgaa aacctccgta atcttttgtt gcaaccgcca
     3001 ctaaaaggta aagttgtcct gggatttgat ccggcttatc gtactggatg taaactagca
     3061 gtcgtagatg cgacagggaa agttttagca atcgaagtta tttatcctca taaacctgca
     3121 gcgcaagcaa aaagagaagc tgctggacca gcttttatcc aattaattaa taaatatcaa
     3181 gtagatatgg tagctatcgg aaatggtaca gccagtcggg aatctgaact ttttgtggct
     3241 gagcagttga aatccgccga ccataaaacc tattacgcta tcgtcaacga agcaggcgct
     3301 tctgtttact ccgctagcga aattgcacga aaagaatttc cgcatctaca agttgaagaa
     3361 agaagtgctg tttctattgc ccgtcgcctg caagatcctt tagctgaatt agtcaaaatc
     3421 gatcctaaag ctgtcggagt aggccagtac caacatgacg tttctcaaaa acgtttggct
     3481 gaacaattgg attttgttgt ggaaacagcg gttaaccaag taggtgtgga tgtgaataca
     3541 gcaagcccgc agctcttgca gcatatatca ggattgaata aaacaactgc ccaaaacatt
     3601 gtcatttatc gtgaagaaaa cggagaattc accgcacgaa ctcaattgaa aaaagtgcca
     3661 cgcttaggac caaaagcata tgaacaagca atcggattct tgcgagtccc tggtgggaaa
     3721 aacattttgg acaacacagg tatccatccc gaaagttatt ctattgcaaa agaaatatta
     3781 acttcagtcc aactttcaga aaaagagctt ggtacagaag aagcagtaga aaaattgaca
     3841 cgattatccg tagagaaact ggctgaaagc ctatcagtcg gagaagaaac gttagccgat
     3901 attttagctg ggctgacaca acctggacga gatatgcgtg atgagatgcc agcgccctta
     3961 ttacgtacag atgtattgag tatggaagac ttgaaaccag ggatggaatt gactggaacg
     4021 gtccgtaatg tcattgactt cggcgcattt gttgatatcg gtgtgaagca agacggactt
     4081 gtccatatat cgaaactcag caaaaaattc gtcaagcatc ctacagatgt cgtgtcagta
     4141 ggagatatcg taacagtttg gatagaacaa gtcgatacga aaaaaggcag aatcagtttg
     4201 acgatgcttt ccccttatga agagtaacaa tgaggagtaa caaaaattga cacaagaaga
     4261 actgcagcag ttagtagaag agatatcatt aaaaagcttc cagcttcctt tcaagcacca
     4321 agcaatgttc aatccacgct tacgtacaac tggcgggcgc tatcatttaa acgatcatca
     4381 tttagatttt aatgcgaagc tatttgaaga gtcagacgaa gagacgattg caggaatcat
     4441 caaacatgaa ctttgccatt atcatcttca tctagctgga agagggtatc agcataaaga
     4501 tatggatttc aagtatcttt tgaaaaaaac aggagggttg cgctatgcac cagccttgac
     4561 gaagaaacag ataaagatgg agtattatcg atgccaagat tgttctgcca cgatctgccg
     4621 caagagaaga atcgatgtac gcaaatatcg ttgtggaaga tgtcatggag aaatcaaatg
     4681 gattgagaca aaaagagaag actttaatga agcacaaaaa atcagatgaa aggatagaag
     4741 gcctttggaa aaaagtgagc agaggaatct aaaagactgg aatcagctat taaatttttt
     4801 agccgcacct gaagagggaa attttgagga actgacggca cctacactta ttttagctgg
     4861 aaactgtctt cctatcttag ctgataaagc agcacaaatg tatttaaatg gccaagtaga
     4921 tcaattgttt ttggtcggag gggtggggca tgcgactaga atcctttatg agaattttga
     4981 aaagcaagga tttcattttg aagatggcat gagtgaaagt gaaatctgtc gtcagtatct
     5041 aaaagaagtc tatgatttgc ccgataaggc ttttctgata gaatcgaagt ctacaaattc
     5101 tggagagaac gccacttttt cactggagat tcttcattcc ttaggagctg taccagaaaa
     5161 aatcttattg atgaatgatc cgacactgca acgcaggact cgagcaactt ttgagaaagt
     5221 atggcaaaat gaacaaacta ttttcgcgaa tgctgtgcca tttgtgccgg aaatccttca
     5281 cttttctgaa gagatcatct ttacagcaaa agaactgaat catcaatggc ctaaggaata
     5341 tttccatgcg ctagtattag gagaaatgga acgtctccat gacgatgaga acggctatgg
     5401 tccgaaagga aaagatttta ttcctcacat tgatatatca gaggaagttt ggtcttctta
     5461 caccagaatt aaagagtcaa taaaaacgga tttttctcgt acttgaccat tttagcaagc
     5521 gaatcctcct ttccaaataa atttggttga aattttcaat cgtttataag ataatgagga
     5581 gcgtgttgaa tgtaatagct aaaagaatgg acgctagata agagaagagg attcaaatgg
     5641 aaaatcaaaa taaattgtat cattttgttg gtatcaaagg ttcaggaatg agctccttgg
     5701 ctcttgtttt acatgaacaa ggtctaaatg tccaaggatc agacattgaa aaatatttct
     5761 ttacacaaag agatttagaa aaagcaaata tcacgattct gccatttaac gcagataatg
     5821 taaaaccagg gatgacgatc attgcaggaa atgcatttcc tgattcacat gaagaaatcc
     5881 agcgagcaaa agaattaggt ttggaagtga ttcgctatca cgactttatt ggtcatttta
     5941 tccaaaatta tacgagtatc gctgtaacag gctctcacgg aaaaacaagt acgactggac
     6001 tgctctctca tgtattgagt ggtgtccgtc caacaagtta tctaattgga gatggaacag
     6061 gacatggtga tccgcaagcg gaattctttt catttgaggc ctgtgaatat cgccgtcatt
     6121 tccttgccta ttcaccagac tatgcaatca tgaccaatat cgattttgat catccggatt
     6181 attatacaag tatcgacgat gtatttacag ctttccaaac aatggctgga caagtgaaaa
     6241 aagctatttt tgcgtatggt gacgatgctt atttacgcaa attaaaagca aatgtgccga
     6301 tttattatta tggtgtcaca gaaaacgatg atatccaagc acgaaacatc gaacgcacaa
     6361 cttctggttc tgcttttgat gtctatcatg gggatgaatt tgttgggcac ttcactgtac
     6421 cagcattcgg taaacacaat atcttaaatg ctttaggtgt tattgcagta gcttatttcg
     6481 aaaaactgga tctgaaagaa gttgcagaag aaatgctgac tttcccagga gtaaaacgtc
     6541 gattcagtga aaagatcgta gcagacatga ccgtagtcga tgattatgcg catcaccctg
     6601 cagagatcaa agcaacgatt gatggcgcac gccaaaaata tccagataaa gaaataattg
     6661 cagttttcca accccatacg tttacgcgta cgattgcatt gatggatgaa tttgcagaag
     6721 cacttgattt agctgataaa gtttacttat gcgatatttt tggttcggct cgggaagaac
     6781 aaggaaacgt taagattgaa gaccttggag cgaaaatcaa aaaaggtgga gaagtcatca
     6841 aagaaaataa tgtatctcca ttgttagatt atcatgatgc agtagtaatc ttcatgggtg
     6901 ctggagatgt tcaaaaattt gaacaagcgt atgaaaaact tttaagcagc acgacgaaaa
     6961 atgtactgta atacaattac tgcagtgtcc ttataaaaat agaaagaggg ggagtggcaa
     7021 aaatcttgcc aagccccctt ttttgcaact tatgataaac ggtgttcaaa agcttgcatc
     7081 tgcggtaata agcccaatca aatcaaaaac atgagaagct gttttttctt ggctgttctt
     7141 attatttgat gcaggacagc tttttcacga cctctgtaaa ctataggaat ctgttaaatc
     7201 aagggagcag aacttgcctt ttacgctact tttggtacaa taatgtatga ttaaaaataa
     7261 actggagggt ttctatcttg aacataggta agccgagaaa actaggaaaa acaataaatg
     7321 aaatcgagga aggcgactcg ttatccttaa cagaatctat tgaagataac cagcttttgc
     7381 tgtatttggg attgacgaat gatgcaaatc ctttgtatat ccaacatgac tttgcacaaa
     7441 aaacagaata tcaacagcca atcgttccat cagtgatgct gatggggatc atcacaagtg
     7501 caatttcaaa gcatcttcct ggaccaggat cacatgttgt caatttttct gtcaatttta
     7561 ttgaaccggt tttccattat gaaacactga cttttcagtt tgaggtcata aaagtagata
     7621 agatgaagga tgtggtgacg atttctgtag aagcggagaa tacgaacgaa gatcgtgtgt
     7681 tggatgctgt cgttatggtc caaccgccac aagttcctga attggaaata gcaatcgaaa
     7741 gtgaaggtga attgaatgaa taatccaagt acatcaatgt caggtatcaa tcgtttttat
     7801 gcaaaagttt atggattttt aggtatggga cttgccctta gtgcagtcac ttcttacttg
     7861 gctctttggg tatttccttt gcaattagca acttttatca ataattttcc actaggcttt
     7921 gtgggtctgt ggatcgttga actgatttta gtaatggttc taggcgtaaa agcacaaaag
     7981 aatccgagct tagcaatcgg tggatttatt gcctactctc ttttgaatgg cataacatta
     8041 gcggttactc ttgcatacta cgatattgga acagtaacgc aagctttctt gacagctact
     8101 ttgatgttca tggggatgtc tgttgtcgga gtaacaacaa aaagagatct tagcggggtt
     8161 ggaagagcag gacaggcagc tttaatcgga gtgatcattg cactagtctt gaacatcttc
     8221 attttgagaa gtagcgtagt tgagctgttc atctccctac tgttagtcgt catctttgca
     8281 gggattacag cttatgataa ccaaagcatc cgtaatatct atgtccagac aaacggaaca
     8341 gcacatagtg gaattgctgt attcatggca ttgaagctgt atttggactt tatcaacttg
     8401 ttcttggcgt ttctgagaat ctttggacgg aacaactaaa aaagaaaaaa attcatcagt
     8461 tggggctata ccgaattatt tggtatggtc ccgctttttc ttttcgcatt ccgcaggatt
     8521 ctttgttaga atgaataaga actttacagt aaggagttaa gaagatgaca aaaaataaat
     8581 tattactcgt tgatgggaac agtgtggctt ttcgcgcatt ctttgcgctt cacaattctc
     8641 ttgaacgttt caaaaataga aacggtcttc atacaaatgc aatctatgca ttcaatacga
     8701 tgtttgaaaa tgttatgcaa aaagagcaac cgactcatgt tctggttgcg ttcgatgcag
     8761 gaaaaacgac ttttcggaca gaaatgtatg cggagtacaa aggtggaaga tccaaaacac
     8821 ctggcgaatt caaagaacaa atgccatata tacgtgattt gttgacagga ctaggggtac
     8881 agtattacga attgcccaat tatgaagcag atgatattat cggtactttg gcagaaaaag
     8941 tagacaaaga tcaatttgat gtcgtcgtgc tttcaggtga tcgggatttg actcaattag
     9001 ctagtaaaga agtaaaagtg gatatcactg taaaaggtgt cagcgatatc gaaagctata
     9061 caccagaaca tgtagcggaa aaatatgatg ggttgacgcc aaaacagatt attgacatga
     9121 agggactagc cggagatgcc tctgacaata tccccggtgt gactaaaatc ggtgaaaaaa
     9181 cagctatcaa gctattgaaa caatatggct cagtcgaagg aatttatgaa catatcgatg
     9241 aaatgaagca aagcaaaatg aaagaaaatc tgataaatga caaagagcag gctttcttat
     9301 cgaaaaaatt ggcgaccatc aatacttcag cgcctgtgga tgtctctatc gactcattga
     9361 agtatgaagg gaaaaatctc gataaactcg ttccgtttta taaagaaatg gatttcaatc
     9421 agttcctttc taaattgaat atcgtagaag aagaagtaaa aatggatgat attttgtttg
     9481 aagtcgttca tgaattcaaa gaggaaatgt ttactacgga tatggcgttg tacgtggaaa
     9541 tgatgggcga caattatcat acagaggaaa tcgtcggagt agcgtggggg acagaaaaga
     9601 aaatttatgt cacgaacgat ctatctgtat tcgaaaaccg agcgttccat tcttggatca
     9661 ctgatccgac tcggctgaaa aaagtatatg atgcgaaacg aacttatgtt gctttaaacc
     9721 gctatactgg aaaaacgaaa ggaatcgatt ttgatgtctt gctaggtgca taccttctcg
     9781 atacgaatga aaaaagcaca gatatcgaag gaatagctgc tcattacgga tacaacgata
     9841 tccaatcaga tgaagctgta tacggcaaag gggcgaaaaa aggtttacca gaagaagagg
     9901 aactcttttt tgctcattta gcgcgaaaag tagctgcaat caatgcgttg acaccaaaat
     9961 tgagccaaga actggcagat aaaaaccaag aagatctttt ccggaaaatg gaactgccat
    10021 tgtctatcat tttggcagag atggaaataa gcgggatcaa agtagatgcg actcgcttac
    10081 aagaaatgaa gggcgagttc tctgctcgct taagagagat cgaacaaaaa atctacgaag
    10141 aagccggcga agaattcaat ttgaattcac caaagcaatt aggagtgatc ttatttgaaa
    10201 aaatgggatt acccgttatc aaaaaaacaa aaactggata ttccacagcc gtagatgtac
    10261 tggaacagct tcgtgaacaa gcaccaatcg tagaggatat cctcacctat cgtcaaattg
    10321 caaaaattca atctacctat gtcgagggac tgctgaaagt gatccaatca gatggaaaag
    10381 tgcatacccg ttatgtccag acattgaccc aaacaggacg attaagctct gttgatccta
    10441 atttgcagaa catcccgatt cgattagatg aagggagaaa gatacgtcag gcatttgttc
    10501 ctagagaaaa agactggctg atttattctt ctgactattc acaaatcgag ttacgcgtac
    10561 ttgcgcatat ctcaaatgat gaacatttga aagctgcctt cctagaaggt caagatatcc
    10621 atgccagcac agcgatgcgt gtatttggaa tcgaaaaggc agaggatgtc acaccaaata
    10681 tgcgtaggca ggcaaaagca gtgaattttg gtatcgtgta tggcatctct gattacggac
    10741 tttcacaaaa tctaggaatc agccgaaaag cagcacagca atacatcgat acgtactttg
    10801 aaaaataccc aggtgtcaaa gaatatatgg aaagaatcgt acgcgaagca aaagaccaag
    10861 gctacgtcga aacgctgtat caccgccgtc gatatctgcc agacatcaat tcaagaaatt
    10921 acaatatccg ttcttttgcg gaacgaacag ctatcaatac accaatccaa ggaagtgctg
    10981 ctgatatttt gaaaatcgcg atgatcgaat tggataaacg attaaaagaa acaggtcttc
    11041 aagctacgat gctgttacaa gttcatgatg aacttgtctt cgaggtaccg gaaaaagaac
    11101 ttgaatcatt agataaattg gtgaaggaag ttatggaaca agctgtttct cttcatgtac
    11161 cactgatcac tgacagcagt tggggaaaaa cgtggtacga agcaaaataa acattgttta
    11221 agacagagca atataatgaa ggtgaaaaga aaaaatgcca gaattaccag aagtagaaac
    11281 cgttcgtaaa ggattggaac ggttagtagt aggaaaaacg attcaaaaag ttcaggtctt
    11341 gtggcccaag atcatcgagc aaccagagac acccatcttt gaggcttctc ttgttggcga
    11401 aaccatccaa tccattggac gtagagggaa gtttttaatt tttcatctcg atcattgtga
    11461 actgatctca cacttgcgaa tggaaggaaa atatcaattt acgaaagaaa atacaccaat
    11521 cgataaacat acgcatgtac ttttcttttt tgaggatgga agccaacttc gctacaacga
    11581 tgtacgaaaa tttggtcgta tgacaatcgt cgaaaaagga gcaagtgcaa cttaccgagg
    11641 aatcatgaaa ttgggacctg agcctttgcc agatagcttc ttattagcag atttcgcaaa
    11701 tggactgaaa aagtcccaca aagcaatcaa gccattgctg ctagaccaac gtctagtgac
    11761 tggtctaggt aatatttatg tagatgaagc attatgggaa gcgaagatcc atccagaaca
    11821 accggcgaac acactgcgcc ccaaagaggt agaacaatta cgtctatcga tcattgatgt
    11881 tttggatcgt gcaatagaag ccggaggtac cacgattcgc agttatttga atgcattagg
    11941 agaatcaggc ggatttcaag tagcactgca tgtctatcag caaactggca aaccatgtat
    12001 acgttgtgga acgccgatcg tgaaaacaaa agtggcacaa agggggacgc actattgtcc
    12061 gaattgtcaa aggctaaaaa tcgcaaagta ggatttgttt taggtttgac gggtggaata
    12121 gctaccggta aaagtacggc agccaaagtc tttcagtctc atggatttcc tttgatcgat
    12181 ggcgatgtga ttgccagaga aactgttgag cctggaacgc ttgctctgag gaaaattgta
    12241 tcagtgtttg gccaagaggt attgcagcct gatgggcaac tagatcgagg gaaacttggg
    12301 atgattgttt ttccatcaaa ggaaaaacgg caaaaacttg atcagctatt agatccgttt
    12361 atccgaaaag cgataaaaga tcaaatcgcc tctctaagtt ccaatcatcc tttagtaatt
    12421 gtagatatcc cattgctcta cgaaggtcat tatgaccact atatggatgc agtagctgtc
    12481 gtttatacta cgccagaaac tcagcttcag cgtttgatga aacgtaatca actgacgcta
    12541 gaacaagcgc agcagagaat tagtagtcaa cttccaatcg aagaaaaaaa gcagcgtgcg
    12601 gatattttgt tcgacaacaa tggaacaaaa gaaaaacttg tgggacaaat cgaaaattgg
    12661 ctttttgcta atcgctttct ttgagtatct gtgtcaattt tgctatcctg agtatataga
    12721 aagtgaggga tagcgatggg atcattaaaa aatcaaaagg ataaagtcgt aggtaacgca
    12781 aaagaaaaaa tcggcgagat gctcaataaa gaaaatctaa tcgatgaagg tcgtacgcaa
    12841 tcagaaacag cagaagaaaa agaaaagcag taccaagcaa gaatcgatga aatagaagac
    12901 cagcaggcag aagaagaagc gaagcggcaa gatttgactc aagaacgaaa aaatccgaca
    12961 cctgatcgta gtaaaacacc gctcccagaa agtgaccaca tttccgatga gcaaaaaatc
    13021 gaggaacagc gaatgaaaca ctatacagga atcgaagaga agttataaaa tagtaaaaaa
    13081 tcgagatgtg acttgaagca gatgagaagg tgttcatctg cttcaagttt tttgtgctag
    13141 atgactaaat cagcttttgt gtaagttatt tctcagtaat caaggtgatc agtattggaa
    13201 aattgaaatt tagtaaggac aggggacaca attacgcatg aaagtagaaa aaattgttct
    13261 tatatccgtg ttttttgggt aggacagtcg cgtttgtgat ttgtttcgtg ttataataca
    13321 actagttgta aagaaagaca catcctttat ataaaatgcg atgcaagagg tgagacaaaa
    13381 tgagatgtcc cagatgtcaa cataataatt cacgagtaat cgacagccgt caagcagatg
    13441 atggacgtgc gatccgtcgc cgtcgcgaat gtgagaattg ttcgttccga ttcactacat
    13501 ttgaacgaat tgaagctgct ccgttattag taatcaagaa aaacggggaa cgcgaagagt
    13561 tcaatcgcga taaaatttta agaggactca tccgttcagc agaaaaaaga cctgtagcta
    13621 tggaacaaat ggaacaaatc gtcaaccatg tcgaaaaccg cgtacgcagt ctcggagaaa
    13681 acgaagtatc gacgaatcta attggtgaat atgtgatgga agatttagtt gatttagacg
    13741 aaatcgctta tattcgtttt gcaagtgttt atcgccagtt caaagatatg tctgttttct
    13801 taaaagagtt gcaagatatc gtggataaag caaaagaaaa taataaagag aataataaag
    13861 aaaacaatta agaaacaaaa aggaggcatt tccttggaaa atgcttggaa agagcttcaa
    13921 ccaaaaaata tttaccaagg aagaaaagcg gctccgctga gagaagacag caagaacgcc
    13981 ttgctgtatc tttatcagcc actaatcggc ggagaagcct tgagtatcta tttcaccctg
    14041 ttatcagaaa tctctcaaga atctggcaaa ggtcctgaag ggatgcatgc agatctgctt
    14101 agtagtctag gctgcggtat cccgcaattt tatgatgcaa gaaaaaagct agaaggaatc
    14161 ggcttactgg atgtttattt taaagaagat agtgatctgg gacctcgttt tatttatgag
    14221 ctgttagaac cactcattcc taaagcattt ttcaaagact ctgttctggc ttttcttctt
    14281 ctggaaaaag ttggtgaacg acgattccag aagcttaccc aattctttga acctaaaagg
    14341 catacagcag agggatatca aaaaatcaca aaaaaatttt tagatgttta tcgcttcaat
    14401 gaagcagtgt acgctgctaa tcaagaaaac atcgaatcgc tgaataaatc ttttgaagaa
    14461 tctgaatcag tagcattact tgaagcagat tcgacattcg attggcagtt tttgaatgat
    14521 tggctggcaa aaaaacatat tgcaagtctt gatgatccaa tcatcaaaca agtcgacatg
    14581 tatcatcagc tttacggttt cgatgaattg acacttggcg aactgatcag tcaatcttat
    14641 gactttagtg aacaaaaagt atctgaaaaa gaactgcaaa aagccatttt ggcaatgcag
    14701 agacgtccgg tgaagcaaac aaaagaaaca gttgcggttt ctgaaaacag taatgaaccc
    14761 tcgtcaaacg aacatttgac aaacgcagcg cttcagctga taaaagaagc acagagcatt
    14821 ccaccgatga aatatctgga agcaatcaag tcagaaaaag gcggatatgt ttctaaacaa
    14881 gaaaactggt tactgcaaga tcttgtttct caatcaggtc tttccagtag cgtgatcaat
    14941 gttttgctga attatgtact agtgatcaag aataatgcta gcttaaatgc cagttatgta
    15001 aatacagtgg caaatgaatg ggcacaaaag aaaatcgcca cagcagaaga agcgatccag
    15061 cacattcggc aaataagtaa tcaagcaaaa caatcaaaac aaaagaagca aacaaattat
    15121 caaactggca agcgaaatgt tcgacgggaa aagcttcctg attgggtcaa ccagccaaaa
    15181 gatgaaaaac agatcagcag cgagaaaaaa gcagagattg accgccgttt taaagaatac
    15241 ctggcaaaaa aggagggtga tatctagtgg aagacgtagg caaagaattg aagaggatca
    15301 tcagcaaacg caattatcaa gaaaagtatg ctgaaatgat cgctgaagtc ttaaatgatg
    15361 cggatgttca agcttttcta agcgcacacc aagaagaatt gacacaagcg gatattgaaa
    15421 aaagttacgc taaattgtat gaattcgtgc aagaaaagcg taaatatcaa aaaaacgatc
    15481 caacgatgat cgctcccggc tatgagccga agctgacgtt gaattttcat tatgtggatg
    15541 tcacttatgt accaacggaa gcattgttag cacggcaaag acaagaagaa atcagaagcc
    15601 gagtaaaagc aatggatatg ccaaaagata ttcaatcggc ttcttttgaa aactttgaac
    15661 gtacagatgg acgaggatat gcgagcttag aagccctaga ttttgtagag aaatatactg
    15721 ctgatccaaa agggtatcat aaaggactgt atctagtagg aagttttggt attggaaaaa
    15781 catatttatt aggagcaaca gcgagagaat tagcgattgc tggattcacc acgacgctag
    15841 tccattttcc gacatttgcc gttgagatga aacaggcaat cggaaaagat caagtggcag
    15901 aaaagttaga tgcagtgaaa cgttcaccta ttttgatgat cgatgacatt ggtgcagatg
    15961 cgatgagtag ttggatacga gacgaagtat tcggtgttat ccttcaatat cggatgcagg
    16021 aacagttacc aaccttcttc tcatcgaatt ttacaatgga tgaattggaa cagcatttgt
    16081 ccgtcactca acgtggagat gaagagccgc taaaagcgaa acggataatg gaacgcatac
    16141 gttatttgac aaaagaaatc gaaatgactg gtcgcaaccg acgcaatggt tgaacagttg
    16201 acaagtccaa ctgacatttg acctagtagt aaaagcaggt cagcgaatca ttatatggac
    16261 agaaatgacc ggagacaatc accgcaacaa gtaatgcaga aacttaaacg gaggattttt
    16321 tatgagggct attttttcta gtatatctgt agttggtcta ttttttcttt tagcaggctg
    16381 cggtagcgca gatagcaaca caggttctgt tgattcgatt gatgcgggtc aaacagtaaa
    16441 aacaagcagt acgaaaacgg agagacaaag catatttgtc ggaacgattg aaagtattac
    16501 tcaagtagaa gcagaagaag aaacgcttca gttgaaactc gtcgatgtca tggccaaaga
    16561 agatccagcg aatatgggaa cgtcattcag caatgacgga gtgatcttaa atgctactgc
    16621 tgaaaaaata agcgtggaca taaatgaatt gaaaatcggt gacaaagtcc aatttacatt
    16681 agttgagatg ccgatcatga cgatgtctat cccaccgcaa gttcctggag attcgattat
    16741 tgatgtttca ttgaacaaat aaccaataac attttttcaa aaataagaaa atgttgtttt
    16801 atagaacgaa aaggtatatt ttttaaggag atatgggaat ttttttattc ctagtctcct
    16861 tattttattt ggaaaatgct ataattatct agaaggagtg aagctatgaa ccatttagat
    16921 ttatctcagc gcttagccgc agtgggtgac tttgtccctg aatccgctcg tttagcagac
    16981 attggatcag atcacgctta cttacccgtt gcattgatat taagaggaaa aatcgaatat
    17041 gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag cacgtaagca agtcaaaaaa
    17101 gatggattag aagaccgtat caccgtacgc cttgcaaatg gtctagatgc tatccagcca
    17161 caagatggta ttacagctgt cacgattgcg ggtatgggag gttcactgat ccgagatatc
    17221 ctagaagctg gcaagttgaa tcaacgctta agcggacaag aacgtttgat ccttcagcca
    17281 aacatcggag agcgtacatt acgatcttgg ctgcaagaaa atcagtataa aatccaatca
    17341 gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca ttgtagccga aaaagaacaa
    17401 aaaccagtta cttacaatga aatggagctg atgttcgggc catttctgct tgaagagaaa
    17461 agtgagatct tcaaatcaaa atggacaaga gagcttcaac aaagaaaagc tatccgtgaa
    17521 cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac aaatcgagca ggaaatccaa
    17581 atgattgagg aggttttaaa gccatgacaa tcagcggacg cactttcatc caaaagtttg
    17641 aaagctattg tcctcagtgg ctagctgaag aaggagaccc agtcggactg catatcggaa
    17701 cattggataa gccaatccaa cgagtaatga tgacattaga cgttcgtcca gaagtggtcg
    17761 aagaagcaat caagaaaaaa atcgatttgc tgattgctaa acatcctcca atctttcgtc
    17821 ctgtaaaacg gctggttact gatcagccac aagaaaaaat gtatgcagat ttgttgaaac
    17881 atgatattgc cgtctatgca gcacacacga acatggatat tatttgggat ggactgaatg
    17941 actggttttg tgaacttctc ggtattgaag tggagagtta tttagtaaag actcatgaga
    18001 ttcattataa aaaactggcc gtttatgtac ctgtggatca tgcacagaaa atgcgtgaag
    18061 tattagctgc agctggtgct ggtacacaag gtgattatac cggtacaagt tttacctcga
    18121 ttggacacgg tcgatttact ccggaagctg gtgcacaacc agcgattggg aaagtaggga
    18181 agacagaaca agttcaagag gcaaaagttg aggttatttt gccggaaact atcgaaaaac
    18241 aagtgattca agccatgcgg tcagctcatc cttatgagga acctgcttat gatcttttcg
    18301 ctatagatga accagtcgaa atgtttgggt taggacgagt aggtgaattg ccgcaagaaa
    18361 tttctataga ggcatttgta gaacaagtaa aagaagcgtt ccaactagat ggactgagaa
    18421 tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat tgcgatttgc ggtggaagcg
    18481 gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga tgtctatatc acgggagata
    18541 tctattatca tacggctcat gatatgcaaa gcgctgggtt gattgcaatt gatcccggtc
    18601 attatattga atcgttatgc aagcaacgat ttatcgagaa atttgaatct tggaaacaag
    18661 aagaaaattg ggatattaac tttttcgttt ctgaaacaaa tacaaaccca tttcaattta
    18721 attaaaagga gagaatacta tgtacgaaaa tcttttgcca cgttttttac gatatgtcaa
    18781 aacagaaaca cgctccgatg caacaagcaa aacgactcca tcgactcaga cacaagttgc
    18841 ttttgcgcaa gaattgaaaa aagaattaga agagttaggc ttagctgatg ttttctataa
    18901 tgaagataac ggattcgtta tgggaacatt accaagcaat gttgatcatg aagtgcgttc
    18961 cattggattt atttcccaca tggatacagc agattttaat gcagtaggag tcaatccaca
    19021 aatcattgaa aactatgatg gcgagtctac gatcgtacta gatgcggaag aacgctatac
    19081 attgaataca aaagattttc caaatttgaa aaattatgaa ggccacacat tgatcacaac
    19141 agatggcagt acattactgg gagcagatga caaatcaggg attgcggaaa tcatgactgc
    19201 aatggagatc ttgatcaata atccttctat ccctcacggt accatcaaag tggcttttgg
    19261 tcctgatgaa gaaatcggtg tcggagcgga taaattcgat gttgagcaat tcgatgtaga
    19321 ttttgcttat acagtcgatg gtggtccttt aggtgagttg cagtatgaaa cgtttagtgc
    19381 tgctcaagca aatattacga tccaagggaa aaatgtacat ccagggacgg caaaagatac
    19441 aatgatcaat gctctacagt tagcaatcga ttttcataat cagcttccag ccgatgaagt
    19501 tccagaaaaa acggaaggat acgaaggttt cttccatttg atggcattgg atgggaatcc
    19561 tgaagaagct tctatgagtt atatcattcg cgatcatgat cgtgaaaaat tcgaagcacg
    19621 caaagcacaa atcacacata tccaagaaga gttgaatcag cgatttgatc aagaacgaat
    19681 caaagtagat atgtatgatc aatattacaa catgaaagaa atcattgaaa aagatatgag
    19741 catcgtggaa ttagcgaaac aagcaatgat cgagcttggt atctctccag tgatcgaacc
    19801 agttcgtgga ggaacagatg gttcaaaaat ctcttacatg ggtattccga cgccaaatct
    19861 atttgccgga ggagaaaata tgcacggacg attcgaattt gtttctttac aagtaatgga
    19921 aaaagcaaca gacgtgattg taaaaattgc agaattgaac acaaagagtt aaaatcgaaa
    19981 aagaacagct tatgacaagg cgttcgtccc aatgatggaa tgtcatctgt ctaaggctaa
    20041 agggcaggac aatggaattg gttgtcccgc cctctttttt gaagagtaaa taggaggtaa
    20101 agaatggaaa agctgctagt tttttataac aaaagttcag ggaaagatga gggtgagaaa
    20161 ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc cagaattata tgtagaacta
    20221 gctgaaactg gcccatcgat tgatgatgcg gaattagtca agaaagcaga agccatagaa
    20281 gctgatactt tagtaattat aggtggagac ggcacgattc atcatatcgt ccaagcgttc
    20341 caaaaaacat tatctcaata ccatgtcgga ttacttccag gtggaactgt caacaacctt
    20401 gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg ctgacgtgat cctggaaggc
    20461 aatgtacgga aaatagacta tggtcaagtc aatgatgaag tcattatctc tacactgaca
    20521 atcggaatct tagcagatac agcagtttgg atcagtcaag aagaaaaaca gcgttacggc
    20581 tcgtggatct ttatcaagaa gttttttaag ttattgctga agaagaaaaa atatcatttg
    20641 gatttagaaa cagacgagga gcattggaaa ggaaaatccc aactgttgac agtcacgatg
    20701 tctaactccg tgggcggttt tacaaatttt gacgactcag cgaccctaga tgatggaaaa
    20761 tttcatgtaa cgatcgtacc ttccttagat attttccgat ttatttttta tttgcctaga
    20821 atcatgagag gcaaattcta tacgatcccc ggcattactt atttcacagc caaacgcatg
    20881 aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg atggagacac gacagatgat
    20941 cttccagttg aattatctgt tgtatcaaaa ggattaacag tttattcacc aaagaaagaa
    21001 gaaaaagctt agaaatagta gttaacagaa agttggaaga agagtaatgg atgtattgtt
    21061 acacaatctt ttgtggtttt accttttcat tgtgaatttt tacttatttt gtctgatggg
    21121 gtatgataaa catcaggcta gaactagcgg ctggcgtgtt cctgagaata atttgatatt
    21181 tgtcagcctg ttaggcggtg gtttgggagg attgatctct cgaaaagttt tccatcataa
    21241 aactcggaaa aagaagttta ctataggatt cgctgcaggt gtacttgttg atttgattct
    21301 catttatttt ttccattaag gagcaggaga tgtcgtctaa aggaacgaaa actaaaatca
    21361 ttctgaatct gcttttgctg attgtgattt ttgccattat ttattattta atcaatcaat
    21421 catttgcaga tatctttgcc gaattgctgt ctaccagtgt tgttgttttg gcagcggctg
    21481 ttatattagg gacagtttac caaatcgctg aagggcgctc aatcaaagaa attgctgaac
    21541 ctttcgcgcc gaattttaca acgaaagatg ggttcttgac ctctagctat attgcgtttt
    21601 accgaatcgt ttcgtttggt acaggaacgc tgatctcgga gatttatttt tataacaaaa
    21661 aaggaatgaa gctatcccag ggagcagggg tgacagcact ccatatgatt atgtacaaac
    21721 tggcagtgat cacttatgca ttagctggct tggtcatcca attctctctt ttttattcta
    21781 atggtccatc aatgatctgg tttatcatcg ctggtatcct tttgacgatc gtgattgttg
    21841 cctttttatt ggcgatgtcc gtcagcttga atgttcaaat attttttgtc agcttatcga
    21901 ataagttatt taaaagtgag cgtttgcgga aatgggtgga tacatgcaat cagcagatct
    21961 actctttaag ggaaacggtc caaaccatta tccaagacag gactgcgcta ttgcggattt
    22021 atctatggaa tatgttaaaa cttgtatttt ggtatgtact tccttatttg attctgattg
    22081 aaaaccatcc taaaatagat tttctactga ccatttcgtt tatcagcttt gcagtagtgc
    22141 tttcaggggt tattcctacg cctgctggta ttggttcctt tgaatttgtc tatttgttat
    22201 tattcagacc agttgttgga acagttgacg cagtttcttc gcttttactg tatcgtttca
    22261 gttcctttat cttgccattt ctctatggat ttgtgtatgt gattgctgaa cggcgaagag
    22321 tgatcaaaga agaaataata gaagtaaaaa aagagaataa agaaaatcaa gggtaggtct
    22381 tttataagat ctaccctttt tatcaaatca aagaaaaacg ttatggataa gataatttta
    22441 cgtttgtttg gctctttttt tatatacata caatcaattt tgttttttta agatttatat
    22501 aagtcgagaa aaaaagtcaa aaaaccatgt gtatattttg accaaaattg accaaagcga
    22561 tataatagtt tttgtaaaga tcaaagaaat aagtgatctt acctttaatt aggagggtat
    22621 acctgtatga atattgagaa aatgactacg acgcttcaag aagcgattgc tgaagctcaa
    22681 cagattgcgg tcactcgaaa acatcaagat atcgacattg cccatgtatg gaaaatcttt
    22741 ttacaaccta atcattttgg tcgaaatttc tatacggatg ccggattgga tgtcgaatct
    22801 tttgaacatg aaattgatcg tctactggat gagtatccag ttgtttctgg tggaaacgtc
    22861 caatatggac aaaacctcag ccagaatttc tttcgattat tgaatgaagc agaccaaata
    22921 cgtgaaagct tcggcgacga gtttctttca acggaagtcg ttattttagc gttgatgaaa
    22981 ttaaaaaatt atccgttaac tgtttatttg aataaaaacg gattatctga aaaagaattg
    23041 cgtaaaaaca tcgaagagat gagaggagga gaaagagtga cctcacaaaa ccaagaagaa
    23101 caatacaaag cattagaaaa atacggcgtt gatttagtac agcaagtcaa aagcggaaaa
    23161 atggatccaa tcatcggtcg tgatgaagaa atccgtgatg tcatccgtat tctttcacga
    23221 aaaacaaaaa ataatcctgt tctgatagga gaacccggtg tagggaaaac ggcaatcgtt
    23281 gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg aaaatttaaa agataaaacg
    23341 attttttcat tagatatggg tgcattgatc gctggagcaa aattccgtgg tgaatttgaa
    23401 gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg atggacgtat cctcttgttc
    23461 atcgatgaaa tccacaatat cgtaggggca ggaaaaacag aaggaagtat ggatgccgga
    23521 aatctgttga aaccaatgct tgctcgcggt gaattacatt tgatcggtgc cacaacttta
    23581 gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg aacgtcgctt tcaaaaggta
    23641 ttagtcaaag aaccaacagt ggaagatacg atcagtattc tgcgtggatt aaaagaacgt
    23701 tttgagatcc accatggtgt taacatccat gacaacgcat tagtagcagc agctacttta
    23761 tctgaccgtt atattactga tcggttctta ccggacaaag caatcgattt gatcgatgaa
    23821 gccagtgcaa cgattcgggt agaaatgaac tctatgccaa cggaattaga tcaagttact
    23881 agacgtttga tgcagttgga aatcgaagaa gctgcactga aaaaagaatc agatgatgca
    23941 agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg aacttcgtga agaagcaaat
    24001 gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca attctgtttc agcaaaacgt
    24061 gctgaaatcg ataaagcaaa acacgaatta gaagatgcag aaaataacta tgatttagaa
    24121 cgcgctgctg tattgcgtca tggtacgata cctcaattag aaaaagaatt gaaggaatta
    24181 gaagctaaag caaaagacag cgagattaag atggttcaag agtccgttac cgaaaatgag
    24241 attgctcaag tcgttggcag attaacgggt atccccgtaa cgaaacttgt agaaggtgaa
    24301 cgagaaaaac taattaaatt gaatgaaaca cttcataaac gagtgattgg tcaagatgaa
    24361 gcagttgatg cggtcagtga cgcggtaatt cgttcacggg ctggattgca agacccaaat
    24421 cgtcctctag gttctttcct tttcctagga ccaactggtg tcggaaaaac cgaattagcg
    24481 aaagcgttag ctgagaactt gtttgactca gaagatcata tggtacggat cgatatgagt
    24541 gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag ctcctccagg ctatgtaggt
    24601 tacgaagaag gtggtcaatt aacggaagct gtcagacgaa atccttatac aatcgtcttg
    24661 ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata tcttgttaca agtcctagat
    24721 gacggacgct tgactgattc aaaagggcga gtagtagatt tcaaaaatac ggttctgatc
    24781 atgaccagca atatcggttc acagttactg ctggaaggcg tgactgctga tggaacgatt
    24841 ccagaagcag tggctgaaca agtcaacacg ctattacgtg gaaacttcaa accagaattc
    24901 ttgaaccgaa tcgacgacac gattttattt acaccattga gcctagacaa cgtgaaagga
    24961 atcgttgaca aaatggtggc gcaattggca caacgtctgg aacatcaaga aatcctgctg
    25021 acaatcagtg atgaagccaa aacgtggatt gctgaaaatg cttatgaacc agcatatggt
    25081 gcaagaccat tgaaacgatt tatcactaag gaagtagaaa caccactagc taaagaaatc
    25141 gttgcaggac atgttatgcc taaatcaaaa gtaacgatta ctttacttga tggacaactt
    25201 catttcaaaa cagaagagct agaagaaatc gtctaatctt ccattgagac tataaaagaa
    25261 aaaaggggtc tgacacaact agtgtcagac ctcctttttt caaataaaca gtgttccaga
    25321 agtagtttct tcgaaaaata agccgaaatt tcttgaagct gcccgcttct gtcacaacct
    25381 cttttattag gtatttttca ctttttctaa tttataactt tcaaagtaac gctcatcttt
    25441 tttactttga gaccatgcaa acaatcccat taaggcagca cctatcgtat ttgtcagcaa
    25501 gtcgcccatc gtatccatca atgctgctcg tccgacaaac agtgtgccat cagaagcagc
    25561 aaaacgttga agattcatgc ctaagaactg gtcgcataag aattcccaga actcccaaaa
    25621 cacgccgcaa agtccagcaa acgcaaagcc gaataagagg aacaaccaag gactggtttt
    25681 tgatatctca gcatctttca tcaagtaacc aatcaatcca tagccaagag ctgtcaggac
    25741 cattggactc accgcatgta agattttgtc ccaaaaagag atgatactga tcaaatgcat
    25801 ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag taaaacaaaa cgattgctgc
    25861 tggtatttta agtttgaaaa ctttcaaaat gacttgtggc atgaaaatca atgcaatccc
    25921 taataaagat tccacgataa atgtgattcc ttgagaagtc gaatattttt gtgtgataaa
    25981 ttcataaaaa ttatagcaga caacgaatac accaaaagca agtaaagcta tttgcacctt
    26041 tttgatttct ttaatcactg aaagtccctc catctaaaaa tctttttact tctgaaagca
    26101 actcaatttc tgaagaaacc acttttccgt tcaatttgat caaacctatt gtatataagt
    26161 tcaggtagtg aaattgattt tcagctgttt cttgtagtgc agcgattttt ttcgggtggg
    26221 ttccgccttg ctgtcgagta tcagtatata agccgatcac tgggatgcct ttcgcataag
    26281 ccacaccgat ttccgatgct accccggcat cgatcgtcag cccatctagt aaagcaatca
    26341 ttaaatcact agccaacact ttttctgtgt cagcaagagc aatcattcga ctgtctgcat
    26401 aagcagtttt gtcattgatt gcttcatttt cttgaggaag atagatcgaa agctcagaag
    26461 agagctctct gatttcttta actaactgtt ggttatataa caaatcactt ttagcaaaca
    26521 ttggtgcggc aaaataaatt ttcatctaaa catcatcctt tttatcaaaa ataatcgcta
    26581 aagtaagctt ccatgtttgg aatgatacgc tctagtgact tcagtgtttc ttcatctgtt
    26641 tctaaccgtt cgattcttgc caaataagaa gcacggcgat aattcatcat caagcaagtc
    26701 aatgtttgga tatccagtcg aacggatttt cctataggtt catttaagac acggacttgt
    26761 ccctgttcat cccatgttaa agcgaagaca ccattattcc aaggagcgac aggatcttca
    26821 atgatgaaat gaaaagcatc agctgttcca acaaacggaa accgttgaag gaattctttt
    26881 acatcaacga tccgagccat aaaatatggt tcgatctgct ctttgatctg actatcttca
    26941 atcaaaaatg ctaaaggctc atttttataa atatctccat gtacccagta gatcatggaa
    27001 aaatgtgcgg aaataaagtt ccataagcca tttcgagctt cttgatcaag atagaacatt
    27061 tcttttacgt gaaatatttc ttcagctacc cagtagaaca gaacacctct tggttcatgg
    27121 ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat tttcgaaacg ccaatactct
    27181 tcccaatgaa aatcactgcg gaataaagca ccatggtttt gttgggcaaa tttgtcataa
    27241 acggaaaata cgtctggatg atcaactggt aatcgctcaa ccataccact tactgggatt
    27301 tgtttaggta gctgtgtatc tctgatttta aaggaaagtt tatcagacat gatttcccat
    27361 cctttacgcc gatagtaggg gatactgtaa ggatacaaat aagagatcca ttgcttattc
    27421 ttgcgcatat tatccaatgc aacgataatt agatcttgca tcaatccatg gttagcatat
    27481 tctggataag ttccaactcc agtcacacca cccattttat aacgtgcacc gtgaatattg
    27541 acttcacaag gatagatagc gatttgagag atcagtttat tttcgtggaa ccatccgaac
    27601 actttcgata cttccaaaat cggttttttg gaacggataa aagctcgttt attctcaaac
    27661 ccgctttcct cgatgtctgc ttcagtcact tggaacacat aggaaagcaa ttcattgaat
    27721 tgatcgatat gttcttgagt gaccggtttt aattcaagat tttctctaaa atctttttga
    27781 tccatttttt catctccatt tattagcttg ataacagtat agcaaaattc tacaaaaaaa
    27841 acttctatag ctgactaatt aaaaactctt taaattactg cttttcgttg gtataatgga
    27901 aagtgctgat tatatgaaaa gaggagacgc tatggatata aagaaaatga aagaacgcca
    27961 agagcagatt cgtaattttt cgatcatcgc tcatatcgac catgggaaat ctacattggc
    28021 cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc gaaatgcaag atcagctgtt
    28081 agactcgatg gatttggaac gtgaacgtgg aatcacgatt aaattaaatg ctgtagaact
    28141 caattacaca gcgaaaaatg gagaaacata tatctttcac ttgattgaca ccccagggca
    28201 cgtcgacttc acctatgaag tctctcgaag ccttgctgcc tgtgagggtg ccgttttagt
    28261 ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc aatgtttatt tagcgttaga
    28321 caatgatttg gagatcctgc cagtcatcaa taaaatcgac ctacccgcag cagatcccga
    28381 acgtgtccgt caagaaatcg aagacgtaat cggaatcgat gctagtgaag ctgtattagc
    28441 cagtgccaaa tcagggattg ggattgaaga tattctcgag cagatcgtgg aatatgttcc
    28501 tgcaccaagt ggagacttag atgcaccgct aaaagctttg atctttgact ctatttatga
    28561 tagttaccgc ggtgttgttc taaatgtccg gatcacggac ggtatggtca aacccggaga
    28621 caaaatcaaa ctgatgagta atggcaaaac atttgatgtt acagaggtcg gggtcttctc
    28681 gccaaaagca gttgctagag actttttgat ggtcggtgat gtcggttata tcacagcaag
    28741 tatcaaaact gtccaagata cacgagtagg ggatacagta acgttagcag acaatcctgc
    28801 cgctgaagct ttaccaggtt accgaaaaat gaatccgatg gtttattgtg gtctttatcc
    28861 aattgatact tctcgttaca acgatttgcg ggaagcttta gaaaaactgc aattgaatga
    28921 tgctgcttta caattcgaac cagaaacctc tcaagcatta ggttttgggt tccgctgtgg
    28981 attcctcgga cttcttcata tggatgtcgt ccaagaacgc ttggaacggg aattcaatct
    29041 agaattgatc acgactgcac catccgttat ttaccatgtt aataaaacgg atggtacgac
    29101 tgctacagta gataacccgg cagattttcc tgaacctgta acgattcagg atgtagaaga
    29161 accattcgtc aaagcacaga tcatggtccc aaatgatttt gtcggtgcag taatggaact
    29221 ttctcaacga aaacggggag aattcattac gatggattat ttagatgatt accgagtaaa
    29281 tgtcgtctat aatattccgc tttctgaaat tgtctttgac tttttcgaca aattgaaatc
    29341 aagcacgaaa ggctatgcct cattggatta tgaaatgtct ggttatcaaa aaagcaagtt
    29401 agtgaagatg gacatcttgc tgaatggaga aaaagtcgat gcattaagct ttatcgttca
    29461 ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa aaactgaaaa aactaatccc
    29521 acgccaacaa tttgaggtgc caatccaagc agctattgga caaaaaatcg ttgcccgttc
    29581 agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt tatggtggag atgtttctcg
    29641 taaacgtaaa ctcttagaga aacaaaaaga agggaagaag cggatgaaac aaatcggctc
    29701 cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa atggacgagg acgaaccgaa
    29761 gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa accatgttta catggatttg
    29821 ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa tcaagaattc tttctaagat
    29881 tttccatgat atcgaccgtt tcatttctca tcttatctgt tacatgcgag taagtatcca
    29941 tcgtaattga aattctgctg tggcccaatc gttcagagat ttctttcatt tttgcgccat
    30001 tttcgagaag aagtgtagca tgagtatgtc tgagagaatg gaagttaaaa gagagggaga
    30061 gtgcattcga tatcctccta gtattccatt tcactacact tggcgtaact agctcaccgt
    30121 cttccttcgt acatactgca tttgaatcaa tgtagagctt tccatacttc attcgatttt
    30181 ctaattgttg tttcttatgt tttttcagaa ttgctagcaa ggtttgtcca ataaaaatcg
    30241 ttcgattgga actgcttgtc tttggcgtac catataccca tgcgccgtca ttctttacca
    30301 tttgtttctc tacagtaatt gttccatttg aaaaatcgac atcatcccac gtcagaccac
    30361 aaacttcgcc aacgcgcatt cccgtataaa atccaatatt caaaggaata tagaaaggat
    30421 gtccttcagg agtaatttct agcatatgat caaagtcctc aagagaaatg atttttagat
    30481 cttttttagt cgttggtcgt gcttcatatt ttggtatttt tacatacagc ataggatttt
    30541 gcttgattaa tccccaagga taaaccgcca tattcagcgc attcttaagg acagagtgag
    30601 taatagtcat tgttttcttc gagtaaccct ttttaaattc agcattgatg aaattttgta
    30661 aaagagcagg ggagagatcc gtaagttttt tctttcctaa ataaccgttt atatgatttt
    30721 tgatggtaaa tcggtagttt tcataggtat tgtattttag atttagttta acgtattcct
    30781 ccatccaaaa atcaaggtat tgttttactc gagtatccgt acctaaaaag tattgtcctg
    30841 tttcgtcaat atctgataaa acttttcgta aagcagcttc ggcctctggt cgggtgtctc
    30901 cgccaacttt ctccactttt tttcttgagc catcatcatt gatatct
//
LOCUS       DACCCS010000031        30427 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.031, whole genome shotgun sequence.
ACCESSION   DACCCS010000031 DACCCS010000000
VERSION     DACCCS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.031"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(1273..1938)
                     /locus_tag="IVK82_001468"
     CDS             complement(1273..1938)
                     /locus_tag="IVK82_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6392539.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="IVK82_001469"
     CDS             2064..4100
                     /locus_tag="IVK82_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP6392540.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="IVK82_001470"
     CDS             4104..4742
                     /locus_tag="IVK82_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6392541.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="IVK82_001471"
     CDS             4770..5198
                     /locus_tag="IVK82_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392542.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="IVK82_001472"
     CDS             5655..6566
                     /locus_tag="IVK82_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6392543.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6783..7220
                     /locus_tag="IVK82_001473"
     CDS             6783..7220
                     /locus_tag="IVK82_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392544.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7315..8199
                     /locus_tag="IVK82_001474"
     CDS             7315..8199
                     /locus_tag="IVK82_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP6392545.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8423..11590
                     /locus_tag="IVK82_001475"
     CDS             8423..11590
                     /locus_tag="IVK82_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392546.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11610..12155
                     /locus_tag="IVK82_001476"
     CDS             11610..12155
                     /locus_tag="IVK82_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP6392547.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12183..12638
                     /locus_tag="IVK82_001477"
     CDS             12183..12638
                     /locus_tag="IVK82_001477"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP6392548.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12635..13393
                     /locus_tag="IVK82_001478"
     CDS             12635..13393
                     /locus_tag="IVK82_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP6392549.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13479..14219)
                     /locus_tag="IVK82_001479"
     CDS             complement(13479..14219)
                     /locus_tag="IVK82_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6392550.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14280..15533
                     /locus_tag="IVK82_001480"
     CDS             14280..15533
                     /locus_tag="IVK82_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6392551.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            15855..16082
                     /locus_tag="IVK82_001481"
     CDS             15855..16082
                     /locus_tag="IVK82_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP6392552.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            16205..16531
                     /locus_tag="IVK82_001482"
     CDS             16205..16531
                     /locus_tag="IVK82_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP6392553.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELVDVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            16599..17312
                     /locus_tag="IVK82_001483"
     CDS             16599..17312
                     /locus_tag="IVK82_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP6392554.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            17622..18473
                     /locus_tag="IVK82_001484"
     CDS             17622..18473
                     /locus_tag="IVK82_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6392555.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            18594..20459
                     /locus_tag="IVK82_001485"
     CDS             18594..20459
                     /locus_tag="IVK82_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6392556.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            20507..21916
                     /locus_tag="IVK82_001486"
     CDS             20507..21916
                     /locus_tag="IVK82_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP6392557.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            22024..22725
                     /locus_tag="IVK82_001487"
     CDS             22024..22725
                     /locus_tag="IVK82_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6392558.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            22884..23363
                     /locus_tag="IVK82_001488"
                     /pseudo
     CDS             22884..23363
                     /locus_tag="IVK82_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            24211..24786
                     /locus_tag="IVK82_001489"
     CDS             24211..24786
                     /locus_tag="IVK82_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP6392559.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            24786..25226
                     /locus_tag="IVK82_001490"
     CDS             24786..25226
                     /locus_tag="IVK82_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP6392560.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            25242..25943
                     /locus_tag="IVK82_001491"
     CDS             25242..25943
                     /locus_tag="IVK82_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP6392561.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(26431..26856)
                     /locus_tag="IVK82_001492"
     CDS             complement(26431..26856)
                     /locus_tag="IVK82_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392562.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            27230..28201
                     /locus_tag="IVK82_001493"
     CDS             27230..28201
                     /locus_tag="IVK82_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6392563.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            28233..29630
                     /locus_tag="IVK82_001494"
     CDS             28233..29630
                     /locus_tag="IVK82_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6392564.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            29789..30412
                     /locus_tag="IVK82_001495"
     CDS             29789..30412
                     /locus_tag="IVK82_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392565.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT        10325 a         4936 c         6269 g         8897 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta
     6661 cttgatttag ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat
     6721 atttttctgt ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat
     6781 aaatggagtg gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg
     6841 tttttctcac aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc
     6901 atcaattttc cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta
     6961 ttcaaggccg tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc
     7021 ctctgatcgc tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc
     7081 tatttttgtg tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat
     7141 cattttcttt tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt
     7201 tttatcttgt caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt
     7261 ttttctttat gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa
     7321 aaagttttat tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa
     7381 ttagcgatag caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa
     7441 gcaggggatg caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt
     7501 gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat
     7561 cggccaaatt tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg
     7621 ataccgctag aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg
     7681 gatattggaa aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct
     7741 gaatcaatca atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc
     7801 atgtcaggaa tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat
     7861 ggaaagaagg aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc
     7921 ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat
     7981 ttaaaagatt cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta
     8041 gatgaatcta cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct
     8101 gatgaggaag aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca
     8161 atcaaagtat tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga
     8221 gttggcggga gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc
     8281 cttttttttg tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca
     8341 tattttaaat atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa
     8401 acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt
     8461 gtttgtcttc cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga
     8521 atcaacgcaa tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac
     8581 ttctgaaact acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa
     8641 gacaagtgag acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc
     8701 aagagctgtt tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct
     8761 tctcgcagac gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga
     8821 attagagatc aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac
     8881 acaccatatt tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac
     8941 agctgcacat ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta
     9001 tatgaccgat ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac
     9061 acttgatttg tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac
     9121 attagatggt gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc
     9181 tccgggtaaa gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact
     9241 ttatggaccg tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag
     9301 tggaaaaaca gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga
     9361 tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat
     9421 gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa
     9481 tgacaacaat cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa
     9541 tggtgggaaa atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc
     9601 tagtcaaatc catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga
     9661 tcaacccggg aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg
     9721 agccggatca gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga
     9781 gaatggtacg caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg
     9841 tgatggcaaa tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga
     9901 cgcttctggt tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc
     9961 aaatatcgat atacgcgcag attccggagc aactgtagat acaactggag atattgattt
    10021 aaattttacg gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa
    10081 gaccggcatc atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga
    10141 tttccaaaac aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg
    10201 aaatggagat tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg
    10261 cgcaacattc gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc
    10321 gatcaatggg accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga
    10381 atttgctcag acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct
    10441 cccgatgatt gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt
    10501 cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt
    10561 gactttacgt attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac
    10621 gcaaggacaa acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca
    10681 aggcggcttg ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt
    10741 gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc
    10801 caaaacgaca gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa
    10861 tgcagcttct cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa
    10921 aaatcaagct gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat
    10981 cccagaaaat caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg
    11041 agaagctact gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca
    11101 caatccaagt gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa
    11161 gatcgtagta gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat
    11221 caaagcaact ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg
    11281 gatttatgat cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc
    11341 tttcacaaat gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga
    11401 gtatcaatta gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt
    11461 gcaagcagat tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa
    11521 agaaaatcag atagctggta attactcagc agagataaca tggacattag cagatgcgcc
    11581 gacagaataa agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa
    11641 acaactgctc tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc
    11701 atgcctccga attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga
    11761 acaaaacata tataaatgta aggatggagc ctgggaaaac acaggaaatc ccctacacgg
    11821 tctttaaccg aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga
    11881 attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt
    11941 atgatatgaa aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg
    12001 tcactgagac actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag
    12061 ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca
    12121 atcagttttc tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag
    12181 atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga
    12241 atcttcaaaa tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca
    12301 aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca
    12361 atacaaattt tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc
    12421 aagcggttgt gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa
    12481 taactgatga tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag
    12541 aaacatttga ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg
    12601 gattatttat tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac
    12661 cgttctagct ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc
    12721 agccactaca gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt
    12781 tgttccagtt cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc
    12841 aaatcaggga acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac
    12901 aatcaagtta ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac
    12961 tggagcacaa acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc
    13021 cggttggaaa ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa
    13081 actaactggt tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag
    13141 cagtggaatg ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga
    13201 tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc
    13261 attaggagag aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa
    13321 ttatgctgta gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga
    13381 agaggaagcg taaaatcata tcaacgattt cagggagtgg agccgattca aggcccactc
    13441 cctgaaatct gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg
    13501 aagtcatgga aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca
    13561 ccgttgaaaa attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg
    13621 atacagtaag attttaaaag taaaaagtaa gcatactttt ctaactctgt cttcaattgt
    13681 tttaaagaag tattcactaa tttatcttct gtagttgttt tgaccataac ttggtttttc
    13741 aaacctttga tactttcaat atataaaatt tcccgaggat acaggacgag acttccacct
    13801 ttttgatcaa taaagatttt atcgacattt acgatctgct gttctttcca attgatctgt
    13861 tctagcactt gacgaatttg ttcagataaa ttcctgtctt ttctgatata tccaatcgga
    13921 aggattttcc gattgatgat ctctactgca aaattcgtat aagcggtgaa aaaaacaata
    13981 ttactatgtg catttttttt acgtatttgt tcggcgacat caatacctgt gagtgattgc
    14041 ttcaagtcga tatcgataaa gaaaacatcg tgattatcaa tagtgcccaa ctggatcaac
    14101 tgattgattt gagagataga agtgagctgc acgatattct cgatatgatg attgaccaat
    14161 tctctgatga tccattcgct gtgaaactgg ttatcttcta tgataaaata ctttttcata
    14221 tgcaggctcc tattaaaaaa atatgtgtac attttgaaac aaaacaatgg aggaataaga
    14281 tggtcgcaac agtgacactt tatttgacct ttactttgat tttagattta ggtatctatt
    14341 tattcttttc gaaacaactg ctagtaaaaa acagctttgg ttgtatagct ttgatggtgg
    14401 tgacactttt acttcctatc cctaaagtga gctttcctta cctagttggc ttattattga
    14461 tgtttctttt ttattttact acgaaaaacc tgtttatcag ctgtttgatc agtaatcttt
    14521 tttttgctat cactggactc agcttttttc tggtttttga tctgatggat tatttaggaa
    14581 agagacatct tggaacagca agtttttttg cactacttat cgcatttatt ttctatctat
    14641 tagtaaaaag tatagacaaa cagacgagat ttctcaactt gctttttact tatccaactg
    14701 aacactattt tggaggtatt tgtattttat taagctgggg cgtgatttac ttggtattgg
    14761 gaagtgttca cttccgctca tctaattatc tgcttctatc tcttttgtcg atagtcatca
    14821 gttttttttc tttgtttttt tccattatgt tgatcagttt ccaagtaaaa gaaagacaac
    14881 atcaggaatc gcttcaactg tatcaaataa atgaagaata ttaccatcat ctagaggaat
    14941 tcaaacatga ttataaaagt ctactgtttt cattaaaaat tactcttcag gaaaataaaa
    15001 aagaaacgtt ggaattttta gaaatattag aagatcattc tgcagctatt tttgaaaaac
    15061 cgcaaataca acagctaaca aatattgtat ccccagctgt caggggtgtc tttttaaaat
    15121 ttctagaaaa agcagaatcg gagaaaatcc cgatccaagt agttattcct gtcgaagtga
    15181 aaacaatccc gattgatctg gttgttttta ttcgctgttt atctattttt atttctaacg
    15241 cattcgatca tcgtgtagcc gatcagagtc cgattacgat caatctgctg gaagaaaaag
    15301 agggatcacg ttttagtata gcgaacaagg caaatccaac gaatttgacg ctatcggaga
    15361 tggtcaaaag aggaaaaacc tctaaaaaaa atggcgggtt aggtctatac agtgcgaaaa
    15421 aaatgttgga tgtttacgaa aatgcagaac taaaaatcga attttcaaaa cagaatcact
    15481 atttttttgt agaaatgtat ctggcatcta gaaagagcaa atcagcctac tgacttactg
    15541 tcaaaggata agtttgagga aagtctacac ggagatagag acaacaacac agcatattca
    15601 aaagaaacag aagcgtgatg accgttgtca cccttctgtt tcttttgcta tgtagttaaa
    15661 caaagtagtt gacacttccg ctacttagta ttagtacgaa agtatacttc atgatactaa
    15721 gtagataaga agtgaaaagc ggaacccacg atcgtcaaaa tgctaactgc tttgcttaca
    15781 ccagataaag ggaagagcaa tatcgcttat tttaggcgtg gagaagcaag caaatcccta
    15841 cgatcacaaa aaaaatgctg acccagcagt taagagagtt agaacaagat gaaagtatcc
    15901 atcgaaaagc ctataaccaa gtcccgccta aagtagagta ctcgctcacc gaagaaggaa
    15961 aatcattaag agagattcta gtagcgttgt ctgtttggga agaagaacgt atcgaaaaac
    16021 aaaaagcctc aagggaagaa gacacgctac tgcatacaca tggtggctac ctaaattatt
    16081 gattttctat gacatttatt ttaaataaaa gaaaacagga gtttttttaa aagcatcatc
    16141 tattttttat gtaaaaaaat ggtatgatta atcaagtaag aagtgattgg aagaaggcaa
    16201 gcaaatgaat agtatggaaa aagtcaacca gtttagagat gaacgtaatt ggcgacaatt
    16261 tcataacgaa aaagatttgg ctatctctat ttctttggaa gcatctgaat tgttagaact
    16321 ttttcaatgg aaaacgccgg aagaagtaaa agaaacacaa ttagaacgaa tcaaagaaga
    16381 actggtagat gtattgatct attcatacat gatggcagac aaccttcacc tggatctaga
    16441 tgagatcatt gaagaaaaat tgatcaaaaa taatttgaaa tatcctgtag aaaaaagcgc
    16501 aggtgtacga aaaaaataca cagagttgta gaaatgtgtc attcggctgt tggaaagagc
    16561 aaaagaaaaa cagtaaaatg agtctggagg gagagaaaat gaaaaaaaaa ggaaaagcaa
    16621 aaagatggct agtcaatatc ctcttgttcc tcttgttatt agtaggacta gctttgattt
    16681 tcaatgaaca gatcaaggat tattttgtca gagagacagg agataaatat gctatagcga
    16741 atgtaacaaa agaagatctg aaaaagaata acgataaaga tgtcagtttt gactttgatg
    16801 cggtagaacc aatgacgaca gaaggggtca tgaggtcgca aatgagagga acagatctac
    16861 cagtgattgc tagtatcgca gtcccttcag tttcaatcaa tttaccggtt ttcaaaggct
    16921 tggataatac gtcactactt tacggagcag gaacattatc tccagatcaa gaaatgggaa
    16981 aaggaaatta tgccttagca agccatcgtg caacgaaccc tgaattgctg tttacaccgc
    17041 tagaaaattt agagatgggt gccaagatct atttgacaga tttagaaaat gtgtatacat
    17101 acaaaacatt ttttaaagaa aaagttgctc ctacagatac ccaattactg aatgaagtcg
    17161 agggaaaaga aattgtcaca ctaattactt gtggcgacat ggacgccgtc acgcgactag
    17221 ttgtacaagg agaactggaa agtgttacat cgataaaaga tgcgacagat gatatgagat
    17281 ctgctttcaa tctagagaca aaaacatttt aggatactgg gacataagtc gttacagatt
    17341 aaaaacagcc aaaggatatg aatcgataag gaaaaaccaa tttgctgaaa aaaagagaaa
    17401 cgcaaagagg tgcatctctg atttggttgt tttctagata gacttgtgtt ccagtttttt
    17461 tattttttat atgaaaaggc ttgcataaat aaaaattcga tggtatgatg attttgaata
    17521 agaaatggat tgttactgta cggcaggcaa aaccagaatt cagctaagaa acagtaggcg
    17581 aattctaggt ttttttattt ttcttgagag ggcgaagcaa aatgaaaatt gataaaattt
    17641 tgaataacaa tgtcgtcatt tcgaaaaatg gattcgggga agaagtcgtt tgtatgggaa
    17701 aaggactagc ttttcaaaaa aagattgggg acgagatttc gccagaagcg gtccaaaaag
    17761 aatttgtttt aagagattct tttgctaaaa accaattcca gcagctgatg gctgatattc
    17821 cggcagagga gatcgaatgg gtcaaacaag tagtcgaact ggctgaagat aaactaaaag
    17881 ttgaattttc gcctaatatt tatctgacat tgaccgatca tcttcattat gcgatcacac
    17941 gagcaaaaga aaatatccaa ctgcctaatc cattattatt tgaaacaaaa aaattttatc
    18001 cgaaagaata tcaagcagcg aaagaggctc ttttgttcgt gaaacaaaag acgggtgtgg
    18061 aactttctga ggatgaagcc ggttttattg cctttcattt tgtgaatagc caacaaggaa
    18121 acgaagacat gcaagtgacg atgaccgcta ccacaatggt aagagatata ctgagtatca
    18181 tcagtaaatt cttcggcatc gtgttcgatg aagaatcttt gaattatcag cgtatcatta
    18241 ctcatctgca gttttttgcc cagcgttatc tcaaagggga aggatctgac gagcaagatg
    18301 aatttctcta cgcattagtt caaggaaagt atccaaaagc gtttcattgt gtcgaacgga
    18361 tcaatgagta tcttcgacaa acgcaaaaac aggaaatggg gatcgcagag cagatttatt
    18421 taacgattca tattcaaaga gtagtaagtg agaaaaaaag catccaatca taaggattgt
    18481 tactgtatgg caggcaaaac ctggattgaa aaaataggtt cacgaatgtt tgtgactgtt
    18541 tttttcaatc tgggttttta ttttttaaat aaaaaagaaa gaaggaaaaa acaatggata
    18601 atcaagcagt tggtcgtcgt gtatgggaag cggtaggagg acaaaagaat gtcaaaagtt
    18661 tagtacattg tgctacgcgg ttgcgattca gattaaaaga tgaatcttta gcagatacgc
    18721 aaaagttaaa agaagacccg gacgtgatcc aagtcgtaca aagtggcggt cagtatcaag
    18781 tagtgattgg cagtaatgta gcagatgttt atcaatcgat cgtggatgag gaaggtcttg
    18841 cagtttcgga ggatggaaaa gaagagtcca aaaatatttt gaatcgtctg atcgatatta
    18901 tctctagtat atttacacct tttctagggg cgatggcagc cgccggggta ttgaaaggat
    18961 tcttatcatt ggcaacggtg atgggctggc tgacagctga tagcggtact tatcaaatat
    19021 tgtttgctgc agcagatggt gtctttacct tcttacctgt catgcttgct tttacagcag
    19081 ctaaaaagtt caaagccaac cagtttttag cagtagcaat tgctatggca cttgtgtatc
    19141 cagctatcac agcagttgct ggtgcgggag aagctatcag tttctttggg atcccagtga
    19201 ttctttcacc atctggttat acttcttctg tcataccaat cattctagcc gtatgggttc
    19261 aaagtaaatt agagccattc gtaaaaaaag tgattccgca atttctgcaa atgattttag
    19321 ttccgcttgt cgttttagta gtgatggttc cattaacatt tcttgcttta ggaccaatcg
    19381 ggacggttgc cggtaatgct ttaggcggtc tcttcaactc gatttatgga tttagtccaa
    19441 tcgtagcagg tctaatcatg ggaagtttat ggcaagtatt cgtgatgttc ggcatgcatt
    19501 ggggattcgt accgatcatg tttttgaata tcgaacaata tgggtttgat gtattgatgc
    19561 caatgcttct tccagccatc ttggcacaag gtggggctgc tttagctgta gcattgcgaa
    19621 caaaagatac gaaacttcga gcattaggga tttcttctac agtgacttcg ttatttggaa
    19681 tcactgaacc aactgtgtat ggtgtgacat tacctttgaa gaaaccattc attgccgctt
    19741 gtatttctgg aggtattggt ggagctatta tcggattttc cggagtaaaa gctttctcca
    19801 gcagtttggt cagcttattg acgattccga ctttcattaa tacggtggat ggtgtagagt
    19861 caaatgtgac agtagcagta atagctacag gaattgcttt tgttcttgca ttcgtaggta
    19921 cattgatttt aggatttgac gaacaaacac aagacaatca attagaaaac aaacatgcga
    19981 atgcaggaga accgattact tcagcacggc ataccttaaa aagtccttta acgggtaaag
    20041 tactgccatt gagtgaagta ccagaccaag ttttttcttc tggcgtgatg ggaaaaggta
    20101 tcgctattga tccagaagta ggagaactag ttgcacctgc agacggagag atcacgacta
    20161 tttttccaac tggacatgct gtagggatca caacgactga cggagcggag attttgattc
    20221 atatcgggat ggatacagta gaattaaatg ggaatggctt tgagatctta gtcaaacaag
    20281 gggatctcgt caaagcagga gatttattga ttcgttttga catcgaagca attagagcag
    20341 ctggctatag cgtaatcact ccagttgtca ttacgaatac tgatgcattt gccgatatac
    20401 ttgaattaga ccaaaaagag attattgcaa acgaagatgt tctggcaatt gtaaaataag
    20461 gaggaaatca ggatgcaatc acgtttttca gaaaaatttt tatggggtgg agcaactgct
    20521 gccaaatcaa ttagaagggg cttatgatcg agacggaaaa ggactatccg ttgcagatgc
    20581 tatgcctgga ggtaagcagc gctttgcgat tatcggcagt gaagaatttg actggacgat
    20641 tgatcaagaa aaatatattt acccgaatca tcgaggaatc gatcattacg atcgtttcaa
    20701 agaagatctt gccttgtttg cggaaatggg attcaaatgt tatcgttttt cgattgcctg
    20761 gacacgaatt tttccaaatg gagacgaagg tactcctaac gaagcgggat tagaatttta
    20821 tgatcagctg attgatgagt gcttaaaata tgatattgaa cctgtgatca cgatttctca
    20881 ttacgaaatg cctcttcatt tggcaaaaga atacggtggc tggaaaaatc gaaaactgat
    20941 cgacttctat gaacgatttg ctcagacagt attagagaga tacagcagta aagtcaaata
    21001 ttggatgact ttcaatgaaa tcaattcggc ctttcacttt ccagctttaa gtcaaggatt
    21061 agtaaaaagc aatggagcag gagagtacca aaatattttc caagcatggc ataatcaatt
    21121 cgttgcaagt agcaaagccg taaaaatcgg ccacgaattg cgttcggata tccaaatcgg
    21181 ctgcatgatt atctatgcaa caacttacag catcgatgcc aatccagtca atcaggcagc
    21241 aacaatgatc caaaaccaag aattcaattt tttctgcacg gatgtccaag tacgtggaga
    21301 ataccctgca tacacagcgc gtacccataa aaaatatgga gtcgatccgg aaaaattaga
    21361 gcaaacggaa aaagacttcc agttgctaaa agaatatcca gtggactaca tcggatttag
    21421 ttattatatg tctacggcaa tcaatgagac tgatccacaa gcagcaactt cagaagggaa
    21481 cttgctggga ggagtaaaaa atccgtttct tgaggcaagt gaatggggct ggcagatcga
    21541 tccagaaggg ctgagaatcg cattgaatga attatacaat cgttaccaga aaccattatt
    21601 cattgttgaa aacggtcttg gagccattga tcatatggaa gaggatggca cgattattga
    21661 tgattatcgg atcgattatt tacgtcgtca tatcgaagcg atggctaatg cagtagcaga
    21721 cggtgtagat ttgatgggct atacgccttg gggatgtatc gatcttgtca gtgcatcaac
    21781 aggagaaatg agtaaacgtt acggctttat ctatgtggat ctagacgatg aaggaaatgg
    21841 gacactgaat cgatcaaaga aaaaatcttt tgattggtat aaagaagtaa ttgcctcaaa
    21901 tggcgtaaac ttgtaattaa agcagctgaa tgaaaaagaa cacacttctc acatgtgatt
    21961 tgtgttcttt ttttattttt tatgtgctat tcttttagta atagtaaagc aaagggtgac
    22021 gttatgaaaa ggacacgtgt actatacttg gaagtcgcag ataaaattaa agaagatatt
    22081 ttctcaggaa aatatccagt cggttcgatg ttgccgacag aaagtgaatt agaagaactg
    22141 tttcaagtca gcaaaatcac tgtccgaaag gcaatcgaac tgctggcatc agatgaatat
    22201 gtcgcaaaga aaagtggtcg tgggacgacg gttctaagca atcgtcctta caatcgatta
    22261 tccaaagcgg caagctttac gcaaatcttg gagaactccc gatatgaagt ccggaaagaa
    22321 acgctggatt tgcaattggt ccatataaag aaggaagatc ctttgttttc ctttttcggt
    22381 gagcaagctg tttgttttaa gcgcttatat tatttgaaca atcagcctta tatttatttt
    22441 gttcattatc ttccacaaga aatgatgcaa ttcagcagag aagaatttgg caaagaatct
    22501 ttgtatcgtt tgctgacgaa aaaaggatat actattgact cattttctga tgatttctcg
    22561 gcagtatttt taacagaaga agaacaagga attctgaaaa cagacgcaca gatcggcatc
    22621 aaacggatac ggaaatcgtc tacgcaaatt ggggaaatga ttgaatattc ggaggcggtc
    22681 taccatacgg agctgcatcc ttaccatatt gattatgaaa cataaataaa aaaagaggtt
    22741 gtgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag cttctcatat
    22801 ttttcgtttg gttaggctta ttaccgcaga tacacggctg tttctggatt accatttatt
    22861 ctggataagg gggtgtggca cgattttcag aaggaatcta tgaaccaatc actgtattga
    22921 ttttagcttc gatggtaacc ccattcggct tgacgatcgc ttatttctta gggaaaatca
    22981 ttcgtaaaaa tatactcaat cgtcaagaaa tcgatacatt gaagaccgct tttcctatgg
    23041 ggatctgtca gatcacagag ggatgtttcc ctatcgtatt gaacgacttg gcacgcaatg
    23101 tcatagcaac gggagtcggt gggacagtag gtggcggatt aagcatgttt tggggcgcag
    23161 atagccgtat tccggcatcg ggaatgtttg cagtcgctac gatgaccaga ccatgggcat
    23221 tcattggtgc attgttagct ggttcttttg tcacaggaat aaccatttta ttattgaaaa
    23281 aaccagtaga tccgaatgca gaaatcatcc aagaagaaaa agaggaagaa gacatatctt
    23341 gggatgattt gacaattagt taggagaggc agaatggatt acaagaaaaa agtacaatag
    23401 atgtaccttg agacaggaat ggtatgaaag aatcagagtt agaaagaatc gaatatacag
    23461 attttggttt aggagcgatc gaaactgtcc agagcatcgg caccctcctt gaaatgacga
    23521 tgaaggaaaa atgagaacct ttcgttgtcg aaagggtctg gtatacctta tgtcgaagga
    23581 aaggaaacaa aaaaactttc ctgtactatt ccaaaaagga aagaaagtta ttatacagta
    23641 aaaaaagaaa ttattcttca tgctagagat cagtacacgt tcgagcccaa tatcaaacac
    23701 tagttccagg caggcgcaac aggtgcggtt atcactgaat tttcttccag cagtgacgat
    23761 tcaagtgata tttttacaaa tccagctatt tatcgttaac aaaaagattt ttaggagaag
    23821 ctgatgaaca aaaatgatca aattttgatc gtttagttcg tcggcttttt tatttagaga
    23881 aacatcattt atcagtgaaa cataacacga gaacaaaatg attttttcgt taataaaaga
    23941 aacaagtatt tgttgtttaa aataatacac gttattggca tataggacga aatcccagta
    24001 tcttctataa gtaagtcatt tcatatcgat gaaaacacgt tatttatttt tgttttatct
    24061 ataaaatggc tgaaaaatga tttttttcta aatactatat atagtggcgt ctatcctata
    24121 taccactaaa aaactacaaa atctagtctt ttattttaaa aaaacacgtg tagaatgaag
    24181 atagactttt gagttaggga gtgttttttt atgaaactta ctgttttttg cggttcccgt
    24241 tttggtaata aagaaagtta caaatttatt gcgcaaacgt taggaaaata catggcacaa
    24301 gaagaaatcg aactggttta cggtggttca tactccggga ttatgggagt gattagtaaa
    24361 acagtactgg aaaataacgg aaaagtcaca ggtatctatc cgaatgggct ttttgaaact
    24421 gaattgcctg gaaaagatgt tactacattt attcctacag aaacgatcga tgaaagaaag
    24481 gtattattat ttgaaaaggg agatgctgtc ctcgttttcc ctggaggtct aggtacattg
    24541 gaggaattct cacaacttct ttcttggata gcaatcggtc tgacgcccga caagccaatc
    24601 ggaatattga atattggcgg ttattacagc ggcttgcaac aattattaga aacttttgca
    24661 aaagaggatt ttatggataa aaaatggttg gatcaagtaa tcttttccaa caatccattg
    24721 gaactggtca atgaattacg agcagtatct attaaaaatc agttattatt gaaggaggca
    24781 aaataatgag tcaaatatat ttagcaggac catttttttc agaggagcaa atcgatcgag
    24841 taagtcgtat cgaaaaagcg ttggaggaaa acaaaacagt cacaagtttc tactctccac
    24901 gtcatcacca agaaagcaat tatgaactat tcagcgctgg atgggcgcag gaagtatacg
    24961 aaaaagatat ggaagaactg acaaatgcag aatttgttgt tgctatcctt gactttgagc
    25021 atcaaacaat cgatccagga acggcttacg aattaggtgt ggcaactatg ttgaaaaaac
    25081 cgatgatcat tgtacaagaa gagacggtac ctacgaactt gatgattaca caaagtctcc
    25141 atacgtattt aaaatcagac caagctgtac gtgaatatga ttttgaaaca ttgcctgttg
    25201 aaacttacgt aggcgagtac ttatagaaga ggaagggttg tatgtcagtg attttgattt
    25261 caggaacgat tggtgcaggt aagagcagtt tgacggatat gcttgcaaaa gagattgatt
    25321 ctaaaccatt ttatgaaaat gtcgaagata acgaagtatt gccgctcttt tattcaaatc
    25381 ctgagcagta cgctttttta ttgcaaatct tctttttgaa caagcgattt ttagcaatga
    25441 aaaatgcttt agtgaacgat gacaatgtgc tagaccgatc catctatgaa gatagcctgt
    25501 tgttccatct aaacgctgat ttaggtcggg taacggatat cgaagtacag cagtacgata
    25561 gcttattaga tacgatgcta aacgagttag atgatgttgc tccaaaaaaa cggcctgatt
    25621 tgatggtcca tatcaaagtt tctttggaca cgatgctaga acgtatcaaa aaacgtgggc
    25681 gtgactatga acagctagaa agcgatgaga cgttatatac ctattatgaa acgttgaaca
    25741 cccgctataa tcaatggtat gaagattttg atgtatgtcc taaaatacaa gtggatgggg
    25801 ataaattcga ttttgtagaa aatccagaag atgcaaaaaa ggtgatccgt cagatccaag
    25861 aaaaattagc tgaattggaa ggaaagcctg ctggaaaata ttttacggat aaccagcatg
    25921 caaaaggtca agaattaggc taaaaataaa aaaccaaacg acgggaaaga aaaattacta
    25981 cgtgatgtag aataagcctt tctatcgttt ggttatttta tattcaaata tttgaaataa
    26041 ataaatcttt actctttgat ttgtaaactg aaatgatcca ctgtttgttt tactgatgaa
    26101 gcttgatcaa aggaagaagc tgagtttgaa gttcataaaa atcagccata gtgagttttg
    26161 cttggatttg tttatcttct tctacttttt acataatttt gaacaggata agattcaaat
    26221 gttttaaatg aatctttgac tagtttgtga cagttgtcgc atatgctcaa gcttccttgg
    26281 tcattaaatt ctgtatggat atttttgttg ttattacatg tacaataatc aggactatcg
    26341 atcatccatt tcgtctcctt tacaatcatc ttgcttcttt actatagcag aaaagaaggg
    26401 gagaaacaaa agaagatcca acagacggca ctatagactg tagatgttgc catcctcatc
    26461 cttcaaacgg taattaccga tttttgtaaa tatgactgtt gtttcttttt tattttcttt
    26521 tcctgaaata atatattcgt catctttggt agtgatcttg atatctgtca gcactgtttt
    26581 ttcttcagat gattcagaag aatcagttcc ttgtgtctga tacgtgcgtg tccattccat
    26641 ttttttatct gtaaatgtca actgtcccgc ggataaatcc atatctttac gtacttttag
    26701 cgtatataca ctgttggtgg tatctgcttt ttccgctttt aagacagaag aatcggttgt
    26761 ttcgtccgag gattggtcag agccgccaaa caaatcacag ccagtcaatg tcaaaagtcc
    26821 cgcagacaaa ataaatagta cgattaattt cttcaaaata cttcccccat ttgataggat
    26881 aaagaaatta taccattgga atgtaaaact tcaaacggat ggaagagaaa aacgcacaaa
    26941 acacccacaa atagagtaaa ttttagaata aaattttaaa aaggcaaaaa atccgccact
    27001 ttcagaaagc gttttaaaaa agtgttgcaa tcttttaaaa aaatgctata ttaaaaatga
    27061 accggttcaa atacttgttt aagtataatt aatagtgaga ggaagttgct ctactgaaga
    27121 ctttagttat atggatattg atgacaagca acactatgca aagaatgcgg tcatcataat
    27181 cgaacaacaa gaaagagaaa tcaaatgtaa ctggcaggag tgagaatgta tggcatcgat
    27241 ccgtgatgtg gctaagatgg ctggtgtttc agtgggaaca gtttcccgtt acttgaatgg
    27301 tcagcaactg aaggaaaaga acatgaaaaa aatagcagaa gcgatttcag cgttggacta
    27361 taaagaaaac atcattgcta aagggttgaa gaataatcga agcttctcta ttggactttt
    27421 gataaacggg atttccagcc gttttgggtc ggaagttgtc tcagggatcg aaagagtagc
    27481 tgaagaaaat gggtatagct tattattaag cgggtttgcc gatcagccag aaaaaattga
    27541 acaaaaaatt gaatacctga tgaagcatgt gatcgatggg ctgattgtct tcttatccga
    27601 agaagagtgg tctggatttg aaatgctcac aaaaatgagt attccagtta tcgcattgaa
    27661 ttgtccagaa ggaccagcag gtattgatac aatattagtg aatgatcgag aaagtgtagc
    27721 aaaagtgatc gctcatcatg gtgaacaagg acataaaaaa atcggattta ttgctgccac
    27781 tcaaacagac tacgtagcaa gagaacgttt agcaggagcc aaagaagcgg tccaaaatga
    27841 tccatcgatc caattagagg tctttacagg agactattct agaaaaagcg gctattacgg
    27901 ggcaaaagca ttattagaaa aaggggcaac tgctattttt gtcagcaatt acaatatgtc
    27961 gattggtgcc atcgaatgtt tcaatcaaga aggtgtcaga atcgggaagg atattgcgtt
    28021 cagtcactat gattatttag ataaaaacga tctgagtatc cttccgaaaa tcacgatcac
    28081 gccaccaaca gataaaatcg gggaacgagc agcagaacag ctgttaaaac gaatcaaaaa
    28141 agaagaggaa gaaaatgaaa aagtgattgt gatggaaaat atcatacatg gaatcaacta
    28201 aaacgaacta aaaaagaaaa agaggtgctt tgatgaaaaa atatcaattt ccagcaaatt
    28261 tttggtgggg ttctgcagca agtggcccac aaacagaagg aaggatttca ggcgatggaa
    28321 aaggagagaa catttttgat tattggtaca aaaaagaacc gcaaaagttc tttgatcaag
    28381 taggacctga aaaaacctcg caagtttata cgaagtatca agaggatgta cagttgatga
    28441 aacagactgg gcataattca tttcgaacat ctatccaatg gagccgtttg atacctgatg
    28501 aatctggaac aatcaacgaa gaagccgttc gtttctatca tcgttatttt gatgagttga
    28561 ttgaaaacgg tatcgaaccc tttgtgaatc tgtatcattt cgatatgccg atgtatctgc
    28621 aagaaaaagg cggatggttg aaccgagaaa cggttgaagc ctatgaaaaa tatgccaaaa
    28681 gttgttttga attgtttggt tcgaaagtaa aaaaatggtt tactcataat gaaccaatcg
    28741 ttccagtcga agcaggttat ttatatcgat ggcattatcc agaagagtca gatatgaaaa
    28801 aagcgatcca agttggctat catgaagcgt tggcttcagc gttagccatc aaatcttacc
    28861 atgaaatgga gcaaggtggt gttattggta tcatcttgaa tctgacacca agctatccaa
    28921 gagacgaatc aaaccctgaa gatgttaaag cagcgcaaat agcagatgcg ttcttcaatc
    28981 gttcattcct tgatccagct gtaaaaggcg agtttccaag tgaattgatt gagatagtaa
    29041 aagaacttga tctcatgcct gtgatccatg cagaggattt gacggttatc aaagaaaata
    29101 cagtagatct tttaggtatc aattattacc agccacgccg ggtaaaagca aaagaaacac
    29161 caatcgatac taaaaacggt ccaatgcctg aggattattt cgacaattat gaaatgcctg
    29221 gtcgtaaaat gaatccttat cgcggatggg agatctatga acaaggaatc tatgatattt
    29281 tgatcaatgt acgagatcat tatggaaata tcgactgctt tatttctgaa aacggtatgg
    29341 gtgtagaaaa cgaagcacga tttatcaaag aagatggcat gatccacgat gattatcgga
    29401 tcgaatttgt ccaatctcat ctgaaatatg tccatcaagc cattcaagaa ggtgccaatt
    29461 gtttaggcta tcatatgtgg acatgtatgg ataactggtc gtggaccaat gcatataaga
    29521 atcgatatgg ctttatttca gtagacttag cacaagatgg aaaaagaaca atcaaaaaat
    29581 cgggtcattg gttcaaagac gtatcagata aaaacggatt tgaggcttaa gaaagaatag
    29641 ttgagagcaa atgcgtgaaa ttctccattc ttaccatttt gtgaaaaaga agttcaatta
    29701 tttacgatct ttttcaactt ttttgtcttt ctatttcatt ttttgtatat aatagaatag
    29761 tcaaaattaa ataggagtgg aggaaagcat gaggaaaaaa agaccgtttc ttctcttttg
    29821 gttattcggt tttttcttac tgcttatttt atccgcagca ttttcaggaa aaatgacagc
    29881 agatcttgct cagaaagaaa cagaaaaaat cgtagcaagc aaagaatcag tagataattt
    29941 caaaaaagaa ttctcaactt attggtgggc ggagtataaa ataactgcca attacgaaaa
    30001 aggcgaattg gacatttatt tgccaaaagg aaaagaagaa aatcaattta ttaatatcga
    30061 tgacgtactt agtgcgattt ctctcagaca atatcaattt aaagacaagc atttgaccgt
    30121 tttgcttttg actcatgatg gcgacaaagt agcccgtttg aaaaatggaa aagtgatcac
    30181 cgcttattct gaaaaagtag acaaaaaagt ggtcgccgat cgtttagcgg aatggaaaaa
    30241 gacaaatgaa caagtcaaga aaacaactgt ctcttctcaa aaaaacgagt cgacaggaac
    30301 aagtccttca gattctggca gtggacaaac gataacggga agtgaagaaa atcaagcctc
    30361 aattattacg aatgagtcat cacaaggaca atatcaatcc tatggacaat gaaaaacaga
    30421 ggttgtg
//
LOCUS       DACCCS010000032        30351 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.032, whole genome shotgun sequence.
ACCESSION   DACCCS010000032 DACCCS010000000
VERSION     DACCCS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.032"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            172..516
                     /locus_tag="IVK82_001496"
     CDS             172..516
                     /locus_tag="IVK82_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP6392566.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="IVK82_001497"
                     /pseudo
     CDS             517..936
                     /locus_tag="IVK82_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="IVK82_001498"
     CDS             complement(1028..1378)
                     /locus_tag="IVK82_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392567.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="IVK82_001499"
     CDS             complement(1344..2675)
                     /locus_tag="IVK82_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP6392568.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="IVK82_001500"
     CDS             2931..3497
                     /locus_tag="IVK82_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6392569.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="IVK82_001501"
     tRNA            3642..3729
                     /locus_tag="IVK82_001501"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /locus_tag="IVK82_001502"
     CDS             4063..5037
                     /locus_tag="IVK82_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP6392570.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="IVK82_001503"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="IVK82_001503"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP6392571.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="IVK82_001504"
     CDS             complement(7452..7898)
                     /locus_tag="IVK82_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP6392572.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="IVK82_001505"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="IVK82_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP6392573.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="IVK82_001506"
     CDS             8363..8881
                     /locus_tag="IVK82_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6392574.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="IVK82_001507"
     CDS             complement(8958..9914)
                     /locus_tag="IVK82_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6392575.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="IVK82_001508"
     CDS             10080..10886
                     /locus_tag="IVK82_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6392576.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="IVK82_001509"
     CDS             10883..11872
                     /locus_tag="IVK82_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP6392577.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="IVK82_001510"
     CDS             11869..12873
                     /locus_tag="IVK82_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP6392578.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="IVK82_001511"
     CDS             13005..13547
                     /locus_tag="IVK82_001511"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP6392579.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="IVK82_001512"
     CDS             13567..14265
                     /locus_tag="IVK82_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP6392580.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="IVK82_001513"
     CDS             14286..14498
                     /locus_tag="IVK82_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP6392581.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="IVK82_001514"
     CDS             14498..15460
                     /locus_tag="IVK82_001514"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP6392582.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="IVK82_001515"
     CDS             15478..15873
                     /locus_tag="IVK82_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP6392583.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16036..16401
                     /locus_tag="IVK82_001516"
     CDS             16036..16401
                     /locus_tag="IVK82_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP6392584.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16398..18209
                     /locus_tag="IVK82_001517"
     CDS             16398..18209
                     /locus_tag="IVK82_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP6392585.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18271..18438)
                     /locus_tag="IVK82_001518"
     CDS             complement(18271..18438)
                     /locus_tag="IVK82_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP6392586.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18686..19675
                     /gene="galE"
                     /locus_tag="IVK82_001519"
     CDS             18686..19675
                     /gene="galE"
                     /locus_tag="IVK82_001519"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP6392587.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19687..21171
                     /gene="galT"
                     /locus_tag="IVK82_001520"
     CDS             19687..21171
                     /gene="galT"
                     /locus_tag="IVK82_001520"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP6392588.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21195..22175
                     /locus_tag="IVK82_001521"
     CDS             21195..22175
                     /locus_tag="IVK82_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6392589.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22264..23088
                     /gene="tsaB"
                     /locus_tag="IVK82_001522"
     CDS             22264..23088
                     /gene="tsaB"
                     /locus_tag="IVK82_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP6392590.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23072..23614
                     /gene="rimI"
                     /locus_tag="IVK82_001523"
     CDS             23072..23614
                     /gene="rimI"
                     /locus_tag="IVK82_001523"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP6392591.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23621..24085
                     /gene="rimI"
                     /locus_tag="IVK82_001524"
     CDS             23621..24085
                     /gene="rimI"
                     /locus_tag="IVK82_001524"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP6392592.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24082..25098
                     /gene="tsaD"
                     /locus_tag="IVK82_001525"
     CDS             24082..25098
                     /gene="tsaD"
                     /locus_tag="IVK82_001525"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP6392593.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(25707..26408)
                     /locus_tag="IVK82_001526"
     CDS             complement(25707..26408)
                     /locus_tag="IVK82_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6392594.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            26869..28098
                     /gene="arcA"
                     /locus_tag="IVK82_001527"
     CDS             26869..28098
                     /gene="arcA"
                     /locus_tag="IVK82_001527"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP6392595.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            28189..29208
                     /gene="argF"
                     /locus_tag="IVK82_001528"
     CDS             28189..29208
                     /gene="argF"
                     /locus_tag="IVK82_001528"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP6392596.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            29323..30270
                     /gene="arcC"
                     /locus_tag="IVK82_001529"
     CDS             29323..30270
                     /gene="arcC"
                     /locus_tag="IVK82_001529"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP6392597.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
BASE COUNT         9921 a         5198 c         6318 g         8914 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaacga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggacgggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaaaaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattgttcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acatacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcag ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcata tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt taagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ttcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg atcttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaatcg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcaggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgcat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt tttttatttt gtgctattat ctacatatat aagctaagcg aaaaggtaga
    16021 aagaaggaaa caactatgat caaattaaaa cgtgtttacc ttgatgctga aaaagaggat
    16081 ggctatagaa tactcgtgga tagattctgg ccacgaggaa tttcaaaaga aaaagcgcag
    16141 ttagatctgt ggttgaaaga aattgcacct agtaatgaag cacgcaaaac ttttgaccat
    16201 gtaccagaac gatttgcttc atttgcaaag aaataccagc aagaattgat tttcgaagaa
    16261 gaaaagaagc aagctatttt taaactcagt caacttatga aagaacatcc agtcattact
    16321 tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg ttttattgaa aaaatttatc
    16381 gaaatggagg catgcaaatg aaaataggga tcattggtgc cggacctaga ggattaagtg
    16441 tagcagaaag gattctccat aataatcagg acagacattc tcttgaactt tttcttttcg
    16501 atcccaatgg tcctggaggt cgagtttggc ggttgaatca gccaccggag ttattaatga
    16561 attctgtttc tcaacaagtg acgcttttta cagatgaaac attgtccagt ggaggattgg
    16621 taacacaggg acccaacttg tttcaatgga gtaaaggacc tgctacagat tatatcgaac
    16681 agacggtaac caaaaataag caactaatta tagaagaaat caatcgtctg gaaaaagatc
    16741 agcaatctac tcgatgttta tacggactct atcaacgctg gtttttctca aagttgcaag
    16801 aagaatttcc agcttctatt cagtttgttt attctttagt aaaaaaagta agcaaaagaa
    16861 aagatggtta tcttttacag acagatcagc aaagctattc tatcgatcag cttgttttag
    16921 caagcggtca ctgggaaaat gatgagacag aagaagaaag agtgtgggta gactacgcaa
    16981 agaaaaatgt tttactgtat cagcttcctt ctaatcctgc agatgtagat ctttcgaaga
    17041 taccgcctaa agaaaatgtc tttttaagag gtctaggctt aagttttttt gattatgtcg
    17101 gattgtttac agtatcaaga ggagggcgct tcgagaacga aaagggaaaa ctggtctatc
    17161 atccatcagg caaggaaccg attgtataca gtggatcaag aagggggctc ccttattacc
    17221 ctcgaggaag aaatcaaaag caaggcggag caatggcatg gccacggtta ctaacaaatg
    17281 aaaacctgga gcaatggcat cagtcgggat ttttgactgg cgaaacattt tttgactatc
    17341 tgaaaaaaga cgttgagttg ttttattaca agaaaatcat cgaagaatat cacttgccga
    17401 ttccaaagaa aaattttgag catgattttc tctccctctc ttcagaggag tgtctcggaa
    17461 agcatccagc actaatccct tatagatggg attggtcgta tttagaaact cctcttgcct
    17521 atcagaatga gcccttttca gaagctagcc gaatatttat tgaagggcag ataaaagaag
    17581 cggaaaaagg caattgcaca ggagcatttg cttcaacatt tgatgcatta aaagactggc
    17641 gtgatccaat ccggcaggct attgaatggc acgttttttc tgctaaggaa tacaaagaaa
    17701 tactatgggg atggtttact catctaaata gctttttaac gatcggtccg ccaatcattc
    17761 ggacgaaaga attagcagca ctgatagatg cgggtatttt tcatttgatc gaaccgccat
    17821 ttgatattca gcaaaagcac aaccgctatg ttatttctct acaagataaa gaaatcagca
    17881 gtcgctattt gattgaagct cgattgccac aaacaagctt ttctcgttca agtaatcctg
    17941 tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt ttcattttca gataatacag
    18001 gaagttatca ttcagaagct atagatgttg agcgaggtac gaatcgcgtg attgatgctt
    18061 caggggaaat ccagtctgac ttttattgct atggaatacc tgttgaaggt gtggattggc
    18121 taactgcagc agtagctaga ccttatacag atccatggaa tctgagacag gcagataaaa
    18181 tagcacaact tatattgact gacaaataaa aatacgcatg atgacttatc tgttttttag
    18241 aaagtcttca tgcgtatttt taaataatta ttaacgagaa acgcgtttac gcattgcttt
    18301 tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt ggatcgatca ctgttttttt
    18361 cacagaagca accaaaccag caacggcagc ggctgttgcc agacttccta ctaatattcc
    18421 agatacaaat tttttcattg aatccacctc atttattatt tgttatatct attatggaac
    18481 aaaagagaaa aaaaggctag ttttacgttt gtttgtaaac ctttgcaaaa aatccttgaa
    18541 aatgtttact aaatgtttta ttataaattt accatatgaa tttacgaaat tttactttgt
    18601 ttgttttcac cataaaagta aacttcgttt tttaaattat atagaatcaa caaacacaat
    18661 tttactaatc aaaggagtgt tttttatgac aattttagta ctaggcggag ccggttatat
    18721 cggttcgcac gctgtagacc agctagttca aaaggggtat caagtagctg tggtagataa
    18781 tttgctcaca ggtcacaaac aagcagtaca tccagatgct catttttatg aaggagacat
    18841 ccgggataaa gaatttctac gttccgtatt tgaaaaggaa ccgattgaag gagtaatcca
    18901 ttttgctgca agctctttag taggagaatc tgtcgaaaaa ccattgatgt atttcaacaa
    18961 caacgtatac ggtatgcaaa tcttacttga agtcatgcac gagttcaacg tgaataaaat
    19021 cgtattctct tctactgcag caacttatgg agagccgaaa gagtctccaa tcacagaaga
    19081 tacaccagct aatcctaaaa atccgtatgg tgaaagtaaa ttgatgatgg aaaaaatgat
    19141 gaagtggtgt gaccaagcat atggcatgcg ctatgtcgct ttgcgctact tcaatgtagc
    19201 cggagcaaaa gcagatgcat ctatcggaga agatcataca ccagaaacgc atcttgtccc
    19261 aatcatttta caagtagctc ttggtcaaag aaaagccttg gctgtttatg gagacgatta
    19321 tgatacacca gatggaacat gtatcagaga ttatgttcaa gtagaagatt tgattgctgc
    19381 acatatttta gctttggaat acttgaaaga aggaaatgaa agcaacttct tcaatttagg
    19441 cagcagcaaa ggatactcag tcaaggaaat gctagaagca gctagagaag tgacaggaaa
    19501 agaaattcca gcagaaatag caccaagaag agctggagat cctagccgat tagtcgcttc
    19561 aagtgaaaaa gcgagagaga ttctgggctg gaaacctgaa tacacagata tcaaagcgat
    19621 tatcaaaaca gcatgggatt ggcatgtcag ccatccaaat ggttatgaag aataggggaa
    19681 tgagatatgt caatcagtca aacgatttgt gattttgcca cacttgcgat cgaagctggc
    19741 ggatggatgg aaatggaccg cttatattta cagaatcgtc ttttagggat ggtaggagaa
    19801 gatgctctag aaagcattga agtaagacca gttaaggaag aatctgtaga cttattggat
    19861 cagcttgtcg ctaaggcaag agaaaatggt gttctttcag aattgccagc agaacaagag
    19921 atttttgaag cgcaattaat ggattttctc acaccgccac cttcagtcgt caatgcattc
    19981 tttgcacagc actattcgaa aagcccagag gaagcaactg agtatttctt taatttaagt
    20041 aagaaaaatg actacatcaa aacaagagcc atcgcaaaaa acatcaaatt tccggcagag
    20101 tcggagtatg gtgaactgga aatcacaatc aatttatcta agccagaaaa agatccaaaa
    20161 caaatcgcag cagaacgcga ggcggtaaaa gtagactatc ctacctgcat gctttgcatg
    20221 gaaaacgaag gatatagagg tcgcttgaac tatccagccc gcaccaatca ccggattatt
    20281 cgaatgaatc tagacggtga aagctgggga ttccaatatt ccccttatgc ttattacaat
    20341 gaacattcga tcattttatc agaacaacac cgcccgatga aaatcacaaa ggaaacattt
    20401 gctagactgc ttcggattac tgaagtcttg cctcattact ttgttggttc gaatgctgac
    20461 ttaccaattg ttggaggatc gattctttct catgatcatt atcaggcagg acgtcatgaa
    20521 tttcctatgg caaaagcacc gatagaaaaa cttatcaaac tagaagatta tccgcaagtc
    20581 atcgcaggag tcgtgaaatg gccgatgtct gtgatccgtt tgcaatcaac agagaaaaac
    20641 ttgcttattg aagcagctaa ctcagtattg gaaaaatgga aagcatattc ggatgaaaca
    20701 gtatctatca gagctttttc agcagatggt acaccacacc atacgattac gccaattgca
    20761 cgaagaaggg acaattactt tgagttggac cttgtccttc gagacaacaa tgtttcagaa
    20821 gaacatcctg atgggatttt tcatccacat aaagatgtcc aacatatcaa aaaggagaac
    20881 attgggttga tcgaagtaat ggggcttgct gttttaccac ctcgtttgaa accagaacta
    20941 gtagaagtag agcatttctt attaggagaa gaaaaccaga tggctgaata tcatcgtccg
    21001 tgggcagaac aattaaaaaa agaacatcct gatttgacga aagatacggt caccgatgtc
    21061 gtacaaaaat ctgtaggtca ggtttttgca cgtgtactgg aagacgctgg agtattcaag
    21121 cgagatgaaa aaggacaggc agcgtttgaa cgatttactg cgattttata aacgtaaaaa
    21181 aggaggagag acaaatggca acaattaaag acattgcgca gttggcagga gtttctcctg
    21241 ctactgtttc aagagttctc aactatgatc ctgatctttc cgttggctta gccacaaagc
    21301 aaaaaatttt tgaagcagca gaacgcttaa attatacgaa acatcgtaaa aatgcagcaa
    21361 atactaagca aacgcttcgc ctgatccagt ggtacgacga tacggaagag cttgaagatc
    21421 tttattattt atctattcgc ctaggaatcg aaaaaaaggc agaagaagca aacgttactt
    21481 tattgaaaga aacgtgggat tccattagtg atcttccggc agatggaacg atcgcactag
    21541 gaaaattcga cgaagctcag ttattagaaa tgaaaggatc ccaaaatgcc ttgcttttgg
    21601 tagattctga cggcacttct caaggaatcg actctctagt tgtcgacttt aagtcaagtg
    21661 ttcaaaaagt cattgaacac tttgtctccc ataaagcgca acatatcgct atgcttgctg
    21721 gaacagaata tacgaagaaa aaccatcagc ggctaaaaga tcccagaatt atggcatttc
    21781 aacaagtttt ggcagaaaaa ggctacgacg aaggtccaat tatcgaagca gatttttctg
    21841 tcgagtcagg atatcaagca gtgaagcagt atttaaaaac gaatccgcat gttcctgatg
    21901 cgctttttgc agctaatgat gcattagcga ttggcgcact gaaagcgatt caagaaaaag
    21961 gtttagtggt accagaagat atttctgtga tgggcttcaa tgatgtcagt gtagcgaaat
    22021 atgtaacgcc ctcgctttct actgtaaaag tttataccga atggatggga gagctagcag
    22081 tggagacgat tcttcaatta agtaattcca aagcaccggt ttcaagaaag attattgtgg
    22141 aaacagagtt gattattcga gaatcaacga aataaaagat ggaaaagctg tgggaaaatg
    22201 gagtgatacc gcagcttttt tgtgtttcag gaaggggcaa aatttgcccg ccagcagata
    22261 atcatgtatt attgtactag ttttgccttt ggtattttca tcctcagaat aaaaaagtat
    22321 gctacaatgg aaggcgagaa tgtcatagca gaaaggatca aatatatgat tacattagga
    22381 atagatactg caaaccaaac tttggcgatc ggtgtggtag aagacgaaca aattcttgga
    22441 caaatacaaa caaatatcaa acgcaaccac agtgtaacac tgatgcctgc aatcgaccaa
    22501 ttgtttgcag atcttcaaat aagtccaaaa gacattgacc gaatcgcggt ttctgatggt
    22561 cccggttctt atacagggct tcgtataggt gtaacgacag caaaaacgat tgcttataca
    22621 ttagataaag agctagtagg agtatctagt ctgaaaacca tcgcggctaa ttgcatcggt
    22681 gtggataata ttattgtgcc catgtttgat gctcgaagaa aaaatgtata cgctggggca
    22741 taccgctgga aagaaaatag attagaaact gtacttgaag atgtacatat ttctgcttct
    22801 gatttgttca cacaactaca aaagctgaat gaagctattt atttcgtcgg gagtgattgt
    22861 cacaaattct tctctgaaat aaaggaaata ttgcctagtg cgatgattaa tacagttcca
    22921 gcatgggata ttccaagtgg tgtaactgtg gctcaattag gtgctttagt tgaaccagtt
    22981 gcagagaaac aagcttttct tcctcgctat ttaaaacgag tagaagcaga agaaaaatgg
    23041 ttggagacgc acacacctgg agacgaaagt tatgttgaaa aaatttagag cgtatgccag
    23101 aagctttttt caagataaaa tgatttatac agaaaaaaca gtcgaacaaa tcgatcgtcg
    23161 ttacgtacta agagaattga ctacagaaga tatcaaagag ctgctggatg tcgaacgaga
    23221 agtctataat ggcgaattgc catggacaaa aagtgccttt atatcagaat tgaagtcacc
    23281 gcttcctcat ctatatctag gtatctttga taatggaaga atgattggtt ttatcggttc
    23341 aagagcgttc ggtttagatt gccatatcac gaatattgcc gttcgaccgg catatcagcg
    23401 taaacgaatc ggttcgctgc ttattgacga aatcgagaac tttgctataa tgaatcgctg
    23461 tgagacgatg tcattagaag tgcgtatgag taatcaagat gcacaaagac tttatcggaa
    23521 attggggttt caagcaacaa cgatcaaaaa aagatattac actgaaacaa aagaagatgc
    23581 gttggatatg ataaaacttt tagaaaatga gtgagattag ttgatttgca gaaaaaaaga
    23641 ttttgatgaa gcgtacttgg cagaatgcgt atggatgatt agcgaaaatg cttatccaca
    23701 tggttcacct tggacgaaaa tgcagttttt ggcagacatc caacagcctc actctgatta
    23761 tctgctttta gctgaagata accagatctg tggctttatt ggatactcga aagtaataga
    23821 tgaagtcgag atcacaaata ttgctgttgc agtttcagaa caaggaaaag ggcatgcacg
    23881 gcatttgtta caattactga ttacggaaca aataaaagat tcgctgcatg tttttttaga
    23941 agtacgtcta tctaatgaag cagcaagaaa actttatgaa tcagaaaagt ttcgtatatt
    24001 aggtaaacgg aaatcatatt atcgcaatcc gacggaagat gcgatcatta tgagcagaaa
    24061 gttaaaaacg gagacgatga aatgaatgaa gaattgattc tggcgattga atcaagttgt
    24121 gatgaaacga gtgtagcggt cgttagaaat ggaacagaga tcttatctaa catcgttgct
    24181 tctcaaataa acagtcataa acgttttggt ggcgtagtac ctgaagtagc tagccgccat
    24241 cacgtagaac aaatcacctt atgcttggaa gatgcactgg tagaagctgg tgtgtcggca
    24301 gaagatttat cggcagtcgc tgtcacttat ggaccaggac ttgtcggctc gttgctgatt
    24361 ggtatttctg cagctaaggc ttttgcttgg gcacaccaat tgccgcttat tcctgtaaac
    24421 catatggcag ggcatatcta tgcagcccgt ctggttaaac cattccagtt tccattgatg
    24481 gcacttcttg ttagcggagg acatacagaa ttagtctata tgcaagaaga tggatcatat
    24541 gaaatcattg gggagacaag agatgacgca gcaggagaag cttatgacaa agttggaaga
    24601 gtattaggtc tttcttaccc aagcggaaaa gaaatcgatc agctagctca tcagggaaaa
    24661 gataattacc attttccgcg agcgatgatc catgaagata attatgattt cagttttagc
    24721 ggattaaaaa gtgcatttat caatttggtg cataatgcac aacagcgtgg agaagatcta
    24781 gacaaaaatg atttagcagc tagctttcaa gcaagtgtga tcgatgtatt gattactaaa
    24841 acacttcgag cttgtcaaaa ttatccagtc aagcaactga ttatagctgg aggtgtagcg
    24901 gccaatcaag ggttgcgtga aggattgcag gatgctttgg caactaaact ccctgaggta
    24961 gaactagtga ttccccctct tcgactttgt ggagataatg ctgcaatgat tggagcagca
    25021 gcacatgtgg aaatgcaaaa aagaaatttt gctagctatc agttgaatgc tgatccaagc
    25081 ttggttttat ctgaataaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    25141 gcccaggaaa aacggcttct catgtttttg acctggctgt tcttattacc gcgtatgcaa
    25201 gcttttgaac gccgtttaat caagagttcg taagcctgtc gtttaactct gattcacaag
    25261 gaacaatttc aaaaaacagc tcctcatgtt tatgaacatt atgacttgtg accgaagagt
    25321 tgacaggtac acaccgttta atcgaaatag ggctgtgaca cgtttttgtc aaagccctat
    25381 tttttacata cttttaatca ttattgttat tttgctattt tttacagctt atttatcatt
    25441 atccgttcct ctgattgccc tcactgagta atcgtttatt gtcgaggaat atcgaataat
    25501 ctagatcgga tgaggacaag acgcttcatc tagttaatct aacagtattt tttgcaaaca
    25561 gtgaatatta cataactgaa acacacatag aaaatctttg agtcttgtcc agtgatctaa
    25621 aactgccgga tctttcagaa gttttaggga ggaaaataag cagtgaccct gatatttatc
    25681 agggtcactg cttatttttg ttcgcgttat tctctcatag ctggagtagt agctgtctta
    25741 cagaaagtaa aacgcttttt gtcataatct aacagcccgg aatcacgtag ttgtttgatt
    25801 acttgtccag cagtttcacg tgtcgtgccg ctattcactg ctaattcttt taaggtgatc
    25861 ggataaggaa tggtgatatg accatctttt gttggtacgc ccaattcttc tttcaaaatg
    25921 aataaatttt gttttacccg gtcaaaggcg ctagaggtca atccgatttg gatacgcaat
    25981 tcatgtattt caactacttt agaaattttt tgtatcatat agatcaactg ttcgggattt
    26041 tttcgtgcta atttttcata gtaggaaaca ggaaggtaga atgtttcaat atcagtcatc
    26101 gcttgagctg tataagtgta ggtctcttct ccaaacattc ctgacatagg aaaaaggtta
    26161 tttgtattca catagtcata gtagtaataa gttgcacttt catcataacg ttctaaacga
    26221 acgagtcctt tggtcaagat atacaaccgt tgtctgggat caccatcgtc gaaaaggatc
    26281 tgtccttttt tataagaacg aagaacagta tgttccttga tctgttggaa ctcttcatcc
    26341 gagaaatgtt caaaatcagg ttgatatctg agttttgaaa agtgaaaatc gattttatta
    26401 acacgcatta aaaagtcctc tccttattgt tcactatatt tattgtcact ttttttaaag
    26461 aatttgtaaa gttttaacat accaacatta gaaacaagtt ttaatacttt acaaatataa
    26521 tttgttatta tgtaaaaaat atacaatggt attgaataat ttgttttgaa gcgaatgatt
    26581 tgtgtataaa aaacaacaat aatacatata gtaaacgcta acaacgataa tataatttat
    26641 ttttttaatt tattcaaaaa aattttttca atgaataaat atttttttga aagtgtatac
    26701 agaataaaaa atgaataaat tacgcttaaa aatagaaaaa cgtttaatta tttaattatc
    26761 gaatattaat gtgaggaatg tctcattttg aatgagacaa aattatgaaa gtgattacaa
    26821 aaggaggata tcctatacat gtaatcaaaa agaaggaggc aacacaacat ggataaacct
    26881 attcacgttt tctctgaaat agggaaatta aagacagttt tactgaaacg gcctggacaa
    26941 gaagtggaaa atttgacacc agatattatg gatcgtctgt tgttcgatga tattccatat
    27001 cttccgattg ctcaagaaga acatgacaac tttgcgaaga cattgcaaaa tgaaggcgtc
    27061 gagactcttt acttggaaaa attagcggca gaagcgattg acgcaggcaa tgtcaaagaa
    27121 caattcttga ataaaatgtt ggatgaatct catattgcat cgaatgctgt aagagagggt
    27181 cttcatgagt tccttctatc aatggaaacg caagaaatgg tagataaaat catggctggg
    27241 gttcgtacaa aagatatcga agtacgttct agcagtctat atgatctgtc agcagacgat
    27301 gactatccat tttacatgga cccaatgcca aacttgtact ttacacgcga cccttccgca
    27361 tcaatgggaa atgggatgac agtcaataag atgacttttg aagcacgtcg tcgtgaatcg
    27421 atgtttacag aatatatttt gaaacatcat ccaagatttg caaataaagg aatcgaagta
    27481 tggttggatc gtgaaaatcc agatcatatc gaaggcggag acgaattgat cttaagcgat
    27541 aaagtagtag ctgttgggat ttctcaacga actaatgcga aagctttaga aaaattagca
    27601 cgccacctat tcgcaaaaaa ttcaggattt gaaaaagtgt tggcaatcaa aattccgaac
    27661 aaccgtgcaa tgatgcattt ggataccgta tttacaatgg tagatcacga taaattcact
    27721 atccatccag caattcaaag taaagacggt aagatggatg tctttacaat cgaaccagat
    27781 ggtgatgata tcaagattac tcattcagat aacttacacg aaacgatgaa agctgctctt
    27841 ggattagatg atcttgtact gatcccaact ggtaatggag atgagatcgt tgcacctcga
    27901 gaacaatgga atgatggatc aaacacattg gcaatcgctc caggtgttgt agtcacttat
    27961 aaccgaaatt atgtatcgaa cgaattgctt cgtagctatg gcatcaaagt gttagaaatc
    28021 aattcaagtg aattgtcacg aggtcgtggt ggtccacgct gcatgagcca accacttgtc
    28081 cgtgaagact tgaagtaatt tgtttcctga cggcagtctt actgtcagct gccgtcggga
    28141 tttcctcttt aatcttaaat aaaaacaatc taaaggagaa tgtttaatat gaaagaatca
    28201 gtattccaag gaagaagctt attagcagaa aaagacttta caaaagaaga attacaatat
    28261 ttgatcgatt tttcagaaca cttgaaagac ttgaaaaaac gtggtatccc acatcactat
    28321 ctagaaggaa aaaatattgc gcttttattt gaaaaaacat ctacacgtac tcgttctgca
    28381 tttactactg cagcgatcga tctaggtgca catcctgaat atctaggtgc aaatgatatc
    28441 caattaggta aaaaagaatc cacagaagat acagctaaag tactaggacg tatgtttgac
    28501 ggaatcgaat tccgtggatt cagtcaaaaa atggtagagg aactagcaga attctctggt
    28561 gttccagttt ggaatggctt gactgatgaa tggcatccaa cacaaatgat cgctgacttt
    28621 ttaactatcc aagaaaactt tggaacagta gaaggtatca ctgttgctta ctgcggagat
    28681 ggtcgaaaca atatggctaa ctctttatta gtcacaggtg caattttagg agcgaatatg
    28741 cgtatcgttg caccaaaaga attacaacct gaagaagaaa tcgtcaaaat ggcagaaggc
    28801 tttgctgaaa aatccggtgc acaattgatg attaccgacg atgttgataa aggtgtagac
    28861 ggagcagacg tcctttattc tgatgtatgg gtatcaatgg gtgaagaaga taaattcgaa
    28921 gaacgtatca aattgttgaa accttaccaa atcaatatgg aaatggtaga aaaaacacac
    28981 aacacagatc gtttgatctt cttgcactgc ttgccagctt tccatgatac aaacactgtt
    29041 tacggtgaac aaatgaaaga acgctttggc atcactgaaa tggaagtaac agatgaagtc
    29101 ttccgcagca aatatgctcg tcaatttgac caagcagaaa atcgtatgca ctcaatcaaa
    29161 gcgatcatgg cagcaacatt aggtaacttg tttatccctc gcgtttaagc ataaccacat
    29221 tcatctcacg aaaaaacgag tcagcattga gggaaaatca caaaacgctg gctcgttttg
    29281 ttatctagaa aattcggtag aactgaagaa aggacgaatg aaatggccaa acgaaaagta
    29341 gttgtcgctt tagggggcaa tgccatcttg tctacggatg caagtgcaaa agcccaacaa
    29401 gaagcgttaa tggaaacagc aaagtattta gtgaaattta tcgaacaagg agatgaattg
    29461 atcatctccc acggaaatgg tccgcaagtc ggtaacctat tgattcagca gcaagctgct
    29521 gattctgaga aaacaccggc aatgccttta gatacatgtg ttgcaatgac agaaggatct
    29581 atcggttact ggttacaaaa cgccatgggc gaagtactga aggaaaaagg gatcgataaa
    29641 gatgtggttt ctttagtcac tcaagttatt gtggatgaaa atgatccttc attcaaaaat
    29701 ccaagtaaac cagtcggtcc attctacacg gaagaagaag ccaaagaaca aatgaatgct
    29761 gactccactg tgacattcaa agaagatgct ggacgtggat ggagaaaagt agtcgcttcc
    29821 cctaaaccca tctcgattaa agaagcacgt gtcatcgaaa cgctggtcga tcaaggagtt
    29881 atcacagtct ctgttggtgg cggtgggatt cctgttgtag aaacagctac tggattagaa
    29941 ggacgagaag ccgtaatcga taaagatttt gcttcagaaa aattagccga aatcatcgat
    30001 gcggatttgc tgatcgtatt gacaggagta gacaatgtgt atgtcaatta ccaaaaacca
    30061 gatcaaaaga aattagaaac tgttactgtt tcagaaatga aacaatacat tgatgaaaag
    30121 caattcgctc caggaagcat gcttccgaaa gtagaagcag ctattgcatt tgttgaagca
    30181 aaaccaaacg ctaaggcaat catcacttct ttagaaaata tcgagaatct tttagcttct
    30241 gaagaaggaa cgattatcgt agctgattaa tttgttaagt ctttcgtatt tacgaaatga
    30301 tttatagcta tccaggtggt atttcttctc ctttaggcca tctggatttt t
//
LOCUS       DACCCS010000033        30276 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.033, whole genome shotgun sequence.
ACCESSION   DACCCS010000033 DACCCS010000000
VERSION     DACCCS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.033"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..230)
                     /locus_tag="IVK82_001530"
     CDS             complement(<1..230)
                     /locus_tag="IVK82_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP6392598.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKI"
     gene            complement(243..3002)
                     /locus_tag="IVK82_001531"
     CDS             complement(243..3002)
                     /locus_tag="IVK82_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP6392599.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            complement(2999..3703)
                     /locus_tag="IVK82_001532"
     CDS             complement(2999..3703)
                     /locus_tag="IVK82_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6392600.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(3715..6024)
                     /locus_tag="IVK82_001533"
     CDS             complement(3715..6024)
                     /locus_tag="IVK82_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP6392601.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(6219..6683)
                     /locus_tag="IVK82_001534"
     CDS             complement(6219..6683)
                     /locus_tag="IVK82_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392602.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(6683..7309)
                     /locus_tag="IVK82_001535"
     CDS             complement(6683..7309)
                     /locus_tag="IVK82_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6392603.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(7316..7681)
                     /locus_tag="IVK82_001536"
     CDS             complement(7316..7681)
                     /locus_tag="IVK82_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP6392604.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(7671..8009)
                     /locus_tag="IVK82_001537"
     CDS             complement(7671..8009)
                     /locus_tag="IVK82_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392605.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(7999..8325)
                     /locus_tag="IVK82_001538"
     CDS             complement(7999..8325)
                     /locus_tag="IVK82_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP6392606.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(8303..8584)
                     /locus_tag="IVK82_001539"
     CDS             complement(8303..8584)
                     /locus_tag="IVK82_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP6392607.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(8586..9950)
                     /locus_tag="IVK82_001540"
     CDS             complement(8586..9950)
                     /locus_tag="IVK82_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP6392608.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(9963..10538)
                     /locus_tag="IVK82_001541"
     CDS             complement(9963..10538)
                     /locus_tag="IVK82_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAP6392609.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(10504..11691)
                     /locus_tag="IVK82_001542"
     CDS             complement(10504..11691)
                     /locus_tag="IVK82_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP6392610.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(11755..13482)
                     /locus_tag="IVK82_001543"
     CDS             complement(11755..13482)
                     /locus_tag="IVK82_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP6392611.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(13479..13916)
                     /locus_tag="IVK82_001544"
     CDS             complement(13479..13916)
                     /locus_tag="IVK82_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAP6392612.1"
                     /translation="MTTKAQRKAIIDEKARILEIMNLSDLYTITLDPLIESYLDIFEI
                     YQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGLTNKAK
                     TGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(14028..14408)
                     /locus_tag="IVK82_001545"
     CDS             complement(14028..14408)
                     /locus_tag="IVK82_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP6392613.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(14405..14791)
                     /locus_tag="IVK82_001546"
     CDS             complement(14405..14791)
                     /locus_tag="IVK82_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392614.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(14793..15071)
                     /locus_tag="IVK82_001547"
     CDS             complement(14793..15071)
                     /locus_tag="IVK82_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392615.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            15119..15847
                     /locus_tag="IVK82_001548"
     CDS             15119..15847
                     /locus_tag="IVK82_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAP6392616.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(15993..16469)
                     /locus_tag="IVK82_001549"
     CDS             complement(15993..16469)
                     /locus_tag="IVK82_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAP6392617.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(16429..16776)
                     /locus_tag="IVK82_001550"
     CDS             complement(16429..16776)
                     /locus_tag="IVK82_001550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392618.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(16763..16948)
                     /locus_tag="IVK82_001551"
     CDS             complement(16763..16948)
                     /locus_tag="IVK82_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392619.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(16949..17221)
                     /locus_tag="IVK82_001552"
     CDS             complement(16949..17221)
                     /locus_tag="IVK82_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAP6392620.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(17218..17421)
                     /locus_tag="IVK82_001553"
     CDS             complement(17218..17421)
                     /locus_tag="IVK82_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392621.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            17546..17758
                     /locus_tag="IVK82_001554"
     CDS             17546..17758
                     /locus_tag="IVK82_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392622.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(17791..17985)
                     /locus_tag="IVK82_001555"
     CDS             complement(17791..17985)
                     /locus_tag="IVK82_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392623.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(17993..18181)
                     /locus_tag="IVK82_001556"
     CDS             complement(17993..18181)
                     /locus_tag="IVK82_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392624.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            complement(18808..19173)
                     /locus_tag="IVK82_001557"
     CDS             complement(18808..19173)
                     /locus_tag="IVK82_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392625.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(19213..19515)
                     /locus_tag="IVK82_001558"
     CDS             complement(19213..19515)
                     /locus_tag="IVK82_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392626.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(19515..19814)
                     /locus_tag="IVK82_001559"
     CDS             complement(19515..19814)
                     /locus_tag="IVK82_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392627.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(19977..20246)
                     /locus_tag="IVK82_001560"
     CDS             complement(19977..20246)
                     /locus_tag="IVK82_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392628.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(20258..21007)
                     /locus_tag="IVK82_001561"
     CDS             complement(20258..21007)
                     /locus_tag="IVK82_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6392629.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(21010..21696)
                     /locus_tag="IVK82_001562"
     CDS             complement(21010..21696)
                     /locus_tag="IVK82_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392630.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(21702..22373)
                     /locus_tag="IVK82_001563"
     CDS             complement(21702..22373)
                     /locus_tag="IVK82_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP6392631.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(22366..22707)
                     /locus_tag="IVK82_001564"
     CDS             complement(22366..22707)
                     /locus_tag="IVK82_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392632.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(22885..23583)
                     /locus_tag="IVK82_001565"
     CDS             complement(22885..23583)
                     /locus_tag="IVK82_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP6392633.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            23670..24140
                     /locus_tag="IVK82_001566"
     CDS             23670..24140
                     /locus_tag="IVK82_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392634.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(24183..24362)
                     /locus_tag="IVK82_001567"
     CDS             complement(24183..24362)
                     /locus_tag="IVK82_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392635.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(24349..24687)
                     /locus_tag="IVK82_001568"
     CDS             complement(24349..24687)
                     /locus_tag="IVK82_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP6392636.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(24703..24906)
                     /locus_tag="IVK82_001569"
     CDS             complement(24703..24906)
                     /locus_tag="IVK82_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6392637.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(24921..25697)
                     /locus_tag="IVK82_001570"
     CDS             complement(24921..25697)
                     /locus_tag="IVK82_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP6392638.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            25996..26316
                     /locus_tag="IVK82_001571"
     CDS             25996..26316
                     /locus_tag="IVK82_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6392639.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            26334..26765
                     /locus_tag="IVK82_001572"
     CDS             26334..26765
                     /locus_tag="IVK82_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP6392640.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            26895..27356
                     /locus_tag="IVK82_001573"
     CDS             26895..27356
                     /locus_tag="IVK82_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP6392641.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            27390..28025
                     /locus_tag="IVK82_001574"
     CDS             27390..28025
                     /locus_tag="IVK82_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP6392642.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            28138..28773
                     /locus_tag="IVK82_001575"
     CDS             28138..28773
                     /locus_tag="IVK82_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392643.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            29072..30211
                     /locus_tag="IVK82_001576"
     CDS             29072..30211
                     /locus_tag="IVK82_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6392644.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT         8920 a         6079 c         4732 g        10545 t
ORIGIN      
        1 ttgattttcc cttttttagc atccacaatt cttgtttcta attggactgg atattttccg
       61 ccaacaactg attcaaaata taccttacat tctgataaat catatggaag accattttcg
      121 acaattgtag cttccataac ttcagtgttt ttattgcctt gtcgaacttg aatcatcccc
      181 acgtaattat agggttctgt tgtacttaat atgacattcc actttgccat taaatcacct
      241 ccctattttg gtggtatgac aatggaagaa attgcacttg cactataata ctggcgatct
      301 aactttccac aaatcatacc taactctgtg ttttgttcat aagtttgcat acgaccatta
      361 gctaagcctc ggataacgcc agtatgtcca taagttccgt ctgcaaacca tgaacccact
      421 tgtccacctc ttgcccaatt aataattgca ccaactacta attgatcata tctagggttt
      481 tgaatcactt tccaaccaac agcagaccaa tcataggcaa taccaatatc agatgctgca
      541 gaagtatttc ctatcacatg agttaaacca tattttgttc cagcacccat gccacaacca
      601 cccagatatc ctgaatattc tgcagacaag ccataacact gaccattgcc aattcgttgt
      661 ccaatcaaag actctaaatg ttttagtcct gcttctccag tagctcctcc tggttttaaa
      721 tctttaaatt tgttatacca gttaactgca taatcttgac gttcaggatg tgtcgctgcc
      781 ggacgttcat agtttcgttc aaaagcatat gctgcttgtc ttggatcagt acaggcctta
      841 aatccatcaa ccgtcgtagg ttgtactacg cccatccatt gtccatttgt aaatgtccaa
      901 ataagcaatc gaacttgtgc atctaagctc gttattggtt ctttaatacc tgcagcatta
      961 aataagtttt gaacataaac ttttccatcc catgttgctg gaccaacaag aggatatgaa
     1021 gaaccatccc actgaactaa tccgtatgct ggtccaccta tttgaacagt atcaggatca
     1081 aaagttcctc ctgtttcttg ttgaatgttc cccaatattc cacatgcaga ttgtttcgta
     1141 aatccgttat tacacaaaat atcgtatatt ctccaagctc tcttttctgc atctgttttt
     1201 aattcatcag gatacccacc aggtgtttct cctccgccag atggaccgcc attttgtcct
     1261 ggtatcactt ctttacctct aacagtaagt tttccctgaa tatctaaatc accgaaataa
     1321 tatgcttttc cgtttcctaa taacacaaat cctttttctg cagttggaga aattaaaatg
     1381 tattttccat cgccatttgt acgtattacc aaagaatttt cctcaagagg tgtaggtgtt
     1441 acagcatttg gaaatggatt tcctgctgaa tctgttgttc cgatcgttcc aatggattct
     1501 tttctattcc agaattccat accttttttg gttagctcca tgattttttt gttttcattc
     1561 caaatttgaa gcgtgccttt tacaagtttt aacacatcac cataagcatt aaaggaagtt
     1621 tcgaatactt ctgcatttat tgttcctact ttgatgaagt cagcaacaat ttctcctttt
     1681 gatgtgatag caataccgaa cgggccattt actccgttgt ctgaataacc taagccattt
     1741 aaattccaac gccacactcg ctttgcattc gccacatttg gagtatccat gataagaatc
     1801 tctgatggag ctttttctgg acgaaaaacg acatgcccac cagaattacc agtgatccat
     1861 gccgtcgcat tcaacacatt ttgaactaac gtttctgttc gattgtcgat tttctttttc
     1921 aattcttgtg cttgagtatt tactgccgaa gtgtacagtg ataaatcatt acctaaaaca
     1981 atatccttat atttcccaag cgttgggaaa taagtgtatt cgaccatacg ctcttttatc
     2041 tcgatatcta gctctttcgc cctaacatga gcgacatcac caaagtgtaa agacgctagc
     2101 tcttgataca tgtcaccgta ttcgagtgtg tgttccaacg ccaccatact gactgtatga
     2161 gtagcttttg gctcatggat acgatcattg tcaaataagg ttttgcccca tttgatcaat
     2221 tcatcaactg ttttacaatc tccgttttca cgtttggcaa tacgtcgatt ctcgttggtt
     2281 actccatcaa tttctaaata accatattcg ataggttctt tgtcttcatc ataatcgtta
     2341 tcaggaacac cgccaaccaa atacaaacta ttgacaattg attcttcatc aatttcttct
     2401 tcgattgctt ctaaattgat gccaaaatca atccgaaaac cattatctga tccaatctgt
     2461 tttaccagtt tcaaatcata gttatccata tcaagttccg cgctagtcac gccagtcaaa
     2521 ttttgattac cgttatttga accaatgatt gcttcaactg gcgcgacttg tcttgctgta
     2581 aattggtgcg ttgtccctac attcgaaaga tagttaaatt tttgatcaaa tgccaaactg
     2641 tttttaagat tggtcatgat ctgagaccca ttgccgttat ccgtaaacga cttgacaata
     2701 aagttcttat tggccataaa accaatatgc cgagctgtta cagaaacgga ggtcaaattc
     2761 tttttgacat tataaatttc aaaatattgc catgatccat ctggtacctt cgcttttata
     2821 aaatttcctt tttttaaata agagcgatac tgtccgcttc ttgaatagtt accataaaaa
     2881 cgatattggc cattcagcac acgattgatt tctggcaaat cttcccaatc aactaacgac
     2941 gctccgttca cgctcaaatc atctggcatt tttttataag cataaataaa ttcttgcgtc
     3001 acagatacac actcctattc caaaaccgca cttctttaaa ttttccggat atttttacct
     3061 gattccattc aggctgcaat actggccaat caccacgcgt aaataaattc aaaccttctt
     3121 gtattgcttt tccaagctga gtatcaacca cgattgttgc agctaacgta ttaagtattg
     3181 ttaggctttt atcaccgaca gcgatcgtta tatctcctcc gttcgattct atttccagat
     3241 agggatgtgc aatttcgtca ccatgatcaa aaatatccat aacactagaa tgaaaaattt
     3301 taggtgcttc accgattttt cttttgagtg gctgacaacg aaaagtaaca tcgaatgtat
     3361 aaaagaagcc ccactcgttt tcgaaaggga cttctttatc catgctgcag atagcctcta
     3421 gatacttgtc tggatcatta tgtgtgatta attggctttt accagtgagc catcgtttga
     3481 cttctctcag tttcgaatgt ggaatagtga tcccttctat ttccaaatca aaaggttcat
     3541 agtcatcaaa cgtctccgtc aattctccgc ttcggccctg aatcgtgtaa gtttcgtatc
     3601 gcttgttcgg cataatatct ggaagctctg cctcaatgat gcaattcata tcaattactg
     3661 cattccgatt tttccaaata aaatttggtt catctggatt cataaattgt cgactcaaat
     3721 aggaacacct cccaaatcac gaatagcctg tttgtttgct ttagcaaatt tacgattcat
     3781 acgatctaac tcagatggat tgtttgcatc gactttgcca atgtgtatat gctgttcaat
     3841 gctacctcca gaaactcttc cactgatccc tttgcgtttt tcttcgtctg ataatggagt
     3901 aactgtggtt ttaccattct tggcagtcag taactcaggt ccagcttcac caacaatcgc
     3961 ttgtccattg attagatgac cgccttgtgc taaataaggc agttttgaaa tagaaaatga
     4021 ttttccacct acaccaggaa cccattttgg tatctttata ttattcaatc caccaataaa
     4081 tccattgatc aatccaatca tggcattaat aggagctttc cctacagcaa caataccatc
     4141 gaaaatacct ccaaaaatat caactagacc ttgccatgct tttgaccaat tccctgtaaa
     4201 tactccagtg ataaaatcta gaaaaccact gaaaattcgt tttccagcat tataaaaatt
     4261 attgaaattt gctataacac catcgaaaaa tccaccaaaa taaccactta aaaaattaaa
     4321 tatttcaaca gctacatctg aaacaccttt gaagaaagaa ttcactccat catgaaacca
     4381 tttaacgtta ttatatgccc aaattagtcc agtaactaat gcgccaatag ctataactgc
     4441 taatgcaaac cagcctcccg acattccaaa taaaccagca agtccttgcc aaactcctat
     4501 aaaactttta acttccccaa cgatttttgt aacagatccc ataagtgaac caagaacgat
     4561 tagtactggt ccaactgctg ccgctatacc agcaattgtt atgatccaat tctttgtatc
     4621 ttgatctaga gacccccacc atttagagaa ttcctgtaat aaggaagttg ctttttcaaa
     4681 aaaaggcaaa aggcttactt ggactgcttc acctacgtca gccattgcta attttgcatt
     4741 gttcatagct tgatcggctt gatcaattgg gtcgagagta gcatcgaatg tgtctcctac
     4801 agctccccca ctttctccag cagtttttgc taaatcttcc aggttcaaag tacctctacg
     4861 aattgcatcg gccattctag gaccgccttt agtcccgaat acttctgctg ctgcattgat
     4921 ggcttctgtt tcagaacctg cattttttac cttgtcttgt agttcttcta gtccttgact
     4981 taaagatttt ccgtctttcg cataagccac tgttgcttta gataaactac ttaaagctgc
     5041 gcttgaatca acaccagatt gctcaaattg tccaagtaaa gtgacccctt caccaaagct
     5101 caaccctaat tgtttaattt gtggtgcgcc atcaattgct ttttgcatca agtcgtctac
     5161 agattgacca gtattctgcg atgttttcgt tgttacatca aggacagaat tcaaatcatc
     5221 atattctagt ccataagcat caatagcttg tcgagcagat attgctgatt gcgaaacatc
     5281 tgtatcattg atttcagcgt actgtaggag ataattggtt gaatcttcca gttttttatc
     5341 catgaatcca aattgtgtat ttacctcacc aatagcttcc ccaaccgttt gtaaaggtaa
     5401 atgagtattt gaaccaacgt ttttgaaaga ctgtgaaagt ctatcagctt gatcacctgt
     5461 tgctccggtt tttgtaatga tggtatcaag tgcctcgtca acttcaccaa atgctgcaag
     5521 tcctgctgct cctgccgcta caataggcgc ggttactcca acagacattt tttcaccaac
     5581 gccttttact ttctcacctg tttcttcgat tttctggagt ttttttgctg tatcaacaga
     5641 aacgtcacct tgttctttaa gtgcatcgtt cgtctgttct aatgctgtac gaagtttatt
     5701 ttctccggtt tcagaattga gtaattgttt ataaagtttt tctgattcag cagaatattc
     5761 accggtttct ttgacagatt tttcatattc ctctctcagc atttgagatc gttgttcagc
     5821 caatcctaat tgtttttcta atttttgttt agccgctcgc aatttttctg tttgagttgc
     5881 atccttgtcc atcgcagaca cttggttttt gtactcggca gcggctaagt tcatttcttt
     5941 gttgatttct ttgattgttt tcgaataact gacttcgcca ttcgttttaa aattaagaac
     6001 gacatcagat tctttaccag ccatttatct tagcgctcct ttctaccacc aaggcgattt
     6061 atccatagtt acagattgag gtggttcaaa atcggtgttt tgttgtaacc actgtaaata
     6121 agatttgagc cacaagttag gtgtagactt taaaaagaaa ctctcactcc aattcaatag
     6181 agtgagagca acgtaaatat aaaaactcca aggagttcct atctcttccg attctttttg
     6241 tttttctttc tttttgcttg cgctttttga aagtcttgtg gcttcttgga tttttttagg
     6301 tcttccacct gaaatgtctg gctagcaaaa atttccatac aggtactata agcagacaac
     6361 acctctccac tcattcccaa aaatttgaaa atagtttctg gatcttcctc taaaccacca
     6421 gtacgcagca tggcataaat caaggcacgc atgattttta gatcactagg cgataagtta
     6481 gcagaagaaa tttgtccttc ttttttagat agcatggcgt tcatatcaga ttcaaattgc
     6541 gaataatctc caccatacac atctgctata aattccatcg tttgcatagt aaatgaaata
     6601 gggaattctg cgccttgaat aattacaaca gcagaatttt ttaagtcttc aacgtgaatt
     6661 ccataatcag ataaccgagc cattaacccg cacctccaac tttagccaat gttttccact
     6721 gttcttcatc gtatacaggt tgagcaatga atttattaaa taattctaga gaagctccgt
     6781 ctctatttga atcaaaactt gcatacatca cattattgta tttcagtccg atggaaacca
     6841 gatttgcagt tacatcgtca atttttgttt cttcctctgc agtggcatac tcttcgtcaa
     6901 tcacattgga tagttgtgta ttaggatacc aaactgcttt tttaccgcca ttttcaatat
     6961 ttccaataaa tccaaaggcg aagtacggaa attctcgtgc ggtatttttg ctaaacgtta
     7021 ctccgctttt agcaactaat cctttgattt catccatcac ttctatcgga atacccacgt
     7081 gatccaacgc aagttcatgt tttgtttctc gcgacacacg acgaaacatt ttgctggatg
     7141 cccatttttc taatgctgtt ccattccctt tgacaccgat ttttgtggca attggcaatc
     7201 gtaaaacttc actataagtc ggtgcagttc caacagaatc tggcgttgcc atcatcgcaa
     7261 ttaaaatgtc gtccaatcct tcaaaataaa aagtatcttg ttttcccaat taaaatcacc
     7321 cttcccataa atcaagtatt tttctagtca taatttcttc aattttttct ttgttttgtt
     7381 catacgtacc acttgcaaaa tgttgggcac gttgatttgt tgtgccattt tcggcaaagc
     7441 gccagtaaaa ggccgtctct tcaaattgaa ctttcacttg atcgttttca actactactt
     7501 ttatttgatc gcgcatatgt ttctttttta agagcgactt aggaatatta ggaagcaact
     7561 gctttagata aaagttagct gcttcttcta aagattccaa cgataatttt ttcggatcaa
     7621 cttttgaaag gtttcctaaa taatccgata tttcagaaaa accattctta ttacttggca
     7681 ttttccacac acctcacata tgtgtaatag ttggttacgg tatcgtcgtt ctcatcacct
     7741 tgtatacctg taaaatctga atatggaatg ccagcatttt gcagcgcttg ttcaataaca
     7801 actaaatcct gttctgttcc aagtgtaaaa aaagagactt ggtaataagg taatttttta
     7861 tagactttac cggatgccat ttttttgctg ttactcacat ttgagtacac aatgtatgga
     7921 tacatcgtcc ctaatctggc tttgtctctg aacactgcta actttgttga tttcagcgct
     7981 gttttcaatt catcaaagct aatcgacata agctaaactc aactccattt ctcttgcatc
     8041 gggattcgta taaatgcgag taatgttata cgttacagaa tcaattttga gcgcacttaa
     8101 tttctctgtg atggatttat cccatctgac tttaattcgt ctgacaacgt ctgtcttggc
     8161 ttgctgtgat aaatattttt cttgagaagt cacaccgagt tcttcataga atattagacg
     8221 cttaaattcg taaattgtag ttggacgatc gtttccgtct gttcctgttt tgatgtctag
     8281 caattcggct ttccatctga gattattggt ctgtctcttc ggcattttga atcgctcctt
     8341 gcacgataaa tggcgtcatg gcattcatag ctttgtcgag ttcatcctct gaaactctat
     8401 attcataggc aatgccggca accatcaaaa taagatattc ttgttggcca ccagttgctg
     8461 ttttgacata atcttttgcc atatttaaat aaaaagagag caaagaatca tccatgccct
     8521 cttcaaaatg aatatgtgat ttgaattttt cctctaaaga caattcttta gtttgctctt
     8581 ccatcttaac caccaactgg ttttgtaatt tcgtagcgat atactgccgg ttcaaatgga
     8641 gaataaacca attgaccatc tagcaagttg taaatttgga atccaatttg atttttacca
     8701 gagaattttt caacaagttt ttgaatttcc aaggcaccaa taacttcttg aattttaaat
     8761 gcagaaaaat cgccaaaata taaaactggc gtgtctggtt cacccttttt atctgctgca
     8821 tctgtccaat ccacaggata gccaactaat tggtaaccaa ttccaccttc tgcttgtgtg
     8881 aatggccgca acaaaggaaa tccatcatct gttttcattt tttcaatggc agtcaaagca
     8941 gctcgattaa taataaagcg tccctttttc atcacttctg tcactggtgt atttttaaat
     9001 tcgattaatg catcatataa tttttgccca gcacctgaag cagttagatc taaaggtttt
     9061 ttaaatgcta cagccttttt ggctaatgca ccaggatttt catttccagc gtcatcacca
     9121 ttgaacatat aattgatttc tttacgcaca taagcttttt tcaattcttc cacaacaata
     9181 tcttcaactg gaacaccaga catttttaat aattttttag ttactgttgc caaagcatcg
     9241 aattcggcag gattaagcaa aatttcatca aactgaatag ctgtttcagc aatatcagtt
     9301 gaacgctctt tcttgtttac attcgcatct gctttcttca caagaattgg atatttgaca
     9361 tctcctgatg ttcgcaccac tgttccgtat ttacgaagta aattttcttc ttgagcataa
     9421 gtaataactt cagatgcaat tacttctggg acagtaactg aaccgttgcc agcttcaatc
     9481 cctaaagctc gagcttctgc ttcagaaata tttccagcta caaaattagc aaatgctttt
     9541 cgtagttgtt gatctttttg tttcttggtc atttttgcac gtgcctccaa tccgttctta
     9601 attgatccaa gtaatccatc tcgttgctgt tgagtaatca ttccagaacg attttctgaa
     9661 ctatcttcac tgtctgaatc ttcttcttga tcgttgtctt caccagaacg gctttcgtct
     9721 gaatccgtac tattcgattg atcatctgta ttgtcgtctg tttcatcagt tccagaatct
     9781 gcgcctaatt cgtctttaat tccgttcaat tcatcaataa cactgtcaat ttcttctttc
     9841 acggcttcta aatctgcttc gcgtaattct cctgattcaa ctttttcacg taattcagtc
     9901 aatcgttgct cacgacgtgc cttcattttt ttcaataatt ctttatccat gtatttttcc
     9961 tcctacgctt ctagcgcttg attaattttt ttgattaatt tttttctttc ttcgacgttt
    10021 ttttctaatt catcacgact tcttaatgca gcttctgtat cttcgtatgc tggcaaaggc
    10081 acaatagaaa cctcgtataa ttccacttca tggattgttc gaagcattgg ttcagaattg
    10141 tagtcccacg tttcttcagt cggcacaaaa ccaaaactac attggttgat gtctcctcgt
    10201 tccatagatt taactaagtc ccttgctacc gttgtgtccg gtaaatcaac ttcgaatttc
    10261 aaaccacgtt catcttcttc aaggcgaagg gttccgcttt tagtacgtcc tagcacattg
    10321 gaccaatcgt gattgaacaa acaacgaaca tccgaattac taattgcctt tgagaatgca
    10381 ccaggaacaa tcacttcgct taagtcatcc cataaaagtg tttgactgtt gaatacagca
    10441 gcgtagccgc taatggtcct cgtatgagtt tcttcatcgg aacgtgttga aagattggtg
    10501 atgtcaatcg tgcgaatttc cttcttcttc atttccatca cctcctttca agtcttgatc
    10561 attcgttggt aaggaatcat ctgttgcatt tttctggcca atcctagata agtcatttga
    10621 aatatagata gcttgtgttt ctggagtatt ctgtttagga aaaccaagca tttctgccac
    10681 gttatctgga cttgtaatcc cggtacgaac gatgttgtag ccaatatttg tttttgttga
    10741 gtaaggaaca aaatccaaaa tattaatttt ccattccact ctatagccag aattaggcat
    10801 aaaaaaaaga gcggtgtaat gttcgctctt gtttttcaat attggtttga ttgctttgtt
    10861 gtgcagatac atcatcgctt tttcaatatc tgtcttcatc agcgattgat acgtatcaac
    10921 attgattcct agaaattttc ctaagtcttt tttgtaaaca cctaaataat tcaaaattgc
    10981 cgcgtcatca acaggacttt ttaatgtctc gatggaatat ccttttccca aaggaatcat
    11041 cttaacagaa tgatcactct cattttgcgt tccttccagt tgatccaata tagctttgac
    11101 aatttttgtt tgggcgctat tatttggatt gatgtgggcg tctagtttaa gcatgaacgc
    11161 aagtaaaccg cctttagtat atttgtccgt caaaactttt tcagcgctca gaacgccttc
    11221 tagagtgttt cttgcaagat caattattcc agcacctttt aatgaatcag ttccgatgtt
    11281 cttaatgtgt cgaatcattt gaccaggtat tttttgacca ttcatttcaa attcttcttg
    11341 aagtcgttca ttgattttag ttgttacacc gtacgccaaa tgaagctgat ccctatccgt
    11401 taatgggaat gtctcaccat tgatcaataa agtatttgtt tccaatttgg taaattcgaa
    11461 tcctgtcaaa taatcattgg gactcttcaa aatatttagt aagtgatgat ctttgacttc
    11521 gttgccgtcg ggaccaataa caattggtgt ggccaatgcc acctgattag atatgtcttg
    11581 aactaattca taaacatcag aagattccat gatagatgaa tccgtcacat atctttgacc
    11641 ataacgtgtg tagtgtccaa aaatatcctc gatatatcca cgtttttcca taaaagaata
    11701 gactgcattc gataaccgat cacgtaattt caattttttt caccgccttt ctatttatct
    11761 atagatggaa cttaggtaat catctaactc atctgaatca atatctgtca tttgattcat
    11821 cgtttcctta tgaccacaca aaaacgccac gaacccatca atctttttct ttgattgacg
    11881 tttacttggc gctttttgtc cattgatgtt agtaattgct acaacgttca aagtgcaata
    11941 aaggaataat ggattatcaa attgaattcg tttctcataa aacaaccgtt cgacatcgtc
    12001 aaaaggagca ttcaatactt taggatattg agcaacttca acgcattcca atcctaagtt
    12061 ttctaatttc tcaacaagtt tgtcactcat cgctggatca taattcactt gctgaatatc
    12121 atataaattc atgcagtcct ctatgtactg caaaatctga tcttgatcaa tcatttttcc
    12181 gtcgcaaaat tcaacgaaac cttgttcagc caaatcactg taaggaacat tatcttcttt
    12241 ttctcgaaac tctaaatctt cattcggaat aaaataaagt tgctttactt taagaaccgc
    12301 tttaccatct tcatcccatg ttgggaagtt taaagataca caggtcaaat ctcgtgtacg
    12361 tgataaatct aaaccgatat aacatggttc gccacttaaa tcaccaagtt cttgtgtggt
    12421 aactaaacat ggctctactt gatcctgttc aaagaaatta tccgcgccgt tcacaaatac
    12481 atccaagtgt ttcgttagaa attcagcttt ggaatgagct gaccgttgcg ctgttttaaa
    12541 tgccgattct aaagcagata aatcgactga tattccccag ttaggattgc acatttccca
    12601 gacttttcta tccgtccaat cataattttt atttggctca tagattagaa caaagtttga
    12661 atcattatca tcacgtttca acacttcttt tgcttcgcga tatacacgca ttccaacaga
    12721 cgacgaacct ttcccagcag ttgagatatt aaacatcaat ggctgtggta aagatatctg
    12781 agcagactta aaattgtcat actgttccat tttctcctgt ttatgcaatt catcgttcaa
    12841 tacaaaatat ggattggatc cctcgatatt atcaatattc ttggtttgaa caataaattt
    12901 atttgagtaa gccatatctt catgaatata gtcataagta atactagaaa cagttccctt
    12961 tggcccccta aatattttag ttccttcaag taggattgga ttgtttaaga ttgtaactgc
    13021 aaaaggtttg gcagcatatt gcgcttgagc aaaatcagat gcgcaagcat agcaatcaac
    13081 tgataacgcc ccttctccgt acattgcata tcctaatgaa ccgacagcaa tcaatgtttt
    13141 accattcttt tttggaatct ggacataagc ctcacgagta acgcgtacaa tttggccttt
    13201 ttcattttct ttaacccaac cataaatcca agaataaata aatttttccc agggttctaa
    13261 aagaaacggt tttcccacca tttctccttt tgtgtgaaca ataaatgatt ctacccaatc
    13321 catcatttca ttcgcgcgat ctacatcaaa ccaaatatct tttctttttt tccactgata
    13381 ccagcgatct attgctaaac gaacggtctt aggatacttc ttaggtttct ttcttacttc
    13441 ttttgcaaat aaatcagcat aatttacacc aggttcaatc atttttcagc acctgccttc
    13501 ttacgccatt tatttctgtg cgctgctagt tcatctactg gctttacttc tgggcgtgta
    13561 atttcttcat cttttcttgc ggtcgatcct ccagcaatta gtctaccagt ttttgctttg
    13621 tttgttaatc ctaataaatc tagagctttc atcttcttat ctgcccaagt ttctacttgt
    13681 tgtgccaatg gatgttttga ttgattagta gctcctgatt tattcgtgaa tttttgcgtc
    13741 tccggaaatc ctttttcctt ccacaataaa tatttgtgtt ggtaaatttc aaaaatatcc
    13801 aagtatgatt cgattaatgg atcaagagtg atggtgtaca aatcagacaa attcattatt
    13861 tctaagatac gagctttttc atcaataatc gctttacgtt gcgctttagt cgtcattttt
    13921 gtatacaccc ccctttgttt tttgaaaaat ttgacctaac gatgcgcgtg actccccgct
    13981 accctatctc cccacgcgaa aaaattttga aaatgaatag gggggcttca attgaaatac
    14041 gaaggaaaaa cttttttgtc ttccgtttcg ttttcaacaa ttggatgaca ttttgaacat
    14101 aatagcatga gattgtttgg atcaagctta agcagttcgt tgtctttgat tggtacaatg
    14161 tgatggacgt gtgctctctt tccaaatatg aactgaccac accgctgaca atgaccacct
    14221 tctctttcat aaataaattg acgcatatct ttccatgctt gcgttcgata gaatggtttg
    14281 ttctcatgat gataaacaga ctttgcttgc tgcttcttct tgcgtgatct gcttgatctc
    14341 ttgtgttcag tacagtagat accctttgct attttgttcg tgcatccgtc aaactgacag
    14401 tatttcattc tacttcacga ataagattta tgatatcgcc ttttgcacgg atagcaccag
    14461 gaatatcaat accatgtttc ttagcatatg cacgcaattc ttttgcagtc atattgtcca
    14521 gttcatccgt atcttcagtg gactgatcat tagtagcttc gtctccatca aaattagcag
    14581 cagtgtttcc atcgctatca agaatttcac tgctatgagc tatgagttcg ccattgacag
    14641 caacaaatga tttgccatca ctcaaaattc ctttttcttc ttttacttca aaatttggtt
    14701 cttgatcttt cggaacaact actgtccttt tcttttctga atcccaatac tctgttccac
    14761 taatggatgt tctaattttg atcattgcca ttttaattct ctcctttgta atttgtacgg
    14821 attacttttg cgcccattcg ctcataccat tcaatcgttt cctgcagatt gggtagagtg
    14881 tgtgacaata aagaaataac taacgtattc attccagttc tgtaatcatc attctccact
    14941 tgcacatgtg catgattacc attccaattt tctctgtacg gactattctc tgcttcaatt
    15001 gctttcttat aaactttagc caaacttgat tctacttcaa tactaagtac cgcttcatga
    15061 aaatcattca tttgtttact ctcctatcag atattttgtg gtatgattta taaaaaagat
    15121 gcgaggtgtt ttaatgcttc aagaagttaa tattaagtat cgtgatcgtc aaaatacaga
    15181 gacaatttat ttgccaaata gttgtccgca ttgcggaaat ataatgactc cgtatgtgta
    15241 ttttggggtt tcatcatata gtgcgttcga taatgataga atctttggcg tattagtaca
    15301 gtgtataaat gatgagtgca acaaatttta tagtttacaa tttataaata cgtttaatga
    15361 agatgaagga tattctgaaa atattttaat taaagattat taccgtccgc cgattaaaat
    15421 agatttacca gaaaatattg aaaaagtttc tcctacattt gttgatattt atactcaagc
    15481 cacacaagct aaatctgagc aattggaaca aattgctgga gttggttata gaaaagctct
    15541 agaatttcta attaaagatt atgcaatatc taaaaattca gaggatgaag ataaaataaa
    15601 aaaaatgtta ttaggtaaaa caattgaaaa ttatttgaat gattttccta aactccaaag
    15661 attagccaaa gctgctactt ggattggtaa tgatgaaacg cactatgttc gtagacatac
    15721 taataaagat gttcatgata tggatgcatt tattaaatca gcagcgcaat ttatcgcggc
    15781 tgattatgat gctgatttag ctgatcaatt tataaatgga aataacttta tgccaactac
    15841 taattaaaag aataaaagag ccacagatac caattaataa aacggttggg atatcttggc
    15901 tctttcatat ttttttgaca ctaatagaat atcatgctgc taacaatgtt tacatagcta
    15961 caatgaccga acatttagtg aacagtgtaa ttttagaata aagagagttg ttctttgtac
    16021 ttctcgctat tgtaaaaatc aatttcgttt tcttttttca atcggtcagc ttgtctgtgt
    16081 tcatactcat ctaaaaagtt caatgtcttt ctaatttctg catgtcgctg tctgacatac
    16141 gatgagctat acccaacaga ttcagcaata tcttctaagc tcatttgatc aacatatttc
    16201 atcttaacaa tttgattatc gataccagaa aagctatcta tcagcaataa catttcttct
    16261 ttttgctcga tcaacaattc caattcattt tctatctttt ggatattctc ttctaaagaa
    16321 gaagttctcg aattcttttc gatgcggacg tttgctaaat caccacagac ccatcgattc
    16381 aattcaagct tacttttatt aagattccac tttaagtaaa gaatctgctc atctagttct
    16441 tggtaatctt ttaaccaatg aaatctcaca aacgccaccc cttatggtag aatagtattg
    16501 cgctgctatc tcgaaagggg tagctttttt attttttctt agccgttcca ttctggattg
    16561 ccttttgttc caaacgtcgt tttttcttct taatctttga ttttgttttt cccatacaat
    16621 tctcctttcc gaaaacacat tttgttcgat ttttaatact aaataaaagt tggccagaat
    16681 ttttcgttcg tctttactca tctttttttg aataagatta ctagcataaa aatagttagt
    16741 accacgaaaa tgaacaggta aatcaattac gttcaccttc ttcatgccag ttatcagcca
    16801 ctagaatctt tgcgttagtg ccgccaccgt tttctttttt gtatttttca aatgctttat
    16861 aagcagctgt ttttgattta tagatttttc cggaaccgac tgggaacatc catgtgccct
    16921 ctgtgatagc taagatgtat actttcattt aattcacccc tttaatcccc aacactacat
    16981 atccggactt ctgcatgtaa tcagtaatat aagtgatctc tgctttgtat gagtcaccag
    17041 tgtaactaaa tgttggtctc gttagtccag gtaattcatg tttaatgtat tttttatatt
    17101 cacgtagtat taactgatca ccaactttga agtcacgatc attcttgcgg atctcgaatt
    17161 gtttgcgtcc gctcgtcact gcttcaaaat attctggtag tatttttagt tcgtggatca
    17221 ttctgtttcc tccttcaaaa ttcgctcatc atcaaaaagt gaatctccac aaaatccttc
    17281 tcccaaatat ttatcgttca tgtataagtc aaccaaatat ttgtgatctg cttctcgata
    17341 ttcattaata ttaacgatct ttattttgta aacattatct tcgtcgctca ctatcttgaa
    17401 ttgttctctg actttcacca ccgttcctcc tctttttgaa aaaattttag tgtttttgta
    17461 agacccccta aatcagcttt tttatttgat ataatacttg tatcgattaa tcaaatcgaa
    17521 attaaattta aaaaggtgat ttatcatgaa tatcaacaat ttagtaaaac tcgttgaaca
    17581 acgttttaaa gaatcaggat tagaacaatt aaaaaaagat aattctccag aattattttc
    17641 taattttacc gaagacgaca ttcctactct taaacaatta aaatattatt ctgattattg
    17701 gacgcaaact cgtgagaatg aattaaattt agaaaaactc attattaaaa agaaatagca
    17761 actccagccc acacggctgg ttttttagtt tcaacttccc tccttacggg tcaaaatcat
    17821 cggttccggc ataatctgat catcctttag caacaaatca ggatattttt tccttagctt
    17881 cttattgttc gtgtaagttt tcaatagcca ttggttggct tcgcttttgc tttctccaga
    17941 aaaataaatt ttccctgttt ggatgcctgc aagttgaatc atcattttca cctcaaatca
    18001 aatttttttc tgctagatgg agcaaaagca atccgacatc aagaatcttt tgacccatta
    18061 tttcagctac tttgctcggc ttaactcctt gagaaaagag caagcgtgct tgctctatct
    18121 cttcgtgagt ccaaataaat ttggcttcct caaggataat tactggatca gttgctctca
    18181 ttgttcctct ggaaaaatga tgtcattctt atgcttggac caggtatctg catatggaac
    18241 aaagtattgt ctagctaatt cgatttgatc aattaaagca gtttctgata attcatggtc
    18301 tgcagcaatt tcattcatac tatcgccttc atctaaacag ataagaccgt tgacgttatc
    18361 gatttttaca tttgtgactg cttgactttc tccacgctca taatattcta cagttgatac
    18421 tgatacatca gcttcggagt ttaattgcag aatctgttac tgccgggata atttaatatt
    18481 cttttcataa ttcacctttt ttcctactga catcgaatat ttttcatgtt atgattagcc
    18541 gtgaaaggag agctatatta tgtgtttaca catgatgtcc ttagctcatt cggttctttg
    18601 gaagcttttt tggaaataac tctcaggttc ttgacgcaaa tctcatttat tctttgaaac
    18661 aaaaaacttc cattttccga gagctttgga tcacttcaaa agagatttgc atttatattt
    18721 ctgtgacctt gagtcgcgta aagaacctta cagcaatggc ggcaattcca ctttcaggaa
    18781 accgcctttt ttcattgata ttggtggtta gcggactacc cattttcttt cggcagtgcc
    18841 aaaccacaaa tgatctgtgt tgatgtttag gtagttgtat accagataag ctaaatccaa
    18901 agtgcctgca tcttcaagac gacaatattc caaaaattct cccatagctg gacataaaaa
    18961 tcctggagct atcatatccc aacagttaat agtgcaaaga aattcgttta gcttctgaac
    19021 gttttctttg gtttcacgaa ttacaacata ctccaattct tctccgctgg cagagcattc
    19081 caaaacttca aattgattaa gaaatggtag aacatatgct tcaaacatca acattccatc
    19141 tttctgtttt tgcacttgtt ttgcatcaat catgtctatc ctcctattca gttatttctt
    19201 ccattaatgg aattatttca ggtcactgga cttaacgaaa acaccattga ccatctttcc
    19261 agtccgacct ttgatttcgt cataagcaaa atccaagcac tcgtataaat ccatatcatt
    19321 ttgcattgcc aagatgatca aggttactac tacatcgcca attccgtctc ttaaaccatg
    19381 ttcatctttt ctagctagag aagcggcaac ttccccgatt tcttcgaccg tttttaacat
    19441 ttgcttgctg aaatccgctt gatccaatcc cttatctttt gcccactgct ctactttcgc
    19501 gattagttcg tccattattt ctcctccaca tacctaaact gtcttccttt tgaatcaatc
    19561 cataagctcc tagctctatc ccaaataata tttttgctca gaccagtaat ttcagataac
    19621 tgtttagcgg tacccgttac tagaattcga tcaccatgcc agattgcaat ctttctcggc
    19681 gttctccgtt tgggcttttc agtccacatt gatttaccga gcttttggac ttctgcaact
    19741 atttttttat cttcctgcca atcttctgaa taagtcaatt cgataattcg ctgcattgcc
    19801 gctttcttat ccatcccgac attctccttt cagtaatttg agtacttgat caagtgcgct
    19861 ctcacgtccg ccatgaaatg tgttgagcca cttgtcttcg tacgacacac tttgtcttaa
    19921 agcttcttga tgcattaatt cgatctgtgc tgtaaatgtc tttaggtcca tcttgtttac
    19981 acctgctcaa gttcattaag atgtttttgc aagcctttaa cacaatcaat gaatagtaat
    20041 ttggtatatg ctaaatttct taattgcgtt ccatcgatat agagtgcgaa atagtatctg
    20101 agtttactcc aactcgaacg atcattctta attccttcaa ttccagcttc ttcgagttga
    20161 tcatatacgt ctctcaggac gtctaattcc tcgccctttt tataattcgc tatttcatta
    20221 attagttcta gataatcgat cttcattttt ccacctctta gaatggtgct tttgattgtc
    20281 tattagctcg ttctagcgct tttttcttga gataggcttc ttggtcgatt gcccattcag
    20341 gaagcttctc tcgtcttcct gtgcgcttgt atccactgct tgcgttctta ggttcacttt
    20401 tttctttcct tgcccaactt cgaatagttg ccaaatagtt tttataagtc ttaccagatg
    20461 attcacaata ctctgacagt cgttcgattc gctcttggta atcattaggg aattctgttt
    20521 tgagtttctc catctgctca tctgacaaaa gaacattttt atactctccg tatttatgac
    20581 ggacgggctt agccttcgat tttttcgaag gcggtaactc tcttatatat tcttttgtat
    20641 tattaaatgt attattaata gatgtattat tatctttgac tttttcgtca ataggggtat
    20701 tgcgtttttc gtcaataggg tattgattaa ttcgtaggta cctattgatt atttgattgg
    20761 taccctcttt gtaaatgatt tcccgattca agtatccaaa cttaatcaaa tcacttaccc
    20821 atcgcgatat ggtctcttta ttcacaccat ataaatctgc aaagtactca ttgcctgccc
    20881 aacaaaagcc tctttcatta cacaaggccg ttatctctcc gtataacaac ttagtatttg
    20941 gtttaagtct tttgtcgtac cttacgttgg ctggtataat cgcataataa cttcgatgtt
    21001 ctgtcatttt taccctccaa tatttaactt tttgattgtt ttctggttta atttgatccc
    21061 tttgatttga tatttatttt tgaaattaat cacacctatt ttgtgcttct ctgtgtgatg
    21121 gattctgcag agtgctgcaa atgtgtactc tgcatgatca acttctttgc gctttcgtct
    21181 tcctagcgct ttgtcaaagt gatcgatgtc agctcctgtt ttgccacaga tgcagcagac
    21241 tctttttgtg atgcatttgt agaagtaata ttcttgattc gcaggtaaaa tctcatagcc
    21301 ttctttgaaa ggaatatgat gttcaaagat gaaatctaag atgatatttg ctaagacatt
    21361 ggcatcactc acagttgtat tcgattcgtc tttcaggctt attttgcgcc ctgtgacacc
    21421 ttcaaaacga aagtagaaga attccttcca gaagtccgtt ggcatgcctg tatcgataaa
    21481 aatatcgcct atcagcgcat agatgaagtt tcgttgctgt acggtgaaac gtctaggatc
    21541 aataaaacga atttcaatga ctcgatcacc atcgtagccg tcatacatcg tctttagtcg
    21601 atcaatgttc acttcctcat tgatggttgc gcttatgtct tttcctttga actttttcag
    21661 aaccgctgaa tatgaatcga ttaatggttt aaacactcat atcacttctt atctaattct
    21721 tttctcttag ctgctattgc tcgctccatc aaggcacatt gctcatagct taactgttca
    21781 atagtttcaa cgttatcagc taagagccct aatttatctg tctgctcatt aacatattcg
    21841 attaaggttt tggtcatatc tttacccatc tgctcattga aagcttctag aatcgtctct
    21901 agcatgttta atttctttgt atcgattcta ggtggtgttg gaatatcttc cccttgaaat
    21961 acatataatc ccagtccgtg tagagccaat gctttcacaa agcatcgctt caatgagtta
    22021 ttgatttgca ttgcatttgg tttaacaact ggttggtttc gataatctaa aacaggaaat
    22081 aattcggttt ccgtgtgtcc tttaaccgtt actgagacag atacataagt cccagtttca
    22141 tccataagaa aaggtttata ttcctcaaca agaaagtctt gatgagttcc agaaacaacc
    22201 ctgtagtgtt tgtactcatt aatagttacc gttgcctgtg gatcattctt tttcataatc
    22261 tcccacgcgt gagcccaaga taaataatca aaatttcctt ttttcttgag aattttattt
    22321 aacttacgac taaaaagttt ttcaaagttc gttgtccctt tgatttcact catcaaattc
    22381 tgcctccatt tcagcaatgt atttcttacc tggtccgtaa taagagatat caatcaagtt
    22441 atctctgtcg tactcttcta gcgcatcaat caagccatct tcgatgacgt aaatatattc
    22501 aggtttattc gaatgcttcg atagatggat aagataaaca tgatcccaaa tagttacatg
    22561 gttgcctaaa tcatcttgat cccaagctag ttcttcattc gtcaaaagat ttcgtctgat
    22621 ttttcgacca cttgtttcct caattttcgg cttgccccaa tcaggatcaa tcaaatattg
    22681 atctagagtg gaaagttctt tttccatatg ctaaaatctc cttatgatgt gttttctttg
    22741 tgactctttg cttgccggcg gagtcacttt tttatttgtt gccatgcttt ttgcttgtca
    22801 atatgttgtt ggcttaggat gattggttta tagtatttcc accagcaatt agcaattgcc
    22861 gtccctattc ttagcgcttc agctctattc attgtcatca ccaaaaagtc tctgttgtct
    22921 gttcagttga tcaatttcca tacggatcgc agtttctggc aaccacattt caataaatga
    22981 aacagcatca tcgaatctct tacgaggtaa ctcgccatat cttgggattg aaaaggtacg
    23041 tttaaattca gaccaaaatt ttgagaatac ttttttgctg atttcttcat aagctcggct
    23101 ttcttttccc cctagaactc ccataacttt catatttcct ttttgcttaa tttcaaactc
    23161 ttgttgtccg ctaattcgca tagtatcttt aagcatggaa acatcttttt taacatcttt
    23221 catttcttct aattgataga tcatcatatc ttcaattgtt tgaggaacag tattcttgcg
    23281 aataacatct tccatttcgt tgaatgcttc aatgtatttt tgtttgaagt agatagcttt
    23341 ctttcctgta aaccccatag ccagcaagaa aaatccatct ctactaatga agaaaactcg
    23401 tcgatttctg ccatatgaat ctggttcatt accttctaca aacatctgtc caaaattgga
    23461 cacatctttt tttagtgcat caatatctct taaaacatgt tgatgttttt tctcgaagct
    23521 ttctgccact tgtaagctcg tagtcacagc ttctttattt ttcaaaatta ctaattcttg
    23581 cattatttct tctctccttt ttggtataat ttagttaaaa actggtggtg tttattttga
    23641 atttttttca cttaacaatt tttcaatgga tgtcaataat aagttttata ttgagtacgt
    23701 ttttaacagg gataaaaata aaaggaatgc gccctcaact agaagttgaa cttaatgctt
    23761 cttacttcgc tgcagatatg atttatacaa aagtaatcat ttctaactat tcaacagaac
    23821 cagctatgtt agttaattta gaactttcat ccgctaactt aaaccgcaga tggtcagcta
    23881 ctccatttaa gaaattaatt gctaaaggag gatcagtcga tgataataga atatactctg
    23941 aagctgtacc gctaaatatt cctcctaaaa gtgctatatc ttgttacctt gcatttgaag
    24001 tagggaaaat taattttaat aaactcctta atcaccaaac aaaaatgata tttactctaa
    24061 atagaaccca aatccacaaa attgttgata tcaaaaatac aaacttccca atagagaaat
    24121 tagtgaaaga attaaactga gtaatagtaa tatacattct agtttctctt ttctattcat
    24181 ttttagtcag ccccctcggt tggctttttc gctctgtact cagcttcagc ttcatcaagc
    24241 cccataaaaa tccaaaccat gtaaacaatc gttcctatca acgcttgcct gcttccccaa
    24301 agtcctaaag cgtagatgat tagcggtgcg ctgaacacta gcgctctatt aaattttccc
    24361 atccgcttgc ctccttaaga tttccgaaaa atttgtttct aaaaattcaa gtgttttact
    24421 tcttaaaaat agatatgtgt ctcttccttc aactggataa tagacaaatc catttttgtt
    24481 tttctcgata tcgataatat ttctatatct tgggttcttt aaaactctag aagtaaacca
    24541 atcatatttt ctgttaatcc gttctagcac ttctggcaac gtcatccatc taccagtatc
    24601 atcagctttt tttaactcct cataatccac ttgggagata attacatagc cttctggaat
    24661 tgggattttt gcttctagat attgcattag ctgttcctcc ctttacaatt cgtacatagt
    24721 gataatcgaa tctataattc tgtttgcttc tgcagaagtc tttttaccgt ttaaaattaa
    24781 agataagtag cttttgctaa ttccaaatct ttcagcaagc atggtgtaag ttaagaactt
    24841 tgaactttcg acatattctt tgattttttc tctatctcgt tgagtgattt ctgcaatgtc
    24901 agtcatacta aaactccttt ctaaccaatt tcctctaaat ccatttgagg gtaatatcct
    24961 tcttttttta gtaattcgta gataaataga cgccctttct gtgtccattt ggtattcatt
    25021 acaattttag tgccaccatc ggctttcggg atctcagttg tatgagattt tgtgtatcct
    25081 tggttcatgt gttttttgca caataaccat tggttaccga cttttttctg aatacctagt
    25141 ttatgaagta atttattcat ctgttgtgga gacatcccat aatctgctgc aatctgacta
    25201 attgttactg aatctgtaga agataatatg ctatctaaat aggagatttt aggttcgtac
    25261 tctgcaatct tttgttctgc gattaatctt ccagttcttt cttcttttag ttgagttgct
    25321 aattgaatga ttgtatctgg attaagcaaa gcttcttcta ctttttctgg agttagataa
    25381 cctccatgtt ttctaattgc tggcaacact tcacttgtta cccaacgttt aaattttttg
    25441 gcagagggaa gttttgattt taagattaaa ctgtacaaac cagattcgtt gatgattgtc
    25501 atctctcttg attgacctga ggtcgtgatt cgcgacccca tcttatcttc caaatctaca
    25561 tgccgcgaca aagcatcttt agtgtttgaa taacccaaaa cactcgcaac gtctttgcct
    25621 acaaaatatg gttcatcatt tactaaaatt gtccgaactt cgttttgttc gaaattaaaa
    25681 atttgcggtg tgttcatatt tctcattcct ttcttttata ttcgtaaaca aatttaacaa
    25741 cttttttcta aattcaattg acagtatctg agtaatactc tatactaaga gcataattaa
    25801 ataagacata aaaacattga ttttaaagct ttcttggcgg tctgcattta catatcaata
    25861 gtgttttttg ttgtcttttt agttgttaaa tctgtttaca agatatagta tagctcatta
    25921 ctcagaatta gtcaaattat ttctgcgtat ttttctaaac tttttttgta aacatttaga
    25981 aaggttgttt tatcgatgaa tacctatgaa ataataaaag acttggcgaa gaggaaaaag
    26041 atatctatac ggcagttaga aattaatttt ggatattcta acggttatct agcaaagtgg
    26101 aaaaataata ctcctaattc agatgaatta agtcggattg ctgactattt cgatgtctct
    26161 gtagactacc ttctaggtag agaagaaaga gaaaccccta aacatgtgga tttatcagaa
    26221 gacgatactg ttttttcttt tgatggaaaa gaaatatcta aggagacaat gcgtaaagcg
    26281 attgcaattg ctaaagcttt agaggaaaat gaatagttgg agtgatggtt tgtatgtatt
    26341 tgaaattgaa agaaatgttg agcgaataca atttgaaagt agtttatatg gagatgaaag
    26401 agcctggttt ctactatcca aaacccagaa tcattttttt gaatgaaaat ctatacggtg
    26461 aaactgcaga agcctttcat ttatctcatg aacttgcaca tttcagcgcc tctcattttg
    26521 aattttcagt tttgtacgat acttctacaa cttttcactc aaaatttgaa acagaagcag
    26581 ataaaatagc tattctaatt ttattaaata tctatattga gaacgaactg actgatgaat
    26641 ctcagtttaa tctcgaaaaa tttatggagt attattctat tcagaataag ctcagataca
    26701 cttgttatgc tgtctgccaa tgctatttta agaaaaaata ttcttacgca aggcaatatg
    26761 tatgaataca tcaagaatta ttaaaaaaac cgatttcagc aaaacattaa aaaagcccgt
    26821 gctgcaacac gggctctttc ctcatatatg agcttctaca aaaaaaatca tatcacagaa
    26881 atgaggaaga aaaaatggaa aaagagattt taataaaatc tccaggaaaa accttgatta
    26941 aagtaactga tgattctatt tcaattatta gaaaaggatt tataaattta gttaatcaag
    27001 gtattaaagg agaaaaaact atccccttta aaaatatatc agccgtacaa ttaaaaaaac
    27061 caggtatgag taatggttat atacaattta ccttgctagg cggaaacgaa agtcgcggag
    27121 gtatattagc ggccacgaaa gatgaaaaca ccgttatgtt tacaaaaaaa tattggaatg
    27181 aaatggaaaa tttaaaaaaa tatatagaga agcaacaatc tatattagat aataattcca
    27241 aagaaacaca aatttcaagt gcagatgaaa ttaaaaagta taaagagttg ttagatgaag
    27301 ggattattaa tcaagaagaa ttcgatttta agaaaaaaga attgcttggt ttataatatt
    27361 caatatcaaa agagaaagag gatctattaa tggacggtat atttggtttt actggtattg
    27421 ttttatttat tataggttta atcatgttaa ttgtaagatt tatcaaaaaa acaaataaga
    27481 aaacacctat gattctcctt attatcggga ttatttttac taccgtaggt ttctcgcttt
    27541 cctcatcaaa cgaggcaaat gacaataaca aacaggaaag ctcatcatct gatactcaaa
    27601 ctactacatc taacagcagc aataaaagcc taactcaatt tgaagaattt ttagagacaa
    27661 ataatcatga ctggggtgct ttcttagata gttattattc tattactcca gctactgagc
    27721 aaaatacagc ttttactaaa tatattagtg gtaaaacata tacttttgaa ggaactgtta
    27781 tcgaatcaat gactactaga atagccatta tagcagacaa agagtacgat gataaaagtt
    27841 ggaatgatat ttctgcaaca cctaaagttt catatgtaat ttttgcgaag gacttaaaca
    27901 atgctgatac ctttacaaag ggcgataaag taacttttac aggagaaatt agttcaagag
    27961 gatcgaacat agaaaaatcc tatgctcaat gggatatgat taatagtaaa gtaagtaaaa
    28021 aataaatgaa ccacgcccca ctaagagtta gattgtgcac aataataaaa cctatacaaa
    28081 agagtgccat caggagtcac aatgcttgta aaggaaatga caaaatagga ggtaactatg
    28141 gataatagca atgaaaaact aaccaaaaaa gaatatcttg aattaatagt tgaaggcggt
    28201 attggagcaa ttccttatat tggtggcgtt gtgcagtcac tttattttgg gtctaaaaac
    28261 gagaagcgtt tcaaacgcat cgagaaattc tacaaaaatt taaacgaatc attagaaaac
    28321 attcgcgatc aaattccaga agatgttttc aacttagaga acaaggacca actcattgga
    28381 atattcgaag ggataaatga cgaggtagaa aaatcaaagg cacaaaataa aatagatatg
    28441 tataaaaatc tttataagaa ttgtttactt cgaattaaca gcgcttcatg ggataacgag
    28501 gagtattatc ttcttgtact gaatcaatta acatcgattg agattcaatt gttagcatgc
    28561 ttaatgagtc gtggaaacga aaatttcact ggaaatatca gcatgtctgg ctattcacaa
    28621 gagctaatag atggtagctt aaatagatta tctgattttg gtctgttaga aaaaacgatt
    28681 agctccatag tattaggtga tgtcggcaaa caaaatatgg gctacaaaat atctcattta
    28741 ggaattcaat ttattaactc aactcttact taagttttct ttctaatatg acatctagac
    28801 ctatcagcaa tgcaacaata aaaaaccaga cataccaata atcaacaaat aatctaaact
    28861 ctggaagttc agttattctt ttaaaaatca ctttaatcat aataaacacc tctgattgga
    28921 tgatctaata cacatccaat tataccatta aaacgacaaa tagcacatcc ttgcgccggc
    28981 aagcaaaaag gatgtgcaaa aaataataaa acccaaacaa tgggcttctt tatagttcct
    29041 attgtatcag agaaagagag ctgattcaat tatggcaaga ttagtcaaac gtggaaatag
    29101 ttggcaatac gaaatttcat acaaaaaaga tgacggaaaa tacacgaaga taagaaaatc
    29161 aggatttaag acaaaaggag aagcaaaaga tgccgccaac gaattagaat ataacttgaa
    29221 caaaggcctt aaaggggatc gcaaaaatct attattatca gattactttg aggattggat
    29281 gcaactttat aaagaaggaa cagtatctcc tatcacttat agaaaatacg aagatacgtt
    29341 aatgaacata aagaaatata tgccagcggt attgatttct gatttagata gagtgggata
    29401 tcaacgcttt ttaaataagt atgctaaaga ccatgtaaaa tctaccgtta ttaagtttaa
    29461 taaccatatt agagcgtcgt tgaaagatgc cgtagaagaa ggattaattc cgtttgatcc
    29521 aactagaaaa gcagtaatca aaggaaaaga ttcattgaag ccaaaagaag ataaatattt
    29581 agattatgat caatttaaat ctttaatgaa actcgtagaa gaaaaccttt ctgcgcagta
    29641 ctcttctcct atgctcgtgt tagttgctgg tgccactgga atgcgatttg ctgaacttct
    29701 aggattaaca tgggaagata tcgatttcga agatcaaatc atcactatta ataaaacatg
    29761 gaattataaa ttaaatgaat ggggaaaaac aaaaaacgaa acttcaaata ggaaaatttc
    29821 cattgataaa catacgattg atctcttaaa aaagtttaaa atcaatcaaa aagaattatt
    29881 cgagaatttt gaaatcaaaa accctcataa ttttgttttt ttcaacttaa aaaatggatt
    29941 agtttcatca aacgccgtca gcaaatattt gcgcaaaaaa ttaaaagaat tagggattga
    30001 aaagcaattt actttgcatg gactaaggca tacacatgca tctattttac tttatcaagg
    30061 agtaaatata cttagcgtat cgaaacgttt aggacatagc agtttagaaa ctacaatgtc
    30121 tacttatctt catattgttc gagagcttga agatcaggat aaagaaaaaa tcaatactgt
    30181 gttcgatagt ttatataaaa atgataacta gtttggcaca tttttggcac aaatacaaaa
    30241 caaacccctg taccgcaaag gatacagggg ttattt
//
LOCUS       DACCCS010000034        29112 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.034, whole genome shotgun sequence.
ACCESSION   DACCCS010000034 DACCCS010000000
VERSION     DACCCS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.034"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..1031)
                     /locus_tag="IVK82_001577"
     CDS             complement(<1..1031)
                     /locus_tag="IVK82_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6392645.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="IVK82_001578"
     CDS             complement(1113..1802)
                     /locus_tag="IVK82_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP6392646.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IVK82_001579"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IVK82_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP6392647.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="IVK82_001580"
     CDS             complement(2329..2832)
                     /locus_tag="IVK82_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP6392648.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IVK82_001581"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IVK82_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP6392649.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IVK82_001582"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IVK82_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP6392650.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="IVK82_001583"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="IVK82_001583"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP6392651.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="IVK82_001584"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="IVK82_001584"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP6392652.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="IVK82_001585"
     CDS             complement(6444..7463)
                     /locus_tag="IVK82_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP6392653.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7637..8524)
                     /locus_tag="IVK82_001586"
     CDS             complement(7637..8524)
                     /locus_tag="IVK82_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6392654.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8601..9146)
                     /gene="nusG"
                     /locus_tag="IVK82_001587"
     CDS             complement(8601..9146)
                     /gene="nusG"
                     /locus_tag="IVK82_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP6392655.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9247..9417)
                     /gene="secE"
                     /locus_tag="IVK82_001588"
     CDS             complement(9247..9417)
                     /gene="secE"
                     /locus_tag="IVK82_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP6392656.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9441..9593)
                     /gene="rpmG"
                     /locus_tag="IVK82_001589"
     CDS             complement(9441..9593)
                     /gene="rpmG"
                     /locus_tag="IVK82_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP6392657.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9755..10399
                     /locus_tag="IVK82_001590"
     CDS             9755..10399
                     /locus_tag="IVK82_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6392658.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10455..10958)
                     /locus_tag="IVK82_001591"
     CDS             complement(10455..10958)
                     /locus_tag="IVK82_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP6392659.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10971..11017)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11555..12520
                     /locus_tag="IVK82_001592"
     CDS             11555..12520
                     /locus_tag="IVK82_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP6392660.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12619..13791)
                     /locus_tag="IVK82_001593"
     CDS             complement(12619..13791)
                     /locus_tag="IVK82_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6392661.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13778..14923)
                     /locus_tag="IVK82_001594"
     CDS             complement(13778..14923)
                     /locus_tag="IVK82_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6392662.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14937..15869)
                     /locus_tag="IVK82_001595"
     CDS             complement(14937..15869)
                     /locus_tag="IVK82_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6392663.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15986..17065
                     /locus_tag="IVK82_001596"
     CDS             15986..17065
                     /locus_tag="IVK82_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6392664.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17062..17691
                     /locus_tag="IVK82_001597"
     CDS             17062..17691
                     /locus_tag="IVK82_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6392665.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17801..18028)
                     /locus_tag="IVK82_001598"
     CDS             complement(17801..18028)
                     /locus_tag="IVK82_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6392666.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18131..20719)
                     /locus_tag="IVK82_001599"
     CDS             complement(18131..20719)
                     /locus_tag="IVK82_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP6392667.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20739..21398)
                     /locus_tag="IVK82_001600"
     CDS             complement(20739..21398)
                     /locus_tag="IVK82_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6392668.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21447..21950)
                     /gene="trmL"
                     /locus_tag="IVK82_001601"
     CDS             complement(21447..21950)
                     /gene="trmL"
                     /locus_tag="IVK82_001601"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP6392669.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22171..23037)
                     /locus_tag="IVK82_001602"
     CDS             complement(22171..23037)
                     /locus_tag="IVK82_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP6392670.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23146..23787
                     /locus_tag="IVK82_001603"
     CDS             23146..23787
                     /locus_tag="IVK82_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP6392671.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23849..25210)
                     /gene="mgtE"
                     /locus_tag="IVK82_001604"
     CDS             complement(23849..25210)
                     /gene="mgtE"
                     /locus_tag="IVK82_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP6392672.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25228..26127)
                     /locus_tag="IVK82_001605"
     CDS             complement(25228..26127)
                     /locus_tag="IVK82_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP6392673.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26129..26926)
                     /locus_tag="IVK82_001606"
     CDS             complement(26129..26926)
                     /locus_tag="IVK82_001606"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP6392674.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26904..27608)
                     /locus_tag="IVK82_001607"
     CDS             complement(26904..27608)
                     /locus_tag="IVK82_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP6392675.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27739..28314
                     /locus_tag="IVK82_001608"
     CDS             27739..28314
                     /locus_tag="IVK82_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP6392676.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28432..29109
                     /locus_tag="IVK82_001609"
     CDS             28432..29109
                     /locus_tag="IVK82_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP6392677.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8568 a         6169 c         5232 g         9143 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa aagtaagcaa acatttactc
     7621 tcagatactt ttatcgctat tctttccaaa taaatcgact tacaagctga tctacttctt
     7681 ggttttcatg aagcatacta tgagaggcat tttcacctgt cactactttt tcctctattt
     7741 catttccgcg ttgcttcaac aacgcataga ccgctaatgc actgtttaac ggtactgttc
     7801 catcactcaa gtctgtttca tctagttgac cagcaatcaa gaaaaatcga gtggttatag
     7861 gaatattacc gatcatttgc tggtaatctt gatagcggtt agaaacgaca gcaggcccct
     7921 ttgctaactc atctgtcaga gactgcgcac tatcatcgac aaaatcatta aaaggcgcgc
     7981 caattgctgt taatgtctga acttgtggca atgacgtatc ctgtccatac gttcctaaat
     8041 agcgtagtgc actaactcct cccatggaat gccccactat gttgacttta tctatgccgt
     8101 agttttcttt cagatacaaa agacaagttt tgatccattc tgcttgattc cactcgttat
     8161 ttttattatc ttcaaacaat acttggacca tcggattgtc tgcttttcca gacaaatctc
     8221 ctgttgcttg tatttgtcca tctgttccta ctgttaaaac caattctttt actgcttgct
     8281 gatttttttc caaacgccgc aacatccctt taaaggaatt ttctgtcccg ctgtagccgt
     8341 ggaaaaaaag tgttggaata gtggtttttt ctatcacttt actggattct gccgtttttt
     8401 ctttatcaga aacttcagta gtatttatct tcgtcgtctg gcaaccactt aacgcaaaca
     8461 aactgataag gactaaagct gctgcagttg cacgtttgca taaatttttg attggttctg
     8521 tcatacacct cttcctttcc atatagaaaa aagaaagact tgaaaggcca atcatatgat
     8581 cgacatttca agtctcttta ttaatcgatt ttatctactt gatcgaaatc aagttctgta
     8641 ctggtttcac gtccaaacat atcgatgttg actttcaatt tttctttttc tccgtcaact
     8701 tctgttactt gtccttctaa tccagcaaat gcgccttcga tgattttcac gacttcgcca
     8761 acttctactt ctagatcatt ttgtcgcagg ctcataccga tagaacgcaa gatatgattg
     8821 acttcttctg gaagtaatgg tgctggtttg cttccggctc cgtgtgaacc aacgaatcct
     8881 gtcacaccag gtgtgttgcg gacaacatac catgaagcgt ctgtcatgat catttcaaca
     8941 agtacgtatc ctgggaatgt tttatgaacg atttcttttt ctttcccatt tttgacttca
     9001 gtttcttttt cttcaggaac aacgacacgg aaaataaagt cggccatttt catactttgc
     9061 gcacgagatt cgatatttgc ttttactttg ttttcatatc ctgaatatgt atgcaagaca
     9121 taccagtttt tttcaaaaga ttccattgaa ggctcctcat ttcattgtaa aataaaaaaa
     9181 cctcagtaca cccgaagttt ttctttcgct tcattatagc actaaaagta cgttgtggct
     9241 agcgagttat tttaaaatcc agccaaataa gctttggatg gctgtatcca ttacgtaaaa
     9301 catcaatccg aaaataatag aagtttcgat gactactaac gtatctttac ggagttgttt
     9361 tctgcttggc caagttactt gtttgatttc atcttttacg ctttttagaa atttcatgag
     9421 acaacctccc ttgctactgt ttatttcgtt tctttatgta atgtatattg attacaatat
     9481 ttacaaaact tattgatttc caaacgttcg ccgctttttc cttcactgac tgattttgtg
     9541 tagttgcgag acccacaaac ggaacaagcg agtgctgctt ttttctttgc catttacttc
     9601 tgcccccttg ctgagaatca gacttatact tcataacagt atcacgtaac tttttacttg
     9661 tcaatttgtg aaataaacta tctattgttt tggataaaat tatgatatga ttaatttgaa
     9721 attaagctaa aatgagacaa ttggaggtat agcgatgcta ttaaaaacac ttgtatataa
     9781 gaaaaaagat ctgacaaccg ttacagagac tagtacatta gaagaagcat taaaaatttt
     9841 agaaacttcc ggataccgtt gtgtgccgat cttagattct tcaggtaata ttttccgtgg
     9901 aaacatttac aaaatgcata tctatcgtca taaagcaaat ggcggtgata tgacgcttcc
     9961 tgtcactcat cttttgaaaa acgctacaaa gttcatttac ctggatactt ctttcttcaa
    10021 agtattcttt acgatcaaag agcttcctta tattgctgtc cttgacagtg aaaatcattt
    10081 ttatggtatt ttgacacata gtacgttgct taatatgctc tctcaatctt ggagcgtaga
    10141 acaaggaagc tatgtactga caattgcttc cacaggaaag caaggagatt tggctgctat
    10201 tgcaaaaatc atttcaaaac acagcagcat tgccagctgt attactcttg atatcgaaaa
    10261 agacgaatac attcgacgta cattgatcac gctaccggct aacactgaag cgaaagtatg
    10321 tgaaacgatc gtcaaccact tagagaaaaa gaattataaa gtggtagaaa ttgaaaattt
    10381 gcaaaaccaa gaagcataat caaaaagcga aaaaagaaaa cagctaatct gctttctttt
    10441 ttcgcttttt ataattagac acgcttttca aaatcaagcg cgcgatctaa ataatacatc
    10501 aatggtgctg aaacagccat gacgataaac tcacttaatg caagcgtgcc ataggttgtc
    10561 caaaatgctt ggccacctgt tggaactaac atataagcaa tcagtcccat gctgatggta
    10621 aagaagatcg tgttcaatac taaacgcatc tttacatttt tcactttgtt ttgcaataaa
    10681 gctgtcaggc ctaaagaaag caatgtttgg ccgccaccaa aaaccacatc catgatacca
    10741 aaaccaaaga aggcattata gacgatgacc ccgcctaaaa ctccccaaag caatttacgg
    10801 ttaaagacaa ctaaatgatt caaactttct gaaatacgaa actggatcgg ccctgaggat
    10861 acaggcgcaa ctaaaatagt taacgctaga taaagagcca tgatgatgcc atttacaacg
    10921 attacggata accgttgtct gcttttcgtt ttttgcattt tctctcctct tttctcctag
    10981 tttttttaac gcgggatggt tgatgaaccg cggttatgtg aaacaaacaa taattataac
    11041 agacttttca tttatttcaa tccgtcttat ctgcttttaa aaaagcaaaa gcttaaacaa
    11101 tgtaaacgtt tgctaaaaat cgtgggatat ggtataaagt aagtgtaagg ggtcataaga
    11161 attcccagta aagcagacga agtaggatct gcaagaacag aattgttgtg aaacttaaat
    11221 aaataaaaaa ggatgtggga atttgagcaa cctcttacta gagaacggtc tagcaagcag
    11281 ctgaagatct aagtggacat gccgaacctt ggaacttctc cttacgtaag taatggatca
    11341 tgcgcttgct aggctttttt gtgtttttcg gtcttcggac ctttttcatt ttcctagact
    11401 tctgctataa taaccaagaa ttaattttgt aacagatttt tttgaaaaaa caagtttctt
    11461 tcattcaaaa aatagaaaaa gcttgctaat tattggttaa ccagcaaaca ggagtattaa
    11521 aatatcttgt tttattgaat agcaggagga aatcatgaaa ttccattatc atatattgat
    11581 caatccatcg gcaggcagcg gaaatggcta taaagtagca gaaagaattt taccggtatt
    11641 aaaaaataag catattgact atacacttca ttatagtgaa tacaaaggac acgatgctga
    11701 gattgctgaa actttggcaa aagaaacatt gattccttgg atagaagaag aacagaaaac
    11761 aatcgatact ttcccccttt tgatcatcgt aggaggtgac ggcaccttac atcaggtact
    11821 tgatactttc tatcagatgg aagtagaatt cccagttgct tatattccag caggttctgg
    11881 aaatgatttt gctcgagggg caggccttcc aaaaaatccc aaaaaaggac ttcagctgat
    11941 tttagcagct caatcaccag aaaaagtcca cataatggct tatgaagaaa aaataagtga
    12001 aaaaaaagga atagctgtta ataatttcgg tataggatta gatgcagcta tcgtccatgc
    12061 gacaaatcat tcttccacaa aaaagcgatt gaacaaatac aacttaggat cattttcgta
    12121 tttattctct attctccgtg ctttattcac tcaaaaagga tttccaattc tggtagatgc
    12181 aggaggaaaa caattaagtt tttcaaacgc atttttatgt acagcaacga agcatccttt
    12241 ttttggcgga ggaatcgcga ttgttccaac tgctgatgcg tctaaaccag tcattgattt
    12301 tgtcgtggtc gaacgaatca atttctttaa aatcttgtgg ctcattcttt tacttataca
    12361 gaaaaaacaa ttgaaatcga aatatttcca tcactacaca acaaacagat tacgtatcgt
    12421 ttcaacaaca ccgcaacatg gtcaagaaga tggagaagaa atggaaaaac gaccttttga
    12481 tatcacaata accacaacta aacagttgtt ttggtgttaa taaaagaggt tttgtcagaa
    12541 gctacatctg gaacaccgtt tattcggaaa aaaggactgt gacacgactt ttgtcacagt
    12601 ccttttttta ctatgcagtt aattctttgc ccaattgggt tgccatcgat agtttctttc
    12661 tttacccaaa ataaattgga tcattgaaaa caccgcagca aaagcaacta agacacaacc
    12721 attgaaccaa aaagattctg caaatgtttg attgtaactg agtgtttcac tgatcaagtt
    12781 attattttga acaaaccaat atgttggtgt gaatgccgct attttgctaa ccatttctgg
    12841 caagtattct gctggtacaa acaccccacc gataaagcaa ctgcctgtga taaaaatatc
    12901 tttgatccca ccgctggttt ctgagttttt taccaatgaa gtgatacaag ccgaaaagct
    12961 aatgatcggc aataaaaaca agaaagtatt taaaatatga taccataccg ttatatccca
    13021 cgaggtgtgg atcactaaca gaaccattag aagtaagaag ccactgaaca acagcagcga
    13081 gtaactaatc aaactaaagg ttactttgcg attcaagttt cgtttggtaa tgggcgcact
    13141 ctggtttcgt ttactgatct ctttgcgatt aaacgctaga ttgatgacgc tgaaaccgct
    13201 aaaaatggat agaaacaacc cataagacaa gaaattaaag gttgtgcccg tcaacagctt
    13261 tttcatccgc tgactatatc ccgcacttag ctcaacttct ccagtaaccg agagtgccat
    13321 ttgtgtctgt tccatagctg catcgattgt tttatcttct tcttgaaaca gaatcagtgt
    13381 ttgcaaaaag gtgttcactt gctgtgtaac aactgtttta gaaaaggcat ctgggcgact
    13441 ctgtatcttg atcgtcgcca tctttcctac ttcgacttgt tcggtaaagt ctgcaggcag
    13501 atgtaagata atgtttacac gattaaaata aagagcatca tcgatttctt tttgtgaggt
    13561 tccatccaat gtaacgactg tttccttgtc attcaaatat ttacgaaggc catcgactac
    13621 aggcgactct tcttcactaa tcaaggcgat tttcgcatct tgcagagagg tattttcaac
    13681 atcaatattt tgcgcaaaaa agaatgtggc aacaacagtg ataattacac ctatcagtaa
    13741 attcgattta tttgctttaa gcaatgtcca aatcgtttta taaagcgtca taacgcaccc
    13801 ctctttccat ccaagcattt cctaacgcaa aaaggaaaat aaatccgatc aaccatagaa
    13861 gattggtata aaaactagcc aaatcagtgt agaaataaag tttatacaaa ctttcgctga
    13921 tcaagttgac gagatttaat tgaccaacaa tcggaatatg aagatcaatc caatacttca
    13981 cttgttccga ccccatcata ccagcaaaaa agctcatcgc aatcgtcact gaaatcaaga
    14041 tgctatcctt ttgcaccaga ttcatttttg gtatcaaatt accaaccaat gtacctaaca
    14101 aaatcgcaca caacgacccc agagcggtaa caatcatcaa ccattgccaa cgttcaccaa
    14161 agtctacttg atagataaaa cgaaagacag ctaacataat ataaatctct gtcaaaacaa
    14221 tcgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
    14281 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
    14341 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
    14401 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
    14461 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
    14521 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
    14581 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
    14641 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
    14701 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
    14761 caattccagt attaatcgtt tcaagattgc caccttgatc aatctcgcca aaggccgcca
    14821 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
    14881 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
    14941 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
    15001 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
    15061 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
    15121 gctattgttc gctgaatcgc tgacaacaca ttttctggga gtaatgggat gtcgatcgtc
    15181 aaacgatccg ccgtccgtac catatctttt agagattctt tggttccttt agcaatcacc
    15241 tgtccttgat ccatgatcac gatctggtcg caaagaattt ctacttcttc catgtaatgt
    15301 gttgtataga caatggttgc tccttgtcga ttcagctcct tgatactttc taaaatttta
    15361 ttccgacttt gggggtctac agctactgtc ggctcatcta aaaaaattaa ccgcggcttg
    15421 tgggcaatcc cacatgcgat gttcaaccga cgtttcaagc caccactaag ttctcctgga
    15481 cgaaacgaaa catatttttc taatcccact aattgaatca cctcttcaat caaggctttc
    15541 cttttctctt tttcggaaac atataaaccg caaaaataat cgatattttc ttgcacactt
    15601 agttcgtcaa agaccgcaac gtcttgagga acgaccccaa tcattgcctt gacagcataa
    15661 ttttcaggag tcattgttgt cccgaacaat ttaatggttc ctttgtcata atttaacaaa
    15721 gaaagcatac agttgatggc agtcgatttc ccactgccgt ttggacctaa cagacctaag
    15781 acttctcctt cttgtacttg cagatcaaaa tgattcaatg ctacaaaatc tccatagcgt
    15841 ttgactaatt tatcaatttc gataatcatc atgaaaaact ccttccaaat ttcttgttga
    15901 tctgactata ataaagtgca agaaaagaaa ctagtgacga ttggctaaac cacacatgac
    15961 atttgtcaca atgaaggagt ctgctatgca taaatctcgg ctactcaacg aaactagctt
    16021 tctactggtt atctcattgc ttttgctggt aagtcaagga ttcgttgcta tgcaagttat
    16081 ctatttattg atcgccgttt tattcactgg tgtcagttat ttactgccta caaagccaaa
    16141 ttggattttc caagttattc tatttctttt atgtgccttt ttacctgatc ttgtcctgtt
    16201 tctgccagcg acgatgcgag tgatcgcccc tgaagaaagc caccttgatt acttgttttt
    16261 gttaggtttt ttgttgattt cgacggttgg taatcatccc cccatcaagg tcgcctttgt
    16321 cgtaattttg ctgttttttt ccttttactt acgaaaaaaa gatcaacgaa cgaccgatct
    16381 tgagaaaaag tacattgtct ccaaagatac tcattgggaa aaacaacaag agttatcgga
    16441 acaaaataaa gcattggccc tgtcacaaga aacacaatta gccttgcaaa ttgtggaaga
    16501 acggaaccga attgcccgag acatccatga caatgtcggt catctgctgt caagtgcgat
    16561 tttacaaatt ggtgcgcttg aaatgatcaa tcaagaacca aaactaaaag aaccattaga
    16621 aaatttaagt gccaccgttc acactgggat ggatcggatt cgtcaaagtg tccatgacct
    16681 ccatgagaca tcgatctctt ttcaacactc cttagagttt ctgatcgctg attttccttc
    16741 ttctgttacg attgaaggaa acttgtttga aggattgaat gaaacgcagc aaaactgctt
    16801 cttaatggtc atcaaagaag ccttaaccaa cagtatcaaa catagtcatg ccaaaaccgt
    16861 tgttttacat tttcgaacgt tgccggcctt ttatcgcttg caaatcacga atgatggcac
    16921 cagcccacaa aaaaattctg ctcccggtat tggtttgaaa acgatgcgtc aacgtgttca
    16981 agaacaaaag ggacaagtcc atattttcca agaaaaccag caatttcagt taacgatcat
    17041 tttaccaagg gagggacacc aatgataaca gtagtcatta ttgatgatga tccatttgtg
    17101 acgaaatcgt tacaaaccat tttagaaagc acgaaagaga tccgtgtgtt aggcattggt
    17161 cattgtgcca aggatgcgct agtcttatac gaaacccatc gtcccgatgt tttgctgacg
    17221 gacatccgca tgcctaaaca aacgggcatc gatgctgcca aagaaatttt ggctaaattt
    17281 cccaaagcca tccttttgct tttgacgaca ttcaaagatg aagaatacat ccgcgaagca
    17341 ttttccatcg gtgtcaaagg gtatttaatc aaacagaact tgcaagcgat tattccttct
    17401 gtcaaatccg cttataatgg acaggccgta tttggtaatg aaatcatcga gactttcact
    17461 caaatgatga agaataaacc gacgattgat agtcatgctt atgcttcttt tagtgaacgt
    17521 gagcttgcaa tcatcaaaga agtagctgcc gggaaaaaca ataaagaaat tgcagatgcc
    17581 ttgtacttga gtgacggcac tgttcgcaat tacatcagcc aattactaga aaagctagat
    17641 ttacgggatc gcacgcaatt ggctatctat ttttatcagc atttgcaata agtcttatct
    17701 aacacaaaaa aacctgaatg atgctccttc gcatcattca ggtttttttc acatcttcta
    17761 ttcaattgtc ccgcaaacca tttcctcacg attgaaagaa tcaaggattc gcagggctgc
    17821 caggattcgt tggattgttc ggcgtgttag gcggaaaaat aggcgccttg tctaacgttt
    17881 tttctagttt tgattcttca cgatcgtctc gttctacatc aggactttca ttcaatttga
    17941 attctttttc catctcatct aactttttaa agtcattgcc agattctttt gctaatttcg
    18001 ctaatttatc ttcggtttct ttagccataa agtaacgccc cccttttgat ttcagcttag
    18061 catatcacac tgaaaaaaca aaagaaaacg ctaaccaacg atcattttta gctatttctc
    18121 atctttaaaa tcaagcattt tttgccggta taaaatcgta gggagaactg ttttccatac
    18181 caatcatcgg atctactaac tggttatcga tcacttctgc tgtaagaacg aaatctttag
    18241 aaacagcagg ttctacctct tctttctttt cttctccaac aaatgaagaa aacagatctg
    18301 gttccactgt ttccattact tggtttttcg tttcattcgt cccactgtca tcttctacag
    18361 gtagcttttc ttttacaacc cgtggctctt ttgtatagtt cccatcttca aatggatcat
    18421 ttccgaccaa agaatcttca taagctgcta gtttggtccg gagagtgata ttcattttat
    18481 cattatccat gatccttgat ttcaacgtag tcatccggct tgctgattca ttcgctacac
    18541 aagtgacata ggcttgctct tcccctaaca aaatcaacag ttccttactg cgagtcactg
    18601 ccgtgtagag caaattacgc tgaagcatcc gctgatattg atggaccatg ggcaaaataa
    18661 ccatcttgaa ttcactgcct tgtgatttat ggattgagca acaataagaa agagtgattt
    18721 tattccattc gtttctttta taagtgacct catttgcatc aaactggata actaattcat
    18781 ccactttatc ttcagaatct tttgccaaga tgatacccgt gatttctccc atatctccat
    18841 tgaatacatt caattcggga gtattgacta attggaggac tttgtctcct atccgataaa
    18901 cggtgtcgtt ccaatgtact tccttttttc caggtttccc tggattgaat atctcctgca
    18961 tcattttatt taacgcatcg atccctgcag gtccccgata cattggtgct aatacttgga
    19021 tatcttgtgc acggaatccc ttatttttcg ctttttccac aattttagag atcaagggtt
    19081 caatttgata agcatcactt cggaaatagg aacgatcttt ttgattcttt gtgaaatctt
    19141 ttggcagacg tccttctttg atctcatggg caagaggaat aatactagaa ccatcacctt
    19201 gccgataaat ctgattgagt tccattttgg gaatttgttc gatttccaaa agatcatgga
    19261 gtacttgacc aggaccgaca gagggaagct gatctttgtc tcctactaag ataacttgca
    19321 tattcgtcgg aatcgctttg aataatgtgt tagccagcca tgtatcgacc atagacatct
    19381 catcaacaat caaaagtccg ccttctaatt ctttcgtact gactgaaggt gttttttccc
    19441 gtccattcag ccccagaaga cgatggatcg tactggcagg cagacctgtc gtttcgttca
    19501 ttcgtttagc tgctcggcca gttggtgctg caagtagaat aggaaaaatt tcttgggtat
    19561 attttgaagg atccaattcc agtccgttca actctgcaaa aagattgacg attccgttga
    19621 tcacagtagt tttccccgta cctggtcctc ctgttaaaat aaatagaggg gaacggattg
    19681 cttcagcgat agcttgttct tgcgaaggtc catactgtat gccgaatatt ttttcgatac
    19741 gccgaatatt cttctgcact tctttttcag gataatggat ttctttttta cgactgagaa
    19801 ggcgctggat cgaattaccg atcccccatt cagaaaagta caaactgttt tcgtaaagat
    19861 tagtctcttc ttgttgtatc tttccttctt caacaagttg gataattaca gtagcaacct
    19921 gatcaggctg gatttcaatt gggcgacttg tttctaaaat acggatcgtt tgctgaagca
    19981 atgtctccgc cggtacgtat gtatctcctg tttgaactgc atgctgaaaa atctgatgca
    20041 agatagctgc tcgaattctt ctcggtgaat ctgctgcaat accgatttgt tcggctagat
    20101 tatctgcacg tttgaacccg attccttcta tgtcttcgac tagctggtac ggattttctt
    20161 cgatgatatc caaagactcg ttcttatagg cctggtagat actgaaagct aactgactac
    20221 cgaacccgta acgattcagt cccatgatca cttgatccat cccgtggttt agccgaatcg
    20281 tctcagcaag catttctctt tttggcttcg tcaaaccgga gatttgtttt aataccgaag
    20341 gatcagcaat gattttttcg atagcatctt ctcccagcaa ttctacaatt ttttctgctg
    20401 ttttcttccc gattcctggg aatttttcgc tggaaagata cgctacgatc ccatttgcag
    20461 ttgttggctt ttcttgctca tatctttcga gtttaagctg ccttccgtat ttaggatgat
    20521 ccactaattc tccataaaaa cgataagtct cattttcttg aatatcgccg aaacttccgg
    20581 tgactacaat ctctttttca cggtaatcag ctgtggtttc actgatgtgt accaaaagca
    20641 ctttgtaaaa attactagga ttttgaaaaa aaatcgcggc aactgtacca acgaaataag
    20701 gcatttcttc tggctgcatc ggaagccgcc tccttcttct atagtaaagg ttctggtgct
    20761 gtttcagttc ctaaaaaatc tgtttggttc catactttca gttcatatgg caataatctg
    20821 caaggctctt cagtctctaa aatcgtcaaa ctactattga acaatccacc catttcacgc
    20881 aactcactta gctcttttcc tgtcatccat tgaatggctg cagttaaaga agctccatgt
    20941 cctacgaata aaagtgggcc atctttatgt cgggcaaccg catctgctac aacagtttcg
    21001 atacgtgtga ttgccgattc aatcgtttct ccgtgaaaag gagaaggatc atacagatct
    21061 aaatgattac gcaaattcgg cagattatcc ggatatttct ggtacatttc atcgatcgac
    21121 tgaccttcta aagtacccag ccctagttct cttagcttat ctgtataaaa aatctctacc
    21181 ggacgagcaa gctctttgtt gataccttct gctgtatcac gtgctcgctt tgctgtactg
    21241 gaataaattg ctttaaaagg aacatatttt acctttttcc caaatgcttt gatttcttcg
    21301 taactttgtg gcaaaagcgg agaatcgcca ttgctccctt gaaaacgtct ttcctgattc
    21361 cattgtgtct tcccatgtcg ggtaaaatat agttccatac gcagcaaact cctgtctttc
    21421 ttttatttta ctctataata aactgtctaa tccagcttgt cattttcata gtgatgcgtc
    21481 aattctaaat tagggaatcc ttgctggcga agtgcttcat acactatcat tgcagctgta
    21541 ttcgataaat tcaatgagcg gacatgctca tcattcatag ggatgcgtaa acacttttct
    21601 tcgttttcac gcataaatgt ttctggcaaa ccggttgttt cttttccaaa taaaaagaag
    21661 tgctcttctc catcagcaaa attttcgtca ctatatactt tatgggcaaa ttttgagatc
    21721 aaatgtagtt tacgattacc taaaaatgtt aaaaaagctt ctaaattact gtggtaagta
    21781 atatttacat cattccaata atccaaccct gcccgtttta gatgtttgtc atcagttgaa
    21841 aaacctagtg gctcgattaa atgaagcgga gtattcgtgg ctgcacacgt acgggcaata
    21901 tttccagtgt ttgcagggat taatggttca aataaaacga tatgattcat caggatcatt
    21961 cctctcatat ttatgttcgt ttctattttc tgacaatttg atttttgcat aaaaaaaaac
    22021 gtatcaactc ggtgtcagtc actgcgagtc gttacgttag tgaagctctt ctcactaact
    22081 tttatcagaa caggaacctg ataaaaacca atgaaaaaac aacttgttgc taatataaca
    22141 ccctcaactt ttttttgcaa cctctactca ttaaaaactg ctagaaaaac gttttctctt
    22201 agttcctttt aataattcta tgtcaattgt aatcttcttc aaaggattct tatataagtc
    22261 tgctttgatt cgctcttcta cctgccattc taaaggtgac atggccaaaa gatctttttg
    22321 attttcctct ggtatctcaa attcataggt tacacgctga cgttcagtta atttcattgc
    22381 ttcagcaaac ttggctacta cacgttcatt ggaataactc tgtttttcag gttgattcgg
    22441 ataaaaagcc tgacgtagtt cctttaaata attttcttgt ggcaccacct tcaacacttc
    22501 tccatcttct gagagtacac gttggaattc ttggtaatga gaaggcgaaa agatatttaa
    22561 aaccgcgttg aatgtttgat cagcaaacgg caaattggtc aaatcagcta cacaccaaaa
    22621 tgcttcggca gattgttctg tagctgcgta gactccttct tttgaaatat caaatccaac
    22681 agcattttga atctttttca attttgaaag ttcattcaag aagctacctt ctccgcagcc
    22741 aacatctaat agcgagactt cttcaggtaa atgctgcgca atagtctcaa taacgggctg
    22801 atacatgcca gattggatca ttctacgcct tggaacgaac attccttgat cgtattctgt
    22861 tttaaccgca tgggataaaa aataaagtgt tccttttttg gaaaggtcaa aacggtgatt
    22921 atttttgcat atcaagccat tatcagactt ttttacttct tcttggcaaa ccggacagcg
    22981 gaacagctga tgattttctg ccaaaaactg gcgtcctcgg tcaatttttt ttaacatagt
    23041 tccccctcat ttctgtttta tcttaccgtt agatgctcac tttggcaact gctaaaaata
    23101 tgatacaatg cctagaagaa agtgaaagat aaaggagaag attagatgat caaagaattt
    23161 tgcgctgaaa actacacatc tattcctcta gcaattgcta gaggagccga tcggatcgaa
    23221 ttgtgtgaca acttagcggt cggtggcaca accccgagta ctggtgtgat cgaagaaacg
    23281 ttaagttatg ctggggaaaa agatatcccc gtaatgacga ttattcgtcc tagagggggc
    23341 gacttcgtct ataacgatat cgaattgaaa atcatgcata ctgatttgat tgaagcaaag
    23401 aaattaggga cggatggcgt agtatttggt tgtcttactc cttcaggctg gctagacgaa
    23461 gaggccttag aactattgat cgaaacagca gaagggttgc agatcacctt ccatatggct
    23521 tttgacagta ttcctgaaga tcggcaattc gaagcaatcg attggctggt agaacatggt
    23581 gttgaccgta tcttgactca tggcggtcct gctgacaaaa ctattgtaga aaatttccca
    23641 cggcttaaag aattgattgc ctatacagat ggccgcttaa tcattttgcc tggtggtggt
    23701 gtcactgcag agaatgcagc acatgtagct aaagaattga acgtaagcga agtacacgga
    23761 accaaaatcg tggatcttca gttataatct tataaacaaa aaaacatttg caacaggcat
    23821 aatgcttatt gcaaatgttt ttgttttttt acataaaata gcccataaat aaagaagcaa
    23881 tattgaaata aatcagtaca ctcgtcaaat cgctcaaagt cgtgataaag ggaccgcttg
    23941 ctactgctgg atcaaatcct agacgatcca ttagcatcgg gattaaacta cctgcaagat
    24001 tcgccactgt gattgcaaat agcatagcca tccctatgac aaaccccaag ataaaattat
    24061 gtttccaaaa acctacgatt agaaaaatcg tgatccccgt aacagctcct gtgacaacgc
    24121 cggtcaagac ttcagccaaa acaagtcgaa caaatccatg atctttctca tcagaaatcg
    24181 caagtcgacg aacagccaca gctagcgact gggttccagc attaccggca gttcctgtaa
    24241 tcagcgaaat gaagactgct aaaatactcg cttcacttac caagtcttca tagtgactga
    24301 ttaaagaagc ggtcgacata cctaaaaaca acagagtgat cagccaaggc aaacgtcgag
    24361 acgctgcctt tactggattt tcattaattt cttccacatt gaccccggca agtcctgaat
    24421 aatcgctagc agcttcatca tcaataacgt cgatgatatc atcgactgtg acgattccta
    24481 acaaatgatc atcataatcg gtaacaggaa gagctaagaa gtcataatct ctaaatgttt
    24541 gtgccacatc ttcttggtcg tcgcctacat gaactgaaag cactcgttcg ctcatcagat
    24601 cagaaatcat tgcgtcgtca tcattcacga ttaggtcgcg cagagaaata acccctacta
    24661 gctgaccttc ttgattaacg acatagatgt aatagattgt ttcagctaca tctgcttcgt
    24721 tcttcagcac gtacatcgca gagcgaacag tttgattggc tacgatcgaa acaaattcag
    24781 ttgtcatgat cgcgccagcc gtttcatcct cgtaatggag aagttccttg atttcactgg
    24841 catcgtctgc cgacatcaaa cttaaatatt tagcaatttg ctttttatcc agcgtattaa
    24901 gcaagtcaac agcattatct gtatacatct cagaaagcat atcagctgca tagctaggac
    24961 gcatttcatc taggtattct ttcatatgtt cgttgtcttc ttcaatcaca tcgaacatat
    25021 ccgctaattc tttaggcgac agataggaat agatcagctg cctgttctct tctgtaagag
    25081 attggtagaa ttgcccctgc tcatagatat gcatctctaa aaaactgttt ctgaatgcct
    25141 gaatattatt ttcccttaaa tcttgagtca actgggcaaa tctttcctct aattcttgat
    25201 tttcatccaa accaatctca cctcgcctta ttcttcaaac cgtctcacaa ctgcctccat
    25261 gtctgcggct aaaggctgct tgaactccag caactcttct gtaaatggat ggtaaaaacg
    25321 cagatcataa caatgaaggg cttgtctttt tattcccaga tccattcgcc cgccatacat
    25381 atcatcaccg agcagggcac agccaattgc ggaaaaatga actcttattt gatgtgtcct
    25441 tcctgtatgc agctggatat caactaacgc gatttctgtt agtcgcttct ctatccagta
    25501 ctcagtctgt gccgaacgtc cttcatcaga gaccattcgc ttcaatagcg aagaataatc
    25561 tcgggcgatc ggcaaatcga tatgtccatg tgccgataaa tctgaaagcc ttccactgac
    25621 taaggcctga tattttttca caaacttctt ttcacgaagt tgcatatcta attttgcgtg
    25681 cgcaaatcca tgctttgcaa aaagcatcaa gccagatgta tcccgatcta gtcgcgtcac
    25741 cacatggatt acttggtttt cgtaaccttg ttttttataa taagccttta cacgattagc
    25801 cattgtccca tttggatggt attgggcagg aatagacgaa acgcctgctg gtttatttac
    25861 taccagaacg tgttcatctt catacacgat atcgatcggt gtttcatcca aaagtacggt
    25921 ttcatgttct ccttcagcag gaatcacaat ggttacctta tcatccttag ctagagaaaa
    25981 caagacattt tctacttgat cattaacttt gatttgaccg ccttgaaatt ttattttcgc
    26041 taacagaccc tttgagatcc ctttttcttt taaaaaatat tttacttgct gagaatgatc
    26101 tttctcataa tcccatgtaa actgcatatc aatcctcact aataaaggca tctttcacac
    26161 gatgccaaaa atgcatatgt cgataagaag caaaatggat ccgttcttct gctattcgat
    26221 aataaattga gcggatattt gcttttgcga cattcaattg atcaaccgtt acaagataat
    26281 cttctgtatt ttccaatttt atttctagcc attcatcatg ggcaatcacg atcggagaac
    26341 ccaaagtacg aaacactcga ttattcagtg aagcaatctc tgccaactga aaggcattga
    26401 tactaggatg gatcacagca ccgccgacac ttttattata tgctgtcgaa ccagtaggcg
    26461 tagaaatcgt caatccatct cctctaaaac tttcaaacag ctcgtttttg atgtatacat
    26521 cggctaccat cgtacgattg gcccgtttaa tcgttgattc attcagtgct aaaaaatgct
    26581 ggtccggtgt ttcatccaaa taactgatcc gaacatccag caagggataa ctaacacttt
    26641 gttcccggtt cgttcgtaag ctgtctacta gttcttccag ctcgtaatcc cgccaatccg
    26701 tgtaaaaccc taaatgacct gtatgcacgc ccaagaaacg cacatcattc agacaatgac
    26761 tgtagtgatg gaacgccgac aataatgtgc cgtctccgcc gactgagatg accaattctg
    26821 gctcatgatc atctcgttga atccctgctt gttctaaaag tgcgttcaaa cgttctgtca
    26881 cttctatagt ctttgcttct ttattatgaa cgatcgctac tttcatcccc tgcctcctct
    26941 ctttcgcttt ctttcttcaa aagcgttcga tagtagacat catctgattt tcctcttcca
    27001 tgggagaata accgctgtgc ttcttggatt tcttcacgga tactggacat ctcttcatcc
    27061 aactgatagg cagcttctgc cgcacgctga agtcgttcac tcatttcttc tggaaaaacg
    27121 ccttgatatt tataattcag cgaatgctca atcgttgccc agaaattcat cgcaagagtc
    27181 cgaatctgga tttccgccaa aattttcttc tcaccgctga tcagctgtac aggatactcg
    27241 atcaccagat gataagaacg gtagccactt gcttttttat ttgtgatgta atctcgttct
    27301 tggagaactt tcatgtcttt tcttttacgg atgatctcaa caacttgatg gatatcttct
    27361 acaaactgac acatgatcct taacccggca atatcagaca tttcttcttc taaacgtccc
    27421 attggaatat gccgtaattg tgattttgtt acaatactat cgactggctt gactctccca
    27481 gtgacaaatt caatcgggac atgtcgattt tgttcacgga actgtttgcg gatcccccgc
    27541 agtttcactt tcaattctga tacagtttgt tcatatggtg ctaaaaatgc ttcccaatct
    27601 cgttccatag ctgatacctc tacttcatta acattcattg tttattctac catatatgca
    27661 aagttttttc gtactcttga tttatgcatt gaccaaaaaa agaaaattag gcaaaataga
    27721 caaaaaggag tcgatcaaat gacacaaatg cttgaaatcg aatataaatc tatgctgaca
    27781 aagcaagagt acgagatatt catcgctcac tatcaattaa cagataaaga tttccgtgta
    27841 cagactaata tttatttcga taccgttgat gaacagctga agaaacaaaa ttgtggccta
    27901 cggatccgtt tcgttggtca tcaagctgaa tatacattaa aaacacctgc tgaaaaaggg
    27961 cgtctcgaaa caacggatac ctttataaca gaagaagcag aagcatttat cttcgaaaaa
    28021 cgtctgcctc gttcaggcgc agtttttcaa aaactttctg atttgaatat cgatcctgct
    28081 tcattaatcc aaaccggaaa gcttacaaca aaacgagctg aattccctat tgaagaagga
    28141 cttttagcta ttgatgaaag ctggggagac aatctacacg attttgaact ggaactagag
    28201 gtaggggatg catctgtggg aaaaattgct tttataaatt ttttaaaacg tttttcttta
    28261 ccttatcgtc ctgctaagaa caagatccaa cgtatgcttg aagctaaaaa ttagaaacct
    28321 ttcattttca caaggcactt gagttcattt ttttactttt ttctgttaag ttataggatg
    28381 tgaaagaatc tagtcactag ttgtatagaa tctattttgg gggagattca tatgatcgaa
    28441 atctatttat ttgttaatcc tttaggggaa tactgtttcg aaatggaaca acagttatta
    28501 caatttatcg aagaagagta cgggacacca tcaaaagaaa agatgcaatt tcgctttctt
    28561 cctttggtca atttgcagac aattggggat gtcatgcaac aaaaaggaat ttctcagaac
    28621 aatcttgagg aacgtaatca cttattctca atgacttatt ctgcagcatt ggactgcaaa
    28681 gcggcgcagt ttcaaggaaa gaaaaaaggc cgccgttttt tgatcaaatt gcaagaagca
    28741 gtcggctgta atgacatacc atattctcaa gaacttgctg aatccatatt tgaagaaatc
    28801 ggcggcgatt tgaatatgtt taaagaagat cgtcagtctg acttcgttaa agaagtattc
    28861 ttttctgatc aaaaaattgc tcgggaaatg ggcatcacaa aacacccttc tgcagtcgtt
    28921 tacaattatg cctgcgaccg tgattttggt gttttagtag aagtaaaagg tacaagtagc
    28981 ctttggagaa tcacagagct gtgtaaaaca acagataact catatcaagt ttttaatggt
    29041 gaacatttac atacaaatga ttctcagttc cattcaagaa ctagccattt acacttgctt
    29101 tctaattaaa aa
//
LOCUS       DACCCS010000035        28598 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.035, whole genome shotgun sequence.
ACCESSION   DACCCS010000035 DACCCS010000000
VERSION     DACCCS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.035"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            236..1876
                     /locus_tag="IVK82_001610"
     CDS             236..1876
                     /locus_tag="IVK82_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP6392678.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2030..3352
                     /locus_tag="IVK82_001611"
     CDS             2030..3352
                     /locus_tag="IVK82_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP6392679.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3414..3845)
                     /locus_tag="IVK82_001612"
     CDS             complement(3414..3845)
                     /locus_tag="IVK82_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6392680.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3909..4745)
                     /locus_tag="IVK82_001613"
     CDS             complement(3909..4745)
                     /locus_tag="IVK82_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP6392681.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4935..5237)
                     /locus_tag="IVK82_001614"
     CDS             complement(4935..5237)
                     /locus_tag="IVK82_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP6392682.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5263..5694)
                     /gene="ruvX"
                     /locus_tag="IVK82_001615"
     CDS             complement(5263..5694)
                     /gene="ruvX"
                     /locus_tag="IVK82_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP6392683.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5691..5957)
                     /locus_tag="IVK82_001616"
     CDS             complement(5691..5957)
                     /locus_tag="IVK82_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP6392684.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6211..6936)
                     /locus_tag="IVK82_001617"
     CDS             complement(6211..6936)
                     /locus_tag="IVK82_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP6392685.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7302..7372)
                     /locus_tag="IVK82_001618"
     tRNA            complement(7302..7372)
                     /locus_tag="IVK82_001618"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7338..7340),aa:Gly,seq:ccc)
     gene            7489..8241
                     /locus_tag="IVK82_001619"
     CDS             7489..8241
                     /locus_tag="IVK82_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP6392686.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8257..8736
                     /locus_tag="IVK82_001620"
     CDS             8257..8736
                     /locus_tag="IVK82_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP6392687.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8987..10567)
                     /locus_tag="IVK82_001621"
     CDS             complement(8987..10567)
                     /locus_tag="IVK82_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP6392688.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10560..11303)
                     /locus_tag="IVK82_001622"
     CDS             complement(10560..11303)
                     /locus_tag="IVK82_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6392689.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11479..12255)
                     /locus_tag="IVK82_001623"
     CDS             complement(11479..12255)
                     /locus_tag="IVK82_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6392690.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12503..13339)
                     /locus_tag="IVK82_001624"
     CDS             complement(12503..13339)
                     /locus_tag="IVK82_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP6392691.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13350..14279)
                     /locus_tag="IVK82_001625"
     CDS             complement(13350..14279)
                     /locus_tag="IVK82_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP6392692.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14471..14824
                     /locus_tag="IVK82_001626"
     CDS             14471..14824
                     /locus_tag="IVK82_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP6392693.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14898..15554)
                     /locus_tag="IVK82_001627"
     CDS             complement(14898..15554)
                     /locus_tag="IVK82_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP6392694.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15780..17714
                     /locus_tag="IVK82_001628"
     CDS             15780..17714
                     /locus_tag="IVK82_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6392695.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KTRVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17800..19023)
                     /locus_tag="IVK82_001629"
     CDS             complement(17800..19023)
                     /locus_tag="IVK82_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP6392696.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19017..20915)
                     /locus_tag="IVK82_001630"
     CDS             complement(19017..20915)
                     /locus_tag="IVK82_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP6392697.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20909..22027)
                     /locus_tag="IVK82_001631"
     CDS             complement(20909..22027)
                     /locus_tag="IVK82_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP6392698.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22110..22943)
                     /locus_tag="IVK82_001632"
     CDS             complement(22110..22943)
                     /locus_tag="IVK82_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6392699.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22936..23853)
                     /locus_tag="IVK82_001633"
     CDS             complement(22936..23853)
                     /locus_tag="IVK82_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6392700.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24080..25204)
                     /locus_tag="IVK82_001634"
     CDS             complement(24080..25204)
                     /locus_tag="IVK82_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6392701.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25387..26295
                     /locus_tag="IVK82_001635"
     CDS             25387..26295
                     /locus_tag="IVK82_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6392702.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26436..28049
                     /locus_tag="IVK82_001636"
     CDS             26436..28049
                     /locus_tag="IVK82_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP6392703.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8890 a         5621 c         4867 g         9220 t
ORIGIN      
        1 tctatttatc tatctaacat atcctttatt cttctacttt tctttcttta gcatataaga
       61 gatacgcata gtaatcaagc aaggattgtt gatataaaaa gaacaaatac cgctattttg
      121 atttcttttg actctattac tcatataaat aaaatttatt atacctgctc tttttgacta
      181 tatcctcatc tgactcctat acttcgtttg tattaaaact taggaggaat cattcatgct
      241 tacagggcta aacttattaa ataatccttt tctaaataaa ggaacagctt ttacaaaaga
      301 agaacgggca aaatacggaa tcactggtat gcttccaagt actgtccaaa cattggaaca
      361 acaatctgtc caagcttacg gtcaatactt aagcaaacag accgatttgg aaaaacgtat
      421 cttcttaatg aatctattca atacgaaccg gacattgttt tataaattga tgggcgaaca
      481 tttagttgaa ttcatgcctg tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata
      541 caacgaaatt ttcttgaaac cacaagatgc tgtatttctt tcaatcgacg aaccagaaaa
      601 tatcaaagca agtttgaaaa acgcagcaga tggacgcgac attcgtttga tcgttgtgac
      661 agatgcagaa ggaatcttag gtatgggtga ctggggtgtg aacggtgttg acatcgctat
      721 cggtaaattg atggtctaca ctgccgcggc tggtatcaat cctgcacaag tcttgcctgt
      781 atcaatcgac gctggtacta ataacgaaga attattgaat aatccattat acttagggaa
      841 tcgtcatgct cgtgtagaag gcgaaactta ctacgaattc attgatcaat tcgttcaatc
      901 agctactgaa ttattcccag aattgttatt acattgggaa gattttggac gaggaaatgc
      961 tgcaacgatc ttagaaaaat acgaagataa aatcactact ttcaatgatg atatccaagg
     1021 cacaggaatc gttgtactag ctggtgtatt aggtggattg aatatatcag gtgagcaatt
     1081 gaaagatcaa acgatcttga catttggtgc tggtactgct ggtgtaggta tcgcaaacat
     1141 ccttttagat gaaatgatcc gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta
     1201 tcaagtagat aaacagggtt tattgtttga agatacaaaa ggattgactc ctggtcaaat
     1261 cccattcgct cgtaaacgct cagaattcac taatgctgat gaattgacaa acttagaagc
     1321 agttgtaaaa gcaatccatc caacgatcat gatcggtact tctacccaac ctggagcatt
     1381 cactgaagaa atcgtgaaag aaatggcggc tcatacccca cgtccaatca tcatgccttt
     1441 gtctaatcca acaaaattgg cagaagcaaa agcaaaagat ttgattgaat ggacggacgg
     1501 aaaagcttta gttggaacag gtatccctgc tgctgatgtg gaatataacg gtgtaactta
     1561 ccaaatcggt caagcaaata atgcgttgat gtacccaggt ttagggcttg gtttgattgc
     1621 ttcaacagct acacgagtaa atgctgaaat catttctcaa gctagtcgag cattaggcgg
     1681 aatcgttgat gtaacaaaac caggagcagc tatcttgcct ccagtagcaa aaatcactga
     1741 attctctcaa acgattgctg aaactgtcgc aaaatcagtt gttgcacaaa aattgaatcg
     1801 tgaagaaatc acggatatca aagaagctgt tgaatctgct aaatgggttc ctgaatataa
     1861 atctttagaa gattaagatt gttatagtaa aagaacatac agaaactttg tatgacgtct
     1921 aaaaagaaaa aagcgactgc ggcaaaacag cagccagcca actactgctc ttttgccgct
     1981 tctctttggc ttttttcata cgatacagaa taagaaagga tctgtaaaaa tggcacaaaa
     2041 aacaacaacc gcaactgcag ttgaggaaaa gaaaaaagaa gggttttggt caacaaaaat
     2101 taccggcgta agtttgcccc tttatttgat catggtcctt gtcctgatca ttgcaatggc
     2161 tttaggaaaa ttaccgacga atatgattgg cccaatcgct gttttggtca ttttcggtaa
     2221 tttatttcat tttatcggaa caaaaatccc tatagtcaaa agttatttag gtggtggctc
     2281 cgtttttgcg atctttgcct ctgccgcgat tgctacattt gggatcttac ctgagtatgt
     2341 agtaaaatcc acagaaaact ttgtcagcaa tatgggcttt ttagactttt atattgctgc
     2401 cttgattact ggtagcatcc ttggtatgaa ccgtaatcta ttgatgaaag cttctgttcg
     2461 ttttattccc gttgcactta tttcaatggt cgtctccttt tttgccgtag gactcgtagg
     2521 tatgcttatc ggaaatggtt ttgcaaattc cgttttatat atctcattgc ccgcaatggc
     2581 tggtggtgtt ggagccggag ctgtaccttt atcaacgatt tatgcaaatg ttttaggtac
     2641 ggacgcttct tctatcattt cccgcctgat tcctgcgaca gcaatgacaa atgtcctagc
     2701 tatcattggt gcagcgttag ttgctcgtgc gggacaatca atgccaaaat tcaacggtaa
     2761 tggtaaatta atggaaaaag gcgaccttgg cgatggaaaa cctagaaatg taaaaccttc
     2821 cattccacaa ttaggtgtcg gtttgatggt atcattgact ttcttcatct taggacaaat
     2881 ctgtaactat tttgttccta aagtccatgc ttacgcattc atgatcatta tcgttgttat
     2941 ctgtaaaatc gcaaacctat taccagaata ttacgaagat gcagctatca tgttcaacaa
     3001 cttgattgtc aaaaacttga cagcagctgt tcttgccggt atcggtattg cgttattgaa
     3061 tctaaatgta ttggcttctg ctttgacatg gcaatttgtt gtcttatgtt tgacaagtgt
     3121 tatcgcgatt tcagtagttt caggatttgt cggtcgtttg tttggtttat atccaatcga
     3181 atcaactatt actgctggct tatgcaacaa ctcgatgggt ggtacaggaa atgtagctgt
     3241 cttatctgca gctaatcgga tggaacttat tgcttttgct caaatgggca atcggcttgg
     3301 tggtgccatc gttttgatca tctcaggttt cttgatgcaa ttactttctt agtcaaaaaa
     3361 aatacctgat catcaagtat cggcttgatg accaggtatt ttttgataaa aatttacagg
     3421 attttcaaaa atcttttttg cattttatcg gaatgataac cgatgcgttc ataaaatcgg
     3481 tgtgcttcta ctctgtattc tgcagaatta agtcttattg ctgatattcc tcgtcttttc
     3541 gcttcttcta cgccagtcat caaggccttt ccgattcctc ttttttcagc atgtttagac
     3601 acagcaagtg ccataatatt gaacatcgga tcgctgaaaa tcgattcata ggactccgct
     3661 tgtacatagc cagctgcttt tttagaagta ggatcttcat agaccaagaa aaaatttttt
     3721 tctggctcct tcaacagttt cttcatttgt ctcaccgtaa cggttaatgg tacatcatat
     3781 cctaattgtt ccgcattgat ttctcgtagt gcttctgcat ctcctaaacg catttcccgc
     3841 aacatccaaa tcaccagcca atttttattt ttttaaatga atttttttat cacagtatac
     3901 tccttttttc acttaaaaaa agacttttca gtgattttga cttgcttgaa tccagatcct
     3961 tctaatgttt gcaagatgac gaaagcattg taaagattga tcaaaagaac cacgatgaca
     4021 atcgcttgaa aaataaaggt cacttttttt ggagacaaaa ccctgctaaa tcttgctccc
     4081 cacaaacctc caataacagc agcggggata acataaaaca acatagataa atcaaagcgc
     4141 ataaagctac ttgttaaagc aatcgttacc aattttgcca gttgagaaaa gaaaatcgta
     4201 gatagagaat atagtgttgc ttcttttatt ggcaatgcaa acatcagcat caacaaagaa
     4261 acattgactg gtccaccacc aatacctaaa aaactagcta aaaagcccag aaccccccca
     4321 catataaggt accaagtcca tgacgtcaaa tgaaattttg gctgatgatg cttggtgtag
     4381 aagaacgcaa aaaccaaggt caataccgtc aaaaaaattt ggatcagctg cacatgcttc
     4441 tcgttttcaa ataactgtaa aaaaacttcg aatacaatgt tgccgactac tcctccaaca
     4501 acagctccac tagataccca accgacaatc tgccaattca gtgatttccc gcttgatact
     4561 tgtcgcacag tcgaaacaat ggacatcgtg aataccgcaa ctgttgagta aaaagaaatc
     4621 cctgcaacag aatgggcgcc gataagatca agaatcggtt tgatcaacac gcctccaccc
     4681 atcccggaaa tagcgccaac tgtgttcgcc agaacgatca ccacaaaata aatcagacca
     4741 atcatcttgt atttcctttc tcttgtttat atcttttagc gattgttact cttttctttt
     4801 attatacagt aaaagaaaca aacgaggctt cgaaagcttt tctacttttt aaggaaattt
     4861 agtgctaaac gaaaaaaccg ggctctactt ttcagagagt ccagcttttc ttacacctta
     4921 tcgattcttt tgttctattc ttcttccgcc atgaatgtat tgaatacttc ttcgatcata
     4981 tcccattctg cgtctgtttc gatttgttct aactcgcctt ctgttccatc ttcgttttcg
     5041 atgtatgcat aagcttgtaa ttctacatct tcatcttctg aaacaccagc tggatataaa
     5101 agaacatagt tacgtccaaa ttcttcttgt ccgtcaactg ttagcaagat ttcgtataat
     5161 gtttcatttc cttggtcatc caccaatgtg atgtgttcgt gtccttcgtg ttcgtgggaa
     5221 tggttatgtt ctgtcataag attacctctt ttctttttat tattaaaata attgcttacc
     5281 tgagccgtct aaataatttt gtaaaatcat cacagctgct aatttatcga tgactttttt
     5341 tcgtttcgct cttgaagtat ttgcctgctc tactagcata cgttctgcct gaacagtggt
     5401 tagtcgttca tcttgataat caaccggcaa ggagaatagt tctttgatct tctctccata
     5461 tgacatggaa gcttctgccc ttggtccgat ggaattgttc atattcttag gtaatccaac
     5521 tacaaaacgg tctacttcgt actgcgccac taattccttc aaccgatcaa aaccaaactc
     5581 ttcttctgcc tcattgattc gaatgatttc tactccttga gctgtccagc caaagggatc
     5641 gctcacagca acgcctactg tcttagaacc gacatccaag cccattactc tcataaattc
     5701 actccatgat ttgagaggta gtctttgaca attacttcca tgatttcatc gcgttcgtga
     5761 cggcgaatca aatttcgtgc atcacgataa cgaggaatgt aggccgggtc tccagaaagc
     5821 aagtagccga caatctgatt gattggattg tagccttttt cttccaaagc ccgataaaca
     5881 atcgctaatg tttcgctcac ttcttttttg cgactatcgt caaaaccaaa acgtacggtt
     5941 tcatctgtaa aacccatgat tctacacctc tttcccgcaa ggtaattcca gcttaccttc
     6001 tacaccctta ttttactcga agaaagggaa aactacaaac aaatattcca aacttctgaa
     6061 taaattaaga aacacgattt gtctgatttt tccttttttc tgtggctttt tcaaaagccc
     6121 tctttccata aagtatacgc tatatttctt tgaaagtatg tttttacaca attttttcat
     6181 aaagttcata aaaaattcga attgctgcta ctaatttttc gttttcgttg cttgccctgt
     6241 ctcataattg atggtgtagc catcttgaac attgtagata aagacattga attcaatttt
     6301 tttgtcttca atgctttctg cttccaactg cacacctctt gctaccagtt catttccctc
     6361 gaaatgagga gtgacacgat agcgtacatg atgatttgtt tccttgatat atgccgcaat
     6421 atcattttcg tgggcaagca tgtacggagc attcaacgaa cgtgtccctg tcattaagtt
     6481 tttaatgtta ttattttgac cagtcaactg gaaaccaatc aaatggctgc ggttgtataa
     6541 ccattgtccg tcacttaatt tcttttgttt ccaacctgtc ggatcaatat acaaaggttc
     6601 tctcttttca gtaggaaaca agtcttttcc caacatcgca ttggcttgtc ctactctgtt
     6661 caatttgtct atatttgaat agctttccca tgtccctttg gaaagactaa gatcctcttt
     6721 tgtaaactcc ggctgattcc cattgatttc tatttcttga tgttttccat catattctgg
     6781 cacatccgct acttgagaag cttgcggtgt ggaatcaaaa atggttgtaa tttgttggaa
     6841 gatatctgaa tctttttgtg tggtggaaat accgagtgcc cctgcaatca cagcagcgac
     6901 gatccccacg atcgccttgg ttgttttttt gctcatttct ttactccttt actaacagtt
     6961 ccctaaatct taacatatgt tcccctataa ataaatactg gaaagaaata gaaacagtaa
     7021 aaaaataaaa ccagttttta gacggcttaa gaatacaaaa aaagagacgg attcatcgtc
     7081 tctactcaat cacaaaacac tatttacaag gtatttatta tcattcaaaa aatttttctt
     7141 aatactcaaa actaaacggc ttctgtacga cctcttggta aacagtgttc aaaagcttgc
     7201 atctgcggta ataagtccaa ccaagtcaaa aacataagaa gcttttttgc ttgactattc
     7261 ttattacttg atgcagggca gctttttcac agcctctttt aagcgggtga cgggaatcga
     7321 acccgcgaca tcagcttggg aagctgggat tttaccacta aactacaccc gcgatacaaa
     7381 aacagtatac gcctctttca ctccttcctc aagttttttt attctttacg cttccgatat
     7441 gttttgtagt aaaataaaac taatcaatca attattggag aagatgctat gacgggaaaa
     7501 gaattagatc tgttgctgga cacttgttta ctcgctggga aaatcatgat ggaaagcaat
     7561 gcggagatgt atcgggtaga agatacgatg agtcgaattg ctttagcttc tggaaatttc
     7621 cgtttggtca gctatgtcac tcaaaccggt ctctttattg gtcttgatcg tacctcgacc
     7681 atccgaatgg aacaaattac caaccgttcg attaatttag aaaaagtagt aaaagtaaat
     7741 gatctttcac gtaaatatgt tgcttcagag ctaacattag aagaacttta ctgcgaactg
     7801 caagcaatcg accgagaccg ttctttcttt cctgtctggt tgcaaatcgt tagcgcggca
     7861 gtgatcagtg gaacaattat ggtgttattt ggcggaaaga tgaccgattt acctgtgact
     7921 ttagtcattg gaggattggg atacgcagct tatctatata gtttgaaatt ttttagaatc
     7981 aaatttttat cggaattttt atcttcttta ttgattggca tagccgctat ctacagcgta
     8041 cgttttggct taggaagcaa tcaggatcta atcattatcg gatgtgtgat gcctttagtt
     8101 cctggagttc aaattacgaa cgctatccgt gatttgctag ctggacatta tgtctcagga
     8161 gtttctcgcg gaacagaagc gatgatgact gctacgatga tcggttttgc gattgctttt
     8221 attttccagc ttttttatta ggaaaggaac acaagtatgg ggacgatatt tattcagttt
     8281 tcttttagtt ttttagcgac tgcggcattt gccgttatta caaatgtacc taggcgttca
     8341 ttgatttgtt gtggtctttc tggtgctttt ggctggatga tctactggat ctgtgttcaa
     8401 ataggcggat ctgctgcttt tggttcattg cttggttctt taggagtagc attcatcagc
     8461 gatcttttct ctaaacgttt gaaaatgccc gtaacgattt tcaatattcc cgggatggtt
     8521 cctctagtcc ctggtggttt agcttatcaa gcggttcgaa atctcgttac tgaatcctat
     8581 cgagaagcgg ctcattatgc ggtccaagca attatgattg ctggagcaat cgctttaggt
     8641 cttgtattat cagaagtctt taaccataac atccgtaatt tcaaagtaaa gcgtgacgca
     8701 atcatgatga aacgaaaaaa acggcttttt aaataaacga agtgtaacaa agaaaaggtt
     8761 gtgaaagaga cgttcagcct tgagcgatga caaaatatct cacaaacatt gcgacaagta
     8821 accgcaggag caataagaag taattatgag tattaaaaaa agttttgtaa aaatagcttc
     8881 tcacattttt acataaaatt gacttaatac tgagaaactg acttttgaac accctctatc
     8941 tgtaaaaaag gggctgcgac atgatttttg tcacggcccc tcttctttat tcaaattttt
     9001 tccaaaacgt tcgttgataa taaacgatcc atagagcgga aagtattaga actaaaccga
     9061 tcacaggaat attcactaac caaaatggat aaaacaaagc caacattccg tataagacga
     9121 taagaataat actgatgatc aatgaaccca tcatagttgc gactaaaccc aaacttcctg
     9181 aacctctgtt aaataattgg ttaatattgg tccagttcag cagcagcaac cgataatctc
     9241 tagcgaaata acgtagtgac aataagtagc agccgaataa agaaccaaat agcaagctag
     9301 ggataaaaaa caaaggcagc tgaaaagaca gtccgcttaa taatgcgatc ccaccagtta
     9361 aaaaactctg caatatccat ccgaatctga acttttcttt taaatattgg ttcatagaga
     9421 ttggcaaaga acggataaac agaaaatttt cttgatccaa agaaataaga ttgctgataa
     9481 aagaagtctg attaactgtt aagaaagcca atgcgatccc taccaggaaa acaacaccga
     9541 ttaacttatt atctaaatca gttaatttta tttcgcctcc tagggcaaaa gcaagaataa
     9601 aaatgattgg agttaacaag gaagaagaaa gaacttgcat aatcaaatta ggttctttta
     9661 ctaattgagc attgtaacta aataaaagct gatgcacatt ttggttcttt ttatgggttc
     9721 tttttatttt gaactcactg tttgaatcag tcagttgctc tgtaagcttt ggtactatca
     9781 gctgttttac agcaaggaaa aatcccagag ccgtgatagc tagtattccc caacccaata
     9841 aaccttgttg agaaagtggc tgactggcag catcatagaa tggcaataat atcgaaatcg
     9901 gatgccgatc tgttaactca ttttcaatag tccccgtttg atgattcatc cacatgattc
     9961 cagcaaatgc gatcagcatc gagctgccaa gcaagatact agaaactgtt tttctatgtt
    10021 ttttaaacac tgctgttcga gccaaaccaa aaacaatcaa actagccaga caaaaaagaa
    10081 taatcaaaaa tagagaaaac aaaaggattg agataatgat agtaagcaaa atccctagat
    10141 cagctcgcca tccggttaaa ataaacatga ctagaagcgg aaaaacaaaa ggaatgatgg
    10201 tcaaagcaac taccaaaatc tttgctgaaa aaacctgttc ttgtccaaaa ggcagaggca
    10261 aaaatacttg caggtcattt ccttcaaaaa agatattata aatgactgat atactttgag
    10321 ttaaacctaa cataccaaaa agtgccacgt aataagtaaa gaaacctggc aatttaacaa
    10381 agttcatagc aaacatagca aatccgtaaa tcagtaaaaa aatcagccct gaaaaaagat
    10441 tttgagacaa aagatagata gttaatcgct ttcccttttt tcctttctct cttgctttat
    10501 tcgtgagctg tggattaaca tataaaagat taactcgaat taattctaat aattgtcgct
    10561 tatacatacg gtccatctcc tgtcgcttgc cttccagcca ttttcaaata aatcttttct
    10621 agcgtttcat caggtgagcc attcaataat tcactaactg ccccctgata aatcaattca
    10681 cctttcttca aaatagccag tttatcgcat aactgttgtg cggtatccaa tacatgtgta
    10741 gaaaaaatga ctgtttttcc ttttgcggca tgagccttca tcatttcttt caaatcaaat
    10801 gctgcttgag gatcaagtcc ttgcaaaggt tcatccaata cccaaatatc tggatctggc
    10861 agcaacgtgc cgatcaaaat cgttttttga cgcattccat gtgaaaaact tgcgattgtt
    10921 tcattttgat gactatacat atcaaaaagc gctgtcaatt ctgctaaacg cttttctttt
    10981 ttttgtgtgt ctaattcata ggcagctgaa atcaagtccc aatattctcc agccgtcaat
    11041 tgtaaaaaaa tatctgggga atcaggcaca taaccgattt tttgtttgat actcaatcga
    11101 ttttccgtca atttcattcc atctactaaa atcgttcccg atgatggttc aataatacta
    11161 actaaacttt taatcgtagt tgattttccg gcaccattat gtcctaaaaa tccaaaaatt
    11221 tccccttgtc ttatcgagag agacaagtct tttaatgctt gttttttccc gtaactttta
    11281 cttacattga tgaattcaat catttctgta ctccttttgt ttgttattac tttttattgt
    11341 acccttcgtt attggtggag taaatgtcaa aaagagtgca taacaaaaaa ggatagaaac
    11401 gactaggctg ctacttgggt tgttctcttc tacaggaaaa cactcttcta acaggtaaga
    11461 tatgagtctt tgttttcttt aaaatgattc attttctatc ttttcttgca taaaattctt
    11521 tagtttatca atacttctct ttcccatctc tagttgttct ccatttgcaa atgttactat
    11581 ttcgtttact ctgctaatag atgtaatgtt ttcagtatta atgatataag cttttaaatc
    11641 tttacataaa tactcatatt cgagtgattt tttatatttt ttaattgtgc cgtttacgag
    11701 aatctcttca tctttcaata ctaggcctat tttattttta taacctggca tgctcattag
    11761 ataaaggatt tcatcgtaat ggatatagtg atcgctatta ccataacgga cagttgtcca
    11821 cctttttttc gtagcatgtt gaacaggata gttttctttt tctttcaata agtaactttg
    11881 gatgcttttt ttattttttt cataagtgaa ttcttttatc aaataaccac taggcgtaat
    11941 ctttcgatta ataacagtta ttgctttatt cggatgagac gttagataaa caattttaca
    12001 cttttcattc ttttctctaa gcatttcgcc tagttcgata ccgctataat aggcgttcaa
    12061 atcgatgtct aggaaaaaaa tatctgagga atagatttca ttatttttaa tgaattcaac
    12121 aaattgtata acgttcaatc cctgaacgat ctcaaatttc caaggactta ataaaattct
    12181 ggattcttcc aaaatttcta ctagcatttt ttcaaaaacc aaactatcat ccaaaataaa
    12241 tatacgcgtt accataaaaa gtacctccac tcgatatttt actatcaaaa tcatttcata
    12301 caacttttca acatacatgt taggaataaa ttgtaattct cttcacaatc ttttccaaca
    12361 cctttgaaac cgatatcaaa tactttcagg atgctgcttc taaaataaat atcaaacaaa
    12421 tgaatacatc atgcaaaaaa agccagacaa ccgacgtcct tgcgtgtcag ctgtctgagc
    12481 tatgtttatt tattttgttc ttttaaaaag aacgatacac atactgattt ttcggagcag
    12541 agactttctc agcgtttgta acaactacag atgggatttc acgcttgaat ggttgataat
    12601 agtctgattg gatggttcct tctattttat accattcatc atttttaacc gttactcctt
    12661 ccggcatatg cgtcaacaaa ccaaagactc ctgaatctgc cacacaatgg ataatgccga
    12721 atcggaacaa gaagatatct gtattgctat cgttcggtgt ttggtaaaca aatccttcat
    12781 agctgatttt tttgccaatg aattcgctag ggtaattata aattagctcc ataacttcta
    12841 aatagttttc atctgtgatc ttgatagttt gcttatcccc atacttttct ttcaactcat
    12901 tcatttggtc taagtagtct gatttgttga aataaatact ggtatccggc tttaagtatt
    12961 gtgtctgcat ctccggatcc cctactgatt ctttacttac tgggaaatta aatcctttgg
    13021 cttctacgat cgtagtgtcg agactaactg ttggaaacaa cgtccccacg attactggca
    13081 aagctagcag tacataagcg attccacgtt gataaggttt tgacaaccca tggtcatgat
    13141 cgtgctcgtg tgccttttct tctgcttttc cgtttttgac ccacaaatac agctgaacta
    13201 aagccaaaag agcagataaa atcattgaaa gaatagctaa gtagcgataa tggacattga
    13261 tatattgatc gagtttgcca gaaacttgca agtacatcat caattcgaaa tacccagata
    13321 aaatcaaaaa ccgcagcata ttttccctcc taaataaata atgtgaatcc aataacgcta
    13381 agggcaatca gtccgatcag cgataaaata aagcgtcctg taaaatatcg tttcatcatc
    13441 agtagatttt tgatatcgac cattgggcca aagaccaaaa aggcagctac aggtgctgtg
    13501 ccgaacagac ttaacaaaga agatcctata aacgcatctg cttctgagca aagagacatc
    13561 gtaaaggcca ataacagcat gatgataatg gctaaaatct ttgttgagcc gagcgttagc
    13621 ataattctag ttggtaaata agtctgcatc gcagctgcga ttaatccgcc aatgatcaaa
    13681 taacgacctg tatcaaaaaa ttcgtcgatt gaatgtgtca acacatgccc agtttgatgg
    13741 atcagctgat tttttggttc attcttctca ctttgttctt cttcatggac atgatgatgg
    13801 tgattttctg ctactttaga cgtgattttt tctttcaata cagcgtcttt ttgaaaatag
    13861 gcaagccaga caccaatcac aagggcaaca aaaaagctgc ctatcacccg ccagcttgcc
    13921 atctgccaag aattgccaaa tgcgataaaa gtggaaaaaa tcacgattgg attgatgatc
    13981 ggagcagtca gcataaaagc aaacgctgtg tgaactggta cgcctttttt aacaaattga
    14041 tgaacgatcg gtacgatgcc gcattcacat gaaggaaaga aaaatcctaa gatcgaacca
    14101 gtaaaaatag agaagatacg attttcagga agccatctct tcacacgttc tggggtcaaa
    14161 aatacttgta aggcgcctga gataagacag cctaataata cgaaaggtaa tgcttcaata
    14221 acgatcgata agaaaatcgt actcatctgc aaaaccgaat ttggaataaa agaaaacatg
    14281 agtttcctcc tttttatttc tagttcattc gctttgcaaa tatacgcttt tattcaaaaa
    14341 aaacaagttg ctctgtaaat ttttacacaa ctcttatttt ttaccaaagc acttcaattg
    14401 actttttctg ttattcccat taaattaagg tctcatacat atcacttcta tcataaaagg
    14461 gagaataaaa atgaaacaaa ccactaactt gactgaagtt caagacatac tgcaacagtc
    14521 atccgttgcc gtcatttatc tttcgatgcc taattgcagt gtttgtcacg ctgttcgtcc
    14581 acgactagag gaacttctca cacattatga tattccggca cttcatttgg atgcatttga
    14641 aacaccagaa gtagctagta aatttgaagt gctgactgcg ccagttgtcc tgatttttca
    14701 tcaaggaaaa gaagtctttc ggcaagcacg gttcatcgac tttaaaaaaa tccgttatct
    14761 actagatgaa ctgacagcag aagatgattc cctttcttat gaagaaatct tccgaacaaa
    14821 ataatcgttg tccaaacaga aaaaagcgat tgaacaagtg aaagttttgc cttcatttgt
    14881 tcaatcgctt tttattttta ttctgctgta ttttcggaat gcgcatttgc gtcagaatgc
    14941 aagaaataga tcaaggtttg caattcattc gtcaaatcaa cattttggat caatacatct
    15001 gacggagcat tcaatcgagc agcggtaaag ttcaagattc ctttgattcc tgcttccgtt
    15061 aactgattga ctacttcttg tgctctttca gcgggtatcg ttaaaattgc tacttcgatt
    15121 tgttggatct tgatttgttc tgacatatct tccattggat aaacggggat accgtctacg
    15181 atgcgcccaa cgatttcctc attcacatca aaagcacaac tgatacgtat gctattactt
    15241 tgatggaatt tgtattttag taaagcactt cccaagttcc ctacaccgat cagcgcaaca
    15301 tttgtcaatt gatcgtcgtt taatgtcttt gcaaaaaagt gcataagatc ttctacatca
    15361 taaccatacc cacgttttcc taattcgcca aaataagaaa aatctcgacg gatcgttgca
    15421 ctatctactt gaacggcttc acttaattca gttgaagaca cttttttctt tccagcatta
    15481 tttaaaatcc ttaaatagcg ataatacaaa ggcagtcttt tagctgtcgc ctttggaata
    15541 atatgatctt tcacgaaatg acctccacgt attgtctaat tacatctttg atttctccat
    15601 catagcagta tttataggaa aaaatcaatt ccattgctca gatattgtga aattttattc
    15661 tttattcatt tatttatcat ttctgcaaat ctcgcagtat aatggaattt atggtagact
    15721 actaattgaa aaatagtttt ttatttgagg agaattcaca aagtagaaaa gaggattcca
    15781 tgatattatt acaagccaat cagattgctc gccattttgg tgcagaagtc ttatttgaaa
    15841 acatacattt agaaatcagt acaggcgctc gtgtcgcttt agttggcaga aatggcgctg
    15901 gaaaatcgac tttattaaag atcattgctg gaattgaagc acctgatgaa ggaaagatcg
    15961 ctaaaaataa aacagcgact ctcggatacc tcgctcagga cacaggactt gattccaatg
    16021 aaacagtttg gaacgagatg ctgaaagctt ttgaacaagt tagacaaatg gaagagcgga
    16081 tgcgcgagct agagattttc attagcgaag gtacgccaga cacacctgct tatagttctt
    16141 atttaaaaga atacgaccgg ctgcaacacg atttttcaga tcgaaacggc tatggttatg
    16201 aaaatgaaat tcgttccgta ttgcatgggt ttaaattcga ttcttcattt tatgatcaga
    16261 aaatcgatac tttatcaggc ggacagcgca cacgtttagc tttagctcgc atgcttttgc
    16321 aacaccctga cattttgatt ctagacgaac caacgaacca tttagatatc gagacacttt
    16381 cctggcttga gaattatctg caaagctata ctggtgcatt attgattgtt tcccatgacc
    16441 gttatttttt agataaagta gtcaatgaag tctatgaatt aagcagaaga aaaatgcgcc
    16501 attacaaagg taactattcg aaatatctag atttgaaagc agaacagtta gcaagtgaat
    16561 ggaaggctta tgaaaagcag caaacagaaa tccataaatt agaagacttc gttgctcgga
    16621 accttgttcg cgcctctacc acaaaacgag cgcaaagccg cagaaagaca ttagaaaaaa
    16681 tggaccggct ggatcgtcct caaggggatg aaaaacaagc aaactttcta ttcagtatag
    16741 aaaagccttc tggaaatgta gtcttgcaaa tcgaagatgc cgctatcgga tatgataatg
    16801 tcaccttatc tgaaccaatc aatctagata tccgtcgtca agaagcgatt gctctagtag
    16861 gtccgaatgg tatcgggaaa tcgacgttgc tgaaatcaat cattcaccag atccctttta
    16921 tccgtggaaa agaaacgcta gggacaaatg tcaccatcgg ctattacgat caaggtcaag
    16981 ctgatctgca tacaaacaag acgatcctag cagaattgtg ggatgagcat cctaccactc
    17041 cagaaaaaga gatccgaagt gctttaggtg ctttcttatt ttctggcgaa gatgtagaaa
    17101 aaccaatccc tctgctaagt ggcggtgaaa aaacacgggt tgctttagcc aagttagcga
    17161 tgaacaaaga aaattttttg attttggatg aaccaacaaa ccatttagat attgacaaca
    17221 aagaagtatt agaaaatgca ttgatcgatt atgacggcac actgcttttt gtttcccatg
    17281 accgatattt catcaatcgg atcgcgacaa aagtcgtcga actctctgaa aatggcagca
    17341 aagtctatct aggagactat gattattatt tagagaaaaa gcaggaagaa gaagaaatcg
    17401 ccgaattgtt ggcacaagat gagccagaat ctgctcctgc tcccacgaac aaatctgatt
    17461 attaccaaag caaagaacag caaaagctta tccgtagcct tcaacgaaaa attactcagg
    17521 ttgaagaaga aatggcgcgg atcgatgaat ggatcgatca gttggaacaa gaaatggtcg
    17581 cgccagaaaa cctggaagat cacgtaaaac tcaatgagct gaaccaagat ttagaagctg
    17641 ctagacaaga tcaagaaaca aaattgacgg aatgggaaga actaagcttg gaattagaag
    17701 aatatgaaaa ttaacgaaaa aaccaaaaca gaaaccattc atgctgtttt ggtttttctt
    17761 tttttatttt atcaaacaaa aattccatat ttaccttttt caccaataag aagaccaatt
    17821 actagtcatt ctcactaaag cttctaaata atagtaatca ccccaaatac aaggtgtatt
    17881 taccccatta tttttgggct tattataaac agactgtgtt aaaaagccat cttcttcttc
    17941 cgttgtagca tatctttctt tcaattgtcg cataattttc caaacagcag actggtataa
    18001 gttcttttct gttttgtcta atagacctga ttctattaat tctaataatc cacaaaccat
    18061 gatcaccata ctagatgtat ctttttcttc tctgctagaa tcatcaaaga tcaaatccca
    18121 ataacaaatg gtatcttctg gcaaccgatt cataaaataa ttggctaata ttttgcttaa
    18181 atcaaaatat ttttgttctt tcaaataacg ataacttaat gcaaaaccgt aaatccccca
    18241 tgcttggcct ctagcccaac aggaattatc actataacct tgatgcgtac ttccatatct
    18301 tggttttcca tctttgacat ccatataaaa tgtatgataa gttgaaccat cttctcttac
    18361 taaataatct gctgcatttt gaatatgagc ctgagcagct tttttatatt tttgatttcc
    18421 agtttcttca ctcgcccaat acaataatgg aagattcatg caacaatcca tgatcattct
    18481 accttgttgt gaaggatcac taggatctcc ccaagcttgt ataactcctg ccttctccca
    18541 aaaacgctga agtaatgttt ctgcagcatc aattgaagct tccttcattt ttcgatttcc
    18601 cgttatttta taatctgcta cggttgataa agaatacaaa aaaccaagat cgtgatgatt
    18661 agtgataatt tgacagtcaa tcctgtggat aaaatctata tctgcttttt cagctgtttg
    18721 ttgaaataaa ggatcacccg tcttctcaaa taacaaccaa ttaattcctg tccaaaatcc
    18781 ttctgtccaa ccaatattgt ctcttaatgg ataacgtcct tttataggac actcgcttgg
    18841 gaattgatac tgactgttac tgatacgttt gattaacctc tccgtgtgtc tgcgacaacg
    18901 ctcgatctca ttattccatt ctttttccat taattcttta taacattcat ttatccattt
    18961 ttctggatgt attatctctt caaacatcac tttatcaagc atttccccac cagcctttag
    19021 cataatttta gtcttttttt attgttttta ttttggatag ttagcccggt tgtcccatca
    19081 aaatcaattt tgggacgatt gttccatact tctaaactat aatcccgatc ttgatgagca
    19141 accactcctg ttccgagcca atgggttcct tttccaaatg taccctctaa tccaggaata
    19201 aaagtgtttt cccatgtcat caagtttgta ttgggcaaac ccataatagc tgtggatttt
    19261 cgtttaatag tatcgtcact taaacagata atacctgaaa agccagcttc actcacccta
    19321 tagttttcct tgtcttcttc tatgattatt ccttctttct caggagaaca ttcaatagca
    19381 aaccctgtct catatagaac atattctgta tctaattcta ctttgtgtac gcgaatctgc
    19441 cagccttcaa aaggaataag ccaagttctg atttttgcct gtaaagtagg ttcccatgta
    19501 gaggcaagta cctctttctt acatacaatg tctcgtacat tatttcgaac gatgaactgt
    19561 ccataggttt tgatgctctc attcttaaaa ctggtcgtga tatatgtgtc cgctcgctga
    19621 atccctagca ttgaatccat tgccatttct gctttcaatt gattcgctcg cggtatcgaa
    19681 aaaccaaatt gactagaata agcaaatttt aaatattttt cattggtcaa tgtatggaaa
    19741 tttggcccta attgtcccgc attcaaaaaa aatgtatgtc cattatcatg ttgtatgagc
    19801 aaattggctt ctgaaagaag tttagttcct tcacgaatag gcataggcat ttctttagcc
    19861 gtccaatact catggtcttc aggtagcgcc aacaataaaa aaatcttatt cagccataat
    19921 ggagataacg tactattata gggttcagtc aatgtcaatt gagggtaggt ataaccaata
    19981 ttcaggatac ctttttcatc aaatatttct ttttgcatcc accatcttat attccgattg
    20041 actaacccct tgatttcacc taatttaaaa ggcaaaagat cggcccacaa catagctgac
    20101 caaaagcttg aaactgcaaa acgataaatc atgcttcgac caaaaggcac atttgcacca
    20161 tcttccgcaa aaaaatgaat atagtctcta gaaaaatcaa tagctcggtt cttatatatc
    20221 tggctaatgg gcttttctgg ttctagtacg ctataaatca aaccataata atgaaatgca
    20281 aaaggattat aataatcttg gcgacctctt gaactgtctc gataccagcc attttgaaga
    20341 taacaatttt ctatattatc aaatgcttct ttcagtgctg attctttaac tggataacca
    20401 aatttttgga taattgttcc gacaattatc ttaaaaaatt gccaattacc atctgcacat
    20461 tcatattcaa aaatttgcga gaaccaatga ataatctggc ttttttctcc tgtatctaat
    20521 tttttccata ataattgttc atgatgatat aacgctaata acattgcggg catttctacc
    20581 atacgctggt ctctttcatg tatttctccc caataatcgg gatgggaagg atctgttcca
    20641 gcttttattc ctgatagaat taatttcaac catcgttcat ctaattggtc aattgcgggc
    20701 cccgctcccc ataaaacccg agagaatcct tccattcctg caattttatt tccatatcct
    20761 accccttgat gtatataatc aatttgtgcg ccacttgctc gataaaacgg ctggagtggt
    20821 tgatttatct ttttaaataa gtcaaagtaa tcctgtctgg tccttaatag tggtactgga
    20881 tgtttcattg tattttctcc tctttttact accaaatagc taattccgac tgttctaatc
    20941 gaagtaagcc ttcaataaaa aaataatcag aatagatcag tggcacatta taagtcagtt
    21001 ctctatggta tgcttctgct ccgcctccaa tgatcccatc ttcatccata ttccaatttg
    21061 catagtgctt ttctgtcttt tggagtatat tgatagcagc ttcatgatac atttttgatc
    21121 gtacagagga aacatatcct gctaattcca gaagaccgca tgcagcgcat actccagcac
    21181 tggtatcaat attcgaatct tttacagcaa aatcaatgag aggaatataa ccagtttttt
    21241 cgattttctg aataaaattt actgctatct tttctgctat agctagatag tacggatttt
    21301 ttgtgtaacg ataacttaac gcgaatccat ataaagccca tgcatttcct ctgctccaac
    21361 aagaattctc attgtatcct tgcccgccaa gtaaccgaat atatttccct gattctgcat
    21421 caaattctgc gatatgcccg gttgatccat ctttcctcac tagatactta gctgttgtat
    21481 ctgcatgctg ccgagcaatt ttagagaacc tcggatcacc agtttccctc gaagcccaat
    21541 acagtaatgg taaattcatc atactatcaa taatcatcca accaggttta tggtcattcc
    21601 atgctactaa atagttccct gaaatattat agcgtccagc aagtaccgta gctgcatgta
    21661 accctagttg tcgactatgt ttgttttgag actgttgata atgtgcaata gccgatggta
    21721 agaataaaaa acctacatca tgatgaacgt ctgaaaaatg gtacagggca gattctaata
    21781 attgttcttg ttgtattgct gcctctttga acttttctcg ttttgtcttg tgaaagagct
    21841 gccataaaat accactccaa aaaccattgg tccaccagtc gattcctctg tcctttatta
    21901 cgtctgtata ttttccgttg taagaaatat atggaatttt attccccatt ctgtctaatt
    21961 ccgcatctat cttttcagaa gctacattca aaatttttct tgaccattcc ttcgtctgtt
    22021 cattcatgga tatcccgcca ttcttttaat ttttatgaat gtggagttgc tcctagaacg
    22081 aactagatgg ttaacattcg ttattttttt tacttatttg cagctccaat gtcattctcc
    22141 ttaaatttag actgcgccca cacataaatc aagaccattg ggatcactga agttgctaaa
    22201 attagcataa ttgcgccata attaattggc tcacctgtac tagaaaccgt catatttttg
    22261 ataattaagg gaaccgtata tttatcttta tctgaaagca tgactaatgg aaatagatat
    22321 tgattccaac ttcccataaa gataagtaaa ccactagtaa taatcgtagg tgataaaatc
    22381 ggtacagcga ttttcgtaaa aatcacccac tcattggcac catcgattct ggcagcctcc
    22441 atcagtgctg taggaaattg actgctatat tgtcgcatca taaacactga agttgtactg
    22501 gcaatcgaag ggatgatcac acctgcataa gtattcgata atcctaaacg actcataatg
    22561 taaaataaag gaatcaatgt ggtaaaaaaa ggcaccatcc tagatagcat aactgtattg
    22621 aacaacagtt tattccccat aaaacgatac tttgctaatg cataacccgc cattgtagaa
    22681 ctggctgtag ctgaaactgt actgattaca gcaataacaa tcgaattcca aataatcaag
    22741 ggataattat atctcgccat gatggcttgc cagtttttta atagcatatc cccaaacttg
    22801 aaattgattc cgccacttaa aatttcttga ttactttgcg ttgaagctac aatcatatat
    22861 aaatatggca tcaaaaatat taaggcacca aacacaataa tgacgtacgt aaaaattctg
    22921 ctttgtatct tttgtttagt catttttatc acctccgaat ttaaattgaa tagccgataa
    22981 tataacagat acaaacacca tcgtccaggc aatagcagag gcttttccaa aatcaaccat
    23041 ttcaaaactt gttttatata aattgactcc tacgaccaaa gctgaatttt ctggtccatt
    23101 ggaagtccca aagaatatat ttgggatatt gaaattaccc aagccattga tagtggctaa
    23161 aactgtggcc attaaaatga ttggctttag catggggatc gttaataaaa agaattgccg
    23221 aaatttcgat gctccatcta tttcagcact ttcgtatatc tctttagaaa tcccttgaag
    23281 accagctaaa aataaaatag tgttatatcc tacactaagc caaatcatcg tgatgatcat
    23341 tgatattcgt atccaaaaag gatttgttaa ccactgtaca ccgttaattc ctagcgcatt
    23401 caaaaatcca ttgaataacc cagtcggatt gaaaaaagtc atgaatacgg aagctacagc
    23461 caccgttgat gtaatggttg gaatataata gacagttcta aaaaatcctt ttccttttaa
    23521 ctgtgctgaa ttcaataaaa cagcaaagta aatagctaat agcatcgtta ttggaattga
    23581 tcctgccatg ataatcaaaa cattcgtgta agatttaata aacaatggat ctcggaagat
    23641 agctagataa ttgtcaaatc caacaaattc aaatgagtag ccaattttca taaaactaaa
    23701 agcaaacgag ataatgatgg gaatcaaaat cgtcacaata aagaaaataa caaaaggtgt
    23761 gaccaatcca taagcacttt ttttatgaga cgtatctgtt gcttttttct tgctgttctt
    23821 tattgtcttg cttggtaacg aagattgcat catcgttaag tcctcctaat attttttaat
    23881 caccagttaa ttttacgccg tacttatgag caaaattttc tgcttctttt agatatgaat
    23941 tttcaaaatc acctgtttgc caatagttat atgcagcggt agtcaaataa gacgtagcat
    24001 ctgaataagt ggctggtaaa acagtatttg atgtattttc attgatttta ttcaatagtt
    24061 catagtattt ttgtcctcct tagtagtcaa actttttctc agctgcttct gtttgatagg
    24121 ccaatgagtt agatgtagcc acgcccagct ccagtgcgtt ggctaagcat tcttcatctg
    24181 tcagcataaa tgtaagaaat tgttgtgcca cttgtggatt tttggcatct ttagggacat
    24241 aaaaacttga tcctcctgat actgcacctt ttcctggatt ttcttttgaa aaaggaatca
    24301 gtggagccat gcgccatttg tcagatgcat caggaaaatt caaagacata ttcgtagcat
    24361 accagccgcc agcaacaaac attgcagatt cttgaaatgc tttctcagca tcttgtgcac
    24421 cgtagaaatg tacaatatct gagtcgatca tttctttgat taactttgca gcatgtttcg
    24481 cttctttagt attcagacgg atatttcctt cttcatcaag caaagattga ttctgctggg
    24541 ctagcatgtt gataaacaat tcttggtccc cagattctgg caaggcaatt ccaaaactac
    24601 cagttttttc ttttaatgct tttgccacct ggatatattc ttcccaagaa ctgatttctt
    24661 caaaatctac acctatttca gcaaattttt ttggttgata ataacaaagt gctggggtaa
    24721 aatcactagc ccaaggaata atcgtttcat tactcgtttg ttgacgcaaa atattcaatt
    24781 tttgatcata aaactgatcg ccatatttgt cataaaatcc atattcatca gcactataaa
    24841 aagcatcttg aaatttttcc agcacacctg ctgctgaagt gtctgccatg gagataatat
    24901 ccggcatatc ttcgtgtgtt accaattttg gggtgatcat ttgtactgtt gctgttcccg
    24961 aagctacatt cacaattttt aagtcaatat ttgctccagt tttgcggtta tagatttttg
    25021 cagcattttc aacatagttt gtcagtttgc cgtttgcgac atatacttct aatgtagtgg
    25081 ctttttttat cattggatct gcgatgtttg ctgttacttc ttcgttagtt tttccacatg
    25141 cacttaagct gaataatagg gtaatgttta gcaaagctat caccaatttg tatctttttt
    25201 tcatgtctat cccttctttc ttttcaagtt cttatataaa acgctttcac ttatgtatat
    25261 taacggaatt agtaacacaa atatttatat aaaaaaatat tttacttgtc gttttggctt
    25321 ttcatttttg ttataatagt cattaattat aaatagaaag ctttttatat aaaaagagaa
    25381 aggaaagtgg ttcttttgtt aacaaacaac caatcctcca atcttgtcaa aaaaacacta
    25441 acctattcca tagataccca tcttcattta tctgctgctc cctattttcg actactaata
    25501 aatgaaagcg ctaatttata tgctgaattt gcacataaat ctatagaact agataatcct
    25561 tcccttttat tcattgataa acagcaagaa gtccaaatct tttctactca aaaggaaaca
    25621 caaactttta tcattatcca gttttatttt gatgagcaat tattagcaca tattacggga
    25681 caatcttttt ctatagatca agaaataaca tctatcatta cttttctgaa aaatgaacag
    25741 ttcaaaattg attatctaaa acatagtaag ccagctattt cctatgatca ggaaatcaat
    25801 tcttttttca tactatgcca acagatgagc attcatttgc tttccttatt agttacttat
    25861 tgtctgcaaa aaattcaatt gtcttcctta ccaaggcatt atcaacagga acttgcttta
    25921 ttgacaatac cttcaaatga gaaagtcatt cctaaaaaaa agcatattct ttattcagat
    25981 gaattagttg atccaatcat acgatattta gaagaacatc tttcagaaaa ttttttgctt
    26041 gaacaattag cccaaatttt ttttatcagt ccagccagct taaaaaaaca ttttagaaaa
    26101 gtgaatggaa aaagtgttat tagctatttt aaagaactaa aaatggaaaa agcaaaacgt
    26161 ctcgtgctag aaaatgaatt aacctataca gaaattgcag atatattagg atttcattcg
    26221 atccatcatt tttcttcagc ttttaaaaaa tatacagggt tatccccttc aaagtattat
    26281 ctatccttaa aatagaaaaa aatttttagg ttttatccac aaattatttc tctatatttt
    26341 ctgtctattc aaaataaaca ataattttcc ggcacaatta tttgctgttt tttaccataa
    26401 catagtacga tcaacgtaga aaaggagatg tttttatgaa agttgttgtc attggcggtg
    26461 tagctggcgg tccttcattt gccactcgtt tccgtcgatt gaatgaagcg cacgaaatca
    26521 ttatctatga acgcggagaa aatatttctt atgcaagttg tgctttgcct tattatttag
    26581 gaggtgtgat cactgaccgt gactcgctga tcgaacgtac accagaaata ttgaaaacaa
    26641 aaaacaacat cgatgtcttt actaaacacg aagtaacagc aatcgatcct tctactaagc
    26701 gattaacagt taaagaccta tccacaaatg aagaaataaa aacagattac gataagttga
    26761 tcatctcttc tggtgctaga ccagattatc cggatattcc cggagttttt gaagcagaaa
    26821 acggctttgt cctccgtagt gtgacggatg cggatcgaat caaatcgttc cttgaagaaa
    26881 aaaatccaca acatgtcgtc attcttggtg caggtgttat gggtctggaa ttagctgaga
    26941 atttcaagca tcgcggctta gacgtgactt taatcgatca attgccacaa gtcgctttcc
    27001 cttatgatcc agaaattgct aatttagttt atgacaaatt gctgaaagaa ggattagccg
    27061 ttcatttaga aacaagagtt actgagatcc gtgataaagg tcgagaaata gtattatcag
    27121 atggctccgt cctttctgct gatatgctaa tttttgctgt tggtgtttct ccgaagaatg
    27181 aagtggtgaa agcaaccggc atacaattat cagatacagg tcagatcatt gttgatgacc
    27241 agttacaaac caatctgccg gacatctatg cgattggcga tattatcgaa acgactagtg
    27301 tagtgactgg tcagccgatc caaagtatgc tttccagtgc ggccaatcgt caaggacata
    27361 tgctggcaga tattttaaat ggtacgccta tgcgctatcg cggatatatt ggtgcaggtg
    27421 tcgcaaaaat ctttgatcat acagcaagtt atgcgggaat gacagaacat gaattaaaag
    27481 catcaggcat aacaaattat aaaactgttt ttatcactcc ctttgaccat gcctatttct
    27541 atccagaagc tacaagatta aatctaaagc tgatttttga tgcagatagc ggtcgtattt
    27601 taggtggaca agcatttgga gaaaaaggtg tcgataaacg gatgggagaa ctttctgtag
    27661 caatcaccgg aaacttgaca gtctttgatt tgcctgattt ggagttgcct tactctccac
    27721 cgtattctac tacccgtgac ccgttgaata tagctggtta tgtcgcaatc aatcaaatga
    27781 cgaatatcgt agaaacgatc aaagcaagtg atatacccga aaacgatttg aaagaagcgt
    27841 tctttttaga tatacgtgaa cctaataaag caccttctgg cagtatctcg gcaacaaaaa
    27901 atattccaat gaatgagtta cgagatcgaa tcaatgaaat cccaaaagat aaaaaagttt
    27961 acattacttt cagaagagga ttgaatactt atacttctgc ccgaatcttg gcaggtttgg
    28021 gtatcaaagc gattttgatt gaagaataaa aaaacgaccg cggtgggtag ccgcggtcta
    28081 ggagttattt ttacttgagg agtaaaaatg aaaaagtata tagggttgtt ggttggtatg
    28141 tatatagaat agaagataaa tgtgaagaaa ttgtgcatta tttcttcaca tttattaggt
    28201 tatagtttaa gaaagtttaa acattgcgca atttaattat tttagctgtt tcttttcata
    28261 aaaaaacatg ttgtgactct gagacaaatc atagtccata gtgatgtctc tagagtaact
    28321 acgcctaata ttacgaggca tcatattaat ctatcagcac tagtacctaa actagcatcc
    28381 ctttcgaata ttttttctta tataataaag tttgctttca ctcctctaat ctcaccaaat
    28441 cgactggctt gcgttgtgtg atactaggaa ctgattggtt tagaacgact tttctattgt
    28501 aatcatattt ttcgccatcc tcgttgtctt ctaatccttc aatgaatgat ccctaatgtg
    28561 tgtattaaat aatttaattt ttttgttcct atttattt
//
LOCUS       DACCCS010000036        26539 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.036, whole genome shotgun sequence.
ACCESSION   DACCCS010000036 DACCCS010000000
VERSION     DACCCS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.036"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(99..1229)
                     /locus_tag="IVK82_001637"
     CDS             complement(99..1229)
                     /locus_tag="IVK82_001637"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP6392704.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IVK82_001638"
     CDS             complement(1878..3080)
                     /locus_tag="IVK82_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6392705.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IVK82_001639"
     CDS             complement(3140..3343)
                     /locus_tag="IVK82_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP6392706.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IVK82_001640"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IVK82_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IVK82_001641"
     CDS             4880..5692
                     /locus_tag="IVK82_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6392707.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="IVK82_001642"
     CDS             complement(6716..6868)
                     /locus_tag="IVK82_001642"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392708.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="IVK82_001643"
     CDS             complement(7059..7937)
                     /locus_tag="IVK82_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6392709.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="IVK82_001644"
     CDS             complement(7976..9094)
                     /locus_tag="IVK82_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP6392710.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="IVK82_001645"
     CDS             complement(9091..11190)
                     /locus_tag="IVK82_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP6392711.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="IVK82_001646"
     CDS             complement(11241..12062)
                     /locus_tag="IVK82_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6392712.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="IVK82_001647"
     CDS             complement(12059..12868)
                     /locus_tag="IVK82_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6392713.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="IVK82_001648"
     CDS             complement(12889..13383)
                     /locus_tag="IVK82_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6392714.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="IVK82_001649"
     CDS             complement(13395..13883)
                     /locus_tag="IVK82_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6392715.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="IVK82_001650"
     CDS             complement(13960..16656)
                     /locus_tag="IVK82_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6392716.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="IVK82_001651"
     CDS             complement(17084..18157)
                     /locus_tag="IVK82_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6392717.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="IVK82_001652"
     CDS             complement(18154..18981)
                     /locus_tag="IVK82_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6392718.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="IVK82_001653"
     CDS             complement(18982..19788)
                     /locus_tag="IVK82_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6392719.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="IVK82_001654"
     CDS             complement(19800..20885)
                     /locus_tag="IVK82_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6392720.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="IVK82_001655"
     CDS             complement(20902..21444)
                     /locus_tag="IVK82_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6392721.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="IVK82_001656"
     CDS             21702..22691
                     /locus_tag="IVK82_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP6392722.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="IVK82_001657"
     CDS             22688..23425
                     /locus_tag="IVK82_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP6392723.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="IVK82_001658"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="IVK82_001658"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP6392724.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="IVK82_001659"
     CDS             23989..24621
                     /locus_tag="IVK82_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6392725.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="IVK82_001660"
     CDS             24706..25353
                     /locus_tag="IVK82_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6392726.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="IVK82_001661"
     CDS             25358..26341
                     /locus_tag="IVK82_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP6392727.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8115 a         5605 c         4500 g         8319 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccga
//
LOCUS       DACCCS010000037        26299 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.037, whole genome shotgun sequence.
ACCESSION   DACCCS010000037 DACCCS010000000
VERSION     DACCCS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.037"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IVK82_001662"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IVK82_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP6392728.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IVK82_001663"
     CDS             1995..4661
                     /locus_tag="IVK82_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6392729.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IVK82_001664"
     CDS             5025..5777
                     /locus_tag="IVK82_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6392730.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IVK82_001665"
     CDS             complement(5799..6953)
                     /locus_tag="IVK82_001665"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP6392731.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IVK82_001666"
     CDS             7138..9579
                     /locus_tag="IVK82_001666"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP6392732.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="IVK82_001667"
     CDS             complement(9909..10613)
                     /locus_tag="IVK82_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392733.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10800..11045)
                     /locus_tag="IVK82_001668"
     CDS             complement(10800..11045)
                     /locus_tag="IVK82_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392734.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11062..11913)
                     /locus_tag="IVK82_001669"
     CDS             complement(11062..11913)
                     /locus_tag="IVK82_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP6392735.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12079..13083
                     /locus_tag="IVK82_001670"
     CDS             12079..13083
                     /locus_tag="IVK82_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP6392736.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(13324..13680)
                     /locus_tag="IVK82_001671"
     CDS             complement(13324..13680)
                     /locus_tag="IVK82_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6392737.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            14048..15235
                     /gene="tuf"
                     /locus_tag="IVK82_001672"
     CDS             14048..15235
                     /gene="tuf"
                     /locus_tag="IVK82_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP6392738.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IVK82_001673"
     CDS             complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IVK82_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP6392739.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(18040..18759)
                     /locus_tag="IVK82_001674"
     CDS             complement(18040..18759)
                     /locus_tag="IVK82_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6392740.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            19079..19600
                     /locus_tag="IVK82_001675"
     CDS             19079..19600
                     /locus_tag="IVK82_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP6392741.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            19659..20519
                     /locus_tag="IVK82_001676"
     CDS             19659..20519
                     /locus_tag="IVK82_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP6392742.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            20626..23019
                     /locus_tag="IVK82_001677"
     CDS             20626..23019
                     /locus_tag="IVK82_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6392743.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            23201..25042
                     /locus_tag="IVK82_001678"
     CDS             23201..25042
                     /locus_tag="IVK82_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP6392744.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            25066..26175
                     /gene="rpoD"
                     /locus_tag="IVK82_001679"
     CDS             25066..26175
                     /gene="rpoD"
                     /locus_tag="IVK82_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP6392745.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         8483 a         4679 c         5491 g         7646 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc
    10801 tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg
    10861 tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga
    10921 tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca
    10981 aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga
    11041 tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac
    11101 tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa
    11161 ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg
    11221 ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg
    11281 tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat
    11341 catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa
    11401 aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg
    11461 taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaaatgc
    11521 tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca
    11581 gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga
    11641 agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc
    11701 ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc
    11761 ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc
    11821 gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt
    11881 tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca
    11941 actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt
    12001 tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag
    12061 acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac
    12121 agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt
    12181 cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa
    12241 ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa
    12301 gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct
    12361 agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg
    12421 aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac
    12481 aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca
    12541 ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct
    12601 ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta
    12661 gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca
    12721 gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa
    12781 ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa
    12841 atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa
    12901 gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg
    12961 atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat
    13021 aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta
    13081 taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca
    13141 agagaaggat cggaaaagac gaatataaca aaaaaagagc gtgagacaaa agtaaagaac
    13201 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcttat
    13261 ttcttggagt taaacgcttc tgtcccagcc tctttttatc taataaaatt ttaagaccat
    13321 ttactaatta tcgatttttt ctgccgcatt tttaggcact tcagaagcct ttatttcttc
    13381 ccagcttaat acacctttac gaggattgac tttgagttta agatacgcat tcatctttaa
    13441 tggatgttct ctttcttcgc gaagcgtcac ctgttttttt tcaccatctt tattataagc
    13501 cgcttgcttg taggtataag tcgtgaattt ctctccgtta tctgcttggg tagctgacat
    13561 ttcgccattc gtcgtgattt ttgtataata agcttcgccg ccgtaaaaat aatgataagc
    13621 tgcaaaactt ccgccgagaa atatgcctaa aacgacaagt acgccaatta attttttcat
    13681 gtacttcacc tctttcctta ttattaacga aatagaagat ttcgtctatt gaaagttgtt
    13741 ggcaacagct gaattttaaa cttttcctca ttttgttctt attgcttcaa aagaaagtta
    13801 aggactatct tgaagttttt ctcaaaaaac acattttttt atttataaaa ggtacttttt
    13861 tcgcgtataa gacagacgag tgctcttagt tgactttgac caatattgac gtataataag
    13921 cttagttcga atcttacaaa aagtaagtcg gacgtattct ttttgattta gtcaaaaaaa
    13981 gactataata aaaaggtgag agctgaaatt tttcagtgat ctatattatt taggaggttt
    14041 gtaaattatg gcaaaagaac attatgacag aagtaaacca cacgttaaca ttggtacgat
    14101 tggacacgtt gaccatggga aaactacttt aactgctgcg atcactacag tattaggcaa
    14161 aaaaggccta gcaaatcctc aagactatgc tagtattgat gcagctccag aagaacgtga
    14221 acgtgggatt acgatcaata ccgcacacgt agaatatgaa acagaaaaac gccactacgc
    14281 gcatattgat gcaccaggac acgcggacta tgtgaaaaac atgattacag gggcagcaca
    14341 aatggatggt gcaatcttag ttgtttctgc aactgacggt ccgatgcctc aaacacgtga
    14401 acacatttta ttgtcacgcc aagttggtgt aaaatacctg attgttttct tgaacaaagt
    14461 tgatttagtc gatgatgaag aattgatcga tttggtagaa atggaagttc gcgagttatt
    14521 gagcgaatat ggtttcccag gcgatgacac tcctgtgatc aaaggttccg cattaaaagc
    14581 attgcaaggc gatccagatg ctgaagctgc tattatggaa ttgatggata cagtagatga
    14641 atatatccca acaccagaac gtgatacaga taaaccatta ctattgccag tggaagacgt
    14701 cttctcaatt acaggtcgag gaactgttgc ctcaggtcgt attgatcgtg gtgctgttcg
    14761 tgtcggtgat gaggtagaga tcgtagggat caaacctgaa acacaaaaag cagttgtaac
    14821 aggtgtagaa atgttccgta aaacgttaga ttacggggaa gctggggata acgtaggcgt
    14881 gttgttacgg gggatccaac gtgacgatat cgaacgtgga caagtacttg ctaaaccagg
    14941 ttccattact ccacatacaa aattcaaagc agaagtgtac gtgttgacaa aagaagaagg
    15001 tggacgtcat actccattct tcaacaacta tcgtccacag ttctacttcc gcacaactga
    15061 tgttacagga acaatcacat tgccagaaga tacagaaatg gtcatgcctg gtgacaacgt
    15121 gacgattgat gttgacttga tccatccaat cgctgttgaa aacggaacga cattctcaat
    15181 tcgtgaaggc ggacggacag taggttcagg tatcgttaca gagatcgaag cataagaaac
    15241 gcgactcgaa tattttggta atgaaaaatg tttaaaataa aagagcggtc caaacaggat
    15301 ttctgtttgg accgctcttt gttcttcaat tgtataagaa gagattgtag aagcatgaac
    15361 tttcgtttta cttcagtctc ttttattcat catgagagtg ttcatctatc ggtatgcagt
    15421 ttttaatact ttcttcgtat ccattttttc aaaccactta ttctaccttg tgtctgctca
    15481 actggcgcat tatcaataat cagcaaagca atcataacat atgcaatcca actatctcta
    15541 gaatatagag tataacgtga gacccagttt gtagcgtact tgcttttata ttctcttaat
    15601 ccatggaaag aataaaaatg agaaccaaac tcgtaaacca agtatgcaat cctttcctgt
    15661 aaaaaactct ttctggaagt tccgacattt gaaagagggg ccatccctaa attgaaccat
    15721 tggatatgtt cttctttcat ataggcaaac aaatgcaaga acaagaaatc catactgcca
    15781 gaaggtgctt ttttagaatc atatcgcatc agatcaatcg ttcctacttt attgttcgtg
    15841 taagtaggca tgatcgttgc aaaagcgaca atttcttctt gggtattttt gacaactgca
    15901 ataggcgctc tttgtagata atcttcagag aaaaagccta aagaaaaccc tttttccttt
    15961 cgtgaaccca accaattatc agagatattt ttgaaagtac tcatttgctc agtagaaaat
    16021 ggtggttgta agacatcaaa tgtaaatcct tctcgttcga tccgattcaa gacagcacga
    16081 gtgcccttca tttttttccc tgaagttgta aaactattca ggtctacata agcttcttcc
    16141 cccatcttaa tgaaatcgta gccgaattca tgcaaaatca taacaatctc ttcgcttgtt
    16201 tcgtagaaaa ctggtaaata gcatagtcga tctgtttctt cgataaaagc ttcgattgct
    16261 tctggaaaat cttctttctt accagaagga tcacccatta caatacattt attattgaag
    16321 caagcgaatt ggagaagaac agttggttca ccgtcttttt cataggcaaa catccgctta
    16381 tcttttaaga agatcaattg actatcggag tttcctccat aagttgtaag gatcttcagg
    16441 gccttttctt cattaaaggc ttcgccaatc tgtttctttt ctccttgaag gaaatgaaca
    16501 aatagaacga tcatgaaact aacagcgatg atggccaaca atcccgaaaa ccaaatcttt
    16561 tcagaaggaa ataagaaaaa ggaaataaaa tggtgacgcc gatgagggaa atccggcaga
    16621 ttgtataccc caataacgat gtaaagcaag gacagtgcac caataatgat tccatcgatc
    16681 gtacgccact cccatgaata gatcaattgt tcccgaaata attcgttttt agaagcaaga
    16741 atgatcagaa gcagaataga caagaaaacc accggtgtga aactaaaatc acttaataaa
    16801 acataaagta aagctagaac aatcaagaag attgtaggca aatatgctcg tttcacacgt
    16861 gctgcgatcc ctctgcccat aatgattaaa agaaatccca atagtatact aggaaattgg
    16921 ataataaaat gaaatttcaa tgggttcaaa ctatgaagcc agcgaaactc tgtaaaagct
    16981 tgcgggatag tggcagaaag tactagcatg attccagaaa agtataataa gacaaccacg
    17041 attttatgcg caatttctgt tgctaattgt ttagggatac cagaataacg ctgatcaaaa
    17101 gtggaaccga tatttttgaa gaaaaagacg attccaatca aaaacggaat gatgtagtag
    17161 aacaggcgat agagcaagat ccacacaacg actatttctc ttggcacacc aatagcggac
    17221 aacccgataa tcatcattac atcgaaactc ccaatttctc ctggaatcat agacacgatt
    17281 cctatcactg aagcagcaat gaataatgga attgtttgcc acagatcaat ccggatttcc
    17341 attaaataac caacagaaag gaaactaaac aggacaccgg tccattccaa caatgaaact
    17401 agcaatagct gtccttgagt tttaagtgag gtaccaccaa ggaatccttt ctttttgaaa
    17461 gtagtaaata aaaagacgac aggaaaatat agaccgccgc cgatcaacca tatccaatat
    17521 tgcttcaaaa aaggagcaac tggcgtgaag actacaagta aaaaagaaag cagactgtaa
    17581 atagaaagtc cagccatcaa aaacaagaaa actttcgata atgcttggat cacgtttctg
    17641 ctctctttct tttgtccata tagttcagag cgaagcccca cactaacaaa cccgccgaaa
    17701 ccggcaatat tgttgatcgt attgatcagc cagcttgtct caaacaggta acgaggattc
    17761 tgcttttgtt caagcatccg attcaaaata atatcataac caaccatagg taacacagag
    17821 ataagaccaa tcaatagcat tagtccgctt ttccatacag gtatagtggc aaacgtttct
    17881 cctaattgct ggatagatag agttttaccg atggacatca attcaccgac aataatgaca
    17941 atgacagaaa tcaaaaagat tgttttgaac agtcctaggt ggtttttcag ccattgtatg
    18001 gtatttttta acaaataaat caccttctat tcctaatagt tatgtttcag aaagaaattg
    18061 aagaacttct cgattgaagc gttcaggatc ttttctagct aattcatgtc cttgttcttt
    18121 cacaagcaca aaggaagctt ttggtatagt tttagctata tacaaagaat gttttagctt
    18181 gattacatct ttttttccca cgatgatcaa agtgggggag ttgatttttt ttaaatcatt
    18241 ttctgtaagc ccgatgtcat gcagcaaaag tttgataact aaaagatttt tgcgaagact
    18301 tgggcgaaat aaagagagta accataccca tgcataatga atattactga tgactctggc
    18361 agaaaaacga acgcctttaa caagcgtgtt tccagaattt aaaatcaatc gatgaacttt
    18421 tttagggaag ctgctggcaa ataccagtgc tagatttgct ccatcactaa atccaagaaa
    18481 atctgcctga tcgatttttt ccagcaacat gatcgtattc agatcctcgg ccattaactg
    18541 aaaattcaac atagatgctt cgtttgtcga acgaccatgt cctcggctat ctactagata
    18601 cacggtatag tagcgttcga gtacagggac ctgttctgaa aaaaagcggc cgctgccgtc
    18661 attgccatgc agtaaaaaga gagggaagcc ttgaccgctc ttttcatagt aaatttcgct
    18721 cccatctggc atttttgcat atttcttttc ttttttcaca taatcaccta ctgtgtaaag
    18781 tataacgatt atatcagtca ttgggcaatt gtttttactg aaaaacaacg atcattttcg
    18841 taagatgtca aaaaatacag gaaaattcat tttttcttaa ggcgtacaca gaaatcaagg
    18901 aagaaatagc caagaagata aagaaatagt aagataaaaa aagatgtcaa aataactagg
    18961 tgatgaaatg aagcgaaaac gattatctat tatccgtatt cttttttaca tgatcattct
    19021 gacggttatt ggtgcggtgt tgtttttatg aggaagaact tatcgttcgg taaaacgagt
    19081 ggaaagctat caaatagaaa taacaacagc tgcaaaaaat tatcatatgg aaaactacga
    19141 gaatttgatc aaggcgataa tattgaccga atcaaaagga aaagggacag atctcatgca
    19201 gagctcggaa agtctgcatg gagaagctga gatcattgat aatccagctg acagtattga
    19261 tcaaggtgtc aaatatttgg cggaaatgat agtagaagca aagaaacaag gatgcgattt
    19321 atggacagct gtccaagcat ataattttgg gaaagattat attgcctatg taaaaaatca
    19381 tggcggagaa aacacagtag aattagcaga agcgtattct aaagatgtgc tgagcccact
    19441 tttaggaaat gaggaaggga aacaataccg atattggcgg atccagtcct tgctttacaa
    19501 tggcggctat ctctatcata atggaggtaa catgttttat cctgatattg tcaaaatgaa
    19561 tgaaaaaata atagagacct atgaaaaagt ctttaagtaa aatagcagga aatgttcaat
    19621 catgaataaa attaaatgaa agaaggagtg cgcaaatagt ggaaaaaagg caaaaaatgg
    19681 aaaatcaaga agtggggtac acaaccaaag actggatgtt acggtttgtg aagggaatgt
    19741 ttatcggttc aggatttatc ttgccaggag tcagcggtgg tgcattagca gctgtctttg
    19801 gcatgtatga gcggctgatt tcttttttag cccaccttac aaaaaatttc aaagaaaatg
    19861 ttttgttttt tctgcctgtt ggtctaggcg gagtgacagg catctttcta ctttcttttt
    19921 tagtcagttt tttattaggt gcgtatgaaa caacgatatt atggtttttt gtcggatgta
    19981 tcgctggaac tgttcctgcg ttatggaaag aagcagggaa aaaaggaaga gatacttctg
    20041 atgtgatcat catggttgtt acttttgttc tggcttattt gtttttatta tacggcgctc
    20101 aattatttga cgggcaagtc gatcagaatt ttggaacatg gctgattgcc ggcggattga
    20161 ttggtttggg tatgatcgtc ccaggattga gcccttctaa ttttttggtg tatatgggat
    20221 tgtataaagc gatggctgac ggatttaaag aagtacgact tgaagtgatt gtgccgatcg
    20281 gaattggtgg gattctttgt gttcttggat tgtcaaaaat catggattac attttctcaa
    20341 aggcatatag caagctattc catttcattt taggtgtagt ttttgcttca acgattatga
    20401 tcattcctac cgattacgcg ggattgggag ttgctggggt actagcttgt cctgttcttt
    20461 ttatagccgg tacagcacta ggttggtgga tgagccgttt ggaggaacaa tataagtaaa
    20521 gatagattga taataaaaac agcacacggg tgattcaaac aaaaaggttg gcatcttttt
    20581 tgtaaaatca ttcgtgtgtt tttctataaa ttttataaaa acaagttgtt tttaatcttg
    20641 gaacacgcta gacttaaaca cgtgattatt tttgaaagtt ggcggaaaat ggagaaaaag
    20701 aaaatgaatg gacttacaca gcaagaggta gttcagaaaa tggcttctgg ccagcaaaat
    20761 gactaccagg aagatgcatc aaaaagtaca aaacaaatat tcagcgataa tatattgacg
    20821 ttattcaatt ttctgaactt tggtattggg atttgtctgt tatttgtagg tgcgtattcc
    20881 aacatggcgt atttagcaat tattgtcgtc aatatcgtta ttggaattta tcaagaaata
    20941 catgcaagaa atcttgtacg gaaattatcg attgtttccc aatcagaagt actagtgatc
    21001 cgggacggta aagaacaatc gatttctact aaagagctgg tattaggaga tttggtaaaa
    21061 atcggagcag gagaacagat accagccgat ttacaggtta tatctggacg ggcagaagca
    21121 aatgaagctc ttttgacagg cgaatctgat ttgattgtca aagaaactgg agctgaattg
    21181 ctttctggaa gctttttgac aagcggttct gtcatggctg aagttatccg agtaggtgca
    21241 gataattatg ctgtccgctt gactagcgag gcaaagactc acaaagctat tcgttctgaa
    21301 cttgtagatt cgattcgcaa agtatccaaa ttcactagtt tcgtgattat tcctttgggg
    21361 atcatactat ttttggaagc actgttcatc cgcgatgctt ctatcaaaat ttctgttatt
    21421 gcatctgcag cagctttgtt aggcatgctt ccaaagggtt tggtattgct aataagtatt
    21481 gcattaacga caggggtaac caaactggca aaaaaacgaa tccttgttca agacatgtat
    21541 tcaattgaaa cgctggccca tgtcgataca ttatgcttgg ataaaacagg gaccattact
    21601 gaagggaaaa tgagtatcca gcgggtagaa gcactacatc cgctgtacga gaaagccatc
    21661 ccagaaatta tggggacgta tctggcagaa agcagtgata acaacatcac tatgcgggct
    21721 atgagagatt attttgagct ttctaatcgg tatgatgtga caaacaaagt cgcattttct
    21781 tctgatcgca aatggggcgc gatggaattt ccagaactgg gagtcgtgta cgtaggagcg
    21841 cctgaacgtt tggtggaaga agatcggctt ccggacagtc taaaaacagc ccaagcgaat
    21901 ggctatcgcg tacttatgtt ggggatcgct ccaaatcagc aattgaacga agaaaaacca
    21961 gcatttgttg agccggttgc tctgtttgaa atcgatgatc caatccgaaa aaacgctcaa
    22021 gaaacactag cttatcttca tgaggaaggt gtggatctga aagtgatttc tggagacaat
    22081 ccttttactg tctccaatat cgctagacgt gcaggattac cgaattatga tgcgtatgtc
    22141 gatctttctc aaattactga agaaatggaa gtgcgagaag ctgctcatcg ctatacggtt
    22201 tttggacgag tgagtccgca gcaaaagaaa ttgctggtaa atgaattgaa agaaagcggc
    22261 agaacggttg caatgacagg cgacggtgtg aatgatgttt tggcgttaag agaagcagat
    22321 tgcagcattg ctatggcaga aggtgatgga gcaacacgtc agattgcgaa tctggtattg
    22381 ttggactctg attttactac attgccagaa gtcctgtttg aaggacgccg agtggtcaac
    22441 aatgtgacga aagtctcagg catctttttt atcaaaacga tttattcatt tattttatcg
    22501 attatatgtg ccgttacagc aattggattt ccgtttatcc ctatccaaat cacgctgatt
    22561 gatttagcga ttgaagggta tccttccttc tttttatctt ttgaacaaga taaaagaaaa
    22621 atcacttacc gttatttgcc tacagcactt attaatgctt taccaaatgc cttgcttgta
    22681 gtagtgaata tcattgcggt gtatctactt ggaaaaacat tcgcatgggg aaatgctgaa
    22741 actactactt tgatgtatta cttgctgatt gggatcagtt gcatggcagt tgtaaaagct
    22801 tgttatccct tcaacccatt gcgcatcttc ctagcagtaa cgacgatcgt agggatatat
    22861 gtagctgcaa tgctgttcca tggaattttg gaagtaaact tagggttggg agagagcgta
    22921 ggatacttct ttgtattcat gctcgtaaat attgttcttc gagtattgta ctggaaggct
    22981 gatattcaaa atcagttact gaaaagagta gcaaattgat gcttaatgag atagaaatgt
    23041 gttatacttg aaaaagcaat gaaatatgat acaatcaaac atgtataatt gtgtccttta
    23101 aaatcgcact gatttcagtt agtgcgattt tttaccggtt attctcctga aatatcttct
    23161 gttaaaccca aaagatatca atagagcagg gggtgaaaaa atggctcaac ggattcctca
    23221 agaggttatc gaaacagtac gcagtcaaac gaatatcgtt gaggtgattg gacaatatgt
    23281 ccagttgaaa aagtctggta aaaattactt ggggctgtgt ccttttcatg aagaacgtac
    23341 accgtctttt tctgttgcag aagacaagca gatttttcat tgctttggtt gtggtaaagg
    23401 cggaaacgtg tttacttttc ttcaagaact agaaggtcta agttttccag aagcagtggt
    23461 aaaagtggca gacttcgaac agataccgtt agaagatcaa tggcgtcagc gtactatagc
    23521 tgtccaaaat cctgaatctg ctacggggaa actgattgct gctcatgaaa aagcagcgga
    23581 aatctatcat cacatgctat tgcatacaaa agctggacag ccagctttag aatatttgca
    23641 aagtcgtggg ttgtctttag aattaattga agaattcaac ataggttttg cacctaatga
    23701 gcgttctttt cttcagcaag tattcaagaa tgaatcaatt tcaaatgaat tgttagaacg
    23761 ctctggattg tttgtcacgc atgatgatgg ccgtatgtct gatcgttttt atcaaagagt
    23821 gatgtttcca cttcgtaatt ctcaaggcca gacgatcggt ttttcaggac gctggcttgc
    23881 acctgaaaaa gggcaggaaa aagatcagcc caaatacttg aatagtccag aaacagaact
    23941 gttcaataaa agacaagttt tatttaatct tgataaagca agaagcgata tccggaaaaa
    24001 cggagaaatt ttgttgttcg aagggtttat ggatgtgatt gctgcatggc aggctggtat
    24061 caaaactgga ttagcttcta tggggacaag tctaacagct caacagatcc aacaaatgga
    24121 aaagctgacg aaagaattgg tcttttgcta tgacggagac aacgcaggtt ttgaagcaac
    24181 caatcgaggg attgaattgc ttcggcaaaa cagtcgatta gcactttctg tcgtagtcgt
    24241 tccagaaaag cttgatcctg atgaatatct ccgtaagtat ggagaagcat ctttccaaga
    24301 attagtggct catgggcgcg aaacagtctt tacttttaaa tcacgttatt taaaacaagg
    24361 gaaaaattta gataatgaaa aagacaagat cgaatacctc gaagaattac tagttgaact
    24421 tagccgagtt gtttcaccaa tcgaacaaga tatgtatcta tctcaactgt caacagagtt
    24481 tcagatcggc agagagacga tacaaaaaca actgcgcgat cttcgaagga caaatcagca
    24541 aaatcgtcaa gaacctcagg aaagtcaaca cactcaggtt acgagacaag aaactgcacg
    24601 gaaaaagaga cctttgacgc aagtagaaaa ggcagagcag attctattat atcgtgcatt
    24661 taaagaagtt tctgtccgta atattttaaa agaacgagaa tttcagttta ttcatgatgc
    24721 atatgcagaa gtttattttt tattcgatgc atatgtctca caacattatg aattcaacct
    24781 tgcagagttt ttagactttt tgcaagatga aaatttaagg cggttggtgg tggaaatcga
    24841 atatttaccg gtggcagaag aaagttcgcc acgagaaatc caagacttgc tagatgtgat
    24901 cagcaaatct ggcttagctg atgaaataac attaaaaaaa caaatgcagc aagaagccag
    24961 acgaactgga aacaaacagc ttgagcttga attgacgatt gaaatcatca atttaacgaa
    25021 acagttaaaa caagccaaat agctactcaa ggggggcctt cttttatggc aaatggaaca
    25081 gataaaaaaa cgtatgaagc agcagtagca gctttcatca aagaaaacaa acccaaacga
    25141 caagttgtat atgatgattt atccaatcag cttgctacac cattcacttt gaatgcagat
    25201 gaaatggaca aattgatcca aaagattgaa gacgcaggta tcagtgtggt ggatgaaaat
    25261 ggtgatccat caattcacag cttgaagagt gctgagaaaa aagctgaaca agcaaaaaca
    25321 gaagatcttt ctgcgccaac cggtgtaaaa atcaatgacc cggttcgtat gtatctgaaa
    25381 gaaattggcc gtgtctccct tttaactgcg gaagaagaag tagcgttagc attgaaaatc
    25441 gaagaaggcg accaagaagc gaaacagcgt ctggcagaag caaaccttcg tttagtagta
    25501 agtatcgcta aacgttatgt aggacgaggc atgcagttcc ttgacttgat ccaagaagga
    25561 aacatgggac tgatgaaggc agttgaaaaa tttgactacc gcaaaggatt taagttttca
    25621 acctatgcta catggtggat tcgtcaagcc atcactcgag caatcgctga ccaagcacgt
    25681 acgatccgta ttccagttca tatggttgaa acaattaata aattgatacg tatccaacgt
    25741 caattgctgc aagaccttgg aagagaacct acgccagaag aaatcggtgc ggaaatggat
    25801 cttccaacag aaaaagtccg agaaatcttg aaaatcgcgc aagaacctgt ttcccttgaa
    25861 acgccaatcg gtgaagaaga tgactctcac ttaggtgatt tcatagaaga ccaggaagca
    25921 acaagtccag cagaacacgc agcttatgag ttactaaaag aacaattgga agatgtacta
    25981 gatacattga ctgatcggga agagaatgtc cttcgcttac gttttggttt agatgatggc
    26041 cggacgagaa cattggaaga agtagggaaa gtatttggtg ttactcggga acgtattcgt
    26101 cagatcgagg ctaaagcatt aagaaaattg cgccatcctt ctcgttcaaa acaattaaaa
    26161 gatttcttag aataagcgtg gactattgac cagaagagaa atcagtaaaa ctgtttggtt
    26221 tattttaaaa gaaaaaaagc ttgagactac gcagtctcaa gctttttttt ctataggaag
    26281 gaaattcatt cattatttt
//
LOCUS       DACCCS010000038        26026 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.038, whole genome shotgun sequence.
ACCESSION   DACCCS010000038 DACCCS010000000
VERSION     DACCCS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.038"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="IVK82_001680"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="IVK82_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP6392746.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="IVK82_001681"
     CDS             808..1242
                     /locus_tag="IVK82_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP6392747.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="IVK82_001682"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="IVK82_001682"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP6392748.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="IVK82_001683"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="IVK82_001683"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP6392749.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6192..8423
                     /gene="pflB"
                     /locus_tag="IVK82_001684"
     CDS             6192..8423
                     /gene="pflB"
                     /locus_tag="IVK82_001684"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP6392750.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8667..9428
                     /gene="pflA"
                     /locus_tag="IVK82_001685"
     CDS             8667..9428
                     /gene="pflA"
                     /locus_tag="IVK82_001685"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP6392751.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9513..10439
                     /locus_tag="IVK82_001686"
     CDS             9513..10439
                     /locus_tag="IVK82_001686"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP6392752.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10621..11514
                     /locus_tag="IVK82_001687"
     CDS             10621..11514
                     /locus_tag="IVK82_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP6392753.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11648..11773
                     /locus_tag="IVK82_001688"
     CDS             11648..11773
                     /locus_tag="IVK82_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6392754.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11907..12539)
                     /locus_tag="IVK82_001689"
     CDS             complement(11907..12539)
                     /locus_tag="IVK82_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392755.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12687..13229)
                     /locus_tag="IVK82_001690"
     CDS             complement(12687..13229)
                     /locus_tag="IVK82_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP6392756.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13363..13752
                     /locus_tag="IVK82_001691"
     CDS             13363..13752
                     /locus_tag="IVK82_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392757.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13756..14520
                     /locus_tag="IVK82_001692"
     CDS             13756..14520
                     /locus_tag="IVK82_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6392758.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14649..15596
                     /locus_tag="IVK82_001693"
     CDS             14649..15596
                     /locus_tag="IVK82_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6392759.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15726..16466
                     /locus_tag="IVK82_001694"
     CDS             15726..16466
                     /locus_tag="IVK82_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392760.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16757..17425)
                     /locus_tag="IVK82_001695"
     CDS             complement(16757..17425)
                     /locus_tag="IVK82_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6392761.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            17559..17846
                     /locus_tag="IVK82_001696"
     CDS             17559..17846
                     /locus_tag="IVK82_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392762.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            18262..19800
                     /locus_tag="IVK82_001697"
     CDS             18262..19800
                     /locus_tag="IVK82_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP6392763.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19797..20762
                     /locus_tag="IVK82_001698"
     CDS             19797..20762
                     /locus_tag="IVK82_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6392764.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20749..22071
                     /locus_tag="IVK82_001699"
     CDS             20749..22071
                     /locus_tag="IVK82_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6392765.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      22134..22238
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22312..23055
                     /locus_tag="IVK82_001700"
     CDS             22312..23055
                     /locus_tag="IVK82_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6392766.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            23070..24062
                     /locus_tag="IVK82_001701"
     CDS             23070..24062
                     /locus_tag="IVK82_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6392767.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            24074..24811
                     /locus_tag="IVK82_001702"
     CDS             24074..24811
                     /locus_tag="IVK82_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6392768.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24801..25523
                     /locus_tag="IVK82_001703"
     CDS             24801..25523
                     /locus_tag="IVK82_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP6392769.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(25607..>26026)
                     /locus_tag="IVK82_001704"
     CDS             complement(25607..>26026)
                     /locus_tag="IVK82_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP6392770.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         8953 a         4426 c         5428 g         7219 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggtatgt caacgttgat
     6061 ttaaatatat gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa acccttgcaa
     6121 agatagggtg ctgtgttata ctaatcacgt aaaaactgtt acataaaaca gaaatttagg
     6181 aggtccatac aatggagcaa tggaatggat ttaaaggtac caaatggaaa gaaggcgtag
     6241 acactagaga ctttattcaa aataactaca ctgaatatcg cggtgatgac agctttttgg
     6301 aaggcccaac agaagcaaca gaaaaattat gggatgaatt gcaaaagtta aatgatatcc
     6361 aattcgagcg taacggtgtc tatgatatgg acacagaaat cgtatcaact attacttcac
     6421 acgatgcagg ctatttggat aaagatttgg aaaaaatcgt cggtctacag acagacaaac
     6481 ctttgaaaca agcattcatg ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa
     6541 gcaatggcta tacaccaaac gatgaattga cacatatttt cactgactgg cgcaaaacac
     6601 ataatcaagg tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa
     6661 tcattacagg gcttccagac gcttacggtc gcggacgtat catcggtgac tatcgtcgtg
     6721 tggcattgta tggaatcgac tatttgatgg aacaaaagaa aaaagatcat gatatgactg
     6781 gttatcatac aatgagtgaa gaagtgattc gtttacgtga agaaatcact gaacaatatc
     6841 gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt tctaaaccag
     6901 ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg atatttagca gcaatcaaat
     6961 cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc attccttgat atctatatcc
     7021 aacgtgattt ggataatgga gtaatcacag aaaaagaagc acaagaattg atcgaccact
     7081 tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc tgaatataac caattattct
     7141 caggattccc tatctgggct actttatcca ttgcaggtat gggattagat ggtcgttcat
     7201 tagttacaaa gaatgatttc cgtctgcttc atacattaac aaatatggga ccttcaccag
     7261 aaccaaactt gactgttttg tattctgaac accttccaga aggattcaga acatatgcta
     7321 gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa tgataatcag cttcgtgaac
     7381 aatggggttc tgatgattgt gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag
     7441 acatgcaatt ctttggcgct cgtgctaact tggctaaaac actattatat gccatcaatg
     7501 gcggagtcga tgaagtgact ggcgcccaag ttggaccaga atatcgtcca atcacatcag
     7561 aaatactaga ctttgaagaa gtgaaacata acttcatgaa tatgatggat tggttagcag
     7621 aactatatgt caatacgttg aacatcattc attatatgca tgataaatat tcttatgaag
     7681 cagctcagct ggctttgatg gattctgact tgcaacgcac atttgctaca ggtattgctg
     7741 ggatctctca tgcggcagac agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc
     7801 gtaatgaaaa aggaatagca gttgattttg aagtggatgg cgacttccct aaatacggaa
     7861 atgatgatga ccgtgcagat gaattagttt catggatctt ggaatacttc atgggacaaa
     7921 tcaaacgtca taaaacttac cgcaactcaa aaccaacaac ttcattattg acgattactt
     7981 caaatgttgt ttacggtaaa aatacaggaa atactcctga tggccgtgaa gctggaaaac
     8041 cacttgcgcc aggagcaaat ccaagttaca atgcggaaca aagtgggtta ttagctagcc
     8101 tgaactcaac agctaaaatg aattacggct gtgcattgga tggaatttca aatacccaaa
     8161 caatcaatcc ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg
     8221 tcttagatgg atacttctca aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg
     8281 atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg atccgggtat
     8341 ctggttatgc agttaaattc cgtgacttga caagagaaca acaattagat gtcttgatga
     8401 gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa
     8461 aggacgttga atagccatcg catcaaaatc gaataatcag tatgcttatt tgaaagaaac
     8521 gatttacagg ggcaggactt gtatgttccg tccctgtaaa ttttacacat aacgaagcgg
     8581 caattgcgaa aaattttgtt cagaaagctt cgcttcgaca gctaacttgt acttaaaatg
     8641 aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac aatcggctat gttcattcta
     8701 tcgaaacttt cggttcagta gacggacctg gactgcgctt tgtcgtgttt atgcaaggat
     8761 gccgcatgcg ttgccaattt tgtcacaatc cagatacttg gaatattggc ggagggaaag
     8821 aatacacagc agatgaactt ttagataaag cagaacgctt cagaccttac tggggagata
     8881 aaggtggtat tactgttagt ggtggagaac ctctattgca gatcgatttt ttgatcgaac
     8941 tatttaaaaa agcaaaagag cggaagatgc atactacatt ggatacatgc ggaaaacctt
     9001 ttacgtatga agaacctttc ttttcacgtt ttcaagaatt gatgaagtat acagatttac
     9061 tgttatttga catcaaacac atcgataatg aagagcataa aaaactaacg cattgggaca
     9121 atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg gtttggattc
     9181 gccatgtact agttcctgag cgaagtgatt atgatgaata tttgatccga ctagacaact
     9241 ttattcaaac cttgtcaaat gtagacagag tagagattct tccttatcat acgatgggaa
     9301 aatacaagtg ggaaacactt ggtttgaaat atccattaga agggatcgaa ccacctacaa
     9361 aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga agagtataca aaatatttaa
     9421 cgcgataggt cgcatcggaa agaagataag tattgcttct ttccgttttt ttgctattat
     9481 taaataggag aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt tttggtcacc
     9541 aaaatccaga tacagatgca atcggggcag ccattgcgtt ttcacacttg caaaatgaat
     9601 taggtaaaga aacagaagca gttgctctag gtacacctaa tgaagaaaca caatacgcat
     9661 tagaccactt tcagctatct gcaccacgag tagtagagtc agctaaagca gaaaatgcag
     9721 atgtcatgct tgtggatcat aatgaattcc aacaaagtat ttcagatatc agcgaagtaa
     9781 atattttagc tgtagtagac caccatcgta ttgcaaactt tgaaacagct aatccattgt
     9841 actatcgtgc agaaccagtt ggatgtacaa gtacgattat tttaaaacta tataaagaaa
     9901 accaagtaac aattcctaaa gaaatcgcag gaatcatgct ctcagctatc atttcagata
     9961 cattgttgtt caaatcacca acatgtacgc ctgaagatga agcagctgca aaagaacttg
    10021 cagcaatcgc tgaagtcgat ctaaacactt atggacttga aatgttgaaa gcaggaacaa
    10081 atctaagtga taaatcagct gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg
    10141 gcagttccaa cgttcgtatc gctcaagtca atacagtgga ccttaatgaa gtgatggatc
    10201 gacaagcaga gctagaagca gcaatggaag atgaaatgaa agcaaatggc tacgatctgt
    10261 tcgtattagt tgtcacaaac atcttagaca gtgattcaga attacttgtt gctggtaaag
    10321 gaaaagaaaa agtagaagaa gctttccaaa caactttgac caacaaccgt gctttgctta
    10381 aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta tttagctaat
    10441 caaatggtag tatgaagcat tcaaaatgaa tggataagcg attgatataa aaagaaacat
    10501 agaacatact aaaggccgaa gaaatggagg agaataacag tcaattatga ctcttgttct
    10561 tcctcctttt tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa gagaacatat
    10621 atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga tgatccgctt
    10681 ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat tctaagagaa
    10741 ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt gatcgatgca
    10801 ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt tgatcctaaa
    10861 gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt gaagagactc
    10921 tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg tttcgaagat
    10981 gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga attgctgagg
    11041 acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga acacttcccg
    11101 cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac gaaggcattc
    11161 aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga tcaaatagaa
    11221 gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag tattttccat
    11281 cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt agaattacct
    11341 attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc attgacaatg
    11401 gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa atttataccg
    11461 catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac ctaagagagc
    11521 agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt tatggttaaa
    11581 agaattattt gagtattttg cctatctacc acctattaga ttcactacat ggtctggaaa
    11641 caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag tgataagaaa
    11701 agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt tatgggaaga
    11761 actagctgat tagattagga agtccatttg ataatccaga atcactaaga aacgaatggc
    11821 gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg tttaaaacta
    11881 aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt ttgtttgttt
    11941 tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa cagatttcct
    12001 gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa aaaggaaaac
    12061 aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca tttcaattgg
    12121 ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac taaaccattc
    12181 atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc taaaaaagaa
    12241 aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg atgtcgatga
    12301 aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga caaaatcaaa
    12361 aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat
    12421 gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa taagatcgtc
    12481 aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg aatttccata
    12541 atatctccaa attaaattga taagtatata aagtataata acaaattgat gagtatatgt
    12601 cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat ttaatttaga
    12661 tattagacag tttttaagtt tgtgagttat tttccttgtc gttttctcac ttttttagtc
    12721 cagaaaccac ctgaaatata ttttggctca taagaaatga taaaagcacg ctcttcgatt
    12781 tctttgatca ggcgatacag ttcacgttcg ctttttctag gagaaagtat ttcgagaact
    12841 agccgctcac cttccagccc cattgcagaa gtctgtgtca caccataacc gtgttctctc
    12901 agtatacccg gcaaactttt atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg
    12961 atgtatccta atgccaattt gtcttcaatc aaaattccga cactgatacc tactgcgtaa
    13021 cctaacgcat agacaaataa attcagtgga ttatctaaac gatctaaaac aagggataag
    13081 ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg gagcaatcag tcgatagcct
    13141 ttcattgtca acatgaaacg taacgtgttc agagtgatat aagcaaaatt aatgaggaag
    13201 atcatcccca acatttttgg atcaatcatt tgtaaaactc ctttcattta tataacaaca
    13261 atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt atacatacaa aaaatgataa
    13321 aatgattata atagaattat aaagataggt taggtgatta aaatgaaaac ggtcaatcgt
    13381 gtgttttgtg ccagccacgt gctaagtctc cttctcagta tatacttagg tgtctattcg
    13441 attcaacagc caattgaggt agagaacatt gtttttcaga tgtcgctgac agatggttta
    13501 attttaagcg tttttttctt tttgatactg tttattggta atattttcgg tagcgttgcc
    13561 agttttttga atttttctat ttatccactc ctttcgctag cattaggagt ggtgggactt
    13621 tgttctctgt gcttatttcc cgagccattc agtcctgcat ttatattatt tggtacactg
    13681 aacctgttcc agacaactgt tggagcatgg ttgctatggc gatcaggaaa tttaatgaaa
    13741 ataggtgagt aacaattgaa tccaattata ttgaaacgtt ctgtccatca tattccagtg
    13801 ttagaagttg ttctgaaaga attaagagag gaacgtattc ccgttgtcat ctattatcac
    13861 ggatggcaga caagtaagga gttagttttg acgcaaggaa gaaaaattgc gcagcagggc
    13921 attcgtgtga tcctgccaga tgcgatgaat catggggagc gcaaacagcc ggtttcttct
    13981 attccatcct atacattttg ggatagtatc tacggaaatc tgtttgaatt tgacacactt
    14041 atcaagcatt tagaaaaaag aggtcttcta catgatcagc ttgcagttgg cggggtttcg
    14101 atggggggaa tgacaacatg cgccttgttg gctaaacatc ctcatatagt agcgggcgtg
    14161 tgtttgatgg gttcccctgc acctttggaa tatggaaaag aaatcgtaag gcgcgcatcg
    14221 gaatataatt atgtcgtacc aaaagactat tttgatttga tttcatgggt taagagctat
    14281 gatttgtcat tgcagccgga aaagttagct ggacgtcctc tgttcttttg gcatggacaa
    14341 gctgacgaaa aaatccctta tatacaaacg gaacgatttg tagaaaaaaa tcggcataag
    14401 tcttacggaa aagaaattgt ttttcgttcc tcaaaaacac agcgacatat ggtacaaata
    14461 ccactaatgg aagaagcttc cactttttta gcaaaaaatt tgataaaaaa gtataaataa
    14521 ttgaaacgga aaatcactca tgacgataga ggattttccg ttttttattt tgggctataa
    14581 taaactctgt taggaaaatt tggtataatt gatagtaaga atgacaacat gttagaaagg
    14641 aagagagcat gcccacttta gcagatgttg caaaacgtgc caatgtttca aaaatgacgg
    14701 tctctcgagt catcaatcat ccggaacagg tgactgacga attgaaagag ttagttttct
    14761 cagcaatggc tgaactggat taccgtccaa atattgctgc aaaagcactt gtcacgaatc
    14821 ggtcgcagat catcaaatta tttatactgg aagaaatcga cacaactgag ccctattaca
    14881 tgaatttgct gatgggaatc gctaaacgga tcggtaaagc gcattattct ctccaattag
    14941 ttacaaatga tgccttcgac gtcggttctt gtgatggata catcatcaca ggtgtcagaa
    15001 aaaaagactt cgattggatc gaccgtttgg aaaaaccagt tgtcttgttt ggggaaaatt
    15061 ctgaaggata tgattatgta gacagcgata atgaatatgg tacagcaaaa gcaacgcaat
    15121 atgccttaga aagagattat caaacgatta tttatatcgg tatcgaagtg gacgagcctt
    15181 ttgaaatttc aagggaaact ggttatttat ctgtgatgaa ggaagttgat cttcctagta
    15241 aaatcttgcg gtttccaaat cattcgacag aagtagaaag atatatgaca gaaaactggg
    15301 cagattttac agataaaact tgttttgtat gtagttcaga tcggctagca ctaggagcag
    15361 taagagggat tataaatgct ggaggacact tgcccgaaga atttggtgtg attggatttg
    15421 atggggtatt tttagaccaa atcagttcac ctaaattgac aacgatcaag caagatatta
    15481 tacgtatggg tgaagtatgt ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga
    15541 gtcaaggtta tcgtcgtttt tttcctgaac tagttatcag agagacaacg aattaagtct
    15601 tattactata tatatattta tgaaattcat cgctattttc ttaaaataat cagcgcattg
    15661 ctactgaaaa caatgaatct gtgctattat tgagaaaagt tccatttttg agaaggatgg
    15721 tttgaatgaa agaaatcaga cgaaaacatt tccgttttcc tgcgtacgat gatgagttag
    15781 gcgtaaaatt aaccaacgac aagaagcgag tattatttga cggacaagaa gatcttttga
    15841 ctgaacagaa tcagtccccg tttgaacaga tgaaagaatt tcatttcacg aacacgaaca
    15901 ccgagaaaat ccgtcgcaag cgtcgtgcaa caaatggatt gaagtcagct ccgcgtaagg
    15961 aaaatttgga aaagcataaa ggaaaattac cggattatac cggccattac caatcagaaa
    16021 tcacatcgac aggacgaaaa aaactttttg gtgattcctc tcgttctgtc agacaaagtg
    16081 aaacaaggac agagtccaag catactacac gaaacgcatc tgttgacacg aaggtgcggg
    16141 agcgttctta ttttgttcct aaatatgtac ccgcatcgat tatcccagac cctagagaaa
    16201 gcaaaatttc tgaggatcat ttaatcgaat cgatgaaaaa aacggatgac tcttatctgt
    16261 tgttcgatac agaaccgacg ccgtatcaag aaaaaagaaa tggtgagccg tctgtacgaa
    16321 cattcaatcg aacaaaaacg ttagaggaaa caaagcagtc agaagaaaaa aaatcgaaaa
    16381 aaagccatgg tatcttagaa cgctcattac aaggattgat cgatgatcaa ggaaactctt
    16441 tgggagaaaa tagctatttt aaataagtga tatacaaatt tatattcggc tacagttgga
    16501 aaggaaacgc tgcggcgttt ccttatttta tactctatcc gaaagtttat atcgtatcct
    16561 caaatttttt tgagtttaag cgaatttttt ctcccaataa gtaagaaaat aatggcaaaa
    16621 taaaaaaaca aactattttc gcagatcgtt tgcaaaaata gtttattttt ttataaagaa
    16681 gattttactg aataatatca ttttagttta cataatgata tttctgtaaa ttaaataaag
    16741 tagtgaaaaa taagaatcag tgctctaact gttcaagagg atcaagtggt tcaaatgttt
    16801 ctgtagtctg ttttgcttcc aattggggca aaacagatcc aatttccaga tacttcactt
    16861 tattcaatgt ataagataga atagaacagc taagtaatgt atacagaaaa tgtgtgagat
    16921 ccaaataaag caaggagaca actaaaatac caaaagtaat caagaaaaga cttttgccaa
    16981 tcttaatatg aaaatatttt tcaatggctt gataaatgat atctgttccg cctgtgcttc
    17041 cgccgaatcg aaagacgaga ccgcaaccaa tgccagaaag aatacctgtc atcaaactat
    17101 tgatcagtag actatctaca gcaaaatgta tatgaaggct ctcccataaa tgtaaaaaga
    17161 aaactagcgc aaatgttccg acgatcgtcc ggagaaaagc gtgttttcca aaaagaaaca
    17221 atgagataag caataaaggt aaattaaaaa gcagtgaagt gacagaagga gcgaatccaa
    17281 aaagattatg caaaagcaaa gcaataccag taaccccacc atctgccagc ttagatggaa
    17341 tattgattcc tgtcacagct aatccgtaga ttcctgcgcc taaaatgatt ccgcacaatt
    17401 ctgtaaataa atttaatttt ttcatttcta gcctcttttc ttttgaaaat tttcagcaac
    17461 ttctttatta tactgactta tactcttact tcaagaggaa aatgaaaaaa aattattttt
    17521 ggctgtttct ttttatttat caaataagga gtggaaaaat gcgagcagca gacttattga
    17581 caattcttac aaaatcaccc aaaaaaacag agtatcaagg agtttttttg gctcaagaac
    17641 aaaaactggt gcctgtgaca atggtccaga taaataaagc aaatcaattt atctttttat
    17701 ttgaacacgg caaagcacca cttgctatga aagacatctt tctttttttg atgaagaacc
    17761 gtgagaaagc tgtttattat cagtcaaaag aagaactcat tcctctttat ggagtgaaag
    17821 agatagaaaa taaagtggtg atataagtaa aaataagaaa acaagacgtt tatgatgtca
    17881 aaacaaccat tttttacatt tttggcaaaa aagtgtcatg atacagtata atattcgttg
    17941 aacaggccaa taaaggtgtt ctcccccacg agatttctac tttattggct gtttcttttt
    18001 attaaaataa aagttgtgtg tgtaaaaaag atatagctga ttccaagtga caatttcgta
    18061 tagattttta tggtattcgt agtgaaaaaa cgagaaccaa aaagttgatc cgactttgtt
    18121 gcttggattc tttttttgtc agcatattta gaggtatttt tttctttttt ttgttcgtgc
    18181 tgtaaggtta aggcaagaca agttagttgg gaaaaaggaa agaaatgttt atacttaaag
    18241 aataacagaa aggaaggtcg catgaataat tttatacaaa cattaagtga aagaaaaggc
    18301 gagttgctgg aagctgcatt tcaacatctg tccatttctc tcatttcttt gctgattgca
    18361 gcactgattg ctattccttt agcgatttgg gcagccaatc ataaaaaaat ggcagaagtc
    18421 ttattgcaga tcacgggtgt gctccaaacg attccttcac ttgccttatt aggtttactt
    18481 atcccatttg tagggatcgg aactgttcca gcattgattg cacttgttat ttacgctttg
    18541 cttccgattt tccaaaacac gtatattggt ctagctgaaa ttgacccgtc tattgaagaa
    18601 gcagcggtag catttggtat gtccagaatg agacgcttgc tgaaagtaga gcttcccatc
    18661 gcattacctg tcattatctc aggtatccga actgccttag ttttaatcat cggtacggct
    18721 actttggcag cattgatcgg agcaggtgga ttagggacat ttattttgtt aggtattgac
    18781 cgaaatacac ctgttttgac attgattggt gcgatcagtt ctgccttgct tgcaattata
    18841 ttcagcggac tgattcgctg gttgcagcat aagaaacctc gatattccat cattacttta
    18901 gcagcagtgg tgtgtttatt tggcggcgtt tctttatatc agtctgcctt gtttaaaaat
    18961 gaaaccatca cgattgctgg aaaattagga gcggaacctg aaatcctgat ccaaatgtat
    19021 aaagaattga ttgaagacaa cacaaaaaca gaagttgaat taaaaccgaa ttttggcaag
    19081 acgagttttt tgttcaatgc actggatcaa ggtcaaatcg atatctatcc tgagttcaca
    19141 ggtactgtcc tagaaagctt ggtgaaggta cctgaagcaa tgaaaagtgc tgatttgaca
    19201 aaagaaaaaa catatgacga agcagaagaa ttattgaata agcaattcga tatgacatta
    19261 ttaaaaccaa tggcttacca aaatacgtat gccttggcag tcaagagatc ttttgcgaac
    19321 gaacatcaac ttaaaaagat ttccgatttg gcagctatct ctgatcagat caaagcggga
    19381 ttcacactgg aatttattga tcgttcagat ggatataaag gaatccaaga agcatatggc
    19441 ttgaattttt caagtgtaca aagcatggag ccaagtttgc gttaccaagc aatcaataat
    19501 ggagatgtca atattgtgga tgcctattcc acagatagcc agctcaagga gtacgatcta
    19561 gtgactctag aagatgatcg agggttattc ccagcttatc aaggcgcacc gctgatgaaa
    19621 aaagattttg ctaaagaaca tcctgaaatc gttaaagcac tgaataaatt aagcggaaaa
    19681 ataacagagg atcaaatgat tgatatgaat taccaagtca atgtcgaaaa gaaaaagccg
    19741 acagaagtag cacgaatctt cctgaaaaat gaaggattag taaaggagga agatcagtga
    19801 ctaaattaat cgagttcaaa catgtgcaaa aaaagtatga tggaaagtat gtcatcgatg
    19861 acctaaatct ggcaatcaaa aaaggagaaa tctttgtttt ggttggtcct tccggtagtg
    19921 gaaaaacaac tactttgaaa atgatcaatg gattatctaa accatcagca ggagacatct
    19981 acttcaaagg aaaatcgtta aatgaatata atctgcagaa aatgcggtgg aatatgggat
    20041 atgtgctgca gcaaatcgct ttgttcccga ccatgacagt caagcaaaat attgaagtca
    20101 tccctgaaat gttaggttgg gaaaagcaga aaagagcaga ccgtgtagat gaacttcttc
    20161 aaaaagttgg cttatctcct gatatttatc gagatcgtat gcctagagag ttgtctggag
    20221 gagaacagca acggatcggg atcatccgag ctattgctgc aagcccagat gtgattttga
    20281 tggacgagcc atttagcgca ttagatccga tttcaagaaa ctcattacaa gaattggttt
    20341 tgtccttaca cgaagaattg ggcacgacca tcgttttcgt tacgcacaat atggaagaag
    20401 caatcaagct aggtgatcgt attgcattta tgaaggatgg agaaattatc caatgtgaca
    20461 caccagagca gttattgatg aatcctaaaa acgactacgt tcggcatttc tttgatgaac
    20521 cgaaacagac gaaagagtgg cgagtagaag atttagtcgt caatggatat ttcttaaatg
    20581 aaataccaga aaatgctcgt caacaagttt gctttgatac gcctatgaaa gaagtatatt
    20641 cgcttctttc ggtgtatcca agcattgtga tagtagaaaa gcaaagaagt attgggtcgc
    20701 tgacaagtaa agaaatcttt gcttttttaa gtcgagagga ggaaggaaat gaaaacatat
    20761 gatgcaatca tcatcggttc aggtgtgagc ggcttaagtg ctgcttatgg attgaaagaa
    20821 gcaggtaaaa cagtactagt ggtcgaagaa gatctatggg gcgggacttg tcctaaccgt
    20881 ggatgtgatc ctaaaaaagt cttgttaagt gcagtagagg cacgtaatcg ggtaaaacaa
    20941 ttgagtggaa aaggttttaa cgagatccca actgccaact gggaagaact tcaaaaattc
    21001 aaacgaacat ttacggatcc agtcccagaa agccgaaaaa aacaattagc agaagctgaa
    21061 atcgatcatc tttcaggaac cgctcgtttt ttggatgatt catctattga agtcaacgaa
    21121 gaagtcttcc atgcggatta cttagttttg gcaaccgggc aaagacctac gatcttacca
    21181 gtggagggaa aggaatactt gaaaactagt gcagactttt tatcattgcc tgtactgcct
    21241 aaagaaatca tttttatcgg tggtggttat attgcttttg agctagctac gattgccaat
    21301 gctgcaggga gcaaagtaac cattgtccat cataatcaac ggccattaaa agaatttgag
    21361 gcgtctttgg tagaggaagc cgtccatcaa atggaagcat ctggcattca gtttgctttt
    21421 ggtgttgaaa cccaaaagat catttcagaa ggtactcgct atcgtttagt cggaaaagag
    21481 acggaattag tagcagatat gattttttgt gcgactggga gacagccgaa tacagaatct
    21541 ttagcacttg agcaagcgaa tatcgtcttt gataaacacg gaattgcagt caatgattat
    21601 ctgcaaacga gcaatccaaa aatttttgcg tgtggagata ttgtttcaag gaaaacccca
    21661 aaattgacac cagtcgccac ttttgaagga aattatgtag ctaagcggat aacggatgca
    21721 acaagcgaac ctatcaaata tccgattata cctacgatcg tctacgccag tcctaaactt
    21781 gctgaagtag gagttacaaa aagtcacgcc tcttcgtcag atcaagtggt agagatggat
    21841 cttaccagtt ggttcaccta tcatcgtgtc aatgaaccag ttgctaaggc tgaattgacc
    21901 tttgaccagc aaaattattt gattggtgct gcagtgatta gcgagcaagc agacgaattg
    21961 atcgatgatt tgacactcgt catcaatcaa aaattgacta aaaaagagtt ggatagctat
    22021 atcatggggt atccgacatt ggcaagtgat ctctcctatt tattgaaata atcagcatga
    22081 taactaaagc gcttgttgct ttttccttct tgtgctacac tttaaaaaat gaatacctgt
    22141 ttctggggat gtcgtagacg acatgagagt aagttgtgaa cttagaccca ttgaacctgt
    22201 cagttaatac tggtgaaggg agaaacatag cagacgcttg aagcgggtta ctatgtcgtg
    22261 cccatttcaa gcgttttttg ttattcatat tagaagaaag gaggtaacaa aatgaagaaa
    22321 aatctttatc cagcagtcag tatggcattc attttaatta tttggcagat tgttgtcata
    22381 gtttggaaaa tacctgcctt tattttaccc ggacctgctg ctgtattttc tgcattagcg
    22441 gcagatgcac aagtattact ttcccattct gtcacgacat taaatgaagc agtgcttggt
    22501 ttggtaatag ctagcgttct ttcttttatt actgccttgg caatggatta ttggaagttt
    22561 ttagaaatgt ctttttatcc gttactagtg atttcacaaa tgttgccaat catggtgtta
    22621 gggccattac ttaccctatg gtttggtttc ggtatgcttc caaaagtgat ccttgttgtc
    22681 ttgataagct attttccgat cgtagtttct ttttcggata cattgaggaa agtatcaaaa
    22741 gagcaaatca tctttttgaa aacgatgggg gcagatacag ggaagattta tcgaatatat
    22801 aaaataccag cgggtttatc cggcttcttt tccggtttaa aagtggctgc tacttattgt
    22861 gtcagcggag cagtcgttgg agaatggcta agcgctcaag caggcttagg gtattacatg
    22921 atacgcgtaa aaaacagcta ccagatcgat aaagtttttg ctgcgatcat ttgtgtcatt
    22981 ctattgagtt tattgctgaa tggcgcttgt tcattattga aaaaagggta ttatcgtatg
    23041 ctttatattt tttaagagaa ggggatcaaa tgaaaaaatt atggctttta ttaccgctac
    23101 tgttgctttt atccgcttgt ggcactgcaa aagaaacaag tagcaaacaa gaaataaagg
    23161 atttgaaaaa agtgacactt gttttggatt atgtaccgaa tactaatcat actggcatct
    23221 acctagcaaa agaaaaaggc tattataaag aggctggttt aaatgtccaa atcatcgaac
    23281 caggagataa ttcgacaagt attggtctag taggtgcgga caaagcacaa tttggtgtaa
    23341 gttatcaaga agatgtcact tatgctcacg cagatggaca gaatatacca gtcaaagcaa
    23401 ttgctacagt gatcaaacat aatacttctg gattcgccac cctatcagac agtaacatcc
    23461 attcaccaaa agattttgaa ggaaaaacat atgcaggttg gcaaagtcca agtgaagaag
    23521 ctgtcttaaa agctgtgatg gaaaaagacg gcggggactt ttcaaaactg acaatggtag
    23581 gaagcaatgg agaagggcca gaaagcttag gaaaatcctc agatatccaa tggtactttg
    23641 aaggttggga tatgatcaaa gcaaaagaag cgggtattga actaaactat atcccattga
    23701 aagaattaga tgaacggtta gattactata ctccggtaat tattacgaat gatcaactga
    23761 tcaaaagtga tccagaattg gtacagtctt ttatggatgc cacgaaaaaa ggctatcagg
    23821 aagctattaa ggatccaaat gacagtgcta aactgttgca aaaatatgca aaagaaaatg
    23881 atcggacgtt tttagaagaa tcacaagcat tcttgtcaaa aaattatacg gatgatcctg
    23941 agaattgggg attgatggaa gaaaaagttt ggcgcaatta tacagctttt atgcaggaaa
    24001 acggattgat caagcaagat gtaccttcgc aagaactatt cactaatcag tttataaaat
    24061 aggagtgata gatatgtcaa tccaaatcag ccacttgaat gtagtcattg aaaacctgaa
    24121 aatattagaa gacatccatt tcactatccc taaagatacc tttacatcga ttgtcgcgcc
    24181 aagcggagcc ggtaagtcaa cattgctgaa aactttaaca ggagtccagc ctatgacttc
    24241 tggaacgatc aaagtagacg atcaaaaagt aactggtctc catacagcat ttagttatat
    24301 gccgcaagaa gatatgttac ttccttggct gagcgtctat caaaacgtta cgttatatca
    24361 aaaaatcaat catctaacaa tcaatgaaga acaagtaagg gattatctgg atatctttgg
    24421 gttaaacggc tatgaacatt ttttgccgga acaattatct ggcgggatga agcagcgaac
    24481 ggccttgtta cgaacaatca tgaatccttc ttcttacttg cttcttgatg aaccatttgg
    24541 agcgttagat gctatgacca gagggcagat gcaagactgg atgttgaagc tgcctaaaaa
    24601 agcaaagcgg actactttac ttgtgacgca tgatatcgaa gaagcaattt atttatcaga
    24661 caggattctt gtgctttccg cccgtccggc acatgtgatt gctgagattc aagtgcctga
    24721 aaaacgacgg tcacgtgaat ggctactgca gcaatcagag ttgaagcaaa cgatttatca
    24781 gttattggca ggtgaattag atgttaagtg atgctcattg tcatttagat gggagtcaga
    24841 gtttagcact ccttcagcag gaacacacga ttttgacgat catcaattgt gattcgccag
    24901 aagaatggaa ggaaaacaga cagcttgcag caagtaagac tcaagcctta agttacggta
    24961 tccatccttg gaaagcagat agctatacgt ttgaacaagt tgagcctttt ttgaaaaaag
    25021 caaggatcat cggtgaaata gggttagata atatttggac gaatgtaccg atgactacac
    25081 aaaaaaaagt atttgagcga cagcttgctt ttgcagccat aaatgagaaa cctgttgtac
    25141 tgcacacaaa aggatgcgaa aaagagatct taaattatat ccaagcttac cctaatcact
    25201 atttgatcca ttggtattct tctttggact atcaacaaga ctatattgat ttaggctgtt
    25261 atttcagcat tggtcttgat ttgaaaaaga atccagcagt ctggcaacta gctaaagcag
    25321 tcccaatcga ccacttactt attgaaacgg atggactaca tgcagcagag tgggctttag
    25381 gggggatttt ttcagaaaaa gattatgtct cactgcttga agaacagtta aagacaatcg
    25441 ctgagatcaa agagctatca gttgaagaag ttaaaaaaaa tacttatcaa aacttggaac
    25501 gtttcattag attaggtaaa taaaaaagag atgtgacaca actagtgtcg cacctctttt
    25561 tggctttctt tgtataaaag taaactgttt gattaataaa tgaatcttag taccaaccgt
    25621 ttgcttccca gaatgctttc gccgcttccc aagaaccata acgtgaagta acatattgtt
    25681 ctgctactct ttcttggttt gcagcagagt agtcaccatt taagtaagaa gaatctaatt
    25741 ggtaacgtcc gatgtaacgt ccgttcgttg ctgtgtaaga accgcttgat tctttttgtg
    25801 cgatccattc ttttgcagag cttgtgcttg ctggagtagc agcagtttct gttacagcag
    25861 gagcaggttg tgtttcagca acaggcgctg cttgtttttc cgtttgaacc gtttcttgta
    25921 caacaggttg ttcagcttgg actggtgctt cttgaacggc tgcttgaggt tcagcagctg
    25981 cttgtgcaga gccttctgtt gggattgtta attgttgtcc tgagta
//
LOCUS       DACCCS010000039        25331 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.039, whole genome shotgun sequence.
ACCESSION   DACCCS010000039 DACCCS010000000
VERSION     DACCCS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.039"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(324..893)
                     /locus_tag="IVK82_001705"
     CDS             complement(324..893)
                     /locus_tag="IVK82_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6392771.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="IVK82_001706"
     CDS             complement(967..2256)
                     /locus_tag="IVK82_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392772.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="IVK82_001707"
     CDS             2610..3458
                     /locus_tag="IVK82_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP6392773.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="IVK82_001708"
     CDS             3472..4545
                     /locus_tag="IVK82_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP6392774.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="IVK82_001709"
     CDS             4535..6706
                     /locus_tag="IVK82_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAP6392775.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="IVK82_001710"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="IVK82_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP6392776.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11191)
                     /locus_tag="IVK82_001711"
                     /pseudo
     CDS             complement(9009..11191)
                     /locus_tag="IVK82_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
     gene            complement(11191..11400)
                     /gene="copZ"
                     /locus_tag="IVK82_001712"
     CDS             complement(11191..11400)
                     /gene="copZ"
                     /locus_tag="IVK82_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP6392777.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11413..11853)
                     /locus_tag="IVK82_001713"
     CDS             complement(11413..11853)
                     /locus_tag="IVK82_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP6392778.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11927..12400)
                     /locus_tag="IVK82_001714"
     CDS             complement(11927..12400)
                     /locus_tag="IVK82_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HAP6392779.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(12710..14176)
                     /locus_tag="IVK82_001715"
     CDS             complement(12710..14176)
                     /locus_tag="IVK82_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6392780.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(14487..16571)
                     /gene="cadA"
                     /locus_tag="IVK82_001716"
     CDS             complement(14487..16571)
                     /gene="cadA"
                     /locus_tag="IVK82_001716"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP6392781.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            complement(16713..16901)
                     /locus_tag="IVK82_001717"
                     /pseudo
     CDS             complement(16713..16901)
                     /locus_tag="IVK82_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(17095..17454)
                     /locus_tag="IVK82_001718"
     CDS             complement(17095..17454)
                     /locus_tag="IVK82_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP6392782.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(17484..17825)
                     /locus_tag="IVK82_001719"
     CDS             complement(17484..17825)
                     /locus_tag="IVK82_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392783.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(17822..18496)
                     /locus_tag="IVK82_001720"
     CDS             complement(17822..18496)
                     /locus_tag="IVK82_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6392784.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            complement(19291..20589)
                     /gene="asnS"
                     /locus_tag="IVK82_001721"
     CDS             complement(19291..20589)
                     /gene="asnS"
                     /locus_tag="IVK82_001721"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP6392785.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(20629..21819)
                     /locus_tag="IVK82_001722"
     CDS             complement(20629..21819)
                     /locus_tag="IVK82_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6392786.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(21840..22367)
                     /locus_tag="IVK82_001723"
     CDS             complement(21840..22367)
                     /locus_tag="IVK82_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6392787.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(22414..25203)
                     /locus_tag="IVK82_001724"
     CDS             complement(22414..25203)
                     /locus_tag="IVK82_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP6392788.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDSSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAIYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKEIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
BASE COUNT         7103 a         5394 c         4459 g         8375 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actttccctg ctgttctaat tctaacgcgt gttcttggta ttcatcaaat gataggttca
     9661 attcaattaa tcgtttacgt gtacccgcaa aatagcgtac tccgttaatc gtaccgctga
     9721 ttccagcacc tgggtgagct gtaaaatcaa tcatatcgta tgttgctgct tcttgcagtt
     9781 ttccgtattc cacgattgct ttacctagtg gatgttcaga agcttgttct aaagtataaa
     9841 aaatagatag gacttcttta gaaccgacta gatctgtaac tttgggcttt ccttctgtaa
     9901 tcgtcccagt tttatccaaa acaatgctgt ctaagtgagc agctgcttct aatgcttctc
     9961 cgcctttaat taaaattcca tttcgtgcac caacacccgt tcctaccata atagctgttg
    10021 gagtcgctaa acctaatgca catggacacg caatgactaa tacagagaca ctatggatca
    10081 atgcgagttg ccaatcacca gtgatcaaac ctgtagctat gagcgtgata aacgcgatga
    10141 ctaatacgat cggaacaaaa atccctgaga tccgatctgc gattttttgg attggggctt
    10201 tgctcccttg agcatcttcc accatttgaa tgatttgcgc taatactgtc tgcttgccga
    10261 cttgtgtgac ttccatgtgt agcagaccat tcgtattgat tgtcccgcca aaaagcgtgt
    10321 catccggatt cttttctaca ggaagatttt caccagtaag catactttca tctatcgcac
    10381 tgcttcctga aataattcga ccatctgctg gaatctgctc accaggacgg atgactagtt
    10441 gatcaccaac gactacttct tcaatcgcta acgtttcttc tttcccgttt cttatcactt
    10501 gggcggtttt tgtttgaaga gacatcaatt gtttgattgc attacctgtt ttcgtttttg
    10561 ctgtatgttc caaatatttg ccgagcaaaa tcagcgtgat gatcatactg ctgctttcaa
    10621 aataaagatc ttgggggtgt ccacggaaaa atccgttgta aatgctcaat gcaaaggcag
    10681 ccgatgttcc aattgctacc aatacatcca tatttggtgc tttcgacttg attgcatggt
    10741 acgcaccttt ataaaaacga gcaccgacat aaaattggac aggagcagac agcactaact
    10801 ggacgattgg aaaatgaaaa aagtgaacaa tcgccgcatg gcttcctaac atcatagcaa
    10861 tcattgacaa catcaacggc aaagtcaaaa ccgtactcaa tataagatcg aatagcattt
    10921 ttttcaaata cgcttgcttt tcttctgcaa ttttttgttt gtgggcttca tcgtacaaaa
    10981 ttgcaccata gccgatgttt tctacgcttt ggattaattt ttcagtggtc gtcccttcat
    11041 atttgacact tgctttttca gtggccaagt tgactgtggc attgaccaca ccaggttgtc
    11101 cgttcagctc tttttcgatc ctagcagaac aatttgcaca agtcatacct gtaatgacga
    11161 acgtatccat tttggtttct gctgttgcca tcaaatcacc tctgctttat aacccaatcc
    11221 attgattgct tgacaaatct tttcggattg aacttttgtt tcatcaaact tgaccgtacc
    11281 gtttgctttt ttcaaattta ctttcacttt ttgcacgccg tcaagtgcgc ttactgtttc
    11341 ttctactctt gctgcacaat gattgcagct cataccttcg attgaaaatt gttgtttcat
    11401 tttttcttcc tcctattgat gttgtttaca ttcacattga cctgggatac agttacattc
    11461 gatcgtatct actggttgct tttgttccag ttgtttcaaa atctcattga tatcttcttg
    11521 agtcaaagtg gcttcttcta ctagatccgc aatcgtctct ccaatccttt ttgcacagat
    11581 atgggaaaaa agattttccg tcgcactgcg aactgtttcc atctccgaga cagctggatg
    11641 ataaatgaat ttttttcctt cttgttctgt ccagatcact tcttttttta ccaaacgacc
    11701 cagcaaagtt ttgatcgttg ctggtttcca atccatcgaa tcagctaaga tctgcgtgat
    11761 ttcttgggct gtcgtctgtc caagtgtcca aataacacgc atgatctccc actctgaatc
    11821 actaatcttt actggcgttt tgactttttc catttatttc acacctttcg tttacaaatg
    11881 taatcattaa cttatgaata cagattacac ttgtaaacaa aaactgtcaa ttatttttgc
    11941 tctctatttt caatttagtt aaaaattctt gtttctctcg tcttgtttta aggatgatca
    12001 gtttatttct tgcattagct acttggatca gctgctgtat ttttggttca ttgtttcctt
    12061 caaaatgcca gataaaagat aaaaattcta agtatttcct gtttagtttc tccgaaaatg
    12121 attcaggcat atctggccga gattttttgt ttatctttct ttttagagaa cgccaaacta
    12181 ctcggtagat ggcgagataa cgcggcactt taagccaaat gatcttatct gcttctttaa
    12241 tcctttcctc taacgtagaa gtataatttc cttcaacgat ccatgacgga ttagatacca
    12301 taaacgattg ctgttcttgc aaaaaccact tttttgcctg aatgcggaaa gccggtgatt
    12361 tctttgatct tccttgcgag ggacgatttt cccgacccac tttgtccaat cacgatgatc
    12421 ttcattcttc tccctcactt ctctttggaa tttggttcga tcataccact tataacgccg
    12481 caaaaaaaca agggactgtg acaaaagtcg tgtcaaagtc ccttgtttcg aataaatggt
    12541 gttcaatcag ctgaccttca gcattagctc aaaaaacggc aaaatatgag gagctatttt
    12601 tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct gattcacggt
    12661 gttccagaag tagcttctgt cacaacctct tgtaacaagt atttctattt taaggctgaa
    12721 ccactgtttc tggttcagta atttctttgt ttttgtctcg acgagcaatt gctggcaaga
    12781 tcatacctaa taagatcaag acaattggtg tgatgatatt cgtgaataat tggaaccacc
    12841 atgctttagg atcagcagta taatctactt ttggcaccat tcctaggata catgcaaacg
    12901 cagtgaaggc aaagcaccaa gcaccaaaga taaaaccgat ttttggattt ttaacaaatt
    12961 tatattcaga attgaatttc ttataagctt tattcaacat catatatgcc aagaacaccc
    13021 ataggtaacg cattggcatg acaactgagt tcaagttcgt caaccatttt actaattcat
    13081 tcatatcttt gataccaaag atcggcaaca aaataatgat tcctactagt atacctgtca
    13141 gaagataacc attcaccaat gtacctttac gattacgttt acgtagccag tttggtacaa
    13201 attctggatc cgcatctgct aacaagattt ttagtggtgc atcaattgaa aaggctaatg
    13261 cggagatttg tccaatcatg tttgtcaatg cgtaaatgat catcaatgag ttaccgatac
    13321 catagtaatt acctaaaatt tgaaaagcac tgtatgcccc atttgccatc aagtctttag
    13381 ggatattatc gctagcaaac agcataccca ttgcgactga gcctaataca gcacaaatcc
    13441 ctaccattgc tgctaagaag atcatccctt taggaaattc ctttgcagga tttcttgtag
    13501 agttgacata aggggatatt ttttccgccc ctccaactgc aaatactagc atcgaaacgg
    13561 ttgtgaaata actgaaatca aatgttggaa tatatgtttt cacttgattc atatgtggtg
    13621 tggcaaattc catcgttgga ttgatgaacg gtgccgttac agctaaaaga ataaataaga
    13681 tcgacatgac gaacatcgct gttccggcca gtccgccaat cactttcaaa gtagaaagtc
    13741 cttttgttga taaatataaa aaggcaagaa atataataag tgaaataact gctaccattt
    13801 gtactgacat cgtatttacg atatttccat ttccttggac tgcccaacca ccagcaatca
    13861 gaattgcttg aggcttttgt gccaaataag gaatatggac gacccagtaa gtccaagcgg
    13921 catagtatgc cagtcgttta ttagatgtcg tattttcgac ccatgaactg acgcctcctt
    13981 tactatcctt gaaggctgat cctaattgtc caacgatcaa tgcgtatgga ataaaataaa
    14041 tggccaaaat caatagccaa gaggtgacaa ctgatactcc ttgctgagca tagttattaa
    14101 ctacattccc catgccccaa accatgttaa atgcaattaa cgcaacagta aaccagcgta
    14161 attgtttgtt gttcattcgt tctctccttt atgataaaaa tttgttatcc agatgtaatc
    14221 atattcaatt tttaacaaac ttacaagaca gtgttatttt atgtgtgtat tcttttattt
    14281 aatacacatc gttttttcat attttttcca tcaaaaaaat aagttatttt cttttatttc
    14341 attgacctta ttgaatttca agctattttg aattttctgc aaaattaaaa acattagtta
    14401 ttttataatc ctatctattt tctttacatt tttcattaag aaaaggatat aaaaaaaaga
    14461 ggacgttcat tcgtcctctt cattgatcaa cgtttatttt ttacatgcaa gcttcgcatg
    14521 gcattcaaaa cagctaatac tgtcacccca acatctgcaa atactgcatc tcccattgat
    14581 gcaaaaccaa aagcacctaa ggaaagtacc aaaattttta tcccaatagc aaagacaatg
    14641 ttctgtttga cgattttcaa tgttttcttt gctaaatgga ttgcttctgc aatcttcgat
    14701 ggttgatcat tcatgatgac tacatctgct gcttcaattg cagcatcgct tcccaatcca
    14761 cccattgcga ttcctacatc tgctcttgct agcaccggtg catcgttgat tccatctccg
    14821 acaaatgcgg tctttttatt gttactttgt aagatttcct ctaaccgctg cactttatct
    14881 tctggaagca attcgctata tatcttgtcc acgcctactt gcttcccgat atgatcagca
    14941 atttttttcg agtcgcccgt caacatgact gtgtttttca caccagcctg ttttaattga
    15001 accaatgcgt ctacggcatc ttctttcaag gtatctgcaa taacaagata accactgtaa
    15061 ctttgatcga tcgctagata aagcaatgta ccgatttctt gacttgaagg aaaagagatt
    15121 tcaaatcttt ccatcaattt gtgattacct actaatactt gtttgccatc aacttcagct
    15181 aagatccctt cacctgcaaa ttcctggata ttagctgttg gtaaaagcgg acctgcgtag
    15241 ccttctaaaa cagattgagc aatcggatgt gtagaaaact gttctgcact tgcaacatat
    15301 tgcataaatt tctttggatc aattcctgtt ggatcgatcg tctggatcga aaagtcacct
    15361 ttcgttaatg tgccagtttt atcgaatacc actgtctcag tttgtgcaag caactccaag
    15421 taatttgaac cttttactaa tacaccgatt ttacttgctc ctccgatacc gccaaagaaa
    15481 ctaagtggca cggaaatcac taatgcacat ggacatgaga tcactaaaaa tgtcaatgct
    15541 cgatagatcc attcagaaaa cgccgctcta gaaacaacaa gtggaggtaa aacagctaat
    15601 aaaacggcca atcctacaac gactggtgtg taatagcgcg cgaatttagt aatgaaattt
    15661 tcagctggtg cttttttact gctggcattt tctactaatt ctaataattt gctgatagta
    15721 gaattttcat aagatttttc tacttgaact aaaaatggtt tatttttatt gataaatccg
    15781 cttaatactg tttcaccaac atatacagat ttagggactg attctcctgt aagtgctgac
    15841 gtatcgacta atgattctcc ttcgatgatc gttccatcta acggtacacg ttcaccaggc
    15901 ttcacaagta caaactgacc gatcagcacg tcttcagggt tcacggtcat caatccttcc
    15961 gccgtctgca cgtgagctgt ttctggacgg atcgccatca attcgcgaat cgattttctt
    16021 gaatgactga ctgccaatcc ttggaaatac tcgccgattt gataaaatag catcactgct
    16081 acggcttctg gatattcacc gatcaacata gcgccaatcg tagcaacaga cataagaaaa
    16141 ttctcatcaa agatttttcc tttgacgata ttcatcactg ccttcttcac tacatcatat
    16201 cctatcagca gataaatcaa taaataccct atcaagcgaa ttggttcatt aaaatcaaat
    16261 aatagcaata aagcgatacc tgcaagggaa aataaaatcc ggaacagcgt atttctcttt
    16321 ttttcatttc catgttcaga tactttttcc ttatctattt ctgttacttt gacatctggt
    16381 tctaattcct tcactacttt ttttgtttct tgttcaatcg tagccaaatg atcttcctct
    16441 gcgtcaatcg ttagttttcc agaagtgaag tttaccgttg cttccttgac tccattgatt
    16501 ttctgaactc ctttttcgat tttcattgca cagttcgcac aatctaaacc ttctaatctg
    16561 taacttttca ttttaatgac ctcctcgcct caacatatga atatctcttc ataactaatt
    16621 ataaatgaat ggttattcat atgtcaaggt atccgaaaga aaaaatgaaa aacatctgca
    16681 tcggaaaatt tcttccatac agatgttttg gcttattctt tctttttctc ttcgatcatt
    16741 gccactgctc ttatccaacc cgcacttgcc ttcttgatct tgacaggata gcaactaagt
    16801 ttgaagcctg ttgctggcaa tttgtctaaa ttcattaatt tttcgatagg acaatatctt
    16861 ttttcaatcc ctgcaaaatg tccttcccag atatacgaag ctccactacg ctttcaaaaa
    16921 tatagaaaga gttctttaat gaaaattatt tcaactttcc ataaacagta accccatata
    16981 aataaaagac gtaaaagtca catagaagaa cttatacagg aaaacgcata gagtcaggtg
    17041 aaatacatgt atcccacctg gctctatgct ttatctaacc tttattattc tcttttatac
    17101 aatttctgca ccgaatggca tcaacgccaa tcgagcaagt ttgaaagatt gcttggcaaa
    17161 aggtatccct atgatcgtga tacatagaag gatgccgctg acgacatgtg cgagagcaat
    17221 tggtaggcct gaaacgatca gccagatgat attaacgatc aatgacatgc cagactggtg
    17281 ataaattact tctttgccaa aaggcgcaaa agaaagggag gcgatcttaa aacattgcat
    17341 accgattggg atacctatga tcgtgataca ccaaagtatc cccgctgcta accagctaag
    17401 accaccgatt agtccgccga atataaacca gatgacattt cctaaacaac tcattgtatt
    17461 tctccttcta ttactgtttt tgattaaaac tgggcaaaaa ttgattttat tcttcttttt
    17521 ttagcttcca gcatcaagta ttctgcaaat tttttttgga tttcttccgt attttctttt
    17581 gttactaatg tgtgttggat tagttttcct gatggtcgct gatccaatcg ttcttctgac
    17641 tgttgcacgt ccgcttgcgc aaatccgata atccgttttt cttttgttac agcaacgagc
    17701 aatagtttat tcttatcatt taggtcagct gccgtttttg cttttacgaa tgattctgga
    17761 tgatcagtaa tcagctgatt gatttgaggt aaatcttctt ttactgccca ccttatttcc
    17821 atcatttttc acttttcttt cgctcatttt tatctccact aaaaaaatcg actgcgacaa
    17881 ttgcatctat ccagctttgt gcaatgatct catgtccgcc aatcgttggg tggaccccat
    17941 cttctcttgt atatttcgaa aaaccataag caatccctaa agcattgaat ataccatcta
    18001 aaggaaccag caatgcgcca tattccttag caagtttacg aacaatttgg attttagggt
    18061 ctaagtcttt acgccagcca tgacggtcgt cggttatagg aagtacaaac ggctccatca
    18121 agatgatgtt gcagatttcc gctgtttgaa gttcatttag taattttcga taaacggatt
    18181 caaattgctg agcagatttc tcagttccga atatagcctg atcgctagta ttatgccagg
    18241 tatcattgat tccgatcaaa atcgatacat agtcaggttg taaagctaaa caatcttctt
    18301 gccagcgtac ttgcagatct gcaattttgt ctcctcctat cccacggttc atgcattcaa
    18361 attccatcgt tggatattgg gagattagtt ttccggcaat caaaagtgga taacctttac
    18421 ctagatcagt tgcttctgct cggtatcttc ctgcgtctgt aatgctatca ccgataaata
    18481 atatacgttt tttcatttgt caaaaactcc tttttgaata aaacgatgat tttcttcctt
    18541 atattatccc ataagcccta tcaagacgaa agagaaaata tatgtataaa aaatgacacc
    18601 agcctagttc cttcaaacaa ttgaatgaac gctcatcaag tgtctagttt tttatgctat
    18661 ttttttctta acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagagatcga
    18721 tcacacccaa gcttccgtca atcagaccac tgatgacgcc atcgaaacat atatttatag
    18781 gctcatctaa gacttctagt aaatgtccct ttactaatat ctcttctaga ttgcttggga
    18841 tagattggtc ataactttat ctggtccgat gatttcaagg gtgaattgaa cttacatatg
    18901 gattattgca tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt
    18961 ttcaggtgct agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat
    19021 ttgaattacc ttctttgtaa atgtttgatt gattgcgatt tcgttataga accttgtgaa
    19081 tcaaatagca atttcttttg ctttcttctc ttgtctgctg gaaacttcca ttatcccgta
    19141 tttaaaaaaa agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg
    19201 gccacaagtc acaatagtca tcaaacatga aaaactgttt tttgaaatta ctccttgtga
    19261 ctcggagctg aacggcaggc gcacaacctc ttatggataa attcggttca acaaacgtgg
    19321 gaatggaata gcttcacgta catggtcgat accagcaatc caagtgactg ttcgttcaag
    19381 acctagacca aaaccagagt gcggaacaga accgtattta cgtagatcaa gataccatga
    19441 atattctttt tcatccaaac cgtgattacg gatttgttct agcaagaaat catagtccgt
    19501 tgcccgttcg cttccgccaa taatttcacc gtaaccttct ggtgcgatca tatctgcaca
    19561 aattaccacg tcttctcttg ttggatgtgg tttcatgtag aatggcttga tggcttttgg
    19621 atagtttagg ataaagacag gctgttcgaa tgaattcgca atgaacgttt catgaggtga
    19681 accaaagtct tcgccccatt cgatgtcatc aaatccattt ttcttcagta attcgattgc
    19741 atcatcgtaa gtaatacgag ggaatggcaa ttttgtataa gtttttaaga cttctttgtc
    19801 tcgtcctagt acatctaatg cgtattcgca atgatctaaa acattttgta ctaagaatgc
    19861 tacatattgt tcctgtactt ctaaactttc ttcttgatga gtgaaagcca tttctggttc
    19921 gatcatccag aattcgatca aatgacgtcg tgtttttgat ttttcagcac ggaaagtcgg
    19981 accgaatgaa aagacttttc cgaaagccat agcagcagct tccatgtata attggccact
    20041 ttgagataaa taagcttttt gatcaaagta atttgtttcg aatagatccg tagtgccttc
    20101 tggtgcagaa ccagtcaaga tcggcggatc aattttgaca aaatgatgtt gattaaagaa
    20161 ttcatacgtt gcacgaatga tttcatttct gatctgcata atcgcatgtt gtcttgaaga
    20221 acgcagccat aaatgacgat gatccatcaa gaagtctgtt ccgtgttctt ttggtgtgat
    20281 cggataatcg tggctctcgc ttaccacttc cacatttgta attccgattt cataaccaaa
    20341 ttttgaacga ctgtcttcac ggatttctcc agttactaac acggatgttt cttggtgtag
    20401 atttttagct gtttggaaaa cttcttccga cacttcgctt tttacaacta caccttggaa
    20461 aaaggctgtt ccgtctctta attgtaaaaa tgtgattttt ccgcttgatc gcttatttgc
    20521 gacccaagcg ccgattgtta ctgtttctcc cacatggttt ttcgaatcga tgatttgaat
    20581 ttgttccacg tatgactctc cttttatatt ccttgaattt cttcaggatt aattttgact
    20641 tttactctca ataaattgct tgattctgcg taccgcttct tctaacgtag cgcgatcagt
    20701 agcataactc atgcggacat tttctggtgc cccaaagcct tcgccagtca caagcgctac
    20761 ccctgtttct tctaaaagat cttctaccca ttgtgtgaca ttttcatatt tacacatgcg
    20821 tagcgtttct tttacattag gaaaaagata gaaagctcct tgcggctttt caagagaaac
    20881 acctggcagt tctgccacaa gcggatataa cgtattcagg cgttcttcaa aggcgttccg
    20941 catttcttct actgtatctt gctcaccttt caatgcttca acagctgcat attgactgac
    21001 tgctgtcgga ttactggttg actgggaagc aacagcaatc attccattga tgatctcaga
    21061 attccccacc gcataaccaa tgcgccagcc tgtcatcgca tatgttttag acacgccatt
    21121 gatgatgatg gtctgatttt tgattgcttc tgaaatcgtt gcaattggag taaactcatt
    21181 gccattataa actaaacgac cataaatatc atcagcaact atcaaaatat cattttttac
    21241 tgcccattca ccgattgcct gaagttcatt cttgctataa atcattccag tcggattcga
    21301 tggagaattg atgattacgg cttttgtttt ttctgaacgt gcttgttcta gctgttcaac
    21361 agtaactttc cagctattgg attcttcccc tttcacaaag acaggaagtc cttctgccag
    21421 ttttacttgc tcaccgtaac tgacccagta aggcacagga atgatgactt catcttgcgg
    21481 gtcaaggatc gtttggaaaa gggtatacaa agcaaatttc gctccgtctg ttacgactgt
    21541 ttgtgaaggt tcatagacaa gtccatagtt tttcttcaga taatcaacga ttgcctgtcg
    21601 aagctctggg attcctgctg ttggtgtata atagctagct ttcccattgc ggattgcttc
    21661 gattgctgct tcttggatgt tttctggtgt cgcaaaatcc ggttctccca cagtcaaact
    21721 cagcacatct ctgccttttg cttttaatgc cttggccttt gccgcagctg ccaaagtgac
    21781 agacggttct aatcgttttg ttctttttga taactccatc ccattcattc ctttaccttt
    21841 taaattccct tgattgtctt gacttcatct ccagattcaa agccaatcag gtaataattc
    21901 agtttcccat ttgtttcttt tcctactact tcccacacag gctgatcatc atacatccct
    21961 aaagcagctt tttcgatttg gatttcagga tgggccgcag caattgtctg ttttgcttcg
    22021 ttttcagtca gtccctttgt ttgatccaat acttttactt tttcaccaga tttaggaatg
    22081 ataacggcga tttcttttcc tttatcattt gtacctgtca gtgaaaagta tgtcttcttt
    22141 cgagtaaacc aataaaaatg atcggtttcc tcaagatttg catatttctt tgcgatttct
    22201 actgcttctt tcttcgcctg caccgccgga cgactggaac gcaagtaaaa aatcgagctg
    22261 atcacgatga ccgctaataa aaatacgatc aagccaagta acacgcgatt aatcgttttt
    22321 tcctttgtct gttttttctt caagggtcgt cgctccttta tcttcattcc ttttcattat
    22381 agcagatttt aattatgaaa aaagacagct cctctaactt ccttcttcat ttttctttaa
    22441 gaattcatgg atatcctgat agatttcctc catagctagc tctttgatcg gtactttttt
    22501 cggtaatgca tggatgatcc gtgcgccata ttttgcagtg actagtcttc gatccaaaat
    22561 aagcaatacg ccacgatctg tctccgtcct tatcaagcga cctaatgctt ggcgtaactt
    22621 caaagctgct ttaggtacgg attcctggta aaaaggattg atcccctctg attctaaata
    22681 agcgttccta gctttgacca tcgggcgaag cggattatcg aagggtaaac gtgtgactac
    22741 aacgatctgc aaagcatctc caggcaaatc tacaccttcc caaaagctgt cagcgccaaa
    22801 caatacgctt ttttctgaca aaatgaagcg tttcaaaagc ttctcccggc ttccaccaaa
    22861 cccttgagct agtatttctc gtcctttttc tagcatagac agatgcattt tctgatagac
    22921 cttctgcaaa acatcatgag aagtgaataa cacaagtatt ggccgatctt cttgttcgat
    22981 cagacctgta agtgctgtct ctaaataatc gatgtatgct tgattgctga cttgatggac
    23041 ggcaggtgcc tcttttggca catagatcct tgcctgtttc tcataatcat aaggagaggc
    23101 aatcacttta agaggtgctg cttcaatgcc aagcctctta gagagatagt gtcgattagg
    23161 gccgatcttc agcgttcctc caatataaag aatacgatca tatcgaggat accaaacagt
    23221 cgacggcaac atagccgctt ggaaatcatg gagatgaaaa atcatctgct gcgctgtgtt
    23281 cccaggaacg aaccaatgga tcaaatgatc tcgccagtct tccagccatt gatccatcag
    23341 caagtactgc ttgtctaatt gatcaaataa gctatataaa cttgctaaat cgatccgcat
    23401 ctggtttgtc cagctttctt ggataatctc aaaagtcaga cgcatttgtg actgtagctg
    23461 ctgaatctcc tgataaaaaa gctgcaggtg atcaatggct gtcccagatt ccagtggcag
    23521 ctgatccagc atctcttttg tcatcatgac ttcttctgga tactgtccgt tgggttgctt
    23581 gccgctcact aaactcatta atccttctgc catacaaatc tgtgcttcgc atagcgcaga
    23641 aagttcttct cgataaatcg ataaccagcg taaggcttca tgttctttcg gtaaatagct
    23701 ttgtattcgg tcaaacagct gatttggttc ttctaattgc tggatttgtt tatggaaata
    23761 gttaatactt aatcgtttgt catttacttt ttcagcgatt tccggcaaat gatgtacttc
    23821 atcgatcaac aaaaatggac tagctggaag caatggttct tttcgctgtg tttcttgtac
    23881 taaaaacgca tgattgacaa tcaaaaaatt actttgcctg attttctttt gcaagaaacg
    23941 aacaaagtct tgttcgtaaa acgactgatt tcttgctaaa aacccagtac ctctgtgcgc
    24001 gatatctgca aagaatgcat ggttcaagtt tgttaaatgt aattcatcca aatcaccggt
    24061 cttcgtttgc gtcagccaaa ctaagaccgc catttgatac aaagcatatt gtttttgttc
    24121 tggtggttcc acaagtgttc ctttaaatcg ttgcaggtcg atatagtggc gataactttt
    24181 gataattaca ggttgtatgg gctgatctag caattgattc agcaatggaa tttctttttc
    24241 tatgatctgc tgctgcaaaa ctagcgaaac ggtactgatg acagcgggtt tttcaggtgt
    24301 agccaaataa ctcaatggca acagataacc gattgtttta cccattcctg tagctgcttc
    24361 gatcatcaaa tcttttgatt catcttttgt gaaatggtcg tagatcgcat tcatcagtct
    24421 gttctgttct tttcgataca tcagtgtttt accaaacatc ttttcttttg cttgtttttt
    24481 tcttggatat gtccgctcgc cgtaatggac ggatgtgaat agttccactt cttttttctg
    24541 caaagctagc ccatcgacga tttctaatga ggaatcaagt ggctctggtt tttctttcat
    24601 ttcttcaatc acatgataaa tgaaacgact cgtatccata cctgtgtgtc tacttaatcg
    24661 cgcaatctgc tctaacgtga tgattggcaa ttttctcatc cgttcttcga tcagcaaaag
    24721 aagttgacct gtcacttcgg catcactatc tgcctgatga ggattctcat gaataaatcc
    24781 aaggctttct gacaaatcag ctaagcgaaa gcttggttcc gtcggcaaga aaatttgtgc
    24841 aagttctact gtatcgatgc ctggtatctt caaactagga agtccgcatc gcatgagttc
    24901 atggttcaaa aaattataat caaaataaat attatgcgcg acaaatatgg tgtctgccaa
    24961 aagattgtaa atcgtttggg cgacatcttc gaaataaggc gctttttgga cgcgttgatt
    25021 cgtgatccct gttaaatgct ggatttgttt tgaaatcttc cgccctggat gaatatcgat
    25081 cgaaaaacgt gaagtgatcc gacctgattc aatcatgacg cagccaaatt ggatgatccg
    25141 atcttctttt ggattcgtcc cagtcgtttc gatatcaacg actgcataag ttcttggttt
    25201 caaaatatct tcctcttctc ctgcctctaa tgttcttttg aaattatact acaaagtgca
    25261 gaaaaatact ctcatcgctt tttaaagaaa atagacaaaa acgcccagac acgcaaatgt
    25321 ctgggcgttt a
//
LOCUS       DACCCS010000040        23657 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.041, whole genome shotgun sequence.
ACCESSION   DACCCS010000040 DACCCS010000000
VERSION     DACCCS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.041"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            255..530
                     /locus_tag="IVK82_001725"
     CDS             255..530
                     /locus_tag="IVK82_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392789.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            543..2621
                     /locus_tag="IVK82_001726"
     CDS             543..2621
                     /locus_tag="IVK82_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP6392790.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2781..4667
                     /locus_tag="IVK82_001727"
     CDS             2781..4667
                     /locus_tag="IVK82_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6392791.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="IVK82_001728"
     CDS             4679..5626
                     /locus_tag="IVK82_001728"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP6392792.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="IVK82_001729"
     CDS             5646..6173
                     /locus_tag="IVK82_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6392793.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="IVK82_001730"
     CDS             complement(6236..6889)
                     /locus_tag="IVK82_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP6392794.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="IVK82_001731"
     CDS             7021..7863
                     /locus_tag="IVK82_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6392795.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="IVK82_001732"
     CDS             8021..8917
                     /locus_tag="IVK82_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6392796.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="IVK82_001733"
     CDS             8920..9633
                     /locus_tag="IVK82_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP6392797.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="IVK82_001734"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="IVK82_001734"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6392798.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="IVK82_001735"
     CDS             10168..10395
                     /locus_tag="IVK82_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP6392799.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="IVK82_001736"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="IVK82_001736"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6392800.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="IVK82_001737"
     CDS             complement(11181..11723)
                     /locus_tag="IVK82_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP6392801.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="IVK82_001738"
     CDS             complement(11960..12358)
                     /locus_tag="IVK82_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP6392802.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="IVK82_001739"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="IVK82_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP6392803.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="IVK82_001740"
     CDS             13471..14238
                     /locus_tag="IVK82_001740"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP6392804.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="IVK82_001741"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="IVK82_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP6392805.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="IVK82_001742"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="IVK82_001742"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP6392806.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="IVK82_001743"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="IVK82_001743"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP6392807.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18894..19799
                     /gene="xerC"
                     /locus_tag="IVK82_001744"
     CDS             18894..19799
                     /gene="xerC"
                     /locus_tag="IVK82_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP6392808.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19836..20384
                     /gene="hslV"
                     /locus_tag="IVK82_001745"
     CDS             19836..20384
                     /gene="hslV"
                     /locus_tag="IVK82_001745"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP6392809.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20398..21798
                     /gene="hslU"
                     /locus_tag="IVK82_001746"
     CDS             20398..21798
                     /gene="hslU"
                     /locus_tag="IVK82_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP6392810.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21816..22607
                     /gene="codY"
                     /locus_tag="IVK82_001747"
     CDS             21816..22607
                     /gene="codY"
                     /locus_tag="IVK82_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP6392811.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22689..23564
                     /locus_tag="IVK82_001748"
     CDS             22689..23564
                     /locus_tag="IVK82_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP6392812.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8192 a         4079 c         4983 g         6403 t
ORIGIN      
        1 tttgtcgcag ccccattttt ttgttgtgat aaaagaacaa gaaaggcagg aatgggaaag
       61 taaacatcct tttgtcagaa acttttatct ttcttaagtt ttacttgata tctgatacta
      121 tttggaatag aatgaggcgt tgaaaaattt atggaaattt ctaactagca ataaacgatg
      181 aaacaatgaa atatttttca tccaaaaaga actttgctat aatgatggaa gtttgtgtat
      241 aggagatctg gaaaatgaga gagagttttg aacaacaaaa aaaactgttg catgatcgct
      301 atggcgccct ttcgatggat gaccgccgtc aaatactttg taagttgcgt aaaagaaata
      361 tattgatgta tcgtcagttg gaacgtctga aacatgatct gcttcgtttg gagtcaaaac
      421 gtgtacagtg tgaattagag gggaaccaaa cacaggttga agtagtcgaa accaaaattt
      481 taaagaaaaa agaacaattt ttaaaaatgc ttacacaaaa taaaaaataa aggggatgga
      541 cgatggaatt tgctgagtta gttattgtat ttgcagtaac gatcacctta tcgaacatag
      601 ctagccgcat tttgccaatg attccagctc cactgatcca aattttcctt ggagtggtat
      661 taggtctgac agagtgggga caatcaatcg attttgaacc tgaattgttc ttagttatga
      721 ttattgcacc attgttattt agagaaggag aaaaagcaga tatttcttcg attctcaaaa
      781 atttcgggac aattttgttc ttggcatttg gaggagtcat tttgaccctt gtcggggtag
      841 gtgcgacgtt atcattcctg ttgccaagtg tcccacttgc tgcctgcttt gcttttggag
      901 cagcgttagg tccgacagat gcggtagcag tcagttctct ttctggacga gtgaatatcc
      961 ctaaaaaggc gatgcatatt ttggaaggag aaggtctttt gaatgatgca tctggtgtta
     1021 ctgccttcca gtttgctctt ggtgcgctga tcaccggatc tttttcagca gtaaacgcgg
     1081 ggatgagtct agtagtttct agtattgggg gagctttgat cggctttttg cttgtctggt
     1141 tcaaacaaaa aataattcat ttgatcgaaa aagcatcagc acaagatgtc acagggtatc
     1201 tgctgatcga acttttgctt ccttttctag cctatgtatt ggcagaggca tttgatgtat
     1261 ctggaatcat tgctgcagtg gcagcaggta ttctccaagc ttctggtttt aggaaaatct
     1321 ctgtgtttga tgcagaattg tcgagcttgt cccacagtac atggacaact attgctttta
     1381 cactgaatgc attagtattt atatttttag gaatcgaatt gacgcaagtg ttctcgccag
     1441 tatggggaga cggtttatat ccaaacggtc ttttattagc gattatcgta ttaatctctg
     1501 tcatgctttt tgtgatcaga ttcatttcta tcagtctttt ttatgtattc aaagatggca
     1561 gtaaaaagtt caaaaaacag ttgaacgaga tcttgatctt aacatttggc ggtgtaaagg
     1621 gaacagtcag tttggctacg atttttattt tgccattttc aatcaataat atgatgtttt
     1681 accagcggtc tttgcttttg tttctgacgg cgggtgtcat cttagtgact ttggtcatag
     1741 ggatcattgt cttgcctatg ctgacggaaa cagaggaagc gaaaagcacg gatttgaatg
     1801 ctttgatgat tttagaagag gtcgttgaag ttttgcgaaa agaaataaag gaaattgatc
     1861 gtaatacaaa agaattttta gccactgaag cagtgataga aaattatcaa gaacggatac
     1921 gtgatctgta cttggaagat ttaaccgatg atgaaaagca agaagtacaa gaaatacaag
     1981 cactgatttt gtcgatcgaa cgggatggat tggatgaaag ttatcgttcg ggcaagcttt
     2041 cttctaatgg ttatcgtttt tactcccgtt ttctttctcg attcgaacat tctatcacaa
     2101 gccagatttt gtcttttatc ggcttttggt tcattgttgt ccgtaggtta atgcgaatcg
     2161 ttctccatcc gaaaatgttt tggcaaagaa gatatgctga tcgtcagaca tttattagtg
     2221 atgaagatat ccaagaaatc agagagacat atcaaaaaaa tactcagttg attattgaaa
     2281 gtttagataa cttgactgat atttatgatg aaacgatggt tcaatttttt atccagcaac
     2341 gcaaacttga aggaataaaa atgaactcgg ggaatttgat ttcttcgtgg atgatccagc
     2401 aagatagtct gtttacgaaa aaaatgctaa gaggttatta tcttgaaaga aaaatcattg
     2461 atgaatacga agttgctgaa aaaatcacga cgttttctgc gaacagctat cgcaggaata
     2521 tcaatttgtt agaatcttat acgatgaaca aaccatctga taacttttct ttccgatttg
     2581 cgtttcgagc aaagtcaaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcattttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacaccggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt cttgcgtttt tcttaagaat ttctcaaaca aaatgtgagg
    18781 aattgcgcca attgtgaaca aacttagaaa tattggttaa attctgacaa tttatttgtc
    18841 tttttataaa cgctgtgcta aattattctc tgggacaaaa ggaaggtgag aaaatgacag
    18901 aagccacgtg ggaacaaaaa tttttgcgtt atttgattgt cgaaagagga tattcagaaa
    18961 aaacgaaaga agcatatgaa gaagacattc tccatttcaa acattttttg gaggaatctg
    19021 gagacgctga tctgctgaag gcggaatatt ttacggttcg tgtctatttg agtgcattag
    19081 ctgatcatcg tcctagctat agcagaaatt ctatcagtcg caagatagcc agcctacgtt
    19141 cgttttatca gtatcttctc aaagaagaag tgataaaaga aaatccattt tcttacgttc
    19201 atctcaaaaa gaaaaatttg agactgccac gtttttttta tgaaaatgaa atgcaagcac
    19261 tttttgacag cgtagcagga gatagtcctt tggatttgag gaaccgcgct ttactggaag
    19321 tcctttacgg gtcaggcatc cgtttaagtg agtgtagcag tttgtcggta tcagacattg
    19381 attttgattc agaggtcatg ttgatccatg gtaaagggaa caaagagaga tatgctcctc
    19441 taggttcttt tgctcaagat gctctgcaag aatatttcag ggaaggccgc caggtattga
    19501 tgacaaaata tcacgaagag catgatatcg tttttgtgaa tcatcatgga aaaccaatca
    19561 cgcctactgg aattgaatat gtcttgaatc aaatgatcaa aaaaagcagt ttggatagtg
    19621 atatccatcc tcacatgctg cgccatacat ttgctactca tttattaaat aatggggcag
    19681 atttgcggac agtccaagaa ttgctaggac atgccaattt atcgactacc cagatctatg
    19741 cccatgtaac aaaagaaagt ttacaaaaaa actatcgttc tttccatcca agagcgtaaa
    19801 aataaggaaa agaacaaatt taggaggaaa caaacatggt cgaatcacca tttcattcaa
    19861 ccactatctg tgccgtcgaa aaagacggga aatttgctat ggctggagat ggccaagtaa
    19921 caatgggtga atctgttgtc atgaaaggca ctgcaaagaa agtacgacgt atctacaatg
    19981 gagaagttgt tgtaggtttc gccggaagtg ttgcagatgc gtttacttta gaagaaaaat
    20041 ttgaaggcaa actaaacgaa tataacggga atctacaacg cgcagctgtc gaattagctc
    20101 aagaatggcg gacacagcaa tctatgcaaa aacttgaagc gatgctgatc gtaatgaatg
    20161 ataaagaaat gcttcttgtt tcaggtacag gagaagttat cacaccagat gatggtatct
    20221 tagctatcgg atcaggaggg aattttgcgt tggctgcagc ccgagcaatg aaaaactatg
    20281 ctcaaaacga catgagtgcg aaagaaattg cagaaaatgc gttaaatatt gccgcagata
    20341 tctgtatctt tactaatcat aatattatcg tagaagaact gtaagaggtg agtagatatg
    20401 tatgaattaa aacaaacacc aagacaaatc gtaagtgaat tagatgaata tatcgtagga
    20461 cagcacgcag caaaaaaatc tgttgccgtt gctttgcgta accgctatcg ccgtttgcag
    20521 ttagatgaaa aaatgcaaca agatattaca ccaaaaaata tgctgatgat cggacctaca
    20581 ggggtcggta aaacagaaat cgcacgacgt ttggcaaaaa tcgtcaatgc accatttgtc
    20641 aaagtcgaag caacaaaatt tactgaagtc ggttatgtcg gaagagatgt agaatcaatg
    20701 gttcgggatt tagtagaaaa tgcgatccaa atcgtagaaa aagagcagta cagccgtgta
    20761 tacacaagtg ctaaaaaaaa tgcagaaaaa cgtttggtaa aaatcttggt acctggtatc
    20821 aaaaaagaac agaaacaatc cactaatcaa tttgaacaaa tgatgaatct ttttaatgct
    20881 agtcagcaaa ataccgctga accgcaagaa gaagtgacag aagatattcg aatcaatcgc
    20941 cgtacagtct tggaacaact acaaaagggt ttactggata gtcgtgaagt aaccattgaa
    21001 gttgaagacc aaaagaaacc tgcaccaatg atgaacaatg gattggagca aatggggatc
    21061 gacctaaatg aaactctagg tgctttgaca ccgaagaaaa aagtggaacg gacggtaaca
    21121 gtcaaagaag ctttagagtt attgatcaag gaagaatcag caaaattagt caaggatgct
    21181 gatatccata gtgaggcaat caaattagcg gaagaaagtg gaattatttt tatcgatgaa
    21241 atcgataaga tcacttctaa atcacagcaa tctggagaag tatccagaga aggcgtccaa
    21301 agagacatat tgccaattgt cgaaggttca caagtaaaca caaaatacgg gatcatccaa
    21361 acagatcacg tcctattcat tgcttcagga gctttccatt tgtctaaacc aagtgatcta
    21421 attccagaac ttcaaggacg cttcccgata cgggtagaat tagatgattt gacagctgaa
    21481 gattttgtga agattctgac cgaaccaaat aatgctttag taaaacaata cgtcgcgttg
    21541 atcggtacag aaaatgtgtc agttacgttt acaaaagaag caattgaacg gatcgctcaa
    21601 atcgctttcg atgtcaaccg tgacacagat aatatcggcg cacgcagact gcataccatt
    21661 ttggaaaaac tactagaaga tctattattt gaagcaccag atatgcaaat gggagaaata
    21721 acgatcacag aagcatatgt ggatgacaaa cttggctcaa ttgcggcaaa tgaagattta
    21781 agtcgctata ttttataaaa aatcggagga cgacaatgac agacttgcta acgaaaaccc
    21841 gcaagataaa cacacttttg cagcaaaaga atacttttga catgcaagca gatttgcctt
    21901 acgataaaat ggctgtcaca ctaggtggta ttcttgacag caacacctat atcataggaa
    21961 gtgacggagt attactaggt tataatgaac gtcacgatgt gaacaatgaa cgaatcaaaa
    22021 caatgtttgt tgagaaacaa tttcctgaat cttatactga tacagtggca cagttgatga
    22081 aaactgaagc taatatccca attactagtg atattactgc atttccggta gagtcaagag
    22141 agaaatatcc atttggtctg acgactgtcg ttccgatctt tggagctggc gaacgtttgg
    22201 gtacgattat cttatctcgt atggaacaat cgtttgcaga tgacgatcta gtcttagcag
    22261 aatatggagc tacggttgtc ggtatgcaga tcctttatca gaagtctcga agcatcgaag
    22321 cagatgtacg gagtgcaacg gctgtccaaa tggcaatcaa cacattatct tatagcgaat
    22381 tgaaggctgt acaagctatc tttgaagcat tggatggaga tgaaggtcgt ctgactgctt
    22441 ctaatattgc cgatagtatc ggcatcactc gttctgtgat cgtcaatgct ttacgaaaat
    22501 tggaatcagc tggaatcatt gaatctcgtt cgttaggcat gaagggaact tatttaaaag
    22561 tattgaattc ccgttttaaa gacgagttag caaaacacag ttattagaag taaggggact
    22621 ggggcagata gaaatgtttg ttccagcctt cccattttaa aaagtaagtc gaagttggag
    22681 gaatggcaat gactgttatt attcaaaatg accagctgat cgccgaaatc gctgaacacg
    22741 gtgcggaatt gatcagtctg aaatcaaaag aacatggttt agagtatatc tggcaaggtg
    22801 atccagcgta ttggggcaga catgcaccgg ttttattccc ttttgttgga cgtttaaaag
    22861 atgatcagta tgtttatcaa gggaaaacat atcaaatggg gcaacacgga tttgctcggg
    22921 atatggattt tgaagtcatt gaacatggac aagaaatggc tagtttcatc ttaaggagta
    22981 cgccggagac attagaaaag tatccatttg attttgaatt agtgatcagc tatgaattag
    23041 gaggagacgg catcacggtt cattatcaag tggcaaatac cggtgaaaaa gaaatgttct
    23101 tttctatcgg tggacatcct gctttcaatg tacctcttga agaaggattg tcatttgaag
    23161 atttctatct ggcattttct ccattgaaat caagattacg cctaccattg aaaggcccat
    23221 tcatcgatat ggatcaaaag acgttaggtc agacaaatac gaatctggct ttgaatcgtg
    23281 aactttttga gcaagatgcc ttggtttttg aaacaagggg tctaaatgcg tacgccattc
    23341 gaagtgaaaa atcttcccat agcgtaactt taagctataa agatatgcct tatgtaggga
    23401 tctggtctcc ttatccaaaa gaagcgcctt tcgtttgtat cgaaccgtgg tgcggaatcg
    23461 ctgacacggt gaacagtagt ggaaacttga tggaaaaagt agggatcaat caactaggtg
    23521 cccatgaaat cttcaagaca aaatattcca tcacagtgaa gtaagaggtt gtgaccgaga
    23581 agttagcagg gaacaccgtt taccaaggtg tgtaaaaaag ctatcctgta agtaataagg
    23641 gagtgtgaca tcgattt
//
LOCUS       DACCCS010000041        22857 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.042, whole genome shotgun sequence.
ACCESSION   DACCCS010000041 DACCCS010000000
VERSION     DACCCS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.042"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(104..1336)
                     /locus_tag="IVK82_001749"
     CDS             complement(104..1336)
                     /locus_tag="IVK82_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP6392813.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="IVK82_001750"
     CDS             1591..2160
                     /locus_tag="IVK82_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6392814.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="IVK82_001751"
     CDS             complement(2338..2778)
                     /locus_tag="IVK82_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP6392815.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="IVK82_001752"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="IVK82_001752"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP6392816.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="IVK82_001753"
     CDS             3732..4655
                     /locus_tag="IVK82_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP6392817.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="IVK82_001754"
     CDS             4731..5870
                     /locus_tag="IVK82_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP6392818.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="IVK82_001755"
     CDS             5863..6663
                     /locus_tag="IVK82_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6392819.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="IVK82_001756"
     CDS             6663..7490
                     /locus_tag="IVK82_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6392820.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="IVK82_001757"
     CDS             7468..8202
                     /locus_tag="IVK82_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6392821.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="IVK82_001758"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="IVK82_001758"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP6392822.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="IVK82_001759"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="IVK82_001759"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP6392823.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="IVK82_001760"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="IVK82_001760"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP6392824.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="IVK82_001761"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="IVK82_001761"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP6392825.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="IVK82_001762"
     CDS             11787..13820
                     /locus_tag="IVK82_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392826.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15143
                     /locus_tag="IVK82_001763"
                     /pseudo
     CDS             13864..15143
                     /locus_tag="IVK82_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15353..16159
                     /locus_tag="IVK82_001764"
     CDS             15353..16159
                     /locus_tag="IVK82_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6392827.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16171..17391
                     /locus_tag="IVK82_001765"
     CDS             16171..17391
                     /locus_tag="IVK82_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6392828.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17381..18967
                     /locus_tag="IVK82_001766"
     CDS             17381..18967
                     /locus_tag="IVK82_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6392829.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19006..21144
                     /locus_tag="IVK82_001767"
     CDS             19006..21144
                     /locus_tag="IVK82_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6392830.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21512..22495)
                     /locus_tag="IVK82_001768"
     CDS             complement(21512..22495)
                     /locus_tag="IVK82_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP6392831.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7176 a         3899 c         4712 g         7070 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt tatcgtgtat
    14281 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    14341 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    14401 gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg
    14461 ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    14521 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    14581 ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    14641 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    14701 gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc
    14761 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    14821 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    14881 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    14941 tggaggatac attgacttca taaccatggg ttccgattat ttatatcctg ttggctatgg
    15001 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
    15061 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    15121 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    15181 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    15241 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    15301 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    15361 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
    15421 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    15481 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    15541 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    15601 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    15661 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    15721 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    15781 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    15841 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    15901 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    15961 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    16021 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    16081 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    16141 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    16201 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    16261 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    16321 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    16381 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    16441 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    16501 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    16561 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
    16621 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
    16681 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
    16741 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
    16801 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
    16861 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
    16921 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
    16981 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
    17041 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
    17101 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
    17161 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
    17221 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    17281 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    17341 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    17401 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    17461 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
    17521 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
    17581 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
    17641 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
    17701 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
    17761 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
    17821 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    17881 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
    17941 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
    18001 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
    18061 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
    18121 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
    18181 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
    18241 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
    18301 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
    18361 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
    18421 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
    18481 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
    18541 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
    18601 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
    18661 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
    18721 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
    18781 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
    18841 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
    18901 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
    18961 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    19021 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    19081 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    19141 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    19201 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc
    19261 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
    19321 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    19381 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
    19441 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
    19501 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
    19561 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
    19621 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    19681 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
    19741 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
    19801 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
    19861 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
    19921 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
    19981 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
    20041 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
    20101 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
    20161 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
    20221 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
    20281 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
    20341 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
    20401 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
    20461 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
    20521 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
    20581 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
    20641 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    20701 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    20761 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    20821 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    20881 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    20941 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    21001 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    21061 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    21121 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    21181 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    21241 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
    21301 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
    21361 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
    21421 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
    21481 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
    21541 cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa
    21601 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
    21661 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
    21721 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
    21781 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
    21841 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
    21901 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
    21961 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
    22021 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
    22081 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
    22141 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
    22201 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
    22261 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
    22321 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
    22381 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
    22441 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
    22501 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
    22561 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
    22621 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
    22681 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
    22741 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
    22801 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtg
//
LOCUS       DACCCS010000042        22780 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.043, whole genome shotgun sequence.
ACCESSION   DACCCS010000042 DACCCS010000000
VERSION     DACCCS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.043"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(64..1290)
                     /locus_tag="IVK82_001769"
     CDS             complement(64..1290)
                     /locus_tag="IVK82_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6392832.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(1362..1520)
                     /locus_tag="IVK82_001770"
     CDS             complement(1362..1520)
                     /locus_tag="IVK82_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6392833.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            2208..2948
                     /locus_tag="IVK82_001771"
     CDS             2208..2948
                     /locus_tag="IVK82_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP6392834.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
     gene            complement(3040..4227)
                     /locus_tag="IVK82_001772"
     CDS             complement(3040..4227)
                     /locus_tag="IVK82_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6392835.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            4549..5391
                     /locus_tag="IVK82_001773"
     CDS             4549..5391
                     /locus_tag="IVK82_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP6392836.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            5388..6338
                     /locus_tag="IVK82_001774"
     CDS             5388..6338
                     /locus_tag="IVK82_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP6392837.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            6353..6586
                     /locus_tag="IVK82_001775"
     CDS             6353..6586
                     /locus_tag="IVK82_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP6392838.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            6917..7501
                     /locus_tag="IVK82_001776"
     CDS             6917..7501
                     /locus_tag="IVK82_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6392839.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            7632..8300
                     /locus_tag="IVK82_001777"
     CDS             7632..8300
                     /locus_tag="IVK82_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP6392840.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            8302..9726
                     /locus_tag="IVK82_001778"
     CDS             8302..9726
                     /locus_tag="IVK82_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6392841.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            9744..10313
                     /locus_tag="IVK82_001779"
     CDS             9744..10313
                     /locus_tag="IVK82_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP6392842.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            10423..12177
                     /locus_tag="IVK82_001780"
     CDS             10423..12177
                     /locus_tag="IVK82_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6392843.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            12131..13903
                     /locus_tag="IVK82_001781"
     CDS             12131..13903
                     /locus_tag="IVK82_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6392844.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            14426..14695
                     /gene="rpsN"
                     /locus_tag="IVK82_001782"
     CDS             14426..14695
                     /gene="rpsN"
                     /locus_tag="IVK82_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP6392845.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            14710..14889
                     /gene="rpmF"
                     /locus_tag="IVK82_001783"
     CDS             14710..14889
                     /gene="rpmF"
                     /locus_tag="IVK82_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP6392846.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            14921..15298
                     /locus_tag="IVK82_001784"
     CDS             14921..15298
                     /locus_tag="IVK82_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392847.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            15319..15468
                     /gene="rpmG"
                     /locus_tag="IVK82_001785"
     CDS             15319..15468
                     /gene="rpmG"
                     /locus_tag="IVK82_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP6392848.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            15492..16475
                     /locus_tag="IVK82_001786"
     CDS             15492..16475
                     /locus_tag="IVK82_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP6392849.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            16505..16627
                     /locus_tag="IVK82_001787"
     CDS             16505..16627
                     /locus_tag="IVK82_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP6392850.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            16645..18168
                     /locus_tag="IVK82_001788"
     CDS             16645..18168
                     /locus_tag="IVK82_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP6392851.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            18192..18431
                     /locus_tag="IVK82_001789"
     CDS             18192..18431
                     /locus_tag="IVK82_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392852.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(18624..19085)
                     /locus_tag="IVK82_001790"
     CDS             complement(18624..19085)
                     /locus_tag="IVK82_001790"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6392853.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(19280..19498)
                     /locus_tag="IVK82_001791"
     CDS             complement(19280..19498)
                     /locus_tag="IVK82_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6392854.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            19616..21232
                     /locus_tag="IVK82_001792"
     CDS             19616..21232
                     /locus_tag="IVK82_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP6392855.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(21473..22780)
                     /locus_tag="IVK82_001793"
                     /pseudo
     CDS             complement(21473..22780)
                     /locus_tag="IVK82_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         7724 a         3357 c         4274 g         7425 t
ORIGIN      
        1 ggcacttttc aattgtttga aaagtgtctt tttcttgttt aagaacacat ttaagaacac
       61 atactaatca catttattca atcctctctg aaaggcttca aaaacagttt tatctactac
      121 atctggtgta tttacgtata ttctcgttgt agaaatatct gagtgtgtta aagcactaga
      181 tatttgattc atatttgctc cgccttgtct agctatagtt gcataggtat gacgaagctt
      241 atgtggagat aaatgagcca attcaggatg tcgtctttta attgaattga ttcgatagtt
      301 taggtaatca gcgtgaacag ggcaatttac ttctccggat ggtttagtat aggtaaagag
      361 atactgatct ggtgtttgat taatttttaa tctcaacaat tgctcagctt gcactgattt
      421 ccatttgctt agtaatgcca ttacaatttc gggtacttga attacggtat cctttcgacc
      481 ttttgtaaat ttcttttttt gatatttatc caaagcatgt ttaaggcgaa ccgtttgttt
      541 ctcaaagtcg atatatttcc attgcaaagc atacgtctca ctttttctat caccaagata
      601 tagggtaagc atgaaaagag tatagtcatg aagagtaagc aagtgattat tcaagtcagt
      661 gtttactgct tctatccaag caataagctc ttctgacgat aatgcttgat taccttcccg
      721 tatccttctt tcttctaaag caattttttt aggtgcagtg atacattgaa taattttggt
      781 agttcggtta aattcaatat aatttagtat ttctgcaata tcaaacatag aacggacata
      841 tcctttgata atttttatat ttgcgtattc tttcgatttt tttgttaatt catctgatat
      901 tatctcagta tttgaattta gatagttcat tgcatactca ccaaacattg gaaggatgtg
      961 caatcggaat atatctttag tattactaat tgttacgtaa cttggagctc tttttgatct
     1021 agtagtctga cctagttcat atctaggaag ccattttgat tcatagaatt ccttaaattt
     1081 tatttttcct tttaattcta gtgaatttgc attctcctct cttaaaactg tttcaatttt
     1141 ccttttcatt gcattttcgg ctttaatagc caattgccta gttggaaata ctttttgata
     1201 cttttctgat gtaattccag tgatttctct gaattttttt ggatacctca gattagtttt
     1261 atatttccca tttgaaagtt ttgtaggcaa aattcctcac tcccttaaaa agtaaggcag
     1321 tatatttcgc tattcttagt atatgcggcg agaagaaatt ttcaaattgt tttaaatgta
     1381 ttcataaaat ctttgatatc ctctatgtca aagcgaacta taccgtcaat tctgattact
     1441 ggaaaattat atatacgaat ccacttatct aaagtattat tatcaattcc tagaaactca
     1501 caggtttgtt ttttattcaa gtagactctc aattttataa aatcatctcc taaaaaagat
     1561 tagaattttt gaattgcttt gggtaagcac ataggaattt cacctctccc cagtttacgg
     1621 cgagccttac ggaagtatca ttatttgctg ctattgtcat ggcattttgt tttcccagaa
     1681 caaaaagaaa tctctaagta tttgtcgcta atcaggtcat ggcgtacttg agtttctagc
     1741 tcgaatagca gggatcgtta ttcaaaaatt gtatttaatt gtcaatgatc aaaaataggt
     1801 tttttcctat tcactattaa taacgataaa catgtataaa aagacacact acgaaaaaag
     1861 ttaaatggag tttactgatt ttcatatttg tatatggaac agacatagtc ttgaataatt
     1921 tttataaatg ttttgatgct tatcaactta ttcaacccgc tatccacttt aagtataatt
     1981 tttccatacg ccaataaagt agttttagga gcatgttctc atttgagaac gctccttttt
     2041 tatatatttt gaaagctact tactatacgc gtagtttcaa atagttttgt cattctaatc
     2101 ttcagcaaca gaaaaaaatc taaattataa aatttagggt tgactaaaat ataatttttg
     2161 tataccatat gatcgtgata atcattatca tttagaagga gaaagttatg ttagaagtga
     2221 aaaagcttaa tgtttgttat aacgattttt tagcattgaa cgagatttct ttaaaaatac
     2281 aagaaggtgc tttaacaggt attattggtc caaatggtgc tggaaaatct acattgctta
     2341 aagctatgtt aaatattatc cctcatcagg gagaggtctc cattaataat gaagatatca
     2401 ataaaaaact ggtgaagatt gcatatgtag agcaaaaagc tgatattgat tttacttttc
     2461 caataaaagt aaaagaatgt gtatctatgg gaacatatgc cggaatgaaa gtttttcaaa
     2521 gaattaaaaa tgcagaatgg caaagagttt caaaggcttt ggaaaaggtc gatatgggaa
     2581 aatattctaa tcatcaaata ggagaattat ctggaggaca atttcaacgt gtacttttag
     2641 caagatgtct tgctcaaaat gcggatttca tatttttaga tgaaccattt gtagggatag
     2701 acttagttag tgaacgaata attatggata cgttaaaaga attgaaacat cagggaaaaa
     2761 cagtgctaat tgtgcatcat gatttaagca aggtaaagaa gtattttgat aatattattc
     2821 ttttgaatcg aaatctaatt gctcacggaa gtgtagaatc tgtatttaat gaagagaatt
     2881 taaaaaaagc ctatggagat actatattta ttggagagac taactatata gagtcaagta
     2941 aaaattagcc aaatcaaaaa atattttata acagataatt ccgaaaaaag ggtacaacaa
     3001 ataagcttct taccattagt ttttttgaat gttcaatagc taatcagaaa caattatata
     3061 aggctcgttt ttagtcaaaa tagcatgaat cacgtaacaa agcttacgag aaaccgcacc
     3121 taaggcagtt agatgatgtt ttccttcgga aacttttttc tggtaaaacg cactaaaaac
     3181 aggatcacat tgagaagcta ctaaagccgc cgaaaacaag gcttttcgca aatatggaga
     3241 ccctcgttta ctcataacat tatgcgttgc ctcatattgc cctgattgtg tcacggaagc
     3301 gtctatccca gcgtaagcaa cgagtttttt cgggttagaa aatttttgaa tatctccgat
     3361 ctcaccaaga atagtcgcac cgtttatagg accaattcca ggaattgtga gaataacaga
     3421 atttaaagtg tccattatgt gcttgatttc tctttccgtt tcctttattt ggctttcaat
     3481 gaattttaat tgttcaatca ttgaacgtag ttggaaggtg aacgcttctt gagcaaattt
     3541 tactccaaag gagttagaag ctgcttgctt caattggtcc gctttttctg ccttgattcg
     3601 gttcctgctt aactcagcta agtattgggc caaagtttct gaggaaatgt tttctaagtc
     3661 gatcggggca ctaaacgcca gcaataactc tttagaagct tttccgaaaa cgccctgctt
     3721 ggtaaaaatc gtcgcgtatt ctggaaagac ttgatccaat acggctataa tttttctttt
     3781 gaagtcgctc gctgtaccta ctaaataagt tcggtaacga gaaagttgtt tcaaagaaaa
     3841 aacattttca tcggataaga ccgtttcgac aaatgagcca tagcgcatta agtcagcaat
     3901 caaaacagaa tcaataatat cgttttttcg tttccgtatt tctgtacctt tacgccatcc
     3961 gtctgtttga aggggattca caacgtgaat caaataatct gattcgtgaa ggtatgaaaa
     4021 gatggacagc caatagtgcc ccgtggcttc catccctata acaaagtcac tgggtgctcc
     4081 ggaatagtcg accattcgct caattaatga ctctgctcct tcagtggtgt taggaaatga
     4141 aaaacctttt agtaatacct ttccttcctc gttcatgata gaagcaacgt gagtgcgttt
     4201 accgatatca atgcctaaaa agtacatgta tatctgacct ttcaaaaaaa gaattttgga
     4261 ttgaaatcca ctcaactgaa aagcgtcact accttgtacg agatacggag taaagtaagg
     4321 actttcaaca tctaactcat acgcaatcca cttatcagag agaggagcca gtctctcgag
     4381 tgcgggttca atggcccaag gggggaacgg cttagcctct caatccaata aatatattat
     4441 ctcaaaagaa atgagttaat accaaattaa aataacgatg gttcataggg ttatccaatc
     4501 aaaaccggaa ggttaaaacc ctaactctaa tatacaaggg ggaataatat gcttcaaaat
     4561 tttattaatg gtctgtatga ctttcacttt ttacagaatg ctctaattac atctgtagtt
     4621 atagggattg tttctggaac agtaggttgt tttattattt taagaggaat gtctcttatg
     4681 ggtgatgcta tttctcatgc tgttttgcca ggagtagcaa tttcttatat tttaggtatt
     4741 aattttttta ttggtgcgat tgtatttggt ctattagctt ctatcattat tacttttata
     4801 aaaagcaata gtgtaataaa aggagataca gcaattggta ttactttcag ttctttttta
     4861 gcattaggag ttatccttat aggagttgca aatagctcaa cggatttatt tcatatctta
     4921 tttggaaata ttttagctgt acaagatatt gataagtgga ttacaattgg cgtttctgtt
     4981 ttagtgttgg ttacagttgt tctatttttt aaagaattat taatcacttc ttttgatcca
     5041 ttaatggcaa aagcaatggg gatgaatgtc agtttttatc attatctatt aatgattcta
     5101 ttaactttag tagcagtaac agctatgcaa agtgtgggaa ctatattaat tgttgcaatg
     5161 ttaattacac cagctgctac agcatattta tatgcaaaca gcttaaaaac tatgatattt
     5221 atttcggcga cagtcggagc gtgctcatca ttgctaggtt tgttcattgg ttatagtttt
     5281 aacatagcag caggatcaag tattgtttta acagcggcat tcttatttgt aattagtttt
     5341 ctaatctcac caaaacaaaa atttttaaga aagaaggaga gaaagttatg atgagaaaat
     5401 ggaaagcagt actgggaagt ctggggattt tgattgctct ttttatattc ggggcatgtt
     5461 caacaaatag taaagacaaa gatacagtgg cttcgaacga aaaattaaag gtagtagtta
     5521 ctaattcgat tttagcagat attactgaaa atatagcaaa agataaaatt gatttacaca
     5581 gtatcgtacc tattgggaaa gatccccacg aatatgaacc tttgcctgaa gatgttcaaa
     5641 aaacttcaaa agcagatttg attttttata acggtgttaa cttggaaact ggaggaaatg
     5701 cttggtttac aaaattagta aaaaatgcga acaaagagga aaacaaagac tattttgcag
     5761 caagtgatgg catagatgtt atttacttag agggtcagag tgagaaaggg aaggaagatc
     5821 cccatgcttg gttaaattta gaaaacggta ttatttacgc taaaaatatt gaaaaacagt
     5881 tagcggaaaa agatcctgat aataaaaaat tctataaaga aaatctagat aagtatattg
     5941 aaaaattgga ttctctagac aaagaagcta aatctaaatt tgcttcaatt ccgaatgata
     6001 aaaaaatgat tgttacaagt gaaggatgct ttaaatattt ctcgaaagcg tataatgtgc
     6061 cttctgctta catttgggaa atcaacactg aagaagaagg aacaccagat caaataaaac
     6121 acttagttga aaaattacgc acaacaaaag ttccctcctt attcgtagaa agcagtgtgg
     6181 acgatagacc aatgaaaaca gtatcaaaag ataccaatat tcctatctat tcaacgattt
     6241 ttactgattc aattgcagaa aaaggacaag atggtgatag ttactatgcg atgatgaaat
     6301 ggaacctgga taaaattgct gaaggccttt cgaaataatt tggagggtag gtatggtaca
     6361 aattaaaacg actactatac taatttattt actagcaatt caggaattat caaaaagaaa
     6421 aaagaaatta aaaaataatg atttagcaaa agttctaagt gttagtccag catctgtaag
     6481 tgagatgctg gctaaactta gatatgagga ctatttagat ataggatttc aattaactga
     6541 taaagggaaa aaatttattg acgattacaa aaaaggtttt aagtaaaaga ctttattttt
     6601 agggaggata agaaaatggc tattggagtg attatgatag gacttaccct aatagctgga
     6661 tataattttt ttaaatcttt ctcggattat tttagaaaag gtgaatgatt tagatgagtc
     6721 tattaattct tgtaggattg ctttttgtat cagttatttt gctctttctc attagtttag
     6781 ctctaaggaa tgagttcagt aatagacgtt aacgattgaa acttcaagtc ctgtgtaaga
     6841 taatatagtt aattcatctt attaggagga agtacccata gaaaccagag attatattac
     6901 aactcaccaa aaattaatag aaagtggact aagccatttt gaaaaagaag gatttcagca
     6961 aactaagata aggcaactgt gcttgaccgc aggcgtaact acgggtgctt tttataaaca
     7021 ttttacttct aaggaagcgt tatttgaaga aattattcag ccgcatatta atgcgcttat
     7081 taatatgtat aatgaagagt ttaccaaata cctcttaaac ttagaaaata tagatataga
     7141 gaaaagttgg agagagaact tcaatgaatt agatccattt attaattata tttttgagaa
     7201 taaacgtata ttcgatttgg ttttattcca atcagctgga accaaatatc aagatattgt
     7261 tgaaaaaatt acatcatttg tgacaaaaaa gacagtctgt tgtctagata agttaaagac
     7321 tatcggaatg atcaatcaaa atttagtctt taatgaagaa gaaatccact tctacattta
     7381 ctcattctac gctacattct atgatatatt gaagcatagc tattcaaaag aaaagactat
     7441 gctattaacc agaagattgt atcaattttc tgttcctggg tggattgaat tactgagtta
     7501 attaaaaaaa agcttgcatt gagaattgtt ctcagttatg ctttttgata agatcgcact
     7561 gttatctgaa taacggtgca tttttgtcag cttaagataa cagtgcgatc ttattttttg
     7621 gaggtgaaag agtgtcaaaa atcaatccgt taaccttgat tactttaaat attttttttc
     7681 cggtcattat ttttttagga aagggaaata tatttcaagt ggggtgtttc attattgcta
     7741 cagttttagt attgatgttt cgtaaaataa agacatgtat ttatttcatc ttgatttatt
     7801 cagttctgtt tttagtaatg aaaattattg atgctatcaa gatagagttt tttactatat
     7861 tttttgggac atccttatat attgtatttc gaatgttgcc ggtcgtaatg atctcatggt
     7921 tgttggttag tgtctatact agcaatgaat tattatcctc tttagaaaaa atacatttac
     7981 cgaaaaaaat aatgttaagt attacagtag tgatcagatt ctttccgact tatcgttctg
     8041 aaataagaat gattaaagaa tctttaaaaa tgagaaacat tcgcttgtct ttttttcagc
     8101 ctctgcaata tttggaattt ctgttagttc ccgttcttat acgtgcaact atgattgctg
     8161 aagaaatgac cgctaatgca ataacaaagg ggatagaatc tcctgtgaaa agaagttctt
     8221 actataaaac aaacatgagc ttattagact atatcatcct gttttccgtt actatttttt
     8281 tcttaagtat tttgatttga ggtgaaagaa ttgtttgaat ttaacaatgt ttctttttca
     8341 tataaggatg ttaaggaaca agcgcttgtt gaccttaatt tttcaataaa aaaaggcagt
     8401 cttacattga ttactggttc ttctgggagt gggaaatcca cactattaaa gttaatgaat
     8461 ggacttattc caagacttta tgacggaaaa ttggttgggg aaattttatt tgaaaataac
     8521 tcgctacaat ccttaacaca agaaagtata tcaaaaaata ttggctacgt aagtcaaaat
     8581 cctagaggac aattctttac aacaaataca acgagtgagt tagtattttc gatggaaaac
     8641 tttggattta caaaagagca tatggataaa agaatcaatg aattagctga attattagat
     8701 ctttcaaata tattagataa aaacattttt tcgctatcaa gtggtgaacg gcagaaaatt
     8761 tctattggat gtagtttatc gctaaactct aaattaatta tattggatga accgtcttca
     8821 aatttggact tcaaagcgac taagaaactt gctgacttac tttgtgattt aaaaaataaa
     8881 ggatttacta ttgttatagc tgaacatcgt ttttactatt tgaaagaatt aattgatcaa
     8941 gttctatttg ttaaaaatag aactgtaaaa actacaacta ttcagttgtt aaaaactgaa
     9001 aacaataacg cattaaggaa atttgacatt tttaatagtt cgattgaagt tccctatatc
     9061 gaaaaggtaa gtgcagatgt attaaaagtt gaagaaatta cttatgaaaa tattctaaaa
     9121 gacataaatt ttgatgtcaa agttggagat gttattggtt tggtagggaa aaacggtgtg
     9181 gggaaaacaa ccttactaag aatactcatg aagattattc gccctaccaa aggaaagata
     9241 atcgaaaata aaaaaaattt gtcatcgccg tttttagtaa tgcaagaaat ggattaccaa
     9301 tttttcacag aatcagtgag gagcgagtta gccctgggga atgaaatagt tagtaaagat
     9361 cagcaagaaa aaattttaaa aaaaatggaa ctttataaaa tgaaagatca attaccgttt
     9421 gatttatccg gtggagaaaa acaaagatta ttggtttcta tagcttcatt gtcaaaaact
     9481 aatttatttc tatttgatga acctacaagt gggttggatt atatcaatat ggaaaggatt
     9541 gctgacctta ttaaagactt aaaagaaaag ggtgcagtag ttatagccac tcatgatccg
     9601 gaatttcttt ataaaacatg caatagaatt atttatttaa aaaatggtgg aataaaaaaa
     9661 gatataaaac ttagagtaga agattcaact ttgatagaac agatttttaa tgacataacg
     9721 aaatagaatt gaggagaata tatatgaaaa caaaagatta tatttttatt ggtttagcta
     9781 cagttatttc cctagtaatt tacttttttt caataatggt tagtagtatt ggtggggcgt
     9841 ttggtcacag catatcccca gggatttttg gattattatc tggaatcata tttgtttata
     9901 ttagctataa ctaccctaga aaaggaattt ttactattta cacaattgtt ttattgttat
     9961 tttttacgtt aatgggcggg gcgtacttac cttggtttat cagttcgatt agtatggcga
    10021 tcttagctga tattattcta tctgtatttg gatatgatcg agcaattcct caagttgtgt
    10081 cttgggttct aatgcaacta ggcagtgctg ccggacagtg gattcccata tggttcttta
    10141 cagatcgttt tcgtcaagac tggatagata gaggacaatc tgctgcaaca atggatgcaa
    10201 tgatccacta cgcggtgggt atatggggaa ttatttcagt gttagttgta gcaagtttat
    10261 ctatgattgg tgtattgatt ggaagaaaag tcttaaaaaa atacaaaaaa tagaacgtag
    10321 tttaccttct atctgtttgt acatctctaa tttacgctat tacggaaagc attaacagtt
    10381 gtaaacgaat aagagaagaa attgaatgcg ggggaggaaa aaatgaaagt atacaaaaaa
    10441 ttatttagtt atgtccctaa aatgaaatac tacggatggt tagccacttt attttctgat
    10501 ttttctgtcg tattaacagt ctatggatat tacagtattt acaaatttct tcagtctcta
    10561 attattgtag gagatgaata tttagctaag agttatgcgg ttcaaactgt tgcttggtta
    10621 actttaggag ctttatttta tattttttct ggcttatttt ctcatatcct tgggtttcgc
    10681 ttagagacta atttgagaaa aaagggaatt agcggtttaa cagaagcaag ttttcgtttt
    10741 tttgatttaa attcttcggg atatattcga aagacaattg atgacaacgc ggctaaaaca
    10801 catatggcaa ttgctcactt gattccagat agtgctcaag ccatagtaac acctgtttta
    10861 tcagttgcct taggcttttt cattaacttt aaaattggat tggttctaat cgccatgtta
    10921 gtaattggtg tattcttgtt aaagaaaatg acagagaata caaattttat tcaaaaatat
    10981 caagaatcgt tagatgtgtt aagctctgaa acggtagaat atatccgcgg aattcaagtt
    11041 ataaagatat ttggtgctaa agtaacatca tttaaagcct tgaataaagc aattaacgat
    11101 tatgctaagt ttgcatataa gtattctttg agtgggaaaa caccttttgt tctttttcaa
    11161 tggctatttt ttggcatagt ttgcattttg atagttccta taaccttttt tataacttct
    11221 cttaccactc ccaagatctt agctgttgac ttgttgatgt tgtttttttt aagtggaatt
    11281 atttttgttt ctttcatgag aataatgtat gtaagtatgc acttgtttga agcaagatat
    11341 gcagtcgaaa cactagactc cctttattta gaaatgaagc aagaccaact aaaatatggg
    11401 aacgagacac agattaaaaa ttgtgacatt gcatttgaaa atgtcagctt ttcatacaaa
    11461 gaagagtgtg ttttagaaaa tttcaactta gttttagaac aaaaaagaat ttatgcatta
    11521 gtaggaaaat caggaagcgg aaagagtacg atagctaaac taatttcagg tttctacaat
    11581 gtggataagg gacagattaa aattggtggt aagcctttaa atcactattc aaaagacaca
    11641 attattaaca caattgcttt tgtctttcaa gatacgaaac tattcaatca gagtatttac
    11701 gataacgttt ctatagcaaa tcctcaagcg ccagcaagta aagtatttga cgcgctaaga
    11761 ttagccggtt gcgaatcgat tttagaaaaa tttcccgaaa gagagcatac cttaattggt
    11821 agtaaaggag tttatctgtc aggaggagaa aaacaacgta tagctattgc cagagcaatt
    11881 ttaaaagatg caaagattgt tattatggat gaagccagtg catctattga tccagataat
    11941 gagtatgaac tacaaaaggc ttttcaatat ttgatgaaag aaaaaacagt tattatgata
    12001 gctcatcgat tatccagcat taaatctgta gatgaaattc ttgtcttgtc agaaggaaaa
    12061 atagtagaac gtggaaatca taatgaatta atggctaaac atcgagtgta taaacaattg
    12121 gtcactaatt atgagaatgc aaatgattgg aggcttgaca atgaaaaatt tttataaaaa
    12181 aaaatttgct ttaactgatc agggcgcaga agctttaact aaagcttcta tttctagttt
    12241 ttttgtatac tgcattaata tggtaccagc attcattata atgatgctga tagacgaatt
    12301 agttttagaa aatgcaaagc cccgttggct ttattttgca gtatcgtttg ttactttgct
    12361 tttcatgtat tggttgttag atagggagta tgaaaactta tataatagta cttataaaga
    12421 aagtgcgcat ttaagagtgc aaattgcaga cgatttgtca aatttaccat tatcctattt
    12481 ttcaaaacat aatttatcag atttatctca aactatcatg tctgacgttg aaggtattga
    12541 gcatgcgatg agtcatgcaa tacctaaagc cggcggtatg gctctgtttt tcccttttat
    12601 ttcagtgatg cttttggttg gtaatgtcaa aatgggatta gctgttattt tgccaacgtt
    12661 atttagtttt gtcttaatct tgttatcaaa gaaatcccaa acgaaagcca atactaaata
    12721 ttacgatact ttgagagaaa actcggaaga atttcaagaa actattgaat tgcagcaaga
    12781 gattaatagc tttaatctat ctaaaaaagt tcaagacaga cttttcaaaa aaatggaaga
    12841 gagtgaaagg attcatttaa aggtagaatt aagtactttt tcagtcatgg ccttatcctc
    12901 tattttctca tatgttagtt tagcagtagt cattctagta ggtgttcact tactgttaac
    12961 gggtgaagtg actatactct acgtcgttgg ttacttacta gccgcaataa aaataaagga
    13021 ttccttcgat tctatgaaag aagcagtgct tgaaatattt tatttagctc ctaaaataca
    13081 gcgaattaga gctatgaagg aaaccagtat ccaagaggga tcagatagtc ctctgaaatc
    13141 ttttgacatt gagttgcgag atgtctcttt ctcatatgat aacaataccc caattttaga
    13201 tcatatctct tttacggcta aacaaggaga agtgactgcg ttagtaggtg ctagtggttc
    13261 cgggaaaact agtatcttga aattagtgtc tagactgtat gattatgatg aaggctgtat
    13321 tttaattgat gggtatgata ttaaacgtgt atctccagct tctttgttta gcaaaatcgc
    13381 tattgttttt caagaggtaa ctttgtttaa tacaagtatt ttggaaaata tacgtattgg
    13441 aaatagtcaa gcgtcagatg aggaagtgaa aaaagcagct cgtttagcta actgcgaaga
    13501 ctttattgaa aaactacctg acggttatca tacattggtc ggagaaaacg gaagttcact
    13561 ttctggagga gagcgacaac gcctatccat agcacgggct tttttgaaaa atgcgccaat
    13621 tttaatttta gatgaaatta ctgccagttt agatgcggaa aatgaaaaac gtattcagga
    13681 aagtttaaat cgtttaatac aagataagac agtactaatt atatcgcatc gcttgaagtc
    13741 tattgaaaag gtaaataaaa ttgtagtgat ggatcaagga aaactagttg atcaaggtac
    13801 tcataatgag ttgtatcgaa ggtcagaaat atacaaaaat ctcataaaga aaactaaact
    13861 atctgagaaa ttcgtctatg aaaaagaagc tcaatcacga tgatgaaata ggctttaaat
    13921 taacctacaa tttttgggat atagttataa ttctttttca aaaagaataa ttttattaaa
    13981 cagcatctaa ttttgaatac acgaaagaat cagtatgatt gaattagttc acttaatgtc
    14041 tattcttata atataaggaa taatcaaagt tttaaaaaat tggcgataaa ccagttaaac
    14101 ataaaagatt gaataaaaag ttatagtttc tttcatattg ccgttttaag tagaggaaaa
    14161 gctacttaaa acggtatttg tgtttgattg gactaataaa agtgaattct tatttttcag
    14221 agcctaaact gcatattctt ttaaatgatt tgattatctc tatgtaccga taaattcgtt
    14281 ttcaaatagt tatctattca tatgagtttt atttcatgtt atttattgtt gacaagagca
    14341 actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt acgatttgag gtgtctaaat
    14401 caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg aaaattgcta aagcaaagaa
    14461 acaaatggcg atgattgaaa agtatgcaga taaacgtcag gaattaaaag ccgcaggaga
    14521 tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg aatcgtctga ggcttcgtga
    14581 tcagacggat ggtcgtccaa gagggtatat gcgaaagttc ggtatgtccc gtattaagtt
    14641 tagagaactt gcccatcaag ggttgattcc tggagtaaaa aaagcaagtt ggtaaaagga
    14701 ggaaatatca tggcagtacc agcaagaaaa acatctaagg cgaagaaacg gttaagaaga
    14761 actcatcaaa ctgtagtaaa accagaaatt tcatttgacg aagcgaatgg tgattataga
    14821 cgtagccatc atgtatcttt aaaagggtac tataatggaa agaaagtaat taaaggagct
    14881 tcaaaatagt ttaatatata acttttagga gggaatcaaa atggaaatta tttacccacc
    14941 tttagttgaa gaaggattaa agtattatct tgaaacgacc cagcaatcgc tagataaatg
    15001 cactttttat cgttcgatgg tagagagagg aattattact gaaacgggtt taccgaccca
    15061 acaggcaatt gaaaatggat tagtcaaaga ttactatgag gaccaaggct tatcatttga
    15121 tgatttttta agaatctatc caatttttga agagtatgac gaggagttat ttcaatgtat
    15181 cgatggttat tgggagatac ctgttgatat gaaagaaaat ttagtttcac aattggaatc
    15241 tggcgagtta acttttgaag atgcacaaca aattcaagca tatcttgaag atcgataagt
    15301 attgaaggag gaagatttat gcgtcaaaat attattttgg aatgtgtcga aactggagaa
    15361 cgtctgtatc tcaccagcaa aaataaacgg aataaccctg aacggttaga attaaaaaag
    15421 tattcgccaa aattgcgcag acgggcaatt tttaaagaag tgaaatagct aagtggtaaa
    15481 aaggtgacaa aatgggcata ccagttacaa tcgtttctgg gtttttaggg gctggaaaaa
    15541 caacgctaat caatcaagcc ttgcaagtct caccctaccc cagggaagaa attattataa
    15601 ttgaaaatga gtttggtgaa gttgggattg atcacaaatt attagttcat agcgaggaac
    15661 gggtgttaca actaaataat ggatgtatgt gttgcagtct tcgcagtgat ttattagcaa
    15721 tattgatttc tttactggaa acaatagaag aacatcaaca accaatttca caaatcatca
    15781 ttgaaactac aggtatagca gatccccaac caatcattca aacactttta acagcgcctc
    15841 aactaagagg aagagtatat atcgatagtt tattgactgt tattgatagt gacagttttg
    15901 aacttattga aagagaacca caagccctta agcaacttgt catggcagat cgcatttttg
    15961 tgtctagaaa acaaaacttg aatttatctg cactaaaaaa attacagaga gaaatcaaaa
    16021 gtattaaccc tttatccgat attcgaatgt tcacacatag tgaaacgatt caaaaaaagg
    16081 atttttataa tttgaaaaaa tttaatcatc ccttagtttt tgaaagtgat tcatcagaaa
    16141 atcactataa tggcaaatac tatcatcaac accatcaagt acatgagttt aaatcaattc
    16201 tcttaacttc agatcaagac ataaaaaaag attttttact ccaatggatt gactggctga
    16261 ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt gattgcttta caagagcagg
    16321 attttatagt tgccatgcaa gaggtaaacg aacaggttaa ttttcaatac accactcgac
    16381 aaaaagacca aaccactatt attctaatcg gaaaaatgtt ggatgaagaa aggatcaaac
    16441 agagttttga taaattactg gcggaaagca aataatgata agtagtgatt gggggtaatt
    16501 tctaatggag aaaacggata tatctagtgc atatcgacga cttaaaagcc cgaatattaa
    16561 aactagaaaa agggcattaa aaattatcaa agaacataaa cgaaataaaa tgaagaaatt
    16621 agcataatta aggaaggaaa aagaatgaag aaaatcagtg taggacttat tgggatagca
    16681 gctctaggat tattgggagc ttgctccagc actaatgatg caaaagtatc gaatgataaa
    16741 gatggaaaat tagaaattgt aacaacattc tatccaatgt atgatttcac caaaaacatc
    16801 gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg gatcagaacc tcatgactat
    16861 gagccctcag ctaaggatat ggcaaaagct catgatgctg atgtttttgt atatcataac
    16921 gaaaacatgg aatcatgggt accaaaagct aaagaaagtt ggaagaaagc tgggcctaat
    16981 gtagtagaag gaacaaaaga catgatttta ttgccaggta gtgaagaaga agatcatgat
    17041 cacggagaag aagatcacca tcatgaacta gatcctcata cgtgggtttc acccaaaatg
    17101 gcaattaagg aagtttcaaa tatcaaggat cagttagtta aattgtatcc gaaaaaagca
    17161 aaagtatttg agacaaatgc tgaaaagtat ttgactaaac tgaaacggtt ggatgctgac
    17221 tataccacta gtcttaaaga agcgaaacaa aaaagttttg tgacgcaaca cgcagccttt
    17281 ggatatttag ctttggatta tggattgaaa caagttccta ttgctggact aagtcctgaa
    17341 gaagagccat cttctggtcg attagctgaa ttaaaagagt atgttaaaaa aaataagatc
    17401 aattatattt actttgaaaa gaatgctaat gataaaattg caaaaacttt ggccaacgaa
    17461 gctgggataa aattggaagt actgaatcct cttgaaagtc ttacaaaaga acaaatggat
    17521 aatggtgaag attatgtttc agtaatggaa gacaatctaa aagcgttaga aaaaacaact
    17581 atggttgctg gtgaagaggt agttccagaa aaagaagcga aggatgaaaa gacagttgct
    17641 aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat tgagtgatta tacaggcgaa
    17701 tggcaatctg tttatccgtt gttaaaagat ggaattttgg atgaagtttt cgattacaaa
    17761 gcaaaaatca ataaagacat gacagctgcc gagtacaaag attattatac tactggctat
    17821 aagacagata ttgatactat caacattaaa gacaacacca ttgattttgt agtcaacggt
    17881 gaacatcatc aatacacata caaatatgtt ggatacaaaa ttttgaatta tgaaaaagga
    17941 aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag gtgctggtcg ttttaaatat
    18001 attcaattta gtgatcatgg aattgcacca agtaaggcag agcatttcca tattttcttt
    18061 ggtggagaaa gtcaagaaaa actctataat gaattgcata attggcctac tttctatcca
    18121 gcttcattgt ctaaacatga aattgctcaa gaaatgatgg ctcattaaaa ttcaattttt
    18181 ggaggaaata catggtaaga aaagtattat tgtctatgga ttccaagaat attgatgctt
    18241 atcggcgtta tcgatttgca aagcaaatga gattaaatgg gagtacatta gctgatattg
    18301 ctgcaacttt acatgttagt attgacacag ttcatagagt attaaaacgg gatcctgatt
    18361 gttgttttat caaggtcaat actggaagaa gtagacgttt agcaaaacgg cataatagct
    18421 tggccaatta attgataata ccagagtaga tcatttttcc cttatttgga tttgatctgc
    18481 tttttattat gtcttgaatt gtcaaatcaa gcgcaacact ttatcaaaga agacttcata
    18541 aggtgttctc caatctaaac actttctagg tctgttattc agtttttcaa tcatctgatt
    18601 tatcgttgag ttatctaccg tagctatctc tttcgatttt ggcaagtatt ctcgaatgag
    18661 tctattcgta ttttaatttg ttcattgttg ccaagggcat gaggatctgg aaaataaaag
    18721 ggcatattat tcaatgcttc tgtaactgcg aaatgattgg agaattcttt tcctctatct
    18781 ggagtgatcg ttttgaccgc ctcatttgga atactgctta aaagttcaat cattttctcg
    18841 ctcacataag cggctttctt tctggggacc ttctttcctg ctaaaaggaa gcatgatttt
    18901 ctgtctgtca aagtcactaa acaagtacca ccagttttgc cagctacaat atcggctttc
    18961 caatgaccaa aatcacttct atcatctgcg gattttggac ggccatggat cgaatgagta
    19021 aggcaaatct ttccacgaga ctcgacatga ttttttgtat gtcttgtttt tcctcgatgg
    19081 tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg gttctgtatc gaaaagatca
    19141 ttgcaaattg cacgttagat cgtgataaaa ctaattgaaa agtccatatg ttcatgcttc
    19201 aaacgattcg agatttcctc tggcgaccac tgaaaatcga caaataaatg acgaacaatt
    19261 gaccaaactc acggatcatt tagaatgtga taacgtccgc atttttttct tcgataagca
    19321 taattttttt ccgctttgga aggtgaatag tttttttctt gtttcttctc agttcatgta
    19381 aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat actaatagaa aatccttctt
    19441 cgtgaagcag aaagattctt tctcgttcga gtatagtaag atgacgatac tggctcataa
    19501 ttattttcct tctaggagtg ttctcgacaa acttatacta gttggatatc gattatgact
    19561 catttttatg tgttgcactt aaagtgtaaa ttcaaggtat agaaagaggt tccctatgaa
    19621 aaaaatacct attacaatct taactggcta tcttggttca ggaaaaacga tacttctaaa
    19681 tcatattctg tacggtgatc acggaaagga aattgctgtc attgtaaatg acatagggga
    19741 agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt aaaagaactg gagaaaaact
    19801 tgtttcgatg ccgaatggat gcatttgttg tacattacgc gaagatttga ttgttgaact
    19861 agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt attgaagctt caggaatcag
    19921 caaaccaatt ccaattattc aagcaattat attagctaaa aaccctttag acattgattt
    19981 gacccaacga ttagagattg gtgccattat tatagtagtg gatgtaaaac gtttttggga
    20041 taatttcgaa acgggtgaaa agattcagga aagaaaagct gaaggtaatg tagttgaaga
    20101 agaacaggat attcgtggct tgttaattga tcaattcgag tgttgtaaca tattactttt
    20161 aaataatagt gacttagtgt caaccaagac gatttacaaa attaacggtc taattgctaa
    20221 gttacagtcc aatgccaaaa taattaggac aattaaaggt gaagtagact tatcgaaaat
    20281 tctaaataca aatcttttta atatagatga gacttcctat tcagtgggct ggttcaaaga
    20341 gttagagctt ggctatcaaa atcatattcc tgaaacagaa gaatatggta tctcaagttt
    20401 tatctatcga aatcggttac tccttgacgc acaaaaattt gcccatctta cggataaaaa
    20461 ttttcctggc aatattacaa gagcaaaagg gtttctttgg tttgcccaac atagtgattt
    20521 ttcgacgatc tttgaaacat cgggtagcta taaaagtatt gaatcattaa attattgggt
    20581 agctagttta cctaaaattg agtgtcaaat gattatacaa aaaaatcccg aattgaagga
    20641 agcctggcat ccgttgtttg gtgaacgcat taataaacta gtttttattg gtatgaagat
    20701 ggatcaacaa tgtattaagg aacaacttga gggttgttta actgattctc ttaatgcaga
    20761 taatttccga aaaatttcac catttccatg gaaaaagtct aatatgttta gccagatatc
    20821 gttaatgaaa tttttaagaa aagctggtgt caagataggc gatgtcataa ttattggcat
    20881 tttgattatt acctctttta ttcccttatt tgtattctct atagaaaagg agaatgaaac
    20941 aactgaagat gctatcaaat atgccatcgt tcgaattgat ggcaaggaaa tggatcgctt
    21001 cgatttggat aagatcaatc ataaactcgt gaattatcat cctgcgaagg gacaatataa
    21061 tattgttgag attaaagatg gaagaattcg agttaaagaa gataatagtc ctgatcaaat
    21121 cgcggttaga actggatgga tctcaaaacc cggtcaaacg agtatttgtt taccccataa
    21181 gttagttatt agtattgaga aaaaagaaag cacagattac tacatttact aatctggaaa
    21241 tagatgagga ggtttttaat aagaaaaaga tattattatt aataatagca ggggctttat
    21301 taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt gacaacaggc acaagtatct
    21361 ttaacgggac ctgcaatggt ttagttaaca gtcggttaga ttacttattt tggctcttcg
    21421 tcaatgagtg ttggtgaaga aattcaatag aataatcgag atactcacac ggctagacag
    21481 ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag ttgggtagta ttcttagttt
    21541 tcttaaaata ttttcctcga tataaaaaga ttcgttctcg gtagttttga aagtttctat
    21601 agccataggc aattcgtttt agtactttga tatggttatt caaacattct atagggccgt
    21661 tggaataggg aacgcgcaac gctcgtttga tttctgtttg gtatttcttg aaggtcgtga
    21721 acactggttg gtagtgctct ggcaactcct tgtagtcttc ttttaggagc gcgacgaatt
    21781 caggaacatc ttgtcttctg ataacggaaa gaaaatcctg gtagacttcg tagcctcgct
    21841 ttaaggaatc atcataagat aatagccgat cgactatctc tgcttctgtc agatgatctc
    21901 gaaatgacgg acgccagtag cgatgctgac ctttcaattc ccacgcattc ttttgaagta
    21961 gtttccagta tttctttaag tgcttcgacc gacgatcccc ttttttaaag gtcttcatta
    22021 cttgaattcg gtggttggta aaagcccgac caatgtgttg gctaatatga aagcgatcgg
    22081 cgacgattcg ggcattcgga aaacactctt tcaccaatgt gcgatagggt gtatagaaat
    22141 ctgaaaccac tagacgcact tgttcacgga cttttatagg gtagcgcaag aaatagtcgc
    22201 gtaaaggatt gagttgccga ttttccacaa tatcaatcag ttgatgggtc ttgccgtcca
    22261 tcatgacaaa gctcatggct cccgagacat ttttcactga tttgaattca tcgaaacaca
    22321 ccacttctgg taacagctgg ctcaaagaaa tggttttagg ccgaaggctg cgaagtattc
    22381 gaatgacagt ggtcggagaa atgtgcttca tacgtgcgat caaagacata gaaagaggtt
    22441 cgcttaatag ctcaagaatg gcttgtttga ctctgcgagc aatggagcag cgacgatcac
    22501 taactgaatc ttctgctaaa aaagtctttc cacagtgttt acatttgaag cgctgtttgt
    22561 taatccgcag aatggtcttg tagttactga catcattcaa taagatggat acttttttcc
    22621 agccctattt aatgatcgtt tgttcatttt gatagtggcg atgaaagcat tcctccggtt
    22681 gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc attgattcgc tctttgcgaa
    22741 aggcatcttc atgaaagata agatttaggt ctagaatatc
//
LOCUS       DACCCS010000043        22001 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.044, whole genome shotgun sequence.
ACCESSION   DACCCS010000043 DACCCS010000000
VERSION     DACCCS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.044"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            231..1235
                     /locus_tag="IVK82_001794"
     CDS             231..1235
                     /locus_tag="IVK82_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6392856.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1236..2180
                     /locus_tag="IVK82_001795"
     CDS             1236..2180
                     /locus_tag="IVK82_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6392857.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2180..3142
                     /locus_tag="IVK82_001796"
     CDS             2180..3142
                     /locus_tag="IVK82_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6392858.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3169..4083
                     /locus_tag="IVK82_001797"
     CDS             3169..4083
                     /locus_tag="IVK82_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6392859.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            4109..5890
                     /locus_tag="IVK82_001798"
     CDS             4109..5890
                     /locus_tag="IVK82_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6392860.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6238..6924
                     /locus_tag="IVK82_001799"
     CDS             6238..6924
                     /locus_tag="IVK82_001799"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP6392861.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6944..10525
                     /gene="smc"
                     /locus_tag="IVK82_001800"
     CDS             6944..10525
                     /gene="smc"
                     /locus_tag="IVK82_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP6392862.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10534..11343
                     /locus_tag="IVK82_001801"
     CDS             10534..11343
                     /locus_tag="IVK82_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6392863.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11354..12352
                     /gene="ftsY"
                     /locus_tag="IVK82_001802"
     CDS             11354..12352
                     /gene="ftsY"
                     /locus_tag="IVK82_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP6392864.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12539..14806
                     /gene="gshAB"
                     /locus_tag="IVK82_001803"
     CDS             12539..14806
                     /gene="gshAB"
                     /locus_tag="IVK82_001803"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP6392865.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14895..15347)
                     /locus_tag="IVK82_001804"
     CDS             complement(14895..15347)
                     /locus_tag="IVK82_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP6392866.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15497..16447
                     /locus_tag="IVK82_001805"
     CDS             15497..16447
                     /locus_tag="IVK82_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP6392867.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16440..17399
                     /locus_tag="IVK82_001806"
     CDS             16440..17399
                     /locus_tag="IVK82_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP6392868.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17396..18151
                     /locus_tag="IVK82_001807"
     CDS             17396..18151
                     /locus_tag="IVK82_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6392869.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18174..19127
                     /locus_tag="IVK82_001808"
     CDS             18174..19127
                     /locus_tag="IVK82_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6392870.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19771..20067)
                     /locus_tag="IVK82_001809"
     CDS             complement(19771..20067)
                     /locus_tag="IVK82_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP6392871.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20422..20778
                     /locus_tag="IVK82_001810"
     CDS             20422..20778
                     /locus_tag="IVK82_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP6392872.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20788..21687
                     /locus_tag="IVK82_001811"
     CDS             20788..21687
                     /locus_tag="IVK82_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP6392873.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         7275 a         3877 c         4676 g         6173 t
ORIGIN      
        1 cagcttttta tccttctttt tttaatcttt ttgacacgta aaagtcattt ttctgataat
       61 tctggcgata aacagaaatt atgttgcaaa gtaaagcggt tttctatata attattaata
      121 attcattaca caaagataag aataaccttt gttataaaac gatttattct gtgtaatgtc
      181 cttttttatt aagaaaaact tccatttagg aagcagagag gggtattcga ttgtccgata
      241 accaattatt agaagttcag aacctacata caggattccg actcaaagat gactattacg
      301 atgcagtaga tgacgtttca tttactttag acaaaaacga gattttagca atcgttgggg
      361 aatctgggtg cgggaaaagt accttggcta ctacgattat gggattactt gatcctaata
      421 acacaaaaat tacaggtgag attttatata acgatctgaa tttaatcgac ctgaacgaaa
      481 cgctctataa taaaatccgc ggaaatgata tcggaatgat tttccaagat ccgttgtctg
      541 cattaaatcc tctcatgaga atcgaagacc agatcaaaga aggactttcc tatcacacaa
      601 agatgaatgc agaacagcgt caagctcgtg cactagaatt gctagaacaa gtagggattc
      661 ctaatcctgc gagagttgga cgccaatatc cccatgaact atcaggcggt atgagacaac
      721 gtgtcatcat cgcaatcgca atcgcctgca aacctccaat cgtcattgca gatgaaccga
      781 cgacagccct agatgtaacc atccaagcac agattcttga tttactgaaa gaccttcagg
      841 aagaaacaaa aacagggatc atcttgatca ctcatgactt aggagtcgta gcagaaatgg
      901 ctgatcgagt ggcagtgatg tatggcggac agttcgttga agtagcaggt gtaaaagaac
      961 tgtttgatca tcctaagcat ccttatacac gctctttact gaactctata ccacaagaag
     1021 gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt tccttctctg aaaaacatgc
     1081 ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat accagcaagc gatcatgaag
     1141 aagagcctat ccttcatgaa gtcgcaccga accacttggt acgttgctcc tgctataagc
     1201 atttccactt tagagacgaa aaaggagagg tgtagatgac agaattaatc acaatcaaag
     1261 acctgaaagt tcattaccct attcgcagtg gattcttcaa tcgagtaacg gatcatgtat
     1321 atgctgtaga tggtgtggat tttattatcg aaaaaggaaa gacatatggt ttagtgggag
     1381 agtctggctc tggaaaatct actacgggaa aagccgttgt gggattagaa aaggtaacag
     1441 ccggacaaat catgtatgaa ggaaaagatg taacgaagcg aagcaaccgc aaaaaaatgg
     1501 gttataacaa ggatgtgcag atgatcttcc aagattcaat gtccagtttg aatcctaaaa
     1561 agcgagtgtt ggatatcatt gctgaaccca tccgaaattt tgaacggtta agcgatcaag
     1621 aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg gatgccggaa gatgcgctat
     1681 ataaatatcc tcatgaattt tcaggcggac aacgtcaacg tctaggagta gcccgtgctg
     1741 ttgctaccaa tccaaaactg atcgtagccg atgaacctgt atctgctctg gatctatctg
     1801 ttcaagcgca agtattgaat ttcatgaaac gaatccagca agaatacggc ctcagctatt
     1861 tattcatttc acatgattta ggtgtagtaa aacacatgtg cgacaatatc gcaattatgt
     1921 acaaaggtcg atttgtagaa atcggtacaa gagaagacat ttataatgac ccgcgacata
     1981 tctatacgaa acgtctgctt tcagctattc cgcaaatcga tgtagaaaat agggaaagcc
     2041 acaaagaaaa tcgacgaaga gtagaacaag agtatatcca aaaccaaaaa gaatactatg
     2101 atgcgactgg tcgagtctac gatttgcgta cgatcacaac tacacacaaa gtggcgttga
     2161 aagatggagg tgcaagctaa tgtggaaaac gattctgcga cgtgttttat tgatgatccc
     2221 gcaagttatt attttaagtg ttctgatctt cttattagcc caagcgatgc cgggggatcc
     2281 atttaccggt ttgatcaatc caaatcagga tccggcagtg atcgaacaga tgcgacaagc
     2341 agctggcttg aatgacccat ggtatgtcca gtacttccgt tggatcggaa atgctttgca
     2401 aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt gctgacttga tcggcgaacg
     2461 gatcatcaac actttatatc tatcgatctt gacagttgtc cttacttatt tgattgctgt
     2521 gcctctgggg atgcttgctg gccggtatca aaattcaatc ttggataaga ttgtcgtaat
     2581 ctacaacttc tttagttttg ctgttccttt atttatcttc ggcttgatca tgctatttgt
     2641 ttttggttat cgtttaggct ggttcccaac aagtggatca caaacactag gtttgcaagg
     2701 tggatttatc actcaatgga tagaccgttt gaaatatatt ctgttaccat caattaccca
     2761 agcatttttg gcgactgccg tcacaatcca atatctgcga agtgaagtaa tcgattcgaa
     2821 atcgctcgat tttgtcagaa cagcccgttc caaaggggta ccgacgaata aagtattcag
     2881 tcgacatatt ttccgaaatg cagcgttgcc gatggcttct caaatggggt atgaaatcac
     2941 ttcgttgatt gctggttctg tagttatcga aaagattttt ggttatccgg gagtcggaaa
     3001 actatttatt gattccatcg gccaacggga ctataccgtg atcacatcct tagtattgat
     3061 tttaggaatc gcgacgctag tggggacatt actttcagat atcatcatga gtatcgttga
     3121 cccgcgtatt cggattcaat aaaaccaatc agaaaggaga atggatcgat gagtgaaaaa
     3181 aacaatacag cagttcaaga aagtattcct cctatgggga tacgaatgat tgctcgagaa
     3241 tttaaaaaag ataaaattgc tattttttca ttaggtttgc tagtggtttt attattagtg
     3301 atctttatcg gtgcgatgct gaccgatcaa gacaaagtga tgtatgttag tatcttcgac
     3361 aaatacgctg caccaggaac agtttcgaca caaggacaaa agttcattct gggagcagat
     3421 gaaggcggac gtgatgtatt aggacaattg atcataggtg ctagaaactc tgttacgatc
     3481 ggatttgcga tcacgatcct tacttcgatc atcggggttg gattagggat catctctggc
     3541 tactacggag ggatcattga caacatcttg atgcggatcg ttgattttat catgattcta
     3601 ccgatcatgc tgatcattat cgtcttcgta tcgattattt ccagatacaa tgtctggtcc
     3661 tttatattca tcatgagtgc attttattgg gtagctaaag cccgattatt ccgaagcaag
     3721 acattatctg aagcaagaag agattatgtc agtgcctcaa aaacaatggg aaccaatgat
     3781 tttaaaatca tgttccgcga aatcatgccg aatctaagtt cattaatcat tactaatttg
     3841 acgatgaact ttgcggcaaa catcggtatc gagacaactt tgactttctt aggtttcggc
     3901 ttgccggcaa atgtacctag tttaggtaca ttgatcggtt atgcaagtaa cggagaagtc
     3961 ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc ttattttagt tttgatgttg
     4021 agtatcaatt atgttggaca agcattcaag cgttctgcag atgcgcgcca acggttaggc
     4081 tagtatagaa gcgaaggggg aaataggaat gaagaaaacg atttttggat ttgcagcgct
     4141 gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt acagatacct ctggaaacaa
     4201 ctctggaggc aacggcaata acaaagagga attaactttt ccaattgcaa caaaaaataa
     4261 tgatgatgca atcgaagacg gcgaattaga agctgcggtc gcaacggaca gtcagtttaa
     4321 aggattgttc cagtgggagt tttaccaaga ttcatatgat gcagctttta tggctccttc
     4381 tcatgaaccg ctatttgcaa acgatgacga tttcagaatc acagacgacg gagctgcaac
     4441 gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa gtaaaagaca acgtcaaatg
     4501 gtcagatggt gaagatgtaa cggcggacga tgtgattttc ccttacgaaa tcattgggaa
     4561 taaagattac actggtatcc gttatgacga cactttccgt aacatcgtag gtatggaaga
     4621 atatcatgat ggaagtgccg acacaatctc cggaatcaaa aaaatagacg acaaaacagt
     4681 tgaaatcgaa tataaagaag taaacccaag catgcttcaa tcgggtggtg gtatctggtc
     4741 atatgcagca ccaaaacata cgttagaagg tgtagcaatc aaagatatgg aatcaagcga
     4801 tcaagtacgt aaaaatccag tgacttttgg cccttattac atgagtaata tcgttgcagg
     4861 agaatcagta gaatacttgc caaacgagca ctactggaat ggtacgccta aattgaaaaa
     4921 gatcacgatg aaatctgtac cgatggcaag tgcaactgaa gcattgaact ctaaattata
     4981 tgatatgatt ttccaaatgc caacagatac ttacgatacc tataaagaaa ttgctggcta
     5041 tacaaactta ggccgtgaac aaacttctta tacttatcta ggattcaagt taggtaaatg
     5101 gaatgcagat acaaagagtg tggaatacaa tccagattca aaaatggctg ataaatcatt
     5161 acgtcaagca atgggctatg cattagataa taatgcagta ggtgaacgtt tctatgcagg
     5221 tttgagaagc aatgcgactt cattgattcc tccagtattc cagtcatacc atgatgatga
     5281 gatgaaagga ttcacacaag atctagacaa agcgaaaaaa ctattagatg atgctggcta
     5341 caaagatgtg gatgacgatg gtttaagaga agacaaagat ggaaataaac taacaatcaa
     5401 ctttgcttcg atgtccggtg gggaaacagc gcaaccattg gcagattact atatccaaca
     5461 atggaaagaa attggtttgg atgtccaata cacaacaggt cgtctgatcg aattcaattc
     5521 tttctatgat aaattagaaa acgatgatcc tgaaattgat gtttaccaag ctgcttggaa
     5581 tacaggaact gatccaaatc caagtggact atggggacca aatgctgcgt tcaactacac
     5641 tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat atcagttcaa gcaaagcatt
     5701 tgatgaagag taccaaaaag aagcatatca aaaatggcaa caatatgcat ttgaagaagc
     5761 atttgcgatt ccaacgttgt tccgtaatga agtattgcca gtcaacgacc gtgtcaaaga
     5821 ctgggattgg tcttatgatg taccaaaccc atgggcaaat gtttctgtta cagcagattc
     5881 acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt cctttttcgg ttttttgata
     5941 tttttctgtt tatttctcta atattcaggt caaggcgctg atttcatgac ttctccaata
     6001 aacggtgttc aaagtccagt ttctcggtat taagtcaaat ttatgcaaaa atatggaaag
     6061 ccattttcac aaaatttttt cttcatactc gaaactaaac gaatttttca caacttctcg
     6121 tcaatcacta ggattattaa agctttactt accttttgac cgaaccattt caaatttgac
     6181 aaaaaaccag tataataagg aagtatcgaa aaaatatcca taaaaggaat gtggaaaatg
     6241 gacaaccagc tgacaaaaga actaaaagaa aactataaca tcgtttttca cgatctaaat
     6301 ttgcttgaac aagcttttac ccattcatcc tatgtgaatg agcatcgcaa tctgcaatta
     6361 tcggataatg agcgacttga atttttagga gatgccgttt tagaactgat ggtttctcag
     6421 tatttattcc gtttatatcc tcatttgcca gaaggaaaat tgacaaaaac acgagctgcg
     6481 atcgttcgcg aagacagctt gtcgaaattt gccaaagaat gtcactttga tcaatatgtt
     6541 ttgttgggca agggagaaga gaattcagga ggacgtacgc gaccagcatt gctttgcgac
     6601 ctattcgaag cttttttagg cgcgttatat cttgaccagg gattcgacac gacccatgca
     6661 ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg ctttttcaca tgagatggat
     6721 cataaaacaa aactacaaga agtcttgcaa aaatcaggtg atgtatcaat cgaataccga
     6781 ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt ggatcgaagt ttatgtagat
     6841 gatcaattga ttggcactgg acaagggaaa tcgaaaaaat tagcagaaca agctgcggcg
     6901 gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa gctttgtatt tgaaacgaat
     6961 tgaaatagcc ggttttaaat catttgcaga caagacagtc attgattttg aaaatagcgt
     7021 cacagcagtg gttggaccaa atggcagcgg aaaaagcaat attacagagg ctatccgctg
     7081 ggtattagga gaacaatctg ctaaaagtct gcgaggcgga aaaatgccgg atatcatctt
     7141 tgccggttcc gatacccgta agcagctgaa tattgcagaa gtcacagtga ttttagacaa
     7201 tagtgatcat tatctgccat tggagtacaa tgagatcagt gtaacccgcc gttatcgacg
     7261 gactggggaa agcgagtttt tcatcaataa acaaagctgc agattaaaag atatccaaga
     7321 attgttctta gattctggat taggaaaaga atcgttctca atcatttctc aaggaaaagt
     7381 agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg atctttgaag aagcagctgg
     7441 tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag aagctttttg aaacagaaga
     7501 caacctcagc cgcgtccaag acatcatcca cgaattggaa gaacagttaa ctcctttggc
     7561 cgcccagagt gaggcagcaa aggagttttt acggctaaaa gaaacactga ctcaaaccga
     7621 tgtctctttg atggttgctg aaatcaagac agcaaaaaaa gattgggaca acaaacaagc
     7681 acagcttgcg aaattcaatc tagaattagg caaactatct gagtcgatcc aagaacaaga
     7741 atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc gatcgtctga tcgaaaaaaa
     7801 tcagcaagta ttattagatc tttcggaaaa actgaagcag acagaaggac aaaaagatgt
     7861 tttgcaggag cggacaaagc atacacaaaa aagctcgcaa gaatatcaaa cttcattagc
     7921 agaagcacaa aaaaaggtta aacattttga aaagcttcaa gaaagcttga tgaaagcagc
     7981 agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat ctcataaaaa cacagcaaga
     8041 attagaaaaa taccaaaaat caacgaaaga attattggca gaattaagag atcaatatgt
     8101 tgatttgatg caagaacaag cagctgtagg aaacgaactg aagtatctag aacgtcaata
     8161 tatacaagaa acagcaaaaa gcaagcagac gttagccaag caatcggaag tagaagcatc
     8221 agtagaccga ttgatcttac aaaaagaaga attgacacaa aagcaggcgc agctaaaatc
     8281 atctttaact gaaacaaaag agaagttaga aatgatccaa caaaacggga aaaagttcca
     8341 agaagcatta gcaaaagaac agcctaaaat gtatcaatta atgaatcaag tccagcagct
     8401 acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac tattttggtt tttaccaagg
     8461 ggttcgattg gtcttgcagc ataaacagca gctttcgggt attgtcggag cagtggctga
     8521 actgatcgat gttcctgcag atttcacgtt agctatcgaa gcagctttag gtggggcagc
     8581 ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa gccatcacgt atttgaaaca
     8641 gcaaagagga ggaagagcaa cattccttcc actgacgaca atcaaaccaa gacaattacc
     8701 ggctcatata ttgacacaag cagcagctgt agaagggttt atagggattg ccagtgaaca
     8761 agtcagttat cccgatcaaa tccaaactgt ggttcaaaac ctgctaggaa cgatcctttt
     8821 agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg atccgctatc aatatcgagt
     8881 ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc atgacaggtg gagcgaataa
     8941 acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa ttgaaacagt tgactagtga
     9001 atttgaagaa gcagataaac aactccaagc acaagaaaaa aaagttcagg agctgcaaca
     9061 agaaacagct cgtttagccg aagaacaaga agttttgcgt acacgaggcg aacaattacg
     9121 ttttgaagaa caagaagcga caaatcagct acaaaacatc ataaatgaac ttgaacgttt
     9181 tgaaaaagaa aagcaaatct caacttttga gacacgtgaa ctgcagcagt ttatcgaaga
     9241 ttacgaaaag caaacgaacg aattgaaaga caaacaaaca gatcttgaat cgcaacgaca
     9301 aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc gaccagatgg aagccagaag
     9361 agcacaagta caaagtcaaa aagcacaaga acaagcagat cttgctgtct tgaaagagca
     9421 gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc caaaaagcag aggcaaccga
     9481 acgtcaagaa gcgatagaaa agcagttggc gacattgaca gcagactttt ctgatcatga
     9541 agtcacagaa gaaagtttgg aaaaacaaat taacgaatta tctgctcagc gagaaacatt
     9601 aaaagccgag ttggcaaaag ccaaagagca gcgagataga aggcaaaagg agatcgataa
     9661 gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa gcacgactgt cggaacaatc
     9721 caaattagaa gtccaaaaag accgagctga aatgatttta gataatcatt tgacctatct
     9781 gcagtcagaa tatcagctga catttgagaa agcttcccaa gactatcaag aaacaaccga
     9841 tatcgaagac agtagaacaa aggtttcatc cttaaaagaa cagattgaaa aactgggacc
     9901 agtaaactta aattcaatcg aacaatacga acaagtcagt gaaagacata cattcttagc
     9961 aacacagcga gatgatctgt tagcagcgaa aaaccaattg tttgaaacga tggatgagat
    10021 ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa gctatccgtc aagaattcaa
    10081 aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa ttagtactga cggaaccaac
    10141 tgatttgctg aaaacaggta ttgagattga agtacaacct cctggcaaaa aactgcaaag
    10201 tctaagtttg ctttctggag gagaacgagc acttacagcc atcgcgttgc tcttttccat
    10261 tatccgtgtc tgtcctgttc cgttttgtat cttagatgag gtagaagcag cgttagatga
    10321 agccaacgtg aaacgttttg gtcgttactt gagcgatttc caagatgaca cacaatttat
    10381 cgtagtcaca caccgcaaag gaacgatggt cgcagcagat gtactgtacg gagtgacgat
    10441 gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg gaagatatca atgaagaggc
    10501 gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca aattagtagc aatcgatttg
    10561 gacggtacat tattaaatga acaaaagctg atttcagatg agaataaaca agcattagca
    10621 caagcaaaac agcaaggggt caagatcgtt ctatgtacag gacgcccctt agctgcgatg
    10681 gcgcattatc tgcaagaatt aggactggta gatgaaggtg actacagcat cactttcaac
    10741 ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg aaaagaaagt gatggaagta
    10801 tcagatatcc aacgcttata caaactggct cagcaattgg atctgccact agatgttttg
    10861 tctgacaatg tggttttaca gctgcctagt gccccaagga agcaatcctt gtacaatgtg
    10921 ttgaataagc tgctgcaatt ccaaccagcg gatataagcg atattacaga tgaatttgtg
    10981 cttaataagg cagtaatcgc ttatgaccaa gaagaattag atcctaaaat caaagaaata
    11041 ccagcagaat tccatgaaat gtatgagatc atcaaaacaa gaagtatgct tttggaattt
    11101 atgccaaaag gtgttacaaa agcttatggt atttctttat tggcaaaaga tcttggtttg
    11161 aaacaagaag aaatcatggc gatcggtgat gaggaaaatg atttaccgat gatccaatat
    11221 gctggactag gcgtagcgat ggagaatgct gtgcctttcg tcaaagaagc agcagatttc
    11281 gtgacagcca gcaatgaaga aaatggcgta gccaaagtga tccaaaaatt tgtattagag
    11341 tagggggagt caaatgggat tttttgataa aatcaagcgt gcttttactg gcgaagagaa
    11401 aacagaagaa gttgaagtac aggagaagta cgacaagggt ttagaaaaga cgcgtaaaac
    11461 atttggtgaa cggatgaatg agttgtttgc caattttcga acagtcgatg aagacttttt
    11521 tgaagaatta gaagaaacac ttatcggtgc agatgtaggt tttgaaacag cgatacgaat
    11581 caccgaagca ttacgtcaag aagtgaaact cagaaatgcg aaaaaatcgg cagaagtcca
    11641 aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg gaaggaatca atgaagtaaa
    11701 tgaactaaat atccaagaag acggactaac agttattttg tttgtcggtg ttaatggtgt
    11761 agggaaaaca acaagtattg gtaaacttgc ccatcagttc aaaaacgaag gtaaaaaagt
    11821 attgatggca gcagcagata ccttccgcgc tggagcaatc gaccagttag ttgtctgggg
    11881 cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga ggcgatccag cagctgtcgt
    11941 attcgatgct ttggaaagag caaagacaga gcaggcagat gttttactag ttgatactgc
    12001 aggccgactg caaaataaag tgaacttgat gaacgaatta gaaaaaataa aacgagtaat
    12061 ccagcgggaa ttaccagaag caccccaaga aacacttctt gtattagatg caaccactgg
    12121 tcaaaacgcg atggttcaag caaaacaatt caaagaaaca acagatgtga ccggtcttgt
    12181 tttgacaaaa ttagatggta cagcaaaggg cggtatcgta cttgctatcc gtaatgaatt
    12241 gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc gatgacttgg agccgtttga
    12301 tccaaatgat tttgtcgttg gattgttcaa aggattacta aaagaagaat aacccatgtc
    12361 taaaaagcag gagctgtgac aaaagttgca gctccttgtt tgaatgattg tttttaacat
    12421 agctgtgttt ttgatgcctt catttttata cttatttgta agcggttctc aaacaatttc
    12481 gttctttttc ttagactttt tgattataat tagattcata tgaattggag gtcacttaat
    12541 gatgaatttt aagcaattat tattgcatgt caatgcgcgc ccttttatcg accaagctcg
    12601 atttggtata gaacgggaag gacagcgggt tgatcttgca ggaaatctag caaaaaccga
    12661 tcatccagca atctttggcg atcgatccta tcacccctat atccaaacag attttagcga
    12721 aacacaaaca gagatgatca cccctgttac cgattctatt cccgaattat ttcagtatct
    12781 cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa gaagagatga tctggccatt
    12841 aagtatgcca cctgccttac cggaaaaaga cgaagagatc attattgcaa aattaaaaaa
    12901 tttcgaagat gtcttgtatc gacgttattt agcaaaagaa tacgggaagc gaaaacagat
    12961 ggtaagcggt attcatttca attttgaatt tggcgatgag ttgctaagaa cattgttcag
    13021 ccatcaggaa gaatttcaag atttttccga attcaaaaca gaactttatt tgaaaacagc
    13081 gagaaacttt atgcgctatc gctggatgat cacttattta ttcggtgctt caccaatgag
    13141 tgagaagaac tactttttag atgaatcaca tccgcaagaa cctgttcgca gtattcgcaa
    13201 cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt tcctatgctt ctatgaaaca
    13261 gtatttagca gatatcgagc gaatgatcga agaagggaaa ctttcggagg agaaagaatt
    13321 ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca gatttagcaa caacaggtgt
    13381 tcgttatatt gaattgcgga atatcgattt gaatccttat gcaagattgg gtatcaatcc
    13441 agagcaagtt cggttcttac aattattcct gatgtacatg ttatggacag aagaaaaaga
    13501 agactgtgac caatgggtag cagaaggtac gactcgaaat aacaaagtag cactagaaca
    13561 accatcagat caaacagaat ttcatcaaga aggtagagaa attcttgaag gaatgaagca
    13621 aatgctggtt gaattggatt ggttggattc tctttattta gtagaggaag cattgactca
    13681 aatggatcat cctgaacaaa cgttagcagc gaaactctat caagaagcac agctatcgag
    13741 tcagcaagaa gttgctgtgg cattgggaca tcaatattat aaagaaagcc atgaacgtcc
    13801 ctatcagttg gccggttttc gcgaaatgga actttctact cagatattta tgttcgatgc
    13861 catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg gatcaatttt tgcgtctgca
    13921 attccaagac catgtagaat atgtgaaaaa tgcaaacatg acaagtaagg acagctatat
    13981 cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa gtcttaaaag aagctgggtt
    14041 ccgggtacca ggaggcgcag aattttcatc catggaagaa gcggtaaaag cttatccgag
    14101 atttgccgag caggcatttg tgatcaaacc taaatcaacc aattacgggt taggtatcac
    14161 gattttcaag gaaggagcta gtctggaaga ttatcaagca ggtttagcca ttgcttttcg
    14221 tgaagacagt tcggttttag tagaagaatt tatgccggga acggaatatc gtttctttgt
    14281 gatcgacgga gaagtccaag ccatcatgtt gcgagtccct gctaatgtta ttggtgacag
    14341 tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt gaccctttgc gtggaacgaa
    14401 tcatcgtgca cctttagaat tgatccaatt aggtgaatta gaacaattga tgttgaaaga
    14461 acaagggttg acgatcgaat cagttcccca agccaatcaa attgtttatc taagggagaa
    14521 ctcaaatatc agtacaggtg gtgattcgat cgatatgact gatgaatttt ctgaagctta
    14581 caaaaaaatc gccgtttctg cggtagaagc actaggagct aagattagtg gtatcgattt
    14641 gattatacca gataaagaaa tcgatcctac aactgataaa aaagcatatg ggatcatcga
    14701 agcaaatttc aatccagcca tgcatatgca tgtttatcct tttgcaggaa aaggcagaag
    14761 attgacgatg aatgtattga aattgttata tcctgaagta ttttaaaaga aaaagctttt
    14821 gacagattgt cctatttgat gtcaatctga tcaaaagctt tttttgcatg taaaaattta
    14881 tgtgtttttt cgttttaaaa taaacgatcc cagaagcttt ttttcttttt tggtttttct
    14941 ttttcagctg gcgaagagtt ttcttctggg tatttttttt cgatatcaat cacaggttca
    15001 tttactgctt cttttgctgt taagagcaat ccgaattcct caggatttgc agtgacgtaa
    15061 tcgttgacta cagtgaaacg gctattgttt ttcgttgcta gctggatata ttggttttgg
    15121 acagaaaggg gtagagcccc attcaagagt atggaagcat ctggatgttt tttgagttct
    15181 tttaaaaagt tttctttgtt ttttggttgg atcatttcag caacagtcat actaagatag
    15241 cagcgttccc tgaatgtccc taaatattta tgctgttctt cagggttcac taaaggtgta
    15301 ccataaagtc ccttatccaa atggcttttt acatcatctt gtgccataaa aaataccctc
    15361 cttcttctct ttgtctttct tacgattata gcatattccc aataatacct aaaacatatg
    15421 aaaaagcggt tgcttttgtt caacgattca cttaaaataa gaatgaaaat cattctcaat
    15481 tagaaaggtg gattggatgc gctacggacg actagtagca ttattagtta ttttaagtag
    15541 tatttcattg ttcatcggag tatcgaatgt acaattgtca gaactgctcc agttagatgg
    15601 gcaaagctta caaatcttgc tagtgactcg tttgccacga acgatttgtt tgatccttgt
    15661 gggagcgacc agtagtattt gtgggttgat catgcaacat ttgacccaaa ataaatttgt
    15721 ttcaccaacg actgcgggca cgatggatag tgcgcgttta ggtattttag tggctatgat
    15781 tttcctacct ggtgcatcct tgattgtccg atccttgaca gcattttgct ttgcttttgc
    15841 aggaacattg ctttttttat cccttacgcg attgttcctc cagaaaaatc aattgatgct
    15901 tccgttgatt ggagtcatgt tagggaatat cattggttca gttgctactt tttttgctta
    15961 tcaattccag ttggtacaaa atatgacttc atggcttcaa gggaattttg cgactgtgat
    16021 acgaggaaat tatgagttat tgtatgctgt tgttccattg ttgctcttgg tttggctatt
    16081 tgcttaccga tttacgatca ttggtctggg cgaatcgttt gcagtaaatc tgggaataga
    16141 ttaccagaag atgcagtttt taggcattgg cttagtatca ttggctagtg cagtcatgtt
    16201 gatcatggta ggaagcattc cttttatcgg agtgatcatt ccgaatcttg tctctagaat
    16261 atatggagat caagtgcaca aaacaattgg cgtaactgct ttagcaggta gtttgttctt
    16321 agtattttgc gatcttgtcg ctcgcgtgtt gatttttcct tatgaaatac cagtcagtgt
    16381 gatcgttgga atcatcggtg gggctatctt cctttatctt ctgataaggg gacgacgata
    16441 tgcttaaaaa aaagccatct atttttattg gtatgcttgt tcttgtttta tttgttacag
    16501 gaatgtactt gacgatccat acatatggaa actggcattt tgcatgtgca ttgaggagta
    16561 agaaaatttt tgcatttatc cttgtctcgc tagctgtcag cagtgccaca atcagctttc
    16621 agacaattac gcataaccag tttttgactc cttcagtatt gggacttgag tcgttatatg
    16681 tccttgtcca aacggtcctt ttcttcttta tcggaggagt gacgatgctt cagcagacag
    16741 gtacagtttt tttcttattg aacgttgggc tgatggtcgg gctgagtcta ttgttatttt
    16801 tctttctttt gaaaaaaatg agagcgaatc tttttttatt actgatgtca gggatgattt
    16861 taggtacgct ttttggtagt gtcagtacct ttttacaagt catcatggat ccgaatgaat
    16921 atgatttgct tcaaggcaga ttatttgcaa gctttggcaa tatccagagt cagtatcttt
    16981 tgcctgcttc tttgatcatt atcggctgct ggtacctgtt atttcgatat cgacatgtgc
    17041 tagacgtttt ccatctcgga aatgaacaag cagtaaacct cgggatagac acagatcgtt
    17101 ttgaattttt gattttatgt tgtgtcagtc ttttgacagg gacatcaacg gctcttgtgg
    17161 gtccagttac ttttttaggg tttattgtcg caaatatcag ttatcgtgtc ttttctactt
    17221 atcgtcatgg tcagctattt atcggaagct ttctggtagc tatgctgttt ctgacaggag
    17281 gacagctcgc agtagagcag attttcggct ggaatacaac agtgagtgtc gtcgttgaat
    17341 ttgtcggagg aatttatttt gtcacaaaat tgattcgcga aaggaagagt atcctatgat
    17401 tgatttacaa aaggtcaata aactgtacca tggaaaatca gtggttaaag atgtacagtt
    17461 aacgattcct gaaaagaagt tgactgcttt tatcggtcca aatggtgctg gaaagtcaac
    17521 ggtgttgtcg atgatcagtc gtttgatacc aaaagataca ggtgaaatct atttagacca
    17581 taacgaagta aaggcttgga aatccaatga attagccaaa aaattatcga tcctacgaca
    17641 atcgaatgaa ataaatgtcc aaatcaccgt gaaagaatta gtagaatttg gacgatttcc
    17701 ttatacgaaa ggaaaattga cgacaaaaga cgaggaaatc gttcaggaat cactggaaca
    17761 cattgggtta gaagaaatag cggatcaatc gattgacact ctttcaggcg gtcaactcca
    17821 acgtgcgtat attgcaatgg tgctagcgca agacacggat tatattttac tggatgaacc
    17881 tttgaacaat ctagatatga attttgcggt tcaaatcatg cagattttaa aaaatttagt
    17941 acaagaaatc gggaaaacaa ttgttattgt tcttcatgat atcaactttg ctgcaagtta
    18001 tgcagacgaa attgttgcaa tgaaagatgg acagttattt acgcaaggaa cgacccaaga
    18061 aatgataaaa aaagatgtat tggatgaatt atacgaaatg gacatacgga tttgtgagat
    18121 tgaaggccgc agattctgtc tttatttcta aaactaacat taggagagaa gagatgaaaa
    18181 aattagtagt aggtatcttg attctagcat catttggttt agcagcatgt ggaaatagca
    18241 acaacaccag tcaggcagac acaaaaaaaa gcagcacaca aactgaaact acattgacga
    18301 taacagatag taatggagac caaatcgagg tgccaaataa tccagaaaaa gtcgtcgtat
    18361 tcgacaatgg ttctttagat acgatggatg ctcttggtgt tggagataag gtagttggag
    18421 cagctacaag cagccttcct gagtatcttt catcctataa aaaagtagaa tcagcaggtg
    18481 gaataaaaga accagactta gaaaaaatta atcagcttca gccagatcta atcatcattt
    18541 ctggccgaca aagagatttt caaaaagatc taagtcaaat tgctccgacc attttccttt
    18601 ctttagatgc gaaaaatcca tgggaatctt tccagcaaaa tgtcactgct ttaggggaga
    18661 tctttggtaa gcaagaagaa gctaaaactc aattagagga attatctagt gcaatcgatc
    18721 agacgaaaaa gaaagcagaa gctactgaca aaaaagcact agtcacactt gtgaatgaag
    18781 ggcaattatc agcctacgga tctggttcta gattcggttt catccatgat ttatttggct
    18841 ttgaacaagc agatgatcaa atcgaagcat ccactcacgg tcaaagtgtt tcttatgaat
    18901 atgtgctgga aaaaaatccg gatatcttat ttgtagtaga cagaacaaaa gctattggtg
    18961 gagacgacag caaagatgat atttctgcca atgaactggt agcacaaacg aatgctggta
    19021 aaaatcagca gatcatctcc cttgaaccag atgtctggta tttaagcggg ggcggtttag
    19081 aatcaatgaa actgatgatc gaagatgtga atcaagcatt taaatagcat aaataacaga
    19141 ggattaaact ctcatactat gtaacaataa tgtttcttac attcttaaat agcaagagcc
    19201 tatcctcaaa gaagttatca aaaaccatcc gtaaaatcgt attgatgatt ttacggatgg
    19261 tttttattta tttgagaaat cttttttcaa cgtgaattca ttatctacat catacgggtt
    19321 gattctagaa tggggagtgg caaaaaatag tgtgatccat tttttcagca tcaagatcgc
    19381 ctcctttgat ttgaatcaaa ggtacaaagc gaaacttaga taaaacttaa atttagatat
    19441 ttttattttg taccgatctc ttctttaaac gatgttccat ctgctgcttc tcggcaataa
    19501 gtccactaga ctagaaaata tgagaagcta ttttctgtcc agcgttctta ttactcaaaa
    19561 caaatcgcag ttgtcacaat ctcttctaaa cgatgttcca tctgctgctt ctcggcaata
    19621 agcccactag actagaaaat atgagaagct attttctgtc cagcgttctt attactcaaa
    19681 gcaaatcgca gttgtcacaa tctcttctaa acaatgttca atcagctgac cttcggtatt
    19741 agctcaaagc actgatttca caacctctat ttaggcatgt gtctcagcaa gcggttgata
    19801 gttgatgatc tgcatttttc gataaaaatc ggcgggaagc ttactccacc gttttagtaa
    19861 ttgatagaaa taactctctt ctgctttgat tttatactcg cttaatatca atccaaaact
    19921 attcgttttt tttgaagcta attttttata aatatccaat gctttggttt tatcttcatt
    19981 gacaaagagt gtttttactt caatataaga accctcgata ttttgtgttt cttcgataaa
    20041 atagatgttt tttatatatt ccttcatagt gattcctcca aagaagtctt ttatttgata
    20101 agtttactct accgaagagt tgttaataat ccgtgaacaa aaagtaattc tttttccaat
    20161 agttgttaaa ggttttttat tttttatagt attttttgtt atttaagaaa ggaataaggt
    20221 taaacaaata acaggttgct ttttgggtct ttttccgttc aagaaactga acgaagaaaa
    20281 gggaaagttg tctcttttgt gataagagaa atcataataa acgaatgact tactttccaa
    20341 taaattgaga acagtcagaa ccagacaaat tagttgcatc tgatgaataa agtgattatt
    20401 ctatgacatg aggtgataca gatgaaatta gaagtgacaa aagaagcaca agaagtattg
    20461 aagaataaat tagaagcaga cgatcaactt ttattagata tcgaaaacgg tgacggtcca
    20521 tttgcagaaa gccaagtcag ttgccaattg gatacggcat ttcgattgat cattgtcaaa
    20581 aaagatacac aaacagatct aagtctttat tctgtagtgg ctgatacacc agttggacca
    20641 atcaaaatca aagacagtgc aatactttac atggatgatc catccacact tgctttagaa
    20701 ccaacttata atagtttaca attaaaagga ccaagcggct tgagaaaagg caacttgcaa
    20761 gttgttcgaa aagactagag ggataaaatg ggaaaattgg cgatcatcag cgacctacat
    20821 gtggacatca ataaattcgg cgaatcagag ctgatgcagc tttggcaagt tttgcaaaag
    20881 cacaagatca ctcggcttca tctagccgga gatacggcta ataaagtaga ccgttgccta
    20941 gctgttgtcg aatttttcgt caataaagga ttatctacga catttcattt cggcaatcac
    21001 gagttggcag atgtgcccaa tgaacagatg atggaccatt ttccagaccc acatttttta
    21061 aatgaacggt atattgcttt aaatgagcag accgttcttt tagggatgaa tggctggtac
    21121 gactatcagt tttccgaatt gaaagattac gaggcaatcg tacgaatgaa gcgtttgtat
    21181 tggtatgatc ggttcatcca gcgtgggaaa acagatctcg aggtcaatgc agaaatgctt
    21241 gcagctgcaa aaaatttgct agaccagctg gaagcaaaaa aacttgaggt gattttatcc
    21301 actcatttcg ttcctaaacg ggcatttatc atgtatcaat ctgctcctta tgagcgttgg
    21361 aacaaattaa atgcattttt aggatcagaa tcattcggag aattacttga ccgttattca
    21421 aatatcaaac aagtagtttt tggccatacg catcgtcgct ttgaagataa atccatcggt
    21481 cacacgattt acagctgcag accttttggt tattattatg aatggcagct gacccgagat
    21541 tttgtcttga aagaacagtt gctagacagc tatgatccta tgaaattacg tgtcctcttg
    21601 cgtgcccatc acccagcttt tcaggcctat caaacacaac atttgcaaga ggaatttgaa
    21661 caagcgatga caatcgttcc gtattgagac agcattagta atgtgtaaaa gaaaatacct
    21721 tgtaaaacat aaggataagt aagagcagta gatccattta tcgtggatat gctgcttttt
    21781 ttgcggaaaa taatcagcga atgtttattt gatcggatga tttgtaactc gcagagtact
    21841 agatgcaagc gtgtattctt tttttgaacg ataatcacaa aaaaaaatat aatatacatt
    21901 ttttatttaa ttataattaa tattgattga agtctaggca gtcaagtcga gagtgtaaaa
    21961 tattttgtgt aaatgaaaaa atccatacaa aaaaggaagt c
//
LOCUS       DACCCS010000044        21923 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.045, whole genome shotgun sequence.
ACCESSION   DACCCS010000044 DACCCS010000000
VERSION     DACCCS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.045"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            282..521
                     /locus_tag="IVK82_001812"
     CDS             282..521
                     /locus_tag="IVK82_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392874.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="IVK82_001813"
     CDS             complement(883..1143)
                     /locus_tag="IVK82_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392875.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="IVK82_001814"
     CDS             complement(1328..1825)
                     /locus_tag="IVK82_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP6392876.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IVK82_001815"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IVK82_001815"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP6392877.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IVK82_001816"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IVK82_001816"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP6392878.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..5209)
                     /locus_tag="IVK82_001817"
     CDS             complement(4547..5209)
                     /locus_tag="IVK82_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6392879.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5219..6034)
                     /locus_tag="IVK82_001818"
     CDS             complement(5219..6034)
                     /locus_tag="IVK82_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6392880.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6296..6739)
                     /locus_tag="IVK82_001819"
     CDS             complement(6296..6739)
                     /locus_tag="IVK82_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP6392881.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6873..7211)
                     /locus_tag="IVK82_001820"
     CDS             complement(6873..7211)
                     /locus_tag="IVK82_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6392882.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7199..7576)
                     /locus_tag="IVK82_001821"
     CDS             complement(7199..7576)
                     /locus_tag="IVK82_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6392883.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7800..8993
                     /locus_tag="IVK82_001822"
     CDS             7800..8993
                     /locus_tag="IVK82_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6392884.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9159..9581)
                     /locus_tag="IVK82_001823"
     CDS             complement(9159..9581)
                     /locus_tag="IVK82_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6392885.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9976..10170
                     /locus_tag="IVK82_001824"
     CDS             9976..10170
                     /locus_tag="IVK82_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392886.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            10167..10661
                     /locus_tag="IVK82_001825"
     CDS             10167..10661
                     /locus_tag="IVK82_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392887.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(10823..11995)
                     /locus_tag="IVK82_001826"
     CDS             complement(10823..11995)
                     /locus_tag="IVK82_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6392888.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12201..13016)
                     /locus_tag="IVK82_001827"
     CDS             complement(12201..13016)
                     /locus_tag="IVK82_001827"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6392889.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(13166..13612)
                     /locus_tag="IVK82_001828"
     CDS             complement(13166..13612)
                     /locus_tag="IVK82_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP6392890.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(13609..14613)
                     /locus_tag="IVK82_001829"
     CDS             complement(13609..14613)
                     /locus_tag="IVK82_001829"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6392891.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(14653..14982)
                     /locus_tag="IVK82_001830"
     CDS             complement(14653..14982)
                     /locus_tag="IVK82_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP6392892.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(15108..17435)
                     /locus_tag="IVK82_001831"
     CDS             complement(15108..17435)
                     /locus_tag="IVK82_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6392893.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(18278..18571)
                     /locus_tag="IVK82_001832"
     CDS             complement(18278..18571)
                     /locus_tag="IVK82_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP6392894.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(18974..20152)
                     /locus_tag="IVK82_001833"
     CDS             complement(18974..20152)
                     /locus_tag="IVK82_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6392895.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(20256..20570)
                     /locus_tag="IVK82_001834"
     CDS             complement(20256..20570)
                     /locus_tag="IVK82_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6392896.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            20677..20892
                     /locus_tag="IVK82_001835"
     CDS             20677..20892
                     /locus_tag="IVK82_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392897.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(21192..21434)
                     /locus_tag="IVK82_001836"
     CDS             complement(21192..21434)
                     /locus_tag="IVK82_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6392898.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(21421..21849)
                     /locus_tag="IVK82_001837"
     CDS             complement(21421..21849)
                     /locus_tag="IVK82_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6392899.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         6454 a         4313 c         3706 g         7450 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
     5041 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
     5101 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
     5161 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
     5221 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
     5281 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
     5341 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
     5401 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
     5461 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
     5521 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca
     5581 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg
     5641 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
     5701 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
     5761 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
     5821 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
     5881 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
     5941 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
     6001 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
     6061 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
     6121 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat
     6181 ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc
     6241 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     6301 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     6361 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     6421 ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     6481 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     6541 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     6601 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     6661 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     6721 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     6781 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     6841 tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc
     6901 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     6961 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     7021 cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg
     7081 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     7141 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     7201 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     7261 gtaaacgttc caaacactct tgcatttcag caatttcttt cgtcaaaagt tcactttctt
     7321 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     7381 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     7441 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     7501 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     7561 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     7621 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     7681 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     7741 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     7801 tgacaacgta tcagcgaaag gtattattat cgacatcggc gggaattgct ttagagaata
     7861 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     7921 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     7981 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     8041 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     8101 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
     8161 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     8221 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     8281 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     8341 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     8401 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     8461 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
     8521 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     8581 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     8641 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     8701 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     8761 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     8821 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     8881 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     8941 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     9001 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     9061 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     9121 gctttttcaa ctgctaattt ccttctatct ggcggcgact attctttgac gtccgtcatt
     9181 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     9241 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     9301 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     9361 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     9421 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     9481 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     9541 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     9601 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc
     9661 aatagtgcat aaacctgtta ttatttctct ttttttccta agtattctat tgatatgaat
     9721 catttaatcg tttattatat aaatgtcaat aatctaataa taaaataacg tacatatttt
     9781 attattgcat acttaataac agaactatag caaaaaatcc cgtgtatcaa cacgggatta
     9841 gattaagttt cctcaatcac tattcataac acaattatta taacaagtaa gtaaacaatt
     9901 gaaaaggaca tttattgaaa acggttgttt tttacttaag gaggaaattt agcgaaaaaa
     9961 ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg ggtgtggtgc tactacgtct
    10021 actactcaaa gcacaagttc agatcaggct tccagcagca gtaccacgat ctccgaaagt
    10081 acggcagatt catcgaaaga acaagaacaa attgaaaaga aaaaagaaga gaaacaaaaa
    10141 gcggaaaaag ctgcactaga aaaaaagtga cagaagcgga atctgctttg tcacaagctg
    10201 aagcaaatcc tacaaaagaa aactatgcaa ctgcttcagc agctatccaa gcgctacctg
    10261 aaaacaaaca agagttgaca agccgtcttg ctgcaattga ttccacaatc aaagcaaaag
    10321 aagcagaaga agaacggaaa aagcaagaag aaattgcacg acaacaagca gaacaagcgc
    10381 aacaagaagc aatcgcacag caacaagcag aacaggcgag gcaagaagca atcgcacagc
    10441 aacaagcaga acaggcgcga cagcaatcag cagctgaaca ggcctctcag caggcacaag
    10501 ccgcagctcc tgagcaaaat aatgaacaaa ctgtctatgt cacaccgacc ggttcaaaat
    10561 accatacaca taaatgtgga aatggtacgt atacccctgc cactttaagc gaagctcaag
    10621 ctcgtggttt aaccccttgc tccaaatgtt atggaaacta attcaaaagt tatactataa
    10681 taataaaatt cttctgaaac ataaaaaaat agaagatggc gttccagata gtaaaccaaa
    10741 aataacagtt tacacgtcca tttcgtcagc ttctctcttt tttcatttag tctgttccct
    10801 ctgctttttt ggacatcttc attcattctt tctcaatcaa atagtacacc tctttttctt
    10861 ttcgcagttt atatcttcct tttttcaatt tcattgaaaa ctgtttcgaa tgtttatgtt
    10921 ttacatggat tttaagcagg agctcctcat gttgcaagcg atagttcctg ccttttaaat
    10981 cagaaaatct cactagtccg tattcatcag aagctttcac aaatggaact ttttctctag
    11041 taatatagtg tttgccgttg agcgttttta cagaaaagac cattccttcg attggtcgtc
    11101 cgtttgtgtt ctttacataa aaagataaag gatagtttcg cttagcgatg gccaaagaat
    11161 ctaaataagc gatgagtagc caaactaagc cgattgatcc aagtagtata ccgataaacc
    11221 aaataaaaaa tttccatttt ttccattgat caattccttg gatcgctttt tttgcctctt
    11281 ttgcttgata aggaatacga tgccccgtca caagcagtcg atgtgtattg accatataag
    11341 gagtacaggt aagcaaagtc acgagatctc tgcctttttg gatacccaaa gaatctactt
    11401 catcaggtaa gaccacttgg attttttcta cttgatacgc caatgtcttt ccattgatct
    11461 ggatgaaaaa ctggtctcct tttttcaact taggcaaatc tgtaaataat tttgcttctg
    11521 ggagtccacg atgcccagaa agaacggcat gggtgttttt cccgccagtc ggataagaac
    11581 tttcttccaa taatgacgtt cctttttgca aaaatatttc cgttgtctga tcgaatatag
    11641 gtaagctgac attgattttt gggatttcga tgactccaag catatgttcc ataaaaaagg
    11701 cattactagg taaatctttt aatgcttttg catcaactgc ttggttgaaa ctgcctatcc
    11761 ctggtgatac gttttgctct gccaactgtt ggtttttctt tgtcattttt tcctgctgtt
    11821 tttttatttc ctctgaattc ttattcgatg cttgcttttg ataatggatg atcatttgct
    11881 gggcaagaaa atcattcagt gaatcttcta caaaaggata agaaaagatc ccgatcccaa
    11941 cgactaaaat gattgccatg aagatgtcca gtattcttat tctgctttta ttcatcatgt
    12001 ccacctattt tctaatatgt atatgaagag gttgtgaaat cagcgccttg acctgagtat
    12061 tagcgaagca aacggcaaaa tagttcctcc tattttgccg tttgttgagc gaataccaag
    12121 agaccgacca cttgtcacaa cctttctccc catttgaatc actatcgttt atttatcgta
    12181 tgatttttgt ttccaatagt ttaagcttct gtttcttgat ggcgtttttt gaaatagatc
    12241 actgctcctc caactaaagc agtacctacc aagatgaata cgatgatccc cataccacca
    12301 gttgaaggca agattccttt aggttggtta tcgatcgttg tttcatcaga agttgttgga
    12361 tcatcatcta ctgtaaattc agtgtacgca gcttcattct taggtaatgc gtagccttct
    12421 ggtgcttttg tttcttgaag gcagtatgcc ccttgtttca gcccttcgat tgaaaatttc
    12481 ccttctgaat cagaaatcaa tttcacttct tcccagtcag aacctgtgac ttcatctgac
    12541 caagcatatg ggtattctcc tgaggcaagg tttgctattt ttttcattaa tttaacagtt
    12601 ggatcttttg ctcttagaat agaaaattct gcatcggcta atttttcttg cgtgttcaca
    12661 tcttgtttga tgaagtggaa atcaccgtag ttttggacat tttctacagt aatcgtgtta
    12721 tccgtagttg tattttcccg attgactaca ccgtttggat tcccaagatt ggcttcatct
    12781 gcgccatgga tgataccggt tgtttgttgc aattttccgt tttttactat aaaagtagtt
    12841 tctttttgtg gattggttaa ttgataacca tctggtgctt ttgtttcaac taaagtataa
    12901 ttgccgtcta ttaatccatt gacactgatg gatccttcag tatcagttgt gaatgtcaac
    12961 gctgactcag caggatcata gagagcgaca ccagtgtttt catcaaaacc ggtcacgaat
    13021 tgattctctg aatttttgat ctggaattgc gcccctgcta acttatctac ccctgaaaag
    13081 ttcccagctt tctggatttt gatattgctt tctttgatga catttttagc atataggtga
    13141 atcgtgtcta aaggtttgtc agggtctagg gtaagcttaa cacgatattt ggcgacgttt
    13201 cgacaccggg ctttggtgtt tctacaaaga cgtatacagc atctttccca tcagatttac
    13261 ctggaagagt caggttagct aatccttcac cagaagtcgt tacacttgcg aagggtgtag
    13321 cgtctgctgg aactgctgtg tctttacttg gatagtacgt agcagtgatg tcatatacgt
    13381 tgaattcaac gccgttcaac ggttcgctat tcggaaatgt caattccgtt ccgttgttta
    13441 tttgattttc tggtgctgaa gtgaatttca atttatgcaa attaaccgta acattttctg
    13501 gtgtaggtga tacggctgca gaaacctctg cagttcctag gccgagaacg aaaggaagca
    13561 gcatgatagc agttcctaaa aacgtccata tttttgtttt gatgtttttc attttatttc
    13621 cctcctattg tttccttttt gttgtttttt ataaaaccaa gctgtccctg ttgaaaggac
    13681 tagcaataac cctaaaattg agaaacctcc aagcatctct tcgcttgttt ttggaaagct
    13741 tcctccattt ttttcatttg atggaccatt tgtctgcatc tgaactggcg tttttccatt
    13801 attcgtcgga tttgcttttg gttcgttgat gatttccaat ggtataggat gaagcggatc
    13861 ataactgcct ttttcaactt caaagagaat ggttgattga ctcaatatat atcctttagg
    13921 cggtttaatc tccactagtt cgtaacttcc aaaagacaat ccatggacag aaaatgttcc
    13981 atctttactg gaagtaaaaa tcgttgctct tgaatcatct ttggctaccc aatgatttat
    14041 cccgttttct tgcgctaagt attggtcatt ttttttgact agaaattgag catctgctag
    14101 tttttgtttc gtttgccgat ccactttgac aaattgtttg cctcctgttt ggatggcaag
    14161 atgtgtttct actgtttgat tgtcagtcgt caaacgtaca tcgttatcaa atgtcgtttt
    14221 ttcactgttt tctttcaatg tcatttggta acggatcgtt aatttttttc cagcaaacga
    14281 actaagatct tttccagaat ctgataagac aaaaccatgt tttatttttt gtgtgtgaaa
    14341 tgtatgaatc ttgtctccac caatcaaaaa atcgatcgtt tctggttcta gccataactg
    14401 aggatcagct gtatctttga tcgtataggt tcctatctcg ttgatgttct caggaattgt
    14461 tacggtgatg agataaggga ctttatcccc atctgcaaaa tctgtctgct tattctctac
    14521 tgtttttgtt aatgtgagtg cagcaggtat tttttgttcg ctctttggat acaagtggat
    14581 ggtcgtcaat ttatgattag atgaatctgt cacaggaagc acaacgacaa atggtgctga
    14641 tttttctttt acttaatctg aagtctttat ttccgtaaat cggtaaacag cgtcacgacc
    14701 ttgctcatct ttgtccgatg cactaaaaga agcaatgccc tcttcctcgt ctactgtttt
    14761 tgtcacattt tctgccagta ttttttcact atcacttttt tgtgctagtt ttctttgtgc
    14821 gtcttccaca ctacttcctt ctgaccgcag cttgtaaaat tcatccgaga catcatatat
    14881 agcgaaagta acgccgttca acccttttac tccttgaaag ttatccggga tatttattcc
    14941 gtcgttttga ctgatacttg gcatcttatc gctttcgaac attcgattat gcaaagtaaa
    15001 tgtaataggt gattctctag ctgacacagt cggagaacct ataaaaaaga ccagtaggat
    15061 aaaaaagaga acagcaaata tatccacacg tacatttttt gtttttttca tcgctgttca
    15121 cctgcttttt tcttacgatg gataaaataa aagaagccag aaaaaataaa gatcagtcct
    15181 aaaaagacaa agggcagtag acctatccca cctgtcgaag gaagcaagag agtcggattg
    15241 atcgtcaact tagcggtatc tgttgcgata atcgtataat tattgtttac acttccttcg
    15301 cctaaactaa tcgttactat ttgtgatcca atatctgtcc ggctgttaac tgttagggta
    15361 taatccaatg attcgcctaa gacaacaccg gtaacgtgga gatttccaac cgaaatcgag
    15421 tgactttggg catcataatt aaaggcagcg tcatcgacag taatcactgc gttagcttta
    15481 tagatggtat aagttcctga tgtgttggct atatcagtaa atgacaactt gggatttgct
    15541 gctttgactt tgtctattcc gctattcgtt agtttaattg tatagctgcc agcattaacc
    15601 atattttttg ctgattgtgc atctgctgtg taatccatac tgctaagtgc cacttgtact
    15661 ggagtaagcg aactagtgac accctctgct agcttgacat ggattgacgg gacacctgct
    15721 tttccatcat atgtcttact gccactgagc tctaccgcac cgctgatttg cttaatcgta
    15781 tagacatcag atgtctgcac gatagtaaat aaatagtttg ttctggcagc tagtttagct
    15841 agtccgcttt ttgtcaattg ccaagacgta taggttccaa tttcagtagg cgcagttact
    15901 tcaactccct tttcattcat cgctttgaaa tcagttggat tccatgtgat ttgatcaata
    15961 ctgagatcat tgtttaaaag ggatgttgat tctgttttat cattaaaggc atatttaacg
    16021 atcggtacaa cagaaagacc atcatagtac ttactgccat ttgcattgat tgatactgtt
    16081 gcatttgtct tataatatag gatgacttca gaaatctgtt ttccatttgc gtcctttacg
    16141 attacatttc catttgtatc taaactagct aaaacactca ttgctgattg gttctttgtt
    16201 tctatcgctg aaacagctgt aaaggtatat ccgttgatag taggcgtatt agtcgtcagg
    16261 tcttgtgcag cacttccgta agtaagattc attattttag aaggagcaat catctgtcca
    16321 aaatagtctt gatacttaat agtgattggt gtttgtttgc ctgtataggt caccgtatcg
    16381 acgttacttt ctagggctgt gaaagaaccg tttgtttgtg caaaagaata gcccgttact
    16441 gctgttggag tgataaccac tttgtcaccc acatggattc cagataatgt agtcccgtac
    16501 gttccgattt ttgctgctcc accattgact gtataagaaa aagttcctgt ccgattcgca
    16561 ttgacctgaa ccgttgctgc ttgataggtt ggaaaatttg aaacatttcc cttaccatca
    16621 acgatctgcc catttttgat cgagtacccc gtgaatggaa tatcatttgc taactgtata
    16681 ccgattgaaa aagtactcat acctcctccg gcaggaagcc ctccccatgt tgagggtgta
    16741 aaagaataac ttttatccgc attgggtgta actgaatcga tcgtatttcc agcggtagcc
    16801 acccccatat agatactttt tgggaatgtg atagtagtac catttactgt ggagccaata
    16861 aacagattat ggatatctgg acggttatca ataaaagcga ttgctttggt tacgccttgt
    16921 aacgcagttt tatccgcact catcgatccg ttaaccgcaa caatgactcc atctttggca
    16981 tatatcactg tatatccatc tggtatggta tgtccgttga aattcgcagt ggccgcaaca
    17041 gtactaaatg tctggcttct acctgtttct gaataagctt tctcccctgc tgcttttgct
    17101 gctgtgattt ctttttctgt cggatcaatc agttgagtcg aggatgtggt tgaggttgtc
    17161 tcgttgttgc tagactgatt ggtgctttct atggactgta tttcagaata gggtggacta
    17221 tttgtctcct ctgtcgtttg gttagcaaaa tttgacgttt ctgtataaac gtttacataa
    17281 atacttgata agctccctaa gaaagtaaaa atcaagatga tcgaaatcat aatgggatgt
    17341 aagtattttc ttttagatac agctttgagt ctagaaacaa aatgtttttg acatatacgt
    17401 tggattttat tttgagtttt cttaatcatt ttcatccctc ctctcaataa aaaatcatca
    17461 aataatcctt tacacgttat tctaaattta atttttatta ttttattgat gtatagaaag
    17521 tttggggctt tttttctctt tttattctaa ggttatttta gtcttgtcct ttaatacaaa
    17581 gattttattt ttaaataaaa aatactcttt ttttacgatt attgaaaaaa aaagataaaa
    17641 cttgattaat tgaagtattg cttaaatttg gaagctatga gggaagaata aaagagttag
    17701 gacgggatta tctactattt ttctttgttt taaatatttg ttatctttga acataaaaga
    17761 gaaatgtgtc gactgcagtg ttgtgtccct catcacgaat aaacggtgtt caactagctg
    17821 atcttgtatt cacttaaaaa atggtaaagt acgagttgct attccccatt ttcaattttc
    17881 taacgctcag gacaaaatac tgttttcaca acctcattgg aataaaagca tatatattgt
    17941 ttcaagaacg aaatcttgtt atctttttaa agggcatctg tccaaaatct aattttagga
    18001 ggtacgtttt ggtatttatt cgtacaaata aaagagaatt atctttattc atgtaacgca
    18061 tccaattgat tttttcatgt acgctcctgg aacgatttta ctgacgattg cttatttaac
    18121 tgcgaatcgc tcattagctt ttatcatagc tatccatatt ttgaataacg ttcttggctt
    18181 tgtattatag atacagatga caaaaaaagt tcaagtatgc ggtgtagaac caatatcgca
    18241 tacttgaact tttttcaatg agtataaatt aaaacttcta attttcaatt cccatcatct
    18301 gatatgcccg accgtctgaa cttcttttga aaaagccaaa tgtgattaga taacgtcttt
    18361 gttcgacata atcgccacta gaaaatacgt tcagcagtat cgaattaacc tctgtttctg
    18421 tataagtcac tccatggtca aagcgtttca agacttcttt caataggatg tatttctttt
    18481 ttgttgaact tggtacttgt tttacttttc catcattcat gaatgtgtct aaaactttca
    18541 tcttttcttg ctccgtataa gtataaacca tcttctttct ccttttgatt ctaagaatac
    18601 tccatttatg ttagaatatt ctggattttt tattaaagcg gttactctta agaaagatat
    18661 tatcatagtg ctcttttttt tactactact tataggtaga tttattctaa tccattattc
    18721 tcccctacca ataattaaag aaaaaactgg atcagggtgt tcaaattaca tgctaagaaa
    18781 aaagaaccct tgaataccaa gggttctttc tcagtctgat aacaatgatt aacgacggat
    18841 ttctttgata cttgtgaaaa gtcgttaaag tattgatata aaaggatttt tattacctct
    18901 gactacatat tgactaattt gttaaaaaat cgtttttcaa gaatcctgta ataccagtgc
    18961 tttcaccgtt tttttaactc tctaaagaac taaaagcaat cagaggtttt accttttcat
    19021 ttgacgttaa gtggcgataa attttcgaag ttgtttggtc agtatggcct aagcgatctt
    19081 tcaagacttc ccagctacag tgttccgcta ctaagcttgc cattgtatga cgagctaaat
    19141 gagtatgaaa tggcttatta attcctgagc gattcacgca ccgtttgacg tgatcattta
    19201 catactctcg acgataagga acaccacgtt tactagaatc aagatacaca aacaaatatg
    19261 tttcttctat aataaacctg taatgttgcc tcatctcacc caaagcctta atcaactgtt
    19321 gtagcttttc cataactaga ggagttacta gaactcgtct agcagaacta actgtcttag
    19381 tagtttcaat tcgaaaatcc ttactcttac ctttaactgc cgtttgctta tagaacagaa
    19441 tttcatttct tttgaaatct atatctccag gctgtagagc aagcgcctcg ctgactcggc
    19501 agccagtgta aaatagaaca tggaaaagta cagacatcgg taattcctca tgaacaatgc
    19561 cataattcaa aaaagttctc aattcatcga cagtaagaaa cttgtcctca atattattta
    19621 tggcttcttt taaatcagtt accgttaact gatattttgg cattctcaca gacttcattg
    19681 gattttcact tatcaaattc tttctttgac aatatttaaa aatcatattt agaacttgaa
    19741 taagtgattg taaactattt ttggcatagc caacatttgt tccattaacg ccacattcct
    19801 ttaaggtaaa aagcaattct tgaaggcact ctggtgtcaa atcagacaac tttttatcac
    19861 caatccatcg aacaataatt tttaattgtt cctctcgctt atcataggtt atttcctttt
    19921 gttcatttaa cgaacggtaa tattggaacc attgctcacc tacaacacta aacttgtcat
    19981 tgcttgcaac aatttttttc ttaagccttt gttgtttctc catttccaga taagcgagtt
    20041 gcgcctcacg ttttgaatta aagccacgtc ttgttgttcg atatgcttga aagtaccatg
    20101 tgatcctacc agtagaactg atttgcttct tgaatcgatc tgttttttcc atttattgat
    20161 tcctcctatt ttgaaggaac ccaacaagca gaaacccttt ggaaatcttt ctatctatgt
    20221 catcattgta ccgcatctta gaaagaaaat gtaacttata aagatcgaag tagtcgcaat
    20281 ttttctcgat agtcattcga atcactcttt ctcatccatt ctaaaacatc atcacgaaaa
    20341 tagtgtcctc gaatcccgta ccaacaggga ggaaactcac caagttttac taaacggttc
    20401 agtgtactat cacttatctg aaatagtcgc tttaaatcag tatttgttaa taccagaggt
    20461 aaatgactga ctgcaagcaa tttttccgtg taatcttcca gttttttttg taaaattgaa
    20521 tagaactcta tttccagaga attaacattt gttttaactt ccaactccat cacccttctc
    20581 tctcaaaatc aaaacgacca ccacggtacc ccttaaaatt gtaaaaaaac actccagtac
    20641 tccatcaaaa cggactagaa aagaggaatt tttaatatga aacaaggaat tgcagacata
    20701 aaaataatta aagaaattct tgaaaaatct acggctaatg caatagcatt cggaacagga
    20761 atcaacttga gtacagtaaa aaaacttaaa agtggagaga gagctgaaga aaaattaaat
    20821 cttgccgatg ctattaaaat cacagaattt ggcatgaaaa atatgccaac caaaatcgaa
    20881 atatggaaat agtagttttt ctgatctatc agctgttttg ccaaagcaca taggaatctc
    20941 acctcccccc agtaatggcg ggccgatttg gttacggaag tatcattatc gtagtcgcga
    21001 ttctcatatt aaaatttgtc gcttaaaagc taaccttgtc atggcgtaac tagagaatca
    21061 aaatctacat gtactgatag atttgctatt catttgtcaa agagcgctag gctattttca
    21121 gcctatggaa atcctttatt ttgacccgat aaaaaacttc catagaatga aaaggaaagg
    21181 gaaaaaatac tttaacgagt tgtatcaaat tcaagtatct tctcaatcaa ttttgtttcc
    21241 agttgtctac gcatatattc atctacatac ataaccattc ctttttcttc ttcataaact
    21301 ggtttcatgg ctaaccgtga aatataattt tgatattttg acagaacgat ggacatggca
    21361 attgaatcac cagaagctgc ggctttgatt gtcgttagtg gtatgaatgg tatatatcgt
    21421 ttattttgct tcatatttga cacccaaata ctttcctagc tcttccaaag ccatttgtcg
    21481 ttggtaccaa acactgccga gacttttccc aataatatcg ccaatttcac gatcattaag
    21541 accgatgaag aaggacataa aaatgatttc ttgtctttga ctaggtaatg ctgcaatcgc
    21601 atttgccaac tcctcattgg aaataaagat tttcatacca aggacaataa aagtaccact
    21661 actattcaaa tttggttcca aataatagaa ctctgtacct aacccttttt gttccaatat
    21721 ttccattgat atttgccgtt ggcgaaatct agcgtacttc ttttgaatat ttaacgcctc
    21781 attgcagact actttcttac aaaaactgtc aaacgtatgt tgaatgtggg tttcctctga
    21841 gtcgcccata atattctccc cctcaaaaaa ttcattcaac tcgttttact tctatccctt
    21901 tccattatta ctacaatcta gga
//
LOCUS       DACCCS010000045        21502 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.046, whole genome shotgun sequence.
ACCESSION   DACCCS010000045 DACCCS010000000
VERSION     DACCCS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.046"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            2..400
                     /locus_tag="IVK82_001838"
     CDS             2..400
                     /locus_tag="IVK82_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP6392900.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="IVK82_001839"
     CDS             384..1079
                     /locus_tag="IVK82_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP6392901.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1663..2550)
                     /locus_tag="IVK82_001840"
     CDS             complement(1663..2550)
                     /locus_tag="IVK82_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP6392902.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2735..3574
                     /locus_tag="IVK82_001841"
     CDS             2735..3574
                     /locus_tag="IVK82_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP6392903.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3770..4744
                     /gene="sgrA"
                     /locus_tag="IVK82_001842"
     CDS             3770..4744
                     /gene="sgrA"
                     /locus_tag="IVK82_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP6392904.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5042..5398
                     /locus_tag="IVK82_001843"
     CDS             5042..5398
                     /locus_tag="IVK82_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392905.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5401..6558
                     /locus_tag="IVK82_001844"
     CDS             5401..6558
                     /locus_tag="IVK82_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392906.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6584..7024)
                     /locus_tag="IVK82_001845"
     CDS             complement(6584..7024)
                     /locus_tag="IVK82_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP6392907.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7184..7690
                     /locus_tag="IVK82_001846"
     CDS             7184..7690
                     /locus_tag="IVK82_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6392908.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7858..9771
                     /locus_tag="IVK82_001847"
     CDS             7858..9771
                     /locus_tag="IVK82_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP6392909.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9776..10777
                     /locus_tag="IVK82_001848"
     CDS             9776..10777
                     /locus_tag="IVK82_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6392910.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10979..11185)
                     /locus_tag="IVK82_001849"
     CDS             complement(10979..11185)
                     /locus_tag="IVK82_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP6392911.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11831..12679)
                     /locus_tag="IVK82_001850"
     CDS             complement(11831..12679)
                     /locus_tag="IVK82_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6392912.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12843..13745
                     /gene="gnd"
                     /locus_tag="IVK82_001851"
     CDS             12843..13745
                     /gene="gnd"
                     /locus_tag="IVK82_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP6392913.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13747..14748
                     /locus_tag="IVK82_001852"
     CDS             13747..14748
                     /locus_tag="IVK82_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP6392914.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14745..15029
                     /locus_tag="IVK82_001853"
     CDS             14745..15029
                     /locus_tag="IVK82_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6392915.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15062..15859
                     /locus_tag="IVK82_001854"
     CDS             15062..15859
                     /locus_tag="IVK82_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6392916.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15873..16706
                     /locus_tag="IVK82_001855"
     CDS             15873..16706
                     /locus_tag="IVK82_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6392917.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16724..17581
                     /locus_tag="IVK82_001856"
     CDS             16724..17581
                     /locus_tag="IVK82_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6392918.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17603..18112
                     /locus_tag="IVK82_001857"
     CDS             17603..18112
                     /locus_tag="IVK82_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6392919.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18149..18550
                     /locus_tag="IVK82_001858"
     CDS             18149..18550
                     /locus_tag="IVK82_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392920.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18662..19015
                     /locus_tag="IVK82_001859"
     CDS             18662..19015
                     /locus_tag="IVK82_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392921.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19049..19492
                     /locus_tag="IVK82_001860"
     CDS             19049..19492
                     /locus_tag="IVK82_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP6392922.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19509..20594
                     /gene="uxuA"
                     /locus_tag="IVK82_001861"
     CDS             19509..20594
                     /gene="uxuA"
                     /locus_tag="IVK82_001861"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP6392923.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20587..21300
                     /locus_tag="IVK82_001862"
     CDS             20587..21300
                     /locus_tag="IVK82_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6392924.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         7399 a         3302 c         4490 g         6311 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattactt atttctcatt taaacgatgt
     1201 attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt gaataaattt
     1261 cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct agtataacag
     1321 aataagagga acaagtccag aagggatatt tttagtcttt tttaaaataa cttttcagtc
     1381 gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa atagggaagc
     1441 atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa acttttttaa
     1501 attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat aaaaaaaggc
     1561 cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt tccaaaaatc
     1621 acttctcaaa ttttgccaca tccccaattg ggtcaaattt gtttatttga atatattttt
     1681 tacataagga tctttaatag ttacattgtc ttttttcatc actttacgga ttgttttatc
     1741 catgaattcc gtatcaagag tttttgcttc ggtagcaatt ttttctagct cagatttgta
     1801 tttgtcttta ttagaacctt tatcctgttt ttttaccatt tttactagat aatagctttg
     1861 ctgataagtc gttggatctg ttacaggaat gatatcagaa acttccccgt tttttagttt
     1921 aaatgctgct ttttttactt cagtaggaat ctctggattg gttgaatcaa aattcatttt
     1981 tccatcagtt tcctttaatg ttttattttt tccataagct tgaacaagct tggagaaatt
     2041 ttccccttca tttgcttctt tttttgcttt atctgcttct tctttagtcg aaaatagaat
     2101 gatctgtgta ctaacttctg gatagaattc tttccaagca gtgtctagat ctttatctgt
     2161 taattttaca ttggctttca aaccgtattg gaaagccagc atctgtttaa agagtttctt
     2221 atatgtctct tctgtttggc caactgcttt taattgttcc ttgtagtttg gaccgagttt
     2281 tttgatttgt tcattatatt cttttgttac ttcctttgaa gaaatctctt ttccgtatac
     2341 atcatcaaat gctttgaaga caatcatttt ttttaatagt gtttgattat ttgatttaac
     2401 ttttgggtta tcataaaaat cctgaactgt aagtgttcca cctttaaacg ttgcaatcgt
     2461 ttcattggac ttgctacaac ctgagaatag taaaattgca catgctgaaa ggcacataat
     2521 tagtagttgt tttttcaaaa aatttcccat cctttcctta ttaaaaacag tctaacagaa
     2581 gaattaggga gacagtcttt cattttcgta aggttgctgt agatattctt agttcgtaaa
     2641 aatctattct ctctatgatg gagaacattt acatagtttt cacgagacaa gaaagaaaag
     2701 ggttgttaaa cttaatataa aggagggatg aaagatgatc aggattattc ggttgattgg
     2761 atctgtttta atagtcttag ttggttttgt tttaggaatt cttgtcaata atgcacttgc
     2821 ggatatgttt attggaaata gtgaatggat tggagcaatt gccggtacgg tcggacattt
     2881 agctgttttt ttactagctt tatttcttgc tagtaaggtc gaaggaaaaa gaatagaaga
     2941 ttatggtgtt tctgttcgag gaaaagactg ggggtattta gcgggaggtc tcgttgttgg
     3001 aattggagtg tttctgctta ttactagtcc attatatttg acgggggcgt atcggttaga
     3061 ttcaggaaac gcaaacattg tgccgctgat aacgagtttt attttattta ttgcagtggg
     3121 ggcatcagaa gagcttttat tccgtggctt ttttcagcat cagatgctta gatttggtcc
     3181 gttaatagca atgattggat ctgctgcatt atttgctctt ttgcacggat tgaatcctaa
     3241 catgacattt ctggcagttt tcaatatctt tttagcaggt tgctttttca gcgcacttat
     3301 ctacagtaca ggaagtttat ttacggctat tggtgctcat attacttgga attggacaca
     3361 aggagctatt ttgggtatac cagtcagcgg aacgcaagaa caaggttttt tctccactct
     3421 gatccaatcg caaaatacaa taattactgg gggaaacttt ggggctgaag catctatatc
     3481 aactacatta ttcttgttag tcttgattgt tggtttgtta ctctatgcat ataaaacaaa
     3541 gcggatgaag aaatacactg ctgtttatca ataaaagatg aagaaagcaa ggctgatatc
     3601 ggtcttgctt ttttaagtct gtcattgatt aaacgaaaaa gtaaacaaat tcaagcgaat
     3661 acacgacatc ttttgttaga ttttcacaac tgactgtcgt gttctcatca ttttcatttt
     3721 atataattcc tttatcaaat agaaggtaat tatatggaga gtgatagaaa tgaagaaaac
     3781 agcgaccgta ttatttgtta ttcttgggct gagtataatt gcagcaatga ttggaatcag
     3841 tacttctgca aatgaacggg caaatgagaa tctttcattt actgtgaaaa ctgatcggat
     3901 tgtttatgac atgacacaac aagtcattac tattccagtc aaaccaaaca aaagcgtgaa
     3961 tgctagtgat gtacatgctg ttctaacata tggttgggat ggtaatggaa gttcagaaaa
     4021 agttattggg gaagtttatt taaaagacgt acaatggacc gctggtattg agtatacaat
     4081 aatgatttct gcagaactat cgattgatga gatcaagagt aaagataaag ttgatttgat
     4141 tgttttttat gatggacaga tgacgatcac ggagaatttg aaaccaagca gttggacagt
     4201 tgttggaccg ccaagcagta cggatagcac aagtagtgaa agcagtacag aaaacacgag
     4261 cggtgaaagc agtacagaga gtacgagcag tgaaagtagc acagaaaaca cgagcagcga
     4321 aagcagtacg gagagtacga gtagtgaaag tagcacagga agcacgagca gcgaaagcag
     4381 tacggagagt acgagtagtg aaagtagcac aggaagcatg agtagtgaga gtagcacagg
     4441 aagcacgagc agcgaaagca gcacaggaaa agtaaataat gaacttgatt tggtacctat
     4501 cataccaact aaggaacaaa agagaaatgc agggagcaac ctaaaaagtg tgctaaaaca
     4561 gcctgatttt gtttctcgtg gaattgatca tcaagaaaaa gcgaaagaaa cagataatag
     4621 aaaaagcgat ttaccagaaa ctggtagtag aaccttgaac aagctaatta catggatggg
     4681 tgtgttactc attttgattg taggagcaag ctattttcgt tctctcttcc atcgagtgaa
     4741 ataaagaata aaagcaacag atctaaaatt gaataaaacg atagattttt gagtgaatat
     4801 ttcattcgag agtgctgtat tgcctggcga agttgtagct ttgccagatc tatttttgtt
     4861 tactaacata tttgagaaac aggaaataac aggaacacaa atggaaaata agagcaagaa
     4921 taattgacaa tgcacactgc gcatggtagt atctctgaaa aatcagtaac gaatctattt
     4981 gtacgttatt catgtagctg tcggaataag tctaaataat agataaaaag gagtatgtac
     5041 tttgtttgaa gaatttattg atatcaatga aaggcaagta tatcaatttt tgaactattg
     5101 ttatgaaaga gacgagaaat tatatgtggt gaaggatatc gctttagatc taaactacac
     5161 cctggctaag atgaattcgg tgattcaaca agcagaatcg ttttgcgaaa ggtatccaga
     5221 atataaatta tcttttcttt cagaaaacaa gatgataaaa gtcgagtttt cttctcagtt
     5281 tttactgagt aaagtttatt ctatactttt agagggaacg attggttata ttttactgga
     5341 ttcactttac aaaggtactt atcaatcctt agaaaatctt agtcaaaaaa ttatataagt
     5401 ttgcgtacga ttcaaagaaa attaagagaa cttaatacta ttttgaaaaa ctatcattta
     5461 tcttgcagct tgaaaaaagg taatccgata gaaggagaag aataccgtat ccgatacttt
     5521 tttcacttga tgtattggca gtcattcgac gaaaatcatt ctgatatatt atcaagtata
     5581 aaaaataaac gtcaactatt agaaaaattt gcgaaacatg cgccatatac gcgtagtata
     5641 gatagaaaga aatggatcag cctactaaga atttcgttgc aacgaatgaa acaaggccac
     5701 tcgattacgt atgtcccaga agagatgaac aaatttgtgc atccattgat aacaaaagaa
     5761 gagtttacgc attacatact agagcctttt tttgaagcaa acttctggtc gatgaatcac
     5821 atcactccaa cagagcaagc ttacctttat tttatgtttg gtgtaatgaa cacctatttg
     5881 gaagaagatt tgttggatac tccagataaa gtacaagaaa gttggggctg gattgaatcg
     5941 tctattgtag aaaattttga aaaaaacttc aagatttgtt ttacaacttc agaaaaaaat
     6001 tatctatatg tcaatttggc aatgatccat ttgtattcaa gggtttttgg gacaaaaaag
     6061 aaggtggatg cttttggaaa atcagcagaa gacagagatt ttcagcatat atttccagtg
     6121 atgtatccaa tcatgcaaca gttttttagt ggggtagctc gcagaattcc agagcttaat
     6181 acttattatg aaaagaacag acggttgatt tttcaatact gtatgttagt cagggatatt
     6241 tttataaaac acgaacaacc tgtcatattc tatattcaat caaagtatgg taaaacacag
     6301 gaattatggg caaagaaaag gatattatct atgactgaac gcccaataaa atatagtgca
     6361 tccgctgatc agttacctga tattgtcatt agcgattatc ctatcgacaa aaaggcatat
     6421 gatcaaacga atacacagat attttactgg aatggacaac ctacaaaaaa tgattgggcg
     6481 cgtctgaaag ctgaaatcat aaaaatcagg aatgagaaaa atagatccat attggaaaaa
     6541 ttggatttcg ttaattaaga aaagcagatt gtttatgcca aaattaagac attgcaaaaa
     6601 gattattttc tttaaaatcg acaagatgac cagaaagcaa gagttcgttg agctcttgct
     6661 ttcttttagt tttagaaagg aacatcctga ttttatcttc gctaaatcca gtcaaaagtt
     6721 ttgcagattg gtcgtagata atcggttttt tcaataaaga aggggatgta cgaatctcaa
     6781 tatatagttc actcaaactg agttcatcta ttctgtgaac aagattttta taactaatag
     6841 aatgagtaga gagcatttcg gtcacgccgt tgtttgtcaa ttttagtaag tggataaatt
     6901 catttttggt tagtggttga cgaattaagt tctgttcgat aaacggtata tcatggacga
     6961 taagccaacg ttttgcgcgt cttgaagacg agcagccaga acctgtatac aatttaatca
     7021 tcatacattc ctccaatagc taagtattta tgaaataata acatgaagaa atagtcgaaa
     7081 agtgccattt agaggtgtaa aataagaaaa tgttgtcacg tataatggaa ctagctagat
     7141 aagaaatact attaaatcaa gaaaaaagaa agaaggcaca agtatgtcaa acacagccga
     7201 cgcaatagta gtgatcgatt tacaaaaagg ggtatgtttt gattcttcgc cgatacacga
     7261 attagaaaca gtgacagcat tagtgaataa aagaatcaaa acatacgaaa cgattgggaa
     7321 accagttatt ttcgttcaac acgaagatgc tcaattagtg ccacaaacaa aaccatggga
     7381 gctattgtcg gaattatcta ttcctgagca cagttatttt gtgagaaaaa cccatgcgaa
     7441 ttcattttat aagactaatt tgagtgtttt attaactgaa ttggactgtc attcgatcga
     7501 attatgtggg gcacaagcag agtattgtat agatacgact gtgaagtttg ctcatggatt
     7561 aggataccag cttatgatgt gtagaggagc aacgacaact tatgataatc cttttatgaa
     7621 agcaaaggaa acaatcgctt tttacgagaa aatttgggat aaacgatttc tgacattttt
     7681 ggagcaataa gttgtttctt cgtttaaaaa aagcagaatg gatactggtt tatccattct
     7741 gctttattac gtaaaatgag aaaataatcc tgttctattt ttatgggata aaccatttat
     7801 tttttgtata aaaaatgttt gcgtttacat ataattctcc tagaaacata aaaaggagtg
     7861 aatagcttga gaacaaaaca agtattaaag ggagcgttgg tcatcttatt tgctgtaagt
     7921 acaagcgtcc cgcaatggac agtatatgga gagagcattt cttccgaatc ggaatctcgg
     7981 gaggtagaaa ccacagaaac tactgatgct ggaaatgaaa cgaaatcgag tacttcacaa
     8041 acagaaacaa atactgaaac aacggttgag acatctgtta ctagcacaga tcaagaaacg
     8101 accgctacca cagataacca acaaacagaa gaagcaacag ctacaaaaat gtcaaacgac
     8161 gaatcctctt ctgctaaagt aagtgacaaa gagaatggga cagaaaatga tgatatcggt
     8221 aaaggtgaaa atttcaatgg taaagaatat ttgaattact tcggtaaaca gatgacagaa
     8281 atacttaatg gaccaacaga agacatcaag tcaaaattaa tagagttatt agtcactgag
     8341 cttgctgtta atgattatgg tgtagataaa gagattatca aacatctgat tgctacttat
     8401 ctattgtacg atatcgcttt tgcaaaaaac agtgtgttga tgtcgatgct tggaggtaca
     8461 gatgatggga caacgcctac agaaaacaca ggcttctatc caaatacaaa acaacttcat
     8521 caaactgtac taaacaaaca agatggcaaa atgatggatg tatatgcata ttatgtcgat
     8581 caaggttcag ataaaacagt catgatccat ggcggatttc gagggaattg gaataacggg
     8641 atcgttacag aagaatacaa tgatttttat aaggccgggt ataatttatt atttgttgat
     8701 tccagagcga ctggtaatag cggaggcgat tatgtgacct atggacaata tgagtcagat
     8761 gacgtccttt actggataaa tcaagaagtt cgagagcggc catctcaaaa gattttactt
     8821 tatggtggtt cgatgggagc agctacgatg atgtctgtat tagcgaaaga tattcctgtc
     8881 aatgtcaaag gaattattga aaattgcgga tttgcatcaa ttgatgaaca actacgtttt
     8941 acgtacagcc agacagtagc ccccgcacta ccggatgcca taaaaaatca gttagacatc
     9001 attggggatc aagagcatga agatttattt atgggccttt tgaaacaata ctattttgat
     9061 caggagatgc atttggatac aaaagctgct ctacctacga ttggaatgtc agattcattg
     9121 cctaaattga ttatccatgg aacagcagat gatgtagtac ctgtgtctaa cgcgcaaaaa
     9181 ttatatgagt tatcaggagg gtataaagat cttttattag tagaaggagc agggcatgga
     9241 aaggcacagg aagttgacca tgctgcctac acgaaacatg taacggactt tttgaaagta
     9301 gtgttccatg atcagataaa cgtaaagtat gtggatgaaa acaataaaag tctactcaaa
     9361 gaccaggacg aaatccagct ttacggagct tatggagaaa attatatgac agagcaaaag
     9421 acattcgaag gatacgaatt agcaaatgtt gaaggaccga ctgaagggat ttttaatgag
     9481 acgataccaa ctattatttt ctattacaaa aaaatcccag tagctccacc aaaaaagcaa
     9541 gatccgacag agaatgcaga caataaggga aagaatgaac ctcgagagga tcaatcaagt
     9601 gatgatacgc aaccgattca gatgacagtg aaagtacaaa aaaagacaga tgcaaaaaaa
     9661 agaatgttgc cgaaaactgg agaaaagaaa cattcgcttc tcatttattc aggtggattt
     9721 ttatctgtta gcacgattat cacatggatt tggcgaagaa agagagggta aatgaatgaa
     9781 atggttcaag cgaatagtga ttgcagtacc agttatcatc gtagttattt tagtcactgt
     9841 atttttgatt aatcaaatca caccaaagcc gattagcttg cttgttagaa aacaatttga
     9901 cacaagtgaa aaagaacaag tatatgcccg accagatgat ttccagcaaa aaatcgaaca
     9961 aatcgatata aaaaaagaaa tatcctatcc ttcaaagtac aaaaataata atatggatat
    10021 ctacacaccg aaagtggaaa agaaaaaatt accgattctt ttctggatgc atggaggcgc
    10081 atatgtagga ggggataaaa atgattgtcg tgattatttg gaatatctat gttcagacac
    10141 gcagcaaatc atcgtaaata tagattatga acgatcaccg gaagcaaaac atcctacacc
    10201 agttatccaa ttgaacgaag ccattcaagc agctaaaaaa gagataaaag atcaagcaga
    10261 ttggtcgaat atttcgattg gtggaaattc ggcaggtgca caaattagtg gagaatattt
    10321 gttagcactt caaaatgaag aaattaaaaa agctgatcat cttgatccga ctttggagaa
    10381 caagcagatt acgaagttta tttctttgtc tggtctgttg gatccttctg cacttactca
    10441 agtaagtgat aaaatctcta gctttcttta tgaaaaatgt ggatgggctt actttgataa
    10501 taaggacttt gaacaatcag agaaggtcaa aagtttagcc ttggtcagac atgcggatcg
    10561 ttggacgcaa aacacattct taacggatgg aaatacaaat acgtttacga aacaaatgga
    10621 agaagtaact tctgtatttg agaaagcggg ggtgaaagtc acaaaagtag attatgcgga
    10681 gaaaaatgct gttttgaacc atgaatatca gtttgatttc agcaataaac aagcgcaaga
    10741 aacatatcaa aagttagtaa tgtttttcaa ggagtaaagt cttatgatgc aaaagaggtt
    10801 gtaatctgtc acttagctcc aagtcacaag gagcaatttc aataaacaag cttttcatgg
    10861 tttatgaata ttgtaacttg tgactgagga gttagagatg gcaaggggca ccttttattc
    10921 gaaatttggg aatatcacac gattattgtc acgatctctt ttacgtaaat tttataagtc
    10981 agatttgttg ttttgatcgt ttaaaaaaag caacaaatcc gaagcctgcg gaggcaagaa
    11041 gggacaaaaa gccgagtggt aagcaaaaga atgctggttc cactaatatt ccgttgttat
    11101 tgatataagg ctgggaacaa agatgaatag caaataaaat caatgcacta gcgaaaaaaa
    11161 gcaaagaaat atggatagct ttcataagca gcacctcctt ttttataatt gtatgataaa
    11221 tagattaaga atctataaat gtgacaagtt tgttaggatg tgaataaaat gatcatctag
    11281 cgaacttgaa acaaagatgg ctaatgataa gcaattaatg attcagcaat cagataggta
    11341 caaagtttac catcatacgt ctagaccaat aatagaaaat atgggaatat aatagaaaag
    11401 atttaggata cccccaaaag aatttcccca aaaattcgat gtatcctaaa cttttttatt
    11461 ttacataaca atcatatcaa ctgtgaaata aaattacaaa tgataaccta aaatgaggcc
    11521 aaaagccagt gttatcaagg aaatacttaa ttcaccaaaa ttttttttaa ttttttagaa
    11581 aataagaaac aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac
    11641 tttgttatcg catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc
    11701 gatgacatac gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa
    11761 aaagccacta ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc
    11821 tgttatggtg ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac
    11881 gtgattcttt ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt
    11941 gagaaatacg ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa
    12001 aaacgacatc gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt
    12061 ttgctccagt ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt
    12121 gagaaatcaa aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat
    12181 ggtaatccgt gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag
    12241 ttgctacaat ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa
    12301 tagtagccgc tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg
    12361 aaatactaga ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat
    12421 tttcgtggat agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat
    12481 ctttaaatcc attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc
    12541 ccagttcact tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa
    12601 tataatcggc gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta
    12661 gtcttatgga gacgctcatg agaattcctc attttcataa taagattatc attttttccg
    12721 ttctcattaa tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg
    12781 taaaatctat ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta
    12841 tcatggaaat tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa
    12901 aagagcacca tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa
    12961 gagaaaatga cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga
    13021 aaaaaatcat ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac
    13081 tttttccaaa actcggacca ggagacatta ttattgaagg cggaaactct tattataaag
    13141 attcaatccg tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga
    13201 cttctggagg aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag
    13261 aaacttttct atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt
    13321 atgctggtgc atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg
    13381 gaatgatgca agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg
    13441 acttggaaca agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg
    13501 aaatcatgga agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt
    13561 ctgcatcagg tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc
    13621 cagtaattgc aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag
    13681 caaaagtagt ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg
    13741 agtaaaatgc cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt
    13801 ccacaccact tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa
    13861 gagattccag gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt
    13921 gttacgccat tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc
    13981 cgacatggta ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc
    14041 gtatctatca tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat
    14101 ttgttgtcca tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg
    14161 gaagatcggg cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt
    14221 gcaggaaata ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt
    14281 ctagcttatg atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta
    14341 gaacttcaag aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa
    14401 gagaactatc acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta
    14461 tctaatagtg caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc
    14521 ggaaagatcg caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat
    14581 ccgtatttag cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa
    14641 tgtttagaag caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt
    14701 cttcctgaac gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg
    14761 tgaacggttt aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt
    14821 caaaagtttc tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa
    14881 cggtatcatg ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat
    14941 ttttttatca tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa
    15001 tcatcaaaaa aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct
    15061 agtgggttat agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag
    15121 taattgtgct ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg
    15181 gaaaacagaa cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat
    15241 tgattcccct gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga
    15301 atctgagctc atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca
    15361 agatactgcg aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga
    15421 tatggcacaa agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt
    15481 tgctggttta gttggaggat taggagataa atacgcaaga aatgtggcag cacatgctat
    15541 gcatgacgct atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt
    15601 agcgtatggg attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt
    15661 atatcctttg tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc
    15721 gaaagataaa cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca
    15781 tttgattcct atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg
    15841 ttatatcaga aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt
    15901 tgttgattgc tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt
    15961 tgaccttacg tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag
    16021 ttgcacaagc gatgattgtt acagccgctt tgcaaatgat ttatatgggg gttttttctc
    16081 caggaggatc aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat
    16141 tattagggga tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg
    16201 gaagttactt gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca
    16261 aagctgtaga agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg
    16321 ttgcctcatt tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat
    16381 tgatcgctga tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag
    16441 taggtggagg gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag
    16501 attatctggt ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata
    16561 ttacgatggt tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc
    16621 aaaaagggaa acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg
    16681 aagatgacta tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga
    16741 taatcaaaca ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag
    16801 atggcatttt gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact
    16861 atacggaatg atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc
    16921 atataaacgg cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac
    16981 tggcctaatg tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat
    17041 cttgatgcag gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg
    17101 aatcggagat gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc
    17161 ttgggcacaa cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta
    17221 tcaggtttta ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac
    17281 aggcctaaag caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt
    17341 gtttatgatg ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc
    17401 gatatccgga cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt
    17461 acctttagct gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac
    17521 acaagcatta ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata
    17581 aaaagaaggg aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt
    17641 gatccatggg caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt
    17701 tgtagacgaa gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag
    17761 ccgtacagga caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga
    17821 atatcaattt aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga
    17881 cttagtaaaa cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa
    17941 tcctgataca gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa
    18001 attgaaagaa atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc
    18061 tgcacctaaa acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc
    18121 ttagaaaaaa gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga
    18181 atttcgttat cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct
    18241 ttcagcatta gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga
    18301 tacgctagcc taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt
    18361 tatctgtact tattacgata cacccgttca tgcattagga agttatttca tgtttgattg
    18421 gaaagaccaa aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag
    18481 tttaggttgg tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct
    18541 atccgtataa acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa
    18601 agcagctcgc ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac
    18661 gatgctagaa atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga
    18721 aggctgttat ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat
    18781 tcttttttat ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt
    18841 ttcaaataaa gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta
    18901 tatttttagt gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg
    18961 agggaaaaca tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa
    19021 aagtgcaaaa caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa
    19081 gttgagcgaa ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga
    19141 aacaatgtgc ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa
    19201 attaactatg atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag
    19261 tccgtacaat caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac
    19321 ttatgtcata gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct
    19381 tcacacaccg atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac
    19441 tgtttggcat gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg
    19501 ctgaaaagat gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa
    19561 aatatgttcg ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc
    19621 ctggagatgt ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag
    19681 gactggaact gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag
    19741 atgaacgaga ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg
    19801 gggttcgatt gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt
    19861 ttaatgaaaa caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga
    19921 tggagccagg agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg
    19981 gttgggaaga agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga
    20041 ccgaagaaga attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg
    20101 aaaaagcaga tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt
    20161 ttcctagagt gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc
    20221 catataatgg actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag
    20281 ttgaaatcat ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt
    20341 ttttaggaga aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata
    20401 tgtatcatat tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc
    20461 atggtagaac cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa
    20521 tgggactcac ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag
    20581 agcattatga ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact
    20641 ggacgtttag cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt
    20701 aaggaagtct attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc
    20761 attttgtacg gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa
    20821 gaaaaaatcg tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta
    20881 tcttttgcaa aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa
    20941 gtagaaatgt atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg
    21001 gatgatgtaa aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg
    21061 aatattcctc caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca
    21121 aggcgagcgg gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat
    21181 aatgggaagc ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat
    21241 gaattttatg acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa
    21301 taaagaggac tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat
    21361 gaaaaagaag taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa
    21421 caaacacaaa acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga
    21481 atcgtccata tgagggtttc ca
//
LOCUS       DACCCS010000046        21299 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.047, whole genome shotgun sequence.
ACCESSION   DACCCS010000046 DACCCS010000000
VERSION     DACCCS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.047"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            68..808
                     /locus_tag="IVK82_001863"
     CDS             68..808
                     /locus_tag="IVK82_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6392925.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="IVK82_001864"
     CDS             complement(1232..1402)
                     /locus_tag="IVK82_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6392926.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="IVK82_001865"
     CDS             complement(1596..1775)
                     /locus_tag="IVK82_001865"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392927.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2162..3264
                     /locus_tag="IVK82_001866"
                     /pseudo
     CDS             2162..3264
                     /locus_tag="IVK82_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(3342..3635)
                     /locus_tag="IVK82_001867"
     CDS             complement(3342..3635)
                     /locus_tag="IVK82_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP6392928.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3803..4117
                     /locus_tag="IVK82_001868"
     CDS             3803..4117
                     /locus_tag="IVK82_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP6392929.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4138..6117
                     /locus_tag="IVK82_001869"
     CDS             4138..6117
                     /locus_tag="IVK82_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6392930.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6130..6456
                     /locus_tag="IVK82_001870"
     CDS             6130..6456
                     /locus_tag="IVK82_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP6392931.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6472..6990
                     /locus_tag="IVK82_001871"
     CDS             6472..6990
                     /locus_tag="IVK82_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392932.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6990..8342
                     /gene="celB"
                     /locus_tag="IVK82_001872"
     CDS             6990..8342
                     /gene="celB"
                     /locus_tag="IVK82_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP6392933.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8426..9931)
                     /locus_tag="IVK82_001873"
     CDS             complement(8426..9931)
                     /locus_tag="IVK82_001873"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP6392934.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10023..10613)
                     /locus_tag="IVK82_001874"
     CDS             complement(10023..10613)
                     /locus_tag="IVK82_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6392935.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10792..12438
                     /locus_tag="IVK82_001875"
     CDS             10792..12438
                     /locus_tag="IVK82_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6392936.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12487..13206
                     /locus_tag="IVK82_001876"
     CDS             12487..13206
                     /locus_tag="IVK82_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP6392937.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13685..14632
                     /locus_tag="IVK82_001877"
     CDS             13685..14632
                     /locus_tag="IVK82_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6392938.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14644..15564
                     /locus_tag="IVK82_001878"
     CDS             14644..15564
                     /locus_tag="IVK82_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6392939.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15605..17062
                     /locus_tag="IVK82_001879"
     CDS             15605..17062
                     /locus_tag="IVK82_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6392940.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17407..18021
                     /locus_tag="IVK82_001880"
     CDS             17407..18021
                     /locus_tag="IVK82_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6392941.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18014..19738
                     /locus_tag="IVK82_001881"
     CDS             18014..19738
                     /locus_tag="IVK82_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6392942.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19735..21186
                     /locus_tag="IVK82_001882"
     CDS             19735..21186
                     /locus_tag="IVK82_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6392943.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6866 a         3608 c         4314 g         6511 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt gtattcgttt tgttgacttt ggttataaga tttcccattc gatcataggt
     2461 ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat caaatggtca
     2521 gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc aaccgctagt
     2581 gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat tgtttattcg
     2641 ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt attatttttg
     2701 tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa caaagacaaa
     2761 agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat ttggatgctc
     2821 atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc ttttcagcga
     2881 ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt gatgtcgatt
     2941 ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg tgttttattt
     3001 attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg tctagcttta
     3061 ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc tttggcaatc
     3121 actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga gaatatcctt
     3181 ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg aaaaataaga
     3241 gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa aagtgaagat
     3301 acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa atcagtgaaa
     3361 cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg agccatcatt
     3421 tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat cagtaaaaga
     3481 gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc ttctgcacga
     3541 atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc ttgtccattt
     3601 gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga aaaaattcta
     3661 tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca ttttttccgc
     3721 aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct tggtttataa
     3781 aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg caggaatgtc
     3841 atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag aagaggtact
     3901 cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg attttgattt
     3961 atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat taggagaatc
     4021 tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc ctacaggcat
     4081 ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg gatgataatg
     4141 aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga gtatgtcacc
     4201 agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa ttacttgcag
     4261 cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc aggttatggc
     4321 taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg gcaagaaata
     4381 gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg acaaaagtat
     4441 atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt ggcagaagag
     4501 ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga gcgattgatt
     4561 ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga aggggaagaa
     4621 agaaatcgac ggcattttat catggatact ttttttggaa accattatgg aaattcattg
     4681 aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga gctagtcatt
     4741 atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat catacagaat
     4801 ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga aatcaaggaa
     4861 ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca gcggatcgtt
     4921 caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc ttatttgaca
     4981 ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga actatcaaag
     5041 gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt agtagaggat
     5101 ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg attgagccga
     5161 ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc caaagtcttt
     5221 gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac catcaatgaa
     5281 gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga aagagctgcg
     5341 cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca actattaaag
     5401 aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa aggctattat
     5461 gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat tgatctttcg
     5521 acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga agatgatgtt
     5581 cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga tagaccgata
     5641 ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc tgaaaaattt
     5701 ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt gctgcaaaag
     5761 ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat ccagcaacgt
     5821 gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc cgctgttcca
     5881 gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca gtgggatcaa
     5941 tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa tgaaggaatt
     6001 acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca gcaggaaatc
     6061 ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat cgaataagga
     6121 ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc ttttgatatc
     6181 attttccata gcggaaatgc acggacactg atacatgaag catttcgctc gatgcgtaaa
     6241 gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat tcttcatgcg
     6301 catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat agaaatggaa
     6361 attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt ggaagttgca
     6421 caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat tgtgcaggat
     6481 acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa catgtatttc
     6541 aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa catgcaatgg
     6601 atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat tctgatttta
     6661 gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca aacgaacgtt
     6721 ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt gattccagtg
     6781 ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa tggaggacag
     6841 ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat acagtatcgt
     6901 ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca atatgaaaaa
     6961 gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa tacctgatgg
     7021 gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca gcggggatgg
     7081 cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt ttgccattac
     7141 cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt gacttgtaca
     7201 tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt gtgtttgctt
     7261 atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg ctcacggggg
     7321 cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg actgatggta
     7381 aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt attaggatgg
     7441 gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt atgtcgatta
     7501 ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa atgccagatg
     7561 ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt gtcattgcgt
     7621 ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc ttcaaaatga
     7681 ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt gtcatggtga
     7741 tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat atcatcactt
     7801 ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc gcaaattatc
     7861 cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt gcgactttag
     7921 gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg ttaggaaaag
     7981 cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg atgccaatcg
     8041 tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct gctacactcg
     8101 cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg ccgtggcctt
     8161 caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct gctgtgttag
     8221 cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa ttctatgata
     8281 ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa aagttaaaat
     8341 gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt cttagaaatg
     8401 ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg attgcataat
     8461 ccccttcata aggttcatct acaccattga ttttttgata gggatccacg ccttttcccg
     8521 caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc gcttcaggac
     8581 gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg atttcttccg
     8641 cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg atgtcagctg
     8701 tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt cctgggctgc
     8761 ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc agcaatgttt
     8821 tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg gattggatca
     8881 attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca tcttgactgg
     8941 aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg ttgaatcctc
     9001 cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca tgagaagata
     9061 aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt tcttccgaac
     9121 gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt aaatcaaaat
     9181 agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt cgcttgcaat
     9241 agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc gagatattca
     9301 agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa gcttgtgaag
     9361 aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc atacgatata
     9421 aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca tctaacgttg
     9481 tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag attgcttttg
     9541 taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact ttcaatttat
     9601 tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg atatccgtta
     9661 cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat tgaataccct
     9721 gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag aaaaacaaag
     9781 tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct ttttctggta
     9841 catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct tttaatagta
     9901 aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta ttcaatttct
     9961 tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga caaaatccag
    10021 ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat aaacaatgaa
    10081 ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac ataataattc
    10141 aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag ccgaaaaaat
    10201 ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac tgtgtacaat
    10261 gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa atgtgaggag
    10321 atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact tcccgctaca
    10381 gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat cataagattg
    10441 atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa atgtccgatg
    10501 gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa tccaactgtg
    10561 ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt cattgatatc
    10621 tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa aaaacgaaac
    10681 cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc cagtttctgt
    10741 tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc gatggataat
    10801 caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg aggctctgct
    10861 tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat cattccctgg
    10921 tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat gggatacaat
    10981 atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat tgcaaaacaa
    11041 actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt tatccgtgca
    11101 ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat cgcctctcct
    11161 tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg ttctttgagt
    11221 atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca aggcaatcaa
    11281 gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat tttttatatg
    11341 ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac ggcggtcacg
    11401 caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct tttgtatttt
    11461 ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa caaaataaat
    11521 attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt tattattgtg
    11581 ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa gatcatgtct
    11641 gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt tagtgccaat
    11701 ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc tactgggtta
    11761 ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa attgacaagc
    11821 ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt tatgttactt
    11881 gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa tgtactgatc
    11941 caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa catgctgcaa
    12001 ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct agtgaaactc
    12061 cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt ggcgacaatg
    12121 atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt agcccgcttt
    12181 aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt gattatgctt
    12241 ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga tagcaaattc
    12301 cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta tggttattta
    12361 gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg tttacgacat
    12421 aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt gaaatggggg
    12481 aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg caaagaagtc
    12541 aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa aagcgataaa
    12601 gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat tacgtatcaa
    12661 aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac tcaggataat
    12721 catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga tctttttcca
    12781 gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga cggggtgttt
    12841 gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc tgaggttagc
    12901 ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat tgatggcgat
    12961 gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac agaaacttca
    13021 attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat ggtcaaagca
    13081 gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg gctgcctaaa
    13141 gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca agtgagagaa
    13201 aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca tctgaaaagc
    13261 agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta tttccgtaga
    13321 agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact tttgttacag
    13381 tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc ctaatactca
    13441 ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg accctcggta
    13501 ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct ctaatactca
    13561 ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc tatactttgt
    13621 ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg aaaggggttt
    13681 ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc gttataaagc
    13741 acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt acatacctgt
    13801 aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt tcttggctag
    13861 cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt cttcggatca
    13921 agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc ttttgaatct
    13981 gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac gactcgttaa
    14041 agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta tcagttactt
    14101 tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca ctggacatgg
    14161 aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct tggtgttcat
    14221 tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg ttatgggaat
    14281 cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc agcagatcaa
    14341 acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt tagccgtagg
    14401 aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt ccggtgcatt
    14461 atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat ccacaggaga
    14521 ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg tcttgttgat
    14581 gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct aaggaggaaa
    14641 aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa gacggctaat
    14701 tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc atttatattt
    14761 gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta cagtttttgg
    14821 ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca ggataaaatt
    14881 ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat caatagcacg
    14941 gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg tcgttttttt
    15001 accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa ttatcttgtt
    15061 atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc aatggctgtc
    15121 agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc tgattcgatt
    15181 attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca gatcgtttta
    15241 ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg ttactggaat
    15301 gactggttca atgctttact atacatccaa gatgataagc tagttccact acagtatctt
    15361 ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc aggaagccaa
    15421 ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat cgtagtcatc
    15481 tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt caaaggtcta
    15541 acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat taggaggaag
    15601 aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt tgttgggaac
    15661 attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca attcggacac
    15721 tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga tggaagtagc
    15781 aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca ttggctgggg
    15841 cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg atatcgcctt
    15901 tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga ctgaattagc
    15961 gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta aaggaaatct
    16021 ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc aacaagtact
    16081 tacttttaac aaagctttac tagataaata cgatctttca attgatggaa tccaatctta
    16141 cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca cggcagcttt
    16201 tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag ggaatacgtt
    16261 accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat acgataatga
    16321 aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac tcattccaag
    16381 tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc taatgcgtgg
    16441 agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag caaaacaaga
    16501 acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac aaatggcaaa
    16561 ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc tagggctgct
    16621 taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag aagcatggga
    16681 aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc caaatacaca
    16741 tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt ctattacgga
    16801 tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac cgattttagg
    16861 attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta atgtcatgaa
    16921 tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat taccgaaatt
    16981 gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc aaaaacaata
    17041 tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac aaaagacgtg
    17101 tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt attaagctaa
    17161 atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact cgaaactaag
    17221 cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg gtattcgctc
    17281 acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa tcaggtcaag
    17341 gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat gtttggagga
    17401 gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt cattataaaa
    17461 ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg attaggtgcg
    17521 gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa agaaatcaca
    17581 ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct gctatttagt
    17641 ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt tcaaatcaaa
    17701 gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg cctatttgct
    17761 acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc taatctgcta
    17821 aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat aattattggt
    17881 agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt tggtgtcatt
    17941 ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga attagataaa
    18001 cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct tactaaatcg
    18061 tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta ccgttatttt
    18121 tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa gtgatgccgt
    18181 tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca tcaaaaacca
    18241 gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa aacccataga
    18301 tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag tttctttccc
    18361 aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat atatcgtgtt
    18421 gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg aaataaaaaa
    18481 ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac aaggaggagc
    18541 tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga ttttagaaaa
    18601 tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa atcgactata
    18661 cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa tgatcatcac
    18721 agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa acaattttgt
    18781 tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata aaaaaagtgt
    18841 aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta ttttagacaa
    18901 tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag tatctatcgt
    18961 catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg acataacaaa
    19021 cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg atagcatcaa
    19081 tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac atatgcgggc
    19141 gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata tccgaatgta
    19201 tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt ccgctttgct
    19261 tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg acttttgcga
    19321 aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc attttatgat
    19381 cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat tagttgaaaa
    19441 ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg tcaaagtctt
    19501 gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga tgactgaaaa
    19561 acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc acggttctat
    19621 cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt attatatgtc
    19681 gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg agggatgagc
    19741 gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt aaaaaagata
    19801 attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc caaagaagga
    19861 ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac aatgccagaa
    19921 ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt tgaatttatg
    19981 attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt aggcgccgtg
    20041 aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa gaaaatcaaa
    20101 acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca agaactgtta
    20161 ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat aataggacaa
    20221 ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca tgatactctt
    20281 atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa ttacggagac
    20341 cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg ttgttttgtt
    20401 caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac agattctttg
    20461 gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca tcaattttat
    20521 ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga tcaaaccatc
    20581 gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc tcaaaaaatg
    20641 atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga tatacgatat
    20701 tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga agatgaggaa
    20761 tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact tcagcagcta
    20821 cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa tgtggaaaaa
    20881 gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga agtgtcagaa
    20941 aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac gaaaaaaagt
    21001 ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct tttgcacagt
    21061 aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac gtatttctct
    21121 aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa cagtggagaa
    21181 tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta gaaatctaat
    21241 tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc gataaacgg
//
LOCUS       DACCCS010000047        20664 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.048, whole genome shotgun sequence.
ACCESSION   DACCCS010000047 DACCCS010000000
VERSION     DACCCS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.048"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            168..362
                     /locus_tag="IVK82_001883"
     CDS             168..362
                     /locus_tag="IVK82_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392944.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IVK82_001884"
     CDS             631..930
                     /locus_tag="IVK82_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392945.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IVK82_001885"
     CDS             complement(1027..1461)
                     /locus_tag="IVK82_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6392946.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IVK82_001886"
     CDS             complement(1693..2403)
                     /locus_tag="IVK82_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP6392947.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IVK82_001887"
     CDS             complement(2393..3253)
                     /locus_tag="IVK82_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP6392948.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IVK82_001888"
     CDS             complement(3258..3896)
                     /locus_tag="IVK82_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP6392949.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IVK82_001889"
     CDS             complement(3911..4210)
                     /locus_tag="IVK82_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6392950.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IVK82_001890"
     CDS             complement(4253..5737)
                     /locus_tag="IVK82_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP6392951.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IVK82_001891"
     CDS             complement(5757..6218)
                     /locus_tag="IVK82_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6392952.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IVK82_001892"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IVK82_001892"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP6392953.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IVK82_001893"
     CDS             7521..8291
                     /locus_tag="IVK82_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6392954.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IVK82_001894"
     CDS             complement(8564..9391)
                     /locus_tag="IVK82_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6392955.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IVK82_001895"
     CDS             9959..11194
                     /locus_tag="IVK82_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP6392956.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IVK82_001896"
     CDS             complement(12193..12453)
                     /locus_tag="IVK82_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP6392957.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IVK82_001897"
     CDS             complement(12480..13781)
                     /locus_tag="IVK82_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6392958.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="IVK82_001898"
     CDS             complement(14073..14531)
                     /locus_tag="IVK82_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6392959.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15869)
                     /locus_tag="IVK82_001899"
     CDS             complement(14544..15869)
                     /locus_tag="IVK82_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP6392960.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17298)
                     /locus_tag="IVK82_001900"
     CDS             complement(15886..17298)
                     /locus_tag="IVK82_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6392961.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="IVK82_001901"
     CDS             complement(17315..18079)
                     /locus_tag="IVK82_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP6392962.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="IVK82_001902"
     CDS             complement(18183..19058)
                     /locus_tag="IVK82_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6392963.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="IVK82_001903"
     tRNA            complement(19313..19397)
                     /locus_tag="IVK82_001903"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="IVK82_001904"
     tRNA            complement(19415..19486)
                     /locus_tag="IVK82_001904"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
     gene            complement(19496..19568)
                     /locus_tag="IVK82_001905"
     tRNA            complement(19496..19568)
                     /locus_tag="IVK82_001905"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19533..19535),aa:His,seq:gtg)
     gene            complement(19630..19714)
                     /locus_tag="IVK82_001906"
     tRNA            complement(19630..19714)
                     /locus_tag="IVK82_001906"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19678..19680),aa:Leu,seq:caa)
     gene            complement(19735..19805)
                     /locus_tag="IVK82_001907"
     tRNA            complement(19735..19805)
                     /locus_tag="IVK82_001907"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Cys,seq:gca)
     gene            complement(19851..19922)
                     /locus_tag="IVK82_001908"
     tRNA            complement(19851..19922)
                     /locus_tag="IVK82_001908"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19888..19890),aa:Gln,seq:ttg)
     gene            complement(19934..20006)
                     /locus_tag="IVK82_001909"
     tRNA            complement(19934..20006)
                     /locus_tag="IVK82_001909"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19971..19973),aa:His,seq:gtg)
     gene            complement(20009..20082)
                     /locus_tag="IVK82_001910"
     tRNA            complement(20009..20082)
                     /locus_tag="IVK82_001910"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20048..20050),aa:Trp,seq:cca)
     gene            complement(20089..20169)
                     /locus_tag="IVK82_001911"
     tRNA            complement(20089..20169)
                     /locus_tag="IVK82_001911"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20133..20135),aa:Tyr,seq:gta)
     gene            complement(20171..20246)
                     /locus_tag="IVK82_001912"
     tRNA            complement(20171..20246)
                     /locus_tag="IVK82_001912"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20211..20213),aa:Phe,seq:gaa)
     gene            complement(20282..20354)
                     /locus_tag="IVK82_001913"
     tRNA            complement(20282..20354)
                     /locus_tag="IVK82_001913"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20319..20321),aa:Asp,seq:gtc)
     gene            complement(20364..20436)
                     /locus_tag="IVK82_001914"
     tRNA            complement(20364..20436)
                     /locus_tag="IVK82_001914"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20401..20403),aa:Val,seq:tac)
     gene            complement(20452..20523)
                     /locus_tag="IVK82_001915"
     tRNA            complement(20452..20523)
                     /locus_tag="IVK82_001915"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20488..20490),aa:Glu,seq:ttc)
     gene            complement(20538..20627)
                     /locus_tag="IVK82_001916"
     tRNA            complement(20538..20627)
                     /locus_tag="IVK82_001916"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20591..20593),aa:Ser,seq:gga)
BASE COUNT         6531 a         4319 c         3275 g         6539 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
    19501 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    19561 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
    19621 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
    19681 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
    19741 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
    19801 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
    19861 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
    19921 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
    19981 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
    20041 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
    20101 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
    20161 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
    20221 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
    20281 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
    20341 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
    20401 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
    20461 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
    20521 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
    20581 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
    20641 ctcaatactc tttctcaaaa tgag
//
LOCUS       DACCCS010000048        20258 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.049, whole genome shotgun sequence.
ACCESSION   DACCCS010000048 DACCCS010000000
VERSION     DACCCS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.049"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            291..1430
                     /locus_tag="IVK82_001917"
     CDS             291..1430
                     /locus_tag="IVK82_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP6392964.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="IVK82_001918"
     CDS             complement(1497..2072)
                     /locus_tag="IVK82_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6392965.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="IVK82_001919"
     CDS             complement(2077..3051)
                     /locus_tag="IVK82_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP6392966.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="IVK82_001920"
     CDS             3334..4302
                     /locus_tag="IVK82_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6392967.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="IVK82_001921"
     CDS             4415..4693
                     /locus_tag="IVK82_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP6392968.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="IVK82_001922"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="IVK82_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP6392969.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5159..5905
                     /locus_tag="IVK82_001923"
     CDS             5159..5905
                     /locus_tag="IVK82_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6392970.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="IVK82_001924"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="IVK82_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP6392971.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="IVK82_001925"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="IVK82_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP6392972.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="IVK82_001926"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="IVK82_001926"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP6392973.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="IVK82_001927"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="IVK82_001927"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP6392974.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="IVK82_001928"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="IVK82_001928"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP6392975.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="IVK82_001929"
     CDS             10642..11184
                     /locus_tag="IVK82_001929"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP6392976.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /locus_tag="IVK82_001930"
     CDS             11207..12763
                     /locus_tag="IVK82_001930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP6392977.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="IVK82_001931"
     CDS             12777..13679
                     /locus_tag="IVK82_001931"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP6392978.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="IVK82_001932"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="IVK82_001932"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP6392979.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="IVK82_001933"
     CDS             15122..15544
                     /locus_tag="IVK82_001933"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP6392980.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="IVK82_001934"
     CDS             16013..16243
                     /locus_tag="IVK82_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP6392981.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="IVK82_001935"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="IVK82_001935"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP6392982.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="IVK82_001936"
     CDS             17654..17830
                     /locus_tag="IVK82_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP6392983.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="IVK82_001937"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="IVK82_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP6392984.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="IVK82_001938"
     CDS             18325..18708
                     /locus_tag="IVK82_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6392985.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="IVK82_001939"
     CDS             18877..19761
                     /locus_tag="IVK82_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6392986.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19878..20111
                     /locus_tag="IVK82_001940"
     CDS             19878..20111
                     /locus_tag="IVK82_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP6392987.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT         6737 a         3537 c         4402 g         5582 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtg
//
LOCUS       DACCCS010000049        19482 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.050, whole genome shotgun sequence.
ACCESSION   DACCCS010000049 DACCCS010000000
VERSION     DACCCS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.050"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            99..746
                     /locus_tag="IVK82_001941"
     CDS             99..746
                     /locus_tag="IVK82_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP6392988.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IVK82_001942"
     CDS             1139..3784
                     /locus_tag="IVK82_001942"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP6392989.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IVK82_001943"
     CDS             4094..5407
                     /locus_tag="IVK82_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP6392990.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IVK82_001944"
     CDS             5397..6050
                     /locus_tag="IVK82_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6392991.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6776
                     /gene="radC"
                     /locus_tag="IVK82_001945"
     CDS             6105..6776
                     /gene="radC"
                     /locus_tag="IVK82_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAP6392992.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6671..8023)
                     /locus_tag="IVK82_001946"
     CDS             complement(6671..8023)
                     /locus_tag="IVK82_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6392993.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(8089..9378)
                     /locus_tag="IVK82_001947"
     CDS             complement(8089..9378)
                     /locus_tag="IVK82_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP6392994.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(9465..10169)
                     /locus_tag="IVK82_001948"
     CDS             complement(9465..10169)
                     /locus_tag="IVK82_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAP6392995.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            10369..10635
                     /locus_tag="IVK82_001949"
     CDS             10369..10635
                     /locus_tag="IVK82_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6392996.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(10803..11033)
                     /locus_tag="IVK82_001950"
     CDS             complement(10803..11033)
                     /locus_tag="IVK82_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP6392997.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            11105..11260
                     /locus_tag="IVK82_001951"
     CDS             11105..11260
                     /locus_tag="IVK82_001951"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6392998.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(11569..11793)
                     /locus_tag="IVK82_001952"
     CDS             complement(11569..11793)
                     /locus_tag="IVK82_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP6392999.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            12060..12395
                     /locus_tag="IVK82_001953"
     CDS             12060..12395
                     /locus_tag="IVK82_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393000.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            12628..13569
                     /locus_tag="IVK82_001954"
     CDS             12628..13569
                     /locus_tag="IVK82_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP6393001.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            13571..14461
                     /locus_tag="IVK82_001955"
     CDS             13571..14461
                     /locus_tag="IVK82_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP6393002.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            14504..15304
                     /locus_tag="IVK82_001956"
     CDS             14504..15304
                     /locus_tag="IVK82_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP6393003.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            15319..16170
                     /locus_tag="IVK82_001957"
     CDS             15319..16170
                     /locus_tag="IVK82_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6393004.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            16167..16526
                     /locus_tag="IVK82_001958"
     CDS             16167..16526
                     /locus_tag="IVK82_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP6393005.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            16541..16702
                     /locus_tag="IVK82_001959"
     CDS             16541..16702
                     /locus_tag="IVK82_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP6393006.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            16699..17025
                     /locus_tag="IVK82_001960"
     CDS             16699..17025
                     /locus_tag="IVK82_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393007.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            17022..17837
                     /locus_tag="IVK82_001961"
     CDS             17022..17837
                     /locus_tag="IVK82_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAP6393008.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            17976..18287
                     /locus_tag="IVK82_001962"
     CDS             17976..18287
                     /locus_tag="IVK82_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393009.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            18284..18514
                     /locus_tag="IVK82_001963"
     CDS             18284..18514
                     /locus_tag="IVK82_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393010.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            18521..18724
                     /locus_tag="IVK82_001964"
     CDS             18521..18724
                     /locus_tag="IVK82_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP6393011.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            18721..19020
                     /locus_tag="IVK82_001965"
     CDS             18721..19020
                     /locus_tag="IVK82_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393012.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVINNEV"
     gene            19263..>19482
                     /locus_tag="IVK82_001966"
     CDS             19263..>19482
                     /locus_tag="IVK82_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP6393013.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAIL"
BASE COUNT         6581 a         3218 c         3986 g         5697 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttccggaata tataagttag
     6661 tacacataaa ttatataagg aaatttactg ttatattgcc atctggaaat actattatgt
     6721 tatcgataat tgaacgccat aatgcatttt tttcttcttc cgaagattct ttataaattt
     6781 catcgaattc ttttgataga ataaattcga cattttttat atctatttta gctggctcta
     6841 cttcaatttc atttaattct ttcattaaag cttctcgatc ttttttgtat tcctctaagt
     6901 ctataacttc attcaagtag gctttcttca atctatctaa tttattctca atatttttct
     6961 tactattttt ctttttaata atctttggtt gtttttttga atagtttatt tgtatttgtt
     7021 caaatttttg tttagtagct tcaaaaatat aacgttctaa atttttttcg taaaaagcgc
     7081 gcttatttgt gcaacgtaat ttattatttc ttcgttttac acatctataa caagtccttt
     7141 cgtatataga ttctgtaccg tcttttttct tatatcttgc tttaatagtt tgaccagcta
     7201 ctgatccctg acagtcagaa cattttaata aacctttaaa aatgtaatca cgttttgcat
     7261 ttgtttttaa atttttaggt aaggcttttt gaacagcata aaaaagtttt ttatcaataa
     7321 taggaggaca gtaattttta ttatttctta attcaccaat gtattttttg ttcgtcaaca
     7381 tatttttaac actttgataa tctctaacga ttccatactg agagcctaaa taataaacag
     7441 tttttcttaa actaccacta gaaagaaagt agttgaatat atctatcacg ataggagcat
     7501 catcatttat tactaatttc ttattttcta tagagtagcc taacggagtt ttcccgctta
     7561 ctacttcacc ttgtgcaatt ttatttgcca taacagcttt gatgcgttct gaatcaattt
     7621 gggcttccaa ttcagcaaaa ctcattactt gtgcaatgaa agttctgcca tacgctgtag
     7681 ttgtatcata atattgttga gaaacagcat tccaagaaac attgtgtttt tctaaaattt
     7741 cttgagtgtt taaataatgt cgtagatttc taaaccatct atctaatttt gtaaataaaa
     7801 ttagatctat tttattttct tttacatctt gtaatagttt ttgaaattca tcacgattta
     7861 ttttttgacc agaaatacca ccgtctacat attcattaac tactttcaaa tccttgtgtt
     7921 gttcgatata ttcatagagg gtttcttttt gttctctaag actatctcca tgtttggctt
     7981 gttgttcagt tgatactctc atatataatg ctactcgttt catgttatct cctttcgaat
     8041 gtatgttctt atcgttgtaa aagaaaagcc cgaaggctaa tctttttact acacatctgt
     8101 taattctaat gatcttctgt attgttcagc ttgagctagt ttagtaaaag atacagttcc
     8161 gttataattt tctaaaacaa ctttttcaat ttcatcaaga gatactttaa agaattcttt
     8221 tcgttgatta actttattga cttcgttttt tctaaaatgc gaatgtagtt gattttctaa
     8281 tgttggtgca tcttctgaaa atatcattgc atgaacatca aattcaaaag gtacagaagc
     8341 gctactaagt tccttaattc tatccatagg ttctaaccgt ctagtcattc ctattttata
     8401 gacatcttca ccgaaagatc cgatattact gatgacataa acatagccag ctctagtatt
     8461 aagttctctt tgcaatacat cttctttgac agtttctagt tctcttaatt tctcttccag
     8521 ttctttaatt ttatcagcat agagttcttt ttcaacatca gcactagctt tttgcatgta
     8581 tgccattaac ttagttatct ctgagttaaa ctgtcgttct tctttatcaa tgcgtttctt
     8641 ttcacgttct atttcacgtc ttaccttttc ttcttcaatc atttgttctt ttattgcttt
     8701 ttgctgttct ttttcatcgt taactttaac tatgtatgaa tagttgagtt ctaattcttg
     8761 aagttttaat tcaagtaatt cttttttgaa ttctgcttca tcgtttttaa aaagcttgtt
     8821 tattttatca aaagttctta taattttttc tctagttgaa tctacgttag aaatagtaag
     8881 tttattgaga agtccagtta tttctgattc taatggtata agcattttct ttttaatatt
     8941 attagcttct ctattattca aataagaagg aatattaatt gcaccgtcta aaataaaatc
     9001 cttttcttta atctttaata aagaaagttt gttttttatt tcggatgaag aagtttcatc
     9061 tatattcgga ataattactg atttatagat agtgaccaga gatgtgtcat atttttgatt
     9121 aatttcatcg tctaaccgct ggctttcagc attaagaata tcaattttgt tttctagcgt
     9181 tgacaagttt ttattcaatt tacttatgtt tttctctttt tcagatataa acgtttccct
     9241 agcatttaat tcttgagaat attcctttac cttactctct aaggcagaag ttttatcttt
     9301 taatttttta gcttcagata atttgctact acccataatt attaaaacta ttccaactaa
     9361 aaacaatagt actcccattc aaaacgcctc gcttctttta taaaataatt gtcaaaatca
     9421 tataaaccct taccgaagtt gacggctccg gcatgggaat ttttttaaaa tacaactttt
     9481 ccgataattc taatctgttc tggcgataca attctatctt cgtataagtc gtttatagaa
     9541 cgtaaaatga ttgtttgatt ttcgtaatct ttatatatct ctttacatgt aactccgtca
     9601 ccacctaatt ccacaatagc aatttcccca tcttctacgt ccggttgttc tttaacgaat
     9661 acgaattgtc cattcctgat aacaggttcc attgaatcgc ctataatagg tactgcgaag
     9721 tcagcagcac ttggaactct ctcaaattct tcctcttcga caaccgtatc accgtaagta
     9781 agttctgttg gattggcagc cgaagccgaa ttgtgaacag ttgggatttt ttctttttta
     9841 ggaaaatcta ggacattttc ttgtttttgt tcttctaatc gattacttgc aaattcatag
     9901 gtagcaactt tattttcgtc gttaagctga ttatatatag gaaggatatt atatatctga
     9961 ttagatatag ataaatcaat attcatcaaa tcatctatat caacattgaa tatcttagct
    10021 atctcattta aggtttttaa ttttggtgta tattttcctt tttcccattc gcttattgag
    10081 gatgaacttt ttcttcctag tttttcggct agttcttgtt gatcaatatt atacttttct
    10141 ctaagaaact ttagattttc agcgaacata gtaacacctc cttgagttaa ttgtaacacc
    10201 gtttcggaaa aaatgtaaca ggtttcttag aaaatatttt agtgttttta aagaagtatt
    10261 tacgcaaaat agaaatcgat ttcaggaaaa ctgaaatatg cacttgactt cggaaaaacc
    10321 gaagtatata atttagtcag aaagttaaag agaggaggcg aataaaatat ggataccgcg
    10381 aagaaagaaa aatatacatt gagacaatta agagctttaa agggttattc aaaagaagaa
    10441 ctgtcaagga aaagcaaagt gacatcaaga acaatattta tttacgaaaa cgatgtagat
    10501 aaaatgagga acggaaagta cgccactcta gaaaaaattg ctaaggcatt gggtgttaga
    10561 gtttccgata tttttttaga ccctgattcg gaaaaaccga aatcaataac aggtgaaaca
    10621 aaacaagtca gctaggaggt gtgaatagtg gaagagaaga atataaaagt accgactctt
    10681 gaagaatata caaaaattgt tgtcgaaaca gacgagcaga acccgaaaac aatcgcagtt
    10741 attacggcga atgatattga atcatgcgaa ggttttcgag ttcgaatgac ccctagatat
    10801 gattaatgtt ctttatcacg tggaggtata ggatcgtttc caaaactatc ttttgattgt
    10861 atttgtccat tctttttttg gacaataaac tctgattgct gttttttaga aatcttttta
    10921 ccatactcaa cagcatctga tttattatca aatagttttg tatcttcgga attaccttca
    10981 ccacgtacag cccatttgcc atttctagga gatacccatt gatttttacc cataacaatt
    11041 cacctcgctt tcaagttaat tctattaaga ggtgaaaaga aaacaaactg atggaggtga
    11101 gattgtgagt aacaagccaa aaatcacttt gaatggcaca cctaatcttc aaagattagc
    11161 agatgaaatg gctagaatac gttctagaca ttggggagtt actgtggtgg caaccataat
    11221 tgaacaaaaa gaaaagccca cacagccggc aagcaagtag tgggcaccat agaacaataa
    11281 tattatatca cacactcgcc acttccgaaa agaggagttg ctgctctcct ttcaagtttg
    11341 ccaaagtcca cgtttcttca ttttggcaac tcctcttctt ggaggtggtg acggcgagag
    11401 acgaaggagg gatgacatga aggaagaatc aaaaataggg attttgaatg agaacaatta
    11461 cacaagatta attattgaaa cagaagaagg ggaaaaagta gcagaagtta cattgaccga
    11521 tgcaactcct gctgacggtt atcgtattcg attaactcct aaatatgatt aaccttttgg
    11581 tgggtgtgga tcgtttccgt gactatcacg gctttgaatt tttccatctc tgccatgaat
    11641 aaatagctca gatccttgat ttttagaaat ctctctagcg atttttactg cttctgattt
    11701 tgtattagta tgtgcagtcg ctttagaatt tccagcacct ttgacattcc aaccaccatt
    11761 ggaattaggt acaacatgtt gattttttcc catagttttc acctccattt ctatagaaat
    11821 attttatcaa atagaaatgg agaaattcaa taaacgaaag gagggatcaa atgacattac
    11881 aagagcatca acacttgatg cgaaaactaa atcaggaata ccacgaggaa atgaaaccta
    11941 gattgctagg ggaacaatgg cgtgaacgcc agaagaagtg gttagaaata aaaaagtgac
    12001 tcagccgtcc aaagcaatga gtcacaaacc aaaaattata cctaaggaga ttttatcaca
    12061 tgaataagaa aattgaaaat cttatcgaag aattgaaacg tgaatgccaa aagcaaggtg
    12121 tttctattat ttgtacagcc caaaaagaag gggaacttaa aagtctcgtt tatggtgaga
    12181 caacagaaat tttactttgt cttgctatgc aagaagaaca tttagatgaa aatctcccat
    12241 tgtcagcaca tattatgcgt agaattgctg tggatgctta cgaacaagcg aaaaatgaag
    12301 aagaaaatca accttcaaat catacttttg ttattaataa caaggaagat ttagctgatg
    12361 tgatgactcg tattctcaag ggggaatttc aatgatgaat gaaaaaacaa ttgatctact
    12421 cattatttta tttttcacgt tagtgattgt agcgaaaagt tatgtaatct ttggcttgtt
    12481 attcgcaatt accatgcttg tgctttgcgt gttagcaagc agaaaagagg ttaaacatga
    12541 acgagttagc aaatcttgac aactatttaa ctgatcctga ttatgctaaa ccaccatatg
    12601 aagcgccaat tgatgaggag gatgaagatg agtaaatcta ccttagaaat gagccatcaa
    12661 gaatggctcg aagaccgcaa aagaggtatt ggtggctcag atgttgcaac cgtacttgga
    12721 ttaaacaaat acaaatctcc ttatcaattg tggcttgaga aaacgggtca aattgaattg
    12781 aaagatttag aaagcgaacc agcttattgg ggtaatgttt tagaagaagt ggttgctaaa
    12841 gagtttcagg aacgtacagg caaaaaagta cgcagaagaa accaagtatt tgaacatcca
    12901 ttacatccat ttttaagagc aaatattgat cgggaagtag tgggagaaaa tgccattctg
    12961 gaatgtaaaa cagccaatca atttctcgga aaagagtggg aaggtgaaga ggtaccgctt
    13021 agctatctct gtcaagttca gcattacatg aacgttttaa acaaagacta ttgttatatc
    13081 gctgttttga tcggtggaca aaaattcatt tggaagcgga ttgaacgaga tcaagagctg
    13141 atcgatacaa tcactgaaca attagtagag ttttgggaaa cgaacgttct tggaggtatc
    13201 gagcctgtaa ttgacggaag tggagcgact gctgacttct taaaagaaaa atatgcagat
    13261 gtagaagaaa atcaaacagc tctaccatca cgttttgatg aacttatcga gcaaaaaaga
    13321 gaactcaagc ggacgaaaaa agaaattgaa tcatctattc gtcaagtaga caacgagatc
    13381 atcagtgagc taggtaaacg tgaagccagt atcggtatca cacaaagaaa cattatcagc
    13441 tggaaacttg ttcgtacaag acggatgaac acgaagaaac tagcagagaa atatccagat
    13501 gtcgcaaatg atgaagagat ttataacgtt actgaatcaa gaaggctaac cgaaaaggag
    13561 atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa ttggcagaaa agccacagaa
    13621 acaagttgca ccaggacagt tagggcttaa agctctaatg aatacaccaa caatgagaaa
    13681 gaaatttgaa gaagtacttc atgacaatgc taatgctttt atgtcgaatg ttatgactct
    13741 tgtatctaat gacagttatc ttgcagatag tgaaccgatg tctatcatga gtggtgcgtt
    13801 aactgctgca acattaaatc ttgggctaga taagaattta ggttatgcat atttagttcc
    13861 attcaatagt aaaaacaagc aaacaggaaa atgggaaaag aaagctcaat tcatgcttgg
    13921 ctataaagga tatatccaat tagcccaacg atcaggtaaa tacaaagcat taaatgtgat
    13981 cgaagtttac gaaggagaac taaaaagctg gaaccgactg acagaagagt ttgagtttga
    14041 tccaaatggt agaacgtctg atgaagtcat tggatatgtt ggctatttcg agttactgaa
    14101 tggattcaaa aaaactgtct attggaccaa acaagaaatc gaagctcatc ggattgctaa
    14161 caataaagat cgcgataaga caaagttaag tggtgtgtgg gcatctgatt acaatgcaat
    14221 ggcacgaaaa actgttttga gaaatcttct ttctaaatgg ggaatcttgt ccatcgaaat
    14281 gcaagaagcc accacatcgg atgagagagt ccaaagggtt caagaagacg gcagcattat
    14341 tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa gaagcagagg ttatatctga
    14401 agaaaatgaa gatgtacaaa ctggattatt tgatgcatct aatccgccgt taaacaaata
    14461 atgagggagc tttctccctt gcttttctag aggagaaata cgaatgaaca caggatatgt
    14521 caaattacat cgaaaagtga tgaattcatt cgtttggacc aatccttata tgtataaatt
    14581 atggagcttg tgtttgatga aagcgagtca tgagaatcgc aagatacttt ttaatggaaa
    14641 agagatacaa gtgaacagcg gagaattcgt cacagggcgc gatgctatca catctgagat
    14701 gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt tctgtatgga gatggctaaa
    14761 acaatttgaa aaaaacggaa tgttgaacat caaatcaact acgaaataca gcgttatatc
    14821 aataaaaaac tggtctttgt accaaagcag tgaacaacaa atgaacatca aacgcacaac
    14881 aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat tataagaatg aaaagaataa
    14941 taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac gacaccgact cagtttacta
    15001 cattctcgcg gaggagttat tcaaacagat ttgtcagaat caggaaatca aaaagccgaa
    15061 tctgcaaagt tgggcagata acattcggaa aatgatcgag atcgacaagc ggaccgaaaa
    15121 ccaggtacgg ggaatgattg aatggagtca gcacaatgtg ttttgggcat cgaatatttt
    15181 atctgctaaa aaattgcgag aaaaatacga cacaatggca gcgcaagcaa atcgtgatta
    15241 taaaacaaaa caaactaaaa cgcttgaata cgagaaattt agcacagatg agttgcctat
    15301 ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca tggaaaagct gatacagaaa
    15361 gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc ctttgtatgg atggcgtacg
    15421 aagaaccctg atggctcaga acgttgtaag ccaacctgta tgcaatgtgg gtataaagca
    15481 ttgcgagttc aggaagactt acaaacagaa cgaatttaca acgagagcct gaaagcaaga
    15541 gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc aagcgttgtt tgattgcaca
    15601 ttgcagaatt atcaaattgt cgatcaagaa acaagacaag cggttgaagt aaccaaacgc
    15661 tttgttaatt cagtcttgtt aggaaatcca agtcaccttg ttttaactgg aaaacaagga
    15721 acaggtaaaa gtcacctagc gatggcagcg gcttgggaag tcttgaagcg atcaaactac
    15781 gataaaaaaa tcttatttat cgggttacag gaaatgctgg atcaaatcaa attttcgtac
    15841 aacaatcctg aactcagaaa aacgattgag ggatcgttga ttgcagatat caaaacagcg
    15901 gatttagtca tcattgatga tatcggttca gaactaggaa aagatgcatc agatagtcga
    15961 gcgtttggca taaacacgct aaattcgttc ttggatgcac gacagaacct agcaacgatt
    16021 atcacaacga acttgcttgg tgaagaactg aaaaaagctt acggtacgag aacaatatca
    16081 agaatgtttg tcaactctga tggatttacg atggtatttt ctcaaacagc agacaagcgc
    16141 ataaaaccag tgaaaggtag tatcgcatga ataaataccg taataaaaaa actgttcatc
    16201 gaggtatcaa gtttgattct atcgcggaag cagagtatta cgatctagcc ttgtggcaag
    16261 ctgaagcgaa tggttggaaa gtgaagctcc aggaaaggtt tgagctgatg ccgaagtttg
    16321 aactagaggg caagaagtat cgcaagatcg agtatattcc cgacttcaca ttttacaaaa
    16381 gcggcaagct ggtcaaagtc gtagatgtta aggggatgca gacaaaagac tttaagatca
    16441 aggcaaagtt gttctgtcat caatatcaag tgccgttgat attagccaaa aaatatcgga
    16501 atacgttcaa ggaagagcgt ttttaacgag gtggtccatc atgacaacag aagaagtgat
    16561 tcaaatgcga attcgaagcc ttcggcgtga gattgacgat ctggaacgaa caaaggcagt
    16621 gatggtcaat gaaacggcta gaaaggcaat cgatttgcac atagagaatt taagaaggga
    16681 aatccgtaga ttggaggaat gagcgtggat aaggaagcgg cttggcgaaa attaatgttg
    16741 ctgattcaag atgagaactg gcaagaagat gaagcagtgg ttgctgaagt tcagcgtcta
    16801 ggaaagattg ctaacggacg tatacgaaaa aagccagaca aaagaaaaca gcgcaaaggg
    16861 aaaatcgtcg ttgttttaca cgaaggcaaa attttgatgc aaggaacagc tagtgagctg
    16921 tctgcagaaa ctggatatac gcgtgggact attcgaacgt acgcttggcg aaatcacacc
    16981 gatcgaaaag ggcacgaata taagtatttg gaggaagaaa aatgaacgaa aacaaattaa
    17041 tcaaattggg tgtagcagga gcagtaatcg taggtattgg agttatcgga ggatttaagt
    17101 tcttcgaaaa aatcgataat ggatatgtgg gtgtgcgcta ttcaatgaac ggcggtatca
    17161 aagatgaagc actgacgcaa ggtgtgaaat ttgtagggat tgacaaagtg atccaatatc
    17221 caattcgctt gcaaactatc caatcaaaaa atatttcagt atctacaagc gacggcaaaa
    17281 agacaacgat tgatatcaaa tatgactaca aagttgattc aactaaagca gcaaaaatgt
    17341 acaaggaatt tgggaatatc acttcggaag atatcgaaag tggatggtta aaatctaagc
    17401 ttcaaaaggt cgctcgtgaa gtttatgcga aatatagtct gcttgatgtc ctttcaggag
    17461 attcctctaa agttgaagct gaggtattaa cgaactttgc taaatcagtt gaatctaaag
    17521 ggtttgaagt cgaagacgta acacttggtg ttccagatgt cgataaagaa acacaaaaat
    17581 caatcgatgc gatcattcga gctggtcaag aaaatgaaaa agcgaagcta gacgcagaaa
    17641 ctgcaaaaac tcaagctgat agtgaagctt acaagaaaac aaaagctgca gaagcagagg
    17701 cagaatctaa tcgcaaagtc gccgaatcag taacagacaa tttgattcgt tatgaagaag
    17761 ctcaagctcg caaaaagcat ggatgggtaa cagtaaatgg agcagatact gtagttacgg
    17821 atgaagcagg caaataatat gggattcttt atggctaaaa ttctcttgtt cttaggttta
    17881 gtaggagcag catatctcgt gtatgcgctc ctttcccaaa ctgatgacaa agaagatgac
    17941 aacaacgatg aaatgaaatt ttaggaggag aaataatgga cgaactaatc acaaaagtag
    18001 agcagtgggc taaagacaag ggactggatc aagcagatcc aaaagcacag tttttgaaag
    18061 tagctgagga attcggggaa atagcttcgg cgatggcaag aagtaatgat gaactattta
    18121 aagatagcgt aggagacgtt atcgtcacgc tgattatcct ttccatgcaa aaagggacaa
    18181 acgtacaaga gtgtttagaa atggcataca acgaaatcaa aggacgcaca gggaaaatgg
    18241 tagatggtgt attcgtgaag tcgagtgatt tggaggatgt gaaatgaaat acgaaatacc
    18301 actaagtgaa gcgggcattc aagcaattat caatggtcgg gaggttaaca tagaacttcc
    18361 tgatggtact gaattagtca tcagacaaag ttatttgaaa gatatggcag ctccagtatt
    18421 aattgatcgt tttaacgtga ctgattctgt ggtagagaac cacttaaaag aatttcgatc
    18481 aagtatagac aacactttca gattagggag ttgattgaca atgaacacca gacaccgcag
    18541 agtagcaaaa ctaagaaaac aggaactaaa tgtactaaag acaaagtttg aaaaagaata
    18601 tggaatttca gcagaagaaa catataaagt ggcaagtcag tttgttgctg atgcaagtga
    18661 tgctattcgt aagtttggga tttcgatatt aaatgatgat cgtaaatggg aggaaatgag
    18721 atgaaactaa aagacggatt ttacgctagt agtcacggca tcggcggttt aatgctagat
    18781 atgccgacaa agaaccctaa aacacgtaag aaaccaaaag tcaaagtcgg tgacatggtt
    18841 cgctgtgaag cagaagagtt catttatccg tttcgtggat atgtagaaca cgtctataat
    18901 cactcagcga tcattcgtat tgaaaacaca atggaatgcg ataagtggtt agcgaaaagc
    18961 aaagagaatt tagctgtagc gagattggtg gatatggagg ttataaacaa tgaagtttaa
    19021 aatctttgaa gaggacactc gttataaatt agaaaaagaa ttaaacgatt ttgcgaaaaa
    19081 caatgagatt cagcatatat ctttagcaac ttctaagaga ggttatgcaa attactatgc
    19141 agctgttgtg agctatgtaa gtcgagaagt gtaactcggc aaataaaaaa agccggatcg
    19201 ctccgactga ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact
    19261 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
    19321 tgaagaactt tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
    19381 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
    19441 ttcaaatgat ggatatagaa gcggagagag acgcgattct ag
//
LOCUS       DACCCS010000050        19457 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.051, whole genome shotgun sequence.
ACCESSION   DACCCS010000050 DACCCS010000000
VERSION     DACCCS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.051"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            133..3660
                     /locus_tag="IVK82_001967"
     CDS             133..3660
                     /locus_tag="IVK82_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP6393014.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IVK82_001968"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IVK82_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP6393015.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IVK82_001969"
     CDS             7448..7789
                     /locus_tag="IVK82_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6393016.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IVK82_001970"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IVK82_001970"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP6393017.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IVK82_001971"
     CDS             9146..11566
                     /locus_tag="IVK82_001971"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP6393018.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IVK82_001972"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IVK82_001972"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP6393019.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IVK82_001973"
     CDS             13000..13737
                     /locus_tag="IVK82_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6393020.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IVK82_001974"
     CDS             13751..14566
                     /locus_tag="IVK82_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP6393021.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IVK82_001975"
     CDS             14579..15226
                     /locus_tag="IVK82_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6393022.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IVK82_001976"
     CDS             15242..15898
                     /locus_tag="IVK82_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6393023.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IVK82_001977"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IVK82_001977"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP6393024.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IVK82_001978"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IVK82_001978"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP6393025.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IVK82_001979"
     CDS             18240..18758
                     /locus_tag="IVK82_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP6393026.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IVK82_001980"
     CDS             18865..19365
                     /locus_tag="IVK82_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6393027.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3341 c         4178 g         5397 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagc
//
LOCUS       DACCCS010000051        19309 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.052, whole genome shotgun sequence.
ACCESSION   DACCCS010000051 DACCCS010000000
VERSION     DACCCS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.052"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            968..2218
                     /locus_tag="IVK82_001981"
     CDS             968..2218
                     /locus_tag="IVK82_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP6393028.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            2237..3247
                     /locus_tag="IVK82_001982"
     CDS             2237..3247
                     /locus_tag="IVK82_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAP6393029.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDVHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFEDGGTRVIHYTPETYIEKQ
                     NG"
     gene            3257..3910
                     /locus_tag="IVK82_001983"
     CDS             3257..3910
                     /locus_tag="IVK82_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6393030.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            3929..4927
                     /locus_tag="IVK82_001984"
     CDS             3929..4927
                     /locus_tag="IVK82_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP6393031.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWKPTVSLEEGLRESYNWIEKQIKG"
     gene            5003..5764
                     /locus_tag="IVK82_001985"
     CDS             5003..5764
                     /locus_tag="IVK82_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAP6393032.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            5782..6888
                     /locus_tag="IVK82_001986"
     CDS             5782..6888
                     /locus_tag="IVK82_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6393033.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRDVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            6878..7111
                     /locus_tag="IVK82_001987"
     CDS             6878..7111
                     /locus_tag="IVK82_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393034.1"
                     /translation="MINDMEVTKLMALRNRYALNIVDNCTRKIAKILGCCIGKGAQIG
                     NSVEFVHNSVGTVIHSDTILEDGVKVYQNVTCG"
     gene            7310..7765
                     /locus_tag="IVK82_001988"
     CDS             7310..7765
                     /locus_tag="IVK82_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAP6393035.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            7779..8552
                     /locus_tag="IVK82_001989"
     CDS             7779..8552
                     /locus_tag="IVK82_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6393036.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            8539..9669
                     /locus_tag="IVK82_001990"
     CDS             8539..9669
                     /locus_tag="IVK82_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6393037.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            9700..10935
                     /locus_tag="IVK82_001991"
     CDS             9700..10935
                     /locus_tag="IVK82_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393038.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFVPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLSVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            10960..12069
                     /locus_tag="IVK82_001992"
     CDS             10960..12069
                     /locus_tag="IVK82_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393039.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAKYEKYMDFVWEE"
     gene            12111..13445
                     /gene="gmhB"
                     /locus_tag="IVK82_001993"
     CDS             12111..13445
                     /gene="gmhB"
                     /locus_tag="IVK82_001993"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAP6393040.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            13480..15117
                     /locus_tag="IVK82_001994"
     CDS             13480..15117
                     /locus_tag="IVK82_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP6393041.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            15219..15614
                     /locus_tag="IVK82_001995"
     CDS             15219..15614
                     /locus_tag="IVK82_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP6393042.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            15632..16363
                     /locus_tag="IVK82_001996"
     CDS             15632..16363
                     /locus_tag="IVK82_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393043.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            16387..17838
                     /locus_tag="IVK82_001997"
     CDS             16387..17838
                     /locus_tag="IVK82_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP6393044.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            17864..18475
                     /locus_tag="IVK82_001998"
     CDS             17864..18475
                     /locus_tag="IVK82_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6393045.1"
                     /translation="MIDIISAIMVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            18657..19172
                     /locus_tag="IVK82_001999"
     CDS             18657..19172
                     /locus_tag="IVK82_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-keto-6-deoxy-D-glucose epimerase"
                     /protein_id="HAP6393046.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNGERLSF"
BASE COUNT         6223 a         2699 c         4218 g         6169 t
ORIGIN      
        1 ttaccaacat gtttagacat tttcctctag gaaaaaatta actagcacca cgggtgatat
       61 atatgtatat ggtgactgaa gacaaaaaaa cagaactaat gaaactgaat aaaaatatag
      121 agtgaactag tttctaagag ggattttaaa ctagatttca ttggtaataa aatttggaga
      181 gatggaaaaa cagcacttaa ggaagcagat tttgttgtca tttttgttcc tacaaactat
      241 ggtagtgaat gtgttattga attagtgatg tctgttattt cagaggcatt ggggataatt
      301 aaatccacta tacctgtagg atatacaaaa agtggaagaa aaaaatatta tcaatagatt
      361 ttatttagtt cagaattttt acgtgaaagt agattattaa tgataatctt tatccaatta
      421 gaattattat cgtaggatgt gatgatgaaa gccgtgaaat tgttcaagta tttaccaatg
      481 tctccctgcc agtaaatccg cttagaattt gagccttgaa aatgtgcaat gataaaaaac
      541 aaaaactcgc tcaaaataga cagtttgggg tgggaataga ccaataatgt accagacaaa
      601 cttccatttg gggtgaaaat aggtcaataa agcgatgtta aacatacctt tggagtaaga
      661 atagagggta ataagtgaat ttataaactt tttggtgact ggttgagtgg aaaatgtagg
      721 tacatttcaa tgcgaaaatg tattataacc ccaaacgagg tcaataaaaa tgactcaaac
      781 ttgcaatata accctaaacg ggtaggggat ttatgaaaaa ttgaatttgc cggcgtagga
      841 gctggctgtt ttaagtatga atgaacacag ctttcgggct gttttatata aatagcaggt
      901 tactgttcac aaacagcatt ttgttaagaa agtagtgacc acagcagtgg tggaaagagg
      961 aaatattatg aagaaacagt ataacattgc agtcgccggc accggttatg ttggtttgag
     1021 tattgccact ctgttggcac agcaccataa ggttacagcg gtggatatca ttccagagaa
     1081 ggttgaactg attaataacc gtaaatctcc aatccaggat gattatattg aaaagtacct
     1141 ggctgagaaa gaactgaatc tgaccgcaac attggatgca aaggcagcat accagtctgc
     1201 ggattttgtt gttattgcgg ctcctactaa ctacaatagc cgcactcaac attttgacac
     1261 atccgctgtt gaggctgtta ttaggctggt catggaagta aatcctgatg ccattatggt
     1321 tattaagtcc accattcctg ttggatacac cgcttctgtt cgtgtaaaga ctggtagcaa
     1381 gaatattatt ttcagccctg agttcctgcg tgaaagtaag gcactgtatg acaatctgta
     1441 tccttcgcgt attattgtgg gtaccgatat ggatgatgag cgtttggttg aggatgcaca
     1501 cacttttgct acactgctac aggaaggcgc aatcaaagag aatgtggaca cattgttcat
     1561 gggctttact gaagcagaag ctgttaagct gtttgccaat acatatctgg ctcttcgtgt
     1621 cagctacttc aatgagctgg acacttatgc agagatgaaa ggcttgaaca ctcagcagat
     1681 cattaatgga gtttgccttg atcctcgtat tggtactcat tacaacaacc cttcctttgg
     1741 ttacggtggc tattgcctgc cgaaggatac caagcagctt ctggccaact acgcagatgt
     1801 tcctgaaaat ctgatcgagg caatcgttga atctaaccgt actcgtaagg actttattgc
     1861 ggatcgtgta cttcagatgg ctggttatta caattacgca gaagcaaatc agtatgatgc
     1921 tggcaaagaa aagcctgttg tgattggtgt ttaccgtttg accatgaaat ccaattctga
     1981 taacttccgc cagagttcta tccagggcgt tatgaagcgt gtaaaagcta aaggtgcaac
     2041 tgttgtgatt tatgagccga ccttgaaaga tggagagact ttctttggca gtaaggttgt
     2101 gaatgatttg gaacagttta aggcaatgtg tgattctgtt atagcaaatc gctacgatgc
     2161 ttgccttgat gacattaagg aaaaagtata tacccgtgat atcttccaga gagactaaga
     2221 ttaggagaga gtatttatga ttattacaaa aacaccgttt cgtatgtctt tttttggcgg
     2281 tggaacggat atggaggatt atttcaaaga gaatggcggt gcagttcttt ctactacttt
     2341 tgataagtac tgttatgtga atgtgagaca tctgcctaga ttttttgatt acagcgctga
     2401 gttatcttat tctcgtactg agagagtaac tagcattgaa gatattgagc atcctgcgat
     2461 tcgtaatgcg atgaaaatgc tcgatgtgca tgaaattcgg cttacatacg aagctgattt
     2521 acctgctcgc tctggtctgg gtacatccag ttcttttgca gttggtatgt tgaatgcttt
     2581 ttatgcactg gagggtaagt atgctgacaa gaagaaactt gctgatgaag ccatttatct
     2641 tgaaagagtt ctgtgtaatg aagccggtgg atggcaggat cagattgccg catcttatgg
     2701 tgggttcaat cgaattaatt tcaatgtgga tggttatgag gtgctgcctg tcattatttc
     2761 gcctgaacgt aaaaagcagc ttaacaacaa tctgatgatg ttctttaccg gattcactag
     2821 attttcttct gatgttcaaa aggctaacaa cgtcagtggc acagaggata agagagtaag
     2881 actgaaaaag atgtatgagc ttgtagatga agctgaggca attcttactg ataagaacag
     2941 aaatttagat gatttcggta gacagcttga tgttacttgg agattgaaaa aaggtactgg
     3001 tggagcaatt agtacaggaa gcattgatga actatatgaa aagggtatgg ctgcaggtgc
     3061 tttaggcgga aagttgctcg gtgcaggtgg tggaggtttc ttggttttct atgtacaacc
     3121 tgagaaacag gatgcagttc gttgggctat gcgtgatttg atgtatattc cgtttgaatt
     3181 tgaagatggt ggaactagag ttattcacta cacgccggaa acttatattg aaaaacagaa
     3241 tggataagga gtaacaatgt tagcggaaaa acttaataaa catattgact tattgattaa
     3301 acgatatcct acacttgaga cgattaaaca ggacattatc gatggatacc taattatgga
     3361 ggaatgctat ttgaatggtg gtaaacttct tgtggcagga aacggaggat cagccgctga
     3421 ctcggaacat attgctggcg agttgatgaa gcggtttaag acgccacgtc ctgtaagtaa
     3481 ggaatttgcc gataagttag ttgcaattga ccctgtaaga ggtccggaat tagccaaaaa
     3541 tctcgagtgt ggtttaacgg caattccttt ggtcgcacac gaagcattgt ctactgctta
     3601 tattaatgat gtcgatggtt tgggtgtctt cgcacagcag ctgtttggct ttggtagatc
     3661 tggtgacgta ttccttggta tttcgacgtc cggaaatagc aagaatatta tgagcgcaac
     3721 tgttgttgct agggctctcg gtataaaagt tattggcttg acgggcgcaa agggtgggga
     3781 acttgccacg gtagcagatg tttgtgttaa ggctcctgaa gttgaaacat acatgattca
     3841 agagtatcac ttgccgatat atcattgttg gtgtttgatg ctggaagata aattctttgg
     3901 aaacgaataa aatttggagg aatgttaaat gaaaaagaca attatggtta ccggtggttg
     3961 cggtatgatt ggctccaatc ttgttaagag actggtgaag gaaggctgct gggatgttta
     4021 tgtagctgat aatctgtgga gaggaaagtt ggagtacctg aacgacgaag atgggcatcc
     4081 tgtcatcgac ctggatactc atttcttcaa tgctgatctg actgattatg agcaggctaa
     4141 aaaggttatt ggtacgactg agtatgtagt tcatctggct gatgttgtgg ctggcattga
     4201 ctatgtgttt aagaaccagg gcgaattgtt ccgaatcaat aatctgatca attctaatgt
     4261 ttttgattgt tgccgtaagg ctggcaagga gaaaatcaag ggtgttatct atgttggtac
     4321 cgtatgctcc ttccctctga ccagacagaa tactttgaat cctgagcctc tccgggaaga
     4381 ggagttgttc cctgctcttc ctgagtctgc ttatggctgg agcaaactga tgggccagtt
     4441 ggagatgggg tatttggaaa aagaaaccgg aattccgtgc tgtatcctga tgcttcataa
     4501 tgtttatggc acacctactg attttggcga acgtagtcag gttattcctg ctctgattcg
     4561 taaagcaatc aataatcctg aagagccttt taatgtgtgg ggtagcggag agcagggacg
     4621 tgctttcatt catgtcaatg atatcgtaaa tgcattggtg ctcgccctgg ataagggttg
     4681 gggtcatggt catattcaga ttggcccttc tcactgtact tccatcaagg aaattgcata
     4741 taagatcatt gaaatttctg gcaaagacat caagcctttc tttgatactt ctaagcctga
     4801 aggtgataag gctcgttgcg cagattattc taaggcgaaa gagattttgg attggaagcc
     4861 tactgtttct ttggaagagg gtcttcgtga atcctacaat tggattgaaa agcagattaa
     4921 gggttaataa gtatataagc tgttcttcct ccgtgtaatt agaggatgcg gaggaagaac
     4981 atatgagtaa ggatgtactg agatggaaaa gaatatgtat aaaagaaaac gtgatttcac
     5041 tgtgtgtgga atagtaggtg tttgtgctac tacaaagaac gagtttgtaa gggaagtttt
     5101 ttccatgcca acatctccgg taagtgcgac tgttgatctc gtgggagtgc cggccattat
     5161 ttcggcaaag gaaaatccca aaattgctga gatgtatagc caggctacaa tggctgcaat
     5221 agatggtatg cctattgtta aaattggacg gcgcaaagga tttacctgtg aacgatgtgc
     5281 tgcaccagat attatgggaa tggtttttga agaaggcgtt agacagggaa aaacacatta
     5341 tttctatggt ggaaaagata acgaagtgtt aaataagctt cgtgcaaacc tagaaagaga
     5401 ttacccggga atcaaaattg ttggaatgta ttccccacca tttcgtccgt taaatgaaga
     5461 agaggatagc aagatttgcg aggaaatcaa caacttaaat ccagattttc tgtgggttgg
     5521 tattggtgcg cctaagcaag aaatgtggat gtgggaacac cagaagaaga tccataacac
     5581 agtaatgttc ggtgtaggcg ccggctttaa cttttttgcg ggcactttgg aaaaagctcc
     5641 tgaatggatg gaaaaagcta gtctagagtg gttatttaga ctggtaaagg aacctcgtag
     5701 actatggagg cggtatatct tgggaggtat aaagtatatc tactatacta ttgaatctaa
     5761 gtaggggggc aataaaaata gatgaaaata cttatagtag ttacaatgac gaataacttt
     5821 ggtaagaagg ggtcctataa ttctcaggaa ataggattag ctcgagggtt agctgaatta
     5881 ggccacgaag ttattgtata taaaagtgta aaaagtgatg aggaagttgg aacagagcaa
     5941 ttaagcaata cggcgatgat tcactatatc aagtgtcgtt atgtaggatc aaatggattc
     6001 ttatcaacaa agaattttga taagaatgcg gatgttctaa tacaatttgc ggaccttcaa
     6061 ctatcagtac ctcatgtata ctcatggact gtaaaaaatg gaataaaata tataccttat
     6121 aatgggataa cgcgaagtca aagtggcaat aaatatataa agacaattat tgaaatattg
     6181 gcgcatcgaa attataaaat ttataagaaa aatgggtgtg ttgccaaaaa tgatcgagtt
     6241 tctgaaaaat tagtacaatt aggagtaaat aaagtgaaac ttgctcctgt ttgtgtggat
     6301 tttgaacagt tacataagaa ttacgaagca atagatttta ctactctcag agaaaaatgg
     6361 aatattcctc gcgatgttcg tgttatttta tatgtaggac ggtttgaaaa ggataaaaga
     6421 ccgttagata tgattagttt atttaataaa cttgatgagc ataattattt gctgatggta
     6481 ggaactggtg ctttactcga gcaaacgaag cagcagataa atttacttgg actgcagaat
     6541 agagtaaaaa taattgagcg tattcctaac aaagatattt gggagttata ttgcttatcg
     6601 gatgtatttg taaatctaaa ctatactgaa atttggggta tggttttgct agaggcaatg
     6661 tactatcgcc tcggaattat tgctgttaat gcacctggcc cacagaatat tattttggat
     6721 aatagatatg gaatgttggc taaatctgac gatgaaattt tgacattctt aaaagacgga
     6781 cgatttacca acgatatcat agatgcagca tatcagcgtg taactacaga atttagatgg
     6841 tcaaagtcgg gagaaattat gcttagcatg ttggaaggtg ataaatgata tggaagtaac
     6901 gaaattaatg gcattgagga atcgatatgc tttaaacata gttgataatt gcacgagaaa
     6961 aatagccaaa attcttggtt gttgtattgg taaaggtgca cagataggga actctgttga
     7021 atttgttcat aattctgtag gcactgtgat tcattctgac acaatccttg aagatggtgt
     7081 taaagtttat cagaatgtga cgtgtggttg agcagatagt aatgaacgta tcatcattcg
     7141 agtaaaaaag aacgctgttt tatgtgtggg tgctaaaatt ctttgtaaat ctaacacaga
     7201 attatgcgtt ggggaaggtg ctgtagttgc tgcaaataca gtggtattga ggtctattcc
     7261 accctacgaa gtgtgggggg tacctgctaa aaagatgggg agataaaaca tgaatgcata
     7321 taaattatac aaagtagaac gatggtttta cttacacaaa atggagtttt ttgccaagat
     7381 tgtaaaggca ctaatttatg tacttcataa tagctcaatt tcatatcaaa cagaaattgg
     7441 agataattgt aagttcttat acggtggcat aggttgtgta attggaaaag atacaaaaat
     7501 aggtaatcac gttataatcg gaacgaatgt actgactggt ggtagatcca ataagcctgg
     7561 tatgcctaca attggaaaca atgtatatat cgctactggc gcaaaaattt taggtaatat
     7621 cacaataggg gacaatgtaa tagtaggggc taatgctgtg gtcattaatg atgttgaacc
     7681 taattgttcg gtaggaggcg ttccggctag agtattgaaa aaagatattg atgtaaatga
     7741 ttattgtagt ttgaatcgtt actaaaagga gagattgtat gttgtatttt gttattcctt
     7801 taatgagtgc gaaggcagca aacaattggg agatggttag taccttattt aaccgaacat
     7861 tatggtcgtg ttataatcag actgatcctg atttcaaaat cattgttgct tgccatgaaa
     7921 ttcctaaact cacgcatgaa tatgatgaca gagttgaatt tattcaagtt agcgaaaagg
     7981 atgcccctat tcccacaaat caagacgaaa aaatgattga taagggttat aaaacacatg
     8041 ctctcgcaat gcgtttgcga gagttgggcg gcggtttcgc tatgatggtt gacgcagatg
     8101 atcttgtaag ttctcatttg gcggaatatg tgaattccca tccccaggaa aatggatttt
     8161 atgtcaaaac gggatacgtg tactttgttg gagacgatta tatgaaggtt ctaccaaagt
     8221 tctcatcagg aagtgcgtgt atagtaaaat atgcagtgga agacctgcct gaggcatatc
     8281 cggaagttat gacagcaaat tgtgatgata atccatggat cattagaaaa cgacatgggg
     8341 gcgttgttcc agcatgtgaa gagcaagggc gtccattaaa acctcttcca ttcaaagcag
     8401 cagtatatgt attaggaaca ggggacaatc actcgcttta tggtaaaacg acaaaatatc
     8461 agacgaagat gcgagagata ctggaaatgt ttatagcaaa acgtaaaatt aaaggaaaac
     8521 tgaaagcaga attttctgtt gattggttat aaggtgagta ttatgaaggt attgatgtgt
     8581 gaaagccaaa tgccagagaa ttggtattcg tttgatcttt ctaaagaact taatcaatat
     8641 gttgatttaa cattgctgtg taagcgtgat gttcctcctt tagatcctaa aattaagagc
     8701 aaacatattt tgtatcataa aagtacaaat aaagtgtttt cggcacttgt ttttctgaaa
     8761 agcttgttct taatttggaa ggaaataaga tttggtgatt atgatattgt tcatttgcaa
     8821 ttgatgaaaa gtctcaaata tgaaggcgga ttgtacgttt tatgtaagag acactataaa
     8881 aaattaatta tgacagttca taatgtgtta ccacatgagg ttcgtgaaaa ggatcgccaa
     8941 gagcacttta atatatattc aaattgtgat ggtttcattg ttcacaatcg agtaacagaa
     9001 cagcatttat atgctgaatt cccagaatta aatgcaaagt catatgtttg tccacatgct
     9061 atctatggag atgttaaaca tatatctaga gcgtatgata aagataagtt tgaatttatc
     9121 ttgtttggac aaattcgtcc atataaggga attgatgtac tgcttaatgc aatcacagaa
     9181 cttccaatgg atgtaagaaa gaaagttcat tttaccatag caggtcaaca acatcctaaa
     9241 caggatagta ctgactataa aaaaatgatt gaagatttaa aggtttcaga ttgtgttcta
     9301 tataagccag agagaatttc tgatgaagaa atggacagta tgttcagaaa tgcagatgcc
     9361 gcaatatttc catataaaga aatctatgga agtggagcgc ttcttatggc atacacttat
     9421 tgcttaccaa ctattgttag taatgtacct gcttttatgg aagaaaccga ggaaggaaag
     9481 atagggttac ttttcgaaaa tttgaattct aagagcctag cagacacaat tgtaaagtat
     9541 gtgaacctta gcccagctga gaaaagcaaa atagaggatg atatatatga gcttgtaaac
     9601 cgcaagtata cctgggcggc atctgcaaaa ctcacatacg aggcatacaa acaaattttg
     9661 aatgagtagt aaaacaatta gaaaggaata ataattaaca tgcagattag taaatggaaa
     9721 atcatacagt ttctaatggt ctttctaatg attatctcat caagcatatt gaagcctttg
     9781 ggcggctggt tgggagaaac gatgaacgtt agacttgtga cagtcattct agcaatagta
     9841 tgtttgttaa tagcctcatt accagcattt agaaaaaata gatatttaaa tttctgcact
     9901 ttactgattt gtgttgttat aatagcagct tacatctact cagataagac aatatgggtt
     9961 aaatctaggg aaagtatgag tttatactat gtattattgg cgtatccatt gttctgtact
    10021 ttaattaacc aagcgtggaa tttggattca atgctaaata cgatatgtgg tgtaacattt
    10081 ctttcataca ttcttagaac atctatttca cttattcaga aattttcagg agtacttctc
    10141 tatgaaaaca tatatcgtga gtgtgccccg gaaaattggt atcgtggagg ctttttaaga
    10201 attaatccac catgttttac aattataata ataccgattg ctttgtatat gattgagcga
    10261 caagttgtag ctagaagaaa agtaaaatat tatttactga ttgctagcgc actggcatat
    10321 tctgctgtta ttcatggttc tagatctgtc cttgtctatc aaattattgt acttggttgc
    10381 tatatccttt ttaagaaagg ttcttcgaaa agaaaaattg caatgtgggt tttagctggg
    10441 ttgcttgtag tcatatttat taattcgacc atgttctcaa catttgttga aactttcaca
    10501 aacagtacag gtgaatatgc aggatctgct gaatctcgat ttgcttcagt ttggtttttt
    10561 gttcctaaat ttttgcaatc gcctttgttc ggtactggta ttcttactgg tgaggaattt
    10621 acgtttatat taccaggagg agttagagga cacctatctg atattggatt tttctataca
    10681 attactcaat atggtgtagg tatacttata tttgatattc tgtttattat aaaagggttc
    10741 aaaacagcat tacttgcatc aaaagtgggt atgactggct atcaatattt agcatttgga
    10801 attacattaa gtgttttgct aacagggatt aatatagact ggttttatcc gatatttact
    10861 ccagctattc ctgtatgctt agcggttata gaatacatct atcaagaatg tagaagtgtg
    10921 gctaacatag aataggaaat attaatagga gtgcatctca tgaataagaa agagatttta
    10981 tataacatat tgaagaagta cgaaaattta ccatatgtgt ttaggaacat gtttggtcat
    11041 aatataagta aactcgtatc ttggaaattg actgctgatt gtgcaacagt agaagaaaat
    11101 tctgaagttt atgcagcgtt tcaattgcgc tcaaaggaca tgtctgatgt tccagtgatg
    11161 ggtttagtta attgttgcaa agatgtagca attgtaatgc aggggccact atgtttaaag
    11221 gatgatttct cttataatac tctggcctac tataagaaat gttatccaaa tgctttaatt
    11281 attctttcga catggaagga cgaagatatt aaggcattga aaaggataga ggatcttgga
    11341 gttatagtta tttgtagtga gaaacctgaa aagcctggac acttaaatat taattatcaa
    11401 gttggaaaca ctttgcctgg aatagtcaga gcaaagcagc ttggtgcaaa gtatgtttgt
    11461 aaaactagaa cagatcaaag aatataccat ccaaatgcaa tggcgttttt ctgcagttta
    11521 cttgaacagt atcctgttaa taacgaagat gattggaaat taatacaaaa tcagcgatta
    11581 ctgttactat caatgccgta tggagacatg tttttcccgt attgtttgtc agacttcctg
    11641 tattttggtg atgttgatga tatgataaac ctcttctcga ttcctagtga tgtgagagaa
    11701 aagggagctg tgtctagagg agtctcaaga agggagatat ctgaaaataa tttagctcca
    11761 gaagttcagc ttcttcgaag ctatatttca cgaatggggg gcaatgagga gtgctctatt
    11821 agggcatatt gggagtttgt aaaaaaccat gttattacta ttaacaaaaa tcaaattgat
    11881 ttgtactggc ataaatatgt aggtcgttat tcaaataata ctatttatgg tacgtattat
    11941 attgatgata gccaagatgc acttcactgc tataattttg attttataaa ttggttgaat
    12001 ttgtacactg gaaaatacga atacaaagca aaatatgaaa aatatatgga ctttgtttgg
    12061 gaagagtaat taacttttat agtaaaaagt actttaggga agggcatatt atgaaaacag
    12121 taattatggc gggcggacga ggaagccgaa tttcggaatt gtttccggat attcctaagc
    12181 caatgattcc tatagatggc attccagttt tagaacgaga attgtgttcg cttagagatc
    12241 aaggctttaa tgatattatt ttgactgttt cctatttgca tgaaaaaata gaggagcatt
    12301 tcggagatgg ttcaaaatta ggaattaaaa tcaaatattt tattgaaaaa actccacttg
    12361 gcaatgcagg tgcattgtat tttttgttgg atgaactggg tgatgaaccg tttttacttt
    12421 tgaatgctga tgcagtattt gatgttgatt tcaggcagat gattagattt catgttgaga
    12481 ataacgcaga tgttactttg tttacgcatc ctaactcaca tccgtatgat agtggattaa
    12541 ttatatccga tgacaataag aaagtgcttc aatggttagc gaaggaagat caaagacctg
    12601 aatattataa aaaccgagta aatgcaggtc ttcatgttat aaatccgaag gtgttgagag
    12661 aaaatccatt aatggaaatt gtaaataaat caatagttga aggtgggggg ctaccataca
    12721 aaattgattt agatagaatg attctgaaac cattggctaa cacagggcgt atgtattgct
    12781 acgattctcc agagtatgtt aaagatatgg gaacaccgga ccgatataga gctgtaatta
    12841 aggactttca aaagggaatt attgttggta aaaatctatc caatccacaa aaagcaatct
    12901 ttttagatag agatggaacg attaataaat acgttggttt tttgagaaat gcagaagaat
    12961 ttgagttaat tgatggagta tcggaggcaa ttaagattat taattctcta ggttttctgt
    13021 gtattgttat aacaaatcag cctgtaattg caagaggtga agttactgtt gatgagctta
    13081 atagtattca caataagctt gaaacaatgc tagggcatgc tggtggattt ttaaacggta
    13141 tttattactg tccgcaccat ccccataaag gatatgcagg agaggttata gaattaaaaa
    13201 ttgattgcaa ttgtcgcaag ccaaaacctg gactgttatt agaagcatca aaggatttta
    13261 acataaacct aagtcaatca tttatggttg gagatagtga aagtgatgta attgctggaa
    13321 aaaatgctgg ttgtaagaca gtactgatta cagaagacat caccttgcct gctaattatg
    13381 gccaagacta tagtgtctgt tcattgttag aatttgttaa aaatcattta tgcactatgc
    13441 aataattggt ttagtgaaac ttgtaaaagg agtaattaga tgaaggtatt aattccagca
    13501 gcaaaagttg ttgaaaaaga attgcagagt ataggaatgt taccagcaat tatctatcca
    13561 attaataaaa aaattgtctt tgattatttg tataatcagt ataatgcaat ctgcgattca
    13621 ttggatatta tctgttatga aaaagcggat aaggttcata gaagactgaa aaactatcat
    13681 gattccagaa ttcatatatg tgaccttcct gaattagggg atctagggca aaccgtgtat
    13741 tttgggttat cagatgaaga tgagcctgtt ctaattaact tcgcagatac aattgttatg
    13801 gagagtatgt ctgatataga aatggataca ttctattatt ctgaagattt tgtgtcgagt
    13861 acttggacat actttaaaat taatgaagga ataattactg atatccacga taaagaggaa
    13921 gcttacgtat ccgataaacg tgaaaaatta tttattggcg tatttatgtt ttcttcgtca
    13981 aaacttctga agagttgctt aagagaggca gttgagagtc ggaaacaggg gggaataaac
    14041 tcattttatc gggctttaca gctatacagc gaacgttgtc cgctaaaggc cgtaagaaca
    14101 gaaaagtggt ttgatattgg acacatcgat aaatattaca atacaaagtt ggaagttaaa
    14161 gcacgagagt ttaatcatat ctctatagat aaagatcgag ggatattgaa gaaaactagc
    14221 gatgacaagg ataaatttat tggagaaata aagtggtatt taaaacttcc tgctgatatt
    14281 gaatatgtaa gacctagaat ttttgattat tctacatctt acgtagctcc ctatgttgat
    14341 atggaatatt atgcatatca tacggttcat gagttatact tatatggtga tttaaaatat
    14401 aaccagtggc atgatctgtt tagccgtata aagtttatct gtagcgattt taagcgatac
    14461 accttaaaag atgaaaagat tagagagtca ttggaagata tgtatttgcg aaaaaccatg
    14521 cagagatttc aaaaattgtc tgaagataaa agattttcag ctttttttga gaaacaaatt
    14581 caaattaatg gtcgtaagtt tatttcttta accggtgtaa ttgaacttct aaagaatctt
    14641 atcccaagta tgctctatga tgtaaatgaa ttttgtatta tacatggaga tttgtgtttt
    14701 gctaatatga tggtagattc caattgttcc tttgttaagg ttattgatcc taggggaaaa
    14761 tttggaaaat atgatattta tggggacagc agatatgaat tagcaaaact atttcatagt
    14821 ttagatggta aatatgatta tatcatcaag gatctattta ctgtagaata tgataccgag
    14881 tgtgcagtga tagattatac aattaatacg cgtgtaagag attacgattt atttgaattg
    14941 tttaagtcgg tcttttcgga cgagattaag gacgattttg ataagattgt attgatagaa
    15001 gcgttgctat ttcttagtat gattccatta cacggtgaaa gttttgatca ccaaatagtg
    15061 atgcttgcta caggtatgga acttcttgca agagtaagta ataaagttat actctaatat
    15121 aaattgtgcc ttaatgaata acggttacaa tactgtttta gcaatgtagg cattaaggca
    15181 cgattatatt aattaataca atagaaggga agagaaaaat gtatagtgaa tattcttttg
    15241 tgtttgatat cgatggcact ttgtgtccga taaagaagaa ggatgagcga tatgaggatt
    15301 tggtgccgta tgagaatatg gttgaaaaac tgagattcta taaggagaat ggtgctaaaa
    15361 ttacattgtt cacctcacgc aatgttaatt cctataaagg aaatttggga atgataaatg
    15421 ctaatactgc aaaagtgctc ttggcgtggt tggataaatg ggacattccg tatgatgaaa
    15481 tcctgtatgg taagccttgg ccggggcata aaggcttcta tgtagatgat cgaacggtta
    15541 gacctgatga gtttttgaga tgctctgtag atgaattaaa tgaaatctgc aacagttcaa
    15601 aaaaaagatt ctaaataagg aggttagttc tatggaaaaa aagatgacag ttatcattac
    15661 gatggcaggt ttgggaagtc gttttcgaaa aattggttat gcggttccta aatatatgat
    15721 taatgccaaa ggaagaactt tatttgattg ggccatggat agtttggttg gatacaataa
    15781 aaatgttgat aagtatattt ttgttgttcg tcaggaagac aacgcttcag cttttataaa
    15841 ggagcattgt gcttactttg gtattgaaaa cgtagaagtg attgaaatag actatatgac
    15901 agatggtcaa gcaactacat gtatgttggc tattccttat tgcaatcaag acaatgagat
    15961 tatggtttac aatattgata catacgtgga atcaaatgag atgaaatacg aagatatttg
    16021 tggagatgga catattccat gctttcatgc tgatggcgat cattggagtt ttgcaaagct
    16081 taacgatcga aatgaagtaa tagaagtccg cgaaaaagtt cgcatttctg ataattgtac
    16141 tcttggtgcg tattatttct catcggcaaa gctgtataaa gctttatatg aagagtatta
    16201 tgcagactcc tctaatatgg agaagaatga aaaatatata gcaccattat ataactttat
    16261 gataaaaaaa gggttaaagg tgactattag catagtagat gcgaaaaagg ttcatgtgct
    16321 tggtactcca gaagagctca atgtgttttt gaatcaagaa taaatagtgt ttaggagaaa
    16381 atagcagtga ccagtaataa tctaattgta aaaacattaa gcagatataa gaatactcca
    16441 cttcctgtta aggctgcatt atggtttatg gtatgtggtt ttttacagag aggggtttca
    16501 atgattacaa ccccaatttt tactagagtt ttgtctacag aacaatacgg tttatacagc
    16561 acttatctat cgtgggaatc cttattgctc atgattacat cgcttagttt gttcaaagcc
    16621 atgatgaatc tttatgtaaa atatgataat caagaacata ttctttcgac agtatccgga
    16681 ttgactttag tactgactat tttttggttt gttattgcta tttttattaa ggatatattg
    16741 gcagaaattt taggattgac accagttcta gttgtctgta tgttctcata ttttatattt
    16801 caggcagaaa tacaatgttg gtctttacac aaacgatata tatatgatta taagacttta
    16861 ttagtagcta cccttgcagt tactgttggc tcatctgtag ttggctgttt agcggttctg
    16921 tttatatctc ctaccgctga ggcgagagcc ttaatgaatg tcctagtgac tggtgttgtt
    16981 ggcgtggctg tgtatatatc ggtatttaga agatgtaagg attttaagga taatgaagtg
    17041 tggaatttcg cagtagtatt ttgtgtatcg ttgatgccac actatttatc tgaatttatt
    17101 ctgaatggtt ctgataaaat tatgataaat tacttatgtg gttcgtcaga tgttgctatt
    17161 tacagcgtgg catattctat tggtgctcta attaaccttg ttacaaattc tgtcaattcc
    17221 acttttgcac cttatcagta tcaaaaaatc aagtcaggag aatatgattt actatcaaaa
    17281 agagctaatg aagttcttgg tttggtagcg gctatgcttt ttgcattaat gctatttagt
    17341 aaagaaattg tgttgatatt tggtgggcag aaatatatcg acagtgttaa tttaattgta
    17401 cctatatgta tcggtattta tttcaactat atgtttcagc tgtttgctag agtacaggaa
    17461 tactttgaaa agaaactcac tgtcgttatt ccatcggttt tgtgtgcaat tatgaatatt
    17521 gtactaaatt atgtttttat aaagttatat gggtatcagg cggtagcata cacaactgta
    17581 ttctcatata tgtttttctg cgtcatccat tatatttttt atagaaaagt ttgcaaagaa
    17641 ctattggatg gaaaaaacat atatgatgtc agaggaatac ttttgatttc tgcaatcacg
    17701 atagctggtg gcatcctaat ttcaataatt aataaatttg atttcataaa atatttaatt
    17761 atagcaattg cttgtgtcgc tgcaattata tttagaaaac gattgctact tttagtaagg
    17821 agaattatga gcaaataaac atttgctcat agagagatag agaatgatag atattatttc
    17881 tgctataatg gtaaatgtat taacagcatt atatcagccg ttcttatttg ccgtattaac
    17941 agcgtatctg tttatgtact ggtatctatt tatagttgat aactctcgag atcctaaaaa
    18001 cttaaaagat tctgtattaa tctggttgag atattttaaa gcatctagta aatttcgcag
    18061 actctttttt cttgcgtttt attttacttt gatattattt aaaaccttgc ttaatcgaaa
    18121 tatgtggatg aacccgcttt caaacgttat tggacagtgg tcattatata ttatagataa
    18181 tgctacagga gagagcagat tgacaacaga atgcgtggag aacacgatgc tgtttatccc
    18241 tttttgcatg ttattgttat ggtggcgtga cattaaatgt gatattatac gcaccatcta
    18301 tgttgggatt aagtatgtgt ttttattctc attaagtata gaatttacac aactgttttt
    18361 taggcttggt acatttcaac tatcggattt gttttataac accctagggg gcttaattgg
    18421 tgctttgcta tattggctat tctaccgatt aaataagtat atagacttaa aatagaactg
    18481 ctttgaattt taatatggtt aatatttttc tgcagacagg attgtgtaat ctcatcaaga
    18541 atactttaca tgccatctat ttttgctaaa acattgttgt tatatcttca agaggtaggg
    18601 aattttattg ctttaaatga ggtcgagcga attaaaaaat tggtaaaata tataacatgg
    18661 tggtagttat ggtaaaagtg tttgatataa ttaagttttc ggatttcaga ggatatactg
    18721 ctgttgcatt ggataatgag actcttttct cgattggtat tgagtttcac attaagcaaa
    18781 taaatcaagg atttagtcat aataaatata ctatgcgagg gctacattat cagagtggag
    18841 agcatgctca aggaaagata gtgatgtgcc tacatggtga aattatgaat atcgcacttg
    18901 atattcgaca aggatcttcc acttatggat gtgtgtatgt caacacattg agtgaaagca
    18961 atggtaaatg tgcgtatatc ccacgtggat atgctcatgg ctatcttact atggaggaca
    19021 atacgctagt tcagtggtgc gtagataatg atttcaattc taatgctgcc aaatgtataa
    19081 gttatgaaag cgtagatatt gaatggccgg tcagtcggca gtgtttcgtt ataagtgaga
    19141 aagacaaaaa tggggaaaga ttaagctttt gattaaaatg agctgtggag aaaagaagga
    19201 tactggaaaa taagaagaac aataaccgag caaatgctcg gttatttcta tgaggaatac
    19261 ataaaactaa atttagatat agatatttat tcacgaaaaa ctaatattg
//
LOCUS       DACCCS010000052        18789 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.053, whole genome shotgun sequence.
ACCESSION   DACCCS010000052 DACCCS010000000
VERSION     DACCCS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.053"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(534..1100)
                     /locus_tag="IVK82_002000"
     CDS             complement(534..1100)
                     /locus_tag="IVK82_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP6393047.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1100..2056)
                     /locus_tag="IVK82_002001"
     CDS             complement(1100..2056)
                     /locus_tag="IVK82_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP6393048.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2056..2712)
                     /locus_tag="IVK82_002002"
     CDS             complement(2056..2712)
                     /locus_tag="IVK82_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP6393049.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3082..5100
                     /locus_tag="IVK82_002003"
     CDS             3082..5100
                     /locus_tag="IVK82_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6393050.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5527..7941
                     /locus_tag="IVK82_002004"
     CDS             5527..7941
                     /locus_tag="IVK82_002004"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP6393051.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8289..9842
                     /locus_tag="IVK82_002005"
     CDS             8289..9842
                     /locus_tag="IVK82_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP6393052.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10223..12007)
                     /locus_tag="IVK82_002006"
     CDS             complement(10223..12007)
                     /locus_tag="IVK82_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6393053.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12022..13743)
                     /locus_tag="IVK82_002007"
     CDS             complement(12022..13743)
                     /locus_tag="IVK82_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6393054.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13843..14604
                     /locus_tag="IVK82_002008"
     CDS             13843..14604
                     /locus_tag="IVK82_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6393055.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15571..15839
                     /locus_tag="IVK82_002009"
                     /pseudo
     CDS             15571..15839
                     /locus_tag="IVK82_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16511..>18789
                     /locus_tag="IVK82_002010"
     CDS             16511..>18789
                     /locus_tag="IVK82_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393056.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAP"
BASE COUNT         6176 a         3337 c         3779 g         5497 t
ORIGIN      
        1 attgaaacat ccatctggaa tttatctttt ttactgcttt gatcataaaa gtctgcatat
       61 tcatcactga tattttttct aataaattct tccatcataa aaatcacctc aaaacgatta
      121 tacgaactta cgttcttgtt gtaaagcgat atttgagggg caaaaaaggg gcataatttg
      181 actgatatta agttagctat tgatatatga atgacataca aaaatataaa atcttttaaa
      241 aatgcttata tatcaatgtt tttgccattc ttttatatat ctgttatatg tcaaaaaaat
      301 gtgttcaatt tcaatttgta tttgaataaa gaggttgtga aaaagctgtc ctgcatcaag
      361 taataagaac agccaatcaa aaacagcttc tcatgttttt gatttggttg gacttattac
      421 cgcagatata agcttttgag caccgtgaat aaagaggtta tgaaatcaac gctctaacct
      481 gagtattaga gaaaaaaacg caaaacatat catttatgtt ttgcgttttt tatttatctt
      541 tttttctcaa tacaatataa aataggcggc tggttctttt ggttgataaa ccgataattc
      601 agcacattat atgtttcctg tggcagtttg gaacaaaaag aatcaaccat atccagttct
      661 ttttctccac cttcatgacc atagtagacg acaaggatca atcgtccgcg aggcacaagt
      721 cgtttcaata tttcttccat agctgtgcgt gtagtctctg gcatagtgat gattgcttta
      781 tcactttttg ggagataccc tagattgaaa atccctgctt tgagattttg ttgttcagct
      841 atcacttctc ctaacgtttc gtggccttgt agaaacaatg agacacgttc ttctaactga
      901 cgctgttcta gcctttcacg agtagcatcg attgcttgtt gctggatatc aaaactatag
      961 acatgaccag tttttccgat gtgttctgct agaaaaaccg tatcgtggcc attgcccatc
     1021 gttgcatcta cgacgtgatc ccctggttgc aagatttctt gcagtaatgt atggctgaaa
     1081 tgcaaagcag tttgcaacat cagacgctca cctttttccg tacatcatat tttccttgga
     1141 agctatctct gcgaagaagt tcttcatcaa ttgcattcaa gacttcccat tttttcaagc
     1201 tccacattgg tccaatcaag gaatcccacg gcgcatcccc tgtcaaacga tggatgatga
     1261 tctcttttgg tatcatttcc agctggtcgc aaatgacatt gacatagtca ggacggctca
     1321 ttaattgaag gcgaccttca tgatagtccc gcaacattct cgttttgcgc atcagatgca
     1381 tcaaatggag cttgattcct tggatatctg aatctaaaat ggtgcgtctg acattttctt
     1441 tcatcatttc taaactttcg cctggtaatc cgttgatcaa atgcgtacaa acacggatat
     1501 tatgtttgcg taatttagcc acgccatcta aataagtttg ataatcatgt gctcggttga
     1561 tcagttttga agtttcttca aaagttgttt gcaatccaag ttctacccat aaatacatcc
     1621 gctcgttcag ttcagcaagg taatcaacta cttcatctgg cagacaatcc ggtcgtgtac
     1681 cgactgataa tcctacgaca cctggtaaat tgactacttg ctcaaatcgt tctttgatta
     1741 tttcaactgg cgcatgggtg tttgtaaaat tttggaaata aacgatgtat tgttggacat
     1801 gaggccattt ttgatgcatc atgtcgattt ctttctggaa ttggattgga agagggtctt
     1861 caggtgcaac gatcatgtct cccgaaccag atacactgca aaaagtacaa ccgccatggg
     1921 caacagtccc atctcgattc ggacagtcaa atcctccatc gatcggcacc ttgaaaattt
     1981 tcccgccaaa ctcatttcgt aaagcgtaat tccaagaatg atatctttta tttccttctg
     2041 aataaggaaa agtcattaaa agtccttctt tctgtaagag ttatctattt gttttctttt
     2101 ttctctatac actggataag tcaggcacag ccaagaaaga ctgagtaagt agccgccgat
     2161 gatatcagta ggaaaatgaa cgcccagata aatgcggctt atcccgacac ttactataat
     2221 aaacatcatc agcaattgaa gcaatgtgcg taaggttctt ttttgaacaa aagctggaag
     2281 cagaaatagt aaagaaccgt aaagaatcat actgcctgct gcatgtccac tagggaaact
     2341 gtaacttgtt tcaactacta agtgcgccaa tgaaggacgc tctcgcatga aaaatagttt
     2401 gatcaccggg ttgatgattc cagcaattac aataaatcca gaaacgaacc aaaatgtttc
     2461 tgcccttttt ttcttatacc acatccataa agccgcacaa gcaaataaaa tgatgacggt
     2521 aagaggatta ccgaatttag tcataaagag ttggtagctg ttcagatcag gataagaagc
     2581 tctgacaatg cctgtaaacc actgatcgaa accagaaagc cacgacagat ggaactttac
     2641 gatccagccc aaaatgaaaa acaggacaag aaagaagcta ccagccacct gaaatgatgt
     2701 tttatatttc atacaattcc tcctttgaag caatgactta ttatagcatt tttggtccag
     2761 cgaaaaaaac ggtaaagaga taaaaccaat gtatgattcc tctttgaata accaatattc
     2821 ttataaaaaa aataactgta atcggaaaga cattttttgg taatgcaaat gaaatgttag
     2881 ggaaataaaa tcagtgtttt tcgaaatcct tttaaacaag aacgtagacg taggaagaac
     2941 ttgaaaatga acagaagttg ttcattacgg taaattacaa tatgattaca aaaaacaaca
     3001 aagctcttaa ataactttta ataaatatta agagaagtta taattattaa taatagataa
     3061 attgaagttg gaggatagaa gatggaaaac attgcaagaa aagaacggag acgtttagaa
     3121 cagaccaaac gttttcgaaa agcaaaacga ggggcagcaa tcgtgggaac tgcgatggta
     3181 ggctgttctg tcgctgcccc attagtacag ccggttcctg ttcaagcgga tgaaacacct
     3241 gttcagttcg gcgcaagaat caatacagcg gcatttatca cagaaattgc tacttatgct
     3301 caaccaatcg ctcaagccaa cgatctatat gcatcagtga tgatcgcaca agcagttgta
     3361 gaaagcggtt ggggcagtag tgcgttatct caagcacctt attataatct ttttgggata
     3421 aaaggcagct atcaaggaca aaccgtctac atggatacgc ttgaattttt aaataatcaa
     3481 tgggtaaata aaaaggagcc tttccgacaa tatccttctt ttgctgaatc tttcaatgac
     3541 aatgcctatg tacttagaaa tacttcattt ggcaacggtt attactatgc cggtacttgg
     3601 aagagcaaca cgaaatccta tacagatgcg acagcttgtt tgacagggcg ttatgcgact
     3661 gatcctggtt acgcaggcaa attaaataat atcatcgcaa cttacggatt gacccaatat
     3721 gatacaccag ctagcggaaa cgcaggcggt ggagcgacta caggtggcaa taatactgga
     3781 agtgcaggta gtactggcgg ttcaagcaat tctggtaata caaactcagg ttcagcaact
     3841 tcgaccactt atactgtcca atctggtgat tctgtttggg gaatctcaaa taaattcggt
     3901 atcacaatgg atcaattgat ccaatggaac aacatccaaa acaatttcat ttatccagga
     3961 caaaaactga caatcaaagg tagccaggaa aatggctctt cgacgaataa ttccggtaac
     4021 aataccaact cttcaggaaa tgctggtaca tcaaacggag gacaaacaac tggtgcgaaa
     4081 tacactgttc aatctggtga ttctgtttgg aagatctcaa atgatcacgg aattacgatg
     4141 gctcaattga ttgaatggaa caatatcaag aacaatttcg tttatccagg acaacagcta
     4201 atcgtatcaa atggcggttc ttctaatgct ggagcaaata ctggctcaaa tactgggaat
     4261 acaagtggct ctagttcgtc cagctcaagt tcttctaata cagcttcagg aacgaaatat
     4321 acagttaaag ccggagactc tgtttggagc gtctcaaata aatacggaat tacgatggct
     4381 cagctaatcc aatggaacaa tatccaaaat aactttatct acccaggaca acaactgact
     4441 gtttcaaaca gtggatcaac aaacagcacg tcaggtaata caggttcgtc aaccaataca
     4501 aataacagca gttcagttaa tactggcagc agttcgtctc aaggcactta taccgtgaaa
     4561 gcaggtgaat ctgtttggag cgtggcaaat aaaaacggca tcacgatgga tcagttaatc
     4621 gaatggaata atatcaaaaa caacttcatt tatccaggac agcaattgat cgttaaaggt
     4681 ggaacttcgg caaatacaaa tacaggttca actacttctg cagcgaaacc aaatacaccg
     4741 aatacgtctg ctacgacaag tacatcatcc actggtaata cgatgtatac cgtgaaagcg
     4801 ggcgagtcag tttggagcgt agcgaacaaa catcatatca cgatggatca gttaatcgaa
     4861 tggaataata tcaaaaataa tttcatctat ccaggacagc aagtgattgt gaagaaaggt
     4921 tcatctcaat caacaaacca acaatcagtt actggttcga aaacctatac tgtgaaatct
     4981 ggagaatctg tttggagtgt tgctgacagt catggaatta caatggctca actaatcgaa
     5041 tggaacaata ttaagaacaa ctttatttat ccaggtcaaa cgttgattgt agctaaataa
     5101 aatagctgtt cgaagaaata acgattgaat tccacaaaaa tatttgttac tataaggcta
     5161 cttaaatatg acaacacttg aaaaggaagt agtaaaaaga ctgaagtttc agagagcgca
     5221 tggctggtga gaatgtgcac ggatgctttt gaactcgcct ttgagttgcc cttgctgaat
     5281 acccgagtaa gcaagcggcg gtgacggccg ttacaacgtt cgaggccttt tcactaaggt
     5341 aaaattaggt ggtaccgcgt agattacgtc ctataaaaag agcaatcttt ttataggatt
     5401 tttttatttc ttttgtgaag tttgagaaaa caaagcaaaa ggaaataaaa ataaacagat
     5461 tctgccggtt ttttcgttat atttataata gaaacaagtg ttttcaatag aacaggagga
     5521 cagcaaatga gttacaatca taaagaaatt gagaaaaaat ggcaaaaata ctgggcaaaa
     5581 acgaatactt tcaacaccca tgatgatcct gaaaagccaa aattttacgc actggatatg
     5641 tttccctatc catccggtca agggctacat gtagggcatc ctgaaggtta tacggcaacg
     5701 gatatccttt cacgtgtgaa aagaagtcaa ggatacaacg tgctgcatcc aatggggtgg
     5761 gatgcattcg gtctgcctgc tgaacaatat gcgctagata caggaaacga tccagctgag
     5821 tttacccaaa aaaatatcga aacattccga cgccaaatca attctttagg atttagttat
     5881 gactggaatc gcgaagtgaa tacaactgat ccggagtatt ataaatggac acaatggatc
     5941 tttacgaagc tttatgaaaa aggtctagct tatgaagcag aagtagcggt aaactgggta
     6001 cctgaattag gaacagttat ttcaaatgaa gaagtgatcg atgggaaaag tgaacgtggt
     6061 ggctatgatg tagtcagaaa accaatgcgc caatggatgc tgaaaatcac tgcttatgct
     6121 gatcgcttat tggatgatct agaattagtt gattggccag aaagcattaa agaaatgcaa
     6181 agaaattgga tcggtcgttc agtaggagca aatattgaat ttaaagtggc tggtacggat
     6241 aaatcctata ccgtatttac gacacgacca gatactctat tcggtgcaac ttactcggtt
     6301 cttgcacctg aactagatct tgttagagaa attacgaccc ctgaacaaaa agctgctgta
     6361 gaagcttata tcgaagagac agctaaaaaa tcagatctaa agagaacgga tcttgcaaaa
     6421 gaaaaaacag gagtatttac tggtgcttat gcaatcaatc cagttaatgg caaggaaatc
     6481 ccaatctgga tcgccgatta tgtattatct tcatacggca caggagccat catggctgtt
     6541 ccagcacatg atgaacgtga ctttgaattc gctcaaactt tcggactaga aattcttcca
     6601 gttatcgaag gtggagatgt tgaaaaagct gcttacacag aagatggtac gcacatcaac
     6661 tctgaatttc tagacggcat gaacaaacaa gaagcaatcg ataaaatgaa tgaatggcta
     6721 gaagaaaatg gcgtagggaa aaaagaagtc agctatcgct tacgtgactg gctattctcc
     6781 cgtcaacgtt actggggtga accaatccca atcatccatt gggaggacgg aacagtcaca
     6841 gctgtcccag aagaagagct accattacgg ttgcctaaga caaaaaacat caagccaagc
     6901 ggtactggag aatctccatt agcaaatatc gaggagtggg tcaatgtcgt tgatcctgta
     6961 acaggtaaaa aaggccgtcg cgaaacgaat acaatgccac aatgggcagg aagctcatgg
     7021 tattacctgc gttatatcga ccctcataac aaaaaagaac tagcagatta tgaaaagttg
     7081 aagcgttggc taccagttga catctacatt ggtggagcag aacatgcggt acttcattta
     7141 ctatatgcac gattctggca taaattctta tacgatatcg gtgttgttcc tacaaaagaa
     7201 ccattccaaa aactatacaa tcaaggaatg atcttaggcg aaaacaacga aaaaatgtca
     7261 aaatctcgtg gtaatgtagt caatcctgat gatgtggtcg aaacttacgg tgcagataca
     7321 ctgcgcatgt acgaaatgtt catggggcca ttagatgctt cgattgcttg gagcgaaaat
     7381 ggcttagaag gaagccgtaa attcttggat cgtgtatggc gtttgatcgt tgatgagaac
     7441 aataaaatgc gtgaccgtat cactacattg aatgatggca agcttgataa agtgtatcat
     7501 caaacagtga aaaaagtgac agaagactat gaaaatcttc atttcaatac agcaatctcc
     7561 caattgatgg tctttatcaa tgaagcctat aaagtggacg ctctgcctta tgaatatatc
     7621 gaaggatttg ttcaactact cgcaccgatt gctcctcaca tgggtgaaga actatgggca
     7681 atccttggaa atgatggtgg tatttcttac gcaccatggc caacatacga tgaagcagca
     7741 cttgttgaag acgaagtaga agttgttttc caagtaaacg gtaaagttcg tgcgaaaagc
     7801 aatgttcccc gagacttagg aaaagatgag ctagaaaaag cagcattagc caatgaaata
     7861 gtgcaagaat atattgaagg caaaacagtc agaaaagtta ttgcggtacc aaacaagtta
     7921 gtcaatatcg ttgctaatta attggctctt tgtcaataag gactgatgag ttgtgcaaaa
     7981 tcaaatctgg gccagaatga acttcattct gtctcagatt ttttgttatt tcaaattgat
     8041 taattgattg atcaaaaagg ttttgatttt catgttctca aacacgttca atgtcttttt
     8101 cttctgaatg gtcttgtaag agatctctgt aatcatcaaa tttttgtcta ttaatcttag
     8161 tattttttga tgtataaact acctgaaatt gagagtacat accaaattca taaatttttt
     8221 attagtggga ctagtgattg tatggatttt gacgcataat gaatgaagta tgagaaaagg
     8281 agagacaaat ggatcaagaa ctatttatta tcgaagtatt atcagctaaa caaaaaatta
     8341 tcgagaaagc gaaaaaaaga gcgaaaagaa taatgttcaa gcttttaagc ttagtatttt
     8401 tctctggttt gctttttttc ggagctgtat cgttcctata tcgccatatg agtgcagtag
     8461 tccgttcagc tgaagggatg caaaaattaa ataatttgta tcacttttcg tggggagttg
     8521 gcggaataac aattgttgtg cttattgttt ttcttctact tatttggctc cgttaccatc
     8581 aattgtcgcg tcatttcgat caatggctta gtcaaagagc acaacagttt ttacataata
     8641 aaccaacttc tcaagatgat gactatcttt attttgagaa gacttcgacg agtcaagctt
     8701 ttcggataca aaaaagtgat tgccagttat tgctgagatc acttgatgat ttacctatct
     8761 acttaggcga aagcaaatca aaattcggaa tgaacacatt ccatattttt ttactagaaa
     8821 acgaacccag atgggaaaaa agccagtctt cgcctggtgg atacctgaca actaagatga
     8881 gtacattagt cactggtttt ttcctcattc ttttagctgg aacaatctat tatgggagat
     8941 acactcagcc aaaaatgtct tctcatataa atgctgcttc tcacatgaca atgaactcaa
     9001 ctaacgatac gcatatacaa aagacaaatg aagggttgtt agctgatcct aagcaaaagg
     9061 taaatttgac aacacaaaag ataaatgatc tcaaacttga tacagagacg cactctctct
     9121 atatgacaac agatagtggt gctaattggg tatttgtgcc gattgattcg caatggttgc
     9181 gttttggcga atacaccctg acaacggggc tgattcctga aggttattgg atggacaaca
     9241 catatgatat ttccgccgat ttctcatggt ttatctattc ttctggggat gatgtacgtc
     9301 tgctgacttc aagagataat ggtaaaacat ggcaaacgtc tattatcacc caatcagttc
     9361 agccgatacg ttatcgaaaa gcggtatttt atggcaacga tggtctattt atctgtacat
     9421 cagccagtgg gatgtctgca gaaactttgt acatttacac tactatggat ggcggaatca
     9481 cttggataaa aagtggaagt acaatgatca atcagccagt acaaaatgtc agcttcgtta
     9541 caacttcttt aggttttgtt tcgacacgtg aaagtttgtt ttatacaaac aacggaggac
     9601 gctcattcaa agaatcagtt ctgtcgattc cggaaaacta tcagacaggg gggatagatc
     9661 tttttcaatc accaaatgaa gttactcaaa taagcgcgaa tacgttagag acaaaatttt
     9721 atttgttgaa aaaaggacca atcgatgcag ggaaaatgtt tgcctgcctc ttccgttcaa
     9781 ctgataacgg ggaaacatgg caattcgttc agcaactatc gcaagtagaa caaggagatt
     9841 aaagaagaga gattgtgagc ctgacgttca gcaccgagtc acaagaaaca aattcaaaaa
     9901 acagcttctc atatttgttg aatagtgtaa ctcgtgattc ggagttgaca agagcaacaa
     9961 gaagcgattt tgaagtatag cttctcgtat tttacaagat cgtgtcttat tgccgatagg
    10021 ttgtctcttg aacaccgttt attcgtaaaa agggattgtg acaagtcttt tgtcactact
    10081 taccgaacga aactgaagga gctatccaaa aagtatcatt agtattattc tttgtgtttc
    10141 tagtggcacc actagtacat ttaaaaaagc tatcgatcat ggactaatca tacatgatcg
    10201 atagcttttt agataatgtt tgttacgcta tctgaaattg actattataa agactggcat
    10261 aaagcgttgg tgattggagt agttcagaat gtgttccttt ttcgacgata tcgccattct
    10321 tcattacaag gatcatgtct gcattttcaa tcgttgacaa acggtgtgca atcacaaaac
    10381 ttgtcctatc ttttgtcaca gcttccattg cttgctgaat caatgcttct gttcgtgtat
    10441 ctacgctaga agttgcttca tctaaaataa cgacaggagg attcgctaga atgactcgag
    10501 caattgtcaa taattgttgc tgtccttgag ataacgtgcc gttttcgcta gaaatgatcg
    10561 tatcatatcc gtctggcagt gttcgaataa agtgatcaca ttgtgcgatt tcagccgctt
    10621 cgataatttg ttctcttgtg gcatccattt tcccgtaggc gatattatca gctaccgttc
    10681 cttcaaacag ccaggtattt tgtaaaacca tcccaaaaga acgacgtagt tctccacgtg
    10741 aaagcttggt aatatcaaga ccgtcgaatt cgatctttcc tttattcagt tcataaaaac
    10801 gcatcagcag attgactaaa gtcgtttttc ctgcaccagt tggaccaacg atcgctaccg
    10861 tttgttttgg ctttactgaa aaatcgactt gattcatcaa tattttatct gatgtgtaac
    10921 caaactgtac atttttgaaa gctatttttc ctttcggttg ttctaaaagt cttgcatctg
    10981 gaaggtctgg aatttcatct gtttcatcta atatctcaaa tatcctatcg attgctgcca
    11041 ttgcggattg gatcgagttg atcacataag aagcggtaga aattggttca gaaatctgat
    11101 tgatgtactg aagataagct tgtaagaagc cgatcgtcac accacctgat agaacaagca
    11161 tagcaccgaa taccgcactt atgatgaagg cgagttgatt aatgaaacga atggccggat
    11221 agatagcaca gttcataaat tgagcacgtt taaaagcgtt gtattgcttt ttatttactt
    11281 cgtgtatagc agcttctgca tgagcttgct gattgaatgt tttgaccact agattaccag
    11341 ctaagatttc ttccattcga ttatttaaaa cacccagttc atactgattc tgttcagaaa
    11401 tctgtttgtt tttgttcgca atcttagttg taacaattga actgccaacg atcagcaata
    11461 agacgagcaa agtaagcttg ctttcgatat aaaacagcat cagaccagca gcagctattg
    11521 ttacgacaga agtaaaaaat tgattgatac ctgtcaataa cacttgagaa agttgattga
    11581 gaccggtagt tgtccgacta atgatgtcac ctacttgatg attgtcaaaa aacgacatcg
    11641 gtaatgtttt gaattttttt gtgatttctt ttcgcaccct caatgtgatc ttttcgctta
    11701 acgctgccat tgtccgttcc tgaataaatg aagtgatgct gcttaagact gaacagccga
    11761 tcagcaataa gacagggaac aacaatgctt gtttgatatc actgaaagaa agctgtccaa
    11821 tacctgtttt tcttatttct gataagagat catcgatccc gattcccatg atcaagggca
    11881 tggctacgac taataggttc ccgaataaac tgcatagaaa caatccgtaa aaatatttat
    11941 gttcaggttt gatcattttc caaaaacgta tcaaagcacg cgtgttcaac gtttttttct
    12001 tttccatcgt atcccttctt tctatattcc ttgtgatttt gcaaactcct gataagtttg
    12061 gcaattagac agtagttctt catgagtacc ttgtccaacg atttttcctt catctaaaac
    12121 aatgatgtta tctgcttgtt ggattgtact gaggcgttga gcaacaatca cgagagtttt
    12181 ctccgacatg tgtttatgaa gggctgctct taatgctgca tctgttttat agtctaaagc
    12241 ggagaaacta tcatcgaata catagatctc tgctggtttg atcaacgctc gagcaataca
    12301 tatccgctgt ttctggcccc ctgaataatt agaaccgcct tgagcaacaa agctgtccaa
    12361 tccgtcagga agggtgtcca ctagttcttc taattgagcg atcttgatcg ctttagacaa
    12421 ctcttctgtt gaggcatatt catttcccat ctgcaagttg cttaaaattg tcccgctgaa
    12481 taagaaagcc ttctgaggga cataactgat gtgggagcgg atctgttctt gagtcatttg
    12541 agtaagagga ataccaccaa gggttacttt tcctatcgta gggtcactaa gtctcaagag
    12601 aagtttcgcc aaactacttt ttccagatcc ggtcccgcca acgatcgctg ttgtttttcc
    12661 tttaggtatt gtgaaatcaa tatcttctag aactggatca gagatttcag tatatttaaa
    12721 tgtgacatgt tcaaaagcag ctgcatttgt ctgatcaaag gttggttcag ctactgtttt
    12781 gatcgtctct tcctcaattt cgatctctgc ttgcagtatt tcttctatcc gtcgtaatga
    12841 ggcaaacgat ttagggataa tgaccaatac catagctgcg atcatcaagt aagcaagtgt
    12901 cagcatagag tattcgacta cagcagtcac gacaccgatt tgtaatgctc cttctgctac
    12961 aagatttccg ccaaaccaca aaatagaagc ataaacgatt cccatgagca aaaaagcaat
    13021 tggagtgacc caagcaaata ttcggttcac tttgatcatt tgattagcat agtcagcaaa
    13081 gctcttattc gttcgctttt cttcatatga ttgattatca aaggcacgga tcatattaat
    13141 cccagtaaag aattctctca atgtgatggt gatccgatcc atttttggct ggatactcaa
    13201 agataaaggt gcccctcgtt tcatcagtcg ataaacaatc catacataaa aaacaacaga
    13261 gatcaatgta acccatgtta gtaatagaga ataacgaaac atcatgagaa tggtgaaaac
    13321 gcaaataacc ggtgctggta agatcatttg gcaaaataaa acaatcattt gctggacatt
    13381 atcgacatca ttcgtcattc ttgtcaaaag agatccagaa ctaaaacgac tgatatcctt
    13441 cattgacagc tgttggaatt tacggaaata tttctcccgc gtttggaatc caaacctagc
    13501 ggaagccaat gctgaaaagt aactagaggc aactgcagcg attgttccta gaaacgctac
    13561 aagcagcatt tcaactccaa ttttttttat aactgaggct tcgccacttg ctatacctgt
    13621 gtcgatcagc tttgctacta attttggtac acctaatgta ccggcgattt ctaaacagat
    13681 catcacaaag gtaaggagag cgagtccttt gtttttctgt aagaatgttt ttaagataga
    13741 catattattc ctcctttttt cctgatactt ttcgattcta taccttcaag taacttgaag
    13801 gtcaatagaa aaaagaataa actgaaaaag aggtgagaaa aaatgaaaaa aatgtatacg
    13861 attggccaag tatcggaact atttgatttg ccaaaatcaa cgatccgcta ttgggatgaa
    13921 caaggattaa tccgttcaag tagacaagaa gagaatgatt atcgattgtt tgatatcgat
    13981 gatattttta tgctttatga tatcgttttt tatagaaaat tagatatacc gatcaaacag
    14041 atgaagaatt tatatggaaa aacattgacg gagttatatg agacacttga tgaaacagaa
    14101 agaagaatcc ataaagaact ggtggtgatg aaacaaaaac aaaaagagat aagggagcaa
    14161 aaaaaacagt tgaaattgat gatcgataca aacgaggaag aatttcctgt agaagagatc
    14221 ccttttgact gtatgatctc aacggagttt gaagatattg tggaaatcaa aaaatttcta
    14281 cctaactatt caagctttgg gatgatgagt atgccagctt ccagttcaca gacgatgtat
    14341 ggatttttta tcgaccctag tgaagtacat ttatttactc aagacgtgat ttgggagaaa
    14401 aaagatacgg ctgtttatcg tcgattctta ttgaaatccg agatgaacca tgcggaacgt
    14461 aataatattt tagaaatcag agaaagaatg tatgaaaaag gatggaaaac aggagaagtg
    14521 atcggccagt atctattgac gaatacggat gaaaacaata tcagaactga atattatcat
    14581 gcatggatag aaatgaaaaa ataggaaata tgtgtttaag ataactcgaa aatgatggtt
    14641 gttttagtta taaagaaaaa ttttctggtt cgattacggg tttaatgtgg gggattatta
    14701 gtcgtggata gatagaattt tagtttttta gaggaaatat gtctttaaaa atggaatggt
    14761 tgcaattctt agcgatatat acgatgtaat tgtcctctta aaaaagtatg gaaaaataat
    14821 tgtattgggt gttatgtata attcatggtg aactgagact aatgagaaga aaaattcata
    14881 ttgttatgag tgagattgta tcagatgaga atgataaatt aaataggtgc ataaaagcat
    14941 tacacttaaa atacattgtt gcttacatta ttacaatatg ttaataaggc aaaaataaat
    15001 agatggattg aataaacata ttttttaatc aaatagtccc tactgtgact ttcctcagct
    15061 atcggtgata ctaccgatag catttttttg cttataaaat aattataagt atttagagat
    15121 ttttcttcta acattcttca aacaagaaaa atcacaaagg ttattgttat ttttatatat
    15181 tctttttaag aatgattgtt tttttataga aattttaaaa aataaaacac gttattggtg
    15241 gatagttagt ttgttatttg gtaatattat agaagttgac tggtagtgat ttatttttcg
    15301 tcgttttata aaaatgttat tttttggaaa aattcacaaa tatgtaacga atgtttttct
    15361 ggaatttgct gtgttaaaaa gcagtatgac taatataaat actattgtca tttatgcaat
    15421 tttttatgga tagtaatttt agaggggaat caatgaagga agaatagaag ggaaaaatag
    15481 ttgcaaatac gccagttagc aaaaatagca atacatcttc aagctttaaa attcgagaga
    15541 atcatcttgg tagtggaatc aactacaaca aaaggattaa accaattaat agatagaatt
    15601 accaatggaa aaatagaaaa ggtagttatt ctatacaaaa gttgtttaat tcgcttcggt
    15661 tatgaactta gtgaaaccct ttgcaatata tggtacagaa tatagaaatt attgacatta
    15721 cagaaaatga tgaagattta atacagattg tcactatttg tagatacctt ttacaaggaa
    15781 accgagccaa taaagctaag aagatgatta aggagttgat ggaagacgat acaagttaag
    15841 aaagtgaaac tacgcccaaa taaagagcaa aaagagtaat tggacgtgga aaatgcaaga
    15901 aatgaaccat cgattcggtg gacagtttat tagtaatgac ggtttgagaa catccatgac
    15961 gagaatgaag gaacatccta aatgcgttta gttgaaccaa gtgccaaata atatggccaa
    16021 ataagcgatc agttatcgat gaatcccaag tattctaaac tatatattca ttttgatgct
    16081 aagcattgat atattttttt gggattgatg aaggttaacc aatacaggga ctcactgatg
    16141 aaattctaga gtctgacctt ggtatcaacg atttagtgat tgtttctaat ggaaaagtat
    16201 aaaagtatat aagtacatta acaaaataac tattagggtt acgctccagc ttgtgcaagg
    16261 cactggcagg aggaatcgtg aaatcataat tatggttaag ccacacggac aactaaaagt
    16321 catacaaagc tatcgaatca atcaacagaa gacaatcggc aatcattagc tttgtttaca
    16381 gcttacattg caacgcttga gttgaaccga aaatacgagg gaggtaacgc agccattttg
    16441 agttggggaa tctttttaga aaattatcgg gtaccttgtt gtttagtcaa aattataagg
    16501 gggagtaata gtgaggaaca aatttcataa actgttaagt atgtttctag ttatgcttat
    16561 tgtgatcaat caatttggga acataacggt tctagctgag tctttagcag aacagccaga
    16621 gcgttcgctg acgatcgaag ttgatcaaga ggaggctgct ttgggtgagc agttccagtt
    16681 gcgagtcaga ggaacaccag acctgctaaa tgcattggtc attgataaga gtgaggattt
    16741 gattgaaggg gaagtgaagc aactcaatga ttacgaacgt ctgattactt attcttccca
    16801 gcattcaggt aaatacgaat tgaccgcaag aagcggtgat attcaatcaa atacggtctc
    16861 aatagaaatc agtgtaccag aagaaagcac aagtgagtct tttaatgtac cagaagaaag
    16921 cacaagtgaa tcttttaata taccagaaga aagcttaagt gaatctttta atgaagccag
    16981 atcatctgag ccatctataa cgcaggaatc tcagattgaa agacaagagg agctcttgaa
    17041 ttatgacatc ggcatctctg ctgagcgccc cactttgccg agcaggcata cacaaacgtt
    17101 aaagggatac attcaaggag gcgctggcga tattcttgaa gaatatgggg agatggtcgt
    17161 acgagttcct agagcatcga ttggcttaat tgagttcatt agagtggagg tcgctgatga
    17221 ttcaccttta aagccactgg cattttacca agaaggcaca gacgttgttt ttccatttac
    17281 ctttgattat gaaggattaa atgaagccgc tcagtcgtat acattcagta tcacgtttga
    17341 agttgctttc tttcaatcag gcggtactga gccaaataat gactttcaag tgagtcttac
    17401 ttatgctgag cgagcagcaa acacggaatt ttcaattacg cctattcctt catcaagacc
    17461 cattttttcg aaattatact atggctctag agaaatcgac agccaaggaa atcttgggaa
    17521 tctgctgatt gacgggcaag acgtgaccaa cagtaataaa tttattttgc aggtaaatta
    17581 tgaaggatca cgaaaagacg gtgtagtggt taccgatgta ctaccgcaag gaacaacttt
    17641 ggcaccgaat ccgactacat ggagttccac tggaaataga gaacctattt caggtggggt
    17701 tcggatcgca gaagtaacga atccaggtga agcaaacgtt agaactcatt atgtttccga
    17761 ggaatttgtg gacaaagtat cttttaataa ggaaacgaat acacttgttg tggactttgg
    17821 taatctagca gagggtcaat cgtattttat tgaatatgct cttgaagtga ccgatgtcga
    17881 cttgttcaat gaaggaagac aagaggatgg gttttctcgg aatcgagcga gtatgaccta
    17941 caataatcag gtaatgagcc gagactaccg cttgagaatc cctgaaagta atcttccggc
    18001 aatctcttta agaaaaagtg tggacaaaaa tgttatcaat ctcaatgaaa atgaactaac
    18061 gtatactctg catgtctcga tgttttctgg aacaagaaat acgcttgtta ttgaagatgt
    18121 tttggaagaa ggcatggaat ttgtgtcatt tctatcagat attccctctt tcattgatgc
    18181 cgaagtcaat aatggaatag ttcgtttcag tgcgaataat acgattggtg gtccgacaag
    18241 ttttaccctc tcttttaagg tcaatgtaga ggcgtatgcg gtcggcgatc aaattttaaa
    18301 ctttgcaacg cttgtgaata gtgacaatcg attgaatacg agtactgtaa gaacaaggaa
    18361 aatcgatggg cgtattaaag taattaaaac tgatgagaat ggccaaaggc tggcaggagc
    18421 aaaatttgaa attttatcaa gcgattcaca agtgatacaa gaaggtataa cggcagaaaa
    18481 cggagaattt ttaagtgaac cgcttcctgt aggtagctat caagtccgag aaacagaagc
    18541 accggaaggg tatcttttag atgcgacggt acatgatgtt tttataaatg aatactccat
    18601 agaagaagtg acgttaacca ttgaaaacat ccttgatacg ggtggtgttg aactaattaa
    18661 acatgctgaa ggcagtggtg aagtccttca aggagctgtt tttgagttac agaaccgaga
    18721 aggagaaacg ctacaaaccg gtttaactac aggagaagat ggaaaactgg caattgacgg
    18781 tttagcacc
//
LOCUS       DACCCS010000053        17391 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.054, whole genome shotgun sequence.
ACCESSION   DACCCS010000053 DACCCS010000000
VERSION     DACCCS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.054"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            324..632
                     /gene="rpsJ"
                     /locus_tag="IVK82_002011"
     CDS             324..632
                     /gene="rpsJ"
                     /locus_tag="IVK82_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP6393057.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            663..1292
                     /gene="rplC"
                     /locus_tag="IVK82_002012"
     CDS             663..1292
                     /gene="rplC"
                     /locus_tag="IVK82_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP6393058.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1320..1943
                     /gene="rplD"
                     /locus_tag="IVK82_002013"
     CDS             1320..1943
                     /gene="rplD"
                     /locus_tag="IVK82_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP6393059.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1943..2233
                     /gene="rplW"
                     /locus_tag="IVK82_002014"
     CDS             1943..2233
                     /gene="rplW"
                     /locus_tag="IVK82_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP6393060.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2272..3105
                     /gene="rplB"
                     /locus_tag="IVK82_002015"
     CDS             2272..3105
                     /gene="rplB"
                     /locus_tag="IVK82_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP6393061.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3148..3426
                     /gene="rpsS"
                     /locus_tag="IVK82_002016"
     CDS             3148..3426
                     /gene="rpsS"
                     /locus_tag="IVK82_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP6393062.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3449..3796
                     /gene="rplV"
                     /locus_tag="IVK82_002017"
     CDS             3449..3796
                     /gene="rplV"
                     /locus_tag="IVK82_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP6393063.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3810..4466
                     /gene="rpsC"
                     /locus_tag="IVK82_002018"
     CDS             3810..4466
                     /gene="rpsC"
                     /locus_tag="IVK82_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP6393064.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4469..4903
                     /gene="rplP"
                     /locus_tag="IVK82_002019"
     CDS             4469..4903
                     /gene="rplP"
                     /locus_tag="IVK82_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP6393065.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4893..5081
                     /gene="rpmC"
                     /locus_tag="IVK82_002020"
     CDS             4893..5081
                     /gene="rpmC"
                     /locus_tag="IVK82_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP6393066.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            5106..5372
                     /gene="rpsQ"
                     /locus_tag="IVK82_002021"
     CDS             5106..5372
                     /gene="rpsQ"
                     /locus_tag="IVK82_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP6393067.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5430..5798
                     /gene="rplN"
                     /locus_tag="IVK82_002022"
     CDS             5430..5798
                     /gene="rplN"
                     /locus_tag="IVK82_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP6393068.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5835..6143
                     /gene="rplX"
                     /locus_tag="IVK82_002023"
     CDS             5835..6143
                     /gene="rplX"
                     /locus_tag="IVK82_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP6393069.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6171..6710
                     /gene="rplE"
                     /locus_tag="IVK82_002024"
     CDS             6171..6710
                     /gene="rplE"
                     /locus_tag="IVK82_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP6393070.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6729..6914
                     /locus_tag="IVK82_002025"
     CDS             6729..6914
                     /locus_tag="IVK82_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP6393071.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6950..7348
                     /gene="rpsH"
                     /locus_tag="IVK82_002026"
     CDS             6950..7348
                     /gene="rpsH"
                     /locus_tag="IVK82_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP6393072.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7380..7916
                     /gene="rplF"
                     /locus_tag="IVK82_002027"
     CDS             7380..7916
                     /gene="rplF"
                     /locus_tag="IVK82_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP6393073.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            8077..8433
                     /gene="rplR"
                     /locus_tag="IVK82_002028"
     CDS             8077..8433
                     /gene="rplR"
                     /locus_tag="IVK82_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP6393074.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8454..8954
                     /gene="rpsE"
                     /locus_tag="IVK82_002029"
     CDS             8454..8954
                     /gene="rpsE"
                     /locus_tag="IVK82_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP6393075.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8969..9148
                     /gene="rpmD"
                     /locus_tag="IVK82_002030"
     CDS             8969..9148
                     /gene="rpmD"
                     /locus_tag="IVK82_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP6393076.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9192..9632
                     /gene="rplO"
                     /locus_tag="IVK82_002031"
     CDS             9192..9632
                     /gene="rplO"
                     /locus_tag="IVK82_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP6393077.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9632..10927
                     /gene="secY"
                     /locus_tag="IVK82_002032"
     CDS             9632..10927
                     /gene="secY"
                     /locus_tag="IVK82_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP6393078.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10989..11636
                     /locus_tag="IVK82_002033"
     CDS             10989..11636
                     /locus_tag="IVK82_002033"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP6393079.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11827..12045
                     /gene="infA"
                     /locus_tag="IVK82_002034"
     CDS             11827..12045
                     /gene="infA"
                     /locus_tag="IVK82_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP6393080.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            12078..12194
                     /gene="rpmJ"
                     /locus_tag="IVK82_002035"
     CDS             12078..12194
                     /gene="rpmJ"
                     /locus_tag="IVK82_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP6393081.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12212..12577
                     /gene="rpsM"
                     /locus_tag="IVK82_002036"
     CDS             12212..12577
                     /gene="rpsM"
                     /locus_tag="IVK82_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP6393082.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12605..12994
                     /gene="rpsK"
                     /locus_tag="IVK82_002037"
     CDS             12605..12994
                     /gene="rpsK"
                     /locus_tag="IVK82_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP6393083.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            13074..14012
                     /locus_tag="IVK82_002038"
     CDS             13074..14012
                     /locus_tag="IVK82_002038"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP6393084.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            14040..14420
                     /gene="rplQ"
                     /locus_tag="IVK82_002039"
     CDS             14040..14420
                     /gene="rplQ"
                     /locus_tag="IVK82_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP6393085.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14692..15531
                     /locus_tag="IVK82_002040"
     CDS             14692..15531
                     /locus_tag="IVK82_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6393086.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15507..16376
                     /locus_tag="IVK82_002041"
     CDS             15507..16376
                     /locus_tag="IVK82_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6393087.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16366..17163
                     /locus_tag="IVK82_002042"
     CDS             16366..17163
                     /locus_tag="IVK82_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP6393088.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT         5809 a         3138 c         3815 g         4629 t
ORIGIN      
        1 ttttatttat tgcaggatca ggaaaaaaga caggagtatt ataaatagaa gaaacacgaa
       61 ataaaagagt cagtattttc taaatgaaac tttatgaaaa caaaatgttt tttacaagaa
      121 aaagccttgc tttgtgagtt tatatatatt ataatgacaa aggtgcgttt actttagtga
      181 attgcacaga cgtaatcaga atgatttcgt cggctaagaa acgagaggtt gcgacacgcc
      241 cggacgcttt gccacgaacg agcgtgacgg aaattttcgt ggagctatgt ctacttttca
      301 aaataggcga aggagggaac aagatggcaa aacaaaaaat tcgtatccgt ttaaaagcgt
      361 atgaacaccg tattttagat caatcagcgg ataaaatcgt ggaaactgca aaaagaactg
      421 gagctagcgt gtctggtcca attccattac caactgaacg cagtctttac acaatcatcc
      481 gtgcgactca taaatacaaa gattcacgcg aacaattcga aatgcgtaca cacaaacgtc
      541 ttatcgacat tgtgaaccca acaccaaaaa ctgttgatgc tttgatgaag ctagacttac
      601 catctggtgt aaacatcgaa atcaaattat aaaactaaaa caaaaataat ggaggtgtac
      661 tcatgaccaa aggaatctta gggaaaaaag tgggaatgac acaaatcttc actgaatctg
      721 gtgaattaat tccagtaact gttgtagaag ctactccaaa cgtagtacta caagtgaaaa
      781 caatggaaac ggatggctac gaagctattc aagttggtta ccaagatatg cgtgaagttt
      841 tatcaaacaa acctgcgaaa ggtcatgttg caaaagcaaa cacggctcct aagcgcttca
      901 ttcgtgaatt caaaaatgtt gagctaggag aatatgaagt aggaaaagaa attacagtag
      961 atgttttcca agcaggagac attattgatg taacaggtac tacgaaaggt aaaggattcc
     1021 aaggggttat caaacgccac ggacaaagcc gcggaccaat ggctcacggt tctcgttacc
     1081 atcgtcgtcc tgggtcaatg ggtccagttg cacctaaccg tgtatttaaa aacaaacgtc
     1141 ttgctggccg tatgggtggc aaccgcgtaa ctatccaaaa cttggaagtt gtacgtgtcg
     1201 atgcagaaag aaatgttatc ttgatcaaag gtaatgttcc tggagctaaa aaatctttaa
     1261 ttactatcaa atcagctgtg aaagctaaat aattgtagga agaaaggagg aactaaggaa
     1321 tgccgaatgt agcattattc aaacaagatg gaagccaaaa cggtgaaatc acacttaacg
     1381 aagaaatctt cggaattgaa cctaatgaaa gtgttgttta cgatgcaatc gtaatgcaac
     1441 gtgcgtcatt aagacaagga acacacgcgg ttaaaaaccg tagcgctgtt cgcggcggcg
     1501 gacgtaaacc atggcgtcaa aaaggaacag gtcgtgcacg tcaaggatct atccgctcac
     1561 cacaatggcg tggaggtggc gtagtctttg gaccaacacc acgttcttac agctacaaac
     1621 ttcctaaaaa agttcgtcgt ttagcaatga aatctgtatt gtcagaaaaa gttgctgaaa
     1681 acaacttggt agcaatcgaa ggcttgaact tcgacgcacc aaaaacaaaa gaattcaaac
     1741 aagttcttgc taacttatct atcgactcta aagtattggt tgttctagaa aaaggtaacg
     1801 actttgctgc tttatcagca cgtaacttac caaacgtttc tgtagtaact tctgataatg
     1861 tgagtgtgct agacgttgta tcaaatacaa aagtgttagc aacacaaact gctcttactc
     1921 aaattgagga ggtgcttgca taatgaactt actagacgta atcaaacgcc cagtgatcac
     1981 tgaaaaatca atgcttgcca tggatgacaa gaaatacact tttgaagtgg acactcgcgc
     2041 aaacaaaact ttagtaaaac aagcggtaga agctgctttt gacgttaaag tcaaaaacgt
     2101 gaacatcgtg aacgtacgtg cgaaattcaa acgtatgggc aaatatgcag gatatacaaa
     2161 aaaacgtcgc aaagcgatcg ttacattgac tgaagattca aaagaaatcc aattattcga
     2221 agctgctgaa taagcactaa tagataactc attaaattag gagggaaaaa cgtggcgatt
     2281 aaaaagtaca aacctacctc aaatggtcgt cgtaacatga caggttctga ttttgctgaa
     2341 atcacaacat caacacctga aaaatcattg ttacaaccat tgaaaaacca tgctggacgt
     2401 aacaacaacg gccgcatcac tgtacgtcat caaggtggcg gacacaaacg ccaatatcgc
     2461 gtgatcgact tcaaacgtaa caaagataac gttgttgcaa ctgttcaaac gatcgaatat
     2521 gatccaaacc gttcagctaa catcgcgtta gtacactatg aagatggagt gaaagcttac
     2581 atcttagcac ctaaaggact agaagtgggt atgaaacttg tttcaggtcc agaagcagat
     2641 atcaaagtcg gaaatgctct tccattagaa aacattccag ttggtactgt tatccacaac
     2701 attgaaatga aacctggtaa aggtggacaa ttgatccgtt ctgctggtac aagtgctcaa
     2761 gtacttggta aagaaggcaa atacgtattg atccgcttga actctggtga agttcgtatg
     2821 atcttagctg catgccgtgc tacaatcggt tctgtaggta acgaacaaca cgaattaatc
     2881 aacatcggta aagctggacg ctctcgttgg atgcgtaaac gcccaactgt acgtggtagc
     2941 gtaatgaacc ctaatgatca cccacacggt ggtggtgaag gtaaagctcc tatcggacgt
     3001 aaagcaccag tttctccttg gggtcaacca gctcttggtt acaaaactcg taacaaaaaa
     3061 gctaaatcag acaaacttat tgttcgtcgt cgcaaaaata aataattact ttgaccatgt
     3121 gtcgcacatt ttgagaggag gttcaccatg ggtcgtagtt taaagaaagg accttttgtc
     3181 gatgagcatt taatgaaaaa agttgaagct caagcagggg ttgaaaagaa aaaagttatc
     3241 aaaacttggt cacgccgttc aacagttttc ccaagttttg ttggatatac catcgcagtt
     3301 tatgatggac gtaaacatgt accagtttac atccaagaag acatggtagg acacaaatta
     3361 ggtgaatttg cacctacaag aacttatcgt ggacatgctg ctgacgataa gaaaacgaaa
     3421 cgctaatttg aggggaggat aaatccaaat ggcagaacaa atcacatcag ctaaagcaac
     3481 tgcaaaaaca gttcgcactt cacctcgaaa agcccgttta gtaattgacc ttatcagagg
     3541 taaaagtgtc gcagatgcaa tttcaatctt gaaattcaca ccaaacaaat ctgctggaat
     3601 cattgaaaaa gtattgatgt cagcagttgc gaatgcagaa aataactttg acttagatgt
     3661 tgaaaacttg gtagtaagcg aagcttttgt taacgaagga cctacaatga aacgtttccg
     3721 tcctcgtgca aaaggatcag cttctccaat caacaaacgt acaagtcata tcacggtagt
     3781 cgtatcagaa aaataaggag ggataatcag tgggtcaaaa agtacatcca attggaatgc
     3841 gtgtaggcat catccgcgac tgggatgcta aatggtatgc tgaaaaagag tatgctgagt
     3901 tcttacacga agatttaaga atccgtaaat ttatttcaac taaacttgct gatgctgctg
     3961 tatcaacagt tgaaatcgaa cgcgcaacag gtcgcgtaaa catttcaatt cacacagcta
     4021 aaccaggtat ggttattggt aaaggcggtt ctgaagtcga aagcctaaga aaagaattaa
     4081 acaaattaac tggtaaaaga atccatatca atatcgtgga aatcaaaaaa ccagatctag
     4141 atgcaaaatt agtaggtgaa ggaatcgcac gtcaattaga aaaccgtgtt gctttccgtc
     4201 gcgctcaaaa acaagccatc caacgctcaa tgcgtgctgg cgctcaaggg atcaaaactc
     4261 aagtatctgg tcgtctaaac ggagcagata tcgctcgttc agaaggatac tcagaaggaa
     4321 ctgttcctct tcatacttta cgtgcggaca ttgattacgc atgggaagaa gcagatacaa
     4381 catacggaaa actaggagtt aaagtgtgga tctatcgtgg agaaattctt ccaactaaaa
     4441 aaaacactga gaaaggaggg aaataatcat gttagtacct aaacgtgtaa aacaccgtcg
     4501 tgaattccgc ggaaaaatgc gcggggaagc taaaggcgga aaagaagtag cattcggtga
     4561 atacggtttg caagctgttg attcacattg gatcacaaac cgccaaatcg aagctgctcg
     4621 tatcgcaatg actcgttaca tgaaacgtgg tgggaaagta tggattaaaa ttttccctca
     4681 caaatcttat actgccaaag caattggggt acgtatgggt tctggtaaag gggcacctga
     4741 aggatgggtt gcaccagtaa aacgtggtaa aatcatgttt gaaatcgcag gcgttcctga
     4801 agaagtagct cgtgaagcgt tacgtctagc ttctcacaaa ttaccaatga aaactaagat
     4861 cgtaaaacgt gaggaaatgg gtggtgaatc gaatgaaggt taaagaaatc agagaattaa
     4921 ccactgccga aatgcttgat caagaaaaac aattaaaaga agaattgttt aatcttagat
     4981 tccaattagc aacaggtcaa ttagaaaaca ctgcacgtat taaagaagta cgtaaatcga
     5041 ttgcacgcat caaaactgtg ttgcgtgaac aagctaagta attatggaag gaggccatta
     5101 agcgtatgac tgaaggaaga aatcaacgca aagtttatca aggtcgcgtg gtttcagaca
     5161 aaatggataa aacaattacc gttgctgtcg aaacaaagaa aaaccaccct atctatggca
     5221 aacgcatgaa ctattctaag aaatacaaag tgcatgatga aaacaacaca gcaaaagttg
     5281 gcgacatcgt gagaattatg gaaactcgtc cattatcagc tacaaaacgt ttccgtttgt
     5341 tggaagtagt cgaagaagca gttattatct aataacacga gattttccta tatagattga
     5401 atgcaaaatc tgaaaggagg atacacaacg tgatccaagc agaaagtcgt ttaaaagtcg
     5461 ctgataactc aggcgcacgt gaaattttga cgatcaaagt ccttggtgga tctggtcgta
     5521 aaactgctaa catcggtgat gtgatcgttg ctacggttaa acaagcaacg ccaggtgggg
     5581 ttgtcaaaaa aggtgacgtc gttaaagccg ttatcgttcg aacaaaatca ggagctcgtc
     5641 gtacagacgg ttcatatatt aaatttgatg aaaatgctgc agtgattatt cgtgatgata
     5701 aaagccctcg tggaacacgt atctttgggc cagttgcacg tgaactacgt gaaaacaact
     5761 tcatgaagat cgtttcacta gcaccagaag ttttataatc ttgagacact tatcaaagga
     5821 ggtgcgaaac agttatgttt gttaaaaaag gcgataaagt taaagttatc actggaaaag
     5881 acaaaaacaa agaaggcgtt gtactagcag cgttcccgaa aaaagataaa gttattgtcg
     5941 aaggtgtaaa tgtcgttaaa aaacaccaaa aaccttctca agctgctcct caaggtggaa
     6001 ttgttgagat ggaagcgcca attcatgttt ctaacgtgat ggtggttgac tctactggtg
     6061 tagctggccg agttggctac aaagaagtag acggcaaaaa agtccgtgtt tctaaaaaaa
     6121 ccggtgaagt tttagataaa taaaagttta aggaaggagg gtcttactga atgaaccgcc
     6181 taaaagaaaa atatcttaaa gaagtaactc catcattgat ggaaaaattt aactatagct
     6241 ctgttatgca aacacctaaa gttgaaaaaa tcgtcatcaa catgggtgtt ggtgatgcgg
     6301 tatcaaacgc taaaaactta gataaagcag ttgaagaatt aactttgatc actggtcaaa
     6361 aaccaatgat tacaaaagct aaaaaatcaa tcgctggatt ccgtttacgt gaaggaatgc
     6421 caattggtgc aaaagttaca ttgcgcggag aaagaatgta cgaattctta gacaaattgg
     6481 tttctgtttc actaccacgt gtacgtgact tccatggtgt aagtaaaaaa gcctttgacg
     6541 gtcgtggaaa ctatacttta ggtattaaag aacaattgat cttcccagaa gtagattatg
     6601 atttagtaga taaagtacgt ggtatggaca tcgttattgt aacaacagca aactctgatg
     6661 aagaatctcg tgagttgttg acacaattag gcatgccatt ccaaaaataa ttaaaggagg
     6721 cgaactacgt ggctaaaaaa tcaatgattg ctaaaaacaa acgtcctgca aaattttcaa
     6781 cacaagctta tactcgttgc gaacgttgcg gacgtccaca ttcagtttat cgtaagttcc
     6841 atctttgccg tatttgcttc cgcgaacttg cctataaagg tcaaattccc ggcgtgaaga
     6901 aagctagctg gtaaaaagta aattcgcata agaaggaggt aaaagatcaa tggtcatgac
     6961 agatccaatt gcagattttc taactcgtat ccgtaacgca aacatggtta aacatgagtc
     7021 tttagaagta cctgcatcaa aaattaaacg tgacatcgct gaaatcttga aacgtgaagg
     7081 tttcgttcgt gatgttgaat acatcgaaga tgacaaacaa ggcgtgatcc gtgttttcct
     7141 aaaatttggt aaaaacggcg aacgtgttat cacaaactta aaacgtatct ctaaaccagg
     7201 tttgcgtgct tatgtcaaag ctgacgaagt accaaaagta ttgaacggtt taggtatcgc
     7261 aatcatttcg acttctgaag gtgttgtcac tgataaagaa gctcgtgaaa aaaatatcgg
     7321 cggcgaagtt atcgcttacg tatggtaatc aagtagaatc tacaaggagg tgtctttaag
     7381 tgagccgtat cggaaataaa gtcgttgtta tccctgaagg agtaacagtt actcaagacg
     7441 gaaacaacgt tacagttaaa ggaccaaaag gtgaattgac tcgtactttt tcagcagata
     7501 tcaaaatgaa tatcgaagga aacgaagtaa ctttcactcg tccaaacgac agtaaagaaa
     7561 tgaaaacaat tcatggaaca actcgtgcga acttcaacaa catggttgta ggtgtcagcg
     7621 aaggtttcca aaaagcgtta gaactaatcg gggttgggta ccgtgcacaa ttacaaggat
     7681 caaaacttgt attgaacgtt ggttactcac atccagtaga aatcacacca ccagctggag
     7741 taactgttga agtaccagct aacacacaag taattgttaa aggtgcgaac aaagaagaag
     7801 ttggcgaatt agctgcaaac attcgtggaa ctcgtcctcc agaaccttat aaaggtaaag
     7861 gtattcgtta tgttggcgaa tatgtacgcc gtaaagaagg taaaactggt aaataatcgt
     7921 ttagaaaaat tgcatagaag ccagcagcag gaaaaaggca tttggctgaa gtttaccttc
     7981 aaaaaaatac atttttactt gtgccgcagc ttctattttt cgaaacgtat ttactgcttg
     8041 aaatagtagt acaataaaaa taaagaggtg acaattgtga ttacaaaacc agataagaac
     8101 aaaacacgtc aaaagagaca tcgtcgtgta cgtaacaata tctctggtac tgctgagcgc
     8161 ccacgcttga acgtttttcg ttctaacaaa aacatctacg ctcaaattat tgatgacgta
     8221 gcgggtgtaa cgctagcaag tgcctctacc ttggataaag aaatttcagg tggaacaaaa
     8281 acagaagcag cacaagctgt cggtaaacta gttgctgaac gtgcagctga aaaaggcatt
     8341 aaagtagtag tctttgaccg tggtggatac ctttaccatg gccgtgtgca agctttagct
     8401 gaagctgctc gcgaaaatgg actagaattt tagaagaagg aggaatacca ttcatggttt
     8461 atatcgatcc aaaacatttg gaattagaag accgcgttgt tgcaattaac cgtgtaacaa
     8521 aagttgttaa aggtggacgt cgtctacgtt ttgctgcttt agttgttgtc ggtgacaaaa
     8581 acggacacgt aggatttggt acaggtaaag ctcaagaagt tcctgaagca atccgtaaag
     8641 caattgaaga tgcgaagaaa aacttagttg aagtacctat ggttggttca acaatcccac
     8701 acgaagtaat cggtgctttt agtggtggta gaatcctaat gaaacctgct gtagaaggtt
     8761 ctggggttgc tgctggtgga ccagttcgtg ccgtattaga attagctggg gtagcagata
     8821 tcacttctaa atctttaggc tcaaatacac caatcaacgt tgttcgcgca acagttgaag
     8881 gtttgaaaca attgaaacgt gcagaagaag ttgcagaact tcgcggcaaa tcagttgaag
     8941 aattaatcgg ataaggagga caacaataat ggctgaatta aaagttactt taaaacgcag
     9001 tattatcgga cgtcctcaaa accaacgcga tacagttaaa gcgttaggtc ttacaaagat
     9061 taacagttca gtggttaagc cttctaatga agcaattaaa ggcatgatca atactgtttc
     9121 tcacctagtg gacgttgaag aaatttaatt tagaaaaatc ataactagaa cattaaggag
     9181 gtgccaaacc tatgaaactt catgaattaa aacctgctga aggttcacgc caagtacgta
     9241 accgtgttgg tcgtggtact tcatctggta acggaaaaac tgccggacgc ggacaaaaag
     9301 gacaaaaagc tcgttcaggc ggcggtgtgc gtttaggatt tgaaggggga caaacaccat
     9361 tgttccgtcg tttaccaaaa cgaggattca caaacgtgaa ccgtaaagac tatgcagtca
     9421 ttaatcttga tgtattgaac cgctttgaag acggagctga agtaactcct gttgctttag
     9481 ttgaagctgg aatcgtgaaa aacgaaaaag ctggaatcaa agtattagct aatggggaac
     9541 taaacaagaa attaactgtg aaagcagcaa aattctctaa agcagctcaa gaagcaattg
     9601 aagcggctgg tggatcaatc gaggtgatct aatgtttaga ctattaaaag atgcttttaa
     9661 agtaaaagac attagatcga aaatcctatt tactgtattt gtgttgtttg tatttcgcct
     9721 aggtgcacat attactgtac caggcgtaaa tgctgcttca ttgcaaaacc tttcttcttt
     9781 gccatttttc aacatgttga acttggtaag tggtagtgcg atgcaaaact tttccatttt
     9841 ctcaatgggg gtttcaccat acattactgc ttctatcgtc attcagcttc ttcaaatgga
     9901 catcgtgcct cgatttgttg aatggtcgaa acaaggggaa gttggtcgta aaaaactgaa
     9961 tcaagcgact cgatatctga cattagtatt ggcatttatc caatctgtcg gtatcactgc
    10021 tggattcaat caatacgctc aattaggttt cgtgaataat cctaacgtag caacttacgt
    10081 aacgatcggt ttgattctaa cagccggtac gatgtttgtt acttggctag gagaacagat
    10141 cacagaaaaa ggtatcggaa atggcgtatc aatgatcatt tttgcaggta tcatttctag
    10201 attgcctgaa agtatcagag aaatttatga agattacttt ataaatattg atccttcaga
    10261 catatggaaa tctgctattt ttgtggccat tttactagta gcggtcttgt tgatcgtgac
    10321 attcgtaaca tttttccaac aagctgaacg caagatcccg atccaatata cgaaacgcgt
    10381 agctggcgcg cctacaagca gctatctgcc gttgaaagta aatgcagctg gagttattcc
    10441 agttatcttt gctagttcgt ttattgctac gccaaatgct gttttacaag cattgagcgg
    10501 tagattcggt tctgaaaact ggtacaaagt gatgatggag atctttaact ataatacagc
    10561 acctggtgct acgatttata cgattctgat cgttgccttc actttcttct acgcctttgt
    10621 tcaagtaaac cctgagaaat tagcggaaaa cttgcaaaag caaggaagtt atataccaag
    10681 cgtacgccct ggtaagggga ctgaagagta tgtctcaaga ttactgatgc gtttaagtac
    10741 agttggtgca gtcttccttg gtttagtggc attgcttcca atcattgctc aaatggtttg
    10801 ggatcttcca caatcaatcg gattaggcgg tacaagctta ctgatcgtta tcggtgtagc
    10861 tttagaaact gcgaaacagt tagaaggctt aatgctgaaa cgtaaatatg ttggattcat
    10921 tgagtaagaa agcgtgttga ggtttcccct caacacacgt tctcacacat ttattaggag
    10981 ggaaaacaat gaacctcatt ttaatggggc tgccaggcgc aggtaaagga acacaagctg
    11041 aaaaaatcat tgatgcttat ggtattccgc acatctcaac aggagatatg ttccgcgcag
    11101 cgatgcaaaa cgaaacagct cttggcctgg aagcaaagtc ttatatggat aaaggtgcat
    11161 tagttcctga tgaagtaaca aatggaatcg tgaaagagcg actagctgaa ccagatacag
    11221 agaaaggctt cttattagat ggctttccac gtacgctaga tcaagcaaaa gctctggacg
    11281 caatgctaaa agatttgaat aaaaaaattg atgctgtcat cgatatccat gttggtgaag
    11341 aaatcttggt cgaacgtcta gccggccgat ttatctgcag caattgtgga gcaacttacc
    11401 ataaaatttt caaccctaca aaagttgaag acacatgtga tcgttgcggt gggcatgaat
    11461 tctatcaaag agaagatgat aaacctgaga cggttaaaaa tcgtctggct atcaacatca
    11521 aaaatagtga accaatcttg gcttattata aagaacaagg tttgctaaac actattgatg
    11581 gtgctcgtga aatcgatgct gtatttgcag atgtacaaaa aatcattgaa aaatagcacg
    11641 attgcccgta gccgacttgc attatgcagt tactcttgtc aagttcaata aattatgata
    11701 gaatatgtca gtccgacttc tagagaatcc tctagatgat ccattttgag aattttgagg
    11761 tgggaactgc agtttccgcc attttatcgt gtgaaaatgc gaaacaagta caaggaggta
    11821 ctgtgcgtgg caaaagaaga tatgattgaa gtcgaaggta cagtcgtcga aactttgccg
    11881 aatgcaatgt ttaaagttga actagaaaac ggacaccaag ttcttgctac tgtttcaggt
    11941 aaaattcgta tgcattacat ccgtatctta cctggtgaca aagtaacggt tgaattgtct
    12001 ccatacgatc ttaaccgtgg ccgtattact tatcgcttta aataattgta ctccgtaaaa
    12061 ctacaggagg tattatcatg aaagtaagac catcagtaaa accaatgtgc gaacattgta
    12121 aagtaattcg tcgtaaagga cgcgttatgg tgatttgccc agcaaatcca aaacataaac
    12181 aacgtcaagg ataattggag gtgctataaa tatggctcgt attgcaggag tagatattcc
    12241 tcgtgataaa cgtgtagtag tttcacttac ttatatctat ggtattggta acactactgc
    12301 taaaaagatc ttagctgatg ctggtgtatc tgaagatgtt cgcgttcgtg aattaacgaa
    12361 tgaacaaaca gatgctatcc gtgcagaagt agataaatta aaagttgaag gggatcttcg
    12421 tcgtgaagtg aacttgaaca tcaaacgttt gatggaaatc ggttcttacc gtggaatccg
    12481 tcatcgccgt ggattgcctg ttcgtggaca aaacacgaaa aacaatgcac gtactcgtaa
    12541 aggcccagct cgcgctatcg ctggcaagaa aaaataattc ttaagtgaag gaggttaaaa
    12601 cttcatggca gcaaaaaaag tgaatcgtaa acgccgtgtg aagaagaata tagaagcagg
    12661 tattgcacat atccattcta cattcaataa cactatcatc atgatcactg atactcacgg
    12721 aaatgcatta gcgtggtcat cagctggttc attaggtttc aaaggaagca aaaaatcaac
    12781 tccttttgcc gctcaaatgg cagcagaaac tgctacaaaa gcagcaatgg aacacggatt
    12841 aaagactgtt gaagtaacag ttaaaggacc tggttctgga cgtgaagcag caattcgttc
    12901 attacaagct acaggtttag aagtgactgc aattcgtgac gtgactccag ttcctcataa
    12961 tggtgcccgc cctccaaaac gccgtcgtgt ttaatgagtg ccgctcattt tgagttttat
    13021 cagtaacgtc attgaacaag acacttttcg ttttgaaagg ggtaaagata agaatgattg
    13081 agttcgaaaa accaagaatc gcaaaaattg atgaagaaaa agattatggc aagttcatcg
    13141 ttgaacctct tgaaagaggg tatgggacta ctttagggaa ttccctacgt cgtattttat
    13201 tatcttcttt gccaggtgct gccatcacta atattcaaat cgatggtgta ctgcatgaat
    13261 tctctaccat caaaggtgtt agagaagacg tcactcaaat catcttgaac attaaaggct
    13321 tagcgctgaa aatgtatggc caagaagaaa aaacccttga aatcgatatt accggacctg
    13381 ctacagtaac tgctggcgat attatcgttg atagtgatgt agaaattctt aacaaagata
    13441 tgtacatctg tagtgtctct gaaggagcta cgttccatgc tcgcttaaca gtgaaacctg
    13501 gacgtggtta tgttcaagca gatgaaaata aaaaagaaga tatgccaatt ggcgttcttc
    13561 cagtcgattc aatctacaca cctgttcgtc gtgtgaacta tcaagttgag aacacacgtg
    13621 ttggacaccg tgaagatttt gataaattga cgatggagat ttggacagat ggttcaattg
    13681 aaccgttgga agcaatgagt ttgtctgcga aaatcatgac tgaacatttg gatatttttg
    13741 tgaacttaac tgatgaagca aaaaatgctg aaatcatggt cgaaaaagaa gaaacacaaa
    13801 aagaaaaaat gttggaaatg acgatcgaag agttagactt gtctgttcgc tcttacaatt
    13861 gtttgaaacg tgcaggaatc aatactgtac aagaacttac aaataaatct gagcctgaaa
    13921 tgatcaaagt ccgtaactta ggacgtaaat cattagaaga agttaaagca aaattgcacg
    13981 acttaggttt aggtttacgt aaagaagatt aaaacctttt acgaaggagg gaaaccaacg
    14041 tgagttatcg taaactagga cgcacatcta gccaacgtaa agcgatgttg cgtgatttaa
    14101 caactgattt attaatcaac gaacgtattg tgactactga agctcgtgcg aaagaagttc
    14161 gctcaactgc agaaaaaatg atcactttag gtaaacgcgg agatttgcat gcacgtcgtc
    14221 aagctgcggc tttcgtacgt aatgaagttg ccagcgtgcg tgaagaaaat gaggacatcg
    14281 tcattgaatc agctttacaa aaactgttca acgatattgc acctcgttac gcagaacgtc
    14341 aaggcggata cacacgcatt ttgaagacag aaccaagacg cggtgatggt gcaccaatgg
    14401 ttgtcttaga acttgtctaa aatcacacta ataacatcac tttatagatg attcttttag
    14461 agtgttatga tgttggaaga tatttttccg agtctagctc agcgctgctc ctcaagcgaa
    14521 tatcgcttgg ggagtaagca ctcatcataa agtgtttttt tataccttca attacctgtc
    14581 atgacagata tgtttgaagg cataaaaaaa cattttaagc gagaaataat tgaaaatttt
    14641 gtcttttttt gatagtgtta gtaggaactt taggatgaag aaggagtaat catggagcca
    14701 attattgaat tagaaaagat aaattataaa taccagccag atgatcttcg ccccgcattg
    14761 aaagatgtct cctttacaat tgataaaggt gagtggatcg ctattattgg acacaatggt
    14821 tcaggaaaat cgacacttgc caaaacgatc aatggattgc ttctgccaga atcagggatc
    14881 gtgaaagtgg gaaatcaaat attggatgaa gaaaatattt ggacgatccg acagatggtt
    14941 ggaatggttt ttcaaaatcc agacaatcag tttgttggtt cgacagtaga agatgacgtt
    15001 gcttttggtc ttgaaaatca agggattccc agagaagaaa tgctagtgcg tgtgaaagat
    15061 gcacttgaga aagttagaat ggctgagttt gcttcacgcg agcctgctcg tttatcagga
    15121 gggcaaaaac aacgtgttgc cattgccggc gtggttgcct tacgacctga tatcattatt
    15181 ttagatgaag caaccagtat gctagatcca gaaggtcgtg aagaagtgat ctctacgatc
    15241 aagaagatca aagaagaaag tcaattgacc gttatatcta tcactcatga tattgatgaa
    15301 gcagctaatg ccaatcggat actagtgatg agacaaggcg aacttgtccg tgaaggtaca
    15361 ccaaaagaaa ttttttctgc tggtcctgaa ctgatcgact taggtctaga tctaccattt
    15421 cctgaaaaac tgaagagcgc actgaaagaa cgtggggttg atgtgcccag tgaatatatg
    15481 acagaagaaa ggatggtgga ttggttatgg acatccgttt tgaacaagta gacttcactt
    15541 atcagccgaa tacaccattt gaacaaagag ccttatttga catcaatatg acgatcaaag
    15601 aaaacagtta tactgcatta gtagggcata ccggaagcgg gaaatcaacc ttacttcagc
    15661 atttgaatgc gcttgttaag ccaacaagcg gtacagtcca tattggagaa cgagatattc
    15721 agccggatac ggataataag aatttaaagc ccatccgaaa aaaagttggc atcgtctttc
    15781 agtttccaga agcacagcta ttcgaagaaa cggtagcaaa agacattgct ttcggtccta
    15841 aaaactttgg tgtcagcgaa gaagaagcac tagtcctagc aaaagaaaca ttagaacaag
    15901 ttgggctgga tgaaagctat ttggaacgtt cgccatttga actttcagga gggcaaatgc
    15961 gtagagtagc aatcgctggt gtgcttgcca tgaggccgga agtacttgta ttggatgaac
    16021 caacggcagg acttgatccc cagggcagaa aagaaatgat ggagatgttt tggcgtcttc
    16081 ataaggaaca gcagatcact attgtattag ttacgcattt aatggatgat gtagccaatt
    16141 ttgcagatta tgtctatgtg ttggaaaagg gacgaatcgt taatagcggg gagccgcaag
    16201 aggtctttca aaacattgaa tggctaaaag aaaaacaact aggcgtacct actgccaccg
    16261 agtttgcaga agaattaatg gctaaaggga tgaactttgc taccttgcct ttgacagctg
    16321 aagaattagc agacgctatc gtccattatg ctggaggaaa tgcccatgat gaataaattg
    16381 atatttggtc gttatatgcc tggagattcc ttgatccatc gattggatcc ccgagctaaa
    16441 ttattagcca gtttttattt tatcggaatc atttttttag caaataattg ggaaacgtac
    16501 cttttgttgg catttttcac attactttct gtcttcttgt caaaagtaga tttgaaattt
    16561 tttatccgag gcattcgccc attgatctgg ctaatcctct tcactgtagc tttgcaggtc
    16621 ttgtttacaa gtggcggaga aacttattgg agttggggtg ttttccatat cactgaattt
    16681 ggtgtgatga acggcctctt tattttctgt cgattcgtgt tgatcatttt tatgtcgact
    16741 ctactcacgc ttactacacc gcctttggaa ttatcggatg cgattgagta cttgcttcgt
    16801 ccattgaaag ccgtacggtt cccagtccac gaaatttcat tgatgctgtc gattgctctt
    16861 cgttttgtgc ctacattgat ggacgaaaca gaaaagatca tgaacgctca gcgcgctcgc
    16921 ggtgttgatt ttggtgaagg aaacctagtt cagaaaatga aagcagtcat tcctttgctg
    16981 attcctttat ttgtcagcag cttcaaccgt gcggaagatc ttgcgactgc tatggaagca
    17041 agaggctaca aaggtgggga aggaagaacg aaatatcgtg tattgcattg gcataataga
    17101 gacacgatgg ttatcattgc ttttgtatta ttaactgtgc tactgatctt tttaagagga
    17161 taaacgttca gtgggccgct atctattctt ttcttgctga tctgtttgca agaaaggatg
    17221 caagcagtgt atgacaagaa atattagggt ctgtggcaga ctaaggtcac aggcctcttt
    17281 cttttatctg aaaagaaaga acgaaaaaat tttatagaaa aatcattatt ccagataacc
    17341 ttttaggaac aaaggagaga aaaacatggt acgttacaaa gcaatcattg c
//
LOCUS       DACCCS010000054        17386 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.055, whole genome shotgun sequence.
ACCESSION   DACCCS010000054 DACCCS010000000
VERSION     DACCCS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.055"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(289..768)
                     /gene="rlmH"
                     /locus_tag="IVK82_002043"
     CDS             complement(289..768)
                     /gene="rlmH"
                     /locus_tag="IVK82_002043"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP6393089.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1196..2485
                     /locus_tag="IVK82_002044"
     CDS             1196..2485
                     /locus_tag="IVK82_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP6393090.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2524..3129)
                     /locus_tag="IVK82_002045"
     CDS             complement(2524..3129)
                     /locus_tag="IVK82_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP6393091.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(3334..3798)
                     /locus_tag="IVK82_002046"
     CDS             complement(3334..3798)
                     /locus_tag="IVK82_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6393092.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(3820..4140)
                     /locus_tag="IVK82_002047"
     CDS             complement(3820..4140)
                     /locus_tag="IVK82_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP6393093.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(4284..5363)
                     /gene="pepA"
                     /locus_tag="IVK82_002048"
     CDS             complement(4284..5363)
                     /gene="pepA"
                     /locus_tag="IVK82_002048"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP6393094.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5563..5913
                     /locus_tag="IVK82_002049"
     CDS             5563..5913
                     /locus_tag="IVK82_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6393095.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            6136..7083
                     /locus_tag="IVK82_002050"
     CDS             6136..7083
                     /locus_tag="IVK82_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6393096.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(7140..8558)
                     /gene="pepV"
                     /locus_tag="IVK82_002051"
     CDS             complement(7140..8558)
                     /gene="pepV"
                     /locus_tag="IVK82_002051"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP6393097.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            8707..9552
                     /locus_tag="IVK82_002052"
     CDS             8707..9552
                     /locus_tag="IVK82_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP6393098.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            9556..9996
                     /locus_tag="IVK82_002053"
     CDS             9556..9996
                     /locus_tag="IVK82_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP6393099.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            10046..10240
                     /locus_tag="IVK82_002054"
     CDS             10046..10240
                     /locus_tag="IVK82_002054"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393100.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            10376..11062
                     /locus_tag="IVK82_002055"
     CDS             10376..11062
                     /locus_tag="IVK82_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6393101.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(11470..12159)
                     /locus_tag="IVK82_002056"
     CDS             complement(11470..12159)
                     /locus_tag="IVK82_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393102.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(12134..14263)
                     /locus_tag="IVK82_002057"
     CDS             complement(12134..14263)
                     /locus_tag="IVK82_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP6393103.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(14311..15648)
                     /locus_tag="IVK82_002058"
     CDS             complement(14311..15648)
                     /locus_tag="IVK82_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP6393104.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(15900..16259)
                     /gene="rplT"
                     /locus_tag="IVK82_002059"
     CDS             complement(15900..16259)
                     /gene="rplT"
                     /locus_tag="IVK82_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP6393105.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(16312..16512)
                     /gene="rpmI"
                     /locus_tag="IVK82_002060"
     CDS             complement(16312..16512)
                     /gene="rpmI"
                     /locus_tag="IVK82_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP6393106.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(16584..17108)
                     /gene="infC"
                     /locus_tag="IVK82_002061"
     CDS             complement(16584..17108)
                     /gene="infC"
                     /locus_tag="IVK82_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP6393107.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
BASE COUNT         5085 a         3573 c         3224 g         5504 t
ORIGIN      
        1 atctagcaaa acttaaattt taaataaatc gttatttatt tttactctca cttaaaaaag
       61 cactaaaatt ataagagagg gaggtaatgg tctgaacacc aagaattgtt ccaacatcga
      121 attgcttaac taatgagttc aaagcttaat tatgtggctc ttatatttac cgagcgtgga
      181 ttgcgatctc tttagcgaaa caaacatctt tcataaaaaa aacagccata tccgctttct
      241 tggatatgac tatttactac tactttacta tctttcattc ttcttgtctc acttatgata
      301 aggcgccccc gcattgatcc ggaaggcacg gtagatttgc tcgacaagga tcagacgcat
      361 caattgatgt ggatacgtca tttttccaaa agagatctga gcattgcttc ttttcatgac
      421 ggcttcgctg agtcctaaag agccgccgat aacaaaaaca aggtggcttt ttccttggat
      481 acctaattgg tcgatttgtt tagcaaaaga ttcacttgta ggatttttcc cttcaattgc
      541 taaggcatag acgtattctt gttctttgat tttcgctaag atccgttccc cttctttttc
      601 ttttacttgt ctcatttgcg cttcgcttaa attctccggg gctttttcgt caggaacttc
      661 gatcaattcg atttttgcat aggcattcag ccgtttgaca tactcattga taccttggat
      721 caggtatttt tcttttaatt ttcctactga gataattttg atattcatgt tttctctcct
      781 ttggtcttca tgctcacttt gtttattata atcaattttt tcgtctatat gtttattttg
      841 gctatttttg ttaaaaagat ttccccaaca gttatgcaca acatccacag gtttatccac
      901 agaaaacagt gttattttac cttatttcat cacattcatt aggcaaacat acgttttata
      961 agaatttatc cactttatac acaggctgtg gatatgtttt atcccttatt tttccaatcc
     1021 tatccgcgta gttttacaca gtatctgtgg aaaaagaaaa tcaaatattt tcatctgaaa
     1081 acaatgattt ttcctaaagt ttttcataaa aagttcacag tctcaaagta gactaaagtc
     1141 aacaaataag actatactaa cagtagataa atgaatgtaa ttggaggagg aacacatgga
     1201 tagaaaaaat gtgacaccaa agatgaaaaa aagtaaaaat aatagcctct ggcgtaagtt
     1261 aggtctgggg cttgttggag ggatcgttgg aggactactg acagctggca ttttttatgc
     1321 agcgatgggc actggcaaca atagttctac tgctacaagc ggcaatcaga attctgccgg
     1381 tgaaacagtt gtggaaaatg tcaaagtcaa tgtagattct gatatcacca aagcggtaga
     1441 taaagttcaa gacgctgtcg tttctgttat caacttacaa agtcaaaacc aatcaagcgg
     1501 ttttggcgga ctattcgggc agcaagaaga aagtagcagc tcagatgata gtaatttgga
     1561 agcttatagt gaaggaagcg gcgtgatcta caaaaaagac gggaatactg cttatgtagt
     1621 cacgaataat cacgtagtag atggccagca aggtcttgaa gtcttgatga aagacggtac
     1681 gaaggtaaaa gctgaattag ttggaacaga tgcttattca gatttagcgg tgttgaagat
     1741 caattcagac aaagtcgaaa cagttgcctc ttttggtgac tcaagtgctc tgaaggttgg
     1801 tgagcctgcg attgcgattg gttccccact aggatctgaa tatgcaaatt ctgtcacttc
     1861 aggaatcatt tcttccttga accgacaagt gacaagtacg aatgaatcga acgaaacagt
     1921 caatatcaat gcgatccaaa cagatgcagc aatcaaccct ggaaattctg gtggtccttt
     1981 agttaatatc gaaggacaag ttatcggtat caactcaagt aagatcgcaa gtacttctga
     2041 atctacttct aatgtaagcg tagaaggaat gggatttgct attccaagta acgatgtagt
     2101 gaatatcatc aatcaactgg aaaaagacgg aaaagtgaca cgtccagctc taggtatcac
     2161 aatggtcgac ttatctgccg tttctactca gcaacaagaa caaatcttga agattccttc
     2221 atctgtaact aacggtgtca tcgtgacaaa tgtccaaaca gctacaccgg ctgaaaaagc
     2281 tggattgaaa caatacgacg tcattactaa gatcgatgac aaagaagttt cttctggtgt
     2341 agaacttcaa tctgctttgt ataagaaaaa agtgggagat tctgtcaaaa tcacttacta
     2401 tgaaggctcg aaagaaaaaa caactactgt tgaattgacg atcgatcaat cggcattaaa
     2461 acaaaatcaa aatagtgaaa attaataaaa aagaggttgt gacaaagact tgtcacaacc
     2521 tctttatttt ggaaatgctt ctccgactac ggcatcctca ggcaatacta agattccttt
     2581 ttttgctgga gcatttggca aatgcaattc acgagcggaa cagatcattc catagctatc
     2641 tacccctcgc aaagttcctg gccagatcat cattccatct ggcatcatag ctcctggttt
     2701 tgcaacgaca actttttgtc cagcttcgat attgggtgca ccgcaaacga tttgcaatac
     2761 tgtaccattg tctacttctg tctcagtaat cgataaatgg tcagaatcag gatgtgggac
     2821 gcatgttttt acgtacccga caacgatctt cggttcatga tcagcttcaa gctgttcttc
     2881 gaatcctgct tctttgatca atcgattcaa tttcgctaat tcctcatctg ttaagcttac
     2941 ttggccgttc ccttcgattg ttagatgatc ggagatatgg aaaaagttcc aagcaacaac
     3001 ttgaccttgt tcatctttga tccgagcaac attccatttg cgttcaacag tgttttctgt
     3061 tcctttatcg tcggctataa taaccatcaa ggtgtctccg acgtgtgctt tgttataagc
     3121 aaaaatcatg ttctttcctc cattatcctt tgctgagatt tatcttaaca aaaaattgca
     3181 ttcactgcat gaaatttttt tcatgcttta tttccgaaga agcctcttct ggaacaccat
     3241 ttattcgtaa caagggactg tgacaagact tttgtcacag tccctttact gttttagtta
     3301 attgattaaa aaggtcgtac aagttccact tgcttaatta cttggctgtt cttcttcgtc
     3361 agaaacgatc aagacatcac aaggtgaatg acgtatcacg tagctggcta cacttcctgt
     3421 cataaagcgt tcgactgcat ttagacctga ctgcccaacc atgattagat cgatctcata
     3481 tttttttggc aatttgactg ccatggattc acgaacagaa ccatatgcaa taattccttc
     3541 tatatttgtg taatcaacag atttcccata ttctttacat tcttcgatca tttccttggc
     3601 tgtttcagtt tgctgatcga ctagattttg atcaataggt gcatagccca taaagttata
     3661 aaattgctga tcgattacgt ttgctacata tactttccca ttattacggc gagccacttc
     3721 taccgctttt ttgaaagcag caagtgcttg ttcactaccg tcgataccta ctaaaatatt
     3781 cttatattct tgattcatct tgacccctcc taacagatgt cagccttttg ttacactttc
     3841 taggaacgat tcgatttcct gttttgtttt acgatcttta ttcactagac gacctaattc
     3901 ttttccgtct tggatcacga caaaacttgg gataccaaaa atgttccatt cagcggctac
     3961 atctatgtac ttgtcacggt ctacttcaat aaattgccaa tcagaaaagt cattttcaat
     4021 ttcaggcatt tgcggtttga taaaacggca gtctccgcac cagccggcag taaagaaaaa
     4081 tacactcttt ccagtttcta cgtagcctgc taattcttct aaactatttg ggataatcat
     4141 tctttgtcct ctactttctg tttatgtatt tttcaattaa attatacacc tttctttcga
     4201 actaacaaac attgatcctc tttttataaa aaaaatctgg caaaaagcaa tcttttgcca
     4261 gattttatag tttcactatc gcattatttt ccgtatacga tcgtatttac tgttgattta
     4321 tccaaacctt tcagtgtttg aatcaacatt tctcgtgcag cttcaaaatc gcgaatactg
     4381 aacatcgttt gatgcgtatg gatataacgt cccactacgc caatcactgt acttgggatt
     4441 ccttcgtttt gtgtatgagc agcacctgca tctgtaccgc cttttgaaac aaagtattga
     4501 taagggatat tgtttgtttc agcgatatcc agcaagtact cgcgcaaacg aggaagcatg
     4561 atcaaacctg gatcttggat acgcatcaat gtaccttctc ctaaatgtcc gaatgtgcca
     4621 tttttcgtca ctgtatcatc tgctgcagag cagtctactg caaagaacag atctggcttg
     4681 aatttattga cagaagcttt agaaccacgc aagcctactt cttcctggac attcgcgcct
     4741 gcaatcaatg tatgacctaa tgtttcattt tgcaacgctt ctaaagcttc taataccatt
     4801 gtacagccat agcggttgtc ccatgattta ctgataatat tctttccgtt ggctgttttg
     4861 atcgtttcag cgtaaggaac gattgtgtct cctggaagga caccgtaact cattgcctct
     4921 tctcttgatt caaacccagc atcgaacaaa atatcggtta cttctactga tttttgtccg
     4981 cttgttcctc gcaataaatg tgggggaacg gaagaagaaa tacatggata attcttacca
     5041 gtagaggttt taagtgtaaa acgctgagca gaaacaacgt aaggattcca gccgcctaga
     5101 ggtacgactt taaaaagccc gttttcttgg atttgtgtca gcatgaatcc gacttcatcc
     5161 atatgtgctg cgaccattac acggggtgca tccgcttctt catgatggcg cagaccaaaa
     5221 atgccgccca aaccatctaa ctgtaactca tctaccagcg gttccatatg actttccata
     5281 taagcgcgaa catcttgttc aaaaccgctt gttccttgta attctgttaa ctctttgatt
     5341 cgttgaaatg ttttttcttc catatgctat tcgtctaaat ttcagactaa actcctctca
     5401 gacttatatt gatctgcgga taaaatatga ttttttctgc agaccgatcg tatttattat
     5461 agcttatttt ttctggtagc ggttatttat ttcaaagaaa atatgagagt gtggtaaaat
     5521 aagcacaagg agtaacaagt gagaagaagg aggtatgtcc acatgtatga agaaagcgaa
     5581 ctaagctatt ttaaaggcgg gttagcagtt ggaatcggtc taggattatt gagcggggta
     5641 gcttcaacga tttggtatca tcgcaaaaaa gtattgtcag cagaccaagt acttgaaaat
     5701 atcaagtcat cttttctaaa agaaggtgca atcgaaggtt cttggatctc ttttgaaaag
     5761 gaacctatta ggaaattcgc cgttcggtcg agtgcttatc ggggaggtct ttctcgaata
     5821 gaagatggcg aaactgttta ttatgaattt ttagcagatg ctcatacagg aaccgtatta
     5881 gaaatttcaa gaaaaaaagg tgcaacagat taatacgtca aaaaagtgag agaacgaata
     5941 cgcacccact attcaaaaga gaggtatcat gtccttcttt tgtagtgggt ggttttttag
     6001 atagagcttt ccaaacatat attccagtat tttcttttta aataaatgat atttagcggg
     6061 aaaaactagc atttcctttg attatgaggt atttttaatt tagcttttgt tttctatagt
     6121 taaggagagt gaaaaatgga acaacaagaa aaaattgctg ttctgattcc ttgttacaat
     6181 gaggaagcaa caatttcaac agtcattgca gactttaaac gagaattacc agaagcagat
     6241 atttatgtct atgataataa ttcgaccgat cgaacatacg aattggccgt tcaaggtgga
     6301 gcaatcgtaa aaaaagaacc tcgtcaagga aaaggaaatg tgatccgcca aatgttcttt
     6361 gatatcgatg ccgactatta tttaatggta gatggcgatg atacttatcc tgcagaagct
     6421 gtacatggtt tgctcgagaa attacgttct ggtgaagcag atatggtcat tggtgatcgc
     6481 ctatcaaatg gaacctactt tgatgagaat aagagacctt tccacgattt tggaaataac
     6541 cttgttagaa atacaattac tagaatgtat aaaacaaaaa ttcttgatgt catgaccggc
     6601 tatcgaggat tcaatcgtat ctttgtcaaa agttttccta ttatgagttc tggttttcaa
     6661 atcgaaacgg aattgacgat ccatgcttta gataaaaaat tcaagttcgt tgaaatccca
     6721 atcgattatc gtgatcgtcc tgaaggcagc gaatcaaaat tgaatacatt ctctgacgga
     6781 ttcaaagtga tcatgatgat tattaaaatg tttaaagatt ataagccttt gcttttcttt
     6841 gggatttgga ccatcttctt ctttttattt ggattggtca cggggattcc tgtgattcgc
     6901 gaatatatgc tgacaagctt tattacacgc atcccttctg cgattttaag tactggattg
     6961 atgactttag cacttctttc attagtaaca gggttgattt tggatacagt cgtcacgaac
     7021 gctaagaaag aatatgaatt gaatctatat catgtttatc atgaatacca cagtagaaag
     7081 taaaaaaagg atcaagactg caaaaataat ttgcaattct tgatcctctt tttaattaat
     7141 tatttcgcca aacgatagat ggcatctgca tagattgctg ttgctctgaa caagtcatca
     7201 agactcataa attcgtttgc ttgatgcatt gtatcagtat atcctgggaa catcgctcca
     7261 taagcaacgc cacgtttcaa caaacgtccg taagttccgc caccgatgat ttgttcgtag
     7321 cctttttctc cagtgtggtc ttcatagact tgcaataatg tagccaccaa tggatcatcc
     7381 attggtacgt aatgtggttc cattacgcga gcacctcgag taactgttgc gccttcttgc
     7441 cccactgttt gttcgatgtc actttgcaat ccgtctactg ttacaccttt tggaaaacgg
     7501 aaattcatat tgatgaagtt cgcttcctct gtaccatttt cttcaaaagc aaatagtcca
     7561 gcattcattg tcaatttgcc cattttttca tcttcataag cgactcctag tttttctcca
     7621 taaaaatctt catggacata agcagctgct acatggataa attgtttagc agatcctagg
     7681 aattcatagt tatctaagaa caatgccaag aaagaacctg cattgatacc agattgtggg
     7741 cttgcaccat gtgcgccttt accaactaat tcgattttta catacgtatt gtccgcttca
     7801 atcgttccgc ttactggatt tgcttcgatg aattggtaaa atgcttcttc catcgcgata
     7861 gctgcatctg cacttggtac ttgaagtgct gcagttgctg ttcctggcac catattttcg
     7921 cgaagtccgg aaacaaaaga tttcaagaca taatctcctg cattgtcgcc tttgaagcgt
     7981 aaagctaaag aaacatttcc tttttcacca ttgatgattg ggaattcagc atctggtgag
     8041 aaaccaaaat ctggttcttc ttcatgttcg aaataataag ccatatcgcc ccagccgctt
     8101 tcttcatcgc tgccgactac gaagcgaact ttttttgata gtttcaagtc aagttcttta
     8161 atgatcttca tcgcatagta agctgccata cttggacctt tgtcatcgct tgatccgcgt
     8221 gcaaagatct ttccatcctt gattactggt tcataaggat ctgtgtccca gccatctcct
     8281 gcaggaacga catccatgtg tccaaatatc cctagcgttt cgtctccttc gccaagatca
     8341 atatgccctg catagttatc gacatttttt actttaaatc catcacgttc gccataagct
     8401 aacatatgtt tcaatgcgtc gcgaggtcca ggtccaaatg gtgcatctgg tgttactttt
     8461 gagtcatcgc gctcgctgtt gacacgtaaa agatttttca agtcttcaag caagtcttct
     8521 ttacgtgctt ctacttcttt ttgccaatca attgtcataa agttaatcct ccacttattt
     8581 aagtcttcgt gttcatgata ccacaaatta cagaaaaaaa tagttcaaaa aatctgacaa
     8641 ttccttttct tatctgtttt tctctaaaaa aaatatacgc tataatgagt aaaaggaggt
     8701 ttttagatgc tgataaacga acaactagtg aaaagagtca tcacacctcg tccaaaaatt
     8761 tcacataaag gaacatttgg acgtatcgtt ttgattggcg gcaatgaaca atatggcgga
     8821 gcaatcatta tgagtgcttt agctgccgta cacagcggag cgggtctcac gactgtagta
     8881 actgatgaaa aaaatcggag tgctcttcac agtcatctgc cagaagcaat gattgtagtt
     8941 tggacggata ctgaccagtt tctttccgta ataaagcaag cagatgtact cttgattggt
     9001 cccggattgg gtacttcttc taagagtttg gaacgtttca aacaagtact agaaaaccaa
     9061 tcaaaagaac aatggctggt gattgatggc tctgctttaa gtttacttgc tgctaatcat
     9121 cttttgctac cctatcccga acaaactgta cttacacctc atcaaatgga gtggcaacgg
     9181 ttgagttctc taccgataca agaacaacgg gataaagaaa acaaaaagaa gcaacaggaa
     9241 atcggtgctg tgctcgtttt gaaaagtcat gagacaaaaa tttatggtca ccagcacttc
     9301 tacaccaatc ctttaggttc tgctgctatg gcaacgggcg gcatgggtga cactttagct
     9361 gggatgatca caggattttt agcccagttt cctcaaaatg accaaacgat tgcagcagct
     9421 atttatcttc atagtttcat tggagaccag ctggcaaaag aacaatatat tgttttgccg
     9481 acacaaatca gcgaagcact tccgaaatgg atgaattatt tttgtaaaaa atcaatggaa
     9541 aacgaggcat gagtcatgga gaaatttaca ggttatgtgc gagaaccttt ttattatgaa
     9601 acagatcaaa tggggattat tcatcacagc aattatatcc gctggtttga agaggctcgg
     9661 gtagccttat tggtacatct agggtttccc tataaaaaaa tcgaagaatc tgggatcatc
     9721 atccccgttc tggaagtaaa ttgcagctat aaagaaatga tccattacgg agaaaaagtg
     9781 agcatccatc caactatcca aaagtatacg gggacacggt tggattttag ctacgaaatt
     9841 gtgggaactg aagacaaaaa attaaaaact actggcagta gtaaacactg ttttttactt
     9901 tctgattcgc aacgattagt gaagctgagc aaagtcaatc cggagttaga tcagttattc
     9961 caagcgtacg cttctttgga tgatgtaaat tcttaataaa gaccgtctaa gcatttttct
    10021 tgtattgtgt cgagtagttc gtataatgaa aagaaaaaag caggtgatcc ccatgtatgt
    10081 cgttaaagta atgcacggtt acatcgataa aactggctgt cgcactcggg aaaaaaactt
    10141 agacaatctg ctcattttca aagacaaaaa agaatccgaa gcttttgcta aaagaatcgg
    10201 cggtcgtgtc aaaccgatcc aagaggtacg accagattga ttcaatattt ttcttttcga
    10261 ggctctaata ggcatgccta tcagggcttt ttttgcagac tgtgttttta tgcccacacg
    10321 atttgtaagc gatttatgtt ataatagatt atatctgcat agaaaggaag gttaaatggt
    10381 tatcaataat ttgcaacagc aagcttatga atctatacgt aaacaaatca tttattccga
    10441 gttagcacct ggaagtaaaa tctccgataa agtgttagaa gaacaactag ctatcgggcg
    10501 tacaccgatt cgagaagcac tgatccaact gcgcaatcaa gagcttgtaa aaaccattcc
    10561 acagtccggc acttatgttt caaaaatcga tatccgatct gcttcattag cccgttatac
    10621 gcgggaaaag ttggaaaaac cgatcctgca agaatgcagt gcgaaaatga ccaagcaaga
    10681 tcaagctaaa ctagaaaaaa tactagaaca aaccgatcag gcagtaatcg ctcaaaataa
    10741 aaaagctttt tttgatttgg atactgattt tcaccgaact tgttatgaaa ttgctggaaa
    10801 acgagaaatt tgggattggc tagaaagtta tagtacgcac ttgaaccgtt tcagatggtt
    10861 acgtttgacg atttcagaat tagactgggg tcgagtatta gatgaacatc agacaatgct
    10921 tcaatcaatg attgatcata actttgatga agtcggattt ctttgcacga tgcatttaca
    10981 tatgattatc gaggagcaag aatacgtgat tcataattac cccgattatt ttgaagatat
    11041 tcaggacaga cccatcaaat aagaggtttt ggaatcagcg ttttgacctg agtattagag
    11101 aaagaaacgg caaaatagtt cctcatattt tgccgttttt tttgagctaa taccgagggt
    11161 cagctgattg cacaccgttt attgagaggt tgtgaaaaag ctgccctgca tcaagtaata
    11221 agaacagcca agtaaaaata gcttcttatg tttttaactt ggctggactt attaccgcag
    11281 atgcaagctt ttgaacaccg ttttccaaga ggtcgtgagc ctgccgttca tctccgagtc
    11341 acaaggaaca atttcaaaaa acagcttctc atgtttgttg aatattttga cttgtgaccg
    11401 aggagttgac aggagaacat cgtttattcg gaaaaaaagg actgtgacac gacttttgtc
    11461 acagtctctt tatttgtaat ctaaatcata ctccgtacat cttttttcta ttttttttat
    11521 ttcttctaag atcccttggc tacgaatttc attatatggc caaagtaaga ctgcctctaa
    11581 tacataaggc gaaaaatcgc tttgattcat ctcttttgaa aaactcatcg ctgctatctc
    11641 gctcaatgca cgaatgtttg attgatgtgt ataaaaaggc aaccgccaca gcacaatttt
    11701 ttcgctttgc gtataaattc ccttaacaga agcttcgatg tggtgaaata gttcatgtcc
    11761 aataatcaaa gcttgcgctg tttctttaga gatcgaagct agtcctgaac attttaattt
    11821 ttctaacggc tccgtcatca atcgaatccc ttcatcttct gcataagttg caaaagtgat
    11881 tcgttcgttt atttgctctg attcacagga aacgattgga acagccattt gttcacaaat
    11941 ttctcctaca tcggcaaaag ggtacttttt tttgatgcta catgccgttt cgataccaca
    12001 atcgattgat tgttgtataa gaattttctg cttggacaaa ggaatctttt tccaaaataa
    12061 atctcgccga aactggtaga atccccaaat ttcttcgggg atctgttgta attcgtttag
    12121 taactcttct aacttatgtt tgtttgatat ttccggcaac gacgatcact tcttcctccg
    12181 gttccatagt agatacttcc agatccatca aaagctgtag ttgttccaga tccgtcgctg
    12241 taaaatcaga gactcttgct cctaagcaga gataaaattc tggtcgggca atcaattcag
    12301 tttgataaac tgccatatct tcccaaagtt gttcgacagc tgtcatataa ttggctgcag
    12361 ttgttttcat acatgaagca tgtccacgtt ggaccttgat aaatcctttt tgatcgatga
    12421 tccgaagact tccagcattt tttgaacgat tctgttcgcc gcatacataa aagaaaggtg
    12481 tattttccaa taagcaaact tcatttttat ttaatcgcat atcctctgca gcaagctcta
    12541 gtgcttcttc tgttgtgatc tccttggtta gatcggttgc tttcacttcc gtagaaccag
    12601 tagcgatagc cgttactttt gaagtctgtg gatcgatttc tacgtggact tcgatcgatt
    12661 ctggtgtagc gcctgattca atcgctttat tcatcgcttc atttttcaac gatcgtatat
    12721 cttctttact tggcgaaggg atgatacgtt ctacaacatc tcggaccatt gcaagtgcta
    12781 caccaataga agaaatcact tcggcgtttt caggaatcga atatttcaca cccattttat
    12841 tactaaagta agtaatcaat gaggcagcgc cgccccctac acctacaaga gaaatctgat
    12901 ctttctccaa gtgatacttg tctgccaatt ctaaaatcac cggttcaatc ttagcatagg
    12961 atttttccat gatctgttct gcgatgtctt caaccgtcgt atgacaataa tcagctaatg
    13021 cttggatggc ttttcgtgca gatgggacat ttccataaga aaaatgttct tcttgcacta
    13081 atccaaggac attggctgca catgtatttg taatagtcac ttcttctccg tcttccatca
    13141 ctaccttgac gtaatcagca gggtctcctt ctttaggaga gaaaaattca accttaggac
    13201 ctttgatttt ttctgtctcc gtaaatactg cataatctag accggcaata tgtgcagaac
    13261 gtggaccgac atcgatgatt cctgactgat tagcccgtac catcgaaccg cctgccacac
    13321 ctagaacccg aacatctaat gaagagatat atgttgggtg gccacctaca attgaataat
    13381 cgattgctgg acgtccattt ttaatgaccc cgatattggt tgttgttccg cctacttcaa
    13441 aataaacacc attagacgct ctcaagtaca tcaaagagcc catgactgat gctgctggac
    13501 cggaaagcat ggttaaaaca ggtcgttttt tcatttcatt gatttccatt actccgccgt
    13561 ctccgcgcat aatcatcaaa gaaacattga cccctgcttc tcgtacggaa tcttctgtag
    13621 aggttgccgt atctagcatt tttggtaaaa tagatgcatt gattgctgct gtccgagttc
    13681 ttcttgtcaa tccatataat tttgtgatgt cagaagccat cgttgtaggg atcgatttta
    13741 ccgatgctgc ttcataaaca actcgttctg gttccccatt atctactcca aatgccatag
    13801 aagaaaccaa gacctccgct ctttctcttt ctaaagaaga aatcgtttct gaaacacggt
    13861 ctacatttaa gtgtttgaca ggtaagaatg cattgacaat ctctattttt ttcttattcc
    13921 ctaaatcgat atcgttcaat ctagtttgtc gttttgctaa aaaaccttct aatccacctt
    13981 ttgccatccc gattacgccg acttttgcta cgtctccttc aatcagtgca ttagtcgctt
    14041 gtgtggtaga atgtgcaaca aacacaacat cttctggact gatattattt tccagcaaac
    14101 aattttgaaa agactgaacg acacctgcgg ctactccacg gacatcatcg tgggttgttt
    14161 tcactgaaga ttttccgatg atttcgtgcg tcgcattatc gattgcgact gctttcgtat
    14221 gtgtaccgcc aacatcaatt cccattcgca caaaacgttt cattttcttt tcctccattc
    14281 atctaatcgt ttttatcgac gttttgttta ctaggcgttc ccgtacaata agaacgtgat
    14341 caggcataag atcgctccga tgatccaacc agttgggatg gatttcttca tatattctcg
    14401 agaagttact tttgtataac ctaatcccca agcaacccaa gattgtgtaa tatctaaatg
    14461 ctgaggagca atcgttgtga ttgcaaataa cgggaacaaa aaggctggcg acatcgttgg
    14521 attaacagct aatacgacag ctaaaatagc tgagcctgaa ccgactagat tcattggacc
    14581 gcggaaaaat cctagtggtg tcaatatagc aaatacgatt gccaagacta atggtgattg
    14641 cggtatccaa tctccaaaaa tagctgaaaa ataaggagaa gcatacgcag ctgaattatt
    14701 gaacatcgct aaggtcatca agaaaccaac cattggcgca acatctactg cgccatctgt
    14761 aaataatttc gcaaactgac gacagatttc tttataacct ccgcgcaatt ttccagtaaa
    14821 cagcaatgcc cagatacctg ctagaataaa acctaagatg attggaagat ctaaaagaac
    14881 gacccctaaa actggtaata atacggctaa ccatgagatc attggtgcgt cgccaccttc
    14941 agctggtacg tttgctgcca ttgcatagcg tttctttttg ttcattgata tattagcgac
    15001 cgttagtacc acaactaagc ttacaatcat cccaatcata ccaatttgga aatagtcatt
    15061 ataagtatag ctttcagccg ctggattgaa accagcataa atcgtttgat attgtttgaa
    15121 attgacgata ttagcaaaga ttccagccat aatcgagccc ataaatgaga acagtgcaac
    15181 cggtactgaa atacctaatg acatcaagat tggcaacaca ataactgcaa tagaaatcac
    15241 tggtccgatt cctgtcatcg acatgaatat aacagcggtt acaatacata gtaagctcat
    15301 cgtgattctt ggcttgtccc ctccaagttc aactaccttt cgaatcaggg tagctgcgat
    15361 tccgctatct actagaacac gcccgaaaaa ggcaccaaag aagacattta ccaagatcga
    15421 tttggcgtac tcagctggcc ccgtcgtata gatatgcgtc aacacatcaa ttactccttg
    15481 cccttccatc gccgggtttg gtgaaaacgt gtttccgact agcgctaatc ccatccatac
    15541 agtagccatg atggcaaagc cgaccatcaa gttgtagcct tttacacagt accagaccat
    15601 gaaaaagaat gagataacta acaaaatacc aataataata tcactcatgt tattcctccg
    15661 tccattttgt ttttctgata agcaaactaa tatatcagtc aaactcattc taacttatgt
    15721 agttagcgct ttcattaaag ggtgaccata aatgttcagt tttcaattaa aaaaccttaa
    15781 ttatgctatt ttagacttat acgttattgt cctgtttcat ttgatggagg attgtaaata
    15841 aaaaaagagg aaagaacata acttttcgtt atcttctttc ctctttataa ttgtttagct
    15901 tatttagcca aagcttcttt tgcttggtca gccaatgcag taaatgctgc tgcatcgtta
    15961 acagccaagt cagctaacat tttacggttg atgtcgattt cagcaacttt caaaccgtgc
    16021 attaattttg agtagctcaa gccattcata cgagctgctg cgttgatacg agcaatccac
    16081 aatttacgga agtcgcgttt cttttgacga cgatcgcgat atgcgtagtt gtatgaattc
    16141 attacttgtt cttttgctgt tttaaataaa gtgtgttttg aaccgtagta accttttgct
    16201 aattttagca ctcttttacg acgtttacga gttactgttc cacctttaac acgtgccatg
    16261 tataattcct cctaataaaa ataaatcttc agattctgtt atttgtactt cttatttcat
    16321 ttttgctagt tgttggcgaa tacgtttgaa gtcgccacta gaaaccatgc ttgctttacg
    16381 caattgacgg cgttgttttt ttgttttgcc gtggaaacgg tgacttgtaa acgcgcggaa
    16441 gcgtttcaat ccgcctttac cagtacgttt gaaacgtttt gctgatccgc ggtgtgtttt
    16501 ttgttttggc atgactatat ttcctcctca aaatctttcg ttatccgact atcggctgtc
    16561 caacagccag ttagtcattc aaattactta ctgtcgtttt tcggtgccag cgtcaagaac
    16621 atgctgcgtc cgtccatttt cgctttttgt tccactgttg caatatccgc agtttcttca
    16681 gctaagcgat tcaagacttt ttgaccaatt tctttatggg taatggcacg gcctttgaaa
    16741 cggatagaag ctttcacttt atcgcccttt tccaagaact tacgtgcatt acgaagtttt
    16801 gtattgaagt cattcaagtc gatcgtagga cttaagcgaa cttcttttac attgatcact
    16861 ttttgttttt tacgcgcttc gcggtctttc ttttgttgtt cgaaacggta tttaccgtag
    16921 tccatgattc gcgcaactgg tggtttcgca ccaggggcaa ctaacacaag atccaagttt
    16981 gcttgttctg caatattcaa tgcttctgct tttgtcttaa ctcctaattg ctcaccatct
    17041 gaaccgatca atcgtaactc tcgtgcacga atgccgtcgt taaccatcat atcctttgct
    17101 atggtcattc acctccaaat attatgagag aaagaaaagc ttaccgaata aacggcaata
    17161 aaaaaacgag ttctcttaca gaacccgcgc gtacttcacc taatagaacc agtgaaaact
    17221 atctagccca gtgacattat caactaaggc gagaagcggg tgcttctgct tttatttctc
    17281 aaccatcata gtatagcaaa aactaatctt tttgtcaatg tttttcatac agtttgtctt
    17341 ccttttctga aaatgattag attagcagac tttctagcag tgattc
//
LOCUS       DACCCS010000055        17134 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.056, whole genome shotgun sequence.
ACCESSION   DACCCS010000055 DACCCS010000000
VERSION     DACCCS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.056"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(21..1040)
                     /locus_tag="IVK82_002062"
     CDS             complement(21..1040)
                     /locus_tag="IVK82_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6393108.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1062..2069)
                     /locus_tag="IVK82_002063"
     CDS             complement(1062..2069)
                     /locus_tag="IVK82_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP6393109.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2062..2727)
                     /gene="pgmB"
                     /locus_tag="IVK82_002064"
     CDS             complement(2062..2727)
                     /gene="pgmB"
                     /locus_tag="IVK82_002064"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP6393110.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2720..5014)
                     /locus_tag="IVK82_002065"
     CDS             complement(2720..5014)
                     /locus_tag="IVK82_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP6393111.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5274..7439
                     /locus_tag="IVK82_002066"
     CDS             5274..7439
                     /locus_tag="IVK82_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6393112.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7743..8576
                     /locus_tag="IVK82_002067"
     CDS             7743..8576
                     /locus_tag="IVK82_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP6393113.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8678..10378)
                     /locus_tag="IVK82_002068"
     CDS             complement(8678..10378)
                     /locus_tag="IVK82_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393114.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10885..12045
                     /locus_tag="IVK82_002069"
     CDS             10885..12045
                     /locus_tag="IVK82_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6393115.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12061..12504
                     /locus_tag="IVK82_002070"
     CDS             12061..12504
                     /locus_tag="IVK82_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6393116.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(12987..13517)
                     /locus_tag="IVK82_002071"
     CDS             complement(12987..13517)
                     /locus_tag="IVK82_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP6393117.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13622..14173
                     /locus_tag="IVK82_002072"
     CDS             13622..14173
                     /locus_tag="IVK82_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6393118.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14326..14799)
                     /locus_tag="IVK82_002073"
     CDS             complement(14326..14799)
                     /locus_tag="IVK82_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6393119.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            14937..16409
                     /locus_tag="IVK82_002074"
     CDS             14937..16409
                     /locus_tag="IVK82_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6393120.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16406..17116
                     /locus_tag="IVK82_002075"
     CDS             16406..17116
                     /locus_tag="IVK82_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP6393121.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5323 a         3083 c         3522 g         5206 t
ORIGIN      
        1 gtatttagtg agtcagctat ttagtatggg ttttctaaag gaacgactgt ctctcttttg
       61 atcaagcgat gagggatgac caaacgtacg ccactgctca attcttcatg gatcaattcg
      121 cgcagctttt gcatagcgac acgtcctaat tcggagatat cgatatcgat acttgtaagg
      181 taagggtgtg ttaatgttga gaagatcgag ttgttgaagc taatgacgga gagtgtctct
      241 ggaatgacat agccatacat ttgcgccaac tgcatcgtgc gaagggcgaa gatatcgtca
      301 atgaccacca atgctgtggc ttcgttttct ttcaacgttt tctcaaagga cagataatcc
      361 tccggatgct ctagtactac actggataac gcaggaagac cagccatcat cattgctttt
      421 tgataaccga aatatcgttc gaaatacaaa ctttcatgag tcgtgtttgt cacaaacagg
      481 atattcttat gtccattctg gatcaaaaat tctgttgctt gtttacctag aagctggtta
      541 tcattatcta cataaacgat ttcgttctca tgatgataag gctgtccgat caaagtgaaa
      601 gggatgtggt tttggtataa ataatcaatg acagtatctt gactatctga atacactaga
      661 ataaaaccat ctacttgttt ttgtaaatgc atacgctgga cgttctctaa cagcaattca
      721 aaatttttag cagtagcgat tgctgtagtc atatcataat tccgcgcttc ttcgttgatt
      781 gcttccatga tctcaagata aaatgggtta ccgatccgct cttttgaatc aagagggggt
      841 aagattacgc cgatggcact ggaaatgcgt tttcctagat tgcgggcagt catgttagga
      901 acataaccta gttcatccat aatttttcgg actttcttct ttgttgtctc ggaaatacta
      961 ggatgatcgt tgatgacacg agagacggtc gaggtggcaa caccggcttt tttcgccaca
     1021 tcttttacgg taatagccat gataaaaaca cctttctctt gttattgtgc agagaaccga
     1081 taaatcgtct gactgatccg ttcttgacca ggatggaggt tgatcgatcc aaatttgggg
     1141 aaatgaacga tatcagggat ttcttgcggt tcgattgcta atccgtagtt tgaatgcatc
     1201 tgttttccat tgacagagaa atcagcttcg aatccagtcg tagaaaataa taccatactt
     1261 ttattagaag ttgctatagt catttggcga ccagaatgct tctcgaataa ttgtaaggaa
     1321 gttttggaaa gcctaggatg atgcaaaaga aatacatcat ctagtccatc tggataacat
     1381 gcaaggatct cttgcagtgt tggggatttt ctaaagtcga ttggcagttt ttcttttttt
     1441 gtccaatttc cagtaggcag tttttcttga tctaattcaa gcacaccatc taaaaagacc
     1501 gttaactgat gttcatagat gtctcttgtt ccatttcctg aaaggttgaa gtaggcatgg
     1561 ttggtcggat tgcagatggt ttcaacaagg ctactagcag aggttgtcat ctctaattcg
     1621 ttttttgtta aacggtaagt gatcgtagca gtgattggtc ctggataggt agaaaattca
     1681 tcttttaagt aaaagaccac acctattgat gatggagttt tgaaaacttc tacttcccaa
     1741 aattggaaag accagccttt cgtgccaccg tggatatggt gtgatccgca gtttttctcc
     1801 aatctatgct caccccaaga agcatcccgg atccttccgg ctacaggtcc gacagttgct
     1861 ccaaaaaagc ttttgtctgt tagaacatct tcgaaacgat cgtaagacaa taaaacattt
     1921 tctgattttc cttctttgtc tggtgcgaag atttggtgca ggcgggcacc gtaattgaga
     1981 agaacgactt ctaagtagtc gttcttcaat gtgattttct ttatttcagt ctctggaact
     2041 ggtacgacgg agatctctgg attatccatg gtcatgccac ttttctttca agtaatcgat
     2101 cgtaagaaaa gtcgtatcag ggacaagcgg gatctcctct ccaaggtctt cagcacgtcc
     2161 cacgccaatt ggttgagcgc cacttgagag gatggcttgg ataccggctt gcgcgtcttc
     2221 gatcccgata cattcttctg cttttagtcc gacagctttt gctgcaagga ggaagatatc
     2281 tggcgcaggt tttccgtttg ttgcttctgc cgggtcggca atagcatcaa agtaaggtgt
     2341 aagacccatt ttttcaagta ggaatggccc gtttttactt gcagaagcaa gagcaatctt
     2401 gattttttct tcttttaaag aatccagtaa aggcacgacc ccggggaaga catcttttgg
     2461 ttcgatcgtc tggatcatct ctaaataata atcgtttttt cttttcgcta attctgcaaa
     2521 ttcttccttt gaaaaagagt gttcttttcc gccataagct aaaagcaaag agagtgagtc
     2581 ttctcggctg acgcctttta gctgttcgtt gaatgtgtgg tcgatcgtaa tgccgatttc
     2641 ctgaccaagc tttcgccaag cacgataatg gaattcagca gtatcggtaa tgacaccgtc
     2701 taaatcaaac aatacccctt taaacatttg cttcctcctt tcttagcgtt atttctagac
     2761 gatccgctaa agaataagac tgttcataga cgtaaatttc tagaggagaa ccttctaata
     2821 actcgaaagt aacttgttca tctgtgactg agatgaacaa taaacgtccg cgataattga
     2881 tatggaaaga ataagctttc caagtttttg gcagaaatgg tgcgaatctc aactgatcat
     2941 gggcagtttt catttgagca aatccttgga caatcgacaa ccagctacca gtcatggaag
     3001 tgatgtgcaa gccgtcttct gtgtcgttat tgtaattatc caaatccaag cgagctgttc
     3061 gctggtacat ttctacagct ttgctttcca ttcctaattc tgctgctaaa atcgaatgga
     3121 tacttggcga aagggaagac tcatggacag tcattggttc atagaaatca aagttttttt
     3181 gcttttcttc ttttgtaaaa gtatcattga agaaataaat cccctgtagt acatctgcct
     3241 gcttgatgaa gcaagatcta aggattttgt cccaagacca atgctgattc agaggaagat
     3301 cgcttgtggg aatatcggat acaggcatga gatctttatc taagaatgtg tcatgctgga
     3361 caaagattcc taattcttcg tcaactggga agtacatgtt ctctgcgata tctttccatt
     3421 tggctagttc ttcttcgctg atagtgatag cagcttcttt ttgataagtt aaataatttt
     3481 ctcgcgtgta acgcaagacc caagctgcga tcgtatttgt ataccaattg ttattgatat
     3541 tgttctcata ttcgtttggg ccggtcacac cgtggatcat gtatttattt gctcgttttg
     3601 aaaaatggac acggtctgcc cagaagcgtg cgatttctgc taaaacttcc agtccttcat
     3661 ttttcaaata ttccgtatcg ccggtgtaat tcgtataatt gtagattgca taggcaatcg
     3721 cgccatttcg gtgaatctcc tcgaatgtga tttcccactc gttatggcat tcgacaccag
     3781 tgaaggtgac cataggataa agcgcgcctt ttaatccttg ttgctgagcg ttgtggattg
     3841 cttgcggcag ttgattatgc cgatatttca gcagattctt agttacttca ggtttggcta
     3901 aagcaagata aagagggaca gcatacgctt ctgtatccca gtaagtggct ccgccgtatt
     3961 tttctcctgt gaatcctttg gggccgatat tcaatcgttc atcttcgccg tagtaagtag
     4021 agaatagttg gaacaaattg aatcgaatac cttgttgtgc ttcttcgtct ccttcgatga
     4081 ccacatcggc aagcttccat cgttctgccc aagccgctgt ttgctcttct tttgcttgga
     4141 tataattgtt tgtctggttc gttaattctg agacagcttt ttctgcttgc tgcgcttctg
     4201 gaatatctct actagtaaga acgataattt ctttttccac tgaccatgtc tcaccttgag
     4261 ataattctgc agacaagcaa ccatgtaatg ctaaagtatc ttcgtgatag tcagggatga
     4321 ctgattttcc attaacgaaa tggcgcattg ctgcagtaac agtaaattgt tcaatcccaa
     4381 agttgtttgg aatagtttta gtcgtcaaat agccgatttc ttctatagtt ccacgatcaa
     4441 tctcattcca aaaatgttca tcatagttgc tgtcttcgtt ttgaacattt ccatccaatt
     4501 ttgatacgag tttgactgtt ccgctacctt caagcatttc tatagttaaa tagatataag
     4561 ctgattcttt tttgacaatg ctcagaaaac gttcgaagga aatgcgtact ttattagtag
     4621 aagtagtgat tgtgaatgta cgagataata caccattttt catatctaat tcccagtaaa
     4681 agtcttctgg ttccaaagaa gccaagtcga tcagctgatc gttgatcgaa atatctatcg
     4741 cgataaagtt caaggcgttg atcacttttc caaagtattc tggataacca tttttccacc
     4801 agccgacacg tgtcttatcg ggataccaga cacccgccaa ataagtacct tgatggtggt
     4861 cccctgagta agtttcttca aagttcccgc gcatccccat ataaccgttt ccaatacttg
     4921 ttaaagattc ttgcagtcga agattttttt tatctaattg tgtcgtgctg attttccatg
     4981 gatcaatagc aaatagtcgt ttgatctgtt tcatttttct cctcctaatg agctgtttta
     5041 tacgttcata ataaacgcaa ccgattgcac aagtcaatag acaaaagaaa atatttcata
     5101 gaaaaaagct taaaaaaata aatttttagg agaaaattat agaagggaac acacgtatta
     5161 taggttttat agaatggtat agacagaaaa ataaaaaata aagcatttac aaaaaagaaa
     5221 acgcacgcta taatacgttt aacgaaaccg attgcgtaaa ggagctaaac ggaatgaaaa
     5281 agatatttag ttttgagttt tggcaaaaat ttggaaaggc tttaatggtt gtggtagcag
     5341 ttatgccggc agctgggttg atgatcagta tagggaaatc aattcccttg atcaatcctg
     5401 attggacgcc gcttgtcaca acgggcggag taattgaaaa tattggttgg gcaattattg
     5461 gtaatcttca tattttattt gctttagcaa taggaggaag ttgggcaaaa gaaagagcag
     5521 gcggagcatt tgcagctggg atctcattta ttttaatcaa tcggatcact ggggctatct
     5581 ttggtgtcac aagtgacatg cttgcgaatg aagatgcatt tacccacact ctgttcggta
     5641 cgaagatcat ggttaaagga ttttttacaa gtgtattgga agcaccagct ttgaatatgg
     5701 gagtattcgt agggatcatc gctggatttg tcggggcaat ggcttataat aagtactata
     5761 attatagaaa attgccggat gctttgtcat ttttcaatgg aaagcgcttt gttccgtttg
     5821 ttgttatctt atggtcaaca atcgtggcat tgattcttgc tgtggtgtgg ccaaacgtac
     5881 aggctggaat caataatttt ggtttatgga ttgcagaatc acaagaaagc gcaccgattc
     5941 tagcaccgtt tttatttgga acattagaac gtttactatt gccatttgga ttgcatcata
     6001 tgctgacgat tcctatcaac tatacacagc ttggcggatc gtatgagatt ttatcaggcg
     6061 cacaggcggg gacacaagta tttgggcaag atccgttatg gttggcttgg gccactgatt
     6121 tagtgaatct taaaggtgca ggggatatgt cgcaatatga gtttgtgttg cataactgga
     6181 cgccggctcg tttcaaagta ggacaaatga ttggttcttc aggtatcttg atgggaatgg
     6241 cttttgcgat gtatcgtaat gtcgatcctg ataagaaagc aagatataaa tcaatgtatt
     6301 tttcagcagc gttggctgtc tttctaacag gtgtaacaga accattggag ttcatgttta
     6361 tgtttgctgc tgtgccatta tatgtcattt attcagtgat ccaaggggct gcatttgcga
     6421 tggcagatat cttgccattg cgggtccatt catttgggaa tatcgaattg ctgactcgga
     6481 caccgttagc tatcaaagcg ggtctaggtg gagatcttat caacttcgtg ttgtgtgtga
     6541 tcgcttttgg tgttgttacg tatttcttag caaatttctt gatcaaaaaa ttcaactttg
     6601 ctacaccggg acgtaacgga aattacgaca atgacagtga agaaaccgtt gtatctaaca
     6661 gcggaacagg aacagcagat cagcaagtgg tccagatcat tcatttgctg ggaggaaaag
     6721 aaaatatcaa agatgtcgat gcttgtatga ctcggttacg cgtaagtgta aatgatcgag
     6781 aaaaagtagg gactgaagaa gcctggaaac gagcaggggc gatgggattg atcgtcaaag
     6841 acaatggagt ccaagcagtt tatggaccaa aagcagatgt cttgaaatca gatatcgaag
     6901 atctgctgca atcaggagca gagattccag agccagaaat ggtttctaca ggaaatggac
     6961 aagcaggtag taagcaatac ttaggtatag aacaagaact agtttccgca gcttctggag
     7021 aagtgattcc tttatctgaa gtaaaagatc cggtattctc acaaaaaatg atgggtgacg
     7081 gctttgctgt tattccaaca tcaagagaag ttgttgcgcc gatcgctgga aaagtgacaa
     7141 gtattttccc tagcaaacat gcaattgggc tggaaacgaa agatggtatc gaagttttga
     7201 tccatatggg aatcgataca gtccaaatga atcagccagc ttttgagatt gctgtcaaag
     7261 aaggccaaga agtaggaaca ggaacgaaat tagctgagat ggatcttgat gtgatccaaa
     7321 acgaagaaaa agatccaacg atcatgatcg tatttacaaa cgataaagta gaagaagtag
     7381 tgatcaagca attaggtaca gtgacagctg gtaaagtgat cggctccatt aaaatgtaag
     7441 gagaggttgt gaaaaagctg tcctgcatca agtcataaga acagccaaac aaaaatagct
     7501 cctcctattt ttcgtttggt tgggcttatt accgagaagc agcagtttga atactgttta
     7561 gtggagaggt cgtgaaaaag ctgccctgca ccaagtgata aaaacagcca aacaaaaaat
     7621 agctcctggt ccattgagct tattactgag gagttagcag gagaacacta tttgctggag
     7681 aggttgtgac aaaaattttg tcacaaccct tttgtttcga aatgaagaca aggagaaaac
     7741 caatgaaatt catgacactc aacacgcata gctggcttga gccagaacca gagaaaaaat
     7801 tgcaagaatt agcagataag atcctgttag aagattatga agtgattgct ttacaagaaa
     7861 tcaaccaatt actggaatct gaagaagtag aacctgcgaa gctaatgaag ttctgtccgg
     7921 taaagaatca agtacctatc cgtaaagata attttgccta ccgtttagtt cagcttttaa
     7981 aggaacacgg aaaagagtat ttttggagtt gggaaatgag ccacatcggt tatgataaat
     8041 atgaagaagg aaatgcactg cttacgaaaa aaacattgga aagtcaggtc cttaccgtct
     8101 cccaatttca aaagaaagaa gattatcaga cacgcaagat cttgattggg aaaacaaaga
     8161 tagatgatca ggacattttt gtctcgagct gccacttttc ttggtggaca gataaaaaaa
     8221 gtggctttta ttttgagtgg aaaaacttgg aaaactactt tttggaaact cgcgtaccac
     8281 tttttttcct aggtgatttc aataatccag tagactctca aggttattat actgtaagag
     8341 aaagttgcct tcttcttcaa gattcgtatg ttgtcgcgaa tgaaaaagga aaagcagcta
     8401 ctgtggaaaa gaaaatagat ggttgggaac aaaatacaga aaaattgcgt atagatttta
     8461 ttttcgtccc tgaaggaatg caggttaaaa aatatcaacg gatttttgat gggatcgata
     8521 gtccaatcat cagtgatcac tacggggtgg aaatcgaatt agatgtgaac gaataaaaaa
     8581 tataacatat aacataaaaa aactgattaa aataaaactc tccaaacgat tataccgttt
     8641 ggagagtttt ttgatcggat gtatttatcc cttttgccta agcacgtaaa agcttgtatc
     8701 tatgataaat aaataaacat agcaaggtaa accagccaat caaagtaaga gaaagaatga
     8761 aatagaaata gattggcgtt tgataatgaa gcgtgagctc gttgattcct ttttgctgct
     8821 tcacgattgg ggtcccgata tctgttactt cataatcagt caatgttttt ccattttgct
     8881 gtagcacagt ccgatcgtag acgataacag ggatatgaac agttttgtcc ccagtgtttt
     8941 tccacttgat tgtcaaaaga ccgcctggat gtgtttttat aaaagttgtt tcgttttcta
     9001 gtatttcttc gttataccgg tcgtatttat tgtcttttgt ctccttatag attggcagat
     9061 agtcaggtgt ggttttttgg aaaacgaaca agctggtaga catatctttg ctgaagaatg
     9121 ttttccttgc ttcttccggc gtatcgaata gataagtatg tcgactgaca aatgtttcat
     9181 tttcccactt ttccagatgc tggtataagt tctggatcgt ctgtgctgaa aaaataagaa
     9241 tgccaaatat acttaaaaga cggaaccact tttgttcaga ccactggttc agtgaaaggg
     9301 caaggtaaag tagaagcaat agcgtaaatg gcacgaaaaa tcgaaatgga aactggatca
     9361 aactaataaa aggcacattt ttttcgatca atgtcgtcca tggaatcaag ctagtagaaa
     9421 gaaaaaagaa aacaagactt gtgtacaaca cgattttttg gaataaggtg ttcttttgtt
     9481 gaaagaaaat aaaaagcaaa aaacctgata atatcaatat aggaaaggcc ttagggtaat
     9541 ggagccaata actgccatcc agatctatcg ccttttgact catttctctg ttaacaaagg
     9601 gaggaagaat cttattccca ctgtaaacaa tagctaatcc tgcccaaata tttgctgtca
     9661 gaacaaaata aagcaagacc gacttaatca gttgaagcag tgcttttcct ttttcagtct
     9721 ggttaaaaaa gtaataaaga taaaaaggga tatacatcaa agctaacatg atcgaagaca
     9781 gtaaatggat ctgtgtcata agtcccactg agacagcgac tttgataaca gggaactttt
     9841 tatgaaggac gaaatcagca atagggatca tacataaagg ataaaaacaa gtcccccagc
     9901 tagtgaatcc ttggttgata gaccagtatt gaatagaata cgtactcatg aataaaacgg
     9961 ctactggtac acttacccaa tttcgaacat tgacatatct cagtaaaatc agcatagaaa
    10021 atcctgaaat aagataaaga atgaaatttg aaagaatctg ataatgaaac cagctacctg
    10081 caagcaacac gatcaatcct tgtaaataag cgaaaaatgg gccgtaaacg gcattcacga
    10141 tcctgccact ttgctggaat ccataaaggg aaggaaaata atggaaatcc ccatatttaa
    10201 tttgttgcgc ggcatcatag aaccgattgt aatggaatgg ggcatcagca cccaaaatta
    10261 gtttatgtgt gaataaatga ggcatgagaa atgccaaagc aatgaaaaaa attgctaaaa
    10321 atgggccata ttttttcaat tgtcgaactc ctttgcaaac tttagataaa ttcatcatat
    10381 atcctatgcg agaggtaggc aagaaattag aaaattcatg aaaatattta tgaattttac
    10441 aagaaaaaca taagtgaaag ttctaaggag agaggatgcg acataagttt tgttgcatct
    10501 ccttgttttg aaacagaatt tttcttaata ctcgaaacaa agtagctttt tcatgacctc
    10561 tcggtaaacg gtgttcaatc agctgcttct cggtaataag ctcactagcc tagaaaatat
    10621 gagaagctat tttctgacta gcgctcttat tactcaaagc ataacgcagt tttcacaacc
    10681 tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagcccaac ccaacaaaaa
    10741 atatgagtag ctattttttt gggctgtact tattacttga tgcaggacag ctttttcaca
    10801 acctcatcgg ctttcatttt ctattttttt cctgaaccca tataataaaa gaaagaacaa
    10861 aaaaacttgc aagggaggac acatgtgaag cagagtaagc aaaaacagtc tcgtcgactt
    10921 ttgtttcgtt tgtttttatt aatatgtatc gtcgtagggt tactgatcgc tttatatcca
    10981 ttttatgtgg attctttaaa tagttttatt gatcaaaaac gtatcgaaca agtacaaaaa
    11041 cggaccgcag ctgaaattga agtgcagcga aagaaaatgg aggagaaaaa ccaacgatta
    11101 actgaccaag gattcaatcc tggtactgat cctttcaatg aaaagaacag aaatgaaagt
    11161 acaacatcca gccaattaga ggaatggctg atcggaagta tcaatatacc taagatccaa
    11221 ttaaatattt cactttatga tcggctcaat ggtaggatcc ttgaaaatgg agcaggcgtc
    11281 ttgcaaggaa cctctttccc gcttggtgga aattcgacgc attcagtgat ctctgctcac
    11341 agcggtttac ctaatcgacg gttatttacc gaattagatc gtttagaaaa cggagacacg
    11401 tttattttga ctgttttggg ggagaaattg gcttatcaag ttaaaaatat acaagtggta
    11461 ctgccagatg acacgtctgt tctaacgatt tcagaaggag aagacttagt taccttgctt
    11521 acatgtacgc cgtatatgat caacacgcat cgtttgctag tgacaggaca tcggattcct
    11581 tataacgaat cggtaaaaaa agaagaagga aaaggaaatc aggaaagaac catgcggcaa
    11641 tggtttattc ttgcaggaac gataatagct gtagtcatac tgttagtgtt tatcggacga
    11701 ctaatctatc agtatagatt aagtaaaaaa gtgcttgatt tttcatttat tattagtgat
    11761 tcgtcaggga aaccggtaaa tggtagttca ttcattttga aacataagaa aaaaacactt
    11821 actagaaatg gtgtgccgtt ttcagtacaa agcgaccatc atgggaaagt caagctggat
    11881 caattgcctg gtggaactta tcgaatcgtt tcagctgatg atcctaaagt tgctgtttct
    11941 tttggtatta gaaaagtaaa acaagagaag atatactttt ttgaggggcg aaaacttgta
    12001 aaagagcttc aaaaaaatgg tttttatttt aaaatcaatg aataagtaag gagagaaaaa
    12061 atgatccgca caacaacaaa attgtctgcc aaagaactgg aatctgttct atcgatttgg
    12121 ctgcatgcta acttggattc tcatgaattc attccatcaa cttattggga gaatcaagta
    12181 gaatctgtta ggaaagcttt accagatgca gtgatttact tagcagaaat agatcaagaa
    12241 attattggtt ttgctggggt gattgatcag tttatcgcag gaatctttgt gaaaagggaa
    12301 taccggcaac aaagaatcgg tacgcaacta atgaaaaaaa taaaagaaac caataaaaca
    12361 ctgactttga cagtctatca aaaaaatcaa tcagctattc atttctatct ttcacaaggt
    12421 tttacaattc aaacacagca aaaagatgaa gcagtaaatg aggaggaact tgtgatgaaa
    12481 tggaatcaaa acgctgattt ttgatgaaca gaataacgaa ataatgctcc cagcctaatt
    12541 ttttgctggg agcattattt cattttgaag tcactaattt aactgtgtga atatgcagtt
    12601 gttacaatct ttgataaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg
    12661 gcaaaatatg aggaactatt tttccatttg tttctctaat actcaggtca aaacgctgat
    12721 ttcacaacct cttgataaac ggtgttcaaa cggctgcttc tcggtaataa gcccactagc
    12781 ctagaaaata tgaaaagcta ttttctaact agcgttctta ttactcaaag cataacgctg
    12841 atttcacaac ctcttgataa acggtgttca atcagctgac cctcggtatt agctcaaaaa
    12901 acggcaaaac atgaggaact atttttccgt ttctttctct aatactcagg tcagaacgct
    12961 gatttcacaa cctctctttc acaaccttat tttactcgtt tataattttc atcgaagtat
    13021 tttccgctac ggatatcatc tggcattcca tcgtatggtt cttcgctaat aacatcttca
    13081 ttgtctatac ctgcatcacc catataagtg atcgtagcaa attcaggaac aagtaatttt
    13141 ggataagttt catatttttc acgagctgct tccatttctg cgatatgttc gttttggaaa
    13201 gtaacagtga tttctggatg ttcttcaacc catttaggga aaaagatcat tccgtgaagt
    13261 ttcttttcat ttggcataaa gtccaatgcc acgtctgtcc ctgttggctc tgtccaagtg
    13321 attttgaata cgccgtccgt caatttaacg atatcagctt cttggtcttt tacccaacgg
    13381 cctttgacca ttccgccatg gatacggtaa tcaacagtat ggtcattttt ggcataccat
    13441 tcatattccc aaccattgtc atacgtatag ataaagtgag ttccaataaa atcttccaat
    13501 tctttaaatt ctttcatgtt taagcatctc attataaatt aaaacatgta ataaactaca
    13561 tgttttcgat tactcattta tgatagaatg gatttaaaga tttgtcaagg agggaaaact
    13621 gatggcaaga aaaaatacac ttcgatatat tttgttaggg ttattaagta agaagaagat
    13681 gacaggatat gaattgaatc aatcgttcaa aaatgaaata ggagaatttt ggcaagcaaa
    13741 acatagtcag atttatcctg aacttgcaaa aatggaagag caaggaatca ttcagcatca
    13801 agtagagatc acaggtgaaa aattggaaaa gaaagtttat gaattaacag aagaagggaa
    13861 ggaacaattg gcagagtgga ttcatacacc caccaatgaa ctaccggtta atcgagacga
    13921 atttgttctg aaattgtatt tcgtcaaaga cgtgaatgat ccggcattag tagaaattat
    13981 tcagcagcag cgtgaccttc atgaagaaaa acgacttcat ctattagagc gaaaaaaaac
    14041 gatctttcct actgaaaaag aaaaacatga aaattacgga cactacctga ttttagcgca
    14101 tgcgatcaat cgggaaacag aatatgcaaa ttggctaaca gatgtattag aagaaagtaa
    14161 ccgaaaaaaa taaaaaaata tccgaacagt gatggagaat tcctttctat atgaaagaaa
    14221 cgcagatcac cgttcggata ttcttatgtt caaacaggcc ttttttcaag tatttatgta
    14281 gcatctactt attaaaaagc ttggtactta ggagattagt ggcgtttaga gttcttttaa
    14341 caactgttcg tagtcagctt tttcatcgac aggaaggagc ttgtaagtta attcgtctgt
    14401 ctcgttgtca tgaatgatgt acatcttagc attctcttct ttgaagttac cgatttgtaa
    14461 aatcgtatag cgttcgtcga tcaattcgat atctttgatt gcttttgaaa aaactttacg
    14521 gacatggtgc attttctttt ggcggatttc tgccttttct gcagcttttg acaaagtaaa
    14581 tccgtcatct aaatattttt tgatcagttc tacttttatg actgtaggta aacgatattt
    14641 tctaggacaa ttcgcatcag cttcgatcga acgaatgaat cccttttgtt cccaatacct
    14701 taattgacgt ggagacacac ctacgatatc acttaattca ctgatgccaa caaccaattg
    14761 atcattttcc aacaaacgtt taagggaagc cccaaccatt tttgtcactc cttttgtcct
    14821 cttgcttccc ttaattatat ccgtattaac ttatgtgtca agtaggttga caaatagcga
    14881 atttaggact aaagtattct agtagtttca gaaaaaattc agaaagaagg agaaatatga
    14941 gtaacaacca acccgtagat atttacggaa aaccatataa tcggacactt ttagttgttg
    15001 tcttattaat tggtacattt tgtaccgttt tgaatcagac cttattgacg acagcttttc
    15061 ctacattgat gaaagctttc gatatttcag cgtctgatgt gcaatggtta acgactggtt
    15121 tcttgctagt caacgggatc atgatcccaa taacagcatg gctgatcaat aaattcagtt
    15181 caagaaattt atatctttct gccatgacga tctttttgat cggaacgatt acttgcttta
    15241 cagcacctaa ttttagtaca ttactaatcg gtcgtttgat ccaagcttgc ggtgtgggag
    15301 tatcgatgcc attattgcaa aatatcatgc tatctatttt cccgccagaa aaaagaggat
    15361 cagcaatggg gatgtcaggg atcgttattg gacttgctcc tgctttaggc cctacattat
    15421 ctggttatat cattgataac taccattggc gtgacttgtt tggtatggta ctgccaatcg
    15481 ttgttcttgt cttggtttta gcattcttcc taatgaaaag tgttattcca ttgtcaaatc
    15541 ctagcattga tattttatcc gctattttat caacgattgg attcggtagt ttactttacg
    15601 gattttctag tgtaggagat gacggctggg gaagcttgaa agtaattggt ttcttagttg
    15661 ttggtgtgat tttcatcgcc ttgttctcat ggcgtcaatt acatttggaa catccattct
    15721 tggaacttcg tgtattcaaa tcatctacat tcacgattgc agctgtttta gctggtgtaa
    15781 caaatatggc gatggtaggg gcagaaatgg tcgtaccatt gtatattcaa aatattcgtg
    15841 gagaatcagc attccattct ggcttgatgt tgttgccggg tgcattgatc atgggggcta
    15901 tgatgcctgt gacgggtgcg attttcgata aatacggcgc gaaacgttta gccatcacag
    15961 ggatgttcat tttgacagcg gcaactgcac catttgcttt cttgacaaaa acgactccag
    16021 ttttatatat cgtgatcctt tatgcgatcc gtatgtttgg gatctccatg gtcatgatgc
    16081 cggtaacgac ttctggtatg aatgcattgc caaataactt gatcagtcat gggacagcgg
    16141 taaataatac attccgtcaa atcgcaagct cgattggtac agctattttg atcagtgtgt
    16201 tgacgaatgt gacaaaagca aatatcccaa gcaaatctgt attggataat acgccattag
    16261 cttataaaga tcaggcaatc aatgccacac tgtcaggtta ccatgccgcg tttatcgtag
    16321 cgatcatctt tgggattctt ggctttatta ttgccttttt attgaagaaa aaagaacctg
    16381 ttctaatgaa agcgggggat aatgcatgat tattggagtt ttgattgtca gcttgcttgc
    16441 ttttgcttta acgaacgtgt tcgctaaaaa aacatggcaa acctttttat ctttgatttt
    16501 cggactaatc ttcgtcgctt cactaagttt gatcgtcgct aatttgtcca atcactttgg
    16561 aatggaaaaa gtaacggaaa caaaaacaga aaaaattgtt tccagtgctg attcccaagg
    16621 agcagatatg cttttgtata aagctttagg gaatggaaaa gaaaaagtgt atctttaccg
    16681 aacaaatgaa aaacaagaaa aaccaaaagc aactggtacg gataacgaaa caaataaagt
    16741 agaaaagaca gacggcgatg ctgaaaaagt gacgaaaact acttattggg aatacaaaaa
    16801 cgacatgtat aaattctggt tcaatattgc tgataacaat cacgaatatg acagccgagt
    16861 gaatacattc aaaatcccag agacatgggt agaattaagt acggaccaag cagccaagtt
    16921 agctgaatta gtgaagaaac agcaaagtac gatggaaagt gaagcaaaag cttatgttca
    16981 agacggtatg gtaaaagcaa tgactgaaaa tccaaaaatg agcaaagcag agcaagaaca
    17041 acgtacgaaa gccttagctg ctgaattcca acaacaagca ttcgcaaaat tagtgaaaga
    17101 agcaaaaggc gaataatatt ttgaacacca tgta
//
LOCUS       DACCCS010000056        17107 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.057, whole genome shotgun sequence.
ACCESSION   DACCCS010000056 DACCCS010000000
VERSION     DACCCS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.057"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            7..1398
                     /locus_tag="IVK82_002076"
     CDS             7..1398
                     /locus_tag="IVK82_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP6393122.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1414..2454
                     /locus_tag="IVK82_002077"
     CDS             1414..2454
                     /locus_tag="IVK82_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6393123.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2473..3558
                     /locus_tag="IVK82_002078"
     CDS             2473..3558
                     /locus_tag="IVK82_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP6393124.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3591..4553
                     /locus_tag="IVK82_002079"
     CDS             3591..4553
                     /locus_tag="IVK82_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP6393125.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            4546..5979
                     /locus_tag="IVK82_002080"
     CDS             4546..5979
                     /locus_tag="IVK82_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP6393126.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKGELI"
     gene            5976..7046
                     /locus_tag="IVK82_002081"
     CDS             5976..7046
                     /locus_tag="IVK82_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393127.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            7033..8454
                     /locus_tag="IVK82_002082"
     CDS             7033..8454
                     /locus_tag="IVK82_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP6393128.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            8467..9474
                     /locus_tag="IVK82_002083"
     CDS             8467..9474
                     /locus_tag="IVK82_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6393129.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            9486..10490
                     /gene="neuB"
                     /locus_tag="IVK82_002084"
     CDS             9486..10490
                     /gene="neuB"
                     /locus_tag="IVK82_002084"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP6393130.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            10487..11635
                     /gene="neuC"
                     /locus_tag="IVK82_002085"
     CDS             10487..11635
                     /gene="neuC"
                     /locus_tag="IVK82_002085"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP6393131.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            11608..12285
                     /locus_tag="IVK82_002086"
     CDS             11608..12285
                     /locus_tag="IVK82_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP6393132.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            12275..13417
                     /locus_tag="IVK82_002087"
     CDS             12275..13417
                     /locus_tag="IVK82_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP6393133.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            13430..14407
                     /locus_tag="IVK82_002088"
     CDS             13430..14407
                     /locus_tag="IVK82_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6393134.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            14407..15315
                     /locus_tag="IVK82_002089"
     CDS             14407..15315
                     /locus_tag="IVK82_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP6393135.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            15316..16068
                     /locus_tag="IVK82_002090"
     CDS             15316..16068
                     /locus_tag="IVK82_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP6393136.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            16073..17020
                     /locus_tag="IVK82_002091"
     CDS             16073..17020
                     /locus_tag="IVK82_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP6393137.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         6164 a         2190 c         3090 g         5663 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaaacat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
      961 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
     1021 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
     1081 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
     1141 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
     1201 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
     1261 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
     1321 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
     1381 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
     1441 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
     1501 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
     1561 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
     1621 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
     1681 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
     1741 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
     1801 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
     1861 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
     1921 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
     1981 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
     2041 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
     2101 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
     2161 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
     2221 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
     2281 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
     2341 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
     2401 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
     2461 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
     2521 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
     2581 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
     2641 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
     2701 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
     2761 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
     2821 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
     2881 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
     2941 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
     3001 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
     3061 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
     3121 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
     3181 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
     3241 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
     3301 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
     3361 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
     3421 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
     3481 ataacacaag aactaaagag ttatgatgtt tcgtacacta ctgaacaaat ggagagtata
     3541 tatagtagag atcattaaaa atgttcaaga tattttagga gtgattattt ttgaaaatat
     3601 gttatttgat attggcatac aatgatattg gcaacttaaa gagattggtc aatcgattga
     3661 acgaaaatgc agtttttatt attcatatag ataaaaaaac tgatatagaa ccatttattg
     3721 aagcatttaa atcatatgat aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt
     3781 attggggtgg tttttccatt gtacaagcag aaatcaattt agtaaagaga gcattacaaa
     3841 atgagaaata tttaaaatat gtattattat ctggtgctga ttatccaatc aaagataatg
     3901 agtatattta taattatttt aaaaagaata gttcagtgga gtttattcgt ggaatagatc
     3961 tagatcaaat taaacataaa gaattttatt ataaacatat agatgtatat caaaaacatg
     4021 attatcctag aattaatagg aataacacaa ctgcatttaa aatttttaga gctataatta
     4081 atcgttgtct gaggatgatt aaacttcccc caaaaattag acaccataaa tttgatttgt
     4141 atcatggttc tcaatggtgg gctttgtcga aagaatgcct aacagaatta atacaaatgt
     4201 atgaacaaaa tcaacaagac tacctgaatt ttaaaatagg tatgtttgcc ccagatgaaa
     4261 aattttttca tacacttttt tttaattcct cgttcaaaaa taaaaatgtt attggaggac
     4321 cagatttacc acttgaattg aaaaatattg aagaaactac tttacaaact tcgaaattag
     4381 caaatattca tattattgat ccttccatga acaaatggtt tgatgaaagg gatatagatc
     4441 aaataaaaga aagtgaaaaa ttatttgtaa gaaaagttaa atcggattac tctgataaac
     4501 tattaaatag aattgataaa gaaattttga aagttggaaa tatagatgaa tagaatagta
     4561 cctgttttag agaagagttt attaagtcga gatgcgtttc gagtaataaa tttaatcatt
     4621 tgtctgcttt attttatgcc tagtactttt aatgtaacta atatcccgat gaaaatagct
     4681 tttgcttggg gtggaattat agttttgtat gaggttttta taaagaaaaa tttttttaaa
     4741 atgcgttttt cttggctact gattggagca atgattagtt ttatcttaac tataatcatt
     4801 aacagagaaa attttttaat tccatctatt tataattttg gatatttgct gattactttg
     4861 atagtaattt ttcctgcaga cttttcacaa agtgaagagg aaaaaaagaa aaaaatggtg
     4921 tattttaata atgttttcat tatcataatt tttttagctg cgctaatatc tatctatcaa
     4981 tttatttttt tgatttctta tcatgtccca acaggaacgc caggattact tgcaagacaa
     5041 ggttttattg aacatcggtt atttggtgtc tatacaagtc caaatgttgg agctatgttt
     5101 ggatatactt ctattatttt aagtttaatt agtgtattaa tttcaaaaat aacaaaaacg
     5161 attcgtgttt tttatatgat gaattttatc gtacaaatat tttatattac actttcttcg
     5221 tctagaggtg cccaggtagt aatttgttct tttttagtat tatttctttt gttgtttgcc
     5281 aattcaaaat ttagaaagag cattagagaa gtactacctg taaaaatcaa atggatggta
     5341 agcatattat tagttctatt ggtatatttt tcttttggta ctaattatgc taaggatttt
     5401 ttagcaacaa ttcctgtatc gattgataga aaagtagaag ttatagagtc aaatgaatca
     5461 gataaaactc cacttccaga aaaagaagta atattacagc attctagtga taactccgaa
     5521 atttcagctg gtcgttttac gatttggaaa gctgcgctta aagttataag accaactttg
     5581 ttatttggtt atggagagac cgatttctat gggaatagcg atgcaaaatt tttgaaaaaa
     5641 gtagatcttg atactacaga tgtgaatgaa ctaaaaagag cacatggaaa catgcataat
     5701 ggatttcttc aagtacttgt atcaagtggt ataattgctt ttatatgtat ctatgctttc
     5761 tatgtgttaa atatctttag tttactcaaa acatttttta gaagtaaagt gaattatgcg
     5821 tttcttggta ttgttttaat ttttatacta tctattttta tagatgaaat ggtggaggct
     5881 catgtacttt tcaataagcg cgatgtcatt tcaattgttt tctggtatta tttgggaagc
     5941 atttcttact tgtatttaaa aaaaggagaa ttaatatgaa gacagctttc attgcttgga
     6001 ctcctttaca tttgattaat atattaaatg tttatcaaaa tttttaccga gaatcaaaag
     6061 gagatttatt tatttattct gaatttgaaa acgctagaag atatagtgag cgtataaaag
     6121 aattagggct atttaatgat gttgtttttg tgaatcaccg gaaaatcggt tcttccttta
     6181 aacgtaaaat taacttgtta actaaccata atcaattttt ctcggatgat ttatccgttt
     6241 atgatgaaat ttttatacag ggggaaaatt atttcacaaa aatacttttc tccaatgttt
     6301 acaaaaaaaa tccagatgta aaactaaatt atatagaaga tggtttgggt acttatttga
     6361 agaagagtat attttcaaca agtaaaaacg ttcaaagagt ttttaaaata ttaaataaaa
     6421 atagcatctt caaaaaaaaa attgacgatt attttgtcta ttgtccggaa ttgttacttt
     6481 atgatgtaaa aaatataaaa aaaattccca aaatagatga aaacagtaaa ttgatgaaaa
     6541 acatacaatc aatctttggg atagaagaag tttttgtcgg agataaatgt atcttttttg
     6601 atcaaccttt attatctgat ggttatggca tagatgaaac agatattttc gctaaagtaa
     6661 atagtatatt ttctggaaaa aaagaaactt taattaagct tcatcctaga gcaaggacaa
     6721 atcgttatgg tgaaaaaaac atattgaatt ccagtttacc gtttgaagtc ttgctacttg
     6781 aaaatgactt ctcttcaaca cttttgatta gtcctatatc tacaatttct ttttcaccat
     6841 ctatgatgtt tgaaaagacg atcaactcta ttatgttggc taaattaatt ttaaatgaat
     6901 atacagcatt atctgaagat aaaaaagaaa cattgacaaa tattgttctt ttctgtgaac
     6961 attataaccg tctatcagaa gaaaaaattt tactcccaga aaactgggca gaattaagaa
     7021 agttggtaga gtatgggatt atttaacaca ctttccaagt caaaattagt taagtccagt
     7081 ttctggtata ctatagggaa tttttttatt aagggaattt cattcataac tattcctatt
     7141 tttgtaaata ttactactca agcagaatat ggtttgatta ataatttcac ttcatttgta
     7201 tcaatttttt cgattcttgt tgggctaagc ctaaattcgg caattaataa tgcaagtttt
     7261 gactataaag atgacataaa tgaatttatg tcatcggtac tttctctagc tacactttcg
     7321 ttaattgtgt tcttattaat agggaacata tattttttgt tttcaaatgc tttttttgat
     7381 atgaaccaac ttgtatttaa tttgatgtta ttacaaagtt ttgctagttt tgtaattagt
     7441 tatatccagt cttattttac gattaacttt aaccatttta aatatttgat tttatctttt
     7501 atatctactt ttttgaatat tgcaatctct gtagctttga tgcagacatt atattttgat
     7561 gataaatatg ttggcagagt agtcggcgga acactgtcat ttgtgttgat aggtttagta
     7621 atttatttaa gaataatgat aaagggtgct catttttttg ataaaaaaca gtggagatac
     7681 gctttaaaaa tctctttgcc gctaatccca cattccttag caaatatagt tttagcacaa
     7741 ttcgatagaa ttatgattaa ttcttattta ggtcctgctg cggggggaat atattcctac
     7801 attagtaaca taggtattat tctaagcgta atatggatgt ctactaataa cgcctgggtt
     7861 ccatggttct atggtgagat gagtaaaaat aatgaaaaaa aagtgaagaa agttagtaac
     7921 tattatttag tagtctttac tttgattacg atgattgtaa tggtagtttc gattgattta
     7981 gcaaaattta tggctccgaa agactattat tctgggttat ctttagtggt tccgataacc
     8041 ttaggctatt tctttcagtt tttatatagt ttaccagtta atgctgaatt ttatgagaaa
     8101 aaaacatcgt ttattgcgat tggtacagta gcaagtgcta tagttaatat actattaaac
     8161 atgattttca tacctaggtt aggaataatt gctgctggat acacaaccgt atttacgtat
     8221 tttttacttt ttatttttca ttataatatc gctaaaaaaa ttacgaataa acaattattt
     8281 gacacaaaaa aaatatgtat cataataatt tttgtatcaa ttttttcttt gatacttcat
     8341 attttaatca atcaaatgtt gattcgttac gttttgacat cattattgat aattagttta
     8401 gttatatatt ttttaaagat taagaatcga attatagatg actataattc ataggaggag
     8461 atataagtgt ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt
     8521 gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt
     8581 gaagaaaaac aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta
     8641 attggtgata tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt
     8701 tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta
     8761 aaaacaaatg tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa
     8821 cattttgtag gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca
     8881 aagttattga gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca
     8941 atattttctt gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt
     9001 tttagtaaac aaataaaaaa caataaagat attactttga cagatgttga tatgacacga
     9061 tttatgatgt caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa
     9121 tttggagaaa catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca
     9181 ttaataaaaa tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta
     9241 ggtatcaggc caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc
     9301 tcatatgaaa atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg
     9361 tacaaagatt atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg
     9421 agtggacagg aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt
     9481 ttatgatgaa atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc
     9541 tagatttagc tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat
     9601 ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga
     9661 aggataatct taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc
     9721 aatctgattt tattagaata aaagattatt gtatacaaaa taaaattgat tttttgtcta
     9781 ctccttttga tgaagaaagt gcagactttc taaaacaaat tggtgtacaa ggatttaaaa
     9841 ttggctcagg agatttaaca aatttacctt ttcttagaaa aatagatatg tatgggttgc
     9901 cagttttatt atctacaggt atggctgata ttcatgaggt cagtgaggct gtagattgtt
     9961 tcatagatag tccagttact attttacatt gtacttcaaa ttatccggct agttctgaag
    10021 atattaattt attggcgatt aacacattaa aagaaatgtt cgggataccc gttgggtact
    10081 ccgatcattc attgggatat gatgttggta tttgtgctgt tgcgatgggt gctaaggtaa
    10141 ttgagaaaca ttttaccttg gataaggaac tacctggtcc agatcataaa gcttctttag
    10201 atcctcagga atttgcagat tttgtaaagc atattagaaa cactgaaata tttttaggtg
    10261 atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa agaagtatca agaaaaagcc
    10321 tagtggtcac tgagaattta aatgtgggag atattcttac agagaaatct cttacaataa
    10381 aacgtcctgg aaatggaata gctcctaagt ttttatatca atatattggc aaaaaagtta
    10441 aaagaccaat aagtgcagat agtgttctgc ttgaggaaga tgttgaatga aaaaaatact
    10501 tatagtaagt ggaacacgag ctgattatgg aatatataaa cctgttgctg agaatttact
    10561 acaagataat tgggatgtat catttgttgt ttctggaatg catttatccc ataaatatgg
    10621 atttactaaa gaattgatct tagctgacgg atttagaatt agtggagaag ttcaaagttt
    10681 gtttttagag aacaataagg gaaacatggc tcggtctgtg gcattagaga taaatggttt
    10741 cacaaatata tttgaacaaa ctagccctga ttttgtatta ttactagggg atagaggaga
    10801 aatgttggca gctgcgatag cttgtttgta tcttggaatc aaaacagcac atattcacgg
    10861 aggcgaagtg tctggtactg tagatgaatc aataagacat gccataacga aaatagcatc
    10921 tgttcatttt gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg gggaagattc
    10981 ttggagagtt tttcccgtcg gagctcctag aatagatact attttaaata atagattacc
    11041 tagttttaga gatataaaag aaaaatatca tctaaaattt gaaaagaaaa aatacgtttt
    11101 gcaagtattt catccagttg taaccgaact agaaaatata gaaaaacaaa ttttagcttt
    11161 gatcaattca atggaacaac aatctcaacc aattatttgc attttaccta atagtgatgc
    11221 tggtagcgaa attatcagaa aaatgtacca gaaatattta aaaacggata ttatttatat
    11281 tacgaattta gagccggagg aatacctgac tatattaaaa gagtgcaaat ttatgatcgg
    11341 aaattcttct tctgggataa ttgaagctgc atcattcaaa ataccagtaa taaatttagg
    11401 aaccagacaa aatggacgag aacaatctaa aaatattata aatgcaaatc taaatgaaaa
    11461 tgaaattgaa actgctttga ataaaataca agatcctcag tttataaaaa gcttgaaaaa
    11521 tgttaagaac atctacggtg acggcaacag ttctagaaga ataacaaaaa cattgaatca
    11581 aatattgaat gatggggaag atattaagtg gattcaaaaa caaatagctt attaaaatat
    11641 actagtgatc catacattat tattggggct ggcggacacg caaaagtcat tattgaaacc
    11701 ttaagattag aaaataagtt aatatatgga ttttttgatg ataagcaaaa taaaatgcaa
    11761 gatctgaaat atcttgggaa aataaacgaa ttgaatagct gggcggatag caaaaaaaaa
    11821 atctataaat ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat tataaaaaaa
    11881 ttgaatatgc caagtgagca atatggaata gctgttcatc caagtgctat agtggcaaaa
    11941 gaaatttcaa ttggttatgg aactgttatt tttgcgaatg ctgtaattaa cccagatgct
    12001 gtaattggag agcatgctat aataaacaca ggtagcattg ttgagcatga ttgtagaatt
    12061 aataactatg ttcatttatc tccaggtgta tgcttagcgg gtggagtaca tgtaggtgta
    12121 ggaactcaaa taggaattgg ttctcaatgt atccagatga agaaaattgg atcttggtgt
    12181 atgataggcg cagggagcac tattgttaag gatattccct cacattcctt agcttatggc
    12241 aatccagcta aaattaagaa agaaggaatt aattttgaat tctagaattt tattatcaag
    12301 tcctcatatg ggaggaacag aagaaaaata tgttgctaag gcttttaaag ataattggat
    12361 agcgccatta ggtgaaaatg tggataaatt tgaagaaagt attacaaaat atactggttc
    12421 taagggcgga gcttctgcgt taagttcagg tacagctgcc atacatcttg ccttaagact
    12481 gcttaatgtt ggaaaaaacg atattgtctt tgctccttca tttactttta tagctacagt
    12541 aaatcctatt ttatatcaag gggctgtacc agtgttcatt gactcagaac ctaatacatg
    12601 gaatatgagc cctgttgcac tagagagcgc attagaagaa gctgaaagaa acaaccaatt
    12661 acctaaggca atcatcgtag tgaatttata tggtcaaatg gctgattatg aacgaatttt
    12721 agctattgca aaccaattta atgttccagt gatagaagat gcagcggaat cgttaggttc
    12781 tacttataag ggaatttcgt caggcttatt tgggacaatt gggatctatt catttaacgg
    12841 taataagatc atcacaacct caggtggagg agctctagtg tctagtgata aaaaatatgt
    12901 tgagcattca agatttttag caacacaagc aagagatcaa gcaccgtatt atcaacatag
    12961 cgaaataggt tataattacc gtctaagcaa tatttctgcc ggtatcggac gtggacaaat
    13021 ggaagtattg aatgatagag tagctaaacg aagagaaatt ttcaatcgat ataaggaagc
    13081 tttttctgga attgatagaa ttgaaatgat gccagaagtg gatggcatgg tgccaaatcg
    13141 ttggttgtct actttaacga tcaatgatta taattctatt gatcaagtag ttaacgtaat
    13201 aaataaacta aatgaaaaaa acattgaaac aagagctttg tggaaaccac tacatttgca
    13261 accagtattt caaggggtaa aaatgtatta ccatgataat aatcttagtg tgtcagaaaa
    13321 gttatttaat agcggcttat gcttaccttc tggaagtaat atgacagata gtgaacaaga
    13381 atttgttatt gatgagttta aaaaaatatt caagtaggtg tgatatttta tgaacatagg
    13441 aattattggt ttaggttcca tggggaaaag aagacttaga ctcattcaag aacactttaa
    13501 tgatatttta ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag ttgaaaagga
    13561 atttggaata ccaacttata atagtctaac agaggcaaaa aatttgtttg aaataaattc
    13621 agtttttgtt tgtacatccc ctataaccca tgaatttatt ataaaagagg ctttaaattt
    13681 agattgtaat atctttacgg aaatcaattt attaaatgaa tattatgatg aaataatcga
    13741 agtagctaaa aagaaagata gattacttta tctatcttct acatttttga agagaaaaga
    13801 aattcaatat attaagtcta aagtgacgca taataaaaaa atttcttatc aataccatgt
    13861 aggacagtat ttacccgact ggcatccttg ggaagattat cgcaattttt ttgtttcaaa
    13921 caaaaaaact aatgggtgta gagaaatatt cgctattgaa ttaccttgga ttatagatac
    13981 atttgggaaa gttaaatcgt ttactgtaat gacccagaaa ttatccaatc ttgatattga
    14041 ttattcggac agtttcactc ttattattga gcatgaaact ggtatagttg gaactttaag
    14101 tgtagatgtt gtttcaagag tagctaaaag aaatttaact ataattggtg aaaagatttt
    14161 aatagaatgg gaaggtaacc cggattctct attttcatgg aatgaagaaa cttctagtat
    14221 gacagatatt caattgtacg atgaggttat gcatgataat aattattcac atactattat
    14281 tgaagatgca tatttagagg agattaaaga atttttcacg cttttggaaa agaaaatgga
    14341 accccaatat acttttgaaa aagataagta tattattaac ttaattaacg acattgaggt
    14401 aaattaatga tgaaagtaat tttttttggg ttagggtcaa ttggaaagag acatctaaaa
    14461 aatctaataa gctatggaaa aaagaattct attaattttg atataacagc ttatagtagc
    14521 agtggaaaaa aagagtttga agaaataacg tatattcaaa atagagacga tttggataca
    14581 cattatgata tagcttttat tacaaatccg acttttaagc atctggaaac actaaatgta
    14641 attgttgaaa aagttgatta catattttta gaaaaacctg ctttttctga gagtattgat
    14701 cttactcaat ataattatga ttacaaaaat atttatgttg ctgctccact gaggtataaa
    14761 aatatcatgg aagttgttcg taatagtatt caaagtaaaa aagtatattc tgtaagagct
    14821 atttgttcta cttatcttcc tgattggaga aaaggtactg attatagaaa taattattct
    14881 gcctttaaat ctatgggggg aggagtagaa ttagactgta tacatgaatt ggattatatt
    14941 ataaacctat ttggtttccc taaatcagta aaaaaacagt ttggtaaata ttcgaatctt
    15001 gaaattaaca gtaatgattt agcaacatat atattgagat acgatgataa aattatagaa
    15061 attcatttag attattttgg aagagttcct acaagaaaat ttcaattgtt tactttagat
    15121 gaatgtatca atgttgactt attaaataat aaaataagtt ttgaagaaaa cattgttaaa
    15181 gaaatgcctg aatctgctaa tgatatgtat gagaaggagt tgaaatattt ctttgaaaac
    15241 gtagtaacag gcaaagaaaa ctggaatgat ttatatcatg caaacgaagt attgaaaata
    15301 gctgaggaga aataaatgga aattttattt actatttgtg gtagagctgg gtctaagggg
    15361 tttaaaaata aaaatttgaa atatttggca ggaaagcccc tagtccattt cgctttagca
    15421 atgatagata tctatcaaca aaaacatcct gaacataatt gcacgacttg tttgaatacg
    15481 gacagtaatg agttagtaga attgacgact aagttaaatc cgtctgtgaa aattgtagaa
    15541 cgtaaacctg aattagctac agatactgta gggaaaattg aagtgatacg tgattcattc
    15601 ttacaactag aaagagaaga taatatattt gatttaatta tagatttaga tttgacttca
    15661 cctttacgtg aagtttctaa tcttgaagaa ttaatagatg aacatctaaa aaatttagat
    15721 aaagacgtct acttttcagt tgtttctgca agacgtaatc cctattttaa tatggtaaaa
    15781 agaattgaaa atgaagttac tatagttaat aaatcaagat acacatctag gcaacagaca
    15841 cctgaagttt ttgaaatgaa tgcctcaatg tattctttta aacctgaatt tctaaaaaaa
    15901 agtaattata tttttgatgg gcaatgtggg ataataaaaa tgagagatta tctaatttta
    15961 gatattgata gtgaagaaga ttttaaatgg ttagaatttt tgtttcctaa atttttagaa
    16021 gattcttcgg agcttcaaga tatctataat tatttatatc ggaggtaaaa ggatgagctg
    16081 ttatcttatc accggtgggg ctggcttcat tggttctacc ttagccaatc atttaggaga
    16141 agaacatgtg gtaatcgtcg tcgatgactt atcaatggga aaagtagaaa atcttgatat
    16201 gacgaaaaat atcactttta ttgagggaga tgtcgctgag aaatccttga tggaagagat
    16261 catgaaagct aataaatttg attatatctt tcatttagca gcagttgcta gtgtggcaga
    16321 ttcagttgcc cgaccagtag aaacacatcg tgtgaatttt gaaagtgtat taatgatttt
    16381 agaattgatc cgcaagtatc aacctgattt aaaacggatc gtgtttagtt cttcagcggc
    16441 tgtttatggg gatgaaccga ctttaccaaa aaaagaagag tccgtgattc gcccattgac
    16501 gccttatgcg gttgataaat tcgctgcaga acaatatgtg ctagattatt gtcatttata
    16561 tgatgtaccc ggtagcgcag ttcgtttttt caatgtctat ggtccaaatc aaaatccgaa
    16621 ttctccatac agtggtgtga tttctattct cgtagatcgc tataagaaac aaatcgtagg
    16681 agaagcaact tcatttacat tatttggtga tggaagccag tctagagact ttgtttatat
    16741 agatgatgta atccaagcat tacttttagt tgcgaatgag gaagctgcac tggggaaaca
    16801 attcaatgtt ggaacgggta aagcaaccac acttttggaa ttgattcatg caatcaatga
    16861 aattctcggt gtagaattga cacttgaata caaagaagag cgttcgggtg atatccatga
    16921 ctctctagca gatattacta agatccagtc aattggctat caaccgaaat atggtatttt
    16981 aagtggaatg actaattatt tgaaaactga aatcaattaa aacatactaa gataagatta
    17041 gagggtaaat tcaggctctt tgtcaataag gactgatgag ttttgtaaaa tcaaatctgg
    17101 gatagaa
//
LOCUS       DACCCS010000057        17030 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.058, whole genome shotgun sequence.
ACCESSION   DACCCS010000057 DACCCS010000000
VERSION     DACCCS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.058"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..146
                     /locus_tag="IVK82_002092"
     CDS             <1..146
                     /locus_tag="IVK82_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP6393138.1"
                     /translation="FCVPDSFSNYRISREVGYSERSIQRMKSEALIEFAEAYKHGRII
                     AYK"
     gene            159..545
                     /locus_tag="IVK82_002093"
     CDS             159..545
                     /locus_tag="IVK82_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393139.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            1095..1319
                     /locus_tag="IVK82_002094"
     CDS             1095..1319
                     /locus_tag="IVK82_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393140.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            1337..1765
                     /locus_tag="IVK82_002095"
     CDS             1337..1765
                     /locus_tag="IVK82_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393141.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            1743..3161
                     /gene="terL"
                     /locus_tag="IVK82_002096"
     CDS             1743..3161
                     /gene="terL"
                     /locus_tag="IVK82_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HAP6393142.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            3173..4708
                     /locus_tag="IVK82_002097"
     CDS             3173..4708
                     /locus_tag="IVK82_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP6393143.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            4788..5666
                     /locus_tag="IVK82_002098"
     CDS             4788..5666
                     /locus_tag="IVK82_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP6393144.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5668..5985
                     /locus_tag="IVK82_002099"
     CDS             5668..5985
                     /locus_tag="IVK82_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393145.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            6034..6735
                     /locus_tag="IVK82_002100"
     CDS             6034..6735
                     /locus_tag="IVK82_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP6393146.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMIMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            6750..7640
                     /locus_tag="IVK82_002101"
     CDS             6750..7640
                     /locus_tag="IVK82_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAP6393147.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7663..7878
                     /locus_tag="IVK82_002102"
     CDS             7663..7878
                     /locus_tag="IVK82_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393148.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            7890..8231
                     /locus_tag="IVK82_002103"
     CDS             7890..8231
                     /locus_tag="IVK82_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393149.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            8231..8602
                     /locus_tag="IVK82_002104"
     CDS             8231..8602
                     /locus_tag="IVK82_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393150.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8595..8990
                     /locus_tag="IVK82_002105"
     CDS             8595..8990
                     /locus_tag="IVK82_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP6393151.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            8992..9369
                     /locus_tag="IVK82_002106"
     CDS             8992..9369
                     /locus_tag="IVK82_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393152.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            9370..9978
                     /locus_tag="IVK82_002107"
     CDS             9370..9978
                     /locus_tag="IVK82_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6393153.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            9978..10319
                     /locus_tag="IVK82_002108"
     CDS             9978..10319
                     /locus_tag="IVK82_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393154.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            10561..13350
                     /locus_tag="IVK82_002109"
     CDS             10561..13350
                     /locus_tag="IVK82_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP6393155.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSKAINRVKEIFDSLREINLFEIGKNIIDGLINGVVEKWNALKKTI
                     KGIAGSIKDSIKGALGIHSPSRWMRDMVGKNIVQGIIVGIDKEKSKLDQTMTDLVKTP
                     SVQPVITGSNSQPVVQAKQNTSSNAVNEIHLHLNVYGDLPDSMIKQIAKKMKTELTRQ
                     MKRDADAVGGTLYAT"
     gene            13340..14080
                     /locus_tag="IVK82_002110"
     CDS             13340..14080
                     /locus_tag="IVK82_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6393156.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKAYYKNVTRKIECYYKAPSIDLVQEWEDRITEWLDMNSYSDFILYYDQQYIYQ
                     AIVTEAPEFKGTRKTGNIVPFEFTVSLRPFKENYSGRFAIQQIKAFELFNPEKYASKP
                     LIKLSGSGDASFYINNDKYDLKLLDRELYIDSKLEESYRKLDDNLEHQDHVTLFLDFP
                     FLYPGKNEIKWTNNIHSFEIIPRWWRKV"
     gene            14077..16788
                     /locus_tag="IVK82_002111"
     CDS             14077..16788
                     /locus_tag="IVK82_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP6393157.1"
                     /translation="MIPRIYSPTETDFSTNGLGILKDTTKCEIYEVANGKYELELEYP
                     LGTRFDEYFENDYQIKAKSNDQEEYHIFFIDDKDIDTFLDTVTIYAQSRTNRLGRRAV
                     TFAGVDSKTGREAMAIIENNMDKKSDIRLYSDITTVSSTTFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRDNIGTIRYGKDLSGLKVKLDWTGVKTRIIPYADPQS
                     DAGTTSRIYGSPVDSEYINNYPDVYTEHVQFTEEQGVKDVNSLNKIAKNYFKTINPGC
                     DKPKISITVEFDKLTDTEEGKEFAKIRNYGLFDTFKIYHRKYKLYFESKVSGVQYDSL
                     SEKVLKLEAGDAQVAFYQQQAVTIQDKLKDYATNNYMSSFNDYVSSMIAGQGNAGGYV
                     VLWPKEKPSNIFIMDSPDLNKAKEVLRMNKNGIAFSKNGWNGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSNSSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVNGKEITGNNSGGSDPGTIPPQLTTEAEKRAWKIWTMLKARGYSEYAAAG
                     ILGNIQGEVGASMNPDTEQLGGPAYGIVQWDGSAYPLVGSPTWNGREYVQRLMNTAGI
                     QEDYRSIEAQVKLLDWCMFNGQWLGKVNPTTVSGFKSINDAKSAAYAFEMNFERPASA
                     HPERQNYAQSWYNKLHGLTSPEPGGNFICPIQKPVTVTSECGWRTSPINGGQEFHNGI
                     DLVNGNPNTPVFAALDGEVVQAGANYYDWYGNYVVIKHNNGKWTGYAHLSRIDVSVGQ
                     KVQKGAQIGLMGTTGPSTGEHLHFQIMKNYWPQPVVDFENPRNYIQF"
     gene            16801..>17030
                     /locus_tag="IVK82_002112"
     CDS             16801..>17030
                     /locus_tag="IVK82_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP6393158.1"
                     /translation="MSKWNVVLSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKI"
BASE COUNT         6069 a         2416 c         3576 g         4969 t
ORIGIN      
        1 gcttctgtgt gccagatagc ttctcaaact acaggattag ccgtgaagtg ggttattcag
       61 aaagaagcat acaaaggatg aagtcggaag ctctaataga gtttgcagaa gcgtataaac
      121 acggaagaat aattgcttat aaataggggg accatataat gtggaaagat tatgtatcgt
      181 tgaaagagtt gaaaaaagat cttgttttca aaagaattgt agaatggtca gagagtgaat
      241 taattttaga agatggaacc aaaatggaag ttgtatgtag cgaatcagat tgctgcgcat
      301 gggccgaagg tgaattcaaa aatgtaaagc ttgatgcagt gattacagat ataaagattt
      361 ttgataaagg taaccgtctc tataatggtg acggacatac ctcttatgct gaagtcgttg
      421 tttatcataa tcgtaatgag atcgctaaag cagaatgcac agcgaatgat gggaatggcg
      481 gctactatta tagtgtttgt gctttaaagg tcaaagacaa actttgtata gtgactgacg
      541 cataaaaaat ggcggttttt tggcggaatg atggcggttt ttagccattt atcagtgata
      601 ttatggtagt gtcgaaagat tagtgatagg tctgagacaa aataataata aaaggaacat
      661 cgttttatta ttgtttcaca attaagcttc gatagacagc aacggaaata ttaagaataa
      721 ggatgtgaat tttaactcct tctaaattgt tcttattatc tatcatccgt tgctgtctat
      781 tgttacatac agatcgctta ggcggtcttt ttattttggc cgaaaaccta cattttcgat
      841 ggccgattat tggaggagga atagcatgtt caaactatct gaaatcatta agaagtccga
      901 cgttgagaaa ttagagatgt taaaaaagaa gttgaagaaa tagcagtctc ttcgtgaggc
      961 tgttttattt tgctcacaaa aatagaccac tatcgggtaa tagtggtcag gaattaaatg
     1021 aaaaagatgt tagggttgtt agctaagtat aacatcataa cgcttacaaa acaatgcaag
     1081 aaggataaat tattatgaga acttactggt atgtgtcatt aaacaataaa tatccgaagc
     1141 caatgaaagg acagcatagg cgtgtagtca tgtctgttca aatgaaggcg aagtattcga
     1201 ttgtagaaat gatcagagag gcaacgccag tagagattga ttattgcaag ctagtctatt
     1261 gtgggtgcgg ccgttggaaa gaggatcatg tacaaaaaaa tatcagcaag tacatataga
     1321 gaggcggtga ttaaggatgg aaaaaagcga tacgaaagcg aacagataca aacctactgc
     1381 tgcagagaaa aaactcttgg aagtattgat aaatccagat aacttaggca aaccggtgca
     1441 agagttgtgt tcactggcga aaataggtag gaccaaatat tacgacgcca tgagcaaaga
     1501 tggctttgtt gatctagtta acgaaacaac aatggatctg atcaaaggaa aagcttctga
     1561 tgtccttaat gccacttaca agtatgcatt gactgaaaaa ggtcatcagg ataggaaaat
     1621 gattctgact attgctggaa tctatgcaga caaacaagaa ctcaaacatt ctggcggcgt
     1681 cgatatccga aaacagtatg agaaaatgtc cgatgaagaa ttagaagagt tggtgaaacg
     1741 gtatgaaaaa atcaacgact cttaatcgtg ttgagtatgc cgttgttcaa agagagctag
     1801 ccattcgaaa agctaggaaa agtttttggc tattctgcca gcttctttat ccagagtttt
     1861 acaaagatga tcggttgtat ttgaaagatt tatgtcatac gctacaagat ttttacgaag
     1921 ataaaatcga caaaaaaatt ttggttatta acatgccgcc tcgacatggg aagactttta
     1981 cagcgcggct attcgtttta tggatgtttg gacaaaatcc aaagacgaaa attattaccg
     2041 gatcttataa ccaaatcctg tcatctttat ttgcgcaaca aacacgtgat ggaatcatgg
     2101 ttgaaaacga aaatgttaag gctgaatact ttaaagatat ttttccagag gtgcaaatca
     2161 aatatggaga tgctgcaaag ggtttctggt ctttagatgg ttcagaagaa aagaattatt
     2221 tagcgacttc tcctggtgga acatccactg gtattggcgc gaattatgta attgttgatg
     2281 acatcattaa aaccgcagaa gaagcctata acgagcgtgt tttagattct cactgggaat
     2341 ggtataacaa cacgttggcc caacgaatgg aacggccacg taaacagata ttgattatga
     2401 cacgttggtc aagtaatgat ttagccggaa aaatgttgac gagacgaaaa aataatgttc
     2461 atcagatctg ttataaagca gtacaagaag acggctcgat gttgtgcaac gagattatga
     2521 cccacgatga gtacttagat gtggtacaag aaatgaatgt agatattgca gaagccaact
     2581 atcagcaaga accaattgac caaaaaggca ggctatatca gaagtttctt acttatgata
     2641 cgttgcctga taacattatc aaaatatgga actacaccga caccgccgac aagggtgccg
     2701 attactttgc gtctcctgtt tttgctgaga cgtctgatca tgatgcttat attatcgatg
     2761 tcatgtatac aaaagagccg atggaaacaa ctgaaatagc ccacgctaat atgattattc
     2821 gcaatcgagt taataaggta cggattgaag gaaacaatgg cgggcgtgga ttcagaagaa
     2881 attcagaaag aattacaaaa gagcgaggct atcgaggtgc agtatttgaa gactttcatc
     2941 aatcggataa taaactgtct agaatactgg cgaatagtgc ttgggtggaa aatcatgttt
     3001 actatccaga tgattgggcg acacgttggc cggactttta tgaggctatg gttagttatc
     3061 aacgtgaagg taagaacgct catgatgatg ctccggatgc agttacagga attgcagaaa
     3121 cgattataga agcagatgac ttcgagttta ttgcttatta ggaggtgggt ctttgtttta
     3181 ttctggaatc acattgaaaa aatacaaacg tttaagaacg aaatattcaa cgcaaatcga
     3241 agaaggatta ttcgatccta acgcttttat ggaagatatg aaaccctttt tcgatgatcg
     3301 agaaagaaag tatcttgctt atactagcga gaaaaacgag atcgacagtc gtgagaaacc
     3361 aaaaacaaaa atcattaagg tcaataacaa gattcatgcg ggaatgtatt cagtggtcgt
     3421 tgatcaagca gttaaccatt tcacaggaat accaattaaa tgggattatg atgtttcgga
     3481 acagaaaagg acattcattc aacgaatgag agacaaatta ctgggtaatg atatcagcgc
     3541 cccttctacc ccagaagaat ttgaaacact cacgagacaa ttaagtacta tgcggtttgg
     3601 aatgttagac cccgatactg caacatttca aggagcgaca gggcttgctt tcagattact
     3661 ggagcctgtt gagacaaatg atggttggga actaagagca agcaacattg agccgtggaa
     3721 agctgaaaag tacgaaaacg gcgcagtgta cattaaagag aaatacgatc catatcaaaa
     3781 gaagttttat gaagagatga aggttgtcac gccgaaatca atatttactt atgctcgata
     3841 tactgattac cctggcacgg tacaaggagc atttaaaacg ctgggtaatg ttcctaatcc
     3901 tttaggcgtt atcaatctta cggagtttaa aaacaataca aaccgttact gtgactttga
     3961 agttgcagag gagattagtg atgctattga tcgtgtttta tctgatcagc agaacgagat
     4021 tgaacaattc aagttagctt atatgctgat cactggggca gatttgtccg aggataaagc
     4081 caagaagatg atggagcaac tagggatcat caatctgaaa gaccctaatg cgaaagccgg
     4141 ctacgtgaca aaggatctac aaaaggattt caacgagtat cattttgatc ttcttaaaaa
     4201 gcaattctac acggtgtgta aagcaattga tttcaatgac gaagtcttta agtcgaatag
     4261 ttctggggaa gcccgaaagt ggcaaatcat tagtttagaa gctaagacaa ataccaaaga
     4321 acagtacttt cgagaaggat tgaaagagtg tgccgaggtt atggctgctt atttggattt
     4381 tgccgagaaa gttacaattg atccggaaaa gatcattttc accttctctc gttcgcttcc
     4441 aacagattta agctacctag ctgaggcatt gcctaagttg gcaccatacg tttctaagag
     4501 gacgattcaa agtcagatcc cgttcgtcac agatgttgat tatgaaaacg aaatgattga
     4561 cgtcgaaagc agacaatctt acccagacgg cgagtatgga ggtggcatcg ttgagtcaaa
     4621 tagaaacgaa gtactggacc aaacgtcgag aactggaaga tttagcaaga ctcaaaatgg
     4681 aaaatcaaac gctaaagaaa ctaagtagtg tctttcctga tgcgttgaaa gaaattcaag
     4741 cggagttatt atcccaagct gatttacacg atattaccta tgctgagatg atggaatttt
     4801 atagtacaag tgatcagaaa aaatatcgtg aatacgttga aaagaactac aaatcactga
     4861 agatgtatga tgcaaaatac aaagagttca tcgaagaata ctttccttct ttcgactacg
     4921 ccaaagtgaa tcggctgctt caaatgaggt cagatatttt tcgaattctt gcagagagtg
     4981 caatagatgc agatgtgaat aaatattttt cggatcggtt agaagatatc ttgcaacgtt
     5041 cgtatgtatc aaacgcaaac gtatttgccc agattttgaa cgtagatttg ccgaattact
     5101 tatcaaagga agaacttgaa cgttacctga actatccttg gtcaggtaag acattctcgc
     5161 gtcgtttatg gggcaatatt tcatctctag aacaaaagct atctaacgcc attacaacta
     5221 gtgtagcaag tggcgaagga gttctcgtgg cattacgaac aatgcgagaa gacccagaaa
     5281 tatgcgatat gttcaaaagg gaggaatcga agtacaacaa ggcgatcgaa aatcttgtga
     5341 gaacagaata cgctaaattc gctcaagatg gtattgagaa atcttatatt gaaactggcg
     5401 tagacgaata caatgtctta actgcaaaag atgaaaaggt atgttcaata tgcggtggca
     5461 aagcaaagaa caacccatac aagttgagtg aagcgatcat tggtgagaac cgtgcgcctt
     5521 ttcatggccg atgcagatgt acagatgttc ctaatatgcc gaaattagga aaagatattg
     5581 acgaagagta tgagcgttta tttggcgatt tattagatga gtttgcacat gattcttttg
     5641 gaattaattt gaaacggagg aagtagaatg aaagattttt ttgaagcagt actaacaatt
     5701 aatgtaaatg ctgatattgc agaagcctac aaaacagcta ttgagtctga gaaccatcct
     5761 aatggcttga gagaccattg gaatggcaat tatgcctacg tggttattgg cgatcaaact
     5821 gttaattatc aagataatac tccagttgat aagaataccg ttaatttaac gattcaatta
     5881 ttatctcatt cattaccaaa tttaaaagaa acagttgatt ggtatgaaaa tatgggatgt
     5941 attgttgtta gaactgacta caaagaagga aagtctagta attaggcttt tttattttgt
     6001 ccgaaatgac gttaaactag cgcaatactg ggcttggttg aatggtgggg cgcaactatt
     6061 aaaactcaaa gcaatgcggg gcgtgaaaac gaatcgtggg gcgaaaggag aatgattatg
     6121 aaacacaaat cattaatgcc aatgaatttg caatactttg ccgaaggtga cgatcaaaag
     6181 ttttcttttg atgacttcaa atcttttgtg gaatccaatg aagaagctca aaaatttatt
     6241 cagtcacaat ctcaaagtgt tgccgacaaa caattggaag cttggaaaca aaataactta
     6301 gataaaatta aacaggatac catcaaggaa tatgaggaat ctaagaaaaa caagtcacct
     6361 gagcaaattc aactggaaaa actacaagct gaatttgaag cagaaaaagc gttgcgtgtg
     6421 acaagtgata ataaagcatt tgttgcagaa caaattgctg gattagaact agatggagag
     6481 ttaaaagagt ctatttctca atttatgcta aataatcttg ttagttcgga tacagatttc
     6541 actaagaatg ctgttgaagg ttttacaagt gttttgaatg caattaatga gaaacatgca
     6601 gatgcattaa aagaactaca aatgaagtct gcatttggtg gaactcaaca atcgaataac
     6661 caggttcagc agaacaatga aacattaaca aatccagaag aacaattagg acaaattctt
     6721 caacaattta actaggagag tgaaaaatta tgaaaaaaac atctttaaat aatttagagt
     6781 atttggatat ttcaccagcg attaatgcta tgcaagtacc aaatacacct tttttaagct
     6841 atttacttgg tgctggaaaa acagagccag caaactcgac agaaattaaa tggcgtgaat
     6901 atgatatcaa caacgatgat tcttctgaaa aacttgaggg cggagaatat ccagatgctg
     6961 aatcaggtcg aacttggttt aacaactata ctgaaatttt tagaaaatca acctctgtat
     7021 ctggtacatt agatgctatt aatgtgaatg gagtcggaaa tgaattaact aatcaagtag
     7081 cactacgtgg tatggaaatg aaaattgatt tgaaccgaaa attgattact ggtgtaaaag
     7141 ctgatgaaaa tggttctaaa ggtcgtcgaa tgaatgggat tttgaacttg atcaattcag
     7201 caaataaggc agaaacagcc actgcgggtg cagtaacaag aaaagatatc gatactttat
     7261 ttaaattgat gtatgaaaaa ggttatatgg gagaaaaact atgcttgatt tctccagata
     7321 tgcaggagtt aatgactgat gagttagatg gaaaatcaac aaaaattgtt cagttcgggg
     7381 aaagagtaac ttttggattg caaattggaa atatcgtgtc taattacggt acaggtattg
     7441 ctctactaga accatcattg ccaaaaggaa caattgccgc aatcgatact aattatgtga
     7501 aactacgtcc attacgtgaa tggagagcag aagaacttgc aaaaacaacg gattctagac
     7561 gtatcggtct tgtaggtgaa tactctcttg aatacaatgc ttcaaactct ggggcaattt
     7621 taaatttaaa gtctgaataa aagggagtta gtactccctt ttttgatagg aggaattaca
     7681 gtggtaaaaa aagatgaaac taaaaaagat gaagtcgtga aatatagagt aggtaaaact
     7741 aaaaattttg ttgggtttgt tcatcctaaa actcgtagat ttatcacagc agattcaaat
     7801 aacgaattta tcatttctat agatgataaa gaagcaattg caattttgga agatgcaatt
     7861 gatgttaatg aaatttagga agtgatctga tggatgaatc gctaaaaacg gaaatcattg
     7921 agtctacaaa agaagatttt ccagatttga gtgaagaacg cataactaat ttattagaaa
     7981 taattttgct agaaattgaa tcatacaaca cttgtaaaaa tgatatttca tgggaaaagt
     8041 taaaaagcgt gattagtgaa gtgttgtata aaataataaa aaatgaatca gaaaaaacag
     8101 tatcttcagt tagacgtggc gatacaacga ttagttatgc ttcaacaaca aatgatgtta
     8161 gtgagttact tctaggctat ggtgatttga tacgaagagt tattggttgt ggaggattgg
     8221 agttttttta atgaatgaag cagatatttt agaaactact tatgaagata gttgtattat
     8281 tgaaagactg acggacattg aagattctaa tacaaatatt actattcaag attacaaaaa
     8341 agtatatgat aatcctattt cttgcgctct ttcacaaggt caaatcgatg gactagcagt
     8401 catagaagat ggagagatgg taaatgtttc aactgacaca tataaattat ttgttcatcc
     8461 taagattaaa ctaaaaaaag gagatcgaat aacaataact caaaaggcca gtggcttaat
     8521 tttttctcta tttgctacta agccttttta ctatcctagt cattgtgaag tgaatttgat
     8581 aggaagtgag aaaaatgggt aatctcaaat atgaatcaaa tgcagaaaag atcattgaga
     8641 attttaaaaa tatgactgta attgctcaaa aagaaggaat atccttcgtt aacgattcaa
     8701 tgaataaagt cgttagttta gctaaaccat taactcctgt aaaatcaggt aatttaaggc
     8761 gaggatatcg tgtagtaaaa gctagaaaac tatcaagcgg tcgaattgtt ggagcagtta
     8821 taaacaatga acattatttt aaatatgtag aagaaggtca taggactaaa aatggtggct
     8881 ttgtcaaagg gaaattcatg ttaactcgtg caacgaatct tgcaaatatg tcttatattc
     8941 ctcgaagatt taaacaaatg gcaataaaaa tcgttaagaa aggaaagtaa catgaaagat
     9001 gaaatcattg ctgcaatcag tagcaaatta aaagaaatct atcctgatgg gacaatatat
     9061 cttgattcag ttatgcagtc aactaaagat ttttatttcg ttttatcagt aatggaatct
     9121 ggaactgaaa atgtaggaat tgatattcaa aatgtttctt tcttaattga tattgcattg
     9181 attgataata aacctaatag aaatttaatt aatgaattag tctcacgttg tgggactttt
     9241 tttaatacga ttacaatcga tgagcaaata ttatttccaa aagcctattt acctgatgaa
     9301 gcagatggtg ttcaacatat tcgttttaca ttagaatttc cacaatatat tgaatggagt
     9361 gaaagataaa tgggagaaaa aagaagtaaa accggaatta tttctgtcga aaaacctact
     9421 tggtttcctt tggaagatga gacagggaat tttccggtat atggtactgc gttgccaatg
     9481 ggaacagcag taagtatcaa accaacagct aattatgaaa caacacaaga ttatggggat
     9541 tcagtggttc aagatcaatt tacagctttt ggtggtgcag aagttgaact ggaagcaaat
     9601 ggatatactc ataaagtttt atctacaatt actggaggga aaattgttaa aggtggtgcc
     9661 ttgcgttctg gagaagatat tgcgcaagat ggtgcgtttg catatagacg taaaaaatcg
     9721 aatgggaaat accgttatac ggtcttttat aaaggacagt ttgctctaga ttcagatgaa
     9781 acatcaacga ttgaaggaag taaagtaagt tttactcatc caacttggaa gggttcattt
     9841 gtagatgtac caggacttgg atatatgtat tcagttgatg aagatgatga aggcgtggat
     9901 aaagcaatga tcggaaattg gtttacaaaa gtagcaattc caattgaaga agcagaactt
     9961 tcaggaggta cagaataatg tcaaaatatc aaacaacgat taaattaacg aaaaaagatg
    10021 aagaaggtaa atatgagcaa gtacaattta aatctgctga atttttacca ggaacagttg
    10081 tagaagatgc tgcaggagtt atggaagaaa tgcaaacagc gactgataaa caatcagtta
    10141 aaaaagcttt aagtcgtgct tattcattta ttgcagatac tttatttgag ggacaattta
    10201 ctggtgaaga ttattgcaaa ggaattgatg ctcgtgagat tgcctcattg acaggaaaat
    10261 tattgaagtc tgttactgca ggttttgatg aaacttatac agagacgaaa aaaaagtaag
    10321 tgaggcgctc aagtcacctt catttaagta ttcgattact taccgagaat tagatataaa
    10381 gacgcaatta ctcgaagcag gttggacgtt accagaaatt gaacgtactg acttggatga
    10441 gttaatgcgt ctttttgctt ttagagatgc ggtaaaagag catgaagatg ttgagtacta
    10501 tgataatttc actcaatttt aggaggtgat actttgaata acgatgacct aattctgaag
    10561 atgatactag atgaatctgg ttttactgcc ggtatgaata atgctgtcaa aaagttgaac
    10621 tcttttgatg aaacaatcga aagaaaaagt agaaatagtg gcagttcatt aggtagtatt
    10681 tggaaaatat ttgctggaag ttttctagct agcggagtaa ctagaatcgt aggagctggc
    10741 tttgatctaa tcaagggttc cataagtgga gcggttgatc gagtagatac gatgaataac
    10801 gctctacgaa atttccaaaa tatgggattt agtaattcag aaattatgaa gaatatcgga
    10861 aagaatgggc ttttatctca aggtattcaa ggacttccta ctgctttgaa tgatgcgata
    10921 agtcatgttc aacttcttgc ttcttctaca ggtgatttaa ctcgttcaac tcaaatattt
    10981 aaagctttaa atgatggaat tcttggtttc ggtggttcaa ctgatcaagt taatgaagct
    11041 gttattcaat tatctcaaag tttctcaaac ggaaaagtag acgcacaaac ttggaactca
    11101 atgatcaacg ctcaacttgg acctactctt tctgctattg ctaaaaagat gggaattaca
    11161 atgggagatc tgaaagaagg tttgtctcaa ggtaagattt ctgttgaaga attccaaaat
    11221 caattaatag aaatggatac caaaggtggc ggaggactta aatcattaag tcaaatcgct
    11281 aaagattcta ctaagggaat taaaacctct atacaaaatg caaaaacagc tgttacacgt
    11341 ggtgttggtg aggtaataga aggattaaat aaagcattag tagattcaga tttaggtgga
    11401 tttaagggaa ttattgataa agtgggcagt tcaatggaat cgttcttaaa agtaattgct
    11461 gcaaatattc ctacagcagt atcattttta agtaatttat ttaacgaaat tcaaaaattt
    11521 ggttctgctt tgaaattcat gatgcccttt cttgttcctg cagctaccgc ttttggagcg
    11581 ttcatgtttc aacttaaagg tataccagca ataataaaaa gcttcaataa ttttaagaat
    11641 gccataattg gcgttggaaa ttcattaaag attatggggg caatagctgc tgctaattca
    11701 tttgttttaa ttattggtgc agtcgttggt gcaatagctg ttttcggtta ctttatggca
    11761 accaatgaag agtttagaaa caaggttata tctatttgga atgatgtgaa agattccgta
    11821 attggtgtat taaaaaatat aaaggactgg ggaattgata cttggaattc tgctaaagaa
    11881 atggcatcaa atgcagttga gggtgtcaaa gatgcttggt caggaacaaa ggaatggttt
    11941 tctaacacct ggaaaggtat taaggatggg gccacaggtt tatttgataa aacagtagag
    12001 acttcaagaa atgcagtcga tagtgtaaaa aatgcatggt ctaatatgaa gaaatggttt
    12061 tctgatactt ggcaaagtat aaaagattca gccagagaaa aatggaatga aattaaaggt
    12121 tctattatgt cggttgctgg tccattgatt acaggtataa agaatgcatt tttacatgtt
    12181 actttttatc tagagacctt atggaataat ttagttgaga ttggtaaaaa cgtctttgag
    12241 attttaaaaa atgttatact tgcgccggtt ttgtttatta cctccatgat ttctggtgga
    12301 tgggaagaaa caaaaaataa tatgattgct gtttggaata atattaaaga aagtgccata
    12361 aatatttggg aatctataaa gaatatattt gtaagttatt ttacaaatat ttattttgct
    12421 gcactcaata tttggacagg gttcaagctt acattgataa atatttggaa tgaagtggta
    12481 aatcaagcta aatcgatttg gttaaacata aagtattttt ttattaatct ttggattgat
    12541 ataaaatatt ttgcaattca gaaatggatt gaattgaagt tttctattat tcaaacttgg
    12601 atcgatttaa aatataatgc cattactact tggaataata ttaaacagtt tttcaaagat
    12661 acttggaaaa atattaaaga tacggcatac aatacgtgga tttctataaa aaataccatg
    12721 attaatacat ggaataacat taaggactct ttctggaata ttgttactgg aattgttaac
    12781 tccgctgaaa atgcatggac taatctaaaa aatggtgttt caaaagcaat taatcgggtg
    12841 aaagaaatct ttgattcatt aagggaaatc aatttattcg aaatcggtaa gaatatcatt
    12901 gatggactta tcaatggtgt agtagaaaaa tggaatgcat tgaaaaagac tattaaagga
    12961 atagctggaa gtatcaaaga ttctattaag ggtgcattag gtattcattc tccatctaga
    13021 tggatgaggg atatggtagg taaaaatatt gttcaaggta ttatcgttgg tattgataaa
    13081 gaaaaaagta aattggatca gacaatgact gatttagtga aaactccgtc ggttcaacca
    13141 gttattacag ggtctaatag tcaaccagtg gtacaagcta aacaaaatac atcttcaaat
    13201 gcagtgaatg aaattcactt gcatttaaat gtttatggag atctacctga ttcgatgatt
    13261 aaacaaatcg ccaagaagat gaaaacagaa ttgacgagac aaatgaaacg agatgctgat
    13321 gcagtaggag ggacattata tgcaacttaa aagaggacaa ttttttatca atcaacatta
    13381 ttcttctgag tttaacgtat atattcaaaa taggcctgcc tctgtttcag ctagtcgtgt
    13441 aattgaattg agagaacgtg aaggcaatga ctcaattatt atcgataaag cctattacaa
    13501 aaacgttact agaaaaattg aatgttatta caaagcacca tcgattgatt tagtgcagga
    13561 atgggaagat cgaattactg aatggttaga tatgaactct tacagtgatt ttattcttta
    13621 ttacgatcaa caatatatct atcaagctat agtaaccgaa gcgccagaat ttaaaggaac
    13681 aagaaaaaca gggaacatag ttccatttga atttacagtt agtcttcgac cattcaaaga
    13741 aaattatagt ggtcgttttg ctattcaaca gataaaagct ttcgagttat ttaatccaga
    13801 gaagtatgct tcaaaaccgc ttattaaatt gagtggttct ggagatgctt ctttttatat
    13861 taataatgat aaatatgatt tgaaactatt agatagagaa ttatatatag attctaaact
    13921 agaagagtct tatcgaaagc tagatgacaa tttagaacat caagatcatg tcactttatt
    13981 tttagatttt ccatttctat accctggaaa aaatgaaatc aaatggacga ataacataca
    14041 ttcgtttgag ataataccaa ggtggtggag aaaagtatga taccaagaat atatagtcct
    14101 accgagacag atttttctac gaatggttta gggattttga aggacaccac aaaatgtgag
    14161 atatatgaag tagcaaatgg aaaatatgaa ttagagttgg agtatccatt aggtactcga
    14221 tttgatgaat attttgaaaa tgactatcaa ataaaagcaa agtcaaatga tcaagaagag
    14281 tatcatatct tttttattga tgataaagat attgatacct ttttagatac cgtaactatt
    14341 tatgcccaga gtcgtacaaa tcggcttgga agacgagcgg tcacttttgc tggagtcgac
    14401 tctaaaactg gtcgagaagc aatggcaatt attgaaaaca atatggataa aaaatcagat
    14461 atacgcttat attctgatat tacaactgta tcaagtacaa cttttgaagc aagaaatgtt
    14521 ttaaattgta ttgctggtga acaaggatct ttacttcagt attggggcgg agaaattaaa
    14581 cgtgagcctt ttaagctatc tttattaaag cgaagaggtc gtgataatat tggcacaatc
    14641 cgatatggaa aagatttatc tgggttaaaa gttaagttag attggacagg cgtgaaaaca
    14701 agaattattc cgtatgctga tcctcaaagt gatgcaggta caactagccg aatttatggc
    14761 tcgccagtag atagcgaata tattaataat tatcctgatg tatatacaga acacgttcaa
    14821 ttcacagaag aacagggagt taaagacgtt aatagcttaa ataaaatagc taagaattat
    14881 ttcaaaacaa ttaatccagg ctgtgataaa ccgaagattt ctattaccgt tgagttcgat
    14941 aaattaacgg acaccgaaga aggaaaagaa ttcgctaaga taagaaatta tgggttattt
    15001 gatacgttta aaatatatca tcgaaaatat aaactatatt ttgaatcgaa agttagcggt
    15061 gttcaatatg attcattatc tgaaaaggtt ttgaaattgg aagctgggga tgctcaagtt
    15121 gctttttatc aacaacaagc tgttactatt caagataaat taaaagatta tgctacgaat
    15181 aattatatga gtagttttaa tgattatgtg tcctctatga ttgcaggtca aggaaatgca
    15241 ggcggttatg tagttttatg gccaaaagaa aaaccttcaa atatttttat aatggatagc
    15301 cctgatttaa acaaggcaaa agaagtatta agaatgaaca aaaatggaat tgccttttct
    15361 aaaaatggtt ggaatggtcc ttttaattcc gcttggacat tggatagtat attcaatgct
    15421 aattttattc aaacaggatt aataaaggca gatatctttc aaaactcttt taataaaact
    15481 ggagatgtat tgaaattagt taatggacta cttcaaattt ggaataacaa aaagaaaata
    15541 atggagttaa ccaaaaaagg aatggagttt tggaattcta atagttcaat tggaacgatt
    15601 ggaacaactg attctgctgg taatcctttt cctggagctt ctactcccac acctattgaa
    15661 gataattctt tagttattcg tacaaatgga gacggcaaat atattttgat ttctcctaaa
    15721 gttggtaaag gattagtttt attaggaaat ggtaaagcaa tttattttgg agacttagat
    15781 gtacaaggca aactcacagt taacggaaaa gaaatcacag ggaacaacag tggaggaagt
    15841 gatcctggaa ctatccctcc tcaattgacg acggaagctg aaaaaagagc atggaaaatt
    15901 tggacaatgc tgaaagctcg tggctattct gaatatgcag ctgcaggtat tctaggaaat
    15961 attcaggggg aggttggagc aagtatgaac cctgatacag aacaacttgg tggtccagct
    16021 tatgggattg ttcaatggga tggttctgct tatccgttag tcggatcacc aacttggaac
    16081 ggacgagagt atgttcagcg tttgatgaac accgcaggga ttcaagaaga ttatcgaagt
    16141 attgaagccc aagtaaaatt attagattgg tgtatgttca acggtcaatg gctcggaaaa
    16201 gtaaatccaa ccacagtatc aggatttaaa tcgatcaatg atgccaaaag tgcagcgtat
    16261 gcttttgaaa tgaacttcga acgcccagct tctgcacatc cagaacgcca aaattatgcc
    16321 caatcttggt ataacaaatt acatggatta actagtccag aacctggagg gaatttcatt
    16381 tgtccaattc aaaaaccagt gacagttact tcagaatgtg gatggagaac tagtccaata
    16441 aatggcggcc aagaatttca taatggaatt gatcttgtaa atggaaatcc taatacacct
    16501 gtttttgcag cattagatgg ggaagttgtt caggctggtg ctaattatta tgactggtat
    16561 ggtaattacg tggttattaa acataataat gggaagtgga caggttatgc tcatttgtct
    16621 cgtattgatg tttctgttgg acaaaaagtc cagaaaggtg ctcaaatagg cttgatggga
    16681 acaactggtc catctacagg agaacatcta cattttcaaa ttatgaaaaa ttattggcca
    16741 cagccagttg ttgattttga gaatccaaga aattatatcc aattttaagg tggtgattct
    16801 atgagtaaat ggaatgtcgt tttaagtaca acagaaccat ataattatgt ggggatgatt
    16861 caagttcgac aaggcaataa gaatacagag gttatggaag cgactatagt tgaaaatggt
    16921 cttccctacg atttatcaga atgtaaggta tattttgaat cagttgtagg tgggaaatat
    16981 ccagtccaat tagaaacaaa aattgtggat gctaaaaaag ggaaaattaa
//
LOCUS       DACCCS010000058        17006 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.059, whole genome shotgun sequence.
ACCESSION   DACCCS010000058 DACCCS010000000
VERSION     DACCCS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.059"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..125)
                     /locus_tag="IVK82_002113"
     CDS             complement(<1..125)
                     /locus_tag="IVK82_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP6393159.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(229..540)
                     /locus_tag="IVK82_002114"
     CDS             complement(229..540)
                     /locus_tag="IVK82_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP6393160.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(542..739)
                     /locus_tag="IVK82_002115"
     CDS             complement(542..739)
                     /locus_tag="IVK82_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP6393161.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1133..2860)
                     /locus_tag="IVK82_002116"
     CDS             complement(1133..2860)
                     /locus_tag="IVK82_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6393162.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2853..4046)
                     /locus_tag="IVK82_002117"
     CDS             complement(2853..4046)
                     /locus_tag="IVK82_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6393163.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4233..4877)
                     /locus_tag="IVK82_002118"
     CDS             complement(4233..4877)
                     /locus_tag="IVK82_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6393164.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4971..5105)
                     /locus_tag="IVK82_002119"
     CDS             complement(4971..5105)
                     /locus_tag="IVK82_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP6393165.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="IVK82_002120"
     CDS             complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="IVK82_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP6393166.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7236..7709)
                     /locus_tag="IVK82_002121"
     CDS             complement(7236..7709)
                     /locus_tag="IVK82_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP6393167.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8110..8334
                     /gene="nrdH"
                     /locus_tag="IVK82_002122"
     CDS             8110..8334
                     /gene="nrdH"
                     /locus_tag="IVK82_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP6393168.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8493..10652
                     /gene="nrdE"
                     /locus_tag="IVK82_002123"
     CDS             8493..10652
                     /gene="nrdE"
                     /locus_tag="IVK82_002123"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP6393169.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10702..11667
                     /gene="nrdF"
                     /locus_tag="IVK82_002124"
     CDS             10702..11667
                     /gene="nrdF"
                     /locus_tag="IVK82_002124"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP6393170.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11756..12895)
                     /locus_tag="IVK82_002125"
     CDS             complement(11756..12895)
                     /locus_tag="IVK82_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6393171.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13204..13917)
                     /locus_tag="IVK82_002126"
     CDS             complement(13204..13917)
                     /locus_tag="IVK82_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP6393172.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="IVK82_002127"
     CDS             complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="IVK82_002127"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP6393173.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15106..16638
                     /locus_tag="IVK82_002128"
     CDS             15106..16638
                     /locus_tag="IVK82_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6393174.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16829..>17006)
                     /locus_tag="IVK82_002129"
     CDS             complement(16829..>17006)
                     /locus_tag="IVK82_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6393175.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2910 g         5323 t
ORIGIN      
        1 gaaaaaacac attccactaa atttgatgcc ggttttcctt gaggttttgt tccagcacct
       61 cctttaattt ttttaagctc ttgatctgaa cattgtttag catttagtct atttttttct
      121 tccattacga ttccccctac aaactataaa tacttttatg tcactgattc aatataccta
      181 tttttctaat caaaaaattt tgagacatat ttaatattat aagtatcatt aaaattgaga
      241 tttatctcca taatctgctc gatataaacc atttattcca gcagttcttc caatttcact
      301 caaatttctt attaattcct cttgatattc agtgaatctt attttattag tataagcagt
      361 aaaatataga taattcacca atctatttat taaaggactg ggatctaatt catatttttg
      421 tttttctaat tttaaatata ccttttgtaa aacctcaaga atatcagaat atttaggatc
      481 tttacttata gatatatcat tatataattc atgaacaatt tgcttagcat tttttttcat
      541 tttagcactt ccctggaatt gctccaccta aaaaaccacc tatagacatt cctgcaatac
      601 aagtagttgc cttggcccaa tcgaccgtac atttattttt agtgcaatac actccatttc
      661 cataatattt tccactatga gtggtacccc catagataag ttgtgtctct ttaatagaca
      721 aaatttttaa atgtttcata atctaatcac ccctaaatta atgtttttag actattattc
      781 taacattcat taaatttcaa aaaacaattc cttcttgaaa cgtagcaaaa taattcctga
      841 ataagaattt aatcactttt aagtatataa actatgtata ttctttaata ttttttgaga
      901 atgcacttcg tacataataa actttaatca cattagtctt tcatacctac gatcgatctc
      961 tttgactcgc acccataaat tcataatagt ttgtcggcgt ctttgaacgt ttcgaagatg
     1021 agctacgcat tctctttttt tatagacaaa aaagatagag acgaacatgg agcttccaaa
     1081 tttggatatt tgatccaatt ttggaagctc catttatttc gtgtctgtct ttttattttt
     1141 tccagctttc gactaattct tggttctctt tgatccagtc ttttgctgct tgctgtggat
     1201 cttttccatt gttgatttcc aacataactt tttccatatc tttttctgac caatggaaat
     1261 tgtctaatac tttgtaagct tctggctggt cttctttcaa tccttttcga accatcgtat
     1321 ggatctgctc ttcgcttccc attccgtttt caggatcttc taagtatttc agatcatatt
     1381 tggcaaacat ccaatgaggt gtccatcctg taactacaat tggttcgtgt tttttgatcg
     1441 cttgacctag tgcaacggtc attgctcctg aagaagatgt ctctactttc cagtctttca
     1501 agttgtcata tttttggatc gtgttttcag cagccgtaac tacaccagct cctggctcga
     1561 ttcctatgat tttcttacct gcctgatcgg ttaactcatc gatcgaattt acatccatgt
     1621 aggctggcac tgctaaacca acttttgctc ctgtcaaatt ggcacctagg tcttcgactt
     1681 gatctttata ttgtgcatat tgtgaaccat gagttttagg tagccaagct gaaaccatgg
     1741 catctgactc gccatttgaa acagatttcc acatgattga attatctaat ggcgtcatcg
     1801 ttacatcata gcccatttgt ttcaatactt caccgactac atttgttgaa gcaacttctg
     1861 tatcccattc aacataagat aagttgatac gttttgtttc tttggcagat gaaaaactcc
     1921 ctataccaat cgcacctaag atcactacta ctacagcacc aatgatcagt ccttgacgtt
     1981 ttttactttt ctgtgttgtt gctcctgcag cttttttatt gtttggctgg ttcaaatgct
     2041 gtgtaaaacg gtcaatgatg attgctaaga taactaatgc aaccccgttg acaaatccat
     2101 tcccgacttg tgctctttgc aatgctgata atacgccgcg tcctaagccg ggtgcaccga
     2161 tcatagaggc aataacgacc attgaaagtg acagcattgt tgtttgattg atccctgcca
     2221 tgatcgtact tttagctaaa ggcaattcta gtttgaatag tttttgacga cttgtgctcc
     2281 cgaatgaatc agaagcttct actaattctt taggaacttg tcgaatccct aaattcgtaa
     2341 aacgaacggt tggtggcaga gcaaagatga ctgaggcaaa tacccctgga accatcccga
     2401 taccgaaaaa tgcaactgct ggaatcaaat aaacgaatcc tggcattgtc tgcataaagt
     2461 caaggatcgg tttgataata ctttgggctt tactgctttt tgccatcaag atccctaatg
     2521 gtaccccaat gatgatcgag atcacactag ataaaagaac aagtgtgaca gtattcatca
     2581 aatcattcca tagaccttgg ttataaataa accatagacc gattaatgtg aacaacgtaa
     2641 gtcctggacg tttcttagaa ataaagaaag ctgctaccgt caataacaag ataaacaaag
     2701 gagctggaat cactaacagt aaatttgtaa tccccgacat cactgcctgt ccaatcgttt
     2761 gtaaaaatga aaataggcca gaaaaagttg tcgtaaacca ttctgtgata ttttctaccc
     2821 agtttgccac tggtaattga tagttcaata gattattcat gttcactcac ctcgcttgtt
     2881 tctgctaatg cttcaatcac actacctcgg atgacgacac cttttaattt tccatcctct
     2941 acaactgcaa gtggagtcgg tgagtcataa ataatattga aaatatcact taccaccatg
     3001 tctttcccaa tcaccgtcac attttcatct aggaagtcga tcaatggacg tttttgcttc
     3061 ctagcttcta acgcttgatc tgcagtgatg atccctttca ggtgtctctt tctgtcaaca
     3121 gccatcagca tgctgacttc ttcattacgc atccttgtca atgcaacgtt tggtccatca
     3181 gattcgatat tcgtcgtcaa tgcaggtacc atgatgtttt gagccgtaag cactttggaa
     3241 cgatcgactt cttccacaaa ttcacgtaca taatcatttg ctggattggt caatatttct
     3301 tcacctgttc cgatctgcat gatttcgccg tctttcatca aggcgattct gtccccaatc
     3361 cgtaaagctt cattcaaatc atgggtaatg aaaataatcg ttttttgaac attggcttgc
     3421 aggtccagta attcgtcttg catttctcgc cggatcagcg gatcaagagc cgagaacgct
     3481 tcgtccatca gcaagatctc aggatcattt gctaaagctc tggctaggcc aacccgttgt
     3541 tgcatcccac cagataactg actaggatat tggtctttaa atgaaagcaa actagaattt
     3601 tctaatgctt tttctgcttt ttcttgtcgt tcttctttag gtacaccacg gacttctaac
     3661 ccgtattctg tattttccaa gatcgttcga tgtggaaaca gaccgaagtt ttggaaaacc
     3721 atattgattt tatgacgacg gacttctcgc aattcttctt tgcttaactt cgatacatct
     3781 tctccatcaa tatagatact gcccgaagtt ggctcaatca gacgattcaa tagacggatc
     3841 aatgtggatt tcccactccc ggaaagcccc atgatcacga agatttctcc ttcttttacg
     3901 tcaaaattca cgtcatacac accaaccgtt gcaccggttt tctttaaaat ctccgttttc
     3961 ggctggtgat cattcatcat atctaaagcg gcttgtgtct ttttaccaaa tatctttgtt
     4021 aaatgtttta cttctacttt taccaaacaa attctccttt tttatgtaaa ttcatttttc
     4081 tttctgaaaa aagtgaccac actatgttaa catcatgtag tcactttgtc aaaaatgaaa
     4141 gaaatttgct tgtccaaact gaaaaaaact tggctaaatc tttgtaaata aaaggtttag
     4201 ccaagttaat atttttttaa tatttcacac aatcattctg caagctgatg ctccgggtaa
     4261 aaaaagtttg tcatacgctg tagtgtatat tcatttccaa taaaatgaac gatgtcccct
     4321 gccgtaaatt tagcataggg gccaggtgaa acaagcagtt cttcattatg agaaattgcg
     4381 ataactgtgg cgccagtcgc ctgccagata ttcaactcgc taattgaacg gtccagatgc
     4441 attgcttttt ctgataaaga taattcaaac ggaagtaaag gattcatctt atgtattcgc
     4501 ttcgtttgat tgaccagctg atccaataat tgagaaaaat gattcagctc atcttgttgc
     4561 ttctccacac ttgccatgat ttctgctttt gtatcttgga tggattgtac atcttgatat
     4621 tgcaatagga aatcttttgc tttttccttc gaagcaacaa atgccccact tccatgccgt
     4681 acttccatga ttccaagatc taccagcaca ttgatagcct tgcgggtagt ctcaggagaa
     4741 gcattgtacg tactggcaag tgttgagcgg gcatggatct tctcacccac tttatatcgg
     4801 ttttcaacga tacgttcggc taaatcaaca gcaatctgct ggtagcgcgg gcggacgatt
     4861 tttttctttc cagacatttt ctttcctctt tcttttttta ctttccctca tcttactatg
     4921 ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg caacgactga tcatcgcttg
     4981 atctgttcct tttttactgg gcaagctgtt tgacagtcat cacaattctt accggatttt
     5041 aaacgagtat agacaattgc gcctgcactt ccaaagatta acacacttaa taagaatgtc
     5101 gccatcctat tcctctcctt ctaatgacaa tatagtataa aactggttct tggattttgg
     5161 ctgacggaac aaaacataaa cgagtcctat catcagtaaa atagcaagat acgtccctaa
     5221 cgctacctgt ccaccctcca ataagtgtcc caactgataa atcacaaaac taactaaata
     5281 agctaaaccg cattgatagc ctatggcgat ccaagtccat ttcttggcat tcatttcccg
     5341 atggatagcc ccaattgccg caaaacaagg cgcacaaagt aaattgaagg ctaacaatga
     5401 ataagctgct gcgggagtat atgtgtgttg tagcacttgc cagatttctc tgccattttc
     5461 ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca ataatatttt ctttagcgac
     5521 caatcctgta atcgctgcga ctgttccttg ccagtttccc caaccaagcg gctgaaaaac
     5581 aactgccagg attcgcccga aaaaggctaa gatactttgg tcttcagaaa caggttgagc
     5641 ataaaaatta taagaagagc tgaaccagat cacgatacta gaaacaaaga taatcgttcc
     5701 tgctttttta acaaaagact tgctgcggtc ataggtttgt cttaggaccg ctgaccattg
     5761 aggaaaatga tatgcgggca attccatgat aaaaggagct gtatctccag caaacaaacg
     5821 tgtttttttc aagctgattc cagagaaaat aattgcagtc ataccaagaa aataggcaga
     5881 aggagctacc caagattgat gaggaaaaaa ggcccctgct actaaggcaa taatcggcaa
     5941 ctttgcagaa caaggcatga aggttgtgac catgatcgtc aaacgacgat cttgctcgtt
     6001 ttcaatcgtg cgactggcca tgacgcctgg cactccacat cctgtagcaa tcagcatagg
     6061 aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt cgatccatga caaaagcgat
     6121 cctcgccata tatccgcagt cttccagaaa gctcaagcat aaaaacaata taatcaattg
     6181 cggtagaaat cctaacactg ctcccacgcc tgcgatgatg ccgttcaaga tcagctcctg
     6241 catccatccc gctacttgcc attcagctaa caatctgcca acatgttctg gtaaccatgt
     6301 accaaacaat gtgtcattga tccaatctgt ccccattgtt cctactgttt gaatagccag
     6361 ataatagatc agccacatca caaatgcgaa gatcggcaag gctaaccaac gattcgttgc
     6421 aatctgatcg attttgtcac tcatcgataa ttggaaagaa tcatttgaca caacacagag
     6481 tgtacatagt tgggtaataa attcataacg ttcattgacc agaatcgtct cactatcgtc
     6541 gtccaatagt ttctctgtta actggatcag ttgctcgatc tctttttgct gagaaactgc
     6601 tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa tatttcattg cataccagcg
     6661 tttcttgccg ctttgttctc ctagtacatc ttcaatttca tgtaaagctg cttctaaacg
     6721 tggatcatat gtacagggaa caggaataga aggccgcttt tttgcagact cctgaatctt
     6781 ttcgattagt ttttcgattc cttgcttttt caatacgctg attggtaaaa ctggtacacc
     6841 aagaccatag gaaagctttt ctagattgat tttttttcct tcttttttga tgacatccat
     6901 catattcaga ccaatgatca taggaatgtc taaatccaat aactgcgtag ttaaataaag
     6961 atttcgttct aaattggtag catcaataat gttgatgatc agttcaggct gttcctgaac
     7021 caaataatct cttgcaatca cttcttctgg tgtataagga gacaaagaat agatgccggg
     7081 taggtcctga atatgaatgt tcggattttt tttcaacgat ccttcttttt tttctactgt
     7141 tacgcccggc caattcccta catattgatt cgaaccagtc aagagattga acaagctagt
     7201 tttcccgctg tttggatttc cgactaacgc gatattcatt ctttttcctc cctttgtatc
     7261 aagattagtt cggcctctga ctttcgtaac gtcagttcat accctcgcac tgtcagttcg
     7321 attggatctc ccagaggggc tagttttcgg ataactattt ttacatttcg cgtcaatccc
     7381 atatccatta accgtcgctt cactgcgcct tgtccgtgga ttgctacgac gatcccagtc
     7441 tctcctgctt ttaattgatc aagagaagtc cagctttcag aggaagcagt ctttttattg
     7501 acaaagattt gacctagtat ttcccgactt actgccactc gatgactttt taccagtaaa
     7561 atggcattgt tgcctgataa attgacgacg acgattttac ttccggcaac gatcccgata
     7621 ttttgcaaat ggcgttttgt tttttcttct gtttcgattc gttcgacttg ataaacgaaa
     7681 ttctgctttg cctgcgctaa ggtctgcata ttgattcctc tcttgtgaaa taaatcatgt
     7741 attaaaatca ttcattatat gttctaattt tcttacgtgt tacgcaaata aatgagaatc
     7801 attctcacat agaatactct cggtaattga gaatgtcaat tatacttgtg atattatgag
     7861 cgaatcttta gtattgatag ataaaaatga gaaaaaaata tttcttcata gttttccaca
     7921 attttttctc tattaaattt gctattttcc cacacgatac ttgttttcag ctcttttttc
     7981 aaaaacacaa aacttagtat tccacagttt ccacaacaca atatgtagtg tttgttgttt
     8041 gactttttta ctaagagttt tatactatct attatagata ataagaatgt gttagaggag
     8101 gcactcacca tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg taagatggtc
     8161 aaacggtttt taacagaaaa tcaaattgct tttgaagaag tcaatatcga tgaacagcct
     8221 gaagcagtag actggttgaa agcacaagga ttccaaagtg ttcctgtcat tacttctgat
     8281 gcagaaacag ttatcggatt ccgtcctgat cagttgcgta aacttgccag ctaacatgac
     8341 taaaagaatc aaaacgtgat acatggattt gaagggcttg agacagcacc agtttttgtc
     8401 gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc aatgatttgc aatcaagcat
     8461 catcaaatac gaaatgaagg ttgggcgtac gtatgagtct taaaaattta caagatgtca
     8521 gttatttcaa gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc cctttgaata
     8581 aggataaaga agctttagtt gccttttttg aggaaaatgt caaaccaaat acgatgactt
     8641 ttccatctat tatggaaaaa atcaattact tgatcgatca agaatatata gaagcagaat
     8701 tcattcaatt gtattcacca tcatttattg aaaagctata tcaatttttg gacgagcaac
     8761 atttttcttt caaatctttt atggctgctt acaaatttta ttctcaatat gcgttgaaaa
     8821 ccaacgacgg ttccgcttat ctggaaactt atgaagatcg agtagccttc aatgcgcttt
     8881 actttgcgaa cggggatgaa gaattagcac ttgctcttgc agacgaaatg atccatcaac
     8941 gatatcagcc agctacacct tctttcttga atgctggacg taaacgtcgg ggcgaattgg
     9001 tgtcttgttt cttgactcaa gtcactgacg atatgaactc tatcgggcgc tcaatcaact
     9061 ctgctttgca attgtcccgt attggtggcg gcgtagggat tactttatca aatcttcgtg
     9121 aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga
     9181 aattattcga agatagtttc agctattcta atcaattagg tcagcgtcaa ggagctggcg
     9241 tggtttattt gaacgttttc catccagaca ttattgcttt cctttcagca aaaaaagaaa
     9301 atgcagatga aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta cctgataaat
     9361 tttatgaatt gacacgtaaa aacgaagata tgtatttatt tagtccatat agtgtcgaaa
     9421 aagaatatgg tgtcccattc tcttatgtaa atattacagc tgagtatgat catctagttg
     9481 caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa
     9541 aattacaaca agaatctggc tatccttacg taatcaatat tgatacagcg aaccgcgaga
     9601 atccaatcga tggcaagatc atcatgagta atttatgttc agagatttta caagttcaaa
     9661 cgccttctgt gattaacggc aaacaggaat atgaagtatt aggaacagat atctcatgta
     9721 acttaggctc gacaaatatc gtgaatttga tgcaaagccc taactttggt caatctgtgc
     9781 gtacgatgac tcgtgcattg acatttatta cagatgcatc tgatatcgat gtcgtaccac
     9841 cgatccaaaa cggaaataag ctgaatcata cgattggatt aggtgccatg ggcttacaca
     9901 cttatcttgc aaaagaacaa atggaatatg gctcagagga ttcattagac tttacaaata
     9961 tttacttcat gctgttgaat tattggacac ttgttgaaag caacaatatc gctcgtgaaa
    10021 gaaaacaagt tttccataat tttgaaaaat cagcttatgc tgacggcact tatttcgaaa
    10081 aatacgtaac tggagaattc cagccaaaat cagaaaaagt caaagcattg tttgaaggca
    10141 tttttatccc aactgctgaa gattggaatg ctttgaaaca agcagtaatg aaagatggac
    10201 tgtatcatca aaatcgcttg gcagtagcac caaatggctc aatttcatat atcaacgata
    10261 cgagtgcaag tattcatcct atcactcgca tgatcgaaga acgccaagag aagaaaatcg
    10321 gtaaaattta ttacccagcg ccttatctat ccaatgagac gattccgtat tacacttctg
    10381 cttatgatat ggacatgcgt aaagtaatcg atgtttatgc aacggcacaa caacacgtcg
    10441 atcaaggcat gagtttgact ttattcatgc gttctgaaat cccagaaggc atttatgaat
    10501 ggaagacaac aacgaagcaa acgacacgtg atttgaatat tttaagacat tacgctttcc
    10561 acaaaggaat caaatccatt tattacgtac gtacctttac tgatgatgca gaagaaatcg
    10621 gaagcaatca atgtgaaagc tgcgtgatct aatatcatag atagattcgc ttattcatat
    10681 tttaattaaa ggagcattct catgtcagaa acatattatg aagcaataaa ctggaatgaa
    10741 attgaagata tcattgataa atccacttgg gagaagctga cagagcaatt ttggctagat
    10801 acacgtatcc cactttcaaa tgacctagat gattggcgta cattgtctca attggaaaaa
    10861 gatacagtag gacatgtatt tggcggtttg acactgctag atactgtcca atctgaaagt
    10921 ggcatggatc agttgcgtaa agatgtccgc actccccatg aagaagccgt tttgaacaat
    10981 attcaattca tggaatccgt tcatgccaaa agttattctt ctatttttag tacactgaat
    11041 acgaaaaaag aaatcgaaga aatttttgac tggacaaata cgaatcctta tctgcaaaaa
    11101 aaagctgagc gtatcaatga aatctacaaa cacggtacac cgctagaaaa gaaaattgcc
    11161 agcgtctttt tagaaacatt cttgttctat tcaggttttt atactcctct ttattattta
    11221 ggaaacaaca aattggctaa cgtagcagag atcatcaaac taatcatccg agatgaatct
    11281 gtacatggaa cctacatcgg ttacaaattc caattaggat tcaacgagtt acctgaagca
    11341 gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt atgagcttta cgaaaacgaa
    11401 gaacgttata cagaagaatt atacgatcca atcggctgga cagaagaagt caagacattc
    11461 ctacgctata atgccaacaa agcattgatg aacctaggga tggatccact ctttccagat
    11521 actgccaatg atgtcaaccc aatcgtaatg aacgggattt caacaggcac aagcaaccat
    11581 gacttcttct cacaagtcgg aaacggctat ttgttaggtc atgtagaagc gatgaaagac
    11641 gacgattatt tgattggatt ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa
    11701 taaaaagagt ttagcgtaca gatattttaa atgtacactg aactcttttt tatgcttatt
    11761 cacttgtttc ttccagcact tttggactgt ctaacttttc cgaaacatgt tccattcttc
    11821 tcaataaata gattttccgg atatttaata ttatcgtctt aatcactgca tagacaggta
    11881 tgcaaatcag catgcctaat attcctgcga cacttcctgc tccaatcaat accaagatga
    11941 tcgtcaaagg atggatattc attgatttcc caaataacaa cggcttgatc aaatttccat
    12001 ctaattgttg gatcactaaa atcgacaacg ccatatacaa agcttgaaat ggagagataa
    12061 acaaaccgac aatgattgct ggtaccgctc caataaaagg gccgacatac gggataatat
    12121 tcgtgattcc gcaaatgacc gcaagcaaca agctataagg ctgatcgaat attctcattg
    12181 ccaaaaagct cattacacca ataatcaaag catctaagat cgtactgctg atataggcag
    12241 ataatgtttc gtttaattct tttaccgttt ggcgtaactc tttacgaatc gctttcggaa
    12301 agaagtgaga caacgcatca ataaatttgt gcccatcctt gaacataaat agcaaaataa
    12361 atggaacggt aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt tttgagacac
    12421 ttgcagataa actgactaat atgatattga acaaattact aagagataaa tttgctgatg
    12481 ctaattgttc ttccaaattg aaatttttaa actcttcccg ctgcgccaat tcatttaacc
    12541 atttgcttgt ttcatctgca tacattggca agctctgggt caattgaatt gtctgttcca
    12601 ttaactgagg cactacattc atgacagtta aaacaactaa aatgattacg accaaaaacg
    12661 tcaataaaaa accgaacact ctcgggatcc gatgcttttc gagaaaaaca acgattggat
    12721 cgaacatata atagataaaa ccagcaacaa gcaatggtaa aaaaattgcc gaaagcattt
    12781 tcaatattgg actaaaaata ttcggcattt gtaaaataaa gtacacgccg atgatcgtga
    12841 gtactagctc cactgtccag aaaaataatt ttgattgttt aaaacgtgat tgcatacatc
    12901 tcctccaagg tcctttgata cttctattct atcatttaac aaagagttac gcgctgagat
    12961 tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag acgagtactt
    13021 aaacaaaaac acggtttgga gtagtgacaa aaattatact gcagtccctt tttccaaata
    13081 aacggtgttc aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa atataaggaa
    13141 ctatttttat ttggctgggc ttattacttg atgtaggaca tctattttac aacctcagat
    13201 catttattgc gcaatactga ctaaatgcaa ctttgtgata tttttgtgta cggacaactc
    13261 ctcgatcaca tcggcataag tgagcccttt tggaagatca atcgtataga tatttttgta
    13321 cactcggtag tcatcaacga attttacgtc aaaattcaca tcttcgatta cgatattttt
    13381 ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt ctatggataa attggatttc
    13441 taacttttta gtcgtaggta catggatcac gcgtttcact actgtgagtg caatagcaat
    13501 cccgataaaa ctgatgatcg caatggaata gtaacccatg ccgatggcga tccctaatcc
    13561 agcaattgcc cataaagaag cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat
    13621 gatcgttccc gcgcctaaga aaccgacacc actcaccact tgagcaatca gccttgcttc
    13681 atctgaacga atcgttccag caagctgtgg atactctaaa gcatctctta atgcaccact
    13741 agcgatctca acttggatca atgcaatgat acatgctccc atacaaacta aaatatgcgt
    13801 acgcatacct gctggacgat ttttatattg tcgttcaaaa ccgattgctc cgccaaatag
    13861 catagcgatc cctaaacgta aaataatctc tggaattgtc aataccatat cattcatcta
    13921 aacaccctcc agtccaatat atcatgtctg aaaggagcag acaaaaaaag tgcaattttt
    13981 acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt atgaataaac
    14041 ggtgttcaat cagctgaccc tcggtattac tcaacaaacg gcaaaatatg gggagctatt
    14101 tttcctttgt cttctctaat actcaggtca aaacgctgat ttcacaacct ctttagccta
    14161 ctattgaaaa ttacaactta cttgctgctt cttcatcgat gatcacaaca acattgtcgt
    14221 gattttgcaa tacacttgct ggcaaatgat tcgttacagg tccttcaatc atccctttga
    14281 ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat ttctttactt ttcataattg
    14341 aaccgattcc cattgaaaca gcttttgttg gtacatcttc tgctttatcg aagaatcgtt
    14401 tgtttgcatt gattgttgat tctgttaagt caactactga agtcgttccg tcaaatgaag
    14461 caccaggttc attgaaaccg atatgtccat tttgcccgat tcctaagatt tggatatcga
    14521 ttggatgtgc atcgatgatt tgatcatact ttttgcatgc tgcttccaga tcttctgctt
    14581 ttccatctgg tacaaacgtt tctttgaatg gttttttatc aaataattgg acatccatga
    14641 aatgacggta gctttgttca tctgaaccgc ctagtccaac gtactcatct aagtttacag
    14701 aagtcatttc actgaaatcc aaatcgcttg aagtcatttc tttatataaa gtaactggtg
    14761 tgcttcctgt tgccaatcct aatacttttg cacctttgtt cataccttct ttgatgattt
    14821 caaatgcttt ttttccgcct tcttcagcgt tttttacacg aatgatttcc atagtgattg
    14881 ctcctttata ttttggtata gcccaattat agcattcttt ttttcttttt acaataatta
    14941 gtatagacca aattgcattt ttttcatttt cttgacaata aataggaatc cgtgttaggt
    15001 ttcataagat ggttcaatca aacaatccat gttgcatgaa aaccaagcaa gtgattcact
    15061 gtggacgctt tatgtctgca gtttttttat gaaaaaggag ttattatgag tatactgaca
    15121 ttaaacaaca ttacccagca atttggcgac aaaatcctct atgaaggtgt cgatctgcaa
    15181 ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg gagccggtaa aagtacattg
    15241 attaaaatca tcactggtga gatcttacca gatgacggac agatccattg gcaaaaaaat
    15301 attcacaaag gttacctcga tcaatatgtg gaagtcgatg aaacattaac aatcgaagaa
    15361 tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag aaaaaattgc ttttctttac
    15421 caaacatata gcgagacctt cgatgattcc ttgctggaaa aagctgggaa actccaaacg
    15481 gaattagacc aaggagtgtt ttatcaaatc gacaccttag ttgctgagat gagcagcggg
    15541 ttggggatcg atgtcttagg tttagattca ctgttgaaaa atctaagtgg tggacaacgt
    15601 tcaaaggtga ttttagcaaa actactttta gaaaacccag atgttctgat cttagatgaa
    15661 ccaaccaatc acttagatga tcagcatatc caatggctga cgcagtttct tcaggatttt
    15721 gacggtgcgt atatcgtgat ttcccatgac caagaatttc ttgatcagat caccacccat
    15781 atcgcagaca tcgagtttgg aaagattact aagtatactg gccatctaaa acaagcattg
    15841 aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat atcatgctca acaaaaacaa
    15901 atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg gttcccgatc aacgatggca
    15961 aaaagccgac aaaaacaatt agataaaatc gaacggctga cgcctccagc ttcagcaagt
    16021 aaagctgtct ttgattttcc attctcgcct attgtcacga cattagcagt ggaaacaact
    16081 gatttagtca tcggatatga tcgaccgcta cttgcaccca tcaatttgac catccgcttt
    16141 ggtgaaaata ttgccattcg aggcttcaac gggattggaa aatctacttt attaaaaaca
    16201 ctgatcggtg agattgaaaa aataagtggc gatttccact tcccagataa cacaaaaatc
    16261 aattactttt cacaagatct gtactgggaa aatccattag aaacaccact acaatattta
    16321 agcagtcaat ttccaaaagc gactatcaaa gaattacgtc gccaactagc taaagcaggg
    16381 ttagttaatc aattagcaag tgaacctctc actacattaa gtggtgggga acaaactaag
    16441 gtgaaacttg cacagttgac aatgaaccaa ggtaatttac tgattctcga tgaaccgacg
    16501 aaccatatcg atcaagaaac gaaagaaagc ttacaagacg ggatacagaa atttccagga
    16561 acagtaatca tcgtctctca tgaacaagaa ttttatcaag atcttgtgca acgagtcatt
    16621 gagattgaac caaaataaac gtttgattga atagttgaaa aaaaggagct gtgacaaaag
    16681 ttttgtcaca actccttctt ctgaataaat ggtggccaat tagctgacct tcggtattag
    16741 ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg ctattcttat tacttgatgc
    16801 agaacagctt ttcacaacct cttttcgtct attttttatt ttcgagattt ctctttcctt
    16861 taaaaacaac tgtttgaaag aagaacgcac atccttgaaa aacaagcccg ctaaccgtta
    16921 cacctaacca gccatactgc tgccacattg tcgtccctag caatgaacca gccgcacctc
    16981 caataaagta cataaacata aataca
//
LOCUS       DACCCS010000059        16892 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.060, whole genome shotgun sequence.
ACCESSION   DACCCS010000059 DACCCS010000000
VERSION     DACCCS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.060"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(44..410)
                     /gene="ssrA"
                     /locus_tag="IVK82_002130"
     tmRNA           complement(44..410)
                     /gene="ssrA"
                     /locus_tag="IVK82_002130"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(581..1354)
                     /locus_tag="IVK82_002131"
     CDS             complement(581..1354)
                     /locus_tag="IVK82_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6393176.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1498..1848
                     /locus_tag="IVK82_002132"
     CDS             1498..1848
                     /locus_tag="IVK82_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP6393177.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1829..2545
                     /locus_tag="IVK82_002133"
     CDS             1829..2545
                     /locus_tag="IVK82_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6393178.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2545..3993
                     /locus_tag="IVK82_002134"
     CDS             2545..3993
                     /locus_tag="IVK82_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6393179.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4063..4572)
                     /locus_tag="IVK82_002135"
     CDS             complement(4063..4572)
                     /locus_tag="IVK82_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393180.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4562..5287)
                     /locus_tag="IVK82_002136"
     CDS             complement(4562..5287)
                     /locus_tag="IVK82_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393181.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5545..6885)
                     /locus_tag="IVK82_002137"
     CDS             complement(5545..6885)
                     /locus_tag="IVK82_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6393182.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(7052..9172)
                     /locus_tag="IVK82_002138"
     CDS             complement(7052..9172)
                     /locus_tag="IVK82_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP6393183.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            9402..10580
                     /locus_tag="IVK82_002139"
     CDS             9402..10580
                     /locus_tag="IVK82_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP6393184.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            10596..11354
                     /gene="aroD"
                     /locus_tag="IVK82_002140"
     CDS             10596..11354
                     /gene="aroD"
                     /locus_tag="IVK82_002140"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP6393185.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            11377..11862
                     /gene="ybaK"
                     /locus_tag="IVK82_002141"
     CDS             11377..11862
                     /gene="ybaK"
                     /locus_tag="IVK82_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP6393186.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11933..13186)
                     /locus_tag="IVK82_002142"
     CDS             complement(11933..13186)
                     /locus_tag="IVK82_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6393187.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            13303..14652
                     /gene="gdhA"
                     /locus_tag="IVK82_002143"
     CDS             13303..14652
                     /gene="gdhA"
                     /locus_tag="IVK82_002143"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP6393188.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            14764..16110
                     /locus_tag="IVK82_002144"
     CDS             14764..16110
                     /locus_tag="IVK82_002144"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP6393189.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(16418..16804)
                     /locus_tag="IVK82_002145"
     CDS             complement(16418..16804)
                     /locus_tag="IVK82_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6393190.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5432 a         3047 c         3451 g         4962 t
ORIGIN      
        1 aaaataagga tttaagcatc tagcgaaatc tgctgaaatc tcacaatgga gacggcggga
       61 gtcgaacccg cgtccaaaca catcgccact taaaaatcta cgttcatagt caactcattt
      121 aaggattcac tttacagctt gccgagtgac aggcattctg tatcgctagt ctgatgttct
      181 ctttttcaac ttacagacgg aaagttgaaa cgtatcccac taaagttagg acccttactc
      241 gagcacatgg gcgatgccga gaggatctac gctaactgtt tttaggcagc taaagcgtaa
      301 gagttgtttt cgtttttagc agttatattt aactgtaacg tttttacgta gacgtaacct
      361 acgaaacgca attcaagctc gactgtgcct gtcgaatccg taacgtcccc gttagctaac
      421 aagggttacg tgtatttttc atacaaaatt agtatagcat atattgggat aaattcaata
      481 aaaaagcgaa tcatatccca ttatttatat aataactgcc cctttcttaa gaaaaaataa
      541 gcgattttct taagaaaaga ggtagcttca aaaaaagatc ttaaatcaga tcaaaatact
      601 tcaatgcgtg gacaattccg ccttctgtat ttttctttgt gataaaggta gaaagttctt
      661 ttaatccgtc ataggcgttg cccatagcga ttttgttgtc gcaagcttcc aacaaggcaa
      721 aatcattcgg accatcgcca aatccaaaag taggaacttt ttccagctga agttcttttt
      781 gcagacgttt gacacctgcc ccttttgaga ttcctttctt cactgtatcg atcgagtaag
      841 gaccattccg ataaaaagtt agctcaggaa aatgttcgtg atagtattcg tcacctgatt
      901 gagataagat cagcaacata tttactgtgt tttctttaaa gcctaaagga tcaatttccg
      961 gaacatctga atgaatgaag tcgtaagcat tgatcattgt ttggctatga ccggtacacc
     1021 agattttctg atcgttatag taagaaagtt catgaccgtt caactgtaca tgatcataca
     1081 ttttttgaca ttcttctgaa gaaaatgttt gagaatagat ttcttcgccg tctactcgaa
     1141 tgaaagaacc attcatggtg accgcagaag taatgcccgc atcttccatg atagagtgga
     1201 tttctgcttc tgtacgtcct gtagcgatga cggggagaac attgttttct tttaatgcgt
     1261 tcatagcttt tgccacttcg ggtgtgattt ttgatttcgc atctagtaat gttccatcaa
     1321 gatcaaagaa agtgattgca cgataatttg acacaaataa aactcctttt ttattggaat
     1381 aagtttatct taacaaagaa tgaacaaaag acaaaccttt cgtgaagaat tcctgaacat
     1441 attggttcag tcaacaattt agagtatact ataagaagag aatttaggag ggaaagaatg
     1501 atttttttca gtattggtat cttattctta ataattggca gtctttttcg gattctgccc
     1561 tcaaaaggaa atctgccttt ttatgggtat cattcgcctc ttgccgcaaa aacagacgct
     1621 cattggcggc tggctcaaaa gacaagcgga aactggtttt ttcttatggg cttactgatg
     1681 gcattgatcg gctattattt aaagacatct gggcatacga attattttct gattgaaatg
     1741 cttctgctag tatttccaat tatgcctatt tttattatga cagaaaagaa attacagaaa
     1801 tatgacctgg aaactggagg gaacgataat gaatatttta atgattgaag ataatcaatc
     1861 tgtttctgaa atgatgcaga tgtttttctt aaatgaaggt tgggaagcaa ctttcaagta
     1921 tgatggaaaa gaagggctcg atgccttttt agaaaaccca caacattggg atatgattac
     1981 actagacctg aatttgccca cactggacgg tatgctagta ggaagagaga tccgcaaagt
     2041 ttcaagtact gtgccgatta tcatgctgac agctagagat tctgaaagcg atcaggtgat
     2101 cggcttagag atgggggcag atgattatgt cacaaaacct ttcagtcctc tgacgcttat
     2161 tgctcgaatg aaagctttgc atcgccgtag tgagttggca gaagcaagag aagaagcagt
     2221 agtttcagat gatcattttg atatcgagac tagtcatttc aagatgaata cgaaaacacg
     2281 agaagcgtat ctggacaacc ggccaattga aggactgacg cctaaagagt tcgatctatt
     2341 gtttacttta gcaaaaaaac cacgtcaagt attctctcgg gagcagttgc ttgaattagt
     2401 ctgggattat cagtactttg gtgatgaacg gacagtggac gcccatatca agaaattacg
     2461 acaaaaaatc gaaaaggtcg gaccacaagt catccaaact gtttggggag tcggctacaa
     2521 attcgatgat tctggtgttg cctaatgcgt tatttgtatc agcaactctt agctttctgc
     2581 ggaatggtcg cgttgatctt attgatcgtt ggagtttctt ttacacaact gacaaaacaa
     2641 acgattgaag aaaacaatta ccagcaactg tttggatatg ctgaatcagt tgaaaagaca
     2701 acgcaaacct atgccgatac gctcccgcag tttagtcaag atcaggcgtt tcaaaatgca
     2761 ttgctgctga cagagcaagt actgacagaa caaaatgtga actttatatt tatcgataaa
     2821 aacgaacgtg tcatttatcc gactaataca gctttactga attttacctt aacagctgat
     2881 caatggaaaa atctgaggaa tggacgtcaa gaaaaattca catcgaataa aaatatttta
     2941 ggacaagcac aggcgacctc ttacgcactt gtgccattta atttgaatca tgaattctat
     3001 ggagcacttg tagttagtca acctgcgcga aatatcgata acagcgttcg tccaattaca
     3061 ctgaacctgt tcaaaggatt tattttttcc agtatcgttg cagttattgc cagctatgct
     3121 tttgcagcat tccaagttaa gcgtatcaat cggttaagga atgcgacgaa agaagtgacg
     3181 aatggtaatt ttgatgtaca gctccccgta catgacaaag atgaatttga tgagttagca
     3241 gatgatttca ataaaatgac gaattcattg aaagaatctc aagcagaaat tgaagaacaa
     3301 gaaaatcgtc gaagacaatt tatggcagat gcttctcacg agatgagaac acctcttacg
     3361 acaatcaacg gacttctgga agggttagaa tacaatgcaa tccctgaaaa tcagcgagaa
     3421 aacgcgatta aattgatgaa aaatgaaaca gagcgtctga tccgtttggt gaatgaaaac
     3481 ttggattatg aaaaaatccg tacgaatcaa atcagtatgg tcattaaaaa attcaatgga
     3541 acagaaacat tgaaaaatat tgttgcacag ttagaagcta aagcagaagc agcaggagat
     3601 acattgacat tgaaagccgt tgatgatatt gatgtttacg ctgattatga ccgatttgtc
     3661 caaatcatgg tcaatattat tcaaaacgcg atacagttta ctgaaaacgg ccaaatcacg
     3721 attactttag aaaaaggcta tcttgaaacg atcatcacga ttgaagatac gggaattgga
     3781 atgtctgaac aacaaatgaa aagtatctgg gatcgttatt ataaagtaga tccttcaaga
     3841 aaaaatacaa aatatggtga atcaggatta ggtttaccaa tcgtgcagca acttgtacgc
     3901 ttgcataaag gaaaattaga agtagaaagc gaactaggaa aaggaacaaa attcgctgtt
     3961 cgtttgcctg acgtagaaat caaaaaagag taaaaaacaa gcggtataaa agatcatgaa
     4021 tcgatcttct ataccgcttg tttttactat aaaatgattg atttagcccg cgggctctaa
     4081 acgatcaata taaggacttt gagcaatagt agcatcatga gtaaagagta agataccttg
     4141 attttcttca tgagctaaat aagaaagcaa gtggagtagc tcttggatct ccaatacact
     4201 aagttcatca aatgtatctt caataacaat attgtttcgc ttggaaagca aggcatgaag
     4261 taattgcagc tttacttttt caaaagacgt caactcagct gccgcacaat taagcaagga
     4321 aggagagata tcaagttttt cttgataggc caaacgatct tgtttgattt ctttttccgg
     4381 tacatccaac aggacatggt ctttaagatt cagataagga aacatcggcc aatttttttg
     4441 gatgatcgca aaattatcca gttttgcaac aagaacctct tttttcaagt gctccaatat
     4501 tttatgctgt ttttcgagag gacacaaaat aacggtcaag tgttcagcat gaatcaaata
     4561 ttcaggtttc attacgtcca ggcctcctac tttgcaaatg atggataaaa aaagtgaaag
     4621 ggaatagact aagaatgaga cagcatccac tgagcccaat aagagtttgg gcaaatccct
     4681 taagtatatc tgtgaaaaaa agatcatttt ggaaatcgat ggtaaaaaaa gtctgttgat
     4741 ccatcggaat tgtaagtccg cttaccgaag tcaagatatc acggatatct ggcagttcag
     4801 tagtctttaa gacaagaaaa ttgatccttt gaagaagcgc ttcataaact gactggaagc
     4861 ttacgatcca caataaaacc aagatacaac aaagaaagag aggaaagaac agctgccaga
     4921 aaataaaaag gaataatttg gttttagaaa gaccagtcag gtgccaattg atcaattcct
     4981 gcttgttttg cctgactgaa tagtaaaaga aaaagataaa aaggaaggca aatagaaaaa
     5041 ataaactgaa tatgcctttt atataaaatc ggctagctaa ttctaaagcc ggaagaagct
     5101 ctgtagatgc agaagcgcca ataaatgaaa gccgatcttg gacttgattg gctaaagccg
     5161 attgcaaatg gtaaagagtc aggcatgcta tcaataaaaa agcagaaaag cttgaaaata
     5221 aacaatagag tattgtctgc tttttgtaat aaattaatcg agcagtagca tagcggatta
     5281 ttttcatact tttcacctct ataaaaagta tagaaaatga atatgaataa agtatgaaat
     5341 tcctttagtg aaatcacgaa ttaaaaccag aaaatatttt gtaaaaagca agaggttgtg
     5401 aagaagctgc cctattctat taccgtagat gcaagctttt gaacactgtt tattcgaaaa
     5461 gaggagcagc gacaataatt ttgggctctt cgtcaatgag tgttggtgaa gaaattcaat
     5521 agaataatcg agatactcac acggctagac agctatcttg tctagccgtg ttgttgttct
     5581 ggctttagtc agttgggtag tgttcttagt tttcttgaaa tattttcctc gatataaaaa
     5641 gattcgttct cggtagtttt gaaagtttct aaagccatag gcaattcgtt ttagtacttt
     5701 gatatggttg ttcaaacatt ctatagggcc gttggaatag ggaactcgca acgctcgttt
     5761 gatttctgtt cgatatttct tgaaggtcgt gaacactggt tgatagtgct ctggcaactc
     5821 cttgtagtct tcttttagga gcgcgacgaa ttcaggaaca tcttgtcttc tgatagcgga
     5881 aagaaaaacc tggtagactt cgtagcctcg ctttaaggag tcatcataat ataatagccg
     5941 atcgactatc tccgcttctg tcagatgatc tcgaaatgac ggacgccagt agcgatgctg
     6001 accattcaat tcccacgcat tcttttgaag tagtctccag tatttcttta agtgcttcga
     6061 ccgacgatcc ccttttttaa aggtcttcat tacttgaatt cggtggttgg taaaggcccg
     6121 accgatgtgt tggctaatat gaaagcgatc ggcgacgatt cgggcattcg gaaaacactc
     6181 tttcaccaat gtgcgatagg gtgtatagaa atctgaaacc actagacgta cttgttcacg
     6241 gacttttcta gggtagcgca agaaatagtc gcgtaaagga ttgagttgcc gattttccac
     6301 aatatcaatc agttgatggg tcttgccgtc catcatgaca aagctcatgg ctcccgagac
     6361 gtttttcacc gatttgaatt catcgaaaca caccacttct ggtaacggct ggttcaaaga
     6421 aacggtttta ggccgaaggc tgcgaagtat tcgaatgaca gtggtcggag aaatgtgctt
     6481 catacgtgca atcaaagaca tagaaatagg ctcacttaat agctcaagaa tggcttgttt
     6541 gactctgcgg gcaatggagc agcgacgatc gctaactgaa tcttctgcta aaaaagtctt
     6601 tccacagtgt ttacatttga agcgctgttt gttaatccgc acaatggtct tgtagttact
     6661 gacatcattc aataagatgg atactttttt ccagccccat ttaatgatcg tttgcttatt
     6721 ttgatagtgg caatggaagc attcctctgg ttgataggtc aatgtgccat caaaaacaaa
     6781 acatgtttcg tcattaatgc gctctttgcg aaaggcatct tcatgaaagg taagatttag
     6841 gtctagaata tctagtaact ctttggtaaa ctctgaatag gacatcttga aacactcctt
     6901 tgaatggttt tcgtcgactt tattctaaag gatttcaagg tgtctgtttg cgtttattag
     6961 aaaaaagaaa aagtattggc aacagaaggc gtcaccaaca ctagatatta tagatcctaa
     7021 ttttgtcgct gctcctttta aatttatttt atcattcgga agaacttgtg gtcgttccgt
     7081 gaagttcttg gtacgtttgc gttttataaa gatcaacagt tgattgtccg ttgtgttcac
     7141 taaagacact agttgacttg tcaccaagag atttttcagc ttcttttaat ttctctaagc
     7201 cattcttgta gtcataatca ctggtatcaa ttgggtctaa tccactattc gtatagaacc
     7261 gaagaagatc accattattg atttggtcag aaacagctaa ctgtttatca actttttctt
     7321 tcagtgaatc aatttcattc tgtacttcct cagtaggttc agtaatagct tcaccagttt
     7381 cgttactata gatcgtacca ccgtagtaag tatattttgg tgtaacaaaa tcaccatcac
     7441 ggaaagaaac aagctgttca tgatctttcg agaatagatc ttgtcctaac tgaatataat
     7501 tttttgtatc tacacctagt aagtgaagta atgtaggcaa ggcatcaact tgtccgccgt
     7561 aagtgtgatt gattccgccc tgtgtttgtc cagggatatg gatcatataa gggacacgct
     7621 gcatttgtgc attgtcgtag tttgtccaag tagaacttgt ttttccaact aattcagcta
     7681 gatttttatt tcttgaattc gagacaccgt aatggtcacc gtaaaggacg atgactgaat
     7741 tatcatacaa gccgctttct ttcaaataat tgaagaattc ttgtacggcg gtatctaaat
     7801 aattggctgt cgcaaagtaa ccgttgatcg tttcatcatt tgtattggct aacgggaatc
     7861 ctgcttcgtc attggtgaat tttgaataag gataatggtt agatacagca atgaattttg
     7921 aataaaatgg ttgttgcaga cgttccaagt attgtacgga ttgttggaaa aatggtttat
     7981 catgcaaccc gtattggaac gaattgtcat cattaacatc ataataagaa gaatcaaaga
     8041 aataatcata tcctaaattc ttgtatgtct cgtttcgatt ccagaatgta ccagcgttcc
     8101 catggaatac cgcactaatg taaccttgtg tttgacttaa aatatcaggt gcagcttgga
     8161 aagtattttt tccgcctaat tgtgtgaaca aggagccttg attcaagcca aacaatgaat
     8221 tttccatcag cgtttccgca tcgcttgttt ttcctgcttt tacttgatgg aaaaagttgt
     8281 caaaactgaa cgtgctgttg ctgtggtaaa gactattgat gaaaggtgta acttcatgct
     8341 ggagaccatt ctcatctgtc aatttataat cgatcaggaa ttgctggaag ctttctaaat
     8401 ggatataaat cacatttttt cctctagcga gcccgaacaa atcgtcattt ggtgctgcat
     8461 aatgttcttt tacataattt tctacttctt tcatatcatt gggacttgct tctgcccgca
     8521 cttggtttgt ctggtaagtt tgaacaccgt cataaaccat gaaagcattc aagccaagat
     8581 atttgaccaa ataatcacga gagaatgttc gagtcagcaa ttctggacga tcagcttccg
     8641 ccataaaaag atttcctgag aaaatcataa ctgctaatgt agagatcgca aaagccattc
     8701 ttgctcggat cgggcgaggg tccaacttga ttttttttgc aatcagcaga acagtcaaga
     8761 taataaaatc gatccaatag atcacatcat atgggcgaaa cagtcgaatg gcactttctc
     8821 ccagaccact cgctactttc ccagcaccta aaatcgtgtt gatcgtaatg aagtccgtga
     8881 attctcgata ataagtgaca ttcgaaaaaa caagcaatgt caaaagaaaa taaatgacca
     8941 tcatcgtgat ataagcaact ctagggcgtc tgatataaag agcaattcca agtaataaca
     9001 atgtagttgc aatagggttg ataaacaaga taaagtattg aatgccactt tcgattccga
     9061 gatggaaatc aactaaatag gcaaaaatcg ttttcaacca aagtaaaacg actagtaaag
     9121 aaaaaaatcc gagcctctta ttcaaaaagg caggtgtttt tatacatttc aagataatct
     9181 tcctttctgc acttcttaat cacagaagtg tcctgaaacc atgttacaca gctcacttat
     9241 tctcatttat tttactagta tgacaatata gtgtcaattg aaaccagaag tttatgctgt
     9301 ttcattaagg ttttctttaa gcttcatgaa aaaatgagca gtaagacaag tcctatgttt
     9361 tttgttatac ttgggaagag aaagctaaag caggtgaagg aatgaaagta aaagtaaaaa
     9421 aaagagcaag caatcgattg aaaaaaggat atccactgat ccaaaaagaa gaccttgctc
     9481 atccccaaaa agaaaccacg caatgggtgg aattagttga ttcgcaaaac caatttatcg
     9541 caaaaggata tctaggaaag cagaataagg gaatcggttg ggtattgagc cagcagaaca
     9601 tcccatttga tcaatctttt tttgaagaat tttttgtgaa gtcaaaagaa aaaagaactt
     9661 tctttttttc agatgattct actaccgctt ttcgtatatt caatggagaa ggagacggga
     9721 tcggcggtct gattgttgat tattatgcag gttatgctgt tttttcttgg tataacgaaa
     9781 cgttatatgc acatcgtgaa ttattgattc aagcttttcg tgaaatctac ccggaaatca
     9841 taggaggcta tgagaaaatt cgatttgaat cagtaaatct gcctgagagc cagttcattt
     9901 acggaaaaga ggcaccggaa cccttgatcg taaaagaaaa cggagtaaaa tttgccgttt
     9961 atttaaatga aggattaatg actgggatct ttttagacca aaaagaagta cggggatcat
    10021 tagcagaagg tttcgcttct gggaaaacag ttttgaacat gtttagttat accggcgcct
    10081 tttctgttgc tgccgcaatg ggaggggcaa tcgaaacaac aagtgtcgat ctagcaaaac
    10141 gcagtttaaa aaaaacacag gaacagtttg aagtgaatgg tttggatgtc gatcagcaaa
    10201 aaatcattgt tatggacgta ttcgagtatt tcaaatacgc caaaagaaaa caatttttat
    10261 atgacatgat tgtattggat ccaccaagtt ttgccagaaa taagaaaaaa gtctttcgcg
    10321 tagcaaaaga ttatggggaa ctggtcaagg atagtctgga tattttagca gaagacggga
    10381 tacttattgc gtctacaaat gctgcgaatg tctcgataga acaattccaa acaatgattg
    10441 aagaggaatt ccatgccaaa aatgtttctt ttgaacaact tgctttatat cgtctgccaa
    10501 aagatttcca gacgacggat acctttttag aaggcaatta tctgaaagtg tttgtttatc
    10561 aaattaaaaa taaccattag aaggagcgat gcagaatggt tcgggtaaag ggattgaata
    10621 taggaagcgg tagaccaaag atttgtgcgc ctcttatcgg ggccgtccga gaggaagtat
    10681 tacgtgaagc agttcttgca cgtgaagcag gggccgattt agtcgaatgg cgagtcgatc
    10741 attatcgtga agttttttct tttgatgaag tagcagaaac attggttctt atccatgaga
    10801 tgcttgatgg actgccgtta ttatttacgt ttcgtacctt agctgaaggc ggagaacaag
    10861 acatatcgat ccgtcagtat cgcgaccttt atgaacatgt gatacacaca ggattagtag
    10921 atatggtcga tatcgagttg ttcaaagtag aaagtctagg aaaaagactg atagaggaaa
    10981 tcaagtcttt aaacattcct ttaattatca gtagtcatga ttttaaagaa acaccagcag
    11041 accctgtctt gctttaccga ttgaatgtta tggaacattt tggagccgat atcggaaaac
    11101 tggcagtaac tcccaataat gaacgagatg ttttgcggtt gatggagttg actagacggg
    11161 caaatgcttt tttctccatg cctattatta cgatgtccat gggaaatctt ggtaaaattt
    11221 ctcgtctggt tggcggaacg acgggttccg ttatgacatt tggttcctta agcagcgatt
    11281 ctacttctgc tccaggacaa ttaccggtca aagcattgaa gcaaattatt caattattaa
    11341 atgaaccgtt gtagaacaag tttgaggtga tattttttgg caaagaaaaa aatagtaaaa
    11401 acgaatgcaa tccgtatggt cgaacaaaaa aagattccct atacagagca cgaatatgag
    11461 tgggacgaaa gccacctaag tgcgtcatcg gtcgctgaac agcttccaga aagccaatct
    11521 cgtatattta aaacactggt tgctgtgggc aatgtgacgg ggcctcttgt tgctgtgata
    11581 ccgggagaag ccgaattgaa tctaaaaaaa ttagcaaaag tttcagggaa taaaaaagta
    11641 gaaatgctcc atttaaaaga tttggaagca acgaccggat acatacgtgg tggctgctcg
    11701 cctataggta tgaaaaaact atttcctact tatttagatc aaattgcaga aagctatgaa
    11761 caaatcattg tctcggctgg cagaagaggg ctacagatgg agttagcacc tcaagatatc
    11821 tgtgcattaa cgagcggaca atttgcagat ataaaacaat aaatcatacg tataaaagaa
    11881 agaaggaggc cgggatgttg tttgtccagt ctccttcttt cttttttata cattatgctc
    11941 tttgcccggc attcattcct gctacatgcc ctgtagcgaa agccgctgtg atattaaagc
    12001 cgccagtata accattgaca tccagtaatt ctcccgcaaa atacagacca tcaatgattt
    12061 tactctccaa tgttttagga taaatttcct ttagagaaac ccctccgcca gttacaaatg
    12121 atttctcaat aggaaaagtt ttgttgattg atagcttaaa ctctttgcat agttgaacaa
    12181 aatcttgaat ttgtttttga gaggcttgtt gtcctgtcag atgatccatt tcaagccgct
    12241 caagaaagaa aactaacaat cgctcaggca atataccatg ccaagcattg accaagttct
    12301 ttttggttgc tttacttttt tctgtcaata tatgaataag ctcttgtttg gtttgtgtag
    12361 ggaaacaatc cagagaaagt gtcactggtt ctcccgtttt ctctaattct ttattgacaa
    12421 aacttgaaca acgcagtgca gccggaccag aaataccgaa atgggtgaaa agtaaatcca
    12481 tcgtatgttc agtcaataca cgaccttttt tatttaaaac gcgtaaagtg atgtcttgta
    12541 aagaaagccc ttgaagtgtt ttctcctgaa tatacggttc atccgatatc aagggggact
    12601 ctgtcgcata aagaggcgtc accgtgtgac ccactcgctt cacgatcttg tacccatctc
    12661 cagtagaacc agtagatgga taagtccgtc caccagttgt taagataatg gcaggagcat
    12721 ggaactcttc gaattccgtg cgtataccgt aaattttatg ttctttgtgg atcaatttct
    12781 ctactcgagt agagaataga agcgtcacat ctagttcttt cagacgattg agcaatgctt
    12841 cgataatcgt ttttgattta tttgttacag gaaacatccg tccatgatcc tcttctttta
    12901 aatgtacgcc ttgtgattca aagaagttca tgatatcgaa attattccat tgcgcaaacg
    12961 tactatataa aaactttcca tttccaggaa tatgtgcaat caaatcatca acaggacgat
    13021 tgttcgtaac attgcaccga ccaccacctg tcatcagtag tttttttcct gcttttttgt
    13081 ttttttcgat caagagtacg cgtgcgcctt gttcagctgc actgatcgct gccatcatcc
    13141 cgcttgttcc agcgccgact acgatcacat catacagttt ttccattccc tcacctcaag
    13201 gaaagtgtag catatctgaa gaaagaaaac agaaaaaaat gttttgagcc aaaagagaaa
    13261 atgagctaaa atgagggaag ataagcaaag gagcgtggaa cgatgacaaa cgcaacagaa
    13321 tatgttcaag caattcaaga aaagctacat caaaaggatc aagggcaaga agaattttta
    13381 caagcaatcg atgaattcat gcccacggtt atgtcttttt tggacactca tcccgaatat
    13441 atcgaaaaga atatcttaga acttttaacg gaaccagaaa gagttattca atttcgagtg
    13501 ccttggcaag atgatgaagg aaactggcga gtaaatagag ggtaccgtat ccaatacaac
    13561 tcagctatcg gcccttataa aggtggattg cgcttccatc ctagtgtcaa tctaagtatc
    13621 ttgaaatttt tggcttttga acagattttc aagaatagtt taaccggtct gccaatcggt
    13681 ggaggaaaag gcggaagtga cttcgatcct aaaggaaaat ctgataatga gatcatgcgt
    13741 ttttgtcaaa gtttcatgct ggaattgtca aaacatatcg gcccatcgat tgatgttcct
    13801 gcgggagata tcggtgtagg agctagagaa atcggctatt tgttcggcga atataaacga
    13861 ttgaaaaaat atgatactgg tgtgttgaca ggtaaacctt tggatttttg gggaagtaag
    13921 atacgtacgg aagctactgg ttatggatta gtgtattatg tcaaacatct attgaacgaa
    13981 gaaaaagatt catttaatca aaaaacagtt tttgtttccg gaagcgggaa tgtagccatt
    14041 tatgcaatcg aaaaagtgca agagctaggc gggaaagttg ttacatgttc agattctagc
    14101 gggtacatct atgatccaga aggaatagat gtgaaactat tgaaagaagt caaagaaatc
    14161 aatagacaac gtttgacgga atacgcaaac caacgtcctt ctgctattta tcatgctgga
    14221 gaatctgttt ggacattgaa agaaaaagcg gatatcgctt tgccatgtgc tactcaaaat
    14281 gaaatagacg aaagcttggc cgaaatctta gtagaaaatg ggatcaaaat cgtagcagaa
    14341 ggcgctaata tgccatgtac agtaaaagct gtttctgttc ttcatgaagc aggcgtgtgg
    14401 tattgtccag gaaaagctgc taatgctgga ggagtagcag tatctgcttt agaaatgagt
    14461 cagaacgtcc agcgattgac ttgggaaaga gagcaagtgg atgagcagct ggatcaaatc
    14521 atggaaacga tttaccatac gtgtcgccaa acagcagccg agtacggcga cgaaaaaaat
    14581 ctgcttcttg gagcaaatgt tgctggattt gaaaaagttg ccaaagcaat ggcagcagaa
    14641 ggtttagttt gaaaaatgaa actttcttgg aaatattgtc agcattttgt ttatttgctt
    14701 gaaaaataat aaaaaaaaac gtaaagtatt acctgtagag taaacctata aggaggagaa
    14761 caaatggcac acattcattt tgactattca aaagtagcac cgtttgtcaa cgaacaagag
    14821 ttagggtaca tgcaaagcca agtaacagca gcccacaacg aattacggga aggtactggc
    14881 gcaggaagcg acttccgcgg ctggatcgat ttaccaacga actatgacaa agaagaattt
    14941 gcacgtatca aagaagcagc taaaaaaatc caatctgatt ctgaagtatt agttgttatc
    15001 ggtatcggtg gttcttattt aggcgctcgt gcagcgatcg acttcttgaa tcacacattc
    15061 ttcaatttat tagatagcaa cacaagaaaa gcaccacaaa tcttctttgc cggaaattca
    15121 atcagctcaa catatattgc tgacttgatc gaagtaatcg gtgatcgcga cttttcagta
    15181 aacgttattt caaaatctgg aacaactact gaaccagcga ttgcgttccg tgtattcaaa
    15241 gagcttcttg ttaagaaata tggacaagaa gaagcaaaca aacgtatcta cgctacaaca
    15301 gataaagcaa aaggtgcagt taaagtagaa gcagatgcag aaggttggga aactttcgtg
    15361 attcctgatg acatcggcgg acgtttctct gtattaactg ctgttggttt gttaccaatc
    15421 gctgttagtg gtgcagatat cgatgcattg atgcaaggtg ccgctgatgc aagcaaagca
    15481 tactcaagcg acaaactaga agaaaacgaa gcttaccaat acgcagcaat gcgcaacatt
    15541 ctttaccgta aaggcaaagt aactgagttg ttgatcaact atgaaccagg aatgcaatac
    15601 ttctcagaat ggtggaaaca attgtatggt gaatctgaag gaaaagacca aaaaggcatc
    15661 tatccttcaa gcgcaaactt ctcaactgat ttgcactctt taggacaatt catccaagaa
    15721 ggacgtcgca atatttttga aacagttgtg aaggttgaaa aacctcgtaa atcaatcaca
    15781 attcctgaac aagcagaaga tttagacggt ctaggctact tgcaaggaaa agaagtggac
    15841 ttcgtaaata caaaagcatt tgaaggaaca ttgcttgctc atacagacgg tgacgtgcct
    15901 aacttgctag tcaaaattcc agaaatggat gcttatacat tgggttatac aatgtacttc
    15961 tttgaaattg ctgtaggtat ttcaggttac ttgaatggaa tcaacccatt cgaccaacca
    16021 ggtgtagaag cttacaagaa aaacatgttc gcacttctag gcaaaccagg ctttgaagat
    16081 cttgctaaag aattgaacga acgcctgtaa gataagggtt tttcgctaga tttaaataat
    16141 cagtgataga aacatgttcg ctttaaaatg aaaactggta cccatttagg tactcaaaca
    16201 tactgcatcg tttattcaga ataaggggct gtgacaagac gtttgtcaca gtcccttgtt
    16261 tgtatagaaa tcgattgatt gccttgctct ttagtttgtt ttaggacttt aagaacagtg
    16321 aggctgactt cgaaggttaa ttctgttttt ttgttttcta gaatcagtaa gcaaaaaagc
    16381 actcacttcg agtgcttttt tgcgatatat tcttagctta agataggcct aatttttctt
    16441 ttacagatga gggtacctta tcgctggtaa ttactttttt ctctatgatc cgcttttcgc
    16501 ttgctttcaa ctgcaagatc gttccttcgg ggtaagcaga accattggca gagtcaccag
    16561 aagtaatgac atcgaaatct aactgtcttt tttcaccgtt ttcagcataa gcatctacgt
    16621 tgtatttaaa acctcttcca attacttccc cattatctgc tttgatatca gtttctgcag
    16681 gcattggagc tttgactacc gcataataat ctttacctac ataagtagac tgatagtagt
    16741 cccaaccttt ccatcctata aaagcaatca atatgataac gatcaaccct aaaatttttt
    16801 tcatgaaaga tctccttttt tatttttatt atacccgtgg tgctagttaa ttttaatatc
    16861 ctaaagaata tctcaaagtt ccttcaaaac gt
//
LOCUS       DACCCS010000060        16687 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.061, whole genome shotgun sequence.
ACCESSION   DACCCS010000060 DACCCS010000000
VERSION     DACCCS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.061"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..189
                     /locus_tag="IVK82_002146"
     CDS             <1..189
                     /locus_tag="IVK82_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6393191.1"
                     /translation="NSNGILRRNGLPKSMDFREVNQTFISSVSNQRNYIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            326..1264
                     /locus_tag="IVK82_002147"
     CDS             326..1264
                     /locus_tag="IVK82_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP6393192.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            1279..2109
                     /locus_tag="IVK82_002148"
     CDS             1279..2109
                     /locus_tag="IVK82_002148"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP6393193.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLLVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            2153..3178
                     /locus_tag="IVK82_002149"
     CDS             2153..3178
                     /locus_tag="IVK82_002149"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP6393194.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNHYFRIYTNPDVVGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            3193..4131
                     /gene="galU"
                     /locus_tag="IVK82_002150"
     CDS             3193..4131
                     /gene="galU"
                     /locus_tag="IVK82_002150"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP6393195.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKIELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            4257..4697
                     /locus_tag="IVK82_002151"
     CDS             4257..4697
                     /locus_tag="IVK82_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP6393196.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIIRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            4700..5302
                     /locus_tag="IVK82_002152"
     CDS             4700..5302
                     /locus_tag="IVK82_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP6393197.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSAELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(5364..6467)
                     /locus_tag="IVK82_002153"
     CDS             complement(5364..6467)
                     /locus_tag="IVK82_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP6393198.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGADSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVVWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            6677..7678
                     /gene="ccpA"
                     /locus_tag="IVK82_002154"
     CDS             6677..7678
                     /gene="ccpA"
                     /locus_tag="IVK82_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP6393199.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            8032..8427
                     /locus_tag="IVK82_002155"
     CDS             8032..8427
                     /locus_tag="IVK82_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393200.1"
                     /translation="MPKKRRKTIVIILTILAIGIGGKFYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGCYGALVTMTNNEGKSVYFDYFFSKQMNEISSY
                     GVENEEIQNEGVTTPKIRVIYSNGEAEEV"
     gene            8424..8678
                     /locus_tag="IVK82_002156"
     CDS             8424..8678
                     /locus_tag="IVK82_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393201.1"
                     /translation="MTVKDEEYRYISEHVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKDSLDGMQAMAAAPIVHSKLEKNFKNKKIPTNFRVLK"
     gene            8721..9116
                     /locus_tag="IVK82_002157"
     CDS             8721..9116
                     /locus_tag="IVK82_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393202.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGAYRMFVTMINNHDESVYFTFGFWKERNELGDY
                     GVENKSIQKEGITVNKIRVIFSNNKEEYI"
     gene            9116..10171
                     /locus_tag="IVK82_002158"
     CDS             9116..10171
                     /locus_tag="IVK82_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP6393203.1"
                     /translation="MMADKQYSVISEAVYWLDPKHRDYDHTYKENSIKKIDSYNFKIL
                     KIKDSLDGMQAMGVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRPG
                     ASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALYV
                     AAEKQWKNVGLNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEVR
                     ISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQEL
                     NNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELEK
                     KWHGKKACGKHVLSPLR"
     gene            10168..10374
                     /locus_tag="IVK82_002159"
     CDS             10168..10374
                     /locus_tag="IVK82_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393204.1"
                     /translation="MTEQEIIEALASVVATKENLVDSAKEVYLLRINKARRMGEAFDT
                     LVKEIQDKINEIVTRDRELAQQFN"
     gene            10386..10709
                     /locus_tag="IVK82_002160"
     CDS             10386..10709
                     /locus_tag="IVK82_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393205.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            10702..10968
                     /locus_tag="IVK82_002161"
     CDS             10702..10968
                     /locus_tag="IVK82_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393206.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNHQ
                     MTAEEAAQEFSNRHVHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(11131..13476)
                     /locus_tag="IVK82_002162"
     CDS             complement(11131..13476)
                     /locus_tag="IVK82_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP6393207.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKVIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            13755..14657
                     /locus_tag="IVK82_002163"
     CDS             13755..14657
                     /locus_tag="IVK82_002163"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP6393208.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEGGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQAFDPEVFPELLAEEVTL"
     gene            14868..16643
                     /locus_tag="IVK82_002164"
     CDS             14868..16643
                     /locus_tag="IVK82_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP6393209.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSRRKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
BASE COUNT         5513 a         2987 c         3487 g         4700 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatt atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata taattaagaa tgcgttttct attacattgg taattaaata
      241 aaaacgtttt ttttaattgg tagtactttt ggctagacaa tgataaaatg gtagagaaca
      301 ctctaataga aatgaggtag catgaatgtc agaagttgta aaagttagcc aattagtaaa
      361 aaaattatct ttggaaatcg tcacaggcga cgagaaaagt ttagaccgcg tcatcgtgac
      421 tggggacatc tcacgcccag gactagagtt gaccggttat tttaattatt attctcataa
      481 tcgtttacag ctatttggca gtaaagaaat cagttttgcg gaacggatga tcccagaaga
      541 gcgtttgatc atcatgagaa gattatgtag tgaagatatg cccgcattta ttatttcaag
      601 aggattgccg gctccggaag aaatgatcca agcagccaat gaaaatggga tagctgtgct
      661 gcgttcgcct atctcaacgt ctcggctgct aggtgaatta tctagttatt tagatgggcg
      721 cttagcccct cgaacaagtg tccacggtgt gttagtcgat gtctacggat taggtgtact
      781 aattcaaggg gacagtggga tagggaaaag tgaaacggcg ttggaactga ttaagcgcgg
      841 acatcgattg atcgcagacg atcgagtaga tgtctatcag caagatgaac tgacggtaat
      901 cggagaacct cctaaaatcc tggaacatct gattgagatt cgagggatcg ggatcataga
      961 tgtaatgaat cttttcggtg cgagtgcggt acgaggctct atgcaagtcc agttagctgt
     1021 ttatctagaa gcatgggcga aagataagaa atacgaccgt ttgggttctg aagacacgac
     1081 cgttgaaatc gcggaagtgg gcattccaca aataaaaatc cctgtcaaaa caggacgaaa
     1141 cgttgcaatc atcattgaag tagccgcgat gaatttccgc gcgcgtacaa tgggctttga
     1201 cgcaacgaaa acatttgaag aaagattaag tcgcttgatt gaagaaaact caggcaatga
     1261 ctagcaagga gtgacaatat gttagcacaa atcaatcgag ttgcttttga gcttgtagga
     1321 attcctatct actggtatgc attgattatc gtatcaggaa tcattattgc catgtggctc
     1381 agcagcagag aagctgttcg ggtaggatta aaagcagatg acgtgacaga ttttatgttg
     1441 tggggattgc cgatttccat tattggtgcc cgtttgtatt atgttttatt cgatcttccc
     1501 caatatatag ctgatccaat ccaaatattc aatatccgtt caggaggatt agcgatttac
     1561 gggggattga ttgcaggtgc aatcacactg tactttttta ccaaatatca ttttatttct
     1621 ttgtggacct ttttagatat cgctgcacca agtgtattac tggcgcaggc aatcggacga
     1681 tggggaaatt tcatgaacca cgaagcgtat ggtccagaca cgacacgaag tttcttagaa
     1741 agtctgcatt taccgcaatt catcattgat aacatgtata tcgaagggat ctatcgacaa
     1801 ccaacctttc tttatgaatc actttggagt ttgattggat ttattctgct tttggttttg
     1861 cggaagaaac cgcatctttt gaaaaaagga gaagtccttt tatgttatct aatgtggtat
     1921 agtttcggac gtttctttat cgaaggatta cgcacagaca gcctttactt tttaggtgtg
     1981 atccgcattt ctcaattatt gtctgctgtt ttacttgtcg gcacattgat tctgtttatt
     2041 tggcgtagaa aaaaaggaaa tttaccggat tatgaccgct cattaggaaa aaatcaacaa
     2101 attatttgat ttttagagcg ttaaaaatga aaaaaatttc aggagtggta aaatgaatta
     2161 tagagtagcg gttttaggac caggctcttg ggggactgct ttagcacaag tcctcgctga
     2221 aaacggacat gacgttcgga tttggggaca tcgtgcttca caagtagaag aaatcaacac
     2281 ccaacacacc aatcaacgtt atttaccaga tcatcatcta cccgattcca tccgagcatt
     2341 caccgatatg gaagatgcag taaaagatgc agatgcggta ctattcgtta tcccgacgaa
     2401 agcgatacgt tctgttgcaa aagaactagt tgccaaattg aatacgaagc cagtgatcat
     2461 ccacgcaagt aagggattag aacagggaac gcataaaaga atctctgaaa tcttgttaga
     2521 ggaaatcccg acagaaaaaa gaaaagctgt tgctgttctt tctgggccaa gccatgcgga
     2581 agaagtagca aagcatgaca ttacgacgat cacagcggca agcacagatg caaaagcagc
     2641 aaaatatgta caagagctgt tcatgaacca ttattttcga atctatacta accctgatgt
     2701 ggttggtgta gagatggggg cagctttgaa aaatattatt gccattggag ctggtgctat
     2761 tcacggctta gggtttggcg ataatgcaaa agcggcaatc atgacgcgcg gattagctga
     2821 gatcagccgt ttaggtgtag caatgggtgc caacccgctg acctttattg gcttaagcgg
     2881 agtaggcgat ctagtcgtta cttgcacaag tgttcattcc agaaactggc gcgcaggcaa
     2941 cttgttagga caaggacagc ctctagaaga ggtgctagcc aatatgggaa tggtcgttga
     3001 aggtgtgaat actactcaag cagcgatgga actagcccaa atgatggaaa tcgaaatgcc
     3061 catcactcaa acaatctatg aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa
     3121 agaaatcatg ttacgtgatg ggaaaaaaga aaatgaattc tcattaacaa ttttttgaag
     3181 gagcgaatgt atatgaaagt aaaaaaagca gttattccag cagcaggatt aggaacaaga
     3241 tttttaccag caacaaaggc gatggcaaag gaaatgttgc caatcgtgga caaaccaacg
     3301 atccaattca tcgttgaaga agcgttgaaa tcaggaatcg aagacatctt gatcgttaca
     3361 ggaaaagcta aacgaccaat cgaagatcac tttgattcaa atatcgaatt agaaacaaac
     3421 ctaagtgaaa aaggaaaaat cgaattattg aaattagtag aagaaacgac agatgtcaac
     3481 ttgcatttca tccgtcaatc tcatccaaag gggttaggac acgccgttct tcaagcaaga
     3541 gcctttattg gaaatgaacc gtttgtcgtt atgctaggcg atgatatcat ggaagacgaa
     3601 gtaccattga cacagcaatt gatcgatgac tatgaacgta cacacgcttc tacgattgcc
     3661 gttatgcaag ttcctcacga tgaaacttca aaatacggaa ttatcaaccc aggggaagta
     3721 ctggaagatg ggttatacaa tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca
     3781 ccaagtgatt tagctattat tgggcgttac ttattgactc cagaaatctt tgatgtatta
     3841 tcagaacaac aaccaggtgc tggaaatgag atccaactaa ctgacgcaat cgatacgttg
     3901 aataaaacac aacgcgtatt tgcccgtaaa ttcacaggta aacgttatga cgttggtgac
     3961 aaatttggtt ttatgaaaac aagtattgaa tatggtctga cacatccaga agtaggggca
     4021 cctttgcgtg aatacatcat gtctttaggt aaaacattag aagcagaagg aagcaagatc
     4081 gatcataatc caaaaggaat aacagaaatc aaagaagaaa agaaaaaata atcatttttt
     4141 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     4201 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     4261 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     4321 tcattcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     4381 aagcaaatac gacaattgag atcgtcacaa aagacgtaga tcttttatct agacaagtag
     4441 aaggcttgct ggtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     4501 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     4561 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     4621 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     4681 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     4741 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     4801 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     4861 ggcaaaagaa aaatcagctg agttaacagg cattgcaaaa gaaaaagcgc aagatctttc
     4921 tcaacaagca ggggatctag caggtaatgt taagcaaaag gtaagtgaat cagcagagga
     4981 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     5041 taatcgtttc aaaaaaacag cagaagatct gaaaaaacca gcagacgaat taggtagtat
     5101 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     5161 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     5221 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     5281 agattcgact accaatcact aagaatactc atttctagaa taaaaaggtg agagcggacc
     5341 agctcccacc ttttttattt tcattactct aaaacaagta attcttttgg tgtagtcgtg
     5401 aatggcacac aaccatcact tgtcacatac acacaatctt cgatacgtac ccctactttt
     5461 tcaggaatat agattcctgg ttcaagagag aagcacatcc cttcttcgat cactaagtcg
     5521 tttccttcta caagagaagg gaattcgtgg acggttgtcc caattccgtg acctaaacgg
     5581 tgattgaaat attcgccgta gccatagctg ttgattacgt ttcgtgcgat ttgatcaagt
     5641 tcacctgctg tcacacctgg gcgaactgcc tcttgtgcgg caagctgtgc ttcaagagtg
     5701 atgttgtaga ttttttcttg gaattctgtt ggtttttgat aagcaactgt acgagtagca
     5761 tcactgcaat agccattcca aacaacgcca agatcgaata acactagttc atttggtgaa
     5821 accttcgtgc ttcctggtgt accatgaggg cttgcagcat ttgctcctgc aaggactaag
     5881 gtgtcgaaag acatttgtga aacaccttgt ttttttaatt ggtattcgat ttctgcgata
     5941 attgcttgtt cagtcacgcc ttcttttact gttttgaagc caatttcaaa ggcaacatcg
     6001 gcccatttac cagcttcgat cagtttgtct ttttcttcta ccgtcttgat cagttgcaga
     6061 cgctggataa tagaagttag atcagtgtaa gaatctgcac ctgtcaagat atcagagata
     6121 gcatcgaagc gatcgacagt cagagcattc ttttcaatcc ctacattacc aggttgccca
     6181 tagcgtgcgc gaagctcttt tccgatgatc tcccaagggt tttcgctgtc taagtagcca
     6241 cgtacatcgt acgcccagct gctgttttga gcatcttcaa cttccagagc aggagtgaaa
     6301 aggaaaggat cttgttcaag agggataaaa agagctagta cacgctcgtg aggatcactt
     6361 tcatatccgg aaaagtaagc gatgtggcca gggtcgctga tataagccaa ttctacttgg
     6421 tgttgtgcca tccattgacg tagttcattg atttttacgt tattcatttg gcaacctact
     6481 ttctaaatta attttgtatt caataattgt accactgatt gattaaaaga ccaacgttct
     6541 taaaactgaa aaaagtaaaa attttcatga aaacagagat aaaacggttg caattgttga
     6601 gattatttga tatcctattt tagaaatgta agacgtattt tcagaaaaca tataaaatat
     6661 gaataggaga acacaaatgg aaaagcaaac aatcactatc tatgatgtag cgagagaggc
     6721 aaatgtttcg atggctactg tttcacgtgt cgtaaatggc aatccaaacg tcaaaccagc
     6781 aacaagaaaa aaagtcttgg aagttatcga tcgcttggat tatcgtccaa atgcagtcgc
     6841 acgtggcttg gcaagtaaga aaacaacaac tgttggggta atcattcctg atgtcagcaa
     6901 tatgttcttt gcctctttag cacgtggtat cgacgatgta gcaacaatgt acaaatacaa
     6961 tatcatctta gcaaactcag atggcaacga tcaaaaagaa gtcaatgtat tgaataattt
     7021 attggctaag caagtggacg gcgtgatctt tatgggtcac catatcacag acgaaatccg
     7081 tggagaattt tcacgttcaa aaacacctgt tgtactagct ggttccattg atccagatga
     7141 acaagtaggt agcgtaaaca ttgattatgt atcagcaaca aaagatgcag taaataaatt
     7201 agcaaaaagc ggcaataaaa aaattgcttt tgtcagcggt gcgctgattg atcctatcaa
     7261 tggtcaaaac cgtctaaaag gctataaaga agcattaagc gaaaacaatc taccatatag
     7321 cgaaggatta atctttgagg cacaatacaa ctttaaagat ggtctgtctc tagttgaccg
     7381 gatccataat agcggtgcaa cagctgtcta tgtttcagac gatgaattag ccattggtat
     7441 cttggatggt ttattagacc gtggcgtaaa agtaccagaa gaattcgaaa tcatcacaag
     7501 caacaattct ttactgacag aagtagctcg tcctcgtttg acaagtatca cacaaccatt
     7561 atacgattta ggagcggtat ccatgcgcct attgacgaaa atgatgaata aagaagaagt
     7621 cgaagaaaaa acgattatct tgccttatgg aatcgaagaa aaaggatcaa ctaaataata
     7681 aaagatcaat catttagagg ttgttccaaa agtggccaac ttctggaaca acgtttattc
     7741 gagaaaaggg gttatgacaa aattttgtca taaccccttt tttttgtgac tttaaaaaaa
     7801 gagaaaatgg cggaaacaaa ccttacgtcc atcgtatacg acacaatctg tgcgtagatg
     7861 ctacattttg ttcactacta tcaaaattac tttaaaatga aaacgtgata aaaaagaaag
     7921 tagaaaagta gaaaagtaac tctaaaaaat ccaaaaaaat ccaggctcga ttgcgtactt
     7981 atcttaaagg agaaacaatc catcatacat aacccgaaag gagacttcta tttgcctaag
     8041 aaaagacgaa agaccatagt catcattttg accattcttg ctataggaat cggaggaaaa
     8101 ttttacatgg acaaaagaga agcacaaaga cagaaagatt tactcgcagt agaaaaacaa
     8161 tccgtcaaag ttctgaaaaa tacctttgca gatatcaggg aagtgaaggt agaagaattt
     8221 aaaaaaaacc ctgttactgg atgttatggt gcacttgtga caatgacaaa taatgaaggg
     8281 aaatctgttt attttgatta ttttttttca aaacaaatga atgaaattag cagttatggt
     8341 gtagaaaatg aagaaataca aaacgaaggg gttactactc ctaaaataag agttatttat
     8401 tctaatggag aggcggagga agtgtgacag taaaagacga agagtataga tatataagtg
     8461 aacatgttta ttggctagat ccaaaacatc ctcaatatag taaaagttac aaagaaggcg
     8521 ccattaaaaa ctttggagaa acaaaattcc aaatcctaaa aatcaaagac tcattggatg
     8581 gcatgcaggc gatggctgca gcgccaatcg tccatagcaa attggagaaa aatttcaaaa
     8641 ataaaaaaat tcctacaaac tttcgcgtac ttaaataatg aaggtatcaa tacttacata
     8701 aaaagaaagg aggcacgttc ttgtctcgga aaataagaaa actcagctta gccattttat
     8761 taatcgtcat attaggaatc ggagggaaag tctatatgga caaaagagaa gcacaaagac
     8821 agaaagattt actcgcagta gaaaaacagt cagtcaaagt tttgaaaaat acttttgcgg
     8881 atatcaagga agtgaagatt gaaaaatctg ctcgaaatga aatgacggga gcatatcgta
     8941 tgtttgtgac aatgataaat aatcacgatg agtctgttta ttttacgttt ggattttgga
     9001 aagaacgaaa tgaattagga gattatggcg tagaaaataa aagtatacaa aaagagggga
     9061 ttacagtaaa taaaatcaga gtaatttttt ctaataataa ggaggaatat atttaatgat
     9121 ggcagacaaa caatacagtg ttattagtga ggctgtttac tggctagatc ctaaacacag
     9181 agactatgat catacttata aagaaaattc tattaaaaaa attgatagtt ataattttaa
     9241 aatcctaaaa atcaaagact cattggacgg catgcaggcg atgggtgtag cgccaatcaa
     9301 gaacggtaaa gtggatcttt ctcaagtagt gattgctttt gcaggaacga acttaagtga
     9361 ttggaaagat ttggaaacgg atgcaaggag tatcatgaat ctctatggga atagaccagg
     9421 agcaagtttt tctgaaaaag caacaacaaa caagatttta tccgttagtg gattgaaaac
     9481 agcggggcaa atcaattcag cgattgcatt tgctaatgaa gtaaaaaagg aatatggaga
     9541 agctgctatt tctactaccg gccattcatt aggtggattc ttagcattat atgtagcagc
     9601 agagaaacaa tggaaaaatg tcggcttaaa cgggccggac ccgtatgatc tcttatcacc
     9661 agaagcaaaa aaatgggtgg aagaaaatcc cggtatgctc accaattacc gaaatcacgc
     9721 cgattttatc ggtaattttg gtggtaatgg cacaggtgct gaagttcgga tcagtatgga
     9781 aacagcattc ttgaatctgc ttgcttccca cagtttaacc tcttggaagt ttgatgaaaa
     9841 tggaaagctg attattcctg aaaatgaaaa taatgttaaa gctcgaaaac aacagaaaga
     9901 aaaccaagcg acccagttgt tttcctatca gttgcaagaa ctgaacaaca tacgtaaaaa
     9961 actacgatta agtggcggcc ggctgacagt taatgaacaa atttacttag atgacacaca
    10021 agctaggtta gtagtggaaa agttatcagc aaaaatgaaa agcgctatgg aagaagttat
    10081 ccgcatcaat caaagaggaa tttttgagct agagaaaaaa tggcatggca agaaggcttg
    10141 cggcaagcac gtgttgtcgc cccttcgttg actgaacagg aaatcataga agcattagct
    10201 agtgttgtcg ccacaaaaga gaacttagtc gattctgcga aagaagtcta tctgctgcga
    10261 atcaataagg caagacgtat gggggaggcc tttgatacat tggtgaagga gatccaagac
    10321 aaaatcaatg aaatcgtgac acgagatcga gaattagcac agcaattcaa ttaaaggaga
    10381 agtagatgaa actagaagaa ttggaaagag attatcgaag tgaacgaaga caattagaag
    10441 ctcaagaaga tgaattgatc cactttaaga atcaaggatt acgattgatg gagcaagagg
    10501 agcagcgact gcatcatgta ttaaagaact tagctttaga taatgagcct ctgtttcaag
    10561 caagacgaag ttattcgaat tatcaggagc attatcaaca agccctacaa gtagaacgac
    10621 gaaaactcta tcagcagcaa gacgaactag aacgttccta tcgtcaaaaa atcagacaaa
    10681 taactatgga gaaatcatct tatgaatgaa tttttaatgc aattactaaa agaattagag
    10741 aaaaatctac atcgatcaaa tgtacgttca gttggtatgg ggatatctca gcaaatttgg
    10801 gcagaaaaac gattgacaga tcaaaaccac caaatgactg cagaagaagc tgctcaagaa
    10861 ttttctaacc gacatgtaca cttgaaagaa tcagtaaaag ggcaatttgc agaaaaacta
    10921 accgaattat tccaaagaca agaaaagaaa ttaaaacagt ttcgttagta aagttatata
    10981 gaaaaatttt gtgaaaatgg cttttcacat ttttacataa atttgtctta atatcgtaga
    11041 tgcaagcttt tgaacaccat ttattcggaa aaagggagct gtgacaagat ttttgtcgca
    11101 gctcccttta attctgatta atctattaga tcaatcatct ttatcgtctg attctttggt
    11161 ctctggattc gctctagcat aagctgaagc ggtgttccag taatctgtat agttttcatc
    11221 agtaccgcca acgccgaatt taaaggcgct tttcttcgga ccattttttg cccaaagtga
    11281 ttccactgtt tttcctggcg tttggataga ttttttattg acagtaactt ttccagctgt
    11341 ttgtcctgtg aacccagata cttttttctt catcacatca gatgataagt caaatttctc
    11401 atcagtgccg aaaatctcag ggtttgcttg gtaagcttga ttgattagat tggcaagata
    11461 attagccgaa cgtatccttg cttggctgtc cattccagta ttgtcatcgt gtcctgccca
    11521 gctactgata gtgatagacg gagtagagac gatcagccat gaatctctgt attcgttggt
    11581 agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc ttgaaatgac
    11641 atctttgaat ggtgtagtga ttttttcatt gatcacactg cgcatcatgt cattcatgat
    11701 cgaggccgtt gcttcggaat aaatacggac agaattactt tcatgtttat aaatcacatt
    11761 acctgcatta tctgtgatgg aatcaatgat atagccttct tcgtagacgc caccattagc
    11821 taaggcctgg aatccatttg tctgttgaag cgtcgttaca tctactgtcc ctaaaggagc
    11881 agattcaacg ccccaattat catttgctgg gtagttcatc ttcgccaagt agtgctcgta
    11941 agcatattgt ttcgatccac ccgaattcaa tacgtcttgg tataagtgat aagctgggat
    12001 attgttggac cattccagtg actcacggac ggtttggaag gtgttcgacc cttcattggt
    12061 cgcattgacg attttctcgc cagcattttc tccttcttgc caagtcgttg cataatctga
    12121 tacacgggat tctgatccga caagtcccat atcgattgca ggaccgtaaa caagaaccgg
    12181 tttgatggat gaaccagctt gacgttctgt atcaaaggcg tggttatttt gattctggct
    12241 gtaattccgg ccacccacga atccatagat acgtccagtc ctattgtcca tcaatacatt
    12301 tccagtctcc acttggctac cagcaccatc atctaacata tagccgtaat tttctacgcc
    12361 ggtttgcaat gcagcataga tatctttatc gatcgtcgaa tggatcgtat atcctttgtt
    12421 ttggatcgtt tgctgtgctt tttgataata agcttcactg accgatgaat ctgacatatc
    12481 agcattatct ttttctgcca attgttgtgc aataatcctt gttgcttcgt tcatcacggt
    12541 ataatacaag aattctctgt cagtctgttc cgcgatttgt tgtgggagaa agtcttttgt
    12601 aagatcatag gctttcgctt cttcgtattg ttctttcgta atttgtcctt cacggtacat
    12661 actgaataaa acaaaatctt ttctggcaag accggcagaa agatcttctt ttagcgcgcc
    12721 tgtattcgtg tagggactat acgtgatagg gctttgaggc aaaccagcga tataagcagc
    12781 ttgtggcaga gtcacatctt ttgcatctac accaaagatt cctcttgcag cttcctggac
    12841 tccagcaata ttttgcccct tattattccg accaaatggc gagacattca aatacgtagc
    12901 aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaacga tctcgtttgc
    12961 tttacgtttg aatgttgttt catcggttag gatctgctgt ttgactaatt gctgtgtcaa
    13021 agtggatcct ccagacgagc cgatgccagt agcttctgaa actaatgctc gtaaaacagc
    13081 tttagggaca taaccttggt gtttatcgaa gtattcatct tctgtactga taatcgcggt
    13141 tttcaataaa ggcgaaatct tatcagatga aatagtcgtc cgcatcagat ctgtctggat
    13201 ttttgatatc tctgtattat ctgcataaac aatctttgac gtttccgtaa tgtttcctag
    13261 ttctgtctgc agttcttttt tagtcggcaa ttgtgtgtct tcgacaagat aggcaaagta
    13321 gccagcacct attccgatac ctagagctgc gaacaacaaa agaagaacag taataaaaac
    13381 agtcaatgat tgaaacactc taaaaataac cttaggaaca aaccattttt ctttttttct
    13441 tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa ctaatggttt
    13501 tatttcgcta attatagcaa ataataagac aaaaaaacag acaaggctgt ttttgacagg
    13561 aatttctttt tcgaatgaga taaaacgtga ggaaatgatc ttttttatga aagagttctt
    13621 aagcttttct ttagagaaag tttcagttac tatggagaac agggcaaaga aaaacaaacc
    13681 attttctttg tacatcatct taccaatata taggaattgt tcgaaacaat tgcctttcaa
    13741 ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag aaaatgctgt
    13801 taggcgcggc agaagaagca gccagttacg gatcttcgac ctttatgatc tatacaggcg
    13861 ctcctcaaaa tactagaaga aaagctatcg aagagatgaa tattgatgcc ggaaaagcat
    13921 ttatggcaga aaacggacta acaaatatcg tcgttcatgc gccctatatc attaatttgg
    13981 gaaatacgat caaaccagaa atgtttccat ttgctgtaca atttctgaga gaagagattg
    14041 ttcgtgcgga agctttaggt gctactcaaa tcacccttca cccaggcgct catgtaggag
    14101 caggggcaga aggtgggatt gctagaatta ttgagggttt gaatgaagtt ttgacaaaag
    14161 aacagacggc acagatcgct ttggagacga tggcagggaa aggtacggaa atcggtcgaa
    14221 catttgagga actagcgcaa atcatcgatg gagtaaaatt gaatgaaaaa ttatccgtta
    14281 cttttgatac atgtcatacg aatgatgccg gatataatat aaaagaagat ttctcaggtg
    14341 tgatggaaga gtttgataag gtcatcggat tagatcggct taaagtactc catatcaacg
    14401 attctaaaaa tccacaaggt tctcataaag atcggcatgc caatatcggt tttggaacaa
    14461 ttggatttga cacattgaac aaaatcgtcc atgatccaat gtttgacaac atttcgaaaa
    14521 tcttagaaac tccatatgtc ggtaaagata aaaagaaagc ttacgcacca tatggaaaag
    14581 aaattgccat gttcaaagcg caagcattcg atcccgaagt gtttcctgag ttattagcag
    14641 aagaagtcac actttaagtt ttctatctga cagcaacaaa aaaggattgg gcagtctgcc
    14701 ttgtcctttt ttgtttaaga aacaaaggag tgctttttgc agtcgttttc gccatgctcc
    14761 tttcgccccc tctttttaaa ttcagacaaa cagggtatac tgatagaaaa gtaaaaaacg
    14821 aaaagattca caagcaagta gcaaaacgtg attgaaagga cgaaaagatg ctgaacactt
    14881 taaaaccctt gatcaaccaa ctaaaaaaga aacatacata ctttattctt ggcatagccg
    14941 tattatttac aattattcgg ttgtatttat atagtaaagc aatctatgga acagatatgt
    15001 cggcaaccta tgatgatcag ctgctgattc actatgctca aaatattttg gatggtaaat
    15061 ggctagggga gtacacgact acgacacttt caaaagggat ctcctacagc ttgtttttag
    15121 tacttgcaca taaactgcat ctttcttatg cagttttgtt cggcatgttc agtattcttg
    15181 ccagcatatt ggttgcacta gcgtttaaac cgatcgtgaa gaacaagtat cttcttttaa
    15241 gtatctattt ttattttttg ttttctccga ttagttttac acatgagtat tccactcgaa
    15301 cgtatcgtaa tgctctcgtg atcccggctg ttgagatcat tatcgcttgt ttactagcta
    15361 tttattaccg taaatatagt tcccgcagaa aattgctgcc gtggttttta gggttagctg
    15421 tttcttttcc tttcttttgg ttcattcgcg aagattccat ctggcttctt ccttttactg
    15481 tagtcgcgtt aggcatcagt ttcgttcaga tcttgctagg acaagacttt tctttcaaag
    15541 aagcaaagaa gttaaaagaa cgaccatttc tcctttcgaa acaagaatgg ctgaaacttt
    15601 cattgttttt actccctcta gtcggtttct ccatcgtcta tcagtggatc gaatataaaa
    15661 acgagacaac atatggtatt gccaccatca atgaccgttc gagtggtgaa tttggacgtt
    15721 tgatgaaaca gctgatccga atagatgacg aaacgaactt aaataaagaa aacagtgaga
    15781 tatgggtctc gcatgctgca ttaaataaag cattagacgc atctcctaca tttcatactg
    15841 tttctactcg tattgacgaa ttgtatacga ctcatgcgtg gacccaaggc ggaaaagtaa
    15901 aagaattacc aggagatatc attttttggg cattgcgaga tgtggtaagc gaatctggtt
    15961 attaccaaaa caatgcaaaa gaaaccaatg atttttggaa aaaagtcaat caagaattag
    16021 aagaagccta tcaaaaggga actatcaaaa agaaaaaaga attctatctg atgggatcag
    16081 gggatggaaa gacgatgaat gatctgccaa tcgtatttgc ttttctgaaa tcaggatatg
    16141 cctatgcatg gcgatacaaa gatttccatc aaggagggaa ttatagtttc gggacgcaag
    16201 aacaagtcga acaggcccaa gaattattga atcttccact aatggataat tggatcgata
    16261 gcagtgatcc gagaacacct gatttccagt tgacaaaaac agcaaaagtc gcgaatgtca
    16321 tgataaaaat ttaccaagtt ctggccattc ccgtgctgat tgcttcaata gcagggatat
    16381 tacttatgat actaggaacc gtatttgcaa aagctgatcg tgcctattat agttcctctt
    16441 tgattgtagt caccggatta atactgacac aattggtttt tcttttcggt gtctcgtggt
    16501 tttgttctta tgcgccagaa aaaaaggatt attttttaag tgtctacact ggcgcaggcg
    16561 tgccattaac acaattcatc gtagtaacaa gcatgctagt tttcagtaaa ttgaatctga
    16621 aaaaagcttt atcttctaaa taaaaaggga actgtgacaa aagtcttgtc gcagttcctt
    16681 tttccga
//
LOCUS       DACCCS010000061        16527 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.062, whole genome shotgun sequence.
ACCESSION   DACCCS010000061 DACCCS010000000
VERSION     DACCCS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.062"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(141..416)
                     /locus_tag="IVK82_002165"
     CDS             complement(141..416)
                     /locus_tag="IVK82_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP6393210.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(659..1969)
                     /locus_tag="IVK82_002166"
     CDS             complement(659..1969)
                     /locus_tag="IVK82_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP6393211.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="IVK82_002167"
     CDS             complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="IVK82_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP6393212.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3515..4195)
                     /locus_tag="IVK82_002168"
     CDS             complement(3515..4195)
                     /locus_tag="IVK82_002168"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP6393213.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4244..4879)
                     /locus_tag="IVK82_002169"
     CDS             complement(4244..4879)
                     /locus_tag="IVK82_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6393214.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4945..6348)
                     /locus_tag="IVK82_002170"
     CDS             complement(4945..6348)
                     /locus_tag="IVK82_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP6393215.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6335..7366)
                     /locus_tag="IVK82_002171"
     CDS             complement(6335..7366)
                     /locus_tag="IVK82_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393216.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7397..7618
                     /locus_tag="IVK82_002172"
     CDS             7397..7618
                     /locus_tag="IVK82_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP6393217.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7783..8520)
                     /locus_tag="IVK82_002173"
     CDS             complement(7783..8520)
                     /locus_tag="IVK82_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP6393218.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8673..10013)
                     /locus_tag="IVK82_002174"
     CDS             complement(8673..10013)
                     /locus_tag="IVK82_002174"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP6393219.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10250..11773)
                     /locus_tag="IVK82_002175"
     CDS             complement(10250..11773)
                     /locus_tag="IVK82_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP6393220.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12151..12762)
                     /locus_tag="IVK82_002176"
     CDS             complement(12151..12762)
                     /locus_tag="IVK82_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6393221.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12885..13010)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13092..13808)
                     /locus_tag="IVK82_002177"
     CDS             complement(13092..13808)
                     /locus_tag="IVK82_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP6393222.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="IVK82_002178"
     CDS             complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="IVK82_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP6393223.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14367..15164)
                     /locus_tag="IVK82_002179"
     CDS             complement(14367..15164)
                     /locus_tag="IVK82_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP6393224.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15134..15505)
                     /locus_tag="IVK82_002180"
     CDS             complement(15134..15505)
                     /locus_tag="IVK82_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP6393225.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="IVK82_002181"
     CDS             complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="IVK82_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP6393226.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4645 a         3577 c         2798 g         5507 t
ORIGIN      
        1 aaaaattaaa taaaatacat gtgttttcta tgtatgacat acggattcgg aatctctgtc
       61 tcttatttat tccttttctg ctaggaaata aaaagaaaag cacttgtagc cctagagcca
      121 caagcgcttt ttcaactgtc ttatttaaca gcgtctttta atgctttacc tggtttgaat
      181 gcaggtactt tgctagctgg aatttcgatt tcttcaccag tttgtgggtt acgacctttg
      241 cgagctgcgc gttcgcgaac ttcaaagtta ccaaaaccga ttaattgaac tttttcgccg
      301 ttagctaaag catcttgaat agttgaaaat acagcatcta ctgctgcagt tgcatctttt
      361 ttagttaaat cagtagctga agcaactttt tcgattaatt ctgctttgtt tgccatggat
      421 agattcacct cctccacaag agatatgata gaaatatcat ttagttaaaa ctttctgagt
      481 ggtccagaag tttaaaaaga aatattgatt aacttcaata ttctgttatc acgatatcat
      541 atgaaccgct taatagcaag gtttttacca attttcacat gcttttttta gtcatagaaa
      601 cgtaacaaac gaaactgtct ttttcttttg aaaaaaaaac gtcgatatgg taaaatgcct
      661 attttctcct acgagggata attcggatgg acgtcccttc aaaagtaaat gcttttctga
      721 tttgattttc taagaaacgt tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa
      781 cgacaaatgt aggtggtttg acagcgactt gtgtagcata aaaaatcttc aggcgttttc
      841 ctttatctgt tggtgttgga ttgattgcta cagcatccat aatgatatca ttcaacacag
      901 ccgaagagat acgaagattc tggttcatac ttacttgttc gatcatttca ggcagacgtt
      961 ccaaccgttg tttagtcact gcagaaacaa aaacgattgg agcgtaatca agataccgga
     1021 attcatcacg aatctcttgt tcgaaatcac gcattgtatt ggtttctttc tttacaagat
     1081 cccatttatt cacgacgata ataattccac gaccagcttc atgggcgtaa cccgctactc
     1141 gcttgtcttg ttcccgaatc ccttcttcag cattcagtac catcaaaacg atatcagaac
     1201 ggtcaatggc acgcattgca cgcatcacgc tgtatttttc agtattttcg tatacttttc
     1261 cgcgtttacg cataccagct gtatctatca ttaagaattt ctgaccactt tcagaaacga
     1321 agtgggtgtc gattgcgtct cgggtcgttc cttcaatatc cgaaacaatt acacgttctt
     1381 ctcctaaaat cgcattgatc aaagaggatt taccaacatt tgggcgacca atcaaactaa
     1441 acttaatcgt gtcgtcgtcc tcttcttcag tctcttcaga gaaatacttc actgcttcat
     1501 ctaaaatatc accaatccct agtccatggc ttcctgaaac aggataagga tcgcctagcc
     1561 ctaaggaata aaattcatag atatcgtttc tcatttctgg attatctact ttattgactg
     1621 ctaaaatcac tggtttatta cttctgtata aaattttcgc aaccatctca tctgcgtctg
     1681 tcacgccttc gcgtccgctg actacacaaa tgattacatc tgcttcttca atcgcgattt
     1741 ctgcttgatg cttgatctga tccataaaag gttcatcgcc aagatcaatt ccgcctgtgt
     1801 cgatcacgct gaattctcga cctaaccatt caccttttgc ataaatacga tcacgagtta
     1861 ctccaggagt atcttcgaca atcgagatac gctcaccagc gatgcggtta aaaatcgtag
     1921 acttaccgac gttcgggcgg ccaacgattg caattgttgg atttgccata ttatttcctc
     1981 ctcacaagtt tccaatctca cttagtttaa cgaatgcatt gccaactttc aagcatagat
     2041 tgtcaattaa acgaagtatt tttattcctt attgaaatac taatcgcttt tcaaaagaaa
     2101 aactgagaca aaagcagctg ctttcatctc agtcacagat acaaaatcaa ttatttttca
     2161 gaatcatcgg ttgctttttc ttctacttct tctttcaacg catcacctaa aatgtctccc
     2221 atagtaaatc cagtattttc ttctggtaat tcataagact caacattttt tggttcttct
     2281 tctgaagctg gtttttcttc taaagctttg atactcaatg caatacgatg ttcttctgga
     2341 tggatctcta atactttcac tttcacttcg tcaccttcat gaagcacttc atgtggtgtc
     2401 gcaatatgtt tatgtgaaat ttgagagata tggactagcc cttctactcc agggaacact
     2461 tcaacaaagg caccgaagct tgtcaaacgt ttaactgttc cgtctaatac tgtgccaact
     2521 ggagcttttg tatcgatttc tgaccaaggt ccaggctgtg tgtctttgat tgacaaggaa
     2581 acacgttctt gttcaggatt gatagacaac actttgacat ttacatcatc attgacagcc
     2641 aatacatcac ttggttttgc tacatgacta tgtgaaatct ctgagacatg gactagaccg
     2701 tcaattccgc ctaggtcgac aaatgcgcca aaatcagtaa gacgtgcaac acgtccactg
     2761 agaacatctc cctcatggat agttgaaaga atttcttttt tcttttcttc tctttcgttt
     2821 tccactaccg ctttatgcga taagatcaaa cgattttctg atggttcgat ttcgatgatt
     2881 ttaaagctca atgttttgcc tttatagtct gaaaagtctg caacaaaatg atcttccacc
     2941 attgatgcag ggacaaaacc acgtacaccg acgtcaacga ctaaaccgcc tttgacaaca
     3001 tttgtaacag gtgcttcgat gaccttgccc tcttggaaat ctctttcgat atcttcccaa
     3061 acttttttag catctaaacg gcgttttgaa agtaggtagc tgccgttttc tttatctttg
     3121 ccgatcgtac tgataacgac taaatctaat acatcaccga cttttactac ttcgctgata
     3181 tcttctaccg gcaaagttga taattctttt gccggaacga ctccttcaac tccagcccct
     3241 tcgattccga caatcacttg tttgtcttca atgactaaaa cttcgccctt aacaacatcg
     3301 ccaactttta cttcttgtac gcttttcatt gcgtcttcca ttgactggtt gttctcttga
     3361 ttttgatcaa attctgtcat aacacttgtc ctcctaacca acattctgcc gtaagtacgg
     3421 tactgttttt agtttaattg attattcaaa aaaaataaag cgatttcgtt cgttttcctt
     3481 gaaaaaacaa ttgtttttca ttttttttct taatttaaaa aagaagataa ccttttttca
     3541 aaacaacagc ttttattgct gcaacgactt ctggaatcga tttgccagtc gtatcgatac
     3601 gaacagcatc ctctgcttgc ttcagcggag agacgtccct tgttgaatca taatggtctc
     3661 tttcctgtat ctctttcgtc agtgtttcaa gatcagtcgg aattcctttt tcttggttct
     3721 ctttgaaacg tctttctgca cgctcactta cgcttgctac taaaaagatt ttcacttctg
     3781 cattcggtaa tacagctgtc ccgatgtctc ttccgtccat gacaacgcca ccgcgttccc
     3841 ctatttttcg ttggacagca actaattctt ctctgacttt tccatgtgca gagacttcag
     3901 aaacagcttt tgtgacatct ggttgacgga tttctaaagt cacatctttt gatgctatga
     3961 atacatgctg tccatcttct tgttgcgaaa aagagatagg atactttttg atcagcgaaa
     4021 ctagcccttc ttcatctgaa aaagatacat tgttcgtaat agctaaataa gttactgcgc
     4081 gatacatggc accggtatct gtataaatat aattcaattc ttttgctaat atcttggcaa
     4141 cggtactttt cccagatgaa gcaggcccgt cgattgcaat actgatttgt gtcatgatat
     4201 aactcctttt tctcagaaaa aaagccgcaa agcgcggcta ttattattta attcttaatt
     4261 cttgaccagg gtaaagcata aagttgtttg gatctaagcc attcaattca aacaattgat
     4321 cagtggtgat ccctgcacgc tcagccactt gttttggacc ttcgcctgct tgaacagtcg
     4381 ttgttgaacc ggctgcagct gatgaagaag gtgttgtttc tgctgcattg ctgcttgaat
     4441 cccctgcttg ataagttgcc tcacttgagg aagtcggtgt tgtctctgct gcaatactgc
     4501 tttctgacgt tacacctgta tcttgagaca cagtagattc ttccgcgatt gtactagatt
     4561 ctactgttga agtctcagtt gactcctctt ttgttgtact tgtagaactt tctactactg
     4621 atgtcgaagt ttcagaagaa gttgctgctg ttgcattgtt gcttgatcca tttctgatcc
     4681 agaaataagc acctgctgga atagcgatac ataagaccag taaaatcaaa aatagcgaaa
     4741 gaaataatgt atttcctttc ttttgttgtc tctgagaact tcttgatgaa acttcatcat
     4801 ctgtatcgta gattggttgt tcccatggtt cttgtgtttc gttattttct gtagattgat
     4861 gtctatcttt tctgctcact gttttacctc ctaaactcta tcgtgtgtat tataccatag
     4921 gaaaaagtgt cttgtcctaa aaaatcaatg ttccatgttt tttttaaata aatttagtaa
     4981 aatcgtttcc caatgaagtt gttcagtctt agaaaaaagc ttcctctttg gcagaagttc
     5041 tgcttttgct ccatcaatat cacaacaatt ctgcggcttt tcggagagtt tttcgccaaa
     5101 ataagctaag atgaattttc tgcggcagtt ttcttcatta atatagcgaa gcatctgctg
     5161 caggcggaat tctttttctt tttcctgacg tttgagccgt tccacccaat tttctggttt
     5221 ttcagactgc tggatcagct ccatccattt tttttgcagc tcatcaaagg gagcttgctt
     5281 tgtggcatat tccaaataaa gctcaaaact ttgtcgctgt tctctagaaa gttgattaaa
     5341 gaaataatgg atacgttcat ccccactttg ataaaaaaga attgctgcac tttcttcctg
     5401 atctctgcct gctcgaccga tttcttgtac atagttttct agactgtctg gcaaatcata
     5461 atggaccaca taacggatat cagatttatc gatccccata ccaaatgcat tcgtagcaat
     5521 gagaaactgc agctgatttt ttacaaactg ctgttgaagt tgtcttcttt gagctgcatc
     5581 aagaccaccg tgatagtatc caactgtaaa acgttccctg aataaatgat acaatcgttc
     5641 gacttctttt tttgttgcac aataaatgat tgccgctcct tgtgctcttt ccatgaagtg
     5701 ttcaagttct tgttcttttt gctgcgtttt ccgaacaaaa agcgagatat tttgccgatt
     5761 gactgattgt ctgatctctt ttggtggtcg atcaaatagt acatgacgta tatctcttgc
     5821 tactaaatct gttgctgtcg ccgttagtgc cagtgtgacc gggtttccta aaagctttcg
     5881 gatttttcct aactgttgat attctggacg gaaatcaact ccccattgag acacgcaatg
     5941 agcctcatct actacatata acgcaatatt ttgttttttc agatgctcta aaagagaagg
     6001 attagtcaac atttcgggac tcaaaaacaa aaatttatat tgcgatagat gacggaggac
     6061 gtatcttctt tcagattgcg aaagtaaact attgaaagca gctactcgtt tctcccctcg
     6121 tttttgtaat tgcgtcactt gatcttccat tagagaaagc agcggtgaca cgatgatgac
     6181 cagaccttcc attaaatacc ccgtcaattg atagcaaagg ctttttcccg ttcctgtagg
     6241 caaaatagcg agagtatctt ctccatcaag caacgcctga ataatttctt cttggccggg
     6301 acgaaatgaa tcaaatccaa aatatttttt taattcattt attaacattc tgtatcgctt
     6361 cctttctcaa tagatagatt tgatacaaac gaaattctcc ataatctaat tgtccatcta
     6421 cattccattc gctaaagcgc cactctcgca cggaatcctt atttggttcc aaacgttcga
     6481 ttgtttctgc agagagaatc cactcgtatg gaaaatcatc aaaaaacaag gcccactcaa
     6541 taagatgatc tgttactgtt cctcttttta aatgacggat tgccatgatc tggtcaattg
     6601 tctgaccatt cataaacatc tgcttggtga tcatcatgct ttgattgaaa ttttgctgta
     6661 ataatgaatc gatcaaagcg tgccaaagcg aatctggccg tttttcgatt tgtgctaaca
     6721 aaaggtggat acatctgctt tgttgcagat aaacctctga ctcatcattg ttttttgcca
     6781 gctgataagg aagcagccct gttcgctcat ttccagataa ttggttagct aaaaaatctg
     6841 ctgcttcttc tggtatctcc gaaaacagct gcaccaaact ctcatacgca gtatcaatca
     6901 aaatttcacg tggcaattgg ctttcactaa gccatttttt tagttggaaa gtataaaaag
     6961 gacttgtctc tgctggaaga tagtcttgaa tacccgttgc cagattagaa attacttgaa
     7021 cagcaaattt aatgagccgc caactagttt cccctgttct gccatagcgg tcaaaacgca
     7081 aaccagtatg ttctatttga tgttcggaca acatatcagc ccctgcaggc gttagctttg
     7141 cttcattttc atttatttcg atccaacctt gctgcgcaat tttttgcaaa atttgataaa
     7201 acttatcctg cttcaattct ggaaaactgt tatggataaa caaaagttcg tgggtaaatc
     7261 cataaataag tactgaagtc gttcgttttc ccacaaggag ttggtaaaga gaactaatcc
     7321 gcagcttgtg tcctgatgaa aatagcgata aaataaaaac aatcatatta ttctccttaa
     7381 gttaggatgg ttagaaatgt cactactatg taaacttgta cctgaacgct gtattgcatg
     7441 cggtctttgt caaatcgaag cgccagatat tttcgattat gatgaagaag gaatcgtttt
     7501 atttgcccaa gaaccagagg ctcatcaaca gttcgtgatg ccggaagaag aaacatatgt
     7561 aaaaaaagcg tatcaaaaat gtccagttcg agcgatttta ttagaaaata aaatataaat
     7621 ataaacgaaa ctttaaaaac gtacactaag ttctctaaac ttgaaaataa gccgggatct
     7681 gtttctcttg ttccgtgaaa tcccgactta ttttgaaaga ttcatttgct atctatagtt
     7741 aattgactag ttttttcacg gaccctatag gacccattta tattatttca actttttcag
     7801 ccagttttcc aatgtattga cgatcacagt ttgctggtct gcgttagaaa tttctgcctc
     7861 tccatctcct ttttgctcac catagctgcc aaaacctcca tgatttccgc cattgattga
     7921 tacatactct gttctttccg gtaaatactg cttagcttct tgataagctt cctgattcaa
     7981 tactttatct tttgtcgccg tgatcgataa aacaggaaca gctgattctg tcaatgttcc
     8041 ttttttgtcg ggataacttg ccatgaagaa tacccctttt aaatgtttgt ctgaatgatt
     8101 cttattgaca taacgacttg ccattacgcc acctaatgaa tggccaccga tcacataatc
     8161 ggaatttgga aaatcttctt tgaccttatc cgctttgttt ccgcccatca ctgctagatc
     8221 aagtggcatt ttgacaatag cgactgaaaa gccgtttttt gctaaatctt gcgcccaaat
     8281 actataactt tctggctcta cgagagcacc aggataaaaa ataagtaatg gagactttcc
     8341 atgattcttt ttctctcctt caaaaaataa gtaatcttct ttgttttttc cttcttttac
     8401 caacacttgc gcttctgacg atggttcgta cgtattttgc tgtatatagt aataaccggc
     8461 tccaccgagt aatcctaaaa tgatgactaa gccaattagt attcgtctaa atattttcac
     8521 tttctcccac cttttctatc tgagtgaccc ctttttctta atcaagtatg attatacctt
     8581 aaaaaaataa gagagaaatg caaactttcc agcatcaaat aaaaaaagac ggaatatcca
     8641 tttgaatatc cgcccttttt gtatgtttat ttttatttag cacataaacg atacacagct
     8701 tctgcataaa tttccattgc tttatacata ctacttaatt cccatcgttc attcgcctgg
     8761 tgcataaagt caggagtatc agggaacatc gcaccgaatg ccacacactg gttcatcgta
     8821 cgagcaaaag tagcgccgcc tgatacaaac ggttcagtca tgtctccagt aatatcgcga
     8881 tatgtcccca atagattttt cactaactca ctgtctagtg gcacgtataa tgaatcaagg
     8941 aagtcaaatt cttcataagt caacccatat ttttctgctg ttttagttaa tttagctact
     9001 aaatcatctt tcttgaatgt aacagggatt ctcatatcta caccgatttt tgtttgttct
     9061 ggtgtgattt ccaaacttgc aaagttcatc gttagctcgc cggattgttc atcttctgtt
     9121 ttgcccacga cagcttcacc agttgcattt tcttgaacta attttcctaa gaaattgatt
     9181 gggccaaaat caaatacttc tgaaagagca attgccagac gagaaatagc attcactcct
     9241 tgtgctgcat ctttcgcgtg gatactttta ccaagaacga cgatgccttc accttgttct
     9301 tcgaaatcga attcgtgttt tttcaatgct tcttttactt cagaaagctt ttctccagag
     9361 taaggagctg catcaggaac aacattcaac gctcctccag cttttacaga aaactcgttc
     9421 gttcctggtc ctgtcaagta agcttgcaat aagccttttt ctgcataaat taaaggaaat
     9481 tcagcatctg gcgcaaatcc ttgcgtaatt ccttcttctt tttcgttata tttttctaaa
     9541 caacgccata aattttcttc atctgtacca aagataaaac gaatacgtgt cttgaattcc
     9601 acgccagcat caatcaaagc tttgacggca tacattgccg caatagaagg acctttgtca
     9661 tcttgggatc cgcgaccaac taaccagccg tcttttatag ttggatcgaa tggttttgtt
     9721 tcccaattat tttcgtcccc tgctggaaca acgtccatat gacacaagat accgaataat
     9781 tcatctccag agccaatctc tgaataaccg taatagcctt ctggatcttt aaatgttcta
     9841 aatcctagct tttcactgat ttccaatact ttatccaaag cttcaatcac ttttttaccg
     9901 aagggatgtc cttgtcctga atctgcttca tctagtacgg aaggaattcg gatcaattcg
     9961 ctcaatgatt ctaatgcttg ttcatgatgt tgttctgtca caaatttttt catgatatta
    10021 tcctcctaat atgttaaaaa gggtcatgtc aaattcctcc gacacaacct cttcttctca
    10081 aataatgacg ttttaagcct gctccatcag caagcagaat gttcttaaca aatgatgaaa
    10141 gaaaaaacac tcactgaatg gagctattga cttgtccacg tgcttatcga tagccgttta
    10201 tcggtttctt cattcttatg atagaccttg tacctgtatt tgtctaggtt tagaacattg
    10261 ctgctacacc taggatcaca cagatggcta caaataatat cgcaattaat ttaccggtaa
    10321 atttccacca cactgaaaca tcgaaacgag caattgctac agcacccatt acgatagcag
    10381 atgttggtgt aattaagttt actaaaccag atgcagattg ataagcagtg attactaaat
    10441 ccttaccgac gccggcaaat tcacccatag gtcccatgat tcccattgtt gcagcagcta
    10501 agccggaagt tgaaggaatc aagaatgaca tagggatata gaaaataaat gtaataatga
    10561 tgaatacact tgaagataat gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg
    10621 tgattaatcc attgttcata accacttgga taccacgagc aacagctact acgattgcta
    10681 caccgatcaa atcagaagca ccagctaaaa attcagagac aaattcagac tctttcatgc
    10741 cataagtgat tgctactacg atggacatta ctaagaataa catagtaatt tctgggaagt
    10801 accagtcacc taatggaagc atgcttttac ctagaaccgt acctagtacc ggaaggtttg
    10861 ttaaccagtt tgtcaatgat tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa
    10921 tcaaactgat gatcatgata ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt
    10981 ttttcgtgat tacttcttga cgaccgattg cttcaatatc gaaatgcttc atatcttctt
    11041 tacgttggtt gtatactaat gattttgttg gatctttttc gatctttgat gcataacgat
    11101 acacatatac aatagaaata gctaacaaaa taacgaacat caagaatcga agaccaatac
    11161 cttcacccat cgaaatccca acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg
    11221 ttgaagctag acagcctact tgagacccta ctaatacgat tgctacagct actactgtat
    11281 caaagcctac agccatcatg actgggatca gtaaagcata gaatggaatc gtttcttctg
    11341 ccattccgta agtagagccg cctaatgcaa ataacagcat caatactgga ataagcattt
    11401 tttcttttcc tttgaatttc ttcacaactg aagcaatccc ggcatctagt gcccctgttt
    11461 tgttgataat acctaaaaat ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag
    11521 caggtgtttc tttagttagc tcccccgctg gtacacctag catcccattg attggcgcca
    11581 tgaaaatatc ccacagccct tgaggatttt gttgtactcg ttcatatgat ccagcgacga
    11641 tgttgcctgc atcgtctaca ctatagtgcc cagctggtat aaaccaagta agtacagcga
    11701 ttactgcaat aataataaat aagaccgtat acgctgaagg catttgaaat tttttctttt
    11761 ttgtttgttc catcttcttc ttcctttctt ttccgttaga gacaacaatt ttggctctaa
    11821 tgttatttta tcaaaaagag tatcagaaca aaaataataa cacgcatttt gtaaaacttt
    11881 tgttcaaaaa gttcgttttt ccctattccc tcctcttttt tcaggttttt aaagctttct
    11941 tctttttcat tgtagcgttt cctttttttt ataaacaaac attttcgtct tttaaaagga
    12001 gaagaatttc acatttgcaa agaattgatt gatttttatg cataaatcaa aaaaatactc
    12061 aaaacaaaca aataaaacgt ttacattatt gcatgtcttg tatttttttc caaaaaaaaa
    12121 gaccaagaaa tttcttgatc tttttgatat ttatttgact gctgtatgac gttgttcgaa
    12181 ctgatgttgt tttcgactta accatggaag aagttgcgca tgcagcacta aaaagacagc
    12241 actcacgatg aatcctttga tcaagttgaa aggaacgatc ccaatcagta catagtttgc
    12301 taaagacatg cccagcatct gtgttgcgtt cagccctaaa aacattaagt aaagaggcgt
    12361 aataacgaaa taatttgcga tactcataaa aatagttaga gcgatggttc ctgtaatgac
    12421 acctaaaaat ttgtttcttc ctttatgctg tttctttaca ctaaagaaat aataaatcgg
    12481 gaatgtaaag atcgttgttg ataagaaact agcagtgtct cctaccaaat tatccggaga
    12541 aaaacctgta gtcagcagat gtaatactga acgcaagaac gctgttgcaa ctccggccgc
    12601 tggaccgaat aagaacatgc tgatcaatac cgagatatca ctaaaatcga tcttcaaaaa
    12661 actaaaagcc ggcatgatcg gaaaagcaaa atactgtaaa actaatgcga tcgcagcaaa
    12721 catcgctact gcaaccattt tctgtacacg ggtgttcttc atttgaaatc ctcctgttag
    12781 gactcctctt cagcgaactt tttgacgttt ctctccaaat gaaaaaactc tattttccag
    12841 ggaaaataga gttagagtta tttgatcaag cgtcgtcaaa taagctctat cttctttatc
    12901 cagactatac tgtcggtatc ggaatttcac cgattcagct atttttttgc aaaataggtc
    12961 gtggactata accaccggtc gggaatttca ccctgcccct gaagacgaac cttatataat
    13021 ttttgtttac atattcatta tacatatcta ttcaaaaaac gcaacatcta acacttataa
    13081 aaagtaagtg ttcattttga gtggttcagc aaactgctga cttctttttt gttcagttca
    13141 cggtaatccc caggacgtag gccttgtaaa gtcagatctc cgtatttttc acgttttagt
    13201 ttttgcacag gaagaccaac tgcttgaagc atatttttca cttgatgatt acgtccttcg
    13261 tggatcgtca attccacgat actgtggttt gtcttaggat ccactgataa aatttggaat
    13321 ttagctggag atgtttttcg tccttcgatc tttattcctt ttgtcaaagg tgcaagcatc
    13381 gattttgtag cgatcccttt tacctttgca acgtaaactt tatccacttc atgttttgga
    13441 tgtgtcaggc gctgagcaaa atctccatca tttgtcagta gcaagatccc cgaagtatcg
    13501 taatccagtc gacctacagg atagatacgt tcttttacca tcggcaaaag atccgtaaca
    13561 acttttcttc ctttgtcatc tgcaacagca gaaataactc ctttaggttt gtacaataaa
    13621 tagtaaccgt attcttcttg ataaattggg acttcatcca cttccacagt gtcatgtttc
    13681 ccaactttta tccctaattc ccttacaact tgtccgttta cttttacacg tcctgcaagg
    13741 atgaattctt ctgcttttcg acgagaagtg atgccagcgt gggcaattac tttctgcaat
    13801 ctctccatta caattattct cctttattca ttccatcaaa gaatagatct ggtgacacgt
    13861 cttcttcaag tgaaatttcc atttcttgtg tatcaggcag ttcttctaat gatttcaaac
    13921 caaaataatc taagaagtag ccagttgttc catataatat cggccgacct ggtccttcta
    13981 ctctaccttt ttcttcaatc agtttatggg cagctagacg ctgtaccgct cctgaaacct
    14041 gaactcctcg aatctgctcg atttcagctc ttgtgattgg ctgtttataa gcgatgattg
    14101 ctaaggtttc gacggcggct tgagataatg tgtttgatgt cccttgcgca tatcgcttaa
    14161 gtaagggagc cagctctttt ttagtcgtta atacataatg gctttccgtt tcaaaaatat
    14221 gcaaagctga gtgcgagttt tgagtgtaag attcttttaa ttctttcact aaacttgtga
    14281 cttctgcttc gctgagattt aacagtccgc ttaattcttc attactgatc ccttcctcgc
    14341 caacaataaa aagaagggct tcaagttcac ttaatttcgt catgttcggt atctcctact
    14401 gctggataaa gcataattga ttcatagttt tctttttgtt gcgcaatgat ctgccctttt
    14461 ttcatcaatt ctagcaaagc cataaaagta gtgacaactt cacttttaga aggtgactca
    14521 aaaaaagaat cgaaagtaca gccatgcgca tttcttttta tacgctcttc gataaatacc
    14581 attcgttctt cgatcgttgt gtcatcgggc gtgatcgttt tttccacttt ttgagaaaat
    14641 ttcttacgtt ctaagatttt ctgcatggca cggatcaaat cgattttttt gacttgtcct
    14701 cgcttcaacg gcttttcagc atccgctaac ccatcgatgt tcataggttc ttttgtgtaa
    14761 taaagacttc gttcctctgc tttttctgtc agtctttcag ctgcatattt atatttacgg
    14821 tattctaaaa gctgagtgac caattcttct cgaggatctt cttcccataa ttcttcatcg
    14881 tctgtgccga attcttggac aggaagcaat gtcttgcttt tgattgccat caatgtagca
    14941 gccatcacta agtattctcc tgcaacagct agctcgagtg ttttcattgt atggatataa
    15001 ttcatatatt gctccgtgat cgtagcgatc ggtatatcat atatatcgat ttccatttgc
    15061 tgaatcagat gaagcagcag atcgagagga ccctcgaata catctaattt aatattgatc
    15121 tccgtcaact cattcactct ttcttctttt tctttgctag ccattttcca atgtaaggct
    15181 ctgtgatttc agttgcacgg atggagacat ccgggtactt ttcctgtaat tcatctaaca
    15241 tttgaaagcc catattctca ccgcgataag acggattcaa agaaatgtca tggatgatca
    15301 gctgatcgtt cgttgcccag taaataccaa taatcccttg aatgttggtt gaaccttctg
    15361 gcgtaaagaa aaaaagttca taatcgtctt cagattcgta cgtatcaatc tccttcaaca
    15421 aagaagtttt acctgtcagt ttcttatgga aactcaatag tcccatagca attttacggt
    15481 tttctttccg gtaatgtgtt aacatcttct caccttccta tgactaagcc cttggaaaat
    15541 gctgcttata aacatccgtc attcttttct tcgtgatatg tgtataaatc tgagtcgtag
    15601 agatatccgc gtgacctaac aattcctgta ctgtccgcaa atcagctcca ttttctagca
    15661 gatgtgtggc aaagctatgc cgcagcgtat gtggtgttac ttctttgtaa atcccagcct
    15721 ctcgtactaa ctgcttcaga tttttccaga ttccttgccg agaaagacct ttcccatgat
    15781 tattgacaaa tacatgcatt tcagatgcgt cctttacaag taatggacgt gcttcgtcta
    15841 agtagcgttc cagccattgg atcgcataat cacctaaagg aatgatccgc tctttatctc
    15901 cttttccaac tgtctgtacc agtccaagag aaagatgaag atctcccaat ttcaatccaa
    15961 tcaattcact gacgcgcatc ccagtagcat acatgacttc caagattgca cgatctctta
    16021 tccctaacgt ttttgtcgta tcaggtgctt caatcagctt ttctacttct gtcagagtca
    16081 atgtacttgg taatttctgt gctttcttcg gcgtatcgat atgttgcatc ggatcatgat
    16141 cagtgatacg ctcttgccgt aaaaactgat ggaatcttct gagacttgag atcatccgag
    16201 taacagttgc tgtcgctttt ttttcttcat gcaacttctc cagaaaacgg atgactaaaa
    16261 agcgatcgac atcctgccat gaagcaattt tctgctcctg tagaaaatgc aagtactgtt
    16321 ccaaatcacg ttcatagctt tgtcgtgtat tggcggacaa tccccgttca atattcaagt
    16381 agtggagata atcgttgatt tgctctttca taataatccc cctcatgcct ctctatcata
    16441 tcagataccg cttcattctg atagtctttt tccataatct gagcaaagaa aaagccgtct
    16501 agacactcag acggactggt ttctttt
//
LOCUS       DACCCS010000062        16358 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.063, whole genome shotgun sequence.
ACCESSION   DACCCS010000062 DACCCS010000000
VERSION     DACCCS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.063"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(143..799)
                     /locus_tag="IVK82_002182"
     CDS             complement(143..799)
                     /locus_tag="IVK82_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6393227.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            992..1270
                     /locus_tag="IVK82_002183"
     CDS             992..1270
                     /locus_tag="IVK82_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393228.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1289..2497
                     /locus_tag="IVK82_002184"
     CDS             1289..2497
                     /locus_tag="IVK82_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6393229.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2645..3010)
                     /locus_tag="IVK82_002185"
     CDS             complement(2645..3010)
                     /locus_tag="IVK82_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP6393230.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3157..3930)
                     /locus_tag="IVK82_002186"
     CDS             complement(3157..3930)
                     /locus_tag="IVK82_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6393231.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4104..4964)
                     /locus_tag="IVK82_002187"
     CDS             complement(4104..4964)
                     /locus_tag="IVK82_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP6393232.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4964..6382)
                     /locus_tag="IVK82_002188"
     CDS             complement(4964..6382)
                     /locus_tag="IVK82_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6393233.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6439..6888)
                     /locus_tag="IVK82_002189"
     CDS             complement(6439..6888)
                     /locus_tag="IVK82_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6393234.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7333..7596)
                     /locus_tag="IVK82_002190"
     CDS             complement(7333..7596)
                     /locus_tag="IVK82_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP6393235.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7695..8990)
                     /locus_tag="IVK82_002191"
     CDS             complement(7695..8990)
                     /locus_tag="IVK82_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP6393236.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8987..10258)
                     /locus_tag="IVK82_002192"
     CDS             complement(8987..10258)
                     /locus_tag="IVK82_002192"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP6393237.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10423..11292)
                     /locus_tag="IVK82_002193"
     CDS             complement(10423..11292)
                     /locus_tag="IVK82_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP6393238.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11584..13194)
                     /locus_tag="IVK82_002194"
     CDS             complement(11584..13194)
                     /locus_tag="IVK82_002194"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP6393239.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14387..15727)
                     /gene="glnA"
                     /locus_tag="IVK82_002195"
     CDS             complement(14387..15727)
                     /gene="glnA"
                     /locus_tag="IVK82_002195"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP6393240.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15755..16147)
                     /locus_tag="IVK82_002196"
     CDS             complement(15755..16147)
                     /locus_tag="IVK82_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6393241.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4666 a         3469 c         2783 g         5440 t
ORIGIN      
        1 ggctcacaac ctcttggtaa acggtgttca atcagctgac cctcggtatt agctcaaaaa
       61 acggcaaaat atgaggaact atttttccgt ttgtttctct aatactcagg tcaaaacgct
      121 gatttcacaa cctctttgtt catcaaaaga taatattgtc ttctaacaag tccatattca
      181 aaataacgat atgatggtct ttaacggtga taataccatc ttttttcagt tgttttaaaa
      241 ttctgtttac actgctagca gttgagatgc cacaaaattt ggcgatttct tcattcgtga
      301 tcgaaaaatc gatcagcatc ccttcttctg tccgcacacc gaataaatca tataattcgt
      361 aaagttgcgt acaaacagct cccagctttc cgtttaatag catttgctgc atttttttca
      421 tcgaataaag cagacgatag cggtaatact cttttacata gtgttgtagc tttgggtctt
      481 ggttgatatt ttgccaaaag cgcacacgat tgattcgata aaactctgca tattctgatt
      541 caactcgaat attatacggc gaatcgatga attgtgaata ttcgtctttt aaaagagaga
      601 cgatttcaag atctttgata tagcgaaggt taaattctct cccatcattt gaaataacac
      661 ttgcttttac cgttccctct ttcaagatat acgtatggga atcttccatc ccttcgaatg
      721 tcaaatattt atgatatcct ttgcgtataa tcggaaaatc atttttatcc aaatattcct
      781 ttaagactct actatccaat gatgctcccc catccaaact ttcatctaaa aagatcatac
      841 agtactttaa aactttttaa agtccaaagg tattttacta atgatattaa catttgttaa
      901 gtaccttttt ccatcactta tgcgattttt ttcctttttt ttattcgtta tactgcagaa
      961 gtaaaatttt ttaagaatag agggaaaaaa catgaattac tggatgaaca caatcattaa
     1021 tcgattagaa acagcatatc aaacaagatt tgatatgaaa gcttctttag tctttttaaa
     1081 tgatgcgtat caaaactcga tcgaattgat caaagcagtg gatgagaatc caacaaacga
     1141 atgcgaagaa tttttaaatc tgtttatgtc tacaagagat ttatttattc gccaacttgt
     1201 tgatcgctat ccatccaatt atcacgatgt agaagttcaa atacaaaaat taaaagcata
     1261 ttcagcttaa ggggaatgta taacatacat ggaaaagaaa ttatttaata aatattttat
     1321 tggaatcaca ttgatcaatt ttattgtcta cctgatttat tatctgttga tggtcatcat
     1381 ggcagtcatt gcgcaagaca ccttacacgc cagcttgagc caagcaggtt ttgcttctgg
     1441 gatctatatc atcggaacat tgctggcacg cttattcatg gggaaacagt tagaactatt
     1501 tggtcggaag tttaccttaa gaggcggcgc gatcttctat ctcctttcaa cattagcata
     1561 tctttatacg ccaactatcg gggcattgta tttagttcga ttcttgaatg gattcggcta
     1621 cggtacagtt tctacagcta caaatgctat cgttactgca tatatccctg catctaaaaa
     1681 aggggaaggc atcaattatt atggattaag tactagttta gctgccgctg ttgggccatt
     1741 tttaggaatg attttgctga atatgactga ttttcgtttc attatctggt tttcaattgt
     1801 actggtcttc tttgttacga ttgcctgcct tgtctttcct gtaaaaaata ttccattatc
     1861 tgaagaacaa aaaatatctc ttcgctcatg gaatatcaat agttttattg aaaagaaagc
     1921 acttttcatt acaggaattg cctttttgat gggtctttct tattcaagtg ttttatcatt
     1981 tttatcttct tatacaaaag taatccactt agttgctgct gggtcatttt tcttcgtcgt
     2041 atatgctttg gtcatcacat ttacacgtcc attgactgga aggattttcg atgtcaaagg
     2101 agaacaatac gtgatgtatc ctagctattt attcttaaca gcaggattat ttttactgag
     2161 tgtaacgacg aatagtgtga cattgcttat ctctggagca ttagtcggcc taggttatgg
     2221 tacgtttatg tcaaatggac aggctgtttg tttaaaaatc gtccgcgaac atcgaatcag
     2281 tattgcttta tctacctact ttattggttt agacctaggc ttaggcgttg gtccttattt
     2341 gatggggatg ctaaaatctc ttacttcatt tagaggattg tatgtgattg ccggagtcat
     2401 tccattgata tgtacagcac tctatcttgt taatggccga aaacatagtc atgaagcaga
     2461 gggcgaattg attgaatcat tgaaaaatga aatttgatct tgatgaatag ataaaaaaaa
     2521 ccgttcagat ctgaaaataa tctgagcggt tttttgagta aaaatagttt atgattattc
     2581 ttaattaaac atctatttat ttttaaagct ttcatttatc gataagaata gtttaactgt
     2641 tgatttattc cattttccgt tcatctggta cgatgccttg acttagcaac cacttgtgga
     2701 agaaccattc tgccacaccg atcaatagag caggaatcaa tactttcatc caaatatcga
     2761 ctgtatcatt cataaaatat agatagagcc acaagataac tgcagctgaa ccagcatctg
     2821 cgatcgtcgc tgcgatgttc cctactttac gtaagatcaa cagatcccct agcacataga
     2881 tcatgactac aataattacc ccaacaatcg ttgaatccat aaagctaaca ccgtataaac
     2941 ctgtcagcac gacccacaga atcgcccaga tcatgattgc cttcactacc aacgccttca
     3001 catgtttcat tttctgcact cctttctcct ggataaaaaa gtaagattat ctaacaagat
     3061 aaccttttca ttattatacc atcggctgag agaagcgagc aaagataagg tcctatctaa
     3121 aaatgaggaa gccacttaga acattcacgt aataatttat ccgtacagac attcgatatg
     3181 gtgttccttt actttcgcat actgttcttt tgtcagttct ttacttgtca ccagataatc
     3241 gatttcatga aaagcaatgg cttgatgcat gaatttatgt gcaatttttt gctgtgtaac
     3301 taaaacggca agccggtctg attgttttct aaaagctttt ttgaccattg cctctccttc
     3361 atgagtttct gttactcctt ggctgaaatc tagcccgcga caactcatta tcgctaaatc
     3421 cgcataatat gacaatgcat cgttataggc ttttgaaccg ttgattgttg ctcgtttcgg
     3481 aacaattgcc cctcccaata cagatacttt tgccgttgta tattcactca acatagcagc
     3541 tgtgacgatg ccattcgtaa tgatctgcaa accatcaaat cttgctaggt ttctgactaa
     3601 gtgtaaagag gttgtactgg aatccaagaa taagaccata tcatctcgaa tcaaagaaga
     3661 agctgtatct gcaatcttac gtttttctgt ttcttcttct aatttggtga tcttcatcag
     3721 gtaaggcacg tcaatttttc gattgtcaat aagcatggcg ccaccgcgaa ctctacggat
     3781 caatccactt ttttccagaa aaacaaggtc acgtttaatc gtggcttcgc tataatttac
     3841 taactcgatc aaatcggtga ctcttgcaaa ttggttgttt tttagataat tcacaatctc
     3901 ctgctgtcta ttctccactt cattcaacat aaaatcaccc catttacttt atatagtaac
     3961 aggaaaaaag aggttgtgac agaagtgatt ggcttcgaga aataagaaga aatttttcga
     4021 aaaaactact tctgaacatc gtgtgtggtt aagagggtat gacaagacta ttgtcatacc
     4081 ctcttgacca cacattttta tttttagtat aaactagcct tgcctactgt gttcaaaatt
     4141 tccattttat gtttgaccac ttcttgtacc tttttattag ctgctggata aattgcattc
     4201 ggctcgtaca tcgatggatt ttcactcaat atttctctca aagcattgaa aaagacgctt
     4261 ttaagatctg agctcaaatt cactttggca actccgtatt ttactgcttc gctgacttct
     4321 gcgtccggat tacctgaacc gccatgtaaa acaaatggaa gatcgatttg ttggttcaat
     4381 tctttcagta agtcgatatt taattttgga gttctgtttt tcggatacaa gccatgcgct
     4441 gttccaattg ctacagcaag tgtatcgatt cctgtttctt tcacaaaaat cttcgcttga
     4501 tcaggatctg tataaatgat tgtttcagca cctccttcag cagaaccatt attcccaatc
     4561 gttccaagct cagcctccac tgagacattt accgcatgag ctaacgcaac cacttttttc
     4621 gtaatctgta cattgtcgtc gaatgatttc aaagacgcat cgatcatcac ggaagaatag
     4681 ccgtttcgaa tcgcccgtaa gacatcttct atcgtaccgc catgatccaa gtggataacg
     4741 atcggcagtt tacttgtata agcataggct cttactgccg caacaaactc atcacttaaa
     4801 taggcgattt catcaggatg aatttctaag attactggtg cttctaatct ctctgcttct
     4861 tcgatgattg ctttcatcat atcatagctg caaatattga aggctggtac tgcaaattga
     4921 ttctctttag ccactttcaa taactctttc attgtatata acattacgct tccacctcat
     4981 ttgttagatt ttcatttttc gtccacttgt cttgtttttt ctctttcact gtttttttca
     5041 atagtccaag cataaaggct gtagtgactg tacctgatgc aattgccaag acatacatag
     5101 gtaaattgtt cataacattt ggaattagca tcacccaaat ccctccatga ggtgccattg
     5161 aagtaactcc tactgccatg gaaatccctg cagcgactgc tgaacctgtc atcaaactag
     5221 gtatcacgcg gatcgggtca gataccgcaa acggtatagc accttctgta atatacgaag
     5281 cgcctaaaat ccagcatgat tgacctgaag tccgttcttc tttggtaaat ttatgtttga
     5341 ataataaagt ggctaaagca atacctaggg gaggtgtcat tcctgcagcc atacaagcag
     5401 caatcggtgc atttactcca gctgacatca aaccgatcga gaaagtcgaa gtcgatttat
     5461 tgatcggccc acccatatca gctgccatca tgatcccgat aagtaatccg aacaatagcc
     5521 cggatgtatt tcccaagcta ttcagccaat ttgtcaaagc atccaacacc catgtgatcg
     5581 gttgttcaat cacaaatacc atcaaaagcc caataattaa gatcgataaa aaggggacga
     5641 caatcagctg ataaatagca gaaaattctt tttttacgct aattttattt gccagaaaat
     5701 cacagatata ccctgctagc acgccaccaa tcattccgcc aagaaaacct gaaccacctg
     5761 tagaggccag taatccggca acaattcctg gggcaaaacc tgcttttcca gcaatcgatg
     5821 tagcaatccc cgcgcctaac gcaggaacca tcatggcaaa cgctgtatct cctccgatcc
     5881 ttgaaaatgc agctgctaac acattatagt ctgctgagtt gggatcagcc gctgtaatcc
     5941 caaaggcaaa actcagtgcg atgatgattc cgcctgcgat cacaaatgga agcatgtaat
     6001 tcacaccatt catgaaatga ttatagattg tcggcttttt ctcatttgca gctggttctt
     6061 tttcattaga cacaacgaat acgccaatat ctctcaatgc ttcgtctatt actacttgcg
     6121 gttctttgat tgctcggctt gtagagacac gctttacttt tttcccggca aatcggtcca
     6181 attcgatttc tttatctact gcaagaatga ttgcatctgc ctctttgatt tcttgttcag
     6241 ataactgatt ttctgttttt acaccttgtg tttcgaattt tgcttgatgg tttagatttc
     6301 ttgctgttac ttctaatttt tctgccgaca tataagtatg agcaattcca gtcgggcaag
     6361 cagttacacc aacgattttc atgattattt tcctcctttt tcttcactat cgatactcgg
     6421 acaaagactc tactattttc attcttgact aatctgtctg atgatatctg ctggcgtagt
     6481 ggcttctttt attccctttc taaaatctgc attcatcaat cgtctggaga gtgtgctgag
     6541 aatctctaga tgcatatctg ctgatttttc aggaactacg atggcaaaaa tatattcaat
     6601 tggcatttca tctatcccgc tatagtccgt taatttggct gtatgaaaaa aacacagtgc
     6661 tggttttgtc acatatttgc tttttgcatg gggaatccca aatccatctt ctattcctgt
     6721 ggagatctcc tcctctcgtt tgaatagtgc cttaacgact ttttgaacat ttcttacctt
     6781 atttgttttt ttcaacacat cccctattcg ctcaaataat gatccttttg aatctatcga
     6841 tggatcgaaa acaatcagtt cttctgcgat caattgatca aacctcataa aaaacctctc
     6901 ccccacaaaa aatgttaacg ttttcttgga actgaggtca gtatagcatc gctagctgta
     6961 ccgttcaata ttatgaatga acagaggaaa aagacccatc atccgcgtgt ataagtcatt
     7021 aaaatgaacg atctagctca caaacaatcg caagtctata acaaaacaaa aagccttcct
     7081 tgaaaggaaa gcttagcttc taattttagg taaatggttt tttacttcga aataacccct
     7141 cttcatttcg ttaatataag gtgtttttat caagtggata attaaaacta tatactagtt
     7201 tttcattgtt ttgtgcatta tgatcctacg actttttcag catcaaacga agttttaaat
     7261 tggcaaaaaa cataaaaaaa cagactatcc ctatcagata gtctgctaaa cgttgagtta
     7321 tcagcgtttt tattattccg cagcgtttgc gtctttgaga ccgtattttt tgttgaaacg
     7381 gtccacacgt ccgtctgctt gtgtgaattt ttgacgtcca gtgtagaatg gatgtgagtc
     7441 agaagttact tcgacacgga tgactgggta tgtattaccg tcttcccatt caactgtttc
     7501 ttgtgaagtt tttgttgaac ctgacaagaa tttgaaacca gttgttgaat ccatgaatac
     7561 tactgggtgg taatctggat ggatgttttc tttcatgatt ttttcgctcc tttgccctgc
     7621 tccatttgct gaaccagagt tgatttgtcg atttacaaca ttaacatctt aacatgtttc
     7681 agtcaaggtt gcaattatct tttcagattt ctggtcggat tttttttacc aaaggagaca
     7741 tcttgaaatt ctttgaaaaa ttgctgattg ttttttgttt tacgcaaaaa gcggatcagt
     7801 tgttccgtat attctaatga atctccagtc atattttttc gaagtttcca aatttcttct
     7861 aattcttcac ttggcatcaa taattcttct tttcgggtac ctgatttttt gatatcgatg
     7921 gctgggaaga tccgacgttc agataattca cgtgaaagtt gtaattctag atttccagtt
     7981 cctttgaatt cttcatagat cacgtcatcc atacggcttc ctgtatcgac taaagcggtc
     8041 gctaaaatcg tcaaactgcc accttcttcg atgtttcttg ctgccccgaa aaatttctta
     8101 ggtttataca atgcggctgg gtcaatcccg ccactcaatg tacgtccgct tggtggaacg
     8161 actaagttat aggcccgagc aagtctagta atactatcca tcaagataac gacatctcgt
     8221 ttatcttcga ctaaacgcat tgcgcgttcc aatactaatt cagaaactcg agtatggttt
     8281 tgcggctgca gatcaaatgt agaagatacc acgtcgcctt tcacgcttcg ttctagatcc
     8341 gttacttctt ccggtcgttc atcaatcagt agaacgatta attctacttc cggatagttt
     8401 tcggtgatac catttgcgat ttcttttaat acagaagttt tccctgcttt tggcggtgcg
     8461 acgatcaatc cacgctgacc aaaaccgatt ggagaaaaga tatcgatcat tcgggtagat
     8521 agtctttgcg gtgttgtttc cagttgaagc tgtctttgcg gataaagagg tgttaaggct
     8581 ggaaaatgcg gacgttcttt tgcttcttcc ggatcttttc cattaacgct ttccacgtgc
     8641 atcaaaccat aataacgttc cgattcttta ggtggacggg cttttcctgc cactttgtcc
     8701 ccatttctca aactgaatcg tctgatttgt gaggcagaaa tatagatatc ttctgcactt
     8761 ggtccataat tgattggccg taaaaaaccg taaccatctt gaccaacgat atccaatatt
     8821 ccttccatat aataaaatcc ttgtttctct gcttgtgcac gaatcacagc tagagataat
     8881 tcctttttat tcatcttact gtaataagga attttaaatt ctttggcata actataaact
     8941 tcttttaacg ttttgttctc tagttctgcc attgtcaaat aatcgctcat tcagctccca
     9001 cttcctctat catttcaatt gatgccccta aggctgttag tttctcgata atatgatcat
     9061 aaccgcgtaa gatataatct acattgtaaa tcgtcgttgt cccttctgcc aatagtccag
     9121 caatcactaa acaagcacct gcccgaagat cagaagccac gacttctgtg ccgtgaagtt
     9181 tatttggacc attcacgatg atcatatttc cttccacggt aatatcagcg cccattcgtg
     9241 ctagttccgg cacatggttc acacgtttcg catagatcgt atcgatgatt tctgaagtgc
     9301 ctgtagtcat caatagtaac gcagtaagtg gctgctgcag atctgtggca aatcctggat
     9361 aaggataggt catgatgttt gcgggtttta attctttcga ggggtaaact tcgatttcat
     9421 cttcgctgat tttcatatgg acaccaattt cctgcaattt tgctataaag ctttccaaat
     9481 gttcaaagat cacatttttc actttgatac catttcctac tgcagctgct aatgctagat
     9541 aggttcctgc ttcgatgcgg tcaggaatca tgaaatgccg acagccatgt aatgtttcga
     9601 ctccttcgat acggatcaca tctgtacccg ctcctcgaac ttttgccccc atattattca
     9661 gtaaagttgc cacatctatg atttccggtt ctcgtgcagc gttttcaatt actgtctgtc
     9721 cttttgcttt tacagcagct aacatcacat tgatcgttgc tccgactgaa accacgtcca
     9781 tgaaaatccg attgccacgc aatcctttat tttctgtccg taaatacatg gcaccatgtt
     9841 catttgtcac ttcagcacct agagcttcaa atcctttgac gtgtaggtca atcggtctag
     9901 ggcctaaata acagccacct ggcaggccta ctaccgcttc gccaaattta ccaagcagtg
     9961 ttcccataaa ataataagaa gcacgcaaac tattgatttt tccgtgcggc atcggaatcg
    10021 atacgacttc tcgaggatcg atttctagta tgttgttgga aaaatggaca gttgcgccca
    10081 tgatctctaa aatttcaatt aatgaatgaa catcttgtat gtccggcaca ccttccaaga
    10141 tcactgggga atcagctaag attgcagctg gaatcaatgc gactgcacta ttttttgcac
    10201 cgctgatcgt tacttcacca gctaaaggtc ggtttccttt gattacgatt tttttcatct
    10261 gtttccacct tgtttattaa tcttttttca tggatatgct tgtattttat catagataaa
    10321 aaaaagaaac gagcatggtc tgccgtttta agtaaaaaag aatggagagc gacttcaaaa
    10381 gtcgttctcc attcctatat atcgtatctg ttatgtggat gcttacgctt tgttagctga
    10441 accgaaccag tcgatatgag atttagcatc ttcaacgatt gctgctttac ctggagctaa
    10501 taatttacga gggtcgaatc ctttaccttc tttatcttta ccagcttcga tatatttacg
    10561 agtagctgca gcaaatgata attggaattc agtgttaaca tttacttttg agatacccat
    10621 tgagatagct ttttcgattt gatcttgagg aatacctgat ccgccatgta atactaaagg
    10681 catgtctgga cctacagcat cagcaattgc ttgtaagtgg tcaaaagcta atcctttcca
    10741 gttttctgga tattgaccat ggatgttacc gataccgcaa gctaagtagt cgatacctgt
    10801 agcaaccatt tgtttgcatt cttcaatatc agctaattcg ccgttaccga tgattccgtc
    10861 ttcttctccg ccgattgaac caacttcaca ttcaactgag ataccttttg catgtgcttt
    10921 ttcaacaacg tctttagcta atttcaagtt ttcttcaaat ggtagatgtg aaccatcgaa
    10981 cattactgaa gtatagccta attcgataca ttccaaagca gcttcgtaat caccatggtc
    11041 taagtggata gcaactggaa cagtgatgtt cattgcttca actaaatcag tgattaaatc
    11101 tttacagatt ttgtaaccac ccatgtattt agctgcaccc attgatgttt ggataagtac
    11161 tggtgcttgt tttgcttcag ccgcttcaag gattgcttga gtccattcca agttgtttgt
    11221 gttgaatccg ccgacagcgt aaccgccttt acgagcagct tttaaaaatt cagctcctga
    11281 tactactggc ataataaaat tcctcctaag ttttgtaaaa ctaaatttgt taagacaatt
    11341 cttaaccaac gaccctattt taccaaacat ttcggtagtt ttctactaaa atcaatgaaa
    11401 atgaaaattt tcaagaagtt ttttgcaaaa aataaaatat ccaatagcgc tttaatcgaa
    11461 aaaagtcttt tgctttctat cttttttcct acaaataata acaaatcaga aaaagagaaa
    11521 agtgacaaga aaattgctta tccccttctc ttgctacttt tctctttcta tcaagttcaa
    11581 aaattatttt gtttcgtgat ctgctaatga tgctccgaca aatgctttga ttaatttttg
    11641 cgggcgattt ggacgagaaa tcaattctgg gtggaattgg catcccacaa agaatttttt
    11701 ctctggcagc tctacgattt ctactaaacg gttatctggt gagacacctg aaaagactag
    11761 cccttgttct tcaaacaatt gacgatattt gttattgaac tcatagcggt gacggtgacg
    11821 ttcttgcaca acatcggctc catcatatgc ttcagccgtc ttcgttcctt ttttgatttt
    11881 acatgggtat aatccaaggc gtaaagttcc acccaaattc tcgatgtttt cctgatcagc
    11941 cattaaatca ataatattgt ttgctgtatc tggattcgtt tcagctgatg acgcatcttc
    12001 taatccaacg acgttacgtg caaattcgac acaagccatc tgcataccaa gacaaatacc
    12061 taaaaacgga acgtcatttt cgcgagcata acgaatcgct tcgattttcc cttctactcc
    12121 gcgatcacca aatccaccag gaaccaagat tccatctgcg tctttcaaac gttcttctac
    12181 attttctctc gttaattctt gtgctttgat ccaatcgatc tcgatgtctg aattataggc
    12241 gaaaccagaa tgtttcaatg cttcaactac tgagatatat gcatctggta actcgacata
    12301 tttaccgaca agcgcaatcc gtactttttt cttcaagttc aagacacgtt cttctaattc
    12361 tttccattct gtcatgtctg cttctggtgt atctagtttt aaatggtcgc aaacaatttg
    12421 atccattcct tgtgcttgta aaactaatgg gatggagtaa agtgtatcta catctggcga
    12481 ctcgataact gcttctggtt cgacatcaca aaattgtgcc aatttgtttt tcacgctttg
    12541 agaaacaggt tgctctgtac gtaccaccaa aatattcggt tggataccta aaccacgtag
    12601 ctcttttaca ctgtgctgcg ttggtttagt tttcatttct ccagcagctt tcaagtaagg
    12661 aatcaatgtt gtatggatat acataacatt atctgtaccg acatctgctt tcatttgacg
    12721 aagtgcttca aggaatggca aagattctat atcccctact gttcccccta cttctgtaat
    12781 gatcacatct gaatcagtca ttttagctgc tcgcatgatt ttttctttga tctcatttgt
    12841 aatatgagga ataacttgaa cagttgctcc caagtattcg cccttacgtt ctttacgcaa
    12901 aacttcagaa tagatttttc ctgtcgtcac gttggagtat ttgttcaaat tgatgtcaat
    12961 aaaacgttcg tagtggccca gatccaaatc tgtttcagca ccatcatctg ttacgaatac
    13021 ttctccgtgc tgatatgggc tcatcgtccc cggatccacg ttgatgtatg gatcaaattt
    13081 ttggatagtt acttttaagc cgcggttttt caataagcgt cctaaagaag ctgcaacaat
    13141 cccttttccg attgatgaaa cgacaccgcc ggtcacaaaa atatattttg tcataagttc
    13201 aaaaactcct ttttaattta tgtgcaggaa aagtctcaga caataaacaa agctccctat
    13261 tcttcaataa gaatagggag ccgaaatttc tgtaaacttc tgaccctcta aaagggtgcc
    13321 caaggtgtat aatacaagct gactaaggga aagtcaagta ctcaaatgaa tactatccgc
    13381 ttacaatttt ttctttttcg atatcctttg agaaattatt tcttaagtcc tttatgagtg
    13441 caaaaaaaat ttaacccaca taataagctt gatataaaca tagctaacgt taaacctaag
    13501 caaatcagaa aatcgttact gaaaaatttt aagtgaaata atagataaaa aaataaaaca
    13561 aacattatga tagataatat cccacctaac aaagaactac tttttcctac ttgcagcaac
    13621 agtttatata gcttatcgta gaaacaatct ttttctttca cccttattta cctcctttac
    13681 gtaaaaaacg tagtccacag ttctcggatt tttggcatat cttttctatt aaataatatc
    13741 aaaaacaaaa caattccact aattttttca aagatgacct ttttttcaac aaaagtgcct
    13801 tgtagggatt cattgaaggt aacgaatcac ataagtaaaa aataaggaga tgcgacaaaa
    13861 gtcgtgcctc cttatttcta aataaacggt gttctcctgc caactcctcg gtcacaagtc
    13921 acaatatcca tcaaacatga gaagctgttt ttgaaattat ttcttgtgat tcggagttaa
    13981 atggtaggct cacaacctct ggataaacgg tattcaaaag ccagtttctc ggtattaagc
    14041 caaatttatg caaaaatgta gaaagccatt ttcacaaaat ttttcttaat actcgaaact
    14101 aaacgtcttt ttcaaaacct cttggtaaac ggtgttcaaa agcttgcatc tgcggtaata
    14161 agttcagcca attcaaaaac aagaaaagct gtttttgctt ggctattctt attacttgtt
    14221 gcagggcagc tttttcacaa cctctccact aaacggtgtt caatcagctg accctcggta
    14281 ttagctcaaa aaacggcaaa atatgaggaa ctatttttcc gttagtttct ctaatactca
    14341 ggtcaaaacg ctgatttcac agcctctaat tcacggtatt cctgttttaa taaagttcta
    14401 gatattgttc tctttcccac tcggaaacag tttgtctgaa tgccgcccat tcgagacgtt
    14461 ttgcttcaac aaaatttgag aagatatgtt gtcctaatgc gtcaatcatt acattatcct
    14521 tacgcaattc tttgatagca ttatggatag ttgaaggtaa gtcctggata tgtgcagctt
    14581 ctctttcttc ctcatccatt acataaatgt tacggtcaac agctgctggc ggtgtgatct
    14641 gattacggat accgtctagt cctgcttgca acaatactgc cattgccaag tatgggtttg
    14701 cagatggatc aactgaccgc aattccaaac gtgtggataa accgcgagat tctggtacac
    14761 gtactaatgg cgaacgatta cggccactcc atgcgacata aacaggcgct tcataacctg
    14821 gaacgagacg tttataagaa ttgactgttg gattgcaaac ggctgtgtag gcacgagcat
    14881 gtttcagcaa accacctaag aaatgataag ctgtttcact tagttccaat tcaccgtctt
    14941 tatcatagaa aacatttcct tcatcattga acaaagacat attgcaatgc ataccagaac
    15001 cattgatccc aaataacggt ttcggcatga aagtcgcgtg aagtccatgc ttgcgtgcaa
    15061 tcgttttgac aacgagcttg aaggtttgga tattatcaca agcatctacc acatctgcat
    15121 atttaaagtc aatttcatgc tgtcctggcg ctacttcatg atgtgaagct tccacttcaa
    15181 agcctagact ttctaattcc aacacgatgt cacggcggca attttcacct aaatctgttg
    15241 gtgcaaaatc aaaataacca cctttatcat tcaaggtcgt tgtgatccca ccgtcttcgt
    15301 ctaatttaaa taagaagaat tctggttcag gtcctaaatt gaaagaggta aaaccaaggt
    15361 cgcgcatatc tttcaatgct cgtttcaaat tcccgcgagg atcgccggca aatggtgtcc
    15421 catccggatt atagatatca caaattaaac gagcaacttt tccatgagca ctttcccacg
    15481 ggaagatcat ccaagtggag agatctggat acaaatacat atcgctttct tcaattcgaa
    15541 caaatccttc aatcgaagaa ccgtcaaaca tcattttatt ttctaatact ttgtcgatct
    15601 ggctcactgg aacttctaca tttttgatcg tacctaagat gtctgtgaac atcaatcgta
    15661 aaaatcgtac gttttcttcc tcaatgattc gtttgatttc ttcacctgtt atctgtttct
    15721 ttaccatggt atccctcttc cttttttaat ttattcagtc aaagaattgt ctttgcaatc
    15781 cttgcgactg ataatgacct gttttgctta acccaccttg cgccaaaatc tcatcacgaa
    15841 aaatttgtcg aacgtcatga tctgtcaacg gcttcttgtt ttttgcttct tgttttttcg
    15901 cctgtgccat ttcgtataca cgtttgatcc cagccatatt caatccctct gaaagatagt
    15961 ctttaatctc aagcaacata tcaatatcat tcagcgaata catacgtcgg tttccttcgc
    16021 ttcgttccgg atgaactaaa tcttgttctt cataatagcg aatttgccga gcagacaaat
    16081 cagtcagctt cataactgta ccaattggaa atacggacat agatcggcgc aattcctttt
    16141 ctcccatcag acgtcccctt tcgttttata agttggctaa agtgtaccaa ttaaaaaaaa
    16201 tattgtcaac tgcatatgtt aggttttcta acatgagatc tctatttttt ataaaataac
    16261 aaaaaaataa gaacacaaaa aaggtatcta gttgattttc gtaccagata ccttttattt
    16321 tttattatac tgtactatcc tttattagtg tttttatt
//
LOCUS       DACCCS010000063        16198 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.064, whole genome shotgun sequence.
ACCESSION   DACCCS010000063 DACCCS010000000
VERSION     DACCCS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.064"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(854..1384)
                     /locus_tag="IVK82_002197"
     CDS             complement(854..1384)
                     /locus_tag="IVK82_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP6393242.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1381..1998)
                     /locus_tag="IVK82_002198"
     CDS             complement(1381..1998)
                     /locus_tag="IVK82_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6393243.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2580..2801)
                     /locus_tag="IVK82_002199"
     CDS             complement(2580..2801)
                     /locus_tag="IVK82_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP6393244.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            complement(2979..4511)
                     /locus_tag="IVK82_002200"
     CDS             complement(2979..4511)
                     /locus_tag="IVK82_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6393245.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4647..5732)
                     /locus_tag="IVK82_002201"
     CDS             complement(4647..5732)
                     /locus_tag="IVK82_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6393246.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5747..6466)
                     /locus_tag="IVK82_002202"
     CDS             complement(5747..6466)
                     /locus_tag="IVK82_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP6393247.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6463..7773)
                     /locus_tag="IVK82_002203"
     CDS             complement(6463..7773)
                     /locus_tag="IVK82_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP6393248.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(7800..8390)
                     /locus_tag="IVK82_002204"
     CDS             complement(7800..8390)
                     /locus_tag="IVK82_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAP6393249.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8708..9769)
                     /locus_tag="IVK82_002205"
     CDS             complement(8708..9769)
                     /locus_tag="IVK82_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6393250.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10337..11404)
                     /locus_tag="IVK82_002206"
     CDS             complement(10337..11404)
                     /locus_tag="IVK82_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6393251.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11424..11903)
                     /locus_tag="IVK82_002207"
     CDS             complement(11424..11903)
                     /locus_tag="IVK82_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAP6393252.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(11920..12381)
                     /locus_tag="IVK82_002208"
     CDS             complement(11920..12381)
                     /locus_tag="IVK82_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6393253.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(12395..13645)
                     /locus_tag="IVK82_002209"
     CDS             complement(12395..13645)
                     /locus_tag="IVK82_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP6393254.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14023..15474)
                     /locus_tag="IVK82_002210"
     CDS             complement(14023..15474)
                     /locus_tag="IVK82_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP6393255.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(15795..>16198)
                     /locus_tag="IVK82_002211"
     CDS             complement(15795..>16198)
                     /locus_tag="IVK82_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP6393256.1"
                     /translation="LFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCLAQLTA
                     PSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFGKEKVV
                     QMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
BASE COUNT         5379 a         3205 c         1868 g         5746 t
ORIGIN      
        1 tttaaaatta acattgattc tcctatgaaa atatctttta cctttctcct gtgaaaagga
       61 aatttccttt tttatagtgt tattgatata atcagaatat tcatcaaact ctgtatctgg
      121 agttaaaaat ctacagtaac atctataagc tttgctagta atcttcttgg gttctctctc
      181 cattgtcaag caacatcaaa acacctactt actattcgat tcttttctgc caaaaacgag
      241 tcagcatcac tcacaactta tgtgttgctg actccgacta gtcctttgtt caatatcaac
      301 ttattaaaca gatataatat ttttctttat ttcgtatact ccaagacttc catctttctt
      361 tcatcttatc tctgaaaaaa acacagcaaa gtgcccttaa taaatataca ttagaatttg
      421 caaatttttt catcttaaga tatatcctat tcatattctt tacaatccta atgggtgatt
      481 acaaatctat ttagttcaag ttctacatta taaacacctt tcttgatagt atcatctgct
      541 gcacaagcga tgtcgatcca cttattaggg gatttacgtg ctcaaaagtg gctattcctt
      601 ttgggaagtc catccataat aatggtccat ggattttttt agggtcttgt cgttcaaatt
      661 tctcaaaaac tttattatac ttccatagtt ttataattat ttggtttcac tttggctcgt
      721 tcaaacacca acttgtattt cttttcaacg ttcgaactgg agccccccct ataatctcat
      781 tattattttc atgacttttt actaccgtgg aattacaagc aacaacaata ttatctgcta
      841 aatgaacacc tttctatatc acgctttttg ctccaatcca acaatgttta cctataacta
      901 ttttattatt tttattcaaa atcctatgag tattcaattc aaaaatcgta tgtgaatcgg
      961 tatccataaa taatgtatcc cacgatactg tacagccatc tttgaattga atttccttaa
     1021 agcaaattat actcattttt cctgtcgaat taaactttga cccaatatct aaaactgcat
     1081 ttttccttat gtcaatatga gccccttgtg cgataaacgc tggcccttgt attcttagta
     1141 ttccttcaat acttataatc gtttcttcat ttcccgcagt atttaatact ggaaagccca
     1201 aataaactat ttttccagat tgtaatacta tctcaatttt ccctctcgat ttgcgtgtaa
     1261 tccgtacttt acgatgcacc aaaataggta atctccacgc atctgaaata tgaagaaatt
     1321 tataattgaa gtatatcgtt ttaggtaaat ttaatatatt ctttattatc aatccgagtc
     1381 tcatctttca ccatctcttt ttgtaatcaa aattagttta taaaggatta cacccaatat
     1441 accgcccaac gtattatata caaaatccga aatttgaaac gttcctagcc tcaaaaaaag
     1501 ttgtaaaaat tcaattattg ctgaaaaata aaatgcaatt tttcctccat atactattga
     1561 ggaccatatt ttgggtttct ttatattttc atatttaata gacattacca tataaacata
     1621 tggaataaaa agaataatat tttctaaaca cgctgtagat aactcttttg ttccagttgt
     1681 tgtattatac ttataaatcc accagtctcc cataacatca gataaaggat ttaaccaaat
     1741 tgttcgattg attaatgttg taaataaaat catcgcagta tagaatatca aagcacaaac
     1801 ttttctatat tttctatcct tctttaaatg aagataaagt tccattagta tacgtttata
     1861 gccctcacct gcagcaatgc catctgttgc atacataaaa tagaccagtc ctaaaattgt
     1921 caataaaaca gcaaacccaa agggttcata taatttatat aatatatctg taataatttc
     1981 tgcaatcact attatcataa ttatttctcc aatatcaagt atacacttat cgcttccaat
     2041 tacacatgat catattagag tgtattatca cattctctaa tattcaattt atttttccta
     2101 aaatattcga aataatcgtc ttgataacga gtaagtattt ttatacgatt gataacatag
     2161 taacaatatt tttaattata gtaaaaaatc atctgccttt ttcatccacc taatcttatt
     2221 tttcatttta ttcaacatcc ttaaataaac ttccgttcct aagctctata atatcttctt
     2281 aaaaaataat attatggtgt cgttttcaaa ctcgtaatct tttacattgg aaaaatacat
     2341 acttttctca atgtagcaac tcctatctca cagtattcaa aaattcttca atattatgaa
     2401 atacttgtgc atgttcttta aataccttgt cagtctgttt ataatcccct actaaatttt
     2461 gcattgctcc caactcttcg acaaattgtc atctccatct gtaaacagcc ctaaaaactc
     2521 atataatgct ttttcatcaa tatttttccc atattattct cggtatagtt tcacaagttt
     2581 tacggattct tcatattgtt tatcttttta taatattcat ctagggacat aattactttt
     2641 gctagatttc ctgcaacaac acaatcgtct gatacgtcct tgtttactaa actatttgca
     2701 cctataacta cattatttcc tatatgtaca ccctttagta ttctagactt cattcctata
     2761 aaaacattat tacctatttt gatcctctcc gatgatccca aagcttctcc atactacttt
     2821 taaaaccgat caatcataaa catgagtcaa aattgtaact ccttcatatc atctccaatt
     2881 tcaacaagag tcttcgttgg aacaaaaact atacatcgtt cgcctattcg gcagcctaaa
     2941 aattgtagtt actttatata tcattctgat gtctcttctc atctttttac caataccgac
     3001 gtaagaactt tcaaaatata cttaaacttc ttattacgga tattaaacag aagaataact
     3061 aaaatcgata tgacaaccga tataactaaa tttatcacta aacttatcac tttgaaattt
     3121 ccgatattaa catacgaaca agcaaaataa cttaaataag aagcaacaaa agctattaca
     3181 cacaaactta tgagcttcca aacatattta cctgccgatt caaatttaaa tatattttgg
     3241 aaaacattat ataataacca gggtaaatca accaacaata ctgaaacaat ggttgccgcc
     3301 aaaactccat ataatccgaa ccaattaacg aggatgatat ttaatacaat gtttatcatt
     3361 gctgtaatta atggtctaaa cctgtctgcg taccatattc cagcagcatc tttgtataac
     3421 ccaattaact gatcaatttc atataagaaa aaatgaacta tcataaatat aacaaatgaa
     3481 atactcataa gattatcact tcccacccag ataatcatga acggctgaaa taatgtaata
     3541 aagcatgcac aactaattgc cagaaaccaa cctataattg ttgtaatatc acaaaaatct
     3601 atataatttt tttcttgact ttctacaata agactgttac ctatacctgc tatacatgat
     3661 tcataaatta ttgcaaatat actcattatg gcagaaataa taaagtaata attctgataa
     3721 atcgcaagta ctgaaagacc taagaaagat gaaataataa ttgaatctga agatcgtaat
     3781 attgtaccgc ctaccttgtt tgtaactaat ccttttactt tttttataat atcgtcaata
     3841 gtttctttct ctaactcacc aacaggagta taatcaggat acatcttttg agaaacaaca
     3901 gcacacatta agttaagaac tatttgtccc aaaatagaag ccaataaata caaatagtaa
     3961 ttttttaaga aaatcagaac atatgcctgc attacaaatt ggaatgtaaa agtaattagc
     4021 atagacacgt taattacatc atttctttga tgtgcatata ttagactctt tctatatgaa
     4081 aataaccaat acgacaaaac agtatttatc aaatacatta aatacaaaac tgtcatattt
     4141 aaatttccag gaatcgaacc tttaactaaa aatctcaaaa aaggaatgca taacaatcct
     4201 aatgtaaaca ctatccatcc aataattgaa aagtactttt tatatgcgtt cagtaaagca
     4261 caaatctctt ttctattgtc ttctgctata ggtttataca tacttacagt tagtgcagca
     4321 cctattccca attccgctaa atttaacatt tgaaatatag atgtgaacag gttgttcaat
     4381 ccagcatact ccattcccat ctgataaatc ataagtgtac gcataaaaaa gggaatgata
     4441 atctgatata acttaaaaat tgttccagta attatattcc ttttagcatt ttgtttacga
     4501 ttaattatca tatcatatgg ctccaaactt tctatttttt attgttacct aatactatat
     4561 aatttctaaa atcttgaaat gtaataggtt tcttctagct ctttgattaa ttacgctaat
     4621 tttcatgttt ggacaaccac aaaaaactat acattttcaa tcaaagcaac ccattgtttt
     4681 gtaatttctt tcaaattaaa tttttgtaca ttattatatg cgttcagtga cattctcatt
     4741 gctaaatcag tattcttagt aaacattatc atgctcttag ctaattggtc tatattctta
     4801 tcttcaacca acatcccatt aaaattatga tgtaccaaat ctctcggacc agttttacaa
     4861 tcataagaaa taactggtaa tccataagac tgtgcttcta atataaccat aggaaatcct
     4921 tcataaacag aactaagcac ataacaagat gcagatttat aatattcctc cacatggtct
     4981 gaatgccctg ctatagttac tctttgcaga tttaaatctt ttatctgctg gcgaagatct
     5041 ttttctaatt caccttttcc tacaatgata agatcccaat catctacttt tttttctaca
     5101 attttccaag cacttatcat gtcagcaaaa tttttctctt ccactaatcg ccctactgca
     5161 agaaatctct ttttatcata atgatattcg tactcgatat tagatataca cggatttggc
     5221 atagttataa caaaatcttc tgataaacta tattcatcaa tatgtttttg acgatcttct
     5281 tctgtgagaa ccacaagcct ctttgcatat ttttttatca attttttagc cttgaatctc
     5341 ttcttttctt tcagcatttc cattgtccag taattaaaat gttcccaaga aatcaatttg
     5401 cactttgttc ctttaattgc ccttgtagta tacaaagaca acaaagtatc aatatcaatt
     5461 actatatcaa attcattttt gcatatatac ttgtgcaatt tccaaaccgg atataaataa
     5521 gttctatata attttccctc cgttttagga tttaacatat aatcaacctt aatcgtctta
     5581 tctaacccaa aataaggctc tccactattc caaaagctta aaatagtgac atcataacct
     5641 aaatcagaca agctattggc aataatactc cctactcttt cagttccccc agaacgagaa
     5701 attacaccac taatgaaaca aattttcttc aaagtacttt tctcctttat aagtgtaaaa
     5761 attttttcag tgcaagtaac ctgttaaatt ttttttgtcc aattgttgat tttaatctat
     5821 aaacaaattt ttgtttaggt gttttccaag atgcattata ttgatgaatg cttattgtat
     5881 tactagtttt ataaattttt ccagtatcat aatccttggc agcaaaataa tccgtaggat
     5941 agatatgcag gccatttatt atttgttcgc tgttatcgcg aaccaatcca atagattcaa
     6001 gataatctgt aatataatac ggactcggtt tcaagttcaa tgtaccatca ttttgataaa
     6061 aacttaatga atcgtaaata tcccttaaac ctttaataac agaatgtcct tcttctgcac
     6121 caaaaccaat accgccatta acccagattt ccccttcaaa tcccatatag gcatgatttt
     6181 cacgaagtat atctaacgac ttaattagct caacatctgt atctaaataa aatccaccat
     6241 gattataaat aatatcaaat cttgcataat ctggaacaaa accccattta cgttctttat
     6301 atgcatcggc catataacgg ttcttattta aatcataatt tgattcattc cattcaataa
     6361 tctcataatc agggcaatac tttttccagc tttctatgca ctttctatct tttttcggaa
     6421 gtggatttcc cccgaaccaa cagtaatgta tcttcttagg aatcataata tctctccttt
     6481 tgtatttttt tcactataat aatgctcttt ccttttcaca acttcaggca tggctaaaat
     6541 catataaatc agtggccccg ttgtttcacc cctaagtgtt gtgttataca caaaaaagaa
     6601 taccgacata attgcaataa ctttaacaag acttgcatct ttatccttca taaaagatct
     6661 ttttgcattg aaaaatgtct ttattataaa aaataggaat atcgaaactc ctaatattcc
     6721 aacttctata aataaccaaa tatgatgaaa atactgataa tgaagattac caaacgattt
     6781 ataaaactca ctgacaaaaa aattggtaaa ctctgcattg ccaatgccta ctcccattag
     6841 gactaatcta aaattattat taaatatttc atctactact ctagagatac cagttattcg
     6901 atttaaatca ccaacaccat catatccaga atctcttgac gcatattcta aaagaccttg
     6961 aattgtgaaa ttatcagcat agctttgtcc attatcgttc aatgtaataa atattgttat
     7021 aaaaaaggat aatactatcg acacaagtat tacgcatgat aatatcttga tcactacttt
     7081 aaaatcaact tttaaagtga atactattgc aacaaatatt ataaataatt caggaataaa
     7141 aactttcaat tcagctaatg cagacataaa aagtgctgca caaacacata aaataaaatg
     7201 gatgaaattt ttttctccct tgaaatatcc gattgtaaca acagtaactg tcacacacaa
     7261 aattaaattc aatggcatat tacaacctga ataattccaa aaaactccac ctatcatatc
     7321 tcctacaatt gttccagttg gataggtgac catgaatctt tcaattaaac aaagtggcag
     7381 tgaaatccag aataattttt caattatagc gaatacttta tagtaatctt taattgttaa
     7441 atatgttgca caatttatat atactatgaa catacgagtg ttatttcgta tcccccagat
     7501 aatcaatcct atattttcta aattaataac tgagctacaa atagaaacta gtaaaaacaa
     7561 aactatcagc atctcatttt tgctagcata tattcctttt ctgcgtctaa agctaagtgc
     7621 atttaataac acaaagatat taattatatc tcccaaatat aaaattgctc ttggggcgtt
     7681 aaaagtatat acaagaacta tggagattgc aaaaaatgcc aacgtgaaca atgtccaatc
     7741 tgctcctgac catcttttta ttgaaatacg catcgttatt caccttcttt cgctaaaaat
     7801 tattctaaaa atacttttgt taaattaaca taccctatta tttttgccgg tacacctgca
     7861 agaattgcac cattctctag acaatctttg ataacaatag ctcctgccgc aacaactaca
     7921 ttgttagcta tttttacatt accaataatc tttgcaccca cgcatacacg tacattattt
     7981 ccaattacag gacacttagg atcgattcca tcatttccaa tacagttatt cccatataag
     8041 taacaatttt ttcctattat ggcgttacca ttaataacaa ttccttgtgt atggtaaaat
     8101 cttacacctt catcaaatac attttcacca cactctatac cacattttct tcccaaacta
     8161 ttcattcttc tgcaagacac aatatacata atcgcataaa tgatattttt ctttctcttt
     8221 gaataataat aagatgtaat tctcattcga cgagtatacc gccaagcgtt gtagtccgga
     8281 tgtgatttca ttatttttag taattttgtg tgttgtcctt tagattctat atatagattc
     8341 ttttccatac gaagaaccgc ttttaaatat tctttactgc taatttccat tcatatctcc
     8401 tattaattca atttatactt aacaatttta ttcggatttc ttgcaacaag agcatcattc
     8461 aatgctactg aatctacaac gatggatatg ttatcttcat cgttgattcc accggataca
     8521 atattactta taaaaatcgt aacatttttt ccaaaagaag ggatgtccgt gatgtataat
     8581 gcccattttc cgatatttcc agttatttaa acctaacgaa tttttacaat agtacaagta
     8641 tcacaatcat taatatataa ctggaatata aatctccaaa aattcactat agtacagttt
     8701 tttatgatta aaatctccta tttctaccca ttccgcttaa atatgctaat gttttaaccc
     8761 caataatgga ttggtttagt aaaaaatagt taaatattcg ttgtttaatt ggattttgtt
     8821 tatatgggta tgagtgaata caggcaatca actcatcgtt cataagtatc ttagacaaat
     8881 aggagtttaa atcaaattct ttattaaaac tagcatatct aactgccttt tgaatacaag
     8941 tacgacagcg tcccaacaaa agtctccttg ttttgtttac aaatatctca ttttctaata
     9001 tatagcttaa atcgccttca atttttttaa ctaattcaat attacaatcg aactcattat
     9061 ctctaaaatt atgtgtcaat gaattattat tattacatct ataataatag atacaatctt
     9121 taataaaact tgttattttt gaatgttgca aatatgaaat atggaatatg atatcttcag
     9181 atatataatc cctttctgac ggaaacttga tattgtgctg ttgaattatc tcagatgaat
     9241 acagcgcatg ccaaactgac attggcaata taaaatcgac tttataactc ggctcagtgc
     9301 caatcattcc aagcaacact ttttcaatta tagcgttatt tgtaaatatc gtactatcat
     9361 atcttgctgg ctttgaaata atattattat catcataata ttcataatat ccagcataac
     9421 atgtgtctgc attactcctt tttataccat ttaacattgc ttcaatataa ttagctgcaa
     9481 caaaatcatc agaatcaata aaagcaacat attttccttt tacatgttca agtcctgtat
     9541 ttcttgccaa tcccaagcca gcattatttt tatgaataat acgaatcctt gaatccttta
     9601 atgcatagtc ttcacatatt ttattagatg aatccttaga tccatcatcc acaattataa
     9661 tatctaggtt cttatatgtt tgattacaca ctgattccaa acaacttcct acatactttt
     9721 caacattata tacaggtatg attacactaa ttagatcact cgaattcatc cttctatcct
     9781 ttcactcacg ttttctcaat tagtcaatga cggcatcaac gattttctta aattctctga
     9841 aaaaaataga ctctgatctt gatgatgtga atgacatact atcttcttct agaaaaatta
     9901 tttcctttaa attatttatt aaatcaataa tatgatcctc gtaagtatgc tttcccttgg
     9961 aataatgaaa atatactttt gtattacaca atattgcaat tttatttctt aatatgatta
    10021 tttcactcat tcattactgc cacatataca caacttataa ccaccccctg caaagtttag
    10081 ggtaagttat cagataatat cttcctcttc atcatccaga atacaaaatc tatttattta
    10141 tagaaatatc attaatgttt ttatataatg taccgtgctt gtactcttgc tctattacaa
    10201 gcactaaaac actactattc atttgaattc ttacacggat aaaaatttac atcgtctatt
    10261 tcaatatttt ttctctctat tttattcact taataagttc attacataat cgaaacattt
    10321 tttattttcc ttgtcatcac tccttgctcc ctttttgata cagatttaaa attctatcaa
    10381 agaaatttcc atcgttaaat ttcgttattg ccaaatcctt cgaacaacgt gaaaatttta
    10441 cgtatttatc tttatcattg atttttagta ttgcagatgt caacgctgat acgtcaccag
    10501 gttgaaacaa atatccattt tctccatcac gtactgctga agaaatatct cctacatctg
    10561 tagcaattac aggcattcca taagaaatcg cctctagaat agatataggc aatccctcgt
    10621 tataggacgg taaaatcatc acatttgatc ttctatagca ctcctccttt ttttctccca
    10681 caacccaacc tgtaaattca aaatacgatt gcaaattata ttcttctatc aattctttga
    10741 gcgatatttc ttcatcacct gctccagcaa tctcaaaaat aaactttttt tctggatatt
    10801 gttctgcaat gtttttaact gcttctacca aatcgtgtac accttttcgc tttatcaaaa
    10861 ctcccataaa cagaaatcgg cacatttttg aattccactt tatggtatat tctggaacta
    10921 caatagaatt ttctacaaca acagttttac aatttggctc aatttcttta attatcttct
    10981 tccatttttc tccaagtaca atcgtggaat cagactctct taacagcaag cgaatctgat
    11041 tttgtttttt tacagaaaca ctatcatacc attttttaaa ttccgatcca tgaagatgga
    11101 tgattacagg aatatttaat aatctaaacc acttattcag cacatatttc ctactaaaac
    11161 tccctctgta cgacatgtgc atatgaataa tattaggttt actcaataaa gagataccta
    11221 taattttagc aaatgcatac ataaaatata aagatttaaa tattgcattt ccacccttat
    11281 aactcggtat aaaagtcatc tcgatattct ttttttccca attaaaattg agcatttgtg
    11341 taattatact tgtaattcca ccttttaccg agctgtcatt accaatcata agtactcttt
    11401 gcataacgct ttactcctta tatttatatt ttttctatta atacctctat cgaattaata
    11461 atcgtttccg tgttagatac atacttgtta aacatccgaa ctcttgtttc tttaacggca
    11521 tcttctaaat tttctaattc ataacaagga tagataatat tcatctcatc aaattgtcta
    11581 attaactgca gttggtgatc atcgacatgc tcaccatact tagccaatcg aggaacagca
    11641 atgacccttt tacctttttt tacggcccca attatagcac ctgtacctcc atgagtaatc
    11701 accatttctg ctttttgcat agtttcagta aactcatccc tatttagaaa tgctttatag
    11761 gaataattta gtggtttata atcactataa ccactctgtg caaatgtact ttcgattatt
    11821 actccttttt caattaaatc atcaatagct tttaacaatc tatcaaattg aaatttttgt
    11881 gaacctaatg ttataaatat catttttttc tcctattatt taatatatcc cacccaaata
    11941 aatagcatta ggataaaatt gtagcatagt ttcccactga acataaaatt gatctgcaaa
    12001 atgatacatc aattttcctg tctgagtagg gctggttact tttgcaaacg attcaatata
    12061 aatcagtttt tttcccatca tttttgttat taagcagata gggatcatag ctaaaacacc
    12121 tgtacaaatc acgagatccg gtttttcctt tatatatata ataattgatt tgaataaatt
    12181 aataagtata gtacacaaaa aaaacttttc ttttctattt acttgtctca tgtagtacgt
    12241 tttttcgcct tttgactcag ctgaatattc tgtctgttct gttataacaa aactctcata
    12301 cttctccatc agtggtttta acatcattaa ctgttcataa tgccccccag aggaagccgc
    12361 aaaacatatc tttttgttca tattaacctc tctatcagtc acgctgaaaa atatctctag
    12421 tgtaaacttt ttctttaata tcatccaagc aggtatcata tcgattggca ataatagaat
    12481 cacacatcgc cttaaactgc tctaaatcgt tgataacctt acttccgaaa aaagtctctc
    12541 catctttcag tgcaggctca taaatcacca cagtcgcacc tttagctttt acgcgcttca
    12601 taactccctg gattgaactc tgacgaaaat tatcactgtt gctcttcatg gtcagccggt
    12661 atataccgat taaaactgga ttttccctgt tgacatcata ctgattatat tcagcgtagt
    12721 tgtaatagcc cgccatctga agaacacgat ctgcgatgaa atccttacgg gtacgattag
    12781 attcaacgat agcctcaatc aaatttccag gaacatctgc atagtttgcc aacaactgct
    12841 tggtatcttt tggcaagcaa taacctccat atccaaagga tggattgttg tagtgagttc
    12901 caatacgagg atcgagacaa acaccattga ttatctgctg agtattcagc cccttcattt
    12961 cagcataagt gtctaattca ttgaaatagc tgactcgcag tgctaaatag gtatttgcaa
    13021 acagctttac tgcttcggct tcagtgaatc ccataaaaag agtatccacg ttctccttaa
    13081 ttgcactttt ctgcagaagt gttgcaaaaa catgagcagc atttaacaag cgctcatcag
    13141 tcatatcagt accaacaata atacgagatg gatacagatt atcatataaa gccttgctct
    13201 cgcgcagaaa ctctggacta aaaataatgt tcttactccc cgttgtctca cggactgatt
    13261 cagtgtagcc aacaggaatt gtagacttga tgaccatgat ggtatcagga ttcacgtcca
    13321 tcacaacctc aataactgac tcaacagcag aagtatcgaa atgctgagta cgactgtcat
    13381 agttagtcgg tgtagcaata acaacgaaat ctgcggattt ataagcgctc cttgcatcca
    13441 aagtagcagt taagtcaagt tctttttctg taagatactt ttcaatatat tcatcctgaa
    13501 ttggagactt ccgtttatta atcagttcaa tcttttcagg gataatgtct accgcggtta
    13561 cctggtgatg ctgcgccaat agtgtggcga tactcaaacc aacataaccg gtgccggcta
    13621 ctgcgatgtt gtactttttc ttcacaatat ctcctctttc ctctcttaag tatggagaat
    13681 gttactccta cggctattca tcatgtcaaa ataacacagc ttagaatgtt gataaaataa
    13741 ttttccagaa tgtgataaaa tctctttcat ttataaacca cacatctaat tttatctatt
    13801 ctccacaaga catgtagagt attctttaat aacaagatac gaccttacta aatacttcaa
    13861 aaaattatca tatttaattc ataaatataa atcttcattt taaatgtatg cccaaaaatt
    13921 tatctgtaat gcgtcatcaa aatctagatt ccaaatcttt atcaattttt atccactcat
    13981 tctaccactt tagtaatatt ttctatttat aacactttcg tcctacattg atccccccct
    14041 ctgaagtaca gtttttactg tcttaaataa aattttaaaa tctaacccca tactccaatt
    14101 ttttatatat tccgtgtcaa gttttgctac ttcttcaaaa tcagtaatat cacttctacc
    14161 acttacctgc cacattccag taattcctgg cttgatagca attcttgcac gatgatgata
    14221 atcatattct tcatactctg atttaaggca tggtcgaaat cctataaggc tcatctttcc
    14281 tgttagaacg ttgaaaaatt gagggaattc atctaaagac gtggatctaa taaattgacc
    14341 gattcctgtc ttttttgttc catctggaag tattttattt ccgataattc ttggatcaaa
    14401 ctccatctta aacataagat tattgctcat cttattttct ttcattaatt ctacttttct
    14461 ttcttctgca tccatataca tacttctaaa cttatacatc ttgaacggtt taccattctg
    14521 acctatacgt tcttgtgcaa agaaaattgg gcctggtgaa ttaatataaa taagtggagc
    14581 aataaagata aaaataattc ccgttgcaat acatccaaca atacctgcaa gaatatcaat
    14641 tgttcgtttt ataaatgcct gttgagttgt cataacattg atacttgttg taagtactgt
    14701 atattctcct atacgaccaa tagattgttt tcccaaagca ctatctgata cttttgcaag
    14761 attcaaatgt acagtaaggc ccatttcaga gcatcgttca ataaattctt tcatacacga
    14821 agtactttta ttcaaaataa taaagacttc atctacccat ccctgacata agtaatccgc
    14881 tgcatttttg gaatcagaaa caactgtcac tccctcaaca tcttcaccaa tcatgctttt
    14941 atctatgata atgacaccat ttattttata cattttatag ttatgctctt tcacattacg
    15001 aataactact tcagcaatac tactagatgt aactataata agtgatttgt tcccctcatt
    15061 tttcatttta tgtttcaagt aatgtttcca taagaggcga gttatatatg taattacaac
    15121 gtataaacct cccatcaaga aaagaacgac tctagaataa tcatttccac cctgaacact
    15181 aatgagatac aaacttccta acagcaccaa cataatcgct tgctttacag aagaagcaaa
    15241 ttctatatag taccctcttt ttaatacacc tctatatgct tcaaacatga aaataactaa
    15301 aagatctgcc agttcaatga agactgccat gttttgatac attggaatca cataaggatt
    15361 ccaatctacc tggcgtaaga catacgatag ataaaaagca agctggatac acaataaatc
    15421 taaaataata aaatcaatat gtttaaccca tccccttgaa cttttcttgt acattccttt
    15481 ttactcccct ctttttaata aaataaattg tattacttgt tttctctcct aaatatcttt
    15541 ctgaaggatt attgactcga caaattggct tgtggatatt cttatatata gaacatccgt
    15601 aagccaactt aaaactcctt aacaaaattt ttttactcat attgtagatc aaaacaagta
    15661 tccaagaaat catattttat ttcttttttc tgcttactca acaacagcta aaagaaaaat
    15721 catcataaac aaattcaact aagccaacga tccccttgtg aaatcaagtt ttttcttgat
    15781 ttacgaataa agagctaaaa taaaccaaac ttcttctttt ttacctcttc atagactgga
    15841 taactgattt catccccgtt gatcagatcc tttgcgactt gtttcatctg tactactttt
    15901 tccttaccaa agtcctttgc aatctgctca tagcactctt ttaagtaaaa ggtgcgctta
    15961 gagaccccat gggcatcaga agcagccatc tgcacgagat tatgtttgac catcgttttg
    16021 gctgttttct ggatgtcttt gccaaatacc ccaacataac ttggcgcagt gagttgggcc
    16081 aaacatccca tatctaggaa cggaagcaaa tgatttgggt cttcgcgaaa atgcacgttg
    16141 cgttctggat ggacgattac tggtgtcttt cctagttcta acaactcaaa aaaaagcc
//
LOCUS       DACCCS010000064        15296 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.065, whole genome shotgun sequence.
ACCESSION   DACCCS010000064 DACCCS010000000
VERSION     DACCCS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.065"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            278..787
                     /locus_tag="IVK82_002212"
     CDS             278..787
                     /locus_tag="IVK82_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6393257.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IVK82_002213"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IVK82_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP6393258.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IVK82_002214"
     CDS             2322..3302
                     /locus_tag="IVK82_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6393259.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3407..4825
                     /locus_tag="IVK82_002215"
     CDS             3407..4825
                     /locus_tag="IVK82_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP6393260.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4896..6365
                     /locus_tag="IVK82_002216"
     CDS             4896..6365
                     /locus_tag="IVK82_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6393261.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6375..6842
                     /locus_tag="IVK82_002217"
     CDS             6375..6842
                     /locus_tag="IVK82_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6393262.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6878..7750
                     /locus_tag="IVK82_002218"
     CDS             6878..7750
                     /locus_tag="IVK82_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6393263.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7832..10366
                     /locus_tag="IVK82_002219"
     CDS             7832..10366
                     /locus_tag="IVK82_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6393264.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10522..11295
                     /locus_tag="IVK82_002220"
     CDS             10522..11295
                     /locus_tag="IVK82_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6393265.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11288..12079
                     /locus_tag="IVK82_002221"
     CDS             11288..12079
                     /locus_tag="IVK82_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6393266.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12089..12580
                     /locus_tag="IVK82_002222"
     CDS             12089..12580
                     /locus_tag="IVK82_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6393267.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12598..13008
                     /locus_tag="IVK82_002223"
     CDS             12598..13008
                     /locus_tag="IVK82_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6393268.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13012..14412
                     /locus_tag="IVK82_002224"
     CDS             13012..14412
                     /locus_tag="IVK82_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP6393269.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14435..14809
                     /locus_tag="IVK82_002225"
     CDS             14435..14809
                     /locus_tag="IVK82_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP6393270.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5309 a         2154 c         3055 g         4778 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatattag ttgtgaagat aaaaaccttt tcactggagg aagaaaatgg agaaattatt
     3421 aattaaaagc aataatcatc aactttacta ccaacctaag gatgtttggg tcggagatgt
     3481 tatgccttac gggaaagaag gaaaattctt tatttatcat caacgtgata ctagaaatcc
     3541 agttcctttt ggagaaccgt ttggctgggc tttagcaact acgaaagatt ttgttcattt
     3601 tgaagacttt ggggaatctc ttttaagagg ggatgacaat gagagagacc agtttatcta
     3661 cgctggttct gtatttgagg caaatggaaa ggttcacgca ttttatacag ggtataatcg
     3721 tgaattctta gcgcagggaa agacatctca agtgttgctt catgctgaga gtgatgattt
     3781 tgttcattgg gaaaaatcag aaacagcatt ggagttagaa ccacaagaag ggtatgatat
     3841 tcgaaactgg agagatccat ttgtcgtttg ggatgatgaa aaagaagaat atttgctaat
     3901 tttaggggcg cgaaagggtc aggataaaca tctacaaacc ggacgtttag tggaatttac
     3961 atcaaaagat ttaactaatt gggaattcaa aggtgatttt tgggctccgg gattgtatac
     4021 catgtttgaa atgccagaac ttttcaagat tggtgactgg tggtatcttg tctattcaga
     4081 atactcagat ggaaataaaa tactctatcg aatgagtcaa agtttggaag gtccttggat
     4141 taagcctaaa gatgaagctt ttgatggacg ggcttattat gcaggtagaa cagcttatga
     4201 tggaaaacgt cgagtcctat ttggatgggt accaacaaaa gatcaagaaa atgataaaaa
     4261 caattattta tggggaggta ctttggttcc tcatgaagtc tatcaaaaag aggatttttc
     4321 acttggggta aaacccatag acgaagtgtg gaatgcattc ataaatttac atgatattgc
     4381 cgatgtagag atagaaacag cagacacaag agaagaagta acgcttgtca aaaatagtgg
     4441 aactttgttt aatttagaaa caacaatttc atttgatgag aatcttagtg acttttcact
     4501 gagattatat aaaaaccttg agacagatga atcttacgaa tatcgcttca atttagatga
     4561 ggggcgtctt acatttgata aaaacccttg ttacccttgg tttcaatata tggataaagg
     4621 acttattaga cctatatctt taaagcctaa ccaatcttat aaactaaaag tgattgttga
     4681 tgacaatata atgacacttt atattgatgg aatcgctctt aacgctcgtg tacatggttg
     4741 ttttggtcaa gcaattacta ttacagcaac aaatggatca atgactgcaa taaataccaa
     4801 gttttcaaat gaaataaaga aataataaac gtcaatcatg tggcaggtct gaaaatcaaa
     4861 catgacctgt cacatttaaa agggaggata aatgaatgga ctacaaaaaa attgccgaag
     4921 atgtattgga aaacgttgga ggaaaagaga atatctcaag cgctacacac tgcattactc
     4981 gtttacgttt aattcttaaa gacaaaagca aagcgaatat ggaaatgctt gaggaaatag
     5041 aaggtgcaaa aggtgttctt ttcaatagtg gacagttaca agtaattttc ggaacaggta
     5101 taattgaaaa ggtttacaaa gagttcgttg aattgacagg agctaaggaa gtatccgttt
     5161 ctgaaatgaa agacgaagga gtcaataatt caggaaatag acttcaacga gcttttaaag
     5221 ttttctcgga tatctttatt ccgatcattc cagcttttat tggggcagca atggttttag
     5281 gaattcgttc gttgattacc actcctggaa tgtttggaat ggatggctca ttcgctgatc
     5341 acaatgtatt cctaaatgat ttatccaaat ttctgggtat tattgcaacc acatttgatt
     5401 atttaccagt tttagttatg tattctgcaa caaaacgttt tggcggaaac cctattttag
     5461 gattacttgt aggatttgtt atgattcatc caggactagc caatcggaat gatttcgtca
     5521 tgggaactat aaagcctgat tattggcatt tatttggatt aagcattcca gcagttgcgt
     5581 ttcaaggggg tgtttttcca gcaattttga cttcttggtt tttagcaaaa gttgagaaaa
     5641 ttacaaataa atattctcct caagtacttg cttacatttt agtcccaact gtgacaattg
     5701 ttcttgccaa ctttgcacta tttttagtgt ttggtccatt ggggaatttt atcggtaatg
     5761 ttttaggtgg aatcattgat ttcctatata tgaagatggg tgcctttggt gcttttgttt
     5821 ttgcaggcgc gttacaacca ttagttgtaa caggaacaca acatgctatt caaggtattg
     5881 aggcaaactt agtcgcaaca actggattca attatatcca acctatttgg tcagtttcaa
     5941 ttattgccca aggtggcggt tgtattggta tgtatttact ctttaagaaa aaatctaaag
     6001 atagagatat tgcgatttca agctttattc caacactggt aggtattact gaaccggcta
     6061 tctttgctgt taatttgaaa tattcaatcg ttccttttgt ttgctcgttt attggtgctg
     6121 gttttggtgg agcatttatg aaaatttttg atgtaaaagc cattggacaa ggattgactg
     6181 tattgccagg tctgactatt gctaatccag ttcttcctta tatatttggt aatctcatag
     6241 cattcatttt accaatcatt tttattctcg cttatgataa atataaagtg tttatccctg
     6301 aagctaaaca ccgtaaattt gcagaaaaaa ctgactcaaa aaaattgcca gttcaagtaa
     6361 agtgagatga gagcatgtta aattttttta gaaaaaatga aataagcgtt gtagcacctg
     6421 caaccggtgt ttttgaaaaa ctggagaaat taagtgatcc agtcttttca aaaggaatga
     6481 tgggacaagg atttgcaatt gatgcaaaaa acgaaacgat tgtatctcca atcgatggtt
     6541 ttgtttcaag tatttttcct acaaaacatg ccttaggaat aaaaacaaaa tcaggaattg
     6601 agatacttgt tcatgtagga atagatacag ttgagctaaa tggtgaggga tttgatatta
     6661 aagttcaaga aggacaaaaa gttaaggctg gggatattct tatgaccatt aattttgtag
     6721 tttttgaaaa gaatcacaag actaaagatg tcattgttgt ttttccagac taccaagacg
     6781 aaattttcat tccgaaactt gaagaagtaa aacaaggaca agaattgttt gttcttggct
     6841 aatgataagt aattaataat agaatcgagg aaaatgtatg gtttttgggg ctattgaagc
     6901 tggtggaaca aaatttgttt gtgccattgg agataaagat ttgaacatat ttgaaaaagt
     6961 cagctttccc acgactacac ccaaagaaac aatgaattta gttttagatt tttttaatca
     7021 tcataaagat gatttagaag gtatcggagt gggatcgttc ggtcctattg atattcattg
     7081 tgaatctaaa acgtatggct tcattacatc aacgccaaaa cttgcttggc aggattttga
     7141 ttttattgga agtttaaaaa agcaacttaa tattccaact gcttggacga ctgatgtaaa
     7201 tgcctcttgc tatggtgaat atgtttctgg aaatggcaga ggcctttcaa gtgttgttta
     7261 ctataccgta ggaacgggca taggaggtgg cgctatccaa aacggaaact tcattgaagg
     7321 ttttagtcat ccagaaatgg gacatatgct tgtaaaacga cacaataaag acaacttttc
     7381 tgggacatgt ccattccacg gagactgttt agagggctta gctgctggtc cggcaatcga
     7441 aagaagatca gggaaaaagg gacaagaagt gactgaaaat gacgttactt gggaaataga
     7501 agcttattac attgctcagt gtgcatataa tacaacattg atgttatcac ctgatgtcat
     7561 tatatttggt ggcggagtaa tgaaacaaaa acacttaaaa aagaaagtac aagataagtt
     7621 tattgagttg atcaacggat atgtcgctat tcccgatgtt gacaattata ttatgacacc
     7681 aaaattgtta gataattctg gtattgtagg ttgtttagct ttagcaagag acgttaaaga
     7741 aaaatcttaa tttggaaaaa agatcgtctc gactatttgt ttggttgatg cgatcttttt
     7801 tttcaattag taattgaagg gacgcctaaa agtgaaaaaa aaaatgaaag atgaagtatt
     7861 agagtatatc aaaaataatt taaagtatta cgatttttct gctcaagata ttgcaatgaa
     7921 attttgtatt aaaagaaatg tcgcttccca ctatttgaat cagttatttt cagatggaaa
     7981 attactaaag aatgattctg ttagaccagt aatgttcaag tataatcaac aaaagccaaa
     8041 agactgcttt tctaagttta ttggagcaga catatctttg aaatccacta tagataaatg
     8101 caaagctact gtaatgtatc cccctaatgg cctcccatta ataataaaag gaaacagtgg
     8161 cgttggaaaa agttttttag caagtttgat ctatcagtat gctcttgata gaaaagtaat
     8221 tcataatgac gcaaaatttg ttgttgtgaa ttgtgcagat tatgcaaata atccagagct
     8281 attgtcagca gttttatttg gatataaaaa aggagccttt actggggctg aaaaagatac
     8341 tgcagggatg ctatcttctg ctaatggagg ataccttttt ttagatgagg ttcacaatct
     8401 aagtgcagaa aaccaagaga aattgtttct tcttatggat tctcaaaagt atcgtaaact
     8461 tggtgaaagt gtgcaatggg agtatgcaaa tgttcgcctt atcttagcca caacagaaga
     8521 taaaaatacc tctttgttag caacttttag aagacgcatc ccagctgaaa tcactctgcc
     8581 agattacaat aatagaagta catctgaaaa aattcaatta ttatttgaat ttcttaaaga
     8641 tgaagcaatt acaattgatt caaaaatatt ttgttcgatt gaacttttta ctgatctttt
     8701 atcgaaaaca tttgaaggaa atgttggaga attgaggaat gaaatcaagc ttctttgtgc
     8761 agaaggttat ttaggaaata agagaaagtc ttcaatttat ttaggggaca aactagacag
     8821 cggtttttgg atagatccag aaattagtat agatttaaaa aaaatgtttc ttaaatcact
     8881 ttctgaaatt gatttgattg aactttttca aaatcacatt tcaattgaag aaagcatgag
     8941 tcaattaaga gatagaatac ttaaagagtg tgcaggttct tctgagtata attatttaga
     9001 aagtgacgaa tttatagcat taagaaacta tgttgttaat aaaatatcac caattattga
     9061 ttcgacaggt ctatgtttat tagatgaatt cttggcggat atcagtctat ttataatgtt
     9121 tattgatgta attgaagaaa catcaagaaa ttttttgctg tctaagttca gaaaatttcg
     9181 tatgaaaaat gataaacaaa aattattggc aaatgagatt tggagtagtt tagaagtcga
     9241 agaaagtaaa caagaacttt tgttgaaatg gatacttttt cttgtaaaga agttctatgt
     9301 gaaaatacca gaaacccatt gtctgatagt tatgcacggt aaaataacag cttctgcgat
     9361 cgcaagggaa acaaataagt tgctgaatac atatgtttat gaggctttcg atatgccaat
     9421 tgaaggtgag actagtgact taattaacct aatcaacaat ttttgtaaaa gtatagatac
     9481 gacgaatgga ctcattctgc tagttgatat gggatcattg gaacaaatgt atgaaaaaat
     9541 tgaatccaat gtaataggtg atttggtaat tttaaacaat gtttctacag ctttagcgat
     9601 tgaatgtggg attcaagttt gccaaaaaaa gccaattagt catttttacc agatggattt
     9661 tgattcgttt caagttaaag ttcagtatta taaaggatta tcacaaaaga aaaatgtaat
     9721 agtatcttgt ctctctggtg aggggatttc tgaaaaagtt aaggatattt taaaacggta
     9781 tctcgataat caagtggaga ttttgatttt tgattttaac gcattaaaga aaatagcaaa
     9841 ggaaaaagat actatgattt ttaagaatac aatttgtgtt ctttcaacca ctgaatttta
     9901 cattcaagga atcgattgtt taaatttaga aaatctaatt aatgggaatc aaaccttaga
     9961 aaaattaaat aagtatatgg actcggatag ctgtgagttc tgcttaaatg aattagttaa
    10021 actctttact attgaaggcg ctagtactaa gcttcgcttt ttagattcca aaaaagtatt
    10081 caatgagata gaaaaagtac tatatttgta tgaaaaatat tacgatgtca aaatcccaag
    10141 ttttctgaga attaatctat ttctacatct ttctggaatg attgaacgaa ttatgattgg
    10201 tgatggaata acaaatgaac atttgaaaga gggagcgcaa ctatttgata cttttttaaa
    10261 tgtatcaaaa gatttttttt cagaaatcaa agatatttac tgtattgaaa tacccaaagg
    10321 agagtatgag ttaatctata caatttttga gcagactatt ttctaaatag catactagcg
    10381 tctaaaaaaa tgaaagggtg tccaaagtat atttagacgc tcatttaatt aatatttact
    10441 gtattttccc gctttattgg ataatgttag ttggcacagt aattgcttat ataaaagtat
    10501 aagaagaaag gagagaacaa aatggcgttt tggcaaacat ttttagttgt gttgtatgga
    10561 tttttcatta actgggataa ggaagggccc aaaatcggaa cctcccaacc tgtcgttgca
    10621 ggtttcttga ccggacttat tatgggagat attcaaactg gactttatat cggtgggact
    10681 ttgcaattaa tgacactagg tattactagt tttggtggtg catcagtacc ggattatcaa
    10741 acagcagcat tgatcggtac ttatttagca atctcaacta accaggatgc ttctatagga
    10801 ataactttag caataccggt agcaatgtta attgtacaac tagatgttct aaagtggtca
    10861 acaaacattt tttttcaaca aaaggcagaa aaattcgcac aaaaagaaga ttatcaaaaa
    10921 ttagagtgga tgcaatattt ggcatcaatt aatacaagct ttgtatctgg aattcctgtt
    10981 ttgttaactg tgatattagg cccaacgtta gtgggaagtg caatcaatta tattcctgaa
    11041 tgggttaccg gtggtttaaa ggtagcaggg ggactattac ctgctgttgg gataggaatg
    11101 ctacttaaat atttacctac aaaagattac tttgcctatt tgttagttgg tttcgttttg
    11161 gcagtttatt tccaaatacc aattttaggg atttcgttac ttggcgctgc aatcgcacta
    11221 atcgtatata aaaatagatc aaataatact caagtagcac cagctgcaac aggaggtttt
    11281 gacgaagatg aataagaaac tgacaaaaat cgacaaacaa gtaattttcc ggtggattct
    11341 caatggtgca acttcattaa attatgaaaa aatggaaggg ttgggttatg cgtattctat
    11401 gcttcctttt atcaaggaca catataaaga tgatcctgaa ggaatgaaac aatccgtttt
    11461 agctcaccta caatttttta acactacgcc gtatacagcg ccttacatta ttggtatgaa
    11521 tgttggaatt gaaaaagaag aaaaaaataa gtctttggaa gcagtaagtg ctctgaaaac
    11581 aggtctaatg ggtcctttag cgggaattgg agatagtgta tttgtagtta ttccatggac
    11641 tatttttgga gcaattgcag ctaatatggc attggaaggt agttttgttg gaattatttt
    11701 gtggatactg gcatcaattt tattaaggat tactgcttat cctttatacc ttgctgggaa
    11761 agaatcgggt gcgagcctac taactcaaat tgaaacaaaa ttaaagtcta ttactgatgc
    11821 agtatcaatt ttagggttaa tggtggtcgg tgcactcatt gccacagttg taaaagtgaa
    11881 tgttggcttt gaatttaaac aaggcgactt aacaatgacc ggaaatgaaa tctttgatca
    11941 agttatgcct ggtttattac ctgcatgtgt agtagcaatt gtgtattatt tgttaggacg
    12001 aaaggttaaa ccagtttatg taattctatt aatcttagta gtttctattg cgttaagagc
    12061 gttaaatatt ttgaagtagg tgttgaatat ggatgaaaaa atgaaaggta ttatacatgt
    12121 tcgtattgat gatcgaatga ttcatggaca agtagcaaca caatggacaa atcaattgag
    12181 tgctaacaga attatggtta ttaataatga agtagccaat gatgacgtaa aaaaagctgt
    12241 agttcgcctc gctgcaccac ctaatgttag aacttcaata attacaaggg aagtagcagt
    12301 taaaaatatt ctttctggta aatatgaagg acaaaaagtt cttattgttg cagtttctcc
    12361 aatcgatgtt aaatttttga ttgataacgg tttaccaata acaagtgtaa acgtaggaaa
    12421 tttatcaaga aggaatggaa cagaacgcat ccgtccgacg ataaatatta ctgaagaaga
    12481 gagagaggct tttaaagaac taattagtga tggagttgaa gtgacagtga tacaaactcc
    12541 tagagattca aaagaatttt taaaggattt tatttattaa gaacagagga tgagtggatg
    12601 gagtttataa ttacatctca tggaaatatt gccattggat ttgtagatac attaaaaatg
    12661 ttttttggcg ataatttaaa ttgtcatgtg gtaacacttg gagatgcagg cattgaagaa
    12721 ttccgtgaaa acattgaaaa aatagtttct caagtaaaaa atcaagaagt tatggtattt
    12781 gctgacttga ttggtggaac acctttcaac gaatttgcta aaagaagtga agtatttcaa
    12841 aatgggtttc aacttcttgg tggcatgaat ttaggaattt tgattgaggc tgtcaatttg
    12901 agattacaag gaaaaaaaag cgatgatatc atcgaaaatc taagaaatat gaatacaatt
    12961 gcgtgtcttg cagatatgca aagacatact aacgaagagg atgagtgata aatgcaaaaa
    13021 attttttaca agcctaaaga tttttggttt ggggattgca tgccttttta taaagatgga
    13081 actttttatc tatttcatca acgagatacg agaaatccta aacctttaac tgatcctttt
    13141 gggtggtcac tagttacaac cactgatttt gtgaaatttg aagaatacgg ggaagtaatt
    13201 catcgtggtg gcaacgatga cattgatcaa tacatttacg ctggaagtat tgtccaagga
    13261 atcaatgagt atttagcttt gtatactgga cataatcgca aagctaagtt ggatggaaaa
    13321 acttcagaag tgttaatgat tgcaagttct aaagatttaa ttcattggaa aaaggatggc
    13381 gaagcagcaa aacttgttcc tcaagaagga tatgataaga atgattggcg cgatcctttt
    13441 gcaatttttg atcaagatca tggaaaatac gttttgatac taggagctag aaaaccagca
    13501 agtaagaaaa agccgacggg gagattggtc tattttgaat ccgaagattt aaaagattgg
    13561 aatttcaaag gagactactg ggctaataat gaatttaaca tgattgaaat gcctgatata
    13621 tttgaaattg atggaacgtg gtatttactt ttttcagagt attgtgaaga taaaaagact
    13681 aaatatcgag tcggagcagg attattttca gattggaaat caccagtaga cgaagctttc
    13741 gacggaaaag cctattatgc agcaagaaca gtcggaacta atgaaactgg ccgttttcta
    13801 actggctggg tggcaacaaa agaaggggag aacgatcaaa ataactggga ttggggcggt
    13861 gcacttgttc cacatcaaat tattcaaaag acagacaaaa ctcttggtgt taaactgcct
    13921 gaaaaaataa ttgagaattt taaagaaatc actccatttt caagttttaa gttagagaaa
    13981 gaatttggaa aaaaagaaag agtaattgtt cctaaaatcg aagattattt tctatttgaa
    14041 acaactttga ctattacatc gttaacaaag gagtttgctt taaaattatt tgtagatagt
    14101 gatactcaag aaggatacga atttcgtatt tcattgatta ataatcagct tactttcgga
    14161 aaaactccga actatccttg gcctcaaatg tttgataagg ggctgcaacg accactattt
    14221 ttggaaatag gcaaagaata tgaactaaag ttaattgttg atgatacgat agctgtttta
    14281 tatattgata atgttgcttt aagtactaga atgtatgata aagtcggaac agcattaagt
    14341 atgactgtta cggagggagc aatcaaggta gataaactag actattcaat aaattatgaa
    14401 tgtggatttt aaaacaattg aagggatgat gtaagtgaaa ttaaatggtt tacaacatat
    14461 tggaattcca acaaaagatt ttggagcttc aaaaatcttt tatgaatcct tagggtttag
    14521 tctgattaat gaagaaatga atgtgggaag taaagtcggc tttttcgagt tgaatggaac
    14581 gataatagaa atatgggaag ataaaactac tgagtgtaga ggagccattg atcatctagc
    14641 attagatacg gatgacatag atgaggtatt caattcaatt caagatttag gatatgaatt
    14701 attagataaa aatattatgg aattaccgtt ctgggaaaat ggaatccgtt actttaattt
    14761 ctacggacca aatcatgaga ttatagagat aagccaaatg aagaaataaa tagataacga
    14821 agtgctctaa gttggtatga acgaaaagta cttcattttt tggtatcagc atttcaatag
    14881 tttaatgaga atttactatt tcaaattaat tcaaagtgac agtatttttt acttatttgg
    14941 tttcaaattt tgtaatcctt cctatttatt ggaatacact ccacattttg tatacaagtc
    15001 atatcatgtt ttagcaaagc tcttaccaag caaagttata atgatgtttt ccaaagtttg
    15061 atctttcgat ggaggctaac acatattttt tgcaggacag ccaaaattag atgtatagaa
    15121 tagagtctgt gaggcgtata gacagtttat tgtctgtatg ccttttttta ttttgaaaaa
    15181 tttttagtat ttttcgaaaa atgctcactc ctagagtaag cgccccatag aacagtacct
    15241 tactaacaaa tgcacctcaa gatatacttg agctatcaag aagtctggag gttttc
//
LOCUS       DACCCS010000065        14995 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.066, whole genome shotgun sequence.
ACCESSION   DACCCS010000065 DACCCS010000000
VERSION     DACCCS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.066"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IVK82_002226"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IVK82_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP6393271.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IVK82_002227"
     CDS             complement(322..891)
                     /locus_tag="IVK82_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP6393272.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IVK82_002228"
     CDS             1077..1979
                     /locus_tag="IVK82_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6393273.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IVK82_002229"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IVK82_002229"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP6393274.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IVK82_002230"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IVK82_002230"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP6393275.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IVK82_002231"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IVK82_002231"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP6393276.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IVK82_002232"
     CDS             8547..8732
                     /locus_tag="IVK82_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP6393277.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IVK82_002233"
     CDS             complement(8794..9990)
                     /locus_tag="IVK82_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6393278.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IVK82_002234"
     CDS             complement(10160..11596)
                     /locus_tag="IVK82_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP6393279.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IVK82_002235"
     CDS             11889..12698
                     /locus_tag="IVK82_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6393280.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..14881)
                     /locus_tag="IVK82_002236"
     CDS             complement(12884..14881)
                     /locus_tag="IVK82_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP6393281.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4378 a         3179 c         2659 g         4779 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatcacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaa
//
LOCUS       DACCCS010000066        14840 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.067, whole genome shotgun sequence.
ACCESSION   DACCCS010000066 DACCCS010000000
VERSION     DACCCS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.067"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            418..1032
                     /locus_tag="IVK82_002237"
     CDS             418..1032
                     /locus_tag="IVK82_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6393282.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            1805..3544
                     /gene="yfcC"
                     /locus_tag="IVK82_002238"
     CDS             1805..3544
                     /gene="yfcC"
                     /locus_tag="IVK82_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP6393283.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            3701..4885
                     /gene="iadA"
                     /locus_tag="IVK82_002239"
     CDS             3701..4885
                     /gene="iadA"
                     /locus_tag="IVK82_002239"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP6393284.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            4996..5199
                     /locus_tag="IVK82_002240"
     CDS             4996..5199
                     /locus_tag="IVK82_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP6393285.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5300..5962)
                     /locus_tag="IVK82_002241"
     CDS             complement(5300..5962)
                     /locus_tag="IVK82_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6393286.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(5975..7477)
                     /locus_tag="IVK82_002242"
     CDS             complement(5975..7477)
                     /locus_tag="IVK82_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6393287.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8025..9704
                     /locus_tag="IVK82_002243"
     CDS             8025..9704
                     /locus_tag="IVK82_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP6393288.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            9831..10310
                     /locus_tag="IVK82_002244"
     CDS             9831..10310
                     /locus_tag="IVK82_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP6393289.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            10529..11209
                     /locus_tag="IVK82_002245"
     CDS             10529..11209
                     /locus_tag="IVK82_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP6393290.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11217..12548
                     /locus_tag="IVK82_002246"
     CDS             11217..12548
                     /locus_tag="IVK82_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6393291.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            12563..13525
                     /locus_tag="IVK82_002247"
     CDS             12563..13525
                     /locus_tag="IVK82_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP6393292.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            13636..13974
                     /locus_tag="IVK82_002248"
     CDS             13636..13974
                     /locus_tag="IVK82_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP6393293.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            14053..14760
                     /locus_tag="IVK82_002249"
     CDS             14053..14760
                     /locus_tag="IVK82_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP6393294.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4762 a         2578 c         3192 g         4308 t
ORIGIN      
        1 tttttttgta ttttcttgct tgcacttgga taagagatta cttacaataa attcagatag
       61 aaacagctga ataaaaaaag gctccctgac ccttagttgg cgctaagaat cagggaatga
      121 ccagtcacac acatacaccc tgaccgatca ttttgccata gtcaatgcag tgcatcgacc
      181 taatttattg tacctaatct agggtgaaaa ttctacccct atgtagatat tttccatggt
      241 ctgcacgttg ttttatttgc tatgtcaagg tattgggcgt attcgttcaa tgcctttttt
      301 gtgtttgaaa cagcaaagac cacatgtgtt actgtctaac tactgttttt ttatgtactt
      361 ccttagatta ggcagctgtt tctattcatg tcaagtatag ctaggcggga gagataaatg
      421 agtttatgtt attttgacta ttatcaccaa ttggaagaga aattaaggat agaagagccg
      481 ctgatgcttc aagctggcac ttggatcgcg aaacaaatta atcaaggtgg tagattgcag
      541 atatatgcta gccgcacact aagtggtgta gcatttgaat tttgggatca aaccccggat
      601 cttgtaccaa gtatattgat tgaaaatcca gccggaggtg tctatgaaac gctagaaggt
      661 gcaggacagg caattattga tcagctttct gtcaaatcag aagacatttt tcttcttctt
      721 tcgaatgaag gaagaaaccc ggcaatcatc gaactagctc agtggatcaa agaaaatggt
      781 catccattaa tcattgttac tggatttgat ttgtcacgaa gtatcaaatc gcaacatagc
      841 agtggtttgc gattatttga atttgctgat ttggttttgg acaaccacgc caatttacat
      901 gatgcagtct tgactttgcc aaatctagat ccagcgatct gcggaacagc ctctcttgca
      961 acgatctttt tgctgcagca agttttgtat tttacagcaa aacagctagt ccaggacaca
     1021 agtggaaaat agttatccac atgttgacaa agttccttaa aaaaataatg ttccacataa
     1081 aaatggagcg tttgaaaaaa agaaaacgat tattaatgag gattaacaac gactactttt
     1141 tttgttttag ttattttaag atggatcatc tttgatttta agcttgtttt gtttaaagtt
     1201 tgttattttt ataagttgtt tcacagttat gagaaatgag ttataagaat atgtagtgtc
     1261 agaaaaataa aaagtagtgg aaaaagagac aagaaagaat ccatatggat tctttcttgt
     1321 ctcttaatac acgttgatga aagagtcttc cccaaaactc tttacaaagt aattatagta
     1381 catttgttat cattattgca atgaagttta cctgatataa caaaattgtc caacaggata
     1441 cctttttcta atagaaataa aatacacaaa aattgaagga tagataatgg atatttataa
     1501 gtgtttatta ttttttttgc aatgaaattc aatttttcaa tgattgtaaa atgaaaaaaa
     1561 caggacgaga ttttctctaa tctaaaaata aaaaaagaat tttaaggaaa aacagagaaa
     1621 taaaatactt aatataaatg gaaagagaaa aaaataatca tacataggag aaaaaaaaca
     1681 aaatagaaaa aagtgatttt ctaataaatt attcatgtgg aaatgtcatt tttactgtat
     1741 aattcatttg attatccatt ttttattcat caaaattact gaaaaaagaa aggaagaaat
     1801 aaatttgaag aaaacaaatt cttcaggaaa accgaaacgc gacttcacta aattatcgac
     1861 accccatacg tatgtcatta tttttggtgt agtcatcttt gcatggatac tgacttttgt
     1921 cgttccagca ggaaagttca gcacgcaaga tattgaatac aaagatgcaa atggcgaaac
     1981 aagtactcgt actgttttac gtcaagattc cttccgttat gcttacgagc tggataagtc
     2041 gtatgtgttt gatcaattag aagaactgca agatcatcca gcagaacgtg aaaaactgga
     2101 tgttccggaa aaaggattgg aaaaagtcat tgctgacgga gaaaaaaatc ttacgcagga
     2161 aaaactagat gaaatctcct tgacagatga tgtgctttac gatcagtacg gcgaaaatat
     2221 ctatgatact tctaaaaaat tgcataaaac agccaaaata tggggaacag atgattttgg
     2281 cggctttggt ttcttaaatt ttgtttttga aggactagta tctggagata agtatggatc
     2341 tgctgtaggg atcgctgcat tgatccttgt tgttggaggt gcttttggta ttattatgcg
     2401 taccggagcg atcgacgcag gtatctatgc gtttatcagt aaaactaagg gactggagcg
     2461 tttagcttta ccgctattat tctttgcttt ttcattcggt ggcgctacat tcgggatggc
     2521 agaagaggta attccattct caatggtgat ggttccattt gtcattgcgt taggatatga
     2581 ctcgatcgta gcagtaaccg tgacttatgt cgcatctcaa gtcggaaatg cgacctcttg
     2641 gatgagcccg tttagcgtag cggtggctca agggattgct ggaatccccg tgttgtctgg
     2701 tgcgacattc cgattgatta tgtgggtagt tgttactgca ttagccgctg gttatatgat
     2761 gatatatgca gaaaaaatcc gtaaaaaacc agaaagttcg ttgacttaca aatcagatga
     2821 ttatttccgt tctcatatca aaaagacatc tgatgaaaat aaaaaattca tgcttgggca
     2881 taaattgatc ttattagaga tgttagttgt ccttgtctgg atcgtttggg gcgtgactca
     2941 aaaaggctat tacattccag aaatcgcttc tcaattcttt gtgatgggat tagttgctgg
     3001 aatcattgct gttcttttca aattagatgg aatgaaagtc aatgacatcg cttcttcttt
     3061 ccaatctggt gcagctgatt tagctggtac agcaatcgta gtaggtatgg ccaaagggat
     3121 tcttcttgtc ttgggaggat ctgatgcatc tgttccttca gctttgaata cgattttgca
     3181 tgggatcggg acggcgttga ccggtgttcc agctgtcatt ggtgcttggg cgatgtacat
     3241 cttccaaagt ttgttcaact tggttgttac atcaaattca ggacaagcag ctttgactat
     3301 gccaatcatg gctccactgg cagatttagt aggtgtatcc agacaagtag cagttcttgc
     3361 ttatcagctt ggttcaggtt ttatggacgc ttttacaccc gtatcagcca gcttgattgg
     3421 tgtgctaggt gttgcacgga tcgaatgggc aaaatgggca agattccaaa tcaagatgca
     3481 aggattcctg tttgttttag gaactatttt catcatgatt gccattgcaa taggtctgca
     3541 gtagtactgg agacctgtaa ataaaaaagt aatccaaaca gtagataagg actccaagaa
     3601 ccgtcatttt acggtaaaat caaaggagta aagatcctat cgtttggatt attttttaag
     3661 atcctatcat cagttatact taaagaaaga ggaaaaatgt atgaaaatca ttcgacaaat
     3721 tgaagtgttt gcaccagatt acttaggaaa aatggatgta ctgattgccg gtgggaagat
     3781 tttggctgta gaagaacagc tagaaggcgg ctacgaagga gtggaagtag aagaactttc
     3841 aggtgaggga aaagtactaa ccccgggttt cattgactgt catttccata ttttaggtgg
     3901 aggaggagaa ggcggttatc aaaatcgaac gccagaagtc acattgagcc aattgactac
     3961 agctggtgta acgacagttg tcggttgttt gggaacagac ggagaaggta gagatatgac
     4021 tgcgttgatc agcaaagcga aaggtctgga agcagaagga atctccactt atgtatacga
     4081 aggttcgtac cgattgccgg taaaaacagt aacagattct atcatcaaag actttttgac
     4141 aattgataaa attatcggaa tcggagaaat cgcagtatcc gatcaccgtt cttctcagcc
     4201 aagttttgaa gagttcgctc gtgcagtagc agatgctcgt gtaggaggca tgttatccgg
     4261 aaaagctggg atcatcaatg tccacttagg cggaggaaaa agaaagctag agctattgac
     4321 acgaatcgta gaagaaacag aaattccgat cacacaattt ttgcctactc atgcaaatcg
     4381 cacaccagaa ttatttgaag cttgcgtggc atttgccaaa cgtggtggca cgattgactt
     4441 cactgctagc gaagatcctg atttttggga gaagacagat ggtgaagttc gcttcagcaa
     4501 agcactgaaa cgattgatcg aggaagatgt ttctttagat aatttcacga tgacctcaga
     4561 tggacaagga agcttgcctt attttgatga aaacaatcat ttcctagggt taggagtagg
     4621 aagtgccaag gcattgctag tagggatcaa agaagctgtg caaaaagaaa gtatcccttt
     4681 ggaaatcgcc ttacgagcta taaccagtaa tccagcacgt atattgaaat tggataaaaa
     4741 aggaaaaatc gaaattggtg ctgatgcaga tctgtgtatc ttggataaag aaacgcttga
     4801 tattgataca gttatcgcta aaggagaaat aatggtccaa gaaaaagaag tcaaagtatg
     4861 ggggactttc gaaaaaagtt tttaatcaac agctctaact atctttttta gaaaacgagc
     4921 gttctgtttg tctgtatctg ttgttgatgt taccctgaag gtagatcaat aaacaaacta
     4981 agaggaggaa gcacaatggc agatttaaaa ggacgttcta aagatatgaa agataaagta
     5041 gtcggtgaag gaaaagaagc atacggcaaa gcaacagacg acaagagcaa agaaacagaa
     5101 gggaaagcac aatcactagg tgctgacatc aaagaaaaag ctcgaaattt aggcgacgaa
     5161 gtcaaagaca agttttctcg aaaagacgaa aaaaaataaa aaccacttat tactaattga
     5221 atcaagcaaa taaaaaatcc gaaccatatc tgcgagacag atatggttcg gattttttat
     5281 ttgatcatta tcctgttttt taaacttcga ttttttgaaa ttttttattt tttagttcaa
     5341 agacttcatc acagcttttt gctacttcaa tctcatgagt cacgatgata atacagcgat
     5401 tttgttcatg ggcgatttct tggaaaagtt tgacgatatc ttgtgaagtt gtttcatcta
     5461 gatttccagt aggctcatcc gctacgatta gttgatgttc acagcagata gcacgaacga
     5521 tagcgactcg ctgctgctga cctcccgaaa gtttggtcac ctgttttttt gctaatattt
     5581 catcaattcc tacacgagcc agtcgatcta aagcaacttg tcgataattc tcttgcttgg
     5641 tcttagtgat ttccattgct gctatgacgt tgtctagagc cgataaataa ggaagcaagt
     5701 tgtaagcttg gaaaataatc gagacatcat ttttacgata acttcttaac ccgattttgt
     5761 tcagtgaacg atcttgatag tatacctttc cttcttttgg tgcatctagt ccagaaataa
     5821 gcgaaagaaa agtcgttttt ccagaaccac ttgctcctaa aatagcgtac atttttccag
     5881 cttcgaaatt cagttctaca tctttataaa gcgcatcttg cggatcttga taccaaaatc
     5941 ccaattgttc tgtccgtaac atcaaattcc tcctttagtt taataggatt tttcttgggt
     6001 ttaatcttaa tatccctata gaagctaaaa agacagaaaa cagactgatt ccaaatgcta
     6061 ctccggctaa tagtcccaat tgtgcaggtt tgacattgat ttccaattcg ctgacttgat
     6121 cagaaacagc aaatggatta ttcgtggttc cttgttgacc atcgcgttga ccgccttgtt
     6181 cttgtctgtt acctggcatt tgtccaccgc caggagcttg ctggttttgt gtcgtggttt
     6241 ctgtctgttg gtcaagtagc tgctgtccca cggcattcgc gacgacgttt ccgctaagcg
     6301 aagcaactcc taatgcaaga atcatacaaa tagccacttc ggtaaacaat tggagaatga
     6361 ttttacttct ggattctcct aaagatagca agacgcctaa ctcatgccga cgctcccgaa
     6421 tggtaatcat aatgatcaga gtaagtatga taataccggc tacagccact agtaaaacaa
     6481 catttttaga aaaacttgcc acgttgttta aaggttccaa cattgactga tacatggaat
     6541 catttgattg taagctaaat gtatctgtgt cgatcagctt ctcagcttct ttgagaaact
     6601 catccatttt atctggatca gatagagtgt aaatagcaga atctatcgtg ttatcgtcag
     6661 atgtcccatt tagttcattt gcaaaagtgt aagaagtgta aagtgtattc gacggattca
     6721 tgaagttgaa tttcataccc atactgccgc ttgtttcgct ggattcataa attcctttga
     6781 tcgtcatttc ataagtggta tcttcatctt cagtactcgt gattgtgaaa gtatctccga
     6841 ctgccaggtc atttgtttct gctagagtag aatcaatgac tacattgttt gtgcctttgt
     6901 cgctgtcatc tattccttca ccatccgtga tttttgctgt tccatcagaa aaattactat
     6961 tttgtgcagt tgctgacaca ccactgattt gaaagtctgc ttgagtcatt tgcggattcc
     7021 cgcccattcc tccagcaggt tcaccgcctg catcagtagt ttctgaactg tcttcacttg
     7081 aatcagaact agtgatggca gttattccat cttttgccat agctgaggcg gaactttcaa
     7141 aggaataact ctttacatta tctaattcag ctattttttc cgcgtccgtt aaggaaactg
     7201 gtgtaagaga aaaactgcct ggatctggac gttcttcagt ggtagaaccg cttgaagaag
     7261 tttgttcctg ttttttaaat gaagcttctc gattggcact taaagtcact gttgctccaa
     7321 cttctttttg tgcttgattc gctgcttctt ctgcagcgct tcgaatcgtg aggccagcta
     7381 gcacaaaaat aagaatggct gtgaacacgg caaacaataa caatgaacgt ccccatttta
     7441 cttttgtact tttccatgca cgtttcataa aattcatatt ttgcatccct cctagtagat
     7501 gcatttgata atagacaacc tatcttaaag ctagcttaaa tcttatgctg tttgcacacc
     7561 atcaacatga ggttctgtat atataaaaag tcactatagt tattgcaaaa aatgtggtaa
     7621 aatgaaaaaa gtatgtttca aaccgacgaa gaagaaatga aagaggaata gcaaaagaaa
     7681 tcctagatgc tacagaggga tttttacgtg catagttgtt ctaacaattg ctgatactag
     7741 cgatttagtt tatttgttgt ataataatgg ggaacagctg aatacttata taagtattct
     7801 ggctatagcg tttcgcaaaa gatccatttt gttaatgaag tatatccaaa ataaaatgcc
     7861 catccaactg tcagaacgcg gatgtgctgg taattttcct gctgattctc cataagttga
     7921 gaaatagtga ttttgcatat gcttcattgg aatggctcat atatttagat aaagaagaaa
     7981 atcgacatct ttaaacttgg atgtcataag aagaggtgaa gtttgtgagt accattaaaa
     8041 ttatcccatt aggcggcgtt cgtgaaaatg gaaaaaacat gtatgtcgca gaagtgggcg
     8101 aggatatttt tattttagat tgtgggctaa aatatcctga aaatgagttg ttggggatcg
     8161 acgtcgtaat ccctgatttt acgtatttag aagaaaacat tgatcgtgta gctggtgtct
     8221 ttttaacaca tggacatgca gatgcgatcg gggcacttcc ttacttgttg tcaaaaattt
     8281 ctgtaccagt attcgggaca gagttgacga tcgagttagc aaaattgaat gtcagtcgta
     8341 atgaagcagc caaaggattt aaggatttcc atgtggtaga tgagcataca gaaatagact
     8401 ttggtgaaac agtagtgagc tttttccgta ccactcatac gataccagat tcagtgggga
     8461 tcaatctgaa aacaaaagaa ggtagtatcg tttatacggg agattttaaa tttgaccaaa
     8521 cggctatccc aatgtatcaa acagattatg cacgattggc ggaaatcggc aaagaaggag
     8581 tcttagcctt actaagtgaa tcggcaaatg cagaaaatcc tcagcctgta gcttctgaag
     8641 cccaaatagc agatgaagta tttgatacga tcaaatattg ggaagggcgt atcatcgtcg
     8701 cttgtgtagc aagtaatttg caacgagtgc aacaagtatt ggatgcagca gaaaaagcac
     8761 aacgaaaagt tgtcttgact ggtcaagatt tcgaacgaat tattcgcaca gcgatgcgtt
     8821 tagaaaaact tcaattacca agtgaagatc tgcttgttac gttgaaagac atgaaaaact
     8881 atgatccaga agaactgatc attttagaaa ctggccgaat gggtgaaccc atcaagtctc
     8941 tgcaaaaaat ggcaaacggt acacaccgta atgtcaagat tgaagaagga gatttggttt
     9001 atatcactac tacgccaagc atcgcgatgg aaaccatcgt ggcgaaaacg gaagatatca
     9061 tttatcgagc tggtggaaca gtgaagttga tctctgagaa tatgcgcgtt tctggccatg
     9121 ccaatcctaa tgacttgcaa ttaatgatca atttgatgaa accaacatat tttgtgccag
     9181 ttcaaggaga gtatcgtgaa ttagctgctc atgcagattt ggctcatgca gtagggatgc
     9241 catacaaaaa tatctatatt actggacgcg gagacgtgct ggaatataag aacaagcgta
     9301 tgagtgttgc aggaactaca agtgcagaaa atgtaatgat tgatggatta ggcataggtg
     9361 atatcggaaa cattgtttta cgtgatcgaa aaatcttgtc agaagacggg attttcgtag
     9421 cggttgtcac tatcagtcgg cgagaaaaga aaatcatttc aaaacctcaa attacttcac
     9481 ggggattcgt ttacgtaaaa gctagccgtg atctgatgaa agaaagcagc aatatgattg
     9541 aaacaatcgt tgagaaacat ttagaaagca atgattttga gtggagcaaa ttaaagcaag
     9601 acatccgaga tcaattaggc cgttacttat ttgaacaaac aaaacgccgc ccagtgatct
     9661 tgccagtcat tatggaagct actcaacgtc gaaataaaaa ataaacaaaa agagtaagat
     9721 gaacctctct atagagtgct cgtcttgctc tttttttgcg aaaaacaaat aatttttgaa
     9781 tacagcatcc ctcgctttat aataaacgat agaataaagg gagtgaatca atgatgagtc
     9841 aaacaaaagt cgaacgttat ttagaagaac aaaagatcag ctatcaacca cttgatttta
     9901 gtcatgtggt agaagaatct ttcacagaag aactacaaaa aagagggatt gatccagctt
     9961 tgatatgtaa aactctcgta gtgaaaggaa acaaaactgg ggtgatcatc gcattgattc
    10021 ctttgaacga tcacttagat tacaaaaaaa cgagacaagt gacgaaagat cgtaaagttg
    10081 gttttcctgg aatggatttt gtattaatgc atacaggata tccccacgga gcaaacaccc
    10141 ctattggaat aaaactcgca catcctgatt attgtttttt agcagacagt tctttaaaag
    10201 aaaaagaaga agtaattgtg tcaagcggtg aaatcgggag aagccttcgg ataaaagtag
    10261 ccgatttaga aaagatcgtc catcctattt acgctgattt aattagttaa gggacgagaa
    10321 gagaagctaa gcagagcaga gacctcaaac ttgagattat atctgcttgg tacttggtgc
    10381 atcctgtatt atctgttaca attgctttgt acatactgta ctatcaatcc aatgaaaata
    10441 tatttagcag ctatattttt tcattgatgt gcaagtgatg aaatcgtttt taataaaaaa
    10501 gatacagaga aaacgcgagg tgtaaaatat gaaaattacg tatcatggac attcctgttt
    10561 aacaattgaa atggaaaatg gccaacgcgt gatgattgac ccgtttatta caggaaatcc
    10621 attgacagac ttaactgcag aagaagtaaa gacagattgg ttgctgatta ctcatggtca
    10681 tagtgaccac ataggagata tgattcctat tgcaaaagaa aatcaagcca caattatcag
    10741 tattgttgag attgctgatt atgcgaacag cagaggagta gattcattcg gtatgaatat
    10801 cggtggaaaa catgcgtttc cattcggcgt ggtaaaattc gtacatgcac agcatagttc
    10861 aagctatgaa gtagacggta tcgttcagta tatgggcgag cctagcggaa ttattattca
    10921 agcagaagga aaaacgattt atcatgcagg ggatactgct tatttcagtg atctaggatt
    10981 gcttgcagaa gaattcgata ttgatgtcgc tttcttgccg attggcgata actatacgat
    11041 ggggccagaa gatgcagcaa aggctgcgca aacaattcgt gccaagaaag tagttccaat
    11101 ccattacaat actttccctg tcattgaaca agatcccgaa tcttttgtag agttactgga
    11161 agatagtgaa ggaaaaataa tgagcgtctc ggaaacgctg gaagtgtagg cagaaaatgg
    11221 cgacaaaaca tgatttgatt ttagaatata ttgaaagttt acctgttggc aatcgaattt
    11281 ctgtaagaag catcgcaaaa gaattgaatg tcagtgaagg aacagcgtac cgtgcaatca
    11341 aagatgcaga aaatgtcgga ctagtttcaa caattcaacg agtagggacc atccgtatcg
    11401 aacgcaaatt aaaaaaacat attgaacgtt tgacatttgg cgaggtcgtc aggatcattg
    11461 aaggggacgt tttaggcggg gcggcaggat tagacaaggt cttgaataaa ttcgtcatcg
    11521 gcgcgatgac taaacatgcc atggaacgct atattacgcc aggatcacta atgatcgtcg
    11581 gcaaccgggc ggaagtccaa aaattagcat tggaagatgg cgccgcagta ttgatcacag
    11641 gaggattcaa tacgactgct gagattgcaa agctagctga tgatcttgaa atgccgatct
    11701 tacgcacctc ttatgataca tttacagtcg caaccatgat caaccgagcg ttaagcgacc
    11761 aattgatcaa aaaagatatc atgcttgtca gtgatatcta tatgccagct gaaaagactc
    11821 attatttgca gcagacagat acgatttctg attatcagcg tttgtcagaa gaaacgcagc
    11881 attcgcgttt tccagtcgtc aaccgccatc atcgtttgat ggggattgtt acggcaaaag
    11941 atgttttagg gaaatcgcca aaccagatca tcgatcgggt gatgacgaaa gaaccaatca
    12001 gtgtaaagaa aacaatgagt attgcctctg ttagccatca aatgatctgg gatgggctag
    12061 aagtcatgcc ggtcgtttcc gatgacttga cattggaagg cttgattacc cggcaagatg
    12121 taatgaaagc gatgcagctt gtccagcgac aaccgcagat tgcagatacg atttcggatc
    12181 agatatctgg aaatatccat acgattgata ctgaccgtga aggaaatcgt ttggaaaacc
    12241 ctaagttcaa attcgtagta gcaccacaaa tggtcaatgg cgttggaacc atttcttttg
    12301 gcgtacttag cgaaatcatt tctgatgtcg cacaaaaaac aatggtgatg aaccaaaaac
    12361 gaaatatctt gattgaacaa gtgaacctac attatttgcg gctgatccaa ttggaaagcg
    12421 aattagacat tcgtccacgt gtattggaaa tcggcagacg ttccgctaaa ctggatatcg
    12481 aagtgtttat tgaaaacacc atcgtttcca aagcaatcgt tgtttgtcaa gtaatggaac
    12541 gttcttagaa agcaggagtg aaatgacagt acagcaagaa atagtagata caataaaaaa
    12601 atacgataaa atcattatcc acaggcattt gcgccctgat cctgatgcat taggatcaca
    12661 agtaggatta gccgaactat tacgggaaag tttcccagaa aaagaaatac gtcaagtagg
    12721 tgaaacgatt gaagggcttc gctttttagc agaaatgcaa gaagttgaag atgtcttcta
    12781 ccaaggagcg ctagtcatcg tgactgatac agctaattca cccagaatca gtgatcaaag
    12841 atatcagttg gcagaaaaac tgatcaaaat cgaccatcat cccaatgatg acccttatgg
    12901 agatcttgtg tgggtaaata caaaagcaag tagctgtagt gagatgattg cagaatttgc
    12961 tttgatgttt cctgaatctt taaagatgaa cgttaaggct gcacggttac tttatgcagg
    13021 aatcgtcgga gatacagggc gctttttgta tccctccacg acgtcccgta cattagagat
    13081 tgcttcgata ttgagaaatt atccatttga tgcaagtgct ttgaacagag agattgaaca
    13141 aatgccgatg aaagtcgcta aactttccgg ttatctttat caaaatatcc aggtggatga
    13201 aaatggtgca ggaagaataa tccttccgca agaaattctt gataaatatg gaatcgatga
    13261 ttcagagaca gcttcgatcg tttcacttcc tggagtgatt gacgaagtgc tggcttgggg
    13321 gatttttgtt cagcagcctt cagggtatta ccgcgtacgt ttacgttcta aggggccgat
    13381 catcaatgaa ttggctaaac gccatcatgg aggaggccat ccattagcaa gtggtgcgaa
    13441 tgcaaaagat ttagcagaag tagaggaaat ctataaagag atccaagata tatgccaatc
    13501 gtataagagc agaaaaacag aatgatactt cgttcgaaaa cgtgcctttt gtagtaaaaa
    13561 tcacaaatgt atcttttcaa gatcttcttt ttccgttaca atagtgctca ctaaagattt
    13621 taaaggagaa agaagatgac attaccaaat tgcccagaat gtggctcaga gtacacatat
    13681 gaagatcgag gcttgttgat ttgcccagaa tgcgggaacg aatggaacaa agaagaacaa
    13741 gaaactgtca tagaagacgg attagtggta ttagatgcaa atggaaaccg tctgttagag
    13801 ggcgatagcg tgacagtgat taaagacttg aaagtaaaag gtgcgtcagg tgccattaaa
    13861 caaggaacaa aagtcaagaa catccgttta gtagaaggcg atcataatat cgattgcaag
    13921 gtagaaggat ttggaccgat gaaactgaaa tcggaatttg tgaaaaagaa ttaaagaata
    13981 ttcaaggtct tttcacattc ttttcctact actaatgata aaataaagta aacgaaaagg
    14041 agtgaaacga aaatgtatgg ttatggaatg tatggttttt tagatccgac atttcttttg
    14101 gtcattcttg gattagttat ttccggaatt gcgtcagcgt acgtcaatag tactttccga
    14161 aaatacgatc aagtgagaag caaaaacaat gtgactggta cacaagcagc acaatatata
    14221 ttacaaagtc aacagataaa taatgtcggt gttcaacaaa ttgccggaga tctcacagat
    14281 aactacaatt caggtaataa gatgttgagt ttgtctcaag caactgcaca gtctacttca
    14341 gttgctgcga taggtgtagc agctcatgaa tgcggacatg cggtacaaga tgcttcgggt
    14401 tatgtcccat tgaagattcg tgcagcactt gttccagcag ctaactttgg ttcgatgatt
    14461 tcgttcccct tgattatggt aggtgtattg cttagctgga actctacgtt aatcaatatc
    14521 gggattttcg ctttttcttt agcattactt ttccaattgg tgacattacc agtcgaattt
    14581 aacgcgtctc ggcgagcaat ccaaattttg agtgaaggcg gactactgac ggaagaagaa
    14641 gtaccgatgg caagacacgt attatttgca gcagcactga cttatgttgc agcagcgctt
    14701 tcgacattcc tgcagctgtt gcgcttgatc ttattgttcg gcggcaaccg aagagattaa
    14761 aataaagaag ttgtgaaatc agcgctttga tctgagctaa taccgagagt tagctgattg
    14821 aataccattt atttggaata
//
LOCUS       DACCCS010000067        14738 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.068, whole genome shotgun sequence.
ACCESSION   DACCCS010000067 DACCCS010000000
VERSION     DACCCS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.068"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(547..909)
                     /locus_tag="IVK82_002250"
     CDS             complement(547..909)
                     /locus_tag="IVK82_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393295.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(983..1363)
                     /locus_tag="IVK82_002251"
     CDS             complement(983..1363)
                     /locus_tag="IVK82_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP6393296.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1445..2620
                     /locus_tag="IVK82_002252"
     CDS             1445..2620
                     /locus_tag="IVK82_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6393297.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2697..3281)
                     /locus_tag="IVK82_002253"
     CDS             complement(2697..3281)
                     /locus_tag="IVK82_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6393298.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3482..4480
                     /locus_tag="IVK82_002254"
     CDS             3482..4480
                     /locus_tag="IVK82_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6393299.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4592..5101
                     /locus_tag="IVK82_002255"
     CDS             4592..5101
                     /locus_tag="IVK82_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP6393300.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5131..5790)
                     /locus_tag="IVK82_002256"
     CDS             complement(5131..5790)
                     /locus_tag="IVK82_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP6393301.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5792..6556)
                     /locus_tag="IVK82_002257"
     CDS             complement(5792..6556)
                     /locus_tag="IVK82_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP6393302.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6564..7241)
                     /locus_tag="IVK82_002258"
     CDS             complement(6564..7241)
                     /locus_tag="IVK82_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP6393303.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7278..8663)
                     /locus_tag="IVK82_002259"
     CDS             complement(7278..8663)
                     /locus_tag="IVK82_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP6393304.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8873..8957)
                     /gene="ffs"
                     /locus_tag="IVK82_002260"
     ncRNA           complement(8873..8957)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVK82_002260"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8980..9495)
                     /gene="tadA"
                     /locus_tag="IVK82_002261"
     CDS             complement(8980..9495)
                     /gene="tadA"
                     /locus_tag="IVK82_002261"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP6393305.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9602..10459
                     /locus_tag="IVK82_002262"
     CDS             9602..10459
                     /locus_tag="IVK82_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP6393306.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10540..10875)
                     /locus_tag="IVK82_002263"
     CDS             complement(10540..10875)
                     /locus_tag="IVK82_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393307.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10942..12312)
                     /locus_tag="IVK82_002264"
     CDS             complement(10942..12312)
                     /locus_tag="IVK82_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP6393308.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12390..12926)
                     /locus_tag="IVK82_002265"
     CDS             complement(12390..12926)
                     /locus_tag="IVK82_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6393309.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13235..14716
                     /locus_tag="IVK82_002266"
     CDS             13235..14716
                     /locus_tag="IVK82_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6393310.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4526 a         2927 c         2393 g         4892 t
ORIGIN      
        1 gagtgtttcc cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataacta ttttttaaat tttttagaaa tgtgatataa catccacttc
      301 agaatcaacg ctgttaaatt tatcatgtat ttcttccttg gtcaactata aattaagcat
      361 tactttgtga aatgattttt cgatcaatta ttattattcg ttatacaacc gttttcaaca
      421 tattttgaaa ataaaatgca ctaaaaaact atctttagca gaacatttca ttgtattttt
      481 tggaccaaac aataaaaaaa gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc
      541 tgtattttag aagaaaggca ctccctctac ttcgtaagtt ccaagaacag agcgcatttg
      601 acctggttct tcccaaatat atccttcgcc taattcttct ctttgattct tttgttcagt
      661 ttcaaagaca aacaacggaa cattttcctc tttaatatgt gtatttgtca attccatcaa
      721 actaatcttg ctaacatcga tatgaactat ttcttttaag aaattaagta tattcgctaa
      781 accagtcttt ccttcagaaa gttccgctaa cgcaaattcc aacttgtcat caattgaatg
      841 gaccaaaaat tttttagtac catcctccaa atgcaacata attgtaccag caactctttt
      901 gcttgccaaa atttccacct cgatcaactt gttctatggc ttacataaaa attctagcac
      961 attttcagaa aaaatacact ttttatttct tattttcttt ccgttctagt tcagataatg
     1021 cttcttctat ccaaataggt aactgctccg caatagtggt aaatccgatc ttacgatttt
     1081 tattcgtgaa ataccttttt ctgcgatatg gaacagctgg atttgttttt tctaacgttc
     1141 ttaaagataa actgattttt ttggaatatt catcaatgtc gatgatctga actgttactg
     1201 gctgtccgac ttttaacaat gaatggatat ttttcgtata accggattgt acttctgaaa
     1261 catgaataag tccttgcgtc tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc
     1321 ctgtcacttt tccttcaatt atttgtccaa ttttataagt catctacttc cctctcttcg
     1381 catttatcat tatactgtaa atagctcaaa aaaagaagcg acgaaaatga ctggaggtct
     1441 taagatgatc gaatttgata aacagatttc aagaaaagat acagaaagtg taaaatggga
     1501 cgcaattgct gagacatatc aaatggatga cttacttcct ttgtgggtag cagatatgga
     1561 cttcttgtct cccggtgggg tagctaaagc gtttagtgaa tacatcaaac atggaatctt
     1621 tggttatgcc actctgtctg ataaactata tcaggctatc attcattggg aacgccaaca
     1681 ccatgctgtg gagttgacga aagaaaatat cgtttttaca agtggtgtgt taaccagttt
     1741 agctgcagca gtacaaactt ttacaaaacc aggcgattct attttgattc atgacccagt
     1801 ttatcctcca ttttcctcta ttatagaaac aaatcaacgt cacatcgttc gaagctcttt
     1861 gctagaaaaa aataatcatt tcatcatgga cttgtctgac atggagaaaa aaatcgaaga
     1921 aaacaatgta aaagcaatga ttttatgcaa tcctcacaat cctggagggc gtgtttggag
     1981 caaagaagaa ttgaaacagt taagcgaatt gtgtttaaaa catcaagtga ttctgtttag
     2041 tgacgagatc catcaagatt taactttatt cgatcataca tttacttcca tgctaacgat
     2101 tgatcctaat ttagatgatc tactagttgc ttttacttcc gcaaccaaga ctttcaatct
     2161 agctgcaatc aaaaattcaa tggtatttat caaaaatcca gagatgaaag ctgattttga
     2221 aaaacattta gtgatgaacc aacagcacga aatcaacact tttggactga ttggaaccca
     2281 agcggcttat gagacgggtg gagaatggtt aagccaactg attccttatc tagaaaaaaa
     2341 tgttcaaaca gttaatcagt tttttgaaca gtatcttccg aaagtgagaa taatgaaccc
     2401 tgaaggaact tacttaatgt ggttagattt taccgcctat atttcagacg accaagaatt
     2461 ggagaacact cttgttcgga aaggaaaagt cgtattaaat cctggcatca cgtttggtcc
     2521 ttccggtcat gggcatatgc gcttgaatat tgcttgtcca gaagaaactt tactggaagg
     2581 acttaatcga atcaagtatg ctttagagac aatcgattaa atcatttatt caaaataaaa
     2641 ggacgagacc ttactaggtt tcgtcctttt tcataattac tccgtaccat accaaactat
     2701 tctgaaattt caattgtttc aatcactaca tcatgtacag gtcggtcttg tggaccacgt
     2761 tgtacatttg cgatttcatc tactgtgtcc atcccttcta gtacatgtcc aaagactgta
     2821 tgtcggaaat ctagccaagg agttccgcca cctttatacg cttcaatgat ttctgatggg
     2881 aatccagcac cttctagttg tccaagcata ttagctggca catttgtgtt atttacaata
     2941 aagaattggc ttccgtttgt gtttggacct gaatttgcca tagataaagc tccgcgaagg
     3001 ttgaacagtt cacgactgaa ttcatcttca aaactttcgc cataaatact ttcgccaccc
     3061 attcctgttc cagttggatc gccgccttgg atcatgaaat caggaataac gcggtgaaaa
     3121 atcacgccat cataataacc ttttttggaa agttcaatga aattttgaac cgtcttcggt
     3181 gctagttcag ggaataattg aacagtaatg tcaccacggt ttgttttgat aacagctttt
     3241 ggtccttttt cactttttaa atctagttgt gggaatgcca tattgtttcc tcctaatatt
     3301 tgcatacttc ataataataa taacataata cctaacctct cgtaaaaata ctagatttgt
     3361 tttcttttat ttttcatcac tagatacatt ctataactta ccgtttcgat ttcgaattca
     3421 aacatttttg agaagcattc tcaattaaat tctgctatag tgtgaaaagg gaggaatttt
     3481 tatggaaatc tatgacatta caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt
     3541 ttacgcagga atgcgccaag ctaaaacaaa aatcattgac agtctacctc aacttggtgg
     3601 tcaattagca actttgtatc ccgaaaaata tatttacgat attcctgggt atccagcaat
     3661 aaaagcaagt gaattaatcg atcaattaga aaaacaatta actactttta accatacatt
     3721 ccatttaaaa gaagaagttc tctcgcttac tcgtgaagac gaagttatcg agattactac
     3781 aaataaaggc atccattatt ccaaagcagt cattttagct ttaggaaatg gttcattcca
     3841 gccaagaaaa ttgaatttag ataatgctga atcatttgaa aaccacggat tggattattt
     3901 cgtgaatgat ttgatgaagt atgctggaaa aaaagtcgct attgctggag gtggtgattc
     3961 tgctatcgat tggacgttga tgttagagcc tattgcaagc gaagtttact tgattcatcg
     4021 tcgcccggaa tttcgtgcgc acgaacatag tgtatcacgc ttgaaatctt catctgtcaa
     4081 cttgcttact ccctatctca tcgatggatt atcaggcaat aatggggaac tgacagatat
     4141 ccggttaaaa aaagtgaaaa gtgacgagac gattgatttg atgattgatt ctctgatcgt
     4201 caattatgga ttctcttcta atttagaaca tctttcttct tggggacttg atagtaccag
     4261 aaatgccatt actgtaaaat cagatatgtc tacttctatt ccaggcgttt atgcagctgg
     4321 tgacatctgt acctatgaag gaaaagtgaa actgattgct actgggctcg gtgaagcacc
     4381 tactgcagtg aacaacgctt tacactatat taatccaaag gaacgaactc agcctggtca
     4441 cagcacaagc ttatacgata aaaatcttcg tcctagttga ttttttatat ctctttgcat
     4501 ttcaagttaa acccgaaagc agtttcttgc atttttttca tgttccgtta acatgaaata
     4561 gagaaagaaa aaataaaggg ggagcaataa tatggtcgtc aaaactgaga ctttagaaga
     4621 aaaggcaaga gaactattga aggaacgtgg cgtaactgta gaagatatcg cacaacttgt
     4681 catgttttta caaaaagact atatttctga tttgactttg gcggattgta ctgaaagtgt
     4741 caacagcgtg ctaacaaagc gtgaagtaca taacgccatt atcactggaa tccaattaga
     4801 tatattagca gaaaagaaaa aattgatgag tccgttgcaa catattatcg aagatgacga
     4861 aggcctttac ggtatcgatg agatcctagc cttgtcaatc gtgaatgttt acggtacgat
     4921 cggctttacg aattatggtt atatcgacaa ggtgaaacct ggtattttaa aagaactaaa
     4981 cagccacgat ggcgttcatg tccatacctt cttagatgat atcgtcggag cgatcgccgc
     5041 tgctgctgcc agtcgattag cacacaaaga accagaaaaa agaagtcgcc ttactcaata
     5101 attcaacaaa aaaagaaaga aaacgtctat ttagacgagt tctttctttt ttgtttgttt
     5161 caagctattt tttcctaaga aaagaaaaac agcaaaaaat aattctaata atacaaaaaa
     5221 taagataaag ctatgcatga aaaattcttc tggaagtaca ttgatgattg gatcagtaac
     5281 gggatcgaat aaccagtcat cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg
     5341 atcaaatcct atcatcatga aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa
     5401 cggacgaatc aaccgccaaa gccgatcatt cttttgcaaa tatttgataa acaaaatact
     5461 cgggatcaaa gtgatgaaaa acacagcata atccactaaa aacaaaattt tcacttcata
     5521 aaagtgatgg gctccgctag cagaaactgg gaaatctggc aagctgagga tggttttgaa
     5581 gggattattg agataattca ttaggtgatc aaaatttttc agtaacgtct cttgatctaa
     5641 agaagtataa tccaaaattt gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt
     5701 gatcgtcaca ccaactgcca gactgatcag tgtcagaaat aggctgaaca atccagcata
     5761 ttccatccac tgccatctcg aatttttcat attagaagct ccattcatcc agtgattgtt
     5821 tcacataggt agctgggata ggcaacccag gtacatcttc ttcttttgta aaacctgata
     5881 aaacgagcaa agtatctata ttattgcgta tcccagcttg gatatctgtt tcatagttat
     5941 ctccaaccat gattacttct tctttttgca gccctaatac ttcaacggct ttatccataa
     6001 tgatggcttc tggttttccg atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag
     6061 agatgactga ccctgctcca ggaagaaggc cgcgttcagt aggtatattc ttatctgggt
     6121 tcgttccaat gaaagtagca cctttttgaa tagcaagtgt tgctttaact actttttcat
     6181 aggtcagata attatctaag cctaccacta catagtcagg agcctcttct tcccatgtaa
     6241 atccggcagc taaaatcaag tcaatcaatc ctgcttctcc gataacatag acttttttcc
     6301 cttttccatc tgctttcata aaatcgattg ttgccaaagt agctgtgtag actgtctctg
     6361 gagctacatg aatgtcaaac tcatctgcta gacgattagc tactgtttct ggtgacttag
     6421 tcgtgttatt ggtaacaaac aagaaaggta attttctttt ttgcaattct tccacaaatc
     6481 gtttaccggc cggtatgggt tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat
     6541 atcctttata gtccattcat ccactactct tccctttttc tgatcgtaaa tgctgttttt
     6601 gttttttcta cttgtttagg ttgttcttgt ttttttactt tttcagaggt ttttgctgac
     6661 tctgtttttc gatttttaat aaccggtttt ttgcgattag atgcgactgg tccttttttc
     6721 tcatcgatat acgcacgatt atggtttttt ttcttttttc ggcgctgagt tgtcttttct
     6781 tttttaccgc cgattctctc gatgacaaaa tacgcacaac caaaattgca atattcgtaa
     6841 agatagtctt ccaaggaatc gattcgctga tctggatgcg cccgtcgatt atccgcttgg
     6901 aaaaatcctt ttaggcgtag ttgttcataa ccccaatctc ctacgatata atcatatcgg
     6961 gctagtacat cactgtaacg ttctccaagc ttttctgcat taaatccttc acgataatcg
     7021 gacacaagcc gataatgtct ttcacctatc aaaaaattac tgtcatctaa cagtgtcact
     7081 agctcgcctt tgactttttc tgctttaggc tcctcaacga tttcttccaa agcagctgtc
     7141 agttcttcgg tgactgttgt ttcctgttca gctgatgatt ttttcttagc aatttctgtt
     7201 ttatgctcaa cgttctgctg ttcattttgt tttgcagaca tgcgttcacc acctatcttt
     7261 tttattatac cttgttttta tttgattggg tagtgagctt tcaagtaatc tcctaccaca
     7321 cttcggataa attcagggaa caaaaattca ttttttccag caatctcaat cgttggaaaa
     7381 aatggaacaa acatcaaatg atctacggta gtaaaagtat aatttttatt cagatcgatt
     7441 ggcttgttct tccaaaagac ttgatggtta atagaatcat atgtgattcc attatagacc
     7501 atctgcccaa aaatcttacc gcgaaatccc attcccttca taggatagtt acgtaaaaag
     7561 ttacgatttt tttctatttc taaaactaag cggatcagat catttccacc caatgtgaca
     7621 tttaacaagt gcatcggatg cggcaacgca gtgtgaagct gatcctgatt gacccgccct
     7681 tttggaattt cagttaaaaa cagaccactg tttaatattg ctgcttccgt ctttcctcgt
     7741 tctttcaccg cctcaaaggc tacttggata aacgaatgct cattgaagat atctagactc
     7801 agagaaaatg gcaaatctgc tacttctttt tcttttaata attcatgccc acgttctaga
     7861 taactattta tttcttgttc atcttctgta aaagctgtca ttgtttcggt agggatggct
     7921 ttggctgaat gcttaaggat ttttttctct tcatcgatgg ttaaatggac ttcaccgaca
     7981 tactgcccgt actttccagc ggctgctatt tggacatcat tgattacgtg accttctctg
     8041 aataaatgat gggtgtgcga tcctaaaata acatcaattg ctggtatttc ttttgcaatt
     8101 tggaaatcat cctgaatccc gagatgactc atcaaaacta aaacatccac ttgtgaacgt
     8161 aaatcttcga ctaattctgg caaaatatca taaggattcc gtatgtccca gccattcgga
     8221 ttataagtaa gcggaaatgg agcagtaaat gcaattagtc cgattttcgt ctggtgtttc
     8281 gttgtaatga ttttatatgg tttcgtccat gccggtggct ggagcgtatt tttatcgaat
     8341 aagttatcca ggatgatatc aaactcagca tgatcgtata aatgattcaa ctcttctttg
     8401 gaatttccta cgccttcatt gtttcctatc gtcgctgcat cataaccaac ttcattcatc
     8461 agcaagatat tttcctgtcc attcgttgct tctgacaatg gatgccaacg atccacaaaa
     8521 tcacctagat ctactgcaat cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt
     8581 tgatccaaaa accttctgat ccttggccaa ttttctaaat gagaatgcag atcattcgta
     8641 tgtaaaatca ctattttttc catgtaccct ctcgcctctc tttatcttta tcataccata
     8701 ggcagctttt tcttccattg aaacaagctc tatttcagag tttttcctgc cttgaagcaa
     8761 attgtataag tacaatattc gataaatcag tctggctaaa aaatcaggaa cattcatttt
     8821 tacagtacta taaaaataaa aaaaacagac ccgaaagtct gttgtcgttt cattataatc
     8881 agacacatgg cggaacctgc ttagtgctgc ttccttccag acctgacacg cttcacaagc
     8941 ccaccattgt cctagccttt cggcaaagaa tagtatacct tatttttcat tcgttgacca
     9001 gtcttttttt aatttctttt ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat
     9061 cccacattcg tcttctaaaa tgccaccttc tacatatgct tggtggttaa accgctcgtc
     9121 ctccaaaaga ttcatcaagg taccggctgt tccgcctttt ggatcatacg cgccgaaata
     9181 aacttctgct acacgagaca aaatcatcgc gccgctgcac ataggacaag gctctaaggt
     9241 aacaaaaagt gcagcatctt ccaagcgcca gcttcccact ttttcacagg cttccttgat
     9301 tgcaagcatt tctgcatgtg tagtagcatc ttgcgtggtt tctctaaggt tatatcctct
     9361 tccaatcact tttccatcca atacaaccac tgctccaatc ggaacttcat gaagcatttc
     9421 tgctttcttt gcttctgcca atgcaaggcg catccatttt tctttttcta ctgcatcaag
     9481 atttgtattt accaattacc cttccctgcc tttgtttttt cttgttttac tttagcatat
     9541 gataaaaagg ctgtaactaa cttttttttc tatggtaaaa taaatacaag aggtgaacaa
     9601 gatgaaaatg gataaattac ttgctcttta cccacaagca cagaaaaaaa actctccagc
     9661 atcagaagcg gatatccttt cttttgcaat tgatgatgct tttatctgga tcaaacaaga
     9721 ttcattatct caacaagaaa ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca
     9781 aaaaaaacac ccatggtatc gctatctttt taaagatgct tcttgtgttc aagaaaaaag
     9841 ctttcgtttg atccagcttc acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca
     9901 agatactatt ttggaattat ttcgctattc tgctgatttc tttttttata cagacacaga
     9961 tgcgctctta gtcgaacaaa taaaccgtga ttatcttgat gctactgaaa tcaacggtat
    10021 ttttctatct ttagatgctg attttgatac gacaacgtct gtattcgttg gttctttcca
    10081 ttcacctggc accgacctcg tccctttgtt tgctgaagaa agaaaaattt ttttaacaga
    10141 gcaaaacaat ctttatactc acagccgtac tttttctatg caagatgtgg ctttacatta
    10201 ctatacaaaa gatactgtaa aagatagtaa gttgatcaaa agttacatag aactgatcaa
    10261 acaaaatgaa atggaagtga tcattcaaga attatggaat gaattaggca atgtcagctc
    10321 tgctgcaaaa acactgtttc ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca
    10381 agaacagtct ggtttcaatc taaaaaaagc caacgattta ctgttttgct atcttttatt
    10441 gcttcatgaa cataattaaa gatagattaa aggggttgtg acaataattt tgtcacaacc
    10501 ccttatttcg aatgaatagt gttcaaaagc tagtttctct tacatcattt tttcaacaaa
    10561 atgcattagc cggtctttta catcttcggt tgaatctttg attgaatcac cataaacaga
    10621 gattttcttt tttcctgatt tgatttttgc taacattccc atcaaagaat ccaagtcact
    10681 atctgaaaga ccatctacga tttctagtaa tttttcattt ccatcaaatt tgtcatagac
    10741 gaattttttt actttcgtcc gagtagtcat atgatggatc ttttccatta cttttcccga
    10801 tacaacgact gctgtcgcta cactggcagc ggctgctacg cttaatccga ttgttacttt
    10861 tgttgatgtt ttcataagta tcagctcctt tttatctatc tccatcatag catagacaaa
    10921 aaaataaacg aactaatcca ctcagtgaaa tctcaatcct ttagggaaaa agttctttac
    10981 tgttcctttt acttgtttcc caaatccgac aggtatctta tctaatacta gcaaaaccca
    11041 tccttgattc ccatcacgtt gaaatgtttc tcctgctgtg taagattgcc attccttttg
    11101 agtgatcggc aagcaaggta tattctctat cttttttgta gcaagcgcta atgcatagct
    11161 aggttcaaat cgattctttt tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa
    11221 accatcgaga gaaggggcta actcaggaac ttcccaaaga tggtcgttga acactaaaag
    11281 tcttccagta gcttcataat gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc
    11341 ttttgtcatt tgcactttac ttttttttcg tgtcttcttt tctttatgca tctgattttg
    11401 tccatgaaaa gtcagctttg caacaaaatg tccttcaccc tgatctttat gaggccatat
    11461 acgtattgtt ttttccaaac cagctacgct tccccattca gaacgtcctg aactaacgga
    11521 ttgagtcaaa ggaatctcct caatcgttac tggataattt tctacaagcc agctaatgat
    11581 ctcttcattt tcttctggtg caaacgtaca agtcgaataa atcagttgtc ctttattttt
    11641 tagcattttt attgctgaag acaaaatctc ttgctgacgt ttttgacagt atagcggaga
    11701 ttcttctgtc cactctttta ttgcatttgg atcttttcgg aacattcctt ctcctgaaca
    11761 aggagcatca accacgatcc gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg
    11821 tgcatgattt gtcactattg cgttcgaaac tccccatcgt tctatatttt ctgataaaat
    11881 cttcgcacgt ttcggaaaga tctcattcgt cactaataac cctttcccct tcatttgagc
    11941 agctaattgg gttgatttac caccgggagc cgcacacaaa tccaaaactt tttcaccggg
    12001 ttttgctgca gcagctgttc caacgatcat tgcacttggt tcttggctat attcataccc
    12061 agcttgatgg aggaaagact ttccattgac agtgccaaga aatccctcat tgctgtaggg
    12121 agcgggttgt aattcttcgc tatggtaagt ttggaccatg tccaatcctg ccggttttag
    12181 tggattccac cgaaatcctt ttttcacgct tccttgttca agcgcagaaa aaaaatcgga
    12241 tgcttcttcg cctaaaagaa gacgatattt tttgataaat tgctgtggta acgttgtcgc
    12301 ttcctccttc attccaatgt ttcgttagac attttacaat cttttttacg aaaaataaag
    12361 gtggctgatc tactttttca tgttgctgcc taaggtgact gttcctttaa gatattttcc
    12421 aagtattcat ccgtcacctc gtttttatcc caaacagcgt tcatcatttc aagaaaatct
    12481 tgagaagaat catctgagcg caaacttccc aatgcattaa gccataccaa ttcaagcgac
    12541 ttttcttctt tgatcttcgt gtgacctttg acatatgaga aaaatggccg cttaaatagc
    12601 gaatccatgt ctaaatcaga ttttgcaatg atttcacgaa tactttcatg gatagaaaaa
    12661 aaatctttca ataagtagac aatgcttaaa acatcatcgg aactgcattc agctaagtag
    12721 ttttcaaaat aaacagtgat ttcttctaat atcttctctt ttaacccgct gattgatttg
    12781 aaatgtgtat aaatcgggta tgtagaacaa ccgcagaact ctgctaactc tcttgcagta
    12841 attgacttca ttccatattc ttccacgtat accatacttt tgaacagcaa atattcttta
    12901 ctataatttg ttactctccc catcattctt acctccgtct cttttcatct acccataaat
    12961 aaataacata aataattcta aaaatattat tgataattat caaaacatat ttagttatat
    13021 ccattaaaaa tcaatttttc cgccttaaaa acaagaatat cttgacaaaa taccctagga
    13081 aataaacaat agaataaaca aggtaacaaa aaaattaaat aacgttatat aaaaaagaag
    13141 gcgacactct gtgagaataa cacagactga aatgactttc cactcacagt ttttgtctaa
    13201 ctatatacaa aaacagaata aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga
    13261 gttacttgtt ctacaaatgg tcattcaaaa aaataattgt tctttacaag aattatccaa
    13321 tgacttacat attccaaaac ggacaatcaa agaactgatc cgaaaaataa atgtatcact
    13381 caagcaattt ttattgattg atgaatttat ttattctaat cataaaggag agatccaaac
    13441 caaagaaacc gatcaaacta aaaagttagc tgttttttct aagttaaaat tatgctacct
    13501 aaaagaatcc aatcgtttta actatttgat gttactaatc aattatccac aaactgctgt
    13561 accaaaaaaa tatttacttg aacaacttta tatatcgccc tcctatctcg aaaaactgac
    13621 aaaacagctc aatcattcct taaaaaaatt ccatttacgg atcctatctg taaaaaattg
    13681 ctgtactctt gaaggaaatg agttatctat ccgtctctat ctttactttt ttctttcaga
    13741 tacatttgga ggtaacgatt ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa
    13801 cagtacagat gagcaaatag aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt
    13861 aactttattc atattacgaa acaaacaaca ggcatatttt ccaaaaactt cttctgaaat
    13921 cactgaattg atggacttat tgcagcatac acatgattat tcagaatact ttacttcttt
    13981 ttcagtgagt cgcccttgtg aagagacaga aacagagaga gcctacttta attatttttt
    14041 ctattgtttt tttccaaatc aaatctcttt tcgacaaaaa caggagattg gttttttatt
    14101 ttccacaagc aaaaacctta tttgtcgaca agtcggtcaa ttgatgactt acttaactca
    14161 acagtttcct ctcaaccatt tagaaaataa aataccaatt tatttttatt ttcttgtgtt
    14221 ggcagttgtt tttttcgaac tgttgggtga atccagcagt agtttttcta cactttgctt
    14281 tccagaagcc acgtccaatc attctagttc ttttatacag gaaaagaaat taagagaaaa
    14341 aatcgataca attttttcaa aaaatgggga aagaaaccaa atcagtcaat accttagtca
    14401 attgatccaa cacttactac aaacagaaac gaaagagcag ttactcatcc atttgcagat
    14461 aaccaaacag tttacaggaa attatcatct tcaaagccat ttgcagcagt tatttcgcaa
    14521 agatgctgtt aaaattaccg catattcaga acatgctgat ttgatcatta cagatcagca
    14581 tgagaaaatt tcagatgaaa cggagacttt ctatctggat acaacgcaca atacagtgtt
    14641 atttggcaag ttaataaaag cggttcatga actgtatatg aaaaaaggtt atgagaacaa
    14701 aacaaagagg atgtgacaaa attcttgtca catcctct
//
LOCUS       DACCCS010000068        14625 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.069, whole genome shotgun sequence.
ACCESSION   DACCCS010000068 DACCCS010000000
VERSION     DACCCS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.069"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            4..816
                     /locus_tag="IVK82_002267"
     CDS             4..816
                     /locus_tag="IVK82_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP6393311.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IVK82_002268"
     CDS             813..1613
                     /locus_tag="IVK82_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP6393312.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IVK82_002269"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IVK82_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP6393313.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IVK82_002270"
     CDS             3110..4819
                     /locus_tag="IVK82_002270"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP6393314.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="IVK82_002271"
     CDS             5031..9389
                     /locus_tag="IVK82_002271"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP6393315.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="IVK82_002272"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="IVK82_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP6393316.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="IVK82_002273"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="IVK82_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP6393317.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="IVK82_002274"
     CDS             11227..11520
                     /locus_tag="IVK82_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP6393318.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="IVK82_002275"
     CDS             11517..11828
                     /locus_tag="IVK82_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP6393319.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="IVK82_002276"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="IVK82_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP6393320.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="IVK82_002277"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="IVK82_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP6393321.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4983 a         2598 c         3255 g         3789 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaatc aggctctttg tcaataagga ctgatgagtt
    14581 gtgcaaaatc aaatctgagt cagaatgaac cacattctgg ctcag
//
LOCUS       DACCCS010000069        14062 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.070, whole genome shotgun sequence.
ACCESSION   DACCCS010000069 DACCCS010000000
VERSION     DACCCS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.070"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IVK82_002278"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IVK82_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP6393322.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IVK82_002279"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IVK82_002279"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP6393323.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IVK82_002280"
     CDS             complement(2169..2906)
                     /locus_tag="IVK82_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP6393324.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IVK82_002281"
     CDS             complement(2997..3524)
                     /locus_tag="IVK82_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP6393325.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IVK82_002282"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IVK82_002282"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP6393326.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IVK82_002283"
     CDS             complement(4401..4886)
                     /locus_tag="IVK82_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP6393327.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IVK82_002284"
     CDS             complement(5039..5236)
                     /locus_tag="IVK82_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393328.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IVK82_002285"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IVK82_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP6393329.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IVK82_002286"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IVK82_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP6393330.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IVK82_002287"
     CDS             9211..9423
                     /locus_tag="IVK82_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP6393331.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IVK82_002288"
     CDS             9425..11104
                     /locus_tag="IVK82_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP6393332.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IVK82_002289"
     CDS             complement(11358..11558)
                     /locus_tag="IVK82_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6393333.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="IVK82_002290"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="IVK82_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12249..12322)
                     /locus_tag="IVK82_002291"
     tRNA            complement(12249..12322)
                     /locus_tag="IVK82_002291"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12286..12288),aa:Ile,seq:cat)
     gene            complement(12634..13032)
                     /locus_tag="IVK82_002292"
     CDS             complement(12634..13032)
                     /locus_tag="IVK82_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393334.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            complement(13025..13477)
                     /locus_tag="IVK82_002293"
     CDS             complement(13025..13477)
                     /locus_tag="IVK82_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393335.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            complement(13464..13871)
                     /locus_tag="IVK82_002294"
     CDS             complement(13464..13871)
                     /locus_tag="IVK82_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393336.1"
                     /translation="MSFLLLKYLEIYSVKKNKLIGENDSPIKTPIELSLRNRMGILQR
                     SEIVKELFYHKFSYEIRLDDTYEHYRIVFFVYNSIEDATATTALTFGFTKNGTINSDK
                     TRQAATESDDICKKVCNGEENYWIGEEKLNEIY"
BASE COUNT         4150 a         2947 c         2368 g         4597 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 tgccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgacatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaactg agctaagagt acaggttgtt gccacataaa gccataaaca atcaaccagt
    12361 agaaggtgtg gcaacaatcc tgttatcgca tagcttggag tgtgactatt atgggtgata
    12421 gtgaagatat gcgataacat cactatttta tcgaatgatt tttatagttg tcaatacagt
    12481 catctaaata cttcatatca atcatttatt cagaaacaaa ctatcttgca ggatctatac
    12541 gagttaatta cagagcaaaa aagcctcaac caaggatgac tggttgaggt ctttgtcttg
    12601 caaatttaaa tagataactc tattacttgt attttatttt tgttgactat atagctgata
    12661 taaatcaata actgtaggca taactcctac ttcgctatat aaattagaaa tcaaattaga
    12721 agctttattc aggataggtt tagctagctc tcccatagat tcttctacta attttttata
    12781 tggctgttca tttgtagaaa cacgaataac tgaaaagagt aaaatatcta ccggtgctcc
    12841 ttccatcccc cctacaatgt gagctcgtaa agaaacaagt tttttttcta gatactctgc
    12901 ttcgtcttct ttatatccaa gttcaatatt aaactctaaa tctccataac taaaattatc
    12961 agttaattca aaatgaattt tattcaaact aatattttca agtgcaaaag taacactatt
    13021 tcctttatcc atgtacatat acctcttttt ctacaatata gcggctcgta tcttggtaat
    13081 taacactagc atagaccata tttgacgata cttcttttct ctttctactt ttaaatactg
    13141 agtaactttt attccgaata taaggtttat tttcaatacg gtcatattta atagggccgc
    13201 cagcaacctt aatagtgaaa ttcattgaat ctttatagga caaattttca acttgtttca
    13261 aaagtttatt gtactctgca acaggaatat gcatatctcc gaactcaaat tcaagatatt
    13321 ttttttcact tattcctaac atttttgaga attctgtcac gtttttttgt ttttttaatc
    13381 ttattttaag taattgtttg gacaggtttc tttgttcaga atttaatttt ttatataaat
    13441 caggatgttt ttttaataaa tcattaatag atttcattca acttttcctc ccctatccag
    13501 taattctctt ccccattgca tactttctta catatatcat cactttctgt agcagcttga
    13561 cgggttttat ccgaattgat tgttccattt ttcgtaaaac cgaatgttaa tgcagtagtc
    13621 gcagtagcat cttctataga attgtatacg aaaaaaacaa ttctataatg ctcataagta
    13681 tcatctagtc ttatttcata actaaattta tgataaaaaa gttcttttac tatttccgat
    13741 ctttggagta ttcccatccg atttcttaag cttagctcaa taggtgtttt tattggagaa
    13801 tcattttcac caattaattt atttttttta acagagtaaa tttcaagata ttttaataga
    13861 aggaaagaca caattatggt ttaaatttga tccttcgaat agatttatca aagattttta
    13921 taaggtgtgg gattcagaag ttttctttct agcaatcgaa gatagcttat taatcaatct
    13981 ctactattct aataagaact actttaaaat ccctgctgca aaaactagaa tgaagaaaga
    14041 tgtatacttt ttgtttgata tc
//
LOCUS       DACCCS010000070        13853 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.071, whole genome shotgun sequence.
ACCESSION   DACCCS010000070 DACCCS010000000
VERSION     DACCCS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.071"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            5..2377
                     /locus_tag="IVK82_002295"
     CDS             5..2377
                     /locus_tag="IVK82_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393337.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="IVK82_002296"
     CDS             2438..3898
                     /locus_tag="IVK82_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP6393338.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="IVK82_002297"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="IVK82_002297"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6393339.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            6354..6947
                     /locus_tag="IVK82_002298"
     CDS             6354..6947
                     /locus_tag="IVK82_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP6393340.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6960..7859
                     /locus_tag="IVK82_002299"
     CDS             6960..7859
                     /locus_tag="IVK82_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP6393341.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7862..8335
                     /gene="ssb"
                     /locus_tag="IVK82_002300"
     CDS             7862..8335
                     /gene="ssb"
                     /locus_tag="IVK82_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6393342.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            8701..8961
                     /locus_tag="IVK82_002301"
     CDS             8701..8961
                     /locus_tag="IVK82_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393343.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
     gene            9406..9612
                     /locus_tag="IVK82_002302"
     CDS             9406..9612
                     /locus_tag="IVK82_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP6393344.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9612..9863
                     /locus_tag="IVK82_002303"
     CDS             9612..9863
                     /locus_tag="IVK82_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393345.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTTETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTELEN"
     gene            9879..10295
                     /locus_tag="IVK82_002304"
     CDS             9879..10295
                     /locus_tag="IVK82_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393346.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            10277..10549
                     /locus_tag="IVK82_002305"
     CDS             10277..10549
                     /locus_tag="IVK82_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393347.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            10716..11117
                     /gene="tnpA"
                     /locus_tag="IVK82_002306"
     CDS             10716..11117
                     /gene="tnpA"
                     /locus_tag="IVK82_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP6393348.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            11129..12274
                     /gene="tnpB"
                     /locus_tag="IVK82_002307"
     CDS             11129..12274
                     /gene="tnpB"
                     /locus_tag="IVK82_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP6393349.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            12512..12775
                     /locus_tag="IVK82_002308"
     CDS             12512..12775
                     /locus_tag="IVK82_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP6393350.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12769..13119
                     /locus_tag="IVK82_002309"
     CDS             12769..13119
                     /locus_tag="IVK82_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP6393351.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(13143..13757)
                     /locus_tag="IVK82_002310"
     CDS             complement(13143..13757)
                     /locus_tag="IVK82_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6393352.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         5169 a         2277 c         2799 g         3608 t
ORIGIN      
        1 ggacatggca gaagaaaaag aaccactaga caatagtcgc ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaacga acaaattgag aagatgtttg aacaccagag
      121 aaagaccaac ggtcagccaa tgaacgataa gagaaatggg cattcttggt ttaggcaaca
      181 agaacgttta gaaaacaaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      241 ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 cctggatatg acaatcgaaa atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat tggaaacatt
      421 agaacagctg aaagaaagat cagaaaaagg aaaagaaaat cttcttcctg aagtacaagc
      481 aattattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 agactatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctgc
      661 gccaactgat tctttgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tagaattgat
      781 cgatcaactc aaaacgttgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccca tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 aaaggaacaa aaacaaacgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaagcaaga
     1081 agtcaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtgaaa gaacttttga attttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ctgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actacacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgggg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cttcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 ggcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctgtagaaac ggcagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtga ttttaaaagg
     2221 aaaaagttat ggaaaagaga ttcaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg aaacagtgtc agaagtggcc aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg cgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg tttgaaaatg accgaaaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagact atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ctctaagcaa caatttaaag cagccacaga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtagaa aatcttcatt ttgagacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaac atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaat gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagttaaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctaaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtttcga tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagttga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaagtcattt atgaagaagt cgcaaaagaa gaaaagatca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaat tggtttccgc
     3541 tcacaaagta aatgcgtctt ttggtcagca gctttctgtt aacgattcaa tagaagctat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagcttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg ctcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggatt ggaatgatca attgaagcat gaaatctacc aacaagagat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggtaga cagcaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaaaccgtc acaagcgaag gcttatgccg
     3961 atagcttttc taaagctacc cgaaaagatg ggtattttga gatccaagat cgattgtttt
     4021 caggagaaac tgtgatcacg tatggttttg gtcatttagt cgagttagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagagcattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatagctt
     4261 ggacgatcat tcaacaagca ggcgccgatc atggaaagat atttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagat gcagaagaga
     4381 cctacccaaa atttgaagaa gcacaagcac gccagatcgc cgattggttg atcggcatga
     4441 acggtagccc gctgtatagt ttactcttgc agcaaaaagg cataccagga agtttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attacaggaa aagattagaa
     4561 acttccaaaa agaaccatat tttgaaggaa aagcccaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggtac gatccaccaa gttgaaaccg
     4741 agaaaaaaag tgcggcaagt ccacgtcttt tttctctatc cagccttcaa tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagata ctttggaagc catgcaaggg ttgtatgaag
     4861 ggaaatatct aagctaccca cgaacagata ccccttatat tacagaaggg gagtatgcgt
     4921 atctgttgga tcacttagac gaatacaaac actttttaaa ggccgaagcc attcctactc
     4981 ctattcatac accgaatagt cgatatgtga ataataagaa agtccaggaa cattacgcga
     5041 tcattcctac aaagacggtc atgacggcag cggcttttga acaattaagt ccactgcagc
     5101 aggctattta tgaacaagtg ttaaaaacaa cggtggccat gtttgcggaa aagtatacct
     5161 atgaagaaac aacgatcttg acgcaagttc agcagcttca gctaaagaca gtcggaaaag
     5221 taccagtaga tctaggttgg aaaaagctgt ttggcaaaga gagcgacaga aaagaaaagg
     5281 aagaagaacc gctacttcct aaagtgacaa aaggagaagc ggtgacggta gatctacaag
     5341 tccttgaaaa agaaacgaag ccaccacaac cttataccga aggtacgttg atcacagcga
     5401 tgaaaacagc tggaaaaacc gtggatagcg aagaagcaca atccatattg aaagaagtag
     5461 aaggaatcgg tacagaagca acacgagcaa atattattga aactttgaaa caaaaggaat
     5521 atatcaaggt agaaaaaaat aagcttgtgg tgacaaataa agggatttta ctttgccaag
     5581 ccgtggaaaa agagccgctg ctaacaagtg ccgaaatgac cgccaaatgg gaaagttact
     5641 tattaaagat tggtgagcag aaaggaacac aagctacttt cttagctaat atccaaaaat
     5701 ttgtctctca cttgttggaa gtcgtaccgg gacaaatcca gtcaacagat tttggatcaa
     5761 cgttacaaga ggtgaaagca gccagtgaaa aacaggaagc aacgcgtcat ttaggtgttt
     5821 gtccaaaatg tcgggaacaa gaagtcttgc tttatcaaaa tgtggcggca tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gccacacaac
     5941 tgaaagagat cattcaaaac ggccgaacaa gccaatcagt cagaggacta aaaggtcaga
     6001 aaggttcgtt tgaagcaacg atcgttttaa aagaagactt tacaacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac tataagaaaa gaacacgccg cactacaaaa taatgaaggg
     6121 gttatcctat gcgaaataca acacacaaac aacaagaaat cgaaaatcaa aaagcaaaaa
     6181 ttcgtttaga acaagagaaa ttacgtgcaa ataaaagaag actgcaacaa ttaaagaacc
     6241 agcagcgcat tcaaaaagaa atcgaattag atcgatagac gttaatcttg ttacaaataa
     6301 actactatga tatatttgat gtagtgtatt tgtaacgaag ggaggggaag taaatgcaag
     6361 ataaactgaa agaattgttg gctattcgcg atggaaattt atctatgaag gaagccagga
     6421 aatttggaat cgcagcaacc actgtacaaa gattggtgaa tcaaggaaaa ttgatcaaag
     6481 ttgatcgtgg ttattatgta gaggatggtc atggaataga tgatttattt tggcttcagc
     6541 aacgattttc caggggaatc ttctctcatg aaacgacttt agatatttac cagctttcta
     6601 cgaatatgcc gaaattcatt cacttaactt ttccccatgg ctataacgtt gatcgttcaa
     6661 ttacaaaaga gcagcagtta caatttcatt tcgtgaagaa agaagtttat gaactaggaa
     6721 aagtagaggg aacttccttt caaggaaatc ctattactat gtatgataaa gagcgtacac
     6781 tttgtgatat ttggaatccg agatatgaat ttgaatatga aatgaaatta gaagcattaa
     6841 aagagtacat ggaagaccca ttgagagatc caagtaaatt aagagattac atgacaaaac
     6901 tacatgtaga aaaagaaatg aaataccaca tgcaaagctt atattgatgg aggaaaaaga
     6961 tgacaccgac acaatttgca gcaaaaacaa gaaatttagc aaagagtaca ggattaaatg
     7021 cacagttagt gcaacggcat tttatgatgg atcgtctaat tgaacaaatt tctgaatcaa
     7081 agtacaaaaa tgacttcatt ttgaaaggcg gctttttatt aggatcaaaa tatggaatcg
     7141 atagaaggac aacaattgat atcgatacga catttagaaa tagcaaatta actgaagaaa
     7201 aattgaagag catttttaat gatttaacta gaaaacccac aaaagaaggc attgttttct
     7261 ctattcaagg aatgaaggaa acaagagaag ccgattttta tcctggattt caaattaaat
     7321 tggtggctaa tttagagaaa gtacgtgtac cgtttaaaat ggacgtgaca acaggagatt
     7381 ctatttatcc tgaagtagat acacactatc accaattaat gtttgaagat aaaaaagtgg
     7441 agattccagc ttatccaaca gaacaaatta ttgcagaaaa gctaagtgct acgttcagct
     7501 ttggcacaga taattcgaga gcaaaagatt actacgactt atttaccatt ccgaaactag
     7561 aaaatatcga tacgaaagag ttatacaaaa gtgttcgaaa tacatttaca aaacgtgggg
     7621 atacaaaacc gttgtccttt tattatgaaa gagaaatgga taagatcaga ggaaatgact
     7681 atttggaaaa gcagtgggaa aaatatcgct cagttaacac gtttgcgaat tccatttcat
     7741 ttacagatac agtcaacgaa attgatcacc taatgaaaag tgtgatcaaa attgaaaatc
     7801 aagaaagaaa acggcagttt aaaagatcac aacaaatgaa tcaagaaatg gagagataaa
     7861 gatgattaat aatgtgacat tagtaggacg cttaaccaaa gatcccgatt tacgttatac
     7921 tgcaagtggc acagcagtag cgacttttac cctagccgtc aaccgaaatt tcgcgaatca
     7981 aaacggcaat cgagaagccg attttattaa ctgcgtgatt tggcgcaaac cagcagaaac
     8041 aatggcgaca ttagctaaaa aaggcggctt gatcggtgtt gtcggacgga ttcaaacacg
     8101 aacttatgac aaccagcaag gacaacgtgt gtatgtgact gaagttgtag cagataactt
     8161 tcaactatta gaaagtaaag cagcgacaga gagccgtgcg cacgctgatc aatcaaccac
     8221 cagtccaagc cccactacct ttgaacaacg ggatacaaac aatggattgg acgcttcaca
     8281 aaatccattt ggtggacaat caatcgatat tagcgatgat gatctaccat tctaagtagc
     8341 attttctatt ggttgcattt gctacggttt atgttataat ttagtcaaca aaaagaattt
     8401 tcaatctata accaaaaacc actagctggc gggctagtgg ttttctttta cgtttctatt
     8461 ataagaatta acgggtaggc ttactgtctt tcttggcaaa tttacaatca agccatttgc
     8521 agatatagtg cgcggctact tctgccatga ttgcaaaaac aaaatcaaaa agaattttca
     8581 aacctataac ctcctcccct tgccagatta ggccggggta gcagacagca agactattat
     8641 agcataattg aaatattcaa atgattggat ttttgctata attttagcga ggtgattgtg
     8701 atgaacaaat atgttggttt gcttgataaa atccgagtaa taaaaacgca accgttattg
     8761 gttcgcttta ctttacaaac gattcatgaa tcgattaatt gtgtagttgc agatattgaa
     8821 attatcgata aattgctaat tatggatgat ggaaaataca atattgccgt cacaggtcac
     8881 ttcaataaac gaaaccagct agttattgct agcatgtatg ttagaaatcc cgatcagttt
     8941 acgcgattga tgggcatata aaaaaagaag agttgcaatc ctaatgtagg actgcagctc
     9001 tttttgtttt acatagaaac aagaaaaatg aaaaggcctt tttaggaagg cgcgagcgcc
     9061 ttctcaattg aaaaacctct ctcactccaa ccgccaccgc cgttcgagaa gaaaggaagt
     9121 gaacgttgca cgttcactaa gataaagggt cttgcaaagc aagacaaaat agaaaaaact
     9181 tcctctcacc aaaaggttcg ttcctgttcg ttatctcttt attgatttgc cagaccggca
     9241 acaagttgcc ttttatcacc aaaaagcgac aaggacaagg gtaaatggca cgctgcgcgc
     9301 ccttgtcctt gtcgcttttc gttgattcat tctggccaac aaatcaataa agaaagaact
     9361 aacgaaagga agtaaaacct ttcctaagag gaggcagaat caatcatgcg aaagaaattt
     9421 gattattggg gtgttccctt cagcgaatta ttcccaaact atcatgcacc acatactgtc
     9481 gaatgcgatt gtggagaaag agcgaaatgt atcaaaagct atcgtttgta tcaatgtcca
     9541 acatgcggga aaaaatatac cttgtcttat ggcgattata ttttgattga tgagaaagga
     9601 aagaaacgtt gatgaaaaca caacacaaat tattgactgt aaaagaagtc gttcaaattt
     9661 tggtggacaa agaagtcatt tctgaaaaag tagcagaagc tttacttatt ccgcggtact
     9721 ggacgacaga aaccccttta gtagaggatg caccgactat gctatttgaa gacctagtga
     9781 agattgcagg atatgaactg cattatcaag aaaacttgga aacttatgaa aacgaatatt
     9841 ggttaaccga attagagaac taaaaaggag aaaaaaatat ggctcaaaat tactatgacg
     9901 aatttgtcaa attacctttg gataaaatgg cacaaaaaat ggaggatatg acattcctat
     9961 acaatgagac acgtgttcca aaaaaacatt ataaggaaag attgtcggta gctgtggaag
    10021 aaatgattga atcaggtgtc gaaatgaatc tcattgcgac gtattatcga acactggaag
    10081 aactgaaaaa gcaaaatgca aaatggtttt tccaagcctt actatgcttg gaagtaggcg
    10141 tcaaaccaag cacaatcaaa ccttctgaat accaagcttt agaactaacg tatgcgaaat
    10201 ttatcgaaac gaaaaaagct aagactgttt caagtgaatg gttagattat tttgaaaata
    10261 tcaataagta tggcgcatgt tacatcatga agtaatcaga cggattcaaa aagactttag
    10321 ttgtatggaa gaaatgaagt actttgttca aaatgttgaa gaacagttat atttggaaag
    10381 cgaaacgagt caaacaaagg tcacagtcag ccataatcgt ttagatgtgt ataaacgtcg
    10441 tcctgaatca ttaaaaaaag gagcggagct tctacgacta gaaacagaaa aatacgtcat
    10501 gaatgtttac ctagaaaaaa gtgagttggt cgaaaatttt atggactagc cgagaacacc
    10561 cgtgatttta atcatgggat gaatcggatt cagttgtgac ctagctttag ctaggttaaa
    10621 caactgacaa atataaacta aataatagtg aaatgtcttg caataattga ttttgtagaa
    10681 atacatatat taaaataatt gcgaggtgtt tttgtatgtc agaaattcat tatggacggg
    10741 gttatgtcta ttctattcaa tatcatcttg tttggtgtgt aaaatatcga catgatgttt
    10801 tgtatggtca ggtagatatt gatgtcaaac agtttttgaa acaaattgct gatgataata
    10861 atataaaaat tatagaaata gaatcagata aagaccatat tcatttactc attgaatgca
    10921 ccccacaaca ctatattcct agtattgtta aagtatttaa aggcgcatcc gctagactgc
    10981 tgtttaaaaa acatcctgaa cttaaacaac tcctttgggg tggacatctt tggaatccaa
    11041 gctactttgt agctactgtt agcgaaaata cagaagaaca aattagaaaa tatatacaaa
    11101 atcagaaaaa gaaatgaggt gaatccgtat gctacaacat agggcttata aatttagaat
    11161 ctatcctaat caagaacaag aaatattaat gactaaaact attggctgtt caagatttgt
    11221 ctataattat tttttgaatt tgtggaatca tgaatatacg actactggaa aaggattatc
    11281 ttataattct tgttctgcca tgcttccaca aatgaaaaga aatgcagaga caatttggtt
    11341 aaaagaagtt gacagtattg cgattcaatc ctcattgaaa aatctttctg atgctttttc
    11401 tcgcttcttt aaaaagcaaa ataaacgacc acaatttaaa agtaaaaaac atcctataca
    11461 aagttataca actaaaaatg tgaataatag tatcgaaatt agaaacaatt tcgtaaaact
    11521 acctaagtta ggatttgtta aatttgcgaa aagtcaagag cccaaagggc gaatcttaaa
    11581 tgctacgatt cgaaaaaatt cgagtggtaa attttttgtt tccatcctgt gtgaagaaga
    11641 aatagttgag tttcctaaat ctaattccgc tattggaatc gaccttggta taactgattt
    11701 tgcaatcctt tctgatggtc aaaagattga taacaataaa ttcacgtcaa aaatggcaaa
    11761 aaaactcaaa cgtgaacaac gtaagttgtc aaaacgtgca ttactagcta aaaagaaagg
    11821 cgtcaatctc tttgaagcta aaaattatca aaaacaaaaa cgaaaagtag cacgactaca
    11881 tgaaaaagta atgaatcaac gcaatgactt tttaaacaag ttgagcacag agattatcaa
    11941 aaaccacgat attatctgta tagaagactt aaatacaaaa ggtatgttgc gtaatcataa
    12001 actagcaaaa agcatttctg atgtgtcttg gtctagtttt gtgactaagt tacaatacaa
    12061 agctaactgg tacggtcgga aaatcatcaa agtagataaa tggtttccat ctagtcaaat
    12121 ctgttcaaat tgtggacaca aagacggcaa gaaatctcta gaaattcgag aatggacttg
    12181 tcctgtttgt catgctcatc atgaccgaga tatcaatgct agtatcaata ttttgaccga
    12241 aggtctaaga ttacatgtca tgggattagc ttagatccaa tcatcaaaaa ccgtaggaac
    12301 tacggggata gcttggtaaa taagagaaac cgctgtgagt aaagaaatac gcttgcaagt
    12361 atgctctatt cccaagaatc tcgtgacttt agtcatgaga ggttcaagga ttcatggaat
    12421 gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt atgtatgtta
    12481 tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa aaaaatgggg
    12541 aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa ttcatacaga
    12601 agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa ttatcgaagc
    12661 ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca ctgatgaaag
    12721 aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat ggtgagaaaa
    12781 cctagacaag gcgatattct attattgaat acagcacctc gatctggaca cgaacagaca
    12841 ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag taacgttgtt
    12901 acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa tataaactca
    12961 gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac aattgattat
    13021 gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga ggagttattg
    13081 atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag cagcatgatc
    13141 aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg tctcgtaaca
    13201 ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc aacgatgttt
    13261 ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt aggacgtcct
    13321 ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg tttagattcg
    13381 gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt aatgaattta
    13441 tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat gatcagctgc
    13501 acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt tcttccaagt
    13561 cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag tgcttcttga
    13621 aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat tctctctgca
    13681 ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga tgagacacgt
    13741 atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa acaccctaat
    13801 catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg gta
//
LOCUS       DACCCS010000071        13817 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.072, whole genome shotgun sequence.
ACCESSION   DACCCS010000071 DACCCS010000000
VERSION     DACCCS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.072"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            95..1045
                     /locus_tag="IVK82_002311"
     CDS             95..1045
                     /locus_tag="IVK82_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6393353.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            1563..2018
                     /locus_tag="IVK82_002312"
     CDS             1563..2018
                     /locus_tag="IVK82_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6393354.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2329..2685
                     /locus_tag="IVK82_002313"
                     /pseudo
     CDS             2329..2685
                     /locus_tag="IVK82_002313"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2698..3108
                     /locus_tag="IVK82_002314"
                     /pseudo
     CDS             2698..3108
                     /locus_tag="IVK82_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            3223..3387
                     /locus_tag="IVK82_002315"
     CDS             3223..3387
                     /locus_tag="IVK82_002315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393355.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            complement(3538..4517)
                     /locus_tag="IVK82_002316"
                     /pseudo
     CDS             complement(3538..4517)
                     /locus_tag="IVK82_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(4522..5289)
                     /locus_tag="IVK82_002317"
     CDS             complement(4522..5289)
                     /locus_tag="IVK82_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6393356.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            5779..6204
                     /gene="lacA"
                     /locus_tag="IVK82_002318"
     CDS             5779..6204
                     /gene="lacA"
                     /locus_tag="IVK82_002318"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP6393357.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6221..6736
                     /gene="lacB"
                     /locus_tag="IVK82_002319"
     CDS             6221..6736
                     /gene="lacB"
                     /locus_tag="IVK82_002319"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP6393358.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            6747..7679
                     /gene="lacC"
                     /locus_tag="IVK82_002320"
     CDS             6747..7679
                     /gene="lacC"
                     /locus_tag="IVK82_002320"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP6393359.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            7682..8662
                     /gene="lacD"
                     /locus_tag="IVK82_002321"
     CDS             7682..8662
                     /gene="lacD"
                     /locus_tag="IVK82_002321"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP6393360.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            8690..9007
                     /locus_tag="IVK82_002322"
     CDS             8690..9007
                     /locus_tag="IVK82_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6393361.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9007..10713
                     /locus_tag="IVK82_002323"
     CDS             9007..10713
                     /locus_tag="IVK82_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6393362.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            10855..12261
                     /gene="lacG"
                     /locus_tag="IVK82_002324"
     CDS             10855..12261
                     /gene="lacG"
                     /locus_tag="IVK82_002324"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP6393363.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12284..12556
                     /locus_tag="IVK82_002325"
     CDS             12284..12556
                     /locus_tag="IVK82_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAP6393364.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            12577..13476
                     /locus_tag="IVK82_002326"
     CDS             12577..13476
                     /locus_tag="IVK82_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP6393365.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4481 a         2316 c         2679 g         4341 t
ORIGIN      
        1 acataagaca aaatgttttt aaaactgata acgtatttat tccacaacaa ttacgcattt
       61 atttctgaga aaaatataaa gcaggaggat tacaatgctg aaaacttatt ccttaacaaa
      121 agatacatta ttagtttcgt cagaatcaaa tatcaaaaat tcttctgttg tttatgttta
      181 tggtgacgaa cgagaatata tcgaatcttc tgaacataaa tatgacttta aaataggcaa
      241 tatactaacg tttgatgatc gagttgcata tgacacgatg atagataaga acgatgaaaa
      301 aagtttgctc gtaagttttt tgtttcccga ggtacatgaa ggtgggcatc tagataccct
      361 gacgacatct gttgttttct tggtatttaa aaataagttg ataattttta caaaacaaaa
      421 attaaaattc attcccgaac tcttatccaa tatggtttta cgtgatgtca atcatttcgt
      481 ggaggaagta cttttaaaga tcatgcaaaa agatttctat atgatgctta acgaccttcg
      541 tggagtcaag gaaaaaattg atgatttaga ctcagaaata tcaacgtccg gttcactcaa
      601 accaacgttt ggtgatttat tgactcttca aaaatacatg atagctttgt caacaactta
      661 taaagctaac cataaagcgc ttgatttcat taaaaaaaat ttcacaacta ttgacgatat
      721 tgattttagt actaaaaaaa taattgatga tttatacgat acaagtgata caatggatag
      781 aatcatttta agttacagcc aatatttgga caaattggaa aatatgataa acaatatgat
      841 ttcgtatcag ctaaacatga ttatgaaaac cctaaccgag atatcaattg ttttgactat
      901 tccagcaatt atttttggct tttggggaat caatgtccat gtcccgtttg aaaagtcttc
      961 ttatggtgtt ttagccgtat tgattatctc tgctatacta agtctaattt gctggctttt
     1021 gatgcgacgc aaaacatatt tgtaaagtat ttgtgatacc ctaatatgat ttaaagcaga
     1081 atttctttgt tgacttttta aaaggctgat tcctttcaat aatttaatag cttcagcggc
     1141 tgctcagaag tcaataaatt ggactatttt tattcagtta cttaggcaac atttgagata
     1201 cgattgcgat tataaaaatt gatataccaa ccaatggctg cttggaagac tgacatatta
     1261 gcctcgatat catcgaataa ctaaatacag aaaaatacaa taaatattat caaactaggg
     1321 actatactag aaaacatcag tacattcctt aactcagggt ctataattag aaaatcgccc
     1381 ctttgaaaac tgattaaaac agcccccatt atctagtgac tagataatgg gggctgtttt
     1441 tttgttaagt gatataataa accataaaag aagtttagaa atacggtcct ggtcatcaat
     1501 aaataaacaa tttaattaat tatttattta attatttaat ttcagaaagg tatacatgaa
     1561 tcatggtgca acaaattgtc ctacccatca aagactccag cgtcttaaag atggtacaag
     1621 atacacttct cgatagtttc cgtgctggtc gccgaaatta caccgttttt caagttggta
     1681 aggccacgct actacgtgtg agtgatgtca tgacattaaa aaaatcagat gtctataatc
     1741 cagacggctc tgtcaaaaat gcaaccttta ttcatgataa aaagaccggt aaagcaaaca
     1801 cgttatattt aaagcctgtt cagcaagact tgttgcaata tcatgattgg ctggcccaag
     1861 aaaatatcaa ttctgagtgg ttatttccct cgacagccca ttatgatctt catatcactg
     1921 agaagcagtt tcataaggtg atggcgcatg ttggtgatct cttaggaatc aacttggaag
     1981 acaaagagaa aaaattgcga gtcagaaaac cgttttgaga tatgaacagc atttgccttc
     2041 acgccttagc gattttaaat aatagccttg aaagtgatta tggaaaagcc ctctacgctc
     2101 aatgcaaaat tgacgtagaa actatttttg atcgattgaa aggtgtattt ggtatgtgaa
     2161 gaacccatgt cagaggcaag aacgttgcac ctactgacat tggaatgatg cttatgagca
     2221 tgaatctaac caaattatcc cttgaggcta gaagaaaagc ggaagctttt caacacaaag
     2281 cagccaaaaa taaaaaacgc gacgaagcaa tcacatttat gattattcat cgcgtttttt
     2341 atatttagag ctagttattt gctagaccca ctatttacag atgaaacgtt tatcaccttt
     2401 ggttttgggc atttagtgga attagcagaa ccaggtcatt atgacgaaaa gtggcaaaat
     2461 tggaaacttg aatctttgcc gatttttcct gatcgatacg attttgaagt tgcaaaagat
     2521 aagggaaagc agtttaaaat tgttgcagaa cttctcaaaa aggcaaatac aattattgtt
     2581 gcaacagata gcgacagaga aggtgaaaat atcgcctggt cgattatcca taatgcaaat
     2641 gccttttcaa aagataaaac atttaaaaga ctatggatca atagcttaga aaaagatgtg
     2701 atccgaagcg gttttcaaaa tttgcaacca ggaatgaatt actatccctt ttatcaagaa
     2761 gcgcaaacac gccaaattgc cgattggttg atcggcatga acgcaagccc tttgtatacg
     2821 ttaaatttac aacagaaggg cgtacaaggt acattttcac taggacgtgt tcaaacgccc
     2881 accttatacc ttatttttca gcgccaggaa gccatagaga attttaaaaa agaacctttt
     2941 ttcgaggtgg aagctaagta aaacaagggt catttaaggg cgttctaagc cccacacagc
     3001 gttttaaaac ccaagaggag ctttttagct tttgtttctt ctaaacaagc taaaataggc
     3061 aatcaagagg ggataattgc tgatgttcaa accaaagaga agaaaacgaa tagtccgagt
     3121 tcgttttctt taagtagttt gcaatcaaaa gtcaatcagc tttataaagc gacagcgagc
     3181 caaactttaa aagctatgca aggactgtat gaagcaaaat tattgagtta tccaagaaca
     3241 gatacaccat ttattacaga gaacgaattt gcttatttaa aagcgaattt tggcaaatat
     3301 agcggttttt taggacttga tcttgaaatg ttgttatcat tgataatgat acgttcaaac
     3361 aacagcaccc aaattttgac gaattagtga aactctatga aaaagacgta gtaaaacatg
     3421 caacatccta ttctaatcag ctggtaaagc ttaattaggt tatataagct tgaaaataaa
     3481 gggtttgtcc aatatggaca agcctttttc ttatgttttg ccacagattt gccacactta
     3541 ttgtgtaaga gtatcaagga taggtataat ttgatacttt gtttttttag tgatgtgcaa
     3601 ataaatttct gttacacgac tacctctaga atgccctaca cggtcttgaa ttgcttctag
     3661 gggtattcct tactctgcta agaaactaat atgcgtatga cggaatatat gggtcgtaat
     3721 atgtttatta aaactaagtt tattatcaat atcaattcct gctcgttttg gattaccttt
     3781 tcgatttaat tttccatttt caggtgagtt gaggaaattg ttaaggacac tataatgaag
     3841 aggtgttcca ttatagtcgg gtttatgacg acttttggaa gtaaaaatcc atccattatc
     3901 tttataatca ggattgaatc tcatatgttg gtgatttaac tcaatttcct ctttaagaat
     3961 ttctatagcc cttttagata attggacatt tcggtaactt tgtaagtttt ttacattacc
     4021 ttcatagcta ttttctgcag ctcctccgtg agtggactga ctaatgatga tattttgctt
     4081 ttcaaaatat tgataggcac caatattttg gagtttcgac tctgtaagtc aacagggcca
     4141 ttagttagaa tacctattgg gatgcggttt ttatgtagag ccagcagtag ttgatgtatc
     4201 tcaggaatca tcgtaatctg atcgagttca tattcatagt cagcttggaa gtctagagct
     4261 tctgtcctag aaatactatc gatgccaaat tcttccatag tcttctgaca gcgaaaaatt
     4321 ctaagctgct caatactgat gagttttttc gtgtatttag gaaaagcaac atcagaccaa
     4381 tatctgaaac gtttgtaaat gttatctatt tgttggatat caatcgttgg aaaacatttc
     4441 tcaacgctac gtttaaaggg gagttgatgg tcataaagag tgtcatcaag gtcaaaaaca
     4501 atatttttat aagtcataat tctagtccgc ttttaaaata gtagtgtact ggctaaattc
     4561 ctctaagtgt tgagggctaa tctggttatc tgtaatgatg gtatcgagtt gatctagatt
     4621 atagaagtta aagaaatcgt atcgatcgaa tttagtactg tctactaata agaatttttc
     4681 tacagcattg tttagggcaa gttgttgaat cacaccttcc ttgtcactat atgtagcaat
     4741 agaattatgg gtaacagcat ttgcactaac aaaagctttg gcaaatctaa ttgcttttaa
     4801 atttgtcgaa gccattgaac ctacaaaagc tccagttatt tctctatatt caccgccaag
     4861 aagcaataaa tcaatggttt cgctatcatt tagaatcaag aacacaggga gactatttgt
     4921 aatgacacga attttataac ctttacgacc tttcaattct aatgccagtt gttcaagtgt
     4981 agttccaggt ccaataaaaa tagtatcgcc atctttgatt aaagagcagg cttttttagc
     5041 aatgtcaatt ttttcttttg tatttagact tttcttctcg atatgtgtct tttcaagtgg
     5101 ctttttagag gaaagcaatt gtgcaccacc atgagtacgt gttaaaagtc catcagcttc
     5161 tagagcatca aggtctctac gggctgtcat atcggaaata tctagttcat ctattatttc
     5221 ttttatggtt atggtcccat caatctttaa catatctaaa attttttcta atcgtcgttt
     5281 tttgttcata tgaagaccct tctttcataa agtaattttt ttccaaagat aattctcttt
     5341 taattgtatc ataaaagaca atattttcaa ggtaaaacaa acaatttcaa acaaaaacaa
     5401 atgttagatg atgaaataag aacagaggat tgacgtatat tagcttaggt cagattttgt
     5461 ataagacgaa aataaagtag gacctcttaa tcagtaagtt atagaaagta aaagactttt
     5521 gtaatacttg aatagatatt tcacgtccat tttgtgatgg attaaatgaa caaaaatgaa
     5581 caataattta acggtgttat ctatttttta aaaaaacaaa taaaaaaaac aaaaaataaa
     5641 caaaaaaagt gttgcttttt gtttgaatgc ttgatattat ataaacaaag aaatgatgaa
     5701 aacgtttttt cgaacatttt gcaaaatgtt ttctacttct acgtagcatt tctttctaaa
     5761 atttaggagg tggtcaaaat ggctattgtt gttggtgcag atctcaaagg tacgagatta
     5821 aaagatgttg tgaaaaattt tcttgtagaa gaaggttttg aggtaattga tgtgactaag
     5881 gacggacaag attttgttga tgttaccttg gcagttgcct ctgaagtaaa taaagatgag
     5941 caaaacttag gtattgtaat tgatgcttat ggagctggtc cattcatggt agcaactaaa
     6001 attaaaggta tggttgcagc agaagtttca gatgaacgct cagcttatat gacacgtgga
     6061 cataacaatt cacgaatgat tacggttggc gctgaaatcg tcggtgatga gcttgctaaa
     6121 aacatcgcta aaggtttcgt caatggtaaa tatgatggcg gacgtcatca aatccgagta
     6181 gatatgctta acaaaatgtg ttaagaaaag gagttaagga atgagaatcg caattggatg
     6241 tgaccacatt gtcacagatg aaaaaatggc agtatcagaa ttcttgaaat caaaaggaca
     6301 tgaggtcctt gatttcggga catatgacca cgcacgtact cactacccta tctatggtaa
     6361 aaaagtcggt gaagcagtag taagtggtca agcagacttg ggagtatgta tctgtggtac
     6421 aggtgtcggc attaacaatg cagtcaataa agttccaggt gttcgctcag cgttggttcg
     6481 tgatatgaca tcagctatat atgcaaaaga agagttaaat gcgaatgtta tcggtttcgg
     6541 tggaaagatt actggtggtc ttcttatgaa tgacatcatt gaagctttca ttgaagctga
     6601 gtacaaacca acagaagaaa ataaaaaatt gattgcaaaa atcgaacacg ttgaaacaca
     6661 taatgcacat caagcagatg aagatttctt tacagaattc cttgaaagat gggaccgtgg
     6721 tgactaccac gattaaggta taacaaatga ttctgacagt cacactaaat ccttcagtag
     6781 acatctctta tcctctagaa acattgaaaa ttgatacagt gaatagggtg aaagatgtta
     6841 gtaagacagc tggtggaaaa ggcttaaatg ttactagagt cttgtatgaa tctggtgata
     6901 aagttactgc tacaggtttc ttaggtggta aaattggtga atttattgaa agcgaattag
     6961 aacaatctcc tgttagtcca gctttttata aaatctcagg aaacactaga aactgtattg
     7021 ctatcttaca cgagggtaac caaacagaaa ttttggaaca aggaccaaca atttctcatg
     7081 aagaagcaga aggctttctt gaccactata gcaatcttat taaacaatca gaagttgtga
     7141 caatttcagg aagtttacct tcaggacttc ccaacgatta ttatgaaaaa cttatccaac
     7201 ttgcttcgga tgaaggagta gcagtcgttt tggattgttc aggtgcacct cttgaaacag
     7261 ttctaaaatc gtcggctaaa cctactgcca tcaaacctaa taatgaggaa ctttcacagt
     7321 tgcttggaaa agaagtaaca aaagatattg aagaactcaa agacgttctt aaagagtcac
     7381 tttttagtgg tatcgaatgg atagtggtct cattaggtcg aaacggtgcc tttgcaaaac
     7441 atggtgatgt tttctataag gtagatattc ctgatatccc agttgttaat ccagtcggat
     7501 caggggactc aacggtagct ggtattgcat cagctttaaa tagtaaaaaa agtgacgctg
     7561 acttattaaa acatgcgatg acattgggta tgttaaatgc tcaagaaaca atgacagggc
     7621 atgtgaatat gactaattac gaaacactaa actcacaaat tggagtaaaa gaggtataaa
     7681 aatggtactt acagaacaga aacgcaaatc tttggaaaaa atttcagata aaaacggtgt
     7741 tatctcagct ttggcatttg accaacgtgg tgctttgaaa cgtttgatgg cacagtatca
     7801 agatacagaa ccaactgtag ctcaaatgga agaactaaag gttttggttg ctgatgaact
     7861 tacaaaatac gcttcttcaa tgttgcttga ccctgaatat ggacttccag caacaaaagc
     7921 attggataaa gaagcaggtc ttcttcttgc ctatgaaaaa acaggttatg atacatcaag
     7981 tacaaaacgt ttgcctgact gcttggatgt ttggtctgca aaacgtatca aagagcaagg
     8041 cgctgatgcc gttaagttct tgctttacta tgatgtagac agctcagatg aacttaacca
     8101 acaaaaacaa gcttatattg aacgtgttgg ttctgaatgt gtcgctgaag atattccttt
     8161 cttcttggaa atccttgctt atgatgaaga aatctcagat gcaggttctg ttgaatatgc
     8221 aaaagtgaaa cctcgcaaag tcattgaagc gatgaaagtt ttctctgatc cacgctttaa
     8281 cattgatgtt cttaaagtag aagtaccagt taacgttaaa tatgtcgaag gttttgctga
     8341 tggtgaagta gtttacagta aagctgaagc tgctgacttc tttaaagcac aagaagaagc
     8401 gactaacctt ccatacatct acctaagtgc aggtgtatct gctaaattgt tccaagaaac
     8461 attgcaattt gctcacgact caggtgccaa gtttaatggt gtgctttgtg gacgtgctac
     8521 ttgggcagga tctgtagaac cttacatcaa agaaggtgaa aaagcagcac gtgaatggtt
     8581 gcgtactact ggattcgaaa atattgatga acttaacaaa gttcttgtta aaacagctag
     8641 tccatggact gataaagtat agtaaaaaca taaaacggag gatattgtta tgaacagaga
     8701 agagatgact ctcttagggt ttgaaattgt tgcttatgct ggagatgctc gctctaaact
     8761 tttagaagcg cttaaagcgg ctgaaaatgg tgatttcgct aaggcagata gtcttgtagt
     8821 agaagcagga agctgtattg cagaggctca caattctcag acagctatgt tggctcgaga
     8881 agcttctggg gaggaacttc catacagcgt taccatgatg catggtcagg accacttaat
     8941 gactactgtc ttattaaaag atgtgattca tcacctcatc gaactttata aaagaggagc
     9001 aaaataatgc ataaactcat tgaacttatt gagaaaggga aaccgttctt tgagaaaatc
     9061 tctcgaaaca tctatcttcg tgctattcgt gatggattta tcgctggtat gccagtcatc
     9121 cttttctcat ctatctttat ccttattgcc tatgtcccaa atgcttgggg attccactgg
     9181 tcaaaagaca ttgagacctt cttgatgact ccttatagct attcgatggg tatcctagca
     9241 ttctttgttg gtggtactac cgctaaagct ttgacggact caatgaaccg tgacctacct
     9301 gcgaccaatc agattaactt cttatctacg atgctagcgt ctatggtagg gttccttttg
     9361 atggcagctg agcctgcaaa agaaggaggc ttcttgacag ccttcacggg aacaaaaggt
     9421 cttttgacag catttattgc agcctttgtg acagttaatg tttacaaggt ctgtgtgaaa
     9481 aataatgtta ctattcgtat gcctgaagag gttccaccaa atatctctca agtatttaaa
     9541 gacttgattc cattcactgt agcggttgtt ctcctttatg gatttgaact cattgttaag
     9601 ggaactcttg gtgtaaccgt tgcagaatca atcggtaccc ttcttgctcc tctcttctca
     9661 gctgcagatg gttaccttgg aattacactt atctttggtg cttatgcctt cttctggttt
     9721 gttggtatcc acggtccttc aattgtcgaa ccagcaatcg ctgctatcac ttatgccaat
     9781 atcgatgcca acttgcatct tatccaagct ggacaacatg cagacaaagt tatcacttct
     9841 ggtactcaaa tgtttattgt taccatgggt ggaacaggag ctacattgat tgttccattc
     9901 ttgttcatgt ggatttgtaa atcagaacgt aaccgtgcca tcggacgtgc ctcagttgtt
     9961 ccaacattct ttggggttaa tgagccaatc ttatttggtg caccaatcgt attgaatccg
    10021 attttctttg taccgttcat tttcgctccg attgtcaacg tttggatctt taaattcttt
    10081 gttgacacat tgaacatgaa ctcattctct gccaaccttc catgggttac tcctggtccg
    10141 ttgggaattg tactcggtac taacttccaa gttctatcat ttatcttagc cggactcttg
    10201 gtagttgttg atactatcat ttattaccca tttgttaagg tatacgatga acaaattctt
    10261 gaagaagaac gttctggtaa aacaaacgat gctcttaaag aaaaagtagc tgcaaacttc
    10321 aatactgcta aagcagatgc cgttcttgga aaagcaggcg ttgctaaaga agatgtagca
    10381 gcaaacaata atatcacaaa agaaactaat gtccttgttc tttgtgcagg tggaggtaca
    10441 agtggcttgc ttgccaatgc cttgaacaaa gcagcagcag aatacaatgt tccagtcaaa
    10501 gcggcagcgg gtagttacgg tgctcaccgt gaaatgttgc cagagtttga cttggttatc
    10561 ttggctccac aggtcgcttc aaacttcgat gatatgaagg ctgaaacgga taaattgggc
    10621 attaaacttg caaaaactga aggagctcaa tatatcaaat tgacacgtga tggacaaggt
    10681 gctcttgcat ttgttcaaca acagtttgat taataaattt gtaaaagtta tttgagacga
    10741 gattattctt tcaattcctt ataatattgt taaccatatc attgcatttc atttccttgg
    10801 tttccggaac tcagaatatc gtctcaaatg ctttttttga aaggatttat agttatgact
    10861 aaaacacttc ctaaagattt tatttttggt ggtgctacag ctgcttatca agcagaaggt
    10921 gcgactcaca cggatggtaa aggcccagtt gcatgggata agtatcttga agacaattat
    10981 tggtacacag cagagcctgc aagtgatttt taccataaat atcctgttga tttagaatta
    11041 gctgaaaaat atggcgttaa tggtattcgt atttcaattg cctggtctcg tattttccca
    11101 actggctatg gcgaagtaaa tgaaaaaggt gttgaattct accataatct ttttgccgag
    11161 tgccacaaac gtcatgttga gccattcgtg actttgcacc actttgacac accagaagtt
    11221 cttcactcag atggagattt cttaaaccgc gaaaatatag aacactttgt agattatgcc
    11281 gctttctgtt ttgaagagtt cccagaagta aactactgga caactttcaa tgaaattggc
    11341 ccaattggag atggtcaata cttggttggt aaattccctc caggcattca atatgatctt
    11401 gccaaagtct tccaatcaca ccacaatatg atggtttctc atgcacgtgc tgtgaaattg
    11461 tataaagata aaggttataa aggcgaaatt ggtgtagtac atgcgttgcc aaccaaatac
    11521 ccttatgatc cagaaaatcc agcagatgtt cgtgccgctg agttagaaga tatcatccat
    11581 aacaaattta tcttggatgc aacctacctt ggacactatt cagataaaac gatggaaggg
    11641 gtcaaccaca tcctagctga aaatggtgga gaacttgatc ttcgtgatga agacttccaa
    11701 gcacttgaag cagctaaaga tttgaacgat ttccttggta tcaactacta catgagtgat
    11761 tggatgcaag cctttgatgg tgagactgaa atcattcaca atggtaaggg tgaaaaagga
    11821 agttctaagt accagattaa aggtgttggt cgccgaatcg caccagatta tgtgcctaga
    11881 acggattggg actggattat ttatcctgaa ggcttgtatg accaaatcat gcgagtgaaa
    11941 aatgattatc caaactacaa gaagatttac atcactgaaa acggtcttgg atacaaagat
    12001 gagtttgtag ataataccgt ttatgatgat ggtcgtattg attacgtgaa gaaacacttg
    12061 gaagttttat cagacgcaat tgcggatggt gcaaatgtta aaggttactt catctggtca
    12121 cttatggatg tcttctcatg gtcaaacgga tacgaaaaac gttatggact attctatgta
    12181 gactttgaca cgcaagaacg ctatcctaag aaatcagctc attggtataa gaaagtagca
    12241 gaaactcaag tgatagagta atattacttg aaaatgtaaa aaattgttat ccgatacttt
    12301 agaaaaagta caacacttat ttagaccagg ctttgaaaag gtatatattg ttagtttcga
    12361 agattgtcct atgattcctg agcttgaagc taccccgttg ctaaaatgtg gtaaatggta
    12421 tgtttcaact ggaaaagagt ggatttgcca ttctgattta gagttatcag catttgaatg
    12481 ggaattttta caatcactcg atgtagaaat acgtgagact attcacttcg aggtaaatta
    12541 tttgcctttc caataataaa aagaaaggaa ttagttgtga ctatcgaatt aaaaaacgag
    12601 tatcttactg ttcagtttaa aacacttggt ggtcaactga cttcgattaa agacaaggat
    12661 gggatagagt atctctggca agctgatcca aattattgga atggacaggc gcctattcta
    12721 tttcctatct gtggtagttt acgaaatgat tgggctattt atagacctca agaaagaccc
    12781 ttttttacag gtcttattcg tagacatggt tttgttcgaa aagaagaatt tattctggaa
    12841 gaagtcaatg ataatagcgt gacctttagt attaaaccaa atgctgagat gcttgataat
    12901 tacctatatc agtttgaact gagagttgtt tataccttga atggtaaatc aattagaacc
    12961 gaatttcagg taacgaactt ggaaatggaa aaaacaatgc cttactttat tggagcccat
    13021 ccagcattca atagtccttt agtagaaggt gaaaactatg aggattatta tattgaattt
    13081 agtgaggttg agtcttgctc gataccaaag agttttcctg agacaggatt attagatttg
    13141 caagatcgga cacctttttt ggaaaatcaa aaaattctcg atttggacta ctcacttttt
    13201 tcccatgatg ccattacact tgaccagctc aagtctcgta gcgtaacgct tcggtcaagg
    13261 aagtctggta aaggtcttcg agtagatttt gatgattttc cgaacttgat tctgtggtct
    13321 acaactaata aaagtccatt cattgctttg gaaccttgga gtggtctctc cacttcactt
    13381 gaagaaggta atttcttaga agataaaaga caggtaacga aaatttcacc tcaagaaact
    13441 tctagaaaaa tctatgatat tactatcctt aattaaaatt tgttattatt tgagtcttca
    13501 ataagatttg aatactctta ccttgtgatg atagattggt cttctccgtg ccagtcttac
    13561 atcgcaaggt ttttaagttt tttgtgggtc tatgtcaact gtagtgggtg acgaaaagct
    13621 aatatctaaa gacagtctgg tagtaaaaaa taagattatt tactacttct tgatttaaaa
    13681 taatagtgga gccattaatt tgtggcaaat ttaatttaat attttgcctc aaattagcca
    13741 cactttttat agaactaaga ggaaaaatga aattttcttc ctattaatat agttgtttga
    13801 aatcttaaat atagcgt
//
LOCUS       DACCCS010000072        13808 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.073, whole genome shotgun sequence.
ACCESSION   DACCCS010000072 DACCCS010000000
VERSION     DACCCS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.073"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IVK82_002327"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IVK82_002327"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP6393366.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="IVK82_002328"
     CDS             complement(2261..2512)
                     /locus_tag="IVK82_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP6393367.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="IVK82_002329"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="IVK82_002329"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP6393368.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="IVK82_002330"
     CDS             3462..3680
                     /locus_tag="IVK82_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393369.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="IVK82_002331"
     CDS             complement(3969..4481)
                     /locus_tag="IVK82_002331"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP6393370.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="IVK82_002332"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="IVK82_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP6393371.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7368)
                     /locus_tag="IVK82_002333"
     CDS             complement(6934..7368)
                     /locus_tag="IVK82_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP6393372.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="IVK82_002334"
     CDS             complement(7378..8166)
                     /locus_tag="IVK82_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6393373.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /locus_tag="IVK82_002335"
     CDS             complement(8163..9104)
                     /locus_tag="IVK82_002335"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP6393374.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="IVK82_002336"
     CDS             complement(9281..9868)
                     /locus_tag="IVK82_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP6393375.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="IVK82_002337"
     CDS             complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="IVK82_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP6393376.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11690..12700
                     /gene="gap"
                     /locus_tag="IVK82_002338"
     CDS             11690..12700
                     /gene="gap"
                     /locus_tag="IVK82_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP6393377.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12785..13720)
                     /locus_tag="IVK82_002339"
     CDS             complement(12785..13720)
                     /locus_tag="IVK82_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6393378.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3096 c         2435 g         4469 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgaaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata tgacagtaaa
    11701 agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt
    11761 ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta tgttagcaca
    11821 attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg caacagaaga
    11881 ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg caagcaaaat
    11941 cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt tttatacatc
    12001 tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta tttctgcccc
    12061 agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag atgaaaacga
    12121 caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg cttatttctt
    12181 aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct acacttccac
    12241 acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc
    12301 tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag ttatccctga
    12361 gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg gttcattaac
    12421 tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg aagcaatcaa
    12481 aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg tttctagcga
    12541 tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag taacaactgc
    12601 tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg gatttacttg
    12661 ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat caatcgataa
    12721 aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct acttttaaac
    12781 tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat ttttcaggat
    12841 ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct tttggtccct
    12901 tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct tcgtctccat
    12961 gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt gcttcaccga
    13021 aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc ggaaatgctg
    13081 gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa gaataaccgc
    13141 aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc atcataactg
    13201 ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca ttgttttcta
    13261 gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat ccaatgtaat
    13321 ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa cccatatcat
    13381 gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca tgagccacaa
    13441 gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat ttcagaccgt
    13501 cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat ttatgtgcat
    13561 ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag tctttctcag
    13621 aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca acaatcagag
    13681 ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca tctcatttct
    13741 cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc aatgttcatt
    13801 tttataac
//
LOCUS       DACCCS010000073        13145 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.074, whole genome shotgun sequence.
ACCESSION   DACCCS010000073 DACCCS010000000
VERSION     DACCCS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.074"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(77..149)
                     /locus_tag="IVK82_002340"
     tRNA            complement(77..149)
                     /locus_tag="IVK82_002340"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IVK82_002341"
     CDS             530..1099
                     /locus_tag="IVK82_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP6393379.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IVK82_002342"
     CDS             1086..1946
                     /locus_tag="IVK82_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6393380.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IVK82_002343"
     CDS             2136..2840
                     /locus_tag="IVK82_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6393381.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IVK82_002344"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IVK82_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP6393382.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IVK82_002345"
     CDS             4677..5993
                     /locus_tag="IVK82_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393383.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IVK82_002346"
     CDS             5994..6863
                     /locus_tag="IVK82_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393384.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IVK82_002347"
     CDS             6918..7727
                     /locus_tag="IVK82_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6393385.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IVK82_002348"
     CDS             complement(7830..8459)
                     /locus_tag="IVK82_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP6393386.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IVK82_002349"
     CDS             8629..9921
                     /locus_tag="IVK82_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6393387.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IVK82_002350"
     CDS             complement(9997..10659)
                     /locus_tag="IVK82_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP6393388.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IVK82_002351"
     CDS             10884..11168
                     /locus_tag="IVK82_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP6393389.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IVK82_002352"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IVK82_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP6393390.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2264 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccatt
//
LOCUS       DACCCS010000074        12997 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.075, whole genome shotgun sequence.
ACCESSION   DACCCS010000074 DACCCS010000000
VERSION     DACCCS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.075"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(95..1675)
                     /locus_tag="IVK82_002353"
     CDS             complement(95..1675)
                     /locus_tag="IVK82_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP6393391.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2076..3452)
                     /locus_tag="IVK82_002354"
     CDS             complement(2076..3452)
                     /locus_tag="IVK82_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP6393392.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3580..4698)
                     /locus_tag="IVK82_002355"
     CDS             complement(3580..4698)
                     /locus_tag="IVK82_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6393393.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKTATTS"
     gene            4840..5274
                     /locus_tag="IVK82_002356"
     CDS             4840..5274
                     /locus_tag="IVK82_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6393394.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5352..5885)
                     /locus_tag="IVK82_002357"
     CDS             complement(5352..5885)
                     /locus_tag="IVK82_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP6393395.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(5979..7157)
                     /gene="mutY"
                     /locus_tag="IVK82_002358"
     CDS             complement(5979..7157)
                     /gene="mutY"
                     /locus_tag="IVK82_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP6393396.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7150..7947)
                     /gene="recX"
                     /locus_tag="IVK82_002359"
     CDS             complement(7150..7947)
                     /gene="recX"
                     /locus_tag="IVK82_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP6393397.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8054..9436
                     /gene="rlmD"
                     /locus_tag="IVK82_002360"
     CDS             8054..9436
                     /gene="rlmD"
                     /locus_tag="IVK82_002360"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP6393398.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9610..10188)
                     /locus_tag="IVK82_002361"
     CDS             complement(9610..10188)
                     /locus_tag="IVK82_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP6393399.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10510..10980
                     /locus_tag="IVK82_002362"
     CDS             10510..10980
                     /locus_tag="IVK82_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP6393400.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11088..12314)
                     /locus_tag="IVK82_002363"
     CDS             complement(11088..12314)
                     /locus_tag="IVK82_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP6393401.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3845 a         2622 c         2188 g         4342 t
ORIGIN      
        1 gaaaatccgt cacggctcta aacaaaatat gagaggctgt ttttgtttgg ctattcttat
       61 tacttgatgc agggcagctt gtgtcaagac ttctttacat caaacttttt aattttacat
      121 tgggatattt atccgcaaac cagcgttctg caaattggtt ctcgaataag aacaacggtt
      181 ggtcgaaacg gtcacgagcc aagatattgc gacttgaact catcttctca tccaaatctt
      241 ctggatcgat ccagcgagcg attttgtgtc ccattggaga catgattact tcggcgttat
      301 actcatttaa catacgatat tggaatactt cgaattgtag ctgaccaaca gctccaatga
      361 tatattcttc cgttagatat gttttataaa gctgaatcgc tccttcttga actaattgat
      421 agattccttt gtggaaggat ttttgtttca tgacattttt tgcagtcact ttcataaaca
      481 gctctggagt aaatgaaggg agttcttcat aagcaacttt tagttttcct tcgaacaatg
      541 tatccccaat ctgataattg cctgtatcat atacaccgat gatatcgcct gctactgctt
      601 tttcgacgtt ttctctcgaa tctgccataa attgtgtgac attgcttaat ttcagttttt
      661 tatttgtacg ttccaaccag acatccattc ctcgttcgaa tgttccagaa cagattcgga
      721 caaaagcgat tcggtcacga tgggctggat tcatatttgc ttggatcttg aatacgaaac
      781 ctgaaaattc ttcctcataa ggacttacca tctgatcatc ttgtgttttg tgtccataag
      841 gagctggtgc caattcaacg aaagtctcta aaaaagtctg tacaccgaag ttagtcaatg
      901 cagaaccaaa aaatactggt gtctgatcgc cacgagcaat tttttcttga ttaaatgcat
      961 ctccagcctc tttgattagt tctacttctt ctaagacttg ttcatataag gaattttcag
     1021 ttagaggatg attttcggga agcagtccat cttcatccaa ttctactaat cgttcatcct
     1081 gataattttc cggacgataa aactctacac gattatggta gatatcataa agaccttcta
     1141 gccctttacc cataccgatt ggccaattca tcggataaga ttcgatatct aatagctctt
     1201 ctaattcttc taacaattcc aatggttcac gaccatcacg atctagtttg ttgataaaag
     1261 taaatatcgg gatgccacgt ttttttacaa cttggaataa tttctttgtc tgtgcttcga
     1321 tcccttttgc actatcgatt accatgaccg cactatccac ggccatcaaa gtacgataag
     1381 tatcttcaga aaaatcttca tgtcctggtg tatccaagat atttactcgt ttcccttgat
     1441 aatcaaactg cataacagaa ctagtaacag aaatcccacg ttgtttctcg atttccatcc
     1501 agtctgattt ggcaaaatta cctgtttttt tgccttttac ggtcccggct tgacggatag
     1561 ccccaccaaa caatagtaat tgttctgtaa tcgttgtttt cccagcatcc ggatgggaaa
     1621 tgatcgcaaa agttctgcga ttgtccactt gttgctttaa ttttggatta ttcataacct
     1681 aactccattt cttccattca attatttata ttttctctag gcacagattg tccgatctct
     1741 taaagaaaga caatttcata tagggctgtg acaaaagccg tgtagcagtc tctttttccg
     1801 agtaaacggc gttctagaga cagcctatca gcaataagcc cacccaagcc aaaaatacga
     1861 aaagctgttt ttgcttggct gctcttatta cttaatgcag gatagctttt tcacaacctt
     1921 tgtcaactac tacaactgaa tcagtatagc atggattcag cgaatgaaaa agccaccaaa
     1981 ccataagagt caaatatttt tttgtcagct gactaaaaga aaagacgtgt tgcttgttta
     2041 ttcaagcaac acgtctttca taaccaagtt tccaattatc ttcttggctc gtcatcttcg
     2101 aattcttttc tgaagtatct tcgttcttct tcaggttccg cttctgtttc ttccggatca
     2161 tgaaaaatga cacggatttt aaggattcga gaaccttcca tttcttcaga aaccagcgta
     2221 atattatcga catcaaacga taacttttcg ccttcatccg gaatcatgcc taatgctgta
     2281 atcaaataac cagccatcgt atctacatca ctcatatgca gatcactgtc aaaagcttca
     2341 ttgaactcat caatcagcat acgtccttgg atgatatatt cgtgttcatc gattttttca
     2401 taaagattct caacttcatc tgattcatca tcgatttctc cgacgatttc ttctaaaagg
     2461 tcttctagtg ttgctaaccc aacgacgccg ccatattcat ctaataaaat agccatctga
     2521 ttctgcgtct ttttcatttc atataacaga tcatcaataa agaccgtttc aggaacgaat
     2581 aaaggctcct gcatgatttt cttgatatct aaattatcaa agccgaattt atgggcagct
     2641 tttagtaaat tctttgtatg aaggattccg actactttat ctttgtcttc gttataaaca
     2701 gggattcttg aatagttttc ggataaaact tcattcacat tttcttccac agtgtcgttg
     2761 atatcaatca taaatgcatc cgttcgtggg accatgactt ctcgggcaac tttcgtatca
     2821 agagaaaaaa ccccttggag catttctaat tcttcattat tcaatacgcc ttcgttttca
     2881 agcatataac gcatttcatc tctggtcatt tttgaatctt catcatcaaa agtcatcggc
     2941 gtgatacgcg ccaataaact tgtggaagca gaaagcagcc agacgaaggg cttagctact
     3001 acaccaatca agcgtatcag ccctgaagtg aactttgcga cttcttctga tttatttaaa
     3061 gcaatacgtt tagggtacaa ttctccaaaa acaatcgata cataggtcaa aatagccagc
     3121 acaatgatat ttgcaatatt ttttgccgct gtgccgccac ctaataccgg agctaaccgt
     3181 tccgataaag tattagctag tgaagcacca gacaaaatgt tgaccaaagt gattcctact
     3241 tggattgtcg ataggaaatt atttggattc tgcaatattt ttaaaagctt ttgagatttc
     3301 acatctcctt catcagcttt ttgctcgatt cgattcttat tgatcgaaac aacagcaatt
     3361 tcggaagctg ctaaaaaagc attgatcaat gtcaaaacga tcaataataa aatctgcgct
     3421 aacagcgact gactctcagg gtcagcattc ataatcgcat tctctcctta taaaattaat
     3481 ttttagttta cgatagaaaa aaacgtggca ctatagccac gttaaaatgg tatcacaaag
     3541 caattttctt tgcaataaaa acaagtgtac taaaactacc tacgaggtcg tagcagtttt
     3601 cttcttgttt tcattttgct tgtcttcttt gacgattttg attatatagg aaatgatcgt
     3661 cttgcagatt gcgtaaaaag gaacacctaa gaagatacca agcaatccgg caatatttcc
     3721 agctactaga agaacgataa tgatcgttaa tggatggatt ttcagtgatt tcccaattac
     3781 attaggataa atgatattcc catccaactg ttggacgatt agcacaacca cacaacaaag
     3841 taatgctttt accggatcat tgaagactgt tactagaaat gcaggtgcta gtcctagata
     3901 aggtcctaaa tatggaatta ggtttgtcaa tcccgcaatg accccaaata agaaagcata
     3961 atccacacca attaccatat atcctaaaaa tgtgaaggta ccaacgaata aacattcgat
     4021 tgcttgtccg ctaatgtaat tagccaatgt tttattcagt tgccccaata actccacaat
     4081 ttggtttctt cttttttctg ggaaaaactg ttggatgttt ggcactagtt tttctccgtc
     4141 tttcaacata tagaacaaga tgaatggtgc tgtaatgatt actacggttg ccgaagcgat
     4201 cgttccaacg atcgaaccta aactattgga tactccacta aggaattgct gaatgattgt
     4261 cccgtaagaa atgtctaatt gctcaaaata tttattgata tctacttcct tgaacaaagg
     4321 caattcggct acctctttta accagctttc tacctgtttg atgaaatcag gcatattaga
     4381 tgccaatgag gaaatttgct gtactagact tgggatgaca ctaagtaaaa tccaaataat
     4441 tgctgcaact aaaaggagca gcacaattgc gacagcccat attcttttga tttttgcttt
     4501 catcaacagc ttgacaatag gattcaataa atagtaaaga aagcctgcaa tcagtacagg
     4561 tgcaaataac gtggaaaaaa atgtcccgat cggttcgaaa ataaagttta tttctgatga
     4621 aatcatgatc aatgcagcta tgatcaataa ttcaagcgac caaaacatta gtttagactg
     4681 tctaagtttt tcaaacaata gatttcctcc tttctttctt actagtatat attatattcg
     4741 ttaaaaattc ttttattctt tttttatttt tgaaaacttg ccatactttt tatttgtttt
     4801 attttccagt atactatacg aaaggaaggt ggctagacca tgaaagttgt acaaacaaaa
     4861 gatacaatga gcaatattta cttagatgct gtacggatca ggcatcaagt ttttgtagtc
     4921 gaacagggtg tgcctttgtc tcgagaaata gacaaagatg aagcccattg tatccacttc
     4981 gtcctgtact cagataaaaa agaaccgcaa ggaactgtac gtcttttgcc tttagaaaac
     5041 ggaaaaatga aattacagcg gatggcgatt ctttctgaat acagacacca agggttagga
     5101 aaaatattga ttgaagaagc agaaaacttt gctaagaatc aaggctataa tacgatttta
     5161 ttaggggcac agtccacagc tgaaactttt tacgaaaaac taggatatac agcttacggc
     5221 gatccttttg aagatgctgg catgcctcat atttatatga aaaagacttt ataaaaattt
     5281 atttcgaaaa aagcaaaaaa cagcaagctt ttgagaaaaa tctctcactg gcttactgtt
     5341 cttttttgtg tttacttacg cgacaattgc ttgtaacggt tataccaaat atcaatatag
     5401 ccttccgaga acgggccatc tccattattg atccagtcaa ccagaatctt cacattttct
     5461 tttaggatga agtcaatatc gttcgggtaa ttcatcattt tgctgtgcat ctcatattca
     5521 tcgacatcca atagtcgttt ctcgccgtca ggaaatacct tgatatccaa gtcatagtcg
     5581 atatatttca aggcctcatc gtccagcaaa aaaggtgaag ctagattaca ataataggaa
     5641 accccctttt ctctgatcat tgctattaca ttgaaccagt attttttatg aaaataaaca
     5701 atcgctggtt cgcgcgttac ccatcggcgt ccatcagatt cagtgactaa tgtgtgatca
     5761 ttcacgccga tcatagagtg ttcgcttgtt tttaatacca tggtatctcg ccacgttcga
     5821 tgcaatttgc cgtcgtgctt gtaactttgg atcgtcacaa actctccttc ttttggaact
     5881 cccatagcaa cccttcctta agcagcattt tgtgctttgt gtttgggtgt tcattacacc
     5941 gacatcataa ttattatatc aataaaaatg agctttgcct agaaaggatt cctattgttt
     6001 cgatttttct ctaactgatc taccagtttc atttggacct taggaaaaac gaccgaatca
     6061 aatgcatcag gcttcaacca cttcgaagtt ttgttctcgg taaattcttg ttctgctccc
     6121 tcggttgccc gtccgtaaaa aaggagaaca tgccatttca gatgactaaa aatatgagta
     6181 acttctccta aatgtcgtgt ctgccaaaca aagagttcct gcttttcaaa tgggagattt
     6241 tgctcttctt ctgctactag gtcatcaaac agatcgattt ctggctttgt ttcccattct
     6301 tttttcaatc gctcatactc ctcttgtgtc acttccatca ttggaaatgt ccacatattc
     6361 gccagcaatt tttggctatc tctttcttca aaataatacg ctccagaatg attttgaaga
     6421 gctgcactga tataataaac gtcttttggc tttgcttttt ttgtttttac aggaaaactc
     6481 gtttggattc ctcgtttgtt agctaaacaa aaggcttgga tcggacaagt ctcacacttt
     6541 ggagaagttg gtgtacagat agctgaacct agatccatca ttgcttggtt gaattcacct
     6601 ggatgctttt catcaatgat tttccgcatc gcttcatcaa agattttcct gctagaagct
     6661 tttgcgatgt ctgcttcaat gcaaaacaat ctgctgacta ctcgcatcac gttgccgtca
     6721 actgcaggtt ctggaagtcc aaatgcgata cttgcaatcg cgcctgtcgt atatggtcct
     6781 attcccttca atgagctaat ttcttcaggc gtttgcggca tttccccatc aaattctgac
     6841 atgatctgtt tagcagcagc ttggatgttc cgggctcttg agtaataacc aaggccctcc
     6901 catgctttta aaagcttttc ttctggagca tttgctagct cttcaattgt aggaaaccat
     6961 tccatgaaac gataaaaata atcgatcact gtgtctactc tggtttgctg aagcatgatc
     7021 tcagagatcc agatacgata aggatcacga ttatagcgcc aaggcaggtt tcttttttct
     7081 tgttcatacc attgaatgaa ctgatcttga aattcttttg tttctttatc tgtccatttt
     7141 tcccattctt tattcacttt cctcttcctt ttccgtaatg aatcgctgga tcatatccaa
     7201 atcaaatcct ttttgatata agctttgctt tacttttaga tttctttttt taggttcaaa
     7261 ccttaaattt cttcgccata gtttatctcc ttgtttgact aaataatcat attctatttc
     7321 ttgatctgct tcttgcggaa gatcagctat cgcttgttgg atcacttctt gagaaaagcc
     7381 tttcgtcatc aatgtttgtt ggatttttct caataattct ttttggcttt ttccgtgatt
     7441 ttttccaaac gctttttcgg ctgtctgatg tgcattttga acttggtctt caaatgtata
     7501 ttggctgagc gcttgttcaa tgatttcagg tttcaaacct ttttgctgca tctgctgcgc
     7561 taatttcttt ggtcctttgt cacttaagcg catattcgtg cgcacataac tttccccata
     7621 gaccaaatca tcgacaaggt ccaattcttt taagcgggct acgattttat ttcgatcttc
     7681 ttgaaggatt tccttttctt ttaagtatgt tcggatttct ttttccgatc gcaactgata
     7741 acttaagtaa ttcattgcta ggtgaaaacc gaaatcagct ccgctactct tcttgatctc
     7801 ttggatagtt tcttcgtcta attcccgtcc ttttaataat cgatagcgta ctaatacatc
     7861 ttcagatact tgtaaagctt ctccttcaga taaatctacg cgatagaaag ggcctttgcc
     7921 tttactgatt ctcgtaacgg ttaacataaa catcctcctc gaacatatat tctctatcat
     7981 accacagaaa atccccgtaa tcaggtatta tatgttagaa taaaccacag aaaaaagaaa
     8041 aagggagata cttatggcag aaacaattgt caaaactgga caaacggttc ctttgaaaat
     8101 caagcgttta ggcataaatg gtgaaggaat cggttatttc aaacggttga tcatatttgt
     8161 tccatatgca ctaccaaaag aagaagtact agtgaaaatc acgaaagcaa ctcctcgcta
     8221 tgcagaagga gaattaataa aagtcaaaaa aacaagcaaa gatcgcgtag tggcaccatg
     8281 tcctgtttac tatgaatgcg gtggctgtca attacaacac ttagcttatc aagctcaatt
     8341 ggattttaaa aaggatctgc ttttgcaagc attggaaaaa ttcaaaccag caggctttcg
     8401 atcttatcaa cttttgccga cgatcgggat ggatgagcct tggcattata gaaacaaagc
     8461 acaatttcag ttacgaaaaa ataagcagac tcaaaaaatt gaagctggtt tatacgaagc
     8521 aaactctcat cagttagtcc ctataacaga ctgcttggtt caagaacctc gaacacaaaa
     8581 agtaatgaat actgttgttc aactattgaa taaatttgat gcacccatct atgatgaacg
     8641 gacaaacagt ggaatcttcc gcacaatcat ggttagaatc ggtgttaaaa ctggggagtt
     8701 acaagttgtt tttattactc gcagtaaaaa attcccgcaa aaaaacgcaa tgatccgtga
     8761 gatcaatgaa cgactgcccg aagttgtttc aatcatgcaa aatgtccagg cacaaaaaac
     8821 gtctctcgtg atgggggatg aaacgatcca cttatgggga aaagaagcca ttgaagaaca
     8881 aatcaatgaa gtgactttcg atctttctcc tcgtgccttc tttcaactca atcctcagca
     8941 gaccgaattg ctttatggcg aaggaatcaa agcattagat gtccagccta atgaaacgat
     9001 cgtggatgct tattgcggag ttgggacgat cggcttaagt gtagcaaaac aagccaaaga
     9061 agtacgggga atggataccg ttcctcaagc aatcgctgat gcaaaaaaaa atgcggaaag
     9121 attaggatat cacaacactc gatatgaagt cgggacagca gagcaagttc tgcctaaatg
     9181 gttgaatgaa ggttttcagc ctgatggtat catcgtagat ccaccacgta cgggtttgga
     9241 taacgcattg atccaatcat tattgaaata ccctccaaaa aaacttgttt atatctcctg
     9301 taatgtttct acacttgcca gagatctggt taatttaagt aaaaagttca agattgaata
     9361 tttacagtcc gtcgacatgt ttcctcagac tgcacgttgt gaagttgtcg tgaagatgac
     9421 gagactcgct gactaaatga aagaggttgt gaaaaagtct gccctgtatc aagtaataag
     9481 aacagccaaa caaaaatagc tcttcatgtt ttatgaatac tgtacttgtg accgaagagt
     9541 taacaggagg acactattta tttaaaaaaa gggttgtgac atagtttttg tcacagccct
     9601 ttttgattat tattggatat tcacgtctat cacttgataa aatgcatttg tcgtatcgct
     9661 gatattccat actgctaaaa tcacattata tccttttcga tcctgcggaa gtgtgatcgt
     9721 ttggttgact gtttttggtg gaacagttgc tttgtcgtct atttttgtga tcaattcaaa
     9781 atcactaaat ttcaaaggtt gattaggatt ccaagttggt ttggtaatgt agtaatccca
     9841 agtacttgtc cgatgttgtg ctgttaaatt ccaagtaata tttaatgttc ctggattaac
     9901 catgttttta tgccatctgt tagatgtctg ttcatctaaa ggctcaaagc cgctgactcc
     9961 tgcactagca atttttccat caatgaatgt atttttggta gcttcgatac tttgcggttc
    10021 ccattgtgct cttccgacat tttgatttaa gtttcctgct gctgatgttc ctaggtatgc
    10081 acggcttcct ggagaagtaa tataaccatg cgcaaaacct ggaatgctaa aaccgaacaa
    10141 ccctaaacta actaacccaa taaacaataa cgtactaatc tttttcatgt agaaacctcc
    10201 ttcatttgat aaccacattg taaacgcttt attgtattaa cacaaacatt atgcttttat
    10261 aattttatgt tgaagtaaaa aaggtgaaaa aagtaagatg cagattataa cagaagtaga
    10321 cgagaaaagt acaaatagaa aacatgagaa tcaaatcata tgatcttttt caaaaacatc
    10381 attcatataa acaaatgttc aactttttaa taacaacaat tatcaatttt tttcttactt
    10441 cacaaactaa atacaggtct tgctgtgagc cttcatggta aactaatatt atagaaagga
    10501 gttagtaaaa tgaagatgat aaaagatatt cgtttttggg tttgtgttat aggaattgtc
    10561 atattaggct ttttatcagg gttgcttagc ggaaatcccg gaaaatatta ctattctttg
    10621 caacttccac catttgcacc acctagctgg atatttgggc cgatgtggac cttactgtat
    10681 atattgatgg gtatttctct ttatttgtta cttaatcaca acaacaaaaa acagcgaaac
    10741 aatcttgtcg gtctttttgt gattcagttc atttttaatt ttatatggtc tgctctattt
    10801 tttaatttaa gaaatatttt cattgcagca atcgatatta cacttttagt tattttcttg
    10861 tcagtgctta tgtatcagtt gtggctgcat catcgcctgg caatgtggct catgattcct
    10921 tattatttat gggtactatt cgcaactcta ttgaattatt caatttattt tttaaactaa
    10981 gcgctaaaaa gagacgtcct gtaatttaac acatagaaaa agtgccacct aagttagatt
    11041 ttttgtctaa tttaggtggc actctttatt aaccaaacta tttttttcta gtacccactt
    11101 gttgttccat actcattatt ctggtcataa ccataaccat tttgttcctg tccagcgtaa
    11161 ggatcataac tttgagtatc ttgacttggt gtatagtact gacttgtatc ttctgtttgc
    11221 tgataatagg tattcggatc ttggtactga tcatatgtgt tttctgaata ggaagaataa
    11281 tcggtgtacc cttcactatt cgatccatca ccgtttgtcg aatcgccatc ttccgaatca
    11341 tcaaagaaca tcattgaagc attctcattt ttcaaattag gtaatgtatc gcttgttttt
    11401 atatttaatt gtttcttcag ttcattttgg atcgaaagaa gatcattttt tcctaaaatt
    11461 tgatagtaga tatcattgat catttgtcct tcgccttgca actggtcttg tttgattgtt
    11521 tcaaacgccg gggtgtagtt cgtcgctaca tcaagcatgt catcccaatc taagtccgtc
    11581 gtcacattgt tctcaactgc ttttaaaatt tttctataat tgcttacgcc atctaaactt
    11641 aaaactttat tgacgatttt cgtcactact tcccgctgac gacgttgtcg accgatatcg
    11701 ccttctggat cttcgtgtcg cattcgagaa taggctaaac ctttttctcc attcaatgtt
    11761 tgttcaccta ctggtacatg gacaccatcc aaagtaaagt caattttatt attcactttg
    11821 atgccaccga ccgcattgat caaatcttcc atgccatcca aattgatcgt tacataatga
    11881 tcaataggaa tatcgagcag ttgctcaatt gaatccatgg ccatttcgac accaccaaat
    11941 gcgtaggcat gatttaattt atctaccgtt ccataaccta cgatattcgt ataaatatct
    12001 cggtccaaac tgatgatcgt tgattttttt tgtttcggat taactgttac taccatcatc
    12061 gtatcagaac gtccttgttc cgttcttcct aatccaccgg tatctaaacc taatagcaaa
    12121 accgaaaaag gttctttgtc atcaatgctt actctctcag aacgtttgga ggatattcga
    12181 ttagagtttt tagaaatttt gtttactgtt tggctcgctt ctcccataaa tctgattccg
    12241 tatgctgata atccggcaat cagcaaaacg aataccccta acaaggttaa tagaactttt
    12301 tgccatcgct tcataataca cctctgtttc acattttcta aaatattgtc tcatataact
    12361 gttcaatcag caaattcact ttttaatcat aagaaaactt taaaaaacat ttttgtgtaa
    12421 cgattattat tttttaaaat ttctcaaaat tcttaactat cataccatac cgtgaaaaag
    12481 atcgaaagac cgaatacacg cagttcgcaa tcgttttatc tttttttatg tattttttcc
    12541 agaaaaagcg aacaacaagg tattttccaa ataatcgtgc agtaaaatga tcgcacatac
    12601 ctttcccatc tttttaacag tctttgccta ccgttcagat tatcattcga tttcacgaca
    12661 atttcttcct atgactggtc cttttgttaa acccatcttc tattcgtttt atttccagct
    12721 cactgcatgg ctcttaaaat gactcgaact agtgattgca ttaccatcat cttttaatgg
    12781 atagatatgc tcctcttcaa agtaaaacga ttttctttta aagtaataga attgacatgg
    12841 taatgagtat gtatactagt ggtttcacaa atggtctttt attcaacatc caaaaactga
    12901 aatttcttta aaatccatta ctaacgaaga aaatgttcct tgcttgataa ttagttagga
    12961 catgtcaagt gaacaaatgc atttacttag ccaaaaa
//
LOCUS       DACCCS010000075        12901 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.076, whole genome shotgun sequence.
ACCESSION   DACCCS010000075 DACCCS010000000
VERSION     DACCCS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.076"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(117..1565)
                     /gene="cls"
                     /locus_tag="IVK82_002364"
     CDS             complement(117..1565)
                     /gene="cls"
                     /locus_tag="IVK82_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP6393402.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1717..2610)
                     /locus_tag="IVK82_002365"
     CDS             complement(1717..2610)
                     /locus_tag="IVK82_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6393403.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2594..3280)
                     /gene="ftsE"
                     /locus_tag="IVK82_002366"
     CDS             complement(2594..3280)
                     /gene="ftsE"
                     /locus_tag="IVK82_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP6393404.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3385..4486)
                     /gene="prfB"
                     /locus_tag="IVK82_002367"
     CDS             complement(join(3385..4422,4424..4486))
                     /gene="prfB"
                     /locus_tag="IVK82_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP6393405.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4612..7146)
                     /gene="secA"
                     /locus_tag="IVK82_002368"
     CDS             complement(4612..7146)
                     /gene="secA"
                     /locus_tag="IVK82_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP6393406.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(7368..7919)
                     /gene="raiA"
                     /locus_tag="IVK82_002369"
     CDS             complement(7368..7919)
                     /gene="raiA"
                     /locus_tag="IVK82_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP6393407.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(8047..8766)
                     /locus_tag="IVK82_002370"
     CDS             complement(8047..8766)
                     /locus_tag="IVK82_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP6393408.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(8763..10076)
                     /locus_tag="IVK82_002371"
     CDS             complement(8763..10076)
                     /locus_tag="IVK82_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP6393409.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(10157..10477)
                     /locus_tag="IVK82_002372"
     CDS             complement(10157..10477)
                     /locus_tag="IVK82_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6393410.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(10585..10872)
                     /locus_tag="IVK82_002373"
     CDS             complement(10585..10872)
                     /locus_tag="IVK82_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393411.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            10999..11643
                     /locus_tag="IVK82_002374"
     CDS             10999..11643
                     /locus_tag="IVK82_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP6393412.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(11702..12409)
                     /locus_tag="IVK82_002375"
     CDS             complement(11702..12409)
                     /locus_tag="IVK82_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6393413.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
BASE COUNT         3565 a         2798 c         2243 g         4295 t
ORIGIN      
        1 ttaatttcgc tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg
       61 ttagaaaagg acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa
      121 aggagcggcg aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt
      181 tcatacattt cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt
      241 acacgtgaag ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat
      301 gaacgaacat cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc
      361 tttgcatgaa taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct
      421 gcagcgaaag aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg
      481 atatgaactt ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc
      541 ggtatgtaat aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa
      601 taagtcattt tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct
      661 atatcaccag tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta
      721 tgctgagaat tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa
      781 atacgcaaat gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt
      841 ccaacattga acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga
      901 ttgcgataat tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact
      961 tctccgcctg cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat
     1021 gcatcaagaa ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga
     1081 acttcttcac ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct
     1141 gcctgcttga tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg
     1201 atttcatttt tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt
     1261 ttcacatcta cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg
     1321 tcttcttcaa tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc
     1381 tctttggaaa tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac
     1441 atcaaaacga gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg
     1501 ataaaggaaa gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt
     1561 ttcacggctt cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa
     1621 agggaaaaaa gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa
     1681 actgtccaac tttctcattt tatttatatt gtggtattag aatttcaaaa aacgtctcat
     1741 agaaatgatc gagccaagtg aaccaatgat cacaccaatg ccgaccatta aaagattgat
     1801 tttccaaacg atattttgtg gacgaatcaa tgaataatgg gaacgtaata atgatggatt
     1861 gatcaattgg aaaaaccatg cataaccaaa tgtgatgatc aaaatcggaa gaatcgcacc
     1921 gatcagaccg atccagccac cttcaaggaa aaacggccag cgaatatatc cattttttgc
     1981 accgactaaa cgcatgattt gaatttcacg ctgtctagac aaaatcgtga tccgaatcgt
     2041 gttcgaaatg agaaacatcg ctacgaacag cagcaatgcc gctgcaacta atccccatgt
     2101 ccgaactgca ccagcgattt ggaaaatccg atctgaggaa gctccaccgt aatcagaacg
     2161 agaaacattc ttcaaatctg ctgcttcttc tgctacttgt tttgtataag aaggatcagt
     2221 agcacttact acatacacat catataacgg gttattgtca ccttcaaaca atttccagga
     2281 atcacccatt tcgttttgga tttttttcaa ttcctgttct ttgcttgaaa attcaacttt
     2341 tttaacatga tctaagtcat tcaattcttt ttctagcttt ttcatctctt cgtcttttgt
     2401 accgatatct acgaatacag atacatcaac gtttccttct atatcctgag ctaatttagt
     2461 tgcattcatg ataactgcca tgaatacacc gactaaagtc aacgtgatcg ttaccgcact
     2521 gactgaagca actgtcatcc agccgttacg tttcaaactc ttgatacttt ctaataaatg
     2581 gcggaaaaag gttctaatca tcgtatccat attccccttc cgcttgatct cgaataatgc
     2641 ggccgttttc gatagcaatt actcggtgcc gaatcgtatt tacgatcgta ctgttatgtg
     2701 tagccatcac gacagtagtt ccttgggcat tgatccgatc taataatttc atgatttccc
     2761 atgagttttc tggatccaga tttcctgtcg gttcatccgc gataagtact ttaggcgtat
     2821 tcacgattgc ccgagcaata gagacacgtt gctgttcccc accggataat tcgctaggaa
     2881 agactcgaac tttgtgtttc aaaccaacaa ggtccagcac ttccattact cgttttttga
     2941 tatctcgagg ttttctgccg ataacttgca tagcgtacgc aacattttca tatactgttt
     3001 ttcttggtaa taatttatag tcttggaaca ccacgccgat ttctctgcgt agataaggaa
     3061 cttcgcggtt tttgattgct attagatcat gacctgcgac gttcaaagtt ccttttgaag
     3121 ctttttcttc tcgatacatc aatttgatga atgtagattt accggctcca gacggaccaa
     3181 ccacatagac aaactctcct tgttcgatga caatcgagag attgcggata gcggtcgtac
     3241 catttgagta cttcttcatt acatccttca tttcaatcat ggcgttctct ccatatcttt
     3301 ttcagtcttt agctattata gcatggcaat gtttcaacat gcttgcaaaa tcttacaatt
     3361 ttatttcaat ttaacaatga gttcttattg tagatgcatt tttaaatagc cgtcaataaa
     3421 taaatcaagg tcaccgtcca taactgcttg gacatttcct gtctctacgt ttgtccgatg
     3481 gtcttttacc atagaatagg gatggaaaac ataagaacgg atctgtgaac cccagccgat
     3541 ttctttttgt tctcctcgaa gtgcagctgc ttcttgttct ttcttttcca cttctaattg
     3601 ataaagctta gcttttaaca tccccatcgc ttgctcacgg tttttcaatt gtgagcgttg
     3661 tgcctgacta gctactactg ttcctgtagg caagtgagtg atccgtacag cagattctgt
     3721 tttattgatg tgctgaccac ctgcaccact tgctcggtac gtatcgattt tcaaatcatc
     3781 tggattgatt tctatatcga tcgtttcatc aagctctggc atcacatcta ccgaacaaaa
     3841 agaagtatga cgtcgtttcg ctgagtcaaa aggcgaaatc cgtacaaggc gatgaacacc
     3901 tttttctgat tttagataac caaaagcgtt atgtccttta atcagcaggg tcacactctt
     3961 gatccctgct tcatcacctg cttgataatc tagtgtttct acttgatagc catgctgctc
     4021 tgcccaacgt gtatacatac gtaaaagcat gctgccccag tcttgggact ccgttccgcc
     4081 agcccctgga tgcaattcga cgatcgcatt gtttcgatca taaggtccgt ctaacagcat
     4141 agataattca tacgtcttca ttttttcatc taatgcattg atacgtgttt ctgcttcttc
     4201 ttgcattgct gcatcaggtt cttcttgcag catctccacc atcaattcga tttcttctag
     4261 ttcttcggca agagaatgga attggtcata ggtttcttta ttagcgttat tttcgctgat
     4321 cactttttgt gcggcttcgc tgtcatccca aaaaccaggt tcagccattt ttgcttctgc
     4381 ttccgcgatg tcttcttcca gctgatctaa gtcaaagaga cctcctgaaa cttgtgacct
     4441 tttcctgcat ttctgtcagg agatttcgaa tttcggatat ttccatgttt tttccttctt
     4501 tcactatact tttttcatta tatctagtct aacagaatca gcaaataaca gaaagaaggt
     4561 gagacgaatt cttaaaaaaa gaatttgtct caccaatcct gctgtcagaa attagtttgc
     4621 attttttcca tgacagttct tatatttttt cccgcttcca caaggacaag gatcatttct
     4681 tccgaccttg ttcacatgga ttggctgttt tttcgcgcta gtattactat tttctttggc
     4741 atctccgtct tctgagtgag aagctgttcc ttgagaaacc tgttcacgct gtacgttttg
     4801 acggatctca gctttcataa agattcgagt gacttcatac tcgatggcgc ctaccatatc
     4861 ttcaaacatc ttatagcctt ctgtttgata ttcgactaat ggattgttct gtccataagc
     4921 acgtaagccg attgactgac gtaattgatc cattgcatca atatggtctg tccatttaga
     4981 gtccacaaca cgcaagatca cgactttttc aaattccaac agttgctctg gaccatttag
     5041 ttgagccgct ttggtattaa atacttcttg tgctcgttcg tttagatact ctttgatctc
     5101 ctgagatgtt tttccttcga tatctgcaag agtaatacta tcttcatgga caagagtact
     5161 gccggcaaaa tcgatgatac tttccaaatt ccaattttct ttctctaatt gtgtatgcgc
     5221 atctactaca cgactgattg tccgtttgac catgttcatt agtggcttgg ataagctttt
     5281 ttcttccatg attacttctt gtcgctgttt atagattact tcacgttgtt cacgcatcac
     5341 atcatcgtat tgtaagacgt ttttacgtgt gtcatagtta tttccttcta cacgtttttg
     5401 tgcagattct acttgtttcg tcagcatctt gctttggata acagcatctt cctcaccgat
     5461 cttcatccga tctaagaaag ctttgattcg ttctgaaccg aatcgtttca tcaaatcatc
     5521 ttccaatgac aagtagaatt gtgacatacc agggtctcct tgacgtcctg cacgtcctcg
     5581 aagctggtta tcgatacgac gagattcatg tcgttctgtc ccgataactg ctagaccacc
     5641 aagttctctt accccaagtc ctaacttgat atctgttccc cgaccagcca tgttggtagc
     5701 aatcgtcaca gcaccttttt gaccagcatt catgatgatc tctgcttctt taaagtgatt
     5761 tttcgcattc agcacttcat gaggaatacg ttcttgattc agcatatctg ataataactc
     5821 agatgtttca accgcaacag ttccgactaa cacaggttga cctttgcggt agcgatcttt
     5881 gatatcttgc acgacagcgt ggaacttgct ttgtaatgtt gggtaaagta aatctggacg
     5941 atcatcacgg ataaccggac gatttgtcgg aatctggtat acttggatgt tataaatttc
     6001 tctgaattct tcttcttctg ttttagctgt accagtcatt cccgcaagtt tcttgtacat
     6061 acggaaatag ttctggaaag tgatcgtcgc catggttttc gtttcgtctt cgatctctac
     6121 gccttcttta gcttcgattg cttggtgaag tccatctgaa taacggcgtc catccatgat
     6181 ccgaccagtg aactggtcaa caatcagtac ttttccgtct tggacaacat aatcgatatc
     6241 taaaagcatg atatagttgg ctctcaatgc ttgatccaag tgatgggtca atgcagtatt
     6301 ttcaatgtcg tataagttat ccaaaccgaa tgtttcttct gctttttcga tccctgcttc
     6361 ggttaaactg attgtttttg attgtacatc gattttataa tcttcttctt ctttcaaacg
     6421 tttcacgaag ttgtctgctc ttgtatataa agctgtggat ttttctgctt gccctgaaat
     6481 gatcagcggc gttctcgctt catcaatcaa gattgagtca acttcatcga caatggcata
     6541 attcagcgga cgttgtacca tttgatggcg gtaaacgacc atattgtctc tcaagtagtc
     6601 aaatcctaat tcattattcg tactataagt gatatcacaa ttataagcct cgcgtttttc
     6661 atccgaagat tttgagttga tattcaaccc cactgttaat cctaagaaat tataaagttc
     6721 gcccatttcc gtcgagtcac gggttgctaa atattcattg acggtaacta cgtgtacccc
     6781 ttcgccagtc agagcattta gatatactgg catcgttgcg gtcaaggttt taccttcacc
     6841 agtacgcatt tcagggatat ttccatcgtg aagcacgatt ccacccatca attgtacgtg
     6901 gtatggatat aaccccaata cacgttttgc tgcttctcgg acgactgcaa aagcttccgg
     6961 aagtaattga tctaatgttt ctcctttttg gtatctgccc ttgaattcag cagtttttgc
     7021 ctgtaattgt tcatcagtta atgctgccat ttggtcagcg tatgcttcaa ctttttttgc
     7081 aatgctatct aaacgtttta gttctttctt atcgttttcg ataagtgaac gaatcaaatt
     7141 ggccatgcgt tgtgtaatct ccttttcctt ctgtgtatta tccagactat gtagacttta
     7201 cttttcattt tatcattagt tatagataat tgaaatatct taagcgtatt aaagtaagaa
     7261 cttcataatt tattcatatt tcatctaaaa gaggaggaaa ctcaaaaaaa cttgcggcag
     7321 ctgtcagtag ctgctgcaag ttttcgttgt tatttaagta aggctgatca gtctgtttca
     7381 atcagtccgt attttccatc tttacggcgg taaacgatac ttgtaccatt tgtttctgca
     7441 tcttcaaaaa taaagaagtt atgtcccagc atgttcattt gtaagactgc ttcttcacta
     7501 tccattggtt tcaatgaaag gcgtttcgta cgtacgatat ctaattcaga atcattttcg
     7561 ttttcgtcct cttcattgaa taaagcagca gcatctgcag tattcatgcc cgtttcacgg
     7621 gatttacgat taatttttgt tttgaatttg cggatttggc gttccaattt atcaacaacc
     7681 aaatcgatac ttgcgtataa atctggagaa gtttcttcgg cacgtaatac aagataaggt
     7741 aatgggatcg ttacttccac ttttgctgtt ttttctgtat aaacttttaa gttcacatgc
     7801 gccgttgcat cgggtgcgtc actgaaatag cgttctagtt tgcctacttt tttctcaacg
     7861 tagtcgcgaa tcgcttcagt tacttcgata ttttcgccgc gtacattata tctaaacata
     7921 aaaattaccc ctttcgtcta ccacaatcaa gaaggagaag gaccactctt tccctcttct
     7981 taattctatt atatcgaact ctaggttatt tgcaaattaa atcgctatca aatttccaca
     8041 tctaattcat ctcgccaaag aaatggttcg gatgattttc ggctgtgcga cctgcaaaca
     8101 atctgctgcg tggaaaagag tctgtcctgt tgtatagaca tcatcaataa tcaatatttt
     8161 tttattttgt atcttagaaa aggtgactgc aagttcaaaa ggttgaggca tctccaatct
     8221 ttcctttcgc gtgttttttg cttgagccag cagatgcttc tctcttttga gtagcatctg
     8281 aaaaggaatt cttgctgctt tcaacatctc gcttacttga ttgaatcctc tttctgttcg
     8341 ccttttttca gataaaggta ttggacaata gatgtatgtc gggtattctt tttttatctg
     8401 cgtccattta tatgcaaagg tgcctgctag tcggatatct cccataaact tgtattgatt
     8461 gagccaggcc ttcatatttt catcgtacac aaaaaaggca cggtgctgaa aatcataggt
     8521 cggatatttc ttcttccatt cttgacagtc actgcagtaa acgggaatag actttacttt
     8581 ctttttcgta ctctctagat tgaaatggat tggtttttga cagccagaac agcctcttgt
     8641 ttccaataaa tgaaattgtg cgcgacaaac tgaacaagtc tgaggtaatc tgtatttcga
     8701 aacaccaata atctcgctga atgttaaatt ttggaagata tctttactgc agcaactgca
     8761 cctcacagca tcttcttccc ttcttcattc atttctttga tttcctgaca agcttttcga
     8821 atagctgcag ttttttcttc atagaaaaag tatacttgac tgttcgtata aggcccttta
     8881 cgatcggctc ttcctgcgat ttgtaccaaa gaagatttag taaaaaccgg atgatccgct
     8941 cctaaaacga taacggatat tctttcaaat gtgactccac gttcaaggat catcgtggtt
     9001 aacagtatat catattttgc ttctcgcatt ttctggactt tttccgcccg ctgattgtct
     9061 cttgaagaaa catctgtgat ctcatatctc ggaagagcct cttgaagtgt ggctttcaaa
     9121 cgatgcatca tcgtgatact tggacaaaat aaaaggatat ggttttcttt aattaattga
     9181 tgaatgatcc gcaccaaaga ttttatcttc ttcttgttct tgcatttttg tcgccaatta
     9241 ttcatgaaaa ccaagacggg ttcaggaagc aaacgccgat gaaagcgtaa agcaagtttg
     9301 tggatgtcaa acgtctgctt gatttccttt agtaactttt tatcaggtgt ggcacttaag
     9361 tagatgaatc gtccgtctgg tttaatcgct gtttgcacag catgagctaa tcctgcatcc
     9421 ccgttataag gaaacgcatc gatctcatcg atcactatta aatcaaagga ccgataaaaa
     9481 cggtatagct gatgaatcgt acagataaca aaagaagtaa aggtataagc cgttgctgta
     9541 ttcccatgga gcaacgtgat tttttcttct ggaaaaacat aacgatagcg taaaaacaat
     9601 tcattacaaa catcgactcg cggtgtacaa atcgctactc ttcctccagc ttttaaagtt
     9661 tccactaaaa taggataaag catctctgtt ttccctgcac ctgttacagc ccaaataaga
     9721 tgatgtcttt tttctgaatg gcaaagtatt ctctttgaga tttccgtttg tttttcagta
     9781 agctgtcctg tccaaaaaaa gacgacttct cgaggttttg cttcaggctg atcaaacaaa
     9841 tataaatctt gttgagtgtc gcatcgccct aaatatagac aatgtgtaca aaaataaccg
     9901 taatctgcaa gctgtccatc aatgacggac gtcctcattc cacagcgatg acaaagaatc
     9961 cagttgccca aacgttccac tgctgcgatc ttctcataat ttggtaaatt tacctcctca
    10021 cagtctattg cttcaatatc tctcgtcaga agccttctac cccaaaaatg attcatattc
    10081 ttcgccctaa aaaaatatac gaaaaaacaa gcgatctatt ttttggatcg cttgttcgat
    10141 agttatactg ttctatttat tcacctatga gcgtgattgc ttgatctaac actttttcgc
    10201 cattcatcat accataatca gccatattga tgacatctaa aggaatgcct tttggttcca
    10261 gacgtttctc aaaatcagct ttcataaagc gaacttgtgg gcctaataac aacacatcca
    10321 cgtctttatt tgcaaggttg ttatctgcgt cagaagcgga cacagcgaaa atatccgagt
    10381 cgattccttt tgcttcagca gctttttgca tttttgttac tagtaaactt gtgctcatac
    10441 cagccgaaca tacaagcatg attgtttttt tagccatact tttctctcct ttttaaaaca
    10501 aactatgcaa acgttttctt cgcgtctgtt actctattat ataaaaagaa agaaatttgt
    10561 caacgttttc taaacgcggt ttatttaatc aaatagccaa ctagatttca ctaatttatt
    10621 gaacacgaca tattctttgt ccaaattgat gacaactact tcatagcctg catttatcca
    10681 gccataggca cctttttcag ggtgtttata atcatttgcc caccaatctg tactttccct
    10741 tgctgttttg ggaagtccgt tactgggaaa cagcagttcg tcaaattggg taaaggtcaa
    10801 cgttacttga gagccgccat tgttcttcaa ataatgcgta attgcatcgt actttttcat
    10861 tcgttcctgc atccccagat ccacctgcct ttgtctttat tatcgcatac tacagcaatt
    10921 gcgcaaccgt tacagtgttg aaacaaccta gaactttatt tatactgaat aaagaaaatg
    10981 aaaaaaagag gtgacaagat gatcgagcgc tatcgtacaa taaaagaaaa tggacaaagt
    11041 gagattgaga tcaaaaaatc acgctttatc tgttctctta gacgaataga atcagaggac
    11101 caagcaaaaa gtttcattca agaaataaaa aaagaacatt ggaaagcgaa ccataactgc
    11161 agcgccttca tcattggaga aaaaaatgaa atccagcgaa gcagcgatga tggagaacca
    11221 agtggaacgg cgggcgtacc gatgttggaa gtcctcaaaa aaaatcaact gatcaatgta
    11281 gctgcagtag tcactcgata ttttggagga acgaaactcg gcacaggcgg attaatacga
    11341 gcatacactc atgcggtctc tcaagcattg tctgacattg gaatcgtaga aggtaccttg
    11401 caacaggaga tctctgtgat gatcacgtat ccgcaactag gaaaattaca gaattatctt
    11461 gaactccaac atttttttat aaaagaaatc caatatactg atcaagttag cgtcatctgt
    11521 gctgtagatg aaaaaagtgc tgaccacttt aaagaatcca tcattgagct gctgaacgga
    11581 caagtccaat ttgaagaggg ttctgtttct tatcacgagc aactgataac tgatcatata
    11641 taaaaacagg tgattctgat tctcttttat ttctaataag gaatcaggga tcacctgttt
    11701 tttatttttc caaataaaac tcaaaacgat ttcctacgta ttgcgtacgc acatactcaa
    11761 acggcgtacc gtcttcaaaa taagaaatct ggcgcaaacg aagaactgca tctcctcgtt
    11821 ttacctctaa atagtcagca attttctcag aagccaacac agcagtaatc gtttgattcg
    11881 ctggaccgat ctttttcccg cctttttctt ctagcgtgcg atacaacgat tcagtaatct
    11941 ctgctttgct gtagccatcg atcagctttt gtggaacact ggcaacttca aaacaaatag
    12001 ggacatcatc tgcgaaacga atacgttcca ttcgcaagat cgtgtcatct ttactcaagt
    12061 ttagtttttc catttcactt gagcttggcg aagtcaagaa ataagagatt gtccgactag
    12121 acggtactcg attttgtgat tccataattt ctgtaaaact tgttgcacca gacatttttt
    12181 cttgtacttt ttctcgcgca acatacgtac ccgaaccgat ctttctttct aagattcctt
    12241 catctgccaa gttttgaatt gcttgtctca acgtcatacg gctgacattg aatttcactg
    12301 ctaattctct ttctgaagga agacggtctc cgactttcca tataccctgt tcgatttcat
    12361 tttttaattg gtcatgaatc tgtatatata caggtaattg attcgacata acgtacaccc
    12421 gtcctttcgt ttcttctatt ataactggta tatacctgat tcacaactga aaaagataaa
    12481 ggtttctatc catcaacaga ggatttagac acacatcgtt ctccctactc ctattgtcag
    12541 aaacaatttt ttagatatgt tcaacctaga aaataacaaa agaccctaaa atcgatgaaa
    12601 atgccgactt atggatcttt tgttattatt agcggttttt agccatccat ttttttgtat
    12661 gattcgttgg gcgttttgat tttacctgga ccgcttttcc gccttttttc ggaatattca
    12721 cttcaatatt gtatttttct gatttgacca aatcatataa cttttcagct gcttcgtcta
    12781 ctttttcttt ccatttcatg tggatccctc caattcttgt agttaactta actctatctt
    12841 aattcagcag aatcttcaag gaacaaggta gcaaagcaaa aaaaagagct ctcgaactaa
    12901 c
//
LOCUS       DACCCS010000076        12670 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.078, whole genome shotgun sequence.
ACCESSION   DACCCS010000076 DACCCS010000000
VERSION     DACCCS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.078"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            6..305
                     /locus_tag="IVK82_002376"
     CDS             6..305
                     /locus_tag="IVK82_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6393414.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IVK82_002377"
     CDS             378..1739
                     /locus_tag="IVK82_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP6393415.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IVK82_002378"
     CDS             1761..2021
                     /locus_tag="IVK82_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393416.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IVK82_002379"
     CDS             2035..2787
                     /locus_tag="IVK82_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP6393417.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IVK82_002380"
     CDS             complement(2881..3486)
                     /locus_tag="IVK82_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6393418.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IVK82_002381"
     CDS             complement(3539..4513)
                     /locus_tag="IVK82_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP6393419.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IVK82_002382"
     CDS             complement(4891..5670)
                     /locus_tag="IVK82_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6393420.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVK82_002383"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVK82_002383"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP6393421.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IVK82_002384"
     CDS             7161..8558
                     /locus_tag="IVK82_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6393422.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IVK82_002385"
     CDS             8639..9079
                     /locus_tag="IVK82_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6393423.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IVK82_002386"
     CDS             9227..9553
                     /locus_tag="IVK82_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6393424.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IVK82_002387"
     CDS             9554..10303
                     /locus_tag="IVK82_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393425.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IVK82_002388"
     CDS             complement(10383..10838)
                     /locus_tag="IVK82_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP6393426.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IVK82_002389"
     CDS             complement(11265..11540)
                     /locus_tag="IVK82_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393427.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IVK82_002390"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IVK82_002390"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP6393428.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4055 a         2176 c         2586 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttgctct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACCCS010000077        12632 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.079, whole genome shotgun sequence.
ACCESSION   DACCCS010000077 DACCCS010000000
VERSION     DACCCS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.079"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            556..756
                     /locus_tag="IVK82_002391"
     CDS             556..756
                     /locus_tag="IVK82_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6393429.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            1324..1887
                     /locus_tag="IVK82_002392"
     CDS             1324..1887
                     /locus_tag="IVK82_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393430.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1948..2292)
                     /locus_tag="IVK82_002393"
     CDS             complement(1948..2292)
                     /locus_tag="IVK82_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393431.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(2389..2634)
                     /locus_tag="IVK82_002394"
     CDS             complement(2389..2634)
                     /locus_tag="IVK82_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6393432.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(2704..2958)
                     /locus_tag="IVK82_002395"
     CDS             complement(2704..2958)
                     /locus_tag="IVK82_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393433.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(3122..3445)
                     /locus_tag="IVK82_002396"
     CDS             complement(3122..3445)
                     /locus_tag="IVK82_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393434.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            3632..3705
                     /locus_tag="IVK82_002397"
     tRNA            3632..3705
                     /locus_tag="IVK82_002397"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3666..3668,aa:Ile,seq:cat)
     gene            complement(4090..4587)
                     /locus_tag="IVK82_002398"
     CDS             complement(4090..4587)
                     /locus_tag="IVK82_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6393435.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(4627..5436)
                     /locus_tag="IVK82_002399"
     CDS             complement(4627..5436)
                     /locus_tag="IVK82_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6393436.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            5770..6627
                     /locus_tag="IVK82_002400"
     CDS             5770..6627
                     /locus_tag="IVK82_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP6393437.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            6620..7522
                     /locus_tag="IVK82_002401"
     CDS             6620..7522
                     /locus_tag="IVK82_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP6393438.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGFMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            7555..7905
                     /locus_tag="IVK82_002402"
     CDS             7555..7905
                     /locus_tag="IVK82_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP6393439.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7939..8877
                     /locus_tag="IVK82_002403"
     CDS             7939..8877
                     /locus_tag="IVK82_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6393440.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8955..9848
                     /locus_tag="IVK82_002404"
     CDS             8955..9848
                     /locus_tag="IVK82_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6393441.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9886..10449
                     /gene="amaP"
                     /locus_tag="IVK82_002405"
     CDS             9886..10449
                     /gene="amaP"
                     /locus_tag="IVK82_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP6393442.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            10463..10651
                     /locus_tag="IVK82_002406"
     CDS             10463..10651
                     /locus_tag="IVK82_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP6393443.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            10664..11566
                     /locus_tag="IVK82_002407"
     CDS             10664..11566
                     /locus_tag="IVK82_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6393444.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            11598..11840
                     /locus_tag="IVK82_002408"
     CDS             11598..11840
                     /locus_tag="IVK82_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6393445.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(12096..12467)
                     /locus_tag="IVK82_002409"
     CDS             complement(12096..12467)
                     /locus_tag="IVK82_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393446.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4472 a         2118 c         2372 g         3670 t
ORIGIN      
        1 tttccactcc ttatgcagat ttttttaatt cttgcactgc ttgttcgatc gtaacacctg
       61 tcgctacatg attggtattt tctttgtcaa aaacgacaaa tagattcaaa tctgtatcca
      121 aggttactcg atattgtata tctttgttat agaatgtcat agccattctc ctttctttaa
      181 atacttgctg aaaggcaacg aacgcgttga taacgaaatt ccatttataa gtataccacc
      241 aattgaaagg ttttcatagt taagaaattt taaagtcaaa agttgcagat aaaagcaaat
      301 aaacaagaaa acaaaaagtg aatcaatcgc ggttattcaa ctttttcttc gattcgaaga
      361 caatagaaga caatgaattt tatatttcat tgcattatcc ttgtgttatt agaaaaagca
      421 tgatatatta aatgagtaat ctattttgaa acgtaatttg agcgatatat ctacatagat
      481 aaaactcctt ttgcctatca aatatttatt gcaacgaaaa aacgtacatt gatacgtatc
      541 aggaggaatt tatttatgaa taacggtaca gtaaaatggt tcaacgcaga caaaggtttt
      601 ggctttatca ctggtgaaga tggaaatgat gtatttgcac atttttcagc tatccaaggt
      661 gacggcttca aaactttaga agaaggccaa gcagtgtctt acgatgttga agatggtcaa
      721 cgtggccctc aagcaacaaa cattgttaaa gcttaataaa aattgacttt taaccgtctt
      781 ttcgaaagaa aagacggttt ttttgtacga aaaaattcat tggtcgttga atgggctgaa
      841 tagtagtgta taaaacaatt ttgtggagcg tgaagctttt atggtagagg aaaatacgaa
      901 taaagtgatg ttgataaaaa gattcctatc ttgaatatga atcgagatgt cttgaaaatg
      961 aatgagtttg accaatcatt atcctatcga aatttacagt ttcgttcgat tggtctgtca
     1021 attaatgaaa aagtaaaaaa gaatatgttc taggaagagg gcggcacatc taaattttta
     1081 agtaacggac agttcaaata tcaagcattg aaagaggcta tcctactgtt tgaaggaata
     1141 cgaatatata aaaacgacaa agaggaaaaa gtagttcatt aaatcccttc aagtgatgaa
     1201 agaatgagat ctaataaaac ctgatctttc atctgttttt ttaataacgt aataattttt
     1261 ttattttttg tgctttcgcc gttattgtag agtgatttag cattcttatt tttaaaaata
     1321 actgtgttat atattaaaaa aggcataata gttataagct tttttaaaga atatttagaa
     1381 attattaatt ttcgtctgaa aacaaaaaaa gaaagcgtta ttattttagt ctattttcag
     1441 ggggggaacc ttatgataaa aaaactattt ttactatcaa tattaacgct agcaatggca
     1501 ggttgttctg actctggctt agcaactgaa ctgatcacaa aatcaactga agccacgaca
     1561 gaggagttaa aatgtggaaa cgatcacagt gactacagag gcttaaatcg acgatgcatg
     1621 ggaatattag gcagaagcaa ttgtaagcat cgatgtagta atcgaagaag gtgtagaaga
     1681 agcaacagaa aaatcaaaag aaagatcaaa agaagcaatc tgtgctgtgc atcgtataca
     1741 gacgtcgaag atgcggtact tttaaaccac gtcaggagat gttcatttat gtctagggaa
     1801 gttggcgatg aaccaaaact agagtacgct tttagttttg cacgtgagga tgaagataac
     1861 cctaaaactt acgacatttt agcttgatct aagatggtta aagcttttta atgaagtaag
     1921 aggagataga aggcttttct agttatatta ggtaagataa attccttttt gctccaaatt
     1981 tgctaaacat tctgctagat aaacttcatc gtgttccgga taaatcggca cgttgcgctc
     2041 tacagtaggt aaatcaaaaa atgcttcgca tgatttgcca cgatacatag gatttttcca
     2101 ctctggtgct gggctgtaac cacgtttttc catttcatcc atgacaagct gatgatattg
     2161 gaataatcta taaggagaat agtgaaaaac ataatccaca gtagcatgtt tctttcccca
     2221 gccatttcct cttaatgcac agcattcccg atgttgtcct aaaagctgtt gacgaggaag
     2281 ctttgaaatc aatttttcat gccataatca catagaaatg tctcctttat atatagattg
     2341 ttaaccttag acgttaaatg aaaaaaccag caccaaggct ggaaaaaatc acttcagttt
     2401 gcttgctgtt tctgagcgac caattaggta atcaacggaa atatcgtaaa agtcagcgat
     2461 atagattaat tcaatgatgc ttggaatttc ttctccttct tcgtacttac gatatttgaa
     2521 taaaggaata cttaacgcgc tagaaacagc tacttgagat aaagatcggc cagttcttaa
     2581 gaaattcaga cgacaacgta aagtatccag taaaatagct tcttcgatac tcatttaatc
     2641 acctttcatt atagtagaat agttataatg actatagaaa gctacacatg caatcaatag
     2701 tactcaatgt tttttattta aacgtatcaa ataaagaatg acgatcaata aagacaaaac
     2761 cagtcctgat aattcttcta taaaaacact atcgggttca aagtagttgt aattaaaaac
     2821 tttccacata aagtagatag ccggaagagt caaaatagct aaataaaacc gttctttgtt
     2881 ccaattcata agataaacaa tacttgcgac cagtgcgacc gcaggcaccc aaaacaaaac
     2941 tctcaccaac gtaatcatgt aacggactcc tcaacgattc atataataaa agataacatg
     3001 ttattgcata catcacaaat gaaatgctag gaaaataaaa gaagaataga tcaatgaaaa
     3061 atgaaaaagc agaacagttt tctgctgata agcaggaact gttccgctga atgtgtctta
     3121 tctatcgaga aaaaatcaat ggattgatat ctaagtaatt caatacatat aaaaagagga
     3181 cgatcgcatt cactaaaaag gaaagaatga tgatcccata gcgaatggaa acatatttaa
     3241 caaatacttt tgtttgaata cgggcatatc tttttccttt atagctgagc atcatgacaa
     3301 gaagcaaaag ggtaatcaca ccttgaaaaa tcattccaat aaacagaatt cctgaggttt
     3361 gataatttaa ttggagaaaa taagctgctg ccgcaactaa atcgaataaa atcgcaataa
     3421 atggcaaaat aatcactcct aacattgaga ataaataaaa gaataacatt ttttctaaaa
     3481 taagcaaata aaaattaaag taacaaaata gttatgacac aagtaaaaga aataagaata
     3541 acaatattat tttacttata ttttgaaact tgtgagagtt tgtcattcgt gctatagttg
     3601 aaaagtaaca aaaataaatt ttatttatga tgtaccttta gctcagttgg ttagagcaga
     3661 cggctcataa ccgtccggtc gtaggttcga gtcctacaag gtacataaga ggttgtgaaa
     3721 aagctgccct gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttac
     3781 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
     3841 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
     3901 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
     3961 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
     4021 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
     4081 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
     4141 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
     4201 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
     4261 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
     4321 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
     4381 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
     4441 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
     4501 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
     4561 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
     4621 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
     4681 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
     4741 gctgtaattc aacatatcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     4801 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     4861 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     4921 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     4981 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     5041 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     5101 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     5161 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     5221 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     5281 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     5341 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     5401 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     5461 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     5521 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     5581 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     5641 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     5701 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     5761 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     5821 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     5881 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     5941 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     6001 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     6061 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     6121 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     6181 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     6241 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     6301 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     6361 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     6421 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     6481 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     6541 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     6601 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     6661 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     6721 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     6781 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     6841 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     6901 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     6961 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     7021 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     7081 atcaatgcgt tatttcaact caggttttat gctgatcaat gtgaaaaaat ggttagatga
     7141 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     7201 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     7261 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     7321 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     7381 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     7441 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     7501 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     7561 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     7621 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     7681 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     7741 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     7801 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     7861 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     7921 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     7981 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     8041 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     8101 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     8161 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     8221 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     8281 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     8341 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     8401 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     8461 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     8521 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     8581 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     8641 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     8701 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     8761 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     8821 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     8881 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     8941 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     9001 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     9061 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     9121 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     9181 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     9241 acgtgaaagt tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     9301 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     9361 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     9421 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     9481 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     9541 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     9601 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     9661 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     9721 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     9781 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     9841 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     9901 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     9961 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
    10021 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
    10081 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
    10141 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
    10201 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
    10261 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
    10321 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
    10381 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
    10441 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
    10501 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
    10561 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
    10621 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
    10681 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
    10741 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
    10801 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
    10861 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
    10921 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
    10981 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
    11041 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
    11101 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
    11161 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
    11221 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
    11281 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
    11341 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
    11401 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
    11461 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
    11521 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
    11581 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
    11641 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
    11701 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
    11761 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
    11821 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca
    11881 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggttgggcat
    11941 attatcgaag atgcaagctt ttgaacactg tttatacgga ataagaggct gtgacaagac
    12001 ttttgtcaca gcctcttatt tatgcgggca aattcccaat tagattttta aaagtttttc
    12061 cttaaatgtg tctttattga tacagagaag cttctttaat aaaacaatgc ctccctaaat
    12121 tttcttgaga taggaaaggt gaacatacaa aataatacga ccactttgaa aacccagatg
    12181 atccacaaat ggatcgtatg atactgatga agtaaaaagc aatggatcag aaactcaaag
    12241 agattgaaaa agacaaggaa gaaataatat ctctttttta ctcttgtctg ttttagccaa
    12301 taatagctcg taaagtaatc tagacaaaca aatagaagta aacaaagttt cgtaagagca
    12361 gcaatcgtat catgttgctc tgctagacga ttgacgagca ggaaaaatgt tggtaatgga
    12421 atatagatca cgagtaatga aagaaaaaag taaacaataa atggcatact attcctccta
    12481 attgtgtgag ttcgccctca ttttaaaata acgatatttg tttgtgaagc tttttcgtgt
    12541 cgtaacaaat gttatctaat gtatttttaa ataaataatc attattatca aaataatgtc
    12601 agactgtaga caaagtccat tttatggatg at
//
LOCUS       DACCCS010000078        11518 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.081, whole genome shotgun sequence.
ACCESSION   DACCCS010000078 DACCCS010000000
VERSION     DACCCS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.081"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(19..219)
                     /locus_tag="IVK82_002410"
     CDS             complement(19..219)
                     /locus_tag="IVK82_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6393447.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(671..991)
                     /locus_tag="IVK82_002411"
     CDS             complement(671..991)
                     /locus_tag="IVK82_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393448.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(1292..2410)
                     /locus_tag="IVK82_002412"
     CDS             complement(1292..2410)
                     /locus_tag="IVK82_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393449.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            2881..4188
                     /locus_tag="IVK82_002413"
     CDS             2881..4188
                     /locus_tag="IVK82_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP6393450.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFDNLF"
     gene            4307..5506
                     /locus_tag="IVK82_002414"
     CDS             4307..5506
                     /locus_tag="IVK82_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP6393451.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(5533..6801)
                     /locus_tag="IVK82_002415"
     CDS             complement(5533..6801)
                     /locus_tag="IVK82_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP6393452.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLITLTL
                     FGTITTHLFGGSVGREGTAVQMGGSLADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6964..7611)
                     /locus_tag="IVK82_002416"
     CDS             complement(6964..7611)
                     /locus_tag="IVK82_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP6393453.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILNHELPEIDTFLTTVLDVKLTKAQAERALEKLKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(8019..8522)
                     /locus_tag="IVK82_002417"
     CDS             complement(8019..8522)
                     /locus_tag="IVK82_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6393454.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(8645..9409)
                     /locus_tag="IVK82_002418"
     CDS             complement(8645..9409)
                     /locus_tag="IVK82_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP6393455.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSADQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            9516..9791
                     /locus_tag="IVK82_002419"
     CDS             9516..9791
                     /locus_tag="IVK82_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP6393456.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            10507..11457
                     /gene="yidC"
                     /locus_tag="IVK82_002420"
     CDS             10507..11457
                     /gene="yidC"
                     /locus_tag="IVK82_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP6393457.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         3490 a         2365 c         2075 g         3588 t
ORIGIN      
        1 ttttttagta taggttaatt aagctttagt aacgtttgtt gcttgaggtc cgcgttggcc
       61 ttcttcaaca tcgaaagtta ctgcttggcc ttcttctaaa gttttgaatc cgtcgccttg
      121 gatagctgag aaatgaacga atacatcgtt tccgttttct gcagtgatga atccaaatcc
      181 tttttctgag ttaaaccatt ttactgtacc tgtttccata ataaaaatcc tccttgtgta
      241 tcaacacata ttttgcaatt acttgaatgg tgtatatctg gaagatgttt atttgaaaca
      301 agctaccaca gattaccgaa caaaaactac tcctatagta tatcataccg tttacaaaaa
      361 agctagagtt tttcgacttt ttttattaaa aaaagttttc tcagcgatta cataaagtga
      421 aaaaacgaac tttttttaaa ataaacgaca tattgttcag ttggtgtttt tttgctctga
      481 tgttcttctt taaactaaaa aaattattct ttctgactac ttatcggttc tgtttcaacg
      541 gtcgaggctg gggaagattc tttagtaggt ctgatgttct catggtattt ctgatagcct
      601 atgacagtga ccagaagaac aaggcaaacc aatacgatca acgagattag tttttctttt
      661 ttgctcatat ctatacctca taaataacag gatgatgtcc atcatatttc tgagtatgag
      721 taaaagcaaa agctgcagct gtagaatgta agaaatgcca gcaactttca taatcacagg
      781 tcggacagta ccaaaagtaa ccaaatcttg gctgcaaaga ccgttcttct gtttcaggaa
      841 tcgcagaatc aagatctgtt gtctgtacat aaacattttt gtacagacgt gtgcctgtaa
      901 taggagaccg ctgtgtttct tcagcaaaaa ctggctgact tgttaaactt agggatagaa
      961 acagcaaaca ccataacagt ctttttttca taaagacctc cttatcgata agtttttccc
     1021 aattaaaatc atatctcaag aacgcccgtt attcacattt tgttcataaa tgatgttttt
     1081 tgtatcataa tcgtcaaaaa gaggtcgtga ccacgtcaca acctcttttt taaacggtgt
     1141 tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag aaataatttt
     1201 tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct tccttaatac
     1261 tcaggtcaaa acactgattt cacaacctct tttaataacg aagaatcgct gtcagactct
     1321 tttcatctgg cgcatctttt tgatccaaaa taaacacttt cccgccatta atgatcacat
     1381 catctgctaa tgcattcaac acttgacgcc gatcatattc tgtttcagga tcttcaccga
     1441 aaccatcagc taagtttgac gtagcgataa ataaatgtga cacttttcct tctttcgcag
     1501 ctggaacgat atcaactaac tgatcgatga atttcttatc taagagttta ttgtattctt
     1561 ttgattcttg agctgcgagt tcacttgaca gcttcttcgc cccttcttcg atatctctaa
     1621 aggatagctg cgcaggagaa gcagaaatcg caacgtctgc acaataatag ggatttttag
     1681 caattttttt gaacatcgtc tgattttctg gtaatgcaaa taaataaatc ggccattgtt
     1741 caggattttc gaaagaatcc tttaagtacg tatcaacagc ttggtaatag ttgacccaat
     1801 cgatctctac ttcttcatct tttggattta cgccatggaa agttactcca tctcgggctc
     1861 cgcctcctcc attagaagag aagttcaaac cgccacctgt cagttcagat cccaatgcag
     1921 taacgacatc aaccggtgcc ccttctggta gagatacttc ttttacttgt ttgttgctga
     1981 cctcatacaa tttcatggag tcgcggttca atcccaataa ataataagaa tagttgaact
     2041 gcccattttt gataatagcc agtaaataag gaagatcact tacatagtat tgatcgtcta
     2101 ccgggatact caaacgatgg atataggtct ggtcagcagt tataatgacc gctacactcg
     2161 tagttgcact acgccaaaac gaaggatctc ctaacaaatt ttcaattttt tcttggaacg
     2221 gagaccagtc atgttcggaa tattttttct caaaacgttt ctttgcttct ttcgcaaaat
     2281 ttttgaattt gatctgatct ttttccacat cttgatgggc gacatgcgta ttcagcatga
     2341 tcgtgacgaa tggtccttta gcgccttctg aagtcaattg tgtcaataat tctttatcct
     2401 tttttgccat aaacgtaccc ctcctaaaaa aatcattcct ttgtttctat ctaaagtcta
     2461 ccacaacact atttttccat acaataaaac gtatctaaaa aaataaaacg gtttctacgg
     2521 ttttaaattt agctattttt ttaagttatc ttcataattt cctcaattat ttagccccag
     2581 tcttcaacta ttcttcatga aatgctgata aactaagaaa aagaataaaa gaggtggatc
     2641 ttatgcattt aattatgatt ttcagtttat taagtattat cagtttattt gctttcagcg
     2701 cttcccgctt attagatgcg gaaacacggc ctattcctgt aaaagtacgt aaaaacgacc
     2761 gctaaacttc cataacgcgc gttcttatcc taaagttttt tcagaaacat tgaaactctc
     2821 tcccctctct gataaaattt taatttgtag cgaaacaaat taattacaag gagatcggat
     2881 atgtccataa aaaaaacacg tctagtccta ttattggcta gtctcattct ttgccttgct
     2941 tcttttctac cagctgttgc tgtgcaagct gaagatacgt tcaaagtaaa tgcaaaagca
     3001 gcttttgctg tagatgcgca aagcggtaag attctttatg atcaagatgg agaaaaaacc
     3061 atggggatcg cttcaatcac aaaaatcatc ggtctttaca tcgtccttga tcaagtaaaa
     3121 gaaggaaaac tgtcatggga tgataaagtt tcgatttcag attatgcaga aaatttgagt
     3181 atcaccccag atttatcgaa tgttcctttg cataaagaaa acacctacac tgtcaaagaa
     3241 ttattcgact ctgcaatcat ccagtcagcc aatgcttcaa tggtggcctt ggcagaaaaa
     3301 atctctggca gcgaagctaa attcactgaa cgaatgaaag aacaattgaa agattgggga
     3361 atcaaagatg caacaatcgt taatgcatca ggattgaaca actcttatct tggcgaaaac
     3421 cgaccagaag gcactggaga aaacgacgaa aatcaaatgt ctgctcaaga tgtggccatc
     3481 gttgcccgcc atttgatctt ggatttccca gaaatactgg atgtatcaag tactacgacc
     3541 caaatgtttg gcgaaaatac gcaatcacca gtggaaatgg tcaactggaa ctggatgctc
     3601 ccaggattcg ttaattacaa ggaaggcgtg gacggattaa aaactggtac gactgagctt
     3661 gcaggtgctt gtttcgttgg tacgatcact aaaaacgatc aacggatcat cactgtcgta
     3721 ttgaatgcgg acggccatgc ggaaaacccg agtgcccgct tcaatgaaac agaccgtttg
     3781 atggactact gctatgacaa ttggtcagaa aaagagctcg gaaaagctaa cgcaagtatt
     3841 cctagtctaa aaaccattga tgtaaaagac ggaaaagaat caacagttcc tgttgtgctt
     3901 aaaagccctg taaaagtatg ggtacgaaac gatatggata ctggcaaatt aacgatcacg
     3961 ccaacacttg acaagaagca agtcactgac aatgagatcc aagcacctat ggataaaggt
     4021 acaaaaatcg gtaccgctac gatcaccttg gcagacgata aattaggcta tttagaagac
     4081 aacaatgcac catcaacaga gatcattacg gataaaacag tggaaaaagc gaatatattc
     4141 gtgattggct ggcgacgcgt cgcagaattt ttcgacaact tgttttaatt gaacaataag
     4201 aaaaacatct ttccatcgga aagatgtttt tttgcttttt ctagtttaaa taacagcttt
     4261 tttcatctat tttcgttata ctgtataaga aaaaggagtt gaatttatga atctaaccag
     4321 agatctgatc tttttagttc tctatcttat tggggtattc tctatcgttt attggcgaaa
     4381 acagaagaag gatccttcct tgttctttta cggcagtacg tggacattta cggtaatctt
     4441 aacttatttt gtcgtgttgg aaatttttta ttcgaccttt tattttttga ttccattgtt
     4501 gttctttagt atctttgctt tttcttattt cacagaaaaa cgaaggctgc tgaatggtct
     4561 tttattcaat gttttcctca tcagtttcgg catctacttg tttgtcttgc tgtatgaaac
     4621 gcaaaatatt ttccttggcg gactgatcgc cttgatcacg attccattgc tacttgtcct
     4681 tttatttggt atttatggat tgattgtttt tcttttttgg aacggggtca cagttttacg
     4741 tagagaatcg cattcattag ctaatctttt aacgttgata ctagccattt ttcttacact
     4801 gtttcttgtt tttgactttt tcttgttaaa atatttgccg caatggataa atgctttgtt
     4861 tttctgcgtt cccctgatct tgatctatct atttatcgta ttctacaact ttttgactgt
     4921 ctcattcttg tatcagttca accggcctcg atacaatcaa gacttcatcg ttgtgttagg
     4981 cgctgggttg atcaatggag aaacagtttc tcctcttcta gccaagcgaa tcaataaggc
     5041 aattgctttt tatcgggctc aaagccgtgc tacattaaat ccgcccatct tattgatgtc
     5101 tggcgggcaa ggagcagacg aaaaagtacc agaggccatt gcgatgaaac aatatgccat
     5161 ggaacaagga atccctgaaa gagacattct tgtcgaaacc aattcgacaa ccactttgga
     5221 gaacatgctc tattcaaagg aaatcatgga tcagcaaatg aaagggccat accgcgcgat
     5281 ctttagttcc aacaattacc acatttttag agccggtctt tatgcgtggc aagccaaatt
     5341 gaaagcaaat ggtattggtg caaaaacagc tttctactac ttgcctaatg cgtttttaag
     5401 agaatacatc gccatcttga tgatgaacaa aaaaagacat atgatcattt gcggattgct
     5461 tttcgcaggc tccgcattct tatcgctgat tactttactt ttctaaaaaa acaaggtgtg
     5521 acaaaacata tgtcacacct tgtttttttc tttcattctc ttaactattg gcaaaaagag
     5581 tatccctttt ttgaccgcta cttgctgggc agaataaata ccgctgttgc ctgaacacat
     5641 aaagctgatc aagcagatca taaagaagta tactgccgct tgtgaaccga acaattcgat
     5701 ccccatcaca aagcaggcaa tcggtgtatt tgtcgcacca gaaaatactc cgataaagcc
     5761 caatcctgca agaaaaggaa cagaaacatg aagcaaaggc gctagtgctg caccaagcgt
     5821 tgcaccaatt tcaaacaatg gtgtcacttc tcccccttga tatcccgcgc ctaaagacaa
     5881 aactgtgaag aataacttcc caataaaatc atagacatgt gcctgtccat taaatgattc
     5941 ttgaagtaac ggaagactca atccgagata acgttgtgtt tgcaaaatca atgctgctgc
     6001 cacaactaca gctccaccta tcgtatttcg ccaaacagga tgaggaatcc attgtgcata
     6061 tcgcgctttt aaaaagacga tcgaacggct gaatacccaa ccaatcaaac caaaagcaat
     6121 cccagcaaca aaaactttga agaacagcaa catactccat tcaggaatca cacccatttg
     6181 gtaatgtaga tgagaaacac catagctttc tgtcatgaaa tttgcaaaca gtgcagcaaa
     6241 gaagctaggg aagatcgctt ctgttcggac tttcccgatt gccaacactt ctaatccaaa
     6301 taatgttcca gctaaaggtg tgccaaatac agaactaaaa cccgcactga ttccactgat
     6361 aatcaagatt tcccgttctg ctttatccaa tcggaacaaa cgtgctgtat tatctgctaa
     6421 cgatcccccc atttggactg ctgttccttc acggccaaca gaccccccaa ataaatgggt
     6481 agttatggtt ccaaacagtg taagtgtaat aagccgcaac gggatttttt cttctccacc
     6541 atttccttga tcgatcacta agttgtttcc acgactagaa agaccgccgt gatacgcata
     6601 gagataagca aaaagcgcac cactaactgg tagtagataa aggagccaag gatgtgatag
     6661 tcggatatct gtcacaactg tcaggctatt taagaaaaaa gcagacaagc tccccatgaa
     6721 tataccgaga atcgctgtga tcaccagcca cttcagcatg tacactaaaa tgcgcattgg
     6781 actcggtaat tgtttttcca taaaaaattc ctcctcaata aaaaaacgcc tactaaagaa
     6841 aggggctgtc cccagacttt agtaggcgtc attagctatc tttcgttagc atttaaaggc
     6901 gaacaccatc acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata
     6961 aaattatcgt tctttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa
     7021 ctgttgatta cattggtcac tatcatgaca aatataattc ccctttttcg tatacgtgcc
     7081 atcagaacct gctttcgttg tagaaagaaa aagcgacaca ttcgatgttt tctgacagat
     7141 tgcacagatt cccttattta tcgttggtga taaagtaccg ctaatccctt ttaatttttc
     7201 atcttcgtaa tagaccatga attttttctg cgtacctgga tcattccagc cgatgtacgt
     7261 atgttcttgc aagtcgattt cattccaagc tggttgtttg agttttttta cttttcggaa
     7321 taatttctcg atttgtggtt tgctaggctg cataaacggc tgaacgattt gcttcagctt
     7381 ctccaatgct ctttctgctt gcgcttttgt cagttttacg tctaaaactg tcgttaaaaa
     7441 tgtatcgatt tctggcagtt cgtgatttaa tatgtctcga atcttttcag ctgctagtgc
     7501 ctgaactgtt tgtaccgttt ttggatcatt gacagatgag tacgcattta ataactgttc
     7561 tacttctctt tttataaaaa aatattggta tggttttatt gtttgttcca tgattttaaa
     7621 gtcctttctt ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg
     7681 tgttacctga agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc
     7741 cctttaatgc ttattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc
     7801 ttgaacagat agtcaactgc aaacagaaaa aaggactgtg acaaaagtcg tgtcaaagtc
     7861 ccttgtttcg aatgaatggt gttcaaaagc ttgcatctgc ggtaataagc cgaaacttca
     7921 caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gaccacttct
     7981 gtcacaacct caatatggtc tattattggt tttacctgtt aaatcgagga ttctttccca
     8041 aagtaacgtt gccatctctt acgcatactt gcagacatgg gctgagccgc ttctttgact
     8101 gcgcagtatt tgtccacgaa tgtcacaata tatccttctt tatacttagg cggtgcgatc
     8161 gttgcacccc acatgtgctt caaaatgata tctcgctcaa gatccgacaa atcggtgatt
     8221 ttttctgcat tctttacagc aattcttgga tggatatacg catgtgtccc ctcgtcaaat
     8281 ttcgtcgtac gccaatcata gtagaacaga tcatgcaaaa gtccagcacg cgctgttgct
     8341 ttagcattgc caccccattt ttttgcgagc agataactat agtaagaaac actgatggaa
     8401 tgctccaaac gagtggaatg aacatgctgt gtgtaattcg ctaatttttt cactgcttct
     8461 gtttctaaaa gatcttgtac ataagacata tattcttcgt cttcacgcca tttttctgtc
     8521 ataattatcg atctgccacc tttttgaatt tcattttctt ctgaaattga tctaagtata
     8581 ccaccagtgt tcggattatt ctagtcgctc tagataaaat agctaaagct taatagttcc
     8641 ttaatcataa agaaaaatga taaagaacga tccctgctgc gactccgaca ttcaatgatt
     8701 ccgcatttcc tttcattggg atatatacgt tctgatcagc cgaagacaag atttcctgac
     8761 ttactccctg accttcgttt cccatgatta tagcgaaatc ctctgttttt tctactttgt
     8821 ctaatgcatg tgcttcttca tttaattctg taccgtacac cgtaaaccct ttgcctttca
     8881 ttacagggat cacttctgcc aaatcttttt gtaacacagg caaatgataa ttgcttccct
     8941 gcatgcttcg cagcaccttt gtactataaa tatctgccgt tcctttacct aataaaacac
     9001 cagccatacc aaacgcatct gccgtacgga tcatcgttcc cacatttcca ggatcctgta
     9061 cattatctag taaaagccag cctcctgaaa aatctggcgt atcttcagat gttaacgtca
     9121 tcactgctaa gatcccttgc ggtgtaggta gatctgacat agcggtcatg acctcatctg
     9181 aaactagaaa agtagaaatc tctttgttta cagccagcca gtctccccat tctttcatac
     9241 ctctctgtgt cacaaaaagt tcttttattt ctgcatcaga cgacgcagct tcttcgatca
     9301 gatggaaccc ttccaaaaga tattctgcct tttcttctct gtatttcttt ttatgtagtt
     9361 tttttaattc tttgattctt ggatttttta ctgattggat ctctttcaca ttcctcacct
     9421 caactgatta tatcatggaa taggtgcttg agcttgtttc atcctaaaaa aatcggtatc
     9481 attgatttat aaaacaggaa agaaaggtga cgaacatgcg caaagttaaa atgaatgtcc
     9541 aaggacgtgt ccagggcgtg ggatttcgct atatgaccaa gatggttgct gatcagttag
     9601 gcgtgaccgg aacggtaaaa aacgaagacg acggctcagt cacaatcgaa gcaatcggaa
     9661 atgacgacat catccaaaaa tttatcgaag aagtcaagaa gtctccgagc ccaagtggac
     9721 gagtgcagta tgtggatgtc caagaagatc cgatgattga ggaacgaaag aaatttgatg
     9781 tggtgggata aggttgtttt tgaagcgctt tgcctcgagt attaaggaga aacattttcc
     9841 aaatagcttc tcatattttg aaatgtaatg acttaatacc gagaggcagc tgataaaaca
     9901 ccgtcaataa ggttgtgaaa aagctggtct gcatcaagca ttaaggactg gttgtgaaaa
     9961 atagctctcc atatttttca acaggctgga cttaatgccg tagatgcaag cttttgaaca
    10021 ccgtcgataa ggttgcgaaa aagctgatct gcatcaagca ttaaggacta gttgtgaaaa
    10081 agtcgcttag ttttgagtat taaggaaaat tttgtgaaaa tggctttcca catttttgca
    10141 taaatttgac ttaataccaa gaaactgact tttgaacatc gtcgataagg ttgtttttga
    10201 ggcactttgc ctcaagtatt aaagacaggt agtaaaaaag ctgatctata acttgcaata
    10261 aatttgctta acccatattt tccagacaga acaaaaaagt ttttagtttt ttaaataaga
    10321 aagaaacact tggaatcact cttttctttc taagtatttc tttcttgatt ttttgtcttt
    10381 tttcaaaatt tttcgctacg aaaaagaacg aatatttata tgaaatttct atgaagttga
    10441 ttgaattcca tgcgcttttt tagtatagtt aagtttgtgt aaaaattata gaagaggaag
    10501 tattctatga ataaatggaa aaactggtta ttgggatccg gacttgtcag tatcctgctc
    10561 tttttatctg gctgtgtgcg gatggattca aacggaaacc ctgacacttc aggaatcgtt
    10621 tatcgagtac tcgtccatcc tatgggccaa gcgattacat atctagttga gaatttcaat
    10681 tggtcttacg gctgggcagt tattgcaatg actgtgatcg ttcgtatcat catcttgcca
    10741 ctaggtatca gtcaatcgaa aaaaacaatg atccaatctg aaaaaatgca agcgttaaaa
    10801 ccacaagttg aagcagcaca acaaaagcta aaagctgcaa caacacgtga agaacaaatg
    10861 gcagcacaag cagaaatgca gcaagtatat cgcgaaaatg gattgagtat gaccggcgga
    10921 atcggctgtc tgcctctatt gatccaaatg ccgatcttct ctgccttgta ttttacggct
    10981 cgctacacag aaggtatccg tgaatcatct ttttacggca tcgatctagg ctcaccaagt
    11041 atggtgcttg tggccatcgc aggggtcgct tatctgcttc aaggatatat ttcaacaatc
    11101 ggtattcctg aagaacagaa aaagacgatg cgtacgatgc tgatcgtctc tcctgctatg
    11161 atcgtcttca tgtcgatcag tgcgccagcc ggtgttacac tttactgggt agtcggtggt
    11221 gtgttcagct gtctgcaaac attcatcaca aacgttataa tgaaaccgcg tttgaaagca
    11281 caagtggcag aagagttgaa acaaaacccg ccaaaacaag tcgttactcc aagaaaagac
    11341 gtgacagaat cagcaaagcc agctgacaac aaacaagcaa caaaacaaat caaacaacca
    11401 aaaaacagta caaacaacaa aggccgcaac gcaggaaagc agcagaagcg gaaataaaaa
    11461 tatatgacaa aaaactgtag acaaataaaa aaatttcttt gttctacagt ttttttgt
//
LOCUS       DACCCS010000079        11286 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.083, whole genome shotgun sequence.
ACCESSION   DACCCS010000079 DACCCS010000000
VERSION     DACCCS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.083"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..882
                     /locus_tag="IVK82_002421"
     CDS             <1..882
                     /locus_tag="IVK82_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6393458.1"
                     /translation="LTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            885..3068
                     /locus_tag="IVK82_002422"
     CDS             885..3068
                     /locus_tag="IVK82_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP6393459.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3065..4066
                     /locus_tag="IVK82_002423"
     CDS             3065..4066
                     /locus_tag="IVK82_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP6393460.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4081..4995
                     /locus_tag="IVK82_002424"
     CDS             4081..4995
                     /locus_tag="IVK82_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6393461.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5240..5356
                     /locus_tag="IVK82_002425"
     CDS             5240..5356
                     /locus_tag="IVK82_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP6393462.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5372..7291
                     /gene="tet(M)"
                     /locus_tag="IVK82_002426"
     CDS             5372..7291
                     /gene="tet(M)"
                     /locus_tag="IVK82_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP6393463.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7389..8762
                     /gene="tet(L)"
                     /locus_tag="IVK82_002427"
     CDS             7389..8762
                     /gene="tet(L)"
                     /locus_tag="IVK82_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP6393464.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9326..10588
                     /locus_tag="IVK82_002428"
     CDS             9326..10588
                     /locus_tag="IVK82_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAP6393465.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10833..>11286
                     /locus_tag="IVK82_002429"
     CDS             10833..>11286
                     /locus_tag="IVK82_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP6393466.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKI"
BASE COUNT         3715 a         1886 c         2365 g         3320 t
ORIGIN      
        1 ctgacttctg atgagaaaaa caaaggctta cttgaccctt atgtaattat gaaaaatacc
       61 aaagattctg aatcactggc tattgatatt ttgacattcc ttacggggat ttcctctcgt
      121 gatggggaac gcttcccaat ccttagaaaa gccattcgtg cagtaaccaa tagtgaagtg
      181 cgagggttga tgaaagtgat tgaagaatta cgggttgaga atacgccact aagtaccagc
      241 atagccgacc atatcgaaag ttttacagac tatgactttg cccatctgct ttttagtgat
      301 ggttatgtag agcagtctat cagccttgaa aaacaactga acattataca ggttgccgac
      361 ttggtacttc ctgacaagga aacttccttt gaggaatata ccacaatgga gttactatcg
      421 gtagcaatgc tgattgtcat tagtaccttt gcgttggact ttatccatac agaccgaagt
      481 attttcaaga ttgtagactt agacgaagca tggagcttct tacaggtggc acaagggaaa
      541 acactatcta tgaagctggt tcgtgctggt cgtgctatga acgctggggt atatttcgtg
      601 acccaaaata cagatgacct cttagatgaa aaactgaaaa ataacctcgg cttaaaattt
      661 gcctttcgtt ccactgacct taacgagatt aaaaaaaccc ttgccttttt tggtgtagac
      721 ccagaggacg aaaacaatca gaagcgattg cgtgatttgg aaaacgggca atgccttatc
      781 agtgatttat atggtcgtgt cggtgtgata cagttccacc ctgtatttga agaactgctc
      841 catgcctttg ataccagacc acctgtgcga aaagaggtgt aaatgtgaaa ccatcaatat
      901 taaacagaat aaaatcaaac tggacgctga aacgtctagg taaagtggca atgacagtgg
      961 ctttcacact tgtgattgcc atttttcttt tagccatgct gggaacggtg gttcaagctg
     1021 cgggcttggt agatgatacg gtcaatgtgg caaatgaata cagccgatac ccacttgaaa
     1081 actatcaact ggatttttat gtggataata gctggggctg gctaccgtgg aactggtcgg
     1141 acggaattgg aaaacaagtc atgtatggac tatatgccat taccaatttt atttggacaa
     1201 tcagtcttta tgtttccaat gcgacgggtt acttagtaca ggaagcctat tccttagact
     1261 tcatttccgc tacagcagat tccattggca agaatatgca gacccttgca ggggttacgc
     1321 ctagtggact atcaacagag ggtttctatg ttggattcct tttactcttg attttggttc
     1381 ttggggttta tgttgcctat acaggactga taaagagaga aaccacaaag gcaattcatg
     1441 ccctcatgaa ttttgtgctg gtttttatcc tatcggcttc ctttattgcc tacgctcccg
     1501 actacattaa aaaaatcaat gacttttcat cagacatcag caacgctagt ttgtcactgg
     1561 gtacaaaaat tgtcatgccc cattcagata gtcaaggcaa ggacagtgtg gacttgatac
     1621 gtgacagcct attttccata caggttcagc aaccgtggct actgcttcaa tataacagtt
     1681 cagacattga aagtattggt attgaccgtg tggaaagcct gctctcaacc agtccagatt
     1741 ccaacaatgg ggaagacaga gaaaaaattg ttgcggaaga aattgaagat agaagcaata
     1801 ccaatatgac gattacaaag acgattaacc gtttaggtac agtcttcttc ctatttgtct
     1861 tcaatattgg gatttccata tttgtattcc tattaacagg aatcatgatt ttctcgcagg
     1921 tactttttat catctatgct atgtttctgc ctgtgagctt tattttaagc atgattccat
     1981 catttgatgg tatgtcaaaa cgagccataa caaagctctt taataccatt ttgacacgag
     2041 ctggaatcac attgattatt acgacagcat ttagtatttc aaccatgctc tataccttat
     2101 cggctggtta tccgttcttt ttgattgctt ttctacagat tgtgaccttt gcaggaatct
     2161 actttaagct gggcgattta atgagtatgt tttctctaca gagtaacgat tctcaaagtg
     2221 tgggaagtcg tatgatgaga aaacctcgta tgcttatgca cgctcacatg caccgtctac
     2281 agcggaaact tggacgctcc gtgactgctt taggggctgg gtctgccatt gccagtgcta
     2341 cgggtaaaaa aggacagtcg ggttcgggaa gttctgcgag tacacaagca gatcactctc
     2401 gaccaaacgg gcaggaaaaa tcaacactag gaaagcgtat cggtcaaacc atcggtacag
     2461 tagctgatac caaagacaga atggtagata ctgctggtaa tttgaaagaa caggttaagg
     2521 atttgccgac caatgcaaga tatgcagtat atcaaggaaa atctaaagtg acggataatg
     2581 tccatgattt aaccagaagt atttctcaaa ccaaagcgga taaagccagt ggacgcaaga
     2641 aacagcagga acaaagacgg aaaaccattg cggagcgtcg ctctgaaatg gaacaggtca
     2701 aacagaaaaa acagccagct tcttccgttc atgaaagacc agccactaag caagaacaat
     2761 ctcatgatgg acagacttca aggcaaacaa ctgtacaggc ttcctatagg gaatctcaac
     2821 aagccaaaca agagcgtcca acagttaagt ccgattcttc aagtttaaaa acggaacgtc
     2881 aaagtaatac agttcaagaa agaacggttc aaaagccagt aacttcaact gcaccaacag
     2941 atagagcttc acaacgttca atcacaaaag aacgtcagtc tactgttcaa agagtaccac
     3001 tacaaaatac aaaaagtaga ccaccaatca aaaccgccac cattaagaaa ggcagtaaga
     3061 aaccatgaag ctgaaaactt tagtgattgg tggttcggga ttattcttga tggtcttctc
     3121 actgcttctg tttgtcgcca ttttattttc agatgaacaa gatggcggtt tttccaacat
     3181 tcactatggc ggtgtggatg tttccgcaga agtgctggct cataagccta tggtagaaaa
     3241 agttgccaaa gaatatggaa ttgaagaata cgtcaatata cttcttgcga ttatacaggt
     3301 ggaatcgggt ggtacagcag aagatgttat gcagtcctcg gaatccctcg gtcttccacc
     3361 taattcattg agtacagaag aatccattaa gcaaggtgtg aagtatttca gtgagttatt
     3421 agccagtagc gaaaggcttg gtgtagattt agaatcggtt atccagtcct acaattatgg
     3481 tggtggtttc ttaggatatg tggctaatcg tgggaataaa tatacctttg aactggctca
     3541 aagtttctca aaagagtatt cgggtgacga aaaagtgtct taccccaatc ccatagccat
     3601 acctatcaat gggggctggc gatacaatta cgggaatatg ttttatgtgc aactggtaac
     3661 gcagtatctt gtcacaacag agtttgatga tgatacagta caagccatca tggacgaagc
     3721 tctgaaattt gaaggctgga agtatgtcta tggcggagct tccccgacta cctcttttga
     3781 ttgtagcgga ctgacacaat ggacgtatgg aaaagctgga atcaacttac cacgaacagc
     3841 acaacagcaa tatgatgtaa cccggcatat cccattattg gaagcaaaag ccggcgattt
     3901 ggttttcttt cattctacct ataacgctgg ctcttatatt actcatatcg ggatttattt
     3961 aggcgataac cgtatgtttc atgcaggcga ccctatcggt tatgccgact taacaagctc
     4021 ttactggcaa cagcatttag tgggagcagg acgaatcaaa caataagaaa ggacgattca
     4081 atgatgaaat tcggaaaaaa tcagaataca gaaaaacaga caccaaagga aaagaaacct
     4141 cgtgtctata aggtcaatcc tcgtaaaaag gttgtgattg tcctatgggt acttttagga
     4201 cttagtttca gctttgcgat attcaagcac tttacagcca ttgataccca taccattcat
     4261 gaaaccacca tcatagaaaa ggaatatgtc gatactcatc atgtggaaaa ttttgtagag
     4321 aactttgcga aagtctacta ttcatgggaa ctaaacgaca agtccattga taatcgcata
     4381 gaaaacctga aagcatatct gacagaggaa cttcaagctc tgaatgtcga tacagttcgt
     4441 aaagatattc ctgtatcgtc ttctgtaaaa ggatttcaga tatggacggt agaagcagat
     4501 ggcgacaatc aatttgatgt aacctacagt gtagaccaac tcattacaga gggggaaaat
     4561 acaaagaccg tccactctgc ttatatagtg agtgtctatg tagacagtac tggaaatatg
     4621 gtcctcatta agaatccgac cattactagc ataccaaaga aatcagacta taaaccaaaa
     4681 gctattgaaa gtgagggtac ggttgattcc attacaacca atgaaatcaa tgagttttta
     4741 acgacgttct tcaagctcta tcctacagcg acagccagtg aactttccta ctatgtgaat
     4801 gacgggatat taaaaccaat cggaaaagag tacatctttc aagaactggt aaatcctatt
     4861 tacaatcgta aggataatca agtcacggta tcgctgacag tggagtatat cgaccagcag
     4921 accaaagcaa cgcaggtatc tcaatttgat ttggtacttg aaaagaacgg gagtaattgg
     4981 aagattatag aataacaaat attggtacat tattacagct attttgtaat cacgtactct
     5041 ctttgataaa aaattggaga ttcctttaca aatatgctct tacgtgctat tatttaagta
     5101 tctatttaaa aggagttaat aaatatgcgg caaggtattc ttaaataaac tgtcaatttg
     5161 atagtgggaa caaataattg gatgtccttt tttaggaggg cttagttttt tgcacccagt
     5221 ttaagaatac ctttatcatg tgattctaaa gtatccggag aatatctgta tgctttgtat
     5281 gcctatggtt atgcataaaa atcccagtga taagagtatt tatcactggg atttttatgc
     5341 ccttttgggc ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc
     5401 tcatgttgat gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat
     5461 tacagaatta ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg
     5521 tcagagagga attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt
     5581 gaacatcata gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc
     5641 agttttagat ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg
     5701 tatattattt catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat
     5761 tgaccaaaat ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc
     5821 cgaaattgta atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac
     5881 cgaatctgaa caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat
     5941 gtccggtaaa tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa
     6001 ttgctccttg taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct
     6061 tatagaagtg ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg
     6121 cggaaatgtc ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct
     6181 ttatggcgga atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa
     6241 aattacagaa atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc
     6301 cggggaaatt gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac
     6361 aaagctattg ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt
     6421 tgaaccgagc aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga
     6481 cagtgacccg cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt
     6541 cttagggaaa gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga
     6601 gatagaacta aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata
     6661 taccattcac atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc
     6721 accgcttccg ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa
     6781 tcaatcattt caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata
     6841 tggttggaat gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt
     6901 tagtacccca gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa
     6961 agctggaaca gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata
     7021 tctttcacga gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt
     7081 gaaaaataat gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg
     7141 tagtgattta actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta
     7201 ccatgttact accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa
     7261 agtacgatat atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt
     7321 ttcttgttaa ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg
     7381 aattaattat gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc
     7441 tttgcgtttt atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg
     7501 atattgcaaa tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta
     7561 tgttaacctt ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca
     7621 aaaggttact cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg
     7681 gccattcttt cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg
     7741 catttccagc actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta
     7801 aagcatttgg tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg
     7861 gtggaatgat agcccattat attcattggt cctatcttct actcattcct atgataacaa
     7921 ttatcactgt tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt
     7981 ttgatatcaa aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa
     8041 catcatatag catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac
     8101 atatcaggaa agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta
     8161 tgattggagt tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg
     8221 ttccttatat gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta
     8281 ttttccctgg aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata
     8341 gaagaggtcc tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa
     8401 ctgcttcctt tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt
     8461 taggtgggct ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac
     8521 agcaggaagc tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa
     8581 caggtattgc aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac
     8641 ctatggaagt tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa
     8701 tcattgtcat tagttggctg gttaccttga atgtatataa acattctcaa agggatttct
     8761 aaatcgttaa gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg
     8821 aattcaaatc tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc
     8881 tctcttgtat cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca
     8941 ataaaaattt tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata
     9001 aaaattggca agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc
     9061 tgtcccttgc tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag
     9121 agggcaggtt tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat
     9181 ggttttggtc ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata
     9241 gtgtgttata ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg
     9301 ccacctaaaa aggagcgatt tacatatgag ttatgcagtt tgtagaatgc aaaaagtgaa
     9361 atcagctgga ctaaaaggca tgcaatttca taatcaaaga gagcgaaaaa gtagaacgaa
     9421 tgatgatatt gaccatgagc gaacacgtga aaattatgat ttgaaaaatg ataaaaatat
     9481 tgattacaac gaacgtgtca aagaaattat tgaatcacaa aaaacaggta caagaaaaac
     9541 gaggaaagat gctgttcttg taaatgagtt gctagtaaca tctgaccgag atttttttga
     9601 gcaactggat ccaggagaac aaaaacgatt ttttgaggaa agttataaat tattttccga
     9661 acgatatggc aagcaaaata ttgcttatgc aacagttcat aatgatgagc aaacccctca
     9721 catgcattta ggtgttgtgc ctatgcgtga tggaaaactg caaggaaaaa atgtgtttaa
     9781 tcgtcaagaa ctgttatggc tacaagataa attccccgag catatgaaaa aacagggttt
     9841 tgagttgaag cgtggtgaac gtggctctga ccgtaaacat attgagacag ctaaatttaa
     9901 aaaacaaact ttggaaaaag agattgattt tctagaaaaa aatttagcag ttaaaaaaga
     9961 tgaatggact gcttatagcg ataaagttaa atcagattta gaagtaccag cgaaacgaca
    10021 catgaaaagt gttgaagtgc caacgggtga aaagtccatg tttggtttgg gaaaagaaat
    10081 aatgaaaaca gaaaagaaac caaccaaaaa tgttgttata tcggagcgtg attataaaaa
    10141 cttagtgact gctgcgagag ataacgatag gttaaaacag catgttagaa atctcatgag
    10201 tactgatatg gcgagagaat ataaaaaatt aagtaaagaa catgggcaag ttaaagaaaa
    10261 atatagtggt cttgtagagc gatttaatga aaatgtaaat gattataatg agttgcttga
    10321 agaaaacaag tctttaaagt ctaaaataag cgatttaaag cgtgatgtga gtttaatcta
    10381 tgaaagcact aaggaattcc ttaaggaacg tacagacggc ttaaaagcct ttaaaaacgt
    10441 ttttaagggg tttgtagaca aggtaaagga taaaacagca caattccaag aaaaacacga
    10501 tttagaacct aaaaagaacg aatttgaact aactcataac cgagaggtaa aaaaagaacg
    10561 aagtcgagat cagggaatga gtttataaaa taaaaaaagc acctgaaaag gtgtcttttt
    10621 ttgatggttt tgaacttgtt ctttcttatc ttgatatata tagaaataac gtcattttta
    10681 ttttagttgc tgaaaggtgc gttgaagtgt tggtatgtat gtgttttaaa gtattgaaaa
    10741 cccttaaaat tggttgcaca gaaaaacccc atctgttaaa gttataagtg accaaacaaa
    10801 taactaaata gatgggggtt tcttttaata ttatgtgtcc taatagtagc atttattcag
    10861 atgaaaaatc aagggtttta gtggacaaga caaaaagtgg aaaagtgaga ccatggagag
    10921 aaaagaaaat cgctaatgtt gattactttg aacttctgca tattcttgaa tttaaaaagg
    10981 ctgaaagagt aaaagattgt gctgaaatat tagagtataa acaaaatcgt gaaacaggcg
    11041 aaagaaagtt gtatcgagtg tggttttgta aatccaggct ttgtccaatg tgcaactgga
    11101 ggagagcaat gaaacatggc attcagtcac aaaaggttgt tgctgaagtt attaaacaaa
    11161 agccaacagt tcgttggttg tttctcacat taacagttaa aaatgtttat gatggcgaag
    11221 aattaaataa gagtttgtca gatatggctc aaggatttcg ccgaatgatg caatataaaa
    11281 aaatta
//
LOCUS       DACCCS010000080        11238 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.084, whole genome shotgun sequence.
ACCESSION   DACCCS010000080 DACCCS010000000
VERSION     DACCCS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.084"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            83..1129
                     /gene="gmd"
                     /locus_tag="IVK82_002430"
     CDS             83..1129
                     /gene="gmd"
                     /locus_tag="IVK82_002430"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAP6393467.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            1209..2222
                     /locus_tag="IVK82_002431"
     CDS             1209..2222
                     /locus_tag="IVK82_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393468.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            2259..3203
                     /locus_tag="IVK82_002432"
     CDS             2259..3203
                     /locus_tag="IVK82_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAP6393469.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWKNGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            3271..4584
                     /locus_tag="IVK82_002433"
     CDS             3271..4584
                     /locus_tag="IVK82_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP6393470.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVISASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            4668..5570
                     /locus_tag="IVK82_002434"
     CDS             4668..5570
                     /locus_tag="IVK82_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAP6393471.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSVSFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            5575..7227
                     /locus_tag="IVK82_002435"
     CDS             5575..7227
                     /locus_tag="IVK82_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP6393472.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEKVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKADSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            7251..8117
                     /locus_tag="IVK82_002436"
     CDS             7251..8117
                     /locus_tag="IVK82_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6393473.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(8159..8350)
                     /locus_tag="IVK82_002437"
     CDS             complement(8159..8350)
                     /locus_tag="IVK82_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393474.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCGKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            8380..8919
                     /locus_tag="IVK82_002438"
     CDS             8380..8919
                     /locus_tag="IVK82_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393475.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGADVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            9047..9400
                     /locus_tag="IVK82_002439"
     CDS             9047..9400
                     /locus_tag="IVK82_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393476.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            9867..10343
                     /locus_tag="IVK82_002440"
                     /pseudo
     CDS             9867..10343
                     /locus_tag="IVK82_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            10343..10537
                     /locus_tag="IVK82_002441"
     CDS             10343..10537
                     /locus_tag="IVK82_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393477.1"
                     /translation="MAETRPKGYPKLKEYTPNRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            10543..10828
                     /locus_tag="IVK82_002442"
                     /pseudo
     CDS             10543..10828
                     /locus_tag="IVK82_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            10842..11208
                     /locus_tag="IVK82_002443"
                     /pseudo
     CDS             10842..11208
                     /locus_tag="IVK82_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
BASE COUNT         3153 a         2303 c         2855 g         2927 t
ORIGIN      
        1 ttatcgtgta ttcttgaaaa gtattagaaa ttacgagcca tttatatggt cttatcactt
       61 acataggaaa ggataatgta tcatgaaaaa agcgctgatt actggtatta ctggtcagga
      121 cggttcctat ctggcagatt tcctgctaga gaagggctac gaagttcacg gtatcactcg
      181 ccgtgcttct atttccaata ccgctcgcat tgaccatttg atgggcaaga tcacccttca
      241 tgacggtgac ctgtccgact catcttctct gattcgtatc atcagcattg ttcagcctga
      301 tgaaatctat aacctcgctg cacagagcca tgttcaggtt tcttttgatg ttcctgagta
      361 ctccggcgat gttgatgctt tgggcgttat gcgtattctg gaagcttgcc gtattctggg
      421 cctgacgaag aagactaaga tttatcaggc atctacttct gagctgtatg gcaaggttga
      481 agaagttcct cagcgtgaaa ccactccgtt tcatccttac agcccttatg ctgttgcaaa
      541 gcagtatggc ttctggatca ctaaggaata tcgtgaagct tatggcatgt tcgctgtcaa
      601 cggtattctg ttcaaccatg aatccgaacg tcgcggtgag aatttcgtca cccgtaagat
      661 caccctcgct gccggccgca ttgcagaagg cctgcaggac catctggaac tgggcaacat
      721 ggactccctg cgtgactggg gctatgcaaa ggactatgtt gagtgcatgt ggatgattct
      781 ccagcatgag actcccgagg acttcgtgat cgctaccggc gagcagcaca ctgttcgtga
      841 cttcaccgag aaggcattcg cagcaaatgg catgaccatt cgttgggaag gcactggcat
      901 tgatgaaaag ggctacgatg ttgaaaccgg caagatgctc gtttgtgtaa atccacagtg
      961 gttccgtccg actgacgttg acaacctgtg gggcgacccg actaaggcaa agaccgttct
     1021 cggttggaat ccgcagaaga cctcttacga ggaactggtt aacatcatgg caaagcatga
     1081 ccgtgaattg gcaaagcgcg aagctgcaat gaagaacgca gtcaagtaag ctgacattga
     1141 ataaacatat ctgtttctat ctttccagtc acggcttcgg acatatcgcg cggaatctgc
     1201 ccattattat ggcaacactc tgccgatgcg aagcgaagat tacactggtt tgcggcgcaa
     1261 atcatctgga ttttgcaaag cgcaatttgg agcaggaact gcccaaagag ctgatagacc
     1321 gcgtgcgcta tcgtccaata aacacagata tcggtctgat taaccgtgag ggcaccctac
     1381 tggtggacac gggtaagctg accgagatct gtgaatattt tttggcaaag gttccttctt
     1441 tggcaaagac agaagccgaa tggctcaagc gagaagctgt cagcgcagtg ctttgcgata
     1501 tgccaatctg ggcaatggat gcctgcgata tggcaggagt acctttcctg tatgtaggaa
     1561 acttcacttg gacagagctg tatcgcgaat ttttacccga acatatttgg aaagaatatg
     1621 ccaagcgtta cggtaaaatt cagcacacca tgctttatgc gctaaataac gaagaaatgt
     1681 tggaatttct ccaaaacagc gaaaaaacac cgacttccgt tgtggcgcgt ccgattcata
     1741 acgacgaagt gcaacgcatt aaatcacagc atgaaaaacc gcctgtattt gtcgcactcg
     1801 gtatgtccgc gcggttcaaa cagccaattg atgtcagtca cgcgccgtat gatttcatta
     1861 caacgctagg tgttccgctt gtgggagaaa acgtaaccgt tatttctaac agcaccccca
     1921 atacacagga ctatatagcg gcggcagatt acgttattac aaaggctgga tggggaactg
     1981 ttgcggagtg tattctcgca cagcgtccga tggcactgtt cgcccgcgat acagttttgg
     2041 aagatagaac aactatttgt aaattgcaaa aactgggtct ggcaatttcc gtcacaccgg
     2101 aaaacctcca ccgcgtagag cgggtgattg aggacctcaa acagctagac acctcaaatt
     2161 ttgatacatt ttatgatgct gcgagcgaaa tcgcagacaa attgatttct ctcatgcaat
     2221 gagagatgct ctaaatttag aaaggagcaa gcattattat gatggaaaaa gacgcaaaaa
     2281 tctacgtagc cggtcatcgt ggcatggctg gttccgcaat cgtgcgcgag ctgaaccgtc
     2341 aggactacaa caatattatc acccgcaccc ataaggagct tgacctgtgc cgtcaggacg
     2401 ctgttgaagc atttttcgca caggaaaagc cggattatgt atttctcgct gctgcaaagg
     2461 ttggcggtat tatcgcaaac cagaacgctc tggcagactt catgtatgaa aacatgattc
     2521 tggaaatgaa cgtcatcaac tccgcatgga agaatggctg taagaagtta cagttcctcg
     2581 gttcttcctg tatctatccg cgtatggcac cgcagccgat gccggaaagc tgtctgctga
     2641 caagtgagct ggaaaagacc aacgaggctt atgcactggc aaagatttct ggcctgaagt
     2701 actgtgagtt tctaaacaag cagtacggta ctgactatat ctctgttatg cctactaacc
     2761 tgtatggacc taacgacaac tatcatccta ctcacagtca tgtgctcccc gctttgattc
     2821 gtcgcttcca tgaggctaag gaagcaggtc tgcctactgt tacctgctgg ggtgatggtt
     2881 ctcctctgcg cgaattccta tatgttgacg atctggcaaa cctgtgtgtg ttcctgatga
     2941 ataactatag cggtgacgag actgttaatg caggtactgg caaggaactg tctatcaagg
     3001 aactgaccga gatggtagca aaggttattg gttacgaagg cgagattctg tgggatacca
     3061 gcaagccgaa cggtacacct cgtaagctgc tggatgtttc caaggcaacc aagctgggat
     3121 ggacatataa gactgagttg gaagatggca tccgtctgtc ctacgaggac ttcctgaata
     3181 acccgatgcg tgctgaacgc taatttaccg aggttgctgt gatttttgag gattgcagca
     3241 gcctctttct tataaaaagg agagatcatt atgaatattg ttttactgtc tggcggttct
     3301 ggtaagcgcc tttggccgct gtctaatgac atccgttcca agcagttcat taagattttc
     3361 aaaactgaag atggatatga atccatggtt cagcgtgtct accgccagat taagaaggta
     3421 gataacgacg caactgttac gattgcaacc tctaagacac aggtttctgc tatccataac
     3481 caacttggtg aggatgtggg tgtatctgta gagccttgtc gccgcgatac cttcccggca
     3541 atcgcattgg ctaccgctta tctggtagat gtgatgggca ttgatccaga agagtccgta
     3601 gttgtttgcc cggttgaccc ttacgtagaa gatgactatt ttgaggccct gaaatctctg
     3661 tccgatcagg ctgacaaagg cgaagctaat ctggtgctca tgggtattga accgacttat
     3721 ccttccgaga agtacggcta catcattcct gagagcgcag agcagactgc tttcgtaaat
     3781 accttcaagg aaaaaccttc tgaggctgtg gcaaaggaat atatcgcaca gggtgcactg
     3841 tggaacggtg gtgtgtttgt ctacaagctg aagtatgtca tggagcgtgc acatcagctg
     3901 attgactttg tagattatca ggatctgttc aacaaatacg atactctgac taagatttcc
     3961 ttcgactatg ccgttgttga gcatgaggac aagattcagg tacagcgttt tgctggtatg
     4021 tggaaggatc tggggacttg gaatactctg accgaaggta tggaggaatc cattatcggt
     4081 aaaggtgtta tgaatgagaa ttgcgatggc gtacacatcc tgaacgaact ggatgttcct
     4141 gtgttggcta tgggtctgca tgatgtggtc atctctgcat ctcgggaagg tattctggtt
     4201 tccgataagg aacagtccag ctatattaag cctttcgttg acaagttcga gcagcagatc
     4261 atgttcgcag agaagtcttg gggtagttat aaggttatgg atgtagaagc agagagcatg
     4321 accattaagg ttactctgaa tgctagtcac tccatgaatt atcacagcca caagaaccgt
     4381 gacgaggtgt gggtggttct ggccggtact ggtaagacca tcgtggatgg atatgagcag
     4441 gttgttaagg ctggcgatgt gatcaccatg agtgctggct gtcggcatac cattatggca
     4501 gatactgagc tgaagctgat tgaagtacag cttggcaagg atatcaatgt taaagataaa
     4561 cagaaatttg agttagagta ttaaaaaacg gaggagggct cgttatacag aaaccgtttt
     4621 tattgaagcc ttccgcaaag gattatctgt gggggggaac cgcctgaatg atgaatttgc
     4681 aaaaggaatt gagatgtctc ctcttgcgga aacttggaat gctctacgca cccggatgga
     4741 ccgagctttg ttgtgtctgg tcagagtatc aggggatgac cttgacggag gtgttgcaag
     4801 aacacccaga gtttctggga acgcatccgc agaccaaagg agaattgccg atcctgatca
     4861 aattcattga tgcgaaaaag gatttgtctg ttcaggtaca tcccacagat gaatatgcaa
     4921 ttgaaaaaga aaacgggcag cttggaaaga cagagatgtg gtatgtactg gatgccagct
     4981 taatctatgg gctgcagaaa ataacagaag aagctgtgat ccgtgaagcg attgctcagg
     5041 gaactgttga gcgttatctt cagaaggttc cggtcaaaaa gaatgatgtg ttctttattg
     5101 aggcgggaac gattcatgcg attggtgcgg gtgcactaat tgcagaaatt caggaaaatt
     5161 ccaatctgac ttatcgtctc tacgattatg accgtgttga caaaaatggt aaaaagagag
     5221 aacttcatgt agacaaagcg ctagaagtag cgaacttgaa agggttgtca gaaccgaaac
     5281 agccgcttcg tgttctaaaa tatcgtgctg gtgtagctgc ggaattgctt tgcagatgca
     5341 agcatttcga ggtctctcgt atgattgtca acactgagag aagacagacg gtacactatc
     5401 gtgcggattc agtcagcttc cgtgtgttgc tgtgtgtcaa tggctgtggt accgttcgat
     5461 ttgataatga atcgttggac atctataagg gggactgcct ttttgtgccg gccgattcta
     5521 ctgaaatcag cattcacggg tcgattcagt ttctggatgt gagaggataa taatatgaat
     5581 tataaagtag aatatgagcg ttggctgaat catgccaatg cagatattat agaagaactg
     5641 cgttcctatt ctgagaagga cattgaggac tctttctata aaaatttgtc tttcggtact
     5701 ggtggtctgc gtggtaccat cggtgcaggc actaatcgca tgaacgtaca caccgtaggt
     5761 aaggcaagcc aaggtctggc agactatctg aataagaact atgaagccgc ttccgtggtg
     5821 gttggctatg attcccgtat caaatctgat gtgtttgcaa agactgccgc agacatcttt
     5881 gctgcaaatg gtattcaggt gcacttgtgg ccagaactgc ttcctgttcc tacagtatct
     5941 tttgcgactc gttatctgaa ggcagatgcc ggtgttatga ttacggcatc tcataaccct
     6001 tctaaatata acggctacaa ggtatacggt gcagacggct gtcagattac taccgaggct
     6061 gctactgcaa ttctggcaga aatcgaaaag ctggacatct ttgctgatgt aaagatggat
     6121 ggcgatgctt ctaagattgc atccattcag tcggaagttc tgactgcttt catcgaagaa
     6181 gtgaaggggc agtccgtcct gtttggcgaa aaagtggaca agaatgttgc tatcgtgtat
     6241 tctcctctga atggtaccgg tctggtgcct gttacccgca ccctggcaga gatgggatac
     6301 agcaatgtga ccgttgtgga agaacagcgt ctgccggacg gtaatttccc gacttgtcct
     6361 tatccgaacc ctgaaattaa ggaagctatg tctctgggta tggagtatgc caagaagtac
     6421 aatgcggatc tgctgttggc cactgaccct gactgcgacc gtgttggtat cgcagttaag
     6481 agcgcacatg gtgactatgt tctgttgtct ggtaatgaga ctggtctgct acttctggac
     6541 tacatctgtt ctcagagaat taagcatgga aaaatgcctg ctgaccctgt gatggtcaag
     6601 accatcgtca ccatggattt gggtgaacgt atcgcatcca actatggtgt tcgtaccatc
     6661 gatgtgctga ccggctttaa gtttatcggt gagcagattg gtatgttgga aaaggctggt
     6721 aaggctgaca gctatatctt cggctttgag gaatcttacg gctatctgtc cggtagctat
     6781 gtccgtgaca aggacggtgt taacgctgct tatctaatct gtgaaatgtt ctcttactac
     6841 aagactcagg gtatcagcct gctggataag ctgaatgaga tttatacaaa gtatggctat
     6901 tgtttgaaca ccctgcattc cttcgagttt gacggtgcgg caggttttac caagatgcag
     6961 gaaattatgg ttgaattcca caagggtctc gatataattg gtggtaagaa ggttattgaa
     7021 actttagact acagtaaggg cctgaatggt ctgccgaagt ctgatgtttt gaagtttatg
     7081 ctggaagata actgctctgt tgtggttcgt ccttctggta ctgagccgaa gctgaagacc
     7141 tatatcagcg tcagcgcaga gaacaaggaa gccgccgaag ctatggaagc tattatggct
     7201 gctgaactgg cggaaatgtt cgagtaagaa aagaaaggag aaatcttgta atggaggaac
     7261 tgttttctaa cgctttgaaa tcggtgaagt caaatcgtgt gctgtacacg gcatcgttct
     7321 ttgcaagatc ctctcttttg tatttgcagg aaattggaca gctgactgct ctgaaggcac
     7381 atacatctag gcgtacagga ttgtcatctt acctttgttt cctggtagaa gttggttcag
     7441 gcacattgga gtataagggg cagaaatata cgcttcaagt aggagatatg gtgtttattg
     7501 actgtttgaa gccgtatgtc catgagtcat cagaagatga tttgtggact ttgcggtggt
     7561 gccattttaa tggcattggt atgacggaag tctacaaaaa ataccttgaa cgaggtggtc
     7621 aaccggtctt taaaacatca aaaacagata agtatgctga cttgctgagg agccttcata
     7681 tggtggcaga gggacaggag tatgtccggg atatgaaaat caatgttcta ctcagtgaac
     7741 tgctgatgca tttgatggaa gattcttgga atcctgacaa agtgccgaat ttctctacaa
     7801 aaaaaatgga attgcagaac ataaaacaat atctggatga ccattacacg gagaagatta
     7861 cgctggatca gttgagtgca gagtttttta tcaataaaca gtacttagct aagatttttc
     7921 gtgaaactta cggtacaact gttgtcggtt atctgaatca gaaacgaatc acacaggcca
     7981 agcacttgct acgctttaca aatctaagta tggaagagat tgcaacaaaa attggcataa
     8041 cagatgcaaa ctatttttcc agaacttttt tcaaggtgga aggtgttcgt cccagcgaat
     8101 tccgcaagat gtggtaataa aaagccggcg gtgttgagcc gccggtcagt acctgtcgtt
     8161 aacccgcatt caaagcttta atctcagctt tgatttcagc acgaacacga tagtaatctt
     8221 cagtttcgat ttcggtatcc agccgagttg ctgtctttcc gcagtcagga caacgctctg
     8281 gctgttcgtc ggcagggaag ctgtagtggc aggaatcaca ggtgtaaatg tgatcgtcac
     8341 gtataatcat cacctaccaa tccagtgctt agatttttca tgcaattact attatatcga
     8401 attggtgatt ccacaaaaat ccgcttcttg ataaatgtac gattcagagt gatatatagt
     8461 actgtactgc caaagggaat accacaacgg cagaaatgga tgattcacat ggaagaaatt
     8521 atgcagatga ttttagggaa cgcaaaattc cagctcggac aggtggtttc gacccctgct
     8581 gtattggaaa ctttcaaccc cctgcagacc gcaaacggca ttcccgaagc cttgagcaac
     8641 ctttggttta gacaggaaat tagtattgcg gagatcgagg acgcagttct ggatgccaag
     8701 aacggcaagg agctagcaaa cagcctcaac cgcatcaagc ttttccgcaa gttcaccctc
     8761 gaccgagagc tggacggcat ggtcagactg aagggcgcgg acgtctggaa gaacatcagc
     8821 tacctcaagg tctacaccgc cacgccgcct aagaaaccga cccccccaag gttccagtcg
     8881 aacggctgga gccttttctt ctctcataaa tcggcataat gtatacaaat acagctccaa
     8941 atgattgtgt agtatatgcc tgtggattga cttgatatag tcgagagact atggcaatat
     9001 acacacaacc aaaggggaaa atcccaaaaa caaggaggaa acgctaatgg caatgagcga
     9061 aaaggcaaag aaatatttga aggagatcaa gggtgcaaag accatcccgg agctgaagga
     9121 tgtggagatc gcaatcaagc gagacggtat ccttgcttgg gcagagttca ccaagttgaa
     9181 cgaggcggtg gaagagaaga aggtcgccct tcgcaagaaa aagcaggaga ccagccttca
     9241 ggagatcctt ttctgggcct acaaaaagga atcagacaag ctgctgaaga tgatggacga
     9301 gggagcagac aaggatgcca tccagatgca ggtccagcgt tacgacagca tcggccagat
     9361 cattgcagag gcagacctcg aagatgagta tgaggtttga gaatacagcc tgaaccacta
     9421 agaaccaaat accgaaaggg acttctgaaa cagacagagg tccctttctt ttgctcactt
     9481 tggcggtcaa ttatgaacca aaaaccagtc gaatgattgt gtacattatg gctttcaaac
     9541 gacttgataa taatcacttt tagagtgata tatgtactac aaaaaacaaa agactggaga
     9601 aaacaacaga tgagcgaata cagaatcatt tatatcagcg acgaggacgg cggcgaggtg
     9661 gaaggatgtg gctacacggc tacgatggtt atgatgcggt cagaaacttc ttggaatttg
     9721 cctgcggcat cttgaaaatc ctgcgggtgc gattcatcag aagagtggac tgaaatacaa
     9781 gttagcagat agagaagaga gtcaggtttc gacttggctc tgttctctgt ctactggcag
     9841 aaatacattg aagggaggtg acgactgtgg caaccagagg aagaaaatca accccgacag
     9901 ccatgaagaa gctggaggga aatccgggca atcgcccact caatgcaaaa gagtcaacgc
     9961 cggaaaagaa ggcaccgacc tgtccgaagt ggctggaacc agaggcaaag aaggaatgga
    10021 agcgtctggc aaagcagatg gaggccatcg gaatcttgac agatgtggat atggctgcct
    10081 ttgccggtta gtgccaagcc tacgcccgct ggaaggaagc cgaggaattc attacccagc
    10141 acggtatcat cgtgaaaacc ctatccggct attggcagca ggtaccacag gtgtttattg
    10201 cccagaccta tctgaaaatt atgaaccgat tttccgagca gttcggtctg acgccttcgt
    10261 ccagaagccg tatcattgcc ggtgatggcg gtggtgtcgg agcagaagat gcgatggagg
    10321 ctctgttggg aggtggtagc tgatggcaga aaccagacca aagggctatc ccaaattaaa
    10381 ggaatacaca ccaaaccgat tcatgctttc ggaatgtcac tacgacaaag ccagagcgga
    10441 tcgggcggtg aactttatcg gacagctccg acacaccaaa ggcaaatggg caggcaaccg
    10501 cttctggctc ttgccttggc aggaacagat catctagtgt aatctcgctt aactgtatta
    10561 acggacgtag cgactttatt aagagtgttc atggtaggag cattagttgc aacaatgatt
    10621 gatattaaat ttttatggta ccaacgattg gcgatgtgac attgaatatg caaaactata
    10681 tcgatatgat attatctaaa atcttactcg ctgggatcgt cgaggtgttt attggttgtt
    10741 aggaaagaaa aatatgaccc aactcgagcg atttttatcg tattacttgt gtgtggcttt
    10801 atctgcattg tgtgtgattt cgaaataagg gagagccaaa aatgagtaga aaattgatat
    10861 tgattagtca cggaaaattt tgtgaggaat tgaaaaaaag tacaaaaatg attatgggac
    10921 ctcaagagaa tatctacacg ttacctttat taccagaaga tggcactgaa acatacagac
    10981 aaaagctctt actgtcatag attcgttaaa tgattttgtg gtactatgcg atcttttagg
    11041 cggtacacct actaatatca tttctaaact gataatggaa ggacaacaga ttaaacttta
    11101 tgtcggatta atcttcctat ggtcattgaa tttatcaact cgcaagtgat tggttcagag
    11161 cccgagtttg ttcgtgcagg atgtgaaagc attgtatctg tcaactaatt aattagtgga
    11221 atagtagaag atgaagag
//
LOCUS       DACCCS010000081        10510 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.085, whole genome shotgun sequence.
ACCESSION   DACCCS010000081 DACCCS010000000
VERSION     DACCCS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.085"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="IVK82_002444"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="IVK82_002444"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP6393478.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="IVK82_002445"
     CDS             1085..2005
                     /locus_tag="IVK82_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6393479.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="IVK82_002446"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="IVK82_002446"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP6393480.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="IVK82_002447"
     CDS             3311..4789
                     /locus_tag="IVK82_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6393481.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5066..7126
                     /locus_tag="IVK82_002448"
     CDS             5066..7126
                     /locus_tag="IVK82_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393482.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7209..7778
                     /locus_tag="IVK82_002449"
     CDS             7209..7778
                     /locus_tag="IVK82_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6393483.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7953..8759)
                     /locus_tag="IVK82_002450"
     CDS             complement(7953..8759)
                     /locus_tag="IVK82_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393484.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8897..10411
                     /locus_tag="IVK82_002451"
     CDS             8897..10411
                     /locus_tag="IVK82_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP6393485.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3726 a         1752 c         2071 g         2961 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct
     4981 tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga
     5041 caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa
     5101 acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat
     5161 gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc
     5221 aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat
     5281 cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc
     5341 ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac
     5401 attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga
     5461 tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga
     5521 tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt
     5581 tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga
     5641 aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg
     5701 gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc
     5761 aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc
     5821 tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt
     5881 tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg
     5941 gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa
     6001 aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc
     6061 gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca
     6121 tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt
     6181 gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg
     6241 gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca
     6301 ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta
     6361 tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat
     6421 aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa
     6481 ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac
     6541 aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc
     6601 tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag
     6661 acaagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg
     6721 gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc
     6781 taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga
     6841 aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga
     6901 agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga
     6961 ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc
     7021 tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg
     7081 ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc
     7141 tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg
     7201 atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc
     7261 aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag
     7321 aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg
     7381 aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga
     7441 tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa
     7501 aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag
     7561 gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata
     7621 cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata
     7681 aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg
     7741 aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc
     7801 agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact
     7861 atcggataac taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg
     7921 tcactgcccc ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa
     7981 ttcttgatga acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg
     8041 aaaacaactg ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg
     8101 tctaagtaac cattttggat gattgtttta tctgttttac catctgcatt ttgactttca
     8161 atgattgtcc gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc
     8221 acactattga tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct
     8281 gctgaaaatt ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca
     8341 actgctagta ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca
     8401 agggaaaagc cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac
     8461 attgaaaaat agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa
     8521 gataacaaaa tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa
     8581 taagggattt caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg
     8641 ttccagacct gatccactag cataataacg acgatgatac gtaataaaat atagattccg
     8701 tttgtgattc tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata
     8761 aaataactcc ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat
     8821 atgacttaaa gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga
     8881 gaaaaggggg atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct
     8941 tgctatgagc gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa
     9001 ggaatctgcc gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat
     9061 gaaagaatct tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat
     9121 cgaagcaaaa gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg
     9181 aaacacatct aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga
     9241 aggaatcaaa gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac
     9301 gaatgataaa gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct
     9361 agatcaaaac ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag
     9421 gacacatcgt ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa
     9481 taacgtccat gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa
     9541 aaatggggaa acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt
     9601 caccggaaaa gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga
     9661 aaaatatcgt cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga
     9721 tggtatcacc atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat
     9781 tcatccaact gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg
     9841 agcaatcttg gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa
     9901 agtatctgca gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg
     9961 cgtgcgtgat cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg
    10021 aaagacaatg gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac
    10081 agttgatgga tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac
    10141 agccatggaa catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat
    10201 ccattacacg atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa
    10261 ccagccaatt agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa
    10321 ccggatcggt ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca
    10381 agcagctcga tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga
    10441 aatcagcgtt ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt
    10501 tgtgaaaaag
//
LOCUS       DACCCS010000082        10290 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.086, whole genome shotgun sequence.
ACCESSION   DACCCS010000082 DACCCS010000000
VERSION     DACCCS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.086"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(109..351)
                     /gene="acpP"
                     /locus_tag="IVK82_002452"
     CDS             complement(109..351)
                     /gene="acpP"
                     /locus_tag="IVK82_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP6393486.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(473..1474)
                     /gene="plsX"
                     /locus_tag="IVK82_002453"
     CDS             complement(473..1474)
                     /gene="plsX"
                     /locus_tag="IVK82_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP6393487.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1523..3559)
                     /gene="recG"
                     /locus_tag="IVK82_002454"
     CDS             complement(1523..3559)
                     /gene="recG"
                     /locus_tag="IVK82_002454"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP6393488.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3676..5358)
                     /locus_tag="IVK82_002455"
     CDS             complement(3676..5358)
                     /locus_tag="IVK82_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP6393489.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5410..5772)
                     /locus_tag="IVK82_002456"
     CDS             complement(5410..5772)
                     /locus_tag="IVK82_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6393490.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            6042..6230
                     /locus_tag="IVK82_002457"
     CDS             6042..6230
                     /locus_tag="IVK82_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP6393491.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6468..9137
                     /locus_tag="IVK82_002458"
     CDS             6468..9137
                     /locus_tag="IVK82_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP6393492.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(9592..>10290)
                     /gene="truA"
                     /locus_tag="IVK82_002459"
     CDS             complement(9592..>10290)
                     /gene="truA"
                     /locus_tag="IVK82_002459"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP6393493.1"
                     /translation="GFQKQPNGRTVQEEVEKTLQKMANGKEITVFGSGRTDAGVHAIG
                     QVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHARYLVKEKTYQFRVDIG
                     KPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCASGSSIEDKIRTIYEAKM
                     EVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDSIPDIIAKKDRNAAGPT
                     AHPEGLYLYEVKYE"
BASE COUNT         2811 a         2220 c         1852 g         3407 t
ORIGIN      
        1 ttatcgccag aattatcaga aaaatgactt ttacgtgtca aaaagattaa aaaaagaagg
       61 ataaaaagct gtatgcagct ttttatcctt ctttgctttt atttgaaatt atttaagatg
      121 gctgctgata taatctactg cgccaccgac tgtttcaatt tgttcagcgt cttcatctga
      181 aatttccgta ccgaattcat cttcaagctc taaaacgaat tccataatac taattgaatc
      241 agcttttaaa tcatctttga tgttcaattc atccgtcact ttatcggtat ctatctcaaa
      301 atgatttgaa ataattttcg ctactttgtt aaatacttct tcgcgagtca actttttcac
      361 ctccaagaat catacacttg tcaattacct acaccatggc taattttact ggcatttttc
      421 aggaatgtcc accttttttt caatggaacg gatcgcttca gatatccaaa tactattctt
      481 cgcttttttc aaattgttcg actaattgtc caactacatc tgtctcaagc attgtgtgga
      541 tttggcggat cgtatagcga acagcatcgg ggcctgttgc tccatgagtt ttaatgactg
      601 gtgctttcaa gccaaacaaa acggcaccgc catgtttgga ataatccatt tctgctttca
      661 aagaacgtaa gccgtctttt aaaagcatag cgcccatttt tcctttgatc ccttcattta
      721 gaatagcaga ttttaataga ttcatcatat tcattgctgt tccttcaata gatttcaaaa
      781 cagcgttgcc tgtaaatcca tctgtgacca ctacatcagc cacaccattg agtagctcac
      841 gagcttctac gtttccaata aaattcaagc ttgtttcatt ctgaaggagt tcataggctt
      901 ttttcgtcac ttcactacct tttgttgctt ctgttccgtt gtttaagagt gcgacacgag
      961 gacgtccgat acctcggact ttacgtgcat aaaaagaacc taaaataccg tattgcaaca
     1021 aatgttctgg tttgttttct gcgttagcgc caagatccag catatcgaaa ccggcacctt
     1081 ctttccctac tactggcaag gtagacatca agccagggcg ttcgatattt ttgatacgtc
     1141 ctacgatgaa caaccctgct gctagtagcg cgcctgtatt ccctgctgaa aatacagcat
     1201 ctgcttcacc gtttttcact gcttgtgcag caaggaccat tgatgcagtc ttttttcgtc
     1261 gaattgcttt tactggctca tcatcactat tgattttttc gtctgtatgg ataatcgtga
     1321 tgtttttttc atttgttaag tatttcttga tttctgcttc ttttccgtaa agtaagaatt
     1381 ctacttctgg aaaatcttgc tgcgctaaag ccacgccttc tacaattgct tgaggtgcgt
     1441 gatctccgcc cattgcatct acggcaattt tcattgtttc cactcctttt tccgtcatca
     1501 gtataaagta tagcacagtc tgctaatcga aataacgatt atcctgattt tctatttgga
     1561 ggctttgcgc taaaggctga tactcaggaa gcatctgcca atcctttatt tgccaaaggg
     1621 ctgcagcctc ttctctggct acctccaaaa catttccatc cgtcaccaag tcagcaatcg
     1681 caaattgagg cagaccagat tgacggaagc caaatacttc accaggacca cgtaattcca
     1741 gatctttttc gcttagtaca aaaccattgg tggtctctgt catgattttc atccgttcca
     1801 cacctagttc atttttagga ttagcaacta agatacagta ggaagcatca cttccgcgtc
     1861 caacacgtcc acgaagttgg tgcagctgtg ccaatccaaa tcgatccgca tccatgatca
     1921 gcatgacggt cgcgttcggc acgtttaccc ctacttcaat tacagtagtc gaaactaata
     1981 tctgcatctg gttgtctttg aaggcttcca tgattacttc tttttcctga ttcttcattt
     2041 taccatgtaa caagccaact tcatattttg gtgcaaatag ttcacgaagt ttttcataga
     2101 tttccactgc attttttaca tctaatgctt ctgattcttc aatcagcgga cagatgacat
     2161 acatttgatg tcctcttgcc agttcttttt tcgtccaatc cagtacactg tccaattgag
     2221 gtgttcttac ccagcgtgtt tcgatcggga tacgtccagc tggaagttca tcaatgatag
     2281 atacatccat ctcaccatat gctgtaatag ctaatgttct aggaatcgga gtcgctgtca
     2341 taaataagac atctggtcgc cagccttttt cacgaagtac tttccgctgg ttcacgccaa
     2401 aacggtgctg ctcatccgta ataactagac ctaaattctg aaattcgact ccatcttgga
     2461 tcaatgcatg tgtgccgatc acgacgttaa tttccccttg ctctagtttt tcaaggagct
     2521 cacgtcgttc ttttgctttc gttgaccccg taatcaatgc gatggttact tcattgggat
     2581 caaacagctg ttgcaagctt tccatatgtt gttgtgccaa gatttctgta ggcaccatca
     2641 acgcaccttg gaaacctgct gtaacggtag cgtataacgc aatcgcggca accactgttt
     2701 ttccgctacc tacatctccc tgcaaaagtc gctgcatatg ctgcggactg cgaagatccc
     2761 gacagatttc attcgttaca cgtttttgtg catttgtcag ttcaaaaggt agcttttgag
     2821 taaattgttt caatttttgg acatcataaa ggatctccaa accattcgtt tctgcttttt
     2881 ctgctttttt caatccttga agacgcattt gaaataaaaa gaactcttca aaaacgacac
     2941 gtcgtttggc ttgatggttt tcttcgtggt cttttggaaa atgcatactt cttacagcag
     3001 aagctcggtc aagcagtcga tatttctcca caaggttgtt tggcagattt tcttcaatca
     3061 atgtgccgta ttcttgaaac ccttttttga ttagatcaac taatgtcgtt tgacgaattg
     3121 atttgttgac gtgatatatc ggcgaaaagt catccattgt ttgagaacct aaaatcttca
     3181 tcccgttcaa ggctttacgc tttgcatccc atttaccgta tacggcgatt tcctccgata
     3241 aaatgacttt ttcttttaga taaggttgat taaaaaaaga aacgttgaac acatcatgtt
     3301 cttgcatcat acgaaattgc aatcggcttt ttttataacc aaatcgattc atcacaggag
     3361 gcgagactac gattcctttc aaagtaacct tttcttgatc ctggatctcg tttagatttc
     3421 tttcctgaat atcttcgtag cgaaaaggat aataagtcag taagtcttca atcgtttgga
     3481 tgcccaagct ggcaagacta gctgctcttt tttctccgac tcctgccagc atagtgattg
     3541 gatcatctaa agaacgcata aatttttcct ccctccttta acaaaaaaag gacttggtca
     3601 gcgatcatac ctgatcacta accgaaggcc ttttatgaat gaatactgtg ttagaaagat
     3661 attctgacac agaaattatt cagcagaaaa tagataagga tagacaggct gatctccttg
     3721 gtggatctcg atttccaaat caggatgttt ttctgttagt ttctcagcaa attcatttgc
     3781 ttgttcaact gttccttcat cacctgccaa gatcgttacg atctctgtgt cctcattgat
     3841 catttgttct agtgtactta acgctgttgc tgtgatatct gggttagaga tcaggatttt
     3901 tccatcgatc atgcccaaaa agtctccttc tttgatctct acaccgtcaa ttgaggtatc
     3961 tctgatcgcg tgagtgactt gaccgctcac aacgctagac aacatttctg tcatcgtttc
     4021 tttgttttct tctaaagaag cttgatcatt gaatgaaagc atagctgtca atccttggga
     4081 aatcgttctt gaaggaacaa ctgctactgg gatatctgct acttctgctg cttgatcagc
     4141 tgccatgaag atgttcttat tgtttggcaa gacaatcact ttatctgcgt ttacttcttg
     4201 aatcgcttta acaatgtctt ccgtacttgg attcattgtt tgtccaccac tgataacata
     4261 attagcgcct aaactttcaa acagctcttg gacgccttta ccagcagcga tcgctacgat
     4321 accatacggg atacgtggta tttctttttt cgcaaactcg ttcacttctt catcgtgttc
     4381 caaaatcgtc tcatgctgca agcgcatgtt gtcgactttg attttgacta atgaaccgaa
     4441 tttttgtcca taattcaaga cttctcctgg gttttctgta tgcacgtgga ctttgatgat
     4501 ctcgtcatca ttgaccacaa gtaaagaatc tcctatacca tctaaatagt tacggaattc
     4561 ttcgtaatcg aatgtactat cgactgtagg tccttctccg atttttacca tgatttctgt
     4621 acagtaacca aaatggatat cttctgtcgc catttgacct tggacactgc gatgatgttc
     4681 tgcattgacc atatcatcca tttctgctgg agaaggttca tatgtttcat ccgcttggaa
     4741 ttctccattc aatgcactta aaaagccctc ataaacaaac agcagtcctt gccctccact
     4801 gtctacgaca ccgacttctt ttaggactgg aagcaagtct ggagttttag ctaaagcacg
     4861 ctttgctcct tttacaacag ctgtcattac ttcaacgaca tcatcagtac tttttgcttt
     4921 cttttctcca gctttcgctg cttctctagc tactgtcaaa atcgttcctt ctactggttt
     4981 cattactgct ttatacgcag tttcaactcc atgagtgaag gctgcagcaa gatcagaagc
     5041 atttaatgag acaacatctg gaatttgctt agaaaatcca cgaaacaatt gagacaaaat
     5101 aacgccggaa tttccgcgcg cacccatcag caatcctttt gaaagcattg ctgctaattc
     5161 tcctactttt tctgaggttg aatctactac tgccttagct ccactagtca tagatagatt
     5221 catatttgtt cccgtatcgc catctggcac agggaataca tttaatgaat tgacgtattc
     5281 tgcatttact tgcagacgat tcgcacctgc ctggaccatt tcctggaact ggcttgcact
     5341 tatttctgtt acactcacct agataatcct ccttcagatc gactgctgag ttaccagcca
     5401 gtctcctgcc tagtctggca gtacacgaac accttgtaca aagacattaa ctgaattggc
     5461 agttacgcct aataatgcct ctagattgta tttcactttt tcttggacgt tgcgggaaac
     5521 ttcagagatt ttcgtaccat agctgataat cgtataaaca tctacagcaa taccattatc
     5581 ttcttggcgg acaactactc ctcttgagta attttcttta cgcaaaatct cagtaatatt
     5641 atctttaatt tgatttttgc tggccattcc tacaacaccg aacacatctg tcgcggcgcc
     5701 accaacaact gttgcaatta catcattggt aatttcaatg gttccagctg acgtttgaat
     5761 ttttacagcc atgaatgagc ctccttaacg ggtaaaaaac tcccattttt cttctatcat
     5821 attttatcat agcgcaactt gaaataaaag gcagttaccc aaagtactca aaaaccttgc
     5881 aaaatttcag gtataacctt gtttaattta caattattct tctcaattgt caattgtatc
     5941 tatgaaaatg tacttgcaaa ttcccattct atatgatatg ttagtctggt atgagctgaa
     6001 ttattctatt gctccaaatc aaggcaaagg aggaaattac catggcaaaa gtttgttact
     6061 ttacaggtcg caagacaaaa agtggaaaca accgttcaca cgctatgaac tcaacaaaac
     6121 gtactgttaa acctaactta caaaaagttc gcgttatggt cgacggaaaa cctaaaaaag
     6181 tttgggtgtc aactcgtgct ttgaaatcag gaaaagttga acgcgtttaa ttgaatgata
     6241 cttggaccgt tcagatagtg aacggttttt tatttaataa aaatttttaa gggtgggact
     6301 tttgattcct accctttttt tgcgtttatt tttgttatac gaacaaatta tgaagttatt
     6361 tattctaaat tttatttttt tgtgaaaact agactctttt taagcagaga aaaacaagga
     6421 tatgataaac tgaatacaac ttcatagaaa tttggtggaa aataaatatg aaaaaaaatt
     6481 taaagtcatt ctttagagaa aacggacgct acgcagcagc tagtttcctg attccgtttt
     6541 tgattttagc acttgtttat ctaagtatcg gaatctatcc tggaagcagc agaagtattt
     6601 tggcaagtga tgccttttca cagttttcta attttcatgc cagcttccga aatgttcttc
     6661 ttggaaagca aagtattttt tatacatgga atgcttctct aggattgaat tatttatctt
     6721 taatttctta ctatctaggc gggatcttca cgccactggt gttctttttt ccaaaccaga
     6781 tgatgccaga tgcactgtat gttctgactt tgcttaaggt cggctgtgcc ggattggctt
     6841 tttggtttct tgcacgtacg ttcaagattc ctcgctgggg gcatgttgca cttagcgtca
     6901 gttacgcatt gatctcattt atcactgcac attcggagat catcatgtgg ctggatgcat
     6961 ttttttatct tccattagtg attttgggga ttcatcgtgt tatggatctt aaacggccga
     7021 aacttttatt tatcagttat ttactcttgt ttttatccag tttttatatg ggttttatga
     7081 ttggcgtatt ttctttctta tattttatcg cccgtctatt gacaaactgg tcagaatata
     7141 aaaaaacgat tgttccttat ggtatcactt ctttactggc aggcggcgct tcaatgatca
     7201 ttgtattgcc agcagtgctg gatcttcggt caaatggcga ggcactgagt cagatcacta
     7261 cgttgaaaac agaagcgact tcttatctgg atctaatcat gaaaaatatg attggtgttt
     7321 atgacacgac aaaatacgga tcgattccgt tcgtttacgc aggtctgcta ccattagtcc
     7381 tctgtctgtt ttattttgcc agtcggaaag taccgttgaa aaataaattt ttgtacggca
     7441 gtcttttcgc catacttatc gcaagttttt atcttgttcc attgaatttg ttctggcatg
     7501 gtatgcatgc accgaatatg tttttattcc gttatgccta tctgttctct ttcttagtgc
     7561 tcctgcttgc aggaaaagga tgggaaaaga tagaacaaga cgatcgtggg ctgttgttag
     7621 gaatcatttt ggtcgtagct gctctctttg cggtggcatg gggcatcaaa cctgaaggaa
     7681 gctacaccta tgtgacgatg acttcctttg tattgacagt ggttttctta gggatttatg
     7741 cttttgctgt gggattttat cagcataaaa aactttcatt caatcatctg agtatattgt
     7801 tattattgat catgtctgct gaagcactgg tcaatacgaa cagtatgatc catggcattc
     7861 ttgatgattg gaattatgct tcccgcagtt tatatactga tccttatcct gacttgaaag
     7921 ctcttgtaaa tgaaacaaaa aaagattccg actcgtttta ccgcttggaa aatctggatc
     7981 cagtatctgc aaacgattcg atcaactatg gttacagtgg tatcagtttg ttttcttcta
     8041 taagaaatcg taattcttcc tcttacctgg ataaattagg tttccgttca agagggacaa
     8101 atttgaatat ccgttataac aataatactt tgctgatgga tgggtttaca ggaatcaaat
     8161 ataacatttc taaaaacgaa agcacgttca gtaaatatgg cttccgccaa gctggtcaaa
     8221 gcggcgacta tacactgtat cagaatgtca atgcattgcc actagcattt actgcaccgt
     8281 tgtccgttaa tgatgttgag cagccagtga cagataattt gaccagtcag acaaacttga
     8341 tcaatacatt gtctggtcta aatgaacagt attttacatt ctatacacct actttgaaga
     8401 atcagttgaa tacgacgatc acaaagacaa cgactggcgt cacttactca gaaacagcag
     8461 ataaccagcc aaaagagctc acttgggaag tccatgtgcc tgcaaataca caagcttact
     8521 taagtttgtt cccaaccaat tttgcgcaat tagaaagttc tagtgcgacg atcactgtca
     8581 atggtacatc gcgaaaaacg caaatcaaca tttctggtca atactatgat cttggctatt
     8641 atcctcaaga gacaacagtg acatttactg caagttttta tgggacgaaa gaaataagtt
     8701 tcatggagcc taaagtcgtt ggtctagatg ttgtagccta tcaagccgca atgaatcaga
     8761 tcaaagaaaa aggtgtcgaa atggaagcca ctggcagaaa agcttctggg actgtaaata
     8821 ccgctgaaga ttccatgttg gtgacaacta ttccttatga caaaggatgg aaagctaaaa
     8881 ttgatgggaa accagtcaag gtcagcaatt tcaaagacgc attcttgatg ataaaagttc
     8941 cagcaggtaa acatactgtc actttcagtt acttgcctga aggatttacg ataggtgcaa
     9001 tcctttttgt cctatgtatt gcattgttta ttctttatgt ctggtacctt gaccgcaatc
     9061 gcccctttta tggcacgatt gatccagcaa tagaagaaca accacgaaac acacacagaa
     9121 gaagacgtag aaaataaaga aacgatggaa cctgttcaga ctgtccgcac caagggcaat
     9181 caaaacaacg ttccatcgtt ttttggttac agaaaaaata ggtaacaaaa agatgattca
     9241 cttatcaacg cctcgtccac aagtcacgat attcatcaaa gatgagaagc tgttttttga
     9301 aattgctctt tgtgactcag agttaaatga caggctcacg acctctttct taaacggtgt
     9361 tcaatcagct gaccttcggt attagctcaa caaacggcaa aatatgagga gctattttcc
     9421 cgtttgtttc tctaatactc aggtcaaaac gctgatttca cgacctcttc cttaaacggt
     9481 gttccagaag tagcttcttc gaaaataagc cgaaattttg caaaattttg agaagtaatt
     9541 ttcgtaaata ccttcttatt tcttgaagct gaccacttct atcataacct cttattcata
     9601 tttcacttca tacaaataaa gtccttcagg gtgtgctgta ggtcctgctg cattacgatc
     9661 ttttttcgca ataatatctg ggatactatc ttcaggcaat cggccattgc caattttcaa
     9721 aagcgttcct actaagatcc gtatcatctt atacaagaag ccatttcccc taaatgtaaa
     9781 caacaattcg tcttttgttt catttacctc catcttagcc tcgtagatcg tacgtatttt
     9841 atcctctatc gagcttcctg atgcacaaaa agaggtgaaa tcgtgagtcc ctaataaatc
     9901 aggaagcgcc cgctgaattt tttccaaatc aagtgggtaa ggaaaataac ttgcataatg
     9961 gcgtcgaaaa ggactgcgtg gtttccctat atccactcga aattgatacg tcttttcttt
    10021 gaccaaatat cttgcgtgga agtcttctga aacaatttct acttgtctta cagcaatatc
    10081 ctctggtgac tgggtatcta gcgcgaaacg cattcgttct aacggacgct cctctggata
    10141 atcaaaatgg attacttgcc cgatcgcatg tactccggca tctgtccttc ccgaaccaaa
    10201 gaccgtgatt tcttttccat ttgccatttt ttgtaatgtt ttttctactt cttcttgaac
    10261 agtacgccca tttggctgtt tttggaatcc
//
LOCUS       DACCCS010000083        10259 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.087, whole genome shotgun sequence.
ACCESSION   DACCCS010000083 DACCCS010000000
VERSION     DACCCS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.087"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..256
                     /locus_tag="IVK82_002460"
     CDS             <1..256
                     /locus_tag="IVK82_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAP6393494.1"
                     /translation="KQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKL
                     ATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSLEQLLSTV"
     gene            complement(297..1676)
                     /locus_tag="IVK82_002461"
     CDS             complement(297..1676)
                     /locus_tag="IVK82_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HAP6393495.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(1697..3244)
                     /locus_tag="IVK82_002462"
     CDS             complement(1697..3244)
                     /locus_tag="IVK82_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6393496.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3493..4272)
                     /locus_tag="IVK82_002463"
     CDS             complement(3493..4272)
                     /locus_tag="IVK82_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6393497.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4347..4805)
                     /locus_tag="IVK82_002464"
     CDS             complement(4347..4805)
                     /locus_tag="IVK82_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6393498.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(4817..6076)
                     /locus_tag="IVK82_002465"
     CDS             complement(4817..6076)
                     /locus_tag="IVK82_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HAP6393499.1"
                     /translation="MKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREARNYLIN
                     QMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVVTALET
                     MRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVKNRHGQ
                     TKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIPGIGRF
                     KARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIKPNSNQ
                     FVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARINYSNPT
                     NPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKGGITHH
                     PDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(6192..6506)
                     /locus_tag="IVK82_002466"
     CDS             complement(6192..6506)
                     /locus_tag="IVK82_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6393500.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(6652..8148)
                     /locus_tag="IVK82_002467"
     CDS             complement(6652..8148)
                     /locus_tag="IVK82_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAP6393501.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(8326..8805)
                     /locus_tag="IVK82_002468"
     CDS             complement(8326..8805)
                     /locus_tag="IVK82_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HAP6393502.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            complement(9181..9426)
                     /locus_tag="IVK82_002469"
     CDS             complement(9181..9426)
                     /locus_tag="IVK82_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6393503.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            9531..>10259
                     /locus_tag="IVK82_002470"
     CDS             9531..>10259
                     /locus_tag="IVK82_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393504.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHEL"
BASE COUNT         3183 a         2118 c         1611 g         3347 t
ORIGIN      
        1 gaaacaaacg attgaacgaa tatttgcgga tgcaaaagaa aagcatggga tgcgctggac
       61 caagtatcga ggcttggaaa aagtcgcgac gcatacgatg ctagtgtttg ctgcaatgaa
      121 tttaaagaaa ttagccacct ggctatggaa aggcaaggag cctttatttt tttgttcaaa
      181 aattcgaaac gaagtagata aaaagctgtt ccaagctcga gttacgagtt tggaacagct
      241 tttgtctaca gtctgaatca tttcttccgt tttaaagaag aaatgatttt catagatcaa
      301 cctttaacta aagctagatt cttttctaaa aagaatggtg tgtagtagcc cacaccgctg
      361 gacaacggta aaacttcatc cggcaactcg atgcccagtt cgttacggat gtactcttgg
      421 cgagaaatga ttcgcgacca aacatttgga tattctttgg ctagttgatc acgaagcgac
      481 tcatcagcta gcgcaatccc actctcacag cttgcgccgg catagccagc aactgatgga
      541 ataatatcaa tttgtagtaa cattccggac ttaagagtaa tttctgagtc ttttttcatt
      601 ggcgaagaca tccactcttc atcagccgta agatgccccg gatttaagtg ccatttgtat
      661 tccgatttag gcaagacttt gcctattact gaatatagct ctttaccttg cattcctatt
      721 ttgatatttt ccaaccatgc gaccacggcg tcgtagtatg gagctgcaac tttttcaatg
      781 taatcctttt gcccttctgg taggtcatct gtcgttgcaa ctgcatagcc acttcgactt
      841 gctaatccgc ctttaaagcc cgtagtaatt gacattttat cgcctagttt aacttgttta
      901 tcagttggat atatataggc gttctcaaac cgttccccag ttgcagcaat tggaatgaca
      961 ttatttggtt ggccgtactt aactaagtgg ttaccaattt ccatttctgt ttttcctacc
     1021 tctacttcat caatagcgtt catcacacaa tctgaagcca aggatgagcc aaattcataa
     1081 tatgccactt cattagcagt atttaatgag cgtactccgt aatcactgtg aatgaacagg
     1141 tcggtgcgat tgctaacggc atcttcccct actaattcta caatcgaact tacaatgaat
     1201 gccggcaaat cttggatttg gtagttatcc tcttctgaag taaacatctt ccaaccaaca
     1261 agcccaactt tcttaccttt actgatttta gcattggtca atacttcagt tagtggtttc
     1321 tcgttatcca tcggttgatc cggtaatgag aagagtggcg tgtggattaa ttctgcctca
     1381 attcttgaat agtccaccat tttagtattt tcatttccta gaactaagaa agcttcaccc
     1441 gtctgatgta ataccaacaa gctttcttca aagcgagtaa caaaacctgt taaataagag
     1501 aagttcccgc catgttccaa atcacaatag attacgaggg tatctatctc tgacttttcc
     1561 atcgctgcaa gtaccttttc tttacggaac ttcattgttt catcagtcaa ttgatatatt
     1621 ttagcttcat tatctttttt aggtgggtta acttttttta gagttacctt agtcatattg
     1681 tcactacttt ctgtttttat ttacgcttca tttcattttt cagagatcga gttgcgtttt
     1741 ccacttgtcc tccatatacc acttgtaaat tattttcgtt aattttaacg attcccattg
     1801 agccggtctt tttcaagata tgttcatcaa ttgttgctgt atctttaaga actagtctca
     1861 gccgagagat acagttatcg atttcttgaa tattaccagc accaccaagt ccttgcaaga
     1921 ttagtttgcc aagttcgttt tctgtcattt cttcgtcctg atcttccaac acatcatcgt
     1981 tggatcgacc aggagttttc acatcaaatc gctcaatgac atacttaaat acaaagtaat
     2041 atgcaactgc ataaacaagg cccaatgcta caactaagta ccaacgagta taagtacctt
     2101 gtaaaattcc gaagatagcc aaatcaataa atccaccacc ggcattccca actgcgactc
     2161 cgaatagatt cattaacata aaggataaac cagccatgaa tgcatggaag acccatagaa
     2221 tcggtgagat aaagataaag gcgaattcaa ttggctcagt aatcccggtt acaaaacaag
     2281 tagccgctgc tgcaatcaac atccctttaa tctttggacg ttgcgccaaa tatgaagttt
     2341 tgtacattgc atagcatgct gccggcaatc cgaagaccat aaatggatga gtaccttgtg
     2401 tcaagaagcg agtagcactt ctcaaaatat ccatgtcttt tactggcaat gctaattcag
     2461 tattgaagat ggttaatgct ccagaaactg tttcaccatt gatagttgcc gtaccgccga
     2521 ttggtgtaaa tcgaatcaac tggtttagta catggtgaag acctgttgga atcagcaggc
     2581 gctctgagaa gccatataaa aaagttccaa ttacgcctga cttaccaatc aaaccgccta
     2641 aagcattgat agctgtattg acaaaaggcc agatgaaaaa tgaagcgatt gagatgattg
     2701 gaatagttag gatgatcata attggaacaa aacgattgcc actaaagaaa ctgaaagctg
     2761 ctggtaattg ttttgtgtga tagcgattgt ggatcatggc tgtccataat cctgcaatga
     2821 taccgccgaa aacacccata cgatatgtga atgtgccaag taaagtttca tacatattgg
     2881 aagtttctac tgctttgatt tgactcattc cgtcagacat caatgtttca acagaagtgc
     2941 tttccgctgt gatgcctcta agctgaagca gataattgat aactacatgg aacagaataa
     3001 atgcaataac cactgaaaat gctgcaattt ccttatcttc tctagacatc ccgattgcta
     3061 ctgaaatagc aaatagaata ggaagtagtc caaacatcgt accaacaata gcctgtactc
     3121 caccaacaaa tccttggatc acggttgcat tagcgaagct ttctcccacc aaagtaggat
     3181 tttgtaaaat tgaaacgatt cctagtagta gtcccgatgc gacaataata gacaacggac
     3241 ccattaatga tttccctaac ttctggatat aagttctcaa tataaacacc ttcttctttt
     3301 tagttgatat aaacgtttac atcccttgta caacgaaagt atagcaccct gaaaaagcgc
     3361 taacaatacg ataggtctga taaaaatcaa cattcagcac ttggttgttc attcaaaacg
     3421 tgaatcttca atcacagaaa aatacgcgct atcaagcacg tattttaggt gtaaatatat
     3481 gcatcaactt tattaatcaa cgctactttg tttgtaatag ttaatatacc cttcatacag
     3541 taagctaaga gcaagattca aggtgcagaa tgatgagttg ttcaactcat tcacatcatt
     3601 aatgttggtc acttgatagt ataggctata gtctgacaga ctggctaagt tattttgacc
     3661 aaatcgtgta actgagacca actgtgtccc tttcatatgg attcttctta aagtatcatc
     3721 aatccgatca acacgtccgg acaatgatat gacaaatagt aaatcattcg aggtcaaagc
     3781 ttttgaaatc aggtctaatt cattcttttc cggtacaacg ataatgtaga ttcccaagtt
     3841 ccacatgcag cgtgcaaact cattgagcat cattctttgt ccatatcctg ttgcgtaggc
     3901 aaatatctta tctgcctttc taatcgtttc atatatatcc tcaacacttt tatcatcttc
     3961 aaacaatttg gttgtcgctt gaacatctcc gagtaatact tccggtaaaa acaaactact
     4021 tttatcgact ctggtttttt gcatgcgtgc aaattcctct ttaaggtaat atcgaaattc
     4081 gccatatcct ttgaatccga gtttcttagt tagtctaata atcgttgagt cagacacatt
     4141 tgcttgtttt gctaattccg atacagtttg actgatgctg atttccatat tttgagtgat
     4201 aaaacttaat acataatcat cattctcatt caatttactg tgatgttcat ttactaattg
     4261 ctccaatgtc ataatcagac ctccaaaaat aataaaaaag attgctctct cattttaata
     4321 cgaaagcaca atctttttaa gtactattaa attttgaaaa gaagttctcc ttcagaaacc
     4381 tttttttcag agacttctag tttaataccc ttatcttcgg gaaatagaac cataacaaca
     4441 tctgacagtt tttccgcctt gatttggtca caatcaaaaa tcaccaatga ctcgccggat
     4501 gatactcgtt gaccttcttc aacgagcact tgaaacccga ctccctttaa ctgaacagta
     4561 tcaattccaa tatgaatcaa taaatctcta ccttgattac tagtcaatgt gagtgcatgc
     4621 tttgttggaa atatcgattt aatgacaccg tcaatcggtg ctaccacctt agcgtttgtt
     4681 ggttcaacag cgaatccttc gcccatcatc ccttgcgaaa atacaggatc gcttacttgg
     4741 ctaaggggaa tcagatttcc tgatactgga gcaactaatt tttcttttcg actaaaaaat
     4801 cccattgact tactccttaa tcttttgaaa tgtccatcac aaggtttaaa agaacatccg
     4861 cccctttttt gacatcctca aaatcagtcc actcatcagg atgatgtgta atgccacctt
     4921 tggatggaat ataaatcata tttgttggga caaagtcggt catcatcatc gcatcatgtc
     4981 cagtaccatt gttaatgact tgatagctat agcccaactg atcgcaaata tgtttcatct
     5041 tctcaacatt accttgatcc ataacacttg gatttgtagg attactgtag ttaattcgag
     5101 ccatttcctc cagctcagtt ctaactccag tttccccttg aatcactgat aactcattca
     5161 acaacttttc tttaatctct tgaggattag ttgtctttgt atcaaaggtt ctgatttcga
     5221 ttaaagcttc gacaaagtca gggacaaatt gatttgagtt cggtttgatt gtaagtttac
     5281 cgacggttcc ggcaatccct tcaccaagat tcttcatcca ctgttcaaca aaaagaacaa
     5341 atttagaagc agcaactaaa gcatctttcc gtttattcat tggagcagtc gagtcctcag
     5401 tttctccata aaaacgtgct ttaaaacggc cgatgccagg aataaattca atcaatccga
     5461 tatctttgcc ttcatcttcc aggactggtc cttgttcgat atgaagttca ataaaatttt
     5521 tgattgtttt tggatctcgc ttagcggcct ctaaatctgg ttccactcca taagcacgaa
     5581 gagcttccaa ttttgtttgt ccatggcgat tttttactgt ttccaactca ctcatggtta
     5641 atgttcccat catagctcgt gaatttgaga cacccgtact tggcccaaat gtctcgccct
     5701 cctctgcatt catcagaatt aggtcaattg ggtaatcatt ttcaaaatta ttttcttcca
     5761 gaatccgcat tgtttccagt gcagtaacaa cgccagccac tccgtcaaat ctgcctccat
     5821 taacaactga atcataatga gagccaatca tcactgatgg tgcatcagcc tttttaccgg
     5881 ctctacgacc aaataatgta gagaagccgt cttcccaaat ctctaaaccc agttgttcca
     5941 tttgattgat caaataattt cttgcttcac gatcttcttt agagtaagca gagcgggtca
     6001 ctccttcacc tggagttgca ttgtactcgg ctaaattttc gatatccttt tttatacgct
     6061 caaaatttgt cttcataatt aaatcctcca tttcctacaa gtgaatgtta gcacccatag
     6121 aaagcggcta caatataatc aatgaagatt gaatcgatat tcgaaaactg aatattgctt
     6181 cggttaatga cttagaaatc attatcgtag attccaccat aagaactttt tctcgtctca
     6241 taacacaact cgagagtgga ttggtttaaa aaactctcga taaatgcgtc cactttattg
     6301 acacagatcc atggatcgat cgcaccttga aatttaaacc cagaaatagt cccatcatct
     6361 tctataccga ttaccagctt acctccatcg gcatttgcaa acgctactat gtgttttata
     6421 atatctctag gcttaatccg tgcacttttt ctatcaaaat actgatcttc tttttcactt
     6481 ataaactttt gaatctcata atccatttaa aatttccctt cttaaatttt tttattatag
     6541 agccgtatat tcaatttcca caaaaaaata atctaaaaca aaacagacac cttcattttg
     6601 atatgttctc ccacatatga atcggtcgct atacagcatt acatatagta attattcata
     6661 ctgcgaaata aatagctcca aatctttagc tggcgtagtt aagcttaaat tcgaaatgaa
     6721 agtttcattg actattccac tttcatcaaa tcccttcttc aaccaatcag agttccacaa
     6781 cgtaaatcct ttaggattag aaactttctt acataatttt gcactatagg atttctctct
     6841 tgaatgaatt aaagaaagtc tttgtaacca aatttcaact atatccgtat taggagtatt
     6901 aacaaatttt cttaagattt tatccaagta taagttgatt tcttctacag caataaactc
     6961 aaaaagctta cctaaaatca caatacaagc ctccaaagtt ctaggattct tttgcattat
     7021 attaacaaga atactaatca attggtagat atcatctggt cttttgttta aagtagaaac
     7081 tctatgtgta tagacttcac ttagcgcttt acttaaactc ccgcaatttg gatatttgtc
     7141 tcctaaaagc tttacctgta gcaaatgctt ttgaaggcta agcttaaact tccacttatt
     7201 attacttttc tctgtaaaac ttgcatgttt aagatcccag taaattttat caggcttaat
     7261 tgcatctaaa attatattat ccgataagaa tgttttattt gaatttagct tcaaatttag
     7321 atccgataat acttcagata acaatctgat aatcacttca gcttgatctt ttcgaaccga
     7381 aaaaattctg taatcatctc tgtaacgaag tattttgaac tctttaatct ctgttttttc
     7441 tatcttgttc agtaacattt tatcagcata cccaagtact atttcagcta caaaatccat
     7501 caatacattt ccttgaggta taccgttcgt ttgtccattt tgtaaatatc gtattttaga
     7561 atctattaag tttccaacgc cttgagtatc ttttctatgt gtcttagccc attcttttga
     7621 atgtactgcc cacgggatag tatgtgtgta cattgagcta taacaatcgg taatatctga
     7681 atgaatgcag tagtcatact ccaaagcata actaatctgt gcttgttcaa ggttctccca
     7741 ccaatttaaa atagtttccg cagtatcgct ctttgaagaa atagattcca caggaagtga
     7801 tatacattca attcgcttgt cggcttggaa ttcttcaaac ttatttttga tgtgattcca
     7861 attatttttc tgtgtaatcc cgtttactaa gtctacatat actaatggat gtaatatttg
     7921 aaggggtctc caagcataat taccatcttt attagctaat attcgatgat taacatcaac
     7981 tgcctcagag agcgatctct tatgtctaac tagttcagta aatggtttgt tttttaataa
     8041 atccactgct ttctcaatgc ctactgataa atcaatatag ttgggcaacg atgtactaaa
     8101 aaaacttgat gtttttaaaa agaattgctt tgcttcttga tgattcatct ctaaaatact
     8161 ttttctagtc ctagtcaaat tcaaaatctc ctagatcaat taagttgtaa tgtaagagga
     8221 taccctaagg ttacacaccc tatttactat tataccagta ttcattcgtg atacataagg
     8281 gtgtaacatt aagaactcta tctaaaagac tacaagtagt cacctttaat tgtgagtaat
     8341 tttctttatc caatccttgt gcaaccttgt cgtcacccct tgtatatccc cgtaccccgg
     8401 atgtttcgga atatcaaaaa tgtgctcaaa ttcctgattt tcatcagtct cccactcatg
     8461 attcaacggt tcatgccact ctataaacct tgaccataaa atgttctttc tataaaactc
     8521 aataggaatt tcaaaacagt aaaaattgta tttgctaaaa tctgtaaaat tatctttacg
     8581 agcatacttt aaatggcagg tatctcgcca caaccaaatt aaatctcgat ccaatggtaa
     8641 ctgaattcgt ttcgtatact cttcttcaag ccaatgaagt gcaccaactg cattcggatt
     8701 atcgaaaaag acagataatt cagtatagcg tttctttgga taaataaatc cttctgaatc
     8761 aacagattgc aaaaaatcta ctggttgaac cgtgtacact ttcatttatt cgccaccttt
     8821 ttttgactac ttatagtcat ttaccaattt gaaatctgca tactttttca ttcaattgag
     8881 cccagcatta attgtacacg agttttcttc actaacggat agtatcagga tatactaata
     8941 ctatcctgaa tagcgaaatg aggtagccaa aatgagaaaa gtcgatgaat atatcaaaaa
     9001 gaaatcccaa aaaagtaaag tatggcaaag aacatttgag ttagagtata agaaactctc
     9061 agatagcctc aaaaaggaca cttataatcc aaatgaacaa agtaaagcag ataaccaata
     9121 aagattaccc tcccatattt aacattgctt cacgaatcag taatcaatga ctacaagtag
     9181 tcattgaagc aaatcttcat aagatacctg tagaatatcc ctaatagctc tcaattgatc
     9241 cagcttaata tgttgccggc caccttcaat cttaaccaat gtttctcttg tcatagcgat
     9301 tttactgagc tggagttctc gcaccagttc tgtctgtccc attccctttt ctaatcggat
     9361 tcgtctgata ttgctaccga ttacattttc tttatctatt aacttttctt ccttcaaaaa
     9421 tagcacgcct tttggactaa aattagtcct ttttcttatt aatcttaaag atttggtatg
     9481 ctacaatggg actagtatta gtccatattc aattttaagg aggaattttt ttgaagaaat
     9541 ttgcaatcat tctaacctgt gctttactca cgggagctat aagtgggtgc gccacttctg
     9601 acacaaattc tacaagtact gaaacaagta caacaacctc agaaaagaat tatcctatac
     9661 atttttttcc agttccagat ggatttgaag cttattatca tgataaaaaa ctagatgttc
     9721 cattgtcaga aagtaactct attgaaaaag ttttgccaat tgaaattgaa aaaaatcaag
     9781 agaatataaa actcaaggcc gagcttccca tactgaacag cacagaaatt gattggcacg
     9841 agaatattaa agtaactatt gaaaaaggag cgttagcaca actttatact gaaaaagttg
     9901 aacaattttt tgagtctggt gccaataaag acttttcaaa aataagcgat tattcggata
     9961 acttttttaa tgagacccaa gatgaaatca cgaactcaac ttttatggct gaagatatgt
    10021 acaatatgac gttaaaaaaa ataaaagctt acgaccatcc attaaatctg acactaaata
    10081 atggattact ctcttttgta ttagcaggtt acgttgacta tgaatattca ccagattatt
    10141 tggagacacc tcttcaacac gagaacaatg atattaattt tagatttgtc tacgagtcaa
    10201 aaagtaaaac atggttagtt gatcaattaa gcactataaa cacaagcttc catgaatta
//
LOCUS       DACCCS010000084        10215 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.088, whole genome shotgun sequence.
ACCESSION   DACCCS010000084 DACCCS010000000
VERSION     DACCCS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.088"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            248..3475
                     /gene="fss3"
                     /locus_tag="IVK82_002471"
     CDS             248..3475
                     /gene="fss3"
                     /locus_tag="IVK82_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP6393505.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3588..3902
                     /locus_tag="IVK82_002472"
     CDS             3588..3902
                     /locus_tag="IVK82_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6393506.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3915..4289
                     /locus_tag="IVK82_002473"
     CDS             3915..4289
                     /locus_tag="IVK82_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6393507.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4299..4634
                     /locus_tag="IVK82_002474"
     CDS             4299..4634
                     /locus_tag="IVK82_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP6393508.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4717..5862
                     /locus_tag="IVK82_002475"
     CDS             4717..5862
                     /locus_tag="IVK82_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP6393509.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQCATCF"
     gene            6577..8436
                     /gene="ltrA"
                     /locus_tag="IVK82_002476"
     CDS             6577..8436
                     /gene="ltrA"
                     /locus_tag="IVK82_002476"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6393510.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
     gene            8557..8814
                     /locus_tag="IVK82_002477"
                     /pseudo
     CDS             8557..8814
                     /locus_tag="IVK82_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8817..8970
                     /locus_tag="IVK82_002478"
                     /pseudo
     CDS             8817..8970
                     /locus_tag="IVK82_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            8993..10198
                     /locus_tag="IVK82_002479"
     CDS             8993..10198
                     /locus_tag="IVK82_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6393511.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         3541 a         1744 c         2172 g         2758 t
ORIGIN      
        1 accttgattt ataaaggttt tcccgtaaat atattttatt atatttgatt aaatttgaaa
       61 tgtttgtgtt ctaaaatgtg ttctaattta aaggcagtat atttcgtatt ttagattaaa
      121 tttttaggct cattcattct caaatccatt ctattcagtc aataaaaatt ttaaaaagac
      181 tttataaaca aagatgttca agcgagcatc ttattttttt gtgcttaatt tgaaaggaga
      241 acatttcatg tataaaaaac aaaaaaagaa attatgtgta ttaactattt tgagtttggc
      301 tgttcaagca attgttggag caattgctcc tacgattact tttgctgatg aaattaccca
      361 tccacaaacg gtaaccgtag aactagattt agcacatcaa tatgctgttg aaggaacttt
      421 tagtgatggt cgtccaatgt cagaagtcac tgttccgcat tatgcagtgt acaacggctt
      481 taaacaagat attttctgta ttgaaccagg agtcccgatt tacaatgagt tcactcctgg
      541 ctatgagaaa aacccgttac cagatatgtc cgataaagcc aaattagttt ccgttttatg
      601 gaaaaaagta ggtacagatg cagatactca tattgtggca cagaagatga tctggcaaga
      661 agttaatggg tatacccttc actcaataaa acgatcagat ggtagtgccg taaatatcgc
      721 agccattgag gcaaaaataa atcaagccat tgacgattat cagaagaaac caaccttcca
      781 taatagtacg gctaaaacag tattagggca atcgactact gtgacggata cgaataatct
      841 gaatttgtca gagttcgatg aggttgtgga aaatgccgca aacattgatt atcgtgtgaa
      901 tggtaatcag ttgataatca ccccaaatgc caattcaaaa gaaagtggtg tgctgacgct
      961 taagaaatct gctggtaccg gaacacctgt cgcctataaa aaggctggcc aacagacttt
     1021 aatggccggg gcgattgata aacctaatac ctacacaatc aaaattgatg tggaaacgga
     1081 gggttcttta aagatcaaaa aagtcgataa agaatcgggt gatgttgtac caggaacggt
     1141 tttccattta gactttggaa aaactttacc tgcaaaagac gtgactactg acaaagaagg
     1201 cattgctaca ttggatgaga ttcctcatgg tacaaaagta accattaccg aaaaatcagt
     1261 accggcgcct tatacgattg ataccacccc catgactacc accattaaag caggcgaaac
     1321 catttacgtg acttcaaaaa acgcacggga aaaagggcaa atcattctgg ataaaagcgg
     1381 agttgaaaca gggagtgatc tttggaatga caattattcc ttagctggta atacgtttgc
     1441 cattcgcaaa gacagtccaa ccggtgaaat cgttcaagaa atgaccacgg atgaaaacgg
     1501 tcatgcggaa acaccaaaag agattgctaa tgcgttggaa ttgggaacct attatgtgac
     1561 cgaaactaaa gctagtcatg ggttcgtgaa tacgttcaaa cctgtaaaag tcgaattgaa
     1621 gtatgccaat caaaccgtag cccttgttac cagtaacgta aaagggcaaa atcaagaagt
     1681 cactggcgaa accactttga cgaaagaaga caaagatact ggggataaag cacaaggtaa
     1741 ggcagtcttt gagggtgctg aatacacctt gtttactgct aaggatggaa aagctgttaa
     1801 atggagtgag gcttttaaac cagagttagc gaagggaacg aaaggttctg atgaaacggt
     1861 cactttgact ttagacgaaa agaaccaagt tgccgttaaa catttagcta tcaacgagta
     1921 ttattggcaa gaaacaaaag cgcccgaagg atatagcctt gatgaaacaa agtatcctgt
     1981 atccatcaaa aaggtggatg atagcgaaaa aaatgccgtg attacccgaa atgttacggc
     2041 aaaagaacaa gtgattcgct ttggctttga tttctttaaa tttgctggat cagctgcggg
     2101 cactaccgaa actggcttca atgatctgac ctttaaagtg tcgccattgg aagggaccaa
     2161 tgaaatcaca ggtgccgaag atgaagcgac cacagcttat aacgagcaat tagggtttga
     2221 tggttatggc aagtttgaaa atcttcctta tggggattat ttacttgaag aagtagaagc
     2281 cccagaaggt tttcaaaaga ttacgccatt agaaatccgt tctactttta aggaaaacaa
     2341 agaagacttt gtgaagagtg agtatgtctt taccattacc gaacaagacc aaaaacaacc
     2401 aattaaaacg gtgaccgttc cttacgagaa gctgacgaac aaagcatttt ctgttagttt
     2461 gaaccgttta atgctctatg atttgcctga ggaagaagat agtttaactt ctcttgcgac
     2521 ttggaaagac ggaaacaaag aattaacttc tctggattct accgagctag ttgataaatt
     2581 gagttacaat ctacatgaaa tcaaagaaga ctggtatgtg gtagcgcaag ccattgatgt
     2641 ggatgcgaca aaagctgccc aagaaaaaga tgaaaaagcc aaaccagtgg tgattgcaga
     2701 aacaagcgca acgttggcga acaaagagaa aacaggaact tggaaaattc agcataaatt
     2761 aaccgccgaa caagttttaa ataaaactat tgtcttattc aactatgtgt atgaaaataa
     2821 ggaagctttt gaagcaggcg ataagccagt agcgaaggat gttagcttga ataaccaagc
     2881 ccaaactgtc agttgtacag tggaacacca tgtttctatc caaacaaagg cccacctaga
     2941 aaacggttct cagaccttta ctcatggtga tgtggtggat atgtttgatg atgtgtcgat
     3001 tactcatgat gtactggatg gctcaaaaga agctttcgaa acaattctgt atgctttatt
     3061 accggatggt acaaacaaag aaatttggaa atctggcaag attgattatg aagtaaatga
     3121 taaagaattc accaaaactg tgcttgcgaa aaaagtagat accggaaaat atccagaagg
     3181 aaccaagttt acttttgcgg aaatcaatta cgataaagat gggaatatca atggaaaaca
     3241 caatgaagat ttgaaagaaa aatctcaaac cttaacacca aaagaagttc caaccatact
     3301 aagtacgccg aaacaaccgg aaacaccaac tgttccaagt ggctctcaag aatctagtcc
     3361 cacagtgaag accttccctc aaaccgggga gaaaaattcc aatgttttac tgttcattgg
     3421 ttttactttg atttttgcga cggcgggcta ttatttctgg aatcgccgga actaaggtga
     3481 tgagatgata caagtaaatc ctaagaacca acgtgcgcga ccgcctccta aaaaagtagt
     3541 cgcgctattt ttgtatccaa aactaacaat gaaaagagga aaagaagatg gaattgaaat
     3601 ttgtcgtgcc agatatggct gaaacctttg gaaagttaag ttatgccggt gaaggagaaa
     3661 ttataacaga aggatatgga cggaatacta cggtgattgg tcgcagttat catttgtatt
     3721 ccagtaagca acgggccgat gatattgagg tggtagtagc tgcggaagcc ggtgaaaagg
     3781 attttgatca agaccaaccg ctaaaagccg tgaatcccca tttggttgcc aaaggctatg
     3841 agattgagaa tcgtgggttt actgattacg tgttgtatgt tgatgattta gtgaaagcat
     3901 aggaggaaaa gaagatgaga ttaacagaag gtattgtcgt agattcaggt ttaacatttg
     3961 ggaagttgcg tttttccgca ttacgccgag aagtacggaa gcaaaatgag gatggaacaa
     4021 ttggtaatga ggtaaaagaa cgaacctata atttaaaatc ctctgcacaa gggcgaatga
     4081 ttcaagttag tatcccagct aatgtaccct tacgcgagtt tgcttatgat gcagaggtag
     4141 agttggtcaa tccaatcgtg gataccgttg ccaattatgt ttttcgagaa ggaactaccg
     4201 ttaattggtt cattaaggcg gatgatttag tcttgaagcg gcagccgaat caaggaaatc
     4261 ctacgaacca aaatgaaaga aagaaatagg tgaagattat ggaaattaat gttgaacata
     4321 ttttagattg tctcgatcaa tatggaaaag gcgagctgac tgaggaacaa ctcgttaaag
     4381 cattaacctc tgatgaaaaa atgtttctga ttatacatca aggtttactt ggagaaaata
     4441 gcgacagtga agaagactct gatgtactag actggttagg agaagaagag tctttcttta
     4501 tagttgtcga agtaaatgaa aacttatgtc ggcaggcaga atccattttg gaagaaattg
     4561 gtgtggagat gccggatgct attgaaggct ttttaaatca attggttgaa acgaaacaac
     4621 tcccctcctc gtagcagtaa atgactagca taactttttg aaattgccta atgaaatcct
     4681 attgggcttt ttcgattgga ggtggaaacc atcaaaatgt acaaaggaca tcgcatacgc
     4741 gcaggagatc agcacttggt ttatcacttt gttcttggct ggctactcgc tctgttcatc
     4801 ggttggatga gcgtctttta ttttcaagag tttagacaat ttgatatttc taagctatca
     4861 ctttctacta ttgaaatcgt ttggtctata aaagatttag tatgtctgct tggaagtctc
     4921 gctttttcag gagctatgat attgctttat attcactttt ttcttgatca ttggcgaagc
     4981 ttgtggcatc gacaaaagct agctcggatg attctagaga accattggta tgaagtgaaa
     5041 caaactcaaa gtgaaggctt tttcaaagat ttgaatagta gccgaaccag agagactatc
     5101 agttacttcc ccaaaatcta ttatcgaatg aaagatggat tactgtctat tcgcgttcaa
     5161 atttcacttg ggaaatacca agatcagctt ttaaaattgg aaaagaagtt agaaagcgga
     5221 ctgtattgtg agttagtaga aaaggaactc aaagactcct atgtggagta tactttactg
     5281 tatgatatga tcgccaatcg aattgggata gacgaagtag tggcagaaaa tgggactctg
     5341 cgattgatga aaaatcaagt atgggcttat gattctttac cccatatgtt aatcgctggt
     5401 ggcacgggtg gagggaagac ctatttcctc ctcaccatta tcgaagcact attgaagtca
     5461 gatgcagaat tgtttatcct cgatcccaaa aatgcagatt tagctgattt aggtacggtg
     5521 atgcctcatg tttattcaca aaaggaagaa atttctgctt gtgtggaaga tttctatgaa
     5581 cgtatgatgg ctcgtagcaa ggcaatgaaa gaaatgtcta actacaaaac aggagagaat
     5641 tatgcctatc ttggacttcc acccaacttt ttaatctttg atgaatacgt ggcttatatg
     5701 gagatgttaa cgacgaaaga aagtgcggtg attttgaata agctcaaaca aatcgtgatg
     5761 ttgggtcgtc aatctggttt ctttctgatt ttggcttgcc aaaggccgga tgcgaaatat
     5821 ttaggagacg gaatccgcga tcaatgtgcg acatgtttct aactgaaaag gttaggcact
     5881 taaaagaaga aaacataagt ttccttttaa ggagaactga tattttgagc agtggaatga
     5941 tggggtaacg ccctgaaacg ctcccttaat actccgactg gctgtttaac atatagctgt
     6001 tagatggtca gaagctcggt gaagtcggct gaaagttacc gtaaatatga taaattcata
     6061 tacgaaagtt gttcagaggt gaatgatgta acctataggt cggaggtcta taaaatactc
     6121 acggtgagaa tgtaagtaat actactgacg aacttgcgaa tgaaagaatc tatacacgga
     6181 ctgtatagaa atgtacagga ttctaccgta tcaacggaac agaatgtatg ttgtggttta
     6241 gtaagattat tggttatgaa caaccatgca atgttacagg cattggaaat atacaggttc
     6301 aaagcaagca cctaaaagta tatataaaga taaaaatatc gaaacatggg aagctggcaa
     6361 tgtggaggaa actatattcc attgaaacat ttgtgacgaa gaataataag cacatttatg
     6421 ccagtgaaag tggtggcaca gtaccaatga ggatatagaa atatatcgga ggaatagcca
     6481 ctagtcaaaa gaaatcgtta attaaatgat gtatttcata gaatcgagta agacaaaata
     6541 aacactacaa cctaagtaaa caaaggtagt ggtgctatga ataaagtaaa aacaaataaa
     6601 acacttcgtc atagtgaata ctatggcatg acacaaatat ttgatgaact gtatgtaaaa
     6661 agtgataaaa aagaagtttt tacagagctc atgacaatta ttatgagtga tgaaaatatt
     6721 cgactagcat acagaaatat caagaataac aagggcagtt atacagcgag taccgataag
     6781 aaaagtattc gagacattaa aacactgtca gaagatacct ttctaactga ggttaaagtt
     6841 ctttttcgct gttacaaacc acgagcagtt agacgtgtag aaattcccaa accaaatgga
     6901 aaaacacgcc ctctgggcat tccatcaatc tgggataggt taatacaaca gtgtatttta
     6961 caagtcttag aaccgatatg cgaagctaaa tttcacaatt atagttatgg atttcgacca
     7021 aaccgttcca cacatcatgc gattgctacc tgcaatcgac tggcaaatag ggggaagcta
     7081 cactttgtag tagatgttga tattaaagga ttctttgata atgtcaatca ctctaaactt
     7141 ttgcgtcaaa tgtggacgat tggaatccgt gataaatggc tattaggtat tattcgatcc
     7201 atgctaaatg ctcctatagt cctaccaacc ggtaaagtcg aacaccctaa aaaaggtgta
     7261 cctcaaggtg gtgtcctatc ccctttacta tctaatatca tattgaatga attagattgg
     7321 tgggttgctt cacaatggca aaataccacg attgagacag aaaagattat ggataggcga
     7381 gataaaggca aaggcattga taagactaat cagtatcggg cattaagacg aacaaactta
     7441 aaagaaatat acattgttcg gtatgctgac gacttcaaaa tcttttgtag aaaacgaagt
     7501 catgcagata aaatatttca cgcaacgaaa atgtggctta aagaacggtt atcacttgaa
     7561 attagcgaag aaaaatccaa aattgtcaat ctcaaaaaag gtaaatcaga atatctagga
     7621 tttgaattaa agctaatcaa gaaaggtaaa aaatacgtag tcgaaagtca tatgagcaaa
     7681 aaatcaatga caagagttaa aatccaatta aagaaacaat tacgtaaagt tgccagaccg
     7741 aagaatcatt gcgaacaagc aaaagaaatt ggattatata attctatggt gattggaatc
     7801 catcaatatt atggaattgc aacctgtgta aatctcgatt gcagtaaaat agcctataca
     7861 ctaaaaccgt tcatcaatca caaactgccg acaaagaaac atggaaagat attaaataca
     7921 tttatcaaat cgaagtatgg aaaaagtaaa gaaatgcgat ggttgaataa tgtgccaatt
     7981 gtaccgctca actatgttca gtttagactt gctgtccccc tatcagaggg aacttgcaag
     8041 tacactgaaa gtggacgaag caagatacac agtaaattaa aattagacct agccctttta
     8101 cttcacatga tgagaaatag taattatgaa agaagtattg aatttgtgga taacagaata
     8161 tctaaatatt ctgctcaaaa gggaaaatgt gctatcacgg gtaaatttct cgaatacgaa
     8221 gaaatccatt gccaccatat cgtaccagta aaacaacagg gtacagacaa atacagcaac
     8281 ctcattatta ttcacaaaaa tgttcacgca ttagtccatg ctatcgaaga aaaaatcata
     8341 cacaaatacc tgaatgtatt aaatctaacc aatgaacaaa tagaaaaact aaatgaatta
     8401 cgtgtcaaag ctggtaatag tgttttaaca gtttaacttg aaaaatatat catacttaac
     8461 attcttttga tggaacgccg tatgaagtga aagtttcatg tacggtgtga agcgggggaa
     8521 aatctggaga tgacatcaaa ggattaccta tcgctattta atttcagagt ggctttaggt
     8581 cgtatgtctg aaatgggcta tggcatgatg tttggcagtg acgtacaaaa ggatttcttc
     8641 ttaaagcgaa tcaaaggtcg tggctatgtt gatgtaggaa caagtgtcat atcagagttt
     8701 tatactcccc ttgtaccaaa aggatatgat ttcttggagg aaattaaaaa gttatccaac
     8761 agcagacagt ccacgcaggc gacgtgcgaa gcggaagtcg caggtgtgga ctgatcttgc
     8821 tggctggtgt ggcaatagcc acgccagcac ttaacccctc ggtatctaac aggggggtac
     8881 aaatcgacag gaaacagtca aaaaaacatt agaaaatcct ttggttacaa gggatttaca
     8941 aaatttcagc gtatgtcaaa tgggctttaa aagttgacat acgccttttt gattggaggg
     9001 atttttactg aatgaacaaa cttggttaca gcatttaaaa gaaaaacgct tggcttatgg
     9061 actatctcaa aaccgtttag ctgttgcgac tggtattaca aggcagtatc taagcgatat
     9121 tgaaacagga aaagtcaagc catcagagga tttacagcag tccctttggg aagctctgga
     9181 acgcttcaat cccgacgctc cccttgaaat gctgtttgat tatgtaagaa ttcgctttcc
     9241 gacaacagac gtacagcagg tggtcgaaaa catcttacaa ctgaaactgt cctattttct
     9301 tcatgaggac tatggtttct attcttattc agagcattat gctttaggcg acatattcgt
     9361 cctttgctcc catgaactgg acaaaggagt tctggtggaa ttgaaaggtc gtgggtgcag
     9421 acaatttgaa agctatcttc tggcacaaca aagaagctgg tatgagttct ttatggacgt
     9481 tttggtggct ggcggtgtga tgaaacgcct tgaccttgcc attaacgata agacagggat
     9541 tttaaatatc cctgtactca ctgaaaagtg ccaacaggaa gaatgtatct ccgtcttccg
     9601 cagttttaaa agctatcgca gtggcgaact ggtacgcaaa gaggaaaagg aatgtatggg
     9661 aaacaccctc tatatcggtt cattacaaag tgaagtttat ttctgtatct atgaaaagga
     9721 ctacgagcag tacaagaaaa atgatattcc cattgaagac gcagaagtaa aaaaccgttt
     9781 tgagattcga ttgaaaaatg agcgtgccta ttatgcagtc cgtgatttac tcgtctatga
     9841 caatccagag cataccgcct ttaaaattat caatcggtat atccgttttg tagataaaga
     9901 cgattccaaa cctcgttctg attggaaact gaatgaagaa tgggcttggt ttattgggaa
     9961 caatcgtgaa cgattaaaac taaccacaaa accagagcct tactccttcc aaaggacgct
    10021 gaactggcta tctcatcaag ttgccccgac cttaaaggtt gcgattaaac ttgatgaaat
    10081 caaccagacg caggttgtaa aagacattct cgaccatgcg aaactgacag accgacacaa
    10141 gcagattttg aagcaacagt cagtaaaaga acaggacgtg ataacaacaa aaaaataact
    10201 caaatacaaa ttcat
//
LOCUS       DACCCS010000085        10103 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.089, whole genome shotgun sequence.
ACCESSION   DACCCS010000085 DACCCS010000000
VERSION     DACCCS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.089"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            54..887
                     /locus_tag="IVK82_002480"
     CDS             54..887
                     /locus_tag="IVK82_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP6393512.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            903..1640
                     /locus_tag="IVK82_002481"
     CDS             903..1640
                     /locus_tag="IVK82_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP6393513.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(1795..2763)
                     /locus_tag="IVK82_002482"
     CDS             complement(1795..2763)
                     /locus_tag="IVK82_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP6393514.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            2995..3828
                     /locus_tag="IVK82_002483"
     CDS             2995..3828
                     /locus_tag="IVK82_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP6393515.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            3862..4701
                     /locus_tag="IVK82_002484"
     CDS             3862..4701
                     /locus_tag="IVK82_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6393516.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            4727..5287
                     /locus_tag="IVK82_002485"
     CDS             4727..5287
                     /locus_tag="IVK82_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP6393517.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            5486..7207
                     /locus_tag="IVK82_002486"
     CDS             5486..7207
                     /locus_tag="IVK82_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP6393518.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            7372..8514
                     /locus_tag="IVK82_002487"
     CDS             7372..8514
                     /locus_tag="IVK82_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP6393519.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            8530..9741
                     /gene="thiI"
                     /locus_tag="IVK82_002488"
     CDS             8530..9741
                     /gene="thiI"
                     /locus_tag="IVK82_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP6393520.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         3457 a         1703 c         2124 g         2819 t
ORIGIN      
        1 cattaaaaat atgatgacga agtagaataa aattgggagt aggggggaaa gtcatggata
       61 tccaaactgt tttatctgat ctgaaattaa atggtaaagt gattccagtt gttggtggcg
      121 atgttaatca gacctatcgt ataaaaaccg aacatcgtgc ttatttttta aaaatccatc
      181 cgaatgtaaa aaaaggattt tttgaagcag aagtagacgg attaaaagaa ctgtcagcat
      241 tcgtacgtgt accggatacg tatatgctag gtgaaacaag tgaaggcgct tatttgttaa
      301 tggaatggat cgagccggga aaaggggatc aacgagatct tgctgcagct ttagctaatc
      361 tccatcagca aacagcgcca caatttggtt ttcgtaaaga taactatttg ggtacacttg
      421 ttcagaaaaa ttcttttgaa gaagattggt ggactttttt ctttaaagat cggcttgaat
      481 cccagatttc tttggcagaa gaaacgaatc gatggaatgt acagcgtcaa gaaaagtatc
      541 tgcgatttaa agaacgtgta ttgaaatcgg ttgaaccaaa aaagatcact ccacgcctct
      601 tgcacggaga cctgtggagt ggtaatgtgt tctttgacca gcaagggcat ccagtttttg
      661 tcgacccagc tgtttcttat gggaatcggg aacaagatat tgcaatgagc cagttattcg
      721 gtggtttccg accggaattt ttagatgctt atcaaactat atttccatta gaaaagggat
      781 ggaaagaccg actgccgatt taccagttat attatctttt ggcacatttg aatatgtttg
      841 gagaaagtta cggttcccaa gtagatcaat tactagaaaa tttttaagag gagggcgtgt
      901 ttttggcaaa aaaatatacc aaaaagcatg aagtttcata ttatgagtgt gacatcaacc
      961 aaacgatgac atttccttcg atgttaggag tagttatcaa aacgtctgaa gatcaaagcg
     1021 accagttggg aagaggcagt gaattcgtca atagctttgg acttacttgg gtcatcacga
     1081 attattccat ggagattaat cgtctcccta gagtcggtga agtaatctca gtaacgactc
     1141 aagccatgga gtacaataag tttttctgtt atcggaactt ctggatccat gatgaagatg
     1201 ggaaagaatt agtcaaaata gaatctgttt tcgtattgat ggattttgtg aatcgaaaaa
     1261 tgagcagtgt taatgaagag atcattgctc cttttgagag tgagaagatc aagaaaatca
     1321 agcgtcagga aaaaattgaa aagattgatt cgggcttcat gttgccatat cgtgttcgtt
     1381 tttatgatat cgatagtaat cagcatgtta ataatgcgat gtattttaat tggatcattg
     1441 acgtattagg atatgacttt ttgactacgc atgttccaga aaaagtaatt attcgatttg
     1501 ataaagaagt agagtacggg aatgagatcg aaagccattt tgaacaaatc cctatcgaaa
     1561 atggtgtcaa aacaaaacat gaaatccgaa tgcaagaaca gctgtattgt gaagcaaata
     1621 ttatatggaa aaataaataa aaaagaggtt gtgacagaag ggggcaactt caagaaataa
     1681 gaagaaattt acgaaaattg tttctcaaat ttttgtaaat tttcgactta gttttcgaag
     1741 aagctatttt tgtcacagcc tctcttttta agtttagcta ttagataatg atgtttagtt
     1801 ttggataaac gaaatcaatt cttcacccga caaaccatag tttacagcaa tcaatagttt
     1861 taaacgtgct ttttgactat taatactgtt acagaaaata atccccattt tttctagttc
     1921 ttttcccccg ccttcataat cataaacagg ctctgcgatt ccattgaaac aacgagaaac
     1981 aagtactaca gggatttttt ggacaagaag cttttccaaa gcagctaagg cttgtggagg
     2041 gagattcccc gcacctaaag cttcgattac caaaccgtct actttcgtat gggcgatggc
     2101 ttccaataag tccccttgca tcccagcata tgctttaacg atcgggatag tcccatctac
     2161 agcggaaatg tccagtcgct ttgtttctaa aagttcctgc aagaataaaa ttcgattctt
     2221 agttactaag ccgatgggac ccaatgtagg tgttcggaaa gtagcaacat ttgtggtatg
     2281 ggtctttgtc acgtagcgtg cagaatggat ctcatcgttc atgaccacca aaaccccctt
     2341 atctagcgct tcttcacaac ttgcgacacg aatcgcactt tcaaaattat aaaggccgtc
     2401 acttccaagt tcattacttg aacgcattgc cccagtcaaa acaattggta atttgttgcc
     2461 aatcgttgta tcaagaaaaa aagcagtttc ttctaatgta tctgtaccgt gtgtgatcac
     2521 cactccggat atacctgatg cataagcgga caagatacgt tcttttaata ccaacatgtg
     2581 agaaggcatg atgtgtggtg agggaagatt gaagatatct tcaactaata attctacatg
     2641 cttggggatt gtgatttctg catctaataa tgggtttgcc acatcaggac taacaccgcc
     2701 cgtgagcatg ttttctttca tggcgatcgt tccgccggtg tgtagtacaa gtattttttt
     2761 cattttacag acttccttac gaataatttt gcatagccgt taaatcttat tgtatactat
     2821 ataaaagagt tttgctatca gtgaagaata acgaatagcc taacttatca taaaaaagct
     2881 gaccttatta aaatggaaaa gactcaacct tgacctccta atacaatgga gtatctggtt
     2941 gagtttcttt tacaagaaag cagccaagga aatcaatcac gaaggtggcc ggaaatgatc
     3001 agaatcggat taacatcgtt taaagaacat gacaaattaa ctggtaaaaa gaataacact
     3061 ctttatgaat atgcagggtt tctaccctta gtggaattag atactgccta ttatggaatt
     3121 ccccgaaagt cctcagttga gaattggata agtgaagtac ctgaatcatt tcgctttgta
     3181 gtcaaagtct atcaaggaat cagcggtcaa ggagactgga agccttatta tcagaccgaa
     3241 gaagagatga ttgcgcattt tttagatgtt atgtcgccaa tggtagatgc tggaaagatg
     3301 ttttgctttt tagtccaatt cccctcatca ttcaaatgta cgaaagaaaa tgtccagtac
     3361 ttgcgcaaac ttagaaagtg gtttgagggt tatcctgttt ctatagaatt gcgggattat
     3421 tcttggtaca gcgatgaatt tcttgaacgt acaagaaact ttatgtctag agccgctttt
     3481 tctttagcca tcatcgatga gccacagttg ctgaatacaa cgattccttt tgatccattt
     3541 gtcaccaatc cgaactttgc tctttttcga ttccatggta ggaatgcgca aggatggcaa
     3601 gcaaatgata aagagtggcg taaaaaaaga acgttatatc gttattcaga aaaagaactg
     3661 caagaactag cacatgaagt caaagtagtt gattctaaga caaaagaggt aggtgtgatt
     3721 tttaataata attctggtgg tgatgcggct gaaaatgcat tagcactgca aaaaatatta
     3781 aatatcaagc cagatggact caatccaacc caaatggatc tgttttaagg gcttatttta
     3841 ttcaagaaag gggcgaagtc tatgatcaaa gctatttttt ttgatattga cggaacattg
     3901 gtcaccaatc gttcaaaagc gttggaatca acaaaacaag caattgaata tgctcggaaa
     3961 aacggcattt tatgcggtgt agcaacgggg cgcagcccag taaagatcaa agaaatcatt
     4021 gatgaattgg aattagatat gtatgtcgtg tataatggtc aactggtttt tactgcagat
     4081 cgcacgatta tcgaccaccc ttttgaacaa aaagtattgg aacatattgt tgaatttgca
     4141 gatgagaacc atcgacaaat cgtttttggt gcgagaaatc ggttagatgg atcaacgacg
     4201 atgctattag gacaatcgat ttttatcaag cgactagtca gctttttacc gcgtaaattc
     4261 ccggtacgtc tgatgaaaaa aatcctccag gtttttagcc ctcaccgaca aaaggatcgt
     4321 tatgaaacgt tagagatatt aaaagaacca atttatcaat gtatattgct cagtccggag
     4381 tcagaacaga aaaagttaga acatcgcttt cccgagtgta cgtttcagcg ttcaaatacg
     4441 tatactgtag atatcatccc aaaaggtggt tcaaaattac taggcatcca agctttcgca
     4501 aatgctgtcg aaattgaaat ggaagaaatt atggcattcg gcgatcatta caatgatatc
     4561 gagatgctga aaggtgtagg aattggagtg gccatgggga atgctcagat cgaagtaaaa
     4621 catgctgcag actttgtgac tcaatcaaat gaagaagatg ggatatacga aggactgaaa
     4681 cacttcggtc tgatttatta gtcaatgaaa gaaggaaaaa atcagtatgg aagcagagac
     4741 accttttcaa aaagtagaaa cattccacaa aacttttgat ccaagaagac cagagatacc
     4801 cacggctttt acagaaaatg aagctcatac acgtgctgcg ttcaaaatag aagaacttgt
     4861 tgaatttgtt gcagctactt cacctaataa agaacaactg catgaagctg ttatgctgct
     4921 gcacgaagca ttagataaag cagaagaaaa aatacttggt aaaacacagt ggggaagtga
     4981 tccgctggtt gaacaagtag atgcactgac agatatcctt tactttactt atggttcttt
     5041 ttcattacta ggaatagtgc ctgatcgaat attcgaaatc gtccatcagg caaatatggg
     5101 gaaattattt ccagatggaa aacctcatta tgaccctgta acaaataaag tgttgaaacc
     5161 tgaaaactgg cagatggaat atgctccaga acctaaaatc gcggcagaaa tccaacgtca
     5221 gattcaagaa gcgattgcta agaagaaatc cgctgatata ggcatgtgca caaatgaaaa
     5281 agagtgatca gagtttcact atttttgtta tctttatacg atggaaaaac aaaagatttt
     5341 cgtttaagtt agtgaatgca tgaaaatagt atgctataat ctagaataga tgtcttttgc
     5401 acgagagacg tattatttgg atttatgcca agcagaacgc catctgttgc ataaaagatg
     5461 catactgaaa acggaggaac cataaatggg aaataatgtt atcatcggaa tcgtcattgc
     5521 aatcattata gtcgccattg tcgtcttttt ggttagttac ttgatgaaga aaaaaaacca
     5581 agatcgattg gatgccttag aaaaacgcaa ggaagaatta tttgacttgc cagtgattga
     5641 agaagtcgat gatgttaaaa aaatgcatct ggttgggcaa agccaaaata cctttcgtga
     5701 atggagtcaa aaatggaatg acatatctac ttcctcgttc gctgaactag aaagtcaaat
     5761 tttcgaagtg gaaaacctca atgaaagttt tcggttctta aaaggaaaaa aagcagttgt
     5821 tgaagcagaa gctacaatgg accaaatgga gaaacaagtg acggaaatcc gtgaaggtct
     5881 aaaggaattg cgggaaacgg aagaacgcaa ctcactagct gttcagcagg cacttgacgt
     5941 ctatgaagaa ctgaaagaaa aagtcagaga agacgagcat gaatacggac cagctctttt
     6001 tgaattgcaa aaacagatca aagtgatcga aaaagaattt acgcagtttg tgactttaaa
     6061 cacttctgga gaccctgttg aagcccgcga ggttttggat aaagcagaaa aacatacgta
     6121 tgaagtggaa gctctgatga aaaaaattcc accgctgtat gaggagctcc ataccacttt
     6181 ccctgaacaa cttgaagaaa tcagtgatac ttacgagaaa ttgatggaag aacaatatgt
     6241 attcccagaa gaagatcttg ctgaagatat cgcaaaagta agtcgaagaa ttgaaaattc
     6301 attagccaat cttgaaaaaa cagaagttga aacggtcgaa tttgaaaacc gtgaaacagc
     6361 tgatttgatt gattcgttat atgatatctt agaacgggaa atggaagctc agagatatgt
     6421 aaaaactaac caatcaacga ttgcagagta tatcaaacat acaacaaaga ataaccgtca
     6481 attattgatt gagctggatc acacatctca aagctataca ttgaaccaca atgaaattgg
     6541 ccgtgtacga ggtttccaaa ccgaagtgga agaaatggaa cgtcaaaatg aacagatgat
     6601 tccacagatc cgtcaacacg aaattcctta ttcagaagtg cgcacgttct ataaaacagt
     6661 cttcaaagtc ttagaagata tcgaaaagca acaagtagag atcgacgact cattacatga
     6721 attgagaaaa ggcgaaaaag aagcgcaaga aaagatcgat acattcgaat tcaaattgcg
     6781 cagtttgaaa cgttttgtag aaaaacaacg tttgcctggc ttgccgaatg attacctaga
     6841 attctttttt gttgctactg accgtattga agaactaagt gttgtcttga ataaaattcg
     6901 agtaaacatg gaagaggtca atcgtttagt tgctctatgt gaagaagatc tagaattatt
     6961 agataagaag acacatgatt tagtagatgc agcagcatta acggagcaaa tgctccaata
     7021 tgcaaatcgt tatcgtcata cacacgaaga agtgagagaa gcaattgata aaagctacta
     7081 tttattcaat aaagaatatc actatcaaga agcattagac gaaatcggta cagcattaga
     7141 gcgagtagaa cctggtgctt tcaaacgcat cgaagatttt tattttaatc atccagactt
     7201 agtataaagc aaaaaagtta gtaaaccagc aagagcaaga cggattattg tcttgttctt
     7261 gtcttttttt gtatatttga aaattgaaac aagtgaaaat ttcacctgct cttgaaagca
     7321 ccttggacaa ccgttataat agaaaagaat ttcaaaaaag aggggtttct tatgatatat
     7381 tttgataaca gtgcaacaac cccaatcttt ccgcaaagct tagatgcata tgtaaaaacc
     7441 agtcagcgga ttattgggaa tccatcaagc ttgcatgatt taggcaatca agctaaccgg
     7501 ttgttgcagc aagcaagaaa gcagatcgct gatttgatcc atgtagaacc agaagaaatc
     7561 tattttactt caggcggaac agaaggagat aactgggtat taaaaggaac gatgatcgaa
     7621 aaaagagaat atgggaatca tatgatcatt tcaggagtcg aacatccggc cgtcagcgag
     7681 acagcagaac aattaaaaga attaggaatc gaagtaagta tcgccccagt ggataaacgc
     7741 ggatttgtac gtgtcgacga attgaaagaa ttaataagaa aagagacagt attagtctct
     7801 gttatggctg tgaataatga agtaggatcg attcagccca tccaggaaat cagtgatctt
     7861 ttggatgatt ttcctaaaat tcatttccat gtagatgctg ttcaggcgat tggaaaagta
     7921 ccattctcgc aatggttgac agaaagagtt gattttgcaa cattttcagc tcataaattc
     7981 catggcccac ggggaactgg tttcatttat tggaaaaagg gtcgtcgttt agcaccatta
     8041 ctgacaggtg gagggcaaga aagaaatcag cgaagtggga cagaaaatgt tccaggtatt
     8101 gtggctatgg ttaaagcttt acgtttatca ttagagataa aggaacaaaa acctgagcag
     8161 acacaacgct taaaagtagc tctaatcaat gcgttgaaga cgtatgagaa agtgacgatt
     8221 ttttcagaag gaactgacta tgcaccgcat attttatgtt ttgctctaaa aggaattcgc
     8281 ggcgaagttc tcgtccatgc cttagaagaa aaacaaatat ttgtttctac tactagtgcc
     8341 tgctcctcac gtaaacatat ggcaagcagt acattgcacg caatgcatgt accaaatgat
     8401 ctggctacat ctgctgttcg aatcagctta gatgaaaaca attcgcttgc agaagtcgag
     8461 caatttatga ttattttcaa gcagctttat aagaaatttt ctgcaatcaa ttaaaagagg
     8521 agtgttcttg tgaaatacag tgaaattatg gtccgttatg gggaactttc gacaaaagga
     8581 aagaatcgaa aaagctttat catgcaacta gcacaaaatg tcagacaagc tttgaaagac
     8641 tttccagaaa tcaaaatcca tgctgatcgg gaccggatgc atcttttatt gaatggcgca
     8701 gacgggcagc tagttattcc aaaactagca aaaatatttg ggatccaaaa tttctctcca
     8761 agtattcgtg tagaaaaaga tgtcgaagtt ttgaaacaag ctgttcaaga catcatgaaa
     8821 gaaatttata ctgggaaaga aacatttaaa attatcgcta aaagaagcga tcaccaattt
     8881 gaattgacaa gtaatgaatt gaatcaaaca ttaggtaatg ctgtttttga tattttcccg
     8941 catatccaag ttcaaatgcg tcaaccagat attccattac gtgtagagat tcggcgtgat
     9001 ggcgcttatc tatcgtatga aacgatcaag ggagctggag gattgcccgt aggaacaagc
     9061 ggaaaaggaa tgcttatgct ttctgggggg attgattctc ctgtagcagg gtactttgcc
     9121 atgaagcggg gagtggaaat tgaagctgtt cattttgcta gtccgccgta tacaagtgaa
     9181 caagcactgc aaaaagcaaa agatctaaca gccaaactag caccttatgt gggaacgatc
     9241 cattttatcg aagtgccatt taccgagatt caagaagaaa tcaaaaagtc agtgccacaa
     9301 ggatattgga tgacaattac tcgtcgaatg atgttgcgtt tgaccgatga aattcgccgt
     9361 attcgtcatg gtttagtcat tttgaatggt gaatcattag gtcaagtggc ttcacaaact
     9421 ttgcaaagta tggtggctat caatgaagta acgaatacgc caatcattcg gcctgtagcg
     9481 acaatggata aacttgaaat cattgaagta gcagaacaga tcgatacatt taatctttct
     9541 atccagcctt ttgaagattg ctgtacgatt tttgctcctc cacagccaaa aacacgccct
     9601 caactggaaa aggtccatca atatgaagag cgtttagcaa tagatgaaat gatccaacga
     9661 gcactagctg gcttgaagat ggaagagata caagcagaat cgtctaatga aaaaactgaa
     9721 gaattttcag aattgctata agaggttatg aaaatagtgt tttgaccaga gtattagaga
     9781 aacaaaacga aaaaatagct ccatgtattt taccgttttt gaactaatgt cgaagattag
     9841 ctgattgaat accatttatc aagaagttat ggaaaagccg gttctttttt actaactgaa
     9901 aaataaaatt ttatttagag atcaagctac agttgataaa tgatataaaa aagagaattt
     9961 cccatgaaat agcgaggttg tgacagaagt gggcagcttc aagaaataag aaggaattta
    10021 cgaaaattgc ttttcaaatt tttgtgaaat ttcggcttat ttttcgaaga agttacttct
    10081 ggaacaccgt gtatcagagg ttg
//
LOCUS       DACCCS010000086         9964 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.090, whole genome shotgun sequence.
ACCESSION   DACCCS010000086 DACCCS010000000
VERSION     DACCCS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.090"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            8..81
                     /locus_tag="IVK82_002489"
     tRNA            8..81
                     /locus_tag="IVK82_002489"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IVK82_002490"
     tRNA            101..173
                     /locus_tag="IVK82_002490"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IVK82_002491"
     tRNA            201..274
                     /locus_tag="IVK82_002491"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IVK82_002492"
     tRNA            288..361
                     /locus_tag="IVK82_002492"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IVK82_002493"
     tRNA            385..474
                     /locus_tag="IVK82_002493"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IVK82_002494"
     tRNA            487..560
                     /locus_tag="IVK82_002494"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IVK82_002495"
     tRNA            579..654
                     /locus_tag="IVK82_002495"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IVK82_002496"
     tRNA            656..726
                     /locus_tag="IVK82_002496"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IVK82_002497"
     tRNA            740..813
                     /locus_tag="IVK82_002497"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IVK82_002498"
     tRNA            838..925
                     /locus_tag="IVK82_002498"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IVK82_002499"
     tRNA            947..1018
                     /locus_tag="IVK82_002499"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IVK82_002500"
     tRNA            1041..1130
                     /locus_tag="IVK82_002500"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IVK82_002501"
     tRNA            1143..1216
                     /locus_tag="IVK82_002501"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IVK82_002502"
     tRNA            1235..1307
                     /locus_tag="IVK82_002502"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IVK82_002503"
     tRNA            1311..1383
                     /locus_tag="IVK82_002503"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IVK82_002504"
     tRNA            1388..1458
                     /locus_tag="IVK82_002504"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IVK82_002505"
     tRNA            1471..1544
                     /locus_tag="IVK82_002505"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IVK82_002506"
     tRNA            1556..1627
                     /locus_tag="IVK82_002506"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IVK82_002507"
     CDS             1893..3086
                     /locus_tag="IVK82_002507"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP6393521.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQIAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IVK82_002508"
     CDS             3216..4688
                     /locus_tag="IVK82_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6393522.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IVK82_002509"
     CDS             4771..5559
                     /locus_tag="IVK82_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP6393523.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IVK82_002510"
     CDS             complement(5626..6294)
                     /locus_tag="IVK82_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP6393524.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IVK82_002511"
     CDS             6409..8121
                     /locus_tag="IVK82_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6393525.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="IVK82_002512"
     CDS             8124..9896
                     /locus_tag="IVK82_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6393526.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2901 a         1780 c         2278 g         3005 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agatagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggca
//
LOCUS       DACCCS010000087         9846 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.091, whole genome shotgun sequence.
ACCESSION   DACCCS010000087 DACCCS010000000
VERSION     DACCCS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.091"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            225..1082
                     /locus_tag="IVK82_002513"
     CDS             225..1082
                     /locus_tag="IVK82_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP6393527.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1197..2117
                     /gene="pstC"
                     /locus_tag="IVK82_002514"
     CDS             1197..2117
                     /gene="pstC"
                     /locus_tag="IVK82_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP6393528.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2117..3001
                     /gene="pstA"
                     /locus_tag="IVK82_002515"
     CDS             2117..3001
                     /gene="pstA"
                     /locus_tag="IVK82_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP6393529.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            3016..3822
                     /gene="pstB"
                     /locus_tag="IVK82_002516"
     CDS             3016..3822
                     /gene="pstB"
                     /locus_tag="IVK82_002516"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP6393530.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3838..4596
                     /locus_tag="IVK82_002517"
     CDS             3838..4596
                     /locus_tag="IVK82_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP6393531.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4609..5286
                     /gene="phoU"
                     /locus_tag="IVK82_002518"
     CDS             4609..5286
                     /gene="phoU"
                     /locus_tag="IVK82_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP6393532.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5509..6540
                     /locus_tag="IVK82_002519"
     CDS             5509..6540
                     /locus_tag="IVK82_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP6393533.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6537..7304
                     /locus_tag="IVK82_002520"
     CDS             6537..7304
                     /locus_tag="IVK82_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP6393534.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            7404..8972
                     /gene="liaX"
                     /locus_tag="IVK82_002521"
     CDS             7404..8972
                     /gene="liaX"
                     /locus_tag="IVK82_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP6393535.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9151..9456
                     /locus_tag="IVK82_002522"
     CDS             9151..9456
                     /locus_tag="IVK82_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP6393536.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF"
     gene            9456..9806
                     /locus_tag="IVK82_002523"
     CDS             9456..9806
                     /locus_tag="IVK82_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP6393537.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
BASE COUNT         3170 a         1678 c         2110 g         2888 t
ORIGIN      
        1 ttaactttgg ggtatctctt gttttttggg gaaagcaaga aataagctaa tatatgctat
       61 taaaagagac acatttcaaa gccagtatag catagaaact tttaatgaga tagttttttt
      121 tacaattatg agaatttatt ttgttattta atgattttga ttcgttttca tcaagtaatg
      181 aaaatgataa aataaacata ctttaggatt aaaggagatt tctaatgaaa aaacttaaat
      241 tattcttacc aataatagga ttagccattc ttttatcagg ctgttcaaag tggattgatc
      301 gaggagaatc cattacggct gttggctctt ctgctttaca gcctttagta gaaacagttg
      361 ctgaaactta ccagacagaa aatcctggaa aattcatcaa tgtacaaggg ggcggaagcg
      421 gaactggttt gtctcaagta cagtcaggag cagtcgacat cggaaactct gatttgtttg
      481 cggaagaaaa atcggggatc gatgcctcag cattagtcga tcatcgagta gcagttgttg
      541 ggatcactcc tattgtgaac aaggatattg gcgtaaaaga catcagtatg gaaaacctga
      601 aaaaactatt tacaggcgag atcaaaaact ggagtgaagt cggtggaaaa gatcagccag
      661 tagttatatt gaaccgcgct tcaggaagcg gaacccgtag cacgtttgaa aaatgggtat
      721 tggacggaga aacagctgtt caggcacaag aacaagattc aagcggaatg gtgcgccaga
      781 tcgttgccga cacacccggt gcaatcagct atgttgcttt ttcttatgtt tccgatgatg
      841 tagatacgtt gaatgttaac ggtgtagaac cgacagatga aaatgtgaca acgaacaaat
      901 ggacgatttg ggcatatgag catatgtaca caagagatca tccaaaaaaa ctgacaaaag
      961 aatttttaga ctatatctta tctgatgaag tacaagataa catcgttgga gaactaggtt
     1021 atatccctgt ttctcatatg aaagtcgaac gtgattggca aggaaatatc gttaaagagt
     1081 aatctttaca caaaatttac aaacttagct aaacttttac atgttattaa aaccaaattg
     1141 atgttcaatt tgtacactga gtgagtatgt taaacctgat taagggggag tcatctgtgg
     1201 aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc aaaaatggaa cagcgtggaa
     1261 aattcatcag ttttttatgt atcgcgctga tcgtgttggt tgtattatct attttttatt
     1321 ttgtagctag taaaggattg gccacctttt ttactgataa gatcaatgta tttgattttc
     1381 tgtttggtac agattggaat ccaagccaaa tcggagcaaa tgggaaacca atggtagggg
     1441 cattgcctat gatcacaggt tcgtttattg tgaccttttt atctgctatc gttgccacgc
     1501 catttgccat tggtgcagca gtattcatga cggagatctc tcctaaaaac ggaacgaaga
     1561 tcttacagcc ggtcatcgag ctgcttgtag gtattccttc tgtcgtttat ggttttatcg
     1621 gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg cgggacaggg tttggtatct
     1681 tagctggtac atttgtgttg ttcgttatga tcttgccgac tgtcacaacg atgactgttg
     1741 atgcattaaa ggcagtacca cggcactatc gggaagcttc attagcgctt ggtgccacaa
     1801 gatggcaaac aatctacaaa gtcgtgctac gagcagccgt gcccgggatt ttaacagcag
     1861 tcgtttttgg tatggctcgt gcgtttggtg aagccttagc tatccaaatg gtcatcggta
     1921 atgcagcatt gatgccaaca ggacttacta ctcctgcttc tacattgacg tccatcttaa
     1981 caatgggaat cggaaataca atcatgggaa cagttgaaaa taatgtgtta tggtcattag
     2041 cgctgatctt gctattgatg tcattattct ttaatatcgt gatccgtatg attgggaaga
     2101 aaggagagct gaaataatga acgcaaaacg ctcagataaa attgctacag gtgttttata
     2161 tatagtttcg gggatcatcg tattgatttt agcagcttta ctactttata tcttagttcg
     2221 gggaataccg cacatttcat ggtccttctt gaccgaacct tccaaagcct accaagaagg
     2281 tggtggaatc gggatccagc tatttaactc gatttatttg cttttgatca cgatgatcat
     2341 cagttttcct atttctttag gtgcgggtat ctatctttca gaatatgctg gaaaaaactg
     2401 gctaacagat gtgattcgca cgtcgattga aatcttgagc tcgttgcctt ctgtcgttgt
     2461 cggtttattc ggtttcttgg ttttcgttat tcaattccag tatggtttct cgattatttc
     2521 tggtgcattg gctttgactg tattcaactt gcctcttttg actagaacag ttgaggagtc
     2581 tttacaagcg gtccattaca cacaacgaga agcaggattg gccttaggtt tgtcacgctg
     2641 ggaaacagtg atgaaagtgg tggttccaga agcgacacca gggattttga ctggtgtgat
     2701 cctaagttct ggaagaattt ttggtgaagc agctgcattg atctatacgg ctggacaaag
     2761 tgcacctgcg ttagatttta cgaactggaa tccattaagt gtttcaagcc cgatcagcat
     2821 tttccgtcaa gctgaaacat tagctgtcca tatttggaag atcaatacag aaggcacgat
     2881 gcctgatggt gtttctgttt cggctggcgc ttctgccgtg ttgatcattg ctgttttact
     2941 gttcaacttc ggcgctcgtg cgattggcaa acgattatac cgaaaaatga cttcagccta
     3001 aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa caaatgtcat caccatgcca
     3061 aaagagaaag aagtagcact atatacggat gatatacatg tctggtatgg cgacaatgaa
     3121 gcaatcaaag gagtggatct ggaatttgaa aaaaataaga tcactgcact gattggtcct
     3181 tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc gaatgaatga tgggatcgct
     3241 aatacgaaaa tcactgggaa aatcatgtat caaggcgtag atattaatgc gcctcaagtg
     3301 gatgtttatg agatgcgcaa acggattggc atggtttttc aacgtcctaa tccgttcagc
     3361 aagtcgattt atgacaatat tacatttgct ttgaaacagc acggcgaaaa aaacaaaaag
     3421 aaattagatg aaatcgttga aacaagcttg aaacaagctg ccttgtggga ccaagtaaaa
     3481 gacagcttgg acaaaagtgc cttggcttta tctggcgggc aacagcaacg tttatgcatt
     3541 gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag acgagccggc aagtgctttg
     3601 gaccctattt cgacaggaac ggttgaagaa acacttgtaa acttgaaaga agactatacg
     3661 atcatcatcg tgacgcataa catgcaacaa gcagctcgta tcagcgacta tactgccttt
     3721 ttctatctag gaaaagtaat ggaatatgac aaaactaaca agatatttac cagaccaaaa
     3781 attcaggcaa ccgaagatta tgtgtccggt cattttggtt aagacggagg cctcaacatg
     3841 acaaaagaaa ttatttcatc taaagatctt catctgtatt atggaaagaa agaagcacta
     3901 aaagggatcg atctaagttt caatcaaggg gaaatcactg cgatgatcgg tccttctggt
     3961 tgcggaaaat ccacttatct gcgttctttg aaccggatga atgacctgat tccgaatgta
     4021 acgatcaccg gaagtgtttt atataaagac aaagacatct atggtccaaa aacagatgtt
     4081 gttgaactaa gaaaagaaat cggcatggta ttccagcagc caaacccatt tcctttttct
     4141 atctatgaaa atgtcatcta tggattgaaa ttaaaagggg aaaaagataa aaaagtcctt
     4201 gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt gggatgatgt gaaagacaaa
     4261 ttgcataaaa gtgctttatc tttatccggc gggcagcagc agcgtgtatg tatcgcacgt
     4321 gttttggcag ttaatccgga aatcatctta ttggatgaac caacaagtgc gcttgatcct
     4381 gtttctactg ggaaaatcga aagtatgctg ttagaattaa gagagcggta tacgatgatc
     4441 atcgtgaccc ataatatgtc tcaagcttca cgtatttcag ataagacggc attcttttta
     4501 gatggtcact tgatcgaata caacgatacg aagaagatat tcatgaatcc agaaaaacaa
     4561 gagacagaag attatatttc aggtcgattt ggataaggag gaaacggaat gctacgtaca
     4621 caatttgaag aagaattgct gaatctccat aatcagttct acgaaatggg gatgatggtg
     4681 agcagcgccg tgcacaagtc agttcgtgca tacatcaaac acgataaaat cttagcccaa
     4741 gaagttatcg aaaacgatat aaatatcaat aacatggaaa cacgcttgga gaagaaaagt
     4801 tttgaaatga tcgcattgca acagccagtt acaactgatt tgcgtatgat cattacggtc
     4861 atgaaagcta gctcggattt agaacgtatg gccgatcatg cggtgtctgt tgctaaatcc
     4921 acgattcgcg taaaagggca gacgagaata ccggaaatcg aaaaagagat ctccgatatg
     4981 tccgattatg tgaaaaaaat ggtggacaac gtcctagttg cttatgtcaa aacagacgaa
     5041 aaagacgcac gtacgatcgc caatatggat caaagagtca atgactattt caatcgaatc
     5101 tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga cagtcatcag tggaacagac
     5161 tacttgaatg ttgccagtta tctagaacgg atcggagact atgtgacaaa catctgtgaa
     5221 tggatcgtct acttggctac agggaaaatc actgagttaa atacgaacca caatgagatt
     5281 ttctaaactg aagctgtgat tatgccgttt gtttatgaaa agaggttgtg aaaaagctgt
     5341 tctacatcaa gtaataagaa cagtcaaaca aaaaatagct cctcatattt ttcgtttggc
     5401 tggacttatt accgcagatg caagcttttg aacaccgttt attcgggaaa gggtttgtga
     5461 caaaacatgt cacaaaccct tttcatcatt tcatagaaag gataaagaat gcaaaaaaaa
     5521 attagcatca tcactatatt ttttatttgt gtgcttactt catgggttat tcagcatcaa
     5581 gccgctttat atgaagtacc tgtcatcgag atagaaaaag tacgaataaa agaggatcat
     5641 caattgatta caggaaaact gatgaactcg gatcaaaaag gagaatcgat ttatttatcg
     5701 gttccttatc aaaaaaatca aatagaaaca gcaaatataa aagcgagaca aatgtatcta
     5761 gtccaaatgc atggagatga atggcagcta gtagcaaaaa aaaatgatgg ctggcttttc
     5821 ttcttcacaa gtatcttcat tggcgccatg ttgcttattg gcggaaaaca gggtctattt
     5881 gctttaggaa gtctcatcat caatatcgtt ttacttttac ttttgttgct catctttact
     5941 aaaacagaag ggatttctct attaaataaa tctatcctgt tcgtcgttat agggatgatt
     6001 attagcatat tggctttaga tggatggaat agaagcagta taatcaaaat ttcagctgca
     6061 gttacgagtg tttttttagc attttttatt tgtcttttga caatgaatca ttggaaagac
     6121 caaggactgc gctatgagga actgaattat ctgacacgac cgtatcgttc tgtttttctt
     6181 tctagcgtac tgattggaac agctggagga acactcgata ccgttatcac agtcattgcg
     6241 acattagaag aattgactaa gcgacaacca gaaataacag cgaaacagtt atggctttca
     6301 ggaaggcaag tgggtcgaga tgtgattggc tcgatgacca atatcctttt gttcgtctat
     6361 atcagtgggt cagttccatc gcttctttta tatttaggaa atcaatggtc tttcaaagaa
     6421 acgatagaac agcatttatc attggaattt ttacgcgtca ttgctggcag cttaggaata
     6481 gtattatcga tccccatctc ggtcctcttt tttctgactg taaggaggct gaaacgatga
     6541 atgtacttat tatacttgtt gggattttag caatcagcgt gctacttatt ggcggtacgc
     6601 aaggtatgta tattctttta ggcttattca tcaatttagg gatctttttc ctgttgttat
     6661 tcggttatca tcagaagtgg ccgatactgg cattgtcggt cataggattt ttattgattg
     6721 ctgtggttat cttatttttt atcaacggtt ataatctaaa aatgcgtgca gcttttgtga
     6781 gtatactgat ctttttattt tgtttcctac tattgcttcc tatcactgat tttttagcga
     6841 tccaaggttt tacatcggta gaactagaag aattatctgg cttagataag actttagcga
     6901 ttgattttag attacttacg agatcgttgc ttctgattag tcttagtggt gctgttttag
     6961 atgcgagtgt agcaatttct agtgggacat tcgaagtata tcaggcaaat ccgcaattat
     7021 ctttttacca attgcgccat tctagtttcg ctattgctaa aaaaatattg gcgtcaacag
     7081 taatgacact tctttttgca ttcatgggaa gttctttagc gttgatcctc tggtttattg
     7141 atttagacat tccttttcag caagtgatca atgaaaaatc atttgttcta gaatatacga
     7201 tggctattct taccacattg tccgcactaa ttgttcttcc tttaacctgc gtgactacag
     7261 catatctctt tacaaaaaag aaagaacaac tcaagatgga gtaaaagtta caacttaagt
     7321 cctatgacat ttttactgaa tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa
     7381 ttttgcttaa ggaggcaatt tccatgaaag aaagagaacg tattttagat ttagtgaaaa
     7441 aaggaatcct ttcgactgaa gaaggcttag atttattaga aagcatggca actgaaaaag
     7501 acgaaaagca aatcaaaaaa gaagcagaca aggtaactgc tagtcataaa gaaaaagata
     7561 aggcaagtca attgatcgat aaacttgaaa acggcgaaga agaaatttct gagccagttg
     7621 atccaaagga aagagaaaga caagatcaag aaaacttaga aaagatctta gatgaactag
     7681 caactgaagc gaataaaact tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca
     7741 ataaggcagc acgcaatgag aaacaggaag cattgatgca gctgaataca aaagaagaat
     7801 taggcgagtt gacagaagaa gaattagcac agcgtcaggc gttagaggca gaaatcaaag
     7861 agttagaagc tagtggcgat acactgttag aagaacaatt gaaattagaa gcagaattaa
     7921 aagatatccg taagaatcaa tggtctgaga aaaaagaaac gtttactgat aagtttgaat
     7981 tgccagatga ttggaaagat caagcaacag atacactgaa tcaagtaggc gaaaaaatgt
     8041 cagaagctgg atcacagtta ggcaaattct tgaagaaaac attccaaact gtttcagaaa
     8101 cagtgaatga caacatggaa tggaaagatg tcagcttacg tgtccctggg attgcgacaa
     8161 cgaaatttga gcatgaattt tactatgaag ctcctgcagc aagtatcctt gatatcaagg
     8221 cagcaaacgg aaacgtgacg ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta
     8281 agatcaaact ttacggaaaa atgggtgctg aaccatttga agcattctct gaacggagcc
     8341 agattgaggt taatgaagat catatctcct tccaaattcc aaataaacgt gtgcgtgcag
     8401 accttgtttt ctacttacct aaacgagtat acgaccacgc agctattaaa cttctaaatg
     8461 gcaacatcat gattgaaaca ttggaagcta aagatattta tacaaaatct acgaatggta
     8521 acatcatcgt ggaccaatta actgcaacga tgctggaagt agaaggtgta aacggcaata
     8581 tcgatattcg aaacgggaac atcttagatt ctattatcga aacagtaaat ggtacagtga
     8641 catttggtgc gacagcagaa aatctttctg tgtctttagt aaatggtgat gtacgtctta
     8701 cgattaaaga agataatttg aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag
     8761 tcgctttacc agatggcatc ggtttggaag gacatgctaa aacaagtctt ggaagtatca
     8821 atagccgtct gtccaattac gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt
     8881 tgcaattccg tcgtgtttca gatgatgaga tcgcgcaagt gcaactttca acgacaacag
     8941 gaagtatcta tttaaaagat acagataaat aaagaggttg tgaaagagat gttcagcctt
     9001 gagcaataag aagcgatttt gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt
     9061 gccgataggc tatctcttga acaccgatta agaagaaggt tgtcacaacg attttcttca
     9121 tttaaaatag tagaacagag gtgagacatg atgaaaaaaa agttgacaaa atccaataaa
     9181 aatgttgttt tgacaggtac tttagcaggg attggggaat atatcgggat tgatcccact
     9241 gttatccgtg tgctttatgt attcctaagc ttagttgctt ttggaagtcc aatcatttta
     9301 tacattttat tagctttgat tatcccatct gcgcgttctg cacgaacaaa ctatggacat
     9361 aacaatgcat attatgaaaa aaacaattat cgtgaacaaa caaaaaaacg caaagaagcg
     9421 gaaaaaatcg atgatgacga atggagtgac ttttaatgtc gtactttcag cgtattttgg
     9481 ttaatacgtt aacattcata tcgttggctg ttctttttcc tgcgatggtc catgtgagca
     9541 gtatttggac agccgtcctt gccagcttcg tattatctgt gttgaacatg tttgtcaaac
     9601 cgattctgac gatcctgtcc ctgcccttta cattagtcac attgggattg tttacgtttg
     9661 ttatcaatgc agcgattctt aaaatgactt ccttttttgt aggagcagaa cattttagtt
     9721 tttcttcatt tggtgcagcg ttattcgtag ctatcttgat gtcacttgtc aatgtaatcg
     9781 ttagtgaaca taacagcaga aagtaaaaaa agctgtgccg agatctcggc gcagcttttt
     9841 tgttta
//
LOCUS       DACCCS010000088         9223 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.092, whole genome shotgun sequence.
ACCESSION   DACCCS010000088 DACCCS010000000
VERSION     DACCCS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.092"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            53..790
                     /locus_tag="IVK82_002524"
     CDS             53..790
                     /locus_tag="IVK82_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP6393538.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            818..1357
                     /locus_tag="IVK82_002525"
     CDS             818..1357
                     /locus_tag="IVK82_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP6393539.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            complement(1855..2316)
                     /locus_tag="IVK82_002526"
     CDS             complement(1855..2316)
                     /locus_tag="IVK82_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6393540.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
     gene            2449..2823
                     /locus_tag="IVK82_002527"
     CDS             2449..2823
                     /locus_tag="IVK82_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAP6393541.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
     gene            2877..5003
                     /locus_tag="IVK82_002528"
     CDS             2877..5003
                     /locus_tag="IVK82_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393542.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQ
                     YKDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILA
                     RNDSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLK
                     KYGTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNS
                     FDTIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIV
                     TLSIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLK
                     YWERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQE
                     NNKEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTT
                     YILVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYF
                     SFNTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQ
                     INPYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIF
                     VSFTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFM
                     IFLINLITSIIIYKKNSFKYFKENIL"
     gene            5000..5620
                     /locus_tag="IVK82_002529"
     CDS             5000..5620
                     /locus_tag="IVK82_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6393543.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            6313..6693
                     /locus_tag="IVK82_002530"
     CDS             6313..6693
                     /locus_tag="IVK82_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP6393544.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            6675..7589
                     /locus_tag="IVK82_002531"
     CDS             6675..7589
                     /locus_tag="IVK82_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP6393545.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            7829..8518
                     /locus_tag="IVK82_002532"
     CDS             7829..8518
                     /locus_tag="IVK82_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393546.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(8639..8725)
                     /locus_tag="IVK82_002533"
     CDS             complement(8639..8725)
                     /locus_tag="IVK82_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP6393547.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
BASE COUNT         3571 a         1403 c         1425 g         2824 t
ORIGIN      
        1 gttttacatc ctcttatgtc tatgataaca taatttattg aggtgatttt tattgagtaa
       61 cgatatagtt cgatataata atggattaaa ttctgtccct ttaagaaaat ttacccctat
      121 agatatggat ttattttggg gaatatgttc taaaatgaaa agaaaaggta caaaagaaga
      181 aatttttaca tttaaacaaa taaaagaatt aattgattac aatataaatg cacgtggcga
      241 ccgttttgct aatgatttaa taaaaatgtc aaaaaaaatc attactttgt cttttattta
      301 tgaagacgaa aaaacatatg aacaattaaa tctgtttcaa aaatttaaaa tcgataaagt
      361 gaatgaaaca ttattaataa aggtttctga agaatttgaa tatatactta attcaatcgg
      421 aactaatttt actcgtttcg aattagaaaa tatgacaaac ttaaagagta gctacgttaa
      481 agagttatat cgccaactta tggctcataa agacagaaga tctaaaaaag gtcactggtt
      541 tgtaaaaata gaagatttta gagctctatt agcaatacca gaaagttatc gcatgagaga
      601 tattgataaa caaatttttg cccaagcgaa aaaagaattt ttaaacgtta attcaactca
      661 ccctgctatt tttaaaactt ttgaaatcaa aaaaattaaa gcctacaaag gaaataagat
      721 aggatctctg caaatttatt ttgaagaaaa tataccttca attaccttag ataattggtt
      781 agagaaataa tacctaacaa tttaggaggc aattatcatg caacccaaag aaacctttac
      841 aataaaggaa ttatctgaat tatttgaaat aagtaggcaa gcaatctcta aacatgttga
      901 taaattagac ccgtccttat tagctaaaaa tgagcgtggt tataaggttg tattacgttc
      961 tggagtattg caacttgcta gcaacttagg taaccaaagg ttactatata ggttgcatga
     1021 gtatgaacaa gaaaacgata aaaacgttta tatatcaagc aaaaacgtga ttgtaaacga
     1081 gttgctagat aagttgcgtg aagaaaatga atttttacga gaacaacttt ccctcaaaga
     1141 gcaaaataat tctaatatgc aaaaattact cgaccagcaa caacagctca ccctacaggc
     1201 aaatcaacag atcgaaaaac tgcaagaaca gttacaatta gcttatacgg aagaaactcc
     1261 agaagatgaa tcaactacta ttctttctga agaaacagaa tcgatagaaa aacacacaaa
     1321 aaagaagaag caaaaatggt ggcaactttg gaaataactg gagggaacat tcatgacaag
     1381 aaaaaatctt ctattcggtt ctattttact aggaattcta ggcttcagtc cgatgttatt
     1441 gtcagtaatc aataaaggga ataaacatgg tgaattgaaa ttgaatccaa attattacaa
     1501 cagtcctaac gaagaattgt ttgtaaaaga gacggataaa taataaaaaa gactgctctt
     1561 ccgagcagcc agttaaaaaa taatgagtgt ttattcttga aaaagcacgc gcttataaag
     1621 cgctaccaaa attttatcat attttataaa aaataacaat ccagtaaaaa atgactggta
     1681 aaaattcaca aagaaacgga aagaagcaac tacagaaaat caagccttaa aaagagcttt
     1741 ttacctagac caattaaaaa tcacaaagtg agcgtctcta tccattggta gaggggctac
     1801 ttggcatagt gtacattatg tcaactatgc aattaaataa ctaactactt catattagct
     1861 taataaaata atcaaaatca ctaggggaaa aaatattgta taaaaaaatt tttcattttt
     1921 taaataagaa tagtaaatat aaaaataatt taagatatct attactatta atccagatag
     1981 agcaaaccaa gtaaatattc cgtgtgtaaa gctaaaaaaa ttcatttgaa tcaattcaaa
     2041 taaacctaat atagtaacaa atatattaaa aataatagct gtcaatacca gtaaattatt
     2101 ttgtttattt tttaaattat cttcatttaa aatagaattt atgttagtac tataaaattc
     2161 acaaattttt tttagtgtct ctatatctgg tgttgttttg ttattttccc actttgaaat
     2221 agtttgacgt gtaacattaa attttcttgc cacatattct tgagtatacc cttttttcac
     2281 tcttaattct cttagcttag atcctaaatc ctccattttt tctcctatgt aaacgattat
     2341 ttacataaaa ttttatcata aaaatactaa cgcgccaaaa aagttcttta ttttataaaa
     2401 cacactaaaa taaaataatt ctagtaaaaa agataggaga aataagttat gaaaaaattt
     2461 ttatgtcttt ctgctttaat gggtgtctta ttaactagtg gaggaatagt tagtgctaca
     2521 gaagctttaa atctagatgt acccgaagac cataccgcca tttacggagg aggaatggaa
     2581 gctacagaag aaggattttc ttctagtaaa ttgagatatg ctgctggcgg cggagatttc
     2641 aactgtgggg ttaatggatt taaagtttat gcaaattact accatgctag agctaaacat
     2701 agtgcaacag ctaaaaatgg acgaggcggt caagtacgat cagttcagaa agctggagta
     2761 agagcttatg caacatgcaa tgccacactt actggaaata ctggttggtg gaatgtttat
     2821 taaaaaagag agtttaaact ctctttttta tttacatttg aaaggaaatt caaaatatgt
     2881 taaaaaaaat aaacgctgtt ttatcacttt ttgtctgcat tacaatacta ttcttttcta
     2941 ctaatgttat aaatggtata aagacttctg aagcgctata taaaaatacc gctgaaattc
     3001 atatgcaata taaagatgat atggacttta acagttatat aaacgctttg tatgaaatat
     3061 ctacacaaga aaatattaat atttctcaat atagttttac tgctgttaac caactaacta
     3121 ttcttgctac aaaccctaac gctaacaaac actggaaatt ttctaaaaaa aatatattag
     3181 caagaaacga ttctaaagtc cattatacta ataacaaaaa ttctaagtac caattaaaac
     3241 ttccaaataa ttttttaaat ataaatattt atcctttttc tcatgtggaa aatattggat
     3301 taagtgaaat attatatgta caaggaaact caaacaaatt aattcctgta ctgaaaaaat
     3361 atggaacacc tactatttcc aatatcaatc aatcagatac ttttcaaatt aatactaata
     3421 ttgttcttgt tatctgctat cttagtatat ttattgtaat tactacaatt atatttgctt
     3481 tttcaaaaat gaaagaaatc actctaaaaa aaatgttagg ctataactct tttgatacta
     3541 taaaagattc tattaaagag ttaaacctct tattttgttc tttgattata actttcattt
     3601 tatctatttt tttgagcata tattacagta acgaaaattt tttcctatca tttcttctat
     3661 gtagtatttt tcattttata attagttttt tatttatcat agttactcta agtatctatc
     3721 atttatctag tataaaaaat tatctaaaaa attcacgacc acttaagtta aatctttgta
     3781 tattaaatat ctgtctattc ttaagcatga tccttctgtt aatagcttct acaaaagtaa
     3841 taaatatcca taatgaagcc gaaaataatt ctcttaaata ttgggaaaga actactaatt
     3901 tatataaaac aaatattact aatcaattaa atagaaacaa caccgtagaa gaaaataact
     3961 acttgaaaaa agctagtaaa tttgtttata aaatacagaa gaattataaa acttttatta
     4021 tcgcgccata caattacgca actatccaag aaaataataa agaatttttt attggacaaa
     4081 aagtttatcc aaattttgaa gactatgtta gtcaacctgc tggtgcagga atatgtgttg
     4141 atttaaacta tctaaaatat tacaatccta tttcttttga agaatctact gacttaaatt
     4201 taataaactc tgatccacta actacttaca ttctagtacc taaaaaatat aaagaatatg
     4261 caaaaatgat tgaaaaaaat tatttagaag acatacagtt tagacttgaa gactctcctc
     4321 ctcaagctcc ttataaaact cctaatttga aaaatttaaa aataaaacta atatttgtta
     4381 ataataacca aaagtatttt agttttaata ccttatatgg aaaggcaaaa gataactata
     4441 caataactga tcccatagtt agagttatta atccagaaat aacagaatca ttattttggg
     4501 gaaatatatt gactagtgat ggtggattat tttttgatca gaaacatact tcaaaccaaa
     4561 acttttttaa tcaaattaat ccgtatataa aagaatttaa cctagaaaat atcataaatt
     4621 tttcttattc tgtttttcaa gaaactggtg aattattatc tcaaaaaaaa gtaaataaat
     4681 taaatgcata tctacaatat cttttaatat taggattatt tataataatt aattttcaaa
     4741 tgatttttgt tagttttact ataaattcaa aaaaaatagc tttaaaaaaa atatttggat
     4801 attcaccaat aagtcaagtt ttagatttat gtttactccc ttttacaata gagttatttg
     4861 ctactatgtg ttttcaattg atattttcaa aaaacactta cttgtatttt ataattttta
     4921 tgatcttttt aataaatcta attacttcta ttattattta taaaaaaaat agttttaaat
     4981 attttaagga gaatatttta tgatattatc agttgagaat ttcaccctgt caattaataa
     5041 aaaaaagatc aaacaaaatt ggaacttttc aatatcagaa aaagatttta ttatagtaac
     5101 aggaaaaagt ggtagtggaa aaactactct gttaaataat ttaagcctaa tagataaaca
     5161 atataccggt aacttattct attttgaaaa gaaagtttct aaacatgata tacaacgtct
     5221 acgaagaaat catattagct atttatttca aaactatgct cttcttgaaa atcaaacagt
     5281 acaatacaat ttcgaattag caaaaaagtt taacagtaat atttccaatg atcatattta
     5341 taatttatta acagattttt ctttgccaaa agatatctta aaagaaaaaa tctttctctt
     5401 aagcggtgga gaacaacaaa gaatagcatt aattagaaat atgttaaaac catttgatat
     5461 attatttgca gatgaaccta caggaaattt agataaagaa aactcaactt ttataatgaa
     5521 ctttctgaaa aatttaacag aaaaagaaaa aaaagccgtt gtactagtaa cacatgactt
     5581 aaatgttcta aactatggaa ctaatattat agaattataa tttttacccg tgatgctagc
     5641 tgatttaaaa ctagcaccac gggtatttat tttatattcc atctctccta tcccaaaatt
     5701 taaataatca cttaataaaa aggaggcacc caagtgattt tattgaaaaa agagtatgaa
     5761 aaattttatg atagacttat ttattctttg caattgccaa aaaaattaaa ccgtcaatta
     5821 caaaagactt atgaaactaa atcaactgaa gaatgggaga gttattgcta gatttttggt
     5881 agaagtaaca aaaagtggac atattatcat aatgactcgc cgaaagtctg ataaaaaatt
     5941 ggctaaaaat attgctgaat gtttaataaa actatcagtt ttagctaaat agttagtagt
     6001 ttacacaaaa aagcatttat gttttttgtt gcaaagcaac aatttcatct tcggtgaaat
     6061 aagcgaaact ttacaaaaag tggctagcat ttgctagctt tttttgtatt gtggagcgtg
     6121 gcttttgagg gggattcaag ggggagcttg cgcccctttg acaagcggtt atttgcacca
     6181 aatacggagg tatttggcta gcaaatatag agcttgccaa acccttttag tgagctttac
     6241 tcattgtacc tttttaggta caatggtatt gcgaacgata cgtccgtagg acgtactgaa
     6301 agggtgggac ttatgagcga acaaaatcaa aatttggcta gcaattcttc taaaaaatat
     6361 acctatcgat ccgagccaaa acaaatcagt tttcgagtga gcgaatccga atttgcaaag
     6421 ctgaagcaat cggctgaagc tttgcaaatg agtgtgccgg ctttcgtgaa agccaaggca
     6481 caaaacgcgc gtctggtgac accgaaagtg gcgccagaca ttgcccaggc aatcgcgcgc
     6541 gatttagcca aagctggagg gaatatcaat caaattgcta agtggtgcaa tacacaccaa
     6601 catgacgttg cttctggtga cgctcaacgt ttatccgaaa acctaaaaat catgcaaaag
     6661 gagctacaaa agatatggca acaattaagg taagccgaac cacttcttgt agtcgtgcaa
     6721 ttaattacgc agaaccacga gcaaccatta aaacaggaat aaactgtgac gtcgattatg
     6781 ccaaaagtga aatgaaacaa attcgcatgc tgtatggaaa agacgaccgt gtacaggcac
     6841 acttattgat tcaatctttt cgaccaggag aaattaccgc tgaaaaagct aatcaactag
     6901 gggcagagta tgcaaaaaaa atagcccccg atcaccaaat agctatttac acacatacag
     6961 acaaagacca tattcataat cacattgtca ttaactccgt taatctggag actggaaaaa
     7021 aatttcaagc tcatggtcaa gcatttctaa acaaatgtta tgacctaaat gacgagattt
     7081 gtcttgcaca taacttacca atcactgagc gaggaaagaa accagaaaaa cgaaccatgt
     7141 ctgaaataaa attaaaagaa aaaaatgaac ctgtttggaa agacgaaatt cggattgcga
     7201 tcgatcaaac aatgaaaaac aaaaaaacca gaacctatga ccaattttgt aattccctca
     7261 aaacattggg gattcattgc ttcaatcgcg ggaaaaattt cacttacgaa ttgatccaca
     7321 aaaagaaaaa agtacgatcc aacagactag gaaaggacta cgaaaaggag acgattttac
     7381 atgagctgga cagacgagaa cgaacctata accaacagcg aaccaacgag ctacgaagag
     7441 ctcgagctag cattcgcgaa actgcagaag aagtacgaag agaacaacga actcttacgc
     7501 ctgaacaacc ggaaattaga ccggctcgaa gaaaccatga acaacacgtt gaaagaggaa
     7561 cagaccacca acaagaatta tcgctctaaa acgttaaaag acatagaaaa cgaaataaag
     7621 aaacaacgtg cttttctcgt tccttatacg caagaaagga tacaaactac gattgattta
     7681 caagttaaaa aactccatca agagctatta gaccagcgaa ataaggaaca gaaaaaggtg
     7741 cttattcaac gatcattgga ttttcttgcc cccttatgtt gcttagtcct gacactgttc
     7801 gcattttggt tagtatgggg aggctaaaat ggcattttat ttaccctatc tactcatttt
     7861 tgttagtatt tcaggaagta tttggctcat ctataaaatt tttcaaacaa gatattcatt
     7921 aaaaggctct aaaattcgat ttaagcgatt tttctttctt ggctgtatat tttccttgat
     7981 aatcgtttca agcggcttat tcggcgtttt agagggaaat aagcgcgttt ctagaagtat
     8041 tttattagga aatgtcactc aaaagtatga atcagcgcgt gataaaaaga aaaaagagca
     8101 agcattagca caaaaaatgg aagagtttac cacttgttat gaggaaatga acgatatttt
     8161 tgtgaatcaa gaaaaacgtt tgacggataa aaacatggaa aaactcactc gtctttatca
     8221 aaacttgcca gaaaaacagc aaaaagaggt tcaagacaac tatgagcgaa ccaaaaaaga
     8281 cgtgcaatat gtaaaagata caaagataga agaagcctgt agtgatctat ttggagacac
     8341 aaacccttgg tttgccagtg aagaagaaaa aaaagaaaaa caacaatctg tcacatatga
     8401 acgctatgaa atcttacttc aacaagcaac taacatgcaa aatccgacaa aaaaagaaac
     8461 cgccttaaac tatctaaaaa gtgtcaagga atggttagat cagcaacaac aaaattgaat
     8521 ttgatttaaa atcatgttaa actgaatgtg caaatgatca aacattagat acacgaaagc
     8581 cccagagagg tcggattctc tggggctttt tttcaggttg atgtcgcctt tattgatttt
     8641 acttcttgtt tcggttattc aaccaataac tgaataaaga gacacaaact cctacaagaa
     8701 gggggcaaat gaacagttca aacattagat acacctcctt ccagtcgcaa gactgtcgga
     8761 taggcgacaa gttattatat cttaaaaaat taaatttgtg tggtgttttt aagtatcact
     8821 tgttttaggg ttgttagggc ttgccctgac cgcctgtaag gcgcttctcg actagattaa
     8881 gggaaattaa tcaagaattg tcttagaacg gcttagacaa gctttcagag tctcacacct
     8941 cctctttgaa caacggtttt agcaaaggag ccggaggcga ctgcgcagta taatatgaga
     9001 gcatagcgat cacacctttt tagcgaccgt agggagcaag ttttccagtt tagaaaaatt
     9061 cgcgaacctt tttggaggaa aaagtaggag ttttttaggc gggaaattca atgtgctctt
     9121 aagatatact gttagatata ctgttagata tactgttaat aataactgtt agggattagc
     9181 aaccttatgg gagagtaagt attgacatag gtagtggggg ata
//
LOCUS       DACCCS010000089         8968 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.093, whole genome shotgun sequence.
ACCESSION   DACCCS010000089 DACCCS010000000
VERSION     DACCCS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.093"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(56..349)
                     /locus_tag="IVK82_002534"
     CDS             complement(56..349)
                     /locus_tag="IVK82_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP6393548.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(377..1042)
                     /locus_tag="IVK82_002535"
     CDS             complement(377..1042)
                     /locus_tag="IVK82_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP6393549.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1182..1814)
                     /locus_tag="IVK82_002536"
     CDS             complement(1182..1814)
                     /locus_tag="IVK82_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6393550.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1821..2888)
                     /locus_tag="IVK82_002537"
     CDS             complement(1821..2888)
                     /locus_tag="IVK82_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6393551.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2885..3616)
                     /locus_tag="IVK82_002538"
     CDS             complement(2885..3616)
                     /locus_tag="IVK82_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP6393552.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3785..4264)
                     /gene="greA"
                     /locus_tag="IVK82_002539"
     CDS             complement(3785..4264)
                     /gene="greA"
                     /locus_tag="IVK82_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP6393553.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4400..5575)
                     /gene="mltG"
                     /locus_tag="IVK82_002540"
     CDS             complement(4400..5575)
                     /gene="mltG"
                     /locus_tag="IVK82_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP6393554.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5779..6948
                     /locus_tag="IVK82_002541"
     CDS             5779..6948
                     /locus_tag="IVK82_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP6393555.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(7215..8837)
                     /locus_tag="IVK82_002542"
     CDS             complement(7215..8837)
                     /locus_tag="IVK82_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393556.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     TFTLKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         2855 a         1701 c         1333 g         3079 t
ORIGIN      
        1 atattttcaa aagaaaaaag agaaggtgac aaaaattgtg tcaccctctc tttttttatt
       61 gttttttaaa gtgataggtt ggctcctcca ggttgggatc ataatcaaca agtaaatcat
      121 atcctttaaa gaaccattca tctgcttcct cgataaaaaa aagtgtccca tcaatttcca
      181 ctttctttac aggatgttct ggctgttcaa ctgacattcc aatcgaaaaa ccatcatgaa
      241 cctctgtttt gccgtagact tttccaaaaa aacgtattcc gtaagtttct ggcagatcaa
      301 tttctgagtc aaaccacttt tgtgcttttg gcgttaccgt taatttcatc ccaacaacct
      361 actttctctt ttctttctat tgattcatct tatcgtctag aatatccaca tcgcctgtct
      421 ctccaataac cagtagatta tcattttcac gaacatattc atctgctgca ggagaagcga
      481 taaccgtttg atctactctt cgtatggcga ctactgttaa gtcaaaacgt tgcctgaaat
      541 ttaattcagc taatgttttg ttgaaaaatt taggattgga gactcggatt tctgctaaag
      601 aatatttatc tgacaactct aggtaatcta aaatattctt tgacactaaa ttatgcgcaa
      661 tccttattcc catgtctcgc tcgggatgga cgacgcgatc agctccgatc ttttcaagta
      721 ctctggcatg atactcattt tgggcttttg ctaaaatatt tggtacgccc atttccttta
      781 ccatcagagt aactaagata ctggcttgaa tatcttctcc aatagcgaca attacatgat
      841 cgaaatttct tatgcctaat gatcgaagtg tcatttcatc ctgtgcattt gccacaacag
      901 catgagtggc tatattcata tattcattga cccgatcttc attactatca atagccagta
      961 cttcttgacc agcttcgatc agtgttcggc agatacttcc gccaaatcgt ccaagaccta
     1021 caattgcaaa attttgtttc accgttttcc tcctttagta tcttcttttc tcagtataac
     1081 aaagccttta caaaaacaaa agcaagcatc attattgtaa aaaagccaag aaaaataata
     1141 gttattttct cagcttttga ttatcctctt tatcatcgtt tttatttcac caaaccatgt
     1201 ttgaatgcat agatagcggc ttgtgtacgg tcttccactt ctagcttagc caatatattg
     1261 gaaacatgtg tttttactgt tttcagtgta ataaacaatt cgtccgctat ttcctgatta
     1321 ctttttcctt cagaaatcag cattaaaatt tcattttccc gattcgttag ttcttcatgt
     1381 aatacaggct catttcgccg actcattttt tccatcattt ttgttgtcac ttctggttct
     1441 aatactcgct cgccacgttg cgttgccctt atagcatcag caatttcatg tgcggtagag
     1501 gtcttcaaca aataccctgc tgcacctgct tcaatggcag gataaacttt ttcgtcatca
     1561 ataaaactgg ttacgataat gattttagct tctggccaat cttttaagat tgcttttgtt
     1621 gattcgatac catccatttc ttccatgact aagtccatta aaatgacgtc cggtcgaagt
     1681 gtcatcgcct tctcatagcc ttgtcgtcca ttttcagctt ctccaattac ttcaatgtct
     1741 tcttgaatag ataaataaga agagacgcct aagcgcacca tttcatggtc atctactaat
     1801 aaaactttta tcatccttat ctatgcctcc ttcatcaaag ggaccttgat ttctacactt
     1861 gtaccttgac ctttaaaact aatgattttt actgttccac caataccagc tactcgttct
     1921 ttgatattgt tcaatccata acttcccgtt ttcgtttcat tcatattaaa ccctgttccg
     1981 tcgtcaataa tacgtaacaa aagattattg tctattttgt gcaaatatac ctctaattca
     2041 ttggctttag catgtcttaa tgtatttgat aacaattctt gaacgattcg gaacaagtga
     2101 tcctcaatgg aactagatag ttttacatct tctacatccc atttcagcga aatctggatt
     2161 ttattttgca attccttcaa taactgctcg ataccttgct ttaaactttt ctcttctaaa
     2221 ttgactggac gtagatgaag aagcagcgca cgcatctcag actgggaagc gttgatgatc
     2281 tctgctacca ttttcaactg cttgcgaaac atttctggtg tctcgctttt ttctgcttgc
     2341 tccgtcaatg ccgacatcat catcattgct gcaaacaatt gttgcgaaac tgaatcatgc
     2401 agctcacggg ctaatcgatg tctttctaat tctaaaattt cttctttggt ttgtccatct
     2461 acatattggg gacggctcgt tacaatttgt aattcactag atacttcaat cattttttct
     2521 tttatcttag tgatttcctt atcaatatct ttgatgtata aatcgtccga tgcacttggg
     2581 atcggaagaa gcaacaattt ggattcataa ttgccagaag aaagtgcacg cagcttttct
     2641 tcgattttcc cgtattttgt cttttgaata agtgataata atagaaagac aattaatcct
     2701 acacctaagg aaatggctaa caaatggaag ataagcggaa catataataa acgtgcacgc
     2761 agtaattctc cccaccaatg actttgattg ctggcataaa aataagtgaa aagtgaaaat
     2821 agaataataa ggaaagccgc aatccccgaa taaacagcta gcatcgctct ggatattttt
     2881 cccatcatat tcgtatcacc tcgacatctc caatcaaggt attagtaata atcttcaatc
     2941 tacgagggtt ttcatcataa tcactgctgt agattttgat ttgttcattt ttcaaagaaa
     3001 attgctcttc ttcaaacaag acatttccta ctaatgtcgc atgctctaat cggatggcta
     3061 cgcccaaagg tactaagatc cgtgttctgc ctatcccttt tcttacaatc acaatattat
     3121 catcttttgg caaaagcgta ttgcccaaat ctatgatagt atcaccagaa atgacggcga
     3181 tgttgatatc gtcccattca tagacgtcgt ttccaatacg ttggttaccg aataattgct
     3241 gtcttttccg ctcattatta tgtgttttta attgctctgt ttggatcatc atgatctgtt
     3301 tctttcgcca aaaagcattt ttggttaaat ctatccctga aatctctaca cctttcagac
     3361 caataaataa aacggcaaat acagccatca tccataaagc aggactgttg actaagctaa
     3421 aaaaaatgac caaacttcct aatatcaatt gaaaattttg aaatttagta cgaggatgtt
     3481 tgcgcatagc tagatatata ttgaaaatac cgaatatcac taaaagcaaa agtccagtat
     3541 tgtttacgat ttgccaaaca gcaaataaca gcagtagtgc ttcaactacc acaaaaaaac
     3601 gccaagaact attcatcgct ctttctccct cctgacacat tctcatttca tcatacctat
     3661 ttttttaact gttggctata ggactttaga tgtaagatcg tttgttgaaa tctaaaatac
     3721 acaaagagaa aaagatctag agtgtatcat cagtaagatg aactctctag atctttcagt
     3781 cagactacgc ttgttctact ttgacgattt taactaacat atcgccacct ggagtagcaa
     3841 tagttacttc atcattttta cgtttgccaa tcaatgcttg cgcaatagga gaatcattgg
     3901 aaattttgcc agaaaacgga tctgcttcag cgcttccgac aatcgtgtac tcttcttctt
     3961 ctccatctgg tagttcgatg aatgtaactg ttttaccaat tgatacctca tctgcatcta
     4021 caccgtcgtt atcgatgatt tgagcaaaac gaatcatatt ttcaattgtt gtgattcttc
     4081 cttcaacaaa tgcttgttca tctttcgctg attcatattc agagttttcg gaaagatctc
     4141 cgaaaccgcg ggcaattttg attcgttcaa tgatttctcc gcgtttgact gtttttagtt
     4201 cttctaattc tttttctaat ttttctttac cttctagagt cattggataa actttctcta
     4261 ccatgtgtaa cgtctccttt tttcgataaa ataccgcaaa aggtaatctt ttgaggaata
     4321 tagtccaaaa aagattacca cgcttttctt caaatgtaaa tgtttttcta aaaatgatca
     4381 atgtctagag cctgtttttc taagaattat ttacatattt ttccactaat tccatatgtt
     4441 cttcataagt tttagcgaaa taaacatttc cagtgctcgt atctgctacg aaataataat
     4501 agtcattttc tgctggttct aatactgcct tgattgcctc ttcgcttgga ttgtcaaaag
     4561 gtccaggtcc atagccggta ttggtataca aattatatgg tgaatctaca gctgtatctt
     4621 catacgtcac taattctttg tgttcaccta atgcgtataa aacagagata tctgactgaa
     4681 gtggcatatt tgctttgatt cggttaaaga agacttgtgc aatgttttta cgatcatttt
     4741 cttttacgcc ttctttttca actagagaag ctagagttaa aacttcttgt acagtcaaat
     4801 ttttctgctt gatagctgaa taatagtttt gcatgaccgt attcgtcttg ttcaccattt
     4861 caatcactag atccttaagc gaagtactcg tgtaataatc atatgtggct gggaataagt
     4921 aaccttccaa acggtatttt gtgttctcag ctttagatgc actttccaac agttctggga
     4981 atttttggtg caactcgtta aagaaagtct catcattcac taaatctaaa aattctttct
     5041 tgtctttacc agttaccttc gctacccgtt cagcaatctg atcgatatcg tacccttctg
     5101 gaatggcaat tttcccatct gcaacttttg ttggctcaga agtaccgcct tcttgcaact
     5161 gtttgccaat ctcgtcaaga gtcatattag gcgctaactg ataatagcca gcttgaaagc
     5221 cagttaaatt tttaaatttt gtataataat tgaaaacaat cccgctttta ataatattgt
     5281 ccttctccaa aatctcacca atctgcttgt tagaagagcc actcgggatc tcgacttgga
     5341 ctaattggtc atctgattta tctaaaggct tcaacccgct atctacataa cgatacacgg
     5401 taaatccaag aaatccgcca ataatcaata atgtaagagc aatcactaaa atgatcttcc
     5461 tcacaatctt atcttctttt ttacgcagat ttctccgctt gtcgattata tctgctgtag
     5521 ctgccttact ggcttctttt ttatctttac ttgggtgttg tttgtttcct gacaaaacaa
     5581 ctcctcctta ctatcaagtt cgtaacttta ttcattatac atgatatttg aattggcgtg
     5641 aatgatttta tttaaatatt ctatcaaaaa tgtaagtagt aaatccatta ctataagatt
     5701 aaaataaatg ttatcaagat acaagctatt ttagctttat tctttctgta aaaaagaaag
     5761 gagactaaat aaactattat gaaaaaagga aaacatgcta aaaaaagaaa atattcattt
     5821 ttaatgatcg ttgtcgtgtt gtttagcctt agtgcgttct ctacaggaac tattcttgga
     5881 tggcaatttt acaataatga acactctcag atagttgccc atgctcaatc agctcaaaaa
     5941 aagttgatac tgactaccaa tgaacaacag ctcgttgaaa aaatcgatca actattaaga
     6001 aaaaatgatt atattggctc aatttacgta aagaaaaata atcgcatcat catggaaaaa
     6061 ggctatggct atgcgaataa actcacgcaa caaccaaatg atccttctct ttattatcag
     6121 attggttcgg ttcaaaaagc attgactact cttttgattt taaaacaagt cgagcgtggg
     6181 cacctttctt tagatactaa gctggctcaa ttctatccac aaattgctgg cagccagcaa
     6241 attactatcc gtgacttgct gtatatgcgc tcaggcttgc accgaactgc ttctccaacc
     6301 ataccgatga ctgatgaaga agtcgttcaa tttgcgattc agcacttaaa atttacagat
     6361 tatcagacct ctcattatga ttcattgaac tttaccatat tgactggaat tttgatcaaa
     6421 ttaacaaatc aaccgtatga aaaattattg accgatgata tcatcgaacc tttaggatta
     6481 cagcatacaa aattttatga ccaagtcaaa aattcatcca atcatgcact atcctatcaa
     6541 atgtctaaag aggatgatta ctttaaacca ctgacagaat ctgaaacaga gatccataac
     6601 gaattaggga ctggtaatat cagtatgtcc gtctatgatt taaatacgct gtttacacaa
     6661 gttttatctg gaaaactagt ttccaaagac ttactcttct cattatggcg tgaaaatgcg
     6721 aatggacatc catatagtgg tggggtatat agcggagata attatatcct gtcgcaagga
     6781 aatatcgact cttttgatac atctgctata tttagcaaag attcacagga tgctatcatc
     6841 atggaaagca atataaaagc tgacaaaact atcaaactgc ctactgctga tctaagaaac
     6901 caaatctatg acctgatgga aggaactact aagttggcaa atagctgata tgatgaaaag
     6961 atagcaaata ctacttcttt gaatagcatt tgcttaaaaa aagtactcga ctaaacgtag
     7021 gaaagagtgt gggacaaatc taaaaatcaa tttttgctac agactttaat tccaaataaa
     7081 cagaacagaa agaggtgttc ttctttctgt tctgtccaaa ttattctaag atttctcttt
     7141 accaacatta tttgacaaag ggcctattaa atctaaaata gtatctttag attgctaaaa
     7201 tatgtgctag caaatcattc atggcttgtt cttattttta attttatgca ataaaaaaga
     7261 agaacaatta gcgtgattat agaaataaat aatgagattt tagaaaaaaa tgtgtatgaa
     7321 tacctgattg ttactttatt tattccactt tcacttttta ctgtaacaaa aggacctcct
     7381 tgttttagta ctgtagcatt ttttgaatta acagtaaccg ttaatccttt ataccggaat
     7441 acaggtaaaa ctattgtttt gccttttgaa gatttaagcg taaatgtaat ttcatcttta
     7501 tttggagaaa agctactata aataacttga ccatcttctg taacttgatg gtttttcaaa
     7561 agcaattgat cttttttata gcctataggt acataatcaa aattctcaaa ttcttcaagt
     7621 ttattttcca tttgttcatt agaataagtt ccttgaaaat tcatttcttt caccatgtta
     7681 actactgatg aataatttaa aattgaaata gctaatatta ataatagtat aaaatttctt
     7741 ctccttgtat aagaaaattt ttccaactgt ggagaaataa atagagatac tgaaaaagat
     7801 atgaacaatg ttgaaaatat cataaaacgc caaggagctt gaataatatt taaaggagta
     7861 ttttgtaagg caaaccatgg aaatattttt gtacaaagta gaatagatat tatacccatt
     7921 atataagtga ttttagataa gattcctaat tttttaaagt taaatgttag tataattaaa
     7981 caaataagca aagttaagcc aataccaaat gatagtaatt ctgaatctat acttccaatc
     8041 aaaaactctt tacccagata aattgctctt ttttgtaata tatccacata aggtctattc
     8101 agatcttgat acaatgtctg ctcaatcata ggacctataa acgctagtga aagaagtaaa
     8161 gtaactatag cagcttttat taatttaata attaagaaga tttctatttt ctttttccga
     8221 taaaattgaa taattaaagc aattataatc attaccatag tcatagctac agaaagaaca
     8281 tgagtgtaaa ttaataaagt catgctaatc gccaacataa cccaagattc tttttttgag
     8341 tcgtaaagct ttataaaccc gagtaaaata agaggaagta ttgacattgc gagtaattcc
     8401 cccattgccc ctcgaagaaa tatttctaca ctcctaaaag aagatgttgt atatataata
     8461 gcgaaaaagg agctagtaac atgttttttg gtaatttcat acattacata atgacatata
     8521 aatagagtaa tgagcgtaac aatataaaga aaaaggttat agcctattgc taaattagta
     8581 aacttgatca ataaaaataa aggatataga gttaaccatg ggtaaaaatt atttacttgc
     8641 atgccatatg atctaaacgt ttcaaaatta ataggggagc taaaaataga ttctaaactt
     8701 aacgcacgat taatgtgaaa tgccgtatca taaaagccaa atatttccat gtttttaaat
     8761 gctggaaata aatataataa actaacaaat gcataaacta taaatatttt tacagaaaaa
     8821 atttttttca aattcatata ccacctcatg tttttttata ttacatatag tatcatttat
     8881 ttttttaaaa cactagatta gtcactatat aaaatgtgat taattttatt attaataaaa
     8941 tattttttat attacaaaag tagtgttt
//
LOCUS       DACCCS010000090         8724 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.094, whole genome shotgun sequence.
ACCESSION   DACCCS010000090 DACCCS010000000
VERSION     DACCCS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.094"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            151..567
                     /locus_tag="IVK82_002543"
     CDS             151..567
                     /locus_tag="IVK82_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393557.1"
                     /translation="MQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPE
                     IHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITEDQLSKYNLLEYD
                     LIVTNQPILNAHVPHILIDDSVSFANEEELIRLFEL"
     gene            complement(640..1287)
                     /locus_tag="IVK82_002544"
     CDS             complement(640..1287)
                     /locus_tag="IVK82_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6393558.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(1295..1981)
                     /locus_tag="IVK82_002545"
     CDS             complement(1295..1981)
                     /locus_tag="IVK82_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6393559.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(1987..3060)
                     /locus_tag="IVK82_002546"
     CDS             complement(1987..3060)
                     /locus_tag="IVK82_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP6393560.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            3282..3956
                     /locus_tag="IVK82_002547"
     CDS             3282..3956
                     /locus_tag="IVK82_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6393561.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            3961..5301
                     /locus_tag="IVK82_002548"
     CDS             3961..5301
                     /locus_tag="IVK82_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6393562.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(5450..6436)
                     /locus_tag="IVK82_002549"
     CDS             complement(5450..6436)
                     /locus_tag="IVK82_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP6393563.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            7160..8191
                     /locus_tag="IVK82_002550"
     CDS             7160..8191
                     /locus_tag="IVK82_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6393564.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            8300..>8724
                     /locus_tag="IVK82_002551"
     CDS             8300..>8724
                     /locus_tag="IVK82_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP6393565.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT         2766 a         1616 c         1483 g         2859 t
ORIGIN      
        1 atgtcctact gttcgttttc atccgctgat tgcaaagaaa aaaacgaaga atatgttgtt
       61 caattattaa cgcaaattta ttttttctcc cgtattttcc catacgatac gactcatttg
      121 acgatccgtg caacttactt tgcacagcag atgcaacacc gccagatacg tctatatgct
      181 ggatttcgtg cagaacttca attttattcc actctactga atcagaatct gatttattat
      241 ttatctagct atatcttttg gatgcttatc ggtaatcctg aaattcacca ttatacttct
      301 gataaaaaaa tactgattat cagcgactta agtcttcgac atagtcaata tattgaagaa
      361 tatatcagtg atattcttgc tgtgcataaa attcactctg agacaactgc tattactgaa
      421 gatcagctgt cgaaatacaa tcttctcgag tatgatctga tcgttacgaa tcaacccatt
      481 ttgaatgccc atgttcctca tatattgatt gatgatagtg tgtcattcgc aaatgaagaa
      541 gaactcatcc gcttattcga gttatgaata atcaaaaaac agctatgtat aaaccaccct
      601 gataaaaatt tcaggacaaa tacacatagc tgtttctttt tattgtattc cttcagcaat
      661 ccaacgagat ttatagcgaa agtcttttaa caactcttct tttgtccaat tatttgcaat
      721 gagatccgtc aaagtgttca ttacgagggt catggaaaag tataggaaac taactgcttc
      781 ttccgacgat tgaatggaga aatgattgat tcttaataat tccagccatt tttcgttcat
      841 tggagaatca gccgatagtt ctctacgttt ggttgtctgc tgatcatttc gctgggttaa
      901 caagtataac ctttgccaat agatactttc gtgatctaag tcgcagcacc actctaaata
      961 tttttcgatc cccaaaaaga aatgttgttt gttttcgcta atgaatttta agatatcttg
     1021 atggactatg tgagaactgc ttttgatcgt atacaaataa gcatcttcca aatcttgaaa
     1081 atatttataa aaagcaccgc gcgacatccc catcttgtca acgatttctg agactttgac
     1141 ttggctgatg tgctgtgagc aaaatatctc cagtaatagg tcatcgattc tctgtttctt
     1201 tgcgttcgat aaattgaaaa aagtttcttt tggcatccac atctctcctt tatctctgat
     1261 tatacgtgga tagcccttca ttgtcatgaa ggattcattc cttctttttc tcttcttctg
     1321 tcaagtaccc atcttccatt cgataaacgc gatcactcca ttgaatcata cggacatcat
     1381 gagtaaccat aatagtcgct ttttgtttct catgagcttc tttaactagt aatttgacga
     1441 cttcatatgc atgatctgta tccaaactag ctgttggttc gtctgctaag atcaagcttg
     1501 gctcattata taaagcgcga gcaattgcca ctctctgtct ttctccacca gaaaggtctt
     1561 taggataact atttttaagt gactggatat ctaaagattg cagcaaggaa tcgatttttg
     1621 ttgaatcttc tttttttgaa gtaacttggt caactagttc aaattgttcc tttaccgtta
     1681 aaaaaggaat caaattagag ctttggagaa taaatcctat ttctttaaag cgtaaatctg
     1741 ctcgtttttt ttctggaagc tcagttagtg agtgaccgtt gatcactatt tctccggaag
     1801 tcggtgtttg caatcctcct gaaatggtaa ggaaagtact tttacctgaa ccagaaggac
     1861 caatgatgct gacgaattct ccttccttta cataaaaatc aattgctttc aaagctgaga
     1921 cctttgtatg accagaacca aatgttttgg taactttttt catcgttaat atttttttca
     1981 tttttattaa cctcctattg ctgtaattgg gtctactttg gttatcgtac gtatcgaaaa
     2041 caatccgcct agcattgcga caatgatcaa aatcccacta tatagcaacc attgtgacca
     2101 aaaaacagca aaaggcatcg cgcttggtaa aatcagacta gtcaaataag caaacaaaag
     2161 agcaagcaga acgcctacca ccccaacaat aaaggactgt gctactaaag atttagcgat
     2221 gaagaaattt ttgatccctt gtgctttcat tactccaaaa atagcagtct tctgaagtgt
     2281 catcacgtac atgaaaatac cgaccacggc agcaaccact aagaacaaaa agtatatcat
     2341 cgcatttaag gtcatatttt gggcagaata gccaggaata ctttcaatga atgtcccaat
     2401 agttaataac tgtagaccct tcgatgcttt ttctttcgaa attttagctg aatcctttat
     2461 tacgatccca ttgatcggct gttcttcttc agaagcaaac ggctgttgtc catacttcaa
     2521 agcagtccac gtatctaggg acgcatagat gacgggagaa actgtgtaat aagtttcagg
     2581 aaagataccg acaatcgtca aaggatcgtc gtaactgcca attttgatcg tatctccaat
     2641 tttatagcct gaatcagcca gattttgaga gatgatgatt tcattctttg ttttaaattc
     2701 atgccctttc gtgagttttg gtaataaaaa tgcttctttc gttgtgccga aaactgtaac
     2761 attttctttg ttttttcctt tgattgctcc gctgtacaat ccgattggtg ctttctctcc
     2821 tccttcaatg tatttcagat ctcctcttgt taattgtgaa gctgcaaata cttgatttgc
     2881 atcctccgac aaaacaatct cttgtgcgtc ccaatcatcg attgcttttt taaactcttc
     2941 tgaaagtcca ttcgctagtc ctgacaacat gaatacgaca tacgcaatca agaacataat
     3001 accgacgatc aagccatatc gtaatttaga atagcgaatt tctttgatag ctagaaacat
     3061 ttgtttttcc tccctatgat agattgatag tgacaaaatg tcacttgttg aatagtgtaa
     3121 ctctaaagtg acaatttgtc acttgtgttg tttttttctt atcaataaat tgtctaagac
     3181 atctttcgat aaaaccttaa catgagtcat aaaaaattta tttaagcctt atttatgctt
     3241 tccatgataa actaaagtga ccaaatcaat gaggtgggaa aatgattaaa ctattagtag
     3301 tagaagacga taaagtatta tccaataata taaaagaaat actcgctgca atcggcgaga
     3361 taacacaagt ttatgatggt agtgaaggac tgttcgagtt ggaaagtgga gtttatgatc
     3421 ttgcaatctt ggatttgatg atgccggaaa tgaatggata tgaagtgtta gcaaaaatac
     3481 ggaaagaaaa aatacagact ccagtattga ttttgactgc aaaagatagt ttggaagata
     3541 agattgaagg atttgaacga ggtgcagatg attatctaac aaaacctttc tatcgagaag
     3601 aactgatcat gcgcgtgaaa gcattattga aacgctcgct aggtctcttt gatgaaaatg
     3661 ttctgaagta taaggatatc agttgtgatt taaaaagcaa tgaagtcttg tataaagaac
     3721 aagttttgcc catacaagga aaagagtttg aattgctcgt ttatttttta caaaataagg
     3781 gaatcatttt gacaaaagag cagatatttg accggatctg gggatttgat tctgatacga
     3841 cgattacagt tattgaagtt tatatgagca acttacgaaa acatctgcga agtactggat
     3901 tggataaaga aatcaaaacc ttacgtaatg tcggttatat actgcaaagg gtgtgatgca
     3961 atgaatggag atattacacg aaagcaacgt agtcgctttt ttgcgacaaa tgtagcagca
     4021 tttgctttta tcttttttct tttgggattc attactttac agatattaaa ccaatctgca
     4081 tacagagaaa cagatctgtc attagagaaa ataaattttg attctcgaac agtacaatta
     4141 gagatacaaa gatatcagca aggcgatccc tttttaaacc cgactcaaac ggaacaaaac
     4201 agaacaaaca tggaacagcc attaatggaa ccatcaaatg accgttttaa cactcaagtg
     4261 atcttgtggt ctaaaaatgg tgagatattg aataaagcag ctattggtgg gcgtttcaac
     4321 caaatcaaag aattaaaaat ggatacgaaa aatctgaata ccatccaaaa tattgaatta
     4381 aatgaagaca gtggctcatc tgccctctct ttccgttcga ttacgaaaaa agcggagacg
     4441 aatcaagatg gcgttgccta catccaaatt ttagaaaata caaatcaaat caagaattca
     4501 ttagcgactt ttcgcacgat tatgattttg tgtatgattg ttttttggat tctctctatt
     4561 ggtatcagtt attacttgtc tagtctaagc atgcgcccta ttattctttc ttggagaaga
     4621 caaaaagaat ttgttgagaa tgcttctcat gaattgcgca caccgttaac gattattcaa
     4681 aatagtttgg aacatctttt taccaagcct aatcatacta ttattgaaga atccgaaagc
     4741 atcgctcaag cattgtcaga gacgaggcgc ctcactggat tgacttcgga tttgctgaca
     4801 atcgctcgga atgattctga tcagcagctg ctttcaaagc aaatgatcaa tacacaagaa
     4861 tatatcgaac aacttgttaa accatttcaa gagatggcca tcatagatgg aaaagagttt
     4921 attctccata acgatgcaac attagaagta ccaattgatg aaaaaaaaat ccatcaagtt
     4981 ttggttattt tgctggataa tgcattaaaa tatacgaatc cagaagataa gatcattttg
     5041 gattcatctg ttaaccataa agaatggctg attcacgtaa agaatactgg cccttctatc
     5101 agtgaagaag atcaagcaag gatttttgat cgattttatc gggaagatca atctcgggcg
     5161 aaagagactg gcggatacgg gcttggcttg gcaattgcaa aacaggtaat cgaacagcat
     5221 catggacaga tttctgtatc caattatctt ccacaaggag tcgattttaa agtccaattg
     5281 ccgttgaagc ctaaaaaata atgcttatcc ctaaaaaagg gctgtgacaa aaatcttgtc
     5341 acagcccttt tttgttgcga ataaacggtg ttccagaagt agcttcttcg gaaataagcc
     5401 gaaatttctt taagctgacc acttctgtca caacctcttt agcttacgct tacgattttt
     5461 tattgcggtc ttcaatgtgt acatcaccgc ttgtgtcaat atccaattct tctcggctaa
     5521 catcttctgt cactttttca acatcttcat gttcttcttt atgaatccca acttcttcac
     5581 gaataactgt atgtttgctt acatctactt gctcttcttt gattgggata gtaatcgtgt
     5641 catcttccaa tttttcgtct gttccctgtg aagattgatc tgttactggt ttgcgttcga
     5701 tgacgatttc ttcacgtttg accggtactt ctaccgtttc agtatcgttg accacatgtt
     5761 tgtggatgtc gacttctcca gttgtcacat catgtgtatt tacatctaat ttttcttctt
     5821 ttagttgaat tgtatcatca tctagatttg gtttgcgatc gatgtcttca gtcttgcttg
     5881 tacccattgt atcagtctcg ccaggtacgt ccatcggatc agttcctggt gtcatattca
     5941 atggatccgt tgtattttga aagcctgtcg tgccacctac gtccattgga tctgttccag
     6001 tgccagtttc taatccagtc cccattgtgc ttgctgggct aacagtatct tgtgtatcca
     6061 ttgtatcttc tgttgtttct ggctgacgat agccttttac tgtgatgaca aattttccat
     6121 cagaaatatc tcctctatat tgatataaag gatcattttc tggtgtagcc gtctcagaat
     6181 cataggtatc aaaagaaaaa gtatctttta ttttttccca taaagaacgg tcttcttcac
     6241 catccatccg attttcatct gtttcaacat ctataccaga caatccgtca aatcctagtc
     6301 tagatgctgc ttcatcagta gtataaataa cgatatcatc tcgttcatac ccttcgttac
     6361 gcaaccgttc aattactgat aatgcttctt ccctcgtact atagcttccg atgaccgtta
     6421 aatttttctc ttccatgtaa attcctccta gcaaatagat ttatgattgg tatattgtta
     6481 cttataaatt tattctatag gagacgattg ttttctacaa atatttagat tggaatttga
     6541 ttaaaacaat ttaattgaaa tttgaaaaca tttatgttaa tgaaagtcat attctaataa
     6601 aatgtcattt gtttctatat gaatttttat gaaaaatttc atataatttc cactcgttgt
     6661 gatggtttaa tttatccaat ccatcaataa gcaaacaact tatataaaaa aggattaatt
     6721 ctcaatataa ttgctcctac catagaataa atcttcatgt aaagtaatga agtaccaact
     6781 atttctaata cttctttaag aattatccca acaatatcgt tagacaaacc attattgaat
     6841 aaatggagag ccaatcgatt ctgaaaaaat gttttctaat ttttcggaaa taaaagaaag
     6901 agcctcctgc cattagaacg aaccaaatcc atttcattgc tcatgaagta accacatagc
     6961 taaaaagagg ataaatcaat taaaagatca aaagaatgac tgcactaata aaataactaa
     7021 aaatgtatct tcttacttca tatcttttag ctagtactta gttaaaaatt tgcttcctaa
     7081 gatcaagaca aactgtctat cttcatttga tgaaatgcgg taaaatatag aaaatgaaca
     7141 ctattggagg gaatttgtaa tggacagcaa aactagaaaa caactgcaag aaaagttaaa
     7201 gcaacgtaca cacttgctcc cttccgtctt tcaacagttt ttagaagaaa atcgttcttc
     7261 cctttcatta ggcagtcgct atgaatacgc aaaagatttt catctgttta ctgattattt
     7321 gagaaaagta ttttctgaag aagtggatga tcagctgatt atccatttag aaaaacaaga
     7381 ttatcagaat ttcttagcta aaatctatcg acatactcgt tcgttccaaa ctacagcgaa
     7441 ccaagaaacg gagcaattgt ttgaaaatag ctattacggg atttcaagga agcaatattc
     7501 attaaatcat tttttacgat ttttgtatca aaaaggactg attgaagaag agatttctct
     7561 tttgggtaca agtttaccgg aaacaaccaa agcagctccg cgatatatcg atgcagaact
     7621 tttcaaagat tttgagcatt tgtttatccc gattgaaggc tttcaaacaa gcagagaagc
     7681 tagctttgaa gaaaagaata gaccgaggaa ccttgcaatt actgccattc ttctgtattg
     7741 cggattaaag atccatgaag tagtagaact taaacgtaaa gatgtatctt tgaccaagca
     7801 gattcttaat ctgaatagaa aagaaaacag actgaataaa gtaccaatac caaaagaagc
     7861 gatgccattt ctggagaact attttaagtt aacagcacaa aatacggacg aaaattcttt
     7921 tgtttttcgt tcctcacatg atcagcaaat ttctcctcgg actattcgcc aaattttgag
     7981 caaaattaca gtatataaaa acgtatcgcc agaactatgc agaaaaacat atcgttatta
     8041 tgcagaacta tatttagatg atgctgacgc ggtaaattat ttatgtggta atcgatacct
     8101 ctcatcccat tcttttcaag atatctggga aaagatggtt gatttttctt atcacgaatt
     8161 agcggcattc gttcgggttt cttccatata aaacaaatgt atgatctttt tttcacaata
     8221 aactatgtta ttatataaag gattatgtta cgattatctt gaatttgtat attacttaac
     8281 aaaagataat ggagtgtgaa tgatggagac cataacgtat tcccctttga acttatttac
     8341 gaattttcaa gaagctgcag ctaacttttc cgagaccccg attatctttg atgagccact
     8401 ttcagctttc ccagaattag gacttgaaac aacttacaaa aaatgctgcg aaagtgtgtt
     8461 gaaacgggct tatcaattgg ctcatcttgg agtgaaatcc ggagacaaag ttattattta
     8521 taagagttcc gcttttgaca cataccttct agctgtttct gtgtcttacc ttggtgcagt
     8581 gcctgtcatg acttcttatc atctaccaac aacaacaatg gaagtattta tcgatcgttt
     8641 agaagaccct ttcattttat ttgatgatga gacaaaaaag cgagtgagag aaatttcaaa
     8701 tggaacaaaa tcaaaacaga ttcc
//
LOCUS       DACCCS010000091         8662 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.095, whole genome shotgun sequence.
ACCESSION   DACCCS010000091 DACCCS010000000
VERSION     DACCCS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.095"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="IVK82_002552"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="IVK82_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP6393566.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /locus_tag="IVK82_002553"
     CDS             complement(740..1462)
                     /locus_tag="IVK82_002553"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP6393567.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /locus_tag="IVK82_002554"
     CDS             complement(1598..2479)
                     /locus_tag="IVK82_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP6393568.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IVK82_002555"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IVK82_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP6393569.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="IVK82_002556"
     CDS             3719..4198
                     /locus_tag="IVK82_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP6393570.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="IVK82_002557"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="IVK82_002557"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP6393571.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..5627
                     /locus_tag="IVK82_002558"
     CDS             5499..5627
                     /locus_tag="IVK82_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP6393572.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5631..6176
                     /locus_tag="IVK82_002559"
     CDS             5631..6176
                     /locus_tag="IVK82_002559"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP6393573.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6630..8321)
                     /locus_tag="IVK82_002560"
     CDS             complement(6630..8321)
                     /locus_tag="IVK82_002560"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP6393574.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
BASE COUNT         2410 a         1772 c         1568 g         2912 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttgatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgcgcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccgcccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgcg tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattga
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaaatttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa cttctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcagc tccttcgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatag
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccgtctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaagagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgatttgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactgga
     5041 aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtgctac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa tggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaacc caactgactg acaaagaaat ccgctaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gaatggattt aatgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa
     5941 cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaagatc gtttaaaaca
     6181 gtctgttgct cttagattcc aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga
     6241 gttaacagaa tacattttgt acgctgacaa gaatccagaa tgacttttat acaaaataag
     6301 aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa aatagctcct
     6361 catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
     6421 cgaaaaaaaa aagggagctg tgacacgact tttgtcacag tccccttatt tgcaaacaaa
     6481 cagtgtttaa gagacaacct ctccactaaa cggtgttcat tcagctgact ctcggtattt
     6541 gctcaaaaaa cgataaaatt tgaaaagcta ttttctcttt tgtttctcta atactcggat
     6601 cagaacgctg atttcacatt ctctttttct tacatcttat ctggtgcatg aagtcctagc
     6661 aaacgaaggt cttctttcaa taatacagtc actgcataaa ctagagctaa gcgtgcttct
     6721 ttttgttcat catcagctaa aatcttcgta tgagcatagt atttgttgaa tgcttgtgct
     6781 aatttgattg catgtttcgc aatcactgac ggttcgtatt tttccccagc agacaagacc
     6841 gtttcagggt atttttgaac taatttaacc acttcccagc tggtatcgtc attcaatgca
     6901 tagtctgctg tttctgaagg gacaaatcct gctttttcca acaggctgac tgcacgagca
     6961 tgtgtatatt gcacataagg tccagtttct ccttcaaatc gaacaacttc ttccaggtta
     7021 aagtcaaatg tattcagtcg atcgtttttc aaatcgtgga aaatgactgc cccaacccca
     7081 acttgtttag ctacagcatc tttgttttcc aaatcagggt ttttctcgct gatttgttct
     7141 ttagcagatt ggattgcttc atttaatact tcttctaata agacgatctt tcctttacga
     7201 gttgataatt ttttaccacc ttgcgtgatc aaaccaaatg gaatatggtg catgtcgtca
     7261 gaccagtcaa agcccatttc cttcaagaca gctttcaact gtttaaaatg atagctttgt
     7321 tcattcccta ctacatatag agattgtttg aagtcgtaag tacgtttacg gtacaatgca
     7381 gcagctaagt cacgtgtaat gtatagtgtc gcaccgtctg acttcttaat caacgctgga
     7441 ttcaggtcat aagcagatag gtcaacgatc tctgcacctt tatcctcgtt cagcaaatgt
     7501 ttttcttcta aaagtttgac gatttcatcc atcttgtcat tgtagaaagc ttcaccgttc
     7561 aacgaatcga aacgtacttc cagcagatca tagattttat tgaattcttt catcgattca
     7621 tcacggaacc attgccataa ttgaatagcc tctttatcgc cttcttccag acgtttgaac
     7681 caagcacgag cttcttcatt taattctggt tctgtttcag ctacttcatg gaattggacg
     7741 tataaacgaa gcaattcatt gattggttct gctttaacag cttcttctgt accccatttt
     7801 ttgtaagcga cgatcagttt tccaaactgt gttccccagt cacctaaatg gttgatgcgg
     7861 attggctgat aaccgatttt ttccataatg aaaccgatcg aattaccgat aactgtcgaa
     7921 cgcaaatgtc ccattgagat tggtttggca atattaggag aagacatgtc gattggtact
     7981 gttccttgat tcccgatgtt gctatcccca taatgttctt tttctttcac tactgtttgc
     8041 aaaacttttt tgctgataag ttctttattc atgaaaaagt tcaagtaagg tccgacaact
     8101 tcgatttttt caaagttggc agaatcgatt ttttctgcta aatctgcagc gatttgctgt
     8161 ggcgcttttc ggtaaacttt tgctaatgaa aaggctggaa atgcaacatc tccgtgttca
     8221 gctgattttg gattttctag taattgctcg acttgttcca gtgttaaatc atctttgaca
     8281 gcgtcataga cagcttttgc gacaagatct ttattattca tctttattcc tcctaaagtg
     8341 ttcccttgat tgttaaacaa aaaaaatctc tagcgacggc atacttagta tgccatcact
     8401 agagacgaga tttcccgcgg taccactcca attgtcctaa aaaaggacca gctccatctg
     8461 atatcggtag acgaccgtct gtttaaaaca gaattctcca agtgcgcttc gttgtatctt
     8521 cttcattcgg cttccaccac ttccgaattc gctagccaag aatgatacaa gtactctctt
     8581 gttcaacaaa tcttagtctt attaaacttt cttgaattat atcagaaaaa aaaacgaaag
     8641 gctagtcgtt tcataaaaaa aa
//
LOCUS       DACCCS010000092         8555 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.097, whole genome shotgun sequence.
ACCESSION   DACCCS010000092 DACCCS010000000
VERSION     DACCCS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.097"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="IVK82_002561"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="IVK82_002561"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP6393575.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="IVK82_002562"
     CDS             complement(1098..2201)
                     /locus_tag="IVK82_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP6393576.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /locus_tag="IVK82_002563"
     CDS             complement(2291..3226)
                     /locus_tag="IVK82_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP6393577.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="IVK82_002564"
     CDS             3528..5462
                     /locus_tag="IVK82_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6393578.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="IVK82_002565"
     CDS             5919..6335
                     /locus_tag="IVK82_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP6393579.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="IVK82_002566"
     CDS             6349..6915
                     /locus_tag="IVK82_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP6393580.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="IVK82_002567"
     CDS             6938..7924
                     /locus_tag="IVK82_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP6393581.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8555)
                     /gene="scm"
                     /locus_tag="IVK82_002568"
     CDS             complement(8079..>8555)
                     /gene="scm"
                     /locus_tag="IVK82_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP6393582.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2515 a         1651 c         1738 g         2651 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaagggg tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc catcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgct
//
LOCUS       DACCCS010000093         8493 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.098, whole genome shotgun sequence.
ACCESSION   DACCCS010000093 DACCCS010000000
VERSION     DACCCS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.098"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..173)
                     /locus_tag="IVK82_002569"
     CDS             complement(<1..173)
                     /locus_tag="IVK82_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6393583.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IVK82_002570"
     CDS             complement(593..1714)
                     /locus_tag="IVK82_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP6393584.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /locus_tag="IVK82_002571"
     CDS             2042..3451
                     /locus_tag="IVK82_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP6393585.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IVK82_002572"
     CDS             3448..4176
                     /locus_tag="IVK82_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6393586.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IVK82_002573"
     CDS             complement(4304..5215)
                     /locus_tag="IVK82_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6393587.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IVK82_002574"
     CDS             complement(5232..6239)
                     /locus_tag="IVK82_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP6393588.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IVK82_002575"
     CDS             complement(6236..8365)
                     /locus_tag="IVK82_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6393589.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IVK82_002576"
     CDS             complement(8370..8477)
                     /locus_tag="IVK82_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393590.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2682 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DACCCS010000094         8479 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.099, whole genome shotgun sequence.
ACCESSION   DACCCS010000094 DACCCS010000000
VERSION     DACCCS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.099"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            1..382
                     /locus_tag="IVK82_002577"
                     /pseudo
     CDS             1..382
                     /locus_tag="IVK82_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(489..1520)
                     /locus_tag="IVK82_002578"
     CDS             complement(489..1520)
                     /locus_tag="IVK82_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6393591.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(1527..2357)
                     /locus_tag="IVK82_002579"
     CDS             complement(1527..2357)
                     /locus_tag="IVK82_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6393592.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(2354..3160)
                     /locus_tag="IVK82_002580"
     CDS             complement(2354..3160)
                     /locus_tag="IVK82_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6393593.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(3166..3990)
                     /locus_tag="IVK82_002581"
     CDS             complement(3166..3990)
                     /locus_tag="IVK82_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP6393594.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(3980..5080)
                     /locus_tag="IVK82_002582"
     CDS             complement(3980..5080)
                     /locus_tag="IVK82_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6393595.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            5258..6043
                     /locus_tag="IVK82_002583"
     CDS             5258..6043
                     /locus_tag="IVK82_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP6393596.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            6076..6459
                     /locus_tag="IVK82_002584"
     CDS             6076..6459
                     /locus_tag="IVK82_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP6393597.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            6535..6666
                     /locus_tag="IVK82_002585"
     CDS             6535..6666
                     /locus_tag="IVK82_002585"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6393598.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            6635..6871
                     /locus_tag="IVK82_002586"
     CDS             6635..6871
                     /locus_tag="IVK82_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6393599.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(6949..7920)
                     /locus_tag="IVK82_002587"
     CDS             complement(6949..7920)
                     /locus_tag="IVK82_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP6393600.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         2494 a         1969 c         1642 g         2374 t
ORIGIN      
        1 atgataaatg gacaaaatgg cgtaattttg gttgggccta tttaaccgaa agtgaagtcg
       61 tagaacgtct actttctact tctgatgaac tacgaatagc ttatgcttac tatcaagaaa
      121 ttcttcaagc tttttatgac aaagaagcgg acatcttctt tcagcttgta aaaacaatgc
      181 ccaagtcggt tccaagagaa ctgcatcata taaaaaaagc gtttataaac tatgaatccg
      241 gcattcgttt agccttagag ttgccttact caaatggcaa aatagaaaat ctgcataccc
      301 acatcaaagc attgaaacga atagcttacg ggttaggatg agaaccagaa ttttcttaat
      361 caatcatctg atcacttatt aaacataaaa aaacatctaa agcagaaaat caattctacc
      421 ttagatgttc ttatatcact ctatcagtcc tatttgacaa agagccagaa atgtttgccg
      481 actatacact agctttgttg tgcaaaatgc tgagtatcaa tttccacagg gattgctaga
      541 atatctttca gctgaatgta taaggcatcg ctaattttct cttcccgatc gttcaaccga
      601 tccactaaaa tttctttgcc ctccagcgtt tgaaaaacat accgttgaat gttgcctaaa
      661 ataaatgatt ccttccatag aaccggaatc ttaaccccct gaggaaccgg ttcaaattga
      721 atcagctccg gtcttaaata ataaatgcag cgattgccct tagcggaaaa ctcgtgggta
      781 tactgattca gctcatacca ttcgaaacga ttatagttgc cgataaattc cgcaacaaat
      841 cggctctgag gattctgata caattccttc gaggtggaaa cctgagccac ccgcccagct
      901 tccatcacaa aaatccgatc actgattcgc atcgcctctt cttggtcatg agtcacaaaa
      961 atcatcgtca tccctagcga ttgctggata gcgcgcaaat cccgctgcag ctgttttcta
     1021 attcgggcat ccaaagcact taatggctca tccagcagca ggactttcgg ttcagtgacc
     1081 aatgagcgag ccagtgccac ccgttgtcgt tgaccgccag aaagaaactg gacattttta
     1141 tctgcctgct ccgtcatatc caccaatgat agcatctcaa aaaccttttc ctgaatcacc
     1201 tcaggactca gcttctttac cttcaaacca aaggctacat tttcaaaaac agtcatagtt
     1261 ggaaacaatg catactgttg aaaaacaaag ccgatattgc gatccttcgt aggcttatcg
     1321 gcaatattct cattgtccag cacaatctca ccgctctttg gttgttccaa tcccgtgata
     1381 cagcgcagca acgtagactt tccgcacccg ctgggaccta gtaaagtaac cagctcaccc
     1441 ttttcaatct gaatttcatc aatagctaaa atctgttttt catcaaaaaa cagattgaga
     1501 tcatgtattt ctaaaaacat tttccgtcac aactccttta agggcttctt tttcgactgc
     1561 ttctcttccc tcgtctgctt aagggacagc tgttgctttt gtcgataagt cagcataaag
     1621 gtagttaaag caattcccac cataaacagc aaatacaaaa ccatcacagc gctagccaaa
     1681 tggccgtttt cgttcatccg ccgcagcata tagatgcgta ccgtctgaaa tcgaccgcca
     1741 atcaacaggt tcgtcagcat gtactcccca aaaaccgaag aaaaaatcat cagagaaact
     1801 agggtcacac cgatacggat gtttggaaac agcacctgga taataatcgt agacatcggg
     1861 gcgcccaagg tttctgctgc atccactaat tccttcagat taattaagcg catctggttg
     1921 ttaagactaa gatagacaat cggcaacgcc gtaataaaca atgcacctac caacactaca
     1981 aacattggga taccagtttc agcatacgtt cgaagcaaag ccgttaccaa aatcactcct
     2041 ggtaaagcat acggcagcag gagcagcttt tccagccaac gattcaaacg agggaaatac
     2101 aaatgaatcc aaataatggt gggaatcatc aacaacagaa tcgtcaccaa gaccacacct
     2161 gctaataaaa gtgagcgacc caccgcagca ataaattccc tgtccgtcag cagctgttgg
     2221 aaccattgca aggtaaaatc actaggtaaa agcgattttg accatttagt ggagctggca
     2281 tacaaaatcg ttccaataat cggcagcaag taatacagga caaagcagaa caaaatcggt
     2341 tttattaact ttttcacgtc tgcaccacct tgcctttctt ttgaataatc aacattgccg
     2401 ttaccagcag gacacctaaa gcgactgcca acgcactgcc taaattaggc ttggcgaaca
     2461 catctcccga cactaaagcc gcaattcgaa tcgttaaaag atttacatta ctgctggtca
     2521 gggcatacgc tgtttcatag gtccccatgc cattagcaaa taagatgaca aaggtgctgg
     2581 caatcgatgg tcgcaaaaaa ggcaaagcca ccttgaacca agcatacgct gatgaagcgc
     2641 ccagcaattg tgcggactcc agccattcct ttttcacttc cttcatgctg ggatacaaaa
     2701 acaagacccc taacggaatt tggaaatagg tatacgtgat ggtcaatccc caccaagaat
     2761 acaaattgaa gtccgctaga aatggcaaaa tcattttcag cacacctaca ttccccaaca
     2821 aaataataaa agagaatgcc agcggaatcc cagcaaaatt agctgccaaa ttggaaacaa
     2881 aggtgatttt ctcctgcaat ttagtcgata gattcattaa gcataaacat aaaataaacg
     2941 tcccgattaa cccaatcaca ctggagctga cagcaatcag tacgctgtta acaaaggcct
     3001 gtgtgtaata tttgttgctg aaaatatcac tgtaattggc caatgtccac tggttagtta
     3061 aatcactgtg caagctgcac agaaccataa aaagcagcgg cagcagtaaa aatgcacaga
     3121 agataaagga ggcaggaacc agaaccaagc gttttttcat ctctttcatc ccctcatcaa
     3181 ttcttgaatg tcctgcattt tctctgacgg tgcaattcct aataaccagc aaaacaatgg
     3241 agccgccagc agctgtggta cagtttgttc cttcagcttt tggcgtttat ttgaaatcaa
     3301 ataaaaagga acttcacgat gtgcagccaa gctgccgcca tgcagtccgt gcgcatccat
     3361 cccgtgatcc gcagtcacca cgacttgata acctttttgc agccattcag gaaggtaaat
     3421 agccaataat ctgtccgcct gattaaccgc tgctgcatat tcatgagaat cggcggtaaa
     3481 tttgtgaccg acatcatcaa tattcatcga atgtacgacc aaaaagtctg gctgatactg
     3541 attcactaaa taatgggcat ccgcaaataa atgcgaatca gggtaagtgt cctcactata
     3601 aaagatgcca tgctgaatca gtcgctcctc ctgatgctgg atgcgatcaa ccaaacgatt
     3661 atagggggct tgattgtaca actcgctgta ccagtggtaa gcggcggctc ctgtttttcc
     3721 gccagcttcc ttaactaatt caaacagcga ccactctttg gatcgctgag catggtaatt
     3781 actatagatg ccattttcat aggctggaac gccggtcatc agtacttcgt ataacggacg
     3841 tgaattgctg ggcagctcac tttttacctt caataagctt gcttgctggt gctcaactaa
     3901 atgattcatg aacccaagct gggcgacggc tgtgtcgtag cgacagccgt ctagtataac
     3961 tacaatcagt ttgtcaagtc tactttgcat aggtcagtac ctccgcctgc caaagctctg
     4021 gaagctctac agaggtcttg ttccaagctt cattgtcttc aactggcacc gccgcttcat
     4081 aagcatcctc cggcagtaat ttttcagcta cttctttagg cagctctacc ttgcgaatag
     4141 gtcgcgcata gccctttgcc aaattgattt gcccttcatc agataaaata tattcccgtg
     4201 ctagctttgc cgcattggga tgaggggcat ttttattgat taaggttgta tacccgctaa
     4261 tgatggagcc gtctgttggc acagttacct caaaacgctc ggagtcaatt tggtcacgat
     4321 aatttagtcc attgaagtcc caaacaactg ccacatcgat ttcacctttt tcaagatttg
     4381 ctacgctggc atcaacggtg gacaaacggc ctttttctgc catttttttg tagtaattca
     4441 gacctggctg aatatccttt tcgtcacctc cgttggcaat cgccgcagcc aatacagcaa
     4501 attgtgcttg gttggcaatc gtcacatccc ccacgctaac ttgatagtca ccctcttgca
     4561 gctccttcca gctagtcggt gcttgcttca cattctcttt atcggtgata aaggaaatgg
     4621 ttcccgtata gcttaacaac caatccccat catcatcctt tgcccaatcc ggaatttcct
     4681 cccaatagtt ggtcttataa ggtaaggtta actctttgct ttcagccaac ggaccgaagc
     4741 tgataccgac atcaccaata tcggcggttc cattttttcc ttctgattca aacttagcca
     4801 attcttctgc actggacata tccgtatcca cgtgacttaa gccgtattct ttttcaaggt
     4861 cgttccaagt gcctacccaa tttgcccaag tatccggcat tcccaagctg gcaatctctc
     4921 cttcttcctt ggctttttct ataatcgtat ctaaagagtc gtctgccgca gccgattgct
     4981 tcgttgcttc acctgagacg cagcctgtca gcaggatact tactgttgct gcacaaaata
     5041 aaccgatagt catgttacga attttttcat tcattttcat tcattcttct cccctttcga
     5101 taattccatt ctaacaaagc tttgtaatga gagaatcagt ttcttgaaaa ggtttcatga
     5161 acaatgtaaa ggttgtatat ggtttgtaaa tttcgacgaa ttcccgttaa ccttctaaag
     5221 cttttgcggt atgatagtag aaaaaaggag gctccctatg cagtattatg atcagcatct
     5281 ccacacgtat ttttcctttg attcagagga aaaatttgaa aactatttag cctatcagcc
     5341 ggagtttttc gtttctacgg atcactttga tttaaacaat ccatctaccg gaaaagatga
     5401 cattccggat tatccagcct atataaacaa actggactct ttaaaagaaa cctattccac
     5461 caaatttctg cgaggtatcg agattggcgt tgttccagga caagaagagc ggatcgtgga
     5521 ttacctcgct cagcatcctt atgatttaaa gctggtaagt attcatcaaa atggccgctt
     5581 tgattatatg gacgatgtgg ttttaaccaa agatcccatt caaactacca aggaatattt
     5641 tgatcagatg ttgcaggtct tgacggattt tccagaagga gatattttga ctcactttga
     5701 ttatggatta cgccgcttca ccttcacagc ggaagaattc gcggagcatt ttgaggctca
     5761 gctgctaacg attttcaaaa aggttgttga acgagaattg gcgatggagt taaatgccaa
     5821 gagcttcttg aaatacggca acgaagacct ttaccgttat gccgttcctt tgtatcaaag
     5881 tgtcggcgga aagctcttta ccttaggctc ggatgctcat gttgccgaag attatcagct
     5941 gggctttaaa aaaatgagtc agctgttggc agactgtcag gtagaaaagt tgctagtcat
     6001 tcaaggaaaa gaacgccgcc tgacgccgct gccgattttg tgattcctat ataatagaag
     6061 aaagtggtga aaaaaatgag caacctgtgg gactacaatc aagaggcacc catccattat
     6121 ttaattgcgc gacattggga tgcgttgaaa attgaggcgg tttgccgatc cttgttagca
     6181 gccgttccaa aacaacaact ggaaaatttt ttggtggctg acagcttgca gcgggaaaaa
     6241 gttcaggctt attttgcagc ctttaaagat cagcctttgg aatatttgca tgcgcagttt
     6301 catctttttt atcaagtagc cgccccagat gactacaacg atttacgagg acagctgcaa
     6361 ttgacctttc aagcggatga aaccgcctat accgttttat tgggaatggc tcgccttggt
     6421 gatcaggcga aggtcgagtg gcggattttc gatatttgat ggcaggatca aggcgtttca
     6481 gttgcaaacc ttcctctctt tcaattagaa aaaccgcagc aggaagaagt ttgtgtgctg
     6541 gtcaccgatg ctggtgaaat tcgtctccgc ttatttcctg accaagctcc cctggctgta
     6601 gccaactggc gctccctagc tgagcaaggc gtttatgaca acacaccttt cggctcgtgt
     6661 cattaaggaa tttgttattc aaggtggcgc tttggatggt tctggtgatg aagctgtttc
     6721 cagctatgag ggctttttcg ctgatgaagt gcatcgcgga ctttatcatt ttaatggcgc
     6781 cttggcctta ggggaccacg gcccccacac caacggcaac cagtttttta ttgtgcaaaa
     6841 cacaaaggcg caggctgacc tgctaatgta aagcaagcat acttggagct tggaggacta
     6901 ccggagctgg atggtcgtta tactgttttt ggacaggtat accaagggtt agaaatcgta
     6961 gcagcgatcg cagctcagcc gacaaacagt gaagatgtac cgattgagcc agtgttcatt
     7021 cgacaaatta acttcgaaaa aacatcgtaa aaagacactc actgacttca gcaaaagctc
     7081 tattcttgag ctggctgcag ctagtgagtg tctttcattt atccataaga taaagcttgg
     7141 attcaataaa taaataaatg ttttggttaa agctgtgacc agcaaatgag gaaccgtgcg
     7201 agccagttga tctgccaatg caaacatttc tttaggtgaa ggcgctggcg tgtctgccaa
     7261 cggatactcc agtcccaatg cttggtaacc gtgcatacca tagcagtggt acggtagtaa
     7321 ttctaacggt acgaaccgat caagatctgc cacaaaacaa gcaatcgtca gcagctcggt
     7381 caggttcata ttgatacctg gaaccacagg aactcgaatt cgcacttcgc agttaggata
     7441 ctcaattaaa aagcgacgaa tattcttcaa aatttgttgg ttatctaagc cggtccattg
     7501 tttgtggagc ttgctggtaa acatttttac atccacaaac aatgtatcca catagggagc
     7561 taccttttgg atttcattat aagcaccaca aaagctggtt tccagtacgg tattgattcc
     7621 aatgtatttc gatttttgca aaatttcttt agcaaaatcc gcctgaacca gtgcctcgcc
     7681 tccgctaatg gtcactccgc catccgagtg aaagtaaaaa acagcatcct tctcaatctc
     7741 atccattacc tcttcagcgg tcatggtctg gccaaatcct ttttcaatct gcttgctttg
     7801 tgattcggga atcgaacacc aggcacagcg taatgggcag ccctttaaat aaacaacggt
     7861 ccgtaaacca gcaccatcat gtagagaacc ctgttctatt cttaatactg ttccttccat
     7921 atagattttt gaattacaca ctagtttaaa ctaaaacaca caaggaaaaa cttaccattt
     7981 aatcataaaa ttagctcata taaatatact aggatgtaaa atagactggt caaaatctgg
     8041 tcaaaaaaga ggactaatac aggtagtcct cttttttgtt attttaatat tctaaatcat
     8101 tttttattgg tttattccct tattcgatgt agactcaaat cactatatca ctagatatca
     8161 aacggcgtca tacggaatca aaaacatcaa atttgacctg ttaaatggtc attgaataac
     8221 attcaaaatc aataaaacgg agtcagacac ggagtcaaaa acgctgagag taaaaaatat
     8281 atacagcctt gtaggtaaag taccacaggg ctgtatattt tttatttcag attaccttaa
     8341 atgattccat ttacttttta tctcattttt tcctactagt tagtatcaaa tactctccta
     8401 ttttctcaat aaactatttt tggatacatg gtatctgaaa cagctaaaac cttgtcatta
     8461 cagcaaaaaa tcacgttat
//
LOCUS       DACCCS010000095         8290 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.100, whole genome shotgun sequence.
ACCESSION   DACCCS010000095 DACCCS010000000
VERSION     DACCCS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.100"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(45..1511)
                     /locus_tag="IVK82_002588"
     CDS             complement(45..1511)
                     /locus_tag="IVK82_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP6393601.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="IVK82_002589"
     CDS             1898..2494
                     /locus_tag="IVK82_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP6393602.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="IVK82_002590"
     CDS             complement(2552..3034)
                     /locus_tag="IVK82_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393603.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="IVK82_002591"
     CDS             complement(3140..3337)
                     /locus_tag="IVK82_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393604.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="IVK82_002592"
     CDS             3632..4390
                     /locus_tag="IVK82_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP6393605.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="IVK82_002593"
     CDS             4450..4878
                     /locus_tag="IVK82_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393606.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="IVK82_002594"
     tRNA            4924..4996
                     /locus_tag="IVK82_002594"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="IVK82_002595"
     CDS             complement(5146..5517)
                     /locus_tag="IVK82_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6393607.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="IVK82_002596"
     CDS             5732..5917
                     /locus_tag="IVK82_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393608.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="IVK82_002597"
     CDS             complement(6000..6188)
                     /locus_tag="IVK82_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393609.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="IVK82_002598"
     CDS             complement(6212..6475)
                     /locus_tag="IVK82_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393610.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="IVK82_002599"
     CDS             6633..6890
                     /locus_tag="IVK82_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP6393611.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="IVK82_002600"
     CDS             complement(6961..7152)
                     /locus_tag="IVK82_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393612.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="IVK82_002601"
     CDS             complement(7293..7613)
                     /locus_tag="IVK82_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393613.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="IVK82_002602"
     CDS             complement(7636..8070)
                     /locus_tag="IVK82_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6393614.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2628 a         1635 c         1314 g         2713 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg
//
LOCUS       DACCCS010000096         8035 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.101, whole genome shotgun sequence.
ACCESSION   DACCCS010000096 DACCCS010000000
VERSION     DACCCS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.101"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(53..787)
                     /locus_tag="IVK82_002603"
     CDS             complement(53..787)
                     /locus_tag="IVK82_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP6393615.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IVK82_002604"
     CDS             1114..1524
                     /locus_tag="IVK82_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP6393616.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IVK82_002605"
     CDS             1517..2218
                     /locus_tag="IVK82_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP6393617.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IVK82_002606"
     CDS             2218..3804
                     /locus_tag="IVK82_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6393618.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IVK82_002607"
     CDS             3823..4044
                     /locus_tag="IVK82_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP6393619.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IVK82_002608"
     CDS             4041..4802
                     /locus_tag="IVK82_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP6393620.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IVK82_002609"
     CDS             5182..5691
                     /locus_tag="IVK82_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP6393621.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IVK82_002610"
     CDS             complement(5766..6305)
                     /locus_tag="IVK82_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP6393622.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IVK82_002611"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IVK82_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP6393623.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..>8035
                     /locus_tag="IVK82_002612"
     CDS             7372..>8035
                     /locus_tag="IVK82_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAP6393624.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQ"
BASE COUNT         2463 a         1466 c         1641 g         2465 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acaga
//
LOCUS       DACCCS010000097         7760 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.103, whole genome shotgun sequence.
ACCESSION   DACCCS010000097 DACCCS010000000
VERSION     DACCCS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.103"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="IVK82_002613"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="IVK82_002613"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP6393625.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3273)
                     /locus_tag="IVK82_002614"
     CDS             complement(2032..3273)
                     /locus_tag="IVK82_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP6393626.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="IVK82_002615"
     CDS             complement(3291..4214)
                     /locus_tag="IVK82_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP6393627.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="IVK82_002616"
     CDS             complement(4435..6927)
                     /locus_tag="IVK82_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP6393628.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="IVK82_002617"
     CDS             complement(6934..7401)
                     /locus_tag="IVK82_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP6393629.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="IVK82_002618"
     tRNA            complement(7537..7610)
                     /locus_tag="IVK82_002618"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="IVK82_002619"
     tRNA            complement(7668..7741)
                     /locus_tag="IVK82_002619"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2161 a         1691 c         1247 g         2661 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DACCCS010000098         7397 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.106, whole genome shotgun sequence.
ACCESSION   DACCCS010000098 DACCCS010000000
VERSION     DACCCS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.106"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            68..151
                     /locus_tag="IVK82_002620"
     CDS             68..151
                     /locus_tag="IVK82_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP6393630.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            276..1013
                     /gene="erm(B)"
                     /locus_tag="IVK82_002621"
     CDS             276..1013
                     /gene="erm(B)"
                     /locus_tag="IVK82_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP6393631.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1183..1443
                     /locus_tag="IVK82_002622"
                     /pseudo
     CDS             1183..1443
                     /locus_tag="IVK82_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1606..2271
                     /locus_tag="IVK82_002623"
                     /pseudo
     CDS             1606..2271
                     /locus_tag="IVK82_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2307..2552
                     /locus_tag="IVK82_002624"
     CDS             2307..2552
                     /locus_tag="IVK82_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6393632.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2655..3524
                     /locus_tag="IVK82_002625"
     CDS             2655..3524
                     /locus_tag="IVK82_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP6393633.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3505..4239
                     /locus_tag="IVK82_002626"
     CDS             3505..4239
                     /locus_tag="IVK82_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6393634.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4272..5180
                     /locus_tag="IVK82_002627"
     CDS             4272..5180
                     /locus_tag="IVK82_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP6393635.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5177..5719
                     /gene="sat4"
                     /locus_tag="IVK82_002628"
     CDS             5177..5719
                     /gene="sat4"
                     /locus_tag="IVK82_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP6393636.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5812..6606
                     /locus_tag="IVK82_002629"
                     /note="aph(3')-IIIa"
     CDS             5812..6606
                     /locus_tag="IVK82_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP6393637.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6714..6902)
                     /locus_tag="IVK82_002630"
                     /pseudo
     CDS             complement(6714..6902)
                     /locus_tag="IVK82_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6882..>7397
                     /locus_tag="IVK82_002631"
     CDS             6882..>7397
                     /locus_tag="IVK82_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6393638.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIE"
BASE COUNT         2591 a         1118 c         1555 g         2133 t
ORIGIN      
        1 tgtgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg
       61 attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctactg ttttgaaaca
      121 gactaaaaac agtgattacg cagataaata aatacgttag attaattcct accagtgact
      181 aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat
      241 ttatttacag atgtaataac ttcaggagtg attacatgaa caaaaatata aaatattctc
      301 aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag
      361 aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta
      421 aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag
      481 aaaaattaaa actgaacatt cgtgtcactt taattcacca agatattcta cagtttcaat
      541 tccctaacaa acagaggtat aaaattgttg ggagtattcc ttaccattta agcacacaaa
      601 ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt gttgaagaag
      661 gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag
      721 tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa
      781 acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc
      841 tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa
      901 atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc gttacttatg
      961 agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg
     1021 agtcgctttt ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta
     1081 gatatactac tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg
     1141 tatcgataag gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct
     1201 aagtgtggca acaatattgt attaaaaaaa tcgttttatg gttgttcaaa ttatcctgaa
     1261 tgtaagttta ctttagctga acattttaga aagaaaaaac taaccaaaac gaatgtaaaa
     1321 gaattactgg agggaaaaga aaccctggta aaaggaatca aaaacaaaga gaaaaagccc
     1381 tacaatgccg ttgtaaaaat tggggaaaag ggatatattg attttatctc tttttcaaaa
     1441 taaacatggg gctgacccaa aagtagctcc tatatgaaaa atagaggtct ttccatttag
     1501 atagtagtgg aaagacctct atttatcttt tgaaagtgaa tacaaaaata aagcacactc
     1561 tgctataata taggtgatta atccatttga gagaggtgct ttatcatgta tcaaaattat
     1621 accataaacc aactttgttt gcctatagat cttgaaatta aattagaaga aaatgatttt
     1681 gctcatgcca ttgtgcagtt tgttgactct attcccgatg aagtctttct tccttattat
     1741 caatctatgg gtagacccca atatcatcct cgtatgctac tttccattat tctttgtgcc
     1801 tatattcaag gggtttattc gggccgccaa atacaaaata tgctgataga tagtattcgg
     1861 atgcgctact tatctcaaga gcaattccct aactttcgta ctattaatcg ttttcgcgtt
     1921 catccgatta tgaacgatat tctagatcat tcatttgttc aatttcgtga gctactggtt
     1981 caaagtggct taatcagtgg cagtgctcta tacattgatg gtacaaaaat tgaagcggat
     2041 gcgaataaat actctttcgt ttggaaaaaa agtattttaa agtataagga tagccttgat
     2101 caaaaagccc ttgaaaacta ccaacaaatg gtggaaacaa aaatcttacc tgagttaata
     2161 gatgatttaa aggatgaact ttctatagaa gagattgaaa ggattcgtca atctttggaa
     2221 gaaaaggaaa atcaattaat tgaagcgatt gaccaaaccg aaacagtaga agggggctga
     2281 cccattatta ataaggagtg gacaacatga ttgatgtaaa tgagctattg gaagaagcta
     2341 ttagagaaac tgaaaatcta aatgatggcg aagtttttct tgttaaagat ttattcaagg
     2401 gatatgtggg gaataggata cccagaaagg atcgacttct gcttggaacg ttatttttaa
     2461 accatgttag caaaatgaat ggtaatttaa aggctattga aaagacttca tctaatcagc
     2521 agaggtataa aaagacaatt gataagtttt aactatcttt tgtgaaatta aaaaaattat
     2581 aaatatgttg agtagaagca aaagatttgg cttggaattt ttacagcagt gaatcgataa
     2641 aaggagcatg cttgatggta gataatatta ttaaatcagt agcagagaaa ttatcctctc
     2701 tgtcttatat agaaggtatt gttttaggtg gttcacgtgc aaggggcacc catacagagg
     2761 attcggatat agatatcggc atctattaca attcagaatc atttgacata aatactatta
     2821 atcaattcgc tacaaagctg gatgatgagc atagaaataa ccttgttgta cctcccggag
     2881 catggggtga ttggattaat ggcggcggat ggttagtcat aaacgggtat catgtggatt
     2941 taattttacg tgatattaaa cgtgtggaac aaataatgaa agatacagag cacggaattg
     3001 ttactgccaa ttatcagact gggcatcccc atggttatat tagtgcaatg tatcgaggag
     3061 aattagcgat tagcaaaata ctatatgcta agaatgaaag cttatgcgaa ttaaaaaaac
     3121 aggcagaaac ttatcccaat gctttgcaga aaagtttagt taactttttt atgtttgaag
     3181 cagggttctc tttaatgttt gtaaaagcaa attcgggaac agacgataaa tattatattg
     3241 cgggtcatgt ttttcgtata gtttcatgtt taaatcaagt gttatttgca tgtaataatg
     3301 cttattgtat caacgaaaag aaagctataa aactgcttga aacttttgaa cataaacctg
     3361 aaaaatatac cgagaaggta aatcatattt ttgaagtact cggtatctca ctttttgaat
     3421 gctacgacat gaccgagaag ctttataatg aagtgaatga aattgtatcg gagataaata
     3481 actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa tatatttttt
     3541 caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg agaatgggag
     3601 actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt aggatgcggc
     3661 tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt aggtgttgat
     3721 atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca gattgaatat
     3781 gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt aatactaagt
     3841 tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat atataggatg
     3901 ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac tgctcatgga
     3961 acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt ggacaattat
     4021 tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa atatcataga
     4081 acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa tcagattgtg
     4141 gagccacagc cgccagagaa catgatggat attccgggga tggcggatga aatgcgacgc
     4201 ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa aaataaacga
     4261 ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct ttagcagaac
     4321 aggatgaacg tattcgaatt gtgacccttg aggggtcacg cgcaaatatt aatataccta
     4381 aagatgaatt tcaggattat gatattacat attttgtaag tgatatagaa ccgtttatat
     4441 ctaatgatga ctggcttaat caatttggga atataataat gatgcaaaag ccggaggata
     4501 tggaattatt cccacctgaa gaaaagggat tttcctatct tatgctattt gatgattaca
     4561 ataaaattga tcttacctta ttgcccttgg aagagttaga taattaccta aagggcgata
     4621 aattaataaa ggttctaatt gataaagatt gtagaattaa aagggacata gttccgactg
     4681 atatagatta tcatgtaaga aagccaagcg caagggagta tgatgattgc tgcaatgaat
     4741 tttggaatgt aacaccttat gttattaaag gattgtgccg taaggaaatt ttatttgcta
     4801 ttgatcattt taatcagatt gttcgccatg agctgctgag aatgatatca tggaaggtcg
     4861 gcatcgaaac aggctttaaa ttaagtgtag gcaagaacta taagtttatt gaaaggtata
     4921 tatccgagga tttgtgggag aaacttttgt ccacctaccg gatggattcc tatgaaaaca
     4981 tatgggaagc attatttcta tgccatcaat tgttcagggc ggtatccggt gaggtggcgg
     5041 aaaggcttca ttatgcctat ccggagtatg ataggaatat aacaaaatat accagggaca
     5101 tgtataaaaa atacactggt aaaaccggct gcctggatag cacatatgcc gctgatatag
     5161 aagagaggcg ggaacagtga ttacagaaat gaaagcaggg cacctgaaag atatcgataa
     5221 acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa acgagaattg
     5281 gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag acgaagagga
     5341 tgaagaggat gaggaggcag attgccttga atatattgac aatactgata agataatata
     5401 tctttactac caagacgata aatgcgtcgg aaaagttaaa ctgcgaaaaa attggaaccg
     5461 gtacgcttat atagaagata tcgccgtatg taaggatttc agggggcaag gcataggcag
     5521 cgcgcttatc aatatatcta tagaatgggc aaagcataaa aacttgcatg gactaatgct
     5581 tgaaacccag gacaataacc ttatagcttg taaattctat cataattgtg gtttcaaaat
     5641 cggctccgtc gatactatgt tatacgccaa ctttgaaaac aactttgaaa aagctgtttt
     5701 ctggtattta aggttttaga atgcaaggaa cagtgaattg gagttcgtct tgttataatt
     5761 agcttcttgg ggtatcttta aatactgtag aaaagaggaa ggaaataata aatggctaaa
     5821 atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg
     5881 gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa cctatattta
     5941 aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga aaaggacatg
     6001 atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga acggcatgat
     6061 ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa
     6121 gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag gctctttcac
     6181 tccatcgaca tatcggattg tccctatacg aatagcttag acagccgctt agccgaattg
     6241 gattacttac tgaataacga tctggccgat gtggattgcg aaaactggga agaagacact
     6301 ccatttaaag atccgcgcga gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa
     6361 cttgtctttt cccacggcga cctgggagac agcaacatct ttgtgaaaga tggcaaagta
     6421 agtggcttta ttgatcttgg gagaagcggc agggcggaca agtggtatga cattgccttc
     6481 tgcgtccggt cgatcaggga ggatatcggg gaagaacagt atgtcgagct attttttgac
     6541 ttactgggga tcaagcctga ttgggagaaa ataaaatatt atattttact ggatgaattg
     6601 ttttagtacc tagatttaga tgtctaaaaa gctttaacta caagcttttt agacatctaa
     6661 tcttttctga agtacatccg caactgtcca tactctgatg ttttatatct tttctaaaag
     6721 ttcgctagat agagttctat attagagatg taaagagttt tggtgaagag gtttgtgagt
     6781 atggctttta taaccagggt gcaatgagaa gcactaacag cagctagctt aagaacgcat
     6841 aggatatact ttttatggaa gtcacagagg aggaataaaa tttgaacagg attaacagag
     6901 taacctctat tcttattcag ctgcaatcaa aaaaaataat tcctgccaaa gaaattgcac
     6961 agcgatttaa tataagctta aggacagttt acagagatat ccggacactt gaagaagccg
     7021 gaataccaat tggatctgag gccggaaaag gatattttct tgtagagggc ttcctacttc
     7081 cgccggtaat gtttacagcg gcggaagtgg gtgcattgat tacagcaggg aaatttttaa
     7141 attgccatgg agatgaatca tttataaagg attttgattc agctatgtat aaaatcaaat
     7201 ctattttgaa gcatggtgaa aaaaactatg cacaggagtt ggagaacagt attaacgtgt
     7261 acagcacatc tggacagaaa aatacattgg cggataacgt tattgcagca attcagactg
     7321 caatctgcaa taaaagggta atatcaattc aatatcctgc atcaggaggg caagaaccgg
     7381 aaagcagaat gatagag
//
LOCUS       DACCCS010000099         7089 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.107, whole genome shotgun sequence.
ACCESSION   DACCCS010000099 DACCCS010000000
VERSION     DACCCS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.107"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(36..296)
                     /locus_tag="IVK82_002632"
     CDS             complement(36..296)
                     /locus_tag="IVK82_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393639.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(433..846)
                     /locus_tag="IVK82_002633"
     CDS             complement(433..846)
                     /locus_tag="IVK82_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP6393640.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1302..1787)
                     /locus_tag="IVK82_002634"
     CDS             complement(1302..1787)
                     /locus_tag="IVK82_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393641.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(1926..2171)
                     /locus_tag="IVK82_002635"
     CDS             complement(1926..2171)
                     /locus_tag="IVK82_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP6393642.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2364..3164
                     /locus_tag="IVK82_002636"
     CDS             2364..3164
                     /locus_tag="IVK82_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6393643.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3183..5051
                     /locus_tag="IVK82_002637"
     CDS             3183..5051
                     /locus_tag="IVK82_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6393644.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRSYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELCDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5044..5535
                     /locus_tag="IVK82_002638"
     CDS             5044..5535
                     /locus_tag="IVK82_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP6393645.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5523..6077
                     /gene="srlA"
                     /locus_tag="IVK82_002639"
     CDS             5523..6077
                     /gene="srlA"
                     /locus_tag="IVK82_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP6393646.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6095..>7089
                     /locus_tag="IVK82_002640"
     CDS             6095..>7089
                     /locus_tag="IVK82_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP6393647.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2492 a         1133 c         1370 g         2094 t
ORIGIN      
        1 aaataaaaaa cttccagcca aaactgactg gaagtttatt tttttctgaa atattctaac
       61 acaaatatga aaaagatggc cgaagctaat aagccgattt cgtagcaaaa caatgagata
      121 ccagagtcgt aataataaaa tcctctaatc ttatggataa aatagatcat cggacaagca
      181 gataagagta agtatttgtt aggccttttg aataagtaaa agaaagcact ggtcattatg
      241 ataaatggta tccaaaacaa aagtttactt aagacaatca aatgatcaac tcccatattt
      301 tttaccaaga gagatcataa catctttatc gtaaaaacaa aatatttttt tgctcatttt
      361 gaaaatttaa acatactcgt tcttatccaa atatataaaa atcgcccctc attcatcgag
      421 aggcgataag ttctaattta actcaatttt ttttgtttct tttggctgat aagctgtttt
      481 aggtaatacc aatttcagca caccgttttt aaaggttcca gtgatttttt cttcatcaac
      541 attaggaaga tagaatgaac gtttgtaaga gaccgaatgt ctttctttcc gtacatagtt
      601 gccatcttct ttatcttctg tatgggactc atgattggcg gagatcgtca aagtattatc
      661 tgaatattca actgtcgtgt cttctttgtc acagccaggc atatcagcag ttagtttgta
      721 ttctttttca ttttcgacta aatctacttt tggataactt gaaacaccta aaaaatcatt
      781 gaatgctgga ctaaacacgt cattgaaatc agggaacatg tcgcgaatgc taggtacatt
      841 tgccattttt gatcatcctt tcaataagta ttttttattt atccctattt acgtaataac
      901 tctattttga aaaaaatgca agtataatgg ttagctttgt gaatatttca tctaatacga
      961 tacaaaggct atctttctaa tttgaaaaag ggatcattaa aataataaga agaaaatatg
     1021 tcaaaaggta aaaaagtaaa ttcttctatc caaatataga aaaattgaat ctagaaaacc
     1081 gcccgacaat aaatttcatt aactcagttg accgaaaatg aagtagatac caaaaattta
     1141 tcaagagagc aagtagagaa gtgagtatat tcataagaga aatagctgta acgaattatt
     1201 aggttaatgc aaatgcttat ttatactccg tataactttc tcagaaaaaa tgataccaat
     1261 aatgacttgc tagtactgta aatccgtata atatcatcta atcaaaatgg tgttccattt
     1321 gcatctgtca tcgtaatcga tccttcgggt gacactcgat aaaaaccaac gcttccagat
     1381 cctccttggg aacggatttg tttgctggaa gctgtgaaca aataatctcg atcaattttt
     1441 tgataaaagc taataacaat atcgtcactt tgtaattcgg aacggtgggc aataagaaat
     1501 agaagtgctt tgtcagagtt ttctaatgga agagaaggta cagtgctttc ttgcatttca
     1561 gatgttgaag aaatgctaaa cgactgaata cttgattggc tatgttgcga atctacatta
     1621 ccagtagaag tattagtaat ggaagtagta gaagtacttt tagttgaacc aacctcagtt
     1681 gttgaaatgg ctgctgaaga ttctttaaca gaattctttg ttttatatcc acaggcagaa
     1741 aaaagtatta cagttcccaa caagcctgcg taaatccctt tatgcatgat agctcctcct
     1801 aaaatagatt atctttattc agtatatcac ttaaacaaag aaaagcagat ggtttaaaat
     1861 gaaaaaacct tcccaataaa gggaaggttc atgaagataa taactttttt aataattata
     1921 gctttctaat atctttcatc aacttttcta gtaatcattg ggaaaaattt caagcagtag
     1981 atcgcacaaa tacctacgac gatataaatg atcttcgcaa aaatagttgt tgaaccacca
     2041 gcaatcatag ctactaaatc aagttcgaac aagcctacca acagccagtt caaccctccc
     2101 acaatcaata aagttaatgc aatagcatct aagttttcat tagaattctc ctttcttaaa
     2161 tgataaaaca attatatatc aaaaagagaa acattgctta tataacgctc aggaatttta
     2221 gcaatctatg caaaaagatg aaaatttttt ttgctcttcc attagtacac attttttagc
     2281 tgaagctgta aatttcatgt cttaattttt tttagtgagc ttttatactg gatttgtaag
     2341 aaaaaagaag gagaggatca catatgtcgg attggttgga tattgcagga aaagtagtaa
     2401 ttgtaactgg aggttcctca ggaattggaa gaagtatcgt cgaaaatttg ttgaagcaga
     2461 atgcacaagt agcgaatttt gatgtaactg aatgccgtat acagcatgaa aacctactat
     2521 ctttaaaagt cgatgtgtct tctaaaacgg acatagaaga agggatatat aaagtaatga
     2581 aacatttcaa gacgatcgat ggtctagtca ataatgcagg aataaatatt ccttctcttc
     2641 taatagatag aaatcatcca aaaagcaagt atgaattatc agaacaagtt tttgataaga
     2701 tgatcgctgt caatcaaaaa agtgtatatt tgatgagcca agcagttgga agaatcttgg
     2761 tgcaaaaggg atcaggagtg atcgtgaatc tttcttctga atcaggattg gaaggatcag
     2821 aaggacagag ctgttatgct gctacaaaag cagcgatgaa cagttttact cgttcttggg
     2881 caaaagagtt aggaaagagg aatgttcgtg tggtaggtgt tgcaccaggg atactggaag
     2941 aaaccggttt gagaacacaa gaatatgaag aagctctttc ttatacgaga ggtataagcg
     3001 tcgaacagct gagaaacggt tacagtcgaa caagtacgat ccctctagga aggagtggta
     3061 agctgcaaga agtggcggat ctagtctgct attatctttc agaaagatca agttatatca
     3121 ctggagtcac gactaatatt tcaggaggta aaacaagagg ataaaggata ggggggacgc
     3181 acgtgacgct agtaaacaga tggtaccaat tattgaaatt actagtcact cacaagaaag
     3241 tttcaatcca ggaagtgcaa aaagaaatca aaaccagtaa tcaaaccata aaaaaagata
     3301 ttggggagtt gaatgaagaa attgccggta ttgcaaaaat catccaaaaa aacaaacatt
     3361 ttgaacttga aatttatgat tttgattttt ttgatgagat catgcaagga aaattgaaaa
     3421 cagccagtga tttcaattcg gcatccaaaa gaatcgctta tttactaaat aggctgatcg
     3481 aaacagagga tttcttacgt atggatgatt tgtctgaaga agtcggtgtc agcagaggaa
     3541 cagtggtcaa ggatctaaag acattaaaag agatgatcaa tgattttgat gtaaagatta
     3601 caggcacacc aaataaagga ctaagaataa taggagatga gctagaatta cgactacttt
     3661 tattttattt tgtttctccc tacttttcag agacgaaaac agaacagttt ctagaagaaa
     3721 aacaagcaat caaacatttt cagaagaaga cgaatgctag taaacaagaa atcacccttt
     3781 taacgaaagt tatcggtatt tctttgaata gaatctctag taactatctg ttgaagaagc
     3841 ccatcaatcg ttacagtggg tgttttgaat atatccaaga attcaatgaa ctgatcaatc
     3901 agttggaaga aatctatgaa ctaactttga gccaatatga aaaagatttt atcagttttc
     3961 ctttgaacat catcaacaca agtataacga tgataaggaa agctaactct gatttgcgct
     4021 tatatttcga ccccatgatg atgcgtattc gacgattaga tctcattgat ctggatgagt
     4081 ccttcttata tcaagagatg aaacagcacc tctctttatt gataaatcgg gtaatttttc
     4141 gttatcgatt gactgattta ttttatggtg agatagaaaa aaagtatcct ttttcttacc
     4201 aaatcgctag cgaatgcata caagcattag aagctatctt gcaacataag atctctaaag
     4261 tagagatcag ctacctaacg ttgtatttgg agttagcatt tcgatatcaa aaagagcatt
     4321 tttcctctaa aaaagtagcc attgttcata cgacaggaaa aggaacagca ttaatgatcc
     4381 aaagacaaat caaaaggata ttggggaggg atatacaaat aacacatgtt tcagaagaac
     4441 aatatgaaaa agtagatcta aacaaatatt tcgctgtttt tacaacgatt ccgttaaaaa
     4501 atgtaaatga agagacaccg gtgatccatt tgactaatct ttttgatgac tcttggttaa
     4561 gggcacaatg gcaaaaagca aaaaaaaagc gattagccgg gtttcattct actgtttttt
     4621 tctatcaaat atggaaagaa aaaaagacgt atcaagaata tttaaacaaa ttagtcgctc
     4681 ctttgatcag aagctattat gtggatgaag aatttgaaaa aaggatcttg aaaagagaat
     4741 atcagcaaag cacatttgtt aacaaagaat ttgcttttcc tcacacaacc aaccagctat
     4801 ctcaacacat tgttttatca gtagggctaa taactgatca aggtttagca gaaacaggag
     4861 gaaatggagt tattttttta tttgccatac ctgaagaaat gacggctgaa gttgagaatg
     4921 aattatttga gctatgcgat atcctatttg aactcataaa aatgggaata gatgagttta
     4981 ttgaattagc accgacacaa gaatcatttt taaaactaat caaaagtaaa ggagagagac
     5041 ttcatgaatg atatgtatct attcatagtt ttcgcagctt cagcttatgt gctgcaagca
     5101 gttatcagtt ggaagcaact aaagcatttc aatcaaacat atagcgaact gcgaagaaaa
     5161 ggaaaagtag cgattggtaa acgacctggg aaaatccgtt caggcacgat tgttatgcta
     5221 gctatagacg atcatgatca agttttagaa gtcagaaaaa tgcaaggggt tacatctttg
     5281 gcaaaatttc attcattacc tgtctatgcg ggtgaagata tccattacat cgatcgttac
     5341 catccctcag ttcgagcgga agatcgtttg acacaagcag ctttagaaaa tgccagagaa
     5401 gtatacttac gaatagaggc aggaaatgaa aaaagtgaac aaccttcatt gccttttatt
     5461 gctatgctca ataattttcg gatatggaaa agtacgatca aagaaaaaag gagtgacaaa
     5521 cgatggatat cataacaaaa tttgctgagg gatttatgag gctgttccaa acaggtgcag
     5581 aaacattcat aagctggatg acaggaatcg taccagttgt cttgatgctg atggttgcaa
     5641 tgaatacgct gattgctttt ttaggtgaag accgagtaac gaaagtagcc aaattatcag
     5701 cgaaaaatcc gcttactcgt taccttgtgt taccgttctt atctgctttt atgttgggaa
     5761 atcctatgtc atttacaatg gctcgtttcc ttccagaata ctacaaacca agctattatg
     5821 ctgctcaagc tcaattttgt catacgagca atggtgtatt cccccatatt aatcctggag
     5881 aattgtttgt atggatgggg attgctcaag ggatcgaaac cttagggcta aattcgatgg
     5941 agctggctat ccgatacttg cttgtgggac tgctgatgaa ctttattggt ggttggataa
     6001 ctgattttac tactggattt gtatgcagac agcaaggaat cgttcttagc aaaaaagtag
     6061 agctttcagt tgattagaga aaggagagtt ttaaatgtat ggtgatatta agatcgtaag
     6121 aggaaacggc gggtatggag gtcctttgat tattaggccg acaaaagata aacacaagtt
     6181 catctatgtc acaggtggag gtgagaggcc aatgatcgta gataaaatag aagaactgac
     6241 aggtatggag tctatcaatg ggttcaaaac atcaatacct gatagtgaaa tcgcgcttgc
     6301 aatcgttgac tgtggaggga ctcttagatg tggaatttat cctaaaaaag ggataccaac
     6361 catcaatatc gttcccacag gaaaaagtgg cccattagca caatatatca ctgaagatat
     6421 ttatgtttca gcagtagggt tagaacaaat acagccacta atggaaactg atgataacaa
     6481 agaaacagta aatactgcat ctccagataa aaagtacaaa tatgatactt ctaaaaagat
     6541 aaccgagcaa aaagcagagc agcaaaactg gatagcaaaa attggaatgg gcgcgggaaa
     6601 agtagttgct acattcaatc aatcagcaag agaagcagta gatacgatga tccggacagt
     6661 gatccctttt atggctttcg tttccctttt gattggtgtg atacaaggtt ctgggatcgg
     6721 taatgtattt gctaacatta tggcaccttt agcaggaaat gcagttggat taacaatcat
     6781 tggctttatt tgttcactgc catttttatc tcctttgctt ggtccagggg cagttattag
     6841 ccaagtgatt ggaacattga tcggtgtaga gattgggaaa ggaaatattc ctccccaatt
     6901 agcattaccg gcactatttg ctatcaatac tcaaaatgca tgtgatttta ttccagtcgg
     6961 tctaggtttg gaagaagcaa aagctgagac agtagaagta ggtgtaccat cagttcttta
     7021 ttcaagattt cttaacggca tcccacgagt tatcattgct tgggtagcaa gttttggatt
     7081 atatagctg
//
LOCUS       DACCCS010000100         6940 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.108, whole genome shotgun sequence.
ACCESSION   DACCCS010000100 DACCCS010000000
VERSION     DACCCS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.108"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            127..579
                     /locus_tag="IVK82_002641"
     CDS             127..579
                     /locus_tag="IVK82_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393648.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IVK82_002642"
     CDS             593..1282
                     /locus_tag="IVK82_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393649.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="IVK82_002643"
     CDS             1407..3758
                     /locus_tag="IVK82_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP6393650.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="IVK82_002644"
     CDS             3838..4233
                     /locus_tag="IVK82_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393651.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="IVK82_002645"
     CDS             4558..5175
                     /locus_tag="IVK82_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6393652.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="IVK82_002646"
     CDS             5216..5515
                     /locus_tag="IVK82_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393653.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="IVK82_002647"
     CDS             5549..5716
                     /locus_tag="IVK82_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6393654.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6324)
                     /locus_tag="IVK82_002648"
     CDS             complement(5761..6324)
                     /locus_tag="IVK82_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6393655.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(6352..>6940)
                     /locus_tag="IVK82_002649"
     CDS             complement(6352..>6940)
                     /locus_tag="IVK82_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP6393656.1"
                     /translation="QELLYDRGINVCHTTIYRWVQEYSKVLYYLWKKKNRQSFYSWKM
                     DETYIKIKGRWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTD
                     KAPSLGSAFRKLQSVGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIK
                     GMETIRGIYKKNRRNGTLFGFSVSTEIKVLMGITA"
BASE COUNT         2337 a         1007 c         1368 g         2228 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttcggt
     6301 tctgttgcga agtttgaaaa tcaaacgcgc cctctctgaa ctccaaatat cttaggctgt
     6361 tattcccatt aataccttga tttcagtaga caccgaaaag ccgaagagcg ttccatttct
     6421 tcggttcttt ttatatattc ctcgaatggt ctccatgccc ttaatcgtgg aagaggctgt
     6481 acggagactt tgataaaatt tatttcgtcg tttaataggt cgatggtctt gttctattaa
     6541 attgttaaga tacttcacag ttcggtgctc tgtcttagta tataaaccca cactctgtaa
     6601 ctttctaaag gcggagccaa gagaaggtgc tttatcggtc acaattgctt tcggctcacc
     6661 aaactgttta tggagtcgtt ttaagaaagc ataggctgct tgcgtatccc gtttctttcg
     6721 taaccagata tctaaggtta agccgtccgc atcaattgca cgataaagat aatgccaacg
     6781 tcccttaatt ttgatatagg tttcgtccat tttccatgaa tagaaggatt gtctattttt
     6841 cttcttccaa agataataga ggactttgct gtactcttgt acccaacgat aaatcgtagt
     6901 atgacaaaca tttattccac gatcatataa caattcctga
//
LOCUS       DACCCS010000101         6467 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.109, whole genome shotgun sequence.
ACCESSION   DACCCS010000101 DACCCS010000000
VERSION     DACCCS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.109"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(109..564)
                     /locus_tag="IVK82_002650"
     CDS             complement(109..564)
                     /locus_tag="IVK82_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393657.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IVK82_002651"
     CDS             complement(718..1926)
                     /locus_tag="IVK82_002651"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP6393658.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IVK82_002652"
     CDS             complement(2038..2370)
                     /locus_tag="IVK82_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP6393659.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IVK82_002653"
     CDS             2518..3393
                     /locus_tag="IVK82_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6393660.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IVK82_002654"
     CDS             complement(3475..4056)
                     /locus_tag="IVK82_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP6393661.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IVK82_002655"
     CDS             complement(4062..5321)
                     /locus_tag="IVK82_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP6393662.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IVK82_002656"
     CDS             complement(5323..6369)
                     /locus_tag="IVK82_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393663.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1804 a         1337 c         1102 g         2224 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaa
//
LOCUS       DACCCS010000102         6334 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.110, whole genome shotgun sequence.
ACCESSION   DACCCS010000102 DACCCS010000000
VERSION     DACCCS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.110"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1002
                     /locus_tag="IVK82_002657"
     CDS             <1..1002
                     /locus_tag="IVK82_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP6393664.1"
                     /translation="RQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGK
                     RGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGL
                     KHHVISHYVEESISELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIR
                     CERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQST
                     FGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILL
                     SYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            1013..3022
                     /gene="topB"
                     /locus_tag="IVK82_002658"
     CDS             1013..3022
                     /gene="topB"
                     /locus_tag="IVK82_002658"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6393665.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            3368..3964
                     /locus_tag="IVK82_002659"
     CDS             3368..3964
                     /locus_tag="IVK82_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6393666.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            3977..4876
                     /locus_tag="IVK82_002660"
     CDS             3977..4876
                     /locus_tag="IVK82_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP6393667.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            4879..5010
                     /gene="ssb"
                     /locus_tag="IVK82_002661"
     CDS             4879..5010
                     /gene="ssb"
                     /locus_tag="IVK82_002661"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6393668.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            5032..5364
                     /gene="ssb"
                     /locus_tag="IVK82_002662"
     CDS             5032..5364
                     /gene="ssb"
                     /locus_tag="IVK82_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6393669.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            5409..5642
                     /locus_tag="IVK82_002663"
     CDS             5409..5642
                     /locus_tag="IVK82_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393670.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(5801..5923)
                     /locus_tag="IVK82_002664"
     CDS             complement(5801..5923)
                     /locus_tag="IVK82_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP6393671.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
BASE COUNT         2272 a         1105 c         1342 g         1615 t
ORIGIN      
        1 cggcaaaatt ttttagttgg agacgaccgt ttagatcatg cggttgcttt tttatggaaa
       61 gatccacaga caggagagac agtaggggca agttatcaag gaacgatcgt cgattttaat
      121 cgtttcggta aacggggaac gtataaacac attgacaaga atcctacgcc aaatcacgga
      181 tttaatttaa aaataggcga tccgaagcat ttaaaattct ttgaaagtag tattgatctg
      241 ttaagttacg ctgcattgaa tcgggagaaa ctacaagatg cctggttagt ttcgatggac
      301 gggttaaaac atcatgtaat cagtcattac gttgaagagt cgatttctga attaagacga
      361 aaacaaacat ttcctcaatc gattgaaata tgtgtggaca atgatcgtgc aggacatatt
      421 ttttatgaaa aagaacagat gaagggaatc gttgatcctt ttaccaataa gaaaatccgt
      481 tgtgagcgag gaattccaaa tgattggcaa gtacctaaag aatacaaagc tacttatgaa
      541 gcagtcgcaa aagaaatgag tgttgaacct gaagcaatta tggccataca caaaactgaa
      601 actaatttac agctgacaaa tcaattggtg tctgctcatg atgttcaatc tacgtttgga
      661 aagatgttgg cgaagggcga accagtagaa acaattgatt tgaaagaagc atgtaccacg
      721 gtcgcgaagg aactgaaagt atgtgaacgc gcagatggaa catacaactt tgatcgtttt
      781 tatagtagaa aagccaacat aaaagacgtt aatgcaggaa tccttctttc ctataaagca
      841 gaacaatact acaaaggcta taaaaagcat gaacatgaat ttgtacctga agtgaaaaag
      901 gattggaatg atcaattgaa gcatgagatc cagcaacaag aaatcagaaa gcaaaaacgt
      961 gcgatgttgt ttcaacaagg cagacaacaa gaaagggagt gaagtcgtca ctatgaaaac
     1021 cgtcatttta gcagaaaaac cgtcacaagc gaaggcttat gccgatagct tttctaaagc
     1081 tacccgaaaa gatgggtatt ttgagatcca agatcgattg tttacaggag aaactgtgat
     1141 cacgtatggt tttggtcatt tagtcgagtt agattcgccc gatatgtacg atgaaaaatg
     1201 gaaacaatgg gcattagaac atttgcctat tttccccaat caataccact atcatgttcc
     1261 taaagataag aaaaagcagt ttagtgtcgt caaacgacag ctgcagtcag ctgatacgat
     1321 tgtgatcgcg actgatagcg atcgtgaagg ggagctgatt gcctggtcaa tcattcaaca
     1381 agcaggcgcc aatcaaggaa aaacctttaa aagactttgg atcaactcct tagaaaaaga
     1441 agccatttat cagggattcc aacagttacg agacgcagaa gagacctatc caaaatttga
     1501 agaagtacaa gcacgtcaga tcgccgattg gttaattggg atgaacggta gcccactgta
     1561 tagtttactc ttgcagcaaa aaggcatacc aggaagtttt tctttaggta gagtccaaac
     1621 cccaacactc tatatgattt atcaattgca ggaaaagatt agaaacttcc aaaaagaacc
     1681 atattttgaa ggaaaagccc aagtaatcgc tcaaaatggc gcatttgatg cgaagcttga
     1741 tccaaatgaa acccaagcga cacaagaagc atttgaagcc tatttaaaag aaaaaggtgt
     1801 tcaattagga aaacagccag gtacgatcct tcaagtcgaa acagagaaaa aaagtgcggc
     1861 tagcccacgt cttttttctt tatcgagcct tcagtctaaa atgaatcaac tcatgaaagc
     1921 tagtgccaaa gatactttgg aagccatgca agggttgtat gaaggaaaat atctaagcta
     1981 tccgcgaacg gatacccctt atattacgga aggggaatat gcgtatctgt tggatcactt
     2041 agacgaatac aagcattttt taaaagccga agcgattccg actcctattc atacaccgaa
     2101 tagtcggtat gtgaataata agaaagtcca ggaacattac gcgatcattc ctacaaaaac
     2161 ggttatgaca gcagcggctt ttgaacaact aagtccactg cagcaggcta tttatgaaca
     2221 agtgttaaaa acaacggtgg ccatgtttgc ggaaaagtat acctatgaag aaacaacgat
     2281 cttgacgcaa gttcagcagc ttcagttaaa ggcaatcgaa aaggtgccgc tagatctagg
     2341 ttggaagaag ttatttggaa aagagagcga cggaaaagag aaggaagaag agccgctact
     2401 tcctaaagtg acaaaaggag aaatggtgac ggtggatcta caagttcttg aaaaagaaac
     2461 caagccacca caaccttata ccgaaggtac gttgatcact gcaatgaaaa cagctgggaa
     2521 aaccgtggat agtgaagaag cgcaatccat attgaaagaa gtggaaggaa tcggtacaga
     2581 agcgacacgg gcgaatatta ttgaaacctt gaaacaaaag gaatatatca aggtagagaa
     2641 aaataagctc gtggtgacga ataaagggat tttgctttgc caagccgtgg aaaaagagcc
     2701 gctgcttacg agcgccgaaa tgaccgccaa atgggaaagc tacttgttaa agatcggtga
     2761 acgaaaagga acacaaacca cttttctagc caatatccaa aaatttgtct ctcacttatt
     2821 ggaagtcgta ccagggcaaa tccaatccac tgattttgga tcgacgttac aagaagtgaa
     2881 agcagccagt gaaaagcaag aagccgcgcg tcatttagga atttgtccaa aatgccagga
     2941 acaagaagtc ttactctatc aaaaggtggc agcttgtacg tctgaagcct gtgattttaa
     3001 aaggtcagaa gggttcattt gaagcaacga ttgttttaaa agaagacttc actacaggct
     3061 ttgaattttc tgaaaagaaa aaaacgaact tcaagaaaag aacacgccgc actgcaaaat
     3121 aatggagggt taatcttatg cgtaatacca cacacaaaca acaagaaatc gaaaatcaaa
     3181 aagccaaaat tcgttcagaa caggaaaaaa tgcgtgccag caaacgaaga ctgcagcaat
     3241 tgaagaacca gcagaggatt caaaaagaaa tcgaattaga tcgataaaat cttcctaact
     3301 attgtatacg tataaactta tgttatactg tttttataag tttatacgta tacaaaggag
     3361 ggagaaaatg acaaaagcaa tggtatacaa gctgctagaa gaatttaatg gaactttggc
     3421 tttgaaggat gccaaaaaag cgggcatttc tcctgtaacg atcaaacgta tggtagatcg
     3481 aggggagctg gatcgagagt atccaggttt tttcacctta cctggtcaat ttcctgatga
     3541 attgtttatg gcgcaaaaaa aatatgaacg cggcattatt tcacatatta ccgcgttaga
     3601 tttgtatgat ttaacagata tgatccctag acagatcgat ctgactgtac cttacggtta
     3661 tcacgtttct gaaaaaggat tgaaggaatt cgcagttgag ctacactata caaaagcgga
     3721 atggtatgaa ttaggtaaga ttgaaataaa aagtcgctat gggaatccta ttatagctta
     3781 tgatccagaa aaaaccttgt gtgacatttg gaatccctgg tataacgttg aggatgaaat
     3841 caaagtaaaa gcaatcaaaa actacatgga gtcggacaga aaaaatctta gaaaattgaa
     3901 tgagtatcga cgaattttgc ctacagataa aacgatgcgc tcttatatta tggctttaaa
     3961 ttaaaggaga atacacatga cacctacgca attaaaagca aaagtgaaga atatttctag
     4021 agaaaaagaa gttgatccgc agcttgtttt aagacatttt atgatggaaa aattcttgga
     4081 aaaaatctca gagtctcctt atcgtgaaaa ttttgtgtta aaaggcggct tcttaattgg
     4141 ctcaaagtat ggcattgaaa atcgaacaac taaagacatt gatacgactt tacgggaaat
     4201 gaaagtaacc aaagaaacat tgacaacggt tctaaacgat attttttcaa cgccgacgaa
     4261 agaaggcatt caatttgaga ttcaaggaat gaaagaaacg agggaagctg attattatcc
     4321 agggtttagc ttacgtgtac tggcacattt ggaaaacatg cggccagatt ttaaggtaga
     4381 tgttactaca ggtgactcga tctatcctgc gacgatcact cacagtcata aattgatgtt
     4441 tgaggatcga acgattcctt tggaatccta tcctacagaa caaattattg ctgaaaaatt
     4501 aagtgcaacc tttgattttt taacagataa ttcgagagga aaagattttt atgatctcta
     4561 tactattcct aaaatggaga agatcgatga gaagaccttg aaagatagtg tgagaaatac
     4621 atttacacgt cgaggaaaaa ctgatccgtt aaagatatac tatgaaaagg atatggatgt
     4681 actgaaaaat agtcacgagc taaaagatac ctggaagaaa tatcaaaaaa gcaatcctta
     4741 tgctgcgtcg atcacgtacg atgagacaat ggattcgatt agtaatttga tggaaaaagt
     4801 gattcacgtc gaagaaaaag agcgaaaact agcttatcaa agacaccaac agatgaatag
     4861 agaaatggag agataaagat gattaataat gtgacattag taggacgctt aaccaaagat
     4921 cctgatttac gttataccgc aagtggtact gcagtagcga cttttaccct agccgtcaac
     4981 cgaaatttca cgaatcaaaa cggcaattga gaagccgatt ttattaactg cgtgatttgg
     5041 cgcaaaccag cagaaacaat ggcgacatta gctaaaaaag gtagcttgat tggtgtagtt
     5101 gggcggattc aaacgcggac ttacgacaac cagcaaggac aacgtgtata tgtgactgaa
     5161 gttgtagcag ataactttca actattagaa agtaaagcag cgacagagag ccgtgcgcac
     5221 gctgatcaat cgtccaccag tccaagcacc gccacctttg aacaacggaa tacagcgacg
     5281 cctaacaaca atggattgaa cgcttcacag aatccatttg gtggacaatc aatcgatatt
     5341 agcgatgatg atctaccgtt ttagtttgag aaagaaggag taagcatggc aaaaaaatta
     5401 gtctgacgat ggatctattg atacccgtaa ttgcatttat agacgggatt tctttgggcg
     5461 tcactgaaaa tcctttggta aatagaattg cgaaaggatc actatttctt gtattcgtgt
     5521 atcttgtctg ttcttatctt ttaaaaagag caggaaagga taattccttt tggcgattga
     5581 ctaaagtcac agtagcagcg acagctgtta tacttacggc tattctaccg ctttatatgt
     5641 agagttaacg ggaaatcgtt gaacttcttg cgaaatattg ctataatgaa catgaaaaag
     5701 aggagttgcg ctaacaaccc ctcattaaca gcaccgatta aagaacggtg gcagaatatt
     5761 agttatttgg aaataaccgc tgacctgcta aagtagacgg ttatttcttt ttgtcatttt
     5821 taagcatatt tacaatcaaa ctaatcaaag caatggtaaa cataccaaaa ccaagaatgg
     5881 tttgaattgt ctcatatgcg gacaaaaggc ttactccttt catagatttt agcacttaca
     5941 ttcataagca ccaccacctt tccagaagtc gccaccgtct tcaaacttta ctgtcttgac
     6001 tattatagca tatctaaaat cgaataaaaa ttctcaattt tagctgtttt tgctataatt
     6061 ttagcgaggt gatcgtgatg gaacaatata ttggtttact cgataaatta gggtgatcaa
     6121 gacacagcca ttgcttgtgc gatttacatt acgcacgagt catgaaccaa tcaattgtgt
     6181 agtggccaat atagaaatag cggataaatt gctgattttg gaagaaggca agtatactat
     6241 ttcagtgaca ggtcacttca ataaacgaaa gcaactagtc attgaatcaa tgcaaattag
     6301 aaaccctgat cattttacgc gatcgatgag cata
//
LOCUS       DACCCS010000103         6261 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.111, whole genome shotgun sequence.
ACCESSION   DACCCS010000103 DACCCS010000000
VERSION     DACCCS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.111"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..404
                     /locus_tag="IVK82_002665"
     CDS             <1..404
                     /locus_tag="IVK82_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP6393672.1"
                     /translation="ESLSTKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSDY
                     RQFDNWYKKFNYAIKSAEKEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAKT
                     DHRIKQKNNYIFTSLKNKALELIKLQKKEGI"
     gene            406..624
                     /locus_tag="IVK82_002666"
     CDS             406..624
                     /locus_tag="IVK82_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393673.1"
                     /translation="MIENHLVDKIHCLDQNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            611..3148
                     /locus_tag="IVK82_002667"
     CDS             611..3148
                     /locus_tag="IVK82_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393674.1"
                     /translation="MKIIKKKIANRKTNWLPVIFLVLIAVILFTSNIAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQAVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVEGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVA
                     YVCAVIVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVYAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEVLINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENLQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVYVRENVQSSGTSQPRTVDV
                     RENLQNTTSSQNGNRTVDVRENVQSSASNQPRTVEVRETRAEASQGDKTQKINIIEER
                     KERPNEKVIVEQEVKEKKRPRFTYENNPLFQDISDKPNPLFDSLFKK"
     gene            3162..3350
                     /locus_tag="IVK82_002668"
     CDS             3162..3350
                     /locus_tag="IVK82_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393675.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            3362..3973
                     /locus_tag="IVK82_002669"
     CDS             3362..3973
                     /locus_tag="IVK82_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP6393676.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            3958..5349
                     /locus_tag="IVK82_002670"
     CDS             3958..5349
                     /locus_tag="IVK82_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP6393677.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDNSEE
                     ALQEMNLVRTRMNKSEDIAQAFHVIHSFDKNISKELSIEKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNIDMETCKRLRIDPKDLERMRKINDEVLVENGLEPVDNQY
                     YENLEKRTNLYSNDPSKLKNGKINYQEFVQNAIEATLENCEVNSFVRFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRDELITLGREKQSRKEKSQYVERTFSARKLGK
                     KYSFESVFERLEKNLEETHMNNFVDLDTEQIIALASGVDYKTGELNVPFVELDSIAQK
                     QFSDHEGAKKIIKYDDFLREVDRHYANTISLEDDQEYTIKLHTDVNPRTLRSTKSKTI
                     LVKKTDQKELFLPAEGFKNCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQRRQRDFEMDF"
     gene            5565..5867
                     /locus_tag="IVK82_002671"
     CDS             5565..5867
                     /locus_tag="IVK82_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393678.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            5860..6207
                     /locus_tag="IVK82_002672"
     CDS             5860..6207
                     /locus_tag="IVK82_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393679.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKRLFVKSCW"
BASE COUNT         2372 a          889 c         1258 g         1742 t
ORIGIN      
        1 ttgaatcact ttcaactaaa aataataaat tagatgaaga attgatcgaa acatttattg
       61 aagaaaatct tcttaaacaa atgtggggag aaagtattgt gaattgctta aaactggcta
      121 gcaatagtga ttatcgtcaa ttcgacaact ggtacaaaaa attcaattat gcaattaaat
      181 ctgctgaaaa agaacaaaaa gttcaactga aaattatcta tgaaatttgt aataatagtt
      241 attttgtaga tcatgttaga gaacagctag caatgacttt acgtgacttg atacgaagag
      301 caaaaacaga tcatcgtatc aaacaaaaaa ataattatat ctttacttct ctaaaaaaca
      361 aagcgttaga actaatcaag ctacaaaaaa aagagggaat ttaaaatgat tgaaaatcat
      421 ctagtagata agattcactg tcttgatcaa aacgcagata ctcttgagtt aaccgagagt
      481 atctgctcaa atactattgt tctcgattta aaagctgtta atgatgatta cgtaactcat
      541 atcataattg acagaaaaat agctcagaaa ctctctgaga gcctaagaaa atgggctgag
      601 ggaggtaaca atgaaaataa ttaaaaaaaa gattgctaat agaaaaacaa attggttacc
      661 ggttattttt cttgttctaa tagctgtgat tctttttacg agtaacattg cagaaggcag
      721 tattttcaat tggtttgatg atactgagag tcaaactgag tttttaacta atccgcttta
      781 ccagaattat ttgaaaaaat acaacggaaa tttttacagc cagttcggta tgtggatagg
      841 ctggtctgtt ataaaatcaa tgttcgctgt tgtcgatggc attcaaaata tgattcccga
      901 agttttagat ttgtttagct ttgtagaaag taccggattg aacaaagtct atcaagctgt
      961 tttaagtacc attgttgttg gcttaatgat actttcacta gtgattattg gttataaaat
     1021 gattacaggt aagggaacgg ttgatctaaa atctgttgga atgaatgttg taatgtcaat
     1081 ttcattgatt cttttaatgc caacgttgat tcacagcgga atagagtttt ctaaaacatt
     1141 ttataatgat acgactacga ttacaaattc ggacgaaggc gtggcgtggt cgctgattga
     1201 acaaggggtt actgatttag cttatatcaa taaaacagat caatattcca tgatcgataa
     1261 aacgaatgac cggaacaatc taacaaaaga aaatttttgg aaaacggatc ttacgcaagt
     1321 attgacagat aatgtactcg acaaaatgga aaaagagaac ccaatagctg ataatctgcg
     1381 ttatgaatta gttgaaaatt ctgataatga atttgttgct accaaatttt cggataattt
     1441 tctgtcgacc tttgttgagg gcattaaatc aggatattat cgctatcaag caaatctatg
     1501 ggggatttct tttggattga ttgctttagc ggtagcgtat gtttttagtg ctttcattat
     1561 aattacagca atactcgagc tagcgtttaa acgcgtactt ggagttttag tatttgctac
     1621 tgacttggaa acaggccaac gatcaaagca agttttgtca gatattttac agtgttattt
     1681 gacagtcgga tttcaagggt tcggattgtc tatgtttgca atgtttgtta attatctcgg
     1741 aacaggtagc ggttcttctg taaatccttt tataaaagca gtagcgtacg tctgcgctgt
     1801 tattgtgctg ataaagggta gtggaacagt catgcgttac tttggcgtgg atattggttt
     1861 aaaagaaggc tatggccagc ttgcttcagc atttggcatg ggagctatgc tgtttcgtaa
     1921 aggaacaggt ggtatgaacc gatctaaagg tgggaaaaat agtggtggaa acgatcaagc
     1981 ggaaggggaa gaacgaaaac ctgaacgcaa ctttggtcaa tcactttctc aaaaagctaa
     2041 taaaacgggg aaaacacttg ggtatgctcg tgaacgagga ttatcaggct tagcttcgga
     2101 tggagctaca atggccagtg aaagagcaac aaaaccattt aagagtatgc gtgatatggc
     2161 taatgatacc aagaataaat tcaaagaagg tttagatgat ggtaccgtgt acgctattaa
     2221 taaaaatagc aaacctatag tagctaaaaa caaagaagat gaaactggaa aatatgctga
     2281 ttctatgcca acacgtcttt cggatagaaa agatggcgct aaagttgaaa acgctgatag
     2341 aattatgtct agttctgaac gtatgcgtga agcaatgaaa aataatgctg aatcaaatat
     2401 taatcctgca agtgcaattc agcaaaaagt taaacaagat attgaagaac gtaaaaatgc
     2461 tatgcacggt caagctaagt ctgctgaagt attaattaat cagaagcgac aggaagcaaa
     2521 atacacgcct gaagcaatga atcgtgagga aatgttaagg aaacgtgtag aagggcgtac
     2581 aggcgctaat atggacgaaa aagaagcgtt gactaaagaa agaatccaag aagctaaaaa
     2641 ttcaaatacc ggattagaaa aatcagtcaa agaaaatcta caaaattcta caagtagtca
     2701 agataggcgc tcagtagatg ttcgagaaaa cttacaaaat actacaagta gtcaaaatgg
     2761 aaatcgaaca gtatatgtac gagaaaatgt acaaagtagt gggacaagcc aaccgagaac
     2821 ggtagatgta cgagaaaact tgcagaatac tacaagtagt caaaatggaa atcgaacagt
     2881 agatgtacga gaaaatgtac aaagtagtgc gtcaaatcaa ccaagaacgg tagaagtacg
     2941 tgaaacaaga gctgaagcaa gtcagggaga taagacgcaa aaaataaata ttatcgagga
     3001 acgaaaagaa aggcctaacg aaaaagtgat tgtagaacaa gaagtgaagg agaagaaacg
     3061 tccacgattt acttatgaaa ataatccact gtttcaggat atttctgata aaccaaatcc
     3121 gctgtttgat agtctattta aaaaataaag aggtgattgt catgcaatta attctacaga
     3181 ttagtacgca ggatttctta gaacttgccg aattatgcga ctcagtaaag aaagcgacaa
     3241 atgaaaaaat cacggtcaca aattatattg aactgatgat tcaagaagaa ttaatgatga
     3301 aaaatgaaat agagagcaat ttcaaatgtt gctctctaga aaaaatgtga ggggataatc
     3361 aatggatgat aaacaaaaaa tgtacgaaaa aaatcgcaaa catagtgagg ccttaaattt
     3421 tcgtgtcgat aaaaagactt atcttgaact gacattgatt gcaaaacaaa gaggaatttc
     3481 tagagcagat gtttgtcgtg agcggttaaa gggactcaaa tatgaaccgc cgttattggc
     3541 tgaagatgat agggtagctc taatcaaaca gttaaatcaa attggtaaca atttgaatca
     3601 agcggtaaaa gcattaaata tcattgctaa atattttaga gaacaatctg aaaataagaa
     3661 agaaaagagc ctgcatttta gttttgacgt tagccgttac aacattttta aagatgaact
     3721 tgcgaatagc atagcaaaaa aaatgatgat tagttatcca gatgacattt ttttggagtc
     3781 caatcggagt acatcttctt ttctcaaact gtcagaagta gaaaaagatt attttcaata
     3841 ttggttagaa aagcaaacta gacccgatac gaaagaagag tattcccaaa aagttgaccg
     3901 gaatttaatt cactcgattc aaattattgc aagtttaaaa gatgaggtga aacaactttg
     3961 ggagctacta taacatcgaa aggacgcaag aaaaatttat cctcgctatt acaatatgca
     4021 gctaatcctt caaaaaaaga aaaatgcgtt gcagtctcag gaataaattg tagtgataat
     4081 agtgaggaag cattgcaaga aatgaattta gtaagaacca ggatgaataa atctgaagat
     4141 atagcacaag catttcatgt tatccattcg tttgataaga atatttctaa agagttaagt
     4201 attgagaaaa tgcacgaaat agctgtcgaa tttgcagaaa aagcttttcc aaattcgcaa
     4261 attattgttg caagtcacaa tgataaagat cattttcata gtcatcttgt tattaataat
     4321 attgatatgg agacctgtaa gaggttgcgt attgacccta aagatttaga acgaatgcga
     4381 aaaattaacg atgaggtgct tgttgaaaat ggactcgaac ctgtagacaa tcagtactat
     4441 gaaaatttag aaaagagaac gaatctctat tcaaacgatc caagtaaact gaaaaatgga
     4501 aaaattaatt atcaagagtt tgtacaaaat gcgattgaag ctacgttgga gaattgtgaa
     4561 gtaaattcat ttgtgcgttt ttcggatcat ttagcaaaag aatacaacat cgaagtttat
     4621 caattttcaa aaacaaataa caaacttggc tatgttcttt acaaacaaga tgtaaatttc
     4681 tatgagcgta gagatgagct aattacttta ggaagagaaa aacaatctag aaaagaaaaa
     4741 agtcagtatg tcgaacgtac ttttagtgca agaaagttag ggaaaaaata ttcatttgaa
     4801 tctgtttttg aacgacttga aaagaattta gaagaaacac atatgaataa ttttgttgat
     4861 ctagatacgg aacaaattat agcgttagct agtggagttg attataaaac tggggagtta
     4921 aatgtaccgt ttgtggaatt agattccatt gcacaaaagc agtttagtga tcatgagggt
     4981 gctaaaaaaa tcattaaata tgatgatttc ttgcgagaag tagacaggca ttatgcaaac
     5041 acaatatctt tagaagatga tcaagaatac acgattaaac ttcatactga tgtgaatcct
     5101 agaactttac gtagtactaa aagtaaaaca attttagtta aaaagacaga tcagaaagaa
     5161 ttatttctac cagctgaggg ctttaaaaat tgttctcata caccttctgc taaacagatt
     5221 cactatcaaa tcagtcgcat aatgaatgac gatgtgaaaa tgtatcgtgc taaaaaagat
     5281 tacgagttca cgcaacaaat gattgaaaat cagcaaagga gacaacgaga ttttgagatg
     5341 gatttctaaa ggaattagcc agtagacttt ccagtttccc atcgatacgg agggaattgg
     5401 aaagaggcgg ttttgagggc atacgccagc tacccatcta cttgccagtc aatccgccct
     5461 tgcttacgca gggctacttt ctggcaagtt agatggggaa aacctctgag ggagcaagct
     5521 ccctacagac tccctaaata aaaaaagata aggagtaaaa acacatgtct gatccgtata
     5581 ttaaagtgta ttgttcaaaa gaaactaaaa caatttttga cagtttttac gaacagaaca
     5641 aagggatttt cggaacaaaa gccaatatgt ttcgtgtgat ggtatcaaat ttaccagtgt
     5701 tagctaacca atcaaatgta aaattaagta atcctgaaag caaaggaatt gaacaaaaaa
     5761 tttctgaaat agaatcaatt gtggtgggaa agttggatga aattgatcaa aagttatcgt
     5821 attatctaaa aaacaaatgc aaaacggagg aggggaaaga tgtttaaagt gtattttccg
     5881 tttcagtggc tctttaaagg aattctttta ttgctactag gattgattct ttttggaagc
     5941 tatctaacaa ttaaagccag atattttcca gaagaaaaac cagtaaaaat tgtaactaaa
     6001 gatttaaaaa tgggtgaaac ttatcaggca gatggggtcg aaataacgtt taaaaaggtt
     6061 gaattgaccg aaaaaagaaa tgaatttgcg caacatgatc gtgtattaca agtgacctat
     6121 acaatgaaaa atgatacaga aaatccgata cgtccgcaac aatggtcgat tgaaaataaa
     6181 aggctcttcg tcaaatcgtg ttggtgaaga caaatttgaa taaaacttag tccgatatgc
     6241 ggcgcgttct gtgcgccgca t
//
LOCUS       DACCCS010000104         6073 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.112, whole genome shotgun sequence.
ACCESSION   DACCCS010000104 DACCCS010000000
VERSION     DACCCS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.112"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IVK82_002673"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IVK82_002673"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP6393680.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IVK82_002674"
     CDS             complement(1172..1867)
                     /locus_tag="IVK82_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP6393681.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IVK82_002675"
     CDS             complement(1880..2596)
                     /locus_tag="IVK82_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP6393682.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IVK82_002676"
     CDS             2926..3534
                     /locus_tag="IVK82_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP6393683.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IVK82_002677"
     CDS             3859..4182
                     /locus_tag="IVK82_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP6393684.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IVK82_002678"
     CDS             4364..5734
                     /locus_tag="IVK82_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP6393685.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..>6073)
                     /locus_tag="IVK82_002679"
     CDS             complement(5864..>6073)
                     /locus_tag="IVK82_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP6393686.1"
                     /translation="DDFQRYFHVEIPEFEETNFTTLAGFASSRYKEIKAGTIIEIASF
                     RFTVLEYQHAHIDYFKVESTERKTE"
BASE COUNT         1727 a         1196 c         1132 g         2018 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atc
//
LOCUS       DACCCS010000105         5947 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.113, whole genome shotgun sequence.
ACCESSION   DACCCS010000105 DACCCS010000000
VERSION     DACCCS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.113"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(76..1170)
                     /locus_tag="IVK82_002680"
     CDS             complement(76..1170)
                     /locus_tag="IVK82_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP6393687.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="IVK82_002681"
     CDS             1359..2120
                     /locus_tag="IVK82_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6393688.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IVK82_002682"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IVK82_002682"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP6393689.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="IVK82_002683"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="IVK82_002683"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP6393690.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4773)
                     /locus_tag="IVK82_002684"
     CDS             complement(3760..4773)
                     /locus_tag="IVK82_002684"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP6393691.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4938..5858
                     /locus_tag="IVK82_002685"
     CDS             4938..5858
                     /locus_tag="IVK82_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP6393692.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1815 a         1188 c         1065 g         1879 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtcccacg ctctaaacac
     5941 gtataaa
//
LOCUS       DACCCS010000106         5907 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.114, whole genome shotgun sequence.
ACCESSION   DACCCS010000106 DACCCS010000000
VERSION     DACCCS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.114"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(95..667)
                     /locus_tag="IVK82_002686"
     CDS             complement(95..667)
                     /locus_tag="IVK82_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6393693.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(674..1336)
                     /gene="rpe"
                     /locus_tag="IVK82_002687"
     CDS             complement(674..1336)
                     /gene="rpe"
                     /locus_tag="IVK82_002687"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP6393694.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1352..1867)
                     /locus_tag="IVK82_002688"
     CDS             complement(1352..1867)
                     /locus_tag="IVK82_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP6393695.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1869..2153)
                     /locus_tag="IVK82_002689"
     CDS             complement(1869..2153)
                     /locus_tag="IVK82_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6393696.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2187..3515)
                     /locus_tag="IVK82_002690"
     CDS             complement(2187..3515)
                     /locus_tag="IVK82_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP6393697.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3529..3984)
                     /locus_tag="IVK82_002691"
     CDS             complement(3529..3984)
                     /locus_tag="IVK82_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6393698.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3987..>5907)
                     /locus_tag="IVK82_002692"
     CDS             complement(3987..>5907)
                     /locus_tag="IVK82_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP6393699.1"
                     /translation="KLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLEDYVVNYQVTI
                     ERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHL
                     SQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAIKQLVFYYAN
                     DMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLL
                     KGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVT
                     NNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQN
                     PLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNII
                     IICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIP
                     LVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTG
                     VQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIE
                     SNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQKEMVDRLLQV
                     HDSEKLLRIFQQKGE"
BASE COUNT         1919 a         1147 c          836 g         2005 t
ORIGIN      
        1 aattattaag ttatgtctaa tcaaataaaa aaaccattct catatttgct gaataattag
       61 catttagaga atggtttttt atctttaaaa tctactactc aaaatctgca tgtcgagcaa
      121 acatcgtttc tgagctttca cctgttttat ccattaaaga taacactaaa ctatcaaagg
      181 tgagaaatgc taattgttca aaacttgaac ccataggttg tgaaaaagta tcttcttctc
      241 gttgattttc atctttggta atacctggta atacaagaac agaatctgct aattgaccaa
      301 tcgacgagtc ttttttcatg gtaactaaag caatgcttac accactttga tttgctttgg
      361 ccgctaaatt ttttaaacta cctgtttcac ctgagccaga acagataatt aataaatctc
      421 caggttgcga atgtggtgaa ctaatttcac cgacaaggct gactgaaaaa cctaaatgca
      481 ttaagcgatt cgtaaatgct tgcatggcaa ttccagagcg tcctgcacca gctagaaaga
      541 tgtgtttagc ttgaacaatt ttctcactaa attgttctaa ctctacagaa ttaatacgtt
      601 ttgagttatt ttgtaattct aagataatcg cttctaaata gtttttattt tggtctttat
      661 tggtcatgat cttttacttc acttcctcta atgattgatt tagtttatca atttgttctt
      721 gcggggtatt tttcccattg aagatgaaac cgcctgatac aaaaatatcc gcccctgctt
      781 tagaacaaac tttaatcgtt tgattatcaa tcttgccatc tacttcaata tcatacgtat
      841 aatcattttg tttgcgtatt acatctaact gttcgatttt aactactgtc tcttgaataa
      901 aatgcttatt ctctgttcct ggattgattg tcattactaa tacttgatcg actaaagata
      961 atactggttc aataaaactg actggtgttg caggattaaa agtaatccct acttttgctc
     1021 ctgcttcctt gactaactga atgaccttca ttgcgtgttt ggtagcttca atgtggaagg
     1081 taacaatatc tgctcctgct tctacaaatc ttgtgacaat tttttctggt gtatcaatca
     1141 tcaaatgaac atcaaacaca acgtctgtta gtggacgaat atctttaata acacttggtc
     1201 caaaagccat atctggtaca aaacagccat ccattacatc aatatgcaaa taatcaacat
     1261 tcttttcttt taagatttca aataactctt taacttctgc gataggtgca tttaacaatg
     1321 atggagcaat tgtcattctc tattcccccg tttattctcc cataatcatg agattgattg
     1381 ttctagttcg ctctcttagt tgatcccaat cgacaaaata gacacggccc atttctccta
     1441 ataaaagttt accttcttga acaattaaag tcattgagtt ggttccataa atagatgaac
     1501 gtaaatgtgc atcggtattt agcatgaccc atttttctgc agggtaattg ggatcagata
     1561 atcttgttcc aaaatcaatg tgttttggcc ccggactgtt gtattgattt tcactcgtca
     1621 tttttggaat aatcgtttcc attacattgt tgatgtctac atgtaaataa tcatcaccaa
     1681 aaaagtttgt gtcatgcatc gtttcttcaa aaaagagtga gcaagttgta tgcgggctcg
     1741 aaattactaa cattccattc tcaatacctg ctgtatcaac tgtatctaaa agttcttttc
     1801 gaatatcatg atacgaagga cgccctgaaa ctgtttcaat agtcaaagtg tcatgatgaa
     1861 aacgcatatt aaacacctaa tctttctttt acatctgcat aaatggcatc tgtaccaata
     1921 cctgttaata agcccaagcc attcgttact ttagctttaa ttacatcagg gacaattgtt
     1981 gtacttacta cagcatcata ttcatcaatt ttatttagct catcagcaat tttagattga
     2041 taaattttca cttgatctgt taatccttct tcttccaacc attttgataa ttttgctact
     2101 gcaattgtag aagtagcatg acctgctcca caaataacta ataatttttt catattgatc
     2161 tcttctttct tattttaatt ttatgattaa gctttcttca ctttgttcat gtagatagaa
     2221 gcacctacta aaacaactcc taatccacct attccaatta caccaaagta agatgctaat
     2281 aaaacaaaca accctactgg ccataatcca cataacaaca tagttactaa tcctgctgtc
     2341 cccacatcga aacttgctaa tgtaaacaca tcagtgataa ttggtgccaa ccatgttgcc
     2401 atatataatg ttgtaacaac atacaacgaa catcctacta atgatctcac aatatttcct
     2461 ttaaaataag gaatcattaa acagatggcg aaagcaaaga atgctaaatc tccaaaaggt
     2521 aatactttat ttcctggaag aattagtgct agcaataatg agattggtac aagcaataga
     2581 gaagttgaca taatagttgg atgacccaca gttaacgctg catccattcc aatgtttact
     2641 tggcgaccat ttaacttttt attcgtaaat tctttcgccg cttctgcaat tggcattaaa
     2701 ccttccataa acattgctac cattttaggc ataatcttca tcactgccgc agtcataatt
     2761 cctaagtttc caattcctgc tacgtcatag cctgccataa tacccactac aataccgata
     2821 attaggccaa taacaactgt gtcacctaaa acaccgaaac gttttgttaa tgtttctgca
     2881 tctgcatcta ttttattaaa tccaggaata cgatcaaaaa cccaatttac tggtttggca
     2941 aatagtactc ctgataatcc catcatatgt gtgatagaga ttcctggata gccgaaaaat
     3001 tcgctaacag ttttttgtgt acgatctgct aagaaccatt gtaaaattac tgcgacaatc
     3061 gcgccaatta agccaaacca aaagtttccc gtaacagatt gagtaatcat acctaggaaa
     3121 attgcgaacc aaaagttcca aatatcaaca ttcaatgttt ttgtccaacc caaaaccaca
     3181 aatatgaagt taagaattaa tgaaattgga atcattaaaa ttcccaatga tgtagagtaa
     3241 gccaaaggtc caccaacacc tacgccaata tctaggatat tcaggccacc accgtattgt
     3301 tcccccattt gttgaattgc tgttcctaaa ccatctgata ataaacttaa aactaatcct
     3361 aaaccaatag aaccgatacc aacagtaacg ccggaaacaa atgctttacc aggttttaat
     3421 ccaaataata tcccgattac aaaaataatc agtggtaaaa agacgtttga ccctaatcca
     3481 ataaaccatt caaaggcatt taatacaata tccatctctc tattcctcct aataaactcc
     3541 tgcagaatgt actaactcaa taaatgcttc tttcgtggta catgcaagta attcatttac
     3601 tttttcttca ttttgaaata ggctaactaa gttttgtagt gtatcaattt gttcatgagg
     3661 ttgtgacatt gccaacataa agactaaacg tacaggtatt tcatgagatt tatctgtcat
     3721 atctgaaaat accagtggtt cctttaatga ggcaaaacag atttgagagc tatttacatg
     3781 tgcggaatct gtatgtggga tagcaaaccc aatttgattt acttctaatc cagttggaaa
     3841 ttcttgttca cggttggtga taccttctaa gaaactatct ttcacgcaac cagcgttttt
     3901 taattgttca gacattactt gaaacaattc tttttgatct ttcacttcta attgaaataa
     3961 acataattct ttatctagat acatttttat tctccttttt gttgaaaaat tcttagtaat
     4021 ttttcgctat catgaacttg taacaagcga tcgaccattt ctttttgccc aataaattgg
     4081 taaagttctt catatactcc tctaatctca gatacatctt cttcagctaa actaaccata
     4141 atgactaatt tgacttttcg atcgccccaa ttaatcgcat tttttaatgc tacaataaaa
     4201 atgttggatt caatgaccat tgatggattc gcatgcggta aagcaacacc actttctaaa
     4261 ttagtattcc ccattttctc tctagagaag atagaagcac gaaactcttc agtaactaat
     4321 gaattggctt ctagttcgga aatcatcaaa tccaaacatt cttctttgct ttccacctgt
     4381 actcctgttt ttactaaaga tgattgaaca aatttagata tggtgggaac tttaaccgct
     4441 ttaggaatca gttcattttg aataaatcgc gaaggttctt ctattaaata tttagtataa
     4501 gcttccataa tattcatata atctgtttta cttaccaatg gagataccct tactaagggt
     4561 ataccacctg catcaaagga aagagaggaa ataatcaaat caacttcccc aatttcagct
     4621 aaatgaaact tatctaaagt tgatatttct acattatctt ttgctggtaa aaaccgtcga
     4681 actttattgt aaattaattg cgcagaagaa ataccgtatt ggcaaataat gatgatatta
     4741 tttgctttgg actgatcttc aatagcaatc tgaaaatgaa tcaaaattaa ggaaacttca
     4801 tcgtccgtta aagtcacatc aaattcttct tcaaggatgg acatggcgta ccatactatg
     4861 ctaaagagtt ctgaatattg agatttgata ttttctaata acggattttg aattttaata
     4921 ccttttttta aacgaaggac cattgctggc acatgataca ataaggattg atataatcga
     4981 tcattttgag tcaaatcaat tttttcaatc tcactcattc tttcaatcaa tttatgaacg
     5041 ctcatagcgt actcttcatt gctggttttt aaattatttg tcacacgatg tgcaaatagt
     5101 tgacgacata gatattcttt atctgcgtca taaaatggaa gattcaattt tgccttcaat
     5161 tcttcaatta aaccatttgc cacaatgtac gtttccatat agcgaatgct attgaagagg
     5221 aattcttctt caacagttaa atgatgacct ttcagtaaac ggttacattg gataactaaa
     5281 gtagaaatca atgaccgaac atagtaatca gaaacctttt cagtcagttc actgtaatca
     5341 ttgaatagca aatcatgaat gacactaata acttttgaat caaacaacat atttaaaact
     5401 tgaaaaaatg tttttgattc catgtcattc gcataataga atacaagttg cttgattgct
     5461 cgttgaattg cttgttcatt accagtaaaa ctaattcctt ctgcagatga ctgcaactta
     5521 acttctccac tgccaataat tttattgata attctcaggt cattgtataa cgatgtttta
     5581 ctaaccataa aactttgaga caaatgagtt aaagaaacag gcttatcacc aaacaaaact
     5641 tctttaatga tctcgacacg tcttttttct ggagaataaa tatctttttc aagataattt
     5701 tctaccgatg aaatcaaatg ttcaacgttt ttcctatcac ctatgagttt gatacccaaa
     5761 gaaggtaatc gctcaattgt tacttggtaa ttaactacat aatcttccaa caattcaata
     5821 tcttgataaa tggtcttggt cgacactgcc aatttatcac taaaaaaaga agcgggttta
     5881 aagtcttctt gtcgacttaa taatttt
//
LOCUS       DACCCS010000107         5876 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.115, whole genome shotgun sequence.
ACCESSION   DACCCS010000107 DACCCS010000000
VERSION     DACCCS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.115"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            640..1374
                     /locus_tag="IVK82_002693"
     CDS             640..1374
                     /locus_tag="IVK82_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6393700.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1390..2559
                     /locus_tag="IVK82_002694"
     CDS             1390..2559
                     /locus_tag="IVK82_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6393701.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2575..3570
                     /gene="lacD"
                     /locus_tag="IVK82_002695"
     CDS             2575..3570
                     /gene="lacD"
                     /locus_tag="IVK82_002695"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP6393702.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3649..4575
                     /locus_tag="IVK82_002696"
     CDS             3649..4575
                     /locus_tag="IVK82_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP6393703.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4594..5844
                     /locus_tag="IVK82_002697"
     CDS             4594..5844
                     /locus_tag="IVK82_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6393704.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1967 a          955 c         1203 g         1751 t
ORIGIN      
        1 tttgaaaaat gctatttcga ggtagaagga tattatataa gcacggttga ctaaacgaag
       61 tgataataaa aaaagttatt caagaataag aaaaattatg atacgaattc agataaactg
      121 gagaatacag agacctttaa aagggataag aaacaacata aattcctctt aagagagaac
      181 gaaaggacaa agccagcaac ttgatacacg agctagtatt agaggaattt attctcatag
      241 actccctttt gaatacgttt caactttaat tttcatgttc ataaatgatt tgaaaccaaa
      301 gcaaatgcgt tgcacttttc ctccgtcttc cgaaaatcgt tcaattcact ggtaagtagt
      361 catttgatag tttgaggcaa attgttgatt gatgaagggt aggagctaaa aaaagatacc
      421 agttgaagag agaaaaagaa aaaaatagag ggtgttcttg aatgaaaaaa gttggaaaat
      481 tatttttcat ttccttttat tcctattaaa gtcagtgatg tcaatcatat taacaagact
      541 aaaaatgaaa ataaaaaaaa gattgttctt agtattgact cataaaaaat accactacta
      601 tacttacatt gtggttatag ccagttagga gagacatcga tgcaaaatac ctttcaatca
      661 aaatcacttt attatcagct ggtagaaata ttgaaacaac gaatcgaaaa cagtatggtc
      721 gctcatgaca agctgccatc agaaagagag ttgaccagtt tttatggcgt tagtcgcacg
      781 acggtgcgac tagcattgca agaactggaa aaaatgggct acatttatcg acaacatgga
      841 aaaggaacct ttgtttcgga attaaggaca aatgcgactg atctagctgg cgcatatagt
      901 tttaccgaac aaatgcgggc aatgggcaaa catcctgaaa ccaagatatt ggccttcaag
      961 aagattgaag caactaaata tttagcgagt catctgaatg ttcaactagg tgagccttta
     1021 tttcaattag accgattgcg cttagctgat gatttgccaa tgatgatcga gaaatcctat
     1081 ttgccagtga agctatttat gtccctttct caagaaattc tatttctgaa acctttatat
     1141 aatttgtttt cagagaattt caatcaaatc gtacggttgg ctgacgagga attttatgcc
     1201 agcattgtcc agaaagaaga tagtgagttg ctaaatattc cagaaggatc accagtgctc
     1261 catttagttc gctcaaccta taatctcgag aacgaagtga ttgaatttac attaagtgta
     1321 gcgcgggctg ataaatttca ttataagatc aggcatctgc gttccactaa ctgaattgga
     1381 ggaaagaaaa tgtttatgtt ctcaaaagaa gaactacgaa agctaggagc agaaaatact
     1441 acccgagaaa ttcgacagca accggaactg tggcatgaga cgtttgaccg ttatcaagaa
     1501 acagttgcag atttagatcg tttgtttacg gatgcgaaac aacatgctgg agagcgacca
     1561 atccgagtca tttttacagg ggccggaacc tcgcaatatg tgggagatac actaattccg
     1621 tttcttacta gtcaaggaaa tacgaaccag tttatttttc aaagtattgg ttcgaccgat
     1681 atcgttgcta cgccaaaaga atacctgatt cctgatgaga caacgatctt ggtctcattt
     1741 gcccgtagtg gaaatagtcc agaaagtcta gccgcagtta agatggccaa tcagctggtc
     1801 aacaagatct atcatatcgt gatcacctgt gcaccagacg gattattggc tacacaaatg
     1861 cctacaaaaa aagaaaatct agttttacta atgccaaaca aagccaatga cgctggtttt
     1921 gctatgacaa gcagtttcag ctgtatgacg ctgactgcgc tacttatttt tgatcaagca
     1981 agcaatcagc aaaaagaaag atatgttcat caattaagcc gactaggaga agaagtcatc
     2041 caacgagaat cagagatcca agggtatctg gcatcagact ttcatcgcat catttactta
     2101 ggttcgggaa gcttaggcgg attgactaga gaagcgcaat taaaagttct cgagttaact
     2161 gctggaaaaa tcgcaaccgt atttgataca tctttaggat tcagacatgg tcccaaatct
     2221 tttgtcaatg agcaaacatt ggttttcctg tttgtcagca atgactcata tacgcagaaa
     2281 tacgatgtag acatgctgga agaaatcaaa aatgacggta ttgcccaacg cgttgttgga
     2341 attggtcaaa agggttcatt gtttagtggt gattcctttg aattagttgc agattcgtta
     2401 ctgccggatg gctatcttgc tttagcagat atcatggtag cccaaacgat cgcattactt
     2461 tgttcggtga aggttggtaa tacaccagat acaccatcgc caagtggaac agtcaatcgt
     2521 gtggtcaaag gcgtaacgat ttatccatat gaaaaatagg aaagagtgtg aagcatgtta
     2581 gaatggtcgg aaaataaaag acaagcaatg gaacgtctat cgacgccaga aggcattatt
     2641 accgctttgg cgatcgatca gcgaggcgcg ctgaagaaaa tgatgtcggc tttagggatg
     2701 gaagcgacaa aggaaaaaat tgagcagttt aaaacactaa tttcccaaga attgactcct
     2761 tatgcctcag caattttatt ggatcctgaa tacggactgc ctgctgcaca gaagcgtgca
     2821 gcagcgagcg gtctgctgtt ggcatatgaa aaaactggtt atgatcaaac cgtacccggc
     2881 cgcttgccgg atctgctgtc ggactgttca gttctgcgat tgaaagagtt aggtgcggat
     2941 gcggtgaaat ttttactgta ttacgattat gaagaagcag aggaaatcaa tcggcaaaag
     3001 caagtttttg ttgaacgaat tggaagcgaa tgcatggctg aaggactgcc attttattta
     3061 gagttattgt catatgatgc taaaaatgta gatacgaaat cgctttccta tgccaaagca
     3121 aaaccgcgca aagtgatcga aatgatgaaa gaattctcga aaacccagta taaagtagat
     3181 gtcttaaagg ttgaagtacc agtaaacatg tcatttgttg aaggaaatgc aacaggggag
     3241 atcgcctata cacgtcaaga agctgccgct tattttaaag aacaaagcga tgcaacagat
     3301 ttgccgtata tcttcttaag tgctggggta tcagcggcat tattcatgga gacattgcgt
     3361 tttgccaaac atgctggggc acgattcaac ggtgtactct gtggcagagc aacttggaaa
     3421 gatgcagtcg ctcctttcgt tgaaaaagga gaagcggcaa ctttagaatg gctgacagaa
     3481 caagggacac aaaacattcg tcaattgaat gaagtgataa gagaaactgc catcccatgg
     3541 tatgagaaag taagtgaaag cacttgttaa tgtttctaat atattagtag tgataagaga
     3601 tatgtgtgat tataatttat cattattgaa actgggagga tattttatat gaataaaaga
     3661 ttgttagatt attcagcttc agattttttg aaagctaagc cgatggaatt aaagcaggca
     3721 attttggctt ctgaaggtag aacaattatg gcagaaaatg tgccaagtag ctgtccttac
     3781 atgggaaaat cagtgacaaa tgcagaattg gagagagctg ctggagctga tttaattttg
     3841 tttaatgcac tagatctttt taatcctcaa atttcaggtg tacctttaac tctaaaagag
     3901 aatccaataa tctggattaa aaaagcttta ggaagagcta ttggtgtaaa tttagaacct
     3961 gtagacaaag aagttttgat gagtgaaaat cgaactgaaa tttcaagtgg tagaatcgtt
     4021 tctcctgaga ctttaaaaaa ggcaaatgaa ttaggcttta attttatttg tttaactgga
     4081 aatccaggaa caggagtatc taatagaact attgagaaag caataacatt atccaaatca
     4141 atttttaatg gattaattat cgctggaaaa atgcatggtg ctggtgttga tgaaccaatt
     4201 atgaacttag atatagcaaa aagatttatt aaaacaggtg tagatatttt attagttcct
     4261 gctccataca ctgttccgta tttcatggaa agtgatttgc gaggaatcgt tgattatata
     4321 aaagatttta atagaaataa atctattgag gaaaaagttc tagtactaac ggctaatgga
     4381 acaagccaag actcttctga ccaagaaact attaaaaaaa ttgcattagc atcaaaagca
     4441 tgtggagctg atattcaaca cataggggat tctttaaatg gtatttgtct tccagaaaat
     4501 atttttgctt tgggtcaagc aattcgggga tatcgtcatc aaattattat gttaggaaaa
     4561 tctaattatc gttaattaag gaggaaatga aagatgatcg acaaagttca aaaaatagct
     4621 gcaaaaatta gtaatcaaag acatctacta gctttaagag acagcatggc tgcagttatg
     4681 ccattaatca ttattggttc cttttttatg ttattagcaa atttcccgat agaaggattt
     4741 acaaaatggt tatctgaaat tggagtacaa tcctttttga acaaagcatc agattcaaca
     4801 tttggtatta ttggattagc tgttacctac tctttgggac acaatctagc aaagacttat
     4861 ggtgtagact cattatcagc aggattatta tctttgagtg gatatgtttt acttacacct
     4921 ttattggatg cagatggttc tataggattt ccaacaaaat actttggtac ctcgggtctt
     4981 tttgttggta ttattgtagc acttgtatct acagaaattt tccggtggtt tgtacttaaa
     5041 aatattacaa ttaaaatgcc ggcaagcgtt ccaccaaatg taagccgagc tttcgttgct
     5101 attattcctg gcttttttgt cgttctacta tggtttattg ttgttgtaat ttgttataaa
     5161 cttggaattg aaaatgttca tgctcttatt gcagatacac tcgcaaaacc actatcttta
     5221 ctgacgaaaa ctttaccagg aattatattg gttatactta ttcaatgttt cttttggatg
     5281 tttggtattc atggtgcaca ggtaacgggg ccaataattg aaccgttatt attgcaaaac
     5341 tctgatgtaa atagaattgc ttatcaagct ggaaaagaac ttcctaacat tataacttat
     5401 gaattcttat ataactttgt tttttctggt ggtgcaggtt gtttatttgc acttgccatt
     5461 ttaacatttt tcttttcaaa gagtcaggaa aataaaactt taggtaagtt atcaatcgct
     5521 ccagtaagtt ttcaagtagc agagcccctt ctatttgggt ttccaactat attaaatata
     5581 aaaatgttaa ttccatttgt aactgcacca gtagtaacaa cattaattac ttatttttca
     5641 atgagtatgg gattagttgc aaaacctgta ggagcaacaa tcccatggac aactccaccg
     5701 ataattgccg gatttttagc ttctggtgga cgaataagtg gtgcagtgat tcaagtaata
     5761 acaattgcaa taaatgtttt gatatactat ccattcttta aattagatga taatgccaag
     5821 ctaaaaagcg aaaaaaatga ttagaactac ctatatgttt ctaactgtag ttaagt
//
LOCUS       DACCCS010000108         5712 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.116, whole genome shotgun sequence.
ACCESSION   DACCCS010000108 DACCCS010000000
VERSION     DACCCS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.116"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..70)
                     /locus_tag="IVK82_002698"
     CDS             complement(<1..70)
                     /locus_tag="IVK82_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP6393705.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEEL"
     gene            complement(206..1381)
                     /gene="mltG"
                     /locus_tag="IVK82_002699"
     CDS             complement(206..1381)
                     /gene="mltG"
                     /locus_tag="IVK82_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP6393706.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            1546..2706
                     /locus_tag="IVK82_002700"
     CDS             1546..2706
                     /locus_tag="IVK82_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP6393707.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(2746..3693)
                     /locus_tag="IVK82_002701"
     CDS             complement(2746..3693)
                     /locus_tag="IVK82_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP6393708.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(3826..5217)
                     /locus_tag="IVK82_002702"
     CDS             complement(3826..5217)
                     /locus_tag="IVK82_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAP6393709.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            5343..>5712
                     /gene="tagD"
                     /locus_tag="IVK82_002703"
     CDS             5343..>5712
                     /gene="tagD"
                     /locus_tag="IVK82_002703"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAP6393710.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTT"
BASE COUNT         1917 a         1061 c          860 g         1874 t
ORIGIN      
        1 ttagttcttc taattctttt tctaattttt ctttaccttc tagagtcatt ggataaactt
       61 tctctaccat gtgtaacgtc tccttttttc gataaaatac cgcaaaaggt aatcttttga
      121 ggaatatagt ccaaaaaaga ttaccacgct tttcttaaaa tgtaaatgtt tttctaaaaa
      181 tgatcaatga ctagcccctg tttttctaag aattatttac atatttttgc actagttcca
      241 tatgttcttc atacgtttta gcgaaataaa catttccagt gctcgtatct gctacgaaat
      301 aataatagtc attttctgct ggttctaata ctgccttgat tgccttttcg cttggattgt
      361 caaaaggacc aggcccatag ccggtattgg tatacaaatt gtatggtgaa tctacagctg
      421 tatcttcata cgtcaccaat tctttatgtt ctcctaatgc atataaaaca gagatatctg
      481 actgaagtgg catatttgcc ttgattcggt taaagaagac ttgtgcaata tttttacgat
      541 cattttcttt tacgccttct ttttcaacta gagaagccaa agttaaaact tcttgtacag
      601 tcaaattttt ctgtttgata gctgaataat agttttgcat gaccgtattt gtcttgttca
      661 ccatctcaat cacaagatcc ttaagcgaag tactcgtgta gtaatcataa gtagctggga
      721 ataagtagcc ttccaaacgg tattttgtgt tctcagcttt agaggcactt tccaacaatt
      781 ctgggaattt ttgatacaac tcgttaaaga aagtctcatc atttactaaa tctaaaaatt
      841 ctttcttgtc tttacctgtt accttcgcta cccgctcagc aatctgatcg atgtcgtagc
      901 cttctggaat ggcaattttc ccatctgcaa cttttgttgg ctcagatgtg ccgccttctt
      961 gcaactgctt gccaatctca tcaagagtca tattaggcgc taactgataa taaccagctt
     1021 gaaagccagt taagttttta aattttgtat aataattgaa aacaatccca cttttaataa
     1081 tattgtcttt ctccaaaatt tcgccaatct gcttattaga agagccgttt gggatttcca
     1141 cttggactaa ttggtcgtcc gatttatcta aaggcttcaa cccgctatct acataacgat
     1201 atacggtaaa cccgagaaat ccgccaataa tcaataatgt aagagcgatc actaaaatga
     1261 tcttcctcac gatcttatct tcttttttac gcagatttct ccgcttgtcg attatatctg
     1321 ctgtagctgc cttactggct tcttttttgt ctttgcttgg gtgttgtttg tttcctgaca
     1381 aaacaactcc tccttactat catcttcgta actctgttta ttatacatga aatttctact
     1441 gccgtgaatt attttaccaa atattatgtc aaaattgtaa gtggcaaatg ctctttttga
     1501 ggattacaat gaaaaatagt taaaaatgga tagaaaggat ttaatatggc aaaaggaaaa
     1561 catcaaaaaa aacagcagat ctctctttct tttctcgcag ttttgttgct ggctgtcagc
     1621 ttcttttcaa caggcatcat tacaggatgg caattaacaa aacaacctca accacaaatt
     1681 tataaaacgg aacccgcaca acttgcttcc aatttaacat ctaatgagca gatgcttgcc
     1741 agcaaaattg atcaattact gcaaaaatca gattatatcg gttcgattta tgtacgcaaa
     1801 aataatcgag tgatattaga aaaaggatat gggtacgcaa atctacgcat aaaagaatcg
     1861 aattctcctt ctctttacta tcaaatcggt tcgatccaaa aagcgatgac tgctttactt
     1921 atcctaaaac aaattcaatc tggcaagata acttttgaca caaagctatc tgaattttat
     1981 cctcagattc ctggaagtca gcaaatctca atcaaaaata tgctttacat gcgctctggt
     2041 cttcatcgaa ccgcttctcc aaaaaaacct atgtcagata gtgagatcat tcagtttgcc
     2101 cttcatcatt tgaaatttac tggttatgat acgtatcatt atgaaccatt aaattataca
     2161 ttgcttacag gggtattgat caaactaatg aatcagcctt atgaaactct tttaaaaaaa
     2221 gaaatcattc ggccactcca tctagaacat acagattttt atgaaaatgt aaaaaattcc
     2281 cctcagcatg ctgtatctta ccaaatgtct gcaactgacg attattcaaa agcattggtc
     2341 gagcctgaaa cagacatccg aaatgaatta ggcacaggaa atataagtat gtctgtttat
     2401 gacttgaaca aattctttac cagtgttctg tctggcgata ttataccaaa agaactactt
     2461 ttctcattgt ggcaaaataa tggtgataaa catccttaca gcggcggtgt ttacagtgga
     2521 aatgattata tccttgctca gggaaatatc aatcgattcc atgcagtagc ggcgatgaaa
     2581 aaagacacaa aagatgcaat cgtcatggaa agtaatatcc aatctgataa aaacatcaaa
     2641 ttgccggcca ctgatttaag aaatcagatt tacgaactga tggaaggtgt taatttaaaa
     2701 agctaagtcg agacaaaaaa accagcagga tgctggtttt ttgtttcaat cgatttctgt
     2761 ctttaaataa cgttccattc cgttcaatat atcaaatttg ggttgataac ctaaagaacg
     2821 gatctttgaa atatccgcta aagaatcacg aatatcccct gaacgctccg cttcatattt
     2881 aagcgctagc tctgttccta agatttgatc aattgaatga atcaattcaa gtagcgtagt
     2941 gctttttcct gtaccgacat tgaattgctg tcctaaagct ttttcttcct tagcaacaag
     3001 taaaagtgct tgtataacat cttctatata aacgaagtct ctagattggc taccatcacc
     3061 aaataaagta aattctgttt tttctcctgc caattgtttt ttgtatcggt ccactagaat
     3121 cgaaataact ccgctatatg gagagttagg attttgattt ggaccataaa cgttaaaaaa
     3181 tcggacagca ctagtcggaa catcatataa atgacagtaa tccaaaacat attgttctga
     3241 tgcaaactta tcgatggcat atggtgttaa aggacggatg accgattctt ctttttttgg
     3301 caacgttggt tcatcgccat aaactgcagc tgatgaacta aataccaatc gttttaaacc
     3361 agattgatat tttcgtacca attcaagcaa taaaagcgca ctgtcaaaat ttacacgatg
     3421 agtttctaga ggacgttcta cagaatctgc cacactagct acagcagcta aatgaaaaat
     3481 ataggcaaac tgatactgtt tcatgattct ttccatcaat tgaggatctg atacatcccc
     3541 ctcaatgaaa gtcacttgtt tctccatatc caagttttct ttttttccca ttgataaatc
     3601 gtcgactact acgattttgt tttcttttcc caaataattc gctagcgttg aaccgataaa
     3661 accagctcca ccagtaatca atatagtatc catcaaattc actccaatta tgcattctca
     3721 tacttaataa agttagttta ccatttttat attgtaaaaa ctataaaatt tgttgaagtt
     3781 gggataacta gttttctttt ctactttata tacctaaaaa tagtttcaat tagttactcc
     3841 ttcttcttta gatatagttt tttggataga acccagatat ccccaaaaaa taaatgaacc
     3901 tatcgtacta accaaaatga gttctgtgat aaataatgcc gaaactgcta ttgtaagaat
     3961 cattaaaata gcaaaaaagt gcttatcttt tactagattt ggttgtgtga aacaataacg
     4021 caaagtttta aagcattgga taaataagaa ggttaacaat gtcaatagac ctaatcctcc
     4081 tgtagcagcc ataactaaga aaataaaatt atgagcagtt aaagtatctt ttcccattaa
     4141 tgtatatggt agtttatcat gtgcgtattc aatatagtat cctggagaac cgccggatat
     4201 cggcttactc ttgaatattt caattgcact tttccaaagt ccaaacctta aattagaaac
     4261 atccccgcca tcagtgtcag ggcgttttaa atcaacttta ttgctgctag ttgttcctga
     4321 tgaatcagat atatcttttt ttatttctgt aatatgcgga acatgtgccc atgcttgagc
     4381 agttatatct gttacataat acatcgcaat tatacttagc aatcctacta ctaatgcagt
     4441 tagccatctt tttaaagtat tagttttaag aaaacgacta ctataacttg taaagaaact
     4501 aaaaacaaaa gttgctatta ataaacaaac taatgtggtg cgtgagcccg ataaagcaat
     4561 aaacgagaat tgaataaaaa tattaattgc aaaaaatatt ttaaatagaa gatttaaatg
     4621 cttgtaagtg accaaataat atatagcaat aatgatgaca attaatgata taaccgcacc
     4681 aaaatttgga tctgtatata caccaaataa acggttgcct aaccacccta aacgaactgg
     4741 gtaatactta tattttattg gaatttttat gccatattga atgacaaacc agtataatga
     4801 tactaaatta gctaaaaacc ataccaatga aagtatccaa gtaaatactt tgaaaaaaaa
     4861 gttactactg tagtctttgc caaactgata aatcataaag aaaaagatta attgccacat
     4921 gattaacttg atattttcgc tatatccata ttttaaattc attaaactgc ttactagcat
     4981 cgcgccaata aaagcaatta atagccaatc atatttttta agattttttt ctctaaatgc
     5041 aataattgca tctataatga tcaagacaac tcccgctatt ccagcaggcc aaaaaagtaa
     5101 agtatcatat ctagtaggta atagtaaata cgggcctatt gctcgtaaca ttagaataaa
     5161 accaaaataa ataaaatacc aacctttcac atattttaaa aatttttcat tcaacatttt
     5221 tcctccatcc acctatttct cataaacatt tcttatcctt tttaggataa actggttctt
     5281 tcaattctaa caaaaaaatg ctaaactaac aatgtatatt aatgaatttc gagaggtgaa
     5341 agatgaaaag agtaattact tacggtacct ttgatttgct tcactacggg catatcaatt
     5401 tactgaaacg agcaaaacaa tatggagatt atcttattgt tgccctttct acagatgaat
     5461 tcaattggaa tgcgaaacaa aaaaaatgtt attttagtta tgaaaaaaga aaacagttac
     5521 ttgaagctat tcgctacgtt gatttagtta ttcctgaaga aaattgggaa caaaaagtaa
     5581 aagatataaa agaataccat gcagattatt tcgtgatggg cgatgattgg gctggacaat
     5641 ttgatgaact tgaacaacta acagatgcac aagtaattta tttaccaaga acacctgaga
     5701 tctctacaac tc
//
LOCUS       DACCCS010000109         5664 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.117, whole genome shotgun sequence.
ACCESSION   DACCCS010000109 DACCCS010000000
VERSION     DACCCS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.117"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..194)
                     /locus_tag="IVK82_002704"
     CDS             complement(<1..194)
                     /locus_tag="IVK82_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393711.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFE"
     gene            complement(737..2887)
                     /locus_tag="IVK82_002705"
                     /pseudo
     CDS             complement(737..2887)
                     /locus_tag="IVK82_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3033..3608
                     /locus_tag="IVK82_002706"
     CDS             3033..3608
                     /locus_tag="IVK82_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6393712.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3822..4517
                     /gene="vanR"
                     /locus_tag="IVK82_002707"
     CDS             3822..4517
                     /gene="vanR"
                     /locus_tag="IVK82_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP6393713.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4495..5649
                     /locus_tag="IVK82_002708"
     CDS             4495..5649
                     /locus_tag="IVK82_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP6393714.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1770 a         1092 c         1073 g         1729 t
ORIGIN      
        1 tgttcaaaat ctaaaatcaa cacatcatgg ggagtgtgat aataaatatt ttcccttaat
       61 gtaccaacaa tggtaattcg gtcaattgac cattgcattt ttaagggggt gcacatagtt
      121 tgtgcattta accgcctgtt agaaatacgg ttaagttttt cccactcatt tacgattatg
      181 ccttttttac tcaaaaatag ctcctcctca acgaaaaaag agattcttgg aaagaatttc
      241 cagagttcac gtaactctaa aaatccacat ccaaaaatct ctagcttgaa gaaacctaaa
      301 aatattcgta taatgaactt gagttacgga actaggtgaa gtagtttccg taaaggtcga
      361 gagtgcgtca acactgctcg actatttttt tactcttttt tctcattttc tttgattata
      421 gcactcctct agaaaaaata aaaagacctc agggaatttc attccccgaa ccccttgagc
      481 ctcctcctgc cgaaatagaa tggcgctgcc agcatttatt tcgttcagct atcggctaca
      541 caatctaaca cgaccgttcc gctaccagtc gccttacagg ctccattttc gctcctctaa
      601 cgtgtttcac cgattgtgcc gataactaaa cgaaataaac gctaaaaccg ctcagaacgc
      661 aaaatatgcc attttagagc tattgtagaa aatatagttt gaaatgcaat tttcttcttt
      721 cagcttttta tttcccactt gtaaatcggt agatggtggc tccttttcca gttccataat
      781 gtggatttgc atctgcatgt agtgatgaaa cacctagctg cattctcata ccatctgacg
      841 aagatgttgt accgtcgccc caatagaaag gcaattgtaa tttatgatga aagtttacta
      901 atatggcttg ggctttattc atggcatctt catacatgcg ccattgagat acattggcta
      961 gttgcttata tgtaagtccg ggtgtggctt cggccatctt gctcaagcca atattcattc
     1021 ccattcctaa aagggcagcc atgataatga ttgtttcttc cttatctggt tttcgattat
     1081 tggaagcatg agtgaattgc tcatgaaatc ctgttatatg ggccacatcc atgagtaaat
     1141 cagttaattt tattcttggt agcatctgat aaaggcttgc actaaatttt tttgcttctt
     1201 ctggaacatc tttttctaag cgtgcaagtg atagctttcc tttttcaaga gaaaccccat
     1261 ctaacttatt ggaattggca gctaaccact ttaacctttc attaaagctg ctggttctct
     1321 ccgttatata atcttcgaat gataaactaa ctgataatct cgtattcccc ttcgattgat
     1381 tccatgtatc ttccgaaaac aaatattcct caaaatccct atattgtctg ctgccaacaa
     1441 tggaaacatc tcctgcccga acatgctccc gaagttctgt taaaacagcc atttcatagt
     1501 aatgacgatt aattgttgta ccatcatcct cgtataaatg tcttttccat cgttttgaaa
     1561 taaaatccac aggtgagtca tcaggcactt ttcgctttcc agattcgttc attcctcgga
     1621 taatctcaac agcttgtaaa agtggctcat ttgcctttgt agaatgaaat tccaatactc
     1681 ttaatagcgt tggcgtatat tttcttagtg aataaaaccg tttttgcagt aagtctaaat
     1741 aatcatagtc ggcaggacgt gcaagttcct gagcctcttc tactgaagag acaaaggtat
     1801 tccattcaat aaccgattct aaaaccttaa aaacgtctaa tttttcctct cttgctttaa
     1861 ttaatgcttg tccgatgttc gtaaagtgta taactttctc atttagcttt ttaccgtttt
     1921 gtttctggat ttcctcttga gccttacgac cttttgataa caaactaagt atttgcctat
     1981 catgaatttc aaacgcttta tccgttagct cctgagtaag ttgtaataaa tagatggtta
     2041 atatcgaata acgtttattt tcttgaaagt cacggaatgc atacggctcg tatcttgagc
     2101 ctaagcgaga cagctgcaac aggcggttac ggtgcaaatg actaatttgc actgtttcta
     2161 aatccattcc tcgtatgtat tcgagtcgtt ctattatttt tagaaaagtt tcgggtgaag
     2221 gatgacccgg tggctctttt aaccaaccca atatcgtttt attggattcg gatggatgct
     2281 gcgaggtaat aatcccttca agcttttctt tttgctcatt tgttagagat ttactaaccg
     2341 tattaaatag cttcttttca gccattgccc ttgcttccca caccattctt tcaagtgtag
     2401 tgatagcagg cagtataatt ttgttttttc ttagaaaatc tatgcattca tgcagtagat
     2461 gaatggcatc accattttcc aaagctaatt gatgaaggta cttaaatgtc attcgatatt
     2521 cactcagggt aaaagttaca aagtcgtatt cacttcgaat ttctttcaaa tgatcccaaa
     2581 gtgtattttc cctttgagga taatgatcaa gcgaggatgg actaacacca atctgtttcg
     2641 atatatattg tatgaccgaa tctgggatgc ttttgatatg agtgtatggc caaccgggat
     2701 accgaagaac agctaattga acagcaaatc ctaaacggtt ttcttccctc cttcgcttat
     2761 taactatttc taaatcccgt ttggaaaaag tgaagtaggt ccccagtatc cattcatctt
     2821 cagggatttg cataaaagcc tgtctctgtt ccggtgtaag caattctcta cctctcgcaa
     2881 ttttcattca gtatcattcc atttctgtat tttcaattta ttagttcaat tatatatcaa
     2941 tagagtgtac tctattgata caaatgtagt agactgataa aatcatagtt aagagcgtct
     3001 cataagactt gtctcaaaaa tgaggtgata ttttgcggaa aatcggttat attcgtgtca
     3061 gttcgactaa ccagaatcct tcaagacaat ttcagcagtt gaacgagatc ggaatggata
     3121 ttatatatga agagaaagtt tcaggagcaa caaaggatcg cgagcaactt caaaaagtgt
     3181 tagacgattt acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta
     3241 gtacacaaga tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat
     3301 cactaaaaga tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta
     3361 ctgtaatggc tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag
     3421 ggattgaatt ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa
     3481 atcacgcagg aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc
     3541 aaatttgtga aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga
     3601 ataattagcc attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta
     3661 taaaatatta acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca
     3721 tcttaagaaa ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg
     3781 ataaattatt aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta
     3841 ttgtggatga tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt
     3901 atacggtttt caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga
     3961 ttgaccttgc catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa
     4021 aaataaggga caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag
     4081 ataaaattac agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac
     4141 tggagttaat tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa
     4201 aggagcagaa cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg
     4261 agtgttatct gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa
     4321 tcctctgtga aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg
     4381 gcgacgaata tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg
     4441 aaaaaatgaa cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt
     4501 ataaaattga aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat
     4561 atcgttgcaa ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga
     4621 gggaaacttg gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg
     4681 gacgcgatga aattatatca atattccata cggaacaata tagatatctt tatttatgtg
     4741 gcgattgtca ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac
     4801 tttgacgaga taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag
     4861 ctttctgcgg aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa
     4921 aagcgagagc aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg
     4981 gcgcacgata ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag
     5041 gctccagaca tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg
     5101 tatcgactcg aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg
     5161 ataacgctaa caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa
     5221 ttttatcctc agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg
     5281 accgtgtccg gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc
     5341 gctgcataca gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg
     5401 gtgtcaatcg aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt
     5461 gaaaagttct ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga
     5521 ttggcgattg caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat
     5581 gataactata cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg
     5641 aggtcctaag agatgtatat aatt
//
LOCUS       DACCCS010000110         5341 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.118, whole genome shotgun sequence.
ACCESSION   DACCCS010000110 DACCCS010000000
VERSION     DACCCS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.118"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(62..280)
                     /locus_tag="IVK82_002709"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IVK82_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IVK82_002710"
     CDS             complement(546..740)
                     /locus_tag="IVK82_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393715.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            2092..3132
                     /locus_tag="IVK82_002711"
     CDS             2092..3132
                     /locus_tag="IVK82_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP6393716.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3956..4288
                     /locus_tag="IVK82_002712"
     CDS             3956..4288
                     /locus_tag="IVK82_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP6393717.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="IVK82_002713"
     CDS             4907..5248
                     /locus_tag="IVK82_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP6393718.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1770 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtggta tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gaaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttc tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcacaatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattga
     4861 tataaaacat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa gaggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgatgat taataaaaag gtagtattac cttaggcatt gagtaaggaa
     5341 t
//
LOCUS       DACCCS010000111         5322 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.119, whole genome shotgun sequence.
ACCESSION   DACCCS010000111 DACCCS010000000
VERSION     DACCCS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.119"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            10..210
                     /locus_tag="IVK82_002714"
     CDS             10..210
                     /locus_tag="IVK82_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP6393719.1"
                     /translation="MALGRMSELGYSMMFGEVEKNFFMKHIKGRGYVDTGGSVISEFY
                     TPLVPKGYDFLDSISKLEKMND"
     gene            288..962
                     /locus_tag="IVK82_002715"
     CDS             288..962
                     /locus_tag="IVK82_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6393720.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            1195..1629
                     /gene="dcm"
                     /locus_tag="IVK82_002716"
     CDS             1195..1629
                     /gene="dcm"
                     /locus_tag="IVK82_002716"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP6393721.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            1630..2337
                     /locus_tag="IVK82_002717"
     CDS             1630..2337
                     /locus_tag="IVK82_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP6393722.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            2327..2617
                     /locus_tag="IVK82_002718"
     CDS             2327..2617
                     /locus_tag="IVK82_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393723.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            2876..4060
                     /locus_tag="IVK82_002719"
     CDS             2876..4060
                     /locus_tag="IVK82_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6393724.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            4075..4194
                     /locus_tag="IVK82_002720"
     CDS             4075..4194
                     /locus_tag="IVK82_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP6393725.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            4940..>5322
                     /locus_tag="IVK82_002721"
     CDS             4940..>5322
                     /locus_tag="IVK82_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6393726.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMW"
BASE COUNT         1780 a          858 c         1229 g         1455 t
ORIGIN      
        1 aactttcgcg tggctttggg tcgtatgagt gaactcggat attccatgat gtttggggag
       61 gtcgaaaaaa acttctttat gaagcacatc aaaggtcgag gttatgtgga tacaggtggc
      121 agtgtgatta gtgagtttta tacgccactt gtaccaaaag ggtatgattt cttagattca
      181 attagtaaat tagaaaagat gaatgattga gtgatcttca atactgactt tatacttcaa
      241 gtaacgtata atacttttat atagtaggaa gaaaggggat aattaatatg gaaagtaatg
      301 attcttattt agagtatgca aaagcaaata aggataagtt tatttcttat attatcaaag
      361 ggaaatcccc cgaagttgga cgagacgcag tttttatggc agggagtcct ggggctggaa
      421 aatcagaggt tgccattgga ttagctgaga attatgataa ccatgtgatt attgatgcgg
      481 attactttcg cacccagttt cctgattata atggaaaaaa ctctagtctc tttcaaaaag
      541 catcttcttg gttggtagag caggcattta gatatgtctt agatcatggc tattctttta
      601 ttctggatgc aacttttgca atattaagtg ctgaaaaaaa tatctcgcgg gcgttgaaaa
      661 aacattttcg agtgacgatt ttctatgttt atcaagaccc taaaatcgct tggaaattta
      721 cacaagaacg tgaaattgca gagggacgtc atgtgccgaa aaaggcattt attaacgctt
      781 ttttcaaatc aagagagaac attgcaaaag taaagagtaa acatccagat gtattgcttc
      841 atatcataat aaaagattat acgaataata tttccgaagt tcattatgct acggataatc
      901 tagaattatt tttaccacta gggtatagta ggaaagattt ggaggttcaa ttacatgact
      961 gagaaagaac aagtaacgaa aattgtaaag aaatacaata agagcattgc ggatttatcg
     1021 gaaaatgcta ctgcaaaaga gttcaaaacg gtgattaaat atgtagccga tcaggccaat
     1081 gaaaaacaac ggaaattagt tggattaaat aaaaaatagt aggacaaatt aataatgtat
     1141 gagccaagag caaaatgttc ttggcttttt tctttgcaat gaatgaggta atgtatgaga
     1201 tttttagatt tattcgccgg tattggtggc tttcgtttag gtatggaaca ggcgagccat
     1261 cattgtatcg gtttttgtga aattgataaa tttgctagac gtagttataa agcaattcat
     1321 gatacaagta aggaggtgga aatgcatgac atcacaagtg tatcagatga gtttattcaa
     1381 tccctcggac cagtggacat cctttgcggc ggatttccgt gccaagcttt ttcaattgcg
     1441 ggaaagcggc aaagattttc cgatactcga ggtactttat tctttgaaat cgctcggttc
     1501 accactcttc tccgacctaa gcttttattc cttgagaacg tccgaggatt gctcaatcac
     1561 gaaggagggg ctacgttcga gacgatcctc cgaacgctgg atggattggg gtatgacgtg
     1621 gagtggtagg tgcttaactc aaaggcctac attccccaaa gccgggagcg gatattcctt
     1681 atcggacatt ctcgagacgc atgtaccgaa caagtatttc ctatcattgg accgtcttca
     1741 acatctgatc aaaacatcag aaatttggta aatatcaatc cttccaatcg aggaatgggt
     1801 ggacaagtct atggttcaga tggcgtagcg ccaaccttaa caggtgatga aggaatcaaa
     1861 atcgcactgc cagcaaggga tggaatctct gtagcaggac tattaccggg aaagtttgaa
     1921 caaggtaatc gagtttatga agttactgga accgccccca ccttgtcaac gaaacaaggt
     1981 ggtacaaaaa tcatgattcg aaaaaatgaa acagaatatc aagaggtgaa gccagaagac
     2041 agtgtgaatc tggcttttcc taattccaca agtcgccgag gtcgtctggg aaaacagagt
     2101 gttcatacct tactaacagg agaccaacaa gcgatcgtca cggatcagta tcaaattcgg
     2161 aagctaacac ctagagagtg ttggcgactt cagggctttc ccgattgggc ttttgatcga
     2221 gattctcaag tcaattcaga tagtcagttg tataagcaag ccggaaactc tgtaacagtt
     2281 ccggtcattt ttgatattgc cagaagatta aaagagttgg atgtacatgg tttatgatga
     2341 gttgattgga gaaatttatt gggtgattgg aaagattcag tcagacccag aattagaaga
     2401 acaattgcga cggttaaaat ttgatattcg caagaatggt gtcaaagtcg ctggcgatcc
     2461 atatttaatg aatgaggaga cggatgctcg aattgaaata aatcaagtaa ttgcagaatt
     2521 tgagcggatt gcggaccaag caaaagaacc agatattcgt caatacttgt tagaaatgaa
     2581 agcagagttg gaagtaaatg gtattacaga tgagtaacct aaaccaacac aactgactgt
     2641 taggcgctga ccgcggagga ttcgcgaagc agaaatcctc cgtggtgctg tgcctgttca
     2701 tggacggcgt tagccgacca tgatgtctca cccccgtatc taacaggggg gtacaaaaac
     2761 acaaaaaata agtaagcaac cattaagaat gcttgttgta tcaaagatag taagtaattt
     2821 gtataggtgt taactcattt gaaaaagtta acaccttgaa tttggaggtg agaacttggc
     2881 acaacggaat ttagattatc ggctattaaa agaccgacgc aacgaatacg gcgtttcaca
     2941 aaataaattg gctacggctt gtggacttag tcggccgtac ttaaatcaga tagaaaatgg
     3001 tggcgtaacc gcatctataa aaaccatgag gaaaattttt gaccaactgg aaagctttaa
     3061 tcccgatttg cctttgacgt tactgtttga ctatgtaagg attcgctttc ccacaacgga
     3121 tgcacgaaaa attattcaag aaattctcca tttgaaattt gagtatatgc tccatgaaga
     3181 ttatgccttt tactcctatc aagaacagta tgtcatgggt gatattgtgg tgatgttgtc
     3241 tcatgaagaa gataaaggtg tgcttttaga attaaagggg cgaggttgcc gacaatttga
     3301 aactttttta ctcgctcaaa agcgtagctg gtacgacttt ttcgaggatt gtctaaaggc
     3361 cggtggcgtg atgaaacgct tggacttggc aatcaatgat cgagtggggc tattggatat
     3421 acctgagcta acgaaaaaat gtcagaagga agaatgtatc tccttgtttc gtacctttaa
     3481 aagttatcgt tccggggaac ttttaaaggc tgatgaaaag gacggcatgg gcaatacctt
     3541 atatatcggc agtctcaaga gtgaggttta tttctgtttg tatgaaaaag actatgaaca
     3601 atatatcaag ttagggattc cattagacca aactgagacg aaaaatcgtt ttgagattcg
     3661 actgaaaaat gaccgtgctt ttcatgcgat tcaagatttg ttaaaaggtc gtagcattga
     3721 gagcaccacg ttttccatta tcaatcgtta cttgcgattt gcggataaag tagaaggtaa
     3781 aaggcgaacc aactggccct tgaatgaaca atgggggcgt tttattggac ggaatcggaa
     3841 agagattcaa ttaacttccg agccaaaacc gtataccatt gaacgaacct tgaattggct
     3901 aggccgacaa gtcgccccta cgtggaaaat ggctaaggag ttggatcgat tgaatcggac
     3961 aacctatatc caggatatgg tacagaatgc acgcttatca gaccgacata aaaagatttt
     4021 ggagcaacaa agtatggcaa ttgaaaattt gattatatga aaagaggaat cgtcatggga
     4081 attgtaaaag atattttttt ggtatttggt ggcagtatgc ttggtgttac tgtgatgtgc
     4141 ttgatgaatg caagtgcagc agcggatcga gcaatggaaa agaaaggaaa gtgacaagat
     4201 gaatttcgga caaaatttat gtgcgactcg ttccttagtg aaaagctaag gcactaataa
     4261 aagaacgaaa aagtttggtt attaggagaa ctgataatct gacaggtgga atgatgaagt
     4321 aacgctctga aacgcctgcc ctgagcctcc gacaagcatt gtatgaggaa actcagaatg
     4381 tttgaagctc ggtaaagacg gctgaaagat accgtaatag ttgatgaaca tatatcttct
     4441 atcgaaaact atccagaggt gggtagcgta tccaacaggt cgggggtcta taaaatatct
     4501 atggttagaa tgttctataa ggaactgacg aaacagcgaa tgtacgggtc taaatttgaa
     4561 ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg tggcaaagta agaatatagg
     4621 ttatgaaatg ccatataacg ttacaggcgg tatcaagcca gcaggcttat agctagtgac
     4681 ctaaggatat atatgtatag ataagattat cggaacgagg aaaggtattg ggttctcatc
     4741 aagagaataa actgacgaag aacaataagc agttgaacta atgctgaaaa gcagagggcg
     4801 aacctaagaa attcttgtaa tgagaatgga ggaatgccct caagtcgtgt atatattaga
     4861 aaagtattat gttgtttcaa taaggattac gagtaagata aaaaagctca ctcaatttca
     4921 agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa acattgtgtg tagaagattt
     4981 aagacacgct gaatattacg aaatgcaaaa cacctttgat gatttatatg ccaaaagtaa
     5041 gaatggagat attttcactc atcttatgga tattatccta tcgagagaaa atatattact
     5101 ggcatatcga aatatcaaag caaatgcagg cagtaaaaca gcaggaacag acggtacaat
     5161 aattaaagat attggcaaat tgccagcaga aacagttgtc aaaaaagtaa gatacattgt
     5221 tgcaggcagt ccacatgggt atcgacctaa gccagtgagg cgaaaagaaa taccaaaacc
     5281 aaatggtaaa accagacctc taggtattcc ttgtatgtgg ga
//
LOCUS       DACCCS010000112         5290 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.120, whole genome shotgun sequence.
ACCESSION   DACCCS010000112 DACCCS010000000
VERSION     DACCCS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.120"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            8..1885
                     /gene="tdc"
                     /locus_tag="IVK82_002722"
     CDS             8..1885
                     /gene="tdc"
                     /locus_tag="IVK82_002722"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP6393727.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            2128..3549
                     /locus_tag="IVK82_002723"
     CDS             2128..3549
                     /locus_tag="IVK82_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6393728.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            3658..5025
                     /gene="nhaC"
                     /locus_tag="IVK82_002724"
     CDS             3658..5025
                     /gene="nhaC"
                     /locus_tag="IVK82_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP6393729.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT         1578 a          973 c         1128 g         1611 t
ORIGIN      
        1 tttcactatg agtgaatcat tgtcgaaaga tctaaaccta aatgccctat tcattgggga
       61 caaagctgag aacggccaaa tctataaagc tttgttgaat gaattggtgg atgaacattt
      121 aggctggcgt caaaactaca tgcctcaaga catgcctatt attacgccag aagaaaaaag
      181 cagtgctagt tttgaacaca ctgtgaataa aacaaaggat gttctatcag aaatttcagc
      241 gcggatgcgt acccattccg ttccttggca taatgccgga cgttactggg gtcatatgaa
      301 ctctgaaaca ttgatgccat ctttactagc gtacaatttt gctatgctat ggaatggtaa
      361 taacgttgcc tatgaatctt ctcctgcaac aagccaaatg gaagaagaag taggaatgga
      421 atttgcaaaa ttaatgagct ataaagatgg ctggggccac atcgttgctg atggttcttt
      481 agcaaactta gaaggacttt ggtatgcccg caacatcaaa tcattgccgc ttgcaatgaa
      541 agaagtgaca cctgaactag ttgctggaaa aagcgattgg gaattaatga acttgtcaac
      601 agaagaaatc atgaatctgt tagacagcgt cccagaaaaa atcgacgaaa tcaaagctca
      661 ctcagcacgt agcggaaaac atttagaaaa attaggaaaa tggttggttc cacaaactaa
      721 acactattca tggctaaaag ctgccgacat catcggtatc ggtttagacc aagtgattcc
      781 agtgccagtt gaccacaact atcgcatgga catcaacgaa cttgaaaaaa tcgttcgtgg
      841 tctagcagct gaaaaaacac caatcctagg tgtagtcggt gttgtaggat caacagaaga
      901 aggagcaatc gacggtatcg ataaaatcgt tgcattgcgt cgcgtattag aaaaagacgg
      961 tatttacttc tacttacacg tagatgctgc ttatggcgga tacggccgtg cgattttctt
     1021 agacgaagac aacaacttca ttccatttga agatttaaaa gatgttcatt acaaatacaa
     1081 tgtctttaca gaaaacaaag attatatctt agaagaagta cacagtgcat ataaagcaat
     1141 cgaagaagca gaatctgtaa cgattgaccc acataaaatg ggctatgttc catattctgc
     1201 cggtggtatc gtcatcaaag atatccgcat gagagacgtt atctcttact ttgcgacata
     1261 tgtatttgaa aaaggcgcag atatcccagc attacttggg gcttatatct tggaaggttc
     1321 aaaagcaggg gcaacagcag ccagcgtttg ggcagcacac catgtcttac ctttgaatgt
     1381 cacaggatat ggtaaattaa tgggtgcatc aatcgaaggt gcacatcgtt tctacaactt
     1441 cttgaatgac ttgtcattta aagtaggaga caaagaaatc gaagttcatc cattaactta
     1501 tccagatttc aatatggtag actatgtatt caaagaaaaa ggcaacgatg acttagttgc
     1561 aatgaataaa ttgaaccatg acgtatatga ttattcttca tacgtgaaag gaagcattta
     1621 cggtaatgag ttcttaactt cacatactga ctttgctatc ccagactatg gcaacagccc
     1681 attacaattt gtgaaccaac taggattctc agatgaagaa tggaaccgtg ccggaaaagt
     1741 aactgtattg cgtgccagcg taatgacacc atacatgaac aaagaagaac actttgaaga
     1801 atatgctgaa aaaatcaaag cagctcttca agaaaaattg gaaaaaatct acgcagacca
     1861 attattggca agcgaagcaa aataagcaag gtaatcatgt tagaaaacag cttgaatact
     1921 aactgtttct gacgcatgtt tggatctgaa gagcaagggg agacctaagc tcttcgatcc
     1981 ctttgttttg aggttgtctt tgaagaaacc cttcaagcaa taaactcaaa gaatcccctc
     2041 aaatattcgc tgaattgttt cgtttattgc tgaaactact tcaaagacac cgttatttta
     2101 tccaaaaaat caaggagagt gtcaacggtg agtaaatctg aagtaagtaa aatgggtctt
     2161 gggaccttta tcggtctgac aatggcactg tgtgcaacgg ttcggagtat tcctacgtta
     2221 gccgcagtcg gttggacatt gattttttat tcaatttttg cagttttgtt ttttgccggt
     2281 ccgatatcaa tggtttccgg tgagctttcc acgatgcttc ctcaagaagg cggtcctcag
     2341 ttatgggtaa aaacagcact gggcagcaaa tggggatttg tcgttgcatg gctattatgg
     2401 gtgcaaatgt tcccaggtat ggtaatggtc gcttctactt taggaccatt gttaggaaac
     2461 acatttggta atgtagaact tggcaataat cactggttcg ttttaggttg tattttagtt
     2521 atctactgga tcatcacgat cttgaacttg aaattcgata tggcaaaagt cggcgggaac
     2581 atcggtgtat ggctaggggt atatatccca gtagtcatca tgttcgtttt aggtgttttg
     2641 gcagccttca aagtcggact agtctcaaac ggctatctag gtgacttttc atggtcaaaa
     2701 gctttccctg acttggaaca tattgattca ttgaaatact tagctggtat cacatttatt
     2761 tttgttggga tcgaaatgag ttctgtttac atgcctcgtt tgaaagacgc aacaaaaaat
     2821 tatacaaaag gcgtatttat cgctttgatc ggattagttt tattgaatgt catcaatgcg
     2881 atgcttgtag caaatgtcgt accagatgga aaaatggaat tagcaaatat cacgcagcct
     2941 atcttgattg actgtcaaat cctaggctta ccagaagtga tcggaaatat cttcagcttc
     3001 atggtattta ttggtgtgct tttacaatta tcagcttggg ttactggacc atcaaaaaca
     3061 atcatccaag tagctcgtga aggattcctt ccaccaaaat tcggattcca taaagaaaac
     3121 aaatacggcg tatctagaaa tgtcgttcta acacaatcaa tcgttatttc attatttgct
     3181 ttgctttatg gtgtaatgga tgacgtaagt gctgtattct tgacacttac aaatgctaca
     3241 actgttattt attgtatcgt gtacattttg attgctgttt cattgcttaa aatgcgtaag
     3301 aaacatccag aattcgaacg tccttaccga attggtaaaa acggaaacgg attagcttgg
     3361 gtagtcagct gcatgctgat cttctctatc atcgttgtcg tatttgctac attaggtaca
     3421 gcgacattga gtgatgcatt attagtagca gctatcacag tggtcatgtt cgttattcca
     3481 ttgatcatca atcacttcaa gaaagattct tgggggattg aggtagaaaa aagtttagaa
     3541 gaaaaataaa aaaataagtc tttcagccga cgttttgtcg ctgatttagt aggaggtcct
     3601 gccaagtgaa tagttttttc atggcagggc cttttcattt gaaataggag atcgacgatg
     3661 aataaaaata tttctttaaa agaaagtatc gctgtattga tcgtgttgtt ggcactccta
     3721 ggagtactga tcatcggatt ccagctctcg ccacatatcc cgatccttgc agctttcatg
     3781 tgtttgttgt tttatggcag atttaaaaag ttcacatggg atgagatcca tgacgggatc
     3841 atcaaaggaa tcactccagg gatcattcca atcctgattt ttcttttgat tggtgtctta
     3901 gttgcttcat ggattttatc tggaaccatc cctacgatta tggtttatgg atttaaactt
     3961 gtttctgtgc gtttcttttt accgacagca tttcttgttt gtgcaattgt cggagtgact
     4021 gtcggcagtt catttacaac gatttcgacg attggaattg cttttttggg gatcggtcat
     4081 ttacttggat tcaatgatgc aatgactaca ggcgcagtcg tttccggtgc ctttttaggg
     4141 aataactgct cgcctttatc agatacgacc aacttggcag cgggtattgg aggagttaat
     4201 ctattcgaac atatcgctgg tatgcgttat acggatttcc cagcattcgt cctttcgttg
     4261 attttttata cgatattagg acataacagt cattcagccg atctagctag tatcaatgaa
     4321 atgatccaaa acatgaaagc gagcttttgg atctctcctt ggacattgat tccactagtt
     4381 attttatttg ggggggcaat caaaaaaatt ccggctatcc ctacattact agcgggatcg
     4441 acagctgctc tcgttctttc ttttatccat gagaaaagtt tgacggtcgg aaaagcagca
     4501 gacatcttga tgaatggtta tgtcgcaaat acacctgatc caaagctaga tgaactgctt
     4561 tctcgaggcg ggatcatgag tatgttaggt tctgctagtt tgattttact tgcattggca
     4621 ttaggtgggt tactgatcaa atatctgatc gttgatacca tcgttcaaga attaaaagaa
     4681 aagatggacc gaccttctcg tttagtcggg tttactgcct tgagctgtat cggaatcaat
     4741 ctgatcgttg gagaacagta tctgtccatc attcttccag gagaaacatt caagcgttct
     4801 tttgatgacg ttggcttatc aaaaacctat ttgacacgaa cactagcgga ttcaggtgcg
     4861 accgtcaatt ctctcgtacc gtggggagtc agcggcacat tcattatggg aacgatgaaa
     4921 gttagcgcgt tgcattactt gccattcgcc tttttctcga ttcttgcacc tgtctttaca
     4981 atattaggcg gttttttatt gaatcgtcga cgtgtaaaaa attgaaggaa ggaaaaaaga
     5041 ggctgggaca gaagcgttta actccaagaa ataagaagaa actcaagaaa attgcttttt
     5101 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ccgtttatac
     5161 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt ttttacagat
     5221 aaacaatgtg ttagaagtag tatcttcttt tacataaaaa gctgaccata ggataaatgg
     5281 tcagcgtatt
//
LOCUS       DACCCS010000113         5289 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.121, whole genome shotgun sequence.
ACCESSION   DACCCS010000113 DACCCS010000000
VERSION     DACCCS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.121"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..410)
                     /locus_tag="IVK82_002725"
     CDS             complement(<1..410)
                     /locus_tag="IVK82_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6393730.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHFVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEK"
     gene            complement(492..842)
                     /locus_tag="IVK82_002726"
     CDS             complement(492..842)
                     /locus_tag="IVK82_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393731.1"
                     /translation="MINNYLHYKAINWNVIEDELDNVVWERATSLFWLDTRVPIENDR
                     SKWANLQLQEQEQLNRLLILLTNIATYQSNELGEIIRDSARSQQEIAIINNFQFTEMV
                     HTKAYNRILSIRQF"
     gene            complement(891..1334)
                     /gene="nrdI"
                     /locus_tag="IVK82_002727"
     CDS             complement(891..1334)
                     /gene="nrdI"
                     /locus_tag="IVK82_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP6393732.1"
                     /translation="MQIIKVYYISLSGNTTSFLADLDTYCQKQYQKKVDYVNVKDLVK
                     LGKPLTFDINESYFVFLPTYLEGGNGIETGDVEILTTPLRQVITYNKNRDYCLGIIGS
                     GNRNFNKQFCLTAYQYADDFGFTVLDEFELKGTTEDIERIAETMQ"
     gene            1481..2146
                     /locus_tag="IVK82_002728"
     CDS             1481..2146
                     /locus_tag="IVK82_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6393733.1"
                     /translation="MKNSGCLCTVPLFASLSLDEKNEIKCLLHHQTFKKGEIIFSPSS
                     EEQLLIISLGAMKIYRISRTGKEQLIRIVKEGDYDGENYLFGLPNDCLYGEAVEETKT
                     CTLYKSDFNKVMARYPQLSYRLLQLNACKTVAVEKQLQLLAFDRIEDRLALYLNQLSI
                     AAKMGEPITLPISMRELANYLATSPETISRKLRDFQERQLITRKGKQITLLEEFWFIF
                     NYI"
     gene            2136..2591
                     /locus_tag="IVK82_002729"
     CDS             2136..2591
                     /locus_tag="IVK82_002729"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAP6393734.1"
                     /translation="MIYKEEDIKIGIVIARFNDFLTKSLLSGCEDGLTRSGVLTENID
                     VAWVPGAYEIPLTAATMAETGKYDAIITLGAVIRGATSHFDVVVNQASSGVSQVGLTH
                     KLPVIFGILTVENIEQAIERSGTKAGNLGFTYATNTIEMINLFKEIKQQ"
     gene            2741..4120
                     /locus_tag="IVK82_002730"
     CDS             2741..4120
                     /locus_tag="IVK82_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAP6393735.1"
                     /translation="MTIQFKQTLAPTKDNSGKPIILSDATFAQRNEKVLQLMREYDFS
                     SLMIYADKEHGSNFEYLTGFIPRFEEAIQVINQDGTSTLILGNENYNKTSMARTQSEG
                     ILCPFFSLPNQPMDFTKTFADYLKDVKVDASKRIGLVDWKLLSNDMDNFHNHHSIPAF
                     IVDGIHEVFGSDKVVNATQLYMHPKEGARVTNNAEEIARYEYGASLASDAVLEAMNQL
                     TVGQSELAAGNHLNRDGQYPSVVTIAAFGDRFEGANIYPTDNTLNDGDKVALTVAYKG
                     GLSSRSGYAVKEESQLAEHDPGYLEEVVYPYFQAYKWWLDNLTIGRKGGDFYQAFSDF
                     YPQATYGWNLCPGHLVADEEWLSSPFYQDSDATIQSGYIFQLDFIPSQAGHNGVSAES
                     TIAVADAALREQIKAEQPELWARICNRKDYLKEALGLELSEHLLPLASTFAYYRPFLL
                     DKKTAIYFG"
     gene            4257..5099
                     /locus_tag="IVK82_002731"
     CDS             4257..5099
                     /locus_tag="IVK82_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP6393736.1"
                     /translation="MPLDEQIELLFTIDEGYLNPLKVALTSIRQNNPGQAFRIWLIHE
                     SIAMHTIRELQKLTDYLQFGFEAIKIDGSRWNSAKTEDRYPKEMYFRLLAGEILPKEM
                     KRVIYLDPDILVVNPLLDLWQTDLEGYMLAAATHVGLTDVSTRVNQMRLDVDHAYYNS
                     GVMVIDLDKAREIIKWSDIAQMIEKYNLLLILPDQDILNRLYGKYTKEIPEEIWNYDT
                     RKYMRYFTKSLAQHDIHWVMAHTAILHFCGGPKPWDDKHDNRFTSLYLTYQNQLKRIE
                     DLMK"
BASE COUNT         1678 a          931 c         1003 g         1677 t
ORIGIN      
        1 cttttttctt tatgtccgtt tggttttctt ttccctttca ttggtaaatc gtgagggtca
       61 aaggttttat ctttaaacat ccgatatagt gtacgtaccg aacaagcaat aggaaactct
      121 gctcgaccaa taaggacatc tggagtccac ccttgagcca cctttttttg aatgtattct
      181 tgttgcccat ctggtaacac aatcggacgt cttccgcagt tacgtttgtt tttcttatat
      241 tgttcatagt agcttattgc gcttccacca gatttaagaa aacgatagac attataaatg
      301 gtttgtcgag aacgttgtac tagatgggct acttttgaaa caaagtgtga ttgattgaaa
      361 taagcttcta tcatcacgag ttcagttggt gtaagatggg tataggtcat ttgtactcac
      421 tccttataat tctttcgacg gaactatttt gagtgtagca caaatgactt ttttagttgt
      481 ctagcttaat tttagaattg gcgtatgctt aaaatgcggt tataggcttt tgtatgaacc
      541 atttcagtaa attgaaaatt atttatgatt gcaatctctt gttgggaacg tgcactatct
      601 cgaataattt ctcctaattc attggattga taggtagcga tattagtcaa aagaatcaac
      661 aagcgattga gctgctcttg ttcttgtaat tgtaaatttg cccattttga tcgatcattt
      721 tcgatgggga cacgtgtatc caaccaaaaa agtgaggttg ctctttccca tacaacgttg
      781 tctaattcat cctcaatgac attccagtta attgctttat agtgtaaata attatttatc
      841 atagtacctc ctaaaatatt gtttgaaagg ggatgatatg gtgtcatttt ttattgcatt
      901 gtttctgcaa ttctctctat atcttctgtc gtacctttta gttcaaattc atccaacacg
      961 gtaaaaccaa aatcatcggc gtattgatag gctgttaggc aaaattgttt attgaagttt
     1021 ctgttgccac taccaataat tcctaaacaa tagtctcggt ttttattgta agtaatcact
     1081 tggcgcaatg gtgttgtcaa aatttcgaca tctcctgttt caataccatt tccaccttca
     1141 agataagtag gcagaaatac aaaataggat tcattgatgt caaaagtgag tggttttcct
     1201 aatttgacta aatcttttac attcacataa tctaccttct tttgatattg tttttgacaa
     1261 tacgtatcta aatctgcgag aaaactcgtg gtatttccac ttaaactaat ataatagact
     1321 ttaattattt gcatgtatca cacctctttt taataaataa ccacatatat agtgcttgtt
     1381 ttaattagaa acgctatata tagtgtttat tgtacatgac atatcgttat agaaaattga
     1441 cgatggtcaa ttattttgat tttagaggag agaaaattac atgaaaaatt caggttgttt
     1501 atgcaccgtt ccactcttcg cctctctttc attagatgaa aaaaatgaga taaagtgttt
     1561 gcttcaccat cagacgttta aaaaaggaga aattattttt tcaccaagta gtgaagaaca
     1621 actgttgatt atatctttag gggctatgaa aatatatcga attagtagaa ctgggaagga
     1681 gcaattaatt cgtatcgtaa aagaagggga ttacgacggt gagaactatc tctttggtct
     1741 acccaatgac tgcctatatg gtgaagctgt tgaagaaacc aaaacttgca ctctctataa
     1801 atccgacttt aataaagtga tggcacgcta tccacagctc agttatcgat tgttacaatt
     1861 aaatgcttgc aaaacagtag ctgtggaaaa acagttgcag cttctagcgt ttgatcgtat
     1921 agaggaccgg ttagccttat atcttaacca actatctata gcagcaaaaa tgggggagcc
     1981 tattacttta cctatctcta tgagagagtt ggcaaactat ctagctacca gtcctgaaac
     2041 tatttctcgt aaattaagag attttcaaga aagacaattg attactagaa aaggaaagca
     2101 gattacacta ttagaagaat tttggtttat atttaattat atataaagaa gaagatatta
     2161 aaattgggat tgtcattgca cgattcaatg attttttaac taaaagctta ctaagtggtt
     2221 gtgaagatgg ccttacgcgt tcaggcgttt tgacagaaaa tatcgatgtt gcttgggtgc
     2281 cgggggccta tgaaatccca cttacagccg caacgatggc tgaaacaggt aaatatgatg
     2341 ccattattac cctaggtgcg gtaatcagag gagctacaag tcactttgat gtggtggtta
     2401 accaagcgtc aagtggggta tctcaagtag gcttaaccca taaattaccg gtcatctttg
     2461 gtatcttaac cgttgaaaac atcgaacaag ccattgaacg ctctggtact aaagctggta
     2521 accttgggtt tacttatgca acgaatacta ttgaaatgat taatttattc aaagaaatta
     2581 aacaacaata agtcatcatt ctatatgaaa atatctgtaa cagcataggg ctagctttaa
     2641 ttttcaatca tggtaagatg gaagatagga aaataaagcg cattcaatta attatcaagc
     2701 ctttctgatg atttgcgcaa aaagcttaag gagacttcat atgacgattc aatttaaaca
     2761 aacattagcg ccaaccaagg ataattctgg aaaaccaatc attttaagtg acgctacctt
     2821 cgctcaacgt aatgagaaag tccttcagtt aatgcgtgaa tatgacttct catcattgat
     2881 gatttatgca gataaagaac acggtagtaa ctttgaatat ttaactggtt ttattccacg
     2941 gtttgaagaa gctatccaag taatcaacca agacggaacg tcgacactga ttttaggtaa
     3001 tgagaattac aataaaacca gtatggcacg tacacaaagt gaaggtattc tttgcccatt
     3061 cttctcttta ccaaaccaac ctatggattt cactaaaact tttgctgact atcttaaaga
     3121 cgttaaagta gatgcttcaa aacgaattgg tttagtagac tggaagcttt tatctaatga
     3181 tatggacaac ttccacaacc atcattcaat accagcattt attgtagatg gtatacatga
     3241 agttttcggt tctgacaagg tagtgaatgc aacccaacta tacatgcatc caaaagaagg
     3301 tgctcgtgtg acaaacaacg cagaggaaat cgctcgttat gaatatggtg cttcccttgc
     3361 ttctgatgct gttcttgaag caatgaacca gctaaccgtt ggccaaagtg agttagcagc
     3421 aggtaatcac ttaaatcgcg atggtcaata tccatctgtt gtgaccattg cagcctttgg
     3481 tgatcgcttt gagggtgcta atatttatcc aactgataat acgctaaatg atggcgataa
     3541 agttgcatta actgtagcct ataaaggagg cttgtctagc cgctctggct atgcagtaaa
     3601 agaggaaagt cagctcgctg aacatgatcc aggatactta gaagaagtgg tttacccata
     3661 tttccaagcc tataaatggt ggctagataa tttaaccatt gggagaaagg gtggcgattt
     3721 ctaccaagca ttcagtgact tctatccgca agctacttat ggctggaatt tgtgcccagg
     3781 gcatttagta gctgacgaag aatggttaag ctcaccattt tatcaagact ctgatgcaac
     3841 aattcaaagt ggctacatct tccaactaga ctttattcct tcacaagcag gccacaatgg
     3901 tgtttcagct gaatcaacga ttgcggttgc ggacgcggca ctacgtgaac agattaaggc
     3961 tgaacaacct gaattatggg cacgtatctg taaccgaaag gattatctaa aagaagcatt
     4021 aggtttagaa ttatcagaac atttactacc cttagcgagt acctttgcct actatagacc
     4081 tttcttatta gacaaaaaaa ctgctatcta ctttggataa aaaaatgccc ctcaagattg
     4141 tttgccaaac ttgagggttt tttgtctatt tttaagtgaa taatgacgga ctatccaaac
     4201 ggaatcgcca taaagatgtt aatatatgta taatattata aagaaattgg aggacaatgc
     4261 ccttggacga gcagattgaa ttattattta ccatagatga aggctatttg aatcccttaa
     4321 aagtggcttt aacatcgatt cgtcagaata atccaggcca agctttccgc atttggttga
     4381 ttcacgaatc cattgcaatg catacgattc gcgaattgca gaagctaacg gattatctac
     4441 agtttggttt tgaagcgatt aaaattgatg gtagtcgatg gaatagcgct aaaactgaag
     4501 atagataccc aaaagagatg tatttccgcc tgcttgcggg tgaaatatta cctaaagaaa
     4561 tgaaacgcgt gatctattta gacccagata ttttagtggt taacccttta ttagatttgt
     4621 ggcaaacaga tttagaaggt tatatgctag cagctgctac acatgttgga ttgacggacg
     4681 tttctacacg agtaaaccaa atgcgacttg acgttgacca tgcttattac aattctggtg
     4741 tgatggtgat tgatttagat aaagctagag aaattattaa atggtcagat attgcacaaa
     4801 tgattgaaaa atataacttg ctattaatcc tacccgacca ggatatcctc aaccgattat
     4861 atggtaaata cactaaggaa attccagaag aaatttggaa ttatgatacg cgtaaatata
     4921 tgcggtattt caccaaaagt ctcgctcaac atgatattca ctgggtgatg gcgcatactg
     4981 ctattctcca tttctgtggt ggcccaaaac catgggacga taaacatgat aatcgtttta
     5041 ccagtcttta tcttacctat caaaatcaat taaagcgtat tgaagatttg atgaagtaaa
     5101 gtgaaacaag tgtttttcat tacctacggt ggtttaatgc ttatttatta tcgttaaatc
     5161 atgcttatac actttacaaa atttgatttt cgaaatggcc gtccaacgaa gtctgcgatc
     5221 ttaaccacga gttccattga catggccaaa aagtattatc gagcgatcaa agaaatgacg
     5281 aaagaacct
//
LOCUS       DACCCS010000114         4807 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.123, whole genome shotgun sequence.
ACCESSION   DACCCS010000114 DACCCS010000000
VERSION     DACCCS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.123"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IVK82_002732"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IVK82_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP6393737.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="IVK82_002733"
     CDS             complement(3250..3768)
                     /locus_tag="IVK82_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP6393738.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1579 a          786 c          730 g         1712 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccactta
//
LOCUS       DACCCS010000115         4784 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.125, whole genome shotgun sequence.
ACCESSION   DACCCS010000115 DACCCS010000000
VERSION     DACCCS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.125"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            544..1107
                     /locus_tag="IVK82_002734"
     CDS             544..1107
                     /locus_tag="IVK82_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6393739.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="IVK82_002735"
     CDS             complement(1386..1673)
                     /locus_tag="IVK82_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6393740.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="IVK82_002736"
     CDS             complement(1663..1992)
                     /locus_tag="IVK82_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6393741.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="IVK82_002737"
     CDS             2297..2626
                     /locus_tag="IVK82_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6393742.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IVK82_002738"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IVK82_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAP6393743.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /locus_tag="IVK82_002739"
     CDS             complement(3551..4462)
                     /locus_tag="IVK82_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAP6393744.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1564 a          824 c          722 g         1674 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ctgccttaca tctt
//
LOCUS       DACCCS010000116         4773 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.126, whole genome shotgun sequence.
ACCESSION   DACCCS010000116 DACCCS010000000
VERSION     DACCCS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.126"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..196)
                     /locus_tag="IVK82_002740"
     rRNA            complement(<1..196)
                     /locus_tag="IVK82_002740"
                     /product="16S ribosomal RNA"
     gene            116..259
                     /locus_tag="IVK82_002741"
     CDS             116..259
                     /locus_tag="IVK82_002741"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393745.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            complement(882..2387)
                     /locus_tag="IVK82_002742"
     CDS             complement(882..2387)
                     /locus_tag="IVK82_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6393746.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2384..3070)
                     /locus_tag="IVK82_002743"
     CDS             complement(2384..3070)
                     /locus_tag="IVK82_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6393747.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(3263..4684)
                     /gene="gndA"
                     /locus_tag="IVK82_002744"
     CDS             complement(3263..4684)
                     /gene="gndA"
                     /locus_tag="IVK82_002744"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP6393748.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1317 a          995 c          800 g         1661 t
ORIGIN      
        1 gattgttata cggtattagc acctgtttcc aagtgttatc cccttctgat gggcaggtta
       61 cccacgtgtt actcacccgt tcgccactct tctttttccg gtggagcaag ctccggtgga
      121 aaaagaagcg ttcgacttgc atgtattagg cacgccgcca gcgttcgtcc tgagccagga
      181 tcaaactctc ataaaaagtt cgaacaatct tgcgattgtt aagctcaatt tgtttgctag
      241 catattgctt gcttgttaaa aatgtttgtt gtcttgaatt gaatcaagac gccctacaca
      301 tttggtttgt cttactttgt tcagttttca aaggtctaat ctttttgttt taacaactct
      361 ttaatcttat cagattcaga agagaatgtc aacaacttct ttgtttattt tttctaaacg
      421 tctttgccac tcattagcag cgacttgatt atcataccat ctttattgtt atttgtcaat
      481 aactttttcg aagttttttt cgataaaatc aagctttatt agctatctca tccaaacgac
      541 ttcgttatta taacatgaca tcaaaagtgc gtcaacacta ttttttataa tttttagtga
      601 caccttaaag gaatcattta gaacaaataa taatatagca atctctttat aaaattgcaa
      661 tagaaaattc cctattttta tttattattt ttaaacacga atattacagc attttattgt
      721 tttttcgttc atctaaaaga tacttacatt taattaacgg atgttattct atttaattgt
      781 tcgctagttt ttttataatg acatccctga atttctcact tgcttgtatt ttgtacctta
      841 ttctttgttg gatatataaa aaaacaagta aagcacatgc tttacttgtc ttctgccttt
      901 gcgataggaa taaagattct aaaaattgtc ccttgcccta ctacactttc tgctaagata
      961 cgtcctttat agttttcaac cagttgttta gcgatagaca accccagacc atttccaccc
     1021 ttcgttctag ctcttgcttt atccactcga taaaaacgat tgaagatctt attaaggtct
     1081 tctttgggta ttccttcgcc aaagtcctga atcgcgatct caaattcatt cagagtggaa
     1141 gaaatagaga tatggacttc ttttcgtgta gttgaatact ttactgcgtt atccaatatg
     1201 ataatgatta attgttcaaa gtgattacga taaatttgca gggtcacttc ttttgtcaaa
     1261 tcatcatcta aagtgatcac atagtctgga tataatatct tgaaattatt ataaacttga
     1321 taggtgactt cttttccttt agttgtttca ttcgcataat gaacatctac ttgttctgct
     1381 cttgataagt ctagcatctc ttgaactaag cttttcatac ggctgatttc ttgcatgctg
     1441 gcgcttaatg attcttctaa tatctcagga tcatctttcc cccaacgatt tagcagacta
     1501 agatgccctt caataattgc aacaggtgtc cttaactcat gagatacgtc ttccacgaat
     1561 tgctcttgtt gttcaatata caaacgcata cggtcaagca tctcattgaa tatttcagct
     1621 aaatctgcca attcatcatt tgtgtcgatt tcaggcatgt ggatatctga ttgcggatct
     1681 ttacgtatag tatccattgt atctctgagt accttgagtg gttttaagaa ataagaagat
     1741 aaaaagaaac ctaaaatact acttaagatc aaagaaacaa cttctaaaac aataagagtc
     1801 aataatagct tatttcgaat atcatagaaa gaagataatt catagaagct ttgcgcatag
     1861 ccgatctttt ctcttgtacc tttagaatat ataggctgga tagataaaaa gccagttttt
     1921 ccttctagtg taacgatcgt tggaacttta cgtgtagtct gaatcaggtt tcgactgttt
     1981 tcatgggttt taaaaatcaa gttctcgtcc aaatcataaa cataaagatc cattgatggc
     2041 tgccctagct cagctataaa ggaatcgatc ttcattaagt ttccttcgac cagtgaagta
     2101 tgatctacga cctcatctcc gtcatttgtc gctttcgtca gattacgata cgtatttact
     2161 aatgtcagtt cttcatccga gttagccaaa cgagaagcta cttctgaaat cgtacgctct
     2221 acattgtttc tttcttttgc cacaatcaga ttgatagatg atttataggt aatgacagcg
     2281 aaaattgtaa acacaacgaa tatgaagaaa gaactcgcaa aggcccactt gattgtcaag
     2341 gagggccctt tcagttctct tttttccgat tttttcggct ttatcacgag cgcatcacat
     2401 aacctgttcc gcgtacagtt tggatatagc tttcctcccc gggaacgtca attttgttgc
     2461 gaagataacg aatatatacg tccactacgt ttgtttcaac ttctgtttcg tatccccaca
     2521 ctttatttag taaaacgtca cgtgctaata ccacgttcac gttttccatt aaagtaagca
     2581 gcaactcgta ttctcttttt gttagttcaa ttacttcatt tccgcgacgt acgacacggt
     2641 tttctttttc gatagtcaaa tcgcgataag taatagtagt ttgtttagcc acatttttat
     2701 cgccttcaat atcgattcga cgcaacaatg cacgaagacg tgctaataat tcttcgatag
     2761 caaatggttt gacgatgtag tcatctgctc cgtggtcaag tccagataca cgatcgatca
     2821 cagaatctct tgcagtcatc ataatgattg gtgtattttt gacttggcgt acacgtctgc
     2881 agacttctaa accgttcaat tctgggagca tcagatccaa caatatggca tcccactcat
     2941 tatttaatgc cgcatctaaa ccagttcggc cgttgtagtg gacttctgcg ttataccctt
     3001 catgcttcaa ctctagctcg acaaatcgtg ccaagttctt ttcatcttca atgattagaa
     3061 tattgctcat ttattatttt atcctttcta ttctactggc tttttaaaaa ctctatcatt
     3121 ataccgataa tatgcttaaa aatctatagt cattttatca tggatagcaa ttttaaaaaa
     3181 gagctgaagt ctactcataa caatcgactt cagctctttc gtcctctttt tattttctca
     3241 tttcctaaca gccgaaagac tattattctt catggtacca tgagtaatgg aagattcctt
     3301 ctttgtcgac acgctcatag gtatgtgcgc caaagtaatc gcgttgggct tggattatat
     3361 ttgcaggcag acgttctgct ctgtatgaat cgaagtaagc gatggctgat gagaaagtcg
     3421 gtacaggtac gccagcttgg acagccaagg caacgacgtc tctgactgat tgctgatatt
     3481 ttttcgtgat ttcaacgaag taatcatcta gcaataaatt ttcgatctca ggatttttgt
     3541 cataggcatc cgtaattttt tgcaagaatc gagctcggat gatacatcca gcgcgccaga
     3601 tttttgcgat ttcaccaaat ggcaagtccc aatcaaattc ttttgaagcg acacgtaatt
     3661 gagcaaatcc ttgggcataa ctcatgattt tactgaaata caacgcttct cggatttttt
     3721 cgaccaattc ttttttgtct cccgtgaact caaaatcgtt cgtacgagac aaaatcttgc
     3781 ttgcttgtac gcgttcttct ttataagcag aaatatagcg ggcaaataca gattcagtga
     3841 tcaatggaag tggcacacct aagtcaagcg cgctttggct tgtccattta cctgttcctt
     3901 tattcccagc tgcatcaagg atcacatcta ctactggttt acctgttcct tcatcgtctt
     3961 tacgtgtcag aatgtcagca gtgatttcta tcaaatagct gtctaattcg ccttgattcc
     4021 attctttaaa gatatcagcc atttcttcta ctgaaaggtt taaaatattc ttcattaagt
     4081 cataagattc agcaatcagt tgcatatctc catactcaat accgttgtgt accattttca
     4141 cataatgtcc ggctccattt gggccaatat atgtgacaca tggtgctccg tcttctgctt
     4201 ttgcagaaat ttgctctaag attggtgcaa ctagcgcata ggcttctttt tgtccaccag
     4261 gcatgattga aggtcctttt aaagctcctt cttcaccacc ggaaacgcct gtaccgataa
     4321 agttgatgcc agagttggct aactcttcat tacgacgcat cgtatcttta aagaatgtat
     4381 ttcctccgtc aatcaaaata tcgcctttat caaggtgagg aagtaactct tggattgttg
     4441 catctgttgc cggtcctgct ttgaccatta gcaagatccg acgtggtttc tcgatagatt
     4501 ctacaaattc ttcgattgta tatgttgctt tgaaattttt atctgggtgt tcttctacta
     4561 cagcggttgt tttagcccct gtacgattga ataaagccac tgaatagccg cggctttcta
     4621 tgttcaaggc tagattttta cccataacgg ccataccgac aacgccgatt tgttgttttg
     4681 acatatgaac tcctcctaca tacgggattt ctccctaatc tcttacatta tagcttaaca
     4741 cggctacatt gcaaactaaa gaacaacata ttt
//
LOCUS       DACCCS010000117         4629 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.127, whole genome shotgun sequence.
ACCESSION   DACCCS010000117 DACCCS010000000
VERSION     DACCCS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.127"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(129..359)
                     /locus_tag="IVK82_002745"
     CDS             complement(129..359)
                     /locus_tag="IVK82_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP6393749.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(589..1407)
                     /locus_tag="IVK82_002746"
     CDS             complement(589..1407)
                     /locus_tag="IVK82_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6393750.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1568..2257
                     /locus_tag="IVK82_002747"
     CDS             1568..2257
                     /locus_tag="IVK82_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP6393751.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2271..3773
                     /locus_tag="IVK82_002748"
     CDS             2271..3773
                     /locus_tag="IVK82_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6393752.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3786..4262
                     /locus_tag="IVK82_002749"
     CDS             3786..4262
                     /locus_tag="IVK82_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6393753.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4359..4469)
                     /locus_tag="IVK82_002750"
                     /pseudo
     CDS             complement(4359..4469)
                     /locus_tag="IVK82_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1549 a          664 c          819 g         1597 t
ORIGIN      
        1 aaattaatat aaaaatcaaa aggatcataa acttttaaca agtgatgaaa tgggtggaaa
       61 ttgattctta tagacagttt gtctcataac attgaagtat tttgcaattt tagcattgaa
      121 acactttttt aatcttcaac gttgttctta taaacaagat gattgggaat cttttgaatt
      181 aacactgtat agcttagaac accctttata gaaaacattc gcttagcaat cttaataatt
      241 ccttgtgccc gatgtcgttt agattcagta ggttcctgct catcgatcat ggaaaaaatt
      301 tgaaggatca gatccttaat aaatttattt aagagaggat gatccattgc ttcatccata
      361 ctcgataaac tagtgataat gagctacacg tttttctttc aagttgatca atagcttcca
      421 cgataaaaat atctttagat aaaatcaaga accgtaggaa ctacagggat atttggtcaa
      481 taagagccac cgctattagt aaaaaataag ctatcaagta tgctctcatc ccaagaattg
      541 cctacttcaa tcgttaaaaa ccgttaggtt tagctaagtg ggggtagttc actcaatact
      601 tttatcaata atagagcgca aaactttttc tcttaattca actgaatcaa tattattgtt
      661 ttgttcataa ccatgaacta atagatcaca aatataaagt tgtgatattt ttccagctaa
      721 agatccacca ttaagaaatt cttcaatggc tgtttgtaaa actaagtctg cagactttcc
      781 tataggtgaa tgaatatagt tagttatagc aataattttt gcgccatttt ttttagctat
      841 tttcaatgaa tcataagtat ctttcgtttt cccagataaa gaaaaaatga ttactaaatc
      901 attaactgtt aataaagaag cgacttgggc ttgaaaatga gggtctagta cagcttttgc
      961 ttgaactcct acgcgaagaa acatattttc taaatcaaga ctagtatttc cactagatcc
     1021 cactccaaat atatatatgt tttttgattg agtgatcatt ttaatggctt cattcaattt
     1081 ttcttcgtct aaaagcctaa tagtggaata gagagcttct gttaaactat tagcaatttc
     1141 atcatagtac tttacactag tcggatgttc ttttttttga ctaaagtctt cctttgcaat
     1201 ttcaattttt aaatcagaaa aaccagaaaa accaattttt tgacaaaagc gaataatagt
     1261 agcatcgcca acttttgtgt ttttcttaat atcactcatc gtactataaa taatagtttt
     1321 cccagaattt aaaataaaat cagcgacttt tttttcagac ttcgttaaaa atggataatt
     1381 ttctttaatt aaatagatat aactcataca aactcctcta caatttcaac ttttctccat
     1441 tatactccaa aataataaaa aaaactccta aatactccat ttttatcttt acttttatga
     1501 aagcggatgc tatattgttt ttggagttgt tatccaaaaa taataaaaaa ttggagtgtt
     1561 taggataatg gataaaaaga ggtttttaga tcttatttca aaaggtttga ttgtttcctg
     1621 tcaagcatta cctggtgaac cattttacaa agaggaagaa agtttaatgc cttattttgc
     1681 tttggctgct caaagggctg gagcagttgg tatacgtgca aattctgttc gagatattat
     1741 tgaaattaga aagtgtgtat cattaccaat gattggaatt attaaaagag attatccacc
     1801 agaatctcca ttcattactg ccactatgag agaagtagat gaattagtag aaaccggtgt
     1861 tgaggtaatc gctcttgatt gcactcttag aaatcgacat gatggactat ctataaattc
     1921 ttttattact aaaataaaag aaaaatatcc tcatcagtta tttatggcag atatttctac
     1981 atttgaagaa ggcaaaaatg catataaagc aggaatagat tttgtaggaa ctactttaag
     2041 tggatatact gaggaaagta aaaaacaaga tgggccagat atagatttga ttaaaaaatt
     2101 ggtagaagaa gggatttgtg taattgctga gggaaggatt catactccag aacaagcaaa
     2161 agagataaaa aaaataggag ttgcaggtat tgttgtgggt ggtgctatta ctcgaccaca
     2221 ggaaattgca tcaagatttg ttaaagcatt aagttagggg gagaaataaa atgtttaaaa
     2281 aattttcaca attaggacgc gctttcatgt taccaattgc tatattacca gtagccggat
     2341 tactattggg actgggagga gcgttaacaa atgagagtgc tataaatgct tatccatttt
     2401 tagatcaacc gtggttgcat acaattttaa gtattatgag ttatgctgga aatgcagttt
     2461 ttgcaaacct agcactaatt tttgcaattg gtattgctgt aggacttgca aatggagata
     2521 aaggaacagc tggtttagct ggtggagtag cttatctagt atatactgca actattagtg
     2581 ggtttcttga acttttttca gcaaaagatg caacactaga tacaggtgta gtgggatcaa
     2641 ttgttgttgg tggtactgtt gcatttttac acaatcgtta tagaaaaatt gaattaccac
     2701 aattcttagg tttttttgga ggatcaagat ttattccgat tgtttctagt ttagctgcaa
     2761 tcattattgg ttctttcttt tatttaattt ggccaccaat tcaaaatggt ttagtagttg
     2821 ctggtgaaca aattgcccaa atgggtagtt taggaacttt tttatatggt tttttattgc
     2881 gtttaacagg tgctgttgga ctacaccaca ccatttaccc tttgttctgg tatacatctt
     2941 taggtggtac cgaaactgta gctggatcaa caattgctgg agcccaaaat attttctttg
     3001 cccaactagc tgatccaaac cacactggtc tgtttactta tggtactaga ttttttgcag
     3061 gaagatttgc aacaatgatg tttggattac cagcagcatg ttatgcaatg tatcgtgcaa
     3121 ttcctaagaa aaatcgtaaa aagaatggtg ggctttattt tagcggggcc cttacatcat
     3181 ttttaactgg aattactgaa ccagttgaat atatgttttt atttgttgct ccttggcttt
     3241 atgtgattca tgcattctta gatggattat ctttttattt tgctgatatc ttaaatattc
     3301 gtgtagggaa ttccttttca ggtggtttga tcgacttttt attattcggt gtacttcaag
     3361 ggaatgacaa aactaattgg ttaaaattaa ttccttttgg tattgcatgg gcatttatat
     3421 atttctttgt ttttacattc tttatcaaaa aatttaaggt agctattcct ggaatggaag
     3481 aagaatcaga agttcattct gaaaaagtaa taaacaaaaa tacatcatta catgaagagt
     3541 ctcttatcat tattgaagct cttggtggaa aagaaaatat tgagtctgtt gaagcttgtg
     3601 caactcgttt gcgtgttgct gttacgaatg gggagattat tcaaaaacaa acaataaaag
     3661 acactggtgc aactgctgtc ttcgaagtga agggtggcat ccaagctgtt tttggaggga
     3721 aagctgactt attaagtcaa gaaatcaatc aaatattagg aaaagataat taggaggttt
     3781 ttaatatgtt tgaaattttc aaaaagaaaa aacaagaaac tttttatgca ccttgttccg
     3841 gaaaaatcat ttccatcaca gaagtaaatg atcctgtctt ttcacaaaaa ttactaggtg
     3901 atggttttgc cgtcatacct gatggttcaa aaatttatgc tccattggct ggtcgagtaa
     3961 caaatatatt tcctactaaa catgctatta atataaaaag caactcaggc attgattatt
     4021 tgattcatat tggaattgat acagttgaat tacaaggaga gcctttttcg atttgtgttc
     4081 aagaaaatga tgaggttact cctgaaacct cgttggttga tgtggatttt gacaaaataa
     4141 taaaatctgg taaagatact tctgttatag ttgtttttcc ggagaaaaat cagttcaatg
     4201 atttaataat agataatggt cgaattgctc atgggaattc atgtggaaaa atcacttttt
     4261 aaaaaagtgt atataaaaag ctataaaata agctaaatag gatatttgtc aaatgaagtt
     4321 ggtgaacaga aagaactgtt ttctttctgt tcattttttt attttattag taatgattcc
     4381 ccatgccaaa tcatgatccg taatttaaag ttataaaagc ttcgaaatcc attggcattt
     4441 cgttggaata ccttgatatg attattcaaa tataaaatat agagagaaga gagagaggga
     4501 agatatttat aaagggcaaa gttatgttta ctcaacttag gaaatagaaa tagatcaatt
     4561 tatattctct tgtcaatcga atatgaacct tagcactata taccaaatat agtaaaatgc
     4621 tgaaagatg
//
LOCUS       DACCCS010000118         4623 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.128, whole genome shotgun sequence.
ACCESSION   DACCCS010000118 DACCCS010000000
VERSION     DACCCS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.128"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(72..542)
                     /locus_tag="IVK82_002751"
     CDS             complement(72..542)
                     /locus_tag="IVK82_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP6393754.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            872..2512
                     /locus_tag="IVK82_002752"
     CDS             872..2512
                     /locus_tag="IVK82_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP6393755.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2666..3988
                     /locus_tag="IVK82_002753"
     CDS             2666..3988
                     /locus_tag="IVK82_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP6393756.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4056..4487)
                     /locus_tag="IVK82_002754"
     CDS             complement(4056..4487)
                     /locus_tag="IVK82_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6393757.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEA
                     KKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
BASE COUNT         1325 a          894 c          862 g         1542 t
ORIGIN      
        1 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
       61 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      121 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      181 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      241 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      301 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      361 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      421 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      481 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      541 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      601 tcctatcaac gctatcatgt gctttttttc tcagaaatcc tatttatcca ttccatctat
      661 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      721 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      781 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      841 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
      901 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
      961 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1021 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1081 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1141 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1201 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1261 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1321 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1381 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1441 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1501 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1561 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1621 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1681 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1741 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1801 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1861 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     1921 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     1981 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2041 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2101 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2161 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2221 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2281 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2341 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2401 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2461 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2521 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2581 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2641 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2701 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2761 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2821 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     2881 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     2941 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3001 cagcaatatg ggctttttgg acttttatat tgctgccttg attactggta gcatccttgg
     3061 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3121 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3181 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3241 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3301 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3361 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3421 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3481 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3541 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3601 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3661 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3721 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3781 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3841 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     3901 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     3961 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4021 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4081 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4141 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc tacgccagtc
     4201 atcaaagcct ttccgatgcc tcttttttca gcatatttag acacagcaag tgccataata
     4261 ttgaacattg gatcgctaaa aatcgattca taggactccg cttgtacata gccagctgct
     4321 tttttagaag taggatcttc ataaaccaag aaaaaatttt tctctggctc cttcagcagt
     4381 ttcttcattt gtctcaccgt aacggttaat ggtatatcat accctaattg atccgcattg
     4441 atttcacgta gtgcttctgc atcttctaaa cgcatttccc gtaacatcca aatcaccagc
     4501 caatttctga ttttttaaaa tgaatttttt tatcatagta tactcttttt ttcatttaga
     4561 acaatatgtt tcagtgattt tgacttgcgt tagtctagat tctcctaatg tttgtaagat
     4621 gac
//
LOCUS       DACCCS010000119         4616 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.129, whole genome shotgun sequence.
ACCESSION   DACCCS010000119 DACCCS010000000
VERSION     DACCCS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.129"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1476
                     /locus_tag="IVK82_002755"
     CDS             <1..1476
                     /locus_tag="IVK82_002755"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP6393758.1"
                     /translation="RLLEQVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATI
                     VDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKK
                     LGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPH
                     MVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTY
                     NLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLD
                     DSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLE
                     DGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEEN
                     IQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIK
                     KIRQEQQVLEKELLEAISSLQTTPENEMWLQGALEVFKHEQ"
     gene            1466..2665
                     /locus_tag="IVK82_002756"
     CDS             1466..2665
                     /locus_tag="IVK82_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP6393759.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            2678..>4616
                     /locus_tag="IVK82_002757"
     CDS             2678..>4616
                     /locus_tag="IVK82_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP6393760.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSS"
BASE COUNT         1615 a          790 c          969 g         1242 t
ORIGIN      
        1 cgattgttgg aacaagtggt tcttttagca gatgaatcct tagaggaata tgacacagta
       61 agcaaacaaa caatgttgta tcgtgaatta ctttcagatg aagaatcaaa agaagatctg
      121 attgccacaa tcgttgatat tttgggatat gcaattgcac cagagtatct tttcaacgtc
      181 ttagcggatc aagcaaaaca agcaacgttc caattgaacg atctaaacaa agcctttgtg
      241 caattggcat ctacctacaa tcaatttaat gggttatttg atgatgtgga tctccaatca
      301 aaaaaactag gaacagatga gcaacaacga aatgtgacga ttaccgaagt catcaagaaa
      361 ttgaatgatg tagatgtttt agaacacgat ggggacgtga tcggggatgc gtatgagttt
      421 ctgatcagtc aatttgcttc agaagcaggg aagaaagccg gagaatttta cacacctcat
      481 atggtttctg atatgatggc gcaaatcgta acgctcgatc aaaaggaacg acgattcttt
      541 agtgtttttg atccaacgat gggttcaggt tcattgatgt taaacgtacg taactactta
      601 acacacccag ataacgttaa atatcatggg caagaactaa atacaacgac atacaatcta
      661 gcaaaaatga acttgatttt acatggtgtc gacgccgaag aaatgaatct ccgcaatggg
      721 gatacgttga ataaggactg gccaacagat gaaccttaca catttgatgc agtagtaatg
      781 aacccacctt attcggctaa ttggtcagca gatacgactt tcttagatga ttctcggttt
      841 aatcgttatg ggaaattagc gccaaaatcc aaagcagatt ttgcgtttct tcttcatggg
      901 ttctatcatt tgaaggaaac aggtaccatg gccatcgtct taccacacgg agttttattc
      961 cgtggcgctg cagaaggggt catccgtcaa aaattactag aagatggcag tatttatgct
     1021 gtgatcggta tgcctgcgaa cttgttcttt gggacatcga ttccaacgac ggtgattgtg
     1081 ttgaagaaga atcgtcaaac tcgagatgta ttattcattg atgctagtcg tgaatttgtt
     1141 aaagggaaaa atcaaaataa actatcggaa gaaaacattc aaaaaatcct cgaaacctac
     1201 gctgaaagaa aagatgtcga gaaatacgct catttggcaa cctttgatga aatcaaagaa
     1261 aatgactata acttaaatat cccacgctat gtagatactt tcgaagaaga agaaccaatt
     1321 gatatggttc atgtcggaaa tgacatcaag aagatacgac aagaacagca agtgctagaa
     1381 aaagaacttt tagaagctat ctcttccctg caaacaacac ctgaaaacga aatgtggtta
     1441 caaggtgcgc tggaggtgtt caaacatgag caatgataca cgaccagaaa tccgtttccc
     1501 aggatttacg gaagattggg aagagcgtaa gttaattgat ttagttgtta gacttaataa
     1561 aagtacaaac tccagtagac ttccaaaagt tgaatttgaa gatatcgtgg caggagaagg
     1621 tagactgaat aaagatgtga gccagaaatt tgataataga aaagggattg aatttttacc
     1681 aaatgatatt ctatatggaa agttacgtcc atatttaaaa aattggctca aggcaacttt
     1741 tacaggcgta gcgattgggg atttctgggt ttttagagct aagaagagcg actcggactt
     1801 tatttattct cttattcaag ctgatagata tcaaaaggct gcaaatgata catctggaac
     1861 aaaaatgcct cgttcagact ggaaaaaagt ttcagataca gtattctata ttcccaatga
     1921 tttaaaagaa caacaaaaaa tcggcatatt cttcaaaaaa ctcgacgaca ctatcgctct
     1981 tcatcaacgt aagttagatt tgctgaagga aacgaaaaaa ggattcttac aaaaaatgtt
     2041 ccctgaaaac ggagcaaaag tgccagaaat ccggtttcct ggttttacgg aagattggga
     2101 agagcgtaag ttgggagaat taggtaaaac tcaatcggga attggatttc ctgatgccga
     2161 gcaaggtggt tctgaaggta ctccattttt taaagtttcc gacatgaata acattggtaa
     2221 tgagtatgaa atgagaaatg caaatcatta tgtatctaat gagcaaattg agcgtaaaaa
     2281 atggaagcca ataaaagatg tgccagcgat tatatttgct aaagttggcg cagcaataat
     2341 gcttaataga aaacgtttgg tcacaagccc tttcttaata gataacaata ccatggctta
     2401 tttatttaat aatacttggg atatctattt tggtaaaata ttgtttgaaa ctataaattt
     2461 gccaagatac tcccaagtag gggcattgcc ttcttataac agttctgata ttgagaatat
     2521 cagtgttaag gttcctgtaa aggatgaaca acaaaaaatc ggtactttct tcaaacagct
     2581 agatgatacg atcgctcttc atcaacgtaa gttagattta ctaaaagaaa cgaaaaaaag
     2641 cttcttacaa aagatgtttg tctaggaggg aagaagaatg aaaattagtc accgtgagga
     2701 agcagaagtc gaagaacagt tgattcgtgt acttggagaa gggcataatc agtggacgta
     2761 tcgtccggat ctaaaatcag aagaagacct ttgggtcaat ttacgccaaa aaattatctc
     2821 aaataatcaa gcagaactga atgattttcc tttgaccgat aaagaatttg aaacgatcaa
     2881 aacggaatta ctgctgcgaa cgaaaacccc atttgatgct gcgaaatggt taaaaggtga
     2941 aaatgggatg gctcggatca cgattgaacg ggaagattcg caattgggtt ctgtctcact
     3001 tatcctttat tccaatcaag acattggtgg aggaatctct acttatgaag tggtccatca
     3061 aattgcaaaa cgtggaagta acatagaagc acgggatcga cgttttgacg tcaccttatt
     3121 aatcaatgga ttaccgattg tccaaatcga gctgaaacaa gtaactgcaa aagatggtgt
     3181 ttatcaggca tttaaccaaa tcaaaaaata tgcagaagaa ggtatgtttc ggaacaatat
     3241 cttttcgacc ttacagttgt ttgtggtttc caatgaacaa accactcggt attttgcaaa
     3301 tgccttaccg aaagacttgc accctaagtt tttatttagt tggcgaacga aagacaatga
     3361 aaaagtagaa aacttatatg agttttgtaa acaagtgtta aatatcccgg atgctcatcg
     3421 tttgattgcg gattatacaa tcgtcagtga agatcaagat aataaaaccc tcatggtgtt
     3481 acatccttat caagtacatg cgattcaagc cttgtttatt gccgcaaata aacatcaatc
     3541 agggtatgtg tggcatgcga ctggttcagg aaagacgttg actagttttg tatctactaa
     3601 gcttttagca cgaaaatcgg ggatcgatcg aacgatcatg ttagtggacc gcaaagattt
     3661 agataatcaa accaccacag agtttacaaa atttgcttcg gaatttaata ctgggatttc
     3721 ttccgggaac gccaaagcga atagcttgat cgttggaacg ggaagtgcca aagaattaag
     3781 tgaaaccctg ttggcagatg ccaatgcaaa tgttgtaatc attacaaccc gtcaaaaact
     3841 agatgcggcg ttgaaatatg caaagaaaca agaagagaaa aaaggaacca atcgcttcca
     3901 aaaattaatg ggccaacata tcgtgtttgt agtagatgag tgtcatcgag cgttaagtgc
     3961 tgaaaatatg gaagaaatca aaaaaatgtt tccgaaatcc acttggtttg gctttacagg
     4021 tacaccgatt tttcctgaga atcggaaaca agccaaaggg caattggcac gtacaaccca
     4081 tgatcagtat ggagaggtct tgcataccta taccattaag aatgcgctag aagatggctc
     4141 tgtcttagga tttcaagtag agcatgaaaa caccgtggag ccgacttcac tagagaataa
     4201 aatctatcgg aaattaaaag aagtagaaac atatgcagag tattcatcag aacagatcaa
     4261 tcgtatgatt gatcaaatgg aacctgtcaa aaaagaaagt tatttggacc cagctgtttt
     4321 tgaagcagat gaacatatcc aaaaagtcat tcataaaatg ttccgtccag ataatgccta
     4381 tacaaaattt gattttcgaa atggcaggcc aacgaaatct gcaatcttaa ccacgagttc
     4441 cattgacatg gccaaaaagt attatcgggc catcaaagag atgacgaaag agcctgattg
     4501 gttaacaaaa gagtttccca atcagctcat tcgtgaagga cgaacgatgg aggatcctga
     4561 ttttccacga atcgctatta cctattcatt agaagaaaat agccgagatt caagtg
//
LOCUS       DACCCS010000120         4615 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.130, whole genome shotgun sequence.
ACCESSION   DACCCS010000120 DACCCS010000000
VERSION     DACCCS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.130"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(15..962)
                     /locus_tag="IVK82_002758"
     CDS             complement(15..962)
                     /locus_tag="IVK82_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP6393761.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1140..1943)
                     /locus_tag="IVK82_002759"
     CDS             complement(1140..1943)
                     /locus_tag="IVK82_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP6393762.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2089..3003
                     /locus_tag="IVK82_002760"
     CDS             2089..3003
                     /locus_tag="IVK82_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP6393763.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3051..3521
                     /locus_tag="IVK82_002761"
     CDS             3051..3521
                     /locus_tag="IVK82_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP6393764.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3606..4214
                     /locus_tag="IVK82_002762"
     CDS             3606..4214
                     /locus_tag="IVK82_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP6393765.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4348..4548
                     /locus_tag="IVK82_002763"
     CDS             4348..4548
                     /locus_tag="IVK82_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6393766.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1511 a          716 c          911 g         1477 t
ORIGIN      
        1 attgttttca gctattaata tcctgcagac aattttactt gattggtttg aagctcatgg
       61 gtggcaaagt agcttttcag attggttaaa aaaatagcca ttaatttctt ttgaaaatca
      121 ggtgtcaaac ctgcaatgtg ggaagtaatc agtacattgt ccattttcca aagaggatga
      181 tcttgtggaa gaggctcttc ctcaaaaacg tctaatgctg caaaggaaag ttgctggtta
      241 tttaaagcct gcaccagatc ttgtgtctta acagaagcac cacggccaac attgatgaat
      301 acagattttg gatcaaagtg ttcaaacata tgctgatcaa agagcccagt tgtttcctcc
      361 gttagaggta agatattgac tacgaaatct acttcaggaa gaacttcctt tagttgattt
      421 aaagaaaaag tcttttccat tccttctgct ggatgaccgg atgtattgat tccatagcag
      481 ttcatgtcta atgctttgca tttttttgca atcatttttc caatatggcc agttccaatg
      541 attagtattt ttttcccttg gatagattgg taatggaccg atgaacctaa ccatttcatg
      601 tcactctgtg ccttttgtgc ttgaaaaaga ccacgagaat acccaaggat gattcctaag
      661 acatgctccg taatcgattc actgtggatg ccactaccat ttgaaagtaa aatatgtttc
      721 tttgaaaaag tctcaagggg tagctgatca acaccagctg agatagattg gacccatttc
      781 aaggtagaag aggctaatag tttttcttgg tagtctgagt tccagcccaa actgatctct
      841 acattttgta gatcctctgg ttgtaattct gttttaaaga tataaccagg cgctatagta
      901 tggatttctt cgataaattc atcacgaaat ggacgatctg cataaatgat tctttctgac
      961 atagttaatt tcccgccttt ctttactcta agtgtagcgc atacaagaag aactttgaag
     1021 cgccttgctt gttttttgag aaagaaatat ttttcaaata aaaaaggggc tgtgacaaaa
     1081 aaatgtcaca acctctaaaa gggtctattg aagtagaaat aacgagttgt ctttatttct
     1141 taattgatcg atatttttgc ccatcctttg acagcttgag caaaaataaa gattcctgca
     1201 aaagcaatga aggcgctagt gtcaaaagaa ggaagaaaga ccatcaagat tgttgcaatt
     1261 atagtaggca atgaaattga atagatcaat gtttttaaac aatcaagaaa agtagctttt
     1321 gttagtcgaa agcgtgcata gagaaaagca gcaatgttgg taaatagcaa agtgatcagc
     1381 agattcagaa aactaggata aatagaaaat ataaatgtca ggacttttat ccaaagtggt
     1441 atagatgctt cacttaaaaa agaacggagc tgcttacctg ttaagttttt taaagattca
     1501 ttggtgtagg gaagttctaa ttggttattg ttcaacaaag cagaagtcgt gcctgtatta
     1561 ggaagagcta tgataagttt gttttttaac atcccgacac taacaaaatt ccccatcaaa
     1621 tcagtagaga tgtcctgttc tgttcgtttt ccctctggat caaatgtgaa gataatggag
     1681 tttgtttggt aaatgaatcc ttctttttct tcggtttgaa gctgcccgtt ttggattgta
     1741 aaatcaggaa tgcgagtagc aatcttttgc ccatccaatt gaatgttgtt taagacttga
     1801 aaaacttgaa aagagattgg caaagccata attatactca aaactaaaag atagacaatg
     1861 gctttccaaa atgggacatt tctcgcctcc tttaggtctt tgaatcgtat aaaggaactg
     1921 ataattaatt gttttgttgt cataagtact cctcatctag tagtgtcatc acttgcaatt
     1981 ctagcgaagt atcaagtgaa atacaagtgc tttaaggaaa cagctgaatg attggtgaaa
     2041 tatcaaaaaa atgttattct tgtctaaaga ctatccaata taaataaaat ggaggaagga
     2101 atagtgccta cacagccaca gagatcatca aaaaaactat ttggagtagt ggttttgaat
     2161 gcggtgatca ctttatcaga atttattgga gggattatgt cagggagttt gtctcttgtt
     2221 tcagatgctt tccataacct tagtgataca ttggcaatta ttttttctta cggggcacaa
     2281 aaaatggccc gaaaagaagc aaataaaaaa cgtacgtatg gctaccagcg tttagaaata
     2341 ttgagtgctt tcatcaactc attcatattg atcatattat ccttgttttt aaccgttgaa
     2401 gcattcaagc ggttcacttc accagaaaag ataaatagtc atttgatgct aacagtagca
     2461 gttattggat tgctcgcgaa tttgttctca gtattattat tacgacaaga agcagatgaa
     2521 agtttgaata taaaatctag ttatttgcat ttgttaagtg atacactgtc ttcggtaagt
     2581 gtgattatcg gagcaatttt gatccgtttt ttcgatatct actggatcga tccagttatt
     2641 acactgatca tttcgatata tatattgata gaagcgataa tggtgatcaa aaaagctgcc
     2701 gctattttga tacaaagtgc acctgctatc gactatgaaa aaatggaaca agaaattgaa
     2761 gcaatcgatg gggtaaagga tgttcatcat gtccatattt ggcagtatag cgaaaagata
     2821 attattttcg acggacatat tgattttgaa gatcagctac taagcgagat tgaaaagata
     2881 tatcctcgta taatttcttt attaaaatta aaatatggta ttacacatgt gacgatccaa
     2941 gcagagacaa aggcaaagga tcaaaaaaaa ttaatttttt taaataaaga tgaaagttat
     3001 tgaaatatag agtatgataa ataagaattt ttaataaggt aggttggctc atgaagcagt
     3061 atcgacagtt aaaaggatat ttagaagcaa aaggatattg ggagatacta gtctatctct
     3121 tttttggtgg tttggctacg attgtaaatt ttgttagttt tgctctcgca cgccaatatt
     3181 ttgatttaag tatggcctta tccaattcta tttcgtggtt ttgttctgta ttgtttgctt
     3241 ttgtaacgaa caaactttgg gtattccatt caaaatcacc gaattttaca catgcactta
     3301 ttgaatgcgg taagttcttc ttctaccgta tcctttctta cggattggac atgggggcaa
     3361 tggtattgtt gatcaatgtt atgaacagca atgagtatgt ggcgaaaatc atcactcaaa
     3421 ttattgttat ccttgcgaac tatattttca gcaagttgtt tatttttaaa gaaactgagg
     3481 tttttgaaga agaagtaggt caaagtgaaa aagacgacta ggcataacaa ttgaagaatt
     3541 tccttctctt tgttactctt tgtaatgtag acattattaa gaaaacattg aggaggaatt
     3601 tttttatgac ttacacatta ccagatctac cttatgcata tgacgcattg gaaccttata
     3661 ttgacgaaga aacgatgcat ctgcatcatg ataagcatca caatacttat gtgacgaatt
     3721 taaattcagc aattgagaaa tacccagaat taggcgaaaa aacaatagaa gaattattat
     3781 ctgatatgga cgctattcca acagatatca agacagctgt acgtaacaat ggtggcggac
     3841 atgctaacca ttcatttttc tgggaaatca tggcaccaaa tgctggtggc gaacctacag
     3901 gagaaataaa agaagcgatt aatgaagctt ttggtgattt ttcttctttt aaagaagaat
     3961 tcaaaaaagc agccgctgga cgatttggtt ctggatgggc ttggcttgta atggaaaatg
     4021 gaaaattagc tattacctct actgcaaatc aagattctcc attgatggaa ggaaagacac
     4081 caattctagg tttggacgtt tgggaacatg cttattattt gaaatataaa aatgtccgac
     4141 cagattatat tgcagcattc tggaatgtta ttaattggga tgaagtaaac aaacgtttcg
     4201 aagcagcaaa ataaccattt cctccgtcat atcagtctta aaaataattt ggaaaataaa
     4261 agagcgaaag cttgctagcg ttttctaaat tgggtataat gttcttgcaa ggttacttgc
     4321 aagaactttt ttggaggata tacatttatg gaacacggaa cggttaaatg gttcaacaat
     4381 gaaaaaggat ttgggtttat tacagtagac ggcggagatg acgtatttgt tcatttctca
     4441 gcaattcaag gagacggctt caagagccta gaagaaggtc aagaagtaga attcacgatt
     4501 gttgaaggtg cacgcggacc tcaagctgct gaagtgacta aattataata atttgttgaa
     4561 taaagacacg acttcttaga gagtcgtgtc tttttgtcta tttatgaagt tttta
//
LOCUS       DACCCS010000121         4568 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.131, whole genome shotgun sequence.
ACCESSION   DACCCS010000121 DACCCS010000000
VERSION     DACCCS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.131"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(52..1254)
                     /locus_tag="IVK82_002764"
     CDS             complement(52..1254)
                     /locus_tag="IVK82_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393767.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(1241..3028)
                     /locus_tag="IVK82_002765"
     CDS             complement(1241..3028)
                     /locus_tag="IVK82_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393768.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(3041..3994)
                     /locus_tag="IVK82_002766"
     CDS             complement(3041..3994)
                     /locus_tag="IVK82_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6393769.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(4179..4529)
                     /locus_tag="IVK82_002767"
     CDS             complement(4179..4529)
                     /locus_tag="IVK82_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6393770.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
BASE COUNT         1595 a          841 c          773 g         1359 t
ORIGIN      
        1 tctgacagct ggagttttta tgtttaaaaa tgaattagta catcggtatt cttaaaagta
       61 atttagttcg tcttttgatt tctcaggagg gatcaaaaag gcgaaagcac taagtatagc
      121 aaaacttgtg atgatttgcg tacgatacgt aaaccaataa tgaatctgtg agtgattagc
      181 taaaatattg taccaaatat acggcgttac agcagctaaa acaaacaaaa ctagaagttg
      241 gaacggacgc cagctatttc gtttacgata agcaaaatag agaaaaacaa gacaagtaat
      301 caatagcaat aatattgcta cttttggata catcaatcta atattgcttt tcagcatctc
      361 taatcgatgc aacgggtatt tgtcgtttcc ttccgttcga aagaggattt ggttgattgc
      421 atcttttaca atgtttttgt tcaatatgat cgatgatagg atccatttag ccacccatgt
      481 caaaccatag cctaatcccc aaaagatacc ggtaaaaaca aaactgccta cttgtttgct
      541 caatgaactg cgtttatcca ttaaatactt atttgttaca taataaataa tgatcacagg
      601 tatcccccaa gagatgaccg gtgttgtcaa caagtcaaaa aagttagcta aagaaccaat
      661 gacaaagaaa tacaagaata attgttggtt ggtgttggag agttgtggtt ttgttaaaaa
      721 tagtaataca gacaataaag tcaaaatcca aatatttgaa aattgcatac tgaagaaaaa
      781 ggttgcagca tttcctatag caagcgaaat caacaaggct gtcgttaaat agatgtcgct
      841 acacttggtt aaaaagtagc tcgtcaatcc aatcagcaga aaaagaatgg ctgcgtaaat
      901 cattcgaata ctccccaaac tcatccctag cagtaaaggt cgtaaaaaaa gctgataacc
      961 atgccaataa cggggataat ccgccataga catcgcgttc tccaaggagt tcaattcagg
     1021 atctgctttt gtttttcgta tcatcaaatg atcagtaaaa ttatctaacc gagtgcctag
     1081 catatttcct tgattgatag aaggatacat tccttctttt tcaaccatct gtagagattt
     1141 ttctgcattc gattcaatag agtgctgcgg aatgaaaaaa gtaattgtca gtagtacgca
     1201 atagctagcg atcaagagaa caaaagcaaa aaatatcttt ttaaatagtt tcatgagtat
     1261 ctcctatatc tgttttcatt ttttctcttt tatctagtaa aaaaggagag aggatataaa
     1321 caggtagacc taatgctaaa acaaacacat accgtaaact aaaagccaca ggagtagcaa
     1381 tcataatagt agcaaaaaca agcataccgg gaagaaaaag gagtaaatac cgatattttt
     1441 tcttaaagat tgctaaaagt gcactgccga tagctaacca cagtaatgtg ccggaaccaa
     1501 agaaatcacg agcatcgatg atttttttca ttcctgacaa tccagtaaga tgttcaatca
     1561 aatctacttg cttcatacca taattattcg tgtttacacg agtatctaaa aagccgtaac
     1621 tattttttgt accaggtacc cagaacccga acgtttctaa aatataagca tctgtataga
     1681 tttttatatt acttggtaaa tttcttgccc atagtgatag aaattctttt ttatgtgtat
     1741 tcaaaaactc attgttaaaa tttttatgcc acttgattga gtctgcaaga ctaggagaat
     1801 acgcttcata atcttttatc gggagtagct gattcatgaa tgctttatct ttcttagaca
     1861 agtcaccatc cataacaata gttctagaaa tttgctgcaa aggaacacct aattgttcag
     1921 cagtttctgt aggaatagca taacgagcca tcaacgggtg gatgagtagt ccagcaatca
     1981 ctgtataacc cagcaccact tgaaagaatg cttgatacct tttttgtttc aaaataatca
     2041 agccgcaaat caaacctgtg ccggttacta cataaatccc attatttctt agataggtgg
     2101 tcagtaaagc aaggatgatt acgtaaatct gattgagaat cgttggcttt tctcgttgaa
     2161 aaataaagtc aaaaaccgct aaggaaaata aaaagcaagc gatagagaac aaagtatctt
     2221 tttgcatgtt caaggcgtag attggaaaaa ccggtgcaaa tgcaaaatag cataaactaa
     2281 tgagtaccgg aaaaaaatgg atgtttcttt ttaacagcca gcaaagagaa taacttatcg
     2341 ctgaagctgt tatcaatgtt tgaaaaatcg aaaataaaaa tacaccgtga ttgatcgtat
     2401 cagttggtaa atcatgaaca aatagcttga ccagtaaggt gaataagact ggatgatgat
     2461 tgcttatagg aacatcaccc atagattggg cgaccgaact taacgaatca ggcaaaacca
     2521 ctccaggata caatgccaac agataaggaa gccaacacag aactaaaaag acagtaaaga
     2581 ataaaaaata ttttcttgaa gagaaagcct gtgtgttttg agcagtaaaa aatgtttcca
     2641 gttttggcat tgataaataa atcgattgta aaaataaaaa agaatacaca aaaatggaga
     2701 aataaacaag aaagtaaaaa atacttccat cagcaatcgt tgtctttgta tagtttgctg
     2761 agacatactg aaattgtatc tgtctgccaa tagaaagtgt aagagcaaaa gctgcagaaa
     2821 aaaacaatcg ggcaatagct gcaactttgt tttttattga aaacaactct attccatatt
     2881 gcttaaaagc aagtagcaaa accatggcta aaccaatgat tactgttgga ttcaaagttc
     2941 ctagaccata agaagtaaaa aaagcaaaac ctgacgcaaa taaagttgca aaaaaagtag
     3001 gaaatagatt ctcctttttc ttatgcataa aaaaggcctc ctacttcatt tctttatttt
     3061 gatattcgtg gtagacatgg tataaattca attcatattc tttcttggca tttgtgacga
     3121 ccgtatccaa aatcaaccct gttactaatg aaagaagggc taaagtcatc aatccagtac
     3181 ttaaaatcgc agaagggatg cgtgtaataa agcttgtcag catatattca cgaatcacag
     3241 gaatccccat gaccaatcca aataaaaaga agaagatggt ccagatccca aagaaaagca
     3301 aaggtttata atctttgcac attttgatga tcatcatgat cactttgaat ccgtcagaga
     3361 atgtattcaa ttttgattcg ctaccttcag gacgatcgcg ataatcgatt ggaatctcaa
     3421 cgaacttgaa ttttttatct aaagcatgaa tcgtcaattc tgtttcgatt tgaaaaccag
     3481 aactcataat agggaagctt ttgacaaaaa ttcgattgaa tcctcgatag ccggtcatga
     3541 catcgcgaat ttttgtttta tataagtaag taattgtatt tttaaccaga ttattcccaa
     3601 aatcatggaa aggacgctta ttttcatcaa aataggttcc atttgataga cgatcgccaa
     3661 tgaccatatc tgcttcgcca gaacgtaatt tctcaagcaa accatgtgca gcttctgcag
     3721 gataagtatc atcaccatct accatcaaat aataatcggc atcaatatca aaaaacattt
     3781 gacggattac attccctttt ccttgacgtg gttctttctt tacgattgct ccgccttgaa
     3841 cggccaattc gtatgttcga tcggtcgaat tattatcata gacataaata tctgcttctg
     3901 gtaattccct tttaaaatct tcaataacag ttgaaattgt cgcctcttcg ttataacaag
     3961 gaatgagcac ggcaattttt tcacgtgatt ccatttgtca ctctccttat ataataaaac
     4021 taatcttatt tcaataatac cgtataataa ctacaaatac tagctattcc aagaggaaat
     4081 agccctattt ttataaaaat cacccactat aaaagaagga taggatacct ctcttttaga
     4141 tagtgggtgc gtattcgtta tttcattttt ttgacgtatt aatctattgc gccctttttt
     4201 cttgaaatgt ctaatacggt tcctgtatga gcatctgcta aaaattcata ataaacagtt
     4261 tcgccatctt ctatccggga aagcccaccc cgataagcac tcgaccgaac ggcgaatttc
     4321 cgaataggtt ctttttcaaa agagatccaa gcaccttcga ttgcgccttc ttttaaaaaa
     4381 gatgttttga tattttcaag cacttggtct gctgataaca cttttttgcg atgataccaa
     4441 atcgttgaag ctaccccact caataatcct agaccgatcc caacggctaa cccgccttta
     4501 aaatagctta attcgctttc ttcatacatg tggatatacc tccttcttct cacttgctac
     4561 tctttgtg
//
LOCUS       DACCCS010000122         4199 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.132, whole genome shotgun sequence.
ACCESSION   DACCCS010000122 DACCCS010000000
VERSION     DACCCS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.132"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(2..1222)
                     /locus_tag="IVK82_002768"
     CDS             complement(2..1222)
                     /locus_tag="IVK82_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6393771.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1242..1847)
                     /locus_tag="IVK82_002769"
     CDS             complement(1242..1847)
                     /locus_tag="IVK82_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6393772.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1896..2828)
                     /locus_tag="IVK82_002770"
     CDS             complement(1896..2828)
                     /locus_tag="IVK82_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP6393773.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLSNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3253..4110)
                     /locus_tag="IVK82_002771"
     CDS             complement(3253..4110)
                     /locus_tag="IVK82_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6393774.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKNDTID"
BASE COUNT         1191 a          849 c          692 g         1467 t
ORIGIN      
        1 tttattttac agaagcactc gaaactgcat ctactgcagt ttcatcgtgt gtgctggcgc
       61 tggttactgc atctgttttt ccaccatgaa cagatgcact tgaaaccgca tcttcatttc
      121 caacaagttg ttgacccgtt tgttgtaaat accactcaat gattggtttg aaatcaagaa
      181 tgtattcatt gattccagca tactctccat tttcatcatg cattgcttgg taattatgca
      241 caatatattt ttctggcact tgttttggta catgaacacg taccgcatct gttgctccgc
      301 ttcgcagttg ttgaattacc cactctacat ttttatacga tttttctggg tgacagttgg
      361 ctaatggatt ccctacctga ccgggcacac gagcagctaa catctctttc gcttcttggc
      421 gcaaattata atataaaaat tggttattat tatctgcaaa agttaattcc atcggcatcg
      481 aatctaaaaa gtaattgatt tgatccactg tcaataaccc acgatttaat ttgacgtaag
      541 tatttccaga aacggcaccg actgcttcag aagctttttc caaccaatga tcatctgaca
      601 tatcaacatc gggtattgtc gtagcaattg ttcctgtcgc atgcaaatct tctggtggaa
      661 gctttttagg ccctgctaag gtagacacga ctttaatgaa ttttacaaaa cttgctaatg
      721 tactgcctaa aaagtcaaca gttcgtggat caatcaagcg atcttcttgg tcaaatgctt
      781 cttttgcttt tcccaataaa aattcatttc cgggcatcac taatgcattc acgcctggtg
      841 cgtccaaaat ttggcgcaag tgtaattgcg cgcgtgaagt tccttgatcg tagtaagaag
      901 cacccacaac cattacaggt ttattttcaa gtggatggat tttaaatgat agccattcaa
      961 gtgcactttt caatcctgct ggaaccgtat gattgtgttc tggcgtagca ataattaccc
     1021 catcagccat taagattttg cgatttagct gttgaatagc aggactattt gtttgatcat
     1081 catcttgatt gaacagaggt aaatctttga tttcaatgat ttctaaatta aatagttcat
     1141 gatattgttt ttggataaat gttaataatt tacggttata tgaaagttca gctgctgatc
     1201 cgacgatacc tattaatttc atctttgttt gctcccctcg attaaatcgt ttcccatgaa
     1261 aagctttcgg cttcacgttt atttgcttgg tgcgcatgtt gcaattgact agtgattgtg
     1321 acaaagagta agaaatcttt gaatagttca cgtaattttg cgacttgttc ttcatcaatg
     1381 agtcgtcctt gctcatcaaa ggcttggagt gaatgaccca ataaaaattc tgagcttggc
     1441 ataattcgtg cctttaactc tggtgaatcc aatatttggc gcaaatgtgc ttgtgcgcgt
     1501 gaagaaccta acgtgccata agacgctccg gtaatcatca ctggtttatc cacaaacgga
     1561 aaaataccat atgaaagcca atttaatgcg ttaatcaatg aagcaggaac cgcatggtca
     1621 tactctggtg tgctgatgat tacgccatct gcttgttcaa tttttccagc aatttcttta
     1681 acaacatttg gtagttctgt gttttcaggt ttgttaaata gtggcagctc cttgatttcg
     1741 acaatttcga tttctgcctc atttacaaaa taatttttca taaactgtaa taattcacgg
     1801 tttgttgacc gcgttgaatt tgttcctact aatccaataa atttcatctt cattctcttt
     1861 cctttctaac atcgatttct gactgttcgc acgtactaaa ttttttcaaa tccttttgta
     1921 tacaagaatt gattctcttt tgttacaact gctccctcga tacccggctg ttcttcaagc
     1981 gctttcaaca cttctaaaga agatgctcca aataaacgtg tagtccagat ttctccatct
     2041 acagaagaat cagacaaaat cgtcaggctt gctacgtcat ttttaactgg aaagccagtt
     2101 ttcggatcaa tgatgtgatg atacactgac gaaccatttt gtagataacg ttcgtagatt
     2161 ccagaggtaa ctaaagattg gttgcttagt ttaaccaccg caagcgactc ttcacgtgaa
     2221 cctttaggat ttcgcaaacc aatacgccaa gcaccatccg aacgtcttgg tgattcacca
     2281 aaaacaacga tatttcctcc taagttaact aaagcaaacg ctgcatttcg ctctcggcaa
     2341 tgtgtaatta atttatctgc aatgtaacct tttgctaacg cccccaagtc aatcatcatt
     2401 cctggtttgg ttaaaaaaac ggttttcatt ttttcatcta attgaatttg gtgtggatcc
     2461 gttagcagaa gtaactcagt tatctctgct tttgttggaa ccaatgcatc ttcaaatcca
     2521 atgtgccatt tttgtacaac cggaccaata gcaatattca gataactatt tcttgcacaa
     2581 ctatgtgctt ttcctaattt gatgagctca aaaagttcag gatgtacaat cacagcttgt
     2641 cgtccagcag cttgattgat ggccattaat tcagaagttg catcatgtgc gctgaagcga
     2701 tgttcataaa cgtgcaacca ttcgcctaac tctgctagta atcgactagg ctcttcatgc
     2761 ccaacttgta atgtaattgt tgtccccatc atttgccaga ttttcgtttc ttcgatcacc
     2821 tgattcattt tgtaccttct ttacttgaac tacggattac atgagtaatt agtttttatt
     2881 tttgttactg ttttcacaac cagatgatac gcctgctaga tttatttgtc aaccgatttt
     2941 atatattttt ttatataata aggtaatttt aatataaaat tttatattgt aaaaaaacaa
     3001 ataagggaaa caaacaacac atttttgtgt gttgcttatt tccctctaac tatcgttttc
     3061 tatttgatgg ttaacctact taaaataaat actgcaacga caaacggaga tcaagcatgc
     3121 tttgtaacgt attttacgag aattacgact ttattttatt actctatctt gctgccatac
     3181 ccgtctatcc ctaatcatct ttggtgaatt ttttcaaagt tagcataaca aaaataagta
     3241 ttttttcctt cgttaatcaa tagtatcatt ttttttcata tgatttaatt catcaattaa
     3301 ttttttgttt ttattaattt tagcaaaagc tagtgcatct tgaatcaatt cgagcacttc
     3361 tgccttcgac ttatttttag cctgtgcgtt cttcgctaat tgggcagcag ctcgtgctaa
     3421 gtaataagtc aaatggttat ccgaacaaat atcgattgcg taatttagta gtgcatcact
     3481 tgtttccaaa tcaccgtctt gcgcaaaaaa aacaccacaa tgaaagacga tatttaacac
     3541 ccgccagata tcttctgttg tacgaatcgg atacgtataa attttttcaa acactcgatt
     3601 gaaataaact tctgcttttt ccaactcttt ttctctagca tagaccattc caatacctgt
     3661 atatgccaat aaacgaaaca acgtgttggc tgtttctgcc atcaacaact gatcaaaatg
     3721 gaacaacaca tccgtaattg gtgcattcgt aaaaatcatt tcaaatcctt ttagatagta
     3781 gtaacgtgca atcagctcag actcgtcaat taccttggga tcaatggctg ctaaaatctg
     3841 tgtcaattgt tcatattcac tcgtaatcag tgaaaactct gctttttcta gctgttcatc
     3901 aagttgtgta ttgatcacgt tcatcgaaga aaaaagttca actaacggaa gattgattcg
     3961 attacataat ttgattaaaa tttttaacga agggacttga ccattgttct caaatttact
     4021 taatgttgct tgtgtacaaa ttccttcaga cacttctttt tgagataagt tggcagcctt
     4081 tcttgccgta ataaattttt caatgttcat tattttctct ccaatcagac ctcacacgaa
     4141 aatcatttct gtctcgattt tagcatgttt tctttgggaa tgctttaatt ggttaagat
//
LOCUS       DACCCS010000123         4157 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.133, whole genome shotgun sequence.
ACCESSION   DACCCS010000123 DACCCS010000000
VERSION     DACCCS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.133"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            179..775
                     /locus_tag="IVK82_002772"
     CDS             179..775
                     /locus_tag="IVK82_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP6393775.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IVK82_002773"
     CDS             899..2698
                     /locus_tag="IVK82_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP6393776.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IVK82_002774"
     CDS             complement(2976..3188)
                     /locus_tag="IVK82_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393777.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(4052..>4157)
                     /locus_tag="IVK82_002775"
     CDS             complement(4052..>4157)
                     /locus_tag="IVK82_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6393778.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1228 a          707 c          729 g         1493 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat aatcttaatt gtcaaattaa gttagacaga tcatcaatac
     4081 ttacgtaact caaaaatact tccaaaggtg ttcgataatc cagtgatttt ctaggaatat
     4141 tatttcgttt agatgcg
//
LOCUS       DACCCS010000124         4052 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.134, whole genome shotgun sequence.
ACCESSION   DACCCS010000124 DACCCS010000000
VERSION     DACCCS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.134"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..765)
                     /locus_tag="IVK82_002776"
     CDS             complement(<1..765)
                     /locus_tag="IVK82_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP6393779.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQT"
     gene            complement(987..1349)
                     /locus_tag="IVK82_002777"
     CDS             complement(987..1349)
                     /locus_tag="IVK82_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393780.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKH
                     FGFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNESDGLLGVIPSKK
                     SQMSESKFTSMFNDVSLR"
     gene            complement(1353..1670)
                     /locus_tag="IVK82_002778"
     CDS             complement(1353..1670)
                     /locus_tag="IVK82_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393781.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSTINVGLANSSLITDKFVYGKIAETEDESEKEFWLNILTYVMQLNQIGVIYRNKSKG
                     KEL"
     gene            complement(1771..2004)
                     /locus_tag="IVK82_002779"
     CDS             complement(1771..2004)
                     /locus_tag="IVK82_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393782.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(2001..2975)
                     /locus_tag="IVK82_002780"
     CDS             complement(2001..2975)
                     /locus_tag="IVK82_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP6393783.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVG
                     NLKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQ
                     LRRGKATDWHAMVYESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYK
                     YRNLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
     gene            complement(3424..>4052)
                     /locus_tag="IVK82_002781"
     CDS             complement(3424..>4052)
                     /locus_tag="IVK82_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP6393784.1"
                     /translation="SAEMRYISKQREKNEREKALVASEKAKIEEEKKSIIDLEKELAV
                     VFQKDPVPLNSFEKLSDGSYRLSSADFVDLYQKASNATNSKKYADMANESLGNTLDKN
                     IALFDKNMALEQENSFLKNQQKVDVSAVVAENKKLKEENRSLRRRLTQLKNAFQKSIT
                     RLSIRFGLVEKDMGISEELKILDQEKTLASQQNAPERNIGRSENEMEW"
BASE COUNT         1150 a          888 c          573 g         1441 t
ORIGIN      
        1 cgtttggcgc aattcagaaa tttcactcat gatttctttg tattcgtgaa tatctttaat
       61 tttgttggta ccgacaatat cccttttcca accgtaatca ttcattaatc gttctataga
      121 gtcaacttct tgatttctaa aattattgaa aagcactcga ccattatctt tatcagcgtg
      181 tatcccttgt tgaaccagtg ctttattaaa gctcggctgc ttttgaacac cccgtttata
      241 accttcagca accggcacaa tattcaagtg caggtgcgga cttatttcgt cattatgaat
      301 aacagcatta taaacagcca aattagggtt tctctcttga aactccttgt aatactgaac
      361 caaaatttta ttggcaatca ctcgattttg ttcagcttgt tgtttcgata tgccagtaga
      421 agaaccatct gctcgatttg tctggaaatc atctaaaatg cctatttgca caataaattc
      481 tcgttgaggt tccaactttt tatccttgcg cactttcgct aaatagtcct cgatttttct
      541 atcggctctt ttttgtttcg cattatattt ttctaccact tcgccaaaca attcctcata
      601 tttatctgct agattcgtct gctctaaata aatattttca tccgatttcg tgaagtcaat
      661 atgcttatgc caatcattgg ttttctggct atcagttaac tcacgattat tatgagaaat
      721 acttgtattc ttcgttcctt ttttaaatga aatactaaac cccaagttaa aaccctcctt
      781 cgcgctgtcg ctaataaaaa gagtgtaaac ctttctgtta ctctttgtca aagagtaaca
      841 ttttcctaat tgtgacatgg ctgtcacaat tccaaatgct ctccgagggt attcgctgtc
      901 acaagtgaca acgaaaaaag gagccgaaag cgtgtcacgc tctcaactcc ttttacgctt
      961 tattttgctt cacgcaaaat aatcgcttat cgtaaactca cgtcattaaa cattgacgta
     1021 aatttactct cactcatctg acttttttta ctcggtatca ctccgagtaa gccgtcagat
     1081 tcattgggat aatctcgttc gcttggcgct gatttttcat cagcaagcca ctcattatcc
     1141 actgattttt cgtgttgctg atcgtcagaa attgacgata accaccgctc aaaatcatca
     1201 aaagattcaa atccaaaatg cttccgaagt gccttaccca tttcagcatt ttccttctca
     1261 atcaatcgtt ttttctgttg acgaagtttt gcctgttctt ttttcaattt ttcttcttgt
     1321 gctttaatat ctgctaaagt cattcccaag atttacagct ccttgccttt tgatttattt
     1381 cgataaataa caccaatttg atttaattgc ataacataag ttaaaatatt cagccaaaat
     1441 tccttttcag attcatcttc tgtttcggct attttaccgt aaacaaattt atcagtaatc
     1501 aaagaagaat ttgctaggcc tacattgatt gtagaattga gaacgtccat cattctctca
     1561 agcatttcac tataattatc ttcatccggc aaattcaaat aatcagctaa tcgttcatac
     1621 atcaattcaa ttctatcaac tgtctcttct cttaaatcaa ttttatccat gttacacctc
     1681 atttccaaaa ctgccaccat ttccgattct ttaattttcg atacacctaa ttcatcagca
     1741 agatttttta tggtcatctt ttctgacaaa tcagtcatta gcttctccac tactttcagc
     1801 taccgcataa atatatttaa cggcgtgctt agggataata atcggagagt caattttatt
     1861 atttttcaaa atcaattcat attgcgttac atcaagtaac ttaccggaca tttttttgct
     1921 atccaaaaaa acaaccacaa cgtaatcacc tgttaaattt accaaccaat ttttcttacc
     1981 aaaatacttt tgtttttctg tcatttttct tcctcctctg ttacgtctag ccctagaagc
     2041 catgtgtcta attctttttt taaatcgtct gccgattctg caaaggtttc tcgtaaatga
     2101 ttcgtcaatt catcattttg actttcttgc ttcagcaaat ttcgatattt atattttgtc
     2161 tttcgattaa tttgccccca ataatcatgt tctgttgtta aagcagtaat catcattttg
     2221 tcagctacac ttgtatcagc aggcaagtaa tgaggactag caaaactatc taagctttca
     2281 taaaccatag catgccaatc agtcgctttc cctcgtctta attgtaattc taaccgccac
     2341 caactcacga tttcttttgg cacaattttt cttttcttct cttgttccag ttttttatta
     2401 tacatccgaa tttgacgttc agaagagcga gatccccaat atgccgtctc taattttcca
     2461 ttccgtccat aaataggctt aaaagaaact ggatcaacta cccgatattg tgtaataaac
     2521 tcatcaggta catcgataat gtcgcaagca atatctgcac gtgaaaagtg aggttcgata
     2581 aacaaatcat gaataaaatt tttcatggaa ctagctaaga attgactaat cttattcgga
     2641 ttgaagtcta tacgcccttt cccctcttgc cattttaaaa tttcaatata agcaatattc
     2701 tcttcatgtt ggtctaacaa ctgccaacca ttattactaa cagatcttaa ataaccgtta
     2761 ccttcattta ggcgcataag ctgttcaaaa tctaaaatca acacatcatg tgtagtgtga
     2821 taatagatat tctctttaag atttccaaca atggtaattc ggtcgattga ccactgaatt
     2881 tttagggggg tgtccataaa agggacactt aaccgcctgt tagagggggc tgttaatttt
     2941 tcccactcat ttatgatcgt gcctttttta ctcaaattta gttcctcctc aaagaaaaaa
     3001 gagattggtg gaaagaattt tccagagttc acgtaactct aaaaaattca taaccagcaa
     3061 tctctagtct tgaagaaacc tgaaattatt cgtataatga atttgagtta cggagctagt
     3121 gtgattagtt tccgtaaagg tcgagaacgt gtccagcgtt gctcgacttt atttttttgc
     3181 tcttttttca gtttatttat ttgataactc aataatagca cttctcttta gctatgcaaa
     3241 tatccaaaaa cataaaaccg caccctttga aaagggcgcc cccaaacttc gctaacgcta
     3301 agttggccaa aaaatggact tgccattttt tagcaaatgt cagcttcagg gattagtggt
     3361 ttagccgtca actccttatc ctcggcacag accgcctccg gtaccgttga ctgcaccgta
     3421 taactaccac tccatctcat tctcagaacg ccctatatta cgttctgggg cattctgctg
     3481 actagccaac gttttctctt gatctaatat ttttagctct tcagatattc ccatatcttt
     3541 ttctacaagc ccaaaacgaa ttgataaacg agtgattgat ttttgaaatg cgtttttcag
     3601 ttgagttaac cgccgacgca aagaacgatt ttcttccttc agctttttgt tttcagcaac
     3661 aaccgctgac acatcaacct tttgttgatt tttcaaaaaa gaattttctt gttctaacgc
     3721 catgttttta tcaaataaag cgatattttt atctaatgta tttccaagcg attcattcgc
     3781 catatcagca tattttttgc tgttcgtagc attagacgct ttttgataca aatctacaaa
     3841 atcagcacta gataagcgat aagaaccatc tgataatttt tcaaaactat tgagtggcac
     3901 aggatctttt tgaaatacaa cagccagctc tttttccaaa tcgataatcg attttttctc
     3961 ttcttcaatt ttcgccttct cagaagccac cagagccttc tcacgctcgt ttttttctct
     4021 ttgcttagaa atatacctca tttccgcaga aa
//
LOCUS       DACCCS010000125         3923 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.135, whole genome shotgun sequence.
ACCESSION   DACCCS010000125 DACCCS010000000
VERSION     DACCCS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.135"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(573..1130)
                     /locus_tag="IVK82_002782"
     CDS             complement(573..1130)
                     /locus_tag="IVK82_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393785.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IVK82_002783"
     CDS             complement(1309..1920)
                     /locus_tag="IVK82_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393786.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IVK82_002784"
     CDS             complement(2033..2290)
                     /locus_tag="IVK82_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393787.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="IVK82_002785"
     CDS             complement(2887..3165)
                     /locus_tag="IVK82_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393788.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="IVK82_002786"
     CDS             complement(3199..3450)
                     /locus_tag="IVK82_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393789.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DACCCS010000126         3906 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.136, whole genome shotgun sequence.
ACCESSION   DACCCS010000126 DACCCS010000000
VERSION     DACCCS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.136"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(45..1364)
                     /locus_tag="IVK82_002787"
     CDS             complement(45..1364)
                     /locus_tag="IVK82_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6393790.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="IVK82_002788"
     CDS             complement(1361..2014)
                     /locus_tag="IVK82_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6393791.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="IVK82_002789"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="IVK82_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="IVK82_002790"
     CDS             complement(2724..3440)
                     /locus_tag="IVK82_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP6393792.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3564..3749)
                     /locus_tag="IVK82_002791"
     CDS             complement(3564..3749)
                     /locus_tag="IVK82_002791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393793.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
BASE COUNT         1253 a          737 c          490 g         1426 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcat
//
LOCUS       DACCCS010000127         3883 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.137, whole genome shotgun sequence.
ACCESSION   DACCCS010000127 DACCCS010000000
VERSION     DACCCS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.137"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            82..3123
                     /locus_tag="IVK82_002792"
     CDS             82..3123
                     /locus_tag="IVK82_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP6393794.1"
                     /translation="MIHLVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDC
                     QKLLGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRF
                     KVAYFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKN
                     QYTGQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPF
                     NQGSNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDRE
                     VTKKTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIH
                     DQLNYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGR
                     KIIITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEY
                     YELEGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFE
                     PFHVYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLH
                     PWAVTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDL
                     DVLVAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGF
                     DEPLLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQ
                     TNLREEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKP
                     IMDRYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKE
                     TRENIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEII
                     HKINERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDK
                     VMEQYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
     gene            3313..>3883
                     /locus_tag="IVK82_002793"
     CDS             3313..>3883
                     /locus_tag="IVK82_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAP6393795.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFIS"
BASE COUNT         1329 a          621 c          824 g         1109 t
ORIGIN      
        1 atagctgaat tgatggaact tgagagttat aaaaaagctc tcatctacga atacgtaacc
       61 ggtaaaaaag aagcctagga ggtgattcat ttggtagaga acaaagaaat tcgttttgag
      121 caggatattg agacttcact gactacgtat ggcaaatgga agaaagaaac attcaatgaa
      181 tcaaactttg atcccataat tggtttggat tgtcaaaaat tgctaggctt tgtccagaag
      241 actcaaccaa aacagtgggc acgtcaccaa cgaaactata acaatgatgg agaagtcaag
      301 ttccttcaac gctttaatca agaggtagaa acccacggca tgttacatgt attgcgcaat
      361 gggatcaaag accgcggtgt gcgctttaaa gtagcttact tcaaacctgc ttcagagctg
      421 aatgatgaaa cgatggaaaa gtatgaagca aatagtttta cctgtactcg ccagtttgct
      481 tacagtccta ataaccataa aaccattgat atggttttgt cgttaaatgg tattccgctc
      541 gttgctttag aattgaaaaa ccagtacact ggtcaaacag tagaaaatgc caaactccag
      601 tttcaaaaag atcgtgatcc aaaagaattg gtatttcgtt ttaatcgccg cttcttagtc
      661 tattttgcgg tggaccatac agatgtttat atgacgacaa agttggacgg taaaaaaacg
      721 tacttcttgc cattcaatca aggttccaat gggccgggaa gagttggtgg agcaggcaat
      781 ccgtccaata aagatggcta cccaactgcc tacttgtggg aaaaggtgct tactagagac
      841 aatttgatgg atattatcca acgttttatg aatctagaga ttaaaaaaga aaagcaaggc
      901 gacagagaag tcacaaagaa aacgttgatt tttcctcgtt atcatcagtt agatgtggtt
      961 cgaaaattgg ttgcagatgt aaaaagccat ggtagtggag aaaattattt gatccagcat
     1021 agtgctggtt ctggaaaatc taatagtatt gcatggctgg cataccattt agcctctatt
     1081 catgatcaac tgaactaccc aattttttca tcaattatta ttgtgactga tcgaacagtg
     1141 ttggatcgcc agttgcaaaa tactatcatg aactttgatc atcagacagg tttggtagaa
     1201 acgattgacg atcgcaaatc ctctcaagat ttaaaagaag ccattaatga tgggcgtaag
     1261 atcattatta ccactttgca aaagttccca gtgatctatc aggaagtaga tcaagtggaa
     1321 ggtcgtaatt ttgctgtgat tgtggatgag gcacattctt cacaaactgg gaacagtgcc
     1381 cagaagctaa aagccgctct ttcggacaaa tctaaatcct tggaagagta ttatgaactg
     1441 gaaggcgaag ttgacgatgt attaccagat gctgaagatg aactagttca acaattggta
     1501 gcacaaggta aacatcaaaa tttaagtttc tttgctttta ccgctacacc gaaagaaaag
     1561 acattggaaa tgtttggtac gaaacaacca gatgggcatt tcgaaccctt tcatgtttac
     1621 tcgatgcgcc aggcgattga ggaaggcttt atcttagatg tgctgaagaa ctatatgacc
     1681 tatagtactt cctacaaaat tgcccgtgaa attgaagata acccagaatt accaaagagt
     1741 gaagcaaccc gagcgattgt tcgtttccaa tcattgcatc cttgggcagt tactcaaaag
     1801 acggctgtga tggttgagca attccgagcg gttactcgta cagctattgg tgggcaggca
     1861 aaagcaatgg tcgttaccgc atcacgctta cacgctgttc gctatatgaa agaatttaaa
     1921 aaatatatag cgaacaaagg ttataatgac ttggatgtgt tggttgcttt ttctggttcg
     1981 gtagtagatg gagagactga atatacagaa acgaagatga atgtcacaaa atctggtgag
     2041 aatgttcggg agaatcaatt aaaggaagct ttccatacag ataattttaa tatcttgatt
     2101 gttgctgaaa aatatcaaac gggttttgat gaaccgttgc tgcataccat gtttgtcgat
     2161 aagaagttaa agggtgttaa agctgttcag actttatcac gactcaatcg aacaatgcct
     2221 gggaagaagg atacctttat tcttgatttt gtaaatgatg ttgaagatat tgaagctgct
     2281 ttccaaccct tttatgaaca aacaaatttg agagaagaaa tcaatgttaa cttgatttat
     2341 gatactcaaa gcaagctacg aaaatttaat gtttacaatc aaggtgatat tgaacgtgtc
     2401 atgaaattgg tgaaacaagc ccaaaagcaa cagaatgagc gcttgcttgg acgaatttct
     2461 agcatgttta agccgattat ggatcgctat gaggaaatga acaaggataa tcagtaccag
     2521 tttcgggtga ctctacgtaa ctttggcaag tggtatgatt atatttcaca acttgatcgg
     2581 ttattcgata tggaactatt ggaggaacga atttttactg gatacttgct gaagtttcta
     2641 cctaaagaaa cacgagaaaa tattgatatt tctgacaaag taaagttgga atattataag
     2701 ttacgtcagg acttttccgg tgaaatccat ctagtgaata aagaaggcga gctagtaaat
     2761 ccaaagtcaa ttgatgcagg aatcaaaccg ccggatgaac gagattcact agaagaaatc
     2821 attcataaaa tcaatgaacg atttccaaat gattttgacg aaggagaccg ggtgttagta
     2881 gagattttgt atcggtcatt tgccgataac ccagatttga aggttgtcag catggctaag
     2941 aataacgatg ctcagatgtt tgaaaaatcg ctgttccctg atgtatttaa agataaggtg
     3001 atggagcagt acgagaagaa caatgcagct tttgagaaaa tgtttgggca tgatgataag
     3061 tactttgatc tagtatatac attagttgct agagatttat acaagttatt acggacgaaa
     3121 taaaatttat aaataaggat tagacaatac aatttctctc aaaaataaag gagaagttgt
     3181 attgtttgct tttaagaaag ccctgaaaaa tttgttacgt actaaattgt ctgaactctg
     3241 tcatatcagc tatgtcagaa taaaggaaat agatttattt ttataaacag tgtcgtctcg
     3301 gagatttaaa ctatgtcaac gacaattgaa gtgaacaaac aaagcgtgaa gcagtttctt
     3361 gaaacaggga aaatcaaaaa attcgttatt ccagaatatc aacgtcctta tgcctggact
     3421 gacgagcaaa accaagtact atttgatgat ttagcggagt acacagcaaa caataacgag
     3481 agtacttact ttttaggaag cattgtggct tatgaaaatg atcataacga gcaagaaatc
     3541 attgatggac agcaaaggat aactacgcta tttttatttt tgagagctat ctatgctaaa
     3601 ttagaaaata gttgtgaaaa ggaagcttta ttcttgaaaa gccaaattga acccgcgtta
     3661 tgggaacaag atgatttaac aggtgaggtt aaaccagata aaattctgat tatgtctcga
     3721 gtaatggggg atgaggggaa tgaagaattt gctagtattc tagtaagtgg tgaagcagat
     3781 gttaaatcaa aaagtaatta ttcgaagaac tatattttaa ttcaacatct attaaatgag
     3841 tatgcaacaa acgaaccact atcattttat cgttttatca gta
//
LOCUS       DACCCS010000128         3878 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.138, whole genome shotgun sequence.
ACCESSION   DACCCS010000128 DACCCS010000000
VERSION     DACCCS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.138"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(718..3867)
                     /locus_tag="IVK82_002794"
     CDS             complement(718..3867)
                     /locus_tag="IVK82_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP6393796.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          637 g         1362 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccct
//
LOCUS       DACCCS010000129         3814 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.139, whole genome shotgun sequence.
ACCESSION   DACCCS010000129 DACCCS010000000
VERSION     DACCCS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.139"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            98..2197
                     /locus_tag="IVK82_002795"
     CDS             98..2197
                     /locus_tag="IVK82_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP6393797.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2393..3256
                     /locus_tag="IVK82_002796"
     CDS             2393..3256
                     /locus_tag="IVK82_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP6393798.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3361..>3814
                     /locus_tag="IVK82_002797"
     CDS             3361..>3814
                     /locus_tag="IVK82_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP6393799.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1525 a          636 c          772 g          881 t
ORIGIN      
        1 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
       61 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      121 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      181 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      241 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      301 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      361 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      421 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      481 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      541 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      601 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      661 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      721 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      781 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      841 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      901 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
      961 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1021 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1081 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1141 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1201 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1261 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1321 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1381 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1441 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1501 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1561 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1621 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1681 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1741 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1801 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1861 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1921 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     1981 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2041 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2101 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2161 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2221 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2281 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2341 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2401 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2461 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2521 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2581 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2641 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2701 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2761 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2821 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2881 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     2941 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3001 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3061 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3121 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3181 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3241 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3301 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3361 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3421 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3481 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3541 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3601 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3661 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3721 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3781 catggaatct gtcaggaatg ctatgaaaaa gaaa
//
LOCUS       DACCCS010000130         3662 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.140, whole genome shotgun sequence.
ACCESSION   DACCCS010000130 DACCCS010000000
VERSION     DACCCS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.140"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(679..1719)
                     /locus_tag="IVK82_002798"
     CDS             complement(679..1719)
                     /locus_tag="IVK82_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP6393800.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2068..2394)
                     /locus_tag="IVK82_002799"
     CDS             complement(2068..2394)
                     /locus_tag="IVK82_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393801.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2381..3169)
                     /locus_tag="IVK82_002800"
     CDS             complement(2381..3169)
                     /locus_tag="IVK82_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6393802.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1117 a          606 c          510 g         1429 t
ORIGIN      
        1 ggcactctct tcgggttttc ggtctgtact gaaatcaagg tattattggg aatcccagct
       61 taaatcatag ataccgtaag ggattttatt ctttatttaa aactttgcaa cagaaccgtc
      121 cgttgcgggt aataccgtaa cactgaaggg cacgcattaa ggcgtcatct taatgctttc
      181 ctgtaggaca ctgacggaga ttaccagtct cccgagcctt acaggggcct ttcttttttt
      241 tgtattttca attatcacaa tttatcgtcc aaaagtgcag aacgaaaatc aattcatcac
      301 aagcatatca gacaattaaa tttaaaacaa ctaaaaatat acaaaaaagt gtaattgaat
      361 attagcagca ctgcctgaaa agaagtaata tcaggctttt cagcttgggg aaataagtga
      421 attacctctc ttcatgaatt attataactc aaaaaagcac atatgcaaac actagttccc
      481 tatttttttg aatgaaaaat aactaaaata tctaacgatt atgactttaa aacctacgtt
      541 tgtgattgtt ttcataaact tttaatctaa aaactccgga ataatagcta tttatttgta
      601 acatttgtaa taaaattaaa atcataaaaa agttctaatt taaaattgat catttttata
      661 gtgatcgctt tttatcaact actctagcca attttccgta ctaactgttg gtaattcgtt
      721 aaatgtttct ctttgttcgt ttgcaagctt tgccgcaata gctttttcga caaaccaatt
      781 ttttacataa acgaaaatta gattctgcaa attgtttact ttttcagttt tttgtttttg
      841 ataagctttc agcaaagtca tatataatcc tttgtcagca tcaattccat aagattccag
      901 ttcttcataa acaacaactg tttgctctaa ttcttcagct tttttctttg cattatgaat
      961 agtttggacg gttttttgag cgtcttcgta agtactagaa aatgttttta tcaagtttaa
     1021 tacattttct ggtacaaatg tatttatact gtcctctttc atgatgtcga cgaagttatc
     1081 tagtaaaact tgattttgta actgatcagt ttcagtgtct attatgtgtc tattagtgtc
     1141 tatagaatta gaatagaata gattgtctgc gattttcgca attacggttg agtccttgat
     1201 atgactggat tttgccgtat ttgcgatttt cgcaattacg gtggtatcat tgatttctcg
     1261 cggttttgcc gtatttgcga ttttcgcaat tacggtggta tcagtattgt cttcctcttc
     1321 catgatttta aagacgtctt gattcgttac ttctggcttg cctaagtaca ataaattagg
     1381 catggattca atcttaccgt cacgtttatt tactcttcct ctcttctgtt tcagaagacc
     1441 tgctttttct aattcctttt tgattttaga tactttacct tcgcggcatt gtaacaattt
     1501 cattaattct tcaactgtga atatgaaaaa tatattatca tcagaatcta cccagttatt
     1561 tttgactgaa taatcaaatc gatctttaag tagcatataa gcaatttttg catcatttga
     1621 tagatccatg tatttttcgt tagtaaaaaa taccttaggc atttgataat atttttcacg
     1681 ataaatttct tttgatttgt aataattaaa atcattcata gccaatcctc tttctgtagg
     1741 atagcgtttt gttcaaaact atgttacaat gaattaaaca aatatatcta tccactatat
     1801 atttttctac tgctgataag tcgccaaact ttgttaaagc agtagtcttt ttttatcttt
     1861 ttttagttta ccttaaaaaa taaattaaag caaaccgctc tatttcaagg tttaagctca
     1921 tagtaactat atcacgatat tacaataata ttatatcacg atatcgtaat attacaatat
     1981 gaagataagc gacttatcag ctttgatact actgattttt ataaaaaaaa tatcacgata
     2041 ttatgatatc gtgatattga atgtaaatta aattttattt tgtttctttg ctagtttata
     2101 tccttcaatc aagtattctt caggtatgga ctttaatagc gcatcttcta taacgtctgg
     2161 tttacttttg tctgattcag caatagtcgt ccaatatttg agtgctgaca ttagaattgg
     2221 atttatacga atgctatctg ctgttcttac actacttcct tgatctttta tgactttact
     2281 tatatttttc ttggttaatt cgatttcttc tacttctttt gatttaaaat tctttttatt
     2341 tttatttcct agtaatctac ttactgcagc ttgactgcga ttatcttctg ccatcaaaat
     2401 caccaacttc ttttaaatat tcttctgtaa atgctttata gtcatcttca gctgttgagg
     2461 gtttattaaa attgattaga tctgatccag cagcttgcgc ttcgtcaacg gttattgtcc
     2521 tacgaatata agttttgaaa atttttgcac caatgactct agaaatttcg gttgctgcag
     2581 acgtcatttc tttagaaatg ttataacgct tattatactt agtaaataga actcctcgta
     2641 cttcaagctt gtcgttcgta tattctttta ccgtttcaat agtttcactt aactttatta
     2701 gtccttttaa tgagaaatac gcagcttcaa ctggaatgat aatttcatca gaaacagtaa
     2761 atgcgttgac tgtaagcatt cctaaacttg gtggacaatc aattataata taatcatagt
     2821 tgtccttgat tggttgaagt actcttttaa gcctgtgttc acttcctact tgagtaaatt
     2881 ccctctctgc agaagaaagg gctatgtcgg ctggcaaaac atcaattcct ttatagtcca
     2941 gaatagcatc tgtaatgtca gcctcccctt ttaatacttc ataaattgta ggttggtttg
     3001 ttccatctat atcgaaacct acattatctg tagcatttcc ctgaggatca atgtccacta
     3061 aaagtacatt tttaccgtaa acaaatttca aacatgctgc taaattaacg ctagtcgtag
     3121 ttttagaaac tccacctttt tgattggcta ctgacaatat tcttgccatt aaaatatccc
     3181 ccattcatct tatattataa tataattata tcataatatt gtgatattgc aatatcgtat
     3241 atgcattata ttgtaatatt gtgtgattat tttgttttaa agatatatat cggttcggat
     3301 ataaagatta ttcatatgtt attcgaaatt aaaataatca ctttttatta ctttaaaatt
     3361 ttttattaat gataagaaat atgttttgcg agcagcaact agaataattt aaacattact
     3421 aaactgtcta tattgttact aataattccc ttcttatagt gcattttctt agaaaaaaac
     3481 aatattacga tatcatgata ttaagatatc gtaatattat acaattgtga ttgatattat
     3541 atatgttttt ttattttttt atcatgttta atcttgaagg aatcatattc tttttctaaa
     3601 gacaggagct tttcataaac atcagacatg tttaaactag aggccaggct taaatcataa
     3661 ac
//
LOCUS       DACCCS010000131         3641 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.141, whole genome shotgun sequence.
ACCESSION   DACCCS010000131 DACCCS010000000
VERSION     DACCCS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.141"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            138..995
                     /locus_tag="IVK82_002801"
     CDS             138..995
                     /locus_tag="IVK82_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6393803.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            996..1544
                     /locus_tag="IVK82_002802"
     CDS             996..1544
                     /locus_tag="IVK82_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393804.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            1804..2532
                     /locus_tag="IVK82_002803"
     CDS             1804..2532
                     /locus_tag="IVK82_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6393805.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWCKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLGYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            2562..>3641
                     /locus_tag="IVK82_002804"
     CDS             2562..>3641
                     /locus_tag="IVK82_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HAP6393806.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEKAIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYRKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAIDTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIE"
BASE COUNT         1312 a          497 c          767 g         1065 t
ORIGIN      
        1 cttttcctta tttatgtact accttttcct tattaattat aaggtagtac atatatatct
       61 tttttttggt agtaactaaa aataaatatg ttataatagt atcaaatgaa tattgctaaa
      121 agaaaagagg taggaacgtg gtagcgaaga aatatgtaat cggtaatttt aaaggtggcg
      181 taggtaaaag tacgtgcgcg caaatgttag ggtttgaatc agcagttaat gaaggaaaaa
      241 gaaccttgat aattgacttg gatatgcaag gaaatacttc tgacgtgatg aacttaacgc
      301 atatgaactt cactaacgaa gaagggggcg gagaaggtga accgattgtt tacgaaaata
      361 ctgtgactga cgtgttgata aacaatcttc cacctaaaga ggctgtctat aaagtaatca
      421 ataacctcta tatacttcca gcagacatgt cctttgaaat gtatgatgat tggatcaaag
      481 accaatataa tgattcatta tcccaattca aatatatgaa agaaaagcta gaaccattat
      541 ttgatgagtt tgacgtcatc tatttagatg tcccaccttc tatttctgta tatagtaaat
      601 ccgcgatgta cattgcggac tgggcaatcg tcgtcttaca gacccaagta aaatcgatga
      661 gaaatgcgat gcaatatctt gagtatatgg acttttttgt gaaagaattt gatacaaatt
      721 tatatgttgc tggtgttatt cctttcatgt tggaaagcgg tgacgcagta gatcaggaaa
      781 tgtacaaaca agcccaagag atttatgggg aacatttgat taaaaatgtt gtactcaaaa
      841 atgcacgctt aaaacgatac gacggatcag gtattacgat ggagaagaca aagaaaggtc
      901 tcttaaaaca atgggatcgc aaaagccacg aattatttat cagtattttg aaagaattgg
      961 aagaacacga gaattggtat gcggaggtgg agtaattggc tgacatcaaa ggacttgata
     1021 tttttgggaa caacaaagga ctcgccaaaa taaaaaaaga agcagaagca aagaaaaagc
     1081 agcaagcaat tacaccatct gaaaataagc aattagaagt gcaccaagtt caagaaataa
     1141 aaacttcctc caatgttgaa agacagagta gaggaagaac aaaaaaagtt ggcgcaccaa
     1201 tcagaaagtt tgataaggtt tattccacca agcatccttt aaaactgtcg actttgttaa
     1261 atgcgacttc cagagtttta gtagaaaagt atgagactaa tatgacaaga gatgaattgc
     1321 ttagaaaagc tttagatgag tatattaaac agaatcttac gcaagaagat aagcaagatt
     1381 tgtttaatga cgtggtgaaa gaactagact tgttcagaga aaaatatcca attgttccag
     1441 aattagatga tcgtggagaa ataataagaa ctaccagtca gataaaacaa gataccgaag
     1501 actcactgcg caaagggtgg ggaatgaata ataaagttag ataataatca aagaagaact
     1561 aagtaactac ctttaaacgg tagttactta gttcttcttt gatttgcgtg aaatagaggg
     1621 gatttatttt tctggttacg tgattaaaaa atccaaaaaa tgagcatagg aacggttatg
     1681 attttagggt gtgttttaat gagatattgt gatttcatta acaagtgtat gataatctgt
     1741 tgttgagtta gtaaataagt aatagagcta actctaaaat aaaactatta ggaggagatg
     1801 taggtgaaaa agtttaaaaa gaaaatatat ttaggattgg ttgttggggt gagtatgttt
     1861 gggttcatgg ctcatactaa acaagtaact gcagatacta accaaaaaga aaggattatg
     1921 agccaattag atgactctca aatagagtat gtacccaatg agctaattgt cagtttaaag
     1981 gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg tgtaaaagtt
     2041 atggtggaaa aagatttgac tcttaggaca aatgaaagcg attataataa gcgagatcaa
     2101 gtattattag tgaaattgaa cgaagaaatt agtgtggaag aggcagtaag aacatttaat
     2161 gcttctgata tggtcaatta tgcagagcca aactatgtgg ttgaactaga gccgttgaat
     2221 gatgttgagg agccaaatac aactgattct ttaggaaatc gatcaattat tcttaacgat
     2281 cctaattggt gtaaacaagc taatttaaga gagattagca tgccatccgc ttgggaagaa
     2341 accaagggtt ctaaggatgt gaaggtagcg gtcattgatt caggaattga ttatcaacat
     2401 gaagatcttt ctggaaatgt agatgaaagt ttgggatacg atttttttga cgatgataca
     2461 gatcctatgg atactatggg acatggacac atattgccgg gattattggt gctcaaacaa
     2521 acaacgaatt aggtgttgct ggaataaatt gggatgttac gatggtacct atacgtgtat
     2581 ttggtagtag tggaggtacc agtttggcaa tcttaactga agctattaat tatgcatccc
     2641 aaaaagggtt tccaattatc aattttagta taggtagtgt tgtttatagc agtacagaaa
     2701 aagcaattaa aaactatcct ggattattcg tagcagcagc tggaaataat gggatagata
     2761 tgactgataa acctttttac ccagcttctt tggatatacc taatattatt actgtaggaa
     2821 attctagtgg ttatgaacga agttctgatt caaattatag gaaaacaggt gttgatttat
     2881 tcgctagagg gtttcgtgtt tttagtacat ttctaggaag taaatacacg tatatgtcag
     2941 gtacctcgat ggcaacacct catgtcactg gtagtgctgc attagcgttg tcactagatc
     3001 ctgatctatc tactgatgaa ttaaaaaaaa tcattttaaa tagtgttgat tcattttatg
     3061 aactatctga tttatgtctt acagggggaa gactaaatac gaataatgtc gtacgacaag
     3121 taacgggtaa acttactgca tctaatatca cattaagatt aaatagtgaa tggtcagatg
     3181 aaattgctaa agagatgtgt cacgttcact ggattgacca gtcaggaaat gatttgacgg
     3241 atcaagtggt tgttctaaaa aacgaagtgg atacatctaa ctttggcgat tacgaagtag
     3301 tttttgcatc acctgattta acagatcaaa tctcagttac agtgaccgta cctcgattac
     3361 gtaaacttac gctaggaaaa gaaacagcaa ccattgatat tggttccgca tgggatagtg
     3421 tcatagccat tgatactttt caagtgaaag ctatagatga ttttgggaat gatgttacaa
     3481 atcaagtaca ggttactatt aatgctgata tagatacaat taattcaatt caagcagtga
     3541 ttaagtttga agttccagaa ttaggcttag cccaaattgg tagactgaat attactgcaa
     3601 aaaatagtac aatcaatgga ttgaatgaag ttagcatcga g
//
LOCUS       DACCCS010000132         3569 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.142, whole genome shotgun sequence.
ACCESSION   DACCCS010000132 DACCCS010000000
VERSION     DACCCS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.142"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..426)
                     /locus_tag="IVK82_002805"
     CDS             complement(<1..426)
                     /locus_tag="IVK82_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP6393807.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLE"
     gene            complement(566..1198)
                     /locus_tag="IVK82_002806"
     CDS             complement(566..1198)
                     /locus_tag="IVK82_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6393808.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1205..2272)
                     /locus_tag="IVK82_002807"
     CDS             complement(1205..2272)
                     /locus_tag="IVK82_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6393809.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2269..3000)
                     /locus_tag="IVK82_002808"
     CDS             complement(2269..3000)
                     /locus_tag="IVK82_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP6393810.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3168..>3569)
                     /gene="greA"
                     /locus_tag="IVK82_002809"
     CDS             complement(3168..>3569)
                     /gene="greA"
                     /locus_tag="IVK82_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP6393811.1"
                     /translation="KRGEIIERIKIARGFGDLSENSEYESAKDEQAFVEGRITTIENM
                     IRFAQIIDNDGVDADEVSIGKTVTFIELPDGDEEEYTIVGSAEADPFSGKISNDSPIA
                     QALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
BASE COUNT         1006 a          798 c          556 g         1209 t
ORIGIN      
        1 ctctaagtag tctagaatat tctttgatac taagttatgt gcaatccgta tccccatgtc
       61 tcgctcaggg tgtacgacac gatctgctcc gatcttctct agtacacggg catggtactc
      121 attttgggct tttgctaaaa tattcggtac gcccatttcc tttaccatca gagtaactaa
      181 gatactggct tggatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatacc
      241 taatgaacga agtgtcatct catcctgtgc atttgccaca acagcatgag tggctatatt
      301 catatattca ttgactcgat cttcattact gtcaatagcc agtacttctt gtccagcttc
      361 gatcagtgta cggcagatgc ttccgccaaa tcgtccaagg cctacaattg caaaattttg
      421 tttcaccatt ttcctccttt agtatcttct tttctcagta taacaaagcc tttccaaaaa
      481 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
      541 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttaaac gcatagatag
      601 ctgcttgagt tcggtcttct acctctaact tagctaatat attcgacaca tgcgttttaa
      661 ctgttttcag tgtgataaac aattcatccg cgatttcctg attacttttc ccttcagaaa
      721 tcagcatcaa aatttcattt tctcgattcg ttaattcttc atgcaatact ggatcatttc
      781 gccgactcat tttttccatc atttttgttg tcacttcagg ttccaatact cgctcgccgc
      841 gttgcgtagc ccttatagca tcagctattt catgtgcggt agacgtcttc aacaaatatc
      901 ctgctgctcc tgcttcaatt gcaggataaa ctttttcatc atcaataaaa cttgtgacga
      961 taatgatttt ggcttttgga caatctttta agattgcttt tgttgattcg atgccatcca
     1021 tttcttccat gactagatcc attaaaatga catctggtcg aagtgccatc gccttttcat
     1081 agccttgacg tccattttca gcttctccaa tcacttcaat gtcttcttgg atagataaat
     1141 aagaagagac gcctaagcgt accatttcat ggtcatctac taataaaact tttatcatct
     1201 ttatctatgc ctccttcatc aaagggacct tgatctccac gctcgtacct tgccctttaa
     1261 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
     1321 cataacttcc cgttttggtt tcattcatat taaatcctgt cccgtcgtca atgatacgta
     1381 ataaaagatt gttatctatt ttatgcaaat agacttctaa ttcattagcc ttagcatgtc
     1441 ttaatgtgtt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
     1501 ttaattttac atcttctaca tcccatttta gcgaaatctg gattttattc tgcaattcct
     1561 tcaataactg ctcaatgcct tgttttaaac ttttttcttc taaattgact ggacgcagat
     1621 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgcc accattttca
     1681 actgcttacg gaacatttct ggtgtctcac ttttttctgc ttgctccgtc aatgccgaca
     1741 tcatcatcat cgctgcaaat aattgctgtg aaactgaatc atgaagctca cgagctaatc
     1801 gatgcctttc taattctaaa atctcttctt tggtttgtcc atctacatat tgaggacggc
     1861 tagtcataat ctgtaattca ctggatactt cgatcatttt ttcttttatt ttagcaattt
     1921 ccatatcaat atctttgatg tacagatcat ccgacgcgct tgggatcggc tgatgcaaaa
     1981 gtttggattc ataattgcca gaagaaagtg cacgaagctt ttcttcgatt ttcccgtatt
     2041 ttgccttttg aataagtgat aacaatagaa atacgatcaa tcctacacct aatgaaattg
     2101 ccatcaaatg gaagataagc ggaacgtata ataaacgcgc acgcagcaat tctccccacc
     2161 aatgactttg gttactggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
     2221 ccgcaatccc cgaataaaca gctagcattg ctttggatat tcttcccatc atattcgtat
     2281 cacctcgaca tctccaacca aggtattggt aatgatcttc aatctgcgag gattttcatc
     2341 ataatcactg ctgcagattt tgatctgttc gttttttaag gaaaactgct cttcttcaaa
     2401 caagacattt cctactaatg tcgcatgctc taaacgaata gccactccca ggggtactaa
     2461 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
     2521 tgtattaccc aaatcgatga tagtatcacc agaaatgatg gcaatgttga tatcatccca
     2581 ttcatagacg tcgtttccaa tacgttggtt accaaataat tgctgtcttt tacgctcatt
     2641 attatgtgtt tttaattgct ctgtttggac catcatgatc tgtttttttc gccaaaaagc
     2701 attttttgtc aaatctattc ctgaaatctc tacacctttc agcccgataa ataaaacggc
     2761 caatacaacc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaagct
     2821 ccctaagatc aattgaaaat tttgaaattt agtacgagga tatttgcgca tagctagata
     2881 tatattgaaa ataccgaata ttactaaaag caaaagtcca gtattgttta cgatttgcca
     2941 aacagcaaat aacagcagca gtgcttcgac taccacaaaa aaacgccaag aactattcat
     3001 cgctttttct ccctcctgac acattctcat ttcatcatac ctattttttt gactgttggc
     3061 tataggactt tagatgtaag atcgttcagt gaaacctgca atactcaaag agaaaagatc
     3121 tagagtgcgt catcagtaag atgaactctc tagatctttc agtcagacta cgcttgttct
     3181 actttgacga ttttaactaa catatcgcca cctggtgtag caatggttac ttcatcattt
     3241 ttacgtttgc caatcaatgc ttgcgcgata ggagaatcat tggaaatctt tccagaaaac
     3301 gggtctgctt cagcacttcc gacaatcgtg tactcttctt catctccatc aggcaactcg
     3361 atgaacgtaa ctgttttacc gatcgatacc tcatctgcat ctacgccgtc attatcgatg
     3421 atttgtgcaa aacggatcat attttcaatt gttgtgattc ttccttcaac aaatgcttgt
     3481 tcatctttag ctgattcgta ttcggagttt tcggaaagat ctccgaaacc gcgagcgatt
     3541 ttgattcgtt caatgatttc tccgcgttt
//
LOCUS       DACCCS010000133         3533 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.143, whole genome shotgun sequence.
ACCESSION   DACCCS010000133 DACCCS010000000
VERSION     DACCCS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.143"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            167..307
                     /locus_tag="IVK82_002810"
     CDS             167..307
                     /locus_tag="IVK82_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP6393812.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            407..796
                     /locus_tag="IVK82_002811"
     CDS             407..796
                     /locus_tag="IVK82_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP6393813.1"
                     /translation="MTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTGLAKAWKLKEI
                     SSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEGISFLENISVF
                     LPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            793..1803
                     /locus_tag="IVK82_002812"
     CDS             793..1803
                     /locus_tag="IVK82_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6393814.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     SDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPD
                     VNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIP
                     KR"
     gene            1918..3126
                     /locus_tag="IVK82_002813"
     CDS             1918..3126
                     /locus_tag="IVK82_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393815.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
BASE COUNT         1293 a          507 c          668 g         1065 t
ORIGIN      
        1 agtggcacag cggttgacga tattgaagat gtcgatcagt tgaagcaatt aaatgctcta
       61 ctgacaaaac aattggcgat atcggtacag gaacaagaaa aaatgcaaca aatgttagct
      121 caattgacga tggaagtcgc aagtattaag aatggaggga aaagtaatga atagttttcc
      181 gggctttgaa aatataaaac aattttatga ttggggatgt tatacggacc aagatttact
      241 tgactatgta aacatgaact gtttaacaaa agaccaatat aagcagatta ctggaaatga
      301 aatttaatta agcacaaagt taaataaaaa gcgtactcaa acgagtgcgc ttctttgcat
      361 aaaggagaaa tagatattgg aaaaatattt aaatacactc tcagtaatga caggggtgat
      421 aggcgggaca attgttggtt tattgggagg gatggacaat atactacatg ttttaatttt
      481 tcttgtgggt gtggacttct taactggact tgctaaggca tggaaattga aagaaataag
      541 tagtgagata ggtttcgaag gattgttgaa gaaagtctta atcttcgttg tgattgccgt
      601 tgcggttgac gtacagaaaa tcgttgggaa ttctatacct ctaagagaaa tagttattat
      661 gttctatgtt gcaaatgaag ggattagttt tttagaaaac atttcggtat tccttccttt
      721 gccagataag ttaaaagagg tattccaaca aatacgaaat gatactgaaa ataaagaccg
      781 aggaggaacc aaatgaaaaa gaaaatcttt gtaggagcta ttatagctct ttttttattg
      841 ccaataaacg cctttgctta ctcgatcaac aatgaattca atttgggcgc aaacgaaggt
      901 agttctcaag tagcaaataa ccaatacatc ctattgcatg aaacagcaaa cgaaactgcg
      961 acaggacgaa atgaagcgca gtacatgaaa cgttcttggt acaatgccta tacagcgtat
     1021 attgttggcg atggtgggat cgtttaccaa gttggacaac ctggttatgt acaatatggc
     1081 gctggttcat atgctaatgc aaatagtcct gtgcaaatcg aactgcaaca tacgcacgat
     1141 aaagtaacgt ttgaaaagaa ctacaaggca tatgttgaat tagcgagaga ttccgctaat
     1201 aaatacggta ttccacttac attagacaca ccgtataatc aaccaggaat caaatcacat
     1261 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
     1321 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
     1381 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
     1441 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
     1501 aacttacacg tcgctggatg gcacatcgct aactataaat acgagtatat tttcattatg
     1561 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
     1621 gtaaatcaag cttataatac ttctggaaac gttggctatc atgtatcttt taacatgcgc
     1681 aatttcccta ataagaaagt atacgtcatg atgcgggcaa cgaatgatcc agaaggaaac
     1741 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
     1801 taaaaatagc tcctcgttga ggagcagtac atagaattga aaattaacgt taaatcaaaa
     1861 aaatatttac aagatgttta tttaagtaga taataaaaga aaaaagcagg tgaaattgtg
     1921 acagcagaaa ttggtattat gaataaaagt ggaatcgtgt tagcttcaga ttcagcatct
     1981 acgattggag atagcaaagt atataatact gcaaaaaaat tatttacttt ggattccatg
     2041 cattctgtag gtatcatgat atatggtaat gctgaattta atggtattcc ttgggaaata
     2101 atcattactc aatataagaa aagcattggt agttctgttt ttaatacttt ggaagaatac
     2161 gcggataatt ttatagaatt tgtcaaaaca gcatctttta ttagaagtga acagaccgag
     2221 caagaacaaa tgattggggt ttttcaaaaa ataatttcag gtttatttga gagtatagaa
     2281 gtagatatta actttttaat aagccaaggt acacaaatag acaaagatgt cctagtgaag
     2341 ttattacaaa caaaaatgaa taccaatttg tctcaacagt cccagacatt cattttagat
     2401 attgaaaagg cgttatttct tagtaactat ggagaaatat taaaagatat tcttaatagt
     2461 atttctacaa tggaaggtgt ttcagaagct atatcagagg aaattcaaag ttacgtttat
     2521 gaaattataa ttagagacga tgtttattct tcccctacag gaatagttat agcaggatat
     2581 ggaagaatgg atattttccc taaactatat tcttataata tgtttggatt tgttatgaat
     2641 attctgaaat actcagaata tgaaagtgct caaataggaa atgacaatgg ttcattaaga
     2701 tctactatat taccatttgc tcagtctgat gtggtaaata ctgtagttca aggggtagat
     2761 ccacaaatta ctaattatct gtcgagtcaa gtcgatagtt ttgacgataa cggaaaaaat
     2821 acctatatta atattataaa gaatatttca gagtttcaac aaaatcaatt catatttccg
     2881 ttactaaaca tgattgcttt actcccagtt gaagaaacag caattatcgc agagacttta
     2941 ttaaatttaa ccagttttaa acgaaaatat actacttctg tagaaacagt aggtggccca
     3001 attgatgttt tagctattac acctaatgat ggccctattt ggattaaaag aaaacattat
     3061 tttgatattg ataataatat tggatacaga ttgcgaaagg agagagcgaa tgattacaat
     3121 aattaaacct caggaaaaat cccctgtttt gaaatttaat gagagcaatc tagcccataa
     3181 taaaatgagt ataactgata cagtatttga tgtaatggtt gagcaacaaa aaaaaattaa
     3241 taataacaat agacaaagaa aagaaattaa ccaatattaa tgttttttct gttgcgtttt
     3301 ttgcagtcat aatttaaaac ctcgctcaat cttgggcgag gtcttttttt atttttctga
     3361 aaagttaaag taaataatag cataaaataa tatattttac aaagaataag tacaatctag
     3421 ttttttgcta ttaaatgtgt aataataaat atgccatcac aacatgaaga atgaaatcca
     3481 ttattatcta gtctagtcca ttctttttgt ttgcagtatt tgtgatggct ttc
//
LOCUS       DACCCS010000134         3437 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.144, whole genome shotgun sequence.
ACCESSION   DACCCS010000134 DACCCS010000000
VERSION     DACCCS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.144"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(72..434)
                     /locus_tag="IVK82_002814"
     CDS             complement(72..434)
                     /locus_tag="IVK82_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393816.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(438..746)
                     /locus_tag="IVK82_002815"
     CDS             complement(438..746)
                     /locus_tag="IVK82_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393817.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(755..1174)
                     /locus_tag="IVK82_002816"
     CDS             complement(755..1174)
                     /locus_tag="IVK82_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393818.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(1167..1400)
                     /locus_tag="IVK82_002817"
     CDS             complement(1167..1400)
                     /locus_tag="IVK82_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393819.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
     gene            complement(1403..2371)
                     /locus_tag="IVK82_002818"
     CDS             complement(1403..2371)
                     /locus_tag="IVK82_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP6393820.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(2818..>3437)
                     /locus_tag="IVK82_002819"
     CDS             complement(2818..>3437)
                     /locus_tag="IVK82_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP6393821.1"
                     /translation="SDELRYISRQREENERDKALMASERAKIEEEKKNMLNVKKEMAV
                     IFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQRATRATNAKRNADNMSEQYLQMLEQN
                     TELNEYVATLEEERHLSGRKQLADLQVENKKLKEENRSLRQRLERLKNAFQKSITRLS
                     IRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQRKLERSEDEIEW"
BASE COUNT          983 a          787 c          481 g         1186 t
ORIGIN      
        1 aaaaggagcc gaaagcgtgt cacgctctca actcctttta cgctttattt tgcttcacgc
       61 aaaataatcg cttatcgtaa attcacgtca ttaaatattg acgtaaattt actctcactc
      121 atctgacttt tcttattcgg tatcactccg aataagccgt cagattcatc gggataatct
      181 cgttcgcttg gcgctgattt ttcatcagca agccactcat tatccactga tttttcgtgt
      241 tgctgatcgt cagaaatcga cgatagccac cgctcaaaat cagcaaaagt ttcaaaccca
      301 aaacgcttgc gaagagcctt gcccattgca gcattttctt tctcaatcaa gcgttttttc
      361 tgttggcgaa gtttcgcttg ttcttttttt aatttttctt cttgcgcttt aatatctgct
      421 aaagtcattc ccataaacta catctccttt tctttggaat taataacgga aatttggttt
      481 aactgcataa catgactcat taatttcaac caaaattctt tttctgattc atcagttgtt
      541 tgcgctaaat gcttatgaac aaattcatta gtcaaaactg aagattttgc tagatcagca
      601 ttgataaccg agttgagaac atccatcatt cgttcaagca tttcactata attatcttca
      661 tccggcaaat tcaaataatc agctaaccgt tcatacatca attcaattct atcgactgtt
      721 tcttctctca aatcaatttt atccatgtca cacctcactt ccagaattgc caccattttc
      781 gattttttaa ttcttgattt tctagtaaca actgatcgtt tttttgttgc tgaagcaaag
      841 tcaatttttg ttggtcacga atcatttccg ttaattcaga aatatgccta tctttttccg
      901 ctaattgttc acgcaaaaat tcattttctt tttctaacgg gaaatcattg cctgcatcat
      961 tgaccggtaa atcaccagtc aattgaccac cttttgaccg ataaattgac cgcacaacac
     1021 caatcaattc cttgtctaac atgataaccc cattatcttt ataccgagca aggtcataac
     1081 ctgcattgac cagttgattg cgcactgtat ttttagatat acccaactca tcagcaaggt
     1141 tttttatggt cattttttct ggcaaatcag tcattatctt ctccgttact ttcagctacc
     1201 ccataaacat atttaacagc atgcttaggg ataatgattg gggaatcaat tttattattt
     1261 ttcaaaatca actcatactg cgttacatca agtaacttac cggacatttt tttgctatcc
     1321 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
     1381 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
     1441 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
     1501 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
     1561 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
     1621 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
     1681 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
     1741 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
     1801 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
     1861 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
     1921 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
     1981 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
     2041 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
     2101 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
     2161 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
     2221 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
     2281 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     2341 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     2401 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     2461 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     2521 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     2581 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     2641 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     2701 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     2761 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     2821 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     2881 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     2941 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     3001 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     3061 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     3121 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     3181 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     3241 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     3301 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     3361 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tagaaatata
     3421 cctcaactca tcagaaa
//
LOCUS       DACCCS010000135         3436 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.145, whole genome shotgun sequence.
ACCESSION   DACCCS010000135 DACCCS010000000
VERSION     DACCCS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.145"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            210..1982
                     /locus_tag="IVK82_002820"
     CDS             210..1982
                     /locus_tag="IVK82_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP6393822.1"
                     /translation="MNNNETKKTYELRITADEIKSTDPTFTMKNLSLGDNYQPKDAYF
                     RTAEGEITRRNYGKNERKISNQTLPRLAIQMFEQSIVSLPESERATFPVCRYHSGDEI
                     IRGIFPDVDSFKKKRKSIEFLIYKYNEDKQIVIYCWSIFSTLLFVQECLKRFGTSGDE
                     FILVYKGKEKKEIRVGETQTMTNHLLKSYVLHLLKSKNLILRGAPGTGKTFLANEMAA
                     DVVSNGRTTQINELSEDEKSRIGFVQFHPSYDYTDFVEGLRPTTSDDGVVSFTLKDGS
                     FKSFVEKAKKTKKVNGQDNFEEAWAKFFDAVTEASIEGEGYNKLKTLTGKPVRNLLAY
                     ERNEIQGVYPAKTNTYLNHDQIYNVYRGLPGTPKGGFDAYRKAIVKHLKEEFGLKNYT
                     ASTVDSTNNQNYVFIIDEINRGEISKIFGELFFSIDPGYRGHKEGVYTQYANLHENPE
                     EKFYIPNNVYIIGTMNDIDRSVDTFDFAMRRRFTFLEIKAEDSAQNMLTQEKTKELMT
                     RLNEAIVAKEKGGLSTDYQIGASYFLSIDNGEETMEQLWDTKLYPLLKDYFHGEHKAK
                     EKLEILEKVYSNDGDLDEAETSTR"
     gene            1957..3249
                     /locus_tag="IVK82_002821"
     CDS             1957..3249
                     /locus_tag="IVK82_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine 5'-monophosphate oxidoreductase"
                     /protein_id="HAP6393823.1"
                     /translation="MKLKPLHDNQEINDSVVQHPIITNLVNRDLSSLEKENFIIFPQQ
                     LNQSKDLSEDNLIFQSRNGKIWTCNVVGILSDSTDELRIHSRFANQMKHEDFFLRYML
                     QRVLNYNVINDHFSASKKMTYYDLLVFLFPYYLNEAMRKGTYKEYVKREYNNANIKGA
                     VDVAKHIRSNVPFVGKVAYRTREFSYNNHLTQLIRHTIEKIQNEYDFLLSGDEDTKEN
                     VVLIKQTTPGYARLNQFNILQENILHPVKHSYYEEYSALQQICIQILSEEKSGFGSDK
                     NQIHGIIIDVSWLWEEYIGKVTGWKHYGRDKGLATMHLFQEPNRSPRYPDFTFNNIPI
                     DTKYKKHLDTRNDYNQLVTYIHIMNLDQADTMKGGFLQPTSDPLPANHGYKKIGILSG
                     LGGEIFTYHFFIPQAASSYLEFVEQMREVEAALQTTFQ"
BASE COUNT         1286 a          527 c          630 g          993 t
ORIGIN      
        1 aagcgaagcc atttaaatac ccacaagaaa tgaagcgagc ctggcgtaaa atgttagatg
       61 atgagttgat tccacttgag aatgaattga ggtaaacgag aaaattgaag aaattgtatg
      121 ataaacatgt taataaatga tgctcgaaat aaaatttggt tttattttta cattgatacg
      181 gtaaataaaa tttgaaagta gaagaaaata tgaacaacaa tgaaacaaaa aaaacatatg
      241 aactaagaat aactgctgat gagattaaat caacagaccc aacatttact atgaagaatc
      301 taagcttagg ggataattat caaccaaaag atgcttactt ccgtacggca gaaggcgaaa
      361 taactagaag aaactacggt aaaaatgaac ggaaaatttc aaatcaaaca ttaccgcgat
      421 tagctattca aatgtttgaa caatcgattg tttctctacc agaaagcgaa agagcaacat
      481 ttcctgtgtg tcgatatcac tcaggagatg agataatacg agggattttt ccagatgttg
      541 acagtttcaa aaaaaaaagg aagtccattg aatttttgat ttataaatat aatgaagaca
      601 agcagatagt catctattgt tggagtattt tttcaacttt attatttgta caagaatgtt
      661 tgaaacgttt tggaacatct ggggatgaat ttattcttgt ttataaaggg aaagagaaaa
      721 aggaaatacg agtaggagag actcagacta tgaccaatca tttgctgaaa tcatatgttt
      781 tacatttact caaatctaaa aaccttattc tacgtggcgc tcctggaaca gggaaaacat
      841 tccttgcaaa tgaaatggca gctgatgtcg tttcaaatgg cagaactaca caaatcaatg
      901 aattgagcga agatgaaaag agccgaattg gatttgtcca gtttcatcct agctatgatt
      961 atactgattt tgtcgaaggt ttacgtccaa ctacatccga tgatggcgtt gttagtttca
     1021 ccttgaaaga tggaagcttt aagagctttg ttgaaaaagc aaagaaaaca aagaaagtaa
     1081 atggacaaga taattttgaa gaagcttggg ctaaattttt cgatgcggta acggaagcta
     1141 gtatagaggg agaaggctac aataaactta aaacattgac aggtaaacca gtacgcaatc
     1201 ttctagccta tgaaagaaat gaaattcaag gtgtttatcc tgctaaaacc aatacttatc
     1261 taaaccatga ccaaatttat aatgtctatc gtggattgcc aggtacgcca aaaggtggat
     1321 ttgatgctta tcgaaaagcg attgtaaaac atttaaaaga agaatttggc ttaaaaaact
     1381 acacggcgtc aacggtagat tctacgaata accaaaacta tgtgtttatc attgatgaaa
     1441 ttaatcgggg cgaaatttcc aaaatttttg gcgaattatt tttctcaatt gatccaggat
     1501 atcgtgggca taaagaaggt gtttacactc agtatgcaaa cctccatgaa aatccagaag
     1561 aaaaattcta tattcctaat aatgtttata ttattgggac gatgaacgac attgaccgtt
     1621 ctgttgacac ttttgatttt gctatgcgcc ggcgatttac tttcttagaa ataaaagcag
     1681 aagattcagc acaaaatatg ctgactcaag agaaaacaaa agaacttatg acgcgtttaa
     1741 atgaggcgat cgttgccaaa gaaaaaggtg gattgtcaac tgattaccaa attggtgcta
     1801 gttacttttt aagtatcgat aatggtgagg agacaatgga acaattatgg gatactaagt
     1861 tatatccatt attgaaagac tattttcatg gtgaacataa ggcgaaagaa aaattagaaa
     1921 tactagaaaa ggtatactct aatgacggtg atttagatga agctgaaacc tctacacgat
     1981 aatcaagaaa tcaatgactc agttgtgcaa catccaatca ttacgaactt agttaatcgt
     2041 gacttaagta gtttagagaa agaaaatttt attatctttc cgcaacagtt aaatcaatcc
     2101 aaggatctaa gtgaagataa cttgatattt caatcaagaa atggaaaaat ttggacttgt
     2161 aatgtcgtag gtatccttag cgattcaaca gatgaattaa gaattcattc tagattcgct
     2221 aatcaaatga agcatgaaga tttcttttta cgttatatgc ttcagcgtgt tttgaattac
     2281 aacgtgatta atgatcattt tagtgcatct aaaaagatga catactacga tttgttggta
     2341 tttctctttc cttattatct gaatgaagca atgcgtaaag ggacatacaa agaatatgtc
     2401 aaacgagaat acaataatgc caatatcaag ggagctgttg atgttgcaaa acacatccgt
     2461 tcgaatgttc ctttcgttgg aaaagttgct tatcgaacac gagagtttag ctataataat
     2521 cacctaaccc agcttattcg tcatactatt gaaaaaatcc aaaatgagta cgatttctta
     2581 cttagtggag atgaggatac gaaagaaaac gtggttctaa tcaaacaaac aacacctggt
     2641 tatgctagat taaatcaatt taatatcctt caagagaata tccttcatcc agtcaaacat
     2701 agttattacg aggagtattc ggctcttcaa caaatctgta tccaaatttt atcggaagaa
     2761 aaatcaggat ttggaagtga taaaaatcaa atacatggaa tcattatcga tgtgtcttgg
     2821 ctatgggaag aatacattgg aaaagtcact ggttggaaac attatggcag agataaaggg
     2881 ttagcaacaa tgcatttatt tcaagagccc aaccggtccc cacgatatcc agattttaca
     2941 tttaataata taccaattga tacaaaatac aaaaaacatc ttgatactag aaatgactat
     3001 aatcagttag tcacatatat tcatataatg aatttagacc aagcagatac aatgaaaggc
     3061 ggctttctgc aacctacttc cgatcccctt cctgcaaatc atgggtataa aaaaataggc
     3121 atattaagcg ggctaggagg agaaatattc acatatcatt ttttcattcc gcaagctgcg
     3181 tcaagttatt tggaatttgt agaacagatg agagaggtag aagcagcact acaaactact
     3241 tttcaataag taggaattat agaaatttta ctcaaaaaat cttcattaaa aaatctgact
     3301 aaataagttg agaaaacaga atatacttca atatccagtg atttataaat atcattcact
     3361 catgataccc agctccgtat cataagtgat gcggttttta tcattgatcc taaaaatctt
     3421 agcaagacaa aaagta
//
LOCUS       DACCCS010000136         3402 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.146, whole genome shotgun sequence.
ACCESSION   DACCCS010000136 DACCCS010000000
VERSION     DACCCS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.146"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(75..1073)
                     /locus_tag="IVK82_002822"
     CDS             complement(75..1073)
                     /locus_tag="IVK82_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP6393824.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1148..1444)
                     /locus_tag="IVK82_002823"
     CDS             complement(1148..1444)
                     /locus_tag="IVK82_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6393825.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1437..1730)
                     /locus_tag="IVK82_002824"
     CDS             complement(1437..1730)
                     /locus_tag="IVK82_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393826.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1876..2427)
                     /locus_tag="IVK82_002825"
     CDS             complement(1876..2427)
                     /locus_tag="IVK82_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP6393827.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2601..2900)
                     /locus_tag="IVK82_002826"
     CDS             complement(2601..2900)
                     /locus_tag="IVK82_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP6393828.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2927..3193)
                     /locus_tag="IVK82_002827"
     CDS             complement(2927..3193)
                     /locus_tag="IVK82_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP6393829.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
BASE COUNT          993 a          668 c          461 g         1280 t
ORIGIN      
        1 taaaaaaact gattctaaga aaattaaata atttttctca aaatcagttt ttctttgata
       61 aataatattt tcaattattt aatcatttga aaattgttag tcattccaat acgttgagaa
      121 attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt gtttccatca
      181 tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat tagatctttc
      241 atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg agaatcatac
      301 aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact tgaaattcct
      361 gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc ttctactact
      421 ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa ctgttgattt
      481 tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg cgttaaataa
      541 actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa atattctcca
      601 gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt tccatcttct
      661 gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc attaatcacg
      721 gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta gcttttatat
      781 tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga agctttattc
      841 gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga acttgcactt
      901 gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt tttacttttt
      961 ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt ctcagatgta
     1021 caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt cattatattt
     1081 ccccctttca atttctccct cacgtactta ttctatcaga caattaattg atctaaatgg
     1141 taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag cggtctcttc
     1201 cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat cctctacagc
     1261 gataagttct cattattttt gggctattag tattattttt cttagttaga gctatgagtc
     1321 caactatgat taccccaact gtcttaatta cttttccttt atctatattc ggatgcgcaa
     1381 gcatatattt atataaccaa tatccaccgt aaattaaacc acctaataca atcagcttag
     1441 ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct tgcagagaaa
     1501 ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat catgttttcg
     1561 attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat ctgttgccct
     1621 cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc ctcaaaaaag
     1681 tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat ctacacaccc
     1741 cctgttattt tttacataat gaaaatagca tattactggg tacattgata attcatataa
     1801 catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt ataagtaaca
     1861 tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca attgccattc
     1921 attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta tcaatacttt
     1981 ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt gttcataagc
     2041 cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga ttttcttttc
     2101 tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa gaaatacccg
     2161 aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag taaatcctaa
     2221 ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg gttctgtatc
     2281 tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc ctttgatcgc
     2341 ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa cttctgtttg
     2401 tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt ttgttcaagc
     2461 acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt catattcttt
     2521 ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa catttcgcaa
     2581 tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca aaccaatcgt
     2641 ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt aatgaaataa
     2701 actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt catcactaaa
     2761 cgtggtagat gaacatcttc aggaagcata cccatttcaa tttcctcttt ttccatcgta
     2821 acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt ttccagtact
     2881 tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag tattcttctg
     2941 gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga gacactgatt
     3001 tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct tcttgacgat
     3061 gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga tagtctaaag
     3121 gaaattgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt aattccaccg
     3181 gcaaactgtc cattactccc tttgtgacat aacgttatcc tttattttga aacatttctt
     3241 ttccctcctt tgccataaaa atagcccaat cttctattaa agaagattgg gcttcatgga
     3301 tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact acacaattat
     3361 taaagttttt catatctctt tgtttagtaa gtttttctaa at
//
LOCUS       DACCCS010000137         3398 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.147, whole genome shotgun sequence.
ACCESSION   DACCCS010000137 DACCCS010000000
VERSION     DACCCS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.147"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..276
                     /locus_tag="IVK82_002828"
     CDS             <1..276
                     /locus_tag="IVK82_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAP6393830.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="IVK82_002829"
     CDS             419..640
                     /locus_tag="IVK82_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6393831.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="IVK82_002830"
     CDS             656..1498
                     /locus_tag="IVK82_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393832.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="IVK82_002831"
     CDS             1561..3363
                     /locus_tag="IVK82_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP6393833.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
BASE COUNT         1228 a          469 c          703 g          998 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actgctggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaata
//
LOCUS       DACCCS010000138         3338 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.148, whole genome shotgun sequence.
ACCESSION   DACCCS010000138 DACCCS010000000
VERSION     DACCCS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.148"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            116..976
                     /locus_tag="IVK82_002832"
                     /pseudo
     CDS             116..976
                     /locus_tag="IVK82_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
     gene            988..1815
                     /gene="panB"
                     /locus_tag="IVK82_002833"
     CDS             988..1815
                     /gene="panB"
                     /locus_tag="IVK82_002833"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAP6393834.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1830..2678
                     /locus_tag="IVK82_002834"
     CDS             1830..2678
                     /locus_tag="IVK82_002834"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAP6393835.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2688..3083
                     /locus_tag="IVK82_002835"
     CDS             2688..3083
                     /locus_tag="IVK82_002835"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAP6393836.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
BASE COUNT         1303 a          347 c          665 g         1023 t
ORIGIN      
        1 gatattatga taaattaact atggatgata tttgaaaaaa acatactgta tatttgattt
       61 aatttttatt tttaagctca tatcgtaaga atgggacttg tagtctcact tctttatgga
      121 ggtgataatt atgaacgttg gttttattgg agccggcaag gtaggttgtt cattcgggaa
      181 gtatttccag gaacataaaa tacaagtaac aggattttat agtaaaagtg aagactcgtc
      241 attagcagct tcgaatttta catctagtaa acaatattta aacttaagag aattagtaga
      301 tgaaaatgat actattttca ttacgactcc tgatggtcaa attcaggagg tatggcaaga
      361 aattaaaaat tattaaatca aaaacaaatt aatctgccat tgcagtggtg caatatcttc
      421 agatatcttt tcaaatatac aggactatgg tgcttatggc tattctgtcc atcctatgtt
      481 ttcaatatca gataaatata attcatacaa aaaattaaaa aaatctttta taacaattga
      541 aggcgatgaa aaatatgcaa agtttttatg tgatttcttc aaaaagttag gtaatagtac
      601 tgtaattgta tctaaggaga ataaatctct ttatcatgcc gcatcagtag tatctagtaa
      661 ccttgtgcta ggacttatta atacaagcgt tacatatcta attcaatgtg gatttactga
      721 aaaaatggca attgaagctt tatatccatt aattgaattt aatattagaa atataaaaga
      781 aaaaggaatt atagaaagtt tgacagggcc agttgaacgt tgtgatattt caacaattaa
      841 aggtcactgt gaagttttat cagatgaaga cagagcttta tatatgctat tatcaaaaaa
      901 tgtattagag atagctatgt taaaaaatat taatagagat tatagtgaat tagaaaagta
      961 tttaggagag agttaggaga gaaatagatg aaaaatacag cagttacatt taaagaatct
     1021 aaattaagaa atgaaaaatt aacaatgctt acagcttatg actattcaac agcaaaaata
     1081 attgatgaag ctgggataaa tggaatatta gtaggagatt ctcttggtat ggtatgtcta
     1141 ggacatgaag atacactttc agtaacaatg gaagacatga ttcatcatac tagagcagtt
     1201 acaagaggag ctaagaacac tttagttgtt gcagatatgc catttatgtc ttatcaaact
     1261 tcagtatatg attcagtagt taatgcagga agattaataa aagaaggacg agctcaggtt
     1321 gttaaattag aaggagggat agaagtatgc gataaaatag aggctatagt aaaggcttct
     1381 atacctgtta tggcgcatat aggtttaaca cctcaatctg ttaatgcatt tggaggattt
     1441 aaggtacaag gaaaggacaa ggaagctgct aaagaattaa taagagctgc taaagctgta
     1501 gaaaaagcag gagcattcgc tgtagtgtta gaatgtgttc caactaagct tgcagagtta
     1561 atttctaaag agatatcaat acctacaatt ggtattggag caggtgcagg gtgtgatgga
     1621 caaattcttg tatatcaaga tatgttgggg atgtatagtg attttacccc taaatttgtg
     1681 aagaaatatg ctaatttatc agaagagatg aataaagcat ttacaaaata tatagaggaa
     1741 gtaaaagatg gagtttttcc aggtccagaa catgggttta aaataagtga tgatgtttta
     1801 gaaaaattat attaaaaggg tcaagaaata tggttatatt taaaacagtg aatgatgtta
     1861 gaagtcaagt aaaagagtgg aaaaaacaag gcttaaaggt aggattagtt cctactatgg
     1921 gatatttaca tgaaggtcat gaaagtttaa ttagaaaagc atcaaaagaa aatgataaag
     1981 ttgttgtaag tatctttgta aatccaactc agtttgggaa aaatgaagat ttaggtagtt
     2041 atccaagaga cttagaaaga gatatagaag tatgcacaag aggtagagca acagcaatat
     2101 ttaatcctga agttgaagag atgtattgtg acaatgcaag tacttttgtt aatataactg
     2161 gattaacaga aggtttatgt ggagctagta ggccaataca ttttagaggg gtttgcactg
     2221 ttgtaagtaa attatttaac ataataccag cagatagagc ttattttgga gaaaaggatg
     2281 cacaacagtt agctgtaatt aaaagaatgg ttagggacct taatattgat atagacgttg
     2341 tagggtgtcc aattataagg gaagaggatg gactagctaa aagttctaga aacacatact
     2401 taagtttaga ggaaagatct tcagctacta ttttaaataa atctttaact ttagctaagg
     2461 aagctttaaa taatggagag agagacagct taaaaataat tgagataata agtaaaaata
     2521 taaatactta taatttggca aagattgatt atgtagaagt tgttgattct ttatcattac
     2581 aaagagtaaa ttatattgaa aaatcagttt tagttgcaat agcagtattt ataggaaaaa
     2641 caagattaat agataatttt acatttgaat tacaataggg ggctataatg aaagttacaa
     2701 tgttaaaagg aaaaatacat agagctacag ttgaacaagc tgagttagat tatgtaggaa
     2761 gtattactgt tgatatgaat ttattagaag cagcaggaat atatgaatat gaaaaagtac
     2821 aaatagttga tgttaataat ggaaatagat ttgaaactta tacaatagct ggagagagag
     2881 gaacagggat gatttgcttg aatggagctg cagctagatg tgtatcaact ggagataaaa
     2941 taattttaat ggcatattgt gaattaaatg aaaatgaggt taaggatcat agacctaagg
     3001 ttgtttttgt cgatgataat aataaagttg aaagagtaac atcatatgaa aaacatggaa
     3061 ggttatcaga tataattctt taggttttgt aatgttattt atatatagct agactattat
     3121 tgaaatattt attgattact aattggatag atgtcaataa agtagacaca aaaagagaag
     3181 atacagactg tagacaaagt ccattttatg tgaatcggct acactagact agacagaaaa
     3241 aataaggtgt gtagaataat aaaaaacaca ctggaggatt tttcatgtca agacgtcaac
     3301 gaagaaccta ttcaaaagaa ttcaaacaac aaatcgtc
//
LOCUS       DACCCS010000139         3292 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.149, whole genome shotgun sequence.
ACCESSION   DACCCS010000139 DACCCS010000000
VERSION     DACCCS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.149"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            37..588
                     /locus_tag="IVK82_002836"
     CDS             37..588
                     /locus_tag="IVK82_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAP6393837.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1264..1530
                     /locus_tag="IVK82_002837"
     CDS             1264..1530
                     /locus_tag="IVK82_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393838.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1588..2100
                     /locus_tag="IVK82_002838"
     CDS             1588..2100
                     /locus_tag="IVK82_002838"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6393839.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2097..3134
                     /locus_tag="IVK82_002839"
     CDS             2097..3134
                     /locus_tag="IVK82_002839"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP6393840.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1213 a          555 c          653 g          871 t
ORIGIN      
        1 agcgagcaag acaggaacga gagaacaaac atactcatga aaagactaga ggcttcgacc
       61 tcgaacttta ataataagtt agatgttttt gaagaaaaga ctaagcatgc aagattaaac
      121 tttgaaacaa ctgcaaagca ctatattaaa cgacttgatg aagataattt gaaattagat
      181 tttcaacaag ctattcaaga tgaattaagc gatactaaag acgaaatacg tgaagtgacg
      241 aaacaagcga gagaagaaac aaaagagtat aaagaaatct tagaaagtaa aataaaagac
      301 cataacaaag tggttgataa atcgaataca gcgctaaaag tgatgacaaa aggcgttaca
      361 aatatatttt ttgtgctaat tatttttgta cttgtaatgc ttgtgacagg tccgataggt
      421 catttctttg gtattgaaca tttatatagc tttattaatg gttttattga tgaccacgaa
      481 agtgcatggc gatatttgat gttgatactt tatgcagtac cctatgtatt cttcgctatc
      541 attctgtggc tcatagcatt gttttttaga gtatttaatg acctttaggt agatttatat
      601 ataaaaaggg ctaaatacgc aaaatacccc cttaaaatgc aaaataaatg caaataaggg
      661 ggtaactcct tatgaaaaaa caactaatag tattataaca cgtttttatg ccgagaaaac
      721 ttattggttg gaatgggcta tgtgttagct aacttgttag cgagttggtt ggacttgaat
      781 tgggattaat cccaagaaag taccaactca acaacacata aagccctgta ggttccgacc
      841 aataaggaaa ttggaataaa gcaacgaaag gagttgagta aaatgagtga gaaaacgaag
      901 aaaatcattc gagtatcatt agctttttca caagtagtag tgcaatcatt gatagtttat
      961 tatacttacc agcaatataa attatctaaa gagaattaag ttttgattat gtaataagtt
     1021 ttggtactaa aaaagacttg atctaattag accaagtctt ttgttagtgt tatattaata
     1081 acaaaataaa aataagaagt cgctcactcc ccgaccaaag tttgtgagca aaataaacag
     1141 tcgatgtttg tttcttaatt gtatatcttg atattaaacg atttaagatt attcttcaag
     1201 atatatattc gcaaatgtaa ttggaaaagc gacttcttaa acaaaattaa ggagtcgctt
     1261 tttatggcaa aaataacgaa aaatgagcaa gaaaatttgg tggaaaatta cacaaaaaaa
     1321 ccggcgtact ctaatagccg gttagacgca catttcgtgt gcacttctaa tccgaaattg
     1381 agctttgacg ctatgacgat agtcggaaac cttaataaaa acagcgctaa aaagctatcc
     1441 gattttatga gtttagatcc acaaattaga ctttgggata tacttcaaac aaaatttaaa
     1501 gctaaagcac tacaagaccc cctatgttag gctagttgtc gtataggata ataagttata
     1561 ataaagacta tacggaggat gattaccatg acaaataaaa acaatgaacg ttactctacc
     1621 gaactgaaag cgacagccat caagaaaatg atgccacccg aaaatagggg gattcgccaa
     1681 ttaagtcaaa ctttgggcat tccagaagcg actctttgtt cttggcgtaa aaaagctaga
     1741 attcaaggga atactacacc cggaacgaaa aaaaatgtcg aaaaatggtc aagtgaagat
     1801 aaatttttaa ttgtgatgga atcattctca atgaaccaag tagaacttgc ggaatattgc
     1861 cgaaagaagg gcttatttaa agaacaaatt gaggcttgga agaaaacgtg cttatctgcg
     1921 aatgaacagg aagaaaatcg aacgagagaa cttgcaactg aactaaaaga agagaaaaaa
     1981 caagcgcgtc aattagagaa agaattacgt cataaggaaa aagcattagc ggaagccgct
     2041 gctctcttat tactaagaaa aaaagcacac gcgatttggg gggaccaaga ggaagaatga
     2101 tttcatcttc agatcgcaaa caagctgttg aacttattca agaagccaat cgcaatgggg
     2161 cccgtctcac ctatgcctgt aatgagctga atattagcgt tcgaacctac gaacgatgga
     2221 cgcgtgaagg tacgatagcg catgatcaac gccctttggc gaaacgtcct gtacccaaaa
     2281 ataaactgac ggatcaagaa cgcgaaaaaa taatagaaac cgtttcaaaa aaagaattta
     2341 tgaatttgcc acctagccaa attgtaccaa aacttgcgga ttgctcgatt tatattgcat
     2401 cagagtctag cttctatcgt gtattgcgag aaaaaaatat gcaacatcac cgtggccgaa
     2461 gtcaagtttc acaaaaacgc ataccgccaa gtcaccttgc gacgaagcca aatgaagttt
     2521 ggacttggga tattacttgg ctaaaaggtc caataaaagg actcttttac cggttatatc
     2581 tcatcattga tttatttagc cgtaaaatgg taggctggga gatttgggaa tcagaagaag
     2641 ctcgtcatgc cgaaaaactt attaaacaga cggtgcttaa ggaacgtatc caaggacagc
     2701 cgttggtttt acactccgat aatggcagcc ctatgaaagc cgcgactttt caagtcttac
     2761 tcgaaaaatt aggaattcaa agttcttatt caagaccacg agtgagtaat gacaatcctt
     2821 attcggaagc ggctttccga acgttaaaat atcgaccaga atatcccaat aacggcttta
     2881 aaacaataga tgatgctcga aaatggacga aagagtttgt cgattggtat aacgattctc
     2941 atttgcatag tgggattcaa tttgtgtctc ctgcagcgtg tcatgaagga cgacatctcg
     3001 ctattttaga gaaacgaaag caggtttacg aggacgctaa aaagcgccat cctgaacgct
     3061 ggtcaaaagg tgttcgaaat tggactccac atcaatcggt ggcgttgaat cctttaaaag
     3121 aaacagaaag ttaacaggag cataatcttt caagattatc aaataaccct cgtcacccaa
     3181 taggggaaaa agttattctt cccctattgg ataacgaggc caaccgacca tagggaggtt
     3241 ctgctttttg ataggtggaa aataaaaata cgacaactat attgacaaac ac
//
LOCUS       DACCCS010000140         3290 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.150, whole genome shotgun sequence.
ACCESSION   DACCCS010000140 DACCCS010000000
VERSION     DACCCS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.150"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..66)
                     /locus_tag="IVK82_002840"
     CDS             complement(<1..66)
                     /locus_tag="IVK82_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP6393841.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYL"
     gene            complement(140..421)
                     /locus_tag="IVK82_002841"
                     /pseudo
     CDS             complement(140..421)
                     /locus_tag="IVK82_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            883..2265
                     /locus_tag="IVK82_002842"
                     /pseudo
     CDS             883..2265
                     /locus_tag="IVK82_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2501..>3290)
                     /locus_tag="IVK82_002843"
     CDS             complement(2501..>3290)
                     /locus_tag="IVK82_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6393842.1"
                     /translation="SFPTTTPEETMPLVIDFFKQYQADLAGIGIGSFGPIDIHRDSAT
                     YGYITSTPKLAWQNFDFIGTMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYT
                     IGTGVGGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIE
                     KRLGKKGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHD
                     AFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT         1108 a          628 c          585 g          969 t
ORIGIN      
        1 caggtagtaa ccaacagcga caataatgac gtcttttttg aattgtttgc ctttaaaatg
       61 attcattact ctgtcctctc tgtctttttt ctcaatttta cactaaaata gattttttgg
      121 aaaactttgc aacagaaccg aaatgagtat agtatgaatt agtaatttgg ttttcatcta
      181 agtttagctt tgatttacta aaattgatat ctcgatacat gttcaataca tctaatacaa
      241 atcgagaatc ttctttcgga aatggctcag aaagatcact ccaaatttca tcatcataat
      301 gatactcgta accttctctc aatatttcga tttttctttc ataataagat ctgttttggt
      361 cctcaaattt agccaataac tcaaattgat ttactagtga caatctctga aaactgtcca
      421 ttaaatgttt cttcctatct tgttcattaa caaaaaatta taagccctta caacgaatat
      481 tatatattaa atttaattgt aggtgaacac gaattttata aatatttatg gtgttaatga
      541 caaatgatat ccgactcctc ttcatacgtt ctggactatt tgctaataga ttaaaatttc
      601 agcatctatt tctgaccata aataaatgag ctgccgaaaa gctgccaaat cagacttgaa
      661 aataaaggat caaaaacttt taagtttctg accccgtaac tcacacttca aaatcttact
      721 taaaaaggag tctgaaagaa aaaaagagta ttcaaaattt ttttctaact agctacatca
      781 catctatgca ttgacactca aagaaccaga ataagtcatc agaaagcaag ggggtgcgga
      841 cataatcctg gacgaaaaaa taaacttagg aggaatgtcc gtgtgttttc ccacaaagag
      901 cgtgtaaggg caattcagtt atttttaaaa tacgattgct cttatgctgc aaccattcga
      961 gaattagggt atccgtcgat tggtgcacta cggaaatggt ataaagagta tttggaatcc
     1021 ggagagctcc atcaggagca ccgaaaaaaa tcaaagtatt ctgaagaaca aaaacgaata
     1081 gcggtgaatc attattttga atatggtcaa tgttatgcac gaaccattcg catgcttggt
     1141 tatcctaata gagagtcctt gcggcagcgg tgtgaagaac ttgcaccagg agctagaaaa
     1201 ctccgaaaaa gtgcggtaaa gttaacacaa gatcaaaaag aagctgtgtt gaaaaaattc
     1261 tataaaccgc aaactaatag gaaaaattta gccgaatctg gaggaattag tagagttact
     1321 ctatatcaat ggaaaaacac ggttcttgga aaggattttc cattgtgtat gactttaaaa
     1381 aatcaggaaa aacaaaaaga gctactttta aaggaagtag aagacttaca aaaacaagta
     1441 caccaattac agctcgaaaa agcattactc gaaggagcgg ctgaactgct aaaaaaagaa
     1501 aaaggcgtca atcttctttg tctatcaaat caagaaaaaa cgatattgat tgatgcctta
     1561 cgaaatcagt tcaccttgaa agagcttctt caacagctcc agtttcctaa aagcagctat
     1621 ttttatcaaa aacaagcact tgagaaacca gataagtatt acaaagaacg tcaaattaat
     1681 tatcacgatt ttcaatcata acttctgtgc gtatggttac cgtagaattc accaagcttt
     1741 aaaaaatatg ggtaaaaagc tttctgaaaa agttgtacgt agattgatga ctgaggaaaa
     1801 tctatttgtt aaattttcta gacgaaaaaa gtattcttcg tatgctgggg aaatttctcc
     1861 tgcacaccct aatttattaa atcggaattt taaagcaaag aagccaaatg aaaaatggtt
     1921 aacagatatt actgaattta gaattctcgc agggaaaatc tacttatcac cacttgtcga
     1981 ttgttacgat ggtgctattg ttagttggac tattggaaca aagccaactt gcttcactct
     2041 atgtctaaaa agggttattc accagataat tcagcgtgcg aaggtttctt tggacgatta
     2101 aaaaacgaga tgttttatca acgtgactgg aagaatacaa caatcaatca gtttataaac
     2161 aaattggatg attatattca ttggtataat aatcaacgaa ttaagttatc tttaggaggg
     2221 ctaagccctc ttcaatatcg gaagaagcaa agatatattt gttaaaaagg tccaaaaaaa
     2281 atatccgcac cccctttttt ttgtaaaaaa ctattgcaca atacaagtag cctacggcta
     2341 tcgaaatttc cataactttc gagcaagaac ttatctcatt caaggcttga tcttccaagt
     2401 aaaacaaaaa ccagttaagc actctgccta actggttatg actaaatttt cagttgtatc
     2461 tttctctacc aacactaaat gtcgaagagc cttttttcgt ttatgaatgt aagaccgctt
     2521 ctcgagccaa tgccaaacaa ccgagcgttc cagcgttgtc ttctaatgct ggggtcacga
     2581 tatatttgct taactcaggt gtttcgacat acccattgac caattttgca aatgcgtcgt
     2641 ggacgttttg cagcatatgg cgttgtttca tcacaccacc gccaaaaatg atcacatctg
     2701 gagaaaacat caaggttgta ttgtaggcac attgagcaat ataggaggct tcgatttccc
     2761 aataaggatc atcttctgca acctcttgtc cttttttccc taagcgtttt tcgatggcgg
     2821 gtccagcggc catcccttct aaacagttgc catgaaatgg acagctgcca gcaaagtcat
     2881 cttttggatg agggacgacg agcatatgcc ccatttctgg atgactaaac ccttcgataa
     2941 agcgcccttc ttgtaaggca ccgccgccaa cacccgtacc gatcgtatag taaacgacac
     3001 tgctcaatcc tttgccactg ccgaagacat actcaccata ggcagccgca ttgacatctg
     3061 ttgtccaaga aatcgggatc ggaaattctt ttttcatggt accgatgaaa tcaaaatttt
     3121 gccatgccaa tttcggggtc gaggtaatgt acccataagt ggcggaatcg cgatggatat
     3181 cgataggccc aaatgagcca atcccaatcc ctgctaaatc tgcttggtat tgtttgaaga
     3241 agtcaatgac taaaggcatt gtttcctcag gtgtcgttgt cggaaagctc
//
LOCUS       DACCCS010000141         3221 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.151, whole genome shotgun sequence.
ACCESSION   DACCCS010000141 DACCCS010000000
VERSION     DACCCS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.151"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            50..1438
                     /locus_tag="IVK82_002844"
     CDS             50..1438
                     /locus_tag="IVK82_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAP6393843.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>3221
                     /locus_tag="IVK82_002845"
     CDS             1776..>3221
                     /locus_tag="IVK82_002845"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP6393844.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEEE"
BASE COUNT         1185 a          505 c          583 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaagaaga a
//
LOCUS       DACCCS010000142         3168 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.152, whole genome shotgun sequence.
ACCESSION   DACCCS010000142 DACCCS010000000
VERSION     DACCCS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.152"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..863)
                     /locus_tag="IVK82_002846"
     CDS             complement(<1..863)
                     /locus_tag="IVK82_002846"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP6393845.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTT"
     gene            complement(907..1737)
                     /locus_tag="IVK82_002847"
     CDS             complement(907..1737)
                     /locus_tag="IVK82_002847"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP6393846.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            complement(1752..2690)
                     /locus_tag="IVK82_002848"
     CDS             complement(1752..2690)
                     /locus_tag="IVK82_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP6393847.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            complement(2857..>3168)
                     /locus_tag="IVK82_002849"
     CDS             complement(2857..>3168)
                     /locus_tag="IVK82_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP6393848.1"
                     /translation="FISLAVLFPAMVHVSSIWTAVLASFVLSVLNMFVKPILTILSLP
                     FTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILMSLVNVIVSEHNSR
                     K"
BASE COUNT          942 a          723 c          564 g          939 t
ORIGIN      
        1 gtagtattca caccttcaac gaccattccc atattggcta gcacctcttc taggggctgc
       61 ccttgtccta acaagttgcc tgcgcgccag ttcctagaat gaacacttgt gcaagtaacg
      121 actagatcgc ctattccgct taatccgata aaggtcagcg ggttggcacc cattgctaca
      181 cctaaacggc tgatctcagc taatccgcgc gtcatgattg ctgcctttgc attatcgcca
      241 aaccctaagc cgtgaatagc gccagcacca atagcaataa tatttttcaa agctgccccc
      301 atctctacac cgatcacatc aggattggta tagatccgaa aataattatt catgaacagc
      361 tcttgtacat attttgctgc ttttgcatct gtacttgcag ctgtgatcgt agtgatgtca
      421 tgttttgcta cttcttctgc atggcttggt ccagaaagaa caacaacagc ttttcttttt
      481 tctgctggga tttcctctaa caagatgtca gagatccttt tatgtgttcc ctgttctaat
      541 cctttacttg catggatgat caccggcttt gtattcaatt taggcactag ttcttttgca
      601 acagaacgta ttgctttcgt tggaatgacg aatagcaccg catctgcgtc ttttactgca
      661 tcttccatat tggtgaatgc ttggatggaa tcgggtagat aatgatctgg taaataacgt
      721 tgattcgtgt gttgggtgtt gatttcttct acttgtgaag catgatgtcc ccaaatccga
      781 acgtcatgtc cgttttcggc gaggacttgt gccaaagcag taccccaaga gcctggtcct
      841 aaaaccgcta ctctataatt cattttacca ctcctgaaat ttttttcatt tttaacgctc
      901 taaaaatcag ataatttgtt gattttttcc taatgagcgg tcataatccg gtaaatttcc
      961 tttttttctg cgccaaataa atagaatcaa tgtaccgaca aataacacag cagataataa
     1021 ttgagaaata cggatcacac ctaaaaagta aagactgtct gtacgtaatc cttcaataaa
     1081 gaaacgcccg aaactatacc acattagata acataaaagg acttcgcctt ttttcaaaag
     1141 gtgtggtttc tttcgtaaaa acaaaagcaa aataaatcca atcaaactcc aaagtgattc
     1201 ataaagaaat gttggttgac gatagatccc ttcgatatac atattatcaa tgatgaattg
     1261 cggtaaatgc agactttcta agaagcttcg cgtcgtgtct ggtccatatg cttcgtggtt
     1321 catgaaattt ccccaacgac cgattgcctg tgctaataat acacttggtg cagcgatatc
     1381 taaaaaagtc cacaaagaaa taaaatgata tttagtaaaa aaatacagcg tgatcgcgcc
     1441 tgcaatcagt ccgccataaa tagctaagcc tcctgaacgg atattgaata tttggattgg
     1501 atcagctata tactggggaa gatcaaataa aacataatat aagcgagcgc caataatgga
     1561 gataggcaat ccccacaaca taaaatctgt cacatcatct gcttttaatc ctacccgaac
     1621 agcctctctg ctgctaagcc acatagcgat aatgatacct gatacgatga tcaatgcata
     1681 ccagtagata ggaatcccta caagctcaaa agcaactcga ttgatttgtg ctaacatatt
     1741 gtcactcctt gctagtcgtt gcctgagttt tcttcaatca agcgacttaa tctttcttca
     1801 aatgttttcg ttgcgtcaaa gcccattgta cgtgcacgga aattcatcgc ggctacttca
     1861 atgatgattg caacgtttcg tcctgttttg acagggattt ttatttgtgg aatgcccact
     1921 tccgcgattt caacggtcgt gtcttcagag cccagacggt cgtatttctt atcttttgcc
     1981 catgcttcca gataaacagc caactggact tgcatagagc cacgtaccgc actcgcacca
     2041 aaaagattca ttacatcaat gatcccgatt cctcggattt caatcagatg ttccaggatt
     2101 ttaggcggtt ctccgatcac cgttaattca tcttgctgat aaacatctac tcgatcgtct
     2161 gcgatcaatc gatggccgcg tttgatcaac tccaacgctg tttcactttt tcctatccca
     2221 ctgtcacctt gaatcagtac acctaatccg taaacatcca ctaaaacacc gtggacactt
     2281 gttcgagggg caaggcgacc atctaaataa ctggataatt ctcctaataa acgagaagtt
     2341 gagatgggag aacgcagtac agctatccca ttttcatttg ctgcctggat catttcttct
     2401 ggagctggta atcctcttga aataataaat gcgggcatat cttcactaca taatcttctc
     2461 atgatgatca aacgctcttc tgggatcatc cgttccgcaa aactgatttc tttactgcca
     2521 aatagctgta aacgattatg ggaataataa ttaaaataac cagtcaactc tagtcctggg
     2581 cgtgagatgt ccccagttac gatgacacgg tctaagcttt tctcgtcgcc tgtcacgatt
     2641 tccaaagata attttttcac taattggcta acttttacaa cttctgacat tcatgctacc
     2701 tcatttctat tagagtgttc tctatcattt tatcattgtc tagccaaaag tactaccaat
     2761 taaaaaaaac gtttttattt aattaccaat gtaatagaaa acgcattcct gattctaaat
     2821 aaaaaaactg cgccgatagt tcggcacagc tttcttttat tttctactgt tatgttcgct
     2881 gacaattaca ttgacaagcg acatcaagat agctactaac aacgctgcac cgaatgaaga
     2941 aaaactaaaa tgttctgctc ccacaaaaaa ggaggtcatt ttaagaatcg ctgcattgat
     3001 aacaaacgta aacaatccca atgtgactaa tgtaaagggc agggacagta tcgtcaaaat
     3061 cggtttgaca aacatattca acacagacaa tacgaagctg gcaagaacgg ccgtccaaat
     3121 actgctcaca tggaccatcg caggaaaaag aacagccaat gatatgaa
//
LOCUS       DACCCS010000143         3120 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.153, whole genome shotgun sequence.
ACCESSION   DACCCS010000143 DACCCS010000000
VERSION     DACCCS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.153"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..556
                     /locus_tag="IVK82_002850"
     CDS             <1..556
                     /locus_tag="IVK82_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP6393849.1"
                     /translation="WTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKE
                     KWNALKDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQ
                     TMTNLVKTPSVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITT
                     KIKTELTRQMKRDAGAVGGTLYAT"
     gene            546..1286
                     /locus_tag="IVK82_002851"
     CDS             546..1286
                     /locus_tag="IVK82_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6393850.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1283..>3120
                     /locus_tag="IVK82_002852"
     CDS             1283..>3120
                     /locus_tag="IVK82_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393851.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1169 a          385 c          610 g          956 t
ORIGIN      
        1 atggacaaat ctaaaaaatg gtgtttcaaa tgctattaat cgggtaaaag aaatctttaa
       61 ttcgttaagg gaagtcaatt tatttgaaat tggcaagaac atcattgatg gacttataaa
      121 tggtgtaaaa gaaaagtgga atgctttgaa agatactatc aaaggtattg caggtaatat
      181 caaaaattgg attaaaggag ctttagatat tcattctcca tcaagatgga tgcgtgatat
      241 ggttggtaaa aatattgttc aaggtattat cgttggtatt gataaagagc aaagtaagtt
      301 agatcaaacc atgacaaatt tagtaaaaac accgtctgtt caacctgtag taaatggaat
      361 aagtcaccta ccaattacac aaacaaagca aaatatatct gctagtgaaa caaaagaaat
      421 tcatttacat ttaaatgttt atggagattt acctgattca atggttcgga agattaccac
      481 aaaaattaaa acagaattga caagacaaat gaaacgagat gctggtgcag taggaggaac
      541 attatatgca acttaagaga ggacaatttt ttattaatca acattattct tctgaattta
      601 atgtgtatat tcaaaacagg cctgcttctg tttcagctag tcgagtaatt gaattgagag
      661 agcgagaagg aaatgattct atcattattg ataaaggcta ttacagaaat gtgactagaa
      721 agattgagtg ttactataaa gcaccttcaa ttgatgtggt gcaggagtgg gaagatcgaa
      781 tcactgaatg gttagatatg tcgtcttata gtgattttat tctttactat gatgaacaat
      841 atatttatca agctgtagta atagaaggac ctgaatttaa aggaacaaga aaaacaggaa
      901 atattgtccc ttttgaattt acagtgagca ttcgaccatt taaggaaaat tacaatggta
      961 gatttactat tcgacaaaca gaaacttttg aaatttataa cccagagaag tattcttcaa
     1021 aaccgcttat taaattgagt ggttctggag atgcttcttt ttatattaat aaggacaaat
     1081 atgatttgaa atcgttggac agagaattat ttatagattc taaattagaa gaggcttacc
     1141 gaaaattaga tggtaattta gaacatcagg atcaagtcac tttattttta gattttccat
     1201 ttttatatcc tggaaaaaat gaaattaaat ggacgaaaaa tattcattca tttgaaataa
     1261 tgcctaggtg gtggagaaaa gtatgaaacc aagaatatat agtcctactg aaacagattt
     1321 ctcaacgaat ggcttaggaa ttttaaagga ttgtacaaga tgtgaaatat atgaagtagc
     1381 taatggaaaa tatgaattag agttggatta tcctttagga actagatttg atgaatattt
     1441 tgaaaatgac tatcaaataa aagcaaagcc aaacgatcaa gaagagtatc atatcttttt
     1501 tattgatgat aaagatatag atactttttt aaatacagta actatttatg ctcagagtcg
     1561 tacaaatcga cttggaagac gggtagtcac tcttgcggaa gtagactcta aaactggtca
     1621 agaagcgata tcaattattg aaaccaaaat ggataaaaaa tctgacatac gactttattc
     1681 tgatattacg gccgtttcta gcacaatctt tgaagcgaga aatgttttaa attgtattgc
     1741 tggtgaacaa ggatcattgc ttcaatattg gggtggagaa attaaacgtg aaccatttaa
     1801 gctttctttg ttaaagcgaa gaggtcgcaa taatgttgga acgattcggt atggaaaaga
     1861 tatgtctggt ttaaaggtca aattagattg gacaggtata aaaacaagac ttattccata
     1921 tgctgatcct caaagtgagg taggtacgac taatcgaatt tatggttcgc cagtagatag
     1981 tgcctatatt aataactatc ctgatgtgta tacagagcat gttcagttta cagaagaaca
     2041 aggagtaaaa gacattaaga gcttaaataa aatagctaaa aattacttta aaacgatcaa
     2101 tcctggctgt gataagccta aagtttctat tacggttgaa tttgataagt tgactgatag
     2161 tgaagaagcg aaagaatttg cgaagattag aaattatggt ctgtttgata catttaaaat
     2221 atatcataaa aaatataata tttatcttga atcaaaggtt agtggactac aatacgattc
     2281 tttatctgaa aaaactttga aattagaagc aggagacatt caagttgctt tttatcagca
     2341 acaagctgta actattcagg ataaattaaa agattatgca acgaataatt atatgagtga
     2401 ttttaatgat tatgtttctt caatgattac aggtcaaggc agtgcaggag gatatgtaag
     2461 tttatggcca aaagaaaaac cttccaacat ctttattatg gataatccag atttagaaaa
     2521 ggcaagagaa gtactaagaa tgaataaaaa tgggattgct ttttctaaga aaggatggcg
     2581 ggggcctttt acttcagctt ggacattaga tagtatattt aatgccaatt ttatacaaac
     2641 tggattaatc aaagcagata tctttcaaaa ttcttttaac aagactggag atgtattgaa
     2701 attagttaat ggactactcc aaatttggaa caacaaaaag aaaataatgg agttaactaa
     2761 aaaaggaatg gaattttgga attctaaggg ttcaattgga acgattggaa caactgattc
     2821 tgctggtaat ccttttcctg gggcttctac tccaacccct attgaagata attctttagt
     2881 tattcgtaca aatggagacg gcaaatatat tttgatttct cctaaagtag gaaaaggatt
     2941 agttttatta ggaaatggta aagcaattta ttttggagac ttagatgtac aaggaaaact
     3001 tacagttaaa ggaaaagaaa taattcctgg acaaggtggt ggtccatcgg gaggtggaga
     3061 atctactggt gggtatccta gtgaattaaa aacagatgca gaaaagagag cttggagaat
//
LOCUS       DACCCS010000144         3007 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.154, whole genome shotgun sequence.
ACCESSION   DACCCS010000144 DACCCS010000000
VERSION     DACCCS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.154"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(276..692)
                     /locus_tag="IVK82_002853"
     CDS             complement(276..692)
                     /locus_tag="IVK82_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393852.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(695..979)
                     /locus_tag="IVK82_002854"
     CDS             complement(695..979)
                     /locus_tag="IVK82_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393853.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(1679..1774)
                     /locus_tag="IVK82_002855"
     CDS             complement(1679..1774)
                     /locus_tag="IVK82_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP6393854.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            2427..>3007
                     /locus_tag="IVK82_002856"
     CDS             2427..>3007
                     /locus_tag="IVK82_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP6393855.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALREL"
BASE COUNT          958 a          477 c          565 g         1007 t
ORIGIN      
        1 aaaaaaatac ttcaatgatc ttaaagtcac aaaaataaaa gcaaaaaagg gcaataaaat
       61 cgccaaatta gaatttactt tttcaagcct aacgtcagat ttgccaaaga tttctctgcg
      121 taactggtta gaagaataaa taaaaaagaa ctcgcccccc ttacattaag caagtagaca
      181 agtcccttac aaccgactaa gattgctagt acattctagc atattgtatg gggcttctaa
      241 ataaaaaaca gataggaata actcctatct gttttttatc gttttagaaa atttgttggg
      301 tcgaaatttc tagaagtgac tgtttgactg gtttcttttt gatgattctt tggtcgattt
      361 cgatttgatg gatttagttt agcttcgatt ttttctttca cttcttgata tactttagaa
      421 tttgtgccaa taagaacgcc tgtatctttt agatataaat cagcaaattc tgcgatgatt
      481 tcagtcatga tggaaatttc ttttcccata gcatttagtt ttgcctgatt gctggtagtg
      541 gtcgtgttgg tatcgggttc ataactcata cataaaaagc gaatggcttc cgagtagtta
      601 ttgatatctg gatttttttc gatgatggaa tctaaaacgt caacatgact agggtacaaa
      661 gtaacatgtt tttggacaga agtttttctc atggttatat cattcctccg ataagcattt
      721 caatgatttg attatttgtg tagacatact cgcttaatat ttgagtattt tctgtttgga
      781 tagcaataaa tttatcgagc aataaatcga attggtcttt tttgtaagtc gtaagttgac
      841 ggtcaaaatc actttcgatt aaaactttta gatatttatt tcgactccat ttttctcctg
      901 ttttattcgt gagttcttgg caacgtttgt ctattgttgc aacaaacttt ggatcaatat
      961 tccgaactaa aatgtccata aaaaatctcc ttttgcaatt atgtataatt tatgtatacc
     1021 atacaaatac tatatttgta tagctgtttt ttgccaaaat gctatacata agctacccac
     1081 gatggcgaaa aagtgatatc aaattttttt ctgagaaacg tgataggcac agcctatcta
     1141 cccacttttt ttagaaagtg ggtacacgtt tccttatgct ctttaaaatt cgggggggag
     1201 tgctgcacgt gtaatcgata accaaattta acagacgaaa aattgttgga atataaaaat
     1261 atgacacccg aaagaaaacg atcgttggaa aaattaatgc aactagtgaa cgaagacggc
     1321 ggaatttcag acgcagcttt tgacgcatgg agcgaatttc atggagaata taattaagtg
     1381 ggtttaaatg gctctaaaat cgcttctaag agttttttag cttggaccaa tgaaatacac
     1441 caaaaactaa ataaaacgtc ttaaatcgaa aaatacgagc tctcagagcg actttatttg
     1501 taaatgttgc atataagaaa taattacgga ttttagactt ttgtaggata tttttgaaat
     1561 aaatatttat ttctgttata atttgattgc aaataagtga acacgaaaca cacgaaatcc
     1621 ccagagaggt cggattctct ggggattttt ttgtgggctg agtcgcctta attaagtttt
     1681 acttcttgtt tcggttattc aaccagtaac tgaacaaagc gcttaaaagc ccaacaagaa
     1741 gggagcaaat aaagttagtg aacacgaaac acacctcctt ccagtcgcaa gactgtcgga
     1801 taggcgacaa tcaattatac aataaaaaag caaaaataag cctaagtttt ttattgcctt
     1861 ggggttgtta gggcttgccc tgaccgcctg taaggcgctt tagaacgaag gacatagttt
     1921 ttatcgaatc ttgtcttaga acggcttaga cgagctctca ggacgccaca aaggagtccc
     1981 taatgaatta tttttggcga aggagcctaa aggcgactgc tgcaagtaaa atgtgagagc
     2041 gtagcgatca ctccttctta cagcgaccat agggagctag attggcagtt taatatcatg
     2101 cgcgaacctg tttattttaa aaagtgggtg attttttggc gggaaatttt gctcgcgcgc
     2161 gcgcgtttat tttatttatt ttttaaagat tttttagaac cctttttagg gggcagttga
     2221 gccttactct cccaacggat acagaagggt actaacaact ttttatctga gtactaacaa
     2281 ctttttatct gagtactaac aactttttat ctgagtacta acaacttttt atctgagtac
     2341 taatatcctt ttgtataata acaaccaacc tatttacgag ttggttgtta aaggttatta
     2401 tttatttgta aggggggaag aaaggaatgg atgaaaaaac ggaattaaca gtcaaatatc
     2461 aaaatgaatt gaatttagta ccgctgaaaa atttcaatgc taagcagatg gatttatttt
     2521 ttgcattatg cgctcgtatg aaagatcgtg gtttgaaaaa agttagattt tctttcgagg
     2581 aattaaaaga gttaagtgat tataagatga ctgctacaaa agcctttgtc ggtgatttag
     2641 aaaagctcta taaagatatg cttaatctta cttaccgtac tgaagatgag gattctattc
     2701 gctactttgt tttgtttact ggttttgaaa tcaataaaac aaaaaagttt gttgaagtca
     2761 gtgttaatcc ggacctagaa tatattatta atggtttaac agctgaattt agtagatttg
     2821 aattatctgc tttcacgtcg ttgcgttcta cgtatgcaaa aactctattc cgactgctta
     2881 tgcagtaccg ttctactggt tattatgtag ttcgtattga agagtttagg agcttaatgg
     2941 atatcccaga atactatcaa atgggtaaca ttgatcaaaa agtattaaat cctgcattac
     3001 gagaact
//
LOCUS       DACCCS010000145         2918 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.156, whole genome shotgun sequence.
ACCESSION   DACCCS010000145 DACCCS010000000
VERSION     DACCCS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.156"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            204..1172
                     /locus_tag="IVK82_002857"
     CDS             204..1172
                     /locus_tag="IVK82_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP6393856.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1165..2196
                     /gene="vanA"
                     /locus_tag="IVK82_002858"
     CDS             1165..2196
                     /gene="vanA"
                     /locus_tag="IVK82_002858"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP6393857.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2202..2810
                     /locus_tag="IVK82_002859"
     CDS             2202..2810
                     /locus_tag="IVK82_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP6393858.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          863 a          559 c          709 g          787 t
ORIGIN      
        1 aattttttag gaaaatctca aggttatctt tactttttct taggaaatta acaatttaat
       61 attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga gccgttttcg
      121 ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt tggtgccttt
      181 cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat ggatgtgagc
      241 aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg gcaacgataa
      301 ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg
      361 tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga gccggtgtga
      421 aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct gctaagagaa
      481 tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat tatactatga
      541 tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg
      601 atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt ggtgtggtgg
      661 gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga tgtaaagtgt
      721 tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat gagttgctgc
      781 aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac tatattatca
      841 gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact gggcgcggtc
      901 cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg ggcggtgccg
      961 cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc caaaaaccaa
     1021 ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc acaccgcata
     1081 cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt
     1141 tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact gtttgggggt
     1201 tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc taacattaat
     1261 aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg gaaaatgtgc
     1321 gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact ctcgccggat
     1381 aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat
     1441 gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca aggtctgttt
     1501 gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat ttgtatggac
     1561 aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc cttttgggtt
     1621 attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt tgttaagccg
     1681 gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga attggactac
     1741 gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc
     1801 tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagctgttgg cgaggtggac
     1861 caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc
     1921 tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg aggacggata
     1981 caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc ccgtgtggat
     2041 atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct gcccggtttc
     2101 acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact tcccgaactg
     2161 attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata ggatttactt
     2221 ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact tgggataatt
     2281 tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca tacgagttgg
     2341 ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga ttgcttctat
     2401 gggacggtta ccgtcctaat cgtgctgtaa actgttttat gcaatgggct gcacagccgg
     2461 aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag atgatttcaa
     2521 aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat cttacgcttt
     2581 atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt atggatgaac
     2641 gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc
     2701 gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg cactatgtat
     2761 taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa acttttaacc
     2821 gttgcacgga caaactatat aagctaactc tttcggcagg aaacccgacg tatgtaactg
     2881 gttcttaggg aatttatata tagtagatag tattgaag
//
LOCUS       DACCCS010000146         2867 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.157, whole genome shotgun sequence.
ACCESSION   DACCCS010000146 DACCCS010000000
VERSION     DACCCS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.157"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..1127)
                     /locus_tag="IVK82_002860"
     CDS             complement(<1..1127)
                     /locus_tag="IVK82_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP6393859.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRD"
     gene            complement(1156..1542)
                     /locus_tag="IVK82_002861"
     CDS             complement(1156..1542)
                     /locus_tag="IVK82_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6393860.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(1558..1872)
                     /locus_tag="IVK82_002862"
     CDS             complement(1558..1872)
                     /locus_tag="IVK82_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6393861.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(2225..2704)
                     /gene="rlmH"
                     /locus_tag="IVK82_002863"
     CDS             complement(2225..2704)
                     /gene="rlmH"
                     /locus_tag="IVK82_002863"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP6393862.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT          764 a          594 c          502 g         1007 t
ORIGIN      
        1 tgatcacgga ttccgtcgcc taaatatttt gcgtctggac gttgacaagc cagtattaga
       61 aagaagccag cttgacgacc taacatgaca atctgtttca gcttattcat aactgcggtg
      121 ttttcttttg ttcccagcat ttccatgaaa gcgacgtatt catcaaagat taagaagtgt
      181 gccgggagac ctaagtaagc ataatttttg ccagtcttat agttcttcat ctgcttcatt
      241 tcctcactac gtttcatcat ttcttcatag aatgtttcaa tgcaagaaag caagtcttct
      301 tttctatagt agacatttgc catcacagaa cctaagtccg caaggtcagc atttttcggg
      361 tcaagaatat acagttttga atctgtatga agcaaggctt caatcagtgt cagtataaag
      421 taagttttac cgccacctgt accaccagca atcaacatat gagggagctt atcatattcc
      481 caccatacgt ttttcattaa gcgaagttta ccatctttag cttctacttc atcaatagaa
      541 atacgactgg ctatggtgtc atagagcaaa gtatattcca cataggaatc ctttaactct
      601 ttatccgtca gctcacagta caagccactc tctaatttct tttccaagtg taagagttgg
      661 tcttgatatt ttcccagcgt gatttccacc cgtatctgta tcaagccatt tttaagtcga
      721 taatacattt tagggaagta ggttatcttt tcctttgtac gaccagcact atctttaaag
      781 aaaccctctg ttttgacctg ttcagattca taccacttgt tttcaagtat catctttgcc
      841 agtttttgac ggtggtaaag ttgtttaacc gtatcatagc gaacccgttt gaatacaaac
      901 gctaccagca agcagataag aattgcgaca ctgaaactga taattaaata gggaatgtca
      961 atcttatctg cttgtgatag gttaaaatcc tgccagttga tctgctggat tgtcttcaca
     1021 tgaaacagtc cgacaaccag caggaaaaca ggcaggagtg acgctatcgt aaaatgaaag
     1081 actaaatctt taccagatgg gcgaatcctt ttaccacgct gtttcatgcg aaaaagtctc
     1141 ctttctacct agcgactatt tgtcttgtgt cggttctttc tttgcttgtg gttgagcttt
     1201 gaatgaacta gaatcctttg tcagcacaat atcgtctgcc ttgatatacc agtcaacatc
     1261 tgctccttga taggtggcag tagcaacggt gtccgcaatg ggattgataa gttccacccg
     1321 tgcgttataa tcaaactctt tcaaaggcac gctggcagga atacttactt gaatcatgcg
     1381 tccttgtcct ttggatttta agtcataggt acgttccttg atttcatctg aaaccgaccc
     1441 gtcttcattt tggattctca cttcacgacg tagagcagag aatttcaatt ctccaaaagt
     1501 cgtgtcttta tctaatacaa tgccatttgc taatctcatc atttttcctc tctttcttta
     1561 ttcttttatc atgtcgtcag catgtaaaag gtaatttgta aaaccacgag tgccgatttt
     1621 gtagccctct gcggtaatac gtggattgac taacttcaca cgttcctcaa agccgaaatg
     1681 tttttcgcca gcttcagcag gaagcaccac cacaatatca tctgctcttt gaacatcaga
     1741 atagagatta tagcttcttg ataagacagt tagccgtccg ttgattcttc gctgaacgac
     1801 tttatcctcg ccagcaaatt ctaaattgcc gaatgttttt tccatgttgg gaatcacaaa
     1861 tttaagttcc atatttttac ctatcctttc ttttttattg gctgaatgaa tgtttgatgg
     1921 tcttaaagag tggggaacga ccttttgatt cttgattttt tgttttcata agttcacttc
     1981 ctttcaaaat cgggtaaaaa aatagacacc tcattttttg aagtgtctac ctattaaata
     2041 ttcaaatttt attggaagta tctttatatc ttcacttttc aaggataaat cgtcgtatca
     2101 aagctcattc ataagtagta aattagtagt aaattgagtg gttttgacct tgataaagtg
     2161 tgataagtcc agtttttatg cggataacta gatttttatg ctatttttct cttaaaaaat
     2221 atcattattt gtggtatgga gcccccgcgt tgatccggaa ggcacggtag atttgctcga
     2281 cgaggatcag gcgcatcaat tgatgtggat acgtcatttt gccaaaagag atctgagcat
     2341 tgcttctttt catgacggct tcgctgagtc ctaaagaacc gccgataaca aaaacaaggt
     2401 ggctttttcc ttggatacct aattggtcga tttgtttagc aaaagattcg cttgtgggat
     2461 ttttcccttc aattgctaag ccatagacgt attcttgttc tttgattttc gctaagatcc
     2521 gttctccttc tttttctttt acttgtctca tttgcgcttc gcttaaattc tccggggctt
     2581 tttcgtcagg aacttcgatc aattcgattt tcgcataggc attcagccgt ttgacatact
     2641 cattgatacc ttggatcaag tatttttctt ttaattttcc tactgaaata attttgatat
     2701 tcatgttttc tctcctttga tcttcacgct cactttgttt attataatca attttttcgt
     2761 ctatatgttt attttgacta tttttgttaa aaagatttcc ccaacagtta tgcacaacat
     2821 ccacaggttt atccacagaa aacagcgttg ttttacctta tttcatc
//
LOCUS       DACCCS010000147         2695 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.158, whole genome shotgun sequence.
ACCESSION   DACCCS010000147 DACCCS010000000
VERSION     DACCCS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.158"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(25..243)
                     /locus_tag="IVK82_002864"
                     /pseudo
     CDS             complement(25..243)
                     /locus_tag="IVK82_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            484..666
                     /locus_tag="IVK82_002865"
     CDS             484..666
                     /locus_tag="IVK82_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393863.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            650..835
                     /locus_tag="IVK82_002866"
     CDS             650..835
                     /locus_tag="IVK82_002866"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP6393864.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            1078..2598
                     /locus_tag="IVK82_002867"
     CDS             1078..2598
                     /locus_tag="IVK82_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP6393865.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
BASE COUNT          902 a          463 c          541 g          789 t
ORIGIN      
        1 gttcttccct actgtagatt gtttctaatt ctccatcggt tgactcagca tttgttcgag
       61 ctcgccttcg gcctgttgga aacctttatg actgcgacct aggaattttt ggttgtaagt
      121 atcaaaagac gagactaaga aacgttccat cgactcttca ttttggaact gttccttgcg
      181 gtggctgtat ttcttgattt gtttattgaa agattcgatt aaattagttg agtaaatgct
      241 tcgtacgaac atagttgatt tattcaccat acttcccatt acatcgtaaa acgctatatt
      301 taagatttca aacaactata ttaataggaa gaaaacttca tttttcctct tagttctata
      361 aaaagtgtgg ctaatttgag gcaaaatatt aaattaaatg taagcgtgtc ataggatagt
      421 atctcgtacg ccgattatct ttcttctata attaaactat caaatataga aggagaaaaa
      481 cgaatgaata aaaaagatat tgtaccagtt caattttcaa gtgcggctca aaaatcgcaa
      541 attgacacca agccaaaact agcttgcaga atcaaatcgg aagataagga aatatttatt
      601 tataatggaa tggataaata tcttttgtat accttgttaa aagagttgaa tgccaatgct
      661 cattgatgca actgcattga aacatatttt tatcgtttgt ggaaaaacag atcttcgaaa
      721 aggaattgat ggactagcag ctttcgttat agaagaatat gaccttgatg tttacgatca
      781 agcgcttttc ttattttgcg gtacgaagaa agatcgcttc aaggctcttt actaggatca
      841 aaatggattt gtattaatgt acaaaagatt tgaaagtggg taccttcaat ggccgcgttc
      901 cagaaaagaa gtcttactga ctggcctttc aatcgaagaa aaatgtacgc taaaagaaac
      961 gaaaaaaggt gcgatatatt agacgaaaac cttgttaaat caaggatttt gtctgttttt
     1021 cgttgtcata aaatggtata atagatgtaa gaaaatcgct agaatggtgg tgtaaaaatg
     1081 acaaaaatgg aatctgaact tcttgtacaa atcaagcaat taacacagtt gcttgaaatc
     1141 cagcaacagg aaaatactct tcttagagaa caaattgctg aaatgaatcg acgcttgttt
     1201 ggacgaaaaa aagaaacacc acccgtagat gggcaaatcg atttattaga cgactcaact
     1261 tttaatgaac cagagcacac tggacaagaa agccaggaac ccattacggt atcttctttt
     1321 aaacgcagaa aacgcaaggg actcaaagca ctttcattag aaggtctgcc tgaagttcct
     1381 attcattatg aattaaaagg tgccgattgt ttgtgtgaag actgtggtca tactttacat
     1441 gatatcggtg cgacaaggct tagagaagag ccattgtatg ttcctgccca tatagaaaaa
     1501 caagtcatct accaacatgc ctatcattgc agagaatgtg aagcattagg agaaacaaaa
     1561 atcaaaaaag cgcctgttcc taaaccgttg atcaataata gtctaggttc ctcttcaatt
     1621 gtgacacaga gtatttggga aaaatttgtt aaaaaggtgc cggcatatcg acaagaaaaa
     1681 gaatggcatg cacttggttt ccctcttgat cgtaaaaaaa tcactaattg gcatatcaag
     1741 gtaagtgaaa tggttttaga gccaatctac aattgccttc atcaagaatt aagcaatcag
     1801 gaggtcttac acgcggatga gacaagctat cgggtattga atactcataa agaaaagacc
     1861 ttttactggt tatatgcttc aggtaaaagc gaagaaaaac aagttgtgct ctacgagcac
     1921 gcaaattcgc gttccgttga tgagccaaaa agttttttag aatcatttag cggtttcttg
     1981 catactgatg gttacgcggc ctatggaaag ctgccggtaa acaatgttta ttgttgggcg
     2041 catgttcgca gaaagttttt tgaagcattg ggtaaacaac cgtccccaaa aagtcatgcc
     2101 aaaataggat tagacttctg tgacaagatg tttcgaatcg aacggacctt taataagctt
     2161 agcgcagaag aaaggtttgt agcgaggaag gaaacgttgc agcctgtgat gactgaattt
     2221 tttcattggt gtgagagttt tgcttatttg ccaaaatcgc agcttggaaa ggcggttcaa
     2281 tacgcagtca atcataaaga cggacttcag attgtgttgc ttgatggacg cttggaactt
     2341 tcaaataacc gagcagagcg tgccatcaaa gagttagtga tcggtcgaaa aaactggcta
     2401 ttttctaaaa gcttaaaagg cgctagatca aatggaatta tattgagtat tatccagaca
     2461 gctgttgcga atgggttaaa catccgcaaa tatttaaatc atctttttac agagattccc
     2521 aatctgtctt cgatgacacc tgaagcgcta cgcgcttatt tgccatggaa tcagcagata
     2581 caagaaatct gtaaataata taagccacaa ataacctgat ttgagtgtat caaatcgggc
     2641 tatttgtgtt taggtaccat tctatgacac gcttacgctc aaaactactg aatta
//
LOCUS       DACCCS010000148         2674 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.159, whole genome shotgun sequence.
ACCESSION   DACCCS010000148 DACCCS010000000
VERSION     DACCCS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.159"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..135
                     /locus_tag="IVK82_002868"
     CDS             <1..135
                     /locus_tag="IVK82_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP6393866.1"
                     /translation="LETPYVGKDKKKAYAPYGKEISMFKAQAFDPEVFPELLAEEVTL
                     "
     gene            346..2121
                     /locus_tag="IVK82_002869"
     CDS             346..2121
                     /locus_tag="IVK82_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP6393867.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(2187..>2674)
                     /locus_tag="IVK82_002870"
     CDS             complement(2187..>2674)
                     /locus_tag="IVK82_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP6393868.1"
                     /translation="MRIPIWLRRLVTRLLSVIPVLICVLFTSTKGTIEEHAALNNLMN
                     ESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKLLGWFSVISLTYLNLRGLPG
                     QIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGDLYKGNKKYEQLLQETVEEI
                     E"
BASE COUNT          843 a          507 c          504 g          820 t
ORIGIN      
        1 ttagaaaccc catatgtcgg taaagataaa aagaaagctt acgcaccata tggaaaagaa
       61 atttccatgt tcaaagcgca agcattcgat cccgaagtgt tccctgagtt attagcagaa
      121 gaagtcacac tttaagtttt ctacctgaca gcaacaaaaa aggattgggc agtctgcctt
      181 gtcctttttt gtttaagaaa caaaggagtg ctttttgcat tcgttttcgc catgctcctt
      241 ttgccccctc tttttaaatt cagacaaaca gggtatactg atagaaaagt aaaaaacgaa
      301 aagtttcaca aacaagtagc aaaacgtgat tgaaaggacg aaaagatgct gaacatttta
      361 aaccccttga tcgaccaact aaaaaagaaa catacatact ttattcttgg catagccgta
      421 ttatttacaa ttattcggtt gtatttatat agtaaagcaa tctatggaac agatatgtcg
      481 gcgacctatg atgatcagct gctgattcac tatgctcaaa atattttgga tggtaaatgg
      541 ctaggggagt atacgactac aacactttcc aaaggaatct cctacagctt atttttagta
      601 cttgcacata aactgcatct ttcttatgca gttttgttcg gcatgttcag tatttttgcc
      661 agtatattgg ttgcactcgc gtttaaaccg attgtgaaga acaagtatct tcttttaagt
      721 atctattttt attttttgtt ttctccgatt agttttacac atgaatattc cactcgaaca
      781 tatcgtaatg ccctcgtgat tccggctgtt gagatcatta tcgcttgttt attagctatt
      841 tattaccgca aatacggttc tagcagaaaa ttgctgccat ggtttttagg gctttctgtc
      901 gcttttcctt tcttttggtt cattcgcgaa gattctatct ggcttcttcc ttttactgta
      961 gtagctttgg gtatcagttt cgtccagatc ttgctagggc aagacttttc tttcaaagaa
     1021 gtaaagaagt taaaaaaacg actctttctt ctctcgaaac gggaatggct gaaactttca
     1081 ttgtttttac ttcctatagt tggcttttcc atcgtctatc agtggatcga atataaaaat
     1141 gagacaacat acggtattgc aaccatcaat gaccgttcca gtggagaatt tggacgtttg
     1201 atgaaacaac tgatccgaat agatgatgaa acgaacttga ataaagaaaa cagtgagata
     1261 tgggtctcac atgctgcact aaataaagca ttagccgcat ctcctacatt ccatactgtt
     1321 tctacccgta ttgacgaatt atatacgact cacgcgtgga ctcaaggtgg aaaagtaaaa
     1381 gaattacccg gagatatcat cttttgggca ttgcgagatg tggtaagcga atctggttat
     1441 taccaaaaca atgcaaaaga aaccaatgat ttttggaaaa aagtcaacca agaattagaa
     1501 gaagcctatc acaagggaac catcaaaaag aaaaaagagt tctatctgat gggatcagga
     1561 gatggaaaga cgatgagtga tctgccaatc gtattcgatt tccttcaatc aggatacgct
     1621 tatgcatggc gatataaaga tttccatcaa ggaggaaatt atagtttcgg aacgcaagaa
     1681 caagtcaaac aggctcaaga attattaaac cttccgctaa tggagaattg gatcgatagt
     1741 aatgaaccga gaacacctaa tttccaatta acaaaatcag caaaagtcgc gaatgtcatc
     1801 ataaaaattt accaagttct ggctattccc gtgctgattg cttcaatagc agggatatta
     1861 cttatgatac taggaaccgt atttgcaaaa gctgatcgtg cctattatag ttcctctttg
     1921 attgtggtca ccggattaat actgacacaa ttggtttttc ttttcggtgt ctcatggttt
     1981 tgttcttatg cgccagaaaa aaaggactat tttttaagtg tctacactgg tgcaggcgtg
     2041 ccattaacgc aattcatcgt agtaacaagc atgctagttt tcagtaaatt aaatctgaaa
     2101 aaagctttat cttctaaata aaaagaccct tggtagctag atttttaacc taactatcaa
     2161 gggtctttgt gtggaaaaat cagtttttat tcgatttctt ctaccgtttc ctgcaaaagc
     2221 tgttcatatt ttttgtttcc tttatataaa tcaccaatcg tccatgccaa caggagaagg
     2281 acaagaacaa tgattacata agcgatgtga tcagccaatg ctagttgagc ggaagtggct
     2341 tgctcgccga agaaactttc gatctgtcct ggtaatcctc gtaagttcaa gtaagttaga
     2401 ctaatcacag agaaccagcc aagaagtttt acgatgaatg aatttttaaa tcggctaccc
     2461 atctccgctt ggctgttggt catcatcagt aaaggaatca tcgaaaatgg caacgcaaaa
     2521 gccaagaaca cctgggattc attcattaag ttattcaaag cggcatgttc ttcgatcgtt
     2581 ccttttgtac ttgtaaacaa aacacaaatc aatacaggaa tcacggaaag caatcgtgtg
     2641 accagtcttc ttagccaaat cgggatacgc atat
//
LOCUS       DACCCS010000149         2512 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.160, whole genome shotgun sequence.
ACCESSION   DACCCS010000149 DACCCS010000000
VERSION     DACCCS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.160"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(492..1784)
                     /locus_tag="IVK82_002871"
     CDS             complement(492..1784)
                     /locus_tag="IVK82_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6393869.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2058..2318)
                     /locus_tag="IVK82_002872"
     CDS             complement(2058..2318)
                     /locus_tag="IVK82_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393870.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          847 a          462 c          379 g          824 t
ORIGIN      
        1 tatattataa attcacatga ttatcgaatt ttattaagtt agtttttctt gtaaatctct
       61 gttcccaatg aaactcgaga cacttatttt aaagaggaaa caatgtggta tttaaatagc
      121 cctcttgtat gggctactct gtgatactta ttagccatta aactatttta cccacatagc
      181 atatattttg aaatcgttca ttccgtaaat tcaactaaat atgcctaaaa aaataaaaaa
      241 tctaagatag aataagattt attcagtctt agatggattt atagcaactt tttcgtaaca
      301 actttaaaaa atacgaaaag aaagagctgt tttactctga ctacgttgct aaattgtcgt
      361 ttaatcattc atttaaacca aatctttttt tatagaattc gttttaatcc tcctgaatcc
      421 aaaaactatg aaaaccgtaa ttaaatagtt aagagttttt aaccaccata attctctcgg
      481 ctattttgaa atcagataaa ttttgatcgg acacatagtt gaataaacct accgttgtta
      541 cggaaggtaa atcgcatact tttcattcta gaggagaagg tctcgttgat cattaactga
      601 ttaagcacca tagcccagtt ttgaattcga ccacctgacc acttcgcgtg taattctttc
      661 gtgcgtaaat aaagtagttt taggagtgca ttctcattag agaacgctcc tttttttgtg
      721 acttttctga agctggagtg gacactttca accgcattgg tagtgtacat aatttttcga
      781 atggcactac cataatcaaa tagttgttca acatgtgcaa agttccgttt ccagacatct
      841 acagcaccag aataatgaga ccaccgattt tgaaaactgt caaaagcagc atgtgcagcg
      901 tttagagaag aagcaccgta gaactttttc atatctcggc agacttcctt atagtcctta
      961 cttggaatat agcgcaatgc atttcgaaca agatgaacaa tacagcgctg aacaattacc
     1021 gatggaaaga tcgcttttgc gccttcttcg aggccagaaa caccgtccat cgaaatgaaa
     1081 aagacatctt cgacaccacg tgctttcagt tcatcaaata cttgcatcca gcgattttta
     1141 gattctgttt gatttaacca taatcctaaa atctccttat ttcctttgag atcatagcca
     1201 agaatggtgt atacagcata ttctttggct tcataatttt ctcgtaaagt aacatacata
     1261 caatcaacga atagaaaggc ataacacttt gctagggggc gggcttgcca ttcttccaat
     1321 tcaggaagga cagcgtcagt gatatctgaa atcatttcat gggaaatatc aaagccataa
     1381 atagcttcga cggttgcggc aatatctcgt tgactcattc ctcgtgcata catggaaaga
     1441 accttccctt cgatgtcgga gacatctcgt tttctcttag gaattaactc tggttcaaag
     1501 gaagcttccc ggtctctagg aacatcaata gctacttcac caaaactggt tttaagcgtt
     1561 ttagttccat agccatttcg acggttatcg tgttccttag gctctttaga atgggcatca
     1621 taacctaaat gattattcaa ttctccttga agcatttttt caaaaagggg cccaaacaca
     1681 tctttcaaag catcttgcat gtcatcgaca gattcaggtt gataggcatt cagaatggat
     1741 tcagctaact tttcggcatc aggatttctt ttctttctag ccatcgtgta atcacctctt
     1801 gatcttattg tagaaaaaga aaaaccgcga agcaaccacc tgcctaggat taatggttac
     1861 ttacacggtt tatattacac tctcatcatt aaaaaagtat ctttcgatat tactaaacaa
     1921 tgaaattatc tatttgaaat ttaaatctga taatatattg agtgttccaa tgagggcttt
     1981 gatcctatta tccatatctt tttcagtttt aatattgata cacattataa gaaaaagaaa
     2041 cttttggtta atttatttca tcccagtgca aaatataaaa aagtaaaata gaccataggc
     2101 aatagattaa caattaatat aatacaagca aatattctat tcattgtatt tctgctttca
     2161 aaaagttcta atattgaaag tataattcca caaatgccta taataagtat gggaataaga
     2221 ttagaaaata acaccccgag ccaatctgtg aaaattacta tatttactaa tatccctatt
     2281 aacataccta tcatatttat taaatcaatt tttttcatta gatatttctc ctttattaaa
     2341 tctgaagtac ttatattaaa ataattctaa actatttacc tcaaaaagtg caataaatta
     2401 tttctgaaca ataaaatgat agtcaaaaca aaaaaatatt gttaatcgtt cagagccatc
     2461 cgagagtgta aaatattttg tgtaaatgaa aaaatccata caaaaaagga ag
//
LOCUS       DACCCS010000150         2484 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.161, whole genome shotgun sequence.
ACCESSION   DACCCS010000150 DACCCS010000000
VERSION     DACCCS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.161"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(1015..1305)
                     /locus_tag="IVK82_002873"
     CDS             complement(1015..1305)
                     /locus_tag="IVK82_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393871.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1513..2204)
                     /locus_tag="IVK82_002874"
                     /pseudo
     CDS             complement(1513..2204)
                     /locus_tag="IVK82_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          459 c          348 g          943 t
ORIGIN      
        1 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
       61 gcataaagtg tttagaaaag aacattaaga atagttctat aataattctt gaactccatg
      121 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      181 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      241 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      301 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      361 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      421 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      481 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      541 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      601 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      661 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      721 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
      781 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
      841 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
      901 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
      961 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1021 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1081 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1141 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1201 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1261 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1321 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1381 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1441 actaaaaaaa agaggcagca gtatagatta ctgttgcttc tttactaatt taggtttact
     1501 tgttaagaaa gattatttac gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt
     1561 atcttccact tcatcttttg ctttttctaa cttttgaata tattgtttca agcttgagtt
     1621 ttcagcagtt gcttcgtcta atttcttttg tgtatctgtt aactgatttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2281 ttacatatgt aattttaata taaatactat caaatactca tttaatcctc cttttttatc
     2341 tatttaaaaa atctttttta tagagaaaaa tgcaggttaa tactttctta aataaatatt
     2401 tatggtgaac aaaaacaaaa aagcaagttt tattaaacgt aacccttttc atttcataga
     2461 ttactgtatt tattacaaaa aacg
//
LOCUS       DACCCS010000151         2455 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.162, whole genome shotgun sequence.
ACCESSION   DACCCS010000151 DACCCS010000000
VERSION     DACCCS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.162"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            195..467
                     /locus_tag="IVK82_002875"
     CDS             195..467
                     /locus_tag="IVK82_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393872.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            912..1091
                     /locus_tag="IVK82_002876"
     CDS             912..1091
                     /locus_tag="IVK82_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393873.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            1150..1506
                     /locus_tag="IVK82_002877"
     CDS             1150..1506
                     /locus_tag="IVK82_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393874.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT          893 a          338 c          467 g          757 t
ORIGIN      
        1 aattagagaa ctaaaaagga gaaaaaaata tggctcaaaa ttactatgac gaatttgtca
       61 aattaccttt ggataaaatg gcacaaaaaa tggaggatat gacgttccta tacaacgaaa
      121 cacgtgttcc aaaaaaacat tataaagaaa aattgtcggt agctgtggaa gaaatgattg
      181 aataaggtgt cgaaatgaat cttattgcga cgtactatcg aacactggaa gaactgaaaa
      241 agcaaaatgc aaaatggttt ttccaagcct tactatgctt ggaagtaggt gtcaaaccaa
      301 gcactattaa accttctgaa taccaagctt tagaactaac gtatgcgaaa tttatcgaaa
      361 cgaaaaaagc caagactgtt tcaagtgaat ggttagatta ttttgaaaat atcaataagt
      421 atggcgcata ttacaccatg aaaaaggaag acaacgaaaa tgaataaggt gatttatcaa
      481 ctaacaacgg attccatatc aacgatttga aagaaaaaaa ggtcaataat catttatacc
      541 gaaaagtgag aatgtgtgaa acaaggacaa aaaacgccta gtaactaagg aaatggttct
      601 tatttcgcac caatcgctcc aaccgccccc gccgcttagg tggtgcgaaa taaacgggca
      661 aagcccgata acttaagaga aagaaggtga tccatttgaa caagattatt cccttattgt
      721 tagtactgtt tgttggcgaa atacttatgc gagatagtgc agctgtacag ttaaacctcg
      781 ctgtattttt ggtaatactg gtgttttgtt tgatcttttg gcggtttgta ttagtcctcg
      841 tgagtctact attcaagatg attgcagcca tatttttcat agggttattt cgatgaaagg
      901 agtgagtacc aatgccaatt gcaataggaa ataaacggtt acccgtgaca ttagatgaaa
      961 agagacaaaa agaattgcag caactaaagc agaagtatgg caaaagtgaa tctaagatta
     1021 tgtgtattgc gttagatcta ttgatcgcgc aagaaaaagc aggatttgat gtaccagcgc
     1081 taaaaaagtg acgtcacttt ttaatttgat ctatataagt gatatcactt ttaccaagga
     1141 gggaattaaa tgttaaaaac taaaaagaat tcgtacaaat taaagcttag tatactatct
     1201 attatttgtc ttttaggcat gataattagt ggtcatttgt atagagtggc atatacgcag
     1261 tttattgagt acaaggttca ctctttagga ttagctgggg atcgatacgt tgaatatgtc
     1321 cattattcat taaaatgttc ttcatatatt cttctttggc ttattttttc attgtttgaa
     1381 ttgctaaatt taatagcaat atcttcatat cacttagata agctaaatac caaatctagc
     1441 cgttataacg aagtgtttaa ggaaaatgtg aagttagaaa gtgacttaaa caagctaaaa
     1501 aaataaaaag aagaaaacat aagttaacta aaattatttt attaagaatg atggaatata
     1561 aggataaggt taccaacata acgatcatta cttctctgat atgctatgga cttctatcaa
     1621 tcagtatttt gtctatagat tatggagtaa ctatactgca agccttagtt ttggctatgc
     1681 ctatgtatct atctttgttt atcatgcatg aaatatataa gctatttgtt ttatcaaaaa
     1741 atgtagaaat acgggtaaat attttggtga atagttatca aaaagaactc aaagaaagaa
     1801 gacggtggcg gtatttattg aaaggatgaa aaagtaggat ttgacatact ggtattcaga
     1861 aagtgccgtc acttttaaaa caatggaaaa ggtggagata agatgaagaa aaaacaaact
     1921 aaactatcat tagcagatat ttaacacagt taaccgcaac tgaagatggt gttgttgaat
     1981 ttgaacaatc tgaaattgcg gtagtagatg atcgttacct taaaatgcct tacttctttg
     2041 accaagcaaa ggtcatttgc ctttgtcact acgatggggt acgtgatttt aggcgttaaa
     2101 aagtagtgat tatatcaact ttacgagtaa atataaattt agacatgaaa ctgaattatc
     2161 caatggaaaa tcatggttta taatctagaa aaattatagt aatgattgga ttatatatat
     2221 tctttcgtat tttcgtacaa ctgtagcttg tcaaaattga gaaatttaac cgattaggta
     2281 ctttacatcg ttatttgggt aatttctaaa acatgaagct taccaacgaa taaactgaga
     2341 ctatcttatt aattgatcaa tataagaaaa tatctttgag gagatgatgt actatgtttt
     2401 cgtctatcca cgattggtta ggtggtcttt tcggcggtgg aggtaaaggt tttta
//
LOCUS       DACCCS010000152         2398 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.163, whole genome shotgun sequence.
ACCESSION   DACCCS010000152 DACCCS010000000
VERSION     DACCCS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.163"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            207..2069
                     /locus_tag="IVK82_002878"
     CDS             207..2069
                     /locus_tag="IVK82_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6393875.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="IVK82_002879"
     CDS             2069..>2398
                     /locus_tag="IVK82_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6393876.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DACCCS010000153         2368 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.164, whole genome shotgun sequence.
ACCESSION   DACCCS010000153 DACCCS010000000
VERSION     DACCCS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.164"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..1451)
                     /locus_tag="IVK82_002880"
     CDS             complement(<1..1451)
                     /locus_tag="IVK82_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HAP6393877.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFI"
     gene            complement(1454..2200)
                     /locus_tag="IVK82_002881"
     CDS             complement(1454..2200)
                     /locus_tag="IVK82_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6393878.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
BASE COUNT          608 a          551 c          407 g          802 t
ORIGIN      
        1 taaataaacg gcagatttaa tttattataa tgttcagcta ccgtttcatt ggccgcaagc
       61 atgaaagatt cgatcaagcg ttcaccaatt cctctgcttc ttagctggat atcatttggg
      121 tgtccttcag catcaacaag gatttttgct tcgcgatcct caaaagaaat cgcgccacgt
      181 ttgattcgca tcgtttcaag gattcgatgc aattcaccca tttcttggaa cataggtacg
      241 agctgagcat agcgttgcat cgtttcttcg tcttgatctt ctaagatttg attgacagct
      301 gtgtaggtca tccgttctgt tgtttggatc acacttggga aaatatcatg agagactact
      361 tgtccatttg gatcaatctc catttcgcaa ctcatggtca gccttggaac atggggattc
      421 agtgaacaga taccattcga taaacgttgc gggatcatcg gaatcactcg atcagtcaaa
      481 tatacacttg tccctctttc atatgcttct ctgtccaatt gactgccttg tgtcacatag
      541 taagaaacat ctgcaatgtg tactccaaga aaataattac cgttatctaa ttttttaacc
      601 gtaactgcat catctagatc ttttgcatct tctccatcaa tcgtaacaat gagctgattt
      661 cttaaatctc tacgtccagt tatatccttt tcgctgattt gatctggaac ttcatctgct
      721 tcagacaata catcatcagg aaatttcgta gggataccat tagccacgac aattgagagg
      781 atatccatgc cagggtcatt cttatgacca atcacttgct tgattatccc ttctaaacta
      841 gtcgcatacc ctttttcagg gtaatgggtc agttcgatca gcacaatact tccgtcaact
      901 ggacgaactc cctctgctgc tgcatatact ttaaattggt tgagctttct atctttcggg
      961 ataacaacgc cgtaaagatc cgtttcttgt atttcttctt ctgaataagc aacgaattcc
     1021 cctgctaact gagtagtcgc ccggtctcga atctcgacta ctttcccctc tgctccgcga
     1081 tccatatctg tatttgctcg cttcatgata tcgattgcta cggtgtctcc atccattgca
     1141 aaaccagtcg cttcttttgg gatatagatg tcatcttctt cgggatcaat cgttacaaat
     1201 ccaaatcctc gttcatttgc tcgaaatgtt ccttcgatca agattggttt taaaggaagt
     1261 ttcacttttc cttttttatt gaaaactata ctctgttctc tttccatttg agcaatcgtt
     1321 tgtactagta atttaaaatc atcgcttttt tggaacccta aaccttcagc gatttcttcc
     1381 atagaaaaac tgcgtttgcg actgctttcc atgaaaaaga gaattttctc ttttatcgtt
     1441 tcttttgtca tatttattcc tcattccaag gtaatttttc taaaaattcc aagacgtctt
     1501 tttctaattc gcgtcttgct gtaccaactg taatcacatg accactttca ggataccatc
     1561 gaagtgtcac tcgctgacgg ctcaacttgc gtgcagtttc aaatacgcca ttagggtcga
     1621 tcatttcatc tttcccagct tgagctaaaa atatcggctg ttggatatct gacaacctac
     1681 tttctacgat tgctgcctga tcttgtatat ccattaattg ttgttcagct aacgagcggt
     1741 aagcaactgc tttttcattg atttctttct ctgatttacc tgctgtcttc agcacttgac
     1801 gtacgtattt ttcaaagttt tcaggcacgt ttgtttttac aggagacaaa ggcgaacaga
     1861 aaaaaccgcc gccaatcact gattcttctt ctagtgctcg tacagcaaag atgccgccca
     1921 ttgataatcc taatacggca atatcagaaa actgctcaga ctgtaaaaaa tggatggctt
     1981 ttttagtgtc tgcccaccag ctttctgctt tttcttgcaa aatatcatat ggctccattg
     2041 ttccgtgtcc tttaaaaagc ggcgcataaa ctgtataatc tgctttttcc aaaaatcgtg
     2101 cgagcattcg aacatcgttt ggacttcctg agtaagcgtg aagcagcaaa accgctcgct
     2161 ttccatgttt tgcataaagg ggttttggta attgtctcac tcgtctcacc tctttcgcct
     2221 cattttacca tagtttcttt cataacttct tcttaagcgt tctctttgca ataaaaaaag
     2281 ccgcccgccg ttttgcgaga ggcttattca actacttcga agataaatat gctaggacaa
     2341 ataataagat catccagata gctcccat
//
LOCUS       DACCCS010000154         2310 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.165, whole genome shotgun sequence.
ACCESSION   DACCCS010000154 DACCCS010000000
VERSION     DACCCS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.165"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            1194..1748
                     /locus_tag="IVK82_002882"
     CDS             1194..1748
                     /locus_tag="IVK82_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6393879.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            2002..>2310
                     /locus_tag="IVK82_002883"
     CDS             2002..>2310
                     /locus_tag="IVK82_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393880.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT          901 a          254 c          356 g          799 t
ORIGIN      
        1 ataaaaatat agaactaaat aggaaaacgt agacgtaatt ttctacgttt tccttttcat
       61 atatattaaa tattacggaa atttatataa actaatttag tgttttgttt gtaattccta
      121 attttgctcc acttttagat aatttttagg tctatttaac tcatcagaca ttctatgctc
      181 attttctcca aatttgtttc aactctttcg tacttaattt tgcgtatttg aaaattcttc
      241 ttttagagta cttttattac gatactagta tgatatatat ctatagcaaa cagttaaaat
      301 cttcctaaaa taagcgaata gcattcctaa aacctataaa atttttagat gaagcgacaa
      361 gtggacaatt aataaactac ttgaacatga aattatggat cacctttcaa atttagaaaa
      421 tggaattctt atacttattg cgcaacattt attgattgca aaattatgtg acaaaaagaa
      481 ctagtagaag aaggaataat ggcttgataa taaagtacta cctattaaag gtagtacttt
      541 atttaattta gtataaatta acatactact atattttaag tttcatatta tataaattaa
      601 taatatacta ccattttttg gtagtataat ttaaaggtaa taagttattc ttttattact
      661 atgtttgggt tttatagcaa ggaaagttta ataagagttt ggacctagca aacaatatta
      721 atgaggtcat taaatcttat tatctttagt gatataacga aatatctaca cagacaaaat
      781 tatgatcttg cgtaaagtta taaaatatta ataaaaatat tttattaatg aaaagcttgg
      841 tagcacggtt attcatatgc taatttattt cactttatag taaggggagt tcttagtata
      901 ttgtccaaat ttaaatttca tataatttaa ttatgtaaat tagctattct aagaacttct
      961 acggataact gcccgtctaa aatttaatta agagcttaga atcttcatat aagtaaggtt
     1021 aatttttttg tacaaaatag tcggaatatt ctgagttact gaaatgatat aaaatcataa
     1081 actaaacccc ttgattttct tcacttttaa cagagtatat atataagttc atgattatgg
     1141 taaaatatat atataatcat gaactcagaa tatttttaag aagggaaaca atagtgagct
     1201 ataatgtaca accgttaagg acaaatgaag agatacaaga ttttcagttt tggctaagaa
     1261 gaacaagtaa tccagaaaga gatagttttt tatttttgtt tggaatcaat aatgggttac
     1321 gtatgtcaga tattatagga ttaaaagttg gagacatacg aggtaaaagt aagcctatta
     1381 ttgttgaaca taaaacaggt aagcgaaaac cgatttttat agataatttg agagaagaga
     1441 ttttattata tactgaaggt aaagaagaaa atgattggtt atttccaagt cggcaacaag
     1501 gtcgacatat aacaagggat cgtgtctatc agatatatgc agatatagca gaaaaattag
     1561 gaagagatga tataggaaca catacattaa gaaaaacttt tgggtatcac tactacaaga
     1621 aaactagaga cattgctacc ttaatgttta tttttaatca tagctcgcaa gcaattacaa
     1681 aaaggtacat tggtataact gaagatgaaa taggggcatc gttacgtggt tttaaattgg
     1741 gaggctaatt gagatattag gatatatttt ttgtgtataa tgaaaactta gaaggtgaga
     1801 ttaatgaaga taactgaatt ttcggaagta tttaaaacgt ttagtaacat ttacaataga
     1861 ataccagaga tagaacaaaa tgttgtagtt gataatgatt attatataga gaatgatttt
     1921 aataaaccaa gaaaatatga caacctatac aattttatac acaatagaac aacaataaat
     1981 aacaacagta acactcgagt tttgattaat tcagcatatt ttcatattaa atatttaaag
     2041 aaatatgagt atgaagaagt tgtattagat tatgggctaa atagacagtc atttttaatt
     2101 tatgcaataa ataacggaaa tttgccaagc aataaaaaga acataattat tgaaacatat
     2161 gcctatctgg aggggggatc taaaaaacta attaagtcat gtaatataga gtatcaatta
     2221 tcagtaggag aaattaaatg tgttttcgaa gaagatttag aacttgactt aaaaaaaatt
     2281 tttgccagca ttcatgatta tataggtttg
//
LOCUS       DACCCS010000155         2269 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.166, whole genome shotgun sequence.
ACCESSION   DACCCS010000155 DACCCS010000000
VERSION     DACCCS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.166"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1355
                     /locus_tag="IVK82_002884"
     CDS             <1..1355
                     /locus_tag="IVK82_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP6393881.1"
                     /translation="YGFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSG
                     YIQEFEWVEEVTLAVAEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEED
                     IYVSFETPNSEEIQQEVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVF
                     FGMMVADVGYGLLMLIGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQ
                     ALPKELFGIRLPFPILSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAV
                     SDGFAWQGILIGIVIALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGA
                     AKGAYNLYGLTGYIGDLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLII
                     ALHALNMFLTLLSAYVHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK
                     "
     gene            1374..1844
                     /locus_tag="IVK82_002885"
     CDS             1374..1844
                     /locus_tag="IVK82_002885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP6393882.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            1865..>2269
                     /locus_tag="IVK82_002886"
     CDS             1865..>2269
                     /locus_tag="IVK82_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP6393883.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTL"
BASE COUNT          724 a          398 c          503 g          644 t
ORIGIN      
        1 aatacgggtt caataaagaa acgtatcctt atgaacaaaa tcctaaagaa aaattgcaag
       61 aaaccaaaca gcaattatcc attgttcacg agcagcaaaa aaagataacg acagcgttag
      121 gtcagtgcag cggctatatc caagaatttg aatgggtaga agaagtcaca cttgccgttg
      181 cagaacggga aaaagtaaaa gaacggttta tccatgccac ttatctgatc gttttgcaag
      241 gttgggtaga tgcagaagaa aaacagaatt tgatgcatgt gttaaacgaa actgtatcag
      301 aagaagatat atacgttagt ttcgaaacac caaactcaga agagattcag caggaagtcc
      361 ctactaaatt gaaaaaccat ccgcttatcg aaccttttga attgctgact gaaatgtaca
      421 gtctgccaaa atatgaagaa gtagatccaa cgccttggat gacaccattt tatcttgtct
      481 tcttcgggat gatggtcgca gatgttggtt acggattgct gatgctcatt gggacaatac
      541 tagctcaaaa actatttgtt ttaccccgcg ggatgaaacg tttcgtaaaa ttctttgaga
      601 ttctctcggt accatcgatc atctggggat tgatctacag ttcatttttc ggacaggcat
      661 taccaaaaga actatttggt atccgcttgc ctttcccaat cctatctacg acggatgacg
      721 tcaatacgat tttgatttta tccgtgattt tcggtctgat acagatcttg gttggattat
      781 tcgtggcagc aaaggaaaat atcaggagga aagattactt gagtgctgtc agtgatggtt
      841 ttgcctggca agggatcctg attgggatcg ttattgcatt gattggtgtc atgttgataa
      901 aaaatacagc atttgtttat cttggcgcgg ggctatcgat cattgctgct ttatgtatct
      961 taatcgtgcc gattatccaa tcgccgtcta aagtcaaggg tgctgccaaa ggagcgtata
     1021 atctttacgg attgactggt tatatcggtg atttagtcag ttatacacgt ttgatggcac
     1081 taggtatttc aggcggaagt attggcgcgg cattcaatat gctagttgct ttcatgccgc
     1141 cagccgctcg attcagtgta gggatccttt tgatcatcgc tttacacgca ctgaatatgt
     1201 ttttgacact gttaagtgcc tatgtccatg gtgcacgatt gcagtatgtc gaattttttg
     1261 gaaaatttta tacaggaggc ggacgtgcct ttgacccgct gaaaacggcg gaaaaatacg
     1321 tcaacatcaa tcataagaaa aaaaacaata aataattgga ggatttttat acaatgatgg
     1381 attacttaat tagtcaaaat ggtggaatgg tatttgcagt attagcgatg gcaacagcga
     1441 cgattttttc agggattggt tcagctaaag gtgttggaat gacaggtgag gcagcagcag
     1501 cgttaacaac aagtcaaccc gaaaaattcg gacaggcttt gattctgcaa ttgcttccag
     1561 gtacgcaagg gttgtatgga ttcgttatcg cctttttgat ttttatcaac ttaggaaatg
     1621 atatgtcagt tgtacaagga ttgaactttc taggcgcttc tctgccgatt gcttttacag
     1681 gtttgttttc aggtatcgcc caagggaaag ttgcagcagc aggtatccaa attttagcga
     1741 aaaaacctga acatgctaca aaaggaatca tttttgctgc gatggttgaa acatatgcga
     1801 ttttaggttt cgttatttcc ttcctattag tattgaatgc ctaagcataa aggaggaaaa
     1861 gcagatgaac gccattgaaa aaatcatctc tcagatgaat gaagcagcag agcaagaaag
     1921 agcggcgtta gagcaagaag agcgaatgaa gatcgatcaa aactttgaac aaaaacgtac
     1981 gcaaatcgaa acagaacatc aaaaacaaaa agaaaaacaa atcgaattac ttgaaaaaaa
     2041 ataccgtcag cttaggaatc gccaacaagt agaagtacga caagaaaatt tgaatgcgaa
     2101 gcaggaattt ttacgacgat tgtttgcaga tgcaatagct gaaatggaga attgggatga
     2161 atctgaacaa atccagttta tcaaaaattc attgtattca ttacctttga caggcaaagt
     2221 aacattcatt gctggagaaa aatcagcagc ttatctttct cagacattg
//
LOCUS       DACCCS010000156         2189 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.167, whole genome shotgun sequence.
ACCESSION   DACCCS010000156 DACCCS010000000
VERSION     DACCCS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.167"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(325..528)
                     /locus_tag="IVK82_002887"
     CDS             complement(325..528)
                     /locus_tag="IVK82_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393884.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(987..1952)
                     /locus_tag="IVK82_002888"
     CDS             complement(987..1952)
                     /locus_tag="IVK82_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP6393885.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DACCCS010000157         2155 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.168, whole genome shotgun sequence.
ACCESSION   DACCCS010000157 DACCCS010000000
VERSION     DACCCS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.168"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            181..483
                     /locus_tag="IVK82_002889"
     CDS             181..483
                     /locus_tag="IVK82_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393886.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(999..1072)
                     /locus_tag="IVK82_002890"
     tRNA            complement(999..1072)
                     /locus_tag="IVK82_002890"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1036..1038),aa:Ile,seq:cat)
     gene            complement(1627..1941)
                     /locus_tag="IVK82_002891"
     CDS             complement(1627..1941)
                     /locus_tag="IVK82_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393887.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          794 a          325 c          333 g          703 t
ORIGIN      
        1 ttttataagg gtttgagcat tttttatttt cgcagatttt cgttaaatga atgaaaatgg
       61 cattcaaaat agcattcaaa aagaacactt tttaatatat atgttccatc catattatca
      121 atgatattcc tactttacaa aaagaacatt agttcgtata ctctttttga ggtgaacatt
      181 atgttgatgg aaggaaaaac acaattatgg tttaaatttg atccttcgaa tagatttgtc
      241 aaagattttt ataaggtgtg ggattcagaa gttttctttt tagcaatcga agatagctta
      301 ttaatcaatc tctattactc taataagaac tactttaaaa ttccggcaac aaaaaccaga
      361 atgaagaaag atgtgtattt tttgtttgat atcgtgactg atgtgccaga cgcacggagc
      421 gatcatcggc gttatgacta tttgaagtat actttcgttg atccagaaag gtataaagat
      481 taaagtaggc tacctaaaaa aggtagcccg gaacggattt tatcaccata cttataaaag
      541 ggatattttt taagttagta ttaagattgt gtaatatgat gatatctata ttttatagta
      601 tcagtgctat aaaatcaaaa ataagtcact aattaactac cactccaatt ataagtcttt
      661 ttcccattat tttttacagt tatatggtat gctttttaat ggcttcaaat ataaaagagt
      721 ttaaagcgta acacacttat ggggaagtgg tttttgggga acgctttaaa ctcttcttta
      781 ttattatctc acaatataac ccaaatgtct ttccatttaa aaatcaaagt aaaacttttc
      841 aaatatacag aagtataact atgtgaaaca tcctttcatt aatccataaa aggatacata
      901 aaaaagccac tcatttgagt ggcaatgaag aaaagcttta gcttgtataa tactcttcaa
      961 aaaattctaa cacagaacga ttcaattggc tacattaatg taccctgtag gactcgaacc
     1021 tacgaccgga cggttatgag ccgtctgctc tgaccaactg agctaagggt agtggttgtt
     1081 gccacataaa gccataaaca atcaaccagt agaatgcgtg gcaacaaacc tgttatcgca
     1141 tatcttggag tgtgactatt tatgagtgat agtgaagata tgcgtctcca tatatgtatg
     1201 gttgagacag ataaacatgt atgaaaaacg ttgttaaatc aacgtttttt tattttggtc
     1261 aaggagagaa aagtagagaa atacaaaatt ttttgcattg attttgcatt caaaaaaaca
     1321 ttcaaacatt tagaaatccc caatacgtat tcaggaaaat ctattttaag gtttacaaaa
     1381 agaacttttg ttcgtacact ctttttgagg tgaactttta tgctgatgga aggaaaaaca
     1441 caattatggt ttaagcatgt tccttcaaat aagtttttta attataaact ctagagaaat
     1501 taattcatcc taacataaaa taattgttca ctcaaaagtt tttctagaac aattaaaaca
     1561 gcaggcttca cattcgctat acatttgtaa gaagtaataa ttcctgtact aaaaggcata
     1621 attgctttaa ttattttttg acttctcttc aaacacaaga agtagttgtg taataattgc
     1681 agaaataagg attccaataa tagatataaa atttgacatg gaaacgtcta taacattaaa
     1741 agaataaagt gttgaagaaa gtaaactgat tgaaataacc cctccgtaaa aaatagtctg
     1801 tgaatatacc cacttttttt cagaagacgt acttcgtttg gttctaaaac ctatccctgc
     1861 atttctactc ttatcaaaaa accaaatatt tagaagtaat aatattgcta ttattaagat
     1921 aaaattactg taaaaatcca aaagctatcc gagagtataa aatattttgt gtaaatgaaa
     1981 aaatccatac aaaaaaggaa gtcccttctg tagaataaag ttaacgacaa ccaattcaca
     2041 gaaaagagga cttccctatg aatgatttta ctacagaaat tgtgcaaact ctagtcacta
     2101 aaggcgattt aaatgaatta ttccgttcgc acttagaaaa agcgataaac acact
//
LOCUS       DACCCS010000158         2143 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.169, whole genome shotgun sequence.
ACCESSION   DACCCS010000158 DACCCS010000000
VERSION     DACCCS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.169"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..329
                     /locus_tag="IVK82_002892"
     CDS             <1..329
                     /locus_tag="IVK82_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6393888.1"
                     /translation="SILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILAD
                     EPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNK
                     MNLEVE"
     gene            329..1705
                     /locus_tag="IVK82_002893"
     CDS             329..1705
                     /locus_tag="IVK82_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6393889.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            1835..>2143
                     /locus_tag="IVK82_002894"
     CDS             1835..>2143
                     /locus_tag="IVK82_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6393890.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     N"
BASE COUNT          833 a          262 c          392 g          656 t
ORIGIN      
        1 aaagtatctt gtttgaacta ggtttagata aaaaacaaat aaaaagaaat gttatgaaat
       61 tatctggtgg acagcaacaa agagtagcta ttgctagagc attggtatca gatgctccaa
      121 taatactagc tgatgagcct actggtaatc tagacagtgt tactgctgga gaaataatta
      181 atatattaaa gacattagct aaagatagaa ataaatgtgt aatagttgta acacatagta
      241 aggaagtagc agattctgcg gatatcattt tagaactaag tggtaaaaag ttgaaaaaag
      301 taaataaaat gaatttggag gttgaataat gataaaaaat gcatttgctt atgtaactag
      361 aaaaagttta aaatcattaa ttatcatatt agttatttta tctatgtcaa ctttaagtct
      421 cattagttta tctattaaag atgctacgga cagagcttca aaagaaacat ttgctaatat
      481 aacaaatagt ttttctatgg agataaatag acaagtaaat ccaggaacac ctagaggtgg
      541 gggaaatgta aaaggtgaag atattaaaaa gatatctcaa acagatagta tagactccta
      601 tgtaaaaaga ataaacagtg ttgcagattt agttgattat gatattattg aaactcaaga
      661 gactcttgcg aatcaatcac ctgaaagagc aaagaatttt aaaagaacag ttatgttaac
      721 tggggttaac gactcaacaa aagaaacaaa atttgtatca gaagcatata aattagtaga
      781 aggaaagcat ttagaaaatg aagataaaaa taaaatctta atgcataaag atttagctga
      841 aaaaaataac cttaaagttg gagataagat aaaaataaaa tctaatttat ttgatgctga
      901 taatgagaaa ggtgcagatg aaacagtaga agtagaaatt aaaggtctat tcgatgggca
      961 taatagcggt gtagtaagtg cagcacaaga actatacgaa aatacattaa tcactgatat
     1021 tcatacagct gctaaagttt atggtaatac cgaagataca gctgtatacc aagatgcaac
     1081 attctttgta aaaggtgata agaatcttga tagtgtaata aaagatcttg ggaaattaga
     1141 tataaattgg agagaatata atttgattaa aagttcatca aattaccctg cattacaaca
     1201 atctatatca ggtatttact caatttcaaa taaattattt tttggctcat taatatttgc
     1261 aggagttgta gtttcattat tattattctt atggatgaat gctagaaaga aagaaatagc
     1321 agtattacta tcattaggta tatcaaaatt agaaattttt ggtcaattcc taattgagat
     1381 ggtatttata tcaatccccg cattcgttgg ttcctatttc ttagctcaat atacagctga
     1441 caagcttggg aataatatat tgaataaggt tactggtgat atagctaaac aaatagctaa
     1501 acaatctgca tctagccaat taggtggtgg ggcagaagct gaaggattta acaagacatt
     1561 atcaagttta gatatcaatg tattacctaa attcatgatt tatgtagtta tatttatgag
     1621 tttagtactt cttgtatcat tgattatttc ttcattcaat atattaagaa ggaatccaaa
     1681 agaattatta attgacaata attaaaattt tcggtgcttt ttagcaccaa aaatgagagt
     1741 gtaatataaa ccgtgtaagt aaccatcaat cctaggcggg tggttgcttc acggttttta
     1801 tttttctaaa ataagatcaa gaggtgatta cacgatggct agaaagaaaa gaaatcctga
     1861 tgctgagaag ttagctgaat ccattttgaa tacttatcaa cccgaatctg tagaggatat
     1921 gcaagatgtt ttaaaagatg tgtttggtcc cttatttgaa aaaatgcttc aaggagaatt
     1981 gaataaccat ttagggtatg acgcccattc taaagagcca aagaaacatg acaatcgacg
     2041 aaacggttat ggcaataaga cgcttaaaac gagctttggt aaagtaaaca ttgatgttcc
     2101 cagagaccgg gaggcttctt ttgaacctga gttgattcct aat
//
LOCUS       DACCCS010000159         2070 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.170, whole genome shotgun sequence.
ACCESSION   DACCCS010000159 DACCCS010000000
VERSION     DACCCS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.170"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(146..1474)
                     /locus_tag="IVK82_002895"
     CDS             complement(146..1474)
                     /locus_tag="IVK82_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP6393891.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
BASE COUNT          635 a          403 c          339 g          693 t
ORIGIN      
        1 aaaggattat ttccaatcct tatcctttat ttagaattat agcacaggga ccataaaaaa
       61 cagggaatgg actttaaaga taaaaagagc tggcttaagc cagctctttt tatctttact
      121 acatactctt cattgaaata ctcgtctatg ctaaagcacc cattggatcc cacggagata
      181 gaacaattgg ttcttcattg aacgcattta attgctcttt cgtcaataaa tcttttctta
      241 caacgatttg gtacgtatat tcgtccatcc aggcatcact cataacaaag aagccattct
      301 ttccaacttt ttcgccccag ctattttcga ctttccattt tgttgattgc ccgtctacta
      361 agtcaacacc ggtcaatacc atcgcatggg tcatcagact ttctccaaaa tctaaacgtt
      421 cagctttagt cattgtaaaa tccgtatcaa atagatcatc cattgaataa gcatctaagg
      481 ccatgatgcc actagttcta gtagaagatt gacctacatc acaaccaaac caaacagatt
      541 ctccttcttc aagctgagca atcgctaatt ttttgaaagt ttccatatca acattcagat
      601 atttgacttc tttcccgcca acgacatttc ctagcatctc tactgtataa gaacgattga
      661 atggtttgtc ttttgttggt gcattgatca cgctaacata atcatctaat tttacaccga
      721 tgtatttatc ataaaaaatt tgtggtgtca gtcctcggtc aagatgatag ttcttttctt
      781 catcgcggta ctcaaaatcg aattcttttg gaggtgttcc taatgagata gctaagaagt
      841 tataaatgct ttctagcatt tcttctttct tttcttcaat agcgtctaat gtttctcctt
      901 gagcaaccat tttccgtaac actactgcat ctttacgcag taatttgttc aagtagttat
      961 tcaaatctcg agaatttgaa ctattcgcac tttccggcat caccgattta ggcacaacgc
     1021 catatttttg gaataatgaa acgatcatat cccattggcc gccatcttgt tgaggagtag
     1081 cgagtaagaa agctactttc cggctatcga ttgcctcttt tcctgttgca atgatgtttt
     1141 catagaaata atttgccttt tcatatttat cccagaaaaa agtgtaattt tgtgataatt
     1201 caaactcttt taaatggaat tgattcagca tcttatgacg gaatgtgttc agtgcagcaa
     1261 acatccagca acgccccgat tgcatttgat tagccacttt ccccgtagcc aaatctatag
     1321 aaaataccgg tgtattctcc acatgtgctg aaacattttc agcagaagca gtaattccgt
     1381 tcttcacaac tgcccgctgc aatgcctgct gtttgatatt tgcttgaaac tgtttgttaa
     1441 attttgttgt catatcaggc gtaatagctt tcatccaatc atccttccaa atccaagtta
     1501 tacttcgtat tttactctta agaatttaga aatacaatct cttctatgat gaaattttca
     1561 tttgaatgag aatatactta caacttcatg aagaaaaatg tcatacggta tacaatagtt
     1621 ttcagataaa aaggaatatt tctatgagat aacgaaaatg gcctgtataa taaagaagag
     1681 gcctagaaga acatcataca ataagatagc tttcaaaata ctcattgaaa gaaggaaata
     1741 ctataacgta agaaaggaga tgaataaaat gaaaaacctg attgctggcg gctttttgtt
     1801 tgtgggtggt ggcatactta tgaatacaga taactttggg aatgtcatcc cttttcttgg
     1861 tgcagcttgt gctttgattg gtgtaggaat gctgatttat ttcgtattta gcgatgatgg
     1921 gaagtatggt tgattatgag tagccaactc tactctttcg taaagattac aacatacgtg
     1981 acaaaaacag aaagcttgat tcatcataat aataaaagaa aaagaaaccc aagtgattgg
     2041 atttcttttt ctttgcataa gctttattta
//
LOCUS       DACCCS010000160         2025 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.171, whole genome shotgun sequence.
ACCESSION   DACCCS010000160 DACCCS010000000
VERSION     DACCCS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.171"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..153
                     /locus_tag="IVK82_002896"
     CDS             <1..153
                     /locus_tag="IVK82_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP6393892.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="IVK82_002897"
     CDS             159..575
                     /locus_tag="IVK82_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393893.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="IVK82_002898"
     CDS             681..944
                     /locus_tag="IVK82_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6393894.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..1216
                     /locus_tag="IVK82_002899"
     CDS             944..1216
                     /locus_tag="IVK82_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP6393895.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            1293..1622
                     /locus_tag="IVK82_002900"
     CDS             1293..1622
                     /locus_tag="IVK82_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6393896.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1612..1899
                     /locus_tag="IVK82_002901"
     CDS             1612..1899
                     /locus_tag="IVK82_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6393897.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          761 a          253 c          400 g          611 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcggcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga tttgaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacat atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggacgca
     1201 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
     1261 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
     1321 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
     1381 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
     1441 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
     1501 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
     1561 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
     1621 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa agctaaaaga gaagccattg
     1681 aaagaaaaat ttttgaattt aatttatgat gaaattgcga ttgatccata taagggtgaa
     1741 caaaaaactg gggatttatc aggtatttgg tctagtgggt tcaaatatgc gggcacaaca
     1801 tatagagtag cttacgagat tatagaaaac aaggttattc cagtattgtt atgtggaacg
     1861 catgaaaact tttatgaaca gttgaaaaaa ttaatctagt gatttgttgt cgttcttaag
     1921 aaggacggct tcttttttat cgaaaatctg attcgtctac atgactaaag tcgcgagtat
     1981 tctctgcttc tcgataaatt aaataacgtc attactttga ggaaa
//
LOCUS       DACCCS010000161         1977 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.172, whole genome shotgun sequence.
ACCESSION   DACCCS010000161 DACCCS010000000
VERSION     DACCCS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.172"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            13..251
                     /locus_tag="IVK82_002902"
                     /pseudo
     CDS             13..251
                     /locus_tag="IVK82_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            253..1551
                     /locus_tag="IVK82_002903"
     CDS             253..1551
                     /locus_tag="IVK82_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP6393898.1"
                     /translation="MKHTHKIILAVTSVCTVIILGVSIFFVTQAATNNKLENTSQTTQ
                     HSSSSSKPVEDKQTTKQLDQAKQLAASYHYDEAIALLEKDDAKEAQQLLATLKKEKES
                     LVKWEDPTKISHVFFHSLIVDPAKAFHTQQAQGYKDYMVTISEFNKTIDQLYKNNYVL
                     VNLNGLVKKGTDGKLTFTGVSLPEGKKPLILSQDDVSYYEYMDNSGFPSKLIVDKQNQ
                     IKNIYIDNKKETVGDYDMVPLIDSFIKKHPDFSYQGAKGTLALTGYNGVLGYRTSKSE
                     YGDNEKTHKEIEAAKKVADQLKEDGWSFASHTWGHLNMTQASLADIQQDNERWQNEVA
                     PILGKTNILIYPFGADISDWQPYSEANQKFAYLKQQGFDIFCNVDASTPAWGQLGTDY
                     YRNARINIDGIRFEADLKGENPILDHFINVKEMYDQKDRG"
BASE COUNT          713 a          345 c          360 g          559 t
ORIGIN      
        1 ttttagtttt tcttggaaat aaaaacagcg aaaatccgaa cattttcacc tctctcactt
       61 tataactttt acaaaatagt acttctcata aaaagcgctc tagttaaaga aaataagaga
      121 aaaatcaaaa aaaacaaaag tttcttttac acttgtttct atattcgtta caatggttat
      181 gggaagtttt taaaaaggtt aagaaaagct tacgggggtt tttgaaagca aaaagtagta
      241 ggggacgata aaatgaaaca tacacacaaa atcatattgg ctgtcacttc tgtatgcact
      301 gttattattc taggtgtaag tatctttttt gttacacaag cagcaacaaa caataaatta
      361 gaaaacacta gtcaaacaac tcaacactca agcagcagct caaaacctgt tgaagataaa
      421 caaacgacga aacagcttga tcaagcaaaa cagcttgctg caagttatca ttatgatgaa
      481 gccatcgcct tacttgaaaa agacgacgcc aaagaagcgc aacaattgtt agcaactttg
      541 aaaaaagaaa aagaatcctt ggtaaaatgg gaagacccga ccaaaatctc acacgtcttc
      601 tttcatagtc tgatcgttga tccagctaag gcttttcata cccagcaagc acaagggtat
      661 aaagattata tggtcactat ttccgaattc aataaaacca ttgatcagtt atataaaaat
      721 aactatgttc ttgttaatct aaatggatta gtcaaaaaag gaacagatgg aaaactgacc
      781 tttacaggtg tttctcttcc agaaggaaag aaaccgttaa ttctttcgca agacgatgtc
      841 agctattatg aatacatgga caattcagga tttccaagta agttgattgt cgataaacag
      901 aaccaaatca agaatattta tatcgacaat aagaaggaaa ctgttggtga ctatgatatg
      961 gttccattga tcgattcatt cattaaaaaa catcccgatt tttcttatca aggcgccaaa
     1021 ggaacattag ctttaacagg ctataatggt gtattaggtt accgtacatc aaaatcagaa
     1081 tatggtgata acgaaaaaac acacaaagaa atcgaagcag ccaaaaaagt tgctgatcaa
     1141 ttaaaggaag atggttggag ttttgcttct catacttggg gacacttgaa tatgacacaa
     1201 gcttctttag ccgatatcca acaagataat gaacgttggc aaaatgaagt tgcacctatt
     1261 ttaggcaaaa caaatatttt gatctaccca tttggcgctg acatcagcga ttggcaacct
     1321 tattctgaag caaatcaaaa atttgcttac ttgaaacaac aaggatttga tatcttctgc
     1381 aatgtcgatg cctcaactcc tgcatgggga caactaggaa ctgactacta ccgtaacgcc
     1441 agaatcaata ttgacggtat ccgtttcgaa gctgacctaa aaggagaaaa tccaatactt
     1501 gatcacttta tcaacgtcaa agaaatgtat gaccaaaaag atcgaggata gaggttgtga
     1561 aagaagcgtc cagctccgag caaataagaa ctagatttct aaaatagttt ctcatatttt
     1621 ggaaatctag cgcttattgc tgaggagttg cttcttgaac accgtgaata gaggttgtaa
     1681 aaagagcgtt ttgacttatt gttgaaggtc agctctttga acaccgcgtg ttaggttgtg
     1741 aaaaggctgg tctgcatcaa gcaataagaa tgaacagaga aaaacagctt cttgaatacc
     1801 gcagataggt tgtaaaaaaa ccgttttgac ctgacgataa ccagtaaaac agaggtgaga
     1861 ccattgctaa aagtagcagt ggtctcatct ctttttttgc acaagtcata tttgattgtt
     1921 tcgttatttt aatcaggata aagttctttt tttcatgcta aagagtgatt ctttctt
//
LOCUS       DACCCS010000162         1934 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.173, whole genome shotgun sequence.
ACCESSION   DACCCS010000162 DACCCS010000000
VERSION     DACCCS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.173"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..563)
                     /locus_tag="IVK82_002904"
     CDS             complement(<1..563)
                     /locus_tag="IVK82_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP6393899.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="IVK82_002905"
     CDS             complement(750..1400)
                     /locus_tag="IVK82_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393900.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
BASE COUNT          685 a          385 c          233 g          631 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttt
//
LOCUS       DACCCS010000163         1932 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.174, whole genome shotgun sequence.
ACCESSION   DACCCS010000163 DACCCS010000000
VERSION     DACCCS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.174"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..846
                     /gene="yidC"
                     /locus_tag="IVK82_002906"
     CDS             <1..846
                     /gene="yidC"
                     /locus_tag="IVK82_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP6393901.1"
                     /translation="GIVYRVLVHPMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPL
                     GISQSKKTMIQSEKMQALKPQVEAAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTG
                     GIGCLPLLIQMPIFSALYFTARYTEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYI
                     STIGIPEEQKKTMRTMLIVSPAMIVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMK
                     PRLKAQVAEELKQNPPKQVVTPRIDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGK
                     QQKRK"
     gene            complement(1025..1225)
                     /locus_tag="IVK82_002907"
     CDS             complement(1025..1225)
                     /locus_tag="IVK82_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393902.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT          601 a          417 c          320 g          594 t
ORIGIN      
        1 ggaatcgttt atcgagtact cgtccatcct atgggccaag cgattacata tctagttgaa
       61 aatttcaatt ggtcttatgg ctgggcagtt attgcaatga ctgtgatcgt ccgtatcatc
      121 atcttgccac taggtattag tcaatcgaaa aaaacaatga tccaatctga aaaaatgcaa
      181 gcgttaaaac cacaagttga agcagcccaa caaaaattga aagctgcaac aacacgtgaa
      241 gaacaaatgg cagcacaagc agaaatgcag caagtatacc gcgaaaacgg attgagtatg
      301 accggcggaa tcggctgtct gcctctattg atccaaatgc cgatcttctc tgccttgtac
      361 tttacggcac gttatacaga aggtatccgg gaatcatctt tctacggcat cgatttaggc
      421 tcaccaagta tggtacttgt tgccattgca ggggtcgctt atctgcttca aggatacatc
      481 tcaacgatcg gtattcctga agaacagaaa aagacgatgc gtacgatgct gatcgtctct
      541 cctgctatga tcgtcttcat gtcgatcagt gcgccagccg gtgttacact ttactgggta
      601 gtcggtggtg tgttcagctg tctgcaaaca ttcatcacaa acgttatgat gaaaccacgt
      661 ttgaaagcac aagtggcaga agagttgaaa caaaacccac caaaacaagt cgtcactcca
      721 agaattgatg tgacagaatc agctaaacca gctgacagca aacaagcaac caaacaaatc
      781 aagcaaccca aaaacagtac aaacaataaa ggccgcaacg caggaaagca gcagaagcgg
      841 aagtagatag taaataagag tgtaataaaa attcattaac agttttagca attaaaaata
      901 tcgaccaaga tttttgaaaa agaaatctgg tcgatatttt tatttaagtg aatgtttaat
      961 ttgaatcatc gattgatcgc tatctaattg cagcagcatc tttttatgaa gaaacaactt
     1021 tttcttatct atcaacgtag acaggtgttg ctgtttcttc taatagtgta cttaattcct
     1081 taatattttg ttttccttct tctaacatcc aactactcgc tttttcttcg ccttcggtaa
     1141 caaaaggcgc cactcccttc gcccatgttg ctcgcccaca taagacacca ttctccataa
     1201 aggacaacgc ggagatacca cccatctaaa taacatccat aaacctttct agtagaagaa
     1261 tagtgatagt atccatccct ttttctactg ggaatacaat tacacgattc aaagcttgat
     1321 acacaacatt tcccttctta taaaccgcta ttgtaaccga attgttttga atcacctttt
     1381 tgggatagaa actggctcct ttccgcaact tcttaaatgt tggtttcttt tatactttct
     1441 ttgtagcaac tacaaatttc ttcaaatctc taaatgtttg cataacaacc gcctttctga
     1501 cttttactca ggatggtatt taaaaatcag caaaataagg cttctctcca tcttttttat
     1561 ttcctagctg ctttaaagac actcttgctg ttttttaatc tctatcaagg gggctctgaa
     1621 tctattttga tgaacttatc ttattaattt ctcaaatgag ctgtcagaca caactgtatt
     1681 ttttcaatct cataccggac aactatgcca gcttaaaaaa atttcctctg gcaaaacttc
     1741 tttattgaaa aatgacaatg aaaaaagacc attctttttg ttgaatgctc attgtgtaaa
     1801 catatttcct gcgactgctc ttcgaaccat tctcagtgca atccctaatt tatctattaa
     1861 atcttctttt agttcctcat ctttcatatt caaaatttcc tttgtatgtt acacaagttt
     1921 gttctctaaa ta
//
LOCUS       DACCCS010000164         1929 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.175, whole genome shotgun sequence.
ACCESSION   DACCCS010000164 DACCCS010000000
VERSION     DACCCS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.175"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(63..230)
                     /locus_tag="IVK82_002908"
     CDS             complement(63..230)
                     /locus_tag="IVK82_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP6393903.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            complement(426..632)
                     /locus_tag="IVK82_002909"
     CDS             complement(426..632)
                     /locus_tag="IVK82_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393904.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            1086..1361
                     /locus_tag="IVK82_002910"
     CDS             1086..1361
                     /locus_tag="IVK82_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393905.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            complement(1819..>1929)
                     /locus_tag="IVK82_002911"
     CDS             complement(1819..>1929)
                     /locus_tag="IVK82_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP6393906.1"
                     /translation="ISREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
BASE COUNT          599 a          381 c          298 g          651 t
ORIGIN      
        1 tcaactgtaa tagccatcta tagaattcct cggtcatgtt ccgtctcctt tcgcaatctt
       61 atttaatgct tagctattct tttgccatac aatggaataa cttcattgtt ttcctttcgt
      121 ttatatgtat cgctctttat tggtcttcta tactcatata ctttttcgcc attaccttta
      181 tgaacagtga ttacttcata cttctgttct aagtattgtg gtctatacat tgttgtttcc
      241 tcctttgtgc aaaaataaaa agaccactca aagagtgatc taatatgtaa tagtaacttg
      301 cacgatgcac aaaacgcgta cgaaattgcg cacccctata tttttaaacc gccgatgcct
      361 cggttgctta aagtcgctgg agtgggattg caccactcac gagaacttac caggctctca
      421 cgaggctact cgccatttac cgctgcgtct tctacttccg ccacagtgac cgaagcttgg
      481 tggtgtacag atagcacact tactacattg ccgtacgtag caccgtatag cttcttaacg
      541 atcttttttc ggtagtcgta accgtcatag gcatttaatg tcgctggcaa ggaatcgaac
      601 cttgcatggt tgccgaagca ttgacctagc acacatgctt agcgtctacc ctttccgcca
      661 cagtgacact ataaaattat tcttggctgc tactattttt tattttgccc atttttaaat
      721 ccaatcatat agacattaag acagagcgca aaaattgaaa ttattaatgg aatcatttct
      781 cttcacccac ctttagttat cgtgtgaata attaaaaaac aatagacagc aacaaaataa
      841 cattgctttg ataatttggt ataaaccact ataaatttct tttcttgcaa ttatttttaa
      901 tatatgctag attatcaacc gatatagtca ctgcctgtac tagcggaaac tagtgcaggt
      961 tttttgttct atttactcaa aagttattac gataaattaa tattgtgaaa ataaatacta
     1021 agcgtataat tttatttatc agcgagtggt ccgctgaaat ataaaacaag gatgtgcaag
     1081 acaacatgta taaaccctac atgattagtt atgatcttaa taacccaggg caaaagtatg
     1141 ataaagtatt tgaaataata aaagaatttg gggcatatat aaagctacaa aaatcttttt
     1201 ggttagttaa aactaatttg aatccaaatc aaatgtgtga aaaattaaat acagtacttg
     1261 ataataacga ttccttattc atctgtgaac tgcaaaaaaa ttatcaaggt agagctacag
     1321 aggaaaattg gaaattcatt aacgaacata ttttctctta gtaaggatta gatatttttt
     1381 ctctgtttaa acaattgcaa ataccgtcaa tatctgaatt agaaacactt accttttgct
     1441 ccttgctact accaatagct tggagcaatt cttgtatttc ttctggagtg ccttctactg
     1501 ataatttcat ttttcttccc tccaatacat aaattaatag acagcagcag ttgaaagacg
     1561 acaagaacat gtttagtttt gtgagtgctg ctgtctaatc aaaataaaca gcaaccgatg
     1621 aagaatttag gaggagttga attcacatcc ttttcttcat aattagttgc tgtctatcta
     1681 agaagaagtt taaaacgatg agggagattg cctcccttcg tttattttgt cgatcctgtt
     1741 tcctaatctt tcgacactac cataataaca ctggtaaatg tctaaaaacc gccatcattc
     1801 cgccaaaaaa ccgccaaatt atttataagc aattattttt ccgtgtttat atgcttctgc
     1861 aaactctatt agagcttccg acttcatccg ttgtatactt ctttctgaat aacccacttc
     1921 acggctaat
//
LOCUS       DACCCS010000165         1902 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.176, whole genome shotgun sequence.
ACCESSION   DACCCS010000165 DACCCS010000000
VERSION     DACCCS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.176"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            175..597
                     /locus_tag="IVK82_002912"
     CDS             175..597
                     /locus_tag="IVK82_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393907.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            607..810
                     /locus_tag="IVK82_002913"
     CDS             607..810
                     /locus_tag="IVK82_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393908.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1021..1605
                     /locus_tag="IVK82_002914"
     CDS             1021..1605
                     /locus_tag="IVK82_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6393909.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1774..>1902
                     /locus_tag="IVK82_002915"
     CDS             1774..>1902
                     /locus_tag="IVK82_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP6393910.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSR"
BASE COUNT          675 a          259 c          340 g          628 t
ORIGIN      
        1 gtcacttttc tttttaaatg gcaatatttt ttgtaaataa atgtcaatca agaaaattta
       61 aacgattatg acattaaaaa tatattttat gaattttcta gtatataact tgatttatct
      121 gttaagattt attaagaaaa cgttttctta tttttgattt cgaggtgata gacaatgaaa
      181 aataagttta ttttatcttt aggtatttta actattggaa ttagtgtaac tggaactaca
      241 attcctacat ttgctgatag tacaaatgaa ccaactactg tagtagataa taaccaagaa
      301 ttaactcctt tagataggaa cggaatagcc attggtaacc caactaacga cttaactgtt
      361 actgaaaatt atttaatagt ttctccatta cttgctcgag cagcttctaa aacgatacga
      421 gtgacaggaa cgatgatttt tgataaagtt ggttggcctt ctagacctcc tcaatcacgg
      481 aattggaaag aaaaaagaaa tggttattgg tactatggaa aattgtattt acaaagttat
      541 aaatcagctg gaaacaattg gattgcgact tattcaggaa ccttaactag aaagtaggga
      601 ttttttatgc cagaatttgt gaacacacca aatttttatc aagatagcga tattctaagt
      661 aaaattaatt taccgaaaga agaatcagct ttacaagttc agcgaacttt acaggagaca
      721 caaaaaattc aaaaatcaat ttctaatcta gaagattctg aagaaaaaag aaaaaaaaga
      781 gaccaaaaaa catatcaaaa agaaaaataa tgattttaaa aaaagagtgt ctagtcgaca
      841 ttcttttttt ctaattgaaa gtgttcataa aatcacccat ttctggacac tttctttttt
      901 tgctttattt ttcaaaaaaa caattcattt catgttcata aacccataac aatatctatg
      961 ttcagttaaa ctatatggat aaagttatgt tacaataaaa ttaatgaagg agtgattttt
     1021 ttgaaaatag gatacgcccg ggtatctact ggtttacaaa atttggattt acaagaagac
     1081 agcctaaata aatttggttg tgaaaaaatt tttactgatc atatgagtgg ggcaaaaagt
     1141 aatcgaccag gattagaaat ggccattgat tttgttcgct ctggcgatac acttgtcgtt
     1201 tggcgcttag atcgtttagg tagaaatatg gaagatttga tttcaatcgt caatcgactg
     1261 aatgagcgtg gtgtgagttt tcatagtatt caagaaaata ttacgatgga caaatcaagt
     1321 tctacaggac agctcatgtt tcatttgttt gcggcatttg cagaatttga gcgtaatttg
     1381 atcctggaac gatccgcagc aggacgagca gctgctcgtg caagaggacg gtttggtggt
     1441 cgtcctgaaa aattgtctga acaagatttg gaattactga agactttagt ggatagcggt
     1501 acacctatta agactattgc ggaacggtgg aacgtctcaa gaaccaccat ttatcgttat
     1561 ttggcaaaaa tggaggagaa aaatggaaag acaaattctc attagttttt gtcatttaat
     1621 gctgaaaatt tttgccattt atctagaaaa gtgacaatag tatgagaatt ttttgtaagt
     1681 gtctactgat aacttattct caaatattta gctatttctt ctttacaaca tcgaacatat
     1741 gtttgtataa tgtggaagaa tggagtgtta tttatgatgg attattcaaa agagccagta
     1801 aatgattact ttttaatcga tatgaaaagc ttttacgcaa gtgtcgaatg tatagaaaga
     1861 aatttagatc cattaacaac agaacttgtt gttatgagtc ga
//
LOCUS       DACCCS010000166         1833 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.177, whole genome shotgun sequence.
ACCESSION   DACCCS010000166 DACCCS010000000
VERSION     DACCCS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.177"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(277..732)
                     /gene="tnpA"
                     /locus_tag="IVK82_002916"
     CDS             complement(277..732)
                     /gene="tnpA"
                     /locus_tag="IVK82_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP6393911.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            975..1833
                     /locus_tag="IVK82_002917"
                     /pseudo
     CDS             975..1833
                     /locus_tag="IVK82_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          580 a          357 c          345 g          551 t
ORIGIN      
        1 attatttctg cgccagcatt ttctaatgtt tgaatttgtg ttttgtaatc ttggttgctt
       61 gtgctaattc ttgaatagcc gtaaattgcc aaaatttcat caccctttct ggacataagt
      121 ttctgacacc agttaaaatc aagtctatca gtaacccata cacccgtgag gacgggtgta
      181 tttttaatcc ccctagaagg ggtcgacact cgctgcccct taaaagggga tttttaaaag
      241 accgccaact gctcatcttc actcacttac ttctctttat tttttaaatg gttctacgta
      301 ctctctttta cttataatat atcgaatgcg atcatcagct tcttgttcac ggatgtattt
      361 ttcatttgtc ttttgattca atccgactgt actcacatag tatctcttcg accaaaagga
      421 tttgtttccg tatttatatt tgagatttgc gtgctgctcg tggattagta ccgcactacg
      481 acctttcaga taccccataa aactggatat acatattttt ggtggtactc gaacaagcat
      541 gtgaatatga tctagcattg catgtgcttc tatgatttcc acatctttca tttcgcacaa
      601 cttcctaagt atttttccaa gatcttttcg taattgccca tagattactt ttcgttgata
      661 ttttggtgta aaaataatat gatacttaca attccaacga gtatatgcta aacttttatc
      721 gtcatttgac atgaaaaaac tcctttcgat ctgatgagcg gttggtagcc actcactagt
      781 atatcgaaag gagtttttat cgctaaagat acttcttcca cgcgtagaaa cgcgcggttt
      841 tcttgtttcg cacgctttac gcacaaaact gactgaagtc attaagaaaa taaaagggtc
      901 taaaactttt aagttttgta ccgaccccca aaagttagac caaaaaatct aacttttgga
      961 ggtcggtatt ttttatggct aagtatactt ttgaacttaa attaaagatc gttcatgatt
     1021 acttggatgg taaaggcgga agtgattatc tggcaaagaa atattccatt aaggctccat
     1081 cacaagtgaa gcgctggatc aatgcctatc aagaatttgg tgaagagggt ctagtaagaa
     1141 agcgacaaaa gaaaaaatat tctgttcaat ttaagctgga tgcgatagag ttatatctaa
     1201 caagtgagct gtcctatcgt gaagttgcca acacacttaa catgaacaac cctaatttga
     1261 tcgctagttg gatgcgtcaa ttccgtgagg gtgggatcga tggactctca aaaacgaaag
     1321 gatgtccacc tatcttgtct aagaaaaatg aacccaaaaa taagaaaaaa tctacaacta
     1381 aagcaacgtc taaagaacgt gaacgcatcg aggagttgga gaaacgagtc cgttcccttc
     1441 aaattgaaaa tgccttttta aaagaattga ggaagctgcg aaaacaggaa gcccaacaac
     1501 gacgaatgaa gcaatcgcac gaatcatcgc aagcctccga ggaccgttta aattagttga
     1561 gcttctagaa actttaaagt tccctaaggc aatctttatg tactggcaaa aacgattgga
     1621 ccgtaaaaat ccagatcaag agattgaagc ggaaatgctt aaagttcggg agaagcacaa
     1681 ggattatggc tgtttacgga tgaccaatga actaagaaac cgcggtttct ctatcaataa
     1741 aaagaaagtt cagcgcctga tcaagaagct aggaataaaa gtaacgacat acacacgtaa
     1801 atcacggcgc tacaattctt atcgaggaca agt
//
LOCUS       DACCCS010000167         1804 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.178, whole genome shotgun sequence.
ACCESSION   DACCCS010000167 DACCCS010000000
VERSION     DACCCS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.178"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..688
                     /locus_tag="IVK82_002918"
     CDS             <1..688
                     /locus_tag="IVK82_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HAP6393912.1"
                     /translation="CIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANED
                     LATDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKIL
                     AEFDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLC
                     NSYGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQL
                     QRLLDYRKDD"
     gene            complement(987..1706)
                     /locus_tag="IVK82_002919"
     CDS             complement(987..1706)
                     /locus_tag="IVK82_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP6393913.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
BASE COUNT          570 a          353 c          376 g          505 t
ORIGIN      
        1 ctgtatacct gtagatgaaa aggggcagct cttagcattt gaattagacc aatcttatcg
       61 cggaaaagaa ctgctgggat tttttgaaaa acagataact gaaagcgcaa aagatttgac
      121 tgcaaatgaa gatttggcga ccgatttgat ttggtatgcg tggagcgggc cattttcgcc
      181 tttattcggc aagagtaaaa tggcaacttt tgagcgctat tttctcacag agcctcatac
      241 gcacgtcgaa aaaagcaatc cctattatca cttgcgagat gaagagtggt tcagtgaaaa
      301 aattttggca gaattcgatg caaaagaaga aggttcagca atcatcaatg gacacacgcc
      361 agtaaaagta aaaaaaggag agtcaccgat caaagcaaac ggaaaagcat ttgttattga
      421 cggaggtcta tccaaagctt atcaaaaaac aaccgggatt gctggctact cattactatg
      481 caattcttac ggtttccaaa tcgtgacaca ttatccgttc ttgcctattg aacaacaatt
      541 tgcaaaaaaa tctgatcaaa cgaatgtaaa aaaagtgatc gagcagcagc tcccacgcaa
      601 acttaatcgg aatacgacaa aaggaatgga attacagcaa cagattactc aactccaaag
      661 gctgttggat tatcgaaagg acgattgaga aaaatgctag ccaacattgc tcaatagact
      721 aaaaagcgac tgcaacaaaa ggcgtgtcgc agtctccttc ggaattagct caaaaaacgg
      781 taaaatatta gaagctattt ttctgtttat ttctctaata ctcaggtcag ggcgctgatt
      841 tcacgacctc ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
      901 ccaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttgaacggca
      961 ggctcacgac ctaatcagaa aaacgattaa aattcattca caaagaaatc gaataaacgc
     1021 tgttcagatg aatctacacg atgagaaagt tccggatgcc attggacacc aaggattttg
     1081 tgcatattat ctttactttg gactgcttca atgagtccat cgttggaaaa ggcgatggct
     1141 tttaacaaag gagaaagatc tttgactgcc tgatgatgat aggaattcac aaagtatgaa
     1201 tcagggagaa ggtcacttaa taaactgtcc gaaacgattt tgacttcgtg agttgcaaac
     1261 tgcggggcag tgggctgttg ttcgtgtttt acggaccatt ttggatataa tgaaagatct
     1321 tgataaagtg ttccctccaa cacgacattc aatagttgaa gtcctcggca gactgcaaaa
     1381 atcggttttc tttgtttcaa tgcttccaag atcaatgcct gttcgaattg gtctcgttga
     1441 atatttgttt ccgtcagttt tggatgaggg tcttccatgt aaagttgcgg actaacatcc
     1501 tgaccgcctg caagaatgag tttgtcgatt ttctcaatta gttgaggcgc tgaatctgga
     1561 gacatgactg gcagaataag aggtaatcct tgagcatcta aaactgcatc cacaaatccc
     1621 tgcggtgtat agcttacttg atttccttgg aaagtatctg tcgctcgaat cagttgattc
     1681 ccggcaatgc cgatgattgg tttcattttt attcccccta aagtagacta attgtaacaa
     1741 ggatttaaaa aaatttaaac tatgtaagac ttgcccctat acttatctgt tcttgctgtg
     1801 ttac
//
LOCUS       DACCCS010000168         1753 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.179, whole genome shotgun sequence.
ACCESSION   DACCCS010000168 DACCCS010000000
VERSION     DACCCS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.179"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(193..570)
                     /locus_tag="IVK82_002920"
     CDS             complement(193..570)
                     /locus_tag="IVK82_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393914.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(870..1751)
                     /gene="galU"
                     /locus_tag="IVK82_002921"
     CDS             complement(870..1751)
                     /gene="galU"
                     /locus_tag="IVK82_002921"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP6393915.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          504 a          381 c          254 g          614 t
ORIGIN      
        1 aaaacattgg tatgatttat atatatataa ataacataaa aaaaggaatg gcttatcccg
       61 atacaacaag cattccttta aaaaagattc ttttatataa gtgaaaaaag cttttttcca
      121 ccaattaaat aaatctctca tctcaatagt ttatccatta ttttatcttt ttttgcgtat
      181 acctactaaa tattaccgtc ttcttctcgt tcttctactt tgttcgagag aatctgtact
      241 agaaaaagta gttgtatgtc taccttttct gcctatccga gactttcttt tcatatatgg
      301 gatataaatg attagataca gtacagcaaa aaataaagca aatacgcaag cttcccaact
      361 acctaacgcc aattcaacca tcaaggtagc aaccccaacg gatgctgcat accagataaa
      421 aatcagaaac gtttcttttt ttctgaccat gcgaaacatg ataaatagac taataagcaa
      481 tagtggcaac acaaatatcc aattgcttcc ttccatcagc cacttgagta tatcaagcag
      541 cgactgtgca acagcgacta attttcccat agtgaacaca cacctttttt tataattaaa
      601 aaattcgcct ttttattcta ctatgaatct tctggcttgg ctttcatttc tcttatgtaa
      661 tcattttctt ttatacaact taaaaaaaaa ttaaaccatt agcataatat tttctgaatc
      721 tattcacgaa agtctttttc tatctttcgt tttccacgac tcaactgatc tagagtcaaa
      781 aaactaaaac gacagttgcg caaatgacgc acaagctgcc gctttaataa caaatagcct
      841 ttcattctca gcaggccact tatttccagt taactttctg ttcttagctt tttactcaac
      901 tggataatat atgcacttaa tgaagtccca atttccgaat gttgaagtcc atattcaata
      961 tttgtcttca tatatcctaa cttatcgcct acgtcgtatc tttttccttt aaactctctc
     1021 gcatagaccg cctctgtctg gttaagagtc tgaagtgcat ccgttagctg tatctcgttt
     1081 cctgctcctg gttcttgagt ttctaatatc tcaaatattt taggagtcag tagataacgt
     1141 ccgataatgg ctaaattact tggtgctttt cccatttcag gtttttctac aaaccctttt
     1201 actctgcaac gacccttttc gctatctgtt ttaggatcaa taattccgta tttagatgtt
     1261 tcattttctg gtacacgcat gacggcaaca gcggaagctt gtgtttcttc ataatccatc
     1321 atcaattgtt gagtcaatgg gatatcatct ttcatcaaat catcccccag catgacgaca
     1381 aacggttcat ttcctataaa tgcttttgct tgaagcacgg catcccccag tcctcttgga
     1441 tgagattgac ggatgaagtg aagattgact tgagtcgttt cttcaactaa agcgagtaat
     1501 tctgtttttt ccttttcccg caaattgttt tccaattcga tatttgcatc aaagtgatct
     1561 tcaatgggtc ttttttcttt tcccgttacg atcaagatgt cttcgattcc cgaatccaat
     1621 gcttcttcca caataaactg gatcgttggt ttatccacga ttggaagcat ttcttttgcc
     1681 atggcttttg ttgccggcaa aaaacgtgtt cctagcccag cggcaggaat gactgctttt
     1741 ttgaccttca ctt
//
LOCUS       DACCCS010000169         1746 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.180, whole genome shotgun sequence.
ACCESSION   DACCCS010000169 DACCCS010000000
VERSION     DACCCS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.180"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            133..507
                     /locus_tag="IVK82_002922"
     CDS             133..507
                     /locus_tag="IVK82_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP6393916.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="IVK82_002923"
     CDS             complement(621..1250)
                     /locus_tag="IVK82_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6393917.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="IVK82_002924"
     tRNA            complement(1384..1456)
                     /locus_tag="IVK82_002924"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
     gene            1670..1743
                     /locus_tag="IVK82_002925"
     tRNA            1670..1743
                     /locus_tag="IVK82_002925"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1704..1706,aa:Asn,seq:gtt)
BASE COUNT          602 a          256 c          307 g          581 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaatgtatt
     1501 tgtcaacaga attttttcga aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa
     1561 aataaaaata acaaaaaatg cgaacggttt ctttgataat ataataaaaa aattcaaaaa
     1621 aagtattgcg ttatttagaa gaaattgata ttatattcat gtacttgatt ccggcatagc
     1681 tcagttggta gtagcgcatg actgttaatc atgatgtcgt aggttcgagt cctactgccg
     1741 gagtaa
//
LOCUS       DACCCS010000170         1700 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.181, whole genome shotgun sequence.
ACCESSION   DACCCS010000170 DACCCS010000000
VERSION     DACCCS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.181"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            466..1065
                     /locus_tag="IVK82_002926"
     CDS             466..1065
                     /locus_tag="IVK82_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6393918.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1129..>1700
                     /locus_tag="IVK82_002927"
     CDS             1129..>1700
                     /locus_tag="IVK82_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393919.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT          612 a          257 c          321 g          510 t
ORIGIN      
        1 acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat aatgatgaaa tcatttagtt
       61 cttataaatt agaaaagaga aaataaatat tccatatcta ctttattgga tccgtaacat
      121 gactaaattc tgaatacttt ttaaagctta tcatttggta aaactgaata taagttaatt
      181 atatagactg aaaattttga attttcagaa tagaataact taaaagtact gacaaaattt
      241 tttaaagggg caatattaat gttctgagat aatcaaatcc taccgaaaat atcaaaaatc
      301 caatgtgaca caatcagatt gtgtctcatt cgagggaatc acacaatccc acgtggctct
      361 aggcagaatt gaatttttgc ttggggcttt ttccttatat tgattttgaa tgagacttat
      421 tatatgttat aatgcaatta agtatcattg gagagggcga agaaaatgaa gacggtagaa
      481 cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa tcttggcttc gggaaaatat
      541 ggccaacgaa atttggttct tttttcgatt gggatcaaca ccgcctatcg catttctgat
      601 ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac gcggtcgcgt ggtggtcaaa
      661 gatcgattag cgatgaagga acaaaaaaca gctaaacata attcggtcta tatttccaac
      721 aaattgcgaa aaacgatttt ggagtacgtt caacaggaat ttcctgatca actccttcag
      781 caagattttg acggttattt gttccctagt cgaaaaggga cgaatgaacc gctcaaccgc
      841 caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa tcggattgaa agacattggc
      901 ccacattcca tgcgaaaaac gttcggttac tttctctata aacaggggac caaaacagaa
      961 atcattcagt ccttgttgaa ccattcttct cagcgcgaaa cattgcggta tattggtatt
     1021 acacaagaag acaaagatac agctgtaaaa agcttagatt tataatataa aagttaagtt
     1081 taaacatgga tagttttaaa ttagtagatt gagtgttggg aggaaagaat gagtacatta
     1141 tatattatag gaaacggttt tgatttaagt catggtttaa aaactagata cactgatttt
     1201 tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata aaatagacac aatgataaat
     1261 gaaaataatg aggtgagaag tacagctgat gataagatag gttggtttaa ttttgaaagt
     1321 agtttgcatg agttaattca aagtccagtc tttgaagatt atgtagaaga gtctaaggat
     1381 tatctcatag gttatggaca tgaagattgg tcagattctg cccatcatga ttttcaaaat
     1441 atggtttatg attatgttgt ttctctagaa gaattacaac aacttttcaa ggaatggata
     1501 cgcaaagaaa tacagccgac aatagaacat aaaattccaa aataccaatt ttcgtccgat
     1561 gtactattct ttacattcaa ttatacagat gttttggaaa ttctatacaa gcaaccgaaa
     1621 gaaaacatat atcatattca tggcagttat gacgatataa taatcggcca ctccgagaat
     1681 tttgacaact ttataggaga
//
LOCUS       DACCCS010000171         1668 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.182, whole genome shotgun sequence.
ACCESSION   DACCCS010000171 DACCCS010000000
VERSION     DACCCS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.182"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(369..1019)
                     /locus_tag="IVK82_002928"
     CDS             complement(369..1019)
                     /locus_tag="IVK82_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393920.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(1119..1628)
                     /locus_tag="IVK82_002929"
     CDS             complement(1119..1628)
                     /locus_tag="IVK82_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP6393921.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
BASE COUNT          511 a          338 c          203 g          616 t
ORIGIN      
        1 catgtttata tccaccattc aagttatttt atcagcaaat caataaaaac tggaatagaa
       61 tcccaatttc atcgtaagag aaactagact agcaataaga aaatagaaac attcatagaa
      121 aaaacctcac tcctctgatt ataaaagagg gggcattaac ctcgtgtata ctaaaacctt
      181 ccattcattc gatggatgat tttctttttg tgaaaaaata ttcattgtag tacgatgttg
      241 cgcaacacag acggatggta ttgcttatta tttattaaaa tcgaacaata cacttattta
      301 aaaacttctt tcccagattt tcttccattt ttacagttat aactagtatt atcatgaaaa
      361 atagcctttt attgtctatg ggaataataa tttaaaattg gtactttttt attaattagt
      421 tcatcgccca ataatctctc cagtttctta gcatctcctg actcaaccgg aattatccaa
      481 gcccagtcca aataattttt taaaatctca tcatattttg caaatatttc attgccatat
      541 tccgtcctta aaattatatt ttcagtgcca gacaagtgat ttgtattgag ttgataaaaa
      601 agatctccat ctaaaaaaat atcattagaa gaagatttcc ccacagtaat gaccattcca
      661 tcagctctat gtgttacata caaataattg tatgacctca tttcacctaa tgaatcaatt
      721 atttcttgcc ctttagctct aatatattga tctctattac ctatctcact cttatttaac
      781 aaaagttttt tcatgtcaaa tttaatcggc tgttttattt ttgtaaattc gcttctacta
      841 ctaatccaat ccgagattaa tttttcaaca tgtcccagag taaactctga tttattcgct
      901 aaatactttt tttgttgtat taaagacatc tttccccgaa gctcaaaatt gagcctacat
      961 cgattctcta tctcttcaaa aagcatctta tcttcatcat tcttatattc atcattcata
     1021 aaaccaatcc agctcctcga ttaagataat attttaatct catacaaaaa ctagctctca
     1081 tcaaaacaac cattgttttg atttctcatg aaatttcttt atacttttct ccgattataa
     1141 attcctaacc ccttgagaaa attacgcata tatttatctc cgcaaggctt aaaatttcgt
     1201 tggccttctt ttctcaaaaa tgccccgatt tcgccttttg atggatagag ttctgcctca
     1261 gcaaaaactt ctaaaatatc atcagtggtc atagacaagg cgatttttaa tttttttagc
     1321 aacacattgt tgatatattt gatttccttg cttttttgcg gcgtgtctac tatcggctta
     1381 tctttttgcg gaccacgcgc caagagaatc aggccattta ggaatgcgtc aaaaacttgg
     1441 ttgttgatgg ttttttcata cacattattt tctgcattat ctttgttctt atcctgtact
     1501 ttggtcagta ccgcctgagc ttcttcttta gtcagtggta caccgcctag ctcaaaagct
     1561 ttaatcaaat ccgtatcttt caaatcaatc gcataacgca atcgcaataa cctatcattg
     1621 ttattcattt gttcctccga agtatttagt ctttactttc ttacgtac
//
LOCUS       DACCCS010000172         1596 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.183, whole genome shotgun sequence.
ACCESSION   DACCCS010000172 DACCCS010000000
VERSION     DACCCS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.183"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(13..474)
                     /locus_tag="IVK82_002930"
     CDS             complement(13..474)
                     /locus_tag="IVK82_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP6393922.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            complement(456..1430)
                     /locus_tag="IVK82_002931"
     CDS             complement(456..1430)
                     /locus_tag="IVK82_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6393923.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
BASE COUNT          522 a          293 c          208 g          573 t
ORIGIN      
        1 gaaattgcca ccttaactga catcttttaa aagcgaatta atatgaacca tacttttttg
       61 agagttattc agtaattcct taaaatctat ttcttctata gatttaccag ctgactgggc
      121 cattaataac tccagtaaaa gaggtaaatt aatcccagca ataatttgaa ttctattgtt
      181 gttcaactgt ttaatagaaa cattattagg tgttcctcca attaaatccg ttaaaataat
      241 tagaggttgc aaatgatgct gactaattat ttctttcata ctctcctcaa aatcatctgg
      301 actagtattt tctttaaatg gaacagcact aatacctgtg gcgtcattca ttaaaattaa
      361 ttttgttgcc tttaaataac cctcacaaag ggtagaatgt gttgtaatca caatgtgaaa
      421 taattgattt gccatctttt gcaacctcct attaattaat atttcatttg ccacatatag
      481 cgtctaacat ctaaggaatg gcctctttca aaagcaatcg cttctaccat ttggcgaata
      541 acaacgccac taagtacagc tgcaatatat tctttcgcta cactatctat agattctaag
      601 tcaaaatcag cttggtctaa aaccattaat ttattactga attttttagt gaagtcttct
      661 acccgttgat ctaaaaatct tgtatctcca atacttttca ctaagatgaa tggtacatca
      721 taatcagtga tttcaaaagg accatggaaa aattccccag aatgaatagc attagaatta
      781 atccattgca tttccatgaa ccagcaagct gctgtggagt aaatttctcc gtaattaata
      841 ccgctaccaa ttgtgtaaat aattttatct cgtttttggt ttttacccca ttctttagca
      901 gttatttcaa atttgctctt ttcagcaata acaagatcat ttaacttatc aaattcattc
      961 aagcaagttt cccatttttt agagccatct aaaacaagta aaatattaaa taataaacca
     1021 taaagaattc ctttatttaa atcactagca tcaacatctt taccccaatc atagtgaaca
     1081 ggatattcag ctgcttccca tagaggagat ccttcttcat tagataaaca aatagtcatt
     1141 gctccaaatt ctcttgcttt ttttgttgct tctacagttt ctggtgttgt gccagaatgg
     1201 gaacaagtga taactaaaga attttcagat aatccttttg gtatagcata gttaaattcg
     1261 ttagcagtat aaacatgaca aggtacagtt aattctttat caaatagata ttgaccagcc
     1321 attaaaacgg cttgtgaacc cccacatgct gtaaaatata catgatcaat tgtcttttcc
     1381 tttaacgctg ccattgctgt ttcaatctgt tctttgtgta gttgtctcat ttctttatcc
     1441 ctcactctaa taggtattat aaagttatat aactttacga tacctattat ctatgaaatt
     1501 gctttatttt gcaatactat ttttcaaaaa aattttttat aacaatttta tataataaaa
     1561 tgccagactg tagacaaagt ccattttatg gatgat
//
LOCUS       DACCCS010000173         1594 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.184, whole genome shotgun sequence.
ACCESSION   DACCCS010000173 DACCCS010000000
VERSION     DACCCS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.184"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1485
                     /locus_tag="IVK82_002932"
     CDS             <1..1485
                     /locus_tag="IVK82_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6393924.1"
                     /translation="CEAKFSENSYGFRPNRSVENAIKATYNRLQISQLHYVIEFDIKG
                     FFDNVNHSKLIKQIWAMGIRDKHLIFILKRILKAPIKMTNGTITYPEKGTPQGGIISP
                     LLANIVLNELDHWVESQWQENPVTKNYVVHINKSGSPCKSNAYKEMKKTKLKEMYMVR
                     YADDFRVFCRYKESAEKAKIAITQWIEQRLKLEVSQEKTRIVNVRKRYSDFLGFKIKM
                     IPRRKKLVVKSHISDKQFKNQKDKLVSQAKKIASPSKGKTELEETRLYNSMVLGMQNY
                     YQIATNVNLDCMKLNRAVMAIFTNRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYV
                     AGEGEPIFPVGYIQHRKPMAKVYLANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENR
                     TIEYADNRISLFSAQHGKCAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAV
                     HRLIHAKTEETIQKYIALLKLNKVQLIRLNKYRVLAGNQELNLI"
BASE COUNT          632 a          251 c          307 g          404 t
ORIGIN      
        1 tgcgaagcta aatttagtga aaacagctat ggttttagac ctaataggtc agttgaaaat
       61 gcaataaaag caacctataa ccgcctacaa atcagccagc tacattatgt gattgaattt
      121 gatattaagg gtttctttga caacgtgaac cattccaagc tgataaaaca gatatgggct
      181 atgggaatac gggacaagca cttgatattc attttaaaaa ggatattaaa agctccaatc
      241 aaaatgacta acggtacaat aacatatcca gagaaaggaa ctccgcaagg gggaataata
      301 tccccactac tagcaaacat tgttcttaat gagttagacc attgggtaga aagccaatgg
      361 caggaaaatc ctgttacaaa aaactatgtg gttcatatca ataaaagcgg tagcccatgt
      421 aaaagcaacg cttacaaaga aatgaagaaa acaaagttaa aggaaatgta tatggttcgt
      481 tatgcagatg attttagagt tttctgccgc tacaaggaaa gtgctgaaaa agccaaaata
      541 gcaatcactc aatggattga acaacgatta aaattggaag tatcacaaga aaaaacaaga
      601 attgtaaatg ttagaaaaag atactccgac tttcttggtt tcaagattaa aatgataccg
      661 agaagaaaga agttagttgt taaatcacat ataagtgata aacaattcaa aaaccaaaaa
      721 gacaagctgg tatcacaggc aaagaaaatt gcttctccct caaaagggaa aacggaatta
      781 gaagaaacaa gactttataa ttcaatggta ttaggtatgc aaaattatta tcagattgca
      841 acgaatgtaa atctggattg tatgaaactg aatagagctg taatggctat cttcactaac
      901 agattaggta caaacaaaag gggcagattg agaaaaacag gcagacaact tacgacaaag
      961 gaggcaacaa gatatggtaa atctaaaatg cttcggtatg ttgctggcga gggcgaacct
     1021 attttccctg ttggatatat ccaacacaga aaaccaatgg caaaggtgta tttagcgaat
     1081 tgctacactc tagagggaag aaaatttatc cacaccaatc taagtgttga taaattactg
     1141 atgtaccagt taatgaaatt atctttggaa aatcgcacaa tagaatacgc agacaatcga
     1201 atttcgctat tttctgctca acatggaaaa tgtgctataa ctctggaaga atttcaacaa
     1261 gctaatgaaa tacattgtca tcatattgta ccaactggag ctggtggaaa tgacgattat
     1321 aaaaatctta ttttggtaaa agaggcggta caccgtctaa tacacgctaa aacagaagaa
     1381 acaatacaaa aatatatagc tctattgaag ctaaataaag tccagctcat aagactaaat
     1441 aaatatagag tattagctgg caatcaagaa ttaaacttga tataggtcta ctacataaat
     1501 acttttcaat ataaacacga tggaacgcgg agtgcggtga aagtcgcacg ctccgtgtga
     1561 atggggggaa aagtttgaga tatttataat atca
//
LOCUS       DACCCS010000174         1579 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.185, whole genome shotgun sequence.
ACCESSION   DACCCS010000174 DACCCS010000000
VERSION     DACCCS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.185"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            129..1298
                     /locus_tag="IVK82_002933"
     CDS             129..1298
                     /locus_tag="IVK82_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6393925.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          567 a          254 c          258 g          500 t
ORIGIN      
        1 aatatcccat attttaaaat aaaagatagc gtaaaatatt ttgtgtaaat agaaatttag
       61 ggtagaaaaa aaagtccatt ctgtaaaatt aaagttacca cacagaaatt ttataggagg
      121 actttctcat gactaaattt actatagaaa ttatgcaaac actactcaat aaaggggatt
      181 tagatgaatt atttcaggat catttagagc gagcgattaa ttcacttcta caagcagagc
      241 taactgcttt tttagattac gaaaaatatg atcgaaatgg attcaattca ggaaattccc
      301 gtaacgggaa ttattcacgt acatttaaaa cagaatatgg cgagttgaat ttaactattc
      361 ctagagatcg gaatggcgaa ttttctcaac aaacactacc agcatataag cgaaccaatg
      421 attcattaga aacgaccatt atccaattat ttcaaaaagg cattactatg gctgaaatct
      481 ccaaattaat tgaaaaaatg tatggtcacc actatacgcc acaaacaatc tccaacatga
      541 gtaaattggt ggctgaagat gttttagctt ttaaagaaag aacgttagaa gccaattatt
      601 ccgttatatt tatggatgcg acgcatatcc ctgtgaagcg acagactgtt tcgaaggaag
      661 cagtgtatat tacgattggt attcgtttgg atggaaccaa agaagttctt gggtttacta
      721 ttgccccaac tgagtctgct tatatttgga aagaagttct tcaagatctt agaaaacgtg
      781 ggttagaaga agttttatta gtagtgacag acggattaag ctgtattgaa gaaagtatcc
      841 atagtgtgta tccgaatgcc caatttcaac aatgttgtgt gcatgtatct agaaatatcg
      901 ctcataaagt tcgtgttcga ggtcgaaaag aaatttgtga ggatttcaaa ttggtttacc
      961 aagcgaattc aaaagaagag gcattggatc acatcgactt tatgactagg aaatggaaaa
     1021 agcagtatcc aagagtcgtc aatttactct tgaatcctgc cctattaacc ttttataatt
     1081 tccctcacgc catcagacga acaatttatt cgacgaacct gattgaaggc tttaataagc
     1141 agctaaaacg atatactcga agaaaagaac aattccctaa tgaagaatct ctagagagat
     1201 tcttcatttc tcaatttaat caatataacc aaaaattttt aggtagaatt cacaagggat
     1261 ttaaagaaat tcaggataca ttagagtcga tgatttaact gtaattaaca gaatggattt
     1321 tccatttaca tataattctt gacgctacca taatctaaat aaccatcata ttcatctact
     1381 acttatataa ttgagtttta ttaaactttt taagtttcta ttctatcaaa tatttaaaca
     1441 atctctgcat ccgaaaagac tacctcgtat agttttacta acaatatttt tattagcgtg
     1501 ttcaaatcct gatttcttgt atcaattatt ttctgttcac tttcaataag aaaaaagtct
     1561 ttacttccat ggataataa
//
LOCUS       DACCCS010000175         1573 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.186, whole genome shotgun sequence.
ACCESSION   DACCCS010000175 DACCCS010000000
VERSION     DACCCS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.186"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            287..694
                     /locus_tag="IVK82_002934"
     CDS             287..694
                     /locus_tag="IVK82_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393926.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IVK82_002935"
     CDS             708..1109
                     /locus_tag="IVK82_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393927.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IVK82_002936"
     CDS             1111..1482
                     /locus_tag="IVK82_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393928.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          593 a          195 c          271 g          514 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gcc
//
LOCUS       DACCCS010000176         1502 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.187, whole genome shotgun sequence.
ACCESSION   DACCCS010000176 DACCCS010000000
VERSION     DACCCS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.187"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(312..611)
                     /locus_tag="IVK82_002937"
     CDS             complement(312..611)
                     /locus_tag="IVK82_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393929.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(617..847)
                     /locus_tag="IVK82_002938"
     CDS             complement(617..847)
                     /locus_tag="IVK82_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393930.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(1097..1297)
                     /locus_tag="IVK82_002939"
     CDS             complement(1097..1297)
                     /locus_tag="IVK82_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6393931.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          533 a          243 c          219 g          507 t
ORIGIN      
        1 attgtgagat aataataaag aagagtttaa agcgttcccc aaaaaccact gccccataag
       61 tgtgttacgc tttaaactct tttatatttg aagccattaa aaagcatacc atatttttga
      121 aaaaaagtga gaaaaaaggc ttataattgg agtggtagtt aattagtgac ttatttttga
      181 ttttatagca ctgatactat aaaatataga tatcatcata ttacacaatc ttaatactaa
      241 cttaaaaaat atctcctttc ataagtatgg tgataaaatc cgttccgggc taccttttta
      301 ggtagcctac tttaatcttt gtatctttct ggatcaacga aagtatactt tatatagtca
      361 taacgccgat gatcgcttcg agcgtctagc acatcagtca cgatatcaaa caaaaaatat
      421 acgtctttct tcattctagt tttcgcagca gggattttaa agtagttctt attagaatag
      481 tagagattaa ttaataagct atcttcgatt gctaaaaaga aaacttctga atcccacacc
      541 ttataaaaat ctttgacaaa tctattcgaa ggatcaaatt taaaccataa ttgcgtctca
      601 ttaaaaagca taaccattac tccgatctat ttttaaactt agtctctacc tctaatatat
      661 atcgagtttt tattttgcct tcagagaata ctgtttcttt ttttgcagtt acaggttgtt
      721 tattttcgga aaaagctaat atagctaaaa ttgaaacatc catctggaat ttgtcttttt
      781 cgttgctttg ctcataaaaa tctgcatatt catcactaat attttttcta ataaattctt
      841 ccatcataaa gatcacctcg aaaagagtat acgaacaaac gttctttttg taaaggtgga
      901 atttattata ctaaataaat aggtgaacaa gtacttgtgc caaattatgt gccaaaaaaa
      961 atcgaattta ataaaacaca aacaaaaaga aatcctatta tgataacatt tcttataata
     1021 acaaacacaa cagaagacgt gtaatagtta gtcaggaacg tacaaataac ccctgtatcc
     1081 tttgcggtac agggggttat tttgtataag tagacattgt agtttctaaa ctgctatgtc
     1141 ctaaacgttt cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgcg
     1201 tatgtcttag tccatcaata aacagtcgaa gagaaaatag ggaaaaattg attactaaag
     1261 aaaaacttac taggtcgacg gtgtagttga ttttcatact tcaatagatt cgaattaaac
     1321 gatttcgatt ggcttatacc agtcctatcc aacgaaagat atatcgttct taactctagc
     1381 ggtataactt gaagctttac gtaaagccaa tgggaatgaa ctggagatga ctttttatta
     1441 tttttagtaa tagctgatgg gttaatactt acaactttcc ttttcaaagt accatataaa
     1501 aa
//
LOCUS       DACCCS010000177         1456 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.189, whole genome shotgun sequence.
ACCESSION   DACCCS010000177 DACCCS010000000
VERSION     DACCCS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.189"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            108..1040
                     /locus_tag="IVK82_002940"
     CDS             108..1040
                     /locus_tag="IVK82_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP6393932.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="IVK82_002941"
     CDS             1112..>1456
                     /locus_tag="IVK82_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393933.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DACCCS010000178         1451 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.190, whole genome shotgun sequence.
ACCESSION   DACCCS010000178 DACCCS010000000
VERSION     DACCCS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.190"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            105..1406
                     /locus_tag="IVK82_002942"
     CDS             105..1406
                     /locus_tag="IVK82_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6393934.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          476 a          293 c          264 g          418 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
     1441 ctcactctat c
//
LOCUS       DACCCS010000179         1424 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.191, whole genome shotgun sequence.
ACCESSION   DACCCS010000179 DACCCS010000000
VERSION     DACCCS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.191"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(150..1337)
                     /locus_tag="IVK82_002943"
     CDS             complement(150..1337)
                     /locus_tag="IVK82_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP6393935.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACCCS010000180         1423 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.192, whole genome shotgun sequence.
ACCESSION   DACCCS010000180 DACCCS010000000
VERSION     DACCCS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.192"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            96..1391
                     /locus_tag="IVK82_002944"
     CDS             96..1391
                     /locus_tag="IVK82_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6393936.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          450 a          281 c          266 g          426 t
ORIGIN      
        1 cttcaccaac atcttttttg tataaaaaaa tatagaaaag acaccctgaa atcctttaga
       61 attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag cttatcaaag
      121 ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta actaaagaaa
      181 aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag ccagaagagt
      241 gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg acaaccgttc
      301 ggttgttact gaatgatgtg tctgagtatc gcacttatct ggagctgaag aaacaacgat
      361 ttaaatgtaa gtcttgccaa agaacattcg tagcggatac ctctgttgct gaaaaacact
      421 gtttcattag tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag aatacttcca
      481 tgacggaaat cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt gtcatgaagc
      541 gattctaccg cccattgaat cctttcaaac agactttgcc aaaggtctta tgctttgatg
      601 aattcaaatc ggttcgaagt gttgccagtg caatgagttt tatcatgatg gacggacaaa
      661 gccatgcatt attggatatt gtcgaaaatc gacgcttgcc ttatttagaa cgctattttt
      721 ctcggttttc attagcgact cgtgaagccg ttcaattgat tgttattgat atgtatgcgc
      781 cttatgtttc gttggtgaaa aagcttttcc cgaatgctca gttgatcatc gatcgtttcc
      841 acatcgtcca acatattggc agaactttct tgaatcatcg agtgaaagaa accacggtac
      901 gacttaaagg aaattcccac tctcaacgag ggttagggaa aaaattgaag cgctattgga
      961 aactcctaca aaaagaagaa gcaaagttaa actatgaaaa acgggtttgg cgtgccagtt
     1021 ttaaagatta tttgactgaa agtgagattg ccgatcgact gttagctgct tgtcccaact
     1081 tacgccaagg ctatcagtta taccaagaca ttctttatgc ggtaaagaaa agagatcagt
     1141 cactatttga ggattgttta actagagaca ttagcggtct gccagaaacc tatacaacca
     1201 ctttacgaac gtttaaaaag tttctgccac agatccaaaa tgcgcttcac tacagctatt
     1261 caaacggacc attggaatgc ttaaacaacc acatcaaagt attaaaacgc aatgcgtacg
     1321 gctttcgcaa cttttacaat ttcaaactac gaattatgat tcgtcatggc aaaacatttt
     1381 taaccaaata aacagaacag aaagaggttt tcttctttct gtt
//
LOCUS       DACCCS010000181         1393 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.193, whole genome shotgun sequence.
ACCESSION   DACCCS010000181 DACCCS010000000
VERSION     DACCCS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.193"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            249..>1393
                     /locus_tag="IVK82_002945"
     CDS             249..>1393
                     /locus_tag="IVK82_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HAP6393937.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQK"
BASE COUNT          485 a          227 c          264 g          417 t
ORIGIN      
        1 atgtactcgt gtatagcgaa ggcggattat ggagttcatt gaaacaatcg atttcaatta
       61 tgagaaatga acgaaaaaaa gcataaaata tagaaaaata tagaaggaag ctggacattt
      121 attgttccca gcttcttttt tgtatagacc tatttctatt tttttattat attggatata
      181 tatattcatt gaaaatagag cataacaact ttataatgtt gagtatacca atttttggag
      241 gcgttttaat ggaccgctat ttagatttac tttcagagaa gttccctaca acggaagatg
      301 tcatcacgga aatcatcaat ctcgaagcaa ttttgcagct tccaaaagga acagagttat
      361 ttttgagcga tatacatggc gaatttcctg cttttgatca tattctgcgt atcggttcag
      421 gaaatcttaa agaaaagatc aaagaccttt tctctgaaca aatgacggaa acggagatca
      481 gccaattcac tatttttacg gcttatccag aatatgtgtt gacgactgat tggtacaaaa
      541 aacaagataa atcccagctg atccatcgtt tgatcgatct tttacggttt acgacagtca
      601 aatacactcg atcaaaagtt cgaaaaggat tgccgaaaga atatagctat atcattgagg
      661 aactcttgta tatcgatgat cgtatcaacg ggaaaaaaga ctatgtaaaa aaaatcattg
      721 aacaacttgt cttgatgaag gaagaagaaa aatttttaac aaaacttgcg cagacgatcc
      781 aaaaatctgt catcgatcat ttgcatatcg tgggtgatat ttttgatcga ggaactcaag
      841 cagccaaagt aatggatcga atcatggatt tttcctctgt agatatccag tgggggaatc
      901 atgatatttt gtggattggt gcgtattgcg gctctgaggc atgtttattg acgcttttac
      961 ggattgccgc acgctacaac tatttatttg aattagagaa agaatatgga ttaaatttac
     1021 gtccattatt ttcttttgcc catcaaacct atcagaagaa tccagtattt acccctaaaa
     1081 acaggaaaga tctatctgaa agagagcaag aagaattaga aaagatccat caagcacttt
     1141 ctatcctcca attcaaatca gaacatcagt tgttgaatcg acgacctgaa tttaaaatgg
     1201 acgatcgtaa attgttggaa aaaatcaact atgaggaatc aacgatcgtt ttagaaggtc
     1261 agctctatga gttgaaaggg acttgttttc aaacgatcca gccagatgat cctaaaaaat
     1321 tcttggaaga ggaacaaaaa gtgatcgata gtttgatgta ttcattccag cactcgttaa
     1381 gaatgcaaaa aca
//
LOCUS       DACCCS010000182         1387 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.194, whole genome shotgun sequence.
ACCESSION   DACCCS010000182 DACCCS010000000
VERSION     DACCCS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.194"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1309
                     /locus_tag="IVK82_002946"
     CDS             <1..1309
                     /locus_tag="IVK82_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAP6393938.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          596 a          103 c          233 g          455 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      841 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      901 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      961 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     1021 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     1081 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
     1141 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
     1201 tgaagaatat tatcctattg aaactattgt ttatggaatt aaaaatatta aacaggaatt
     1261 tatcgaaaat ggtagaaaag aaatttataa aaggacttat aaagattgat tatataatat
     1321 atgaaaagct attataaaag acattagtat taaatagttt aaaaaaatga aaaataataa
     1381 aggaagt
//
LOCUS       DACCCS010000183         1359 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.195, whole genome shotgun sequence.
ACCESSION   DACCCS010000183 DACCCS010000000
VERSION     DACCCS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.195"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            253..>1359
                     /locus_tag="IVK82_002947"
     CDS             253..>1359
                     /locus_tag="IVK82_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6393939.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKK"
BASE COUNT          456 a          295 c          251 g          357 t
ORIGIN      
        1 atactaagat tagaggggaa catcattgac gaatgatgtt ctcctctatt ttctattttt
       61 agattatagt gaagagtgaa agaaagttcg aacaaagcct tgaggactac gagtccctat
      121 caaaaaccga acttctttac tgtcatttta gaatcaggtt tacgtatgtt ggggccatga
      181 gcccgtgtat aggaaattgg aatcgaaatg aagcagtaaa gttctttcaa aacttaaaaa
      241 ggaggaaaac aaatgcctta cattcttgca ttagatgttt caatgggaca cagctacagt
      301 gtcctataca aagacgacac ctgcttattt gaagacgaga ttgaacacac tcaaagagga
      361 tttcatgcat tactcgaaga gatccagcac ttgcctgagc gtccattgat tgtttttgaa
      421 gcaacgggga tttattccag accagtcgaa aagttctttc aagatcatca cttttcttac
      481 tgtttgttaa acccgttaga ggcgaaaaaa cagatggaag aaagcacctt acgcagttgg
      541 aaaacagata aatcggatgc ccaccggctc gcacagactc atcttaaaaa tcaacgtcag
      601 ccaaaagaag tacaaagcaa tttttactta gagatgcgcg atttagcccg tttttatcaa
      661 gaaattgaga agaaaatcac tcgtctgcgc atggatttac acaactgcct tcaattgact
      721 tttccagagc ttgaacagtt cttttcaaac aggctgaccc cttacgcatt gacattgatc
      781 cgtctttttc cccatccaga ttttgtattg gcatctactc ggacaaaaat aaaaaataaa
      841 ctgatcaacg aaacacggaa aaaaatctcc gcgaatcgtg ccgagcaaaa ggcagatcaa
      901 atcatccact atgcacagtg tgcctatcca gctgttgaaa aagacagtat ccactgtcaa
      961 aaaacgatct attacgccga acttctccaa gatttactcg aacaaaaaaa agcactggcg
     1021 acacaaatga tcaaaaaagc cgaaggctct ccttgctttt tgttgtacca aacgttccct
     1081 gggattggcg cactcacagc cgctttattg ctcggtgaat taggagatat cacacgattt
     1141 aaaacccata aacaattgaa tgcctttgtc ggaatcgaca tccgacggta tcattcaggt
     1201 aagtatactg gccaagaccg catcaataag cgtggtaatc ccaaagcacg gaaaattatt
     1261 ttcttcacca ttcgaaatat gattcgccag caacgcgcag ctcctaatca tattgtcgat
     1321 tattactaca aattaaaaaa gcaacctatc cctaagaaa
//
LOCUS       DACCCS010000184         1354 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.196, whole genome shotgun sequence.
ACCESSION   DACCCS010000184 DACCCS010000000
VERSION     DACCCS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.196"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            82..>1354
                     /locus_tag="IVK82_002948"
     CDS             82..>1354
                     /locus_tag="IVK82_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP6393940.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLI"
BASE COUNT          460 a          261 c          257 g          376 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atca
//
LOCUS       DACCCS010000185         1347 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.197, whole genome shotgun sequence.
ACCESSION   DACCCS010000185 DACCCS010000000
VERSION     DACCCS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.197"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            69..389
                     /locus_tag="IVK82_002949"
     CDS             69..389
                     /locus_tag="IVK82_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393941.1"
                     /translation="MDKREMQRQKDLLAVEKQSVKVLKNTFADIKEVKIEKSARNEMT
                     GSYRIVILMTNKQDQSIYFSYSFWKERNEIGSYGIVDEKKQKEGNTLNKVKVTYSNGN
                     EESI"
     gene            389..640
                     /locus_tag="IVK82_002950"
     CDS             389..640
                     /locus_tag="IVK82_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393942.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            683..1078
                     /locus_tag="IVK82_002951"
     CDS             683..1078
                     /locus_tag="IVK82_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393943.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            1075..>1347
                     /locus_tag="IVK82_002952"
     CDS             1075..>1347
                     /locus_tag="IVK82_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP6393944.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDA"
BASE COUNT          530 a          186 c          286 g          345 t
ORIGIN      
        1 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
       61 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
      121 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
      181 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
      241 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
      301 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
      361 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
      421 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
      481 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
      541 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
      601 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
      661 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
      721 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
      781 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
      841 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
      901 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
      961 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
     1021 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
     1081 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
     1141 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
     1201 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
     1261 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
     1321 gattggaaag atttggaaac ggatgca
//
LOCUS       DACCCS010000186         1319 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.198, whole genome shotgun sequence.
ACCESSION   DACCCS010000186 DACCCS010000000
VERSION     DACCCS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.198"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(184..>1319)
                     /locus_tag="IVK82_002953"
     CDS             complement(184..>1319)
                     /locus_tag="IVK82_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP6393945.1"
                     /translation="NLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKELASQS
                     SLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQTNYN
                     THYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPNTDIL
                     VRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREVQYFS
                     TTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGTMENF
                     IKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHSIRLK
                     FLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          393 a          248 c          259 g          419 t
ORIGIN      
        1 aattctttgg cacattttat tgaaatatgt atcactaata tccctatcaa ctgattttga
       61 ctaaaaagtt acttcaaaca ttcactgttc aaaaatattg aggaataaat tttgggtgta
      121 aggcattttg agcatacttc tccctggata acttcccagt aaaaaaatcg ttagattgtt
      181 ggattagatc cattgactgg ctcgcctcag gcgagcaaag accctgtaga attcattctg
      241 atacacatga taactagata atttgagata gactcgtcta cccgtttgga ctaatttccc
      301 agcaactttc agaaacttca atcgaataga atgaatggtc attccctttt ggacttcttc
      361 aaagccaatc gttcttaaga agttgactaa gttgtaagct atcaagctga gcatcattcg
      421 gacatgattc tccaaaaagc gaggactgtc tgttttgtca aagtaaaagc cagctttcgc
      481 ttctttaatg aagttctcca ttgtgccacg tttggcatag atagaaaaga tggtatcagg
      541 agaaacattt tctgatagat tcgtcacgat aaattcatgt cgaaagagta gttcgcccgc
      601 ttcacgtgtt gagcgtatac acacgcgacg actttgtgac caggattgtg cttgataagt
      661 ggtcgagaag tactgaactt ctcgttcttc ccacttttga ttatcgccat aaagtactga
      721 tttctcagct atttgaccta gtctacgatt attcttcagt cgaataacat aatgactctt
      781 ttttgattca cacgaatcat acacaccagg tgtagcgaac ccgctgtctc cacgaaccaa
      841 gatgtcagtg tttggtagag agtgattata gtgctctaat aaaggtgtga gaaactcctt
      901 tacgcctttt gatgtatatt gatttcctga acgtagttca gcttttaaga agtcaccagt
      961 caatccgtca aaagctacca atggatggta accataggtt tggtagtgtg tattataatt
     1021 cgtttgttct tggtgaccaa atgtatctga atgggtcgaa tctaaatcaa tgattaattc
     1081 cgtatcatta cggatgaggc gtgctttatc gataagttct tggttcaaag cttgaagttc
     1141 atggatattc tcctcagata gtcgatctaa aaatcgggaa agtgaagatt gagaagcgag
     1201 ttctttccta tctaaaacag ctttaaacac aggatcttgt ctcaggagat tagctgcaga
     1261 atcagctgag taaccagcaa ttaattgcat aatgaactgc tcaagtatgg ataagttgt
//
LOCUS       DACCCS010000187         1317 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.199, whole genome shotgun sequence.
ACCESSION   DACCCS010000187 DACCCS010000000
VERSION     DACCCS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.199"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            100..>1317
                     /locus_tag="IVK82_002954"
     CDS             100..>1317
                     /locus_tag="IVK82_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6393946.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNA"
BASE COUNT          430 a          271 c          240 g          376 t
ORIGIN      
        1 tgacaccagc atctttttta ttttccaaaa catatagaaa agacaccttg aaatccttta
       61 gaattaattt ggcgaaccaa cccaaaagga ggaatcaaga tgtcttacac tcaccttatc
      121 aaagaaacat tagatattct agacttatca gttactttta acgaaaattg tctcacaaaa
      181 gaaaaataca aagggcaaat ctgtcatatt tatcgtggaa acttgattta tacagcccaa
      241 gaatgcatcc actgtaaaca ccagattgct tcagatattg tgcgttgggg tacaacaacc
      301 gtccggttgt taatgaacga tgtttctgaa taccgtacct acctcgaatt gaaaaagcaa
      361 cgcttcaaat gcaaagcttg tcagcggacc tttgtcgcag atacttctgt tgcgaaaaaa
      421 cattgtttca tcagtgagaa agtccgctgg tctgtcgtga ctcgtctgaa aaaaaatacc
      481 tcaatgacag agatcgccgc tcagaaaaat ctatcggtct cttctgttta ccgtattatg
      541 aagcgatttt atcgtccgtt gaatcctttt cggacaccgt tacctaaggt actttgtttt
      601 gatgaattca aatcagttcg gggtgtttca ggtgctatga gtttcatcat gatggatgga
      661 caaacacagc gcttattgga cattgtcgag aaccgacaac tgcctttttt aaaacgttat
      721 tttagtcatt tttcacgaga aattcgtgaa gcggtcgaat ggatcgtgat tgatatgtac
      781 gctccttatg tatcgctcgt taaaaagctg tttccaaaag cacagctgat tattgaccgg
      841 tttcacatcg tccaacatat tggtcgtacc tttcgcaatc atcgaatcaa agaaacgaac
      901 caactattaa aaagcaaaga acaaaaacac tatcaattag gtaagcaatt aaaaagatac
      961 tggaaacttc ttcaaaaaga tgaacgcaag cttgattata ctcgacgcct ttggcgacct
     1021 ggcttcaaag ctcatttaac agagaccgat attgtggatc gcttactgaa agggagccct
     1081 gctttgcgtg taggctatca gttatatcaa gactttcttt acgctgtaaa ggaacgagac
     1141 tacgtatcgt ttgaagaact acttaccaat aatatcatgc ttcccgaagg ctatcagacg
     1201 accttgagaa cgttccagaa atttctgcca caaatcaaaa atgctttaca acaaagttat
     1261 tcaaatggcc cacttgaatg tctgaacaac catatcaaag tgttaaaacg aaatgca
//
LOCUS       DACCCS010000188         1311 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.200, whole genome shotgun sequence.
ACCESSION   DACCCS010000188 DACCCS010000000
VERSION     DACCCS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.200"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            200..1248
                     /locus_tag="IVK82_002955"
                     /pseudo
     CDS             200..1248
                     /locus_tag="IVK82_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          532 a          148 c          254 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DACCCS010000189         1294 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.201, whole genome shotgun sequence.
ACCESSION   DACCCS010000189 DACCCS010000000
VERSION     DACCCS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.201"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1244
                     /locus_tag="IVK82_002956"
     CDS             <1..1244
                     /locus_tag="IVK82_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP6393947.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DACCCS010000190         1292 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.202, whole genome shotgun sequence.
ACCESSION   DACCCS010000190 DACCCS010000000
VERSION     DACCCS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.202"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            141..398
                     /locus_tag="IVK82_002957"
     CDS             141..398
                     /locus_tag="IVK82_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393948.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IVK82_002958"
     CDS             complement(630..1043)
                     /locus_tag="IVK82_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP6393949.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          489 a          167 c          161 g          475 t
ORIGIN      
        1 ttctgtccac gttcatatta ctccaaagtt ttcctcattc caaaagaaaa gcacagaata
       61 cacaaagaag cgtttttatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaaat tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 acaaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatccc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca cacataaaaa taaaaaacat
     1141 ttgtatttta tcaaagtttt attatacttg tcaatttgtg tacatcctaa aatataagct
     1201 atttgtttac ttctgtagaa tatatacttt gttgttagat aagttgcaca tgtttataga
     1261 atctaaataa taaattttta tgtaccgccc ct
//
LOCUS       DACCCS010000191         1287 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.203, whole genome shotgun sequence.
ACCESSION   DACCCS010000191 DACCCS010000000
VERSION     DACCCS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.203"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            316..570
                     /locus_tag="IVK82_002959"
     CDS             316..570
                     /locus_tag="IVK82_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6393950.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="IVK82_002960"
     CDS             571..888
                     /locus_tag="IVK82_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6393951.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          449 a          208 c          255 g          375 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatg
//
LOCUS       DACCCS010000192         1286 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.204, whole genome shotgun sequence.
ACCESSION   DACCCS010000192 DACCCS010000000
VERSION     DACCCS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.204"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            355..1080
                     /locus_tag="IVK82_002961"
     CDS             355..1080
                     /locus_tag="IVK82_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP6393952.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
BASE COUNT          460 a          198 c          258 g          370 t
ORIGIN      
        1 aggttgtgaa aaagctgctc tgcaacaagt aataagaaca gccaagcaaa aacaacttct
       61 tatgttttta acttggatgg acttattacc gcagatgcaa gcttttgcgc accgtttacc
      121 aagcggttgt gaaatcagtg tattgtcttg agtatgaaga aaatttttga ataataataa
      181 ataccttaca gatagagtgg tgactgatta gagacgagga atccgtctct tttttgtatt
      241 tttaagccat cttaaaacag gttttatttt ttgctgtttc tatttctttc tagtatttat
      301 ttatagggga acatatgtta agatttaggg agctgttagt aaaggagtaa agaaatgagc
      361 aaaaaaacaa ccaaggcgat cgtggggatc gtcgctgctg tgattgcagg ggcattcggt
      421 gtttccacca cacaaaaaga ttcagatatc tttcagcaga ttacaactat ttttgattcc
      481 acaccacaag cttctcaagt agcggatgtg ccagaatatg atggaaaaca tcaagaaata
      541 gaaatcaatg ggaatcagcc ggaatttaca aaagaggatc ttagcctttc caaaggaaca
      601 tgggaaagct attcaaatat tgataaattg aacagagtag gacaagccaa tgctatgctt
      661 ggaaaagatt tgttccctac tgaaaagaga gagcctttgt atattgaccc gacaggttgg
      721 aaacaaaaga aattaagtga cgggcaatgg ttatataatc gcagccattt aattggtttc
      781 cagctgactg gtcaaaataa caacattaaa aacttgatga cagggacacg ttcgttgaat
      841 gctccataca tgcttgctca cgaaaatgat attgcggcat atatcaaaga aacaaatcat
      901 catgtacgct accgtgtcac tcctcatttt gaggggaatg aactggtagc caggggagtg
      961 caattggaag cagaaagcat tgaagacaaa aaaattgaat tcaatgtctt tatctacaat
     1021 gttcaagatg gctacacgat caattatgag acaggacaag caacaaaaac aaaaaattag
     1081 tagcagctat tcgaattttt tatgaatatt atgaaaaaat tgtgtaaaaa catacattca
     1141 aagaaatata gcgtatactt tatgaaaaga gtacttttga taaagctaca gaaaaaagga
     1201 aaaaaacata caaatagtgt ttcttaattt attcggaagt ttggaatatt tgtttgtagt
     1261 tttccctttc ttcgagtaaa ataagg
//
LOCUS       DACCCS010000193         1284 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.205, whole genome shotgun sequence.
ACCESSION   DACCCS010000193 DACCCS010000000
VERSION     DACCCS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.205"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>1284
                     /locus_tag="IVK82_002962"
     CDS             <1..>1284
                     /locus_tag="IVK82_002962"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP6393953.1"
                     /translation="GSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGE
                     ALIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPI
                     GRGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGA
                     MDYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSL
                     LLRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTN
                     VISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYRE
                     LEAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIP
                     VDSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEE"
BASE COUNT          401 a          257 c          282 g          344 t
ORIGIN      
        1 ggctcatacg gaatggcgca aaacttagaa tcaaatgatg taggtatcat tatccttggc
       61 gatttcgagt ccattcgtga aggagataaa gtaaaacgaa caggaaagat catggaagtt
      121 ccagttggag aggccttgat tggacgggta gttaatccgc taggtcagcc aattgacgga
      181 ctaggtgaaa tcgttactga caaagctcgt ccagtagaag caatggcacc aggcgttatg
      241 caacgtaaat ctgttaacga accaatgcaa acaggtctaa aagcaatcga cgctcttgta
      301 ccaatcggac gcggccaacg tgaattagtt atcggtgacc gtaaaacagg gaaaacttcc
      361 attgcgatcg atacgatcat caaccaaaaa ggtcaagata tgatctgtat ctatgtagca
      421 atcggacaaa aagattctac agttcgtaca caagttgaaa cattgaaaaa atatggcgca
      481 atggattaca caatcgttgt gaatgccggt gcgtctcaac cagcgccgtt gctttatatc
      541 gcaccatatg ctggtactgc aatgggtgaa gagttcatgt acaacgggaa acatgtcttg
      601 atcatttttg atgatctttc aaaacaagct gttgcttatc gtgaactttc cttgctgctt
      661 cgccgtccac caggtcgtga agcttatcca ggggatgttt tctacttaca ctcacgctta
      721 ttggaacgtg cagcaaaatt aagtgatgat ttaggtggcg gttcaatgac tgccttacca
      781 tttgttgaaa cacaagctgg agatatctct gcttatatcc caacaaacgt tatttcgatc
      841 actgatggac aaatcttctt agaaagcgac ttgttctacg caggtactcg tccagcggtt
      901 gatgccggac tatctgtatc tcgtgtaggg ggttctgcgc aaatcaaagc aatgaaaaaa
      961 gttgctggga ctttacgtct agacttagca agttaccgtg aattagaagc atttacacaa
     1021 ttcggttctg acttggatgc agcaacacaa gcaaaactaa atcgtggacg ccgtacagta
     1081 gaaatcttga aacaaaaatt gcatgcaccg ctagctgttg aaaaacaagt agtgattttg
     1141 tatgctttga cacatggatt cttagatagt atccctgtag acagtatttt agacttcgaa
     1201 catgaattat tcgaatatct ggataccaat cattcagata tctttgaaac aatccgtaca
     1261 acaaaagatc ttccagaaga agaa
//
LOCUS       DACCCS010000194         1276 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.206, whole genome shotgun sequence.
ACCESSION   DACCCS010000194 DACCCS010000000
VERSION     DACCCS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.206"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            35..532
                     /locus_tag="IVK82_002963"
     CDS             35..532
                     /locus_tag="IVK82_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6393954.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="IVK82_002964"
     CDS             complement(569..1252)
                     /locus_tag="IVK82_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393955.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          444 a          216 c          224 g          392 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat tttgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaa
//
LOCUS       DACCCS010000195         1242 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.207, whole genome shotgun sequence.
ACCESSION   DACCCS010000195 DACCCS010000000
VERSION     DACCCS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.207"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            41..1213
                     /locus_tag="IVK82_002965"
     CDS             41..1213
                     /locus_tag="IVK82_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAP6393956.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          461 a          228 c          221 g          332 t
ORIGIN      
        1 atgtttttaa cgacaaaaac atacccagga ggacttttac atgacccaag tacattttac
       61 actgaaaagc gaagagattc aaagcattat tgaatattct gtaaaggatg acgtttctaa
      121 aaatatttta acaacggtat ttaatcaact aatggaaaat caacgaacag aatatattca
      181 agcaaaagaa tatgaacgaa cagaaaaccg acaaagtcaa cgaaatggct attatgagcg
      241 cagctttacg acacgtgtag gcacgctaga attaaaagta cccagaacac gtgatggcca
      301 tttttcaccc acagtgtttg aacgttatca acgaaacgaa aaagccctca tggcttcaat
      361 gttggaaatg tatgtatcag gcgtttcaac tcgtaaagta tcaaaaattg tggaagaact
      421 ttgtggtaaa tccgtctcta agtccttcgt ttctagctta acagaacagc tagaacctat
      481 ggttaacgag tggcagaatc gtttattatc agaaaaaaat tatccttact taatgaccga
      541 tgtactctat ataaaagtac gagaagaaaa tcgagtactc tcaaaaagct gtcatatagc
      601 gattggaata accaaagatg gcgaccgtga aattatcggc ttcatgattc aaagtggcga
      661 aagcgaagag acctggacaa cattttttga atacctaaaa gaacgcggtt tacaaggtac
      721 ggaactcgtt atttctgatg cgcacaaagg attagtctct gccattagaa aatccttcac
      781 caacgtaagt tggcaaagat gccaagttca cttcctaaga aatatcttta ccaccattcc
      841 taaaaaaaat tcaaaatctt tcagagaagc tgttaaagga atttttaagt tcacagatat
      901 taacttagcg cgtgaggcta aaaatcgatt gattcatgat tatatcgatc aaccaaaata
      961 ttcaaaagct tgcgcatcat tggatgatgg attcgaagac gcctttcaat ataccgtaca
     1021 aggaaattcc cacaatcgac taaagagtac caatctaatt gaacgactga atcaagaagt
     1081 acgcagaaga gaaaagatta ttcgcatctt ccccaatcaa acatcagcca atcgcttaat
     1141 tggagccgtt cttatggacc tacatgatga atggatttat tcttcaagaa aatacatcaa
     1201 ttttgataag tagaaatggt aaaaacattg tatagcattt ta
//
LOCUS       DACCCS010000196         1231 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.208, whole genome shotgun sequence.
ACCESSION   DACCCS010000196 DACCCS010000000
VERSION     DACCCS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.208"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>1231
                     /locus_tag="IVK82_002966"
     rRNA            <1..>1231
                     /locus_tag="IVK82_002966"
                     /product="23S ribosomal RNA"
BASE COUNT          323 a          280 c          375 g          253 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc c
//
LOCUS       DACCCS010000197         1229 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.209, whole genome shotgun sequence.
ACCESSION   DACCCS010000197 DACCCS010000000
VERSION     DACCCS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.209"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..803)
                     /locus_tag="IVK82_002967"
     CDS             complement(<1..803)
                     /locus_tag="IVK82_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HAP6393957.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTS"
BASE COUNT          383 a          214 c          241 g          391 t
ORIGIN      
        1 tgtgaggttc gaaaacgatt catgatctgg acacgtaatt gattgaagct gcgattgatg
       61 tgttggacaa tatgaaagcg gtcagttacg atctccgcat ttggaaagac tgttttaagc
      121 aactgacaat agctggcatt catgtccatc actaggtact tgacctttaa acgggccttt
      181 ctaggaaagc gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta
      241 aatagctgat tggttgtccc atcaacacaa ataaaactca tggaaccaga aacagaattc
      301 attgatttga actcatccat acacaacacc gtcggaagga agttcatacg tggctggtaa
      361 tgcttcgttg cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta
      421 gcgattagtt tacgtgattg attttcgtac agttccatga gaatcgcctg cttcagttcc
      481 tttgttagtt gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa
      541 tcatggcaga gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc
      601 aacatctgag tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt
      661 tcacaatagg aaggagagtc atccaatttt cctttaataa actgcgcttg aacgccttta
      721 tattcttttt caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt
      781 aattttctag tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac
      841 taaattttac caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa
      901 aatcgccttc gattttccac caacacaaaa tattatagaa ccaaataaaa atggtgaaga
      961 actttatgag tatgctatga tattgaataa tccagaatat attgaaagtg gcaaagcaga
     1021 aactgttaat tattattatg aacttccgag cgaaatccag aattatcaaa tcgtaatacg
     1081 tgaagcagat ggttattttg ttggcttgcc atttactttg taatctttta tttgaaagtg
     1141 gcttatactt taattgaatt agatgtagga ctgtcaagat aatatcacga gtgattagag
     1201 acaaagaatc tatatcaatt ttacgattt
//
LOCUS       DACCCS010000198         1215 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.210, whole genome shotgun sequence.
ACCESSION   DACCCS010000198 DACCCS010000000
VERSION     DACCCS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.210"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            414..947
                     /locus_tag="IVK82_002968"
     CDS             414..947
                     /locus_tag="IVK82_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP6393958.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACCCS010000199         1193 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.211, whole genome shotgun sequence.
ACCESSION   DACCCS010000199 DACCCS010000000
VERSION     DACCCS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.211"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1076
                     /locus_tag="IVK82_002969"
     CDS             <1..1076
                     /locus_tag="IVK82_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6393959.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACCCS010000200         1153 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.212, whole genome shotgun sequence.
ACCESSION   DACCCS010000200 DACCCS010000000
VERSION     DACCCS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.212"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..969
                     /locus_tag="IVK82_002970"
     CDS             <1..969
                     /locus_tag="IVK82_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6393960.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          390 a          202 c          231 g          330 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aac
//
LOCUS       DACCCS010000201         1145 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.213, whole genome shotgun sequence.
ACCESSION   DACCCS010000201 DACCCS010000000
VERSION     DACCCS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.213"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            4..963
                     /locus_tag="IVK82_002971"
     CDS             4..963
                     /locus_tag="IVK82_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6393961.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          387 a          211 c          229 g          318 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagatcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaa
//
LOCUS       DACCCS010000202         1099 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.214, whole genome shotgun sequence.
ACCESSION   DACCCS010000202 DACCCS010000000
VERSION     DACCCS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.214"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            45..569
                     /locus_tag="IVK82_002972"
     CDS             45..569
                     /locus_tag="IVK82_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393962.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPDGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            597..1004
                     /locus_tag="IVK82_002973"
     CDS             597..1004
                     /locus_tag="IVK82_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393963.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGFGRRQQSQFFNFTENKWEEAVTQDYSKKLELLENLSIGLQVDNAALKKSNEELTEK
                     ADSMAQLNAKLMLNDLTINKKIEAIEKQIGGAS"
     gene            1004..>1099
                     /locus_tag="IVK82_002974"
     CDS             1004..>1099
                     /locus_tag="IVK82_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP6393964.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRQFVPLCITDE"
BASE COUNT          399 a          165 c          238 g          297 t
ORIGIN      
        1 gatggattaa ccaatattac atttgagaaa gtggggacgg tttaatggct gatattgttc
       61 aattaaaaga agacggagtt gctaagtatt tgaaaactca tgccaaagga attgatggtg
      121 tggagggtgt tttagtaaaa gccacaggta atgaaacggt cttaggaacg aaaaatttta
      181 aagacggatt gcaattcaat ggattgcctg tgcaagcagg tatgatcgag cgagctatca
      241 cactagctga tcgaagtgat acaacaaacg taactgatgt gaatggaaaa ataatcagaa
      301 tcggaaacat cgtatttctt acttttaatt ttaaatgtgg cacatggccc gaaggatcag
      361 aaactcgttg gattttaaaa atcccggacg gtttcaaacg tgatcaaggc tatccagcgc
      421 aaaccgcgct ttcactagtt agaaatgcaa gtcagccagc ggatgcacgt gcattcattg
      481 atcaaagtag catcatacaa gccaaatcag gttcaggtag ttcatatatt tcaggtatgt
      541 ggatcactca agatccttgg ccggcttaac agattactaa ttggaggaag agtaaaatga
      601 aaacaattta taaagtattg tatccaatgg gatatgaaga acatcaagta gaagataact
      661 ttccaacatc tttaccattt gtcgaagttc cacctatttt gtttgaaaag aaggaagatg
      721 aaacggatga aggtttcgga agaagacaac agtcgcaatt ctttaacttc actgaaaata
      781 agtgggaaga agcagttaca caagattatt caaaaaaatt agaactacta gaaaatttat
      841 ctataggttt acaggtagat aatgctgcat tgaaaaaatc aaatgaagag ttaacggaaa
      901 aagcagattc gatggcgcag ttgaacgcca aactgatgct taatgattta acaattaata
      961 aaaaaattga agcaatagaa aaacaaattg gaggtgcttc ataatgttta gttttagcga
     1021 tgtgaaaatg atgtatgatt ggggctgttt tactgacgat caagttcgac aattcgttcc
     1081 actatgcatt acagacgaa
//
LOCUS       DACCCS010000203         1089 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.215, whole genome shotgun sequence.
ACCESSION   DACCCS010000203 DACCCS010000000
VERSION     DACCCS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.215"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            1..357
                     /locus_tag="IVK82_002975"
                     /pseudo
     CDS             1..357
                     /locus_tag="IVK82_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          390 a          190 c          149 g          360 t
ORIGIN      
        1 gctgtttatc aagctagctc aaaggaagag gcaaatacct ttttagggag tatgattgag
       61 aagtagcaaa aaacttatcc taaagtgacg cagtcactga taaaaaatca agatttattg
      121 actttttatg agtttccgcc tggtatccgc cgaagcattt actcaactaa tttaatcgaa
      181 tctttcaaga aacaaatcaa gaaatacagc cgcagaaaag agcagtttca aaatgaagaa
      241 tcactagagc gtttcctagt atccatcttt gatacataca atcaaaaatt cttaaataga
      301 agccataaag gcttccaaca agtgacggat acattagctt caatgtttac tgagtaaccc
      361 attattttgc agaaggacga gttatttaca caaaattatt gacgctccct ccaaattact
      421 tcatcaaaac attgatataa tttttattat atcagtattt attcttgata cataaatgag
      481 aaaataaata ctttaaatct attgactaca agtagtcatt aagtcttcat aagatgtaca
      541 atatgacatt aaaaaaataa aagcttacga ccatccgtta aatctgacac taaataatgg
      601 actgctttct tttgtattag caggttacgt tgactatgaa tattcaccag attatttgga
      661 gaaacctctt caacacgaga acaatgatat taattttaga tttgtctacg agtcaaaaag
      721 taaaacatgg ttagttgatc aattaagcac gataaacaca agcttccatg aattacctaa
      781 acctgaaaat gaagttgaga tggtcattaa aatcttctaa aaaagaagcg ttgaagggga
      841 aatattcctt tcaacgctct tttttatatg cttagaaaca taaataaatt gtatactttc
      901 tttttagccc ctattacttt tcacgaaaaa tatctttttt tcgacgctag cgtttctcta
      961 cttctaatac tgtacacagt ttttgtatta ttgaaaaatt actcattatt tagcattaat
     1021 ccacatcgtt ttatcaatcg accttcatac taaactactt taaagtcatc ttccttctcg
     1081 tttttcatt
//
LOCUS       DACCCS010000204         1072 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.216, whole genome shotgun sequence.
ACCESSION   DACCCS010000204 DACCCS010000000
VERSION     DACCCS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.216"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>1072
                     /locus_tag="IVK82_002976"
     CDS             <1..>1072
                     /locus_tag="IVK82_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6393965.1"
                     /translation="IRRVKLELKPGSIDYAEWISRHEKPDLKEQRKEAQGFAYRPLIS
                     ILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDASTDPAIRKCLESYQAKDDRIKV
                     VFRQENGHISLATNSALEMAEGEFIALLDNDDELPPFALYEVAKVLNIHPELDLIYSD
                     EDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYRTSIVKKLGGFRKGYEGSQDYD
                     LVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEAKSYIYDSGVKALTDALSRRNI
                     KGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYEDLKTCVDSIIEKTSYPNYEIII
                     ADNGSTDPKMQELFAEYKHQLKD"
BASE COUNT          351 a          195 c          239 g          287 t
ORIGIN      
        1 atccgccggg tcaaactgga attgaaacca ggatcgatcg actatgcaga gtggatcagt
       61 cgtcacgaga aacctgatct gaaagagcaa aggaaagaag cacaagggtt tgcataccgt
      121 ccattgattt ccatacttat gcctgtctat aacgttgaaa tcaaatggct cgaaaaatgc
      181 attgattcgg tattggatca aacgtatgat cactgggaac tatgtatcag tgatgatgct
      241 tctactgatc cagctatccg aaagtgtctg gaatcctatc aagcaaagga tgatagaatc
      301 aaagttgttt tccgacaaga aaatggacat atcagtctgg caactaattc tgcgttggaa
      361 atggcagaag gagaatttat agcgctctta gataatgatg atgaattacc gccatttgca
      421 ttatatgaag tggcaaaagt gctaaacata catccagaac tggatttgat ctacagtgac
      481 gaagataaaa tcgatgcaga tggcaatcgc tttgatccgc acttcaaagc agactggtct
      541 cccgatacat tgatgggaaa taattatatc tctcacttag gcgtatatcg aacaagtatt
      601 gtgaaaaagc ttggcggttt ccgtaaaggc tacgaaggct ctcaagatta tgatttggtc
      661 cttcgtgtga cagaacaaat ccctgcagat catatttacc atattgaccg agtcttgtat
      721 cattggcgga cgatcccagg atcaaccgca agtaatggtg aagccaaaag ttatatctat
      781 gattctggag tgaaagcatt gacagatgcc ctgtctagaa ggaatatcaa aggaagcgta
      841 cgtcctggca gaatttctgg attttatgag attgcttatg acgtcttgca agaggatttg
      901 gtcagcgtga tcattccaac gaagaatggc tatgaagatc taaaaacttg tgtggattcg
      961 attatcgaaa aaacgtctta tccaaattat gaaatcatta ttgcagataa tggaagtaca
     1021 gatcctaaaa tgcaggaatt atttgcagaa tacaagcatc agctgaaaga cc
//
LOCUS       DACCCS010000205         1062 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.217, whole genome shotgun sequence.
ACCESSION   DACCCS010000205 DACCCS010000000
VERSION     DACCCS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.217"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(36..992)
                     /locus_tag="IVK82_002977"
     CDS             complement(36..992)
                     /locus_tag="IVK82_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393966.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          178 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca ac
//
LOCUS       DACCCS010000206         1056 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.218, whole genome shotgun sequence.
ACCESSION   DACCCS010000206 DACCCS010000000
VERSION     DACCCS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.218"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..397
                     /locus_tag="IVK82_002978"
     CDS             <1..397
                     /locus_tag="IVK82_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393967.1"
                     /translation="HWNAIEGKPTTVKGDKGDPGQAATITIGTVSSGSTASVTNVGTS
                     SAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNAT
                     TTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            397..891
                     /locus_tag="IVK82_002979"
     CDS             397..891
                     /locus_tag="IVK82_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393968.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
BASE COUNT          370 a          170 c          235 g          281 t
ORIGIN      
        1 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
       61 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      121 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      181 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
      241 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
      301 tgctacaact acgacagcag gtttaatgtc agcaaccgat aaagtgaaac ttgatggatt
      361 agcgaatatt acatttgaga aagtggggac ggtttaatgg ctgatattgt tcaattaaaa
      421 gaagacggag ttgctaaata cttaaaaacg catgtagaag ctattgatgg taaagaagca
      481 ttggtgcaga cagatggaga ccaagccatt gcaggacata agaatttttc aggttctgta
      541 acaatcaata ataaacgtgt tttgacgact gatgataaca gatatgaagt ggtaaatctg
      601 gttgtcacta acggtaatac agggacagca aagctttatc gtgaaggaaa aacagtttct
      661 atatattttg tggctttaaa cggaaaaagt agtggcggga atgattcggt tattttaact
      721 gttccagaag gctatcggac accaattagt tttgaacaac tggttgggtc aatagaccgt
      781 tctactttga acagtgctca actatctatt ggcgcagacg gagccattaa atggagaaga
      841 aattcaagtt atggatcagc ttattcattt gttatcactt attcaattta agggaggaaa
      901 tctaatgaaa gtagtttaca aatcaatcaa gccttacgga ttcgagcaaa tcattttgaa
      961 caatcaagaa aatatccctg aaaactgtac agagattaaa ccaccagttc ctaactggag
     1021 accaagattt gattttgata aaaaacagtg ggttga
//
LOCUS       DACCCS010000207         1040 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.219, whole genome shotgun sequence.
ACCESSION   DACCCS010000207 DACCCS010000000
VERSION     DACCCS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.219"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            84..515
                     /gene="ruvX"
                     /locus_tag="IVK82_002980"
     CDS             84..515
                     /gene="ruvX"
                     /locus_tag="IVK82_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP6393969.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            540..845
                     /locus_tag="IVK82_002981"
     CDS             540..845
                     /locus_tag="IVK82_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP6393970.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
BASE COUNT          377 a          179 c          224 g          260 t
ORIGIN      
        1 cgaaatttga ttcgccgtca cgaacgcgat gagatcatgg aagtaattgt caaggactac
       61 ctctcaaatc atggagtgaa tttatgagag taatgggctt ggatgtcggt tctaagacag
      121 taggtgttgc tatcagtgac ccctttggct ggacagctca aggagtagag atcattcgga
      181 tcaatgaagc agaagaagag tttggctttg atcggttgaa ggaattagta gcacaatatg
      241 aagtagaccg gtttgtagtt ggattaccta aaaatatgaa caattccatc ggaccaaggg
      301 cagaagcttc catgtcatac ggagaaaaga tcaaagaact attctccttg ccagttgatt
      361 atcaagatga acgattaacc actgttcagg cagaacgtat gctagtagag caggcaaata
      421 cttcaagagc caaacgaaaa aaagtcatcg ataaattagc agctgtgatg atcttacaaa
      481 attatttaga cggctcaggt aagcaattat tttaataata aaagaaaaga ggtaatctta
      541 tgacagaaca taaccattcc cacgaccatg aaggacacga acacatcaca ttggtggatg
      601 accaaggaaa tgaaacatta tatgaaatcc tactaacagt tgacggacaa gaagaatttg
      661 gacgtaacta tgttctttta tatcctgctg gtgtttcaga agatgaagat gtagaattac
      721 aagcttatgc atacatcgaa aacgaagatg gaacagaagg cgagttagaa caaatcgaaa
      781 cagacgcaga atgggacatg attgaagaag tattcaatac attcatggcg gaagaagaag
      841 aatagaacaa aagaatgaat taataagata taaaaaaagg gattgtgaca aacgtcttgt
      901 cacaatccct ttttccaaat aaacgatgtt caatcagctg cccctcggca ttcgctcgaa
      961 aaacggcgaa atatgaagaa ctattttgcc atttgtttct ctaataatca ggtcaaaacg
     1021 ttgacttcac agtctcttcc
//
LOCUS       DACCCS010000208         1035 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.220, whole genome shotgun sequence.
ACCESSION   DACCCS010000208 DACCCS010000000
VERSION     DACCCS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.220"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..>1035)
                     /locus_tag="IVK82_002982"
     rRNA            complement(<1..>1035)
                     /locus_tag="IVK82_002982"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACCCS010000209         1007 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.221, whole genome shotgun sequence.
ACCESSION   DACCCS010000209 DACCCS010000000
VERSION     DACCCS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.221"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>1007
                     /locus_tag="IVK82_002983"
     CDS             <1..>1007
                     /locus_tag="IVK82_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP6393971.1"
                     /translation="NQDSSAQQEEMKKIIEEYNQYFDTAWSLADIERYNGDINNCLAR
                     KRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEK
                     TKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSS
                     MKSIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIG
                     VYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDARD
                     EIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDELIHAFSN
                     E"
BASE COUNT          362 a          150 c          215 g          280 t
ORIGIN      
        1 aaccaagatt caagtgccca acaagaagaa atgaaaaaaa ttattgagga atataatcag
       61 tattttgata ctgcctggtc attagcagac attgagcgtt ataacggaga catcaataat
      121 tgtttggcac gaaaaagagc ggaattcaaa caatttggga aacaaatcga tttagtaatc
      181 gtagtggatc gtctattgac aggttttgac gcacctacga ttcaaaccct atttgtggat
      241 cgtaatctag agtatgcagg attgattcaa gctttttcac gtacgaaccg tacgtatcct
      301 gaaaagacaa aaggattgat tgtgacgttt cgtaagccag cgacaatgga gaagaatgtc
      361 gaggatgcga caaaactgta ttcagaagca aaagaagaat cgggtttgat ttatccaagt
      421 tatcaggaat ccaaaaaaag attcaagcaa gcgtatggta agttaaagga ggtttcatcc
      481 atgaaatcca ttgatgaaca tacgccactt gaaatccgta tcgagtacgt taaagcattt
      541 caagaattga atcgtgcgta tgaatcgtta gtgacttatg acgattacaa tgatgacatg
      601 gtgcagtctt caactttgaa caatcaaatt caacttttgg aagaacaaat tggcgtgtac
      661 gagaccgtga aggggtcatt aatagaggaa gaaccagaaa aagagggaga agatttttca
      721 acgatcgaat tttatagtga aaataccact aagttgtatg atattgattc tacttatatt
      781 gatcaattac taggaactta tgctgcgaat agttcagatg cacgagatga aattgaaaaa
      841 gctttggcta aattgaataa aacagaagga gtcaaagaag tttaccgaca aattttaaat
      901 gcatttgatg tggggacttt agatagcaat gaagatattt ttgtcattaa acgtcgctat
      961 tttacacaag caagtgatga attgattcat gccttttcca atgagtg
//
LOCUS       DACCCS010000210         1000 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.222, whole genome shotgun sequence.
ACCESSION   DACCCS010000210 DACCCS010000000
VERSION     DACCCS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.222"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            369..1000
                     /locus_tag="IVK82_002984"
                     /pseudo
     CDS             369..1000
                     /locus_tag="IVK82_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DACCCS010000211          998 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.223, whole genome shotgun sequence.
ACCESSION   DACCCS010000211 DACCCS010000000
VERSION     DACCCS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.223"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..968
                     /gene="pepA"
                     /locus_tag="IVK82_002985"
     CDS             <1..968
                     /gene="pepA"
                     /locus_tag="IVK82_002985"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP6393972.1"
                     /translation="ELQLDGLGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENG
                     LFKVVPLGGWNPYVVSAQRFTLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILF
                     DAGFESREEAMSYGVLPGDTIVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQ
                     NETLGHTLIAGANVQEEVGLRGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGE
                     GTLMRIQDPGLIMLPRLREYLLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVI
                     GVVGRYIHTHQTMFSIRDFEAAREMLIQTLKGLDKSTVNTIVYGK"
BASE COUNT          313 a          194 c          228 g          263 t
ORIGIN      
        1 atgagttaca gttagatggt ttgggcggca tttttgggct gcgccaccat gaagaagcgg
       61 atgcacctcg tgtaatggtc gcagcacata tggatgaagt cggattcatg ctgacacaaa
      121 tccaagaaaa cgggcttttt aaagtcgtac ctctaggcgg atggaaccct tacgttgttt
      181 ctgctcaacg ttttacactt aaaacctcta ctggtaagaa ttatccatgt atttcttctt
      241 ccgttccccc acatttatta cgaggaacaa gcggacaaaa atcagtagaa gtaacagata
      301 ttttgttcga tgctgggttt gaatcaagag aagaggcaat gagttacggt gttcttccag
      361 gagacacaat cgttccttac gctgaaacga tcaaaacagc caacgggaag aatatcatca
      421 gcaaatcgtg ggacaaccgc tatggctgta caatggtatt agaagcttta gaagcgttac
      481 aaaatgaaac attaggtcat acattgattg caggcgcgaa tgtccaagaa gaagtaggct
      541 tgcgtggttc taaagcttct gtcaataaat tcaagccaga tctgttcttt gcagtagact
      601 gctctgcagc agatgacaca gtgacgaaaa atggcacatt cggacattta ggagaaggta
      661 cattgatgcg tatccaagat ccaggtttga tcatgcttcc tcgtttgcgt gagtacttgc
      721 tagatatcgc tgaaacaaac aacatccctt atcaatactt tgtttcaaaa ggcggtacag
      781 atgcaggtgc tgctcataca caaaatgaag gaatcccaag tacagtgatt ggcgtagtgg
      841 gacgttatat ccatacgcat caaacgatgt tcagtattcg cgattttgaa gctgcacgag
      901 aaatgttgat tcaaacattg aaaggtttag ataaatcaac agtgaacacg atcgtatacg
      961 ggaaataacg cgatagtgaa actataaaat ctggcaaa
//
LOCUS       DACCCS010000212          943 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.224, whole genome shotgun sequence.
ACCESSION   DACCCS010000212 DACCCS010000000
VERSION     DACCCS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.224"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            2..>943
                     /locus_tag="IVK82_002986"
     rRNA            2..>943
                     /locus_tag="IVK82_002986"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACCCS010000213          890 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.225, whole genome shotgun sequence.
ACCESSION   DACCCS010000213 DACCCS010000000
VERSION     DACCCS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.225"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..888
                     /locus_tag="IVK82_002987"
     CDS             <1..888
                     /locus_tag="IVK82_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP6393973.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACCCS010000214          878 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.226, whole genome shotgun sequence.
ACCESSION   DACCCS010000214 DACCCS010000000
VERSION     DACCCS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.226"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            47..>878
                     /locus_tag="IVK82_002988"
     CDS             47..>878
                     /locus_tag="IVK82_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6393974.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     V"
BASE COUNT          305 a          154 c          167 g          252 t
ORIGIN      
        1 acactcaaaa tagttccgac caaagaatta taaggagtga gtacaaatga cctataccca
       61 tcttacaacg gatgaacttg taataataga gtcttatttc aaaatgaatc aatctgttgc
      121 taaaactgcc cattgcttga atcgttcaag acaaacgatc cataaagtat atctgttctt
      181 caagcaagga aaatcagcct tagaatatta tcaacagtat aagaaaaaca aatcaaactg
      241 tggtagacgt ccgcttgttt tacccgagga acaatcagaa tatattcaaa gaaaggttgt
      301 tcaaggatgg acacccgatg tgattgttgg tcgtgcagcg tttcctatta gttgttctgc
      361 tcgtaccatt tatcgtatgt tcaaaaaggg gctatttgat tcttctgact taccgatgaa
      421 aggcaagcgt aaaccgaatg gacatcaaga aagacgtgga aaacaaactt tccgccgctc
      481 tattcatgaa cgtgaaaagg attatagcca attctcaaat gagtttggtc accttgaagg
      541 tgacactatc gtaggtctga aacataaaag tgctgtaatt accttagttg aacgattatc
      601 aaaagttatc atcacattga aaccgtgtgg tagacaagcg attgatattg aaaaaaaatt
      661 aaatcaatgg tttgaatctg taccgaaaaa cctattcaaa tccatcactt ttgattgtgg
      721 aaaggaattt tcaaattgga aacagatcag taatgtcaat gatattgcca tttatttcgc
      781 tgatccagga acgccgtctc aaagaggcct aaacgagaat tctaacggat tgttacgtag
      841 agatggttta ttgaaatcta tggatttcaa ttcagtag
//
LOCUS       DACCCS010000215          820 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.227, whole genome shotgun sequence.
ACCESSION   DACCCS010000215 DACCCS010000000
VERSION     DACCCS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.227"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>820
                     /locus_tag="IVK82_002989"
     CDS             <1..>820
                     /locus_tag="IVK82_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP6393975.1"
                     /translation="QPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSE
                     ATERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDI
                     SGKEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTR
                     LDHTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKI
                     LKIIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELR"
BASE COUNT          320 a          147 c          161 g          192 t
ORIGIN      
        1 cagccacaaa actctaatta tgtaaaagta ctagaagtaa cccagcaaac gaaacaagat
       61 gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac actaagtttc
      121 cccgtgtcag aagcaacaga aagaatggtg tttcgcttcg cacagcaacc tagctttatc
      181 gaattcagtg cagaagctgc tggagaaaaa ctcatattta tcgattcaaa tgctgtagtc
      241 aaaacagtaa atgattgtta tttgtttgat gggaaagaaa taccagagtt cgctctaacg
      301 gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg ggaattgaca
      361 ccaacaatga aagaattgct ggaaacaatt cgtcctatgg ctgaagtcac aaagcagctt
      421 tctgagaaaa atgatgaatt agaaagagaa aatcagcatc ttcttgagga aaatacaaga
      481 ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga aagtgatgaa
      541 tggacgatca aacatagact aggacgacgt aagaaaaaaa catcgaaaaa aatacaagaa
      601 aaagagcttt ctatatgtat tgatgagaaa atatgggatg cagaaacaaa aatattaaaa
      661 atcatcggat ggggaatcgc taattcggat cgccagccat tgtcctataa gttatcagct
      721 gaccaatcgc catttttcga agcaattcct gtttcacgag atgaagtcaa tcaagcagaa
      781 cagctcgcaa aaggaacaga agcgggattt gaattacgaa
//
LOCUS       DACCCS010000216          809 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.228, whole genome shotgun sequence.
ACCESSION   DACCCS010000216 DACCCS010000000
VERSION     DACCCS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.228"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..195
                     /locus_tag="IVK82_002990"
     CDS             <1..195
                     /locus_tag="IVK82_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP6393976.1"
                     /translation="NDFSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLV
                     NKDRKTKQEIESFLESVIKG"
     gene            217..681
                     /locus_tag="IVK82_002991"
     CDS             217..681
                     /locus_tag="IVK82_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6393977.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
BASE COUNT          286 a          129 c          176 g          218 t
ORIGIN      
        1 aatgactttt ctgattggca atttattgaa gtagaccgtg acaagtacat agatgtagcc
       61 gctgaatgga acatttttgg tatcccaagt tttgtcgtga tccaagacgg aaaagaatta
      121 ggtcgtctag tgaataaaga tcgtaaaaca aaacaggaaa tcgaatcgtt cctagaaagt
      181 gtaataaaag gctgacatct gttaggaggg gtcaagatga atcaagaata taagaacatt
      241 ttagtaggta ttgacggtag tgaacaagca cttgctgctt tcaaaaaagc gttagaagtg
      301 gctcgccgta ataatgggaa agtatatgta gcaaacgtaa tcgatcagca attttataat
      361 tttatgggct atgctcctat tgatcaaaat ctagtcgatc agcaaactga aacagccaag
      421 caaatgatcg aagaatgtaa agaatatgga aaatctgttg attacacaaa tatagaagga
      481 attattgcat acggctctgt tcgtgaatcc atggcagtca aattgccaaa aaaatatgca
      541 atcgatctaa tcatggttgg gcagtctgga ctaaatgcag tcgaacgctt tatgacagga
      601 agtgtagcca gctacgtcat acgtcattca ccttgtgatg tcttgatcgt ttctgacgaa
      661 gaagaacagc caagtaatta agcaagtgga acttgtataa cctttttaat caaacagtaa
      721 agggactgtg acaaaagtct tgtcacagtc ccttgttacg aataaatggt gttccagaag
      781 aagcttcttc ggaaataaag catgaaaaa
//
LOCUS       DACCCS010000217          793 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.229, whole genome shotgun sequence.
ACCESSION   DACCCS010000217 DACCCS010000000
VERSION     DACCCS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.229"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..782
                     /locus_tag="IVK82_002992"
     CDS             <1..782
                     /locus_tag="IVK82_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6393978.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          244 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc ttt
//
LOCUS       DACCCS010000218          775 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.230, whole genome shotgun sequence.
ACCESSION   DACCCS010000218 DACCCS010000000
VERSION     DACCCS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.230"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(249..773)
                     /locus_tag="IVK82_002993"
     CDS             complement(249..773)
                     /locus_tag="IVK82_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393979.1"
                     /translation="MIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          262 a          106 c           87 g          320 t
ORIGIN      
        1 tgacgctccc ttaacaataa atgaagactt ttttattgtt aattgtttat ttttctatta
       61 ttagatatga aaatcgcatt tagataaaat agaaaaatat gaatatattt taaatattaa
      121 gatgagagtc attaacttat gatagtagtc aatttgcttt tcactaatta caaaatatgt
      181 gatataggaa gtttagttat tgttctcata atgccatcct gtttttagat tatctagtaa
      241 tttttttgtt atttaaaatt aggagcagtg ccaattgttt taattttaaa ggcttttttt
      301 atatcttcta aattagaaga tatttgtata tcaatatgga atatattacc ttttttgtat
      361 ttttttatgt tatctttagc taaaacatca aatacatgtc cttgaaattg tatcgaggca
      421 gctggattta gtttaaattg aggaaacttt accacagctg attcaaaaca cttgtcacat
      481 ttttttattt ttttaccttt gtaaaaatat tcgattgaat attctatttg gcacgactca
      541 cttggcagta tatgtatgtc aatatttttc atctgtttca taggtatttc aaaatctaaa
      601 ctattaaaaa catataattc cctgtaatta tttacatcta actctactaa atttatcttt
      661 ggaacaatag atcctccacc cattccacca taataaaaaa aatcttgttc tctatcaaaa
      721 tagcacatat taagctcagt ttgtttatca tcctcaataa ttttataaat caaac
//
LOCUS       DACCCS010000219          752 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.231, whole genome shotgun sequence.
ACCESSION   DACCCS010000219 DACCCS010000000
VERSION     DACCCS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.231"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            362..730
                     /locus_tag="IVK82_002994"
     CDS             362..730
                     /locus_tag="IVK82_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP6393980.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT          243 a          117 c          157 g          235 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt caaaaaatag
       61 cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga gaacaccgtt
      121 taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt ccatataaat
      181 acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa cttatttatt
      241 attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta gattttccca
      301 ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta ggaggaaaca
      361 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
      421 gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      481 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      541 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      601 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      661 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      721 aggtaaataa tagaaataaa gactttgttt tt
//
LOCUS       DACCCS010000220          733 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.232, whole genome shotgun sequence.
ACCESSION   DACCCS010000220 DACCCS010000000
VERSION     DACCCS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.232"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            97..711
                     /locus_tag="IVK82_002995"
     CDS             97..711
                     /locus_tag="IVK82_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6393981.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
BASE COUNT          258 a          130 c          152 g          193 t
ORIGIN      
        1 taccctaaat agacactggt ataacattga aaaatatggc ttcctcgtgt atgattaggg
       61 tgtattttaa atagttgcag aaagtaggta aaagttttga gagttgcata catacgtgtc
      121 tcatcgatcg atcaaaatga acaacgccag attgaagaaa tgaaaaaatt cggtgcagag
      181 agaattttca tcgagaaaca atctggcgca acgattactc atcgacctgt tttccaagaa
      241 gcattagatt acgtccgaga tcaagatatt tttattgtgg aagctattga tcgacttgga
      301 agaaattatg acgagatcat cgcttcagtc aattacttga agaagaaaaa tgtgaagctg
      361 atcattacta gtttaccgat catggctgaa gcaattggca atcctctcct ggataaattc
      421 attaaggatc tgatcatcca aattttggcc atgatcgctg aacaagaacg gactgaatcg
      481 aaacgtcgcc aggcacaagg aattaagatc gcaaaagcaa acggcgtcta caaagggcgg
      541 cccaaattat acagtgcaga cgcaaaagat ccccaacgtc gccttgttta caaaagcatc
      601 gttgaagatc ttaaaaatgg tgttgcgatt gctaagattg ctaaagacta caatgttacg
      661 agacaaaccg tgtatagaat aaaaaaagac agcatggtca atgacaaata gttgatcatg
      721 ctgcttttat tat
//
LOCUS       DACCCS010000221          709 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.233, whole genome shotgun sequence.
ACCESSION   DACCCS010000221 DACCCS010000000
VERSION     DACCCS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.233"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..183
                     /locus_tag="IVK82_002996"
     CDS             <1..183
                     /locus_tag="IVK82_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP6393982.1"
                     /translation="KAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHES
                     LINQYIEGLGSSNETR"
     gene            170..>709
                     /locus_tag="IVK82_002997"
     CDS             170..>709
                     /locus_tag="IVK82_002997"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP6393983.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
BASE COUNT          261 a          114 c          140 g          194 t
ORIGIN      
        1 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgat
       61 gttgcagaac tatctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
      121 atgcacgaat cattaatcaa tcaatatatc gaaggtctag gttcttcaaa tgaaactaga
      181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
      241 taaagcagaa gaaatttatc aagatctcgt aagcttacgt caaatctatg aacaagtacc
      301 tggattaggt aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
      361 ggacaagctt gttagtggct atgatggaat cgttaaaaac tttttagaag ttgtttatgc
      421 gtataaccga atggatgatc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
      481 acatcaaggt ttgctgttgg gaagtgtcac aacagcagtt ccactttcag acgaacaacg
      541 aatacaatta gaaaagaatg ttgcaaaaac aatgaactat caatcagtcg aattaaaaca
      601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gctaaccatc gagtaatcga
      661 cggaagcatt cgcacgcaat tagaaaatat gcgtaatcaa ttaaataga
//
LOCUS       DACCCS010000222          705 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.234, whole genome shotgun sequence.
ACCESSION   DACCCS010000222 DACCCS010000000
VERSION     DACCCS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.234"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..258
                     /locus_tag="IVK82_002998"
     CDS             <1..258
                     /locus_tag="IVK82_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393984.1"
                     /translation="KGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            260..397
                     /locus_tag="IVK82_002999"
     CDS             260..397
                     /locus_tag="IVK82_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP6393985.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            434..>705
                     /locus_tag="IVK82_003000"
     CDS             434..>705
                     /locus_tag="IVK82_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393986.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          279 a           98 c          138 g          190 t
ORIGIN      
        1 aaagggaatg ctgttgatag cgtagatgaa ttggctaata ttaaggcatt atacgaaacg
       61 ctgaaggcag aaaatgatga attgaaacag ctaaattcta aagcaatgct aaacaatgta
      121 gcaatcaagc aagaaaatgt cttattgaaa gaaaagtcag aaagtttagc gcagttgaat
      181 tcaaaaacga tgcttgcttc tgtacaaaat accaaggaaa tcgaagaaat caaaaaacaa
      241 ttacaaggtg ggaagtaaca tgtattcata tgaagatatc aaactaatgt atgactgggg
      301 ctgtttcact aacgaacaag taatggtttt cgttccatta tgcattacag acgaagaagc
      361 agataaaatc attaataaag ataagagcgc atcttaattg atgcgttttt atttaaggta
      421 aaggagttgt cacatgatta atttagggga atggggaatg atagcaggat caataaccgc
      481 tatcgtttct ttgattttat tagtaattag accgattgct gcatctttct cgaaaattac
      541 tgagactctt tcaaaagtaa gccacaattt agatttgctg actaaagatt tagaatcgag
      601 caaatcagat cgattgatga ttcatgaaga actaaagaaa cacgatgaaa gattagatac
      661 acatacagaa aaattggtgg aacacacgca acaaatcaaa acttt
//
LOCUS       DACCCS010000223          677 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.235, whole genome shotgun sequence.
ACCESSION   DACCCS010000223 DACCCS010000000
VERSION     DACCCS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.235"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            50..352
                     /locus_tag="IVK82_003001"
     CDS             50..352
                     /locus_tag="IVK82_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393987.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            349..510
                     /locus_tag="IVK82_003002"
     CDS             349..510
                     /locus_tag="IVK82_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP6393988.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            513..>677
                     /locus_tag="IVK82_003003"
     CDS             513..>677
                     /locus_tag="IVK82_003003"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393989.1"
                     /translation="MDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHDD"
BASE COUNT          258 a          111 c          184 g          124 t
ORIGIN      
        1 gagagcgcac ttgatcaagt actcaaatta ctgaaaggag aatgtcggga tggataagaa
       61 agcggcaatg cagcgaatta tcgaattgac ttattcagaa gattggcaaa atgacaaaga
      121 agctgcttca gaagtgatga ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc
      181 aagaccacga aaaatcgcaa tttggcacgg tgacaaactt ctagtgatag ggacagctga
      241 acagttagca agtctcacag gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg
      301 ctacacagac gctaagaaga gaacgtttag atacgtggag gaatcatcat gacaacagaa
      361 gaagtgattc aaatgcgtat tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca
      421 aaggcagtga tggtcaatga aacggcgaga aaggcaatcg atttgcacat agagaattta
      481 agaagggaaa tccatcgatt ggaggaatga gcgtggataa gaaagcagca atgaaaagaa
      541 ttgctgaatt aaccaagtca gaatcttggc aagaagacaa agaaatagtt gcagaagtcc
      601 aaaagctcgg taaatcaatg tggactgaaa agcccaaacg gagaacgccg agaaaaattg
      661 caatctggca tgatgac
//
LOCUS       DACCCS010000224          666 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.236, whole genome shotgun sequence.
ACCESSION   DACCCS010000224 DACCCS010000000
VERSION     DACCCS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.236"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            55..666
                     /locus_tag="IVK82_003004"
                     /pseudo
     CDS             55..666
                     /locus_tag="IVK82_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          274 a          112 c          125 g          155 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattga
//
LOCUS       DACCCS010000225          656 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.237, whole genome shotgun sequence.
ACCESSION   DACCCS010000225 DACCCS010000000
VERSION     DACCCS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.237"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IVK82_003005"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IVK82_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP6393990.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DACCCS010000226          635 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.238, whole genome shotgun sequence.
ACCESSION   DACCCS010000226 DACCCS010000000
VERSION     DACCCS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.238"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>635
                     /locus_tag="IVK82_003006"
     CDS             <1..>635
                     /locus_tag="IVK82_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP6393991.1"
                     /translation="DLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYP
                     DKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEV
                     SSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQ
                     IGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYA"
BASE COUNT          225 a          101 c          131 g          178 t
ORIGIN      
        1 gatttagcga ttgtagtgga tcgtctatta acaggttttg acgcccctac gattcaaacc
       61 ctatttgtgg atcgtaatct agagtatgca ggattgatcc aagctttttc acgtacgaat
      121 cgtacgtatc ctgataagac aaaaggattg attgtgacgt ttcgtaagcc agcgacaatg
      181 gagaagaatg tcgaggatgc gacaaaactg tattcaaaag caaaagaaga atcaggtttg
      241 atttatccaa gttatcagga atccaaaaaa agattcaaac aagcatatgg taagttaaaa
      301 gaggtttcat ccatggaatc cattgatgaa catacgccac ttgaaatccg tatcgagtac
      361 gtcaaagcat ttcaagaatt gaatcgtgct tatgaatcgt tagtgactta tgacgattac
      421 aatgatgaca tggtgaaatc tccaactttg aacaatcaaa ttcaactttt ggaagaacaa
      481 attggcgtgt acgagaccgt gaagggttca ttaatagagg aagaaccaga aaaagaagga
      541 gaagattttt caacgatcga attttatagt gaaaatacca ctaagttgta tgatattgat
      601 tcaacttata ttgatcaatt actaggaact tatgc
//
LOCUS       DACCCS010000227          632 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.239, whole genome shotgun sequence.
ACCESSION   DACCCS010000227 DACCCS010000000
VERSION     DACCCS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.239"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>632
                     /locus_tag="IVK82_003007"
     CDS             <1..>632
                     /locus_tag="IVK82_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HAP6393992.1"
                     /translation="YTDAKWPEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKV
                     TTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEV
                     DVTVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLPTLPEGTTVTDVTPGGTIDT
                     NTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEVGG"
BASE COUNT          254 a          102 c          148 g          128 t
ORIGIN      
        1 tatacagatg cgaaatggcc agaggaagca actcaaccaa ttacagcaga tgatacagaa
       61 tttgtatcaa gtgcaacaaa actggatgat aaatctgacg ctgacaaata taatcctgaa
      121 ggtcaaaaag tgactacaga attgaataag gaaccagacg catttgaggg aattaagaac
      181 aaagaagatt taccaaaaga tactaagtat acttggaaag aaaaagtaga tgttagcgca
      241 gctggaaata aaaaagggac tgttgtagtg acatatccag atggatcatc tgatgaagtt
      301 gaagtagatg ttacagtgac agacaatcgt tctgacgccg ataaatatga gccaacagta
      361 gaaggcgaaa aagtagaagt tggaggcaca gtagatctaa cagataatgt aaccaaccta
      421 ccaacattac cagaaggaac aacagtaaca gatgtcactc ctggtggtac gattgatacc
      481 aacacaccag gtaattacga aggggttatc gaagtaacgt atccagatgg tacgaaagat
      541 acagtgaaag ttccagtaga agtgacagac aatcgttccg atgccgataa atatgaacca
      601 acagtagaag gcgaaaaagt agaagttggg gg
//
LOCUS       DACCCS010000228          626 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.240, whole genome shotgun sequence.
ACCESSION   DACCCS010000228 DACCCS010000000
VERSION     DACCCS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.240"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..339
                     /locus_tag="IVK82_003008"
     CDS             <1..339
                     /locus_tag="IVK82_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP6393993.1"
                     /translation="YFHFVENHLALVGERTGLTFKIGQKVRVKVVKADPDERAVDFEL
                     VSAEEVTALERPKTRNGKSGKREGKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFY
                     KGVKKKKKKK"
     gene            361..>626
                     /gene="smpB"
                     /locus_tag="IVK82_003009"
     CDS             361..>626
                     /gene="smpB"
                     /locus_tag="IVK82_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP6393994.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHK"
BASE COUNT          250 a          100 c          144 g          132 t
ORIGIN      
        1 tacttccatt ttgttgaaaa tcatttagct cttgtaggtg aaagaactgg tctgactttt
       61 aaaatcggac agaaagtgcg agtaaaagta gtcaaagctg atccagatga acgagcagta
      121 gattttgaac ttgtttccgc agaagaggtt acagcgttgg aacggccaaa aacacgaaat
      181 ggaaaatcag gaaaacgcga aggaaaacaa gcaggaaaac gcccagaaag aaatggcaag
      241 aagaacaaac gatatagcga tagtaagcaa acgttcggta agaaaaaagg gaagaagcca
      301 ttttacaaag gtgttaaaaa gaagaaaaag aaaaaataaa atgagttgga ggaaacagct
      361 atgccaaaag gcgagggaaa attaattgca caaaacaaga aagctcgcca tgattattcg
      421 atcatcgaca cgatggaagc agggatggtc ttgcaaggaa ccgagatcaa gtcgatacga
      481 aacagccgga tcaatctaaa agatggattt attcgcgtcc gcaacggaga agctttcttg
      541 cataatgttc atatcagtcc ttatgaacaa ggaaatattt ttaatcatga tccgttgcgc
      601 acgagaaagt tattattaca caaaaa
//
LOCUS       DACCCS010000229          615 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.241, whole genome shotgun sequence.
ACCESSION   DACCCS010000229 DACCCS010000000
VERSION     DACCCS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.241"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            75..>615
                     /locus_tag="IVK82_003010"
     CDS             75..>615
                     /locus_tag="IVK82_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6393995.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQ"
BASE COUNT          215 a           78 c          138 g          184 t
ORIGIN      
        1 ggagctttga ttattgcaac tgttgatgat ccaaaacaga atgttttcac tcttgagaaa
       61 gttggggtga tttgatggtt aaagtaaaga gaatgatgga gactgatgaa tctggtgtta
      121 aacgtcaatt tatgccaatt acacatgttt ctgcagtttt aggcttagaa aaaatgatat
      181 caggacaatc aaaagttctt tcggttaatg gtaaatatgg tgcagtcatt ttaacgaaag
      241 ctgatttagg attagagaat gcaattactg aactacctta tgcaagcgag acaagcgatg
      301 gtattcttac tgctgaaatg tttcaaaaga tcgtaaatgg agaaggtgga acatatattt
      361 tgccgattgc tactcctgaa caattaggag gtataaaagt tggggaactc ttagaagtta
      421 ctgaagaagg tgttttatct gcaactaagc aaacagactt caatttctct gaagaattaa
      481 agggaaaatt agaatctcta aaaatattaa aagcaggtgc aaatatatca attgcagaag
      541 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgttaatcag tcatatgtag
      601 atcaaaaatt ccaag
//
LOCUS       DACCCS010000230          601 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.242, whole genome shotgun sequence.
ACCESSION   DACCCS010000230 DACCCS010000000
VERSION     DACCCS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.242"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>601
                     /locus_tag="IVK82_003011"
     CDS             <1..>601
                     /locus_tag="IVK82_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HAP6393996.1"
                     /translation="FGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDG
                     QQGLEVMMKDGTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAI
                     GSPLGSEYANSVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIE
                     GQVIGINSSKIASTSESTSNVSVEGMGFAIPSNDVVNII"
BASE COUNT          200 a          104 c          146 g          151 t
ORIGIN      
        1 tattcgggca gcaagaagaa agtagcagct cagatgatag taatttggaa gcttatagtg
       61 aaggaagcgg cgtaatctac aaaaaagacg ggaatactgc ttatgtagtc acaaacaatc
      121 acgtagtaga tggacagcaa ggtttggaag tcatgatgaa agacggtacg aaggtaaaag
      181 ctgaattagt tggaacagat gcctattcag atttagcggt gttgaagatc aattcagaca
      241 aagtcgaaac ggttgcctct tttggggact caagtgcttt gaaggttggt gaacctgcga
      301 ttgcgattgg ttccccatta ggatctgaat atgcgaattc tgtcacttca ggaatcattt
      361 cttccttgaa ccgacaagta acaagtacga atgaatcgaa cgaaacagtc aatatcaatg
      421 cgatccaaac agatgcagcg atcaacccgg gaaattctgg tggtccttta gtgaatatcg
      481 aaggacaagt tatcggtatc aactcaagta agatcgcaag tacttctgaa tcgacttcta
      541 atgtaagcgt agaaggaatg ggatttgcca tcccaagtaa cgatgtagtg aatatcatca
      601 a
//
LOCUS       DACCCS010000231          600 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.243, whole genome shotgun sequence.
ACCESSION   DACCCS010000231 DACCCS010000000
VERSION     DACCCS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.243"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>600
                     /locus_tag="IVK82_003012"
     CDS             <1..>600
                     /locus_tag="IVK82_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6393997.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESF"
BASE COUNT          169 a          129 c          137 g          165 t
ORIGIN      
        1 atgactaaat catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt
       61 tatgccgcta tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag
      121 gaagccattc atatcgtagt tggcattcgc ccggacggtt caaaggaagt gttgagctat
      181 gcgattgcac caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa
      241 cgtggcgtga aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc
      301 gtgcagcggt tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat
      361 atcagtcaca aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg
      421 tatcaagcgt catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg
      481 aaaaccagtt atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc
      541 tatgatttcc cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt
//
LOCUS       DACCCS010000232          598 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.244, whole genome shotgun sequence.
ACCESSION   DACCCS010000232 DACCCS010000000
VERSION     DACCCS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.244"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..564
                     /locus_tag="IVK82_003013"
     CDS             <1..564
                     /locus_tag="IVK82_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP6393998.1"
                     /translation="MVIIADDKGTENTVERKWNVARIKDEQGQVVAWNFFHISDHLTI
                     EGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKTCVPHPDSDHLSITE
                     TEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTLRGVASYGMICSARE
                     LHLPNAPEKKGILVLPEDAVVGEVFPK"
BASE COUNT          195 a          106 c          146 g          151 t
ORIGIN      
        1 atggttatca tagccgacga taaaggaaca gaaaacactg tcgaacgtaa atggaatgtt
       61 gctcggatca aagatgaaca aggtcaagtt gttgcttgga actttttcca tatctccgat
      121 catctaacaa ttgaagggaa cggccaagta agcttaacag atgaagaatt agcgaaattg
      181 aatcgattga ttaaagaagc aggattcgaa gaacagcttg aagctgatca tgaaccgaag
      241 atcgttgtcg ggtatgtaaa aacatgcgtc ccgcatcctg actctgacca tttatcgatt
      301 actgagacag aagtggacaa tgggacagta ttgcaaattg tttgcggtgc accaaatatc
      361 gaagctggac aaaaagttgt cgttgcaaaa ccaggagcca tgatgccaga tggaatgatg
      421 atctggccag gaactttgcg aggggtagcc agctatggaa tgatttgttc tgctcgtgaa
      481 ttgcatttgc caaatgctcc agaaaaaaaa ggaatcttag tattgcctga ggatgccgta
      541 gtcggagaag tatttccaaa ataaagaggt tgtgacaagt ctttgtcaca acctcttt
//
LOCUS       DACCCS010000233          593 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.245, whole genome shotgun sequence.
ACCESSION   DACCCS010000233 DACCCS010000000
VERSION     DACCCS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.245"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..580
                     /locus_tag="IVK82_003014"
     CDS             <1..580
                     /locus_tag="IVK82_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6393999.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DACCCS010000234          585 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.246, whole genome shotgun sequence.
ACCESSION   DACCCS010000234 DACCCS010000000
VERSION     DACCCS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.246"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(128..>585)
                     /locus_tag="IVK82_003015"
     CDS             complement(128..>585)
                     /locus_tag="IVK82_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP6394000.1"
                     /translation="LDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRG
                     KEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRL
                     ELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          168 a          133 c          108 g          176 t
ORIGIN      
        1 gaatggcaac actttttttg acaaaaatta taaggtgttg tgaatgctgc ggtgttatgg
       61 ctacgattgc ccttgacaat gagcatcctc ggatctgatt ttctccggca gaagcacaat
      121 cattcaatta cgctgcctct ccagaggtat cagatccgcg ctcattatca aggattcgct
      181 tcgccaatct ctgttgtcga atttggatcg gtgtttcata cgccaacgtg ccatgtaagc
      241 gtagatagtt ccaccaatgc acataatcaa acaattctag acgtagctgt gccagtgtct
      301 caaattggta ttgatgcacg aattctactt tgacagattt ataggttgat tccacaacgg
      361 cattatcata cggacaaccc ttcctactca atgaacgagt aattccaaaa ccattcagaa
      421 tctcatgaat ggtttggtta tcaaattcct ttccccggtc tgtatgaaaa agcttgacgt
      481 ctgttaaaga atacggtatc cgtccaaagg cttcttttac caatgaggca tctttcttct
      541 caccacagga ataaccaata atttctcgat taaacaaatc aagta
//
LOCUS       DACCCS010000235          563 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.247, whole genome shotgun sequence.
ACCESSION   DACCCS010000235 DACCCS010000000
VERSION     DACCCS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.247"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..267
                     /locus_tag="IVK82_003016"
     CDS             <1..267
                     /locus_tag="IVK82_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6394001.1"
                     /translation="SHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEAEEFVYPFRGY
                     VKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            344..>563
                     /locus_tag="IVK82_003017"
     CDS             344..>563
                     /locus_tag="IVK82_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP6394002.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAIL"
BASE COUNT          196 a           96 c          136 g          135 t
ORIGIN      
        1 agtcacggta tcggcggttt aatgctagat atgccgacaa agaaccctaa aacacgcaag
       61 aaatcaaaat tcaaagtcgg tgacatggtc cgctgtgaag cagaagagtt cgtttatccg
      121 ttcagaggat acgttaaaaa gatactgtca aactcagcaa tcattcgtat tgaaaataca
      181 atgaagtgtg atcgttcgac tgcaaaacac aagcattatt tagctgtagc gagattagtt
      241 gacatggaag taatcaagag caaataaaaa aagccggatc gctccgactg attcaataaa
      301 tccaacacat ttattatatc acataaagga gcggtttgac ttgatgcaat tgttacgaga
      361 ggtagatttc aaacagacaa gatgtaatgc gagagatgtg ctgaagaact ttcggcgttt
      421 ggagcggatg gcaggtcgct ctttgataga tattaagtcg ccgattataa cggatatgcc
      481 gaaggcaccg aagcatggca ataaggcaga agacgcgatt attcagatga tggatataga
      541 aacggagaga gatgcgattc tag
//
LOCUS       DACCCS010000236          546 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.248, whole genome shotgun sequence.
ACCESSION   DACCCS010000236 DACCCS010000000
VERSION     DACCCS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.248"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(271..534)
                     /locus_tag="IVK82_003018"
     CDS             complement(271..534)
                     /locus_tag="IVK82_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAP6394003.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          213 a           97 c           75 g          161 t
ORIGIN      
        1 actctcctga attttttcat gttgaaaaca tcactaatca aaggcaaatt ataatttaga
       61 aatagaatga aaacactaaa tgaaaataga acattagaaa aaatagaatc aaaaaaaccc
      121 aagactagtg ccgaaataag ggaaacaatc gaagctacaa aaaataatga tttcgttgaa
      181 caggaccaat ttatttgttt ccgatatttc tcaaaaagaa ctttctttgg gaaagttaaa
      241 ggcgactgta aagtcgcctt tagtcctttt ttaccaagct gctgcatatt tagctccata
      301 ttttttaatt aatgatttgg ctgtagcaac aataccagct gtaattaatc cgcctccaac
      361 aactgcagca acaatagaaa tgattgtagc aaccgtgcta ccagtattaa ttatgtcaat
      421 aactttttta gctgcataag aagaaattcc aatgttagca cttagcattg gtacaccaac
      481 aaagaatccc gcaaaaccta aaattggtaa aactaataat aaattctttt tcataataat
      541 tcctct
//
LOCUS       DACCCS010000237          544 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.249, whole genome shotgun sequence.
ACCESSION   DACCCS010000237 DACCCS010000000
VERSION     DACCCS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.249"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..297
                     /locus_tag="IVK82_003019"
     CDS             <1..297
                     /locus_tag="IVK82_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP6394004.1"
                     /translation="ALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLR
                     KIKQNLFWAFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            317..>544
                     /locus_tag="IVK82_003020"
     CDS             317..>544
                     /locus_tag="IVK82_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP6394005.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHM"
BASE COUNT          191 a           93 c          132 g          128 t
ORIGIN      
        1 gcattagctg atgtcggtat ggcaatggga agtggaacgg acattgcgat ggaaacagcc
       61 gatgtgacgc tgatgaatag cagtttggca tccattgcac aaacaatcga gttgtctagg
      121 ttgacattga gaaaaatcaa acaaaatctt ttctgggctt ttgtctataa tacgattggt
      181 attccttttg ccgcactagg ctttttgaat ccaatcattg caggaggagc gatggcgttt
      241 agttccgtca gtgtattgtt gaactccctg agcttaaatc gacacatgaa aaagtaagag
      301 aagggttgga gtaaaaatga atgggaacga tatggagaaa aaacatgagc ataaaaaaga
      361 agtagtaaac aaagaaagta cagaatctca tgaaaaacag atggagatga aacatgacca
      421 tagtcagatg gatcattcca tgcacatggg acataaccat ggagatatag caaacagcaa
      481 acaaatggac cattcgatgc acatggatca cgaacacggc ggcatggatc attccatgca
      541 catg
//
LOCUS       DACCCS010000238          541 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.250, whole genome shotgun sequence.
ACCESSION   DACCCS010000238 DACCCS010000000
VERSION     DACCCS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.250"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..491)
                     /locus_tag="IVK82_003021"
     CDS             complement(<1..491)
                     /locus_tag="IVK82_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HAP6394006.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHD"
BASE COUNT          138 a          107 c           93 g          203 t
ORIGIN      
        1 tcatcgtgag gaacttgcat aacggcaatc gtagaagcgt gtgtacgctc atagtcatcg
       61 atcaattgct gtgtcaatgg tacttcgtct tccattatat catcgcctag cataacgaca
      121 aatggttcat ttccaataaa ggctcttgct tgaagaacgg catgtcctaa accctttgga
      181 tgagattgac ggatgaaatg caagttgaca tctgtcgttt cttctactaa tttcaataat
      241 tcggtctttc ctttttcact taggtttgtt tctaattcaa tatttgaatc aaagtgatct
      301 tcgattggtc gtttagcttt tcctgtaacg atcaagatgt cttcgatacc tgatttcaac
      361 gcttcttcaa cgatgaactg gattgttggt ttatcaacaa ttggcaacat ttcctttgcc
      421 atagcttttg ttgctggtaa aaatcttgtt cctaatcctg ctgctggaat aactgctttt
      481 ttaactttca tatacattcg ctccttcaaa aaattgttaa tgagaattca ttttcttttt
      541 t
//
LOCUS       DACCCS010000239          528 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.251, whole genome shotgun sequence.
ACCESSION   DACCCS010000239 DACCCS010000000
VERSION     DACCCS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.251"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(5..90)
                     /locus_tag="IVK82_003022"
     tRNA            complement(5..90)
                     /locus_tag="IVK82_003022"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IVK82_003023"
     tRNA            complement(107..178)
                     /locus_tag="IVK82_003023"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IVK82_003024"
     tRNA            complement(190..262)
                     /locus_tag="IVK82_003024"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IVK82_003025"
     tRNA            complement(280..361)
                     /locus_tag="IVK82_003025"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IVK82_003026"
     tRNA            complement(369..441)
                     /locus_tag="IVK82_003026"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IVK82_003027"
     tRNA            complement(445..517)
                     /locus_tag="IVK82_003027"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACCCS010000240          527 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.252, whole genome shotgun sequence.
ACCESSION   DACCCS010000240 DACCCS010000000
VERSION     DACCCS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.252"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            258..527
                     /locus_tag="IVK82_003028"
                     /pseudo
     CDS             258..527
                     /locus_tag="IVK82_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          181 a           94 c           76 g          176 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataac
//
LOCUS       DACCCS010000241          512 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.253, whole genome shotgun sequence.
ACCESSION   DACCCS010000241 DACCCS010000000
VERSION     DACCCS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.253"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(136..426)
                     /locus_tag="IVK82_003029"
     CDS             complement(136..426)
                     /locus_tag="IVK82_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6394007.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
BASE COUNT          217 a           81 c           73 g          141 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa at
//
LOCUS       DACCCS010000242          510 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.254, whole genome shotgun sequence.
ACCESSION   DACCCS010000242 DACCCS010000000
VERSION     DACCCS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.254"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..508
                     /locus_tag="IVK82_003030"
     CDS             <1..508
                     /locus_tag="IVK82_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HAP6394008.1"
                     /translation="DLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFS
                     RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLK
                     VEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELG
                     CANASEAV"
BASE COUNT          155 a           90 c          110 g          155 t
ORIGIN      
        1 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
       61 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      121 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      181 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      241 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      301 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      361 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      421 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      481 atgtgctaac gctagcgagg cagtctaact
//
LOCUS       DACCCS010000243          509 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.255, whole genome shotgun sequence.
ACCESSION   DACCCS010000243 DACCCS010000000
VERSION     DACCCS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.255"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..132)
                     /locus_tag="IVK82_003031"
     CDS             complement(<1..132)
                     /locus_tag="IVK82_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6394009.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DACCCS010000244          507 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.257, whole genome shotgun sequence.
ACCESSION   DACCCS010000244 DACCCS010000000
VERSION     DACCCS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.257"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..>507)
                     /locus_tag="IVK82_003032"
     CDS             complement(<1..>507)
                     /locus_tag="IVK82_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP6394010.1"
                     /translation="KSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNASGLS
                     TALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDVVIF
                     DMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYNGAE
                     HSKDAGYYY"
BASE COUNT          126 a          112 c          108 g          161 t
ORIGIN      
        1 gtagtaataa ccagcatctt tgctatgttc tgctccgttg tacacaacac ctaagactcg
       61 tgcttgtacc atctccagca gttcttttgc tttagtcaag gattctttac gtgacatatt
      121 ttcacgtacg accaataaag tgccatccgt ttttaaagac atgatctgtg catctgttac
      181 ggctactact ggtggcatat cgaatatcac cacgtcaaat aactgtcttg cttcggatag
      241 gatctgatcc attcttggcg agcttaacaa ttctgatggg ttgggtggct taggtccact
      301 aggcaaaatc gacaagttct cgactggcgt tcgttggatc acatctgcta cacttcctga
      361 tgaactaagt gctgtactta atccactggc attgttcagc tggaatgtct tatagacgac
      421 gggtttacgt aaatctgcat cgactagtag aactcgttga cctgatttag caaaaacgac
      481 tgcaatgttt gctgcagtgg tggattt
//
LOCUS       DACCCS010000245          504 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.258, whole genome shotgun sequence.
ACCESSION   DACCCS010000245 DACCCS010000000
VERSION     DACCCS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.258"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT          226 a           70 c           81 g          127 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
      241 aaaaaagtta ttgacatgtt actaacgaca tgataagata actgagtcgt taaaaaagaa
      301 ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
      361 acgataagtt atgataaatg agttgccgaa agatttcgaa aaacgaaatc gaaaaaaact
      421 tttaaaaaag agttgacaat cgcttgtctc tctgatatga tataaaagtt gctacggcag
      481 ccaaaaatga atcgggaaac actc
//
LOCUS       DACCCS010000246          489 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.259, whole genome shotgun sequence.
ACCESSION   DACCCS010000246 DACCCS010000000
VERSION     DACCCS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.259"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            107..331
                     /locus_tag="IVK82_003033"
     CDS             107..331
                     /locus_tag="IVK82_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP6394011.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT          219 a           61 c           97 g          112 t
ORIGIN      
        1 ttccgtgacg gctcttttta ctatattaaa atcatagaaa tgtctaatca aaaaaatata
       61 aaaattattt tgcgtacata tgattaaaga aaggagagtg tgacacatgg aaacagaatg
      121 gcaaagacgt gaaagaaaaa aacagaaaaa agaagagatg ttacgattat gtcgattatt
      181 agatgtaaaa gactgggaca aaaacgagca aattgcacaa aaagtacgat caatcatcca
      241 taaagaggaa aaagttaaaa ataaagacac gcgtagaact gaaaatgaaa agagaaaagt
      301 acaaaagaaa agagaaaaat caaaaagcta gaaagaaaaa gaggctgaga cagaagcgtt
      361 taactccaag aaataagctg gaattcacaa aaatttgaaa agcaattttc gtgaatttca
      421 gcttatttcc gaaggagttg cttctgcttt cgccgtttat acgtgtttag agcgtgagac
      481 aaaagtgtt
//
LOCUS       DACCCS010000247          488 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.260, whole genome shotgun sequence.
ACCESSION   DACCCS010000247 DACCCS010000000
VERSION     DACCCS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.260"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..414)
                     /locus_tag="IVK82_003034"
     CDS             complement(<1..414)
                     /locus_tag="IVK82_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6394012.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          185 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaaacggc tttccaaatg atgaaatacc taaatctaac ttattaagaa agttgaaatt
       61 tttatattca ggaaaccatt tattaatttc atgtgcagca cgtaatgcat tccctggagt
      121 tgtcaaagag aaaattaatt ctgctaagct tattattgaa aatactgata acttataatt
      181 atatcgtcca ttgacaatca aatataaact aactatactt gttagaacaa aaaaacaggc
      241 gttgacttgt tcttgattag ttaaaaatat caataaagga atacttaccc aatatatttt
      301 tctatttatt tcttctcctc ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa
      361 tgctactggc cactgatagt tcaaggtggt tgctctccaa ccggtttccc ccattactgt
      421 caatggtatt aaacagtata tagagcaaac caataataaa tttttaacat ttattttctc
      481 aaaaacat
//
LOCUS       DACCCS010000248          462 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.262, whole genome shotgun sequence.
ACCESSION   DACCCS010000248 DACCCS010000000
VERSION     DACCCS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.262"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..430)
                     /locus_tag="IVK82_003035"
     CDS             complement(<1..430)
                     /locus_tag="IVK82_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP6394013.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DACCCS010000249          449 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.263, whole genome shotgun sequence.
ACCESSION   DACCCS010000249 DACCCS010000000
VERSION     DACCCS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.263"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT          143 a           90 c           67 g          149 t
ORIGIN      
        1 acactcctct taatatacag tcctattgta taaagtgtta ctatatacat agtaactgta
       61 tacataaaaa aagtcaagta ttagaaaaaa gttcaaaaga tcaagtgtgc gcttcgctcc
      121 cactcccctc gacgttttag tagcctttcc ctcacttcgt tcagtccaag ccaactaaaa
      181 agttttcggg atacattctc cttctcttcc taataatcaa ttaaaatttt aatctttata
      241 tttctgctaa tcactcagga aacagtacag aaaaacaaac acgtatcata gatataaatg
      301 taatggcata gtgcgggttt tattttcagc ctgtatcata gctaaacaaa tcgagttgtg
      361 tgtccgtttt tgggcgttct gctagcttgt ttaaagtctc ttgaatgaat gtatgttcta
      421 agtcaaaaaa ctctgacagc gcctttata
//
LOCUS       DACCCS010000250          439 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.264, whole genome shotgun sequence.
ACCESSION   DACCCS010000250 DACCCS010000000
VERSION     DACCCS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.264"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>439
                     /locus_tag="IVK82_003036"
     CDS             <1..>439
                     /locus_tag="IVK82_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP6394014.1"
                     /translation="SLEIVDGLALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLM
                     YRKEQNRLMNAVYDHFTKDESKDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVS
                     LVLQQQIIEKDIPLLNQLLDQPIQPVIIKSYRHYIDLQRFKGTL"
BASE COUNT          159 a           88 c           85 g          107 t
ORIGIN      
        1 cctcattaga aatcgtcgat gggctagctt tgcagaaaaa agaagtggaa ctattcacat
       61 ccgtccatta tggcgagcgg acatatccaa gaaaaaaaca agcaaaagaa aaaatgtttg
      121 gtaaaacact gatgtatcga aaagaacaga acagactgat gaatgcggtc tacgaccatt
      181 tcacaaaaga tgaatcaaaa gatttgatga tcgaagcagc tacaggaatg ggtaaaacaa
      241 tcggttatct gctgccattg agttatttgg ctacacctga aaaacccgct gtcatcagta
      301 ccgtttcgct agttttgcag cagcagatca tagaaaaaga cattccattg ctgaatcaat
      361 tgctagatca gcccatacaa cctgtaatta tcaaaagcta tcgccactat attgatttac
      421 agcgttttaa aggcacact
//
LOCUS       DACCCS010000251          439 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.265, whole genome shotgun sequence.
ACCESSION   DACCCS010000251 DACCCS010000000
VERSION     DACCCS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.265"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            1..379
                     /locus_tag="IVK82_003037"
                     /pseudo
     CDS             1..379
                     /locus_tag="IVK82_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          124 a           96 c           81 g          138 t
ORIGIN      
        1 cgtttcttgg tcaaccgttt cgcgaacact cgcttctctg ctccccatga caaacgtgaa
       61 aataaattgg ttgcctcgct gttcattggt agatgaattt cgttcattga aaaatcaagt
      121 cggtccctat tctttttctt gtatggatgg cgatactggg aaattactgg atattcttcc
      181 ttcacgtaag aagaaagatc tcatttctta ttttatgcaa tttgaacgac gtgcacgttt
      241 aaacgtaaaa atactcgtaa cagatatgaa cgcctcttat gctttgctga taaaagagtg
      301 ctttcctcat gccaaactcg ttgtggatcg ctttcacatc gtcaagcacc tcattcgcag
      361 ttttaaggat atttgactat gagtcatgaa aagcttcgac cgaaacgatc caatacaagc
      421 caaacactat cgacaagtg
//
LOCUS       DACCCS010000252          435 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.266, whole genome shotgun sequence.
ACCESSION   DACCCS010000252 DACCCS010000000
VERSION     DACCCS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.266"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            273..>435
                     /locus_tag="IVK82_003038"
     CDS             273..>435
                     /locus_tag="IVK82_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6394015.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQE"
BASE COUNT          171 a           53 c           86 g          125 t
ORIGIN      
        1 gcagaaataa tagaaaacag tcgagaggag aatggtaaaa tcatcatttc agtagaaaaa
       61 ttcaatgaat tgttctcaga aacatttgaa atagatacag attattatca accacaagaa
      121 gtttttcgat tgtttagaga tcagattatt ggtattagtc aaactacaaa atgaaagctt
      181 aataaagcaa gcgttgaaag aattattcct caacgcttgc tttatttgat gggcgcttca
      241 taatagaggt atagagaaga gaggtgttca ttttggatac tattactcca acaaacttca
      301 aaaaaaatat aggtaaagtg ttgcaagaag tagttcattc aaattctgaa atagaaatta
      361 ctagggattc taaaaatgga ttgaacgatg gcgtcattat gatttctaag agaaagtggg
      421 agctgctaca agaag
//
LOCUS       DACCCS010000253          432 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.268, whole genome shotgun sequence.
ACCESSION   DACCCS010000253 DACCCS010000000
VERSION     DACCCS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.268"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..417
                     /locus_tag="IVK82_003039"
     CDS             <1..417
                     /locus_tag="IVK82_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6394016.1"
                     /translation="TSDDKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVDG
                     WHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPN
                     KKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          156 a           75 c           86 g          115 t
ORIGIN      
        1 acttcagatg ataaaccagt cattgatcca actagagcag gtgcagcaaa tcctacgctg
       61 acagatggaa caaatcacgc ccacattgat cagtttggag aaatcgaaaa cgcaaacttg
      121 catgtggatg gatggcacat tgctaattat aaatacgagt atattttcat tatggattac
      181 aatactggaa aagaattagc tcgagtaaga gctgatggaa tttatagacc agatgtaaat
      241 caagcttata atacttctgg aaacgttggt tatcatgtat ctttcaatat gcgtaatttt
      301 cctaataaga aagtctatgt aatgatgcgt gcaacgaatg atccagaagg gaacactaaa
      361 ggcggagcac aagattttca tgataaacgc tggtatttaa atattccgca acgataaaaa
      421 aagcccctcg at
//
LOCUS       DACCCS010000254          430 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.269, whole genome shotgun sequence.
ACCESSION   DACCCS010000254 DACCCS010000000
VERSION     DACCCS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.269"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>430
                     /locus_tag="IVK82_003040"
     CDS             <1..>430
                     /locus_tag="IVK82_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP6394017.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DACCCS010000255          428 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.270, whole genome shotgun sequence.
ACCESSION   DACCCS010000255 DACCCS010000000
VERSION     DACCCS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.270"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..129)
                     /locus_tag="IVK82_003041"
     CDS             complement(<1..129)
                     /locus_tag="IVK82_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP6394018.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSR"
BASE COUNT          156 a           71 c           51 g          150 t
ORIGIN      
        1 tcgactcata acaacaagtt ctgttgttaa tggatctaaa tttctttcta tacattcgac
       61 acttgcgtaa aagcttttca tatcgattaa aaagtaatca tttactggct cttttgaata
      121 atccatcata aataacactc cattctttca cattatacaa acatatgttc gatattgtaa
      181 agaagaaata gctaaataca taaaaaaaga agcataggta tgctatcacc ctgcttcttc
      241 ttttgatttg tatgatcaat ttattctatc actttactaa ttaatttgaa aatctaataa
      301 aaactctcac tctaaaagag tgagagactg caaattagag tacggacttt tttggagttc
      361 cgttctttaa tattaacaaa tgaataaaat ccttacaaca tttgagaata agttatcagt
      421 agacactt
//
LOCUS       DACCCS010000256          427 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.271, whole genome shotgun sequence.
ACCESSION   DACCCS010000256 DACCCS010000000
VERSION     DACCCS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.271"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>427
                     /locus_tag="IVK82_003042"
     CDS             <1..>427
                     /locus_tag="IVK82_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP6394019.1"
                     /translation="SLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGI
                     LDAYKPLMETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGG
                     GGIAYTSAKHAIVGFTKQLALDYAGQGIRVKGIAPGAIQT"
BASE COUNT          127 a           88 c          103 g          109 t
ORIGIN      
        1 tctctttgcg atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag
       61 aactccaaca agcggtatcg aaatgccagg atcgttttgg tgaagttacg attttactga
      121 atacatcggg gatattggat gcctacaaac cattgatgga aactgacgaa tcgctgtggg
      181 atcttattta tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg
      241 atatgatccg ccgaaaaagc ggaacaatca tcaacatggc ttcgattgcg ggtttggttg
      301 ctggcggagg tgggatcgct tatacaagtg cgaaacatgc aatcgtcgga tttaccaaac
      361 aactcgctct ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca
      421 tccaaac
//
LOCUS       DACCCS010000257          407 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.272, whole genome shotgun sequence.
ACCESSION   DACCCS010000257 DACCCS010000000
VERSION     DACCCS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.272"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..384
                     /locus_tag="IVK82_003043"
     CDS             <1..384
                     /locus_tag="IVK82_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6394020.1"
                     /translation="RKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIE
                     NQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTY
                     NQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          157 a           73 c           64 g          113 t
ORIGIN      
        1 cgaaaagaaa tctgtgatga ctttaaggct gtttatcaag ctaactcaaa agaagaagcg
       61 aataccttct tatccggcat gattgagaaa tggaagaaaa actatcctaa agtgacgcag
      121 tcactcatag aaaaccaaga cttattaact ttttatgatt ttccacctag cattcgtaga
      181 accatttact caaccaatct aatcgagtct ttcaataagc aaattaaaag atacagccgt
      241 agaaaagagc agtttcaaaa tgaagaatca ctagaacgct ttctagtcag catttttgat
      301 acatacaatc aaaaatttct aaacagaagc cataaaggtt ttcaacaggt aaccgataca
      361 ttagtttcaa tgtttactga gtaactaatt attttgcagg aggacaa
//
LOCUS       DACCCS010000258          401 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.273, whole genome shotgun sequence.
ACCESSION   DACCCS010000258 DACCCS010000000
VERSION     DACCCS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.273"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            10..>401
                     /locus_tag="IVK82_003044"
     CDS             10..>401
                     /locus_tag="IVK82_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP6394021.1"
                     /translation="MTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIATVGVGDN
                     PLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMGILS
                     IRDLANWNPYTIKNRLGVIGLQLYFHANG"
BASE COUNT          138 a           63 c           95 g          105 t
ORIGIN      
        1 attttaaaag tgacaaaatc actgaatctt tttacgactg aagaaacacg aagtcagcga
       61 aggaagaagc tcgctcaaat gatccaagaa cgtattaaag aagagttagg tttgattgct
      121 acggtaggcg tcggcgataa tccccttttg gctaaattag ccttagacaa tgaagcaaag
      181 cataatgaag gatttattgc ggaatggacg tacgaaaacg ttcctgagaa agtatggaat
      241 attcccgaaa tgacggactt ttggggaatt ggttcgcgaa tgaaaaaaag gctcaaccaa
      301 atgggaattt tgagcattag agaccttgct aactggaatc cttatacgat taagaataga
      361 ttaggtgtga ttggattaca gctgtacttc catgctaatg g
//
LOCUS       DACCCS010000259          395 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.274, whole genome shotgun sequence.
ACCESSION   DACCCS010000259 DACCCS010000000
VERSION     DACCCS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.274"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            74..395
                     /locus_tag="IVK82_003045"
                     /pseudo
     CDS             74..395
                     /locus_tag="IVK82_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          160 a           75 c           75 g           85 t
ORIGIN      
        1 gaatcggcta cactagacta gacagaaaaa ataaggtgtg tagaataata aaaaacacac
       61 tggaggattt ttcatgtcaa gacgtcaacg aagaacctat tcaaaagaat tcaaacaaca
      121 aatcgtcgat ctctatctcg ctggtaagcc tcgcgcagaa attattcgag agtatgagct
      181 tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct cattcaaaga
      241 aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga aaaataagca
      301 actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac gaaaagacaa
      361 gtaattgatg ccaacaagca taaatattcc atatc
//
LOCUS       DACCCS010000260          389 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.277, whole genome shotgun sequence.
ACCESSION   DACCCS010000260 DACCCS010000000
VERSION     DACCCS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.277"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            10..>389
                     /locus_tag="IVK82_003046"
     CDS             10..>389
                     /locus_tag="IVK82_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP6394022.1"
                     /translation="MTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIATVGVGDN
                     PLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMGIMS
                     IRDLANWNPYTIKNRLGVIGLQLY"
BASE COUNT          134 a           62 c           94 g           99 t
ORIGIN      
        1 atcttaaaag tgacccgatc actgaatctt tttacgactg aaggaacacg aagtcaacgg
       61 agaaagaagc tggctcaaat gatccaggag aatattaaag aagagctagg attgattgct
      121 acagtaggtg tcggagataa tccccttttg gctaaactag ccttagacaa cgaagcaaag
      181 cataatgaag gatttgttgc ggaatggacg tacgaaaacg ttcctgaaaa agtatggaat
      241 attcctgaaa tgacggactt ttggggaatt ggctctcgaa tgaaaaaaag actcaatcag
      301 atggggatta tgagcattag agatcttgct aactggaatc cttatacgat taagaataga
      361 ctaggtgtga ttggcttaca gctgtactt
//
LOCUS       DACCCS010000261          371 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.278, whole genome shotgun sequence.
ACCESSION   DACCCS010000261 DACCCS010000000
VERSION     DACCCS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.278"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>371
                     /locus_tag="IVK82_003047"
     CDS             <1..>371
                     /locus_tag="IVK82_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP6394023.1"
                     /translation="RRNTDQLKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNG
                     DFPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLL
                     NQIAKVGYNSTKKSYFYGLKI"
BASE COUNT          126 a           66 c           64 g          115 t
ORIGIN      
        1 cgaagaaata ctgatcaact gaagcaacgc gatacagtga ttattgcttg tgttatatgg
       61 ggcataatta atggctatac tagccaaaga gccacgtata gagcggtttg ttccgtctta
      121 tttcctaatg gtgactttcc tagtaggagt cgattcactc gcttaagttc aaacttagct
      181 tacactatca agattattcg atactttttc atcaagaagc tcaccaaagg tgaactagtc
      241 ggaattatag atagttttcc tagtccatta tgtaaaccag ttagaaatag acaagcaaaa
      301 ctattgaatc aaatagccaa agttggctat aactcaacaa aaaaatctta tttttatggt
      361 cttaaaattc a
//
LOCUS       DACCCS010000262          365 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.279, whole genome shotgun sequence.
ACCESSION   DACCCS010000262 DACCCS010000000
VERSION     DACCCS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.279"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>365
                     /locus_tag="IVK82_003048"
     CDS             <1..>365
                     /locus_tag="IVK82_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6394024.1"
                     /translation="FLDYEKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGE
                     FKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLT
                     EEVNAFKSRALNDKYVAIF"
BASE COUNT          134 a           63 c           59 g          109 t
ORIGIN      
        1 tttttagatt acgaaaaata tgatcgcact ggttttaatt caggtaattc gagaaacggt
       61 tcttactttc gatcaatcaa aaccgaatat ggtgaattaa cattggaaat acctagagat
      121 cgtaatggtg agtttaaaca acaaacttta ccagcctaca aaagaacaaa cgatacattg
      181 gaaaccacta ttatccattt attcgaaaaa ggtgttacga tgtctgaaat tgctgatttg
      241 atcgaaaaaa tgtacggtca tcactatact ccacaaacca tgtccaacat gactaaagtt
      301 ctgactgaag aagtaaatgc ctttaaatcc agagccttaa atgataagta tgtcgctatt
      361 tttat
//
LOCUS       DACCCS010000263          362 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.280, whole genome shotgun sequence.
ACCESSION   DACCCS010000263 DACCCS010000000
VERSION     DACCCS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.280"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>362
                     /locus_tag="IVK82_003049"
     CDS             <1..>362
                     /locus_tag="IVK82_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP6394025.1"
                     /translation="MQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSY
                     WQSIEDLIADMTDFINENKGDFTDWMNERKEEFDRWREEQENSFQDWREGQESDYLSW
                     FESIKDILKSIDPGGVMLA"
BASE COUNT          130 a           44 c           82 g          106 t
ORIGIN      
        1 atgcagtgtt tgcatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
       61 ggaacaactc aagacttctc ctattttgtc atcaatgctg tttcaaaaac agaaggagaa
      121 atgggttctt attggcaatc aattgaagat ttaattgctg atatgacaga ttttattaat
      181 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgatcgctgg
      241 agagaagaac aggagaatag tttccaagat tggagagaag gacaagaatc agattatttg
      301 tcatggtttg aatcaatcaa ggatattttg aagtctatcg atccaggtgg agtaatgtta
      361 gc
//
LOCUS       DACCCS010000264          362 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.281, whole genome shotgun sequence.
ACCESSION   DACCCS010000264 DACCCS010000000
VERSION     DACCCS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.281"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT          124 a           39 c           58 g          141 t
ORIGIN      
        1 taggactcaa ttgttcatag atgagcattt gtaattaagt tatattataa taaaaaaata
       61 cgaattttag tgacgccatt atattgtttt tatttttcaa ttgtaatatt tcatattact
      121 tccataataa ggagtatttt ctattggaat atcttagctc ttttaaggat ttctcctttt
      181 ttgtttctca ttttttaaga ttatttggat ttcctttaca acaaacttcc acaagtatta
      241 ttgacataga tgtaattgag ggaagggggt tccagtgcta gaataaataa tgaaaagagg
      301 taacaaatgc tagcgttaag aggtgaacaa gtttgattga aagacatgta aatttattaa
      361 aa
//
LOCUS       DACCCS010000265          352 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.285, whole genome shotgun sequence.
ACCESSION   DACCCS010000265 DACCCS010000000
VERSION     DACCCS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.285"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT          158 a           33 c           58 g          103 t
ORIGIN      
        1 atttataaat atcattcact catgataccc agctccgtat cacaaataat gatatttttt
       61 aagagaaaaa attatcccta ttttaaataa tagcaatagg tagtggtaga gagaaattta
      121 taaataactt tgttatttgc tttggtagcc aagtaagttg caaaagaaaa atagatactg
      181 ttaaaaagat aatgtagaca tagaaacaaa tacgtaaaat tttagttaga atgatggcaa
      241 ggagaaaaaa catgatagat gaaaaaaagt gtttaaaaaa tctattggaa gatatggatg
      301 atattgaaga aaaactatct aaatggactg ggaaaacaaa tatatttcag aa
//
LOCUS       DACCCS010000266          348 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.288, whole genome shotgun sequence.
ACCESSION   DACCCS010000266 DACCCS010000000
VERSION     DACCCS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.288"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(8..>348)
                     /locus_tag="IVK82_003050"
     CDS             complement(8..>348)
                     /locus_tag="IVK82_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6394026.1"
                     /translation="VDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTL
                     PQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARI
                     YLQQGLIFEN"
BASE COUNT          105 a           63 c           69 g          111 t
ORIGIN      
        1 tattttttta gttttcaaag atcagacctt gttgtaaata gatccgtgcc cgaaagttat
       61 aaaagttgcg ataaccaaac gcaattcgtt taatcagctt gatcttgtta ttcagcccct
      121 cagtgacgcc gtttgagtgg gacaccttaa aggcatttga aatacccgat tggtagcgat
      181 ggaagatcgt gaatttcttt ttaaacgact gaggtaacgt agatgaaatt gttttaaggg
      241 tttcttgaaa gccttccgta cctcgtttaa cgaaataata gcgtaataat tgacaaaaat
      301 gatacgcagt tcgcaattct tcgttgtagc ttaacagctc atcaacga
//
LOCUS       DACCCS010000267          344 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.289, whole genome shotgun sequence.
ACCESSION   DACCCS010000267 DACCCS010000000
VERSION     DACCCS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.289"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT          110 a           78 c           72 g           84 t
ORIGIN      
        1 aaagaagagc tgcagtccta aatgaggatt gtagctcttt ttgttttaca tagaaacagt
       61 aaaaatgaaa aggcctgttt aggaaggcac aagcgccttc tcaattgaaa gacctttctc
      121 tcactccaac cgccaccgcc gttcgagaag aaaggaagtg aacgctacgc gttcactaag
      181 ataaagggtc ttgcatagca agacaaaata gaaaaaattt cctctcacca aaaggttcgc
      241 tcctgttcgt tatctcttta ttgatttgtc agaccggcaa caagttgcct tttatcacca
      301 aaaagcgaca aggacaaggg taaatggcac gctgcgcgcc cttg
//
LOCUS       DACCCS010000268          337 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.290, whole genome shotgun sequence.
ACCESSION   DACCCS010000268 DACCCS010000000
VERSION     DACCCS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.290"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            122..328
                     /locus_tag="IVK82_003051"
     CDS             122..328
                     /locus_tag="IVK82_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP6394027.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          120 a           43 c           82 g           92 t
ORIGIN      
        1 gataaaattg aacaactgga cgggcaaatt gatgccttgc tttcagaaag agaaatattg
       61 atagaagaaa acaagcgatt gaaaaagaaa gtagcccaac caatgaaggg aggtggatag
      121 tatgaacaat agaacctttg atattttgaa atggatcgct atagtagtta tccccgcttt
      181 agcgactttt gtcggtcttg tcggaaaagg tgttaactgg cggtacacag atataacggt
      241 gatgattatt acaggttttg gagcatttct agggagtatt ttaggtgtat caaatcgaac
      301 gtataagaaa atttcatcag ataaatagga ggataga
//
LOCUS       DACCCS010000269          320 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.292, whole genome shotgun sequence.
ACCESSION   DACCCS010000269 DACCCS010000000
VERSION     DACCCS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.292"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>320
                     /locus_tag="IVK82_003052"
     CDS             <1..>320
                     /locus_tag="IVK82_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAP6394028.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGEREK
                     QFAY"
BASE COUNT          125 a           50 c           69 g           76 t
ORIGIN      
        1 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
       61 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
      121 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
      181 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
      241 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
      301 agaaaaacaa tttgcttatt
//
LOCUS       DACCCS010000270          318 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.293, whole genome shotgun sequence.
ACCESSION   DACCCS010000270 DACCCS010000000
VERSION     DACCCS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.293"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            32..>318
                     /locus_tag="IVK82_003053"
     CDS             32..>318
                     /locus_tag="IVK82_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP6394029.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCR"
BASE COUNT          111 a           52 c           70 g           85 t
ORIGIN      
        1 aaaataaaca actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg
       61 attaatagag aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa
      121 gacgttgatc gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc
      181 tactgtggta gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat
      241 tgtccgcttt gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta
      301 tacctgtaaa aactgtcg
//
LOCUS       DACCCS010000271          309 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.295, whole genome shotgun sequence.
ACCESSION   DACCCS010000271 DACCCS010000000
VERSION     DACCCS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.295"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            51..>309
                     /locus_tag="IVK82_003054"
     CDS             51..>309
                     /locus_tag="IVK82_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP6394030.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKW"
BASE COUNT          120 a           41 c           61 g           87 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggc
//
LOCUS       DACCCS010000272          304 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.296, whole genome shotgun sequence.
ACCESSION   DACCCS010000272 DACCCS010000000
VERSION     DACCCS010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.296"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            83..271
                     /locus_tag="IVK82_003055"
     CDS             83..271
                     /locus_tag="IVK82_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6394031.1"
                     /translation="MLKKTMLVLGSISLGVLGFSPMLLSVIGKNNSKGELKLNPNYYN
                     SPNEPMFISEKQNDGQDK"
BASE COUNT          113 a           53 c           48 g           90 t
ORIGIN      
        1 aatttaaaca tttgacaaac tatagaacag cacttaaaat gtgtatacaa gtgacagcgc
       61 tttaaaaaaa gaggtgcagc ctatgttaaa aaaaactatg ttggtactag gttctatttc
      121 tctaggagta ttaggtttta gccccatgct attatccgta attggaaaaa ataattccaa
      181 aggggaactt aaattaaatc caaattacta caatagtcct aatgaaccta tgtttatttc
      241 agaaaaacaa aacgacggtc aggacaaata atcctgccgt cgttttgctc taacttctac
      301 cagt
//
LOCUS       DACCCS010000273          275 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.299, whole genome shotgun sequence.
ACCESSION   DACCCS010000273 DACCCS010000000
VERSION     DACCCS010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.299"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            135..>275
                     /locus_tag="IVK82_003056"
     CDS             135..>275
                     /locus_tag="IVK82_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HAP6394032.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAK"
BASE COUNT           91 a           56 c           46 g           82 t
ORIGIN      
        1 attaaaacca tgtgaaactg cctcacggca gcttacaatt actttttcat cttcacccgt
       61 taagtgatga aaaaagaacc cctttctgct atggttaaag tgactaaacc aaccaaaaga
      121 aaggagttct tttcatgact agcttacaca aaaaccaagt aaaattcaat tcaaatatca
      181 ctatttctca tacaggtggt caattatcga gtgattcggg tctcgtccta gtgaaagagc
      241 tgatgaacat ctttggtttt tctgaactgg ctaag
//
LOCUS       DACCCS010000274          268 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.302, whole genome shotgun sequence.
ACCESSION   DACCCS010000274 DACCCS010000000
VERSION     DACCCS010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.302"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            87..>268
                     /locus_tag="IVK82_003057"
     CDS             87..>268
                     /locus_tag="IVK82_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6394033.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT           84 a           55 c           43 g           86 t
ORIGIN      
        1 tttacctatg aaatattttc acgtgttaaa gtattcctcg ttgaacacaa caaataaaac
       61 tttttaacga ataaaggaga tttttcatga catcacttaa aactttactt tttggaacaa
      121 ctttggctgc cggcgctgca ttcttcatgg gaacgactgc tcatgcggac gaagcttata
      181 ccgttcaatc aggtgatact ctatcaacga tttctcaaaa atacgttggt gataactcct
      241 tgatcaatgc cattgcagaa tcaaattc
//
LOCUS       DACCCS010000275          267 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.303, whole genome shotgun sequence.
ACCESSION   DACCCS010000275 DACCCS010000000
VERSION     DACCCS010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.303"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(35..>267)
                     /locus_tag="IVK82_003058"
     CDS             complement(35..>267)
                     /locus_tag="IVK82_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6394034.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          103 t
ORIGIN      
        1 tagaaggact gaccttctgt ctcagatttt tttgttattt cacttgaatc aattgattga
       61 tcaaaaaaat cctgattttc atgttctcaa atgatttaaa tccataagat aatcgtttca
      121 gtgtcttgat atggggaatc ttggcctcta ttttgccatt agaatacggg taaatcatag
      181 cattgtaaat accttcttca tacgttaaaa ggttttgaag tttcgtgcga agttgtgcat
      241 caagtgtttc aggaagattt tttaata
//
LOCUS       DACCCS010000276          257 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.305, whole genome shotgun sequence.
ACCESSION   DACCCS010000276 DACCCS010000000
VERSION     DACCCS010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.305"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(69..257)
                     /locus_tag="IVK82_003059"
                     /pseudo
     CDS             complement(69..257)
                     /locus_tag="IVK82_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           63 a           60 c           39 g           95 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttatt
//
LOCUS       DACCCS010000277          255 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.306, whole genome shotgun sequence.
ACCESSION   DACCCS010000277 DACCCS010000000
VERSION     DACCCS010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.306"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>255
                     /locus_tag="IVK82_003060"
     CDS             <1..>255
                     /locus_tag="IVK82_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP6394035.1"
                     /translation="PGFSHQMKIPITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSK
                     LVYTDSLQLDLFSDPQKQIDEENLDKIIDKIRQKYGFTSIV"
BASE COUNT           90 a           38 c           50 g           77 t
ORIGIN      
        1 ccagggtttt cacaccagat gaagatccct atcacagata atacaaagga attgcagaat
       61 tattgtcttt tcttgtttga taagtattat gaaggacaag aagtaaggca tgttggaata
      121 acctatagca agcttgtgta caccgattcg cttcagctgg atttatttag tgatccacaa
      181 aagcaaattg acgaagaaaa tttagataaa attattgaca agattcggca aaaatatggt
      241 tttacgtcga ttgta
//
LOCUS       DACCCS010000278          254 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.308, whole genome shotgun sequence.
ACCESSION   DACCCS010000278 DACCCS010000000
VERSION     DACCCS010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.308"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            46..>254
                     /locus_tag="IVK82_003061"
     CDS             46..>254
                     /locus_tag="IVK82_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6394036.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNA"
BASE COUNT           96 a           36 c           62 g           60 t
ORIGIN      
        1 gcacacttgt tggtggtcat gcgggcggaa atgggggaat taaaaatgat tgattacaat
       61 gatcgtggac gtttaaagtg ggcagggttt tatttatctg atcatactga agaaattgat
      121 gccgacaatg aacaacgaac cagtcaaaat ttagcaaaag aacaaatgac gactgaagag
      181 attgcggaag tattgaacgc tgcagtcata aaaaatagat cagtatctat tcaaaaagaa
      241 gaacgaaacg ctga
//
LOCUS       DACCCS010000279          242 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.311, whole genome shotgun sequence.
ACCESSION   DACCCS010000279 DACCCS010000000
VERSION     DACCCS010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.311"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>242
                     /locus_tag="IVK82_003062"
     CDS             <1..>242
                     /locus_tag="IVK82_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP6394037.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFKLPDIGEG"
BASE COUNT           75 a           50 c           65 g           52 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa
      241 gg
//
LOCUS       DACCCS010000280          241 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.312, whole genome shotgun sequence.
ACCESSION   DACCCS010000280 DACCCS010000000
VERSION     DACCCS010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.312"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..220)
                     /locus_tag="IVK82_003063"
     CDS             complement(<1..220)
                     /locus_tag="IVK82_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6394038.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFP"
BASE COUNT           63 a           45 c           46 g           87 t
ORIGIN      
        1 caggaaaaat gatttcggtt ctcccacaac gtcgcttatt ttctttgtat tgttcaaagt
       61 attcaagtgc tgttccacca catttgagaa agttatagac attgtaaatt gtttggcgtc
      121 cacgtttaag ctgcttcgca acgatagcaa ccggagtttc ttgatgaaaa tatgcttcta
      181 tcattacaag ctcgtttggt gtaagatggg tataagtcat ttatgttcac tctccttgta
      241 t
//
LOCUS       DACCCS010000281          235 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.313, whole genome shotgun sequence.
ACCESSION   DACCCS010000281 DACCCS010000000
VERSION     DACCCS010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.313"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..>235)
                     /locus_tag="IVK82_003064"
     CDS             complement(<1..>235)
                     /locus_tag="IVK82_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6394039.1"
                     /translation="YISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVE
                     TVFSSLEKLGCQNFNSRSVKGLESRFESILLAYS"
BASE COUNT           66 a           41 c           42 g           86 t
ORIGIN      
        1 cactgtaagc cagtaatatg ctttcaaatc tactctctag ccctttaaca gaacgtgagt
       61 tgaaattttg acaacctaat ttttctaagg aagaaaaaac agtctcaact gtttttcttt
      121 gttttccaag taagctgtgg tctagttttt ctgatttatc catgttttta cgtggcggaa
      181 cggatatagt aatgccctta agggcaagct tttcatggat tttatggcta atata
//
LOCUS       DACCCS010000282          234 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.314, whole genome shotgun sequence.
ACCESSION   DACCCS010000282 DACCCS010000000
VERSION     DACCCS010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.314"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT           68 a           42 c           41 g           83 t
ORIGIN      
        1 attctacgtt gtcgggttat ccagcttgca accgttcctt gtgggtaggc atataactca
       61 caaaattgtt ctacagtgat tcctaaattc ttgataatgt aaacattgat cgggtgcgag
      121 taaacaaatg tattgataga catggtgcgt aagcctactt tcttttatta ttgcgtttgc
      181 aataatagtt taacacacgc attattctag tatctactcc tatttgtaaa aaca
//
LOCUS       DACCCS010000283          233 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.denovo.315, whole genome shotgun sequence.
ACCESSION   DACCCS010000283 DACCCS010000000
VERSION     DACCCS010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.denovo.315"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DACCCS010000284        23728 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.guided.040, whole genome shotgun sequence.
ACCESSION   DACCCS010000284 DACCCS010000000
VERSION     DACCCS010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.guided.040"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            289..564
                     /locus_tag="IVK82_003065"
     CDS             289..564
                     /locus_tag="IVK82_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6394040.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            577..2655
                     /locus_tag="IVK82_003066"
     CDS             577..2655
                     /locus_tag="IVK82_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP6394041.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2815..4701
                     /locus_tag="IVK82_003067"
     CDS             2815..4701
                     /locus_tag="IVK82_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6394042.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4713..5660
                     /locus_tag="IVK82_003068"
     CDS             4713..5660
                     /locus_tag="IVK82_003068"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP6394043.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5680..6207
                     /locus_tag="IVK82_003069"
     CDS             5680..6207
                     /locus_tag="IVK82_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6394044.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6270..6923)
                     /locus_tag="IVK82_003070"
     CDS             complement(6270..6923)
                     /locus_tag="IVK82_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP6394045.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7055..7897
                     /locus_tag="IVK82_003071"
     CDS             7055..7897
                     /locus_tag="IVK82_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6394046.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8055..8951
                     /locus_tag="IVK82_003072"
     CDS             8055..8951
                     /locus_tag="IVK82_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6394047.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8954..9667
                     /locus_tag="IVK82_003073"
     CDS             8954..9667
                     /locus_tag="IVK82_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP6394048.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9687..10205
                     /gene="msrA"
                     /locus_tag="IVK82_003074"
     CDS             9687..10205
                     /gene="msrA"
                     /locus_tag="IVK82_003074"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6394049.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10202..10429
                     /locus_tag="IVK82_003075"
     CDS             10202..10429
                     /locus_tag="IVK82_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP6394050.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10581..11132
                     /gene="lepB"
                     /locus_tag="IVK82_003076"
     CDS             10581..11132
                     /gene="lepB"
                     /locus_tag="IVK82_003076"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6394051.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11215..11757)
                     /locus_tag="IVK82_003077"
     CDS             complement(11215..11757)
                     /locus_tag="IVK82_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP6394052.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11994..12392)
                     /locus_tag="IVK82_003078"
     CDS             complement(11994..12392)
                     /locus_tag="IVK82_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP6394053.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12652..13512
                     /gene="ylqF"
                     /locus_tag="IVK82_003079"
     CDS             12652..13512
                     /gene="ylqF"
                     /locus_tag="IVK82_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP6394054.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13505..14272
                     /locus_tag="IVK82_003080"
     CDS             13505..14272
                     /locus_tag="IVK82_003080"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP6394055.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14328..15185
                     /gene="dprA"
                     /locus_tag="IVK82_003081"
     CDS             14328..15185
                     /gene="dprA"
                     /locus_tag="IVK82_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP6394056.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15307..17385
                     /gene="topA"
                     /locus_tag="IVK82_003082"
     CDS             15307..17385
                     /gene="topA"
                     /locus_tag="IVK82_003082"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP6394057.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17354..18730
                     /gene="trmFO"
                     /locus_tag="IVK82_003083"
     CDS             17354..18730
                     /gene="trmFO"
                     /locus_tag="IVK82_003083"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP6394058.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18928..19833
                     /gene="xerC"
                     /locus_tag="IVK82_003084"
     CDS             18928..19833
                     /gene="xerC"
                     /locus_tag="IVK82_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP6394059.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19870..20418
                     /gene="hslV"
                     /locus_tag="IVK82_003085"
     CDS             19870..20418
                     /gene="hslV"
                     /locus_tag="IVK82_003085"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP6394060.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20432..21832
                     /gene="hslU"
                     /locus_tag="IVK82_003086"
     CDS             20432..21832
                     /gene="hslU"
                     /locus_tag="IVK82_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP6394061.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21850..22641
                     /gene="codY"
                     /locus_tag="IVK82_003087"
     CDS             21850..22641
                     /gene="codY"
                     /locus_tag="IVK82_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP6394062.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22723..23598
                     /locus_tag="IVK82_003088"
     CDS             22723..23598
                     /locus_tag="IVK82_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP6394063.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8212 a         4091 c         4999 g         6426 t
ORIGIN      
        1 tgattcatca gtcccataaa ttatagagcc gatttttgtc gcagccccat ttttttgttg
       61 tgataaaaga acaagaaagg caggaatggg aaagtaaaca tccttttgtc agaaactttt
      121 atctttctta agttttactt gatatctgat actatttgga atagaatgag gcgttgaaaa
      181 atttatggaa atttctaact agcaataaac gatgaaacaa tgaaatattt ttcatccaaa
      241 aagaactttg ctataatgat ggaagtttgt gtataggaga tctggaaaat gagagagagt
      301 tttgaacaac aaaaaaaact gttgcatgat cgctatggcg ccctttcgat ggatgaccgc
      361 cgtcaaatac tttgtaagtt gcgtaaaaga aatatattga tgtatcgtca gttggaacgt
      421 ctgaaacatg atctgcttcg tttggagtca aaacgtgtac agtgtgaatt agaggggaac
      481 caaacacagg ttgaagtagt cgaaaccaaa attttaaaga aaaaagaaca atttttaaaa
      541 atgcttacac aaaataaaaa ataaagggga tggacgatgg aatttgctga gttagttatt
      601 gtatttgcag taacgatcac cttatcgaac atagctagcc gcattttgcc aatgattcca
      661 gctccactga tccaaatttt ccttggagtg gtattaggtc tgacagagtg gggacaatca
      721 atcgattttg aacctgaatt gttcttagtt atgattattg caccattgtt atttagagaa
      781 ggagaaaaag cagatatttc ttcgattctc aaaaatttcg ggacaatttt gttcttggca
      841 tttggaggag tcattttgac ccttgtcggg gtaggtgcga cgttatcatt cctgttgcca
      901 agtgtcccac ttgctgcctg ctttgctttt ggagcagcgt taggtccgac agatgcggta
      961 gcagtcagtt ctctttctgg acgagtgaat atccctaaaa aggcgatgca tattttggaa
     1021 ggagaaggtc ttttgaatga tgcatctggt gttactgcct tccagtttgc tcttggtgcg
     1081 ctgatcaccg gatctttttc agcagtaaac gcggggatga gtctagtagt ttctagtatt
     1141 gggggagctt tgatcggctt tttgcttgtc tggttcaaac aaaaaataat tcatttgatc
     1201 gaaaaagcat cagcacaaga tgtcacaggg tatctgctga tcgaactttt gcttcctttt
     1261 ctagcctatg tattggcaga ggcatttgat gtatctggaa tcattgctgc agtggcagca
     1321 ggtattctcc aagcttctgg ttttaggaaa atctctgtgt ttgatgcaga attgtcgagc
     1381 ttgtcccaca gtacatggac aactattgct tttacactga atgcattagt atttatattt
     1441 ttaggaatcg aattgacgca agtgttctcg ccagtatggg gagacggttt atatccaaac
     1501 ggtcttttat tagcgattat cgtattaatc tctgtcatgc tttttgtgat cagattcatt
     1561 tctatcagtc ttttttatgt attcaaagat ggcagtaaaa agttcaaaaa acagttgaac
     1621 gagatcttga tcttaacatt tggcggtgta aagggaacag tcagtttggc tacgattttt
     1681 attttgccat tttcaatcaa taatatgatg ttttaccagc ggtctttgct tttgtttctg
     1741 acggcgggtg tcatcttagt gactttggtc atagggatca ttgtcttgcc tatgctgacg
     1801 gaaacagagg aagcgaaaag cacggatttg aatgctttga tgattttaga agaggtcgtt
     1861 gaagttttgc gaaaagaaat aaaggaaatt gatcgtaata caaaagaatt tttagccact
     1921 gaagcagtga tagaaaatta tcaagaacgg atacgtgatc tgtacttgga agatttaacc
     1981 gatgatgaaa agcaagaagt acaagaaata caagcactga ttttgtcgat cgaacgggat
     2041 ggattggatg aaagttatcg ttcgggcaag ctttcttcta atggttatcg tttttactcc
     2101 cgttttcttt ctcgattcga acattctatc acaagccaga ttttgtcttt tatcggcttt
     2161 tggttcattg ttgtccgtag gttaatgcga atcgttctcc atccgaaaat gttttggcaa
     2221 agaagatatg ctgatcgtca gacatttatt agtgatgaag atatccaaga aatcagagag
     2281 acatatcaaa aaaatactca gttgattatt gaaagtttag ataacttgac tgatatttat
     2341 gatgaaacga tggttcaatt ttttatccag caacgcaaac ttgaaggaat aaaaatgaac
     2401 tcggggaatt tgatttcttc gtggatgatc cagcaagata gtctgtttac gaaaaaaatg
     2461 ctaagaggtt attatcttga aagaaaaatc attgatgaat acgaagttgc tgaaaaaatc
     2521 acgacgtttt ctgcgaacag ctatcgcagg aatatcaatt tgttagaatc ttatacgatg
     2581 aacaaaccat ctgataactt ttctttccga tttgcgtttc gagcaaagtc aaaaaaaacg
     2641 agtccaataa aataaaaagg taaaaatttt caagttttat aaaaaacaaa acatatcaga
     2701 acaggatcga atcggtcctg ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa
     2761 attttcctga agttcactat ttttatgata caatatccaa gataaggagg gaacatgttg
     2821 aaagaattaa aagtaactga cctcaaaaaa acttatggtg aaaaagattt gtttgaccag
     2881 atttcttttt tggtccacga aaaagaccgt atcgggttga ttgggacgaa cgggacgggt
     2941 aaaacaagtc tcttaaatat tattgccggt atcgatagca gcgacggtga tcggcaaaca
     3001 gtgttttatc caactgatta ccgtattggt tatctttctc aagcagctga attttccgat
     3061 gaattaactg ttctccaagc agtctttcaa ggaaacagcc ccttgatcca aactgttcga
     3121 gcatatgagg aagcattgat cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa
     3181 cgttatgcta aagcagaaga acaaatgaat aaagaagatg cttggactac agataccaac
     3241 gcaaagatta ttttgcaaaa attaggaatc agtgaattag ataaaaaaat cagtacttta
     3301 tcaggtggtc agaaaaaacg agttagtctg gcgcaagtgt tgattgatga acccgattta
     3361 ctattactag acgaaccaac aaaccattta gattatgaag cgatcgagtg gttggaaaac
     3421 tatttgaaac aataccgtgg cgcactattg atggttaccc acgatcgtta ttttctagat
     3481 agagtagcaa accgaatctt cgaactttca tttggaaaat tatacgagta taaaggaaat
     3541 tatgaagctt acgtcttaga aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa
     3601 aagcgcaagc ggctttataa acaggaatta gcttggatga gggctggcgt acaggcacgt
     3661 ggcaccaagc agcaagcacg gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa
     3721 gtaaaccaag aagatgattt agagctgaac ctagcgacac aacgtttagg gaaaaaagta
     3781 ctggaaatca aagatggcag ttaccgtata aatgaccaaa cactgctaga acatttagat
     3841 ttattgattc aatctcggga aagactaggt attactggta aaaatggtgc gggaaaatcg
     3901 acattgctta atattctagc tggcagaatt cccctggata gcggtacaat gtctatcggt
     3961 gaaacggttc atcttgcgta ttatacacaa gaaaatgaag aaatggcacc agataaacgg
     4021 atgattgctt acttacaaga agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc
     4081 ggagtagcag aattacttga acgattttta tttccaagat ttatgcatgg gacactgata
     4141 ggcaaacttt caggcggaga aaaacgccgg ttatttttgt tgaaactgct gattcagcaa
     4201 cctaatgttt tattattgga tgaaccgacc aatgatttgg acatagcaac attgacgatt
     4261 ttggaagatt attttagaag ttttccaggt gctgtgatca ctgtttccca tgaccgttat
     4321 ttcttggaca aggtagcaga taagttatta gtattccaag gaaacggaaa acaagaactt
     4381 tattacggaa acatgtccag ttatttactg aagcaaaaag agacccagca gcctgctgaa
     4441 aaagcgaaac caaaaataca gagcaaggaa cctgctgaaa agaagaaatt atcttacatg
     4501 gaacaaaaag aatgggaaac gattgaagat gagattgcag aacttgaaga aaaaatctca
     4561 ttgctccaag aagagatgaa tcatcaagga gataatttca ctcgattaca agaattgcaa
     4621 aacgacgtat ctgaaacaga agcacaactt gaagaaaaaa tggcacggtg ggaatacttg
     4681 agtgaatggg tagaagatta ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa
     4741 gaaaattgtt ggaagaaggg catcgtaaag aagacagaac aggcacaggc acattaagct
     4801 tattcggata ccaaatgcgg tttgatctgc aaaaagggtt tcctttgtta acaacgaaac
     4861 gagtaccttt tggattgatc aaaagcgaat tgctttggtt tttaaaagga gatacgaata
     4921 tccgctatct attacaaaat aaaaaccata tttgggatga atgggcattt gaacgctaca
     4981 tcaaaagtcc agactataat ggaccagata tgactgattt tggccataga tgtttgacag
     5041 atcctgaatt caatgaagaa tataaaaagc agagcaagct tttttgcgag aagatcctaa
     5101 ccgatgattc ctttgctgaa acatacggcg atcttggaca catctacggc tatcaatggc
     5161 gtcactggga aacaaaagac ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa
     5221 tcaaaaagac tcctgattct agaagattga tcgtttctgc atggaatccg gaagatgttc
     5281 ctagcatggc tttgcctcct tgtcatacga tgtttcaatt ttatgttcaa gagggacggc
     5341 taagctgcca attgtatcaa cgcagtgggg atgtgtttct aggcgttccg ttcaacattg
     5401 caagttatgc gttgctgact cacctaattg cacatgaaac aggtttagcg gttggcgaat
     5461 ttgttcacac attaggagat gctcatcttt atatcaatca tatcgaacaa atgaaagaac
     5521 aattgagccg agaaatacgc gcatttccga ctttagtttt gaatcaggaa aagcagtcag
     5581 tatttgattt tgagatggaa gatatccaat tagaaggata tgacccacat ccggcgatca
     5641 aagctcctat cgctgtttag aatcagaaag gatgagaaaa tgttagctgc aatttgggca
     5701 caggatgaaa acggattgat tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat
     5761 ctgaaattct tcaaacaaat gacagaagca aatacactag tgatgggacg aaaaacattc
     5821 gaagggatgg gtagccgtcc gttgccgaat cgtcagacga ttgttttgac gagagattct
     5881 tcctataaag cagaaggcgt gcatgtcatg catagtgtgg aagacgtgct tgctctggaa
     5941 aaagaaacag acggtatctt tttcatcgct ggagggtcag ctgtttatca agaatttctg
     6001 ccattttgta cgatattata ccgtacagtc atccatcatg tattcgatgg agatgcttat
     6061 tttccacctg ttgactggtc tgattggtca ctgatcaata ctagtcaagg agaaatggat
     6121 gaaaaaaatc gttatccgca tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa
     6181 agcgggacag gcgaattgac gatataaaaa agctatggat agactagctg ttatttagag
     6241 ctgtcccatc catagcttta ttttttttat taagtaaaga ataaaacaga gaagtacata
     6301 aaacctgcac ctaacataac aaacaaatgc caaacaacat gcatatagcg gatagaattc
     6361 aagctataaa agaatgcgcc tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca
     6421 ccagtcattc caagagagtg ataaagagga acagcagcca cgacacagag ccaccccata
     6481 aaaatataga tgatcgtaga tacttttgat actctctccc gtttatgcaa cgtcaacgct
     6541 ttatatataa tgccaagaat cgcaaacaac cagattaata caaataaagt ccaacctagc
     6601 catcctcgga tactcagcaa acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc
     6661 gaactatgat cgaatacttg gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac
     6721 aaagtagacg ttaaaaataa aagaatcatc atcgatccat agatagcata agagacaata
     6781 tggagggagg aatgcaagtg ggcacctttt actaaaagaa ccactagtcc cgcgatactc
     6841 aatccagtcc caattccatg ggtgactgca ttaaacactt catttaatat ttcatacgtt
     6901 cgattaaatt tcgttttttc catgaaaacg gctcctttac taggtgtaat taatgaaaaa
     6961 tcattatttg tctgttatac taaaaataga aattgtctta catgtagttt aacatgaata
     7021 aggcatcttg ttaaagaaag agagagagaa aataatgaca aatgtaaaaa tcgtaacaga
     7081 ttcttcatgt acgatggaaa aaaatgtacg cgatgcttta ggcattcgtg tcatgccgct
     7141 atccatcatg atcgacggaa cagtatatcc cgatgatgac caactagacg gcgaaagatt
     7201 tatggaaatg atggcgaaag caaaaaatct gcccaaaaca agccaaccac caattggaga
     7261 attcatctcg atgtatgatg agctaggaga agacggcagt gaagtgattt cgatccatat
     7321 gacaaaaggc ttgagtggaa cagtagaagc agcaagacaa gccagtaatc taacaaaaac
     7381 gaaagtaacg gttatcgaca gtgataccac tgaccaagga ttatcttttc aggtaattcg
     7441 agcagctgaa ttagcaaaag aaggtaaaac agtagagaaa atccttgaag aagtcaaaaa
     7501 agtacaagac aatacaaaat tatacatcgg aatatccaca ctagataact tggtaaaagg
     7561 tggaagaatc agccgaacaa ctggtttgct gtctagtgtt ttgaacatac gcgtggtgat
     7621 gaatttcgcc cattctgaat taatccctgt aactaagggg cgaggaaaga aaacgttcga
     7681 taaatggttt aataatttga aaaatgaact gaaagaattg ccaaatgtgc gacaaatcgg
     7741 tatttcacat gcggatgcaa aagaattggc gcaagaattc aaagagggtt tgcaagaatt
     7801 attcccggac atgcatatcc ctgtactgca tacgaatccg gttatcgcta cgcatactgg
     7861 taaagacgcg tttgcaatca tgtattatgt agattagtcg aaaagaggat aagccctcag
     7921 cctgaaaggc ttgcagctta ttctcttttt ctttataaaa ttaggaaaat aattgttttc
     7981 ttttttcata aagactacct ttttgataaa atttttgata acattagaac caatcgaata
     8041 caagaggtga aaaaatgaaa ttaacgaaac gacatttgat cctcgttttg ttcccattat
     8101 taattgcgat catcatctat gcaggtttag ctgcttctat accgaaagct ggcccactaa
     8161 tgaaaccagt aactactgtg agagaagatc caaataaaaa agaaaaactt cactacatag
     8221 ctatcggaga ttcgctgact gaaggagtag gagatcaaac caagcaagga ggattcgttc
     8281 cccttgtcgc aaatgatcta aaagaccgtt ataatttgac cgcggttgaa acagaaaact
     8341 atggtgtaaa tggtgaacga agtgaccaga tcttaaagcg agtaaaaaca aaagaagaaa
     8401 ttcgaaacaa tatcaaaaca gctgatttta tcacactgac tgttggcggt aatgatttga
     8461 tgaaagttat ccaaaataat ttattcgggt taacggtcaa ttcctttaaa aagccactga
     8521 aaaactatca ggaaaatgtc acagaactat tggaagaatt tcgatccctc aataaaaccg
     8581 cacctattta cattttaggc atctacaatc cattttattt gaactttcct gagatcactg
     8641 acatgcagac aattgttaac aattggaaca aaggaacaga aacggtgaca aaaaaaatgc
     8701 cgaactgcta ctttatccca atcaatgatt tgctttatca aggattgaat caggaagtag
     8761 gtgtcacaga agaaggaaca gatcaaacaa ctagcacgac agaaaatcta aatggcatca
     8821 aaaataatgt gctctatgaa gaagatcatt tccatccaaa taatttaggt tatcaattga
     8881 tggcaaatgc agtgagagat cagctgatcg agacacaaaa ccaatggtta gagaaggagg
     8941 atgatcaata aacatgaaaa caagatctga cgtatcttca aacaaaacaa agaaaacaaa
     9001 ttactggaaa ttcgccttcc ttatccttct tggggtagta cttggttcga ccgtattttt
     9061 aggcagccgt atttttgcta atcgggaacc agaactccca gaaataccag ctcttacgga
     9121 aagacaagga gatcctgtct taacgatcaa ttcaaataaa gaaaaagtca atcaaatcat
     9181 cagctttttc cttagtgagt atcaaaaaga tagtgatatt gaatataaat tttatttgga
     9241 aaatgaagca ctactcaatg gtactttcga agtactaggt tttcctatcg atttctattt
     9301 atattttgat ccgtatgtga tggaaaacgg aaatgtccaa ctaaaagcta gaagtctttc
     9361 tatcggaaca ttgagtttgc caatcaaaga tgtgatgaat atgatcaaga gaaactataa
     9421 attaccagat tggatcgaag tcaatacaga tgatctgact atcatgctta ggttggatca
     9481 attccgaatg caaaacggga tgtatattaa agcagataaa atcgacttag taaatgatga
     9541 cattcggttt agtctatatt tgccaaaaga tacctctgat gcttcaaaca actcaaatac
     9601 caacaaaact agcaccagca gtactgaaag caagacaaaa gaaaataaaa aatcatcgga
     9661 tgagtagtat tagaggggga atcataatgg aacgagcaat atttgcagga ggctgcttct
     9721 ggtgtatgat ccagcctttc gacacgcttc ctggtatcca tacagtgatg tcaggatata
     9781 ctggagggca tgtaccagat ccgacttatg aacaagtaaa aagccaaacg actggacata
     9841 cagaagcagt ggagattatc tatgatccaa cattgatttc atatgaacaa ttagtagaac
     9901 tttactggca gcaaactgat ccaactgacg ctttcggaca attcgaggat cgcggtgaca
     9961 attatcgccc ggtgatcttt tatttgacag aagaacagcg gatcattgct gaaaaaagta
    10021 agaaacagtt agctgaaagt ggacgtttcg aacagccaat cgttacaaaa atcgaaccag
    10081 cccaaccatt ttatctagca gaagaagacc atcaagagta ttataaaaag catcctgaaa
    10141 attttaaaag aaaccatgca cgccgggctg cctttatcga agaaaattgg aggaacgaag
    10201 ggtgaatcga agcttttttc attatttaat gacactgcga ggcggtggca cacatgatgc
    10261 tttatctgtt ttcgctacag aagccggtaa agacatccaa tttcctaagc acagctctag
    10321 ctacgaagaa atttctaatt atctggaact gaatgtcgac tatttgccgt ccatggatat
    10381 ctttgacgaa gcatgggaaa aatatttaga aaataatcat gacatataag agatagtttt
    10441 gtactttatg atcaaaaaag ttttttgtcc aatagcctaa ttgaaagaat tatttttatt
    10501 tcagctatct agtcgcattt ttttgatatt tttcttataa taaagaaaga atgaaaaaaa
    10561 gagggatgga ttgatagaga ttgacaaaaa aacaacgcta tattaatcgc ttctggcttg
    10621 tattcaaata cttacttgtt tctgtattcc ttgcgttcat gttgcgggga tttttattca
    10681 tcccggtacc agtggaaggc aattcaatgg aaaacgtgtt gaagcaaggt gatatggttg
    10741 tgatggaaaa attttcggag atcagacgat ttgatattgt tgtttttcaa ttagcagatg
    10801 gcacgattta tatcaaacga gtaatcggtc ttccaggaga aaatgtcagc taccaaaatg
    10861 atcaattgaa gatcaatggg aaagtagtta aggaacctta tctaactaaa aacctgaaat
    10921 cagatcatgc aaatgcatcc tatacgacgg actttacact acaagagcta acgggacaat
    10981 ccaagcttcc ggaagataat tattttgtac ttggagataa tcgaagagtt tcaaaagata
    11041 gtcgttcgtt tggcacgatc aataaaactg atattctagg taaagcaaga tttgtctatt
    11101 atccacttga tgaaataaaa tggatccagt gagaaacaat aaagcgtgca gtctgttgag
    11161 actgcacgct ttattgttta atccaaaatc agtttactac cttagtacgt aaaatcaaac
    11221 agctcgttta aacacttttc tagcctctaa agttttgata aatgaataaa caaccattat
    11281 ttgaataggg atttggatca tattcctgat gaatctacct agaaaaatta ctttgaaatc
    11341 tgcaccaaac aaaacagaca tcagccaaga attgataaaa aggctgactg ggatcataat
    11401 caataattgg gcagcgatga cggaaggcag gtttatcttt ttatgatata aaaataagcc
    11461 attaataaat ccaccaagga aagccgtgat aagaaaagca gcttgaaacg ttcctgaacc
    11521 aaatatcgtg aaatttaaag tgttcgacaa catttcaagc aaaccagccc agatcggtcc
    11581 atatagataa cccataagcg ccatacttaa aaatccgaat gtgatttgaa aataagcagg
    11641 accaatcgct atcttgtata agatgacatc aagggctaat aaaagagcag aggtcgacaa
    11701 caatcttgca ttcatttcgg gcaatctaaa aacgtctttt gcaatcaaag tgttcaaaat
    11761 attctctcct ttgtcagtta ataataaaag gataccataa aatcaccaat gtgtcatgac
    11821 acattggtga tttacataca ctggcatcta acaattaaaa aaagggtgag aagactgctt
    11881 ttataaactg taaaaaagag attgtacgaa taaaaaaaat agaggcggag aatacttatc
    11941 gtatcatccg cctttgtttt tttgttataa aaaacgtcag attagttatc tgcttacatc
    12001 ttcatccata cgggttctaa tgtatttccg tctagatctt ggacttctaa tgaaaacatt
    12061 tcttcttcag gcattcccat atctacttcg tggaaagtac caccattttc ttttgcaatt
    12121 tgagcaaatt tttttacttc atccgcattt tcaacactaa acgaaatcaa agcaccactt
    12181 gtttttttat tatcagctat ttctttatct tttataaatt ttttataaaa atcatgagag
    12241 agaagcatta tccaaaaagt gtcatcccat accattgaac cagcttcttc agtagaaaat
    12301 tcttcatttt ttttaaaacc cagcttttca taaaaagcaa cggaacgatt gacatcagca
    12361 acaggaaaat taacaaaaac catactattc atttgtttcg tctcctttct gttttgttcg
    12421 tctatcatct cttatttagg aaacgctgtc aaaaatttag attcatatat agaaaccaaa
    12481 ataaaaattt gcatcatcaa aaaagtgaag atgtgataga atgaggactg gttcactttc
    12541 acttaactaa tagatggaat ttctttataa taaaaaagtg aatttatatt gaaaaagaaa
    12601 aaaacaagta aagccgggga atattccggt atgaaaggaa tggagagaac aatgaccatc
    12661 caatggtttc cgggacatat ggcaaaagcc cgacgagaag tttctgaaaa aatgaaatat
    12721 gtggacatcg tatttgaatt ggtagacgca cgattacctt tatcttctcg taatccaatg
    12781 ctagatcaga tcattcagca aaaacctcgc cttgttttat tgaataaagc ggatctggca
    12841 gatccacaac agacaaagct gtggcaagaa tattttcgta aacaaggcca tcttgcatta
    12901 gctatcacgg cacaagaaag taaaggaatt aaaggtctag tacctgcggc taaagaagca
    12961 ttgaaagaaa aacgggcaag agatgctgct cgggggatca agcctcgcgc tattcgggca
    13021 atggtcttag gtattccaaa tgttgggaaa tctacgttga tgaatcgact agtaggcaag
    13081 aaaattgcac agacaggaaa taagcctggc gtaacaaaag gtcagcagtg gcttcgttta
    13141 ggtaatgagt tggaattgtt agacacacca gggattttat ggcccaaatt tgaagatgaa
    13201 gaaatcggca aaaaactagc gctgacaggc gcgatcaaag accagcttgt ccatttagat
    13261 gatttagcga tttacggatt agactttttt gcccgatatt attcaggaag aatcagcgat
    13321 cgttatggtt tatcaatcga gcaagaagaa aaattggcac cggaattact gatggatatt
    13381 acagcaaaac gtgggtataa agaagactat gatcgtggca gcgaattagt cgtgcatgat
    13441 attcgtcaag gaaaacttgg acgatacaca ctggatcgtt gcgaagaaat ggagcaaaaa
    13501 agtgatgact gaatccatcc aaagtatcaa agaacgattg aaaagcatac agagaataga
    13561 tgatcctaca ctagaatcat taagaaatga tccacgcaaa ggcgtgcagc aagcactggc
    13621 ttcttttgaa cgacgattga aaaaggaagc attaattaag gaaaaatatg aagaaatgtt
    13681 ttgctttgaa aagcaagggt acgcacaagg tcaccaatgg attgccggca ttgatgaagt
    13741 aggaagagga ccattagctg gacctgtcgt ggcagctgca gttatcttgc cgaaaaatga
    13801 gatcatatta gggctgaatg actccaaaca attatcagag aaaaagcgag aacttcttta
    13861 tcaacaagtt caagaaaaag ccctggcaat cgggatcggg atcgttgatc agcagacaat
    13921 cgatgatgtg aatatttatg aagcaagtaa attagcgatg atccaagcag tagaaaaatt
    13981 gtccattgta ccaagctatt tgttgatcga tgcgatgacc ttggatttgc ctattcagca
    14041 ggaaaaaata atcaaagggg atgcacgatc tgtgtcgatt gctgctgcaa gtatcattgc
    14101 taaagtatat cgagaccaat tgatgaaaga atatgacgtg ctttatcctg gatatggttt
    14161 ctcgcataat gcaggttatg gaacaaagga acacttagaa ggacttgaac gacaaggaat
    14221 tacgccaatc catcgtaaaa gttttgcacc tgtaaaaaaa tatttacaat aaaaaacagc
    14281 cgcttttacg tatcttatta gaaagagaga cgaaaggcgg ttttatgatg tatcaaatag
    14341 aagaaaattt attgaaactt gcttatacaa aaggaatcag tagtcaagga aaatgggcac
    14401 ttgctaactg gatgatgcag tatccttata aagaaatcgg atttgaagac atcattaaga
    14461 taggtaaaat aaccaaacac cgggagctgt ttcgcaaaag ttggcaggaa attcacgcta
    14521 actggggcaa aatccaatcg aaacagtcgt ttatcacttg ctttgatccc aaatatcccc
    14581 ctcagctgct tcacttgacc tatccgccaa ttgtactgtt ttataatggt gatctatctt
    14641 tactttcttg taacatgtta agcgttgttg gtggaagaca cacttctgct ttagccaaaa
    14701 aaacagttta ttatttacta aaaccagtga tagaagctgg atatgtgatc gtcagtggct
    14761 gcgcgaaagg gatagataca tacggtcacc aagcagcaat ctctcatggt ggacggacaa
    14821 tcgcagtcat tggaacaggg atagagcaag tctatcctgc tgaaaatcgt gacttgcaag
    14881 aggtgattgc aaatgatcat cttcttttga gtgaatatcc tcctcaatcc ggcccgaaaa
    14941 agcattgttt tccaatgaga aatcggatta tcgcagcttt gtctaaagga acatgtgtct
    15001 ttgaagccaa gaaaaaaagc ggctctctga taacagcaca gcaagcattg gatcttggaa
    15061 gaacagtttt ttctgttcca ggaaatattt taacagaaca ttctaccgga tgtcatcatt
    15121 tgatccaaga cggtgcagtt tgcacggttt cgggtgaaga tatcttggca gaatttaaag
    15181 aataacacca tacttttcat ttgcttttct tgacaaacgt tttctgaatg gatatgatta
    15241 gctacgattt gtatgaatcc tcttttaata gtaggaacac gacaatggaa aggagtccat
    15301 tcagtaatgg cgtataaata tttagtgatt gtagaatcac ctgcaaaagc caagacaatt
    15361 gagaaatatc ttggacgaaa ctataaagta atggccagtg ttggccatat aagagattta
    15421 ccgaaaagta aaatgggtat cgattttgaa aacaattatg aaccccatta tatttctata
    15481 cgcggaaaag gcgatgtcat caaaagcctg aaagccgcag caaaaaaagc tcaaaaagtt
    15541 tacttggcaa gtgacccgga tagagaagga gaagcgattg cttggcattt agcgtaccta
    15601 cttgggttgg atctgaaaga aaaaaatcgg gtggtcttca atgaaatcac aaaagacgca
    15661 gtcaaagcag cttttaagga accaagaacg atcgatgtag atttagtaga tgcacagcaa
    15721 gctcgtcgta ccttagacag aatcgttggt tattcgatca gtcctattct ctggcgtaag
    15781 gtcaagaaag ggttaagtgc aggacgtgtc caatctgtcg ctttaaaaat tattattgac
    15841 cgtgaaaaag agatccgaga atttgttcca gaagaatatt ggagcatcga cggtaatttt
    15901 aaaaaagctc gcaagaaatt caaagcaaat ttctggggaa tcgacggtaa gaaaaagaaa
    15961 ttaccagatg cacaaagtgt aaaagaagtc actgctagaa ttgatggaac agacttcgaa
    16021 gttaaaaaag tagaaaagaa agagcgcaaa agaaaccctg cgaatccttt cactacaagt
    16081 agcctgcaac aagaagctgc ccgtaagtta aactttcgaa caagaaaaac catgatggta
    16141 gcacagcaat tatatgaagg aatcactttg ggtaaacaag gaacagtcgg cttgattaca
    16201 tacatgcgta ctgattctac ccggattgcc gatacagcaa aagcagaggc cgcagcattt
    16261 attgaagata cgtatggaaa agaattttct agacatgatc accgaaagat caaaaatgct
    16321 caaggcgcac aagatgccca tgaagctgtg cgtccaacga gtgtgcttcg tacaccagat
    16381 gaactcaaac aatatttaga taaagatcaa ttgaaacttt atacattgat ctggtcacgt
    16441 tttgtggcta gtcagatgac accagctatt ttagatacaa tgaaagtgac attagaacaa
    16501 aatggtgtca tcttcattgc gaacggttca aaaatcaaat tcaaaggatt catgcaagtg
    16561 tatgtggaag gtagagatga cggcaaagaa gagaaagaaa atattcttcc agaacttgaa
    16621 gaaggcgatg tagtccaatc tgttgatatt gaaccaaaac aacattttac tcaaccacca
    16681 gcacgtttta gtgaggctac gttgattcgt gcactagaag aaaacggggt agggcgacct
    16741 tccacttatg caccaacact tgatacgatc caaagacgct actatgtaaa attgacacaa
    16801 aaaagatttg agccgactga actaggagaa attgtcaatt cattaatctg tgaattcttc
    16861 cctcaaatcg tcgatatcca tttcacagca gaaatggaag gagatttgga taaaattgaa
    16921 gaaggcacgg aagcgtgggt caaagtggtt gatcgtttct ataaaccgtt tgaaaaagaa
    16981 ctgacgaatg cggaagaaaa aatcgaaaaa attcaaatca aagatgaacc agcaggattc
    17041 gattgtgatg tctgtggaca tccgatggtg ataaaattag ggaaatatgg gaagttttat
    17101 gcttgcagta attttcctga ttgtcggaat acgaaaccaa tcgtaaaaga aatcggtgtg
    17161 acttgcccag tatgtcatga aggacaagtc attgaacgaa aatctaagaa aaacagacta
    17221 ttctatggtt gttcccgttt cccagaatgt gaattcactt cttgggataa accagtgggc
    17281 agaaattgtc caaaatgtga cggatatctg gtcgagaaga aagtcaaagg cggtaaacaa
    17341 gtagtctgtg taaatggaga ttacgaagaa gacatccaaa aataatagaa aagaggttct
    17401 ttcattgaaa atggtaaatg ttgttggagc agggcttgcc ggaagtgaag ctgcttggca
    17461 gatcgcacaa gcaggagtcc ctgtccgttt atatgaaatg cgtccagtca aatcgactga
    17521 ggcgcatcac acaagtaatt ttgctgaact agtatgttcc aattcattaa gaggaaacag
    17581 tttggctaat gcagtagggt tattgaaaga agaaatgcgc cgattgaatt cggtagtaat
    17641 cacttcggca gatgaaacag cagtgccagc tggtggtgcc ttagctgttg accgcgattc
    17701 tttttctgaa acaattaccc gaaaagtcaa agagcatcca ttagtaacag tgataaacga
    17761 agaaattact gaaataccgg aaggaatcac cgtagtagca acaggacctt tgacttctca
    17821 tccattagca gaaagcatca aagcattcaa tggttcagat ggtttttatt tctatgatgc
    17881 agctgcacca atcgtagaca aagctacgat cgatatggat aaagtatatt tgaagtcccg
    17941 ctatgacaaa ggagaagcag cttacttgaa ctgtccgatg acagaagagg aattcaaagc
    18001 tttccatgag gcactaatca atgccgaagt ggcagaactt aaatcttttg agaaagaaaa
    18061 atattttgaa ggatgtatgc caattgaagt aatggcacag cgaggattca aaacgatgct
    18121 tttcggacct atgaaaccag ttggattgga agatcctaaa actggcaaac gtccttatgc
    18181 tgttatccag ctgcgtcaag acaatgctgc agcttcttta tacaacatcg tgggctttca
    18241 gactcacttg aaatggggcg agcaaaaacg tgttttccgg atgattcctg ggttagaaaa
    18301 tgctgaattc gttcgctatg gtgtgatgca ccgaaacagc tttatgaatt cgccagaatt
    18361 gctgaaaccg acttaccaat caacgaaacg cgaagatctg ttctttgcag gtcagatgac
    18421 tggtgtagaa ggctatgttg aaagtgcagc gagcggacta cttgctggaa tcaacgccgc
    18481 acgcttagca aaagaaatgg aaccagtcga gtttccacaa gaaacagcta ttggaagtat
    18541 ggcttactac attactcatg cggaaggaaa acatttccaa ccgatgaatg ccaactatgg
    18601 tctgtttcct gaactgccag agcgcattcg agacaaaaaa gcaagatatg aagcaattgc
    18661 acaacgagct ttaaaagcta atgaggcaat aaaaacagaa ttagaaaaaa ctgcaatccc
    18721 aaatgagtaa ggaaactcca tgaagagggt gagacggata gtctcgctct ttttcttgcg
    18781 tttttcttaa gaatttctca aacaaaatgt gaggaattgc gccaattgtg aacaaactta
    18841 gaaatattgg ttaaattctg acaatttatt tgtcttttta taaacgctgt gctaaattat
    18901 tctctgggac aaaaggaagg tgagaaaatg acagaagcca cgtgggaaca aaaatttttg
    18961 cgttatttga ttgtcgaaag aggatattca gaaaaaacga aagaagcata tgaagaagac
    19021 attctccatt tcaaacattt tttggaggaa tctggagacg ctgatctgct gaaggcggaa
    19081 tattttacgg ttcgtgtcta tttgagtgca ttagctgatc atcgtcctag ctatagcaga
    19141 aattctatca gtcgcaagat agccagccta cgttcgtttt atcagtatct tctcaaagaa
    19201 gaagtgataa aagaaaatcc attttcttac gttcatctca aaaagaaaaa tttgagactg
    19261 ccacgttttt tttatgaaaa tgaaatgcaa gcactttttg acagcgtagc aggagatagt
    19321 cctttggatt tgaggaaccg cgctttactg gaagtccttt acgggtcagg catccgttta
    19381 agtgagtgta gcagtttgtc ggtatcagac attgattttg attcagaggt catgttgatc
    19441 catggtaaag ggaacaaaga gagatatgct cctctaggtt cttttgctca agatgctctg
    19501 caagaatatt tcagggaagg ccgccaggta ttgatgacaa aatatcacga agagcatgat
    19561 atcgtttttg tgaatcatca tggaaaacca atcacgccta ctggaattga atatgtcttg
    19621 aatcaaatga tcaaaaaaag cagtttggat agtgatatcc atcctcacat gctgcgccat
    19681 acatttgcta ctcatttatt aaataatggg gcagatttgc ggacagtcca agaattgcta
    19741 ggacatgcca atttatcgac tacccagatc tatgcccatg taacaaaaga aagtttacaa
    19801 aaaaactatc gttctttcca tccaagagcg taaaaataag gaaaagaaca aatttaggag
    19861 gaaacaaaca tggtcgaatc accatttcat tcaaccacta tctgtgccgt cgaaaaagac
    19921 gggaaatttg ctatggctgg agatggccaa gtaacaatgg gtgaatctgt tgtcatgaaa
    19981 ggcactgcaa agaaagtacg acgtatctac aatggagaag ttgttgtagg tttcgccgga
    20041 agtgttgcag atgcgtttac tttagaagaa aaatttgaag gcaaactaaa cgaatataac
    20101 gggaatctac aacgcgcagc tgtcgaatta gctcaagaat ggcggacaca gcaatctatg
    20161 caaaaacttg aagcgatgct gatcgtaatg aatgataaag aaatgcttct tgtttcaggt
    20221 acaggagaag ttatcacacc agatgatggt atcttagcta tcggatcagg agggaatttt
    20281 gcgttggctg cagcccgagc aatgaaaaac tatgctcaaa acgacatgag tgcgaaagaa
    20341 attgcagaaa atgcgttaaa tattgccgca gatatctgta tctttactaa tcataatatt
    20401 atcgtagaag aactgtaaga ggtgagtaga tatgtatgaa ttaaaacaaa caccaagaca
    20461 aatcgtaagt gaattagatg aatatatcgt aggacagcac gcagcaaaaa aatctgttgc
    20521 cgttgctttg cgtaaccgct atcgccgttt gcagttagat gaaaaaatgc aacaagatat
    20581 tacaccaaaa aatatgctga tgatcggacc tacaggggtc ggtaaaacag aaatcgcacg
    20641 acgtttggca aaaatcgtca atgcaccatt tgtcaaagtc gaagcaacaa aatttactga
    20701 agtcggttat gtcggaagag atgtagaatc aatggttcgg gatttagtag aaaatgcgat
    20761 ccaaatcgta gaaaaagagc agtacagccg tgtatacaca agtgctaaaa aaaatgcaga
    20821 aaaacgtttg gtaaaaatct tggtacctgg tatcaaaaaa gaacagaaac aatccactaa
    20881 tcaatttgaa caaatgatga atctttttaa tgctagtcag caaaataccg ctgaaccgca
    20941 agaagaagtg acagaagata ttcgaatcaa tcgccgtaca gtcttggaac aactacaaaa
    21001 gggtttactg gatagtcgtg aagtaaccat tgaagttgaa gaccaaaaga aacctgcacc
    21061 aatgatgaac aatggattgg agcaaatggg gatcgaccta aatgaaactc taggtgcttt
    21121 gacaccgaag aaaaaagtgg aacggacggt aacagtcaaa gaagctttag agttattgat
    21181 caaggaagaa tcagcaaaat tagtcaagga tgctgatatc catagtgagg caatcaaatt
    21241 agcggaagaa agtggaatta tttttatcga tgaaatcgat aagatcactt ctaaatcaca
    21301 gcaatctgga gaagtatcca gagaaggcgt ccaaagagac atattgccaa ttgtcgaagg
    21361 ttcacaagta aacacaaaat acgggatcat ccaaacagat cacgtcctat tcattgcttc
    21421 aggagctttc catttgtcta aaccaagtga tctaattcca gaacttcaag gacgcttccc
    21481 gatacgggta gaattagatg atttgacagc tgaagatttt gtgaagattc tgaccgaacc
    21541 aaataatgct ttagtaaaac aatacgtcgc gttgatcggt acagaaaatg tgtcagttac
    21601 gtttacaaaa gaagcaattg aacggatcgc tcaaatcgct ttcgatgtca accgtgacac
    21661 agataatatc ggcgcacgca gactgcatac cattttggaa aaactactag aagatctatt
    21721 atttgaagca ccagatatgc aaatgggaga aataacgatc acagaagcat atgtggatga
    21781 caaacttggc tcaattgcgg caaatgaaga tttaagtcgc tatattttat aaaaaatcgg
    21841 aggacgacaa tgacagactt gctaacgaaa acccgcaaga taaacacact tttgcagcaa
    21901 aagaatactt ttgacatgca agcagatttg ccttacgata aaatggctgt cacactaggt
    21961 ggtattcttg acagcaacac ctatatcata ggaagtgacg gagtattact aggttataat
    22021 gaacgtcacg atgtgaacaa tgaacgaatc aaaacaatgt ttgttgagaa acaatttcct
    22081 gaatcttata ctgatacagt ggcacagttg atgaaaactg aagctaatat cccaattact
    22141 agtgatatta ctgcatttcc ggtagagtca agagagaaat atccatttgg tctgacgact
    22201 gtcgttccga tctttggagc tggcgaacgt ttgggtacga ttatcttatc tcgtatggaa
    22261 caatcgtttg cagatgacga tctagtctta gcagaatatg gagctacggt tgtcggtatg
    22321 cagatccttt atcagaagtc tcgaagcatc gaagcagatg tacggagtgc aacggctgtc
    22381 caaatggcaa tcaacacatt atcttatagc gaattgaagg ctgtacaagc tatctttgaa
    22441 gcattggatg gagatgaagg tcgtctgact gcttctaata ttgccgatag tatcggcatc
    22501 actcgttctg tgatcgtcaa tgctttacga aaattggaat cagctggaat cattgaatct
    22561 cgttcgttag gcatgaaggg aacttattta aaagtattga attcccgttt taaagacgag
    22621 ttagcaaaac acagttatta gaagtaaggg gactggggca gatagaaatg tttgttccag
    22681 ccttcccatt ttaaaaagta agtcgaagtt ggaggaatgg caatgactgt tattattcaa
    22741 aatgaccagc tgatcgccga aatcgctgaa cacggtgcgg aattgatcag tctgaaatca
    22801 aaagaacatg gtttagagta tatctggcaa ggtgatccag cgtattgggg cagacatgca
    22861 ccggttttat tcccttttgt tggacgttta aaagatgatc agtatgttta tcaagggaaa
    22921 acatatcaaa tggggcaaca cggatttgct cgggatatgg attttgaagt cattgaacat
    22981 ggacaagaaa tggctagttt catcttaagg agtacgccgg agacattaga aaagtatcca
    23041 tttgattttg aattagtgat cagctatgaa ttaggaggag acggcatcac ggttcattat
    23101 caagtggcaa ataccggtga aaaagaaatg ttcttttcta tcggtggaca tcctgctttc
    23161 aatgtacctc ttgaagaagg attgtcattt gaagatttct atctggcatt ttctccattg
    23221 aaatcaagat tacgcctacc attgaaaggc ccattcatcg atatggatca aaagacgtta
    23281 ggtcagacaa atacgaatct ggctttgaat cgtgaacttt ttgagcaaga tgccttggtt
    23341 tttgaaacaa ggggtctaaa tgcgtacgcc attcgaagtg aaaaatcttc ccatagcgta
    23401 actttaagct ataaagatat gccttatgta gggatctggt ctccttatcc aaaagaagcg
    23461 cctttcgttt gtatcgaacc gtggtgcgga atcgctgaca cggtgaacag tagtggaaac
    23521 ttgatggaaa aagtagggat caatcaacta ggtgcccatg aaatcttcaa gacaaaatat
    23581 tccatcacag tgaagtaaga ggttgtgacc gagaagttag cagggaacac cgtttaccaa
    23641 ggtgtgtaaa aaagctatcc tgtaagtaat aagggagtgt gacatcgatt tgtggctctt
    23701 tgtcaataag gactgatgag ttgtgcaa
//
LOCUS       DACCCS010000285        12790 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.guided.077, whole genome shotgun sequence.
ACCESSION   DACCCS010000285 DACCCS010000000
VERSION     DACCCS010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.guided.077"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..515
                     /locus_tag="IVK82_003089"
     CDS             <1..515
                     /locus_tag="IVK82_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP6394064.1"
                     /translation="LAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQF
                     VYAAGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKEL
                     EIIEASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLE
                     AALKEILDQE"
     gene            complement(766..2724)
                     /locus_tag="IVK82_003090"
     CDS             complement(766..2724)
                     /locus_tag="IVK82_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6394065.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2721..3593)
                     /locus_tag="IVK82_003091"
     CDS             complement(2721..3593)
                     /locus_tag="IVK82_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6394066.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3935..4807)
                     /locus_tag="IVK82_003092"
     CDS             complement(3935..4807)
                     /locus_tag="IVK82_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6394067.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4935..5504
                     /locus_tag="IVK82_003093"
     CDS             4935..5504
                     /locus_tag="IVK82_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP6394068.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5571..6308
                     /locus_tag="IVK82_003094"
     CDS             5571..6308
                     /locus_tag="IVK82_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6394069.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            6539..7807
                     /locus_tag="IVK82_003095"
     CDS             6539..7807
                     /locus_tag="IVK82_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP6394070.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            8294..8758
                     /locus_tag="IVK82_003096"
     CDS             8294..8758
                     /locus_tag="IVK82_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6394071.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8742..9371
                     /locus_tag="IVK82_003097"
     CDS             8742..9371
                     /locus_tag="IVK82_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP6394072.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            9725..11203
                     /gene="msr"
                     /locus_tag="IVK82_003098"
     CDS             9725..11203
                     /gene="msr"
                     /locus_tag="IVK82_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP6394073.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            11250..11696
                     /locus_tag="IVK82_003099"
     CDS             11250..11696
                     /locus_tag="IVK82_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP6394074.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11895..12410)
                     /locus_tag="IVK82_003100"
     CDS             complement(11895..12410)
                     /locus_tag="IVK82_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6394075.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            12527..>12790
                     /locus_tag="IVK82_003101"
     CDS             12527..>12790
                     /locus_tag="IVK82_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6394076.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKK"
BASE COUNT         4086 a         2344 c         2588 g         3772 t
ORIGIN      
        1 tattagcgcc agatcaccaa ttcactgaaa aaatgattaa ggattcaggt atttcccata
       61 catttttacg gaataattgg tatgtagaga atgaattacc tatcattggg caggctttaa
      121 aaactggtca attcgtttat gctgcaggaa atggaaaaac aggttgggca ttgaaacgtg
      181 aatatgcaga agctgcagca aaagtactag tagaaagtga ctctccagaa atcttagaat
      241 tatccggcaa tctaactgac tatgaagaat tagccaaggc attagagcgt gcgacaggta
      301 aagagttaga aattattgaa gcaagtgatg cagcgtttgt cgaaaatcta aaagaagccg
      361 gattccctca agaagcagct gacatgtttt tagcgatcca acatgatatt aaaaaggatc
      421 agttagctat ttcctctgat gatttagaaa aagtattggg aaaaccatta acttctcttg
      481 aagcagcatt aaaagaaata ttggatcagg aataatgtat aaagaggttg tgaaaaagcc
      541 gattagtttt gagtatgaag aaaaattttg tgaaaaagct gttctgcatc aagtaataag
      601 aagagtcaaa taaaaaatag ctcttcatat gtttcgttta gctggactta ttaccgcgga
      661 gttggcagga gaacgccgtt tttattcgga aaaaggagct gagacaagat ttttgtctca
      721 gctcctgttt gtttatcgat aaaaaagtct ggggaagacg atggctcagg ctttgttgga
      781 aacagagccg attttatcat ttgcttttac atcacgtgcg ggttcgattt ctaagctttg
      841 gactttgtcg ccatttgtga atacaacgac catatccgtg tttttgcctg cagattgaat
      901 agcggcaaga tcaactaaag caatcaattg acctcttgcg actttttgac cttcttctac
      961 atataaagtg aatggttcgc cttttaagtc aacagtgttg atgcccatat gaagcaagac
     1021 ttcgatacca gcatctgttt ggattccgac agcatgtttc gttgggaaaa tgtttgtgat
     1081 cgtaccagcg attggagaga agatttctcc attttctggt aatacagcat agccgtcacc
     1141 catcattttt tccgcgaata catcatctga aacttcagtg attggaatca gtttcccatt
     1201 tgcgatagcg taaatatctt gttcgatcgt ttcaccggca aatgtttctg ttttaaccac
     1261 ggtgtttgtt tgtgcaggtc gtgctgcagc accaccatgt agttttgcca tctcatctgc
     1321 aacgaattgt acttctgtac ctacgatgac ttgcgcattt tttccgtcga tgatcttcat
     1381 accaggtacg ccagtggctt tgattttatt ttgatcgatc aggcctgtat ctttgacttg
     1441 caaacgaagg cgtgtagtac agttatcgat cgaagttaca ttgtcggctc cgcctaatgc
     1501 agcgtagatt ttgcttgctt gtgccgcata tttattgtct gaattagtcg ttgcagtatc
     1561 gtcatctgaa agatctgctt cctcgcggcc aggagtcatc agatggaatt ttttgatagc
     1621 aaagttaaag ccaaagtagt aaatcactgc tgttactaat ccttggacga tcaacatcaa
     1681 tggctgattc gcaatcggat ttttcaaaga taggaagtaa tcgacaaatc cggcactaaa
     1741 ggcaaatcct gctgtccaat ggaagaatgc actcactgct aaagaaatcc cagtgaaaat
     1801 tgcatgcaag acatataatg gccaagcaac gaacataaat gaaaattcaa gtggttctgt
     1861 cacaccagta aagaatgaag caaaagctgc tgctagcatc aaggaagctg tttttgcttt
     1921 gttttctgga cgtgcatttc tgtaaatagc atatgctcct gctggaagcc cgaacatcat
     1981 gattgggaaa aatccagctt gatacattcc tgttacgcct tttgttcctt cagaagccaa
     2041 aaatttacca atatcattga ttcctgctac gtcaaaccag aacacggagt tcattgcttg
     2101 atgcaatcct gtagggatca gtaaacggtt gaagaagcca taaagacctg caccacccca
     2161 gcctaaactc aaaatgaatt tagcaaaaga aactaaagca gagtaaaccg gaggccatac
     2221 gagcagtagc actgcactga tcagcaacat gacaaaggca gccaagattg gcactagacg
     2281 cttgccgcta aagaaagata aagccatcgg taattttgct tcgtggaaac gattataaag
     2341 agctgccgca atcaatccgg caataatacc aataaatacg ttactgatct gagcaaaaga
     2401 tggatcaact gattctggtt tgatattcaa taaagtagct actgaatcag tttttaaaac
     2461 atttactgga acttcaaatg cgaccaaacc tgccaaggca gcagcacctg atttgtccac
     2521 tgacatccct agtgctaacc caacggcaaa cagcaaaggt aattgaccta aaactgcgtt
     2581 accgccagct tgtaaaaaat gagcgacaat gtcgttacta taagatgccc accagtttcc
     2641 gatccctact agtaaagcag cagcaggtaa gaccgcaact ggaagttgaa gggaacgtcc
     2701 cattcgttgt agatatgctt tcaatttttt tcctcctcat gattgttaac atttgtttgt
     2761 acgattgcgc ttcttaaacg ctcgatatgg atggccaagt aacccagttc ttctttcgtg
     2821 atcgcgagct ggtattcttt ggtcagcaac acacggattt ttttagaaat cttatagctt
     2881 tctgggtatt ttcgtttgac catttctacg atttccgtgt ctaaaacagc atattgttgc
     2941 gcatgggttc gttgtagaag cagtcgtaaa tgattcacca gtcttgaata attcagtgaa
     3001 aatggttctg aatggatatc tgtttccaat tctgattcca ctaaatggat gatgtcagat
     3061 acaatcgtca cttcacggat actgcgatgg ttattgttcc gaccgcttcg tccgctgtga
     3121 atatggattg cgatatatcc tgcttcgtca taagtatatg gaatcccaag ctcttttgtc
     3181 agatactcta ctgcccattg agcaatactg aattcatcac tgtaaagcac ttcgatttct
     3241 cttaaaagtt tgttgcggat gacgataccg ttattaatgt tctccgcaga aaaagcaatg
     3301 tggtcggtca aagcgatcaa tacatgttca ttcaactttt ccatcaaaac agtttccgca
     3361 tgatcaatga tcgtttctgc tgcaaataaa aagcgttcat caatctgatt aagtaaattt
     3421 tgcaacttga tttgaccttc tggttccatg acaaatatcc gttcgacatc acgatcaaaa
     3481 atcaaatcat ttctttttct gtcaaatcca atcccttttc caatagctac tttttcctgt
     3541 ccaccgtcat ctaccaatac tgcattttga ttaaagatct ttttgatttt catgcgcgca
     3601 ccctttttga agatttggtt ttcacgtttg ctagaccagt tgattgcggc caaacaaaaa
     3661 tagacacgaa aaacctccta gggaaaccat gttcctcaga gatttttcat gcctagtatg
     3721 atctagtcac atgaaacgaa ttattgttaa cgtaatcaat tttatataga cagttatgtt
     3781 ttgtcaattc tttttttata attatagtaa tttactggaa ttatcagaaa taaaaggaag
     3841 aaataaatta agaaacttat gttatttaat cgttggatct tttgcttatc ttattcgttg
     3901 ttaaaaagct gaaagcgaag gaaaaaaact ctacttaact gtatttctcc gttaggacat
     3961 ctaaaaattt acgagcagca ggagaaagag gctggtcttt tttccaaatg agattcagtg
     4021 aagcttgtaa tggcggatcg agtgggatga atattttttc cgtcccataa gtattgatga
     4081 tcccatctag agctaatgca ccaaaagtcc cgttatcgac catcaaagaa gcattgtaca
     4141 ataagttata tgttccgacg atcgataact gatctatgct gaaacctagc cattcagcta
     4201 gttgactgtc cactgacgtt tgccgggaaa tgatcaaagg aacagttttc agttcctgcg
     4261 gatggatgac ggtttgcttt gctaaaggat gatttcgatg tatcaaaagt ccccaacgat
     4321 cttgggctgg cagtcgaaat gactggtatt tttgtttgtt tgtcggttcg attacgagtc
     4381 caaaatcaag taatccgcta tccagtttgt ctatgatatc atctgcattc ccgctatata
     4441 aatgaaactg gatatcagga tgcgccttga tcaacgaatg aaggctatca gaaatgaaag
     4501 agattgcttt agtttcgcct ccgccaatgt agatttctcc agagatgatt gcgtcatttg
     4561 ttaaattgtt cgccgttttt tctaccaacg acaagatttc ttttcctttt gaataaagat
     4621 atcgaccaga atccgtcaac tgtatctctc gggagccccg agtgaaaagt gtcacaccaa
     4681 gttcttcttc taaatctttc agttgttttg agagagtagg ttgcgtgata tgcagagatt
     4741 ttgctgctcg gctgatattt ttttctctag ctactgctaa aaaatacgtc agtatacgaa
     4801 tatccatttt ttccctcctg tttttaaaaa agcataccac taagtatagt taaaaggaat
     4861 agttaagtat gttttataag tattagacat gataagccgt ccgttataaa gtaaggataa
     4921 aaggagggag caaaatgaca agcctcatcg aaaaacaaat cgtcaataaa gagattcgag
     4981 taacggatcc gctctttgaa gtgatccatc agatccaagc tgaaaacgaa gagcctttag
     5041 cagcgctgaa tactgggtac catgaaccag cagatatccg caaacggcta gaaaatatca
     5101 ttagtgacaa agtagatgat acggtgactg ttctgctgcc tttttataca gatttcggca
     5161 aacatattag tatagggaaa aatgtcttta tcaatcgtca agtgatgttc gttgacttag
     5221 gtggcatctg tttagaagat tcagtattga ttggtcctcg tgtcaacctc atcacggtga
     5281 accatctaac cgatccaaaa gaacgaagag gcttgtcagt aaagccaatc cacatcaaga
     5341 aaaatgcttg gatcggagca ggagcaacta ttctgccagg cgtaacgatt ggcgagaatg
     5401 ccattgttgc agcgaatgca actgttacga aagatgtccc agacaatacc atcgttgcag
     5461 gaatccctgc caaaatcgtc aaacctgtcg aacggattat ttgaacagta agatgaaata
     5521 agagatagtg agtataagca agagattttt caaaacagga ggagaataga atgaatgatt
     5581 tatcagaaaa agtgattgtc atcatgggcg cttcaagcgg aatcggtgaa gcaacaacga
     5641 agctattagc agaaaaaggg gctaaactag ttatagctgc acgccgagaa gatcgcctaa
     5701 aagcaataaa agaatcttta ccagaagctg agctatatat tcaaacagca gatgtaagag
     5761 attttgcaca agtccaagca gttatcgatt tagcgatgga aaagttcggt cgaatcgatg
     5821 tattgtacaa taatgcagga atcatgccaa ccgctccatt agttgaaggt caccgtgatg
     5881 aatggcaaaa tatgcttgat atcaacatca tgggtgtgtt aaatggaatt tccgccgtac
     5941 ttccaatcat ggaaaaacaa aaatctgggc atatcatttc aactgattct gtggcgggac
     6001 atgttgttta tcctgattcc gccgtttatt gcggaacaaa atttgctgtc cgtgcaatca
     6061 tggaaggatt acgtcaagag caacggcaaa ataacatcaa atctacgatc atctctccag
     6121 gagctgttca aactgaatta tatcaaacca tcagcgacaa aaaggctgct cttgaacttc
     6181 atgaagcgca aaaagaatgg ggattgacct ccgaagatat agcttctgct gtagcatttg
     6241 cgatagaaac gccagaccgg atgtctgtca gtaacatgat tatccgtccg acgacacagg
     6301 aagtataatc cataacggat aatatccaaa gagaattact tgttaaatag ttattcaaac
     6361 gaccatcaat ttaaggcgtg aatggagaag aatattcttc atttacgtct ttttatgtag
     6421 aaaaagcaaa tttatcaaaa agaaacaaat gttatttaaa tatggacatt gacttgcttt
     6481 tactttttta gttacactgt tatttaggaa aagtttcata attcaaagga gaaaaaagat
     6541 gaaagcaaag aatttattat ttagcgttat tcttttagga aatctagcag ttgccaacac
     6601 aacagtgcta gctgaagaat cgacagaaac tgtcgcaact cagacatcga gttcttcaga
     6661 aacaggaatt gaaaacgata cagagagtac tttgcagtca gctactgtct cagaaaatac
     6721 aatttcaacc gaagaaccaa caactgaaac aacagaacaa acacaaaccg aagacagtac
     6781 atcagcagaa gctcagaaca atccagagaa tgcagaagaa atcacgaacc aattgacaga
     6841 tgggcaaaca ttgacacata cccatcaagc agatttgtct gaattcgaaa actatactgg
     6901 ctatgctttt tatgctcgct catctgctaa ttctcaacaa gcgttcattg atagtattgc
     6961 ctcaactgca caaagtttgg caagtgccaa tgacttatat gcatcagtca tgattgctca
     7021 agccatcgtt gaaagtggct gggggaacag tactttggct tctgccccta attacaatct
     7081 atttgggatt aaaggaagct ataatggcca atctgttacg atgcctactt cggaatatgt
     7141 gaatgggcaa tggattacgg tgaatgccgc ttttagaaaa tatccttcgt acaaagaatc
     7201 attgcaagat aacgtaacgg tattgaagac gacttctttt cagccaggcg tttactacta
     7261 ttccggtgca tggaaaagca atacgaactc ttacaaagat gctacggctt ggctaacagg
     7321 tcgttatgcc actgcgccta attatggttc tacattgaac aatgtaatcg aaacctataa
     7381 tctgacacag tatgatacag caccaacttc aacttctccg atgtatcgtc tgtataaccg
     7441 tcatacagga gaacatttat atacattgaa tgccggagaa aaaaattatc tgccaactgt
     7501 aggctgggaa tatgaaggga ttgcttggca agcaccaaac agcggacaac ctgtctaccg
     7561 tttatacaat ccttacagtg gagaccatca ttacacaatg gcacaatctg agatcaactc
     7621 tttaacaaaa atcggttggc gttatgaagg tctaagtttt tattctggtg gttccaagcc
     7681 aatttatcga ctatttaatc caaatgaaaa aactggtacc catcactata ccttgagtgc
     7741 taaggaaaga gattggctaa caccgatggg ctggagatat gaaggaatag gattttatgg
     7801 ttattaaaaa gaggtagtga aaaagctgcc ctgcatcaag tagtaagaac agccaaacaa
     7861 aaacagattt tcatgttttt tgtttggttg aacttattac cgcagatgca agtttttgaa
     7921 caccgtttac tcaaaaaagg ttgtgacaac atcttgtcat aactttttta ttactgttta
     7981 agcatactga tccaatttct aatattcgac gggattcttg caaaaggcga tttcggaaat
     8041 ttttggcggt gtaaaatttt ctactgcaga agagctatct tctatagttt gataagaatc
     8101 aataatcttg gttttagcgt tgtgctttct ttatcatttt ttccgttcat tctacaaaaa
     8161 atttcttttc tctatacttt tctattgaga gcgttttttt tattacacag ctgattcttt
     8221 gacttttttc tttttttatt ctattattga caagtcaatc gttgacgagt caattattag
     8281 aaaggaggaa attatgtctt tagaagagat tagtcacctt ttatatcaaa tcaaattagc
     8341 cgatcaaaaa atcgtccaga tctttgaaga aaatatcggt ataagtctta ctagatacga
     8401 gatcatgatg agactgaaag aagaaaatcc attgctacaa agcagtttgc aagaaaagtt
     8461 gcagattgat caaggagcag ttactcgtca tctgaaggta ttggaagcaa atggctatgt
     8521 ttcgcgtcag agaaatcctc agaacaaccg ggaagtgtat gtagcgctaa cagaaaaagc
     8581 ccagaagaaa atcgagcatt gtgaaatgga ccataaagat acaggttccg tgctatcccc
     8641 ggctttcagc gatgtagaaa tgaaacaatt tctaacactt ttagataaat tgaataccca
     8701 actttgcaat ataacaacag aaacgaaaga ggttaataat aatggaacaa aaaaatgatt
     8761 tttcaacaat tatgactggt agaagatccg ttcgcgtata tgatgaaaca tacaaaatcc
     8821 cacacgaaga aatgctggaa atgatcaaag aagcgacgac tgcaccttct tctgtcaata
     8881 tgcaaccatg gcgctttgtg gtcgctgaaa gcgaggaagc aaaagaaatc ttgcgtcctt
     8941 tgatccgttt caatacgaga caaaatgata cttcttctgc aatggtcatg atttttggcg
     9001 atatgcaatg ttacgaatat ggcgaagaaa tctataacca agcttacgaa tcagggaaga
     9061 tgccaaaaga agtcagagat caacaattag ctgcgatcat tccttattac aagagttttt
     9121 caagagaaga aatgaacgat gtagtcaaag tcgattctag cttagcggct atgcaattca
     9181 tgttagttgc gcgtgatcat ggctatgaaa caaatccaat tggtggattc gaagcagatc
     9241 agttggcaga ggcatttggt ttggataaag accgttatgt tccagttatt attttatcaa
     9301 taggaaaagc ggtagaagaa ggttacgagt ctgttcgttt agcaccagaa aagatcacta
     9361 cgttcgaata aaaggttttc agttactatt cagtagctat tgcttttttt gaatcgtgtg
     9421 tggtaaccta ataaaaaagg aggtcggaaa aatgactgca tcgatgaaat tacgtttcga
     9481 acttttgaat aacaactaaa taaattcaaa agacatgtat aaacaacaat ttcatggatg
     9541 gagtatgtcc attttttcga acgacagtag aataggtgaa agctatgaca gatttggtga
     9601 tttagtcata gttatttcgt tcagagtagg aaaagatgtg atgcagtttt tttgcaaaca
     9661 gatttttctg ctctttttta gtatcgataa ttgttgtttt acatgaaata ggagtgtaaa
     9721 acatatggaa aatttagcag taaatataac aaatctacaa gtcagctttg gcaaccagct
     9781 agaactatct attgattctc ttcgtgtcta tcagcaagat cgtataggga tcataggaga
     9841 aaatggtgtc ggtaaatcga ctttgctcaa actaatagcc ggtgaacttt ttcccgatca
     9901 tgggaaaatc caaacagaga tcaccttcaa ctacctgcct caattaacct atctcgctga
     9961 agcaaaggac ctaaatttgg aattagccag tcatttccag ttaagactgg aagaaacttc
    10021 agagcggaaa tggagcggag gggaagaacg aaagatcgag ttgatacgtc ttctttcttc
    10081 ttatgaacaa gggatgcttc tagacgagcc aacaacccat ctagatagaa aaagtattga
    10141 tcgactgatt gaagagcttc gttattatta tggtacgctg gtttttgtta gtcatgatcg
    10201 ctattttcta gatgagctag catcgaaaat ctgggaagta aaagacggag aaatccgaga
    10261 gttttcgggg aattatagtg cctatctcac tcaaaaggaa ttggagaaaa agactcagct
    10321 acgagaagca gagtcgatca tgaaagagaa aaaacgattg gaaaaatcga tccaagaaaa
    10381 gaaaaaacaa gcggaaaagt tggaaaaagt gtccagtaaa aagaaaaagc aacaaatcag
    10441 accggatcgg ttgtcttcct ctaaacaaaa agacagtgta caaaaagcca tccaaaagaa
    10501 tgcgaaaaca ttagagagaa gactccaaaa aataggagaa acaaccaagc cgcagcagat
    10561 gaaacaaatc cgttttccag taccaaaatc tcttgaactc cacagccgct atcccatcat
    10621 gggacaaaat gtccaattgg aaagaagtgg gagaacatta ctggtaaatg gtgattttca
    10681 gttttcttta ggtaaaaaaa tcgcgattgt cggtgaaaat ggttcaggta agacaacctt
    10741 attagaacat atccgcaaac aaggagaagg aatccttctc tctccgaaag taagctttca
    10801 agtatatcag caaaagggtt atcaaatgac atctgaagaa tccatcattc gttttgtcat
    10861 gagacaaaca gagttttcag aatcgcttgt ccgtagtttg ctgaatcact tagggtttgc
    10921 tcaggaaact ttgacgaaac cgttatgtac attaagtggg ggagaagcga cccgtctgac
    10981 gattgctttg ctttttacta agccaagtaa tgtgttgctg ttagatgaac cgactaattt
    11041 tattgatatg gcaacgatcg aagctttaga gaagctaatg caaatatatc cgggaacgat
    11101 tttgtttact tctcatgatt cttactttgt cgagcgtacg gctgatgaag tttatgaaat
    11161 aaaagggcag aaaataaaaa aagtacttac gagaaatttt taaatgtgct acgatgattt
    11221 tcgtgacaaa tttaaaggag tggtacagat tgcctacaaa taaaaaagaa ggaataattt
    11281 ttacaacaat gatgtgtttt ttgatggtct tggggatgag cgcgtataat ttatggctgc
    11341 atcaagatct tcatttatca agtttgttga ttggtctagt gcctggattc gtggtggctt
    11401 tcattttgga tgtcttcatc atcggtgtgc tggcgaaaaa aattgccttt aaactaccag
    11461 taaatccaga aagcaaggtc caaatgatct taactatttc atgcttgatg gttttaggaa
    11521 tggtgacatg tatgtcttta ttcggtgtga tcatggaaca aggaatacct gatcaattat
    11581 tctctgctta tatgaatgct tggaaaatga atgtgattgc cgctttaccg ttgcagttga
    11641 tcatcgttgg cccgctttca agaagtgtgc taaaagcaat tcaaagtaat gaatgatgga
    11701 acactgttat ttgaaaaaag gccttggcat gctttttgtc atagtctctt gaatgcagtt
    11761 ctagaaaaat agtgatccga acgataggtt ttggcctgtc gttcggatcg ctttttcttt
    11821 tccaactgta ttgctctttt attgaagata atttgctctt caatagatat ttctagaaag
    11881 gttctgttta tcccttactc aaaaaactgc tgcatttgtt ctcttacttg ctgacaccat
    11941 tggagttcct gctgacaaag cataattctg cgctggatca caacataacg tccaaattct
    12001 tgtttcttgt ctagcgagag gtcattcaat tttttttgat ttttttgcat gattttgttc
    12061 agctgttgtt ctctagtgaa tagcagtcca gtagctgttg ttttatcgag cgtcgaaaaa
    12121 gcatagattt ttgctagaaa ttcatctttt tgcgtaggag cgggagtttc ctctttgatc
    12181 cattcctcta caagagcacg tccttcatca gtcaagtggt agatcttttt ttgagagtgc
    12241 tcgtctttgt ccaaaacggt cacataaccc tgctggtcta acttggcaag cgttgtatag
    12301 atctgactgt gatgggcttc ccaaaaaatt gtgatcagct gcttcaattc atacccagaa
    12361 cgtggtttgg ataaaagcac gcataataat atataggcta aagtattcat aaaaatccta
    12421 cctctcgtct tattttcttt attatatcac agtaaagttt ccacgtttta catgtgcata
    12481 tttacatgta aaataaatca tgtatgatgc taatagagag aaagggatgg aaatattgga
    12541 gaatcaaata gatatacaag aacaaacaaa tgaagaaaca aaggggaaaa gttcagcgct
    12601 agttgtagca gctacctgta tgatcgcttt tatgggagtg ggtctagtag acccaatctt
    12661 aaaaacgatt gctgcacagt taaacgctac gccagctgaa acgaccctgt tatttactag
    12721 ttacatgtta gtcactggtg tggtgatgct atttacaggt ttcttatcta gtcggatcgg
    12781 tttgaaaaaa
//
LOCUS       DACCCS010000286        11626 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.guided.080, whole genome shotgun sequence.
ACCESSION   DACCCS010000286 DACCCS010000000
VERSION     DACCCS010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.guided.080"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            26..601
                     /locus_tag="IVK82_003102"
     CDS             26..601
                     /locus_tag="IVK82_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6394077.1"
                     /translation="MIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLDKQAGKIAQ
                     QIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKIEQADVIIT
                     GEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPI
                     GLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            859..2361
                     /locus_tag="IVK82_003103"
     CDS             859..2361
                     /locus_tag="IVK82_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP6394078.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2428..2955
                     /locus_tag="IVK82_003104"
     CDS             2428..2955
                     /locus_tag="IVK82_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6394079.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3166..4227
                     /locus_tag="IVK82_003105"
     CDS             3166..4227
                     /locus_tag="IVK82_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP6394080.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4230..5126
                     /gene="murQ"
                     /locus_tag="IVK82_003106"
     CDS             4230..5126
                     /gene="murQ"
                     /locus_tag="IVK82_003106"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP6394081.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5140..7122
                     /locus_tag="IVK82_003107"
     CDS             5140..7122
                     /locus_tag="IVK82_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6394082.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(7187..7633)
                     /gene="nrdI"
                     /locus_tag="IVK82_003108"
     CDS             complement(7187..7633)
                     /gene="nrdI"
                     /locus_tag="IVK82_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP6394083.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            7844..9268
                     /locus_tag="IVK82_003109"
     CDS             7844..9268
                     /locus_tag="IVK82_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6394084.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(9449..10222)
                     /locus_tag="IVK82_003110"
     CDS             complement(9449..10222)
                     /locus_tag="IVK82_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6394085.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(10219..11124)
                     /locus_tag="IVK82_003111"
     CDS             complement(10219..11124)
                     /locus_tag="IVK82_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6394086.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(11278..11598)
                     /locus_tag="IVK82_003112"
     CDS             complement(11278..11598)
                     /locus_tag="IVK82_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP6394087.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         3727 a         2064 c         2514 g         3321 t
ORIGIN      
        1 aaacgttctt gtttctaaag taaaattgat tatcgctgca gatgttacca atccatatac
       61 aggtcaacaa ggagctgctt ggcaatttgg aagacaaaaa ggaggaatag atacgacgct
      121 agctttctta gataaacaag caggaaagat tgctcagcaa ataaaagatt tctgggaggt
      181 ggatttagat gatattccag gaagtggagc agcaggcggt attggtggag cccttttttt
      241 gttgggagcg aagatggttt ctggtttcga tttagtcagc caaatcactg gattggaaaa
      301 aaagatcgaa caagcagatg tcatcattac aggagaaggg cggatcgatc agcaaacgat
      361 ccacggaaaa gttccatacg gtgtagcagt gcttgcaaaa aaatatcgaa aaccaataat
      421 cgctatttgc ggaagtagag accaagagct agaaggactt tctgattttc tcccaattgt
      481 cttaagtatt catttgggtc caataggttt agaagaagca atggaacaca ggcaaacatt
      541 agaaaaaaca aaaatcttag gacagacact tagcagattt ttcaccatcc tcccgaagta
      601 gttgacaaaa tcattttctt tatgctatcg taatttcaaa tataggacaa agtgagggat
      661 ccgtgcattg aaaaattaag tcgattgatg aaagctgcat tcagactgta ccttgtggag
      721 acaaggaagc agtgtgcaga ttttcgggat tggcttggtt ttttcgatgt atgggtcttt
      781 ttgtgtgtat ccaacccttg catcctcttt agccagttct gaaaatagga ctggtctttt
      841 tttacgaggt gatggaaaat gtctaaaatc gaaataaaaa atctgacatt cggctacgac
      901 agccaaggca cattattatt tgaacaagca aatctaaatt ttgacacaca atggaaacta
      961 ggacttatcg gacgaaacgg tcgaggaaag acaactttac tgaatattct acaaaacaaa
     1021 ctaccttatc aagggcaagt aatccatcag caagaatttg cctatttccc gcaacagaca
     1081 aaagataaag aacgtttaac ctattacgtg ttaaatgata ttacggattt tgagatatgg
     1141 gaaatcgaaa gagagctcca attgatgcaa acagatcctg aaatcttatg gagagaattc
     1201 agcacactat cggggggaga gaagacaaaa gtcctactgg cacttttatt tgtggatgac
     1261 actcatttcc cgttaatcga tgaaccaacg aatcatttgg atatctctgg tagaaaacaa
     1321 gtagcggctt atttgaaaaa gaaaaaacaa ggcttcatcg tggtcagcca tgaccgggga
     1381 tttatcgatg aagtagtgga ccatgtttta gcaatcgaaa aaagtcaact ggaactttat
     1441 caagggaatt tctctatcta tgaagaacag aaaaaacttc gtgatgaatt tgaaatggct
     1501 caaaatgaaa aattgaaaaa agaagtcagt aggctaaaga aaacagcagc tgaaaaagcc
     1561 gaatggtctc gttcccgaga aggagataaa acaaagaaac aagtcggatt catcgatact
     1621 gaatctagac gagtgaataa aggagcagtg ggtgctgatg ctgcacggac gatgaaacga
     1681 tccaaagcaa tcgtgaatcg gatggagacc cagatcagcg agaaagaaaa actattaaaa
     1741 gatatcgaat atatcgattc tttgacgatg aatagccaag cgtctcacca taagcgactt
     1801 ttaagcgtag aagatcttca attagggtat gaaaatctgt tattcgagcc aattcatttt
     1861 acaatcgagc ctcatcagcg ggtggcgatt tcaggtccta acggtgcagg aaagtcatcc
     1921 attatccatt atcttctggg ggcattcaac ggcaaggtta taggagaaaa ataccagcca
     1981 aaacatctga gcattagtta tgcaagccaa aattatgaag acaatcgagg aacgttggcg
     2041 gaatttgcag agaaaaacca agtagactac caagcatttt tgaacaacct ccgaaagctt
     2101 gggatggaaa gagatgtttt tcataacaag atcgagcaga tgagtatggg ccaacggaaa
     2161 aaagtggaat tggctaaatc tttatcacag ccagctgaac tatatacatg ggatgaacca
     2221 ttgaattatt tggatgtctt caatcaagaa caattagaac aactgatctt gaacgtgaaa
     2281 cctgccatgt tactagtgga acatgatcaa accttcctgg ataaagtatc tactgagatt
     2341 atttctcttg agagaatcta acccttgtaa tctcaagaac aacaaggtat ggttaagggg
     2401 aactttgcca acgaaggaga gaatcacatg ctttatgtaa cgagacacgg agaaacaact
     2461 tggaacgcac aaggattagt ttgcggacgg gcagatgtgc cgctgactga aaaaggacag
     2521 atgcaggcac agaaactagc agaaaaggta gtagatttgc ctgttccaat cacgaaaatc
     2581 atccattccc ctcttcaacg agcaagagat acagcacaag cagttgctga tcgtctcagc
     2641 ttgccgctga ccgttgatga acgattggta gaaatggatt ttggggatta tgatggactg
     2701 ccaagcaaag atgagaactt tcaaaaagca cgtttagcgt tcgctgtacg attcccaaat
     2761 ggggagtcag tcttagatgt gtatgcacgg atcgttcctt tattgaaaga atgcatcgaa
     2821 gacgaagaaa atgtctatct gttagtttgc cataatgcct tgataagagt gatcaatgct
     2881 tattttcatc cgatgcctaa tgaggggttc tttacattca tggtagataa tacagaattg
     2941 gtttcttatg agtaattatg tttttgtgtt atttaaataa aatatttcaa ggcactgctt
     3001 ttgcttagca gtgccttttt taggttatga caattctttt gataatataa actcaacctt
     3061 tagaaatgaa atttaattat tttaataaaa gtattgaaat tttgtttctt tagatatata
     3121 ctctagtcat agaaatgaag acggatacag aaaaagaggg ataatatgtt tggcttttca
     3181 atatttatga acgattcttt gtcagaagag aagaaacagt atatccgcca aatggcatcc
     3241 aatggctttg tcggtatttt cacttccatg catataccag aagatgatgt ttcggcatat
     3301 caaaagcgat tgatcgaact aggaagcgaa gcgaaaaaga ataaactaga attgatggtc
     3361 gatatttcag gagaggcatt ggaacgagcg ggtttttcat tagaagatct aacgcctttg
     3421 aaagcaattg gtgtaacagg tttaagaatg gattatcata tttccaatca gcagattgcc
     3481 gactggtcac atcaattgaa aatcagtttg aatgctagca cgatcacgcc taaagatatc
     3541 gatgaattga aagaagcaga agctgatttc tcacagattg aagcttggca taattattat
     3601 ccacgtcccg aaacagggct ggacaaagaa tggtatcaga aaaaaaatca atggctcaaa
     3661 attcaaggtc tccttgtcca aggttttgta ccaggagata cagagttgag aggcccacta
     3721 tatcaaggat tgccaacttt ggaagaacat cgagggatcc atcctcttgc aggcgctcta
     3781 gatctattag caagtaacac ggatattgtt tacatcggag atgcaggtct gtcagaaaac
     3841 gttcaggagc aatttgcttc tttccaaaaa gaacagacag tcttgctcca tacggaacct
     3901 gtagatgaag aattttacga atatatatta gggaagcata cgaatcggca agacgatgca
     3961 agagatgtca tacgaagcgt agatgcacgt tttcgagaga ttccgcctat cccagcaaga
     4021 aatacagcta cgcgtatgaa agggtctatt actttagata acgaaaagta tttgcgctat
     4081 atgggtgaga tccaattaac caaatatgat ttgccagcgg atgaaaaagt gaatgtagta
     4141 gctaaagtta tcaaagaaga gctaccattg atcaaccaaa tcaaagctgg tatgaactat
     4201 caatttataa gaaaagaagg aagataaaaa tgagcaaaat caatttagag aacctaacaa
     4261 cggaacgcag aaaccaagaa acttttggat tagatgagat gagtatttct caagccgttc
     4321 aactaatgaa caaagaagat cagaaagtgg ccgctgcggt tgaaaaagaa ctgacaagta
     4381 tcgaaccagt gattgctaac acgattgagt cctttaaaaa aggtggacgt ctgatttata
     4441 tgggagccgg aacaagtgga agactaggtg tgttagatgc tgctgaatgc gtaccgacat
     4501 tcggtgtaga accggaaatg gtcataggtc taattgctgg tggagaagaa gcaatgactg
     4561 tagctgtcga aggggccgaa gattcttatg agctagggca agaagatcta atcaagctga
     4621 atttgacaaa aaatgatata gttattggta tcgcagctag cggtcgtaca ccatatgtga
     4681 tcggtggatt agattacgca aaatcgatcg gtgctgtcac aggaacgatt tcttgcaata
     4741 aacaagccga aatcagtaaa cacgcggatt tcccaatcga agtcgatgca ggtccagaat
     4801 ttttgacagg ctctactcga ttgaagtcag gaacagcaca aaaacttatc ttgaatatga
     4861 tttctactat cagtatgatt ggtataggta aagtattcaa taacttgatg gtcgatgtca
     4921 aaccaacaaa tgaaaaacta atcgaacgct caaaacgaat catcatgcaa gcgacagaag
     4981 ctgattacga aacagctgaa aagtactttg aagaagcaga tcaaaatgtt aagctggcta
     5041 tcgtcatgat tttgacaaat agtgataaag aggaagctca agcaaaatta gctgaagcaa
     5101 acggatttgt aaaagggacc ttatgagggg gaaaagacca tggcaaaaaa cgaactgact
     5161 ttggaagaac tggcaaaaca aatttataaa ggggcaggcg gcatgggcaa tgtagaaagc
     5221 attgtccact gcatgactcg tgtgcgtatg aagatcatca atgatgatct tgtaaatgtc
     5281 tctgaattaa aagcgattcc aggagtgctt ggtgtagtag aagatgaaca gcttcaagtc
     5341 atcatcggac ctgggaaagt gaacaaagta gcacagcaaa tggttgattt ggcaggcgtg
     5401 aaattaggag aaaaactttc ttctgtaagt acagctacag caaattcatc agcatctgga
     5461 aaagaccgtg taaacgaacg ggcacaagaa atgaaagcgg cacaaaaatc gaaacaaaaa
     5521 ccatctaaaa tcaaatcggt attaaaagac atttctaata tctttgtgcc aatgattcct
     5581 gcatttgtcg gtactgggat cgtggcaggg attgccgcag ttttagcaaa cttagtcaca
     5641 gccggaacac ttgatgcagc aacatggcaa caatatatcg atgtgatgaa catcttgaaa
     5701 aacgggatgt ttagctacct agttatctat acaggtatca atgctgcaca agtttttggt
     5761 gcaacaccaa ctcttggtgg agttatcggg gccgttgtga tgctgacagg gatgaatccg
     5821 gaagcaccac tgaaaaactt atttactggt gaagctcttg cagctggaca aggtgggatc
     5881 ctaggtgtca tcttcgctgt ttggctattg tcaatcgttg agaaaaaact gcataaagta
     5941 gtacctgatg cggtagatat catcgttaca ccaactcttt cattgattgc catcggtctg
     6001 attgaaattt tcttcatcat gcctttagca ggtttcattt ctgatggcat ggtcggtggg
     6061 atcaactggg tacttggcgt tggtggagca tttgccggct tcgttctagg cacattattc
     6121 ttgccgatgg ttatgttcgg actgcatcaa attttgactc caatccatgt gcaaatgatt
     6181 gatgaaactg gtcgtacgct attgcttcca attcttgcga tggctggtgc aggacaagtg
     6241 ggtgcagcat tggcattatg gattcgctgc aaaaaagata aagaattaac agaaatgatc
     6301 aaaggagctt taccagtcgg gatcctagga atcggcgaac cattgattta cggagtaaca
     6361 ttacctctag gacgcccatt tattactgct tgtatcggtg gaggtatcgg cggggcagtg
     6421 atcggaatga tcggaaatgt cggggctatc gctattggac cttcaggagc cgctttgatc
     6481 ccattgatct ctgacggtaa atggttaggc tacatccttg gattattggc agcttatgct
     6541 ggtggcttcg ttgctacatt cttcttcggt attcctaaag accgtttaga aaaagaagaa
     6601 ttagcgactg aaacagccac agtaaatgaa gtgcctgaaa aagtagtacc tcaaacttca
     6661 gctgaaagtg tgactatcct ctcatcagtt gctgacggaa cagttgtttc tttagataaa
     6721 gcaagtgatc cagtttttgc acaaaagatg atgggtgaag gctactttgt agaaccagaa
     6781 aacggacata tctattctcc agttactggg gtagtcagct ctgtcttccc aacgaaacac
     6841 gctattggga tcacaacgcc aaatggttta gaaatcttgc ttcatatggg aatcaacaca
     6901 gttgacttag gcggtacgcc atttgatgtg aaagtaacag aagggcagca agtgacacct
     6961 gatacgcttg tagcagaagc tgacttagaa gcgatcaaag cagcaggcaa agaaacatcc
     7021 atgatcgtct tggtaaccaa tatggatcgt gtaaaaaact ttgtcttgga aaaaacaggt
     7081 aaagcaaaag caaaagcacc tgtgatggat gtcaaagctt aatatgatag aaacaataga
     7141 aacgaaagaa gaaactgaaa tctatttttc agcttcttct tttttattat ttattgtttt
     7201 ctgctaaaat ctgataaatt cgtgtcacat cagatggtgt tccccgtaat tcataatctg
     7261 ccaagaaagg aaagccaaat gtttgtgcat attgtttggc tgttagacaa tactgattat
     7321 tgaagttttt atttccgcta ccgactacac caagacaaag tttatgattg tcttcatgtt
     7381 ccagatattc acgcatcgtt tcggtcaaga tttctgtatc accgttatct aaaccatttc
     7441 cgccgtctaa ataagtagga acgaaagtaa aaaatggttc tgtttctttc gtaaaatcac
     7501 tattttcatg gatttctttt aaagtaactt caggattgac aggatcttct gcatgcattt
     7561 tttcagcata ttctgctaga tgtttggcaa aggcacgcgt atttcctgaa atcgatatat
     7621 ataaaatgtt catggatcat cctccaattc tttcctttta gtatagcatc ttttttaagt
     7681 tttttggtgg tgtaatttgg aaagggacta ggcgctttgc ttgtataatg agaaaaaagc
     7741 cggtagaatt atatttctat taaagttttt attaattaag taaaaataaa aaaggtttgt
     7801 tatacttttt gataaaagcg gttttatttt ggaggatgct tacatggata cagagcaaca
     7861 gaaaaaagga attggtttaa ttccacttgc tgccttagta attggttcag cgattggagg
     7921 tggcgtattt ggtattatga cggacatctc cggatcggct ggcggcccag ctattttcag
     7981 ttgggtactc gtaggaacag caatgttgat gctctccttg tcgatcaata atatcaataa
     8041 aaaacgtcca gatttaaaag gcgggatctt tagttacgca gaagaaggtt ttggccgttt
     8101 cgctggtttt atcagcggtt ggggttactg gctcacatgc tggttaggca acgtagcttt
     8161 tgcaacattg ttgatgagcg cattaggata tttcttccca gtatttgaag gtgggcaaaa
     8221 tctggcatcg atcgtgatga gtacagtctt tttatgggga tatgcatggc tagtgaatcg
     8281 gggagtcgaa aatgctagtt tcgtcaatgc tatcgtcaca ttctgtaaat tagtgccatt
     8341 atttttattt attgtagtag ctatcagcac gttttcgatc caacaattca ttgataactt
     8401 ttctagcgac attatattga atgcgaatgg acagccgatc ccgttttcca gtcaagtatt
     8461 gagttgcatc atggtgatgc tttgggtatt tgttggtatt gaaggagcct ctgtcgtagg
     8521 ttcacgagca aaaaaacaat cagatgttgg gaaagcaacg attctaggga tattcggctt
     8581 gattattatt tacgtcttgg tttccatgct tccatatggg acaatgacga tgaatcagct
     8641 gcaaacgatc aaggcgcagc ctgctatggg ctatgtgttt gaaatgatgg ttggtaaatg
     8701 gggagcagtc gtgatcaacg gcggtttgat catttcttta gttggtgttt ggctctcatg
     8761 gacgatctta ccgattgaaa caatgggaaa catggctgaa gacggacttt tgcctaaagc
     8821 atggggaaaa ctcaacaaaa atggggcacc aacttatgct atcgtattga cgacgttatg
     8881 tacgaatgtc tttttacttt cattattatt cactgacagt gcttataact ttgcttacac
     8941 acttggtaca gcagctattt tcttcacgtg gcttttctta ggattgtacc aaatgaaact
     9001 ttcttatcag cggaaagaat gggtccaatt tttagtaggg cttttagcaa gtgcctttca
     9061 agtatgggct atgctagtcg tagcctttaa tgaagtaatg cttgttcttg ttcttttctt
     9121 gccaggcatt tatttttacc tcaaagcacg taaggagcaa aacgagcctg tacaacatat
     9181 ccacttagat aagattttac ttataggtgt gacgatggtc ggagtgattg caatggtttt
     9241 atttgccact gggatgcttc acgtatagaa gtataaaaga agtataaaga gaggttgtga
     9301 gcctgtcgtt tattcaaaaa catactagga aaggagctgt gacacaaatg ttgtcacagc
     9361 tcctttttca gaataaacgg tgtttaagag acagccaatc ggcaataagg ctgaacgtct
     9421 ctttcacagc ctcttttttg atgagtcatc atacattttt tcgcttaaat acgagatatc
     9481 ctaatgctaa gaacaaaacg atgtaagcta aattaccgaa tagcatttga ttagtagaaa
     9541 gatgagtcaa ttgcttcatg atctcttcgt ttccaatttg gttttgcaga ttcaacatac
     9601 tgatcggatt ccatttgatc cactcccatt tttggatgag tgcaataagg ataccagata
     9661 tgattgacga agcaaaataa aagacgattc ctgctgagat ggatgcgccg gaactattga
     9721 taaagcaagc aatcatcaac actaaactta aaatcagcca taaaccgata taactaccca
     9781 aagtataagt gaacaagctt tggatcagtg tttgaccagt agaaaccttc tccgtgaagg
     9841 aaatcgaagg gaaaagaatc agtttcatca acacagtgac aataatagct aaaaggtata
     9901 aataaacaga ataaataact aatgtgatcc atttgcttac taatatttct cctcgagaat
     9961 actttcgata aaggagattt ttcaatgtac catattgtgc ttccatcgaa atgatcgtgc
    10021 ttgcagctgc aatcatgata aagacaatcc aagatgtggc actaaaaagc tgagcagtca
    10081 tttcaactgg atcaatgatc gtcgtgtatt ttttggctag aagtgctgtt cctatgagca
    10141 aaacaactaa tagaacagat aaaacagcag ttgacttttt cttgatcaat ttaaataatt
    10201 cttgttttac aagcaatgtc atttttcttc ttcccccttt actgaatcca gaatttccaa
    10261 tagagaagat tccaaatcat tcgtcagatg ctggacgtct gtcacatgga tattttgtgc
    10321 agacaatagc tggatcacgg ttcctaataa ttcttcgttt tcttttaaaa gcttgatttt
    10381 atcaggatct tccaaagtat atccagcttc tgtcaagatc gttcgagcag ctagatcatt
    10441 tgttgtagag ataacgatga aatcttgcgc tgtttggttc aattcagcca ttgtacattg
    10501 tttgatgatc tttccttgat tgatcaggat cagctcatcg accatttttt caagctcgct
    10561 gagtaagtga ctggagatca agaaagaggt gccctgagta gccaagtcgg taatgatttt
    10621 tcgcacatct cttgttgctt gtggatccaa accgttcatc ggctcatcta aaataacgat
    10681 agacggatgg ttcagtaagg caagagcaat tcccaatttt tgtttcattc ccaacgagta
    10741 ggatttcgct tttttgtgaa tgtacttttc catcttgagc tgattcacga tagtgtcgat
    10801 agcagataca tcgttggttt cagagaataa cttcagatga tcatagcctg ttaagaatgg
    10861 atagataccc gggtattcga tcaatgctcc tacttgttcc aaccctttat gactagaggg
    10921 agaaatctca ttttcaccaa tacagattgt tccatctgag taatggatca gtcctaaaat
    10981 agctttcatg atcgttgttt ttccagcgcc attaggacct actaatccaa cgattttgcc
    11041 aggggataag ctaaaactga catcgctgag ggcttgtttg tgaccaaagt tcttggataa
    11101 ttcttggacc gaaagacttt tcaaaaatat tcactccttt tctatagata gtacaagcat
    11161 acacctgatg agtggagaag tcgctaggtc attggactta tttcttattt tttcataaaa
    11221 aaattgaata ttaaataaaa aggcttattt tttctgatca gtacttattc caagtgtcta
    11281 gtggccgcta gtaatcttga tgccaatgat actgatgagt agcatcccca caaagaacca
    11341 agtggaaaga ttcagcttat catgaaacaa gaccacacca attatgaccg aaccaactgc
    11401 gccaatccct gtccagatag gataagagag actcatcggc aaacttttga ttgcctttga
    11461 caaaaagtaa aaactagcaa tcatgccaat cactgtgtaa aaagaataat tgattttaga
    11521 aaaaccatca ctccatttca tcatcgttga ccagaatact tccaaaattc ctgctatgat
    11581 taattccatc catgccatac ttacattcct ccttaactat aaaaaa
//
LOCUS       DACCCS010000287        11422 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.guided.082, whole genome shotgun sequence.
ACCESSION   DACCCS010000287 DACCCS010000000
VERSION     DACCCS010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.guided.082"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            39..977
                     /locus_tag="IVK82_003113"
                     /pseudo
     CDS             39..977
                     /locus_tag="IVK82_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            980..3163
                     /locus_tag="IVK82_003114"
     CDS             980..3163
                     /locus_tag="IVK82_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP6394088.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3160..4161
                     /locus_tag="IVK82_003115"
     CDS             3160..4161
                     /locus_tag="IVK82_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP6394089.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4176..5090
                     /locus_tag="IVK82_003116"
     CDS             4176..5090
                     /locus_tag="IVK82_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6394090.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5335..5451
                     /locus_tag="IVK82_003117"
     CDS             5335..5451
                     /locus_tag="IVK82_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP6394091.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5467..7386
                     /gene="tet(M)"
                     /locus_tag="IVK82_003118"
     CDS             5467..7386
                     /gene="tet(M)"
                     /locus_tag="IVK82_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP6394092.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7484..8857
                     /gene="tet(L)"
                     /locus_tag="IVK82_003119"
     CDS             7484..8857
                     /gene="tet(L)"
                     /locus_tag="IVK82_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP6394093.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9421..10683
                     /locus_tag="IVK82_003120"
     CDS             9421..10683
                     /locus_tag="IVK82_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAP6394094.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10928..11410
                     /locus_tag="IVK82_003121"
     CDS             10928..11410
                     /locus_tag="IVK82_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP6394095.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNGSVAKF
                     "
BASE COUNT         3771 a         1907 c         2388 g         3356 t
ORIGIN      
        1 acttggagat aacatcaaaa agttacctat cgctataacg tggcagatgg aaagaaacct
       61 tgccagagat ttcccatgaa atcaatatcg tcaatctgac ttctgatgag aaaaacaaag
      121 gcttacttga cccttatgta attatgaaaa ataccaaaga ttctgaatca ctggctattg
      181 atattttgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc ccaatcctta
      241 gaaaagccat tcgtgcagta accaatagtg aagtgcgagg gttgatgaaa gtgattgaag
      301 aattacgggt tgagaatacg ccactaagta ccagcatagc cgaccatatc gaaagtttta
      361 cagactatga ctttgcccat ctgcttttta gtgatggtta tgtagagcag tctatcagcc
      421 ttgaaaaaca actgaacatt atacaggttg ccgacttggt acttcctgac aaggaaactt
      481 cctttgagga atataccaca atggagttac tatcggtagc aatgctgatt gtcattagta
      541 cctttgcgtt ggactttatc catacagacc gaagtatttt caagattgta gacttagacg
      601 aagcatggag cttcttacag gtggcacaag ggaaaacact atctatgaag ctggttcgtg
      661 ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagat gacctcttag
      721 atgaaaaact gaaaaataac ctcggcttaa aatttgcctt tcgttccact gaccttaacg
      781 agattaaaaa aacccttgcc ttttttggtg tagacccaga ggacgaaaac aatcagaagc
      841 gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt cgtgtcggtg
      901 tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc agaccacctg
      961 tgcgaaaaga ggtgtaaatg tgaaaccatc aatattaaac agaataaaat caaactggac
     1021 gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga ttgccatttt
     1081 tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg atacggtcaa
     1141 tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt tttatgtgga
     1201 taatagctgg ggctggctac cgtggaactg gtcggacgga attggaaaac aagtcatgta
     1261 tggactatat gccattacca attttatttg gacaatcagt ctttatgttt ccaatgcgac
     1321 gggttactta gtacaggaag cctattcctt agacttcatt tccgctacag cagattccat
     1381 tggcaagaat atgcagaccc ttgcaggggt tacgcctagt ggactatcaa cagagggttt
     1441 ctatgttgga ttccttttac tcttgatttt ggttcttggg gtttatgttg cctatacagg
     1501 actgataaag agagaaacca caaaggcaat tcatgccctc atgaattttg tgctggtttt
     1561 tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa tcaatgactt
     1621 ttcatcagac atcagcaacg ctagtttgtc actgggtaca aaaattgtca tgccccattc
     1681 agatagtcaa ggcaaggaca gtgtggactt gatacgtgac agcctatttt ccatacaggt
     1741 tcagcaaccg tggctactgc ttcaatataa cagttcagac attgaaagta ttggtattga
     1801 ccgtgtggaa agcctgctct caaccagtcc agattccaac aatggggaag acagagaaaa
     1861 aattgttgcg gaagaaattg aagatagaag caataccaat atgacgatta caaagacgat
     1921 taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt ccatatttgt
     1981 attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct atgctatgtt
     2041 tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt caaaacgagc
     2101 cataacaaag ctctttaata ccattttgac acgagctgga atcacattga ttattacgac
     2161 agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt tctttttgat
     2221 tgcttttcta cagattgtga cctttgcagg aatctacttt aagctgggcg atttaatgag
     2281 tatgttttct ctacagagta acgattctca aagtgtggga agtcgtatga tgagaaaacc
     2341 tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac gctccgtgac
     2401 tgctttaggg gctgggtctg ccattgccag tgctacgggt aaaaaaggac agtcgggttc
     2461 gggaagttct gcgagtacac aagcagatca ctctcgacca aacgggcagg aaaaatcaac
     2521 actaggaaag cgtatcggtc aaaccatcgg tacagtagct gataccaaag acagaatggt
     2581 agatactgct ggtaatttga aagaacaggt taaggatttg ccgaccaatg caagatatgc
     2641 agtatatcaa ggaaaatcta aagtgacgga taatgtccat gatttaacca gaagtatttc
     2701 tcaaaccaaa gcggataaag ccagtggacg caagaaacag caggaacaaa gacggaaaac
     2761 cattgcggag cgtcgctctg aaatggaaca ggtcaaacag aaaaaacagc cagcttcttc
     2821 cgttcatgaa agaccagcca ctaagcaaga acaatctcat gatggacaga cttcaaggca
     2881 aacaactgta caggcttcct atagggaatc tcaacaagcc aaacaagagc gtccaacagt
     2941 taagtccgat tcttcaagtt taaaaacgga acgtcaaagt aatacagttc aagaaagaac
     3001 ggttcaaaag ccagtaactt caactgcacc aacagataga gcttcacaac gttcaatcac
     3061 aaaagaacgt cagtctactg ttcaaagagt accactacaa aatacaaaaa gtagaccacc
     3121 aatcaaaacc gccaccatta agaaaggcag taagaaacca tgaagctgaa aactttagtg
     3181 attggtggtt cgggattatt cttgatggtc ttctcactgc ttctgtttgt cgccatttta
     3241 ttttcagatg aacaagatgg cggtttttcc aacattcact atggcggtgt ggatgtttcc
     3301 gcagaagtgc tggctcataa gcctatggta gaaaaagttg ccaaagaata tggaattgaa
     3361 gaatacgtca atatacttct tgcgattata caggtggaat cgggtggtac agcagaagat
     3421 gttatgcagt cctcggaatc cctcggtctt ccacctaatt cattgagtac agaagaatcc
     3481 attaagcaag gtgtgaagta tttcagtgag ttattagcca gtagcgaaag gcttggtgta
     3541 gatttagaat cggttatcca gtcctacaat tatggtggtg gtttcttagg atatgtggct
     3601 aatcgtggga ataaatatac ctttgaactg gctcaaagtt tctcaaaaga gtattcgggt
     3661 gacgaaaaag tgtcttaccc caatcccata gccataccta tcaatggggg ctggcgatac
     3721 aattacggga atatgtttta tgtgcaactg gtaacgcagt atcttgtcac aacagagttt
     3781 gatgatgata cagtacaagc catcatggac gaagctctga aatttgaagg ctggaagtat
     3841 gtctatggcg gagcttcccc gactacctct tttgattgta gcggactgac acaatggacg
     3901 tatggaaaag ctggaatcaa cttaccacga acagcacaac agcaatatga tgtaacccgg
     3961 catatcccat tattggaagc aaaagccggc gatttggttt tctttcattc tacctataac
     4021 gctggctctt atattactca tatcgggatt tatttaggcg ataaccgtat gtttcatgca
     4081 ggcgacccta tcggttatgc cgacttaaca agctcttact ggcaacagca tttagtggga
     4141 gcaggacgaa tcaaacaata agaaaggacg attcaatgat gaaattcgga aaaaatcaga
     4201 atacagaaaa acagacacca aaggaaaaga aacctcgtgt ctataaggtc aatcctcgta
     4261 aaaaggttgt gattgtccta tgggtacttt taggacttag tttcagcttt gcgatattca
     4321 agcactttac agccattgat acccatacca ttcatgaaac caccatcata gaaaaggaat
     4381 atgtcgatac tcatcatgtg gaaaattttg tagagaactt tgcgaaagtc tactattcat
     4441 gggaactaaa cgacaagtcc attgataatc gcatagaaaa cctgaaagca tatctgacag
     4501 aggaacttca agctctgaat gtcgatacag ttcgtaaaga tattcctgta tcgtcttctg
     4561 taaaaggatt tcagatatgg acggtagaag cagatggcga caatcaattt gatgtaacct
     4621 acagtgtaga ccaactcatt acagaggggg aaaatacaaa gaccgtccac tctgcttata
     4681 tagtgagtgt ctatgtagac agtactggaa atatggtcct cattaagaat ccgaccatta
     4741 ctagcatacc aaagaaatca gactataaac caaaagctat tgaaagtgag ggtacggttg
     4801 attccattac aaccaatgaa atcaatgagt ttttaacgac gttcttcaag ctctatccta
     4861 cagcgacagc cagtgaactt tcctactatg tgaatgacgg gatattaaaa ccaatcggaa
     4921 aagagtacat ctttcaagaa ctggtaaatc ctatttacaa tcgtaaggat aatcaagtca
     4981 cggtatcgct gacagtggag tatatcgacc agcagaccaa agcaacgcag gtatctcaat
     5041 ttgatttggt acttgaaaag aacgggagta attggaagat tatagaataa caaatattgg
     5101 tacattatta cagctatttt gtaatcacgt actctctttg ataaaaaatt ggagattcct
     5161 ttacaaatat gctcttacgt gctattattt aagtatctat ttaaaaggag ttaataaata
     5221 tgcggcaagg tattcttaaa taaactgtca atttgatagt gggaacaaat aattggatgt
     5281 ccttttttag gagggcttag ttttttgcac ccagtttaag aataccttta tcatgtgatt
     5341 ctaaagtatc cggagaatat ctgtatgctt tgtatgccta tggttatgca taaaaatccc
     5401 agtgataaga gtatttatca ctgggatttt tatgcccttt tgggcttttg aatggaggaa
     5461 aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcggg aaaaactacc
     5521 ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag cgtggacaaa
     5581 ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac aattcagaca
     5641 ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac gccaggacat
     5701 atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc aattctactg
     5761 atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc acttaggaaa
     5821 atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat tgatttatca
     5881 acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa acagaaggta
     5941 gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg ggatacggta
     6001 atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt agaagcattg
     6061 gaactcgaac aagaggaaag cataagattt cagaattgct ccttgtaccc tgtttatcat
     6121 ggaagcgcaa aaagcaacat agggattgag cagcttatag aagtgataac gaataaattt
     6181 tattcatcaa catacagaaa gaagtctgaa ctttgcggaa atgtcttcaa aattgaatat
     6241 tcggaagaaa gacaacgtct tgcatatgta cgcctttatg gcggaatcct gcatttgcgg
     6301 gattcggtta gaatatcgga aaaggaaaaa ataaaaatta cagaaatgta tacttcaata
     6361 aatggtgaat tatgtaaaat tgataaggct tattccgggg aaattgttat tttgcaaaat
     6421 gagtttttga agctaaatag tgttcttgga gatacaaagc tattgccaca gagagagaga
     6481 attgaaaatc cgctccctct gctgcaaaca actgttgaac cgagcaaacc tcaacaaagg
     6541 gaaatgttac ttgatgcact tttagaaatc tccgacagtg acccgcttct acaatattat
     6601 gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg
     6661 attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc
     6721 atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca
     6781 aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg
     6841 cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg
     6901 gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa
     6961 atctgtttta agtatggctt atactatagc cctgttagta ccccagcaga ttttcggatg
     7021 cttgctccta ttgtattgga acaagtctta aaaaaagctg gaacagaatt gttagagcca
     7081 tatcttagtt ttaaaattta tgcgccacag gaatatcttt cacgagcata caacgatgct
     7141 cctaaatatt gtgcgaacat cgtagacact caattgaaaa ataatgaggt cattcttagt
     7201 ggagaaatcc ctgctcggtg tattcaagaa tatcgtagtg atttaacttt ctttacaaat
     7261 ggacgtagtg tttgtttaac agagttaaaa gggtaccatg ttactaccgg tgaacctgtt
     7321 tgccagcccc gtcgtccaaa tagtcggata gataaagtac gatatatgtt caataaaata
     7381 acttagtgta ttttatgttg ttatataaat atggtttctt gttaaataag atgaaatatt
     7441 ttttaataaa gatttgaatt aaagtgtaaa ggaggaatta attatgaagt gtaatgaatg
     7501 taacagggtt aaatacaacc aaattttaat ttggctttgc gttttatctt tttttagcgt
     7561 attaaatgaa atggttttga acgtctcatt acctgatatt gcaaatgatt ttaataaacc
     7621 acctgcgagt acaaactggg tgaacacagc ctttatgtta accttttcca ttggaacagc
     7681 tgtatatgga aagctatctg atcaattagg catcaaaagg ttactcctat ttggaattat
     7741 aataaattgt ttcgggtcgg taattgggtt tgttggccat tctttctttt ccttacttat
     7801 tatggctcgt tttattcaag gggctggtgc agctgcattt ccagcactcg taatggttgt
     7861 agttgcgcgc tatattccaa aggaaaatag gggtaaagca tttggtctta ttggatcgat
     7921 agtagccatg ggagaaggag tcggtccagc gattggtgga atgatagccc attatattca
     7981 ttggtcctat cttctactca ttcctatgat aacaattatc actgttccgt ttcttatgaa
     8041 attattaaag aaagaagtaa ggataaaagg tcattttgat atcaaaggaa ttatactaat
     8101 gtctgtaggc attgtatttt ttatgttgtt tacaacatca tatagcattt cttttcttat
     8161 cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc aggaaagtaa cagatccttt
     8221 tgttgatccc ggattaggga aaaatatacc ttttatgatt ggagttcttt gtgggggaat
     8281 tatatttgga acagtagcag ggtttgtctc tatggttcct tatatgatga aagatgttca
     8341 ccagctaagt actgccgaaa tcggaagtgt aattattttc cctggaacaa tgagtgtcat
     8401 tattttcggc tacattggtg ggatacttgt tgatagaaga ggtcctttat acgtgttaaa
     8461 catcggagtt acatttcttt ctgttagctt tttaactgct tcctttcttt tagaaacaac
     8521 atcatggttc atgacaatta taatcgtatt tgttttaggt gggctttcgt tcaccaaaac
     8581 agttatatca acaattgttt caagtagctt gaaacagcag gaagctggtg ctggaatgag
     8641 tttgcttaac tttaccagct ttttatcaga gggaacaggt attgcaattg taggtggttt
     8701 attatccata cccttacttg atcaaaggtt gttacctatg gaagttgatc agtcaactta
     8761 tctgtatagt aatttgttat tacttttttc aggaatcatt gtcattagtt ggctggttac
     8821 cttgaatgta tataaacatt ctcaaaggga tttctaaatc gttaagggat caactttggg
     8881 agagagttca aaattgatcc tttttttata acaggaattc aaatcttttt gttccattaa
     8941 agggcgcgat tgctgaataa aagatacgag agacctctct tgtatctttt ttattttgag
     9001 tggttttgtc cgttacacta gaaaaccgaa agacaataaa aattttattc ttgctgagtc
     9061 tggctttcgg taagctagac aaaacggaca aaataaaaat tggcaagggt ttaaaggtgg
     9121 agattttttg agtgatcttc tcaaaaaata ctacctgtcc cttgctgatt tttaaacgag
     9181 cacgagagca aaacccccct ttgctgaggt ggcagagggc aggttttttt gtttcttttt
     9241 tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt tggtcggcac tgccgacagc
     9301 ctcgcagagc acacacttta tgaatataaa gtatagtgtg ttatacttta cttggaagtg
     9361 gttgccggaa agagcgaaaa tgcctcacat ttgtgccacc taaaaaggag cgatttacat
     9421 atgagttatg cagtttgtag aatgcaaaaa gtgaaatcag ctggactaaa aggcatgcaa
     9481 tttcataatc aaagagagcg aaaaagtaga acgaatgatg atattgacca tgagcgaaca
     9541 cgtgaaaatt atgatttgaa aaatgataaa aatattgatt acaacgaacg tgtcaaagaa
     9601 attattgaat cacaaaaaac aggtacaaga aaaacgagga aagatgctgt tcttgtaaat
     9661 gagttgctag taacatctga ccgagatttt tttgagcaac tggatccagg agaacaaaaa
     9721 cgattttttg aggaaagtta taaattattt tccgaacgat atggcaagca aaatattgct
     9781 tatgcaacag ttcataatga tgagcaaacc cctcacatgc atttaggtgt tgtgcctatg
     9841 cgtgatggaa aactgcaagg aaaaaatgtg tttaatcgtc aagaactgtt atggctacaa
     9901 gataaattcc ccgagcatat gaaaaaacag ggttttgagt tgaagcgtgg tgaacgtggc
     9961 tctgaccgta aacatattga gacagctaaa tttaaaaaac aaactttgga aaaagagatt
    10021 gattttctag aaaaaaattt agcagttaaa aaagatgaat ggactgctta tagcgataaa
    10081 gttaaatcag atttagaagt accagcgaaa cgacacatga aaagtgttga agtgccaacg
    10141 ggtgaaaagt ccatgtttgg tttgggaaaa gaaataatga aaacagaaaa gaaaccaacc
    10201 aaaaatgttg ttatatcgga gcgtgattat aaaaacttag tgactgctgc gagagataac
    10261 gataggttaa aacagcatgt tagaaatctc atgagtactg atatggcgag agaatataaa
    10321 aaattaagta aagaacatgg gcaagttaaa gaaaaatata gtggtcttgt agagcgattt
    10381 aatgaaaatg taaatgatta taatgagttg cttgaagaaa acaagtcttt aaagtctaaa
    10441 ataagcgatt taaagcgtga tgtgagttta atctatgaaa gcactaagga attccttaag
    10501 gaacgtacag acggcttaaa agcctttaaa aacgttttta aggggtttgt agacaaggta
    10561 aaggataaaa cagcacaatt ccaagaaaaa cacgatttag aacctaaaaa gaacgaattt
    10621 gaactaactc ataaccgaga ggtaaaaaaa gaacgaagtc gagatcaggg aatgagttta
    10681 taaaataaaa aaagcacctg aaaaggtgtc tttttttgat ggttttgaac ttgttctttc
    10741 ttatcttgat atatatagaa ataacgtcat ttttatttta gttgctgaaa ggtgcgttga
    10801 agtgttggta tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt gcacagaaaa
    10861 accccatctg ttaaagttat aagtgaccaa acaaataact aaatagatgg gggtttcttt
    10921 taatattatg tgtcctaata gtagcattta ttcagatgaa aaatcaaggg ttttagtgga
    10981 caagacaaaa agtggaaaag tgagaccatg gagagaaaag aaaatcgcta atgttgatta
    11041 ctttgaactt ctgcatattc ttgaatttaa aaaggctgaa agagtaaaag attgtgctga
    11101 aatattagag tataaacaaa atcgtgaaac aggcgaaaga aagttgtatc gagtgtggtt
    11161 ttgtaaatcc aggctttgtc caatgtgcaa ctggaggaga gcaatgaaac atggcattca
    11221 gtcacaaaag gttgttgctg aagttattaa acaaaagcca acagttcgtt ggttgtttct
    11281 cacattaaca gttaaaaatg tttatgatgg cgaagaatta aataagagtt tgtcagatat
    11341 ggctcaagga tttcgccgaa tgatgcaata taaaaaaatt aataaaaatg gttctgttgc
    11401 aaagttttaa ataaagaata aa
//
LOCUS       DACCCS010000288         8619 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.guided.096, whole genome shotgun sequence.
ACCESSION   DACCCS010000288 DACCCS010000000
VERSION     DACCCS010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.guided.096"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(565..792)
                     /locus_tag="IVK82_003122"
     CDS             complement(565..792)
                     /locus_tag="IVK82_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6394096.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            complement(801..1274)
                     /locus_tag="IVK82_003123"
     CDS             complement(801..1274)
                     /locus_tag="IVK82_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP6394097.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            2504..3070
                     /gene="amaP"
                     /locus_tag="IVK82_003124"
     CDS             2504..3070
                     /gene="amaP"
                     /locus_tag="IVK82_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP6394098.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            3083..3271
                     /locus_tag="IVK82_003125"
     CDS             3083..3271
                     /locus_tag="IVK82_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP6394099.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            3284..3841
                     /locus_tag="IVK82_003126"
     CDS             3284..3841
                     /locus_tag="IVK82_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6394100.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            3873..4115
                     /locus_tag="IVK82_003127"
     CDS             3873..4115
                     /locus_tag="IVK82_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6394101.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            4456..5670
                     /locus_tag="IVK82_003128"
     CDS             4456..5670
                     /locus_tag="IVK82_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP6394102.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            5701..5919
                     /locus_tag="IVK82_003129"
                     /pseudo
     CDS             5701..5919
                     /locus_tag="IVK82_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6338..6811
                     /gene="dfrF"
                     /locus_tag="IVK82_003130"
     CDS             6338..6811
                     /gene="dfrF"
                     /locus_tag="IVK82_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP6394103.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            6938..7594
                     /locus_tag="IVK82_003131"
     CDS             6938..7594
                     /locus_tag="IVK82_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP6394104.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            7776..8306
                     /locus_tag="IVK82_003132"
     CDS             7776..8306
                     /locus_tag="IVK82_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6394105.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         3020 a         1282 c         1694 g         2623 t
ORIGIN      
        1 ctcgagttac gagtttggaa cagcttttgt ctacagtctg tcattattat caaaataatg
       61 ttaaaaataa aaaagaattg tccatcactg ttattcaaat cattttatca aaactacata
      121 gtaaatgtta aaaagcaaca tgacataata tatagaaggc tttacagtat atatgggagc
      181 ttactaaaaa agaatggagt aagagttaac acaatgggcc attagctgaa gttttaagca
      241 cttcaaggta tgaaacagaa tggcctgtaa tactacatga ttatttagac tgctcaaact
      301 gctatcagtc tggtggaatg aataaagtat tatactaaaa gcagatccaa ctacaacgta
      361 acaggaaacc agttgtatca ggaaaagtga tagtacatgg agaagaaaag gccccctttg
      421 tatgagtcag tacatatctt atttatagat aaaacggtat tcaatcgaaa taaaaaatct
      481 gaataataga cgtaaataac ttctaaaagt ctcacacatc tatcattcag attatttttt
      541 atttactgtt tgttctactt tattttattc ttctggtata gtattcgagt catttttata
      601 ggtcaacaaa tcacctggtt gacactccaa aacttggcag attttttcga gagtggaaaa
      661 acggatggct tttgcttttc cattttttag aatagagatg tttgccatcg taatgcccac
      721 tttctcactc aattcagtca cactcatttt tttttgagct aatactacat ctaaattaac
      781 gataattgcc atgtcgcacc tcacactgtc aaatcatgat ctgtcttaag taaaatcgca
      841 tgttcaagca gtttttccag cacgcttgtt aatgtgtata ctgcaaaagg aatgaatacg
      901 acagcgattc ctaccaacat caaacctgga gcatcgtcta aatccgctac tttcataaat
      961 ttaggaagta agccaaaaaa ggcgaaacca ataaacaaaa tggttcgttc gatttttttt
     1021 agtatgttta agctttgagt ggaaaaagct tggttatctt cgattttttt caatagtttc
     1081 aataagaaat aagcaataac gatccctagg agaagggcaa agtaaatgac agaaacaaat
     1141 aatgtggtct gccaatcaaa attgaatcct ttttcgcttg tagcgatctg agtataaaaa
     1201 agtacgccga acaaaataaa tacggcactt atacctaaaa cgacaaattt taatagcaat
     1261 gttttaaatt tcatttttat ccctctttta tctttgatag ctaaaatata gcagaataat
     1321 tattgtaaaa caactatttt ttatcgaaaa acaataaaaa tatatcgttt ataaaaaatg
     1381 atagagtatg aaggtgtgga gtgcatttaa cagctttttt tgattgtaag aaacatttaa
     1441 aaccagttat tttagattct atgtgtgtct cttctataat gctagtttag atgcctggga
     1501 ttatattgga caatacagtt ggaggatttt ttattatcta gcgaagagaa gtgtaagtta
     1561 acagaactgt atgagaaaaa agacaactac gtagtagcta accgattcat taattttttc
     1621 aagttcatgt ttatttacgt atcagaattt ttcttattca atggtttggt gtaatcacca
     1681 cttatttgtc ctttattgct tatacaatgt tatacaaaag gaaaatcaag cattgacgca
     1741 tggggtggta ctatctagct gaaatagctt aataagcgca agtagatgca ttggattagt
     1801 ttataaatca aaagtgttag aggtattaag aagaagtaga aaaataaaat ggattgccga
     1861 ataaaaagta ccataaatcc taaaacatgt gccaacagat agatggcaca tgtttatttt
     1921 tttcgataat tttgtaacaa aaatacgaag atgaaaaaga atagagattc ccataatgta
     1981 gtagtccaac tgaattcttt actgatacca aataccatat ttcctagatt tgatacaatc
     2041 aaatagccaa tcgttatgat caatgaatag tacaatcttt tcatcatagg acatacctcg
     2101 tttttattta taagaatagt ataaaccaaa gaagtgaata agaaaaatga taatataaaa
     2161 ataaagtaag aacgcattaa agtaactaaa gttatttatt gtatttttta attctccgaa
     2221 aaaatttaat aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt
     2281 aatcttgaat aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa
     2341 gcaattgtta tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg
     2401 gtagcgcttt atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt
     2461 agagttattt acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa
     2521 aataatcggt gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat
     2581 caatgcgccg tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa
     2641 ttactacatg caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat
     2701 catcttgcta gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga
     2761 agatggcaaa ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca
     2821 cgaagaagag ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca
     2881 tatccatgtc aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt
     2941 gatgcaagat atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt
     3001 ggcagtcaca tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg
     3061 agtggaatag gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat
     3121 ctttgggggt cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac
     3181 attgttgatc ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc
     3241 aggaatagtt gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat
     3301 gaatacaaca aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg
     3361 tgaattgact tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt
     3421 agatggtcta ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa
     3481 cacagataac gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga
     3541 tctagatgtg atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga
     3601 attgatcagt actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa
     3661 tgttgcagac atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa
     3721 agtaacagaa gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa
     3781 atctgctgta ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata
     3841 agaagtaaga agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg
     3901 gcggtatcat tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca
     3961 tcgcaaacat cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt
     4021 ggggaccaac aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag
     4081 tattagtcgt ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg
     4141 aaatgaagaa caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt
     4201 tatatgaagt attctttatt ccttatgaaa aaatatcttt aaaaataaaa aaagacgaaa
     4261 ataaacggtt ttctataaaa agaagaccgt ttattttttg tctttatttg tgtgaaaaac
     4321 gaaaaagagc acaacacatc aagcttttat taattgtctg tcttttataa atgcaagtat
     4381 taattcaaaa aaacagaaga aaaaaacaac tacaccttct tttatactcg taatcgtaga
     4441 aaggaggcga aaaaaatgaa tacattacct atcaatacag gagatacagc attcatgatt
     4501 ttatgtacag caatggtttg cctgatgact cctggtctag ctttttttta cggcggatta
     4561 gcacggaaga ataatatact aacgatcatg tctcaatctt ttctctccgt tgggataaca
     4621 acgattatgt gggtatttgg tggattcgga ttggcctttg gacgggatat cggcggtgtt
     4681 attggaaacc cggcagattt ctttttgatg agacatgtta caagcttccc caacatgttt
     4741 catggtgcaa cgattccatt tttaatgttc tttctttacc aactgatgtt tatcgtgatt
     4801 accgttcctt tgatgacagg agcttttgca gggagattga atttaaaagg ctatattata
     4861 cttgttattt tttggaatct gctgatttat ttccctgttt gccactggat atggggtggc
     4921 ggatttttgg atcagatggg atttagagat ttcgctggtg gagcagtcat ccacacgaca
     4981 gctggattcg gctctttagc atgtatcctc acacttggtc aaagaaagat tcctttagga
     5041 gaaagaagtc agcatagcag tcttatggca gctgctatcg gtacaggact gctttggttt
     5101 ggctggttcg gttttaattc cggtggtgca cttcgagcag atgaacaagc agtcaatgca
     5161 tttggttcaa cattcatcgc tcttgcattt gcgatgatta cttggctgat cattgcaaaa
     5221 gtaaaaggaa atggctttga ttttgtcgac gtactaacag gatccgttgc tggattggca
     5281 gccattacgc cttgtgccgg ttatgtagaa gcaaaaagtg ccatgctgat tgggatcatt
     5341 gcgggtatcg tctgtcatgc tgctgttgat tttcgtaaaa agaaacaatg ggatgatgca
     5401 cttgatgtgt ggggtgttca tggcatgggc ggattcacag gtacgattct gatagggatc
     5461 tttgcatcta acagccattt actaacgaat ccaagcagct ggtacttctt agggattcaa
     5521 gtgattggtg tcatcattac tgccgtgtat gcctatgtgc tgacaacagt gattttaaaa
     5581 tcggcgatgc actttacaac gatcactact acaaaagaag aacaagaaga aggattggac
     5641 tttttcttct ttggcaacaa actcaattga aacaaattca aatagaaaag aggaagattc
     5701 atgcaacaaa gcaaaacgaa aattcctgca aaaaaattaa cgttctttgg ctttttggct
     5761 atgaccatct caatggtcgt ttcattatat gaatatccca cattcgcgac ctcaggcttc
     5821 agcttagtat ttttcttgtt attaggcggt ttgctatggt ttatccctgt ggcattgtgc
     5881 gcagcagaga tggcaacggt gaaaggatgg gaaaaaggtg tttcccaaac aaaaagatat
     5941 taagaaacag cccatgttta cctgcttgca aacaaaagaa tattgctact agactaagag
     6001 atactacata agtgtgttgg tatctctttt ttgttgccgt gaaacagcag actatttcat
     6061 ttctgcttga ttaccttaaa acttattagt ctatggtttg tcaagggctt gttgcgtgag
     6121 aaatgctgaa tatcaccctt tacaaaacat aggctgataa gtaacttttc aaaagcagaa
     6181 aagaaatggc aatgcttata acaatatgat ataatgcaga tgaagacaaa aaaggaattt
     6241 gtcgagctgg ttagttcgag ataattttga gtttgttaag atgtggtgca cttgatggaa
     6301 tcacaggtac gacatcatat tatggaggat gaaatagatg ataggtttga ttgttgcgag
     6361 gtcaaagaat aatgttatag gcaagaatgg taatatacca tggaaaataa agggagaaca
     6421 aaagcaattt agagagttaa caacgggtaa tgtggttatt atggggcgaa agtcttatga
     6481 agaaatcggt catccgttgc ctaatagaat gaatattgtt gtttccacca caacagagta
     6541 tcaaggagat aatttagttt cagttaaatc attagaagat gcattattat tggctaaagg
     6601 acgagatgta tacatatctg gtggatatgg actatttaag gaagctttgc aaatagtaga
     6661 taaaatgtat atcacagaag tagatttaaa tattgaagat ggagatacat tctttccaga
     6721 atttgatatc aatgattttg aagttttgat aggggaaaca cttggtgagg aagtgaaata
     6781 tacgagaaca ttttatgtaa ggaaaaaatg aattgagtaa attttggatt tagggtgttt
     6841 tcataaatat attgcttttt atgcatatat aaattgttgt aagacaaatt ccagtttgta
     6901 tggggcgata aaatcagaat ttgagagagg attgaatatg aataaggaat ggtctgaact
     6961 taataaaaca atgcaggcac aaataaaaaa gaaagatact tataagaggg gtattgatac
     7021 tttacttact ttacgaagtc agttaataca aaccttagta tctttcaaag aagaattatg
     7081 cagagaagat tttaactcaa tccccttcat aaacgctgat ggttatcata gtaagacgat
     7141 tgcttattct atttggcata ttttccgcat tgaagatata gttgtgcata cagtgataaa
     7201 tgaagatgaa caagtatttt ttgcaggcaa ttatcaagag cgtatcaatt cacctatcat
     7261 tacaacagga aatgaactca tgaaacagca gattgcagac ttttcaaaac agcttaatct
     7321 ggaagaatta tatttatata ttttcgaagt atgggaaagc actgagaaaa tgcttgaacg
     7381 gttatcttat gatgaattga aaagaaaaat accgaaagaa agaaaaggat acttagaatc
     7441 gttgaatgta gtaaacgaca atgaaaaagc aatttggctg attgattatt ggtgtaataa
     7501 agatatttgt ggactaattc aaatgccgtt ttcaagacat tggattatgc acacagaagc
     7561 ctgtttgcgt ataaagaaca agatacattc ataggcaaaa atttcagttt gtagaactaa
     7621 ggaaattgga atttaaggag gaataaagta tggttagtga ttttataaga gcagcatttc
     7681 catggatttt aatgggttta tttgtagcaa tcagttgttc ccttatgagt aaaaaagaga
     7741 aataaattcc tgtttgtatt tataaaagag aaaaaatgag aaaagtagtt ttatttattg
     7801 ccatgagcct tgacggatat attgcggacg gtaatggtgg agtagcttgg ctaaatggtc
     7861 atggaaatga caatgaaaac atcgacactt atacagaatt taccaaagat atagatactg
     7921 tcttaatggg gtggaatact tctcatcagg ttgttactga actttctccg caagaatggg
     7981 tatataataa atttacaact tatgtattaa cacataaaga gtgcaattct tcccaacaaa
     8041 ttcattttac aagtgaaaat cctgttttat tattggaacg gctaaaacaa gaagcaggaa
     8101 aggatatttg gatttgtggc ggagcaaatc ttgttcagca gttggtaagc aaaaatatga
     8161 ttgataaata ttacatttct gttattccta ctctgttagg aaatggggtt cgtcttttgg
     8221 gcaacataga cagggaaata aagttaaggc tttgtaaaac acagacttat aacggaataa
     8281 ccgatttgat ttatatgcgt agataactag agagaaacag aaaagctgtt aaaccgacgg
     8341 agcgatccga ccacgttggc gggaaaatcc ttaagatttt tccgccatgc ttgcaagggg
     8401 gcggttcgct tcggcggttc ggcgagcttg cgagccgttc aaagcccccg ttatctaaca
     8461 aggggggcac aaaaaacaag aaacactata cactaacaag cggaaaacgc tgtatttata
     8521 aggcaaaacc ccatttttac agtagtgata taaattgtac ctatatcact atgaaaattg
     8581 aatagtaaat atgagaaaga cagttgattt caaaaaata
//
LOCUS       DACCCS010000289         7909 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.guided.102, whole genome shotgun sequence.
ACCESSION   DACCCS010000289 DACCCS010000000
VERSION     DACCCS010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.guided.102"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            78..1514
                     /locus_tag="IVK82_003133"
     CDS             78..1514
                     /locus_tag="IVK82_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6394106.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1703..4150
                     /locus_tag="IVK82_003134"
     CDS             1703..4150
                     /locus_tag="IVK82_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP6394107.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4380..5447
                     /gene="rlmN"
                     /locus_tag="IVK82_003135"
     CDS             4380..5447
                     /gene="rlmN"
                     /locus_tag="IVK82_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP6394108.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5640..6743)
                     /locus_tag="IVK82_003136"
     CDS             complement(5640..6743)
                     /locus_tag="IVK82_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6394109.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7296..7844
                     /gene="aac(6')"
                     /locus_tag="IVK82_003137"
     CDS             7296..7844
                     /gene="aac(6')"
                     /locus_tag="IVK82_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP6394110.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2650 a         1345 c         1726 g         2188 t
ORIGIN      
        1 ttttattttg ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat
       61 aggaaaggaa gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt
      121 ggggctctgc ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag
      181 aatcagtctg ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg
      241 atttggcagt agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag
      301 gtctgaaagc ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag
      361 aagtcaatca agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg
      421 atattgaacc tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat
      481 ataatggttg ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat
      541 ttgaggcatt ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca
      601 cttcattagg ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt
      661 atgaagttaa ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt
      721 tagaaatgcc tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca
      781 gtaatcctga aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga
      841 tggatgtcta tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag
      901 gatgggcacc aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt
      961 ttcttgggat caattactat caaacggcga cgaatgcatt caatccatta aacggagttg
     1021 gtgctggaaa aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg
     1081 gaatgtataa aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg
     1141 acccagaagg attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac
     1201 tgattacgga aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg
     1261 acgactatcg aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg
     1321 atggagcaga agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga
     1381 acggttatca aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat
     1441 ctttggaacg atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg
     1501 gagagaacct ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct
     1561 tttcttgata aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag
     1621 aaagcgtcag ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac
     1681 ttgatgaaat gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa
     1741 gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg
     1801 gtttgccttg attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt
     1861 gattgcaaac ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt
     1921 agccgtatta ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg
     1981 acgctggttt ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt
     2041 atttggcaaa ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc
     2101 cagtgatata aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat
     2161 cctttatcat tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct
     2221 attactggca ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg
     2281 tatccaatca attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc
     2341 tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag
     2401 gcgcttagct gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc
     2461 ggtgagccag caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc
     2521 ggagcagcta agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct
     2581 agagaaacca gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga
     2641 gttgccagag gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca
     2701 agactatgaa ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga
     2761 tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg
     2821 tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca
     2881 gccggctgta ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc
     2941 tttagcggcg aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat
     3001 tgaagtacca aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc
     3061 aaaccaccca gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca
     3121 aacggctgat ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa
     3181 gtcagtagca attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt
     3241 aaaactgatg atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca
     3301 tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt
     3361 acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg
     3421 ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc
     3481 attcatcgta gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt
     3541 tgaagatgcg attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct
     3601 agctactcaa cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc
     3661 cagaatggca tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg
     3721 ggcagaaaag ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc
     3781 gatccgtgtg caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt
     3841 tacagatcag caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac
     3901 gacaggtgct ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt
     3961 tgaaatgcaa acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg
     4021 agcagcacgt ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag
     4081 caagcctcgg aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa
     4141 tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg
     4201 catgatgata ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc
     4261 ttgtcttttt agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat
     4321 taaggaaatg aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt
     4381 tggaaaaaca gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa
     4441 acggcgagaa aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa
     4501 gtaatttcac agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt
     4561 ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat
     4621 atttgtttga actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg
     4681 gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa
     4741 gcgggctgct caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga
     4801 tggtccaaca ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa
     4861 tggggatcgg tgagccattt gataattacg acaatgtaat ggactttttg catatcatca
     4921 atgatgccaa aggattggca attggtgctc ggcacattac agtttccact agtggattag
     4981 cacacaaaat caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc
     5041 acgcgccgaa taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg
     5101 agaaattgat ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg
     5161 aatatatcat gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc
     5221 tattaaaaga taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg
     5281 aacatgatca atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga
     5341 agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt
     5401 gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc
     5461 gttttttcaa ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat
     5521 aagagaaaag attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc
     5581 aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt
     5641 taactgattt tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca
     5701 agaatggcct gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt
     5761 gttgccatgg ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg
     5821 gttaacgata ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg
     5881 aactgctgat ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg
     5941 tatccctcca ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa
     6001 ttatagttat tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg
     6061 acttgttcaa ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca
     6121 taagtcaaga gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag
     6181 cctagatcat cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca
     6241 atacctgccc ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg
     6301 tgtacaggtg gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg
     6361 acacataccg cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt
     6421 ttagctagtt tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca
     6481 aacaggacgt ctgctttttg gacactttca tgctggatca actgttcaat atttccataa
     6541 gttgaattac ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact
     6601 tttattttgt tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc
     6661 ccatattctt tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt
     6721 ggtaagaaaa cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa
     6781 ttttaaagag tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag
     6841 gataagtgat gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac
     6901 gaacaagtac aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct
     6961 accgattccc tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat
     7021 gaaaacaggt tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat
     7081 atcatgatga acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt
     7141 ttgaataaag tgtttctgta taattttttt attttataag taagataaat aatgaaagaa
     7201 tagagatact gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg
     7261 actgttagaa taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta
     7321 ataatccagt attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat
     7381 atggagacag ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag
     7441 cagcggtaga ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca
     7501 caggttggga attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta
     7561 ctcgattagt caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt
     7621 taggtacgga tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata
     7681 catttgataa agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa
     7741 aattaggtta taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata
     7801 tttggatggc aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga
     7861 cagaagtaat cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DACCCS010000290         7482 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.guided.104, whole genome shotgun sequence.
ACCESSION   DACCCS010000290 DACCCS010000000
VERSION     DACCCS010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.guided.104"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            109..192
                     /locus_tag="IVK82_003138"
     CDS             109..192
                     /locus_tag="IVK82_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP6394111.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="IVK82_003139"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="IVK82_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP6394112.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="IVK82_003140"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="IVK82_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1647..2312
                     /locus_tag="IVK82_003141"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="IVK82_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2348..2593
                     /locus_tag="IVK82_003142"
     CDS             2348..2593
                     /locus_tag="IVK82_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6394113.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2696..3565
                     /locus_tag="IVK82_003143"
     CDS             2696..3565
                     /locus_tag="IVK82_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP6394114.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3546..4280
                     /locus_tag="IVK82_003144"
     CDS             3546..4280
                     /locus_tag="IVK82_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6394115.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4313..5225
                     /gene="ant(6)"
                     /locus_tag="IVK82_003145"
                     /pseudo
     CDS             4313..5225
                     /gene="ant(6)"
                     /locus_tag="IVK82_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5222..5764
                     /gene="sat4"
                     /locus_tag="IVK82_003146"
     CDS             5222..5764
                     /gene="sat4"
                     /locus_tag="IVK82_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP6394116.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5857..6651
                     /locus_tag="IVK82_003147"
                     /note="aph(3')-IIIa"
     CDS             5857..6651
                     /locus_tag="IVK82_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP6394117.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6759..6947)
                     /locus_tag="IVK82_003148"
                     /pseudo
     CDS             complement(6759..6947)
                     /locus_tag="IVK82_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6927..7469
                     /locus_tag="IVK82_003149"
     CDS             6927..7469
                     /locus_tag="IVK82_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6394118.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         2623 a         1131 c         1567 g         2161 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa ggggtttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa ctgaaaatct
     2401 aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg ggaataggat
     2461 acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta gcaaaatgaa
     2521 tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata aaaagacaat
     2581 tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc
     2641 aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat gcttgatggt
     2701 agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata tagaaggtat
     2761 tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata tagatatcgg
     2821 catctattac aattcagaat catttgacat aaatactatt aatcaattcg ctacaaagct
     2881 ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg attggattaa
     2941 tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac gtgatattaa
     3001 acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca attatcagac
     3061 tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga ttagcaaaat
     3121 actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa cttatcccaa
     3181 tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct ctttaatgtt
     3241 tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg tttttcgtat
     3301 agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta tcaacgaaaa
     3361 gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata ccgagaaggt
     3421 aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca tgaccgagaa
     3481 gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     3541 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     3601 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     3661 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat
     3721 atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg
     3781 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
     3841 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
     3901 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
     3961 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
     4021 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
     4081 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
     4141 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
     4201 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
     4261 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
     4321 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gaacgtattc
     4381 gaattgtgac ccttgagggg tcacgcgcaa atattaatat acctaaagat gaatttcagg
     4441 attatgatat tacatatttt gtaagtgata tagaaccgtt tatatctaat gatgactggc
     4501 ttaatcaatt tgggaatata ataatgatgc aaaagccgga ggatatggaa ttattcccac
     4561 ctgaagaaaa gggattttcc tatcttatgc tatttgatga ttacaataaa attgatctta
     4621 ccttattgcc cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc
     4681 taattgataa agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg
     4741 taagaaagcc aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac
     4801 cttatgttat taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc
     4861 agattgttcg ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct
     4921 ttaaattaag tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt
     4981 gggagaaact tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat
     5041 ttctatgcca tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg
     5101 cctatccgga gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca
     5161 ctggtaaaac cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac
     5221 agtgattaca gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt
     5281 tgaggtgata ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt
     5341 actctatgaa gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga
     5401 ggcagattgc cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga
     5461 cgataaatgc gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga
     5521 agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat
     5581 atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa
     5641 taaccttata gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac
     5701 tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt
     5761 ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat
     5821 ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg
     5881 gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct
     5941 gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc
     6001 cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa
     6061 ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg
     6121 ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct
     6181 gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg
     6241 gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat
     6301 aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg
     6361 cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac
     6421 ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat
     6481 cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc
     6541 agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag
     6601 cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta gtacctagat
     6661 ttagatgtct aaaaagcttt aactacaagc tttttagaca tctaatcttt tctgaagtac
     6721 atccgcaact gtccatactc tgatgtttta tatcttttct aaaagttcgc tagatagagt
     6781 tctatattag agatgtaaag agttttggtg aagaggtttg tgagtatggc ttttataacc
     6841 agggtgcaat gagaagcact aacagcagct agcttaagaa cgcataggat atacttttta
     6901 tggaagtcac agaggaggaa taaaatttga acaggattaa cagagtaacc tctattctta
     6961 ttcagctgca atcaaaaaaa ataattcctg ccaaagaaat tgcacagcga tttaatataa
     7021 gcttaaggac agtttacaga gatatccgga cacttgaaga agccggaata ccaattggat
     7081 ctgaggccgg aaaaggatat tttcttgtag agggcttcct acttccgccg gtaatgttta
     7141 cagcggcgga agtgggtgca ttgattacag cagggaaatt tttaaattgc catggagatg
     7201 aatcatttat aaaggatttt gattcagcta tgtataaaat caaatctatt ttgaagcatg
     7261 gtgaaaaaaa ctatgcacag gagttggaga acagtattaa cgtgtacagc acatctggac
     7321 agaaaaatac attggcggat aacgttattg cagcaattca gactgcaatc tgcaataaaa
     7381 gggtaatatc aattcaatat cctgcatcag gagggcaaga accggaaagc agaatgatag
     7441 agccggttct gttgcaaagt tttaaataaa gaataaaatc cc
//
LOCUS       DACCCS010000291         7478 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.guided.105, whole genome shotgun sequence.
ACCESSION   DACCCS010000291 DACCCS010000000
VERSION     DACCCS010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.guided.105"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            109..192
                     /locus_tag="IVK82_003150"
     CDS             109..192
                     /locus_tag="IVK82_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP6394119.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="IVK82_003151"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="IVK82_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP6394120.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="IVK82_003152"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="IVK82_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1647..2312
                     /locus_tag="IVK82_003153"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="IVK82_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2348..2593
                     /locus_tag="IVK82_003154"
     CDS             2348..2593
                     /locus_tag="IVK82_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6394121.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2696..3565
                     /locus_tag="IVK82_003155"
     CDS             2696..3565
                     /locus_tag="IVK82_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP6394122.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3546..4280
                     /locus_tag="IVK82_003156"
     CDS             3546..4280
                     /locus_tag="IVK82_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6394123.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4313..5221
                     /locus_tag="IVK82_003157"
     CDS             4313..5221
                     /locus_tag="IVK82_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP6394124.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5218..5760
                     /gene="sat4"
                     /locus_tag="IVK82_003158"
     CDS             5218..5760
                     /gene="sat4"
                     /locus_tag="IVK82_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP6394125.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5853..6647
                     /locus_tag="IVK82_003159"
                     /note="aph(3')-IIIa"
     CDS             5853..6647
                     /locus_tag="IVK82_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP6394126.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6755..6943)
                     /locus_tag="IVK82_003160"
                     /pseudo
     CDS             complement(6755..6943)
                     /locus_tag="IVK82_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6923..7465
                     /locus_tag="IVK82_003161"
     CDS             6923..7465
                     /locus_tag="IVK82_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6394127.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         2621 a         1130 c         1566 g         2161 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa ggggtttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa ctgaaaatct
     2401 aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg ggaataggat
     2461 acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta gcaaaatgaa
     2521 tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata aaaagacaat
     2581 tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc
     2641 aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat gcttgatggt
     2701 agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata tagaaggtat
     2761 tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata tagatatcgg
     2821 catctattac aattcagaat catttgacat aaatactatt aatcaattcg ctacaaagct
     2881 ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg attggattaa
     2941 tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac gtgatattaa
     3001 acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca attatcagac
     3061 tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga ttagcaaaat
     3121 actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa cttatcccaa
     3181 tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct ctttaatgtt
     3241 tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg tttttcgtat
     3301 agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta tcaacgaaaa
     3361 gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata ccgagaaggt
     3421 aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca tgaccgagaa
     3481 gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     3541 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     3601 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     3661 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat
     3721 atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg
     3781 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
     3841 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
     3901 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
     3961 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
     4021 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
     4081 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
     4141 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
     4201 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
     4261 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
     4321 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gtattcgaat
     4381 tgtgaccctt gaggggtcac gcgcaaatat taatatacct aaagatgaat ttcaggatta
     4441 tgatattaca tattttgtaa gtgatataga accgtttata tctaatgatg actggcttaa
     4501 tcaatttggg aatataataa tgatgcaaaa gccggaggat atggaattat tcccacctga
     4561 agaaaaggga ttttcctatc ttatgctatt tgatgattac aataaaattg atcttacctt
     4621 attgcccttg gaagagttag ataattacct aaagggcgat aaattaataa aggttctaat
     4681 tgataaagat tgtagaatta aaagggacat agttccgact gatatagatt atcatgtaag
     4741 aaagccaagc gcaagggagt atgatgattg ctgcaatgaa ttttggaatg taacacctta
     4801 tgttattaaa ggattgtgcc gtaaggaaat tttatttgct attgatcatt ttaatcagat
     4861 tgttcgccat gagctgctga gaatgatatc atggaaggtc ggcatcgaaa caggctttaa
     4921 attaagtgta ggcaagaact ataagtttat tgaaaggtat atatccgagg atttgtggga
     4981 gaaacttttg tccacctacc ggatggattc ctatgaaaac atatgggaag cattatttct
     5041 atgccatcaa ttgttcaggg cggtatccgg tgaggtggcg gaaaggcttc attatgccta
     5101 tccggagtat gataggaata taacaaaata taccagggac atgtataaaa aatacactgg
     5161 taaaaccggc tgcctggata gcacatatgc cgctgatata gaagagaggc gggaacagtg
     5221 attacagaaa tgaaagcagg gcacctgaaa gatatcgata aacccagcga accatttgag
     5281 gtgataggta agattatacc gaggtatgaa aacgagaatt ggacctttac agaattactc
     5341 tatgaagcgc catatttaaa aagctaccaa gacgaagagg atgaagagga tgaggaggca
     5401 gattgccttg aatatattga caatactgat aagataatat atctttacta ccaagacgat
     5461 aaatgcgtcg gaaaagttaa actgcgaaaa aattggaacc ggtacgctta tatagaagat
     5521 atcgccgtat gtaaggattt cagggggcaa ggcataggca gcgcgcttat caatatatct
     5581 atagaatggg caaagcataa aaacttgcat ggactaatgc ttgaaaccca ggacaataac
     5641 cttatagctt gtaaattcta tcataattgt ggtttcaaaa tcggctccgt cgatactatg
     5701 ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag
     5761 aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg gggtatcttt
     5821 aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat
     5881 tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta
     5941 aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt
     6001 ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa
     6061 agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca
     6121 tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa
     6181 agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt
     6241 gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg
     6301 atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa gatccgcgcg
     6361 agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg
     6421 acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg
     6481 ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg
     6541 aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg
     6601 attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac ctagatttag
     6661 atgtctaaaa agctttaact acaagctttt tagacatcta atcttttctg aagtacatcc
     6721 gcaactgtcc atactctgat gttttatatc ttttctaaaa gttcgctaga tagagttcta
     6781 tattagagat gtaaagagtt ttggtgaaga ggtttgtgag tatggctttt ataaccaggg
     6841 tgcaatgaga agcactaaca gcagctagct taagaacgca taggatatac tttttatgga
     6901 agtcacagag gaggaataaa atttgaacag gattaacaga gtaacctcta ttcttattca
     6961 gctgcaatca aaaaaaataa ttcctgccaa agaaattgca cagcgattta atataagctt
     7021 aaggacagtt tacagagata tccggacact tgaagaagcc ggaataccaa ttggatctga
     7081 ggccggaaaa ggatattttc ttgtagaggg cttcctactt ccgccggtaa tgtttacagc
     7141 ggcggaagtg ggtgcattga ttacagcagg gaaattttta aattgccatg gagatgaatc
     7201 atttataaag gattttgatt cagctatgta taaaatcaaa tctattttga agcatggtga
     7261 aaaaaactat gcacaggagt tggagaacag tattaacgtg tacagcacat ctggacagaa
     7321 aaatacattg gcggataacg ttattgcagc aattcagact gcaatctgca ataaaagggt
     7381 aatatcaatt caatatcctg catcaggagg gcaagaaccg gaaagcagaa tgatagagcc
     7441 ggttctgttg caaagtttta aataaagaat aaaatccc
//
LOCUS       DACCCS010000292         4995 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.guided.122, whole genome shotgun sequence.
ACCESSION   DACCCS010000292 DACCCS010000000
VERSION     DACCCS010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.guided.122"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(30..2180)
                     /locus_tag="IVK82_003162"
                     /pseudo
     CDS             complement(30..2180)
                     /locus_tag="IVK82_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2326..2901
                     /locus_tag="IVK82_003163"
     CDS             2326..2901
                     /locus_tag="IVK82_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6394128.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3115..3810
                     /gene="vanR"
                     /locus_tag="IVK82_003164"
     CDS             3115..3810
                     /gene="vanR"
                     /locus_tag="IVK82_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP6394129.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3788..4942
                     /locus_tag="IVK82_003165"
     CDS             3788..4942
                     /locus_tag="IVK82_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP6394130.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1560 a          939 c          967 g         1529 t
ORIGIN      
        1 aattttcttc tttcagcttt ttatttccca cttgtaaatc ggtagatggt ggctcctttt
       61 ccagttccat aatgtggatt tgcatctgca tgtagtgatg aaacacctag ctgcattctc
      121 ataccatctg acgaagatgt tgtaccgtcg ccccaataga aaggcaattg taatttatga
      181 tgaaagttta ctaatatggc ttgggcttta ttcatggcat cttcatacat gcgccattga
      241 gatacattgg ctagttgctt atatgtaagt ccgggtgtgg cttcggccat cttgctcaag
      301 ccaatattca ttcccattcc taaaagggca gccatgataa tgattgtttc ttccttatct
      361 ggttttcgat tattggaagc atgagtgaat tgctcatgaa atcctgttat atgggccaca
      421 tccatgagta aatcagttaa ttttattctt ggtagcatct gataaaggct tgcactaaat
      481 ttttttgctt cttctggaac atctttttct aagcgtgcaa gtgatagctt tcctttttca
      541 agagaaaccc catctaactt attggaattg gcagctaacc actttaacct ttcattaaag
      601 ctgctggttc tctccgttat ataatcttcg aatgataaac taactgataa tctcgtattc
      661 cccttcgatt gattccatgt atcttccgaa aacaaatatt cctcaaaatc cctatattgt
      721 ctgctgccaa caatggaaac atctcctgcc cgaacatgct cccgaagttc tgttaaaaca
      781 gccatttcat agtaatgacg attaattgtt gtaccatcat cctcgtataa atgtcttttc
      841 catcgttttg aaataaaatc cacaggtgag tcatcaggca cttttcgctt tccagattcg
      901 ttcattcctc ggataatctc aacagcttgt aaaagtggct catttgcctt tgtagaatga
      961 aattccaata ctcttaatag cgttggcgta tattttctta gtgaataaaa ccgtttttgc
     1021 agtaagtcta aataatcata gtcggcagga cgtgcaagtt cctgagcctc ttctactgaa
     1081 gagacaaagg tattccattc aataaccgat tctaaaacct taaaaacgtc taatttttcc
     1141 tctcttgctt taattaatgc ttgtccgatg ttcgtaaagt gtataacttt ctcatttagc
     1201 tttttaccgt tttgtttctg gatttcctct tgagccttac gaccttttga taacaaacta
     1261 agtatttgcc tatcatgaat ttcaaacgct ttatccgtta gctcctgagt aagttgtaat
     1321 aaatagatgg ttaatatcga ataacgttta ttttcttgaa agtcacggaa tgcatacggc
     1381 tcgtatcttg agcctaagcg agacagctgc aacaggcggt tacggtgcaa atgactaatt
     1441 tgcactgttt ctaaatccat tcctcgtatg tattcgagtc gttctattat ttttagaaaa
     1501 gtttcgggtg aaggatgacc cggtggctct tttaaccaac ccaatatcgt tttattggat
     1561 tcggatggat gctgcgaggt aataatccct tcaagctttt ctttttgctc atttgttaga
     1621 gatttactaa ccgtattaaa tagcttcttt tcagccattg cccttgcttc ccacaccatt
     1681 ctttcaagtg tagtgatagc aggcagtata attttgtttt ttcttagaaa atctatgcat
     1741 tcatgcagta gatgaatggc atcaccattt tccaaagcta attgatgaag gtacttaaat
     1801 gtcattcgat attcactcag ggtaaaagtt acaaagtcgt attcacttcg aatttctttc
     1861 aaatgatccc aaagtgtatt ttccctttga ggataatgat caagcgagga tggactaaca
     1921 ccaatctgtt tcgatatata ttgtatgacc gaatctggga tgcttttgat atgagtgtat
     1981 ggccaaccgg gataccgaag aacagctaat tgaacagcaa atcctaaacg gttttcttcc
     2041 ctccttcgct tattaactat ttctaaatcc cgtttggaaa aagtgaagta ggtccccagt
     2101 atccattcat cttcagggat ttgcataaaa gcctgtctct gttccggtgt aagcaattct
     2161 ctacctctcg caattttcat tcagtatcat tccatttctg tattttcaat ttattagttc
     2221 aattatatat caatagagtg tactctattg atacaaatgt agtagactga taaaatcata
     2281 gttaagagcg tctcataaga cttgtctcaa aaatgaggtg atattttgcg gaaaatcggt
     2341 tatattcgtg tcagttcgac taaccagaat ccttcaagac aatttcagca gttgaacgag
     2401 atcggaatgg atattatata tgaagagaaa gtttcaggag caacaaagga tcgcgagcaa
     2461 cttcaaaaag tgttagacga tttacaggaa gatgacatca tttatgttac agacttaact
     2521 cgaatcactc gtagtacaca agatctattt gaattaatcg ataacatacg agataaaaag
     2581 gcaagtttaa aatcactaaa agatacatgg cttgatttat cagaagataa tccatacagc
     2641 caattcttaa ttactgtaat ggctggtgtt aaccaattag agcgagatct tattcggatg
     2701 agacaacgtg aagggattga attggctaag aaagaaggaa agtttaaagg tcgattaaag
     2761 aagtatcata aaaatcacgc aggaatgaat tatgcggtaa agctatataa agaaggaaat
     2821 atgactgtaa atcaaatttg tgaaattact aatgtatcta gggcttcatt atacaggaaa
     2881 ttatcagaag tgaataatta gccattctgt attccgctaa tgggcaatat ttttaaagaa
     2941 gaaaaggaaa ctataaaata ttaacagcct cctagcgatg ccgaaaagcc ctttgataaa
     3001 aaaagaatca tcatcttaag aaattcttag tcatttatta tgtaaatgct tataaattcg
     3061 gccctataat ctgataaatt attaagggca aacttatgtg aaagggtgat aactatgagc
     3121 gataaaatac ttattgtgga tgatgaacat gaaattgccg atttggttga attatactta
     3181 aaaaacgaga attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata
     3241 gacaagtctg agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt
     3301 actatctgtc aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa
     3361 gatacagagg tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag
     3421 ccctttcgcc cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa
     3481 ttcagtggag taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat
     3541 gttaacaccc atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt
     3601 tcaatactgc gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt
     3661 catgagatat ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc
     3721 cggcatttgc gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta
     3781 tggggggttg gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa
     3841 actttacatg tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg
     3901 ttcaatgatc cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga
     3961 cttaaatcac ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat
     4021 ctttatttat gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa
     4081 attcgcaaaa tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga
     4141 taaacaaatt gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa
     4201 acggactctg gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt
     4261 tgttatgtac ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag
     4321 cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac
     4381 gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta
     4441 taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca
     4501 gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc
     4561 ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat
     4621 tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct
     4681 ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct
     4741 agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg
     4801 cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta
     4861 cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga tgccagactt
     4921 ggttgataaa aggaggtcct aagagatgta tataattttg gctctttgtc aaatgatgtt
     4981 ggtaaagaga aatct
//
LOCUS       DACCCS010000293         4801 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.guided.124, whole genome shotgun sequence.
ACCESSION   DACCCS010000293 DACCCS010000000
VERSION     DACCCS010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.guided.124"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            300..1211
                     /locus_tag="IVK82_003166"
     CDS             300..1211
                     /locus_tag="IVK82_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAP6394131.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1364..1849
                     /gene="vanZ"
                     /locus_tag="IVK82_003167"
     CDS             1364..1849
                     /gene="vanZ"
                     /locus_tag="IVK82_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAP6394132.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2136..2465)
                     /locus_tag="IVK82_003168"
     CDS             complement(2136..2465)
                     /locus_tag="IVK82_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6394133.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2770..3099
                     /locus_tag="IVK82_003169"
     CDS             2770..3099
                     /locus_tag="IVK82_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6394134.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            3089..3376
                     /locus_tag="IVK82_003170"
     CDS             3089..3376
                     /locus_tag="IVK82_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6394135.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3655..4218)
                     /locus_tag="IVK82_003171"
     CDS             complement(3655..4218)
                     /locus_tag="IVK82_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6394136.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1681 a          727 c          824 g         1569 t
ORIGIN      
        1 gcggtcatta tctgcgtgcg ctgcggcaag atagcctgat aataagactg atcgcataga
       61 ggggtggtat ttcacaccgc ccattgtcaa caggcagttc agcctcgtta aattcagcat
      121 gggtatcact tatgaaaatt catctacatt ggtgataata gtaaatccag tagggcgaaa
      181 taattgactg taatttacgg ggcaaaacgg cacaatctca aacgagattg tgccgtttaa
      241 ggggaagatt ctagaaatat ttcatacttc caactatata gttaaggagg agactgaaaa
      301 tgaagaagtt gtttttttta ttgttattgt tattcttaat atacttaggt tatgactacg
      361 ttaatgaagc actgttttct caggaaaaag tcgaatttca aaattatgat caaaatccca
      421 aagaacattt agaaaatagt gggacttctg aaaataccca agagaaaaca attacagaag
      481 aacaggttta tcaaggaaat ctgctattaa tcaatagtaa atatcctgtt cgccaagaaa
      541 gtgtgaagtc agatatcgtg aatttatcta aacatgacga attaataaat ggatacgggt
      601 tgcttgatag taatatttat atgtcaaaag aaatagcaca aaaattttca gagatggtca
      661 atgatgctgt aaagggtggc gttagtcatt ttattattaa tagtggctat cgagactttg
      721 atgagcaaag tgtgctttac caagaaatgg gggctgagta tgccttacca gcaggttata
      781 gtgagcataa ttcaggttta tcactagatg taggatcaag cttgacgaaa atggaacgag
      841 cccctgaagg aaagtggata gaagaaaatg cttggaaata cgggttcatt ttacgttatc
      901 cagaggacaa aacagagtta acaggaattc aatatgaacc atggcatatt cgctatgttg
      961 gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt tctcgaggaa tatatggatt
     1021 acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg ggaaaaatat gagatctttt
     1081 attatcctgt tactaaaaat accaccattc atgtgccgac taatcttcgt tatgagatat
     1141 caggaaacaa tatagacggt gtaattgtga cagtgtttcc cggatcaaca catactaatt
     1201 caaggaggta aggatggcgg aatgaaacca acgaaattaa tgaacagcat tattgtacta
     1261 gcacttttgg ggtaacgtta gctttttaat ttaaaaccca cgttaactag gacattgcta
     1321 tactaatgat acaacttaaa caaaagaatt agaggaaatt atattgggaa aaatattatc
     1381 tagaggattg ctagctttat atttagtgac actaatctgg ttagtgttat tcaaattaca
     1441 atacaatatt ttatcagtat ttaattatca tcaaagaagt cttaacttga ctccatttac
     1501 tgctactggg aatttcagag agatgataga taatgttata atctttattc catttggctt
     1561 gcttttgaat gtcaatttta aagaaatcgg atttttacct aagtttgctt ttgtactggt
     1621 tttaagtctt acttttgaaa taattcaatt tatcttcgct attggagcga cagacataac
     1681 agatgtaatt acaaatactg ttggaggctt tcttggactg aaattatatg gtttaagcaa
     1741 taagcatatg aatcaaaaaa aattagacag agttattatt tttgtaggta tacttttgct
     1801 cgtattattg ctcgtttacc gtacccattt aagaataaat tacgtgtaag atgtctaaat
     1861 caagcaatct gatctttcat acacataaag atattgaatg aattggatta gatggaaaac
     1921 gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg aaaaccggtg ataaagtaaa
     1981 aagcttacct aacactatag taacaaagaa agcccaatta tcaattttag tgctgaggaa
     2041 ttggtctctt taataaattt ccttaacgtt gtaaatccgc attttcctga cggtaccccc
     2101 aaactaagat ttcatagata gattttctga taactttaaa aaacacgaat agcggaaagg
     2161 atgtcttcat tcgctaaaga gtaataaact acttttccat cttgacgtga ttttgccatt
     2221 ccatgttttt ttaaaaaacg taaatggtgt gacgttgtag cgacactagc agtaagtatt
     2281 tctgctaaat cacagacgca catttcctta taggtttcta aagcatagaa aattttaatt
     2341 ctagaaaaat cgctgaaaca tttccctaac acaagtaagt ttgaaaaatc tttttgatcc
     2401 aatttatttt ttattccatt tactttttct ttatgtacga cagtcacttg gcaaactttt
     2461 ttcattcatc tcacctcaca ttcaaacgat tatttgaatg aagtatacct atttgatcac
     2521 tcttttgtca atctacctta attccaatac actattataa gcaatttcat gtattgaagt
     2581 aaacttactt gcaagtgcgt ttttgattcc aagttaaaaa catattatta ttctacataa
     2641 tttcctaatc aaataaaact cctttgaaaa ttgttctaaa ggagttttat tattttgtat
     2701 ttctactaaa aatagcgtac aatgtaatta ctaagtaatt acgaaatgta aaataatgga
     2761 ggaaataata tgattacaaa aacaagaaaa caaggaaatt ctattatgtt aacggtccct
     2821 aaagaattcg atgtgcccaa tggtgttgaa gtagaagcta agcttgtaga gaatggtatc
     2881 ctttatgagt ttgtagagcc taaaaaagaa ttttttgatt ttagtgaaga tattttggct
     2941 gatattcttt cagaaggtta tagcaaacaa gagatcttaa aagaatttag aaatagaaaa
     3001 aatgcgttaa cttctgcatt taaatctatt gctgaagata ctgttgcaaa ttctaaaccg
     3061 atgacaaaag aggagttggc agcagaaatt ggcttatgag attctacctt ctaaacacgt
     3121 aataaagtat ctaaaaaaat taaaagagaa aacattaaaa gaacaatttt taacgataat
     3181 ttatgatgag atagcagttc gtcctcattc gggagaacaa aaaacgggtg atttatcagg
     3241 aatttgggct attggattca agtatgcagg tacgacatat cgcgttgcat atgaaattaa
     3301 agataatgca gtgataccta tcttactatg tggaacgcat gaaaattttt atgaacaatt
     3361 aaaaaagatt aggtaagaac ccaatgtttc aacttgagag gatagacgac actttaaaag
     3421 ataattttta acagctgaga gttgtaatac cctataattt taaaccacta acctatatat
     3481 ttatgaaaaa tagaaaaaat taaaacgcat tttaagcctc tttcaaatta aaagcttcat
     3541 ccttatgcac aaagagggct taaaatgcgt tattttatat tgttttttag taataataaa
     3601 aaaatcactc taattctccc ataaatatta tagattccta aagattaaaa tagattattc
     3661 cctattgtct tttaagtagc ggtagagtgt tgccttgcta ataccagtca tttccgtaat
     3721 ttcttttacg ctataaacct gacttttata caattttact gctcgatcaa catctttctg
     3781 gttcactcgt ggacggccac ctttacgacc tctcgcacgt gcacttttca aaccttcttt
     3841 ggtacgttca acaattaaat cacgttcgaa ctgactaaat gcttggaaga cagtcatcat
     3901 taagcgtccg tgaggcgtgc tggtatcgaa gttttctttc aagctcacca gcttaacatg
     3961 gtgttcttca aaataggtaa caaggtcaat caaatccttt gtactacgac ccaatcgaga
     4021 aaaactttct acaattaccg tatctcccgg acgaatttta tcttttaaac gatttagttg
     4081 gggacgaacc gctttggttc ccgtcatttt ctcagttagg atttcattac aattttgttc
     4141 attgagtaag tctaattgac gatttagttc ttgtgaacgt gtactcactc gggcataacc
     4201 ataaatgtat ccactcatgc ttttcttcct ttcgtacttt aattctggct tgattgtatc
     4261 ataaaaggga gttatagata catagttaat gcaacaagtt atgatacaaa atatgacctc
     4321 ttttatttaa taaaagaggt catttcattt tgtctcataa tcgattgttt ttgatacgag
     4381 tgtgtatacg aaataaacaa aaaaatctta agtaaatttc tagataccta atttaatcct
     4441 aactagtgta tcctaaagtt aataacgagt ttatcacaat gaaaattata aaatgctccc
     4501 atattagata taacatgcaa tatttgaatt agaactacaa aatttattcg ttttataaag
     4561 taaataatgt tgagatacta aggaatagaa tattataagg aattgagctg aatgtattgg
     4621 ataaaaaaag aataacttta ttagagatgg actataaaaa tgctcgtaat tttcttctga
     4681 agtctgaaag ctatttgaat gctcagctac cagaatatat tagttttgac gaaactttaa
     4741 aatctgctaa aaaagtttta acttcgccgt ttgtataatt aagcgagaca aataaaaaag
     4801 c
//
LOCUS       DACCCS010000294         2998 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.guided.155, whole genome shotgun sequence.
ACCESSION   DACCCS010000294 DACCCS010000000
VERSION     DACCCS010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.guided.155"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            244..1212
                     /locus_tag="IVK82_003172"
     CDS             244..1212
                     /locus_tag="IVK82_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP6394137.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1205..2236
                     /gene="vanA"
                     /locus_tag="IVK82_003173"
     CDS             1205..2236
                     /gene="vanA"
                     /locus_tag="IVK82_003173"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP6394138.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2242..2850
                     /locus_tag="IVK82_003174"
     CDS             2242..2850
                     /locus_tag="IVK82_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP6394139.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          893 a          574 c          722 g          809 t
ORIGIN      
        1 tgctggtgtc atccaccaac atcaaaaagt atagagccat aattttttag gaaaatctca
       61 aggttatctt tactttttct taggaaatta acaatttaat attaagaaac ggctcgttct
      121 tacacggtag acttaatacc gtaagaacga gccgttttcg ttcttcagag aaagatttga
      181 caagattacc attggcatcc ccgttttatt tggtgccttt cacagaaagg gttggtctta
      241 attatgaata acatcggcat tactgtttat ggatgtgagc aggatgaggc agatgcattc
      301 catgctcttt cgcctcgctt tggcgttatg gcaacgataa ttaacgccaa cgtgtcggaa
      361 tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg tgggacataa atcagagatt
      421 tccgcctcta ttcttcttgc gctgaagaga gccggtgtga aatatatttc tacccgaagc
      481 atcggctgca atcatataga tacaactgct gctaagagaa tgggcatcac tgtcgacaat
      541 gtggcgtact cgccggatag cgttgccgat tatactatga tgctaattct tatggcagta
      601 cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg atttcaggtt ggacagcgac
      661 cgtggcaagg tactcagcga catgacagtt ggtgtggtgg gaacgggcca gataggcaaa
      721 gcggttattg agcggctgcg aggatttgga tgtaaagtgt tggcttatag tcgcagccga
      781 agtatagagg taaactatgt accgtttgat gagttgctgc aaaatagcga tatcgttacg
      841 cttcatgtgc cgctcaatac ggatacgcac tatattatca gccacgaaca aatacagaga
      901 atgaagcaag gagcatttct tatcaatact gggcgcggtc cacttgtaga tacctatgag
      961 ttggttaaag cattagaaaa cgggaaactg ggcggtgccg cattggatgt attggaagga
     1021 gaggaagagt ttttctactc tgattgcacc caaaaaccaa ttgataatca atttttactt
     1081 aaacttcaaa gaatgcctaa cgtgataatc acaccgcata cggcctatta taccgagcaa
     1141 gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt tggattttga aaggagacag
     1201 gagcatgaat agaataaaag ttgcaatact gtttgggggt tgctcagagg agcatgacgt
     1261 atcggtaaaa tctgcaatag agatagccgc taacattaat aaagaaaaat acgagccgtt
     1321 atacattgga attacgaaat ctggtgtatg gaaaatgtgc gaaaaacctt gcgcggaatg
     1381 ggaaaacgac aattgctatt cagctgtact ctcgccggat aaaaaaatgc acggattact
     1441 tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat gtagcatttt cagctttgca
     1501 tggcaagtca ggtgaagatg gatccataca aggtctgttt gaattgtccg gtatcccttt
     1561 tgtaggctgc gatattcaaa gctcagcaat ttgtatggac aaatcgttga catacatcgt
     1621 tgcgaaaaat gctgggatag ctactcccgc cttttgggtt attaataaag atgataggcc
     1681 ggtggcagct acgtttacct atcctgtttt tgttaagccg gcgcgttcag gctcatcctt
     1741 cggtgtgaaa aaagtcaata gcgcggacga attggactac gcaattgaat cggcaagaca
     1801 atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc tgtgaggtcg gttgtgcggt
     1861 attgggaaac agtgccgcgt tagctgttgg cgaggtggac caaatcaggc tgcagtacgg
     1921 aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc tctgaaaacg cagttataac
     1981 cgttcccgca gacctttcag cagaggagcg aggacggata caggaaacgg caaaaaaaat
     2041 atataaagcg ctcggctgta gaggtctagc ccgtgtggat atgtttttac aagataacgg
     2101 ccgcattgta ctgaacgaag tcaatactct gcccggtttc acgtcataca gtcgttatcc
     2161 ccgtatgatg gccgctgcag gtattgcact tcccgaactg attgaccgct tgatcgtatt
     2221 agcgttaaag gggtgataag catggaaata ggatttactt ttttagatga aatagtacac
     2281 ggtgttcgtt gggacgctaa atatgccact tgggataatt tcaccggaaa accggttgac
     2341 ggttatgaag taaatcgcat tgtagggaca tacgagttgg ctgaatcgct tttgaaggca
     2401 aaagaactgg ctgctaccca agggtacgga ttgcttctat gggacggtta ccgtcctaat
     2461 cgtgctgtaa actgttttat gcaatgggct gcacagccgg aaaataacct gacaaaggaa
     2521 agttattatc ccaatattga ccgaactgag atgatttcaa aaggatacgt ggcttcaaaa
     2581 tcaagccata gccgcggcag tgccattgat cttacgcttt atcgattaga cacgggtgag
     2641 cttgtaccaa tggggagccg atttgatttt atggatgaac gctctcatca tgcggcaaat
     2701 ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc gctccatcat ggaaaacagt
     2761 gggtttgaag catatagcct cgaatggtgg cactatgtat taagagacga accatacccc
     2821 aatagctatt ttgatttccc cgttaaataa acttttaacc gttgcacgga caaactatat
     2881 aagctaactc tttcggcagg aaacccgacg tatgtaactg gttcttaggg aatttatata
     2941 tagtagatag tattgaagag gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DACCCS010000295         1465 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG22-13-B
            SAMN13750725-rid10751963.guided.188, whole genome shotgun sequence.
ACCESSION   DACCCS010000295 DACCCS010000000
VERSION     DACCCS010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750725
            Sequence Read Archive: SRR10270770
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860345.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860345.1
            Genome Coverage       :: 316x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 20:50:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,175
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,040
            CDSs (with protein)               :: 3,040
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 38 of 71
            Pseudo Genes (internal stop)      :: 11 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750725-rid10751963.guided.188"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG22-13-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1348
                     /locus_tag="IVK82_003175"
     CDS             <1..1348
                     /locus_tag="IVK82_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAP6394140.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLR
                     QMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE
                     GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGE
                     DILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          622 a          117 c          246 g          480 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      721 actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc
      781 agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac aaatgcacgg
      841 tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg tattagaaga
      901 gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa aagattatat
      961 agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa agtgtttatg
     1021 ccataatgat tttagttgta atcatctatt gttagatggc aataatagat taactggaat
     1081 aattgatttt ggagattctg gaattataga tgaatattgt gattttatat acttacttga
     1141 agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa tgtatggaaa
     1201 tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt atcctattga
     1261 aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg gtagaaaaga
     1321 aatttataaa aggacttata aagattgatt atataatata tgaaaagcta ttataaaaga
     1381 cattagtatt aaatagttta aaaaaatgaa aaataataaa ggaagtgagt caagtccaga
     1441 ctcctgtgta aaatgctata caatg
//
